Risk of Infectious Disease from Use of
Sludge on Land and Methods to Reduce These Risks
(U.S.) Environmental Protection Agency
Cincinnati, OH
Feb 86

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                                                     PB86-166378

                                                  EPA/600/D-86/038
                                                  February 1986
RISK OF INFECTIOUS DISEASE  FROM USE  OF  SLUDGE  ON  LAND

          AND METHODS TO REDUCE THESE RISKS
                    0.  8.  Farrell
             Wastewater Research Division
        Water Engineering  Research Laboratory
               Cincinnati, Ohio  45268
        WATER ENGINEERING RESEARCH LABORATORY
          OFFICE OF RESEARCH AND DEVELOPMENT
         U.S. ENVIRONMENTAL PROTECTION AGENCY
               CINCINNATI, OHIO  4£.?68

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                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverie before completing)
  REPORT NO.
  EPA/600/D-86/038
                                                           3. RECIPIENT'S ACCESSION NO.
                                     lbfe378/AS
4. TITLE AND SUBTITLE
   Risk of Infectious  Disease from Use of Sludge  on
       Land and Methods  to  Reduce these Risks
                                                           5. REPORT DATE
                                                               February  1986
                            6. PERFORMING ORGANIZATION CODE
 . AUTHOR(S)

       0. B. Parrel!
                                                           8.'PERFORMING ORGANIZATION REPORT NO
9. PERFORMING ORGANIZATION NAME AND ADDRESS
   Wastewater Research  Division
   Water Engineering  Research Laboratory
   U. S. Environmental  Protection Agency
   Cincinnati, OH  45268
                                                           10. PROGRAM ELEMENT NO.
                            11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
                                                            13. TYPE OF REPORT AND PERIOD COVERED
   Water Engineering  Research Laboratory,  Cin.,  OH
   Office of  Research and Development
   U.S. Environmental  Protection Agency
   Cincinnati, OH   45268
                            14. SPONSORING AGENCY CODE

                                EPA/600/14
 15. SUPPLEMENTARY NOTES
   Author:  J.  B.  Far>"ell
513-569-7645
FTS 8-684-7645
 16. ABSTRACT
            Virtually all of the  infectious organisms in wastewater end up in the
       sludge  removed in the course  of wastewater treatment.   The health risks to
       humans  when sludges are applied to land depend on the  densities of these
       organisms,  reductions that  occur when the sludge is  processed, the hardiness
       of  the  organisms on or in  the land surfaces, and the available pathway: to
       man.   This  presentation discusses each of these issues for pathogenic bacteria,
       viruses,  protozoa, and helminths.  The combination of  sludge processing require-
       ments and other controls,  such as control of access  and type of crops growing,
     .  .used  by the U.S. EPA in their regulations governing  sludge use on land are
     ^  yresented and discussed.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
               b.lDENTIFIERS/OPEN ENDED TERMS  c.  COSATI Field/Group
18. DISTRIBUTION STATEMENT


    RELEASE TO PUBLIC
               19. SECURITY CLASS (Tills Report)
                 Unclassified
                       21. NO. Of PAGES

                            15
               20. SECURITY CLASS (Thi

                 Unclassified
                                          22. PRICE
 EPA Form 2220-1 (R»v. 4-77)    PREVIOUS COITION is OBSOLETE

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                      NOTICE

This document has been reviewed in accordance with
U.S. Environmental Protection Agency policy and
approved for publication.  Mention of trade names
or commercial products does not constitute endorse
ment or recommendation for use.
                        11

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          RISK OF  INFECTIOUS DISEASE  FROM USE  OF  SLUDGE  ON  LAND
                    AND METHODS TO REDUCE THESE  RISKS

                                    by

                              J. B.  Farrell

     Wastewater from a  community is  extensively  contaminated  with  organisms
that cause infectious disease.   Primarily these  are organisms that are  dis-
charged through the intestinal  tract.  Modern  drinking water  and wastewater
systems are based  on an appreciation  of the  high  disease hazards present
in wastewater.  The organisms of concern are:  bacteria,  viruses, protozoa,
and the eggs of helminths (parasitic worms).   All  of these  materials,
even viruses, end  up in high densities  in the  sludge.   Viruses,  although
very small, are highly adsorbed or contained in  solids,  so  proportion
removed with sludge is very high.

     The questions we must ask about these organisms are the  following:

     a)  Are these organisms in sludge at infective levels?
     b)  Are they  reduced adequately by treatment?
     c)  Are there pathways that could lead  to infection in man?
     d)  Do the organism densities decline rapidly with time  when
            sludge is applied to land?

