United States
                       Environmental Protection
                       Agency
                                Office of Exploratory
                                Research
                                Washington, DC 20460
EPA/600/M-89/003
March 1989
                       Research and Development
&EPA    ANNOUNCEMENT
Application
Receipt Date:
May 1, 1989
Request  for Applications
NPIR-003-89
                      Innovative Biodegradation Technologies for
             Treatment of Hazardous Organic Wastes at Superfund Sites
I. General Background

   Many hazardous waste sites have been placed on the
National Priority List for clean-up or remediation  under
Superfund. A wide variety of contaminants can be found at
these sites and over a period of time may enter subsurface
aquifers and endanger the nations water supplies.
   Organics are contaminants of particular concern, be-
cause large quantities occur at the sites and many are toxic
at  low concentrations and refractory  in the environment.
 lese substances include chlorinated and non-chlorinated
solvents (e.g., methylene chloride, carbon tetrachloride and
toluene used in huge quantities in industry), preservatives
and pesticides (e.g. creosote and lindane), wastes from the
manufacture of explosives such as TNT and other sub-
stances including polyaromatic hydrocarbons and polychlori-
nated biphenyls (PCBs). A common characteristic of these
substances is that their chemical properties make them very
difficult to remove from hazardous sites using current meth-
ods. The naturally occurring microorganisms at these sites
cannot  effectively degrade these toxic compounds. New
techniques are needed to accelerate biological degradation
of hazardous organics.

A. Purpose of RFA

   The purpose of this RFA is to solicit proposals that will
result in the development of cost-effective methods for biode-
gradation of hazardous organic wastes at Superfund sites.
Treatment in this announcement refers to the degradation of
organic wastes, not immobilization. The research and devel-
opment projects being solicited should result in practical end
products with the potential for treating hazardous organic
wastes at Superfund sites. Biological techniques that utilize
genetically engineered microorganisms can be included in
this solicitation but will require the proposer to provide any
special clearances needed for such projects.
                          B. Limitations

                            Hazardous organic waste treatment technologies to be
                          considered in this RFA must meet the following require
                          ments:
                          1.  The net result of the technology must be the biologica
                             degradation of the contaminants to reduce their toxicit}
                             and concentration in soil and subsurface water. Proc
                             esses in which the net result is to transfer contaminant?
                             between media or to immobilize a contaminant within i
                             single phase are not acceptable.
                          2.  Providing the technology meets the definitions in state
                             ment 1 above, pretreatment efforts such as modificatior
                             of pH, addition of nutrients  which improve the biode
                             gradability of the hazardous organic wastes are accept
                             able.
                          3.  "Pump-and-treatment" processes where organic waste
                             pollutants are treated or removed from  contaminatec
                             water or air after being brought to the surface will not be
                             considered.

                          II. Research Topics of Interest

                            Techniques which enhance the biodegradability of haz
                          ardous organic wastes at Superfund sites through pre-treat
                          ment processes are specifically solicited.
                            The topical areas of interest listed below are provided as
                          examples only and are not meant to be all inclusive:

                           1. microbiological process development
                           2. biological treatment mechanisms
                           3. treatment applications: soils  and sludges
                           4. enhancement of in situ biorestoration of  contaminatec
                             soils and subsurface water
                           5. aerobic/anaerobic biomineralization of toxic organics

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III. Special Instructions to the Applicants

A. Proposals must be for research which does not
include:
    1. large demonstration projects.
    2. state-of-the-art market surveys.
    3. preparation  of materials  and documents such as
    process designs or instruction manuals.

B. Proposals must include:
  1. Clearly stated  hypotheses and relevant experimental
    questions.
  2. Definition of data and analyses needed to scientifically
    evaluate the hypotheses and questions.
  3. No more than 30 pages (regular size type - no smaller
    than elite, single or double spaced, standard 8-1/2" x 11"
    pages), one side only, including application forms and all
    enclosures, covers or attachments. [Proposals exceed-
    ing 30 pages will not be reviewed.]
      A letter of transmittal is not necessary. If one is fur-
    nished, it must not be attached to every copy of the
    proposal. If a letter of transmittal is attached to every copy
    of the proposal, it will be counted as page 1 of the pro-
    posal.
  4. A budget of $200,000 or less the per year for the project
    period, which should not exceed two years in duration.

IV. Mechanisms of Support
   Assistance under this RFA will be through the U.S. Envi-
ronmental Protection Agency's Research Grants  Program,
and limited to non-profit research organizations and educa-
tional institutions. Responsibility for the planning, direction,
and execution of the proposed research will be solely the
applicant's. Approximately 1.5 million dollars will be available
from fiscal year 1989 funds and it is estimated that 10 to 15
proposals will be supported. This RFA is for a single compe-
tition with a deadline of May 1, 1989, for receipt of applica-
tions.
                   V. Review Procedures and Criteria

                   A. Review Procedures
                      Applications in response to this solicitation will be re-
                   viewed together on a nationwide basis. The  review will be
                   conducted by a scientific peer panel which will evaluate and
                   rank each proposal according to its scientific merit as a basis
                   for  recommending Agency  approval or disapproval. The
                   panel will be composed primarily of non-EPA  scientists who
                   are acknowledged experts in the area.

                   B. Review Criteria
                      The  applications will be evaluated using the following
                   criteria:
                   •  Quality of research plan (including  theoretical and/or
                       experimental design, originality, and creativity)
                   •  Qualifications of principal investigator and staff including
                       knowledge of subject area
                   •  Availability and adequacy of facilities and equipment

                   VI. Method  of Applying
                       Application forms, instructions, and other  pertinent infor-
                   mation are contained in the EPA Research Grant Application/
                   Information Kit. The kit is available from:

                              Mr. Donald f. Carey
                              Research Grants Staff (RD-675)
                              U.S. Environmental Protection Agency
                              401 M Street, Southwest
                              Washington, DC 20460

                       The original and eight copies of the application must be
                   received no later than close of business, May 1,1989, to be
                   considered.  The application should be sent  to the Grants
                   Operation Branch (PM-216F) at the above address.

                   VII. Staff Contact
                       Questions relating to this solicitation may be directed to
                   Mr. Donald Carey by telephone, (202) 382-7445.
 United States
 Environmental Protection
 Agency
Center for Environmental Research
Information
Cincinnati, OH 45268
  Official Business
  Penalty for Private Use $300
  EPA/600/M-89/003

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