•V
              United States
              Environmental Protection
              Agency
            Office of Prevention, Pesticides
            And Toxic Substances
            (H-7508W)
September 1992
Reregistration
Eligibility Document
(RED)
                                           93-226
              Nosema locustae
                               U.S.
                   .    iitefTsrs
                   • to cede 3201
                 1 200 Pefitis
                                         Uorar

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               UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

                          WASHINGTON, D.C. 20460
    OCT  6 1992
   OFFICE OF
PREVENTION, PESTICIDES
AND TOXIC SUBSTANCES
CERTIFIED MAIL
Dear Registrant:

     I am pleased to announce  that the Environmental Protection
Agency has completed its  reregistration eligibility decision on
the microbial pesticide active ingredient Nosema locustae (N.
locustae).

     Enclosed is a Rereqistration Eligibility Document (RED) for
the microbial pesticide active ingredient N.  locustae.  The RED
is the Agency's evaluation  of  N.  locustae*s data base, its
conclusions regarding human and environmental risks associated
with the current product  uses,  and its decisions and conditions
under which uses and products  will be eligible for
reregistration.  Also enclosed is the EPA RED facts and the
Pesticide Reregistration  Handbook which provides instructions to
registrants on how to respond  to any labeling and data
requirements specified in the  RED and how to reregister products.

     This RED identifies  outstanding product specific data
requirements for end-use  products only.  These requirements are
listed on the Requirements  Status and Registrant's Response Form.
which, along with the Data  Call-In Response Form listing all of
your company's products subject to the RED, is included as an
Attachment.  Instructions for  completing both forms are contained
in the RED.  All product  specific data must be submitted and
found acceptable by EPA before a product can be reregistered.

     In addition, in order  to  be reregistered, all product
labeling must be in compliance with format and content labeling
as described in 40 CFR 156.10  and all labeling changes imposed by
Pesticide Regulatory (PR) Notices,  and any label changes imposed
by this RED.  The Pesticide Reregistration Handbook contains
detailed instructions for compliance with the RED and must be
followed carefully.

     There are several key  points to remember in preparing your
response to the RED:
                                                                RtcyctodfRecyclabie
                                                                  ti *t D*MT ttw oonlatf
                                                                  75* itcttue U>f

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Within 90 days of YourReceipt of ThisLetter

     1.   For each product which is subject to this RED, you must
complete, sign and submit the data call-in (DCZ) response forms
attached to the RED [Attachment B has forms for product specific
data].  Follow the instructions in those Attachments for
completing those forms and submit the forms to the appropriate
address specified on page 6 of the Product Reregistration
Handbook.  Note that the DCI forms for product specific data are
to be sent to the Registration Division Product Manager named at
the end of this letter.  The mailing distribution code for your
response is RED-RD-PM18.

     2.   With respect to the product specific acute toxicity
data requirements, EPA has created "batches" of products which
are toxicologically similar (Attachment D).  If your product is
listed in a "batch" you have the option of participating in the
batch.  If you wish to participate, you should consult with other
registrants in the batch and agree on who will provide data.
Carefully follow the instructions in Attachment C to assure that
one registrant is committing to provide the data while the others
offer to share in the cost of those data.  Of course, if you do
not want to join the batch, you must commit to provide the data
on your own.

     3.   No time extensions will be granted for submitting 90-
day responses.  If EPA does not receive a response for a product,
it may issue a Notice of Intent to Suspend that product.

     4.   Any requests for data waivers or time extensions to the
8-month deadline, detailed below, must be submitted as part of
your 90-day response.  Such requests will not be considered in
the 8-month response.

within Biaht Months of the Date of This Letter

     1.   For each product, you must submit a completed
Application for Registration (EPA Form 8570-1), five copies of
the label and labeling revised as specified by the RED and in
accordance with current requirements, two completed copies of the
Confidential Statement of Formula (CSF) (EPA Form 8570-4), a
completed Certification with Respect to citation of Data (EPA
Form 8570-31), and data or references to data (see item 2 below).

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     2.   You roust  submit or cite the required product specific
data as part of your commitment for reregistration.  For most
products, you will  probably be citing data which have already
been submitted to EPA.  In these cases, you must submit a list of
the studies and the corresponding EPA identifier numbers (i.e.,
accession or MRID numbers).  Before citing these studies, you
must make sure that they meet EPA'a current acceptance criteria
(Attachment £).  Be sure to follow data formatting requirements
in PR Notice 86-5.  Failure to adequately comply with the data
requirements specified in the RED may result in the issuance of a
Notice of Intent to Suspend a product.

