United States
        Environmental Protection
        Agency
Science Advisory
Board (1400)
SABEC95021F
SEPTEMBER 1995
£PA RE-EVALUATING
       DIOXIN
           >nce Advisory Boai
           iew of EPA's Reas
       Of Dioxin And Dioxin-Likd Compounds
         U.S. Environmental Prote'
         Region VII
         Information Resource Centdp
         901 N. 5th Sty
         Kansas City,

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EPA REGION VII IRC
   099206

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                                                                 PAGE  i
                                NOTICE
This report has been written as a part of the activities of the Science Advisory Board,
a public advisory group providing extramural scientific information and advice to the
Administrator and other officials of the Environmental Protection Agency. The Board
is structured to provide balanced, expert assessment of scientific matters relating to
problems facing the Agency.  This report has not been reviewed for approval by the
Agency and, therefore, the contents of this report do not necessarily represent the
views and policies of the Environmental  Protection  Agency, nor of other agencies in
the Executive Branch of the Federal government, nor does mention of trade names or
commercial products constitute a recommendation  for use.
         COPIES OF THIS REPORT MAY BE ORDERED BY MAIL FROM:

                    THE SCIENCE ADVISORY BOARD (1400)
                    U.S. EPA
                    401 M ST. SW
                    WASHINGTON DC 20460

                    OR BY TELEPHONE AT 202-260-8414
REASSESSING DIOXIN

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PA GE  II   ••'••^^^^^^••'^^•••••^^^^^^^^^^^^•^^^^^•••^•^^^^••^••^^•^•^^^^^••^^••^^•^^^^•ii^

                                   ABSTRACT

       The Dioxin Reassessment Review Committee (DRRC)  met on May 15-16, 1995 to hear
briefings by EPA staff and public comments on EPA's reassessment of dioxin and dioxin-like
compounds. The DDRC addressed 43 health and exposure-related issues.

       The Committee commended EPA for doing a very credible and thorough job assembling,
integrating, and analyzing a very large body of data on dioxin emissions, environmental levels,
exposures, and body burdens. The Committee's recommendations largely addresse refine-
ments, not substantive revisions.  The Committee agrees that current levels of dioxin-like
compounds in the environment derive primarily from anthropogenic sources and that the
air/plant/animal pathway is probably the primary way in which humans are exposed, and
supports the use of Toxic Equivalencies (TEQ) for exposure analysis.

       The Health Assessment draft provides a comprehensive review of the scientific literature
on the biology of dioxin. The Committee is confident that final versions of Chapters One
through Seven will not need further review by the SAB.  The Committee agrees with the use of
the TEFs as a basis for developing an overall index of public health risk, but their practical
application depends on the reliability of the TEFs and the availability of representative and
reliable exposure data.

       Chapter Eight, on modeling, reflects a great deal of effort, but several Members of the
Committee found the exposition of important points to be unclear.  Chapter Eight is also
weakened by its reliance on the standard EPA default assumption of a linear non-threshold
model for carcinogenic risk.

       Almost all the Members of the Committee concur with EPA's judgment that dioxin, under
some conditions of exposure, is likely to increase human cancer incidence.

       Chapter Nine, on risk assessment, displays both strengths and weaknesses, and needs
to be revised considerably to deal with the weaknesses.


KEYWORDS: dioxin; TCDD; carcinogen; Ah  receptor; TEQ; TEF; reassessment; anthropo-
genic; incineration; food
                                                     THE SCIENCE ADVISORY BOARD

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                                                                       :  PAGE  Hi

                    U.S. ENVIRONMENTAL PROTECTION AGENCY
                            SCIENCE ADVISORY BOARD
                          DIOXIN REASSESSMENT REVIEW
                                  May 15-16, 1995

                                  HEALTH PANEL

CHAIR
Dr. Morton Lippmann, New York University Medical Center, Institute of Environmental Medicine,
      Tuxedo, NY

MEMBERS
Dr. William B. Bunn, Mobil Administrative Services Company, Inc., Princeton, NJ

Dr. Kenny S. Crump, K S Crump Division, Ruston, LA

Dr. Ernest E. McConnell, Raleigh, NC

Dr. Henry C. Pitot, McArdle Laboratory for Cancer Research, Madison, WS

CONSULTANTS
Dr. Richard W. Clapp, Boston University School of Public Health, Boston, MA

Dr. John Doull, University of Kansas, Kansas City, KS

Dr. Ronald W. Estabrook, The University of Texas, Dallas, TX

Dr. John Graham, Harvard Center for Risk Analysis, Boston, MA

Dr. William Greenlee, Purdue University, West Lafayette, IN1

Dr. Norbert Kaminski. Michigan State University, East Lansing, Ml

Dr. Thomas Mack, University of Southern California, Los Angles, CA

Dr. John McLachlan, Tulane University, New Orleans, LA

Dr. David Ozonoff, Boston University School of Public Health, Boston, MA

Dr. Gabriel Plaa, Department of Pharmacology, Montreal, Quebec, Canada

Dr. Donald Reed, Oregon State University, Corvallis, OR

Dr. Knut Ringen, Center to Protect Workers' Rights, Washington, DC
        Now at the University of Massachusetts, Worcester, MA.

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PAGE   iv                                               ~

Dr. Allen Silverstone, State University of New York Health Science Center,
      Syracuse, NY

Dr. Sidney Stohs, Creighton University, Omaha, NE

Dr. Bernard Weiss, University of Rochester. Rochester, NY

Dr. Hanspeter Witschi, University of California, Davis, CA

Dr. Timothy Zacharewski, University of Western Ontario, London, Ontario, Canada

FEDERAL EXPERTS
Dr. Michael Gough, U.S. Congress, Office of Technology Assessment. Washington, DC

Dr. Michael I. Luster, National Institute of Environmental Health Sciences, Research Triangle
      Park,  NC

Dr. Thomas Umbreit, Food  and Drug Administration, Rockville, MD

DESIGNATED FEDERAL OFFICIAL
Mr. Samuel Rondberg, Science Advisory Board (1400F), U.S. Environmental Protection
      Agency, Washington, D.C. 20460


                                 EXPOSURE PANEL

CHAIR
Dr. Joan Daisey, Lawrence Berkeley Laboratory, Berkeley, CA

MEMBERS
Dr. Paul Bailey, Mobil Company,     Princeton, NJ

Dr. Robert Hazen, Bureau of Risk Assessment, State of New Jersey, Trenton, NJ

Dr. Kai-Shen  Liu, California Department of Health Services, Berkeley, CA

Dr. Thomas E. McKone, University of California, Davis, CA
Dr. Maria Morandi, University of Texas Health Science Center at Houston, Houston, TX

Dr. Jonathan  M. Samet, Johns Hopkins University, Baltimore, MD

Dr. William Randall Seeker, Energy & Environmental, Research Corp., Irvine, CA

Mr. Ron White, American Lung Association, Washington, DC

CONSULTANTS
Dr. Ronald Hites, Indiana University,  Bloomington, IN
                                                   THE SCIENCE ADVISORY BOARD

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                                                                  = PAGE  v

Dr. Nancy Kim, New York State Department of Health, Albany, NY

Dr. Dennis Paustenbach, McLaren/Hart/ChemRisk, Alameda, CA

Dr. John Jake Ryan, Bureau of Chemical Safety, Ottawa, Ontario, Canada

Dr. Valerie Thomas, Princeton University, Princeton, NJ

DESIGNATED FEDERAL OFFICIALS
Mr. Samuel Rondberg, Science Advisory Board (1400F) U.S. Environmental Protection Agency,
      Washington, DC 20460  ,

Mr. A. Robert Flaak, Science Advisory Board (1400F), U.S. Environmental Protection Agency,
      Washington, DC 20460
STAFF SECRETARY
Ms. Mary L Winston, Environmental Protection Agency, Science Advisory Board (HOOF)
      Washington,  DC 20460

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PAGE   vi

                            TABLE OF CONTENTS
1.  EXECUTIVE SUMMARY	1
      1.1 Exposure Document	1
      1.2 Health Document 	3

2.  INTRODUCTION	6
      2.1 Background	6
      2.2 Charge  	7
             2.2.1  Exposure Document Charge	7
             2.2.2  Health Document Charge	10

3.  DETAILED FINDINGS-EXPOSURE DOCUMENT	18
      3.1 Sources	18
             3.1.1   Estimating and Apportioning Sources  	18
             3.1.2   Source Inventory	19
             3.1.3   Dioxin Reservoirs 	19
             3.1.4   Local/Distant Contributions to Dioxin Levels in Food	20
             3.1.5   Uncertainty in Deposition Estimates 	21
      3.2    Food  and Media Levels  	21
             3.2.1   Problems in Estimating CDD/F Levels	21
             3.2.2   Use of U.S. Food Data 	22
             3.2.3   Air/Plant/Animal Pathway and Other Food Chain Impacts	  .... 24
             3.2.4   Smoking-An Additional Potential Exposure Pathway  	25
      3.3    Uncertainty in Estimating Human Body Burdens	27
      3.4    Estimating Background Exposures via Food/Body Burden Data 	28
      3.5    Site-Specific Assessment Procedures 	28
             3.5.1   Steady-State Assumptions and Modeling  	28
             3.5.2   Mass-Balance Issues	28
             3.5.3   Model Validation Issues  	29
             3.5.4   Photolysis and Atmospheric Transport	-..,... 30
             3.5.5   Air-to-Plant Transfer	31
             3.5.6   Vapor/Particle Partitioning	32
             3.5.7   Background Exposures and Site-Specific Evaluation .	33
             3.5.8   Evaluation of Multiple Sources 	34
      3.6    Overall Scientific Foundations of the Reassessment Document	34

4.  DETAILED FINDINGS-HEALTH DOCUMENT	37
      4.1    Disposition and Pharmacokinetics Issues	37
             4.1.1   Strength of the database	37
             4.1.2   Disposition and Pharmacokinetics	38
             4.1.3  Incremental Exposures and Bioaccumulation  	40
             4.1.4  Uncertainties in Back Extrapolations of Body Burden  	40
      4.2 Mechanisms of Dioxin Action  	41
             4.2.1   Animal-to-Human Extrapolations of Receptor Structure and Function .  . 41
                                                   THE SCIENCE ADVISORY BOARD

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              ..                                                          PAGE  vii

                   TABLE OF CONTENTS (CONTINUED)

      4.3    Toxic Effects of Dioxin  	43
             4.3.1   Animal Models for estimating Human Risk	43
             4.3.2   Variations in Human Sensitivity	45
      4.4    Chloracne as an Indicator of Exposure	46
      4.5  Cancer	46
             4.5.1   Epidemiological Evidence	46
             4.5.2   Carcinogenicity of Dioxin-like Compounds	49
             4.5.3   Carcinogenic Activities of Dioxin and Dioxin-Like Compounds	50
             4.5.4   Characterization of Dioxin/Dioxin-like Compounds as Human Carcino-
                   gens 	54
      4.6    Developmental Toxicity and Animal NOAELs 	56
      4.7    Human/Animal Databases: Potential for Immunotoxicity  	59
      4.8    Other Effects	62
      4.9 Dose-Response  	62
             4.9.1   Approaches to Dose-response Determination for Cancer  	62
             4.9.2   Use of the RfD in Evaluating Incremental Exposures	65
             4.9.3   Continuum of Response Postulate 	66
      4.10   Use of Toxicity Equivalence Factors (TEFs)	67
      4.11   Laboratory Animals/ Human Response  	69
             4.11.1 Animal Data/ Weight-of- Evidence Conclusions for Human Risk	69
             4.11.2 Animal vs. Human Data 	70
      4.12   Overall Scientific Foundations of the Health Reassessment Document....... 71
             4.12.1 Evaluation of the Risk Assessment Chapter	71
             4.12.2 Evaluation of Major Conclusions	74
      4.13   Other Issues and Future Steps	78

5.  CONCLUSIONS	81
      5.1    Exposure Assessment Document	81
      5.2    Health Assessment Document	83

REFERENCES	 . 85
                                        VII

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                                                                           PAGE   1
                           1. EXECUTIVE SUMMARY
1.1  Exposure Document

       The EPA and its staff have done a very
credible and thorough job, and are commended for
assembling, integrating, and analyzing a very large
body of data on dioxin  source emissions, environ-
mental levels, exposures,  and human body burdens,
within the framework of human exposure assessment.
In general, the data and analyses have been
clearly presented, and uncertainties and limi-
tations in the extant data described.  Conse-
quently, the recommendations of the  Com-
mittee largely address refinements, correc-
tions,  and  clarifications,  not substantive
revisions.

       The reassessment identifies the  major
known  sources of dioxins and provides  a
reasonable estimate of total emissions.  The
Committee recommends that the new infor-
mation  on emissions  from incineration  of
medical waste and other sources be incorpo-
rated if  appropriate.    The Committee also
recommends adding an explicit statement to
the final document noting that the fractional
contributions of various  types  of emissions
sources to total emissions cannot be assumed
to be identical to the  fractional contributions
of those sources to human exposures.

       At present, it is difficult to evaluate the
relative contributions of local and more dis-
tant sources to the levels of dioxin  in food.
When better data become available from on-
going EPA measurements of dioxin concentra-
tions in food, the Committee suggests that the
Agency consider using  a Geographical Infor-
mation System (CIS)  for  analysis of these
data.  With  such  a  system,  the geographic
distributions of dioxin emissions sources and
dioxin levels  in food could be mapped and
quantitative  questions  asked  (and tested
statistically) regarding the probable influences
of local and more distant sources.

       In the Exposure document, total esti-
mated dioxin-like emissions for the U.S. have
been directly compared to  an estimate of the
total amount of dioxin deposited to the sur-
face of the U.S., based on available measured
deposition factors.  However, a scientifically-
valid mass balance comparison would require
estimating deposition of the emitted dioxins
using atmospheric dispersion and deposition
modeling and then comparing this estimate to
the estimate obtained from  measured and
representative deposition data.  The Commit-
tee concurs with EPA's position (Volume II, p.
3-166) that it  is not scientifically  valid to
infer, based on the simple mass balance com-
parison,  that there  are  missing sources of

  ... the recommendations of the
    Committee largely address
   refinements,  corrections, and
   clarifications, not  substantive
              revisions.

dioxins.  The Committee  also  recommends
that this  section of the document be modified
substantially  so that the simple direct mass
balance comparison is not  provided,  and that
the scientific problems with this procedure,
which are given, be modified appropriately to
reflect this revision.

       The  Committee  agrees  with   the  EPA
position that current levels of dioxin-like compounds
in the environment  are  derived  primarily from
REASSESSING DIOXIN

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PAGE  2
anthropogenic sources, and, based on available data,
that  the air-to-plant-to-animal pathway is most
probably the primary way in which the food chain is
impacted and humans are exposed.  EPA should,
however, take note of other potentially impor-
tant exposure pathways, e.g., point source-to-
water-to-fish, and cigarette smoking.  There is
also a very large gap in our understanding of
the potential atmospheric transformation of
vapor-phase dioxin-like compounds and of the
air to plant transfer coefficients of these com-
pounds.

   ... the potential contributions
   from reservoirs might indeed
    be important and that these
   sources should be evaluated
          more thoroughly.

       The document's estimate of average
dioxin exposure is reasonable, but has substan-
tial uncertainties due to limited  data, and
cannot  provide an estimate of the complete
distribution of exposures for the U.S. popula-
tion. In addition, although the body burden
data are dearly not adequate for rigorous time-
trend analysis, there is some evidence that
exposure may be decreasing in the U.S. The
Committee recommends that these points be
noted clearly and explicitly in  the Summary
volume  for the benefit of policy makers and
the public. The Committee commends and
fully supports EPA's efforts  to develop better
data on concentrations of dioxins in food and
in human tissue and regards these as very high
priority research needs.

       The reassessment document indicated
that it is possible that dioxins from historic
reservoir  sources  might  be  re-introduced
through various exposure pathways.   The
Committee agrees that the potential contribu-
tions from reservoirs might indeed be impor-
tant and that these sources should be evalu-
ated more thoroughly.

       The  Exposure  document  defines  a
"background"  exposure  based  on  existing
monitoring data obtained from sites removed
from known contaminant sources (or from
food representative of the  general  supply).
The Committee has two concerns with  the
"background exposures"  as so defined.  The
first is that this term be  used  consistently
throughout the document. The  second con-
cern is that the comparison of estimated expo-
sures  from a single planned facility to this
"background" might not be adequate if  the
region  already had a higher level of exposure
than the "background" due to the presence of
multiple existing sources.   The Committee
recommends that a "baseline" exposure assess-
ment also be made for the local area or region
for comparison to the "background," and that
the Agency consider providing guidance  for
performing "baseline"  exposure  assessments,
as well as assessments  of the exposure incre-
ment from a proposed facility.

       Finally, although  the Committee sup-
ports EPA's use of Toxic Equivalences (TEQs)
for exposure analysis, it also recommends that
EPA carefully review the draft Exposure  As-
sessment report and ensure that the congener-
specific data are used in all instances  (such as
transport,   transformation,  and  deposition
processes) in which differences in the  physical
and chemical properties of the congeners  are
likely to be important.  The Committee  has
noted several such cases in this report.
                                                     THE SCIENCE ADVISORY BOARD

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                                                                             PAGE  3
1.2  Health Document

       The document, in the first seven chap-
ters, provides a comprehensive review of the
scientific literature  on the biological  mecha-
nisms involved in the uptake of dioxin and
related  compounds;  the binding  of these
substances to receptor sites and their metabo-
lism and retention in  tissues; and cellular,
organic,  and  whole  body  responses.   The
Committee commends the EPA staff for this consid-
erable accomplishment, and has made a number of
comments and  suggestions for relatively minor
changes, corrections, and  citations to  additional
literature that  should  sharpen and  clarify  the
content of the initial seven chapters.  The Commit-
tee's most significant recommendations con-
cerning these  seven chapters center  on the
Agency's use  of Toxic Equivalency  Factors
(TEF) to address the broad range of  dioxin-
like compounds having the common property
of binding to the Ah receptor and producing
related responses in cells and whole animals.
The use of the TEFs  as a basis for developing an
overall index of public health risk is clearly justifi-
able,  but its practical application  depends on  the
reliability of the  TEFs  and the availability  of
representative and reliable, exposure data.  The
Committee calls for clarifications in the speci-
fications for TEFs of the various dioxin-like
compounds  for  various  health  outcomes  of
concern, including the development of sepa-
rate  TEFs for the major compound  classes,
i.e., 2,3,7,8-TCDD, other dibenzodioxins and
furans, and coplanar PCBs. The Committee is
confident  that final  versions  of Chapters  One
through Seven  will not need further review by the
SAB.

       The  eighth chapter on modeling is
critical to the reassessment's overall success.
The  modeling  must deal with  both  human
and laboratory animal data. The human data
are usually based on accidents and industrial
exposures and are subject to  confounding
factors such as exposures to other toxicants,
differences in population distributions of age,
sex, ethnic  background,  diet, etc.  Animal
studies often involve high-to low-dose extrap-
olations as well as cross-species extrapolation.
Both types of such data  are  inadequate, by
themselves, for estimating the human health
risks  of  chronic,  low-dose  environmental
exposures to dioxin and related compounds.
Although the modeling chapter reflects  a great deal
of effort, several Members of the Committee found
the exposition of important points to be unclear.
Chapter Eight is also weakened by its reliance on
the standard EPA default assumption of a  linear
non-threshold model for carcinogenic  risk.   The
Committee suggests that EPA consider, in future
revisions,  alternative risk models, allowing for
minimal  response at low environmental levels of
exposure, and which  would be consistent with the
body of available  physiological  (and,  with  the
opportunities now arising, pharmaco-kinetic) model-
ing of factors such  as deposition,  tissue dose, and
excretion, as well as the epidemiological, and bioas-
say data.

       ...  the assignment of the
   dioxins,  the PCBs, or PBBs  to
  one of a mutually exclusive and
    collectively  exhaustive  set of
     carcinogenicity categories
  grossly oversimplifies the state
       of the science in most
              instances...

        Vis-a-vis cancer, the Committee notes
that  all  of  the evidence available  argues
strongly  that  TCDD exerts its carcinogenic

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PAGE   4
effect primarily through its effectiveness as a
promoting agent stimulating cell replication in
a reversible manner, and inhibiting apoptosis,
both   mechanisms  presumably   mediated
through the Ah receptor and associated trans-
duction mechanisms.   TCDD is  thus not  a
complete carcinogen, and, to avoid confusion should
not be designated as such  in  the EPA document.
Almost  all Members of the Committee do concur
with EPA's judgment that 2,3,7,8-TCDD, under
some conditions of exposure,  is  likely to  increase
human  cancer incidence.2  The conclusion  with
respect to dioxin-like compounds  is less firm.  The
Committee notes that the  assignment of the
dioxins, the PCBs, or PBBs to one of a mutu-
ally exclusive and collectively exhaustive set of
carcinogenicity categories grossly oversimpli-
fies the state of the science in most instances,
excepting those compounds for which there  is
an  abundance of uniformly  consistent evi-
dence,  but that  EPA must  make  such an
assignment.   Under the  1986  EPA  cancer
guidelines,  levels  of  carcinogenic  evidence,
with mutually exclusive descriptive terms are
provided.  These  choices include Group A  —
human  carcinogen;  Bt  -probable   human
carcinogen on the  basis of limited information
from human studies as well as animal studies;
Group  B2 — probable human carcinogen on
the basis of animal studies only; Group C  —
possible human carcinogen; Group D -  not
classifiable; and Group E -- evidence of non-
carcinogenicity for humans.   In the case of
dioxin, virtually all of the Committee  believe
that the animal studies would be categorized
as "sufficient" and the studies of humans as
"limited," providing for an overall categoriza-
tion of Bp which would be expressed verbally
as "Probably Carcinogenic  to humans with
limited supporting information from human
studies."  The Committee (on  the basis of similar
effects) would support the  same  designation for
dioxin-like  materials.   PBBs  and PCBs  would
receive ratings ofBt and B2, respectively.

        Chapter Nine, on  risk assessment,
was  not as thoroughly peer-reviewed as were
the preceding chapters. It needs to be revised
considerably to reflect the changes being made
in Chapters  1-8 and the areas of weakness
discussed  below. The chapter  would greatly
benefit from an external peer review  by  a
group including some  scientists  active  in
dioxin research and individuals with outstand-
ing  credentials and  experience in basic re-
search and quantitative modeling of receptor-
mediated  processes. The  review group should
also include other scientists with broad toxico-
logical, epidemiological,   and  public health
experience to  place the  risks of dioxin and
related compounds in perspective; and,  as
observers, risk managers who have to contend
with concerns  of the larger public in address-
ing regulatory options.3

        More   specifically,  the  Committee
identified, and wishes to emphasize to  the
Agency, particular areas of both strength and
weakness  in Chapter Nine.
          One Member contends that no epidemiological
study has produced evidence that is widely accepted by the
scientific community, including the International Agency for
Research on Cancer, as being convincing for the human
carcinogenicity of dioxin.
          One Member of the Committee disagrees with
the suggested composition of the peer review group, specifi-
cally the inclusion of public health experts and risk managers.
He believes that the presence of such participants would
divert the focus of the review from science to other issues.
These comments apply also to the proposals for peer review
which appear in sections 4.1.3 and 5.2 of this report.
                                                         THE SCIENCE ADVISORY BOARD

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        Three major strengths  are apparent.
First, by focusing serious attention on  various non-
cancer effects, the Agency has dispelled any mis-
impression that EPA's risk assessment process  is
overly preoccupied with carcinogenic effects.  Second,
by evaluating an entire group of compound classes
(with a common attribute), rather than  a single
compound, the Agency responds to the generally-
mistaken criticism that its  risk assessment process
can only address issues on  a chemical-by-chemical
basis.   Third, a useful comparative perspective  is
provided in the draft conclusions where the Agency
highlights the fact that the margin of safety (between
background exposures and levels of exposure where
effects have been observed in test animals) for dioxin-
like compounds is smaller than that EPA usually
accepts for many other compounds.
             —  '                   PAGE  5

         Three  major  weaknesses  were also
noted. First,  almost all of the  Members  of the
Committee   conclude  that  the  presentation  of
scientific findings portrayed in  the draft docu-
ment's  conclusions is not  balanced vis-a-vis the
possible risks posed by exposure to  dioxin, with a
tendency to overstate the possibility for danger.4
Second,  important uncertainties associated with
the Agency's conclusions are not fully  identified
and  are  not  subjected  to  feasible   analyses.
Finally,  the  characterization of non-cancer risk  is
not  performed in a   manner that  can  facilitate
meaningful analysis of the incremental benefits of
risk  management alternatives.
                                                               However, several Members of the Committee
                                                     do not agree with this statement and regard the EPA
                                                     presentation as appropriately conservative within the context
                                                     of public health protection.

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                                                                           PAGE  81
                                5. CONCLUSIONS
5.1    Exposure Assessment Docu-
       ment

       EPA  has done a very credible  and
thorough job on a large and complex task.
The Agency is commended on the work
that has been  done to assemble, integrate,
and analyze a  very large body  of  data on
source   emissions,  environmental  levels,
exposures, and human body burdens  in a
framework of human  exposure assessment.
In so doing, they have uncovered key data
gaps and issues, developed some reasonable
priorities  for  future  efforts,  and begun
research efforts to address key information
gaps.   In general, the work has been clearly
presented, and  uncertainties and limitations
in  the  data are  generally  well  described.
Thus, the recommendations  of the Commit-
tee largely address refinements, corrections
and  clarifications  that  should be  made to
the  Exposure  Assessment  draft  document
rather  than  substantive revisions.

       In general, the  emissions inventory
has identified the major known sources of
dioxins  and  provided a reasonable  estimate
of total emissions, given the available data.
The Committee recommends that  the  new
information  on emissions from incineration
of medical  waste, which became  available
after this draft document was prepared, be
reviewed  and  the emissions  estimates  be
revised if appropriate.  The  Committee  also
recommends  adding an explicit statement to
the final document  noting that the  frac-
tional  contributions  of various types of
emissions sources  to total emissions cannot
be assumed  to  be  identical to the fractional
contributions of those sources  to  human
exposures.
      At present, it is  difficult to  evaluate
the relative  contributions of local and more
distant  sources to  the  levels of dioxin in
food.  When better data become available
from on-going EPA measurements of dioxin
concentrations  in  food,  the  Committee
suggests  that the Agency considering using
a  Geographical Information  System  (CIS)
for analysis of these data.   With such  a
system,  the  geographic  distributions  of
dioxin emissions sources  and dioxin  levels in
food  could be  mapped  and quantitative
questions asked  (and  tested  statistically)
regarding the probable  influences  of  local
and more distant  sources.

      In the  Exposure  document,   total
estimated dioxin-like emissions for the U.S.
have been directly compared  to an estimate
of the  total amount of  dioxin deposited to
the surface  of the U.S., based on available
measured deposition  factors.   However,  a
scientifically-valid mass  balance  comparison
would  require estimating deposition of the
emitted dioxins using atmospheric dispersion
and  deposition modeling and then  compar-
ing this  estimate  to the estimate obtained
from measured and representative deposition
data.  The Committee's concurs with  EPA's
position (Volume II, p. 3-166) that it is not
scientifically valid to infer that there  are
(based  on the  simple mass balance compari-
son) missing sources of  dioxins.  The  Com-
mittee  also recommends that this section of
the document be modified substantially so
that the simple direct mass balance compari-
son is  not provided, and that the scientific
problems with this procedure, which  are
given,  be modified appropriately to reflect
this revision.
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PAGE  82  =
      The Committee agrees that the avail-
able scientific evidence strongly indicates that
current levels of dioxin-like compounds in the
environment  derive  from  anthropogenic
sources  and that the air-to-plant-to-animal
pathway is most probably the primary way in
which the food chain is impacted and humans
are exposed.  However, the environmental
data are limited and EPA should not  loose
sight of other potentially important exposure
pathways that may impact on some parts of
the population, e.g., point source to water to
fish,  and  cigarette smoking.   Furthermore,
there is a very large gap in our understanding
of the potential atmospheric transformation of
vapor-phase dioxin-like compounds and of the
air to plant transfer coefficients  of these
compounds.  Environmental measurements of
deposition  of  paniculate  and vapor-phase
dioxin-like compounds to the surface are also
extremely limited,  although we understand
that there'are now some efforts to address this
lack.

      The reassessment document indicated
that  it is possible that dioxins  from historic
reservoir sources might  be  re-introduced
through various exposure pathways.  The
Committee agreed that the potential contribu-
tions from reservoirs might indeed be impor-
tant  and that these sources should be evalu-
ated more thoroughly.

      The  Exposure document defines  a
"background"  exposure  based  on  existing
monitoring data obtained from sites removed
from  known contaminant sources  (or from
food representative  of  the general supply).
The  Committee had two concerns  with the
"background exposures" as so defined. The
first  is that this term be  used consistently
throughout the document. The second con-
cern  was that the  comparison of estimated
exposures from a single planned facility to this
"background" might not be adequate if the
region already had a higher level of exposure
than the "background" due to the presence of
multiple  existing  sources.  The Committee
recommended that a  "baseline"  exposure
assessment also be made for the local area or
region for comparison to the "background,"
and that the Agency consider providing guid-
ance  for  performing  "baseline"   exposure
assessments, as well as assessments  of the
exposure increment from a proposed facility.

      Although   the  Committee  supports
EPA's use of TEQs  for exposure analysis, it
also  recommends  that EPA carefully  review

      ... the available scientific
  evidence strongly indicates that
     current levels of dioxin-like
  compounds in the  environment
     derive from anthropogenic
	sources...	
the  draft Exposure Assessment report and
ensure that  trie congener-specific  data are
used in all instances (such as transport, trans-
formation, and deposition processes) in which
differences in the physical and chemical prop-
erties of the congeners are likely to be impor-
tant.  The Committee has noted several such
cases in this report.

      The Exposure Assessment  document
provides  a reasonable central  estimate  of
dioxin exposure: but the estimate has substan-
tial uncertainties at this time because of to the
very  limited  data that are  available. The
assessment does  not  (and, given  currently
available data, cannot) provide an estimate of
the complete distribution of exposures  for the
U.S. population which is needed to provide a
strong scientific basis for a judging whether
                                                    THE SCIENCE ADVISORY BOARD

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                                                                          PAGE   83
there are currently significant adverse health
risks to the U.S. population from the dioxins.
Nor are the body burden  data adequate for
time trend analysis. The  Committee com-
mends and fully supports EPA's  efforts  to
develop more current and representative data
on concentrations of dioxins in food and in
human tissue.  These are  very high priority
research needs.

5.2   Health Assessment Document

      The first seven chapters  of this three-
volume  document  present a comprehensive
and  careful review of the scientific literature
on  the  biological  mechanisms leading to:
uptake of dioxin and related compounds; their
binding to receptor  sites;  their metabolism
and retention in tissues; and cellular, organic,
and whole body responses. The Committee
commends the EPA staff for this considerable
accomplishment, and has made a number of
comments and suggestions for relatively minor
changes,  corrections, and  citations to addi-
tional literature that should  sharpen  and
clarify the content of these chapters further.
It is confident that EPA will use this guidance
to produce improved final versions of these
chapters that will not need further review by
SAB.

      The document represents a departure
from the  earlier EPA risk  assessment for
dioxin, which dealt primarily with  2,3,7,8-
TCDD.    In addressing a broad  range  of
dioxin-like compounds having  the  common
property of binding to the Ah  receptor, and
producing related responses in cells and whole
animals, it creates opportunities for a holistic
assessment of the cumulative impacts of these
broadly distributed anthropogenic pollutants.
Thus, while the environmental concentrations
of each compound alone may be too low to
produce  effects  of  concern, the  combined
exposures may  be  producing effects  that
warrant concern.  The use of the concept of
toxicity equivalence factors (TEFs), and the
concentrations of the compounds in foods and
environmental media, to produce an overall
index of public health risk is clearly justifiable.
Its practical application depends on the reli-
ability of the  TEFs and  the  availability  of
representative  and  reliable exposure  data.
This review of the Health Assessment Docu-
ment calls for clarifications in the specifica-
tions for TEFs of the various dioxin-like com-
pounds  for various health  outcomes  of con-
cern.  Having such specifications  on TEFs,
combined with exposure data for the specific
compounds,  the  contributions to the overall
risk of the various compounds and compound
classes can then be determined with sufficient
confidence for risk management decisions.  In
other words, when the risk manager concludes
that the  overall health risk from dioxin and
related  compounds  requires a decision  to
reduce the risk, such decisions can be based
on knowledge  of which control options can
produce the greatest increments of risk reduc-
tion for these  several classes of compounds
covered in this document.