     The first part of this presentation will  discuss items a, c,  and  d
for each class of  organisms in turn.   Item b will be discussed briefly but
will be taken up in more detail later.

Bacteria
     The pathogenic bacteria of major concern which are found in sludge are
 listed in Table 1.  Symptoms of diseases produced by these organisms are dis-
 cussed elsewhere  (ref. 1).  These diseases are frequently serious and some-
 times life-threatening.  Densities of the causative bacteria are reduced
 by conventional treatment.  A substantial dose of pathogenic bacteria (many
 thousands of CFU's-colony forming units—in some cases) is generally needed
 to cause disease  and frequently densities are low enough that ingestion of
 an infectious dose seems unlikely.  However, most of these bacteria can
 regrow if provided with a nutritious substrate, so even low densities are
 of concern in certain circumstances.

     Pathways to  man include aerosols, animals, crops, drinking water from
 surface or groundwater sources, vectors, and fomites.  Results in the
 literature indicate that bacteria live on soil and crops long enough to be
 of sustantial concern (see Table 2).  The aerosol route seems to be a
 minor hazard that can be minimized by good application techniques.
 Bacteria are relatively large and are filtered by most soils, excepting
 coarse sandy soil and thin soils over fissured bed rock.  Surface runoff
 is of concern, as is transmission by crops and livestock.  Transmission
 by vectors and fomites can be minimized  by proper pretreatment of sludge
 and by good housekeeping.
                                                                  2/6/86

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                                   -2-

Vi ruses

     Human viruses  commonly found in wastewater are presented in  Table 3.
Symptoms of these diseases are discussed by Kowal  (1).
Illnesses produced  ranee from mild to extremely serious.

     Pathways to man are similar to those for bacteria,  and most  concerns
about disease transmission are similar.   Viruses survive  about as long as
bacteria on soil and plants and may survive considerably  longer when carried
deep into the soil.  Transmission via animals is possible because some
animals  can be infected by human viruses.  However, this  risk is  unproven
and may  be slight., Potential for transmission to groundwater is  greater
than for bacteria.   Viruses normally adsorb strongly to  solids including
soil granules.  However, a change in the ionic condition  of water may
cause them to desorb.  There have been reports of considerable movement
of viruses after a heavy rainfall.

     Viruses cannot regrow outside of living organisms,  so hazard of trans-
mission is reduced.  On tiie other hand, infective dose for viruses can be
very low, so caution must still be exercised when sludge containing
vi ruses i s used.

Protozoa

     Types of protozoa which may be  found  in wastewater and  sludge are
shown in  Table  4.  According to Kowal (1), only three species are of
major significance for transmission  of disease to humans through waste-
water:  Entamoeba histolytica, Giardia Iambiia, and Balantidium coli.
These organisms are hazardous when they survive wastewater treatment.
All  of  these organisms are greatly reduced in densities by sludge treat-
ment processes  and especially by exposure  on the soil.  Pathways to man
from sludge application by transport  to surface or groundwater are not
considered significant.   The primary  protective factor is  their  short
life on the soil.  Any measure  imposed to  protect against  viruses and
bacteria  will surely be protective against protozoa.  These  agents are
much more dangerous to humans when discharged with wastewater into a
receiving stream, primarily because  they can reach a swimming area or a
drinking  water  source in a matter of  hours after discharge.

Helminths

     Pathogenic helminths of major concern are listed in Table 5.  Reime^s
et al.  (2, 3) have found Ascari s, Trichuri s, and Toxocara  to  be  the most
frequently recovered helminth eggs in municipal sewage sludge in the
United  States.  Taenia saginata and  T_. solium, the beef and  pork tapeworm,
are  relatively  rare in the United States but are fairly common in some
areas of  the world.

     The  pathways to man are slightly different for helminths than for
viruses ana bacteria.  Because  of the relatively large size  of the eggs,
they are  filtered by the soil,  and it is highly unlikely that they will
reach or  travel with groundwater.  They are  relatively high  in density
and  tend  not to be carried to surface waters by runoff.  On  the  other
hand, they are  extremely  resistant to ordinary environmental  conditions
and  some  survive in the soil for periods exceeding a year.   The  soil  can

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                                   -3-  ^

present a hazard long after any evidence of sludge use has disappeared.
They can be ingested by grazing animals, by humans who eat crops  grown  on
sludge amended soil, or by children with pica (an occasionally encountered
habit of some children to consume dirt while at play).  Like viruses,
helminths and their eggs cannot multiply outside of their host.   Their
infective dose is very low—generally one egg is sufficient to produce  an
infection.  Unless the infection is massive, which -is unlikely,  the
illness produced is not severe.  Any potential  cycle of infection is to
be guarded against.  Such occurrences can take place when vegetables
supplied to a city are grown on farms irrigated with the city's  improperly
treated sewage.  Then infections of nearly all  the inhabitants of the
city becomes possible, and has occurred.