     3.   The labeling and CSF which you submit for each product
must comply with PR Notice 91-2 (Appendix D).  That notice
requires that the amount of active ingredients declared in the
ingredient statement must be stated as the nominal concentration
rather than the lower certified limit.

     You have two options for submitting a CSF: (1) accept the
standard certified  limits (see 40 CFR 158.175) or (2) provide
certified limits that are supported by the analysis of five
batches.

     If you select  the second option, you must submit or cite the
data for the five batches along with a certification statement as
described in 40 CFR 158.175(e).

     4.   Send your Application for Registration to the
Registration Division Product Manager in the Registration
Division who is assigned to the product, Phil Button.  Use the
correct address shown on page 6 of the enclosed Product
Reregistration Handbook.  Note that the mailing distribution code
for your response is RED-RD-PM18.

     Questions on product-specific data requirements and labeling
should be directed  to the Registration Division Product Manager
for N. locustae, Phil Button at (703) 305-7690. Questions on the
generic database must be directed to Sue Rathman, the Review
Manager in the Special Review and Reregistration Division at
(703)  308-8069.

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     If necessary, the Agency will meet with registrants who have
questions about responding to the N. locustae RED; however/ only
one combined meeting will be held for all interested registrants.
If you want to meet with the Agency, you must contact Phil Button
within 30 days of your receipt of the RED.

      If there are any requests for such a meeting, the Agency
will notify all registrants who requested a meeting of the
location, date and time.  Requests for a meeting will not extend
the 90-day or 8-month response deadlines.

                              Sincerely,
                              Daniel M. Barolo, Director
                              Special Review and
                                Reregistration Division
Enclosures:
  RED for N. locustae
  DCI
  EPA RED FACTS

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REREGISTRATION ELIGIBILITY DOCUMENT

               CASE NAME:

               Nosema locustae

             ACTIVE INGREDIENT:

                Nosema locustae
        ENVIRONMENTAL PROTECTION AGENCY
           OFFICE OF PESTICIDE PROGRAMS
     SPECIAL REVIEW AND REREGISTRATION DIVISION
               WASHINGTON, D.C.

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             EPA N. locustae REREGISTRATION ELIGIBILITY TEAM

Office of Pesticide Programs:

Biological and Economic Analysis Division
Paul Guillebeau

Environmental Fate and Effects Division
Leo LaSota
William Schneider
David Bays

Health Effects Division
Esther Saito
J. Thomas McClintock
Laura Morris
Judy Smith

Registration Division
Phil Mutton
Willie Nelson
Ian Blackwell
Sami Malak

Special Review and Reregistration Division
Sue Rathman
Barbara Briscoe
Carol Stangel

Policy and Special Projects Staff
Kennan Garvey

Office of General Counsel:
Steve Wolfson

Office of Compliance Monitoring:
Jonathan Packman

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                     TABLE OF CONTENTS
                                                            Page
GLOSSARY OF TERMS AND ABBREVIATIONS

EXECUTIVE SUMMARY

I. INTRODUCTION

H. CASEOVERVDZW

     A.    CHEMICAL OVERVIEW

     B.    USE PROFILE

     C.    REGULATORY HISTORY

ffl.  SCDENCE ASSESSMENT OF N. locustae

     A.    PRODUCT IDENTITY/CHEMISTRY ASSESSMENT

     B.    HUMAN HEALTH ASSESSMENT

     C.    EXPOSURE ASSESSMENT

     D.    ENVIRONMENTAL ASSESSMENT

IV.  RISK MANAGEMENT AND REREGISTRATION DECISION

     A.    DETERMINATION OF ELIGIBILITY

     B.    ADDITIONAL GENERIC DATA REQUIREMENT

     C.    LABELING FOR MANUFACTURING-USE PRODUCTS

V. ACTIONS REQUIRED BY REGISTRANTS OF END-USE PRODUCTS

     A.    DETERMINATION OF ELIGIBILITY

          1. Product Specific Data Requirements
          2. Labeling Requirements for End-Use Products
iv

v

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2

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3

3

3

4

5

6

8

8

8

8

9

9

9
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VI.   APPENDICES
        APPENDIX A - Use Patterns Subject to Reregistration