     The document ... creates
     opportunities for a holistic
  assessment of the cumulative
      impacts of these broadly
     distributed anthropogenic
	pollutants.	

      It is  on the basis  of the  preceding
discussion that the Committee calls for more
explicit treatment, in the revised document, of
the  major  compound classes,  i.e., 2,3,7,8-
TCDD, other dibenzodioxins and furans, and
coplanar PCBs.  Each has different  sources
REASSESSING DIOXIN

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PAGE  84
and  options for source and exposure reduc-
tion.

      The eighth chapter on modeling is a
critical one in the document.  The interpreta-
tion of the available bioeffects data  from
controlled exposure studies in the laboratory
depends largely on cross-species and high dose
to-low dose extrapolations.   The interpreta-
tion of the human experience, largely (but not
exclusively)  from relatively high dose indus-
trial workers exposures and acute exposures of
populations to accidental releases,  requires
knowledge of,  and corrections for,  dose-re-
lated responses,  and for the influence of
confounding factors  such as  exposures to
other  toxicants,  differences  in  population
distributions of age, sex, ethnic background,
diet, etc.  Since each set of relevant data is
inadequate, by itself, for estimating the hu-
man health risks of chronic, low-dose environ-
mental exposures to dioxin and related com-
pounds, models that are based on all of the
relevant data are essential,

      The  modeling chapter  does  an ade-
quate job summarizing the current  state-of-
the-art of modeling  relevant to  dioxin and
related  compounds.   Its major deficiency,
from the perspective of the SAB review Com-
mittee, was its reliance on the standard EPA
default assumption of a linear non-threshold
model for carcinogenic  risk. Many Members
of the  Committee were impressed  by the
possibility of using the available data, primar-
ily the low-dose data of Kociba et al. (1976;
1978; 1979) for rats,  and the Bertazzi et al.
(1993) data  for humans, to construct an
alternate model allowing for minimal response
at low environmental  levels of exposure that
would be consistent with the body of available
epidemiological and bioassay data, and recom-
mend that the feasibility of such a model be
discussed in the revised draft chapter.

       The  final chapter on risk assessment
had, of necessity, the limitations imposed on
it by its reliance on the  contents of the first
eight chapters.  Also,  having been  prepared
after the external peer reviews devoted to the
earlier chapters, it  was  not as thoroughly
reviewed as were the preceding chapters.  It
needs  to be revised to  reflect, the changes
being made in Chapters  1-8, and the areas of
weakness discussed above in Sections 4.12.1
and  4.12.2.  Chapter 9 would greatly benefit
from an external peer review by a group in-
cluding: scientists active  in dioxin  research;
other scientists with broad toxicological (in-
cluding experience  in  basic  research  and
modeling of receptor-mediated processes); and
scientists  with  epidemiological and  public
health perspectives, to place the risks of di-
oxin and related compounds in perspective.  It
may also be desirable  to invite, as observers,
risk managers, who may have to contend with
concerns of the larger public in  addressing
regulatory options for these compounds.30
                                                     30
                                                        Several Committee Members believe that EPA
                                               should consider in a threshold model the possibility of other
                                               toxic effects occurring in the "threshold region."
                                                       THE SCIENCE ADVISORY BOARD

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                                                                      =  PAGE   85

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                                                                       = PAGE  91

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PAGE  92  ^^^^^^^^^^^^^^^^^^^=^^^^^^^^^^=

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                                                                       HZZ  PAGE  93

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                                                                             PAGE   95

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PAGE  96                                                                      =

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PAGE  6
                               2.  INTRODUCTION
2.1  Background
       Dioxins are a group of anthropogenic
chemical compounds created as  unintended
by-products through a number of activities
including: combustion, certain types of chemi-
cal manufacture,  chlorine bleaching of pulp
and paper, and other industrial processes. For
the purpose of this review, the terms "dioxin"
and "dioxin-like compounds" are used to refer
to the family of dioxins, furans, and dioxin-like
PCBs, and comprises 2,3,7,8-TCDD and other
2,3,7,8-substituted  dioxins,   2,3,7,8-substi-
tuted furans, and those PCB congeners with at
least four chlorine atoms which can assume a
planar conformation  and have  dioxin-like
activity, including the non ortho, mono ortho,
and a few di ortho PCB congeners.  Dioxins are
produced in very  small quantities (the emis-
sions estimates in Table II-3 of Volume 1 of
the Exposure Assessment sum to about 25
pounds or 11.3 kilograms annually) compared
to other pollutants ; however, because they are
thought to be highly toxic, they have been
treated as significant environmental pollutants
since the early 1970's.

       In 1988, EPA released two documents
addressing risks from dioxins (A Cancer Risk-
Specific Dose Estimate for 2,3,7,8-TCDD and
Estimating Exposure  to  2,3,7,8-TCDD) and
requested that the Science Advisory  Board
(SAB) review  them. The SAB report (SAB,
1989), released in November 1989, although
not agreeing with several of the conclusions in
the two  documents, concluded  that  "both
documents were carefully constructed and well
written." The SAB report concluded a recom-
mendation  to  "..follow up on  this excellent
start." by developing and validating new mod-
els  for human exposure and for cancer and
non-cancer risk  endpoints,  and  to  pursue
active research programs to resolve questions
and incorporate new data.

        The  Agency  initiated a  significant
effort addressing dioxin risk, and on Septem-
ber 13, 1994, released for public review and
comment a 2,400 page draft reassessment of
the toxicity of and exposure to dioxin (see 59
FR 46980).  The development of this "public
review draft" involved outside scientists  as
principal authors of several chapters,  several
public meetings to take  comment on the
Agency's plans and progress, and the publica-
tion of earlier drafts for public comment and
review.  The draft reassessment broadened its
focus beyond 2,3,7,8-TCDD to include other
dioxins, furans, and coplanar PCBs on the
basis of equivalence of response in terms of
Ah-receptor binding.  Such receptor binding
was considered an essential, if not sole deter-
minant. The document was based on informa-
tion currently available to EPA regarding the
toxicity, sources, pathways of release to the
environment, and the levels of these com-
pounds in the environment. It recognized that
these  compounds vary in potency and used
toxicity equivalents based on  experimental
data to develop overall risk estimates.

        In December 1994, the EPA Office of
Research and Development (ORD) requested
that the SAB review the reassessment docu-
ment, and submitted a draft Charge addressing
some 40 issues.  Following discussions involv-
ing ORD and SAB  staff,  and the Co-Chairs
appointed by the SAB Executive Committee to
lead the review, a final Charge with 43 issues
(see Section  2.2  following) was adopted.
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                                                                           PAGE   7
       The SAB Dioxin Reassessment Review
Committee (DRRC) was developed by build-
ing on the SAB's Environmental Health and
Indoor Air Quality/Total  Human Exposure
Committees, and adding (following an exten-
sive  review and recruitment  process)  addi-
tional  Consultants  to fill gaps  in  needed
expertise and to add depth in key scientific
areas.   In addition to the Co-Chairs,  37
scientists were appointed to the Committee.
The DDRC met on  May 15-16,  1995  in
Herndon, Virginia  to  hear briefings by EPA
staff  and comments  by  Members  of the
public, and to discuss the  relevant issues  of
the Charge.  Following the public meeting,
the present report  was developed  through a
series of mail reviews of  successive  drafts,
continuing until consensus was reached,  or
statements of both majority and minority
viewpoints were incorporated.

2.2  Charge

       Sections 2.2.1  and 2.2.2 following
display the detailed Charge for the review.
The Charge consists of background material
supplied  by  EPA  Office  of  Research  and
Development (ORD)  staff, and the  specific
questions agreed upon following discussions
between   the  Committee   Co-Chairs, SAB
staff,  and ORD staff.  The questions  are
displayed in italic  text to  differentiate them
from the background material.

2.2.1  Exposure Document Charge

OVERALL SCIENTIFIC  FOUNDA-
TIONS OF THE REASSESSMENT
DOCUMENT

(Question  1)  Overarching  the specific  issues
addressed  below and not withstanding any specific
finding of the Committee(s), do the available data
and the analyses of these data, as presented in the
draft, adequately support the major conclusions of
the reassessment documents?

        The exposure document was devel-
oped by EPA's Exposure Assessment Group
with contract support from Versar, Inc.  The
effort began in 1992 and has included several
internal review cycles and one external review.
The primary objectives of the exposure reas-
sessment document are to:

  a)     identify the sources that release
        dioxin-like compounds to the envi-
        ronment;
  b)     summarize data on the levels of
        these compounds in food and envi-
        ronmental media;
  c)     summarize data on human body
        burdens;
  d)     estimate background exposure lev-
        els; and
  e)     provide procedures for estimating
        human exposure as a result of site-
        specific releases

        The  key findings of  the exposure
document include the following:  The princi-
pal pathway by which people are exposed to
dioxin-like compounds is through the diet,
with the consumption of animal products
contributing over 90%  of  the  average daily
intake. It is hypothesized  that the principal
mechanism  by which dioxin-like compounds
enter  the terrestrial food chain is via atmo-
spheric transport and deposition.   Current
levels of these compounds in the environment
are principally caused by anthropogenic activi-
ties.   The  EPA reassessment  document is
based on an extensive literature review which
is complete  through 1993 and includes a
REASSESSING DIOXIN

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PAGE  8
number of 1994 references. Over 1000 refer-
ences were used.

       EPA is seeking comment from the SAB
in each  of the above five  areas,  as well  as
posing this general question: (Question 2) Are
currently  available  models  and  approaches for
estimating and apportioning the impacts of various
sources adequate for  this purpose - i.e., addressing
the specific issue areas enumerated below? Have the
best extant approaches been employed?

SOURCES

1.   An  inventory  of  CDD/F (chlorinated
dibenzo-dioxins and furans) emissions to land,
air, and  water is presented in the document.
In general, the inventory is based  on proce-
dures and supporting  data comparable  to
similar inventories conducted by a number of
European countries. A qualitative uncertainty
classification  (low,  medium,  or  high)  was
given to  each estimate, and a range of uncer-
tainty around each estimate was  assumed.
(Question 3) Does the Committee recommend any
changes to this inventory?

2.  The document concludes that the  contri-
bution of historical sources ("reservoirs")  to
current exposures  is unknown.  (Question  4)
Can the  Committee suggest new ways  or  identify
new data to evaluate further the importance of these
sources?

3.  It is unknown whether local or distant
sources contribute most to food levels at a
particular location. (Question 5) Can the Com-
mittee suggest  new ways or identify new data  to
evaluate further this  issue?

4.  A number of investigators from  other
countries have evaluated  the  possible exis-
tence  of unknown  sources  by  comparing
estimates of emissions to estimates of deposi-
tion. Such an analysis is provided for the U.S.
in this document. It concludes that too much
uncertainty exists in both the emission and
deposition estimates to make any firm judg-
ments regarding unknown sources by compari-
son of these two estimates.  (Question 6) Does
the Committee agree with this position?

FOOD AND MEDIA LEVELS

1. The uncertainty in the estimates of CDD/F
levels in U.S. is difficult to characterize. The
document highlights the  limited number of
samples used to derive averages and presents
standard deviations (where possible to derive).
Also, an  analysis is  presented  showing the
impact  of  deriving  averages   assuming
nondetects  equal   zero   versus   assuming
nondetects equal half of the detection limit.
Finally, comparisons are made  to European
studies.  (Question 7) Has this uncertainty, due
both  to the possible varied quality of the data and
the  limited number of samples,  been adequately
emphasized and characterized?

2.  Some reviewers  have suggested  that the
current U.S. food data should not be used to
make preliminary estimates  of background
levels,  even with the  caveats.  (Question 8)
Does the Committee agree  with  the document's
approach?

3.  The document proposes  the hypothesis
that the air-to-plant-to-animal pathway is the
primary way that the food chain is impacted.
(Question 9) Does the Committee concur with the
rationale used to develop this hypothesis?  Can the
Committee offer further elaborations on the mecha-
nisms which result in food chain impacts?
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                                                                                 PAGE  9
4. The document concludes that environmen-
tal levels appear to be primarily a product of
anthropogenic activities.   This is based on
trends  seen in sediment data, which show a
rise in  the  concentration of dioxin-like  com-
pounds beginning in the first half of the 20th
Century. Only low levels have been found in
ancient tissue remains.  (Question 10) Does the
Committee agree with the conclusion that environ-
mental  levels are primarily a product of human
activities in the 20th Century?

HUMAN BODY BURDENS

Human body burden data are presented from
several studies.  Although some of these stud-
ies are quite large (e.g., NHATS (National
Human Adipose  Tissue  Survey)  collected
samples from over 800 individuals), they may
not be statistically representative of the entire
U. S. population.   (Question  11) Is  this an
important source   of  uncertainty for  projecting
background body burdens?

BACKGROUND EXPOSURES

Background exposures are  estimated  in two
ways:  a) using levels  in food and standard
consumption estimates; and b) by back-calcu-
lating from body burden data. (Question 12)
The Committee is asked to comment on the appro-
priateness of these calculations.

SITE-SPECIFIC ASSESSMENT PROCE-
DURES

1. The food chain and other fate models used
in this document are relatively simple, steady-
state approaches.  They generally use  parti-
tioning techniques to  model media transfers
without consideration of the  mass  of the
compartments.    Accordingly,  mass  balance
 violations  are  theoretically  possible.   The
 general issues associated with these models
 can be grouped into three areas:

 (Question 13) Is the steady-state assumption valid?
 Using steady state models is felt to be justified based
 on  the assumption  that the release rates of dioxin-
 like compounds are relatively constant and tenden-
 cies for these compounds to persist in the environ-
 ment.  The additional benefit of such models is that
 they tend to be less  data intensive, and information
 was found for all parameters for the dioxin-like
 compounds.   Does the Committee generally agree
 with the use of simple, steady state approaches for
 modeling the fate-and-transport of  dioxin-like
 compounds? Does the Committee have comments on
 specific models or model parameters?

 (Question 14) Is the lack of an  explicit mass-bal-
 ance derivation of the models an important concern?
 The current  models  have the advantage of being
 relatively easy to use. For example, bioaccumulation
factors, which are multiplied by media concentration
 to estimate tissue concentrations (plants, animals),
 tend to be simple to  use.  Limited testing suggests
 that mass balance violations are unlikely as long as
 reasonable parameter values  are used.   Also, the
 general recommendation is included to conduct mass
 balance checks after modeling.  Is this sufficient or
 are more complex approaches needed?

 (Question  15) Have the models been  sufficiently
 validated? Most aspects of these models were not
 derived on the basis of theory.  Rather,  they gener-
 ally use approaches and parameter values that were
 derived from  field  or  laboratory  observations.
 Chapter 7 of Volume HI details several exercises
 that are meant to address the validity of the models
 used in these procedures.  Some of these exercises are
 the description and application of alternate model-
 ing approaches  compared with  the  approaches
 selected for the assessment.  Additionally compari-
REASSESSING DIOXIN

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 PAGE   10
 sons were made of model predictions to field mea-
 surements/or the effluent discharge model, air-to-
 beef food chain model, and others. Although model
 validation is generally an ongoing concern, can the
 Committee make any statements as to the extent and
 merit of the validation exercises presented in Chapter
 7?  Can  they make suggestions about farther exer-
 cises which could be done? Can the Committee make
 statements as to the proper use of these models in
 light of the validation work presented in Chapter 7?

 2. In addition to the general issues discussed
 above, three more  specific fate model issues
 were highlighted:

 (Question 16) Does significant photolysis occur
 during atmospheric transport of dioxin-like com-
 pounds?   If so, how does the  toxic equivalency
 (TEQ) of the mixture change? Some evidence exists
 that rapid photolysis can  occur when  these com-
 pounds are present in  a vapor phase.   Testing,
 however, has not been conducted under true atmo-
 spheric conditions.  The degradation products have
 not been identified.  The possibility exists that higher
 chlorinated dioxins andfurans will yield more toxic
 lower chlorinated dioxins andfurans.  Given the lack
 of tests and unknowns about changes in TEQs, the
 document recommends  assuming no degradation
 during atmospheric transport.

 (Question 17) Have  the air-to-plant transfer
 coefficients been appropriately estimated?  These
 transfer factors are critical components of the dioxin
food chain model.   The document concludes  that
 transfers  of dioxins in  the vapor phase dominate
 above ground vegetative concentrations, particularly
feeds  of  livestock.   The transfer coefficients were
 derived from laboratory studies on the transfer of
 2,3,7,8-TCDD vapors onto grass leaves and other
 vegetation.  Questions remain, however, regarding
 extrapolation to other congeners, extrapolation to
other vegetation, effects of photolysis, wash-off rates
of deposited contaminants, etc.

(Question 18) Has the vapor/particle partitioning
been appropriately estimated?  The document re-
views adsorption theory and provides a procedure to
estimate  the degree  that dioxin-like  compounds
partition between the vapor and particle phases in
the ambient air.  The controlling factors are: molecu-
lar weight, chemical-specific vapor pressure, ambient
temperature, and concentration of total suspended
particulate.  Does  the Committee recommend any
changes to this approach?

3.   The procedures presented in Volume III
are  specifically  designed   for  assessing the
incremental impacts from specific  sources.
Two aspects of  such assessments were not
addressed in detail:

The general advice provided on considering
background exposures  when evaluating the
impacts from a specific site is that incremental
impacts can be  compared to national back-
ground  estimates for media  concentrations
and exposures (as  presented in Volume II).
No additional details or suggestions are pro-
vided. (Question 19)  Does the Committee agree
with this approach? Does  the Committee have any
specific recommendations?

The general advice provided on evaluating
impacts from multiple sources of release is that
point sources can be modeled individually and
impacts summed at points of interest, but no
additional details or suggestions are provided.
(Question 20) Does the Committee agree with this
approach, or would the  Panel recommend  that
multiple  sources be  more explicitly discussed  in
Volume III? And if so, does the Panel have any
specific recommendations for such inclusions?
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                                                                            PAGE  11
  2.2.2  Health Document Charge

       Chlorinated   dibenzo-p-dioxins  and
related compounds (commonly known simply
as dioxins)  are  contaminants present in a
variety of environmental media.  These com-
pounds are extremely potent in  producing a
variety  of effects in experimental  animals
based  on traditional toxicology studies at
levels hundreds or thousands of times lower
than most other chemicals of environmental
interest.  In addition, human studies demon-
strate  that  exposure to dioxin  and  related
compounds is associated with subtle biochem-
ical  and  biological  changes whose clinical
significance  is  as yet  unknown, and with
chloracne, a serious skin condition associated
with high level exposure to these and similar
organic chemicals. Laboratory studies suggest
the probability that exposure to dioxin-like
compounds  may be associated with other
serious health effects including cancer.  Hu-
man data, while often limited in their ability
to answer questions of  hazard and risk,  are
generally  consistent with the observations in
animals.  Whether the adverse effects noted
above are expressed in humans, or are detect-
able in human population studies, is depen-
dent on the dose absorbed and  the  intrinsic
sensitivity of humans  to  these  compounds.
Recent laboratory studies have provided new
insights into the mechanisms involved in the
impact of dioxins on various cells and tissues
and, ultimately,  on toxicity.  Dioxins have
been demonstrated to be  potent modulators
of cellular growth and differentiation, particu-
larly in epithelial tissues. These data, together
with the collective body of information from
animal and human studies, when coupled with
assumptions and inferences regarding extrapo-
lation from experimental animals to humans
 and from high  doses to low doses, allow a
 characterization of dioxin hazards.

         EPA is seeking comment from the
 SAB  in the following  areas  of  the  Health
 Assessment document:

 OVERALL  SCIENTIFIC FOUNDATIONS
 OF THE REASSESSMENT DOCUMENTS

 (Question 1) Overarching the specific issues ad-
 dressed below and not withstanding any specific
finding of the Committee(s), do the available data
 and the analyses of these data, as presented in this
 draft, adequately support the major conclusions of
 the reassessment documents?

 DISPOSITION/PHARMACOKINETICS

 The  disposition  and pharmacokinetics  of
 2,3,7,8-TCDD and  related compounds have
 been investigated in several species and under
 various exposure conditions.  These data and
 models  derived  from them  are  critical  in
 understanding the sequelae of human expo-
 sure.   Data related  to disposition and phar-
 macokinetics  of  dioxin and  related  com-
 pounds  and  efforts  to  develop  models  to
 further  understand  tissue  dosimetry  are
 described  in detail  in  Chapter 1  of the
 Health Assessment document:

         An   understanding  of the relation-
 ship between exposure and dose is an impor-
 tant aspect of ai\ adequare characterization of
 risk.  The data base relating to  this issue is
 extensive for 2,3,7,8-TCDD but is lacking for
 many of the related compounds,  (Question 2)
 Does  the document adequately characterize  the
 strengths and  weaknesses of the  data base and
 draw  appropriate  inferences for this group  of
 compounds?
REASSESSING DIOXIN

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PAGE  12
       The  evaluation of available data and
the  development of  physiologically based
models has  led to a better understanding of
the  disposition  and  pharmacokinetics  of
dioxin and related compounds than for most
other environmental chemicals. (Question 3)
Has this understanding been well integrated  into
other aspects of the assessment such  as route of
exposure, toxicity equivalence, dose to the fetus,  etc?

       This assessment relies extensively on
estimates of body burden that are a function
of the uptake, distribution, metabolism, and
excretion of this complex mixture of structur-
ally related compounds. Estimates of half-life
in the body facilitate the understanding of
bioaccumulation as a function of intake over
a life-time, and of the impact of incremental
exposures on blood or tissue levels  both over
the short and long term.  (Question 4) Have
these issues been adequately dealt with in the health
assessment document?

       Body burden data allow some estima-
tion of historical body burdens to complement
effects analysis  in  human populations  pre-
sumed to have high exposures in earlier de-
cades.  (Question 5) Has the magnitude, implica-
tions and uncertainty of these back extrapolations
been adequately described?

MECHANISMS OF DIOXIN ACTION

Knowledge  of the  mechanisms   of dioxin
action may facilitate  the risk assessment
process by imposing bounds upon the assump-
tions  and models used to  describe  possible
responses to exposure to dioxin. In  this docu-
ment,  the relatively 'extensive database on
dioxin action has been reviewed, with empha-
sis on the contribution of the  specific cellular
receptor for dioxin  and related compounds
(the Ah receptor)  to the mechanism(s)  of
action.  Other reviews referenced in Chapter
2 provide additional background on the sub-
ject.  Discussion in this chapter focusses on
aspects of our understanding of mechanism(s)
of dioxin action that are particularly impor-
tant  in  understanding and characterizing
dioxin risk including:

 a)     the similarities at the biochemical
        level between humans and other
        animals with regard to receptor
        structure and function;
 b)     the relationship of receptor binding
        to toxic effects; and
 c)     the role that  the purported mecha-
        nism^) of action might contribute
        to the diversity of biological re-
        sponses seen  in animals and, to
        some extent,  in humans.

(Question 6) Has the Health Assessment provided
a useful summary and balanced perspective on these
issues? Is the advance  in knowledge of details of
early cellular events in response  to dioxin exposure
clearly distinguished from our paucity of knowledge
of the direct impact of these events on toxicity?

TOXIC EFFECTS OF DIOXIN

 General Issues

It  is clear from the evaluation of the toxico-
logic literature that dioxin and  related com-
pounds have the ability to  produce a wide
spectrum of responses  in animals and,  pre-
sumably,  in humans,  if the  dose is high
enough. Relatively few chronic effects related
to exposure  to dioxin-like compounds have
been observed in humans. The epidemiologic
data are limited due to a number of possible
factors: the absence of many, specific individ-
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                                                                              PAGE   13
ual measurements of dioxin exposure for the
general population; a limited number of cross-
sectional  and  prospective studies of  more
highly exposed populations; the limited ability
of epidemiologic studies to detect significant
differences 'between  exposed and relatively
unexposed populations when  the  outcomes
are relatively rare, the exposures are low, and
the population under study is small; and the
difficulty in quantifying the  impact of all
potentially confounding exposures.   Evalua-
tion of hazard and risk for dioxin and related
compounds must rely on a  weight-of-the-
evidence approach in which all available data
(animal and human) are  examined together.
This  process often requires extrapolation of
effects across various animal species, as well as
to humans.

       The  reliability of using animal data
to  estimate  human  hazard  and risk  has
often been  questioned  for  this class  of
compounds.  The document takes the posi-
tion that, although human data are limited,
evidence  suggests that  animal  models  are
appropriate for estimating human risk if all
available  data  are considered.  (Question 7)
Does the Committee agree?

       For  purposes of  the current  assess-
ment, unless  there are  data  to identify  a
particular species as  being representative of
humans   for  a  particular  effect,  average
humans can be reasonably assumed to be of
average sensitivity for various  effects, recog-
nizing that  individuals  in  the  population
might vary widely  in  their  sensitivity to
individual effects.   (Question 8)  Is  this  a
reasonable position to  take given the available
data? Is  the rationale for this assumption clearly
stated?
Chloracne

        Chloracne is the only clearly adverse
health  effect which is known to  occur in
dioxin  exposed  humans.    Recognition  of
chlofacne  has  been  associated  with  high
level exposure to these compounds,  and as
such, may  represent  a  biomarker  of expo-
sure.  (Question  9)  Does  the  Committee agree
with the position stated in the Health Assessment
that,  because  of  the wide  variability  of the
chloracnegenic response in humans and its varied
persistence,  the absence of  chloracne  is  not  a
reliable indicator of low exposure  to dioxin  and
related compounds?

Cancer

        While the data base from epidemio-
logic studies remains controversial, it is the
view of this reassessment that this body of
evidence supports the laboratory data indicat-
ing  that TCDD probably increases  cancer
mortality of several types.  The  Peer Panel
that met in September, 1993, to review an
earlier draft of the cancer epidemiology chap-
ter suggested that the epidemiology data alone
were still not adequate to implicate dioxin and
related compounds as "known" human carcin-
ogens but that the  results from the  human
studies  were largely consistent with observa-
tions from  laboratory studies  of dioxin-in-
duced cancer and,  therefore, should  not be
dismissed or ignored. Other scientists, includ-
ing those who attended the Peer Panel meet-
ing, felt either more  or less strongly about the
weight  of  the evidence  from  epidemiology
studies, representing the range of opinion that
still exists on the interpretation of the cancer
epidemiology studies. (Question 10) Does the
Health  Assessment document  adequately reflect
those views?  Have uncertainties in the epidemiology
REASSESSING DIOXIN

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PAGE   14
data base been  well characterized?  Would the
Committee care  to add its view to those already
stated in the document?

       An extensive data base on the carcino-
genicity of dioxin and related compounds in
laboratory studies exists and is described in
detail in Chapter 6. There  is adequate  evi-
dence that 2,3,7,8-TCDD is a carcinogen in
laboratory animals.  Few attempts have been
made  to demonstrate the carcinogenicity of
other  dioxin-like compounds.  Other than a
mixture   of  two  isomers  of  hexachloro-
dibenzodioxin (HCDDs) which produced liver
tumors in both sexes of rats  and mice (NTP,
1980), the more highly chlorinated CDDs and
CDFs have  not  been  studied  in  long-term
animal cancer bioassays. However, it is gener-
ally   recognized  that   these   compounds
bioaccumulate  and exhibit toxicities similar to
TCDD and  are,  therefore, also  likely to be
carcinogens (SAB, 1989).  (Question II)  Does
the Committee have any additional comments on
extending the inference of carcinogenicity of 2,3,7,8-
TCDD to the broader class of dioxin-like compounds
as defined in the assessment document?

       The   Health  Assessment document
describes dioxin  and related compounds as
complete carcinogens, based on their ability to
produce  tumors in all animals tested in the
absence of exogenous initiating agents.  At the
same time, it recognizes that these compounds
can be described operationally as potent pro-
moters of carcinogenicity without traditionally
defined genotoxic activity. (Question 12) Has
the document adequately characterized the carcino-
genic activity of these compounds so as to distinguish
between these descriptors?

       The   EPA's   1985  classification  of
2,3,7,8-TCDD as a "probable" human carcino-
gen under the Agency's risk assessment guid-
ance for carcinogens was based exclusively on
the adequacy of the animal  carcinogenicity
database. The current assessment character-
izes 2,3,7,8-TCDD and related compounds as
likely to be human carcinogens  under some
conditions of exposure,  and  re-affirms  the
classification of "probable" carcinogens  but
with greater confidence  than  in  1985.  In-
creased confidence is based on the total weight
of the evidence based on unequivocal animal
evidence, limited human evidence and mecha-
nistic evidence supporting biological plausibil-
ity. (Question 13) Does the Committee agree with
this characterization of the cancer hazard of dioxin
and related compounds? Given efforts underway to
revise the Agency's  Cancer Guidelines, should  this
class of compounds be assigned an alphanumeric
classification according to the 1986 Guidelines?  (A?
Bf?B2?)

Developmental Toxicity

        Since developmental toxicity follow-
ing exposure to TCDD-like congeners occurs
in fish, birds, and mammals,  it is  likely to
occur at some level in humans.  It is not cur-
rently possible to state exactly how or at what
levels humans in the population will respond
with  adverse impacts  on  development  or
reproductive function. Data analyzed in Chap-
ter 5 and Chapter 7 suggest,  however, that
adverse effects may be occurring at levels lower
than originally thought  to represent a  "no
observed  adverse  effect  level  (NOAEL)" in
animals.   Traditional toxicology studies had
led to the conclusion that the NOAEL was in
the range of intake values of 1 ng TEQ/kg/day.
(Question  14) Does  the  Committee agree that
current data  would suggest  that the NOAEL in
animals should be lower?
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                                                                              PAGE   15
I mmunotoxicity

       Evidence has accumulated to demon-
strate that the immune system is a target for
toxicity of TCDD and structurally  related
compounds. The evidence has derived from
numerous  studies in various animal species.
Animal    studies   suggest   that    some
immunotoxic responses may be evoked at very
low levels of dioxin exposure. Epidemiological
studies also provide conflicting evidence  for
the immunotoxicity of these compounds in
humans. Few changes in the immune system in
humans associated with dioxin body burdens
have  been detected when exposed humans
have been studied. Both  direct and indirect
(e.g.,  hormonally mediated)  impacts on the
immune system have been hypothesized to be
the basis of dioxin immunotoxicity. (Question
15) Has the significance of the human data been
adeauately characterized?  Are the clinical methods
which  have been applied  to immune junction in
humans exposed  to dioxin sufficiently sensitive to
detect immunotoxicity? Does the Health Assessment
provide sufficient discussion  of  the strengths and
weaknesses of the animal data on immune function
to support its conclusions regarding the potential for
immunotoxicity in humans exposed at or near back-
ground levels?

Other Effects

       A number  of other effects of dioxin and
related compounds  have been discussed in
some detail throughout the  chapters in this
assessment. While they serve to illustrate  the
wide range of effects produced by this class of
compounds, some may be specific to the spe-
cies in which they are measured and may have
limited relevance to the human situation.  On
the other hand, they may be indicative of the
fundamental level at which dioxin produces its
biological impact and may represent a contin-
uum of response expected from, these funda-
mental changes. While all may not be adverse
effects (some may be adaptive and of neutral
consequence), several effects have been noted
in human studies or in primates and have been
given special mention. (Question 16) Are there
other important effects that should be highlighted?