Reduction in Organism Density by Conventional Treatment

     The conventional means of treatment of wastewater solids are the
wastewater treatment process itself, anaerobic digestion at 35°C, aerobic
digestion at ambient temperatures, lime treatment to pH 12, storage in
lagoons, and drying on sand beds.  All of these processes produce sub-
stantial reductions in densities of bacterial and viral pathogens.  For
example, the sludge solids from conventional treatment (primary treatment
followed by the activated sludge process) ha«e about one-tenth the density
of  organisms of fecal origin as the incoming wastewater solids.  Anaerobic
digestion produces an additional reduction of about the same magnitude.
However, there  is  substantial and measurable survival.  The helminth eggs
are  almost unaffected, perhaps reduced by half, by processing through
digestion or even  lime treatment.  Since sludge after conventional treat-
ment still contains pathogens, it is clear that some protection must be
provided when  sludge  is used on land to minimize  risk to human health.

Further  Processing That Disinfects Sludge

     In  some cases, sludges or their products are used under conditions
where  human contact is substantial.   In these cases, sludges should
contain  no pathogenic organisms.  There are  three types of treatment
available: exposure to certain chemicals, high energy radiation, or to
temperature in  excess of  55°C  (time of exposure depends on temperature).
All  three of these treatments eliminate pathogenic bacteria and viruses;
unfortunately,  helminth eggs are extremely resistant to chemical treat-
ment.  Consequently, only high energy radiation or exposure to tempera-
tures  above 55°C reliably destroys all three classes of organisms of
concern.  The  processes available that provide these treatments are:

                   composting when temperatures exceed 55°c
                   thermophilic anaerobic digestion  (> 55°C)
                   thermophilic aerobic digestion  (>  55°C)
                   pasteurization
                   gamma  ray irradiation using radioactive isotopes
                   electron beam irradiation

Processing conditions found acceptable to the U.  S. EPA are presented in
their  regulations  which *re attached  (See Appendix  II B of  "Part  257-Criteria
for  Classification of Solid Waste Disposal Facilities and  Practices," which
is  attached).   Thermophi]ic anaerobic digestion  is not in  the EPA  listing of
acceptable processes, primarily because there was no experience in the  United
States with this process  above 49°C.  Recent research experience  indicates that

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                                   -4-   •'

55°C can be achieved.  Under this circumstance,  thermophilic  anaerobic  digestion
will qualify as a suitable process to disinfect  sludge.   Another  process  that
may eventually qualify is long term storage (in  excess  of two years)  of sludge
in lagoons in warm climates.  EPA research not yet published  indicates  promising
results.

EPA's Control Approach

     EPA's approach to control of risk of infectious disease  from sludge
utilization is contained in Paragraph 257.3-6 in the attached regulation.
EPA divided sludaa use into three classes:

     a)   If sludge is not treated after removal  from the wastewater
          processing step, it can not be used on  the land surface.

     b)   If sludge is treated by certain processes such as anaerobic
          digestion at 35°C  for 15 days, or lime treatment to pH 12, it
          can  be  used on  the soil surface under certain restrictions.
          The  restrictions require controlled access for 12 months after
          use  of  sludge,  crops for direct human consumption that can touch
          the  sludge  can  not be grown for 18 months, and grazing of animals
          whose products  are consumed by humans can not occur until one
          month after sludge application.

     c)   If  sludge is treated by a process that disinfects it and
          reduces  attraction of vectors, it can be used on the soil
          surface  with no restrictions related to  transmission of
          infectious  disease.

     These  regulations are  being considered for revision by  EPA, with  a
 proposed  revision due to be published in September of 1986.  The basic ideas
 noted  above  are  thought  to  be sound  and will probably form the structure of
 the new  regulation,  although  numerous minor changes are anticipated.
 (1 )   Kowal , N.
      78  pages,
                 REFERENCES

E., "Health Effects of Land Application of Municipal  Sludge,"
EPA-600/1-85/015, September 1985.
 (2)   Reimers, R. S., Little, M. D., Englande, A. J., Leftwich, D. B.,
      Bowman, D. C., and Wilkinson, R. F., 1981.  "Investigation of Parasites
      in Southern Sludges and Disinfection by Standard Sludge Treatment
      Processes."  EPA-600/2-81-166, U. S. Environmental Protection Agency,
      Cincinnati, Ohio.

 (3)   Reimers, R. S., Little, M.D., Englande, A. J., McDonnell, D. B.,
      Bowman, D. D., and Hughes, J. M., 1984.  "Investigation of Parasites
      in Sludges and Disinfection Techniques,"  U. S. Environmental Pro-
      tection Agency, Cincinnati, Ohio.