        APPENDIX B - Generic Data Requirements for Reregistration and Data
                      Citations Supporting Reregistration

        APPENDIX C - Citations Considered to be Part of the Data Base
                      Supporting Reregistration

        APPENDIX D - PR Notice 91-2

        APPENDIX E - Pesticide Reregistration Handbook

        APPENDDC F - Product Specific Data Call-In

            Attachment A - Chemical Status Sheet

            Attachment B - Product Specific DCI Response Forms (Form A) plus
                          Instructions

            Attachment C - Requirements Status and Registrants1  Response Forms
                          (Form B) plus Instructions and PR Notice 86-5

            Attachment D - EPA Groupings of End-Use Products for Meeting Data
                          Requirements for Reregistration

            Attachment E - EPA Acceptance Criteria

            Attachment F - List of all Registrant(s) sent this DCI

            Attachment G - Cost Share/Data Compensation Forms
                          Application for Registration
                                       m

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                 GLOSSARY OF TERMS AND ABBREVIATIONS


CAS        Chemical Abstracts Service

CFR        Code of Federal Regulations

CSF        Confidential Statement of Formula

EPA        U.S. Environmental Protection Agency

FDA        Food and Drug Administration

FIFRA      Federal Insecticide, Fungicide, and Rodenticide Act

MCPA      Microbial Pest Control Agent

MRID       Master Record Identification (number). EPA's system of recording and
            tracking studies submitted to the EPA.

ppm        Parts per Million

RED        Reregistration Eligibility Document
                                       IV

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EXECUTIVE SUMMARY
       The U.S. Environmental Protection Agency (EPA) has conducted a comprehensive
review of the available scientific data and other relevant information supporting the
reregistration requirements of the microbial pesticide active ingredient Nosema locustae (N.
locustae).  EPA has concluded that the current approved uses of this microbial pesticide will
not result in  unreasonable adverse effects to humans and the environment. Therefore,  all
currently approved uses are eligible for reregistration.

       In this Reregistration Eligibility Document (RED) EPA provides a summary of the
scientific assessment of these data and information, conclusions of the risks associated  with
the current uses of this microbial pesticide, a description of these uses, and determination of
reregistration eligibility for each current use.  EPA also provides product labeling and  data
requirements that must be met by registrants to achieve product reregistration.

       Products containing N. locustae as the  active ingredient are eligible for reregistration
and will be reregistered when acceptable labeling and product specific data are submitted
and/or cited.  Before reregistering each product, the EPA is requiring that product specific
data and revised labeling be submitted by the registrants within eight months of the issuance
of this document.  After reviewing these data  and the revised labels EPA will determine
whether or not  the conditions of FIFRA 3(c)(5) have been met, that is, whether product
composition and labeling are acceptable and the product's uses will not cause unreasonable
adverse effects  to humans or the environment.  If these conditions are met EPA will
reregister the product.  Any end-use products  containing N. locustae in combination with
other active ingredients will not be reregistered until REDs are issued for all active
ingredients contained in that product.

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I.      INTRODUCTION

       In 1988, the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) was
amended to accelerate the reregistration of products with active ingredients registered prior to
November 1, 1984. The amended Act provides a schedule for the reregistration process to
be completed in nine years.  There are five phases to the reregistration process.  The first
four phases of the process focus on identification of data requirements to support the
reregistration of an active ingredient and the generation and submission of data to fulfill the
requirements.  The fifth phase is a review by the U.S. Environmental Protection Agency
(referred to as "the Agency") of all data submitted to support reregistration.

       FIFRA Section 4(g)(2)(A) states that in Phase 5 "the Administrator shall determine
whether pesticides containing such active ingredient are eligible for registration" before
calling in data on products and either reregistering products or taking "other appropriate
regulatory action."  thus, reregistration involves a thorough review of the scientific data base
underlying a pesticide's registration.  The purpose of the Agency's review is to reassess the
potential hazards arising from the currently registered uses of the pesticide; to determine the
need for additional data on health and environmental effects; and to determine whether the
pesticide meets the "no unreasonable adverse effects" criterion of FIFRA.