DOSE-RESPONSE

Development  of biologically-based  dose  re-
sponse models for dioxin and related  com-
pounds as a part of this reassessment has  led
to considerable  and valuable insights regard-
ing both  mechanisms of dioxin action and
dose response relationships for dioxin effects.
These are described in some detail in Chapter
8.  These efforts have  provided additional
perspectives on traditional methods such as
the linearized multistage (LMS) procedure for
estimating cancer potency or the uncertainty
factor approach for estimating levels below
which non-cancer effects are not likely to
occur. These methods have also provided a
biologically based rationale for what had been
primarily statistical approaches. The develop-
ment of models like those in Chapter 8 allows
for an iterative process of data development,
hypothesis testing, and model development.
These efforts have resulted in incorporation of
more of  the  available biological data into
models to predict human risk at low incre-
ments of exposure.   (Question  17) Does  the
Committee agree  with  the  approaches  to dose-re-
sponse that have  been  used in the Health Assess-
ment?   Given  the  evolving nature of this effort
should collaborative efforts to refine these techniaues
continue as a high priority?

        The U.S. EPA has frequently defined
a reference dose (RfD) for toxic chemicals to
                DIOXIN

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PAGE  16
represent a scientific  estimate  of  the  dose
below which no appreciable risk of non-cancer
effects is likely to occur  following chronic
exposures.  In the case of dioxin and related
compounds, calculation of an RfD  based on
human and animal data and including  stan-
dard uncertainty factors to account for spe-
cies differences and sensitive sub-populations
would result in reference intake  levels on the
order of 10-100  times  below  the  current
estimates of daily intake in the general popu-
lation.  For most compounds where RfDs are
applied,  the compounds are not persistent,
background exposures  are generally low, and
are not  taken  into account.   Dioxin  and
related   compounds  present  an  excellent
example  of a case where background levels in
the general  population  are likely  to  have
significance  for evaluation of  the relative
impact of incremental exposures associated
with a specific source.  Since RfDs refer to
the total chronic  dose  level,  the  Health
Assessment document takes the  position that
the use of the RfD in evaluating incremental
exposures in the face of a background intake
exceeding the RfD would be inappropriate.
(Question 18) Does the  Committee agree? Is the
rationale for this position clear?

      Observations described in this assess-
ment  suggest a continuum of  response to
exposure  to dioxin-like  chemicals.   By  a
continuum of response,  we suggest that as
dose increases the  probability of occurrence
of individual effects increases and the sever-
itv of collective effects increases.  This  con-
 ^
tinuum  provides a  basis for inferring a rela-
tionship  between  some  early events which
are not  necessarily considered to be adverse
effects with later  events which are adverse
effects.   Considerable uncertainty remains in
inferring how  these events are related, al-
though we know more about how dioxin-like
compounds may elicit effects than we know
about the mechanisms  of action for  most
chemicals.  This inference may be the  most
contentious of all and it is likely that a wide
range of  opinion  will  be provided by the
scientific community regarding the relation-
ship of these mechanistic observations  and
prediction of potential for adverse effects in
exposed  humans.   (Question  19)  Does the
Health Assessment document provide a balanced
perspective regarding the uncertainties embodied in
this inference?
TOXICITY EQUIVALENCE FAQ
TORS (TEFsl

The EPA  and  the  international  scientific
community have  agreed  that  the  use  of
toxicity  factors (TEFs)  to predict relative
toxicities of mixtures of this class  of com-
pounds has an  empirical  basis,  is theoreti-
cally  sound, and,  in the absence of more
complete data sets on the toxicity of individ-
ual members of this  class, is a useful proce-
dure!  This  is not to say that the use of TEFs
is  a'tlertain procedure. Since 1986 when the
firstc "Agency-wide  consensus on  the use of
TEFsk was  published, additional refinements
to the data bases  and to the use of TEFs
have occurred.  Published revisions in accord
with  international  agreement appeared  in
1989.   In the course of this reassessment,
critical data were  collected and agreement
was  reached regarding the contribution of
dioxin-like PCBs to overall TEQs.  Additional
validation  of the TEQ concept in predicting
effects of this class  of compounds on wildlife
species lends further support to the use of
this approach.   This  relatively simple, addi-
tive  approach  does  not  take  into  account
interactions between dioxin-like  compounds
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                                                                             PAGE   17
and  other chemical exposures.  (Question  20)
Does the Committee agree with the EPA's use of
TEFs in the Health Assessment document? Have
the uncertainties and the assumptions intrinsic to
the use of TEFs been  clearly described?  Should
EPA consider presenting the assessment results in
an alternative manner?

LABORATORY ANIMALS/ HUMAN
RESPONSE

Based  on our knowledge of the biochemical
and biological similarities between laboratory
animals  and humans, our understanding of
some of the fundamental impacts of this class
of compounds on biological  systems,  and
comparable responses from animal and  hu-
man studies both in vitro  and in vivo, EPA
has made the decision to use laboratory  ani-
mal  data to contribute to weight-of- the-evi-
dence conclusions on human hazard and risk.
(Question 21) Does the Committee agree that this
decision is reasonable?

       Humans do not appear to be an un-
usual responder  for dioxin effects;  that is,
humans  do not,  on  average,  appear to be
 either refractory  to or exquisitely sensitive to
the effects of dioxin-like compounds. While
positive human data are preferable for ascrib-
ing hazard or risk, the lack of adequate hu-
man data to demonstrate causality for many
 suspected dioxin effects  is assumed not to
negate the findings  from laboratory animal
and  in  vitro  studies.     Although  some
scientistsmay disagree, in our estimation, the
database on dioxin  and related compounds
regarding a wide  range of  responses across
animal species is one of the most comprehen-
sive among all  environmental chemicals.  The
fundamental   understanding of mechanisms
of dioxin action provides a unifying theory for
the mechanisms for observed effects in labo-
ratory animals and humans, and for using  a
weight-of-the-evidence  apptcoh considering
a                    1                    1
relevant data to infer the human health im-
pacts  of  dioxin  and  related  compounds.
(Question 22) Have the strengths and weaknesses
of this position been well articulated?

OVERALL CONCLUSIONS

 The final chapter of the Health Assessment
document integrates  information on exposure
and effects relating  to the impact of dioxin
and related compounds on human health. It
also contains overall conclusions on this issue.
As such, it represents a risk characterization.
(Question 23) Does this chapter adeauately charac-
terize the database, assumptions, and uncertainties
relating to the potential health effects of dioxin and
related compounds? Does it provide the information
in enough of a public health context to be under-
standable to decision-makers?

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PAGE  18
             3.  DETAILED FINDINGS-EXPOSURE DOCUMENT
3.1  Sources

3.1.1  Estimating and Apportioning
       Sources (Charge Question 2)

       The  approach  for  estimating  and
apportioning the  relative contributions  of.
various types of sources is to multiply average
emission factors for each source type by the
mass flow (i.e., the amount of combustion) in
each category to estimate the total emissions
of dioxin and dioxin-like compounds5 to the
environment for each source category,  then
sum the totals and examine the relative contri-
butions of each type of source.

       It  should  not be assumed  that the
fractional contributions of various  types  of
combustion  sources  to total  emissions are

   ... it is thus not scientifically
      valid to infer from such a
   comparison that there are any
    missing sources of dioxins.

identical or  similar to their contributions  to
human exposures  for the population in gen-
eral.   This  distinction should be  explicitly
stated.  It is quite possible that the major
sources of dioxin  in food may not  be  those
that represent the largest fractions of  total
emissions in the U.S.  The geographic loca-
tions of sources relative to the farms from
which much of the beef, pork, milk, and fish
come, is important to consider. That is, the
farm lands which produce much of our food
may not be necessarily located near the major
sources of dioxin and related compounds.

        Total estimated dioxin-like emissions
for the U.S. have been compared to an esti-
mate  of the total amount of dioxin  that is
deposited to the surface of the U.S. based on
available deposition factors. The ranges of the
two estimates overlap, giving some suggestion
of a mass balance for the U.S.  as a  whole.
Although such mass balance comparisons have
been  published in the literature, there is a
significant scientific problem with doing this
very simple comparison on a continental or
regional  scale  without  doing  atmospheric
dispersion  and deposition modeling  of the
emissions.    Specifically,  the   atmospheric
lifetime  of the  accumulation mode particles
(0.1 to about 2-3 Aim), in which  much of the
dioxins and dioxin-like compounds are found,
is on the order of many days. Thus,  a large
proportion  of  the particles with dioxin-like
compounds that are emitted in the  eastern
U.S. are likely to be deposited in the Atlantic
Ocean, Canada, Europe, or even the  Arctic,
depending upon source locations  and weather
patterns.  Given the long atmospheric half-life
of the particulate dioxin-like compounds, a
global or hemispheric mass balance would be
necessary.  In  order to  do a  scientifically
acceptable mass balance comparison of emis-
sions and deposition for the U.S., deposition
of emitted dioxins must be estimated using
atmospheric dispersion and deposition model-
ing, and the  deposition  estimates from the
emissions then compared to measured deposi-
tion data.  In addition, more representative
measured deposition data would be needed.
were included.
        For the source estimation, only dioxins and furans
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                                                                             PAGE  19
       The Committee recommends that this
section of the report be  modified so that
this simple  direct mass balance comparison
is not provided, and the scientific  reason for
not doing so be explained.  The Committee
agrees with EPA's statement (Volume II, p.
3-166) that it  is thus not  scientifically valid
to infer from such a comparison  that there
are any missing sources of dioxins.

3.1.2  Source Inventory  (Charge Ques-
        tion 3)

        The dioxin emission inventory has identi-
fied the major known sources and has, in general,
made a reasonable estimate of the total emissions
from  each source  category.   However, some
revisions should be made.

        The  incineration  of medical waste
was  estimated to be  the  largest source of
dioxin  emissions.  After this  estimate  was
made,  new  information  became available
that  indicates  that current emissions  from
medical waste  incineration might be signifi-
cantly lower than initially estimated by EPA.
The  EPA should  review  these  (and  any
other) new data that becomes available, and
revise the estimates as appropriate.   The
emission estimates for industrial and residen-
tial  wood combustion  should also be  re-
viewed and revised, as well as the estimates
for dioxin emissions from the combustion of
diesel fuel.

        The Agency should assess the time-
frame for the emission inventory.  Also, EPA
needs  to  evaluate  more thoroughly  the
emissions data  and define more carefully the
width of the uncertainty  range based  upon
engineering assessments and data availabil-
ity.
        Due to the uncertainties in dioxin
emissions  data, and the variability in emis-
sions, there is significant uncertainty in the
emission estimates. The EPA has estimated an
uncertainty range of either a factor of five or
a factor of ten for each dioxin source category,
based primarily on the uncertainty  in the
estimated emission factors.  The  Committee
thinks that the uncertainty in emissions has
been underestimated in some cases. In  addi-
tion,  uncertainty  classifications  should  at-
tempt to build upon the classification systems
already used in existing EPA emissions factors
databases.

         There  appear to be some sources
identified in  international inventories or by
commentors  which  were not considered or
were not  assessed in the EPA Reassessment
Document. Some discussion of these sources
is important to the completeness of the report
even if they are later determined to be negligi-
ble sources of CDD/CDF emissions.

3.1.3   Dioxin Reservoirs (Charge Ques-
        tion 4)

        The  reassessment  document  indi-
cated that in addition to exposures  from
sources that are currently emitting CDD/CDF,
it is possible that CDD/CDF from historic
reservoir sources  are being re-introduced to
exposure media.  The  assessment conducted
on this issue  (section  3.7 of the EPA docu-
ment) was a simple evaluation of the relative
importance of one year's emissions  from
currently   emitting  sources to  those  from
preexisting reservoirs. This simple assessment,
based  upon  an   assumption of first-order
dissipation rates and an assumed half-life of
dioxin in  the reservoir, indicated the  large
potential  size of the   CDD/CDF reservoirs
 REASSESSING DIOXIN

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PAGE  20
relative to annual deposition rates.  Given
that heretofore, the presence of CDD/CDF in
subsurface lake and ocean  sediments had
generally  been considered to be innocuous
deposits, no  attempt was made to quantify
further the contribution of the reservoirs to
human exposure.

       The Committee concluded that the poten-
tial contributions from reservoirs might indeed be
important  and should be evaluated.  It is  im-
portant to be able  to evaluate  the relative
contributions  of  reservoirs  and  currently
emitting sources, particularly in light of the
fact that  emitting sources appear to  be in
decline.   Thus  the  relative contribution of
these  reservoir  sources  may  become even
more important.  A limiting case which may
be particularly informative is an assessment
of  exposure  in  the absence  of currently
emitting sources.   Some of the reservoirs,
such  as sediments,  may act as a relatively
minor continuous source from surface bio-
logical  processes,  but become much more
significant  during  major   storm  events,
cleanup, or  navigational dredging.   Other
reservoirs  may be  relatively active and their
continuing contribution  to exposure  should
be  assessed  in order  to account  for all
sources of exposure.

     It is important to be able to
        evaluate the relative
  contributions of reservoirs and
     currently emitting sources,
   particularly in light of the fact
  that emitting sources appear to
	be in  decline.	
       The Committee suggests a  technique
to evaluate the potential importance of reser-
voir material to exposure. EPA could evaluate
the plausibility of different reservoir materials
re-entering exposure scenarios  through  the
use of engineering estimates and limited case
analysis. Although this analysis would not be
quantitative since the actual extent of reser-
voirs materials is not fully known, the analysis
would provide some assessment of the impor-
tance of particular reservoirs and  exposure
scenarios.

3.1.4   Local/Distant   Contributions  to
        Dioxin Levels  in  Food   (Charge
        Question 5)

        The question of the relative contribu-
tion of local and distant sources to food levels
at a site is a difficult one to answer experimen-
tally and would probably  require extensive
studies  at many sites.  Furthermore, the  an-
swer could well be different in different parts
of the U.S.

        In the future, better data and new or
revised models may make such activities more
feasible, and  lead to a full understanding of
the movement of contaminants from emission
sources  to food pathways.

        When better ouality data are available,
the Agency should consider using geographic infor-
mation systems (GIS) for their analysis.  With
such systems, the geographic distributions of dioxin
emissions and food (beef, pork, chicken, milk) could
be mapped and compared. Although not quanti-
tative, this examination is  likely to produce
some insights and testable hypotheses.  Once
the more current measurements of dioxins in
food become  available,  these  can  also be
mapped and quantitative questions can be
asked and tested statistically regarding  the
probable influences  of local, regional, and
distant sources.
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                                                                            PAGE  21
3.1.5  Uncertainty in Deposition Esti-
       mates  (Charge Question 6)

       Various research groups have  tried to
balance the input of dioxins into the atmo-
sphere with their outputfrom the atmosphere.
The input calculations are based on source
inventories, done on a national, region, and
international scale. The output estimates are
based  on  calculations  and measurements of
fluxes  from the atmosphere.  Most of these
data (summarized in Volume II, pages 3-4, 3-
5, and 3-166  to 3-168) indicate a large dis-
crepancy between deposition from the atmo-
sphere and inputs into the atmosphere. This
discrepancy indicates that 10-50 times more
dioxins are being deposited from the atmo-
sphere than are being emitted into the atmo-
sphere. However, the simple mass  balance
comparison of the estimates of emissions and
deposition fails to take  into  account  the
atmospheric lifetimes  of accumulation  mode
particles and their long range transport in the
atmosphere. Thus a certain (unknown) frac-
tion of the dioxins emitted within the conti-
nental United  States are certainly transported
by the atmosphere and deposited beyond the
borders.  In addition,  dioxin deposition can
not be expected to be geographically uniform;
in consequence, this very simple mass balance
comparison cannot be used to infer unknown
sources.

       There  is another  technical  problem
with this comparison.  These deposition rate
estimates are based on direct measurements of
wet  and dry deposition by several research
groups. However,  these measurements have
been converted to TEQ values in order to
compare them to emission estimates.  This
conversion is justified by the overall  strategy
of the  dioxin reassessment;  however, it is
 strictly correct only when the ratios of the
 various congeners is the same in the various
 samples. To be technically rigorous, the deposition
 calculations and emission estimates should be based
 on  individual  congeners and  isomers, perhaps
focusing on a few of the most abundant compounds.

         The Committee, however, agrees with
 EPA that there is  much value in an evaluation
 of the mass balance comparison of emissions
 and deposition. Such a comparison (which we
 are pleased to learn from EPA staff is now
 underway  by the  Agency), however,  must
 involve  modeled  estimates  of  dispersion,
 transport, and finally deposition from sources
 within  a  given  region.     The  modeled
 emissions-based estimate of deposition could
 then be compared to measured deposition for
 the region.

         Improved  measurements  of deposi-
 tion fluxes for the  U.S.  is  a fruitful area for
 investigation.   Most published  studies  have
 taken  samples in highly industrialized and
 urbanized regions of the world.  It is risky to
 extrapolate these measurements to the entire
 United States. A  strategy in which deposition
 fluxes  are  calculated  from  geographically
 diverse samples taken throughout the United
 States could  lead  to  a more accurate and
 precise estimate of the total deposition rate to
 the United States.

 3.2      Food and Media Levels

 3.2.1    Problems in Estimating  CDD/F
         Levels (Charge Question 7)

         A very limited number of samples are
 available to make  the preliminary estimates of
 CDD/CDF in air, water, soil, and  food prod-
 ucts.   Such estimates are appropriate and
REASSESSING DIOXIN

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PAGE  22
useful, and  EPA has tried to provide some
idea of the uncertainties and variability in the
data.   However, there are some refinements
and additions that can and should be made in
revising the report.

a)     The  reported sample  analyses were
       performed  at different laboratories,
       using different methods of analysis,
       and the samples were not all collected
       and analyzed in the same time period.
       Thus,  there  are  some  underlying
       sources of uncertainty and variability
       which are not adequately represented
       by using simple statistics (mean, stan-
       dard deviation).  For example, if envi-
       ronmental levels are declining, as indi-
       cated by sediment samples,  the sam-
       ples may have a time variability which
       the reader might not recognize. Some
       cautions on these aspects of uncertain-
       ties should be added to the report.

b)     It is  recommended that the reported
       data be examined with respect to the
       number of significant figures used and
       that  these be reduced as appropriate.
       For example, the daily intake  should
       not be reported as 119 pg/day TEQ
       but as being on the order of 100.  By
       handling the results this way, it be-
       comes clear that there are not really
       significant  differences  between   the
       estimates for the U.S. and other coun-
       tries  given the  uncertainties  in  the
       estimates.

c)     Although the reassessment document
       makes no unwarranted  claims about
       the accuracy of the food data for expo-
       sure  assessment, it is  important  that
       policy makers be fully  aware  of the
        limitations of the use of the results in
        the policy arena.  For example, the
        Committee  does  not believe  that
        these data are appropriate for trends
        analysis of body burdens or for geo-
        graphical or  demographic trends in
        exposure. Nor are the currently avail-
        able data sufficient to  characterize
        the variability  of exposures in the
        U.S.  population.  In the summary
        volume, it would be very helpful to
        policy makers to explicitly point out
        these kinds of limitations and to note
        that the exposure estimate is only an
        estimate  of the central  tendency of
        exposures within the U.S.

        In the future, as better measured data
become available, the data analyses should
include use of probability techniques, not just
simple statistics.

3.2.2   Use of U.S. Food Data (Charge
        Question 8)

        U.S. food data for the estimation of back-
ground exposure to dioxin-like compounds is presently
inadequate, but the EPA is commended for its major
effort to obtain new data, on which the Commit-
tee was briefed, but did not specifically review.
Shortcomings aside, the available information
from  the  U.S. along with that from  other
countries indicates a central trend or mean for
background food exposure of about 100 pg per
day. A very recent food study effort by EPA
(with contributions from the U.S. Department
of Agriculture and the Food and Drug Admin-
istration) has generated significant data on the
TEQ content  of beef (Winters et al, 1994).
The new value is considerably (3 to 4 times)
lower than that used  in the document.  This
would suggest that the overall daily intake of
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                                                                            PAGE  23
PCDD/PCDF may be less than 100 pg. Addi-
tional data gathering  is now in progress for
milk samples, and is planned for pork, poultry,
eggs, and vegetable oils.

       Current information  on the variation
or distribution of this value within the U.S. is
imprecise, however.  The sampling part of the
EPA program  is quite strong as it is statisti-
cally planned,  and the distribution within the
U.S. is being  studied, as well as production
within various  regions.   From this initial
undertaking, it appears that sources of food
for much of the U.S. are widely distributed
geographically, and  that exposure is national
in scope  with only limited  regional differ-
ences.

       The  Committee  was  of the  opinion
that, in the cases where analyses showed non-
detectable numbers (ND), the best estimate of
the true value, with currently available infor-
mation and methodology, was to use one-half
the limit of detection.  The effect of using this
approach on measures of central tendency of
a distribution depends, however, on the actual
detection limit (which is a function of sample
properties and analytical procedures) and the
proportion of values in the distribution at or
below the detection  limit.  For example, if the
detection limit is relatively high, and a large
proportion of the values in the distribution
are at or below the limit of detection, then
using one-half the detection  limit will proba-
bly overestimate measures  of  central  ten-
dency.   The Committee suggests  that EPA
explore  alternative  statistical  approaches to
handling ND values in the future, particularly
the  well-established  maximum  likelihood
method.  This approach requires only that the
parametric form of the underlying distribution
be known.
        The food study program underway by
EPA shows a good detection limit for TCDD
(0.05 ppt on a fat basis or about 0.01 ppt on
a whole weight basis) but significantly higher
values (0.5 ppt fat or 2.5 ppt whole weight)
for the penta-, hexa-, and hepta- congeners.
For example the food data of Schecter et al.
(1993) have  been  criticized for its lack of
controlled sampling but the detection limits of
the analytical method are lower than those of
EPA.  In order to minimize the variation in
estimation of the PCDD/PCDF  content of
foods by this uncertainty in detectability, EPA
should try to improve their analytical detec-
tion limits for the penta-,  and hexa- congeners
(those for the hepta-  and octa-  are  not as
important since  they contribute less to the
TEQ).  In this  activity, there is a tradeoff
between reliability of analytical levels and the
detection limit.   In general, the closer  one
approaches the detection limit of a method,
the less certain one becomes of the value, and
the further one is removed from the detection
limit, (usually) greater confidence is given to
the analytical value. The Committee believes
that EPA, in measuring PCDD/PCDF concen-
trations in foods, should take the necessary
steps to obtain detection limits on the samples
as low  as technically feasible.  Such an ap-
proach will minimize the uncertainty engen-
dered by using one-half of the detection  limit
for ND results.   The Committee  considers
that EPA is putting (correctly) more weight on
the latter. It is also desirable that EPA report
data on both a whole-weight and fat basis to
compare to other studies.

        A number of public comments related
to a paper presented at Dioxin 93 (Welge et
al., 1993) showing that the PCDD/F content
of blood was the same  for vegetarians  and
non-vegetarians.  The Committee  is of the
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PAGE  24
opinion that the above study is inconclusive.
The individuals classified as vegetarians fol-
lowed such a diet  between  5  and 10 years
(median values). Classification as a vegetarian
was based on non-consumption of both meat
and fish.  The diet was not verified  by any
means, and the study  did not address con-
sumption of milk, dairy products, and eggs --
all known food sources of PCDD/PCDFs.

3.2.3  Air/Plant/Animal Pathway and
       Other Food Chain Impacts
       (Charge Question 9)

       Given the existing data,  it  is probably
premature to  conclude  that  the  air-to-plant-to-
animal pathway6 is the primary way the entire food
chain is impacted. Nonetheless, this is a reasonable
hypothesis, one  that  is consistent both with  the
extant data  and existing models.  Based on the
analysis of the  existing  and very limited food
measurement data, EPA has focused on the
air-to-plant-to-animal exposure pathway.  It is
important  that EPA not lose  sight of other
potentially significant exposure pathways such
as emissions  from point sources- to-water-to-
fish and  possible  exposures  from cigarette
smoking.

       In terms of concentrations,  existing
data suggest  that fish have a higher  average
concentration than any other food, although
the ingestion rate is  lower. In terms of human
exposure, foodfish (especially freshwater fish
from the larger waterbodies) are likely to be
exposed  to  additional  point  sources  of
         After deposition on plants, particles are washed
into the soil by precipitation and plants die and decay into
soil, so that ultimately the deposited dioxins accumulate in
the soil; consequently, for grazing animals, this pathway
includes intake of dioxin via ingestion of both plants and soil.
PCDDs/Fs such as publicly operated treat-
ment works, as well as to air emissions.  For
this  human exposure scenario, such  sources
may be of equal or greater importance than
the air emissions.   Given that some popula-
tions eat larger quantities of fish (e.g., anglers,
Native Americans, etc.), the water-fish path-
way  deserves  additional  discussion as  an
   J
important exposure pathway.

        The weaknesses in the conclusion
that the  air-plant-food pathway is dominant
are carefully examined in Volume III, Chapter
7. One major conclusion about beef concen-
trations is that the "Total TEQ concentrations
compare favorably with observed total TEQ at
0.48  ppt and predicted TEQ  at 0.36 ppt."
One concern is using TEQs to support this
conclusion. This comparison, using the sum
of 17 values, each of which is multiplied by a
factor ranging from 1 to 0.0001, is misleading.
The  total TEQ concentrations between pre-
dicted and observed differ by a  factor of 4
(8.15 to 2.13) and ratios of observed to calcu-
lated values for individual congeners varies
from 0.13 to 22, roughly two orders-of-magni-
tude. Here, and throughout the document,
the  EPA must  move away from  the TEQ
comparison and begin to assess by individual
congener comparisons. When this is done, the
strength  of a number of EPA's  conclusions
about exposure and consequent human  risk
decreases.   Another way  to compare these
results is to note that 9 out of the  17 or about
53 percent of the congener values differ by a
factor of  5 or more.

        The same type of analyses are carried
out for the air-to-hay pathway calculations.
Unfortunately, there are only observed (other
than non-detect) values for five congeners to
make the comparison. For those five values,
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the observed concentrations are greater than
the modeled ones.   The analysis concludes
that, "Given the range of the detection limit,
0.31-6.4 ppt for the hay sampling, the model's
predictions of grass concentrations are gener-
ally consistent with observations,  with the
exception of the OCDD and OCDF concentra-
tions."   Two of the five  comparisons are
OCDD and OCDF, and the lack of data for
the other comparisons  cannot verify  the
model or conclusions.

       The fairly good agreement in TEQs, the
sum of the total concentrations, and the fact that
most of the individual congener comparisons agree
within  an order-  of-magnitude, all support the
conclusion that the air-plant-animal pathway is the
major input to the food chain. As noted above, this
is a worthwhile hypothesis and may well be  true;
but given  the lack of data and the number of as-
sumptions needed, it can not be proved at this time.
The document should state this fact explicitly and
might also note that no good alternatives are avail-
able.

3.2 A   Smoking—An Additional Potential
       Exposure Pathway7

       There is an additional exposure path-
way  that should  be considered.  Cigarette
smoking  may  account  for a very significant
fraction of dioxin exposures for some of the
population (25% of adults). Smoking has not
been  considered in the  reassessment docu-
ment;  although the  Committee does  not
consider  this  omission to be  a major flaw,
smoking should be considered in future  revi-
sions.  There are now three  papers  in the
              ,                 PAGE   25

literature that report that dioxin is present in
mainstream  cigarette  smoke.    Muto  &.
Takizawa (1989) estimated that a pack-a-day
smoker has a daily intake of about 4.3 pg of
polychlorinated  dibenzo-dioxins per  kg  of
body weight.  Lofroth and Zebuhr's (1992)

       The Agency should also
          consider possible
  contributions to exposure from
   environmental tobacco smoke
	(ETS).	

measurements of mainstream smoke imply an
intake of 18 pg TEQ per day per person for a
pack-a-day smoker.  This is  about 13% of the
daily median intake  estimated  from other
sources in  the  EPA exposure  assessment.
There  is also a paper by Ball et al. (1990)
which  reports  dioxin  emissions  for  main-
stream smoke that  are about an order-of-
magnitude lower than those reported in the
other two publications. Although differences
of an order-of-magnitude in dioxin measure-
ments  are common, there is reason to suspect
that they may have had substantial losses in
their sampling apparatus.  Beck et al. (1994)
found no differences in PCDD/PCDF content
of human breast milk between smokers and
non-smokers.  Nonetheless, given that  the
exposures are actually distributions, and that
some members of the population are heavy
smokers, this source  should be considered.
The Agency should  also  consider possible
contributions to exposure from environmental
tobacco smoke (ETS).  It would be useful to
know if any good data on the dioxin content
(or  lack of  dioxin  content)  in  sidestream
smoke exist.
         This discussion does not relate to a specific
question in the Charge. The issue of smoking as a possible
exposure pathway arose during the preparation of this report.
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PAGE  26
3.2.5  Anthropogenic vs. Natural
       Sources of Dioxin (Charge Ques-
       tion 10)

       The  background for  this question is
the observation in the late 1970s that dioxins
are produced  by the  combustion of many
common materials, including municipal solid
waste.  This led some scientists to suggest
that  dioxins had  been with  us since "the
advent  of  fire"  and that dioxins could be
produced by natural combustion (for exam-
ple, by forest fires). At that  time, there were
some suggestions  that observed  levels of
dioxins  were  primarily the result of  coal
combustion or perhaps of wood burned in
small stoves.  This  speculation was largely
refuted by sediment core studies, both in the
United  States  (primarily in the Great Lakes)
and in Europe, which indicated that environ-
mental  dioxin levels increased  significantly
beginning about 1935-40  (see Volume II,
pages 3-92  to 3-94).  Since the advent of fire
clearly predated this time, it can be concluded
that dioxins were largely anthropogenic and
associated with  events  taking place around
1935-40.  What were these  events?  Coal
combustion could  be ruled  out because the
consumption of coal in  the United States was
essentially  constant  from the turn of the
century until about 1970; this  record did not
agree with  the  sediment core  data.   The
explanation is likely to  be the introduction of
chlorinated  organic  compounds (polyvinyl
chloride  and chlorinated pesticides are but
two  examples) in  the  1935-40 time-frame.
Other sources  such as leaded gasoline (which
commonly contained ethylene  dichloride and
ethylene dibromide), diesel emissions,  and
PCBs are also possibly significant contribu-
tors.  Although the details of dioxin formation
are not  yet quantitatively  understood, the
introduction  of these  chlorinated  products
into wastes that were combusted appears to
be  the  most likely  cause  of the increased
dioxin deposition measured in sediments.

        These  sediment core data are  now
numerous.  Several  such studies have been
published for lakes from throughout the world,
and these studies have not been challenged in
the scientific literature. Therefore, it is very
clear  that dioxins  are  a Twentieth Century
phenomenon closely correlated with the pro-
duction  of  chlorinated  compounds.   The
Committee concurs strongly with the conclu-
sion that "environmental levels of dioxins are
primarily a product of human activities in the
twentieth century."  The draft document could
make this point even  more  persuasively by
citing all  of the sediment core  studies  that
have  been published,  even though some  of
them have been for lakes outside of the U.S.

       Incidentally, there is an undercurrent
of opinion (which  has also been expressed in
the public comments received about the draft
reassessment) which says that "forest fires are
possibly the  major source of dioxin  in the
environment." The Committee concludes that
this contention is  not  correct.  Many of the
sediment core studies in the  scientific litera-
ture span times during which forests in the
lake's  watershed  were burned by natural
causes.  There was no elevation of dioxins in
the sediment record at the time of these forest
fires. A recent study by Buckland et al. (1994)
found no difference in soil dioxin levels  in
Australian conservation areas before and after
brush fires.   Consequently, the  Committee
concludes that environmental  levels of dioxins
are primarily a product of human activities in
the Twentieth Century.
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                                                                                 PAGE  27
             There is  one other feature of these
      sediment core data that warrants comment.
      Most of the sediment records show a decrease
      in dioxin deposition starting around 1970; in
      other words, dioxin deposition from the atmo-
      sphere to lake sediments was at  its  highest
      around 1970.   This  decrease seems to be
      continuing, and it may well be attributable to
      decreased emissions from large-scale combus-
      tion systems; regulations for such systems are
      not thought to have had major impacts prior
      to the early 1980s; consequently, the origin of
      the decline is still unclear.