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                                    -5-
Table  1.  Pathogenic Bacteria of Major Concern
               Name
                                          Nonhuman Reservoir
Campylobacter jejuni
Escherichia colt (pathogenic strains)
Leptospira spp.
Salmonella paratyphi A, B. C'
Salmonella typhi
Salmonella spp.

Shigella sonnei. S. flexneri.
  S. boydii. S. dysenteriae
 Vibrio cholerae
 Yersinia enterocolitica.
  Y. pseudotuberculosis
Cattle, dogs, cats, poultry

Domestic and wild mammals, rats
Domestic and wild mammals, birds,
  turtles
Wild and domestic birds and mammals
 'Correct nomenclature: Salmonella paratyphi A, S. schottmuelleri. S. hirsch-
  feldi, respectively.


 Source:   Table  2  in Reference  1.
Table  2.  Survival Times of Pathogens on Soil and Plants
                          Soil
                                                   Plants
Pathogen
Bacteria
Viruses
Protozoa
Helminths
Absolute
Maximum
1 year
6 months
10 days
7 years
Common
Maximum
2 months
3 months
2 days
2 years
Absolute
Maximum
6 months
2 months
5 days
5 months
Common
Maximum
1 month
1 month
2 days
1 month
  Source:   Table  1  in Reference 1.

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                                  -6-
 Table 3.    Human Wastewater Viruses
      Enteroviruses
        Poliovirus
        Coxsackievirus A
      .  Coxsackievirus B
        Echovirus
        New Enteroviruses
      Hepatitis A Virus
      Rotavirus ("Duovirus," "Reovirus-like Agent")
      Norwalk-Like Agents (Norwalk, Hawaii, Montgomery County, etc.)
      Adenovirus
      Reovirus
      Papovavirus
      Astrovirus
      Calicivirus
      Coronavirus-Like Particles
 Source:   Table  10  in  Reference 1.
 Table  4.   Type* of Protozoa in Wastewater
Name
                      Protozoan Class
                                            Nonhuman Reservoir
HUMAN PATHOGENS

Enlamoeba histo/ytica

Giardia lamblia
Balantidium coli
Toxoplasma gondii
Dientamoeba fragi/is
Isnspora bell!
I. hominis

HUMAN COMMENSALS

Endolimax nana
Entamoeba coli
lodamoeba butschlii

ANIMAL PATHOGENS

Eimeria spp.
Enlamoeha spp.
Ameba

Flagellate
Ciliate
Sporozoan (Coccidia)
Ameba
Sporozoan (Coccidia)
Sporozoan (Coccidia)
Ameba
Ameba
Ameba
Isospora spp.
Sporozoan (Coccidia)
Ameba
Flagellate
Sporozoan (Coccidia)
Domestic and wild
mammals
Beavers, dogs, sheep
Pigs, other mammals
Cats
Fish, birds, mammals
Rodents, etc.
Dogs, cats, wild mammals
Dogs, cats
 Source:   Table  15  in  Reference 1.

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                                       -7-
Tabl e  5 .   Pathogenic Helminths of Major Concern
Pathogen Common Name
NEMATODES (Roundwcrms)
Enterobius Pinworm
vermicularis
Ascaris Ftoundworm
lumbricoides
A. suum Swine
roundworm
Trichuris trichiura Whipworm
Necator americanusHookworm
Ancylostoma Hookworm
duodenale
A. braziliense Cat hookworm
A. caninum Dog hookworm
Strongyloides Threadworm
stercoralis
Toxocara cam's Dog roundworm
T. cati Cat roundworm

Nonhuman
Disease Reservoir

Enterobiasis
Ascariasis
Ascariasis Pig*
Trichuriasis
Necatoriasis
Ancylostomiasis
Cutaneous larva Cat, dog*
migrans
Cutaneous larva Dog*
migrans
Strongyloidiasis Dog
Visceral larva Dog*
migrans
Visceral larva Cat*
migrans
CESTODES (Tapeworms)

Taenia saginata"   Beef tapeworm
T. solium          Pork tapeworm
Hymeno/epis nana  Dwarf tapeworm
Echinococcus      Dog tapeworm
 granulosus
£. multilocularis
Taeniasis
Taeniasis, Cysticerosis
Taeniasis             Rat,  mouse
                     Dog*
Unilocular hydatid
disease
Alveolar hydatid
disease
                                                       Dog, fox,
                                                       cat*
 "Definitive host; man only incidentally infested.
"Eggs not infective for man.

Source:   Table  17  in  Reference 1.

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