       This document presents the Agency's decision regarding the reregistration of N.
locustae.  The document consists of six sections.  Section I is  the introduction.  Section II
describes N. locustae, its uses, data requirements and regulatory history. Section in
discusses the human health and environmental assessment based on the data available to the
Agency.  Section IV discusses the reregistration decision for N. locustae.  Section V
discusses the reregistration requirements for N. locustae.  Section VI is the Appendices
which support this Reregistration Eligibility Document. Additional details concerning the
Agency's review of applicable data are available on request.1
          EPA's   reviews  of  data  on  the   set   of   registered  uses
considered  for EPA's  analysis may  be  obtained from the OPP  Public
Docket,  Field  Operations  Division  (H7506C),   Office  of  Pesticide
Programs,  EPA,  Washington,  DC 20460.

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II.    CASE OVERVIEW
A.    Chemical Overview

      The following active ingredient is covered by this Reregistration Eligibility
Document:

      Chemical Name:    Nosema locustae

      Office of Pesticide Programs
               Chemical Code:  117001
B.    Use Profile

      The following is information on the registered use with specific use sites and
application methods.  A detailed table of eligible uses of N. locustae is in Appendix A.

Type of Pesticide: Microbial insecticide

Mode of Action: Protozoan infection; infectious disease.  Product must be eaten by insect to
be effective.

Use sites:

      TERRESTRIAL FOOD + FEED CROP: Agricultural crops/soils (unspecified),
        vegetables.
      TERRESTRIAL FEED CROP: Rangeland.
      TERRESTRIAL NON-FOOD + OUTDOOR RESIDENTIAL: Ornamental lawns and
        turf.
      TERRESTRIAL NON-FOOD: Fencerows/hedgerows.

Pests: Grasshoppers and Mormon crickets.

Formulation Types Registered:      Bait/solid: 0.05% to 0.1 % A.I.
                                 Bait/liquid:  0.05% A.I.

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Methods and Rates of Application:

       The solid baits are applied by aircraft, ground spreader, or shaker can at rates up to
0.001 Ib A.I. per acre.  Liquid formulations and some of the solids are diluted and mixed
with bran.  The treated bran is applied at 1 Ib per acre by aircraft, ground  spreader, or
shaker can.

Use Practice Limitations: None.
C.     Regulatory History

       The first registration of a product containing N. locustae was issued May 9, 1980.
N. locustae is a microbial pesticide, made from the spores of the protozoan of the same
name. There are presently six registered products containing N. locustae.  These products
are solid bait and liquid formulations which are used to control grasshoppers  and crickets in
crop, fields, lawns and turf, and fencerows and hedgerows.
III.    SCIENCE ASSESSMENT

       EPA has reviewed the scientific data base for N. locustae primarily relying on data
submitted by the registrants and information from published literature. These are cited in
Appendix C.
A.     Product Identity/Chemistry Assessment

       The active ingredient consists of spores from the protozoan, N. locustae (Canning), a
microsporidian belonging to the family Nosematidae, which is infectious to certain
grasshoppers and crickets.  The life cycle of N. locustae includes a spore stage characterized
by spores of unicellular origin, a single sporoplasm and usually one long tubular polar
filament through which the sporoplasm emerges.  Following ingestion by a susceptible host,
N. locustae  spores penetrate the midgut epithelium, the vegetative forms replicate in the fat
body, and upon depletion of host tissue, sporulation occurs releasing viable spores.  N.
locustae infections are generally diagnosed by the presence of spores  in the fat body of
susceptible hosts.  Spores which measure 3.5-5.5^ (mean of 5.2/0 in length by 1.5-3.5/*
(mean of 2.8/x) in diameter may be ellipsoidal, slightly-bent or kidney-shaped and  refractive
to light. Triangulate and elongate megaspores (8.0/z in length) are also common.

       Based on the data and information submitted all physical and chemical properties data
have been satisfied; consequently, no additional generic product identity data will be
required.

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B.     Human Health Assessment

1.     Toxicology Data Base

       There is some preliminary evidence that N.  locustae and other similar microorganisms
are not hazardous for mammals or humans.  The toxicology data considered to support the
reregistration of N. locustae included acute studies, a 90-day feeding study, and an
intraperitoneal injection study in mice.  No adverse effects were noted in any of these
studies.  Although a pattern of clearance was not demonstrated in the acute toxicology studies
the results from the intraperitoneal study suggested that the N. locustae spores were
inactivated during passage through mice.  Such inactivation was demonstrated using tissues
and peritoneal lavage fluid from treated mice which, upon bioassay, did not produce the
characteristic symptoms of the disease in grasshoppers.  The subchronic and chronic studies
with microbial pest control agents (MCPAs)  are required when acute exposure studies
indicate unusual persistence of the active microbial ingredient in the tissues, organs, or body
fluids of the test animal, in the absence of any signs of toxicity or pathogenicity.  Therefore,
the lack of pathogenicity and toxicity in the 90-day feeding study with AT. locustae indicate
that even if unusual persistence were occurring, repeated dosing would not result  in an
adverse outcome.  It is  more likely, however, that  the lack of adverse effects in the 90-day
study support also the lack of persistence of  N. locustae in the test animal.