             Beyond the sediment core studies, the
      history of dioxin emissions has been addressed
      by two studies (Ligon et al, 1989; Schecter,
      1988)   of  mummified  human  tissue  (see
      Volume II,  pages 3-149  to 3-150).   These
      results suggest that dioxins were present only
      at very low levels in humans at the time these
      individuals were mummified  (about 2800
      years ago in  the Ligon study, and 400 years
      ago in the Schecter report).  The presence of
      these compounds in modern humans at much
      higher concentrations is well known; there-
      fore, these ancient tissue analyses support the
      concept that dioxins have not been with us
      since "the advent of fire," but are  a more
      recent addition  to our environment.   How-
      ever, unlike sediment core studies, these tissue
      measurements, in addition to being limited to
      only two samples, do not tell us when dioxins
      became an important part of the environment.

      3.3    Uncertainty in Estimating Human
             Body Burdens  (Charge Question
             11)

             Body burden  data  are presented in
      Chapter 5, pages  5-18 to 5-27 of the exposure
      reassessment document. These data  provide
one index of exposure to dioxins; the data on
body burden  are also used to estimate expo-
sures using a pharmacokinetic  model that
back calculates the dose needed to achieve the
observed  adipose  tissue  levels  (assuming
steady state  exposure/dose).   The principal
U.S. data come from the National Human
Adipose Tissue Survey (NHATS). Table 5-8
presented mean adipose tissue data for a
number of congeners for 865 samples  col-
lected in 1987 and analyzed as 48 composites,
each containing an average  of 18 specimens.
Analyses are  reported as showing increasing
levels with increasing age and as being lower,
in 1987 in comparison with previous findings
from 1982.  Except  for one  congener there
was  no variation by  region  and no variation
for any congener by race and sex. Additional
data  are  provided  from U.S.  reports  by
Patterson  (1994) and Schecter (1991). Data
from Germany and elsewhere  are also cited.
The  report assumes a level of 2,3,7,8 -TCDD
in adipose tissue of  5.0 to 6.7 ppt  for the
purpose of estimating typical exposure.

       The Committee believes that reliable
nationally representative body burden data
are not available. The reassessment acknowl-
edges that the NHATS data, the most exten-
sive available, cannot be considered as repre-
sentative,  and the smaller data sets are even
less likely to  estimate average exposure accu-
rately. Because the exposure  histories of the
individuals included  in the NHATS studies
are unknown, the average  could be biased
upwards by occupational exposures or residen-
tial exposures from smoking.  However, there
would be little impact on  the mean unless
exposure markedly increased levels, given the
sample size of 885 samples  in NHATS. The
Committee  anticipates that  only  a small
proportion of the population would be heavily
./OX'.' REASSESSING DIOXIN

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PAGE  28
contaminated.   On  the other  hand, the
possibility  remains  that selection  of the
samples  may  have been weighted towards
more exposed  persons.   The  reassessment
document does not attempt to estimate the
potential for such bias nor its  consequences.

       Although the  NHATS  data may be
sufficiently robust to  provide  a  reasonable
estimate of the mean, the  range of tissue
concentrations is not provided  by these data
because the samples were pooled for analy-
sis.  Further, because of the modest sample
size, the data  are not adequate for  trends
analysis  and would  have limited statistical
power  for  any comparisons  across  groups,
whether defined by race, sex,  or geography.
Thus, the findings of the analysis on these
factors should be given little weight.

3.4    Estimating Background Expo-
       sures  via Food/Body Burden
       Data (Charge Question 12)

        The  Committee agrees  that it  is
appropriate  to  use   food data  and food
factor information to  estimate exposure to
the 2,3,7,8-TCDD.  It is also appropriate to
use body burden data on TCDD to estimate
daily uptake from all  sources  (half-life of
about 7.5 yr).

      As noted  earlier,  the  food analysis
data  are too  limited  to extrapolate the
results  to  reflect  the  distribution for the
entire U.S.  population.  Data obtained from
different geographical areas, with a statistical
emphasis on those foods that contain the
bulk of the dioxins and furans, are needed
before  these  data  can be used to  estimate
uptake.   The  Committee also recommends
that EPA evaluate cigarette  smoking as a
possible exposure pathway for dioxins  (see
discussion in section 3.2.4).

3.5    Site-Specific Assessment Proce-
       dures

3.5.1  Steady-State Assumptions and
       Modeling  (Charge Question 13)

       The Committee  did not  raise objec-
tions to or have comments on  the  validity
of the steady-state  approach or  challenge
the model selection.  It was noted, however,
that in future efforts it would be desirable
to have some estimate of the importance of
long-term  accumulation in sinks such as
sediments  or soil.

       There were also some concerns about
the potential input of harbor dredging  and
storm  events on re-suspended solids.  Al-
though  these  occurrences  probably  have
minor impacts on the exposure of the total
population,  they might impact on  certain
sub-populations with high fish intakes.  This
comment is  intended to point out the need
for future work  and to describe  potential
risks  that  may not be addressed  in  the
present system of analysis.

3.5.2  Mass-Balance Issues   (Charge
       Question  14)

       On page 3-55 to 3-57 of the expo-
sure reassessment document,  where  the use
of the COMPDEP  model  is described, it is
stated that  the  model  should be  run with
no  deposition for gas-phase  contaminants,
and  with  both  wet  and  dry  deposition
included for particles.  Given the nature of
atmospheric dispersion models, this  implies
that, for  gas-phase  CDD/Fs,  the   plume
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                                                                             PAGE  29
 disperses with full reflection at the ground
 surface, and for particles there is a loss at the
 ground surface based on the flux attributable
 to the wet and dry deposition.  This reflects
 the state-of-the art in atmospheric dispersion
 modeling.  Nevertheless,  this  approach ig-
 nores  some significant mass   balance  and
 energy balance  issues with regard to the
 dispersed CDD/F species.   To understand
 these  issues, the gas-phase  CDD/Fs at the
 interface between air and the soil, at vegeta-
 tive surfaces, or  at surface water with which
 they come  in  contact, must be considered.
 This is especially true, since EPA believes that
 this is the  predominant mechanism for the
 contamination of food. Even though  these
 gas-phase contaminants are  not expected to
 "deposit" physically  onto these surfaces, a
 partitioning in response to the tendency of
 natural systems to maintain thermodynamic
 equilibrium is expected.  Consider that in the
 atmosphere both the  particles and  pure gas
 phase   provide  comparable  reservoirs  for
 CDD/F   congeners.      Since   the   sur-
 face-to-air-volume ratio of the ground surface
 and vegetation surfaces are much larger rela-
 tive  to  the  atmosphere  than  the  sur-
 face-to-volume ratio of air particles, how can
 the  partitioning of  CDD/F compounds at
 air/plant and air/soil interfaces be ignored? In
 an air/soil, air/plant,  or  air/water  system,
 chemical thermodynamics  is likely to favor a
 relatively large fraction of the chemical in the
 non-air compartment.  Consequently, there is
 a  fairly strong chemical potential  likely to
 drive the chemicals from air into these other
 media, where they are likely to be  retained,
 transformed, and re-emitted, either in the gas
 phase or bound to particles.

       These  types of processes cannot be
 captured using  the  COMPDEP (Complex
Deposition) model but instead require chem-
ical  potential  models.  At this time, we
cannot ascertain the magnitude of any errors
thus  introduced  to  the   output  of  the
COMPDEP model,  or their significance to
the  assessment.   In  order  to  explore the
significance of the omissions of mass-balance
in the COMPDEP  model, there is a  need
also  to  develop   a  regional-scale  chemical
potential model and apply it individually to
the CDD/F compounds. It is important hereto
emphasize  that these  types  of transport  and
transformation  processes  are  compound-   and
congener-  specific and  therefore should  not be
estimated  on a  TEQ basis.   Until such exten-
sions can be developed and applied, the use
of COMPDEP remains the best alternative.

3.5.3  Model Validation  Issues  (Charge
       Question 15)

       The Committee considered the  com-
parisons of model-estimated versus measured
environmental contaminant concentrations.
It was noted  that the narrative  material in
the  reassessment document described seven
such comparisons, and no generalizations
about model function could be easily drawn.
It appears that there has been no overstate-
ment of  model validity and that a consider-
able effort has been  made to uncover all
useful  methods  for validating  the models
with the existing data.   It was noted by
EPA that the validation exercise for the beef
bioconcentration model algorithm was  per-
formed and that the  results  supported the
model.    There   were no suggestions for
alternative model use, but the review of the
earlier SAB  guidance on  peer review  and
model validation (SAB,   1989)  should  be
discussed in the document.
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PAGE  30
       The  Committee  also  discussed  the
distinction between model validity and model
system validity.  The existing system of analy-
sis  will not answer questions  of  long-term
contaminant accumulation in sinks and would
be  difficult  to  adapt for use  with multiple
sources. The Committee suggests that if these
other important questions are to be addressed,
a different framework will have to be devel-
oped.

       It is important to note that the above
comments are  not intended to suggest that
the  current framework is  inappropriate,
inaccurate,  or  in need  of  revision for its
stated  use  as  a  data   and   methodology
resource for risk assessment.

3.5.4  Photolysis and Atmospheric
       Transport  (Charge Question 16)

       The  relative abundance of the vari-
ous dioxin homologues and congeners differs
between sources  and the environment and
environmental  sinks.   For example,  most
combustion  sources generate a dioxin mix-
ture with relatively high concentrations  of
tetrachloro- and pentachloro- dibenzofurans,
whereas the environmental  sinks are domi-
nated  by relatively high concentrations  of
octachlorodioxin.   It  has  been  suggested
that the lesser chlorinated  compounds  are
degraded between the source  and the  sink
and that  the  mechanism for  this  degrada-
tion is  photolysis (see Volume II,  pages  2-
30  to  2-35 of  the  exposure  document).
The significance  of photolysis  is a difficult
question for the Committee  (or the EPA)  to
answer  because there are virtually no data
on  this subject.   Photolysis  is   probably
important, but  all evidence  on this point is
qualitative and indirect.
       During the atmospheric transport of
dioxins, they can exist in one of  two forms:
either  in the  vapor-phase  (in  which  the
compounds  are airborne as individual mole-
cules) or in  the particle-phase (in which the
dioxins are  sorbed onto atmospheric parti-
cles).  Photolysis of dioxins in each of these
two phases  proceeds at different rates and
by  different mechanisms.   Unfortunately,
there are very few data on  either of these
mechanisms.   Data  on  the photolysis  of
dioxins  from the  atmospheric particle-phase
are limited  to one  study, which indicates
that dioxins  associated  with  fly-ash   are
stable  when  exposed  to  simulated atmo-
spheric photolysis conditions.    Of course,
this is  only one  study which  needs to be
replicated.

       Two  mechanisms  for the degradation
of dioxins in the vapor  phase  are  possible.
The first is  direct photolysis, in  which  the
molecule of interest reacts with  a photon.
The very limited extant data indicate  that
2,3,7,8-TCDD has a half-life of a few min-
utes under  these conditions (Orth  et  al,
1989).   The  second mechanism, which is
probably the most important, is the reaction
of  dioxins  with  hydroxyl (OH)  radicals.
Most atmospheric chemists consider this to
be the  primary mechanism  by which organic
compounds  are removed  from  the  atmo-
sphere.   Thus,  determining the rate  con-
stants for reactions of dioxins with OH has
been  of considerable  interest.   Unfortu-
nately,  experimental difficulties  have  pre-
cluded  the  direct  measurement of these
values.   In the  absence of  experimental
data, models  (based on  various  substituant
effects) have  been  used  (Atkinson,  1987;
Atkinson, 1991).  These  calculations indi-
cate  that   the  atmospheric  lifetimes  of
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                                                                           PAGE   31
dioxins and furans are in the range of 1  to
40 days.   The tetrachloro- congeners are  at
the  low  end  of  that  range,  and the
octachloro- congeners are  at the high end.
Reactions  with  OH are considerably slower
than  those  induced  by direct  photolysis.
(Incidentally, this work on OH reactions
was not presented in the draft reassessment
document, and  it should be added.)

      There are clearly insufficient data  on
which to base any firm conclusions about the
general issue of atmospheric photolysis.  It
seems likely that important loss processes in
the atmosphere are photolytic and that reac-
tions  with OH are important.  However, rate
constants  for  none  of  these  reactions  are
known,  and it  is impossible to assess their
significance.

      Based on the modeled rate constants
cited above, it is likely that the lower chlori-
nated compounds are degraded more rapidly
than the higher chlorinated compounds. For
example, the tetrachloro- dioxins are probably
degraded more  rapidly than the  octachloro-
dioxins.  This estimate agrees  with observa-
tions  that the  less  chlorinated dioxins and
furans are reduced in relative  concentration
between  environmental sources  and  sinks.
However, it should be emphasized that this is
all mere speculation in the  absence of any
data on the relative rates of  the pertinent
reactions.

      Photolysis may also be important in
other parts  of  the  environmental transport
scenario for dioxins.  Photolysis in water must
be considered after  deposition  of these com-
pounds  from  the atmosphere to lakes  or
oceans.  Photolysis on soil and on plant sur-
faces must be considered as these compounds
begin their movement into the human food
supply.  In all of these  cases,  data on pho-
tolytic reactions are very sparse.  In addi-
tion, a knowledge of the  products of these
environmental transformations  is not avail-
able.  Is it possible that congeners with low
TEFs are being transformed in the environ-
ment  to  congeners  with  higher  TEFs?
Clearly these  are  all areas  in which addi-
tional research is needed.

3.5.5   Air-to-Plant Transfer (Charge
        Question 17)

 As stated in the Charge, the question of
the importance of air-to-plant transfer is too
difficult to  answer at  this  time with the
available  data.   The  air-to-plant  transfer
coefficients suggested by EPA are reasonable
for 2,3,7,8-TCDD (given the available data),
but there are insufficient data to support the
assumptions for the transfer coefficients for
the other PCDDs and PCDFs.

   It is widely thought that some
   fraction ofPCDD taken up by
 grazing animals will normally be
    associated with ingestion of
          contaminated soil.	

       For EPA to understand the  impor-
tance of the issue of air-to-plant transfer, the
fundamental physical and chemical proper-
ties for most PCDDs and PCDFs need to be
determined (and confirmed).  Values for K^
(the octanol/water partition  coefficient) and
vapor pressure, water solubility, and photol-
ytic half-life should be  near the top of the
list of data gaps that  need to be filled to
answer this question.
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PAGE  32
       It  is not possible  to work backward
from  concentrations of CDD/Fs  in  cows to
estimate what was on the plant which the
animals ingested, because of  the significant
contribution of soil ingestion by grazing cows
to the total uptake.  It is widely thought that
some  fraction  of PCDD taken up by grazing
animals  will  normally  be  associated  with
ingestion of contaminated soil.  Ideally, a high
quality field study of air concentrations of
CDD/Fs and CDD/Fs versus the concentra-
tion in vegetation would be the basis for the
air-to-plant transfer coefficient estimate.  This
type of study would probably be performed in
several agricultural  areas.  Some  Committee
Members preferred this approach over labora-
tory studies because of the inability  to repli-
cate what happens in the outdoor  environ-
ment.

3.5.6  Vapor/Particle Partitioning
       (Charge Question 18)  .

       The  EPA document  presents  three
alternative approaches for the estimation of
vapor/particle  partitioning and discusses the
limitations of each. It  is important to note
that actual measurements of  vapor/particle
partitioning in air  are  technically  difficult.
Lacking such  data,  the model proposed by
Bidleman  (1988) was selected by  EPA for
estimating partitioning  as the best of the
three  approaches.

       One approach  considered  was  to
derive partition estimates from  stack  sam-
pling  data.  The inherent sampling  artifacts
(e.g., the use of heated filters to collect parti-
cles) introduced by current stack monitoring
methods were  recognized  as the main limita-
tion of this approach. A further limitation  is
the need  for  modeling the  changes in va-
por/particulate partitioning downwind  from
the source, as the plume components undergo
dilution,  condensation,  and   coagulation.
Given the effects  of these processes on va-
por/particle   partitioning  of  CDD/Fs,  the
model estimates would be quite unreliable
and must be considered  to be  preliminary
estimates.

       Another approach  consists of using
current ambient monitoring data.  The avail-
able data, however,  are limited with respect
to the particle size distribution and environ-
mental condition represented.  Furthermore,
these data have  been typically obtained with
high-volume   samplers  such  as the  PS-1;
particles are collected with a filter while the
vapor-phase compounds not retained in the
filter are captured by a sorbent trap located
downstream from the filter.  The main limita-
tion of these  data is  that part of the particle-
bound CDD/Fs collected by the filter may re-
volatilize, particularly when large volumes of
air are moved  through the filter.   Conse-
quently, the fraction  of the  CDD/Fs collected
by the sorbent  trap may  overestimate the
vapor phase  fraction actually  found in the
atmosphere.   Nonetheless, these data could
provide  an upper bound  estimate  of va-
por/particle partition.

       The  third approach,  and  the  one
selected  (as   noted   above)  for estimating
vapor/particle partitioning  (a choice which
the Committee  considers acceptable), is the
model proposed by Bidleman (1988), which
is  largely based on fundamental properties of
the CCD/Fs.   Phase partitioning is assumed
to  be conserved  between  the source  and
downwind receptor.   There are some limita-
tions to this  approach, such as the use of
solid phase vapor pressures at 25° C, whereas
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                                                                            PAGE  33
ambient temperatures  could  vary signifi-
cantly (the EPA document applies the model
for 20°  C).  This could be the major reason
why the calculated data in table  3-5, page
3-42  agree  so poorly  with the  measured
data.  However, the model results  appear to
be  in  general  agreement  with   the   va-
por/particle  partition  trend in the ambient
data,  that  is,  the  tetra- and  penta- substi-
tuted congeners  tend  to  partition to  the
vapor  phase,  and hexa/hepta  chlorinated
compounds are mainly particle-bound. Since
vapor/particle partition  depends on ambient
temperature, further evaluation of the results
from  the  model  could be  performed  by
comparing ambient data obtained during  the
temperature extremes of winter and summer.
The   Committee  recommends  that  EPA
undertake such an effort.

3.5.7  Background Exposures and Site-
       Specific Evaluation  (Charge
       Question 19)

       The  definition  of  "background"  as
applied in the current assessment is given on
page  5-1  of Volume  II as: "...background
exposures estimated in this chapter are based
on  monitoring  data  obtained from  sites
removed from  known contaminant sources
(or food data representative of the general
food supply.)"  On the other hand, the site
specific  assessment is directed at estimating
incremental exposure resulting from a spe-
cific source.  There seems  to be an implicit
contradiction in these two statements, since
the implications of an increment in exposure
from  the emissions of a given source are a
function of existing  conditions  (i.e.,   the
presence or absence  of  other similar  or
different sources).    Depending  on those
specific sources,  the  "background" default
assumptions of the six scenarios presented in
Chapter 5 of Volume III may not be appro-
priate. Certainly the data on environmental
concentrations  of   CDD/Fs  presented  in
Volume II demonstrate that there is signifi-
cant variability  in  levels  even among  sites
which could  be categorized in  the same
scenario.

       The term "background"  is also  con-
fusing because,  in  spite  of the preferred
definition stated above, it is used in  the rest
of the document to represent  other concepts.
For the purpose of site-specific assessments,
the term "baseline conditions" appears to be
more appropriate than "background."  The
Agency might then consider  providing guid-
ance for performing baseline exposure assess-
ments for specific sites. Such guidance  may
include the following  recommendations for
the user:

 a)     use site-specific media concentra-
       tions rather than defaults whenever
       possible;
 b)     if these data are unavailable, use
       inforrnation from a comparable site
       in terms of the types and density
       of sources present, land-use condi-
       tions, and g  ^graphic/atmospheric
       characteristics;
 c)     when site-specific or comparable
       site data are not available, use re-
       gional  data.

       The Agency might consider including
examples  of  how   a  site-specific baseline
evaluation  might be performed.   Consider-
ation  of  site-specific  baseline  conditions
would obviously implicitly include the  con-
tributions from existing multiple sources.
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3.5.8  Evaluation  of  Multiple
       (Charge Question 20)
Sources
       For the reasons discussed in detail in
section 3.5.7 above, it may not be appropriate
to utilize  the national background  exposure
estimates  presented in Volume II of the draft
report as  representing the site-specific back-
ground levels.

       Where a  site is impacted by multiple
existing sources of dioxin-like compounds, it is
appropriate to model the contribution of each
individual existing source and then sum their
emissions  and depositions for the purpose of
calculating a  baseline quantification of total
exposure  for  that  site.  Whenever  possible,
baseline calculations should be developed on a
media-specific basis. This baseline  exposure
calculation may  differ significantly from the
national   background  levels  discussed  in
Volume II. The dioxin ambient ait/deposition
map currently in development by EPA will
hopefully  provide information on  regional
deposition levels that can provide more local-
ized background level information.


   The EPA has done a good job
    of evaluating the sources of
    dioxins, based on available
                data...
       The  SAB Indoor  Air Quality/Total
Human Exposure Committee has previously
raised the concern in the context of its review
of the EPA draft document "Addendum to the
Methodology for Assessing Health Risks Asso-
ciated with Indirect  Exposure to Combustor
Emissions" that a more regional approach be
adopted for evaluation of exposure and health
risks from indirect exposures  to  combustor
emissions (SAB,  1994).  Dioxin-like  com-
pounds were among the pollutants of concern
with respect to these sources.  The Committee
recommends that guidance be provided in Volume III
to indicate that a regional, as well as site-specific,
exposure assessment be undertaken in  areas  with
multiple existing sources of dioxin-like compounds.

       Currently,  the draft  report provides
little information for the reader regarding how
to assess  baseline exposures at a site with
multiple  existing sources of  dioxin-like com-
pounds. The inclusion of a case-study example
would make the report more informative.

3.6  Overall Scientific Foundations of
     the Reassessment Document
     (ChargeQuestion 1)

     The EPA staff has done a very credible
and thorough job on a large and complex task.
They are to be commended on the work that
they have done to assemble, integrate,  and
analyze a very large body of data on source
emissions,  environmental levels,  exposures,
and human body burdens in the framework of
human exposure  assessment.  In  so doing,
they have uncovered key data gaps and issues,
developed  some   reasonable  priorities for
future  efforts, and  begun to implement re-
search  efforts to address information gaps. In
general, the work has been clearly presented
and the documents are well written. It should
also be noted that, as a result of this inte-
grated  assessment, a number of industries are
currently seeking to address gaps in emissions
measurement data.

     The EPA has done a good job of evalu-
ating the sources  of dioxins, based on avail-
able data,  and significant sources of uncer-
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tainty have been qualitatively identified.  In
addition, this document provides the founda-
tions   for  a  quantitative   treatment  of
uncertainties--a task that is, however, beyond
the scope of the current reassessment docu-
ment. Although there is great uncertainty in
the levels of dioxins in some environmental
samples, these levels are consistent with the
emission inventory; that is, comparison of the
emission inventory and environmental levels
does  not imply that there are  significant
unknown sources.  There is also some evi-
dence,  i.e., decreases  in concentrations  of
dioxins in surface sediments and human tissue
samples,  that  indicates that  emissions  of
dioxin are decreasing. Further environmental
and human monitoring is, however, required
to confirm this.

       In  assessing sources  of   dioxin-like
compounds, the total estimated dioxin emis-
sions  for the U.S.  have been compared to an
estimate of the total amount of dioxin that is
deposited to the surface of the U.S. based on
available deposition factors.  The difference
between the two  estimates  has raised some
concerns  about possible  "missing sources."
There is  a serious  scientific problem with
trying to perform such  a  simplified  mass
balance  on a continental or regional scale.
That is, the atmospheric lifetime of accumula-
tion mode particles (0.1 to about 2-3 pirn) is of
the order of many days. Thus, a large propor-
tion  of the  particles with  dioxin-like  com-
pounds that are emitted in the eastern U.S.,
for example, are likely to be deposited in the
Atlantic Ocean, Canada, Europe,  or even the
Arctic, depending upon source locations and
weather patterns.  Given the long atmospheric
half-life  of  the  participate  dioxin-like  com-
pounds, a global or hemispheric mass balance
is  necessary.  A second, and  perhaps even
 more serious concern, is that the mass balance
 calculations have been performed using only
 two data points for deposition in the  U.S.
 (Bloomington, Indiana and Green Lake,  New
 York).   These locations  are  unlikely to be
 representative  of average  deposition in this
 country.   Given  these problems, it  is strongly
 recommended that the sections of the report in which
 the very simplified mass balance comparison of the
 emissions inventory and the  deposition mass are
 compared be modified so that a direct comparison is
 not made and it is made clear that such a compari-
 son is not scientifically valid.  In addition,  these
findings also mean that the simple mass balance
 presented here  cannot  be used to infer "missing
 sources."

      The  available  scientific   evidence
 strongly indicates that current levels of di-
 oxin-like compounds in the environment are
 largely derived from anthropogenic  sources
 (see section 3,2.5).  There is also considerable
 scientific evidence that the principal mecha-
 nism by which dioxin-like compounds enter
 the terrestrial food chain is via atmospheric
 transport and deposition.  However, there is a
 very  large gap in our understanding  of the
 potential  atmospheric  transformation of
 vapor-phase dioxin-like compounds;  specifi-
 cally, there are no experimental measurements
 of photodegradation or degradation via  reac-
 tion with hydroxyl  radicals.  Environmental
 measurements of deposition of paniculate and
 vapor-phase  dioxin-like  compounds  to  the
 surface are also extremely limited, although
 we understand that there are now some efforts
 to address this data gap.

       The evidence that the principal path-
 way for human exposure  to dioxin- like com-
 pounds is through the diet, with consumption
 of animal  products  accounting  for   the
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PAGE  36
dominant  or   overwhelming   fraction  of
exposure,  is  also quite robust.  The  associ-
ated  air/plant/animal  pathway  hypothesis
has  considerable support, although  it  has
not been  proven unequivocally, particularly
in view of the very limited  available data.
It is  also important  not to lose sight of
other potentially significant dietary exposure
pathways,  such  as emissions  from  point
sources to water or sediment, and then to
food.   In addition,  cigarette smoking  should
be  evaluated  as  a  potentially significant
exposure source for smokers in  the popula-
tion (about  25% of U.S. adults are  smok-
       If the estimates of dioxin-like com-
ers
pounds in beef and pork presented  in  the
report,  based  on  very  limited  data,  are
found  to  be  too  high, then cigarette smok-
ing (for which the dioxin estimated are also
based  on very limited  data) could account
for a significant  proportion of exposure for
smokers.

         Estimates  of  human  exposures,
based  on currently  available  information
from   exposure   analysis  and   analyses  of
human tissue samples,  are  consistent and
are likely to provide  a  reasonable  central
estimate of the distribution of exposures for
the population.   Existing measurements of
dioxin-like compounds in food,  however, are
very limited.   Consequently, we  lack  data
that would allow  an estimate to be made of
the distribution  of exposures for the  U.S.
population.

       The   Committee  found  the  term
"background"  somewhat  confusing  and
suggests  that"  the national average  back-
ground   exposure,"    or   some    similar
explicit term should be  used in future drafts
for greater clarity.

      The Committee also believes that the
very brief discussion  and  recommendations
on multiple sources should be substantially
expanded and that more  detail should  be
provided.  Of the two approaches suggested,
it was felt that the better approach would be
to model multiple  sources in a  region and
then  make   comparisons  of  the potential
exposure  contributions  of  a  new source  to
local exposures. The results might be termed
"baseline," and  considered  along with  na-
tional average background  exposure, as well
as with increments in exposure  from a spe-
cific  facility.

      Understandably,  the  focus   of   the
report is on site-specific models, which are
needed for  regulatory  purposes,  e.g.,  to
answer questions  such  as  "Is  a proposed
facility likely to result in a  significant in-
crease  in  exposure  above   background?"
Ongoing work to  refine,  test, and validate
these models can  be  considered a  "micro-
scopic" approach.  It would, however, also  be
very useful to have a  macroscopic approach,
including  more regional  and global  mass
balances and multimedia modeling.  That is,
other types   of  models should  be  used  to
examine other issues.  For example, the very
similar  levels of  these compounds  which
have been found in food and humans  in a
number  of   countries,  suggests  that  they
probably  are  widely  distributed via atmo-
spheric transport processes throughout  the
Northern Hemisphere and that  more global
(or hemispheric) evaluations of mass  balance
should  be  undertaken.
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                                                                            PAGE  37
               4. DETAILED FINDINGS-HEALTH DOCUMENT
4.1    Disposition and Pharmacokinetics
       Issues

4.1.1  Strength of the database  (Charge
       Question 2)

       The Charge for this review (see section
2.2) states that "An understanding of the
relationship between exposure and dose is an
important aspect of an adequate characteriza-
tion of risk."  A specific concern  is how the
extant data are used to predict tissue dose
levels of 2,3,7,8-TCDD in humans under low
exposure conditions.

       There is a  an extensive animal data
base  relating  exposure   to  tissue dose  for
2,3,7,8-TCDD  (although data are lacking for
many of the related compounds), and there is
a substantial (and generally solid) body of
data on  the  absorption  and distribution of
2,3,7,8-TCDD  in animals (Bimbaum, 1985;
Gasiewicz <*«/.,  1983; Neal etai, 1982; Olson
etal, 1983; Van den Berg etal, 1994). There
are insufficient human data to support deposi-
tion and tissue dose modeling however, and
this data gap severely restricts animal (largely
rodent) to human extrapolation.   Further,
there are only a limited number of animal
studies that reflect accurately likely environ-
mental exposure scenarios for humans.  For
example,   gastrointestinal   absorption  of
2,3,7,8-TCDD  is influenced by the presence
of other compounds, the nature of the matrix,
and the  very limited aqueous solubility of
2,3,7,8-TCDD.    These and  other factors
relevant to the  partitioning of 2,3,7,8-TCDD
and gut  absorption  are  of  critical  concern,
given the fact that foodstuffs are considered
by EPA to represent a major source of chronic
low level human exposure to dioxins .

     The pulmonary exposure data base is
based  on the  pulmonary  absorption  of
2,3,7,8-TCDD from solution;  absorption by
this  route is  known to be high.  The most
likely non-dietary human exposure to 2,3,7,8-
TCDD, however, would be through inhalation
of paniculate matter incorporating  dioxin in
a solid matrix (e.g., fly ash). Although data on
pulmonary absorption from a solid matrix are
available  (Nessel  et al, 1990; Nessel et al,
1992a; Nessel et al., 1992b), these data are
not addressed in the reassessment document.

     Given the large data base, a more thorough
analysis of the biological determinants of tissue
absorption and deposition (particularly with low
dose exposure) should be carried out.  For example,
mice show a different pattern of distribution
to the  skin than rats, with the former more
accurately reflecting data obtained in non-
human primates  (Brewster and  Birnbaum,
1988; Gallon/., 1992; Rahman etal., 1992).

     The hepatic  distribution  patterns  of
2,3,7,8-TCDD in animals appears to be dose-
dependent and saturable. This is an area that
needs further study, particularly with regard
to potential interspecies differences and the
development of valid human physiologically-
based pharmacokinetic (PBPK) models.

     In  general, the reassessment document has
drawn  extensively from the existing animal data
relating   exposure/tissue   dose   and  absorp-
tion/distribution,  but did not characterize ade-
quately the strengths and weaknesses  of the data-
base.  Further, the  inferences drawn leave some
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PAGE  38
issues unaddressed. A greater effort needs to be
made to describe the long-term effects of the
decrease of PCDD- and PCDF levels in the
environment, and  in turn, in human  expo-
sures.   This description  should  provide an
improved estimate of the mix of chemicals in
the  TEQ and  their  capacity  to be  both
agonists and antagonists (or synergists) in the
overall biological effects of 2,s',7,8-TCDD and
2,3,7,8-TCDD-like compounds,  as  well as
those  chlorinated  compounds that  are  re-
viewed in the health assessment documents
but  are not 2,3,7,8-TCDD-like. In addition,
to enhance  further the  value of the reassess-
ment,  a greater  effort is needed to provide a
better understanding of the consequences and
possible interaction of employing both the
receptor model  for risk assessment and the
utilization of the concept of TEQ. For exam-
ple, what can be expected in this context with
a range  of halogenated chemicals  that may
interact in various ways as either agonists or
antagonists? What can be expected from a
mixture of such compounds, when considering
both additive or non-additive effects, with
constant or changing concentrations of those
individual components  which contribute to
both response and the TEQ value?