       Additional studies in  support of the toxicology data base included injection of rabbits
with AT. locustae by the intracerebral or intraocular routes which did not result in any
detectable illness at the clinical, gross or histological levels. Based upon review of the
submitted data base all relevant toxicology requirements have been satisfied.

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       A summary of the toxicology data base is provided below.  The Guideline Reference
Numbers are as specified in  Subdivision M of the Pesticide Testing Guidelines (October,
1982).

                                      Toxicology Data Base

       Guideline            Study                Results                Acute Toxicology
       Number                                                            Category

       152A-10       Acute Oral IDs, (rat)            > 2.3x10' spores               IV
       152A-11      *Acute Dermal LD» (g. p.)        > 2.3x10'spores               IV
       152A-12       Acute Inhalation (rat)            10.2 mg/L M locustae          IV
       152A-13      *Primary Eye Irrit. (rabbit)        2.29xlOV0.1 ml                IV
       152A-14      'Primary Dermal Irrit. ID,,,       > 2.3x10' spores               IV
       152A-15      'Delayed Hypersensit. (g.p.)       Approx. 107 spores/inj.       Sensitizer
                     Intraperitoneal (mice)            No Adverse Effects          Acceptable
       152A-20      *90-Day Feeding (rat)            No Adverse Effects        Supplementary**
       152B-33       *Intracerebral (mice/rabbits)        2.9xlO'/ml
       152B-33       'Intraocular (mice/rabbits)         2.9xlO'/ml

       •Currently, not required to support the registration of the TGAI of a microbial pesticide.

       •'Classified as Supplementary because the spore dose level in the diet was not provided.
C.     Exposure Assessment

1.     Dietary Exposure

       N. locustae is exempted from the requirement of a tolerance for residues in or on all
raw agricultural commodities (40 CFR 180.1041).  Such an exemption was based on the
toxicology data base, the long and safe historical use of the MFC A, and the rapid
inactivation of the microorganism by light and temperature. Residue  studies therefore are
not required.

2.     Occupational and Residential Exposure

       In Section n the Agency provided a brief description of the types of product
formulation, application methods and sites.  The technical grade material or liquid
concentrate is formulated onto a wheat bran bait which is applied by ground equipment to
cole crops,  orchards, forests, lawn and gardens for consumption by the target pests
(susceptible grasshoppers and crickets).  Although small amounts of N.  locustae will become
free from the bran  bait, this amount is considered insignificant when compared to exposure

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during manufacturing.  However, the efficacy of the product depends upon rapid ingestion of
the protozoan on the bait and the ability of the microorganism to replicate in the target pest.
Following application of N. locustae most, if not all, of the bait will be on the soil surface
and not available on the crop prior to harvest.

       Based on the application methods, significant dermal and inhalation exposure to the
mixer/loader/applicator exists, i.e. ground boom applications to row crops. However, due to
the lack of lexicological concerns for N. locustae, no exposure data are required at this time.
3.
Risk Characterization
       The potential risks to human and/or mammals from both nondietary/dietary and
occupational exposures are considered insignificant as supported by the existing toxicology
data base.  Such data clearly demonstrated the lack of detectable dose-related effects at the
clinical, gross or histological levels and the inability of the microorganism to replicate or
accumulate in the tissues of the treated animals.  In addition, N.  locustae has not been shown
to replicate at temperatures above 35°C or able to infect and/or replicate in warm blooded
animals. Although N. locustae was a sensitizer when injected into guinea pigs, a dermal
sensitization  study is no longer required for microbial pest control agents because it is
assumed that injection of foreign proteins is likely to elicit a response and that dermal
application would elicit no response.  The reporting of any allergic reactions following
exposure is currently required  by the revised  1989 Microbial Pesticide Guidelines
(Subdivision M of the FIFRA Testing Guidelines, NTIS PB  89-211676).
D.     Environmental Assessment
1.
Environmental Fate
       Since there are no ecological effects concerns from this naturally-occurring
microorganism, no environmental fate data are required.  These studies are in Tier II and are
only needed if there are significant adverse effects seen in the Tier 1 ecological effects
studies.
2.
Ecological Effects
       Sufficient studies were received to perform an ecological hazard assessment.
Although not all the studies were performed in accordance with the revised 1989 Microbial
Pesticide Guidelines (Subdivision M of the FIFRA Testing Guidelines, NTIS PB 89-211676),
(e.g. few controlled studies were carried out long enough to detect subchronic infection or
delayed pathogenicity), the Agency would not expect any adverse non-target effects from this
protozoan.  No adverse effects have been reported from the many years of experience with
environmental releases of this microorganism. N. locustae has been tested and studied for 20
years, and has been registered and used in the field  since 1980.  Considering this experience,
in conjunction with acceptable acute studies, the Agency foresees no significant adverse