       In chapters I through 7, which mainly
review the current research  data, the  docu-
ment provides  a generally balanced  evalua-
tion, and inferences from these chapters are
appropriate  except as noted above.  However,
Chapter 8 presents an  incomplete review of
the dose response model (Linear  Multi-Stage
[LMS] and modified LMS) approach,  with no
consideration of alternative  models used by
other agencies, nor discussion of the literature.
This is  reflected  in the  summary chapter.
Thus, the Committee recommends that EPA provide
either additional   discussion  of alternative ap-
proaches and their implications for risk assessment
in Chapter 8, or present a  clear justification for
choosing this particular dose-response approach over
others.  Chapter 9 should also be modified to reflect
these additions.

4.1.2   Disposition and Pharmacokinet-
        ics (Charge Question 3)

      In addition to the  specific questions
asked about disposition and pharmacokinetics
by  the Charge, the introductory sentence
leading into this issue must also be evaluated.
This  sentence   states  " The evaluation of
available  data  and  the development  of
physiologically-based models  has led  to a
better understanding of the  disposition and
pharmacokinetics of dioxin and related com-
pounds than for most other environmental
chemicals." This sentence implies that these
models are well-established and ready  for
incorporation into the current assessment. It
is the Committee's consensus, however, that there
are  several issues  that should be addressed before
these  models can  be used effectively  in the risk
assessment.  The following questions should be
addressed in a revised assessment document:

  a)   Does the database to determine mecha-
      nism and models apply only to 2,3,7,8-
      TCDD or may it be extended  to  other
      related compounds?   Currently the
      great majority of data on physiologically
      based kinetic  models is  derived from
      2,3,7,8-TCDD research, but about 90%
      of estimated "risk" is from related com-
      pounds (as stated in the reassessment
      document, p. 9-81).

  b)   Do  the models proposed extend  to all
      dose ranges, particularly to low  dose?
      The  data  used in current models were
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                                                                            PAGE  39
not generally derived from studies conducted
at low dose levels. There is significant contro-
versy as to the shape of the curve in the mod-
els proposed at the low dose levels.

 c)    Are all organs effectively addressed in
       the models?  The models appear to
       focus  on liver and fat concentrations
       in rodents; however, the target organs
       in  the epidemiology studies for carci-
       nogenesis are the gastrointestinal tract
       and others.  It is not clear  that  the
       models effectively describe the expo-
       sure and metabolism of those possibly
       impacted tissues. Particular questions
       may exist with respect to the lung for
       carcinogen exposures that occur by the
       respiratory route (although this route
       is probably of minor significance for
       most of the population, it could be of
       concern for occupational exposures).

 d)    If models have  primarily been deter-
       mined from short-term or single-dose
       studies,  do  these results  apply  to
       chronic  studies  and/or  longer term
       exposures?  In particularf;the chronic
       Kociba et al. (1978,  1979)  studies,
       which  included low  exposure levels,
       did not necessarily fit one of the mod-
       els proposed, whereas the dose finding
       studies did.  A question then exists
       concerning the model's efficacy for the
       real life-low exposure situation.

 e)    Do the models apply across relevant
       mammalian species?

 f)     What  is  the  relationship  between
       "dose" as used in the dose-response
       models and tissue or body burden that
       the EPA uses in many comparisons
      between human and animal exposures
      and risks?

      Lastly, the variability of the half-life of
2,3,7,8-TCDD in the human leads to ques-
tions about trie precision of the models. The
estimated half-life discussed varied from five
to 11.3 years.   The range  given  here may
represent a difficult communication issue, and
affects the precision of the model.

      In the body of the health reassessment
document, the  issue of PBPK  modeling is
discussed  in  three  chapters (1,8,  and  9).
However,  there  is  little evidence of actual
integration of any of these models into spe-
cific portions of the document.  The concept
of physiologic-based modeling  is discussed
separately in the risk assessment portions of
the document, and  is conceptually a part of
the process. Scrutiny of the document (and
oral discussions with the document's authors
at the public meeting)  however, yielded no
evidence of use of a specific P%PK model in the
risk  assessment.    Given the  issues noted
above, this is understandable and not consid-
ered to be a particular Weakness by the Com-
mittee (however, opportunities do exist—see
the discussion below), but future revisions of
the document should be clear as to the degree
to which such, modeling has been incorporated
into the assessment  process.

      Notwithstanding the lack of incorpora-
tion of these models into the current reassess-
ment and the  significant  questions  noted
above, the database on dioxin could provide
an opportunity to utilize state-of-the-art PBPK
models.  The  Carrier-Brunet-Brodeur model
(Carrier et a/.,1995a; 1995b) (which was not
yet published at the  time the Health Reassess-
ment was under development), for example,
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incorporates non-linear elimination rates; as
body burden declines, half-life increases. The
applicability of this model and its implications
should be discussed in any future revision of
the reassessment document. The opportunity to
utilize the Ah receptor binding  in modeling is
significant, and these models should be more effec-
tively incorporated into the risk assessment through
revisions to Chapter 8.

4.1.3  Incremental Exposures and
       Bioaccumulation  (Charge Ques-
       tion 4)

       The  EPA  reassessment  document
provides a large amount of animal and human
data regarding  bioaccumulation  of  2,3,7,8-
TCDD.  However, it is readily apparent that
a large gap exists in our knowledge regarding
the pharmacokinetics of common, environ-
mentally  occurring  congeners  of  2,3,7,8-
TCDD.   If  Toxicity  Equivalency  Factors

   ... the Committee anticipates
     that future revisions of the
   reassessment document will
   incorporate the latest data set
  from the  Ranch Hand study...
(TEF) are going to be used to assess human
toxicity, and if approximately 10% of the total
exposure  to dioxins is attributable to 2,3,7,8-
TCDD, an accurate estimation of total poten-
tial for toxicity  can be made only if informa-
tion is available regarding distribution, metab-
olism, and half-lives of other major contribut-
ing components (See discussion in  section
4.13).  It is not possible to make a  general
estimate of toxicity if we know nothing of the
pharmacokinetics of the   majority  of  the
dioxin-like compounds to which  humans are
exposed.   The reassessment is silent  on this
issue, but data from autopsy evaluations have
been  published by  Schecter  (1991) and
should be  used (if possible)  to extend our
knowledge  of the pharmacokinetics  of these
compounds.

     Major shortcomings are evident in the data
base and the relevant issues have not been dealt
with adequately.   For example, no attention has
been given to reviewing the half-lives  of many
of the important compounds  that constitute
the TEQ.   Data  from studies concerning
exposures of subsistence fishermen appear to
be in conflict with the EPA estimation of body
burden  (Columbia  River  Intertribal  Fish
Commission,  1993;  Dewailly et al,  1994;
Svensson et al.,  1991).  These studies suggest
that the reassessment's estimated body bur-
dens may be as  much as two orders-of-magni-
tude higher than actual levels; this difference
may be related to errors introduced by not
accounting for  the various  half-lives of the
agents included in the TEQ.

4.1.4  Uncertainties in Back Extrapola-
       tions of Body Burden (Charge
       Question 5)

     The Committee's primary concern with
the topic of the body burden back extrapola-
tion lies with EPA's treatment of uncertainty
regarding the half life of dioxin, and the expo-
sition of the methods for the back calculation
in the health document.

     As  discussed at the public meeting, the
Committee  anticipates that future revisions of
the reassessment document will incorporate
the latest  data  set from the Ranch  Hand
study, which should narrow considerably the
range of estimates for dioxin half-life, and so
reduce  the  uncertainty (from that source) in
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the back extrapolation of body burden.  In
addition, we expect that EPA will revise the
discussion on the back calculation method to
include material covered in Chapter 6 of the
exposure document, but  not currently ad-
dressed in the health document.

4.2 Mechanisms of Dioxin Action

4.2.1   Animal-to-Human Extrapolations
       of Receptor Structure and Func-
       tion (Charge Question 6)

       Mechanisms of dioxin action  are dis-
cussed in various parts of the Health Assess-
ment document.  Overall, the presentations
are very  useful.  Whether or not the docu-
ment addresses all  the relevant information
and alternatives depends, however,  on the
section that is being read.  Chapter 2 offers an
unequivocal assessment that "...the Ah recep-
tor mediates the biological effects of TCDD."
Yet, when reading Chapters 3, 4 and 5 dealing
with specific  toxic  events, it becomes clear
that  numerous  fundamental uncertainties
occur and mechanisms of action for the toxic
events beyond receptor binding are largely
unknown.  In Chapter 9, however, there is a
return to the acceptance presented in Chapter
2.  This  may  indicate that the authors of
Chapter 9 are willing to go along with the Ah
receptor information as it exists today.

       One major difficulty lies with the use
of the term "mediator" and perhaps confusion
about  what  constitutes  a  "mechanism of
action."  Other than what is described in the
sections dealing with  developmental  toxicity
(e.g., cleft palate, hydronephrosis), very little
is known about the biological steps  that fi-
nally lead to frank toxicity; the lack of infor-
mation on  immunotoxicity  is  particularly
limiting.  Much of what is purported to link
the Ah receptor  to  specific toxic events is
merely the  demonstration of an  association
between the binding of TCDD to the receptor
and  an eventual  appearance of an  adverse
effect some tune later in some species (when
the interspecies variation in doses that pro-
duce lethality can be over 8,000-fold, based
on the data provided in Table 3-1, Volume 1,
of the reassessment  document).   But  the
possible "downstream" events, if they exist,
between Ah  receptor binding and the final
toxic manifestation  are not well established.
"Mechanism of action" should mean that at
least some of the intermediate steps, after Ah
receptor binding and leading to the pathologic
processes involved, are known to some extent.
The loose use of the term "mediator" implies
that  the association apparently observed is in
reality the initiator of the process.  In actual
fact,  the only mechanism of action involving
the Ah receptor  that has been worked  out
sufficiently well  to  be called the  biological
sequence  that describes a  "mechanism  of
action' is the induction of cytochrome P450.
What is known about the induction process is
truly elegant.  The rest of the biological conse-
quences of TCDD exposure are yet to be  de-
scribed adequately and sequentially in mecha-
nistic terms.

     The document (Chapter 2) presents an
excellent review of what is known  about  the
Ah receptor and the multiple steps involved in
TCDD-induced cytochrome  P450 induction.
Research findings during the last 10 or so
years that have identified the structure and
mode of action of the Ah receptor represent
a  major  scientific  achievement.   How a
planar polar compound such as TCDD binds
with a cellular receptor, followed by translo-
cation into the nucleus, where transcriptional
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PAGE  42
activity of DNA is influenced, is very well
understood.  On the  other hand, there is a
large intellectual chasm between the elegant
science describing the details of the TCDD
receptor and its  mechanism of initiating a
cellular response, and the poorly understood
manifestations of the  toxic events associated
with an alteration of the homeostasis  of an
animal.  The linkage  between Ah receptor
action and specific cellular toxicity remains
undefined.  Several Members of the Commit-
tee noted  that the possibility that  the Ah
receptor system may be a sensing pathway to
protect the cell is not considered, nor are
there other attempts  to put forth scientifi-
cally  testable hypotheses.   In any  future
revisions,  EPA should present  more clearly
the major  deficiencies that exist  in the cur-
rent mechanism database and provide  some
discussion  of any plausible alternative hy-
potheses.

       Although  the  Ah receptor is  likely
involved in producing TCDD toxic effects of
potential  concern  to  humans,   there are
multiple levels of regulation of the receptor
pathway.   The  induction  of CYP1A1  does
not serve as a good model for all receptor-
mediated  responses  to  dioxin, particularly
those  that result  in  altered patterns of
growth and differentiation. The  studies of
Poland and  Knutson  (1982b)  in  hairless
mice  indicated that  for responses such as
epidermal   hyperkeratinization  and   skin
tumor promoting activity, the Ah receptor is
necessary, but not sufficient.  Two implications
from  these  studies are: a) that  toxicity  is
under multiple genetic control, and b) the
most sensitive response in animal models is
not necessarily the most valid predictor of
toxicitv in  humans.  Chloracne remains the
      ^
most   definitive  response documented in
humans and clearly occurs at high exposure
levels.   However, there are  relatively few
animal models for chloracne.  Most  of the
"mechanistic data" support the involvement
of the Ah receptor, but say little (in the
context of toxicity), about how the activation
of this  protein  alters   normal physiologic
function  and/or development.  Risk  assess-
ments based solely, on Ah receptor activation
or on  the existing knowledge of CYP1A1
induction are unlikely  to provide a biologi-
cally defensible prediction (quantitatively or
qualitatively)  of likely  toxic  outcomes in
humans,   particularly under  low  exposure
scenarios.

     Regarding the Ah receptor in humans,
there is adequate evidence  that, in  overall
function,  the human Ah receptor mechanism
essentially acts the same as  the Ah receptor
in rodents,and in other laboratory species.
The Ah receptor in humans, however, has an

 The linkage between Ah receptor
     action and specific cellular
     toxicity remains undefined.
affinity  for  TCDD that is  lower  than the
affinity in C57BL/6 mice or in most labora-
tory rat  strains.  There may be at least  a
10-fold  range of variation  among  the hu-
man population in the  affinity with  which
the Ah receptor binds TCDD.  Induction of
CYP1A1 exhibits classical sigmoidal log-dose
response  curves in several human cell lines
in culture.

     The  EPA document's  phrase  "...and
the role  that the  purported  mechanisms of
action might  contribute to  the diversity of
biological  response seen in  animals and, to
some extent,  in humans?" in this question
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can be  better posed as "...how convincing is
the evidence for the purported mechanisms
that link receptor binding to toxic effects in
humans?"   Unfortunately,  the evidence is
quite  mixed.  There is only  limited evidence
for toxic effects  in the Ranch  Hand study
(USAF, 1991;  CDC,  1988a,b).   Studies of
the Seveso population  (Bertazzi et al., 1993;
Bertazzi et al,  1989; Mocarelli et al, 1986)
show  significant excesses of multiple  my-
eloma  and  hepatobilliary  tract  cancer  in
women, and lymphoreticulosarcoma  in men
in zone B,  and soft tissue  sarcoma  in men
in zone  R.   These lesions, however, were
seen  in  the zones of lower,  not  higher,
estimated exposure.  Conversely, and  further
clouding the issue,  these  same studies  also
report decreases in breast8  and  other  cancers
in females (although the number  of  cases is
very  small and the decrease may be spuri-
ous).   In the exposed chemical plant work-
ers studied by  the  National  Institute  of
Occupational Safety and  Health  (NIOSH)
(Fingerhut et al,  1991), there  is no excess
cancer of any  kind in  the  less  heavily  ex-
posed workers  (i.e., those who  were exposed
for less than one year). Workers with over
one   year's  exposure  showed  statistically
significant  increases in respiratory  system
cancer,  but they were  exposed to  a wide
variety of potentially  carcinogenic agents in
addition  to  dioxin.   Given  the  possible
confounding, and  the somewhat  equivocal
links    of   dioxin   to    excess   cancer
in the group  as a whole, it is  difficult  to
document a dioxin-cancer relationship.9 10

4.3   Toxic Effects of Dioxin

4.3.1   Animal Models for estimating
        Human Risk (Charge Question 7)

      The Committee believes that the EPA reas-
sessment document reviewed  and  summarized
adequately the current knowledge base on the experi-
mental disease(s) produced by TCDD and related
compounds in animals,

      The Committee  agreed that,  because
there is a limited amount of human data, EPA
will  have  to rely on the results  of animal
studies for some portion of its risk character-
ization. However, the  Committee also finds
that it is  probably not appropriate  for the
Agency to  single out the results of a given
study  (unless  it is of a seminal  nature)  for
decision making. The  EPA would be better
served  to employ a weight-of-the-evidence
approach, using the totality of the data.
          Some Committee Members suggest that this
reduction may result from the anti-estrogenic effects of
dioxin.
          Several Members of the Committee suggest that
EPA has neglected other human exposure studies of interest,
particularly the health effects seen in the Taiwan rice oil PCB
poisoning episode (as reported in Rogan et al., 1988; Wu et
al., 1984; Chang it al, 1982a; Chang et al, 1982b: and
Chang et al, 1981) which could reduce the reliance on
animal-to-man extrapolation.


           Noting that cancer excesses were seen in the
Fingerhut et al. (1990; 1991) studies only in workers with 20
or more years of latency, one Committee Member argues that
the Bertazzi study should be discounted as uninformative. It
was carried out only ten years after exposure, the levels of ex-
posure to the people in the zones that are characterized as
having excess cancers are far less than those of the workers
studied by Fingerhut et al. In this Member's opinion, both
the reported excesses and deficits are more likely to result
from chance than from any dioxin effect.
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PAGE  44
       The  Committee noted that although
there were many interspecies consistencies in
response  following  exposure to  the  com-
pounds in question, there were also many
significant inconsistencies.  The Committee
questions whether some  of the interspecies
differences were "true" differences or were a
reflection of  the dose  levels  used  in  the
different  studies.     A   portion  of   the
interspecies  Inconsistencies  may  be attrib-
uted to  the fact  that some of the  animal
studies involved  lethal exposure  levels but
that other  studies did not.   For example,
some  of  the  effects  noted  in  animals  (see
Table  9-2 of the  reassessment document),
e.g.  "wasting  disease," chloracne,  testicular
atrophy,  hepatotoxicity,   cardiovascular  le-
sions,  hypoglycemia, edema, and  porphyria,
were found primarily in  animals  that  died.
Many  of these effects were  not observed in
cows, however, and none  of the cattle stud-
ies  involved lethal exposure levels.  There-
fore, the  lack of interspecies comparability
may indeed  be a reflection of the dose levels
used in particular species/studies.

       Another apparent inconsistency which
may be  a reflection of study  design is re-
lated  to the carcinogenicity  of  TCDD.
Although TCDD  has not been reported to
be  carcinogenic   in  guinea pigs,  rabbits,
chickens,  cows,  or monkeys, none  of  the
studies were of sufficient length to ascertain
whether it  is  indeed carcinogenic  or not in
these  species.   Additionally,  the lack  of
observed chloracne in a given species may be
related to anatomical  differences; many of
the tested species have "fur"  rather than true
"hair"  follicles as are found  in monkeys and
humans.  Also, a feather  follicle (chicken) is
anatomically  quite  different  than  a  hair
follicle.   Some  discussion  of  these  points
would be beneficial to readers of the reas-
sessment document.

      The Committee also suggests that the
document discuss "primary" versus "secondary"
effects of exposure. For example, some of the
effects related to TCDD exposure (testicular
atrophy, cardiovascular pathology, edema, and
porphyria)  may not be directly related to the
compound, but may rather be a reflection of
the "sick  animal"  syndrome.  Comparative
mechanistic arid pharmacokinetic data would
be needed before making a conclusion that a
given effect is directly attributable to TCDD
or related compounds.  The degree of uncer-
tainty in this regard needs to be discussed in
the document and needs to be reflected by
using "?" marks in Table 9-2.

      The Committee believes that Table 9-2
is extremely important and is very likely to be
used  by the casual reader; consequently it
should  be   made   as accurate  as  possible.
Members of the Committee have noted errors
in the Table, and we suggest that the tabular
material in the document  be reviewed  for
accuracy.

      In summary, the Committee finds that there
is  evidence of both  inter-species  consistency and
inconsistency in the EPA  document.  These vari-
ances need to be addressed in an objective manner in
the document, and discussed in conjunction with all
the uncertainties inherent in the various endpoints.
In addition,  the Committee felt strongly that an
examination of the totality of the animal data will
be required in the final hazard characterization.

      Finally, nearly all of the Members of the
Committee take strong exception to the defin-
itive sentence on page 9-78 (see also the Com-
mittee's discussion of Charge Question 1, in
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                                                                              PAGE   45
section 2.2.2 of this report) of the EPA docu-
ment, which states:

"The scientific community has identified and
described a series of common biological steps
that are necessary for most If not all of the
                j
observed  effects of dioxin and  related com-
pounds  in vertebrates,  including  humans.
Binding  of dioxin-like  compounds to the
cellular protein Ah receptor represents the
first step in the series of events attributable to
exposure to dioxin-like compounds, including
biochemical, cellular, and tissue-level changes
in normal biological processes. Binding to the
Ah receptor appears to be necessary  for  all
well studied effects of dioxin but is not suffi-
cient,  in and of Itself,  to elicit these re-
sponses."

This pronouncement is too strong.  Virtually
all the Committee believes that it is more accurate to
state that binding of TCDD and related compounds
to the Ah receptor is a marker of exposure, but has
not yet been established to be  "necessary" for the
induction  of several  of the observed effects.  This
degree of uncertainty needs to be stated in the
document.

4.3.2  Variations in  Human Sensitiv-
       ity  (Charge Question 8)

       The issue of human sensitivity may  be
divided into two questions.  First, are human
sensitivities so distributed that a  representa-
tive average can be assumed? Secondly,  do
humans  on average yield a response which
might be considered to  be average relative  to
the spectrum of responses in animals?

       Wide variations  in response to dioxins
are well documented in animal studies, with
at least a three-fold order-of-magnitude being
reported for some responses between animal
species and even within a given animal species
such as rats and mice, or between very young
hamsters  and adult hamsters. Responses of
humans are known to vary by several orders-
of-magnitude with respect to the exposure to
many exogenous substances as drugs, where
some individuals  are known to be responders
while others are  considered non-responders.
It is reasonable  to assume,  therefore,  that
responses  of humans to TCDD and its conge-
ners will vary widely.  Furthermore, observa-
tions reported in human  exposure  studies as
from Seveso, Italy (Bertazzi et al.,  1993;
Bertazzi et al.,  1989; Mocarelli et al.,  1986)
and the U.S. Air Force  Ranch  Hand Study
(USAF, 1991; CDC,  1988a,b)  as well as other
studies  of occupational cohorts clearly indi-
cate that wide variations in the frequency and
severity of response occur.''

     The Committee finds that there is no
single  animal model that  could accurately
predict human responses.  EPA, in its revision,
should  identify  clearly   the  limitations  of
existing animal models in terms of their abil-
ity to predict the various health outcomes that
may occur in humans as a result of exposure
to dioxin and  dioxin-like compounds.  Are
humans expected to yield an average response
relative to the spectrum  of  responses in ani-
mals?  Humans could be as sensitive  as  the
most sensitive mouse species, or insensitive as
the least  sensitive mouse species to TCDD.
Based  on the  available  human data, it is
debatable whether the most sensitive species,
          One Member of the Committee objects to this
statement, citing findings in this review which assert that the
only adverse human health effect tied to dioxin is chloracne.
Given this latter finding, he does not accept that studies
"dearly indicate that wide variations in the frequency and
severity of response occur."
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PAGE  46
or  the most representative  animal species,
should be used when  selecting an animal
model to predict TCDD toxicity in  humans.
Ideally, if a high degree of confidence in the
model existed,  one  should use  the animal
species that is most representative of humans.
If no  single  model  is  appropriate, animal
models should be selected that permit a con-
servative  approach to be employed with re-
spect to extrapolation to human subjects.

       What is unclear with respect  to the
question regarding average sensitivity is what
constitutes an average response in humans who
exhibit an average sensitivity, and at what level
of exposure, both acute and chronic, does this
occur?

       When considering toxicity to humans,
one must always consider the most  sensitive
population.  Can highly sensitive sub-popula-
tions be identified that are at greatest risk to
dioxin  exposure? Such sub-populations might
include pregnant women,  infants,  and  chil-
dren or members of a population  with an
above  average exposure as populations whose
subsistence diet consists largely of fish.

       Although variations in response are reported
and presented in the document, the document (in the
opinion of most of the  Committee) does not (and
perhaps cannot, given  that dioxin-specific effects
beyond  chloracne—see the discussion below—are not
established) concisely articulate that wide variations
in human  sensitivity to dioxin exposure occur and
should  be  anticipated.   The  emphasis is  heavily
placed  on  low level exposures that might cause
toxicity in some individuals.12
4.4  Chloracne as an Indicator of
      Exposure  (Charge Question 9)

      The Committee believes that the EPA docu-.
ment reflected adequately the current knowledge base
on chloracne as it relates to the subject compounds.
Chloracne is a ckar indicator of exposure, but the
absence of chloracne in an exposed subject is not an
indicator of low exposure. In fact, the Commit-
tee's consensus is that chloracne  is the only
lesion of note  clearly established as being
related to TCDD exposure; in the absence of
sufficient data on human tissue levels at peak
development  of chloracne, however,  a dose-
response relationship is difficult to ascertain.13
The Committee also noted that chloracne has
also been found in people exposed to related
compounds such as dibenzofurans and PCBs.

4.5   Cancer

4.5.1   Epidemiological Evidence
        (Charge Question 10)

      When a difference between compared
groups is observed, "causation" may still not
be imputed.  Similarly, when no difference is
seen, it  cannot be concluded that the study
variable is not associated (noting, of course,
that  the presence of "association" does not
impute  causation) with some outcome or is
not "causal." Causation is not itself an experi-
mental or epidemiological result but a judg-
ment made about  the results.  In making such
a  judgment  an  epidemiologist takes  into
account the possibility that bias and chance
       12
          Several Members have noted that they find the
EPA emphasis on low-level exposures to be appropriate and
consistent with prudent public health practice.
           Several Members of the Committee believe
that recent research findings published after the Committee's
public meeting (e.g. Kogevinas et al. 1995; Huisman et al.,
1995) establish some cancer and developmental effects in
humans as outcomes of either CDD or TCDD exposures.
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                                                                               PAGE  47
may play a part, but additionally examines the
observed association in  terms  of  certain
characteristics  associated with  "causal" rela-
tionships. These are sometimes  referred to as
the  Hill criteria  (for A. Bradford Hill, who
first codified them). They are: a) strength of
the association; b) consistency;  c) specificity;
d) relationship witJh time; e) biological gradi-
ent (dose-response relationship); f) biological
plausibility; g)  coherence of the evidence; h)
observed change following some intervention;
and  i) analogous  findings.

       The reassessment document mentions
three  of the Hill criteria (gradient,  consis-
tency, and strength) but does  not explicitly
use them as a  tool to organize  its discussion
of causality.  The Committee does not regard
this as a failing, since the Hill criteria  are not
so much "rules," as viewpoints to  aid in
interpretation.   The  EPA response  is that
causation judgments were  not explicitly part
of Chapter 7, although the Committee notes
conclusions  of Chapter  7 were used as  a
partial basis for Chapter 9.   We find  this
acceptable, since  the entire discussion is couched in
terms of characteristics of causal associations.

       Understanding the operation  of bias
and  chance is  especially important in inter-
preting so called "negative  studies"  (studies
where no differences are apparent, or where
the  differences are  not "statistically  signifi-
cant").  Differences produced by real effects
can easily be masked by poor exposure classi-
fications (misclassification bias), Chance  can
appear as a possible explanation merely by
virtue of a  small  population  available  for
study  (poor statistical power), and potential
risks  can be undetectable by observing  the
exposed population for too short a time (bias
produced by failure to account for adequate
latency), to name just a few factors compli-
cating interpretation of such outcomes.  On
the  other  hand,  factors that  can produce
spurious  increases  in  exposed  groups  in
environmental  epidemiological  studies  are
much less common, most forces operating to
lower the observed risks, not raise them.  The
reassessment  document does a  quite good  job of
taking  these  limitations into account,  but could
still benefit from additional discussion of the effects
of confounding factors.  Specifically, EPA should
incorporate in a  revised Chapter 9 the means
of addressing confounding  factors discussed
by Agency staff at the review meeting.

      To  summarize  the  above  discussion,
evaluating  internal  validity  requires  the
assessment  of  the  roles  played  by  bias,
chance and real effect.  Each  can operate,
sometimes reinforcing other factors, some-
times offsetting  other factors. There is often
disagreement  about  studies  among experts,
stemming from  differing weights each places
on  the influence of bias,  chance  and real
effect.  Such  differences in science  are com-
mon,  both  in  and  out of  the regulatory
process.   The Assessment  is explicit about
the judgments it makes, allowing others to
differ if they feel  that other emphases  are
warranted.   The  Committee  feels this is
preferable to merely cataloging variant points
of view.

      An evaluation of internal validity helps
a scientist in  deciding how much to rely on
the specific result of the experiment or study.
It does  not  tell a  scientist  how much  to
extend that result to contexts  or situations
different than  the one  studied,  i.e., how
much  to generalize  the result.   A separate
evaluation for external validity is needed.
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       The  limits and extent of generaliza-
tion are given by a study's external validity.
In  our context the question is whether a
proposition developed in one context (e.g., a
high  dose  occupational  study like that of
Fingerhut et al.,  1990) can be generalized to
cover  other  contexts  (e.g.,  environmental
exposures).  Unfortunately, there are no rules
for how far  to  generalize,  if at all.   Each
study must be evaluated in a specific context
to determine the extent to  which it can be
generalized.

       Cross-species generalization is not a
problem for the epidemiological studies, but
the question of generalizing to environmental
doses remains.  Interestingly, Chapter 7A of
the Assessment (Epidemiological Data,  Part
A Cancer Effects) does not  comment on the
applicability  of the epidemiologic data to
environmental  exposures,  satisfying  itself
with  answering  the  question  of whether
TCDD and  related  compounds  have the
capacity to cause cancer in humans under
any conditions.  It answers in the affirmative,
citing  several studies of "sound design and
adequate size" that have found a risk of soft
tissue sarcoma (STS)  (p. 7-74).  Association
of STS with dioxin exposure was raised by
Hardell and colleagues (Hardell et al, 1979;
Hardell,  1981a,b; Hardell and Eriksson,
1988; Hardell, 1993)  and,  according to the
reassessment,  has  "stood  up • to extensive
criticism and a  great  deal of  subsequent
research." (pp. 7-73,  7-74).  The document
also notes that the entirety of the association
in these studies may be"real" and not "due
to selection bias,  differential exposure  mis-
classification,   confounding,  or   chance."
Although there are differing opinions about
the validity  of the Swedish studies,  most
Members of the  Committee find that the
Assessment  clearly  discusses  the  direction
and  degree  of  influence  of the  various
sources of bias in these studies.

     The  reassessment  similarly  discusses
non-Hodgkin's Lymphoma (NHL) and, based
upon a  lack  of a "minimally consistent pic-
ture  of  increased risks" fails to conclude at
this  time  that dioxin exposure is  related to
NHL.  This  conclusion was arrived at by a
clearly  stated  and  scientifically  defensible
argument  that is adequately supported.

     The reassessment also states that  the
epidemiologic data  suggest that lung cancer
might also be related to dioxin exposure, and
cites  the  findings   of  the  NIOSH  study
(Fingerhut et  al.,   1991)  which  reported
statistically  significant increases  in respira-
tory system cancer  (for those  with over one
year's exposure and a 20 year latency period.
Because of possible uncontrolled confounding
by smoking,  as well as by exposure to many
other carcinogens in the workplace, the EPA
document is more  tentative  on  this judg-
ment, but considers that residual  confound-
ing  is  insufficient to  explain  the  observed
increase in  respiratory system cancer risk.
Here the basis for the  EPA's position  is
weaker, reflecting the current lack of data  on
the  possible  nature  of a  dioxin/cancer rela-
tionship in the presence of confounding  by
smoking and  occupational exposure to chem-
icals.  Although the  Committee does  not
reject  the EPA's position, neither  can  it
reject  the alternative  explanations  which
some Members of the Committee think have
more merit.

      The document found insufficient data
to  make   conclusions  regarding  stomach
cancer, generally increased risk of all types of
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                                                                               PAGE   49
cancer, and sex differences  in  cancer risk.
The Committee agrees.