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effects on nontarget species or the environment from the registered uses of N. locustae.

                               Ecological Effects Data Base
    Data
Requirements
154-16
Avian Acute Oral
               Test     Effects
              Substance
Citation         Fulfills         Waiver
               Guideline       for Data
              Requirements?     Granted?
Quail

Pheasant
Duck

Avian injection
Pheasant
154-19
Fish
TicutLCjo
Trout LCjo
TGAI

TGAI
TGAI


TGAI


TGAI
TGAI
LD50 =
> 2,000 mg/kg
no mortality
no mortality


no mortality


no mortality
no mortality
247757

408143-04
Patuxent
Wildlife

408143-05


408143-06
247757
no (core-acute)

no (supplemental)
no (supplemental)


no (supplemental)


yes (core)
yes (core)
yes

N/A
yes


N/A


N/A
N/A
154-20
Aquatic Invertebrate
 Daphnia magna

154-24
 Honeybee

154-22
 Non-target Plant

154-23  .
 Non-target Insect
              TGAI     no mortality        247757       no (supplemental)     yes
              TGAI     no mortality        article        no (supplemental)    . yes
                                          none submitted   no
                                          none submitted   no
                                   yes
                                   yes
3.
Environmental and Ecological Risk Assessment
       These studies show that N.  locustae should not have an adverse effect on avian
species, aquatic invertebrates, and  honeybees on an acute basis. The lack of adverse effects
reported from the many years of use and study of N.  locustae in the field allows for
sufficient confidence to waive the subchronic infection and delayed pathogenicity data
requirements.   In addition to the above studies, no pathogenicity or toxicity was documented
to freshwater trout.

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       No beneficial nontarget insect studies were submitted.  It is unlikely that nontarget
insects would be exposed to N. locustae at greater than naturally-occurring levels since the
spores and mycelium are formulated on wheat bran bait and applied directly to areas where
grasshopper infestation occurs, preferably when the grasshoppers are still nymphs.  These
studies are waived.  Nontarget plant studies were not submitted. These studies are also
waived since the Agency is not aware of any association of N. locustae with plant diseases
despite extensive analysis of agricultural diseases  by academia, government and industry.
IV.
RISK MANAGEMENT AND REREGISTRATION DECISION FOR N. Locustae
A.     Determination of Eligibility

       Section 4(g)(2)(A) of FIFRA requires the Agency to determine, after consideration of
relevant data concerning an active ingredient, whether products containing the active
ingredient are eligible for reregistration.  The Agency has completed its consideration of
these data and other factors and has determined this information is sufficient to support
reregistration of products  containing N. locustae as an active ingredient.  The reregistration
of particular products is addressed in Section V of this document.

       Although the Agency has concluded that products containing N. locustae are eligible
for reregistration, the Agency may take regulatory actions in the future that would affect the
continued registration of N. /ocitffoe-containing products if significant information about this
active ingredient and/or its products comes to the Agency's attention. Such regulatory action
could include requiring  the submission of additional data if the data requirements  for
registration  (or the guidelines for generating such data) change.
B.
Additional Generic Data Requirements
       The generic data base supporting the reregistration of products containing N. locustae
has been reviewed and determined to be substantially complete for reregistration.  No further
generic data are required.

C.     Labeling Requirements For Manufacturing-Use Products Of N.  locustae

       No manufacturing-use products are registered.
                                            8

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V.
ACTIONS REQUIRED BY REGISTRANTS OF END-USE PRODUCTS
A.     Determination Of Eligibility

       Based on the reviews of the generic data for the active ingredient N. locustae, the
products containing this active ingredient are eligible for reregistration. Section 4(g)(2)(B) of
FIFRA calls for the Agency to obtain any needed product-specific data regarding the
microbial pest control agents after a determination of eligibility has been made.  The Agency
will review these data when they have been submitted and/or cited and determine whether to
reregister individual products.
1.
Product Specific Data Requirements
       The Agency is requiring certain product chemistry and toxicology studies to be
submitted for end-use products. These specific data requirements are stated in Attachment C.
2.
Labeling Requirements For End-Use Products
       The labels and labeling of all products must comply with the Agency's current
regulations and requirements. Follow the instructions in the Product Reregistration
Handbook with respect to labels and labeling.

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        APPENDIX A
Use Patterns Subject to Reregistration

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-------

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             APPENDIX B

Generic Data Requirements for Reregistration and
    Data Citations Supporting Reregistration

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                              GUIDE TO APPENDIX B
   Appendix B contains listings of data requirements which support the reregistration for the
pesticide covered by this Reregistration Eligibility Document.

   Appendix B contains generic data requirements that apply to the pesticide in all products,
including data requirements for which a "typical formulation" is the test substance.

   The data tables generally are organized according to the following format:

   l.Daja Requirement (Column 1).  The data requirements are listed in the order in which
they  appear in 40 CFR Part 158.  The reference numbers accompanying each test refer to the
test protocols set out in the Pesticide Assessment  Guidelines, which are available from the
National Technical Information Service, 5285 Port Royal Road, Springfield, VA  22161.

   2.Bibliographic citation (Column 2).  If the EPA has acceptable data in its files, this
column lists the identifying number of each study. This normally is the Master Record
Identification (MRID) number, but may be a GS  number if no MRID number has been
assigned.  Refer to the Bibliography Appendices for  a complete citation of the study.

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                         APPENDIX B

       Generic Data Supporting Guideline Requirements for
                  Reregistration of N.  locustae
Requirement
Citation
PRODUCT CHEMISTRY
151 A- 10 Product Identity

15 1 A- 1 1 Manufacturing Process

151 A- 12 Discussion of Formation of
Unintentional Ingredients

151A-13 Analysis of Samples

15 1 A- 15 Certification of Limits

151A-16a Color
151A-16b Physical State
151A-16c Odor
151A-16d Density or sp. Gravity
151A-16e Ph
151A-16f Stability
151A-16g Storage Stability
151A-16h Viscosity
151A-16i Miscibility
151A-16J Corrosion Characteristics
TOXICOLOGY
152A-10 Acute Oral Toxicity/Pathogenicity

152A- 1 1 Acute Dermal Toxicity

152A-12 Acute Pulmonary Toxicity/Pathogenicity

152A-13 Acute Intravenous Toxicity/Pathogenicity




42243201
42245801
42243201
42245802

42243201
42245803
42243202
42245804
42243202
42245804
42245805
42245805
42245805
42245805
42245805
42245805
42245805
42245805
42245805
42245805

74175
135114
72638
42245806
74176
42245807
141791
42245808
42245809
42260101

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152A-14  Primary Eye Irritation/Infection

152A-15  Hypersensitivity Incidents
                                                   42245810
                                                    42260101
                                                    42245811
ECOLOGICAL EFFECTS
154A-16  Avian Acute Oral
            Pheasant
            Duck & Pheasants

154 A- 17  Avian respiratory
            Pheasant

154A-19  Freshwater fish
            Trout
   t
154A-20  Aquatic Invertebrate
            Daphnia magna

154A-24  Honeybee

154A-22  Non-target Plant

154A-23  Non-target Insect
                                                    42245812
                                                    94269008
                                                    42245813


                                                    42245814


                                                    423 1490 1

                                                    42245815

                                                      waived

                                                      waived
ENVIRONMENTAL FATE

EPA waived 40 CFR Part 158 requirements for reasons discussed in section HI.

RESIDUE CHEMISTRY

EPA waived 40 CFR Part 158 requirements for reasons discussed in section HI.

HUMAN EXPOSURE

EPA waived 40 CFR Part 158 requirements for reasons discussed in section HI.