       In summary,  almost all  Members of
the Committee found that the reassessment's
judgments on the epidemiology data (subject
to the caveats noted) to be generally defensi-
ble.  The document took into account many
of the concerns of the broad scientific com-
munity and discussed them explicitly, but, of
course, could not discuss all significant alter-
native viewpoints.14

       The  Committee   does  have  some
concerns  about the ways  and the extent to
which uncertainties  in the  epidemiological
data base were characterized. The reassess-
ment document (p.  7-77) refers to "uncertain-
ties  associated with the  epidemiologic  evi-
dence."  Some of these uncertainties are the
usual ones  attendant upon a subject  where
much research remains to be done and many
questions are  still  unanswered.   The more
important uncertainties are those connected
with  the epidemiologic method itself.   Ob-
serving some unintended or "natural" experi-
ment in the real world, which is the essence
of observational studies like epidemiology,
has the enormous advantage that it involves
human beings living under conditions similar
to ones of concern to regulators and  public
health officials.  For epidemiology, the  uncer-
tainties are largely associated with the ques-
tions of internal validity extensively discussed
in the Assessment itself (questions  of bias,
chance and real effect).  The reassessment
document did not discuss  remaining ques-
tions of external validity, the most important
of which are the high exposure to low expo-
sure generalization.  The EPA should  com-
ment on this issue in any future revision,  as
well as on the relationships between agricul-
tural and forestry, and  environmental expo-
sure levels (as well as varying exposure routes
and patterns and associated  environmental
conditions and chemicals in these situations)
and the cancers  observed at those exposure
levels.15

4.5.2   Carcinogenicity of Dioxin-like
        Compounds  (Charge Question
        11)

      TCDD is one member of a large family
of  halogenated  polycyclic  aromatic   com-
pounds.   The EPA  reassessment  document
describes  such congeners and some of their
metabolic and carcinogenic  effects.  A num-
ber  of other studies  have demonstrated the
effectiveness  of  other  dibenzodioxins and
dibenzofurans,  both individually  and   in
mixtures, as promoting agents  in the rat liver
model (e.g.  Nishizumi  and Masuda,  1986;
Waern et  al, 1991; Shrenk  et al,  1994).
Although the  data is as  yet too sparse  to
make  any  extensive generalizations,  it  is
reasonable to hypothesize that many of these
congeners of TCDD will be effective promot-
ing  agents  and  thus carcinogenic at some
dose.
       14
          Likewise, the Committee did not discuss in its
public meeting, or address in this report, all the relevant
epidemiological studies noted in the reassessment document
or the extant literature.
          One Member of the Committee notes that the
reported effects of low-level exposure to forestry and
agricultural workers predicts overwhelming incidence of
certain tumors in the far more highly exposed production
workers. Those tumors are not in excess or are barely in
excess (see above) in the production workers. At least, this
absence of external validation casts doubt on the general-
ization of the forestry and agricultural studies. At worst, they
indicate that those studies are flawed.
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       Another large class of closely related
halogenated aromatic hydrocarbons are the
polyhalogenated  biphenyls.   Many of  the
members of this class are promoting agents in
the rat liver system (Sargent  et al.,  1991;
Jensen  and Sleight,  1986;  Luebeck et  al.,
1991; Preston et al., 1985) and in other organ
systems as well (Anderson et al.,  1994).   In
addition many, but not all, polyhalogenated
biphenyls interact specifically  with  the  Ah
receptor (Kafafi et al., 1993) at KD  (the appar-
ent equilibrium   disassociation  constant  for
ligand binding to  the Ah receptor) levels that
approach that of TCDD.  However, the ma-
jority of the Committee concluded that  the
structure, metabolism,  gene regulation and
toxicities of this class, while overlapping with
some  of the characteristics of dibenzodioxins
and  dibenzofurans  (Safe,  1990)  are  suffi-
ciently different from those  of the latter to
argue that polyhalogenated biphenyls not be
a part of this  document.16  They may be
considered for future studies on assessments
using this document as a model.

       Since  many  dibenzodioxins   and
dibenzofurans occur as  components  of mix-
tures, there have been several studies of  the
carcinogenicity of such mixtures indicating
additivity of their promoting activity (Schrenk
et al, 1994; Huff et  al., 1991).  In some in-
stances synergy of components of mixtures of
biphenyls have been reported (Sargent et al.,
1991; 1992).

      Although the  discovery and character-
ization of the Ah receptor were delineated
using polycyclic hydrocarbons (halogenated or
not) as  ligands   (Thorgeirsson  and  Nebert,
       16
          Several Members disagree strongly with this
finding and recommend the inclusion of polyhalogenated
biphenyls in the assessment.
1977), it has been  assumed  that  naturally
occurring and/or endogenous  ligands occur.
Recent studies (Bjeldanes et al., 1991) identi-
fied some naturally occurring indoles as effec-
tive ligands.   However, the contribution of
such agents to the "dioxin burden" as ligands
of the Ah receptor is unknown. Clearly more
studies in this area are needed.

     In    summary,   dibenzodioxins   and
dibenzofurans which have been studied as
congeners  of  TCDD, exhibit  qualitatively
similar toxicities, ligand reactivity with the Ah
receptor, and show carcinogenic potential as
promoting agents. However, the data in this
field are  too incomplete at this time to make
generalizations in the direct  application of
findings with one member of  the class, e.g.,
TCDD, to all others.  Promoting activity of
mixtures  of this class can be additive and, in
some cases, may be synergistic. The Commit-
tee (albeit with several Members taking excep-
tion) recommends that the  polyhalogenated
biphenyls,  although having many similarities
in their  metabolic,  toxic,  and  carcinogenic
effects  to TCDD, should be considered in a
separate  class and not considered in depth in
this document.

4.5.3  Carcinogenic Activities of Dioxin
       and Dioxin-Like Compounds
       (Charge Question 12)

     The  EPA  reassessment  document, in
describing the experimental evidence, makes
a clear distinction between studies that imply
TCDD as a multi-site, complete carcinogen, as
opposed  to studies that emphasize the pro-
moting properties of the agent.  The principal
animal assays that evaluated the carcinogenic
potential of TCDD and some of its congeners
are adequately reviewed.  On occasion, how-
ever,  emphasis  is placed on  the  fact that
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                                                                                  PAGE  51
     TCDD is carcinogenic well below the maxi-
     mum  tolerated doses; what should  also be
     noted  is that quite often the response was
     statistically not significant (e.g., skin tumors in
     hamsters (Rao etal, 1988) and liver tumors in
     mice (Sugar rt a/., 1979)).

            In the document, the classification of TCDD
     as a complete carcinogen is done on an operational,
     not mechanistic basis.  This is confusing since the
     classical definition  of a complete carcinogen
     necessarily involves a consideration of  the
     mechanism(s) whereby the agent effects its
     action. The  term "complete carcinogen"  has
     been  reserved, until the  advent of this and
     other recent documents on the carcinogenicity
     of TCDD (Huff et al, 1994), for agents capa-
     ble of inducing all  stages of cancer develop-
     ment,  initiation, promotion, and progression
     (Boyland,  1980; Pitot,  1990).   TCDD, as
     documented in the reassessment (as  well as
     from other sources), is incapable of initiating
     cells in multiple in vitro and in vivo studies, and
     has never been satisfactorily demonstrated to
     have progressor activity.   It  should  not be
     classified as a complete carcinogen any more
     than phenobarbital, phorbol esters, uracil or
     galactosamine would be considered  as com-
     plete carcinogens. Thus, if the term "complete
     carcinogen" is to be  retained as a classification
     of the carcinogenic action  of TCDD, a  full
     definition of the term as used in the document
     must be given to prevent the confusion noted
     above.  Furthermore, designation as a complete
     carcinogen implies in the minds of most read-
     ers direct mutational and clastogenic  activity
     of the agent, which the evidence  does  not
     support for TCDD.

            The  reassessment document  also  de-
     scribes a second set of studies in which TCDD
     was characterized as having  promoting  capa-
     bilities. TCDD is classified as an  "... extraordi-
narily  strong promoter of  liver  and  skin
tumors."   The  following studies  are  refer-
enced in the EPA document as support for
this statement:

a) Liver studies:

  1)  Liver-tumor   promotion   by  TCDD
     following  initiating  treatment   with
     partial     hepatectomy/DEN     (Di-
     ethylnitrosamine)was first described in
      1980 (Pitot et al., 1980).   In female
     adult Charles River rats treated with a
     high  dose of TCDD following partial
     hepatectomy, five out of seven eventu-
     ally developed liver  tumors vs.  none
     out of  four in controls.  This was ac-
     companied by an increase of enzyme-
     altered foci.   No effects  were  seen in
     five animals treated with a low dose of
     TCDD.   The  findings are significant
     when a one-tailed Fisher's exact test is
     applied.

  2)  In  another  study with  adult female
     rats, a single dose of DEN was used as
     initiator and TCDD as  a promoting
     agent (Graham  et al,  1988).    This
     study also suffers from low  statistical
     power,  and tumor data,  at 60 weeks,
     are only available for one  (the highest)
     out of three  TCDD doses studied (5/8
     vs. 1/5  in  controls).  According to the
     authors, "The number  of animals was
     not sufficient to determine whether the
     incidence  in  DEN  initiated/TCDD-
     promoted  rats was different  from that
     seen  in rats treated with  DEN alone."
     (A significant effect was claimed when
     DEN/TCDD-treated    animals    were
     compared  to animals  that  had  not
     received DEN -  clearly an inappropri-
     ate comparison).
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 3)    A third study (referenced several times
       in the  EPA document) was not pub-
       lished in the  peer-reviewed literature
       (Clark et al, 1991) and the complete
       data do not appear to have been pub-
       lished elsewhere. The report should be
       specific in this regard.  Data on tumor
       incidence are only given for intact and
       for  ovariectornized  animals  initiated
       with DEN and treated with TCDD;
       apparently only one TCDD dose was
       used.   No control data (rats initiated
       with DEN and treated with solvent)
       are  presented.  "When the available
       data on liver tumor incidence are ana-
       lyzed  by Fisher's Exact test, they do
       not support the assertion that ovariec-
       tomy would provide  a  "protective ef-
       fect" against liver tumor development.
       However, analysis of the same data
       with an uncorrected chi-square test,
       which is more appropriate for such an
       application (D'Agostino et al.,  1988)
       results in highly  significant support
       (p<.01) for the hypothesis of a mitiga-
       tion of effect in ovariectornized rats.

 4)    Another study has become available in
       the open literature since preparation
       of the EPA  reassessment document
       (Sills et al., 1994).  The group sizes of
       animals used  in this particular study
       provide better statistical power  (be-
       tween  12 and 15 animals per  group)
       than do the previous studies. Despite
       the larger numbers, the increased tu-
       mor incidence  in  initiated-TCDD
       treated female weanling rats (five  out
       of 15 animals) is not sufficiently high
       to demonstrate a  difference from  the
       controls (1 out of  12  animals) at  a
       conventional level of statistical signifi-
       cance.
      It  is interesting to note  that, taken
individually, two of the  four studies  on rat
liver  tumor promotion  by TCDD  cannot
demonstrate a statistically significant effect, a
point that should be addressed in the docu-
ment. However, in all studies,  there is evi-
dence of a TCDD effect and, if the data from
the four  studies are pooled using appropriate
statistical methodologies, the promoting effect
of TCDD becomes highly significant.

      Furthermore, there is a substantial data
base  wherein, although  no direct data on
tumor incidence or multiplicity are provided,
convincing evidence is given that treatment of
initiated animals with TCDD increases signifi-
cantly, and in  a dose-dependent  manner, the
presence of altered  hepatic foci and other
signs suggestive of a  strong promoting poten-
tial (Sills et  al,  1994;  Pitot et al.,  1987;
Dragan et al,  1992; Buchmann  et al, 1994;
Flodstrom et al, 1991; Waemrta/., 1991).  A
recent and  extensive  study on  the topic
(Dragan etal, 1992) emphasizes the complex-
ity  of this particular  endpoint.    The EPA
document describes several mechanistic stud-
ies and attempts are made to link the promot-
ing activity of TCDD to such  biochemical
events as enzyme induction, internalization of
EGFR, estrogen receptors, and  similar end-
points.  A recent study on inhibition of inter-
cellular communication by TCDD might be
added to this discussion  (DeHaan  et al,
1994).

b) Skin studies:

      Promoting activity of TCDD was also
shown in two skin studies:

  1)   In 1982, TCDD was described as pro-
      moting  skin  tumor development  in
      hairless  mice  (Poland et  al,  1982a).
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                                                                            PAGE   53
       The data presented in this paper fully
       support the assertion that TCDD is a
       skin tumor promoter.

 2)    A second experiment (Hebert et al.,
       1990)  claimed to confirm  some of
       Poland's observations, but when read
       carefully  actually did not quite do so.
       The table reporting the incidence of
       proliferative lesions does not indicate
       statistically significant observations.  If
       the data  are analyzed, only the num-
       ber of mice with papillomas  in the
       lowest TCDD  group is  statistically
       higher than in controls.   This is in
       contrast to the description of the data
       given by the authors: "With the excep-
       tion of the lowest-dose TCDD group,
       all mice  initiated with MNNG and
       treated with  promoters  had  an  in-
       creased number of  papillomas  and
       nodules"  (p.  366).   The document
       should be corrected.  Significant skin
       tumor-promoting responses were seen
       with the congeners PCDF and HCDF,
       but there was a paradoxical dose-effect
       relationship  throughout  (e.g., lower
       doses  produce higher responses), and
       this anomaly should also be pointed
       out.

       One aspect of this particular study is
incorrectly represented in the EPA document.
On page 6-23 of the EPA document, line 4-8,
it is stated:

"Results (of the  Hebert study) demonstrated
that 2,3,4,7,8-CDF was 0.2 to 0.4 times as
potent as TCDD and that 1,2,3,4,7,8-CDF
was 0.08 to  0.16 times as potent as TCDD.
These data suggest that the tumor-promoting
potencies of  structural analogues of TCDD,
like  the  promotion  of liver  tumors,  reflect
relative binding properties of the Ah recep-
tor."

This statement is in contrast to what Hebert
et al.  (op cit.) said about  the same data (p.
366):  "The  lack of  a clear  dose-response
makes it impossible to compare the relative
potencies of the three compounds as promot-
ers or to comment on the validity of the TEF
                              j
approach for promotion as an endpoint."

      The potency estimates of 0.2 to 0.4 or
0.08  to 0.16,  attributed  mistakenly  in  the
EPA  document to  promoting  activity,  are
derived from  a comparison of the  relative
changes  in  body weight and  organ/body
weight ratios  (op cit. p.  372).  This error
should be corrected in the revised document.

c) Lung tumors:

      The claim that TCDD  promotes lung
tumors is based on one experiment in which it
was found that in ovariectomized rats, treated
with  DEN and given TCDD,  lung tumors
developed (four in  39  rats), whereas none
were found in 37 intact animals treated with
DEN  and TCDD (Clark  et al., 1991).   No
data on animals treated with DEN alone  are
available. The study was not published in the
open literature (which should be noted in the
document).  The effect is  statistically signifi-
cant however, when analyzed with the appro-
priate test (uncorrected Chi-square).

      A second study on  the  promotion of
lung tumors in mice by TCDD  (Beebe et al.,
1995) reported that TCDD enhances tumor
multiplicity  in the lungs of mice treated with
DMN.   This study  needs to  be  discussed
within the larger context of the murine lung
tumor model being a representative system for
tumor promotion.
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PAGE  54
       Although  the EPA document provides a
good over-all view on the studies done on liver and
skin tumor promotion by TCDD, both  a more
critical analysis of the database and a more in-
depth  discussion of mechanisms underlying tumor
promotion, should be added,  Although some
investigators  believe that tumor promotion
must  result in neoplasms, the stage of promo-
tion, in fact, only involves the development of
preneoplastic  lesions  ranging  from enzyme
altered foci in rodent liver models to early
papillomas in multistage  epidermal carcino-
genesis in the  mouse.   In model systems
wherein the stage of promotion can be studied
independent of the stage of  progression, a
number of characteristics of the stage and of
the effects of promoting agents have been
delineated  (Yuspa  and  Poirier,   1988;
Rahmsdorf and Herrlich, 1990; Pitot,  1993).
Primary  among these characteristics  is  the
reversibility of the stage both  at the level of
gene expression and lesion growth. Promoting
agents themselves typically exert their effects
via receptor mechanisms and signal transduc-
tion.  Promoting agents in various systems in
vitro and in vivo inhibit programmed cell death
and apoptosis (Schulte-Hermann etal., 1991;
Magnuson et al, 1994; Wright et al,  1994).
Although there are some other promoter hallmarks
not yet established for TCDD, it conforms to all the
characteristics of promoting agents noted above, and
thus one is led to the conclusion that any carcino-
genic effect of prolonged TCDD exposure is primar-
ily, if not exclusively, the result of its action  as a
promoting agent.

       All of the evidence to date argues strongly
that TCDD exerts its carcinogenic effect primarily
through its effectiveness  as  a promoting agent,
stimulating cell replication in a reversible  manner
and inhibiting apoptosis, both mechanisms presum-
ably mediated  through the Ah receptor and associ-
ated transduction mechanisms.  TCDD is not a
complete carcinogen  and thus to avoid  confusion
should not be designated as such.   Many struc-
tural congeners of TCDD appear to act in a
similar manner to dioxin but there are as yet
insufficient data to make any generalizations
with respect to mechanisms of cartinogenesis
or toxicity for all such structurally related
chemicals.

      Finally, EPA needs to consider to what
extent data on female rat liver foci should be
used  in modeling the tumorigenic activity of
TCDD, be it as a promoting agent  or as an
"incomplete" carcinogen.  The ratio of foci to
tumors is far from being 1:1.  Many foci may
disappear when treatment is withdrawn;  on

   ... any carcinogenic effect of
   prolonged TCDD exposure is
  primarily, if not exclusively, the
       result of its action as a
	promoting agent.	
the other hand, it is impossible that foci can
grow to the  size  suggested by mathematical
models,  since one focus would occupy the
entire liver, a fact pointed out in the docu-
ment.  Attempts to incorporate  data into
models and  risk assessments will require a
critical in-depth  analysis of the biology of
altered hepatocyte foci.

4.5.4  Characterization of
       Dioxin/Dioxin-like Compounds as
       Human Carcinogens (Charge
       Question 13)

      Dioxin has been shown to  produce
malignancies in rats and mice of both sexes
Although the epidemiological evidence Unking
dioxin exposure to the genesis of malignant
neoplasms is limited,  and  does not  offer
compelling evidence  of carcinogenicity to
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                                                                              PAGE  55
humans when taken by itself, this evidence is
by no means inconsistent with such an effect.
Almost all  Members of the  Committee  therefore
concur  with  the judgment  that 2,3,7,8-TCDD,
under some  conditions  of exposure,  is  likely to
increase human cancer incidence.17 The conclusion
with respect  to dioxin-like compounds is less firm.
Since the information from animal studies is
much  less  robust,  and  that from  human
studies  often Limited by uncertainties about
exposure, the judgment depends wholly on
the similarity between the chemical effects of
            s
dioxin  and  those  of  its  congeners,   the
dibenzofurans and other related compounds.
For the congeners and dibenzofurans, enough
evidence  indicating  similarity  of  biologic
action exists to adopt the presumption that
they too are likely  to be  carcinogenic  to
humans under some conditions (although in
nearly all  instances  the doses  required  to
produce the same incidence would be higher
than those for dioxin).

       With respect  to the polyhalogenated
biphenyls  which  share  only  some  of  the
physical  characteristics  and  activities  of
dioxin, the  degree of carcinogenicity has not
been formally assessed in the present  EPA
document.    However,  at least  one  other
authoritative body, the International Agency
for Research on Cancer  (IARC), has  con-
ducted such a formal assessment and judged
both polychlorinated  (IARC,  1974; 1978a;
1987a) and polybrominated (IARC, 1978b;
1986;   1987b)  biphenyls  to be probable
human carcinogens,  both  on the basis  of
animal  studies  as  conclusive as  those for
dioxin, and, in the case of PBBs, with limited
       17
          Several Members contend that no epidemi-
ological study has produced evidence that is widely accepted
by the scientific community, including the IARC, as being
convincing for the human carcinogenicity of dioxin.
evidence from human studies.  The Commit-
tee did not dispute the IARC judgement that
PCBs and PBBs as likely  to  cause human
cancer under some conditions of exposure.

      The Committee agrees that assignment of the
dioxins, the PCBs, or PBBs to one of a mutually
exclusive and collectively exhaustive set of carcinoge-
nicity categories  considerably oversimplifies  the
state of the science in most  instances,  possibly
excepting  those compounds for which  there is an
abundance  of  uniformly  consistent  evidence.
However, prudent regulators must act and
cannot base their regulation on ambiguous or
inconsistent detail.  They must make  every
effort to treat  dangers of similar  magnitude
evenhandedly,  even  when there are limited
management options.  Although the  level of
exposure  and the potency  of the agent  are
measured on a meaningful continuous scale
and  can  be  incorporated  into  decisions on
the  basis of  unambiguous  continua,  the
degree of uncertainty re the human carcino-
genicity  of a compound is  not measured in
this  manner, but is  usually considered as a
categorical term.  It is desirable that consis-
tent criteria for this inevitable categorization
are employed.

      Under the proposed revisions to EPA's
guidelines, there are essentially three alterna-
tive  choices (unless "known to"  is  considered
an alternative to "likely to"):

a)    likely to  cause cancer under some con-
      ditions;
b)    not likely to cause cancer; and
c)    likelihood cannot be determined.

      In  Chapter 9 of the reassessment docu-
ment,  the basis for a detailed multidimen-
sional assessment is described and the vari-
ous  caveats  are underlined, but a choice
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PAGE  56  =
between  these  three  alternatives (actually,
between the first and the third-see below) is
still required.

       Under the  1986 EPA cancer guide-
lines, three more levels  of carcinogenic evi-
dence, with mutually exclusive  descriptive
terms  are provided,  giving regulators more
alternative  choices.  These choices  include
Group A -- human carcinogen; B,  —probable
human carcinogen on trie  basis  of limited
information  from human studies  as well  as
animal studies;  Group B: -- probable human
carcinogen on the basis of animal studies only;
Group C - possible human carcinogen; Group
D —  not classifiable; and Group E — evidence
of non-carcinogenicity for humans.

       In the case of dioxin, even if the addi-
tional alternatives were provided, virtually all
of the Committee believes that  the  animal
studies would be categorized  as  "sufficient"
and  the studies of humans as  "limited," pro-
viding for an  overall categorization  of Blf
which would be expressed verbally as "Proba-
bly  Carcinogenic to  humans with  limited
supporting information from human studies."
PBBs and PCBs would receive ratings of El
and B2, respectively.

       There is some merit in the provision of
a slightly more detailed set. of alternatives, but
the provision of Group  E, as  well as of the
proposed revised scheme  category of  "not
likely to cause  cancer"  may  be ill-advised.
Since all mechanisms of cartinogenesis are not
completely understood, and all sets of study
conditions  (species, dosage, co-carcinogens,
etc.) cannot be foreseen, it seems unwise  to
suggest that evidences of non-carcinogenicity
could ever be universally generalizable, at least
as worded (with no reservations).  Were one
to employ a restrictive phrase  such as "under
study conditions  judged to replicate most
recognized  conditions of human exposure,"
the class would be more defensible, although
one still would need to draw a difficult line
based on the conditions of the negative stud-
ies.

4.6   Developmental Toxicity and Ani-
      mal NOAELs (Charge Question 14)

      The specific issue of animal No Ob-
served Adverse Effect Levels (NOAEL), as it is
framed in the Charge, is inconsistent with the
question posed concerning the use  of the
Reference Dose (RiD) in evaluating incremen-

 . ..the animal studies would be
  categorized as "sufficient" and
      the studies of humans as
     "limited," providing for an
  overall categorization of B1...
tal exposures (see health question 18), and on
pages 9-69  ff of the reassessment document.
The latter question derives from the argument
that RfDs (which are based on NOAELs), are
inappropriate  for  the  current  assessment
because background levels may be significant.
If that argument is sound, why should it not
apply to animal models?

      Furthermore, even if the effect level
procedure could be defended for animals, is it
the optimal metric? The basis for Charge
question 18 is the reasoning that the proper
evaluation of risk in this context is the incre-
ment per arbitrary unit of exposure,  expressed
as some measure of body burden.  This is one
instance in  which dose-response modeling, as
by Benchmark doses, may confer a substantial
advantage.  What is gained by using NOAELs
and RfDs?   In a somewhat analogous situa-
tion-the relationship between lead exposure
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(defined as blood lead level) and IQ--the risk
is better expressed as 0.25 IQ points for each
l//g/dL than as  an RfD. This would be  a
more appropriate model for TCDD because,
as with lead, the location of a threshold for
developmental outcomes is rather uncertain.

       If,  for consistency with  traditional
EPA  practices,  a NOAEL were  to be  ex-
tracted from the animal data,  1 ng/kg day'
appears to lie in  an ambiguous zone. Murray
et  al. (1979), in a multi-generation study,
found that  10 ng per kg'1 per day"1  of TCDD
lowered the body weights and food consump-
tion of f 1 and f2 rats and also affected post-
natal survival. At 1 ng per kg"1 per day"1 both
increases and decreases were noted among
the survival indices of f 1 litters. A NOAEL of
1 ng per kg~l per day~l would be a reasonable
figure if based on these  data.

       On   the  basis  of  other  endpoints,
however, it may  not be as reasonable.  Mon-
keys whose mothers were undergoing  expo-
sure to TCDD in food (Schantz et al, 1989)
were  impaired,  relative  to  controls,  in  a
behavioral task called reversal learning. They
also displayed differences from controls  in
social  behaviors  (Schantz  and  Bowman,
1992). The mothers had been fed a diet
containing  5 ppt of TCDD.  The  offspring
tested for learning, from two cohorts, were
born  a mean of 16 months  or 36 months,
respectively, from the initiation of exposure.
Social behavior  was  assayed in  the  first
cohort. On the basis  of total TCDD con-
sumed bv the time of birth, the mothers had
       j
been exposed to about 0.125 ng/kg daily.

       Copulatory behavior in male rats and
measures  of morphological  and  endocrine
development showed adverse effects of prena-
tal exposure to TCDD at a dose of 64 ng/kg
to the mother on gestation day (GD) 15
(Mably et al., 1992).  If a half-life in rats of
24  days  was  assumed for  1  ng/kg  day'1,
steady state would be reached in about 120
days, and total body burden would be about
34 ng/kg. For this reason, because 64 ng/kg
is  actually  a frank effects level (PEL),  1
ng/kg/day would be a dubious NOAEL. The
problem  lies in distinguishing  between  the
acute effects of a  dose delivered  on GD 15
and an equivalent body burden stored largely
in fat tissue. TCDD stored in this way might
be viewed as functionally dormant, although'
the Schantz  and  Bowman data cited above
argue against such an interpretation. Com-
parisons  between  acute  and   steady-state
exposures for endpoints such as those above
have not been carried out  despite the signifi-
cance of this issue. Such  studies should be
relatively straightforward,  though tedious, to
conduct.

     In humans, TCDD and other lipophilic
agents could  enter the blood  if  fat stores
were to  be drawn upon  during  periods of
caloric deficit.  Weight-loss diet books  con-
sume enormous shelf space in bookstores and
weight-loss  regimens  are  not  a  guarantee
against pregnancy.  Although the increment
in blood level  of lipophilic toxicants during
such a regimen may be small, it may not be
insignificant, and fetal tissue might accumu-
late these increments.  Release of such agents
during gestation,  in the small proportion of
cases in which the mother fails to consume
adequate nutrition, might  lead to their accu-
mulation in fetal tissue. Moreover, the fetus
will deplete the mother of nutrients  even at
the cost of her own nutritional status.  Lacta-
tion is another situation in which  lipophilic
agents   are  released   and  consumed, as
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PAGE  58
discussed in the document.18  The lead paral-
lel  should  again be  considered.    During
pregnancy, lead is  released from bone as a
consequence of to hormonal changes. The
same  mechanism  would  not pertain  to
TCDD, but the  details of gestational phar-
macokinetics is  a question  that deserves to
be further pursued.

       Endpoints other than those discussed
above (sexual function in rats and learning in
monkeys) should also be considered. The role
that developmental toxicity has assumed in
risk assessment stems from an extensive body
of literature indicating the exquisite vulnera-
bility of the fetus. The developing brain is
especially sensitive.  But the rodent brain may
not  reflect  all  aspects  of this  sensitivity.
Recall that rats are born, from the standpoint
of brain development, at  the end  of the
second  human  trimester.    Processes that
occur in humans during the third  trimester,
such as synaptogenesis and the maturation of
neurotransmitter systems,  occur  postnatally
in rats. For this reason,  confining develop-
mental  treatments in rats  to the prenatal
period may  underestimate the full  impact in
humans of gestational exposure. The behav-
ioral  pharmacology literature  offers many
examples of the sensitivity of the neonatal
period in rodents and Bjerke  et al.  (1994)
and  Bjerke  and Peterson  (1994) reported
that lactational  as  well as prenatal exposure
proved necessary to induce  feminization of
male rat copulatory behavior.
          A study just published, and not available to
the Committee at the time of the review, reports that higher
levels of PCBs, PCDDs, and PCDFs in breast milk were
related to reduced neurological optimality, and higher levels
of pknar PCBs were associated with a higher incidence of
hypotonia (Huisman et al., 1995).
      Further, although  sexual development
has become  a focus of TCDD  toxicity re-
search, it is crucial to recognize that the com-
plex unfolding of brain  developmental  pro-
cesses in  the presence  of certain levels of
TCDD could also exert  an impact on other
indices of brain function and structure.  The
substantial levels of the Ah receptor in devel-
oping  brain  and  its  virtual disappearance
later in life argue that sexual development is
unlikely to be its only  role. The report by
Schantz and  Bowman (1989) hints at addi-
tional outcomes.  Note, further, that copula-
tory behavior and genital  structure reflect
only  limited  facets  of  sexual maturation.
Copulatory behavior and reproductive mor-
phology may not be the best endpoints to
examine   for  prenatally-exposed  females.
Other sexually dimorphic behaviors, based on
cognitive  function, for example, might  also
reveal consequences  of  TCDD  exposure if
examined. And, for males,  sexual motivation
is an  arena independent of copulation itself.

      The preceding comments presume  that
the total exposure is to  TCDD. They make
no  assumptions  about the  validity of TEQs,
which is a separate, but related issue.  Chap-
ter 5  of the  reassessment document neither
cited  nor discussed the findings  of  several
researchers on  the effects  of PCBs  (e.g.,
Jacobson and Jacobson, 1994 and Gladen, et
al, 1988).   Jacobson and Jacobson (1994)
reported  that higher gestational exposure to
PCBs, as reflected by cord serum levels and
maternal consumption of Lake Michigan  fish,
was correlated with lower  scores on tests of
psychological  development.  A large cohort in
North Carolina was reported  by Gladen et al.
to show also  evidence of poorer performance
associated with  higher prenatal exposure to
PCBs. Jacobson and Jacobson suggested that
the   PCBs   themselves,  particularly  the
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                                                                               PAGE  59
coplanar  PCB  congeners,  could be more
responsible for  such effects than the much
lower    concentrations    of   dioxins   or
dibenzofurans  in  these  mixtures, but  the
relative  potency  issue  would have   to  be
clarified.  PCDFs in the Yusho and YuCheng
exposures  are currently held to be primarily
responsible for the observed toxicities. The
North Carolina and  Lake Michigan studies,
however, are significant because they indicate
adverse neurobehavioral development associ-
ated with current environmental levels of this
class of compounds.19

       Another reason for  reevaluating  the
current NOAEL are data strongly suggesting
that TCDD is  a more  potent teratogen in
mice than  previously supposed, and that this
effect occurs at doses considerably lower than
those causing liver enzyme induction. (Bjerke
et  al.,  1994)  These  data are coupled with
findings  from  various  laboratories showing
changes  in hormone  levels controlling repro-
ductive  and  developmental  processes and
reproductive success in offspring  of treated
animals.