The citations listed in the bibliography (Appendix C) were used to support these decisions.

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             APPENDIX C


Citations Considered to be Part of the Data Base
        Supporting the Reregistration

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                               GUIDE TO APPENDIX C

1.     CONTENT OF BIBLIOGRAPHY.  This bibliography contains citations of all studies
       considered relevant by EPA in arriving at the positions and conclusions stated
       elsewhere in the Reregistration Eligibility Document. Primary sources for studies in
       this bibliography have been the body of data submitted to EPA and its predecessor
       agencies in support of past regulatory decisions.  Selections from other sources
       including the published literature, in those instances  where they have been considered,
       will be included.

2.     UNITS OF ENTRY.  The unit of entry in this bibliography is called a "study".  In
       the case of published  materials, this corresponds closely to an article. In the case of
       unpublished materials submitted to the EPA the EPA has sought to identify documents
       at a level parallel to the published article from within the typically larger volumes in
       which they were submitted. The resulting "studies"  generally have a distinct title (or
       at least a single subject), can stand alone for purposes of review, and can be described
       with a conventional bibliographic citation.  The EPA has attempted also to unite basic
       documents and commentaries upon them, treating them as a  single study.

3.     IDENTIFICATION OF ENTRIES.  The entries in this bibliography  are sorted
       numerically by Master Record Identifier, or MRID number.  This number is unique
       to the citation, and should be used at any time specific reference is required.  It is not
       related  to the six-digit "Accession Number" which has been used to identify volumes
       of submitted studies; see paragraph 4(d)(4) below for further  explanation.  In a few
       cases, entries added to the bibliography late in the review may be preceded by a nine-
       character temporary identifier. These entries are listed after all  MRID entries.  This
       temporary identifier number is also to be used whenever specific reference is needed.

4.     FORM OF ENTRY.  In addition to the Master Record Identifier (MRID), each entry
       consists of a citation containing standard elements followed, in the case of material
       submitted to EPA, by a description of the earliest known submission. Bibliographic
       conventions used reflect the standards of the American National  Standards Institute
       (ANSI), expanded to provide for certain special needs.

       a.     Author.  Whenever the EPA could confidently identify one, the EPA has
             chosen to show a personal author.  When no individual was identified, the
             EPA has shown an identifiable laboratory or testing facility as author. As a
             last resort, the EPA has shown the first submitter as author.

       b.     Document date. When the date appears as four digits with no question marks,
             the EPA took it directly from the document.  When a four-digit date is
             followed by a  question mark the bibliographer deduced the date from evidence
             in the document.  When the date appears as (19??), the EPA  was unable to
             determine or estimate the date of the document.

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c.      Title.  In some cases, it has been necessary for EPA
       bibliographers to create or enhance a document title. Any such editorial
       insertions are contained between square brackets.
d.     Trailing parentheses.  For studies submitted to the EPA in the past, the trailing
       parentheses include (in addition to any self-explanatory text) the following
       elements describing the earliest known submission:

       (1)    Submission date.  The date of the earliest known submission appears
             immediately following the word "received,"

       (2)    Administrative number.  The next element, immediately following the
             word "under," is the registration number, experimental use permit
             number, petition number, or other administrative number associated
             with the earliest known submission.

       (3)    Submitter.  The third element is the submitter, following the phrase
             "submitted by."  When authorship is defaulted to the submitter, this
             element is omitted.

       (4)    Volume Identification (Accession Numbers).  The   final element in the
             trailing parentheses identifies the EPA accession number of the volume
             in which the original submission of the study appears. The six-digit
             accession number follows the  symbol "CDL," standing for "Company
             Data Library."  This accession number is in turn followed by an
             alphabetic suffix which shows the relative position of the study within
             the volume. For example, within accession number 123456, the first
             study would be 123456-A; the second, 123456-B; the 26th,  123456-Z;
             and the 27th, 123456-AA.

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                              OFFICE OF PESTICIDE PROGRAMS
                         REREGISTRATION ELIGIBILITY DOCUMENT
                                        BIBLIOGRAPHY
42243201     Vinje. E. (1992) Product Identity and Composition:  Nosema locustae.  Unpublished
                 study prepared by Bozeman Bio-Tech. 4 p.

42243202     Vinje, E. (1992) Analysis and Certification of Product Ingredients: Nosema Locustae.
                 Unpublished study prepared by Bozeman Bio-Tech, Inc. 3