       In summary, the current NOAEL of 1
ng/kg day'1 rests on a debatable foundation, and it
would be appropriate  to reevaluate it.20   One
reason for a fuller examination  of these issues
is  that such a NOAEL is  likely to be used in
evaluating   the  risks  of  human  exposures,
given that crude clinical evidence for develop-
mental and reproductive effects attributable
to TCDD  is necessarily quite limited.  The
       19
          These findings were not discussed at the
Committee's public meeting, but came to light during the
preparation of this report.


          Several Members of the Committee suggest
that the immediate inference of this statement is that the
NOAEL should be lowered.
neurobehavioral studies on PCBs offer direc-
tions for additional research, but forcing such
studies into an effects level mold, as noted
earlier, is  a premature use of the data.

4.7   Human/Animal Databases: Poten-
      tial for Immunotoxicity   (Charge
      Question 15)

      There was a consensus among most Com-
mittee  Members that, overall,  Chapter 4 of the
reassessment  document  provided  an  accurate,
current summary of the immunotoxicology associ-
ated with   TCDD  and related compounds in
humans and  experimental animals.   However,
the  Committee   has  some  concerns  (and
noted  some  omissions) pertaining to immu-
nology and  the   interpretation  of  certain
experimental results,  in  Chapter  4,   and
particularly, Chapter 9. Although the overall
data suggest that dioxin  and related com-
pounds can produce  immune effects, there
are insufficient supporting data  to establish
fully whether these effects can  occur at or
near two  orders-of-magnitude  above back-
ground levels.

      The document hedges on whether there
was sufficient evidence to state that this class
of compounds can cause  immune effects in
humans.   In  some  instances there was  a
"YES" and in other sections a  statement of
"UNSURE."  Based upon  the extensive experi-
mental  animal, and the  very  limited  human,
database the  majority  of the Committee agreed
that sufficient data exist to indicate immune effects
could occur in the human population from exposure
to dioxin or dioxin-like agents at some dose levels.
However, the large variability in the immune
response in humans,  the  limited numbers of
tests  conducted,   and the  poor  exposure
characterization of the populations that have
been  studied prevent definitive conclusions
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PAGE  60
as to sensitivity.  This is not to say that
humans are more or less sensitive than other
species, only there are not sufficient clinical
data to assess human sensitivity.

       The  most notable  documented  im-
mune effects in humans occurred in the Tai-
wan population exposed to contaminated rice
oil  where both immunosuppression and  in-
creased infections were observed, presumably
resulting from exposure to furans and PCBs
(Wu et al, 1984; Chang et al, 1982a; Chang
et al.,  1982b: Chang et al.,  1981).  Some
studies    have   reported    changes    in
immunoglobin  (Ig)  levels  (Jennings et al.,
1988)  and  NK cell  activity  (Jennings et
fl/.,1988; Svenssonrtfl/., 1991). Other studies
have reported no effects (such.as the Ranch
Hand  (USAF,   1991)  and Seveso  studies
(Mocarelli  et  al,  1986;   Mocarelli et al.,
1991)). but, as with the positive studies, the
actual  exposure levels  and  study design, were
not adequately addressed in the document nor
were they critically reviewed.

       Although not well-established, several
studies of humans (Chang, et al., 1981; Bekesi
et a/,,1985)  exposed to halogenated aromatic
hydrocarbons (HAH), as well as of monkeys
(Hong et al, 1989; Tryphonas et al., 1989),
reported that a slight reduction in CD4"1" cells
occurred.   Although  this  may or may not
translate to a significant health effect, these
cells are involved in regulating immune  re-
sponses and reduced CD4/CD8 ratios are a
hallmark of immunosuppression.  It may be
argued that any reduction in CD4 cells could
lead to such potential  health effects as  in-
creases in infectious disease, given that a large
population is affected.  Of interest to this
discussion is a recent study by Oughton et al.
(1995)  which  found no  decrease  in  total
CD4+ cells in TCDD-treated mice following
low-level chronic  exposures. However, within
the CD4+  subset, a modest decrease  was
observed in the proportion of CD4+ memory
cells as denned  by concomitant expression
ofPgp-1 CD45RB. The clinical significance of
this change on immunocompetence  is pres-
ently  unclear.  Similar  changes in immune
system  functioning  have,  however,  been
suggested by some investigators to be signifi-
cant to  HIV pathogenesis  (Janossy et  al.,
1993; Lim et al.,  1993; Cameron et al., 1994;
Jaleco et al., 1994),  indicating  that  the  de-
crease in these cells may be associated with a
decrease in immunocompetence.

     Although the immune system is a sensi-
tive target  to HAHs in  experimental animal
species, as presented, the EPA document does
not provide convincing  evidence to indicate
that background or near background exposure
levels to dioxm-like compounds in industrial
countries are sufficient to affect the immune
system. Given the current methods available
for testing, it would be unlikely that this could
even be determined in humans, and one would
have to rely on experimental animal data or
highly   exposed  populations  to  determine
effects at the low-end of the dose-response
curve,  or in vitro approaches using primary
isolated  human  lymphocytes  and  human
lymphoid cell lines. Changes reported at very
low levels of exposure in two or three of the
experimental animal studies are certainly of
concern, but need to be confirmed and repro-
duced and, until then, cannot yet be used to
support a "background" level effect. However,
the No Observed Eeffects Level (NOEL) and
ED50 (dose effective for 50% of the recipients)
for suppression of the T-dependent antibody
response, in sensitive mouse strains, has been
reproduced in many labs   using  different
experimental designs and can be used to help
support or refute that "background" or one
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                                                                             PAGE   61
to two orders  above background is  signifi-
cant.  In this  respect,  the  recent paper  by
McGrath et a/. (1994) is relevant to this issue.

       Human studies  undertaken  to study
the immune system of exposed populations
have  not used the appropriate test battery
for this class of chemicals.  The "gold-stan-
dard"  test (i.e., suppression of the primary
antibody  response  following immunization)
was  not employed in any of the human test
panels, although this is  a hallmark in experi-
mental animals. The exception to this is the
as yet, unpublished study on the Inuit popu-
lation  (Dewailly, in press).

       Chapter Four discussions pertaining
to in  vitro effects,  although complete, con-
cluded these tests have limited relevance as
culture conditions may play  a significant role
(i.e.,  serum  effects).    The  Committee felt
that this was not a legitimate  argument as
numerous   investigators  have  successfully
reproduced  in  vivo  observations using  well-
defined in vitro culture conditions.  The in
vitro  studies   have  provided   considerable
understanding  of the cellular and molecular
mechanisms of TCDD and should not  be
understated.

       Although  data  exist  suggesting that
non-Ah receptor mechanisms may play some
role  in immunotoxicity, definitive  evidence
for this  is  lacking  and will require  using
novel approaches such as receptor knockout
mice   or  pure binding antagonists.    The
Committee  agreed that the majority of evi-
dence indicates that imununotoxicity (partic-
ularly  suppression of the antibody response)
by dioxins is presumably Ah-receptor depen-
dent.   The disappearance   of  quantitative
differences  in  immunosuppression between
Ah-low and Ah-high responsive mice  after
sub-chronic exposure suggests that chronicity
can  override acute exposure resistance and
may suggest an even "greater" hazard. Based
upon existing evidence, the involvement of
an endocrine-related non-Ah receptor mecha-
nism impacting on immunocompetence may
be overstated in the document.

     Numerous  studies  suggest that  the
immune system   is  a  sensitive  target for
dioxin-like  compounds in experimental ani-
mals.  There are species/strain differences in
the  sensitivity, but  the  effects  tend to be
similar with the most sensitive indicator (at
least in adult animals) being changes in the
primary  antibody  responses; similar effects
occur in many test species (guinea pig, rab-
bit,  monkey,  etc.).  As such, it would be
more appropriate to indicate that differences
in animal sensitivity exists rather than "vari-
ability in response" as  this suggests a differ-
ent meaning.  One might expect that similar
variability would  also exist  in  the human
population, but this has not been examined
when the limited  clinical  studies have been
undertaken.

     Multiple  cellular  targets   exist  for
immunotoxicity  by  dioxin-like  compounds
including both T and B lymphocytes as well
as lymphoid-associated tissue (e.g.,  thymus
epithelium)  and marrow  stromal elements.
Debate exists as to  the "most sensitive"  or
"most  proximate"  target and not which cell
is the  target.  The  reassessment document
should be more clear on this point. (Table 9-
2  is  incorrect as the  immune  system  of
rabbits and fish are also affected).

     Studies conducted in a number of exper-
imental species, including mice and monkeys,
indicate  that the antibody response to  a T-
dependent  antigen is the most sensitive and
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PAGE  62
reproducible indicator for immunotoxicity in
adult animals. The ED50 in sensitive strains of
mice is  approx.  0.7 Mg/kg.   Several other
responses have been shown  to be more sensi-
tive  but have not been  confirmed or repro-
duced by other research groups.  Chapter 9
gives undue weight to these  unconfirmed or limited
studies (see Table  9-5), and fails to discuss the
highly reproducible and widely  used primary anti-
body suppression studies.

       Results for the host resistance tests are,
for the most part, consistent  with the immune
affects. One obvious exception to this are the
influenza challenge studies conducted in mice
(G.  Burelson, in press), where  disease occurs
at much lower dose levels  than do  immune
changes.  As this directly relates to human
health, the mechanism and relevance of these
observations, which of course were not avail-
able when the reassessment was  developed,
need to be addressed in the future  revision.
The recent observations reported by the same
author in rats (G. Burleson, in press) should
also be  discussed.21  This  new study helps
increase the validity of the preceding observa-
tion, although additional  studies are war-
ranted to help elucidate the mechanism.  It
would argue that a very specific component
(perhaps immune/perhaps not)  is altered, and
at extremely low concentrations.

       Chapter 9 should  also include a table of
confirmed laboratory results (i.e.,  the PFC pri-
mary response) which provide EDSO, ED,, and/or
NOELs.    A separate  table for suggestive
results (not  yet extended at low  doses) can
then be  included  and  identified as such.
          The two in-press studies noted here were not
available to most Members of the Committee during the
review process; several Members therefore cannot endorse
these specific findings at this time.
The text of these tables  should include a
critical  review  of the data, the most repro-
ducible  and sensitive indicators, and a clear
and logical presentation of how these data
were used  to determine that exposures at 1
or 2 orders-of-magnitude above background
levels have potential human health effects.

4.8   Other Effects  (Charge Question
      16)

      The  Committee had no specific con-
cerns with  the  manner in which the topic of
"other effects" is covered in the reassessment
document.    There  has  been  considerable
expansion  of the knowledge base  since the
document  was issued,  however,  and these
gains should be addressed in any revision.

      Specifically,  major growth  has taken
place in our understanding of the biological
and biochemical effects of TCDD and  re-
lated members of this class, and in the whole
area of receptor biology and  signaling biol-
ogy, and these gains should be factored into
the revision.

4.9  Dose-Response

4.9.1   Approaches to Dose-response
        Determination for Cancer
        (Charge Question 17)

      There was public expectation that the
reevaluation of the  carcinogenic potency of
dioxin would  be comprehensive and would
incorporate extensive new data generated for
that purpose.  The  Committee was disap-
pointed to  see that, in addition to data from
the  Ko'ciba et al.  (1978)  bioassay (which
formed the basis for EPA's  earlier estimate of
cancer  potency for TCDD), the  only addi-
tional data used in EPA's quantitative analy-
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sis  was  from the  Maronpot et al.  (1993)
gavage study.
       The only  use  that EPA  made  of
pharmacokihetic modeling in its quantitative
analysis was in  making correlations between
estimates of two-stage model parameters and
outputs of pharmacokinetic models (Appen-
dix D of the reassessment document).  This
is a very limited use of the  physiological and
pharmacokinetic information  (although the
Committee is aware of the problems involved
in using  the  extant  PBPK models-see the
discussion in sections 4.1.1 and 4.1.2); more-
over, the description provided in Appendix D
is  not  sufficient  to  enable the  reader  to
understand what  was  done.   This  lack  of
clear exposition needs to  be corrected in the
revised document.

       Not only is EPA's risk assessment  of
dioxin extremely important in its  own right,
it  represents  EPA's  first  application of so-
called  "biologically based"  models.  Conse-
quently, it may set  a precedent for future
analyses.  It is  thus important that the analy-
sis be  clearly  presented .so  that its scientific
justification and usefulness can be assessed.
EPA should describe its analysis in sufficient detail
that it can be fully understood by the reader, to the
point of reproducing the analysis if desirable.  The
reasoning and analysis  that led EPA to  pro-
pose  its  preferred model  must  be clearly
explained.  The sensitivity of the  results  to
alternative models or assumptions must also
be presented.  Unfortunately, EPA's descrip-
tion of its analysis  is  lacking in clarity,  in
details, and in supporting documentation.

       The description of the analysis relied
upon  by EPA consists of a single paragraph
at the bottom of  page 8-45.   The  resulting
               .                 PAGE  63

 model  is described in broad terms  as  the
 "most  parsimonious two-stage model" that
 agrees  with the tumor incidence data and
 focal lesion data.  EPA must describe its analy-
 sis in sufficient detail to allow the reader to under-
 stand how EPA arrived at its preferred model and
 how robust those results are - i.e., to what extent
 would other assumptions be reasonable, adequately
fit the data, and lead to different levels  of risk.
 For example, EPA's preferred analysis uses
 data from a feeding study and a gavage study
 and thus had to make  assumptions in order
 to combine data from two  different types of
 studies.  EPA does not describe how this
 issue was handled,  nor detail the necessary
 assumptions.

      Also,  EPA  does  not  describe  clearly
 what data were used in its preferred analysis.
 It states only that the  tumor incidence data
 from the Kociba et al. (1978) study and the
 focal lesion data from Maronpot et al. (1993)
 were used.   The  precise  form of the tumor
 data (whether  individual  animal  time-to-
 tumor data or summary data) is not stated.
 Similarly, the  reader is not  informed as  to
 whether  the data  from  Maronpot.   et al.
 involving initiation with DEN or the  data
 not involving such initiation were used  in
 EPA's preferred analysis.

      The focal lesion data used in develop-
 ing EPA's cancer risk model are not from the
 published Maronpot et al. (1993) paper but
 are  unpublished  results  from that  study.
 That fact should be clearly indicated.  More
 importantly, the  actual  data used by EPA
 should  be made accessible to the reader, by
 including them in an appendix, if necessary.

      If time-to-tumor data  were used, an
 assumption was required as to the context in
 which  the  hepatocellular tumors  from the
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PAGE  64
Kociba et al.  (1978;  1979)  studies  were
observed  (whether incidental,  fatal or  some
combination).  This assumption is not stated,
and it may have an important  effect  upon
EPA's conclusions regarding the fits to the
data of various models.

       Appendix C contains additional details
of some analyses, but not  the ones  used  to
derive EPA's preferred cancer model. A con-
siderable portion  of Appendix C is devoted to
discussing the non-identifiability  of  some
parameters in certain applications of the two-
stage model.  In some of these instances, EPA
is attempting to estimate up to  16 parameters
from what are essentially four data points.  It
is not surprising that some of the parameters
are not identifiable.  The  last paragraph  in
Appendix C is  particularly disturbing.   It
starts with the assertion that the  calculations
have "hidden assumptions" that can have a
bearing on the interpretation of the results.  It
then goes on to list some  of  these "hidden
assumptions"  and ends with the statement
that the analysis is very sensitive to the choice
of values  for the radius of  a cell and to the
(assumed) minimum size  of a detectable
focus.  This paragraph (and particularly the
last statement re sensitivities of the analysis)
raises serious questions  as to the  reliability of
EPA's cancer risk assessment. No  information
is  provided  on the  cell radius or minimum
detectable focus size assumed in EPA's analy-
sis.  In fact,  this is  the  only mention that
either  of these two quantities are required at
all  in the analysis.  EPA must provide enough
detail in its analysis to permit the reader to deter-
mine how  these values  were used  in  the analysis,
how EPA  selected  those values, what values were
selected, and the sensitivity of EPA's risk assessment
to those selections.
      EPA must clearly distinguish between
what is being assumed (i.e., what is going into
the modeling) and what is being concluded as
a  result of the modeling.  Although EPA's
preferred dose response model is linear, it seems clear
that a threshold model would provide an equivalent
or nearly equivalent description of the data.  This is
the most important issue in the dose response-model-
ing and should be thoroughly explored in EPA's
analysis.

      Even if EPA's risk assessment based on
the animal data is  correct, without additional
assumptions regarding the relative sensitivities
to dioxin of various  types of tumors  in hu-
mans and animals, the  risk assessment based
upon animal data  only provides estimates of
the risk of liver tumors in a single strain of
female rats. Therefore, despite limitations in
the human data for dioxin, it would have been
appropriate for EPA to have conducted a more
comprehensive analysis of the human data.
EPA's risk assessment based on human data is
derived from  the  published data  from three
studies.   Reliance on  these published  data
necessitated a number of assumptions and
approximations by EPA that could have been
avoided by use of the raw data  from other
studies.   The Committee also recommends
that the data from the Ranch Hand cohort,
when published, be considered for inclusion in
this analysis.

      The cancer  risk assessment  models
applied to the human data by EPA are con-
ceptually flawed.22   Both the  additive and
multiplicative  models  express  the  cancer
mortality rate at a  given age as a function of a
summary measure of exposure.  Clearly, this
                                                         Several Members of the Committee contend
                                               that the cancer risk model is not particularly "flawed," but
                                               represents an acceptable approach.
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                                                                             PAGE  65
summary measure should involve only prior
exposures and  not future exposures.  How-
ever, the summary exposure measure used by
EPA is average lifetime  exposure, which in-
volves both  past  and future exposures.  A
practical   consequence   of  this    model
misspecification is that  in  the case of the
additive  model, the  increased mortality rate
under constant exposure is independent of age
(e.g., a person has  the  same probability of
dying of a dioxin-induced cancer between the
ages of one and two as between the ages of 70
and 71), which is clearly inappropriate. EPA
appeared to recognize this and made a com-
pensating ad hoc adjustment to the risk esti-
mate obtained from the  additive risk model.
The Committee recommends that both the
additive  and multiplicative  models be refor-
mulated   to  incorporate more  biologically
plausible  summary exposure measures (e.g.,
cumulative past exposure).

       In EPA's cancer risk calculations based
upon  epidemiological  data,  exposures  in
dioxin-exposed subcohorts were summarized
by  the median exposure. The stated reason
for choosing the median (over, say, the mean)
was that body levels were "quite variable and
not symmetrically distributed."  Neither of
these assertions appear to be  supported by the
draft document.  At any rate, these are not
appropriate reasons  for selecting the median
over the  mean as  a  summary  measure of
exposure, and  there appears to be no clear
reason stated for preferring  the median over
the mean.  This choice would not be required
if the  raw data were used  in the analysis,
which illustrates an advantage of basing an
analysis upon the raw data.

       Given the problems  and limitations
identified with the analysis, it is not clear that
this work added significantly to our under-
 standing  of the dose-response for TCDD.
 Rather than giving a high priority to refining
 these  techniques at the present time, the
 Committee recommends that EPA review this
 effort, and communicate clearly the strengths
 and limitations  of  the  work.   The Agency
 should evaluate  critically  the potential  for
 future work in this area to elucidate the dose-
 response for TCDD in humans.

 4.9.2   Use of the RfD in Evaluating In-
        cremental Exposures (Charge
        Question 18)

      The question of possibly rejecting of the
 Reference  Dose (threshold)  approach  for
 evaluating  incremental exposure because of
 the existing background levels relates also to
 health Charge questions 14 and 17 (as noted
 earlier).  All three issues are part of a  more
 fundamental  problem which has not  been
 addressed and needs  to be included to provide
 balance to the reassessment document. This
fundamental issue concerns the basis for the selection
 of the  dose  response  relationship to  be used in
 assessing the (non-cancer) adverse effects of dioxin.
 The selection of the dose  response  function
 clearly distinguishes the EPA approach from
 that of some (but not all) other agencies and
 bodies which have  carried out similar risk
 assessments on dioxin.  Although all of these
 groups used the same toxicologic and epidemi-
 ologic  data bases as EPA, all except EPA have
 elected to  use some type  of threshold and
 safety factor methodology for their health risk
 evaluation.  Chapter 8 of the reassessment docu-
 ment needs to describe and evaluate this alternative
 dose response relationship,  discuss the approaches
 and findings of the other relevant agencies, and
 justify the basis for selecting another approach.

      Although the Agency concludes (page 8-
 13 of the reassessment document)  that the
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PAGE  66
use of the linear multistage  model  (LMS)
needs to be re-evaluated, the re-evaluation
consists of  enhancing the LMS approach
with a PBPK analysis and a 2-stage analysis
rather than a  discussion of alterative  ap-
proaches.  A discussion  of such alternative
approaches, and their results,  should also be
reflected in the appropriate  places  in  the
summary chapter 9.   As noted earlier in  this
report (see sections  4.5.2 and 4.5.3), dioxin
is  not an initiator and thus  is not a com-
plete  carcinogen.  Dioxin is a non-genotoxic

 ...  the available information on
  TCDDs suggest that use of the
   benchmark approach, rather
    than the reference dose, is
   probably more appropriate.
promotor which acts at least  in part via the
Ah receptor  and  exhibits  numerous  U-
shaped dose responses (Kociba  et al.,  1978;
Pitot  et al,  1987;  Maronpot et al,  1993;
Teegaurden  et  al,   1995; Fang et  al,  in
press).  Thus, the document cannot ignore
a  possible threshold dose-response relation-
ship and claim to be  comprehensive in its
presentation.

      As noted in the Committee's response
to health question  14 (section 4.6.1),  the
available information  on  TCDDs   suggest
that use of the benchmark approach, rather
than  the reference  dose, is probably more
appropriate.  This  approach has  been  recom-
mended  in  several  previous SAB  reports
(SAB, 1990; SAB,  1995).  The EPA, along
with the International Life Sciences Institute
(ILSI),  has  sponsored workshops on  this
topic, and various EPA staff  are among the
most  progressive and knowledgeable  experts
in the use of this methodology.
     The Committee (with the exception of
one  Member) agrees  that, in concept, the
reference dose is not designed to evaluate the
risk from incremental exposures (however,  if
background exposures are not accounted for in
the population from which data are obtained
for calculating a reference dose, the resulting
reference dose may represent doses in excess
of that background). Although EPA's current
methodology for cancer risk assessment allows
one  to  assess risks from incremental expo-
sures, the RfD methodology is not well-suited
for this particular use.  The Committee recom-
mends that EPA work towards  developing and
implementing a methodology that would allow the
assessment of non-cancer risk resulting from  incre-
mental exposures.

4.9.3  Continuum of Response Postulate
       (Charge Question 19)

     EPA postulates a continuum of response
from events seen at low doses that are not
toxic but cause  the subsequent development
of toxic effects.  This idea is expressed several
times in the document, but it is not supported
by a full discussion.   As it stands now, the
basis for this statement regarding a continuum
of responses is unclear.  The statement is far
too general and could be taken as implying
that all (or any) early changes will necessarily
lead to ultimate toxicity.  The statement  is
only defensible in reference to a limited num-
ber of specific case examples, but cannot be
taken  as  universally  proven.   Until a full
mechanism of action  has been mapped out,
the reassessment's position remains unproved
in general.   The  statement should  not be
presented as  a  "postulate"  (which is widely
accepted as a universal truth not requiring
proof)  but as a  "current hypothesis" (subject
to change as new data are discovered).
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       The specific case developed  by EPA to
support this  hypothesis was the binding of
ligand to the Ah receptor, which is then as-
sumed  to  lead to all, or most,  of the toxic
responses.     The  possibility  that  the  Ah
receptor system may be a sensing  pathway
to protect the cell, not an integral part of
the  machinery associated  with  the  toxic
response of a cell to TCDD, is not consid-
ered.23   Only  in the mechanism  of action
chapter  is there any  suggestion  that this
association may not be universally accepted.
However,  Ah receptor binding  may not be
the  ultimate  mechanistic  step in  all  re-
sponses.   It is  not proven for all possible
toxic endpoints; the  association is strongest
for enzyme induction in mice, but may not
hold in other  species.   Different strains  of
rat show  remarkably diverse sensitivities  to
dioxin while  possessing  active Ah receptors.
Although  enzyme induction in mice is  the
classic Ah  receptor  mediated response, there
may be other responses that do not involve
Ah receptor.   For  example,  although some
immuno-responses are Ah associated in mice,
others  are apparently  not.24    Ah  receptor
binding may not be a rate-limiting  response.
The  recent  development  of  "knock out"
mice lacking  functional Ah receptors may
help  clarify  these  points.   EPA  needs  to
leave itself some flexibility so  that the  as-
           One Member of the Committee asserts that
"The Ah receptor system is probably part of the normal
cellular second messenger regulating systems and its
inappropriate activation by dioxin (and dioxin-like
compounds) can lead to assorted toxic outcomes through a
variety of pathways.  Any suggestion that this inappropriate
activation has positive outcomes is strictly speculative at this
time."

           One of the Committee's immunologist
participants believes that "The evidence for receptor
independence from this data is insufficient to contra-
dict the majority of evidence for receptor dependence."
sessment can remain valid in  light of future
discoveries.

      The EPA response at the public review
meeting suggested that Ah receptor binding
could  be considered as "necessary  but not
                                  y
sufficient."  Other events may well  also be
needed for toxicity to occur.  Because of this,
TEFs should be based solely on Ah  receptor
data only when other data are unavailable.
There  are   a  variety of  individual effects
yielding a likelihood of response, a cascade of
events.

      The  Committee is not taking the position
that EPA is  wrong in  its  view that Ah receptor
binding is a critical early event leading to eventual
toxicity, but rather that the Agency stated the idea
too strongly and without sufficient consideration of
toxic immune system effects  that  have  not been
shown  to be Ah  receptor associated.   Alternative
mechanisms that have been suggested in the pub-
lished literature were not considered  in the docu-
ment.   The evidence for Ah receptor-  linked  effects
(and their role in toxicity), and (for balance) the
evidence suggesting a lack  of involvement of Ah
receptor binding in some effects, should be discussed
in the document  to  support acceptance of this
continuum as a current hypothesis.   Furthermore,
the continuum theory needs further explana-
tion,   some  discussion  of the  merits  and
limitations,  and an indication of the accep-
tance  of this idea in the  scientific  commu-
nity.   EPA  needs to be more flexible in its
statements, to allow adaptability to scientific
evidence that  may be developed  in  the  fu-
ture.

4.10  Use of Toxicity Equivalence  Fac-
      tors (TEFs) (Charge Question 20)

      In general,  the  Committee agrees that the
use of a TEF is a valid approach provided that the
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contribution to the TEQfrom: a) TCDD; b) other
dioxins and furans; and c)  coplanar PCBs are
explicitly  stated.   However,  when  assessing the
toxidty of complex mixtures that are not well-defined,
the Committee believes that presenting the results
using alternative methods may be warranted when-
ever possible.  It must be noted however, that
other than the suggestion (see  Section  4.13
below) to apply TEQs separately for 2,3,7,8-
TCDD,  other  dioxins and  furans, and co-
planar PCBs,  as well as  for environmental
mixtures as a whole, the Committee has no
specific proposals  for  such alternative meth-
ods.

       Although the assessment acknowledges
some of the uncertainties associated with the
use of TEFs/TEQs, these issues have not been
satisfactorily addressed.  Since  the  TEQ ap-
proach has  been used throughout  the assessment
document, and many of its conclusions (e.g.,, the
position that levels 10-100 times over background
pose a possible human health  hazard) hinge on the
validity of the TEF values and assumptions used,
the Committee advises EPA  to include a peer-re-
viewed appendix that will comprehensively  review
EPA's use of the TEF/TEQ approach in the exposure
and health assessment documents. This appendix
should clearly outline the assumptions and
TEF values used throughout the documents as
well as address the following issues:

       The reassessment document acknowl-
edges that dioxin-like  compounds other than
TCDD  represent  greater  than 90%  of the
calculated  TEQ value  in  some  instances.
Consequently,  the applicability of using TEFs
for mixtures  containing PCBs with  partial
agonist and antagonist activities should be
addressed.   The EPA assumes  that there  is
additivity among  TEQs calculated  from the
TEF values for 7 of the  75 dioxins,  10 of the
135 dibenzofurans, and 13 of the 209 PCBs.
However, these dioxin-like  congeners consti-
tute a small percentage of the total congeners
present in an environmentally relevant mix-
ture. Therefore, the EPA should address the
issue of possible interactions, since  there  is
evidence that non-dioxin-like PCBs antagonize
several  biochemical (e.g. enzyme induction)
and toxic responses  (e.g.,  teratogenic  and
immunotoxic effects) elicited by more potent
congeners.  Possible synergies should also be
considered.

       New data, which became available since the
release of the document have resulted in adjustments
to several of the TEF values.  The Committee sug-
gests that a comprehensive review of all TEF values
be summarized within the appendix for each congener
that has previously  been assigned a  value.   In
addition,  EPA should clearly state the species
and responses  (e.g., ligand binding,  enzyme
induction, imrnunotoxicity)  used to derive the
TEF value.  Finally, since TEF values  can vary
dramatically based upon the species and re-
sponse examined, EPA should justify  the TEF
value that has been selected for evaluating
human risk.

       EPA  should  document  clearly  the
studies  that demonstrate  additivity among
dioxins, dibenzofurans and PCBs and that the
TEF/TEQ approach accurately predicts both
short-term (e.g., immunotoxic effects, enzyme
induction) and long-term (e.g., carcinogenic,
teratogenic,  reproductive  effects)  responses
elicited by these complex  mixtures.   In the
event there are insufficient data demonstrating
the applicability of the approach for specific
toxic endpoints,  EPA  should justify  its posi-
tion for the use of the TEF/TEQ approach.

       Although the reassessment recognized
that humans are not overly sensitive or resis-
tant to the effects  of dioxin-like compounds
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                                                                            PAGE   69
relative to other species,  there is a lack of
discussion regarding the differences between
human and rodent receptors.  Such informa-
tion should be included in the appendix.  EPA
should also outline how this  information is
taken into account when assigning TEF values
to congeners.  Also, recent studies indicate
that there are differences in congener uptake,
metabolism,  elimination, and storage.   This
should be acknowledged followed by a discus-
sion of how this information is  taken into
account when assigning TEF values to conge-
ners.

       The   reassessment  document  lacks
discussion   regarding  naturally   occurring
dioxin-like     compounds     such      as
benzo[a]pyrene  and indole[3,2-b]carbazole.
The EPA should comment on the exclusion of
these  compounds.   These  agents  are  not
persistent or bioaccumulative, but the precur-
sor of indole[3,2-b]carbazole is  present at
high levels in the diet, and this constant level
of exposure should be reported. Furthermore,
there is (very preliminary) evidence that some
of      these      compounds      (e-g->
indole[3,2-b]carbazole) may have an anticar-
cinogenic effect  (Bjeldanes  et   al.,  1991;
Bradlow  et al.,  1991; Liu et al,  1994; and
Wattenberg et al., 1978).

       As mentioned in the health assessment
document (p 9-70),  "A more detailed descrip-
tion of these issues  is contained in U.S. EPA
(1989)."  This referenced document (TITLE,
EPA/625/3-89/016), once updated and modi-
fied to address the issues listed  above, and
peer reviewed, could be used  as a basis for the
appendix  and  therefore,  could  reasonably
accommodate the Committee's above sugges-
tions.  In addition, a balanced comprehensive
review should clearly state  the assumptions
and limitations  of the TEF/TEQ approach as
well as highlight the areas that warrant further
investigation.  Examination of the Summary
of the Public  Comments  related  to   the
Exposure     and     Health     documents
(EPA/600/6-88/005Ca,    Cb,    Cc    and
EPA/600/BP-92/001a, 00Ib, 00Ic) indicates
that EPA is  already  aware of the concern
surrounding this issue  (i.e. the statement that
"This issue  was the  single  most addressed
issue among all of the comments  on  the risk
characterization"  on page 25 of  the Health
Comments Summary). Therefore, the addi-
tion of a peer reviewed appendix dealing with
the TEF/TEQ approach should satisfy several
of the concerns raised, or at least indicate
which items are still points of contention.

4.11  Laboratory Animals/ Human Re-
      sponse

4.11.1   Animal Data and Weight-of-
         Evidence Conclusions for Hu-
         man Risk (Charge Question
         21)

      The present state of our knowledge of
TCDD as a toxic agent is largely derived from
studies with animals. Thus, there is, by defini-
tion, a heavy reliance on the results of numer-
ous animal  studies designed to  define the
plethora of effects of TCDD. By default one is
compelled to use these animal data.   However,
these  data  must  be  used  in perspective.
Studies  using  animals as  surrogates  for hu-
mans  show  almost a  10,000-fold range in
their response to dioxin exposure  for a  num-
ber of different adverse effects, depending on
the animal species  and endpoints selected.
The reasons  for  these  large differences in
response by different  species of animals  are
not known (even though they are all pre-
sumed to have the Ah  receptor), but this
breadth of sensitivity may serve as an indica-
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PAGE   70
tor of the range of response that might be
seen in the human population.

      The  use of animal data to establish a
potency value for TCDD which may then be
used for calculating human risk is of particu-
lar concern to this Committee. The assign-
ment of "oral intake risk-specific doses of
slightly  less  than  0.01  pg TCDD/kg/day,
corresponding to unit risk estimates of 1 x
1Q-4 per pg TCDD/kg/day"  (pages 9-67 and
9-83) must be rigorously analyzed to satisfy
the scientific community that  these values,
derived solely from animal  studies, are ade-
quately justified  and  defensible.    These
critical values will serve as the elements  for
human risk assessment  calculations by risk
managers when determining acceptable levels
of human exposure.  It is not obvious how
these values  of potency were derived and
how vigorously the value can be defended as
the  critical  number used  for human risk
calculations.  The  document requires  a more
extensive discussion  (in Chapter 9)  of how this
calculation was derived, which default assumptions
were used,  and why daily dose  is  the  proper
dosimetric parameter.

4.11.2 Animal vs. Human Data
        (Charge Question 22)

      The  strengths and weaknesses  of ex-
trapolation  to humans  are  discussed in the
dioxin  document,  in the responses by  those
submitting  comments,  and in the  TCDD
literature.   In general terms, the arguments
advanced from both  positions  are widely
recognized.   Little  is novel in the TCDD
context and  the  debate: remains relatively
superficial.  EPA's assertions in favor of spe-
cies  extrapolation   are  based  on  the
weight-of-evidence argument, which points to
some shared mechanisms among species, the
presence of a functional Ah receptor in hu-
mans, similar biochemical responses in multi-
ple species, and the correspondence in certain
outcomes  of high dose exposures, primarily
chloracne,  in  both  humans and  animals.
Commonalities  in  certain outcomes among
several species of laboratory animals (but not
yet humans),  particularly  in  the  growing
developmental literature, also  support this
position.

      The opposing  position  asserts that
postulating  homologous  outcomes  among
species is not a fruitful avenue to risk assess-
ment. This position objects particularly to the
lack of cogent human data on indices such as
sexual  behavior, immune dysfunction, and
others, and notes species heterogeneity in the
Ah receptor.  Lack of human data, however,
should not be interpreted to mean that hu-
mans  are unaffected,  because  appropriate
human  studies  are  yet  to be  performed.
Although it will be a difficult task, EPA should
perhaps consider, in future revisions,  what
human studies might be undertaken to shed
light on this issue.

   ... a revised Chapter 9 should
   feature developmental toxicity
    with as much emphasis as
     is allocated to cancer and
	reproductive toxicity.	

      The animal data indicate TCDD to
be a potent developmental toxicant capable
of inducing functional  and morphological
aberrations in male rats (Mably et «/., 1992;
Bjerke et  a!.,  1994;  Bjerke and Peterson,
1994).  Because such outcomes can occur at
low  doses, and therefore  are much  more
relevant  to general  environmental  levels,
most  Committee  Members think  that a
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                                                                              PAGE   71
revised Chapter 9 should  feature  develop-
mental toxitity with as much emphasis as is
allocated  to cancer and reproductive toxic-
ity. Animal  data  will have  to be  relied on
for  developmental  questions  linked  to
TCDD because the  human  database  is so
limited. These data, however, can be supple-
mented by  the  PCB data  from the  Lake
Michigan  (Jacobson and Jacobson, 1994) (as
discussed     in  section  4.6.1)   and North
Carolina  (Gladen et  al,  1988)  studies be-
cause, unlike the Japanese  and Taiwanese
episodes,  they are based on prevailing  envi-
ronmental  levels  and  were revealed  by
neuropsychological   indices   rather   than
clinical abnormalities. The  evolution of our
understanding of the developmental toxicity
of lead should be cited as a model.

      A  lamentable stimulus  to this  debate is
the somewhat loose and  impressionistic manner in
which Chapter 9 is framed.  Had its tenets been
offered in a more precise, organized manner,  with
appropriate documentation and depth,  and  with
a clear presentation and evaluation  of conflicting
points of view, it could have served as a structure
on which to  build a more defensible risk charac-
terization.   Although  the case for extrapola-
tion is argued more  cogently in  Chapter 8,
its  fragmentation  in Chapter 9  leads to
questions  about its validity.  In defense of
EPA,  however, the  insistence  on  human
evidence for every assertion  about a possible
hazard  based on animal data is  unrealistic.
One comment submitted to EPA contends
that clinical evidence of disordered human
sexual  behavior,  corresponding to  Mably et
al.  (1992),  has yet to  appear (however,
complaints of impotence have been received
from exposed workers, although adults  seem
to be a rather less sensitive  population). It
further  argues  that  the  preponderance of
social  and  environmental  determinants in
human sexual behavior  makes  it  unlikely
that  effects  homologous  to Mably  et  al.
would ever  emerge  as  a  consequence  of
prenatal TCDD  exposure.   No cogent  at-
tempt to make such a comparison has been
carried out;  in addition,  it would be naive
to expect  exactly the  same  outcomes.   If
such  studies  are  undertaken seriously, they
should  be   designed  to  illuminate  small
functional differences; the lead, ethanol, and
PCB  literature offer suitable models  (e.g.,
Streissguth et al, 1990 re ethanol).

       Chapter  9  needs  to  offer  a  balanced
perspective on  these issues, especially by recogniz-
ing that, for developmental toxicity, arguing from
simple homologous responses is unproductive.  An
appropriate translation, especially  for func-
tional endpoints,  is essential.   Chapter  9
should  be  organized in  accordance  with
Wilson's  principle,   i.e.,   that   functional
effects of gestational exposure are likely to
emerge  at  lower dose   levels  than  those
provoking overt structural teratologies (Wil-
son,  1977).  Chapter 5 might  also benefit
by a  wider  discussion  of developmental
questions beyond the  narrow  domain  of
TCDD.

4.12   Overall Scientific Foundations of
       the Health Reassessment Docu-
       ment

4.12.1  Evaluation of the Risk As
         sessment Chapter (Charge
         Question 23)

       The stated purpose of the Risk Char-
acterization  Chapter (9)  is to  provide  "a
balanced picture of the scientific findings of
the health and exposure assessments for use
by risk managers  in selecting risk manage-
ment options ..."  (pg.  9-2).   The risk
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PAGE   72
management implications of the draft conclu-
sions could certainly be  significant.  If there
are  public  health consequences  of current
exposures to dioxin-like  compounds, intensi-
fied regulatory actions may be appropriate.
Given the large public health and economic
stakes in the dioxin reassessment, the Agency
is well-advised to make sure  that its  final
conclusions  about  dioxin-like  compounds
have  a high degree  of support within the
scientific community.  Otherwise, risk manag-
ers will not be in a strong position to perform
their roles with competence and credibility.

       Overall, the analytic strengths of the draft
conclusions rest in the innovative approach that the
Agency has taken to deal with  this class of com-
pounds. The weaknesses rest in the lack of discus-
sion  of alternative low-dose  models  and serious
uncertainties,  and the  absence of quantitative
information that can be useful to risk managers in
comparing the incremental benefits  of regulatory
alternatives aimed at reducing exposures to dioxin-
like compounds.

       Looking at the strengths of the conclu-
sions, three major points are  apparent.  First,
by focusing serious attention  on various non-
cancer effects,  the Agency  has dispelled any
mis-impression  that EPA's  risk  assessment
process is overly preoccupied with carcinogenic
effects. In order to emphasize this point,
the  Agency  may  want  to  reorder  the
discussion of  health effects on  pp.  9-39
through  9-53, so that the discussions of
non-cancer effects  precede  that of the
cancer effects.

       Second,  by evaluating an  entire group of
compound classes (with  a com- mon attribute),
rather than a single compound,  the  Agency re-
sponds to the generally-mistaken criticism  that its
risk assessment process can only address issues on
a chemical-by-chemical basis.  In this case, the
compound  classes are  defined in terms of
common  biological responses, since  the hu-
man body is presumed to be responding to
the cumulative exposure to numerous agents
that  act  through  a  common  mechanism
involving the Ah  receptor.  This  leads to the
Agency's use of TEFs as  a numerical device to
describe the relative importance of various
dioxin-like  compounds.  In the  final assess-
ment,  EPA should  emphasize  further the
ambitious and somewhat speculative nature
of  this  ground-breaking approach  to  risk
assessment,  as well  as  the consequences of
not using a TEQ. approach.

       Third, in the opinion  of most Committee
Members,  a  useful  comparative  perspective  is
provided in the draft conclusions  where the Agency
highlights  the fact  that the margin of safety (be-
tween background exposures and  levels of exposure
where effects have been observed in test animals) for
dioxin-like compounds  is  smaller than the EPA
usually sees for many other compounds.

       Three major  weaknesses  were  also
noted. First, the presentation of scientific findings
portrayed in the draft conclusions is not balanced.25
A tendency  to   overstate  the evidence of
danger is  apparent in the following:

a)     There is an inference that humans are at
       risk from background and  near- background
       exposures.  The term "background," because
       of  its implications in  ordinary discourse,
       needs to be  amplified in the context of the
       dioxin reassessment.   "Background" typi-
           Several Members of the Committee do not
agree with this statement and regard the EPA presentation
and the inferences (drawn as appropriately conservative
within the context of public health protection.
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                                                                                  PAGE   73
cally  refers to exposure levels that are not out of
the  ordinary  experience.    The  populations de-
scribed  by Jacobson  and  Jacobson  (1994) (as
discussed in  section 4.6.1), Gladen  et al.
(1988),  and Huisman  et al.  (1995),  which
demonstrate 'associations  between PCB  (and in
the Huisman study, PBBs and dioxins) exposure
and neuro-developmental deficits, would be classi-
fied at the high end of the "background" distribu-
tion.   This  distinction needs  to made  clear by
EPA.

b)     The  Agency's decision to propose  a
       uniform  carcinogen  classification for
       all   dioxin-like    compounds   (even
       though the weight-of-the-evidence on
       TCDD  is more  persuasive than for
       many other  compounds   included in
       the report).

c)     The Health  Reassessment document's
       presentation of quantitative estimates
       of carcinogenic  potency without pre-
       senting  any quantitative  estimates of
       anti-carcinogenic action (even though
       available data suggest protective ef-
       fects  are occurring at low levels of
       exposure) and biological  plausibility
       for such effects (As  noted in section
       4.2.1  however,   several Members of
       the  Committee  suggest that possible
       protective effects may be related to
       anti-estrogenic activity,  rather  than
       any  general  chemoprotective  effect.
       One  Member does not  believe that
       the  evidence  for protective effects is
       statistically significant.).

       Second, important uncertainties associated
with  the Agency's conclusions are not fully recog-
nized and subjected to feasible analyses.  Examples
of this problem include  instances in which:
a)     The  document  did not acknowledge
       the biologic plausibility of a threshold
       model for some or all of the effects.

b)     Sensitivity analyses of the TEF values
       reported in Table 9.1 were not pro-
       vided (incorporation of such analyses
       were  suggested and  illustrated in the
       comments provided by the public).

c)     The document repeatedly referred to
       "average body burden" as a biologically
       meaningful  dose metric, even  though
       other measures of dose  associated with
       peak  intake may be more important
       for some effects, and that "area under
       the curve" is  the  preferred dosimetric
       for dealing with agents with long bio-
       logic half-lives.

d)     The document reported "conservative"
       numerical values (e.g.,  for the potency
       figure) without any realistic or central
       estimates to provide a broader perspec-
       tive.

       Finally, the characterization  of non-cancer
risk  is not performed  in a manner that allows
meaningful analysis of the  incremental benefits of
risk management alternatives.26  The  risk man-
ager is provided with no quantitative indica-
tion of how the severity or frequency of non-
cancer effects (e.g., reproductive and develop-
mental toxicity) in  the human population
might be affected by incremental reductions
in either intake rates or average body bur-
       26
          A minority within the Committee finds the
non-cancer risk characterization to be appropriate for use
within a public health perspective. However, they agree that
the reassessment document's characterization is not per-
formed in a manner which will be very useful in the analysis
of the incremental benefits of risk management alternatives
by those who are also concerned with the micro-level
economic costs.
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PAGE  74
dens.   Although  the  Agency  is  correct in
pointing out the weaknesses in a  traditional
"reference  dose"  approach  to  this  class of
compounds, it has not provided risk manag-
ers  with  any  means  to  perform  dose-re-
sponse  analyses of  the  non-cancer effects.
Unless such information is  provided, there is
a danger that the  real  possibility  of  non-
cancer effects will  be downplayed  or ignored
or,   conversely,  overstated,  in   regulatory
impact  analyses  and   future  cost-benefit
analyses.

       From  a  presentational  perspective,
more thought needs to be given to  how the
information  is  presented  in  Tables  9-3
through 9-5.  These are  arguably the  most
important tables  in the  entire report, but
they are not constructed  in  a manner that is
helpful to the reader looking for information
about how responses are  related  to  dose
range.   Without information about  dose
range, the effects  reported  are easily misin-
terpreted.   One fruitful  idea  might be to
start  with background exposures,  report the
most  sensitive endpoint first, and  then  order
all of the  effects along a  continuum  of  dose.
A "Comments Section" should be added to
the table to highlight key uncertainties in
the database on particular effects.

4.12.2  Evaluation of Major Conclu-
         sions  (Charge Question  1)

       Five major conclusions related to the
health effects of 2,3,7,8-tetrachlorodibenzo-p-
dioxin (TCDD  or dioxin)  are contained in
Chapter 9.  These conclusions  purport to
draw on the extensive data base  on TCDD
and related compounds  presented in Chap-
ters 1 through 7 and part of Chapter  8.

       The comments following the quotation
of each of Chapter 9's conclusions presented
below reflect the Committee's concerns with
that conclusion.

a)     "The scientific community has identi-
       fied and described a series of common
       biological steps  that are necessary for
       most (if not all)  of the observed effects
       of dioxin and related compounds in
       vertebrates, including humans.  Bind-
       ing of dioxin-like compounds to a cel-
       lular protein  called the  Ah receptor
       represents the first step in a series of
       events  attributable to   exposure  to
       dioxin-like compounds, including bio-
       chemical,  cellular,  and  tissue  level
       changes in normal biological processes.
       Binding to the Ah receptor appears to
       be necessary for all well-studied effects
       of dioxun but is not sufficient, in and of
       itself,  to  elicit these responses.   This
       reassessment concludes that the effects
       elicited by exposure to 2,3,7,8-TCDD
       are  shared  by  other  chemicals that
       have  a  similar  structure  and  Ah
       receptor-binding characteristics.  Con-
       sequently, the  biological system re-
       sponds to the cumulative exposure of
       Ah receptor-mediated chemicals  rather
       than to  the  exposure  to any  single
       dioxin-like  compound."  (Chapter 9,
       page 78)

       The  conclusion that some responses
to  TCDD  and structurally  related   com-
pounds are  initiated  by  binding to  (and
activation  of the  Ah receptor) is  clearly
supported by an extensive body of data on
many, if  not all,  relevant endpoints.   The
acceptance  that toxicities  of  potential con-
cern  are   receptor-associated  provides  the
opportunity  to  draw upon the  principles of
receptor action that  have  been developed
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                                                                            PAGE   75
and  validated  within  the  disciplines  of
immunology, biochemistry, and pharmacol-
ogy.    These principles are  not unique to
TCDD, but rather apply to all  ligand recep-
tor interactions (however,  see the discussion
in section 4.2.1 concerning the relationship
of the  binding of TCDD to the Ah receptor
and  the manifestation of  toxicity).  Recep-
tor theory provides the basis for the devel-
opment of quantitative descriptions of these
interactions  that take into account both the
affinity  of the ligand for its cognate receptor
and  efficacy.   The latter is  based  on the
experimental observation that not all ligands
produce the same  quantitative response as
a  function  of  receptor occupancy.   The
extremes range from full agonist to  antago-
nist.   Although the conclusion  that TCDD
toxicity  is  receptor-associated  is  straight-
forward  and  supported by  the data, the
broader   implications   for   environmental
mixtures that  contain  synergists, agonists,
partial agonists, and antagonists need  to be
more  fully  considered—both  qualitatively
(What compounds are present?) and quanti-
tatively (in  what amounts?).  In the overall
context of the  exposure-dose-response  para-
digm,  it  is  necessary  also  to incorporate
pharmacokinetic  data  that  will allow  a
qualitative  and  quantitative  description of
the presence of the various compounds (and
their metabolites) in potential target  tissues.
Clearly,  not all dioxin-like  compounds are
alike  in  their  metabolic stability,  tissue
distribution patterns, or biologic half-life.

       The  conclusion  that  "the  biological
system responds to the  cumulative exposure
of Ah receptor-mediated chemicals" needs to
be more specifically defined  to incorporate
the  above  concerns.   The  implication of
simple   additivity  ignores individually and
collectively  the  chemical   and  biological
properties of these chemicals as well  as the
well-established and  experimentally verified
principles of receptor-ligand interactions.

b)    "There  is adequate evidence based on
      all available  information,  including
      studies  in human  populations as well
      as  in laboratory  animals and from
      ancillary experimental data, to support
      the inference that humans are likely to
      respond with  a  broad  spectrum of
      effects  from  exposure to dioxin and
      related  compounds--if exposures are
      high  enough.  These effects will likely
      range from adaptive change at or near
      background levels  of exposure  to ad-
      verse  effects with increasing severity as
      exposure increases  above background
      levels. "(Chapter 9, page  79).

      The implication of simple
   additivity ignores individually
   and collectively the chemical
    and biological properties of
  these chemicals as well as the
         well-established and
       experimentally verified
   principles of receptor-ligand
             interactions.
      Effects and exposure levels need to be
specified.  For example, are all effects nega-
tive as implied by the term increasing severity?
Here, it is imperative that the entire database
be explicitly considered in the document, on
an end-point specific basis. For example, are
all effects negative  as implied by  the term
increasing severity? Here, it is imperative that
the entire database be  explicitly considered in
the document,  on  there  is evidence from
bioassays in rodents  that  TCDD  could be
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PAGE  76
chemoprotective   against   breast   cancer
(Kociba  et al,  1978)  -- although  not all
Members of the Committee  accept this find-
ing.  Recent studies from Seveso (Bertazzi et
al,  1993) seem to show a pattern of decreas-
ing  relative risk values  for breast cancer in
women when comparing exposure zones  R to
B to A (i.e., from lower to higher estimated
exposure).  It must be noted, however,  that
these relative  risk estimates  are  extremely
unstable, and, as is  the case with cancer
exceedences, are based on very small numbers
(e.g., one case of breast cancer in zone A).
In addition, no other  epidemiological studies
have reported such effects, so the issue of the
nature of effects remains problematical.

      The overall impact of certain biochemi-
cal  changes (e.g., increased levels of phase I
and  phase II  metabolizing enzymes) seen at
lower levels is not fully understood at this
time. Current knowledge of the mechanisms
of TCDD  toxicity has not identified  the
biological determinants  of  specificity  that
would allow  one to extrapolate toxitities
across species with confidence.

      In summary, there is  not reason nor
sufficient evidence to reject  completely the
EPA's  statement  above, but it  should be
revised to sharpen its message, better indicate
areas of uncertainty, and reflect (with appro-
priate caveats) the total extant database.

c)     "In   TCDD-exposed   men,   subtle
      changes in biochemistry and  physiol-
      ogy, such as enzyme induction, altered
      levels of circulating reproductive  hor-
      mones, or reduced glucose tolerance,
      have been detected in a limited num-
      ber of  available  studies.  These find-
      ings, coupled with knowledge derived
      from animal experiments,  suggest the
      potential  for adverse impacts on hu-
      man  metabolism and developmental
      and/or reproductive biology and per-
      haps, other effects in the range of cur-
      rent  human exposures.    Given the
      assumption that TEQ intake values
      represent   a  valid   comparison with
      TCDD exposure, some of these adverse
      impacts may be occurring at or within
      one order of  magnitude  of  average
      background TEQ intake or body-bur-
      den levels (equal  to .3-6 pg  TEQ/kg
      body weight/day or 40-60 to 600 ppt
      in  lipid).   As body burdens increase
      within and above this range, the proba-
      bility and severity as well as the spec-
      trum of  human  non-cancer  effects
      most likely increase.  It is not currently
      possible to state exactly how or at what
      levels humans  in the population will
      respond,  but the margin of exposure
      (MOE) between background levels and
      levels where effects are  detectable  in
      humans in terms of TEQs is consider-
      ably  smaller  than  previously  esti-
      mated." (Chapter 9, page 81)

      Potential   adverse  effects  are stated
without a clear definition of  the doses  or
exposure levels at which they occur.  EPA's
revision should attempt to provide a range of
exposures  linked to a range of adverse effects,
much like it has done with lead toxicity, and
incorporate  some identification as  to what
adverse effects could be expected.

      A  critical issue throughout  the risk
characterization chapter is the assumption of
the validity of the TEQ approach.  Employ-
ment of TEQs  assumes  that  all dioxin-like
compounds have equal efficacy and in the
context of  the  receptor  occupancy  theory
(i.e.,   linear  stimulus   transfer),   simple
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                                                                              PAGE  77
additivity  can be used.   Experimental evi-
dence does not support the position of equal
efficacy (which would rule out the  presence
of partial agonists or antagonists).  With the
knowledge that dioxin-like compounds have
differing receptor  affinity,  the  well-docu-
mented  difference in  the levels  of the Ah
receptor in various  tissues would determine
whether synergism  or  antagonism  would
predominate.   Finally,  in vivo,  dioxin-like
compounds differ significantly in their distri-
bution and metabolism.  Despite these caveats,
the sense of almost all of the Committee was that
the TEQ approach  is, until some better alternative
is developed, the best available vehicle for perform-
ing risk assessment involving complex mixtures  of
dioxins, furans, and co-planar PCBs.  To address
the caveats  noted  above, EPA should conduct a
peer review  of the TEF/TEQ  approach,  with
particular attention to the role of partial agonists
and antagonists.

      The last sentence  of the above quoted
EPA conclusion ("It is not currently possible
to state exactly how or at what levels humans
in the population will respond,  but the mar-
gin of exposure (MOE) between background
levels and  levels where effects are detectable
in humans in terms  of TEQs is considerably
smaller than previously estimated.") is (in the
opinion of most,  but not all of  the Commit-
tee) thought to be speculative and needs to
be reexamined.   In  effect, it  states that we
don't know what will occur, or  at what level
this unknown [response] will occur, but we
know that it will occur  (in terms of TEQs)
closer to background levels than previously
estimated.

d)    "With  regard to carcinogenicity,  a
      weight-of-the-evidence evaluation sug-
      gests that dioxin and  related  com-
      pounds (CDDs, CDFs, and dioxin-like
       PCBs are likely to present a cancer
       hazard to humans.  While major un-
       certainties remain, efforts of this reas-
       sessment to bring more data into the
       evaluation  of cancer  potency  have
       resulted in a risk-specific dose estimate
       (1 X 10"6 )  risk or one additional can-
       cer in one million exposed of approxi-
       mately   0.01   pg  TEQ/kg  body
       weight/day,  This  risk-specific  dose
       estimate  represents a plausible upper
       bound on risk based on the evaluation
       of animal and human  data.   "True"
       risks are not likely to exceed this value,
       may  be less, and may even be zero for
       some members  of the population."
       (Chapter 9, page 85)

       Existing  epidemiological methodolo-
gies,  coupled with  the available database,
simply do not  have the power  to identify
possible cancer/dioxin  links at background
levels.  Consequently,  the conclusion that
dioxin and  related compounds are likely to
present a cancer hazard to humans at expo-
sure levels within one or two orders-of-magni-
tude above background is not well-supported
by  the existing human epidemiologic data-
base.

       The  previous (and  apparently  still
current) position of the EPA has been to use
a linear extrapolation approach.   This  ap-
proach employs  TEQ additivity and, to some
extent, is contrary to the principles applicable
to  the quantitative modeling of  receptor-
mediated processes, most of which assume the
absence of a threshold.  There is a consider-
able body of peer-reviewed data, including the
bioassay data in Sprague-Dawley rats (Kociba
et al.,  1978) and more recent  studies on the
TCDD-dependent  changes  in  hepatocyte
proliferation  (Fox  et   al.,   1992)   and
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PAGE  78
preneoplastic foci  (Maronpot et al.,  1993),
which challenges both of these assumptions.
Thus, the risk-specific dose estimate  (at the
lO'6  level of risk) of 0.01 pg, TEQ/kg body
weight/day is not supported by the available
data.
      All of the above notwithstanding, the
main cancer hazard issue for EPA and other
risk managers is to identify and gain a better
understanding of those conditions of exposure
wherein dioxin can be a human carcinogen.
The Agency should revise its conclusions so as
to state background risk in terms of a range
from lower to higher, and should identify the
range of  risks  for specific exposures above
background exposures that may pose a human
hazard.

e)    "Based on all of the data reviewed in
      this reassessment and scientific infer-
      ence, a picture emerges of TCDD and
      related compounds as potent toxicants
      in  animals with the potential to pro-
      duce a spectrum of effects.  Some of
      these effects may be occurring in hu-
      mans at very low levels, and some may
      be resulting in  adverse  impacts  on
      human health." (Chapter 9, page 87)

      TCDD  is a potent animal toxicant,
producing a spectrum of effects dependent on
the dose, context of exposure, and the genetic
background. Not all responses in animals can
be classified as adverse.  In humans under
conditions of high exposure, the most well-
documented response is chloracne.  Adverse
effects attributable to chronic low level expo-
sure  have not  yet been adequately demon-
strated. The neuropsychological abnormalities
reported in the Lake Michigan (Jacobson and
Jacobson,  1994)  (as  discussed in  section
4.6.1)  and North Carolina (Gladen et al.,
1988) PCB exposure studies are suggestive,
even though the exposures (albeit at "environ-
mental levels") are probably higher than one
would consider to be near general background
levels.   The overarching  issue  here  is the
validity of extrapolation of animal data on
dioxin to humans (which assumes rodents and
humans to be equivalent in sensitivity) and
the default assumptions applied to the analy-
sis of the animal data which form the basis of
the positions taken in the  risk characteriza-
tion.

      It is difficult to determine what EPA is
inferring in the last  sentence  in the  above-
cited conclusion. If it is intended to state that
adverse effects in humans may be occurring
near current exposure levels, it is the Commit-
tee's judgement that EPA has  not presented
findings  that  support this  conclusion  ade-
quately.

4.13  Other Issues and Future Steps

      In the preceding text, the Health Panel
of the Dioxin Reassessment Review Commit-
tee responded  to  the  '.   explicit questions
contained in its charge.    -ing the course of
the Panel's deliberation    Avever, some ge-
neric issues not addressed in the 23 questions
arose. These are:

a)    The advantages and disadvantages of
      expressing ove:  1  risks  for 2,3,7,8-
      TCDD, other cL 
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                                                                                PAGE  79
mittee  nor the public commentors who  ad-
dressed this topic can propose an approach
better than that used by EPA.  The sense of
most of the  Committee, developed  as this
review was prepared, was that EPA and other
interested parties would be better served if the
risk assessments for 2,3,7,8-TCDD,  other
dioxins and furans, and co-planar PCBs were
done separately, as well for environmental mix-
tures as a  whole.27  Having these  risk assess-
ment calculations provided  side-by-side in a
single document will help risk managers, since
control  strategies  and options  may  differ
substantially for the separate categories, while
recognizing that most human exposures will
be to complex mixtures.

b)     The implications to the risk assessment
       of alternate forms for the exposure-
       response relationship at low (environ-
       mentally relevant) levels of exposure.

       The Committee urges EPA to examine
fundamental  principles of  receptor  theory,
and the evidence  from the epidemiological
and toxicological data bases in the low expo-
sure ranges  for their consistency with  its
assumption of a linear, non-threshold carcino-
genic risk.  In addition, the Committee (with
several  exceptions) believe  that the Agency
should at least consider the  suggestions from
the public28  regarding evidence for reduced
cancer risks associated with very low levels of
exposure.  Although such a concept seems to
be counterintuitive, there is a body of litera-
          Several Committee Members believe that
keeping dioxin-like PCBs together with the dioxins and
furans in a single overall reassessment is appropriate and
consistent with EPA's role of managing health risks to the
general public.
ture (albeit debatable, and both pro and con)
on the concept of hormesis and ionizing radia-
tion biological effects; this concept was not
discussed during the review  meeting, but is
mentioned as a possible area of future investi-
gation
29
c)     The  extent to  which revisions  in  the
       draft  reassessment document will be
       needed to warrant the endorsement of
       SAB in terms of the appropriate utiliza-
       tion of scientific knowledge in the prep-
       aration of the risk assessment.

       The  Committee concluded that EPA
reviews of the background and relevant litera-
ture in Chapters 1-7 were thorough and objec-
tive, and that, subject to revision to incorpo-
rate changes made in  response to specific
technical  input  from  the  Committee  and
others, no further SAB review was needed.

       By contrast, the Committee concluded
that more  substantial  revisions  would  be
needed in Chapter 8  on modeling,  and in
Chapter 9 on risk assessment, and a further
SAB public review session would be needed
before any SAB endorsement  of EPA's judg-
ments on the extent of health risk posed by
2,3,7,8-TCDD, other dioxins and furans, and
co-planar PCBs.  In view of the  major public
health and economic implications of the ulti-
mate judgments on the extent of these risks,
the Committee recommends that this further
effort be undertaken as soon as possible.
       28
          Based on an analysis of data in Fingerhut ef a/.,
1990.
       29
          Several Members of the Committee believe that
the evidence of "hormesis" for dioxin-lime compounds is not
statistically or experimentally significant at this time, and that
until more solid evidence is obtained, this issue is irrelevant.
These Members also contend that the putative "hormesis"
effects are occurring at the levels of exposure at which the
developmental and immunological alterations are seen
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PAGE  80

      The importance of this revision to the
reassessment of dioxin risks demands that the
highest standards of peer-review extend to the
risk    characterization    itself.    Although
it  can be argued that this is in fact being
carried out by this SAB Committee, submit-
ting  the  risk characterization  chapter  for
external peer review prior to final review by
the SAB would serve to strengthen the docu-
ment,  and assure a greater likelihood of its
acceptance by  the  scientific community-at-
large. It is recommended strongly that: a) the
risk characterization chapter undergo major
revision;  and  b)  the revised document be
peerreviewed by a group of preeminent scien-
tists, including some researchers from outside
thedioxin "community" before returning to
the SAB.  It is particularly important to in-
clude individuals with outstanding   creden-
tials     and  experience  in  basic  research
and quantitative modeling of receptor-medi-
ated processes, as well as other scientists with
broad  toxicological,  epidemiological,  and
public health,  perspectives in such a review.
                                                      THE SCIENCE ADVISORY BOARD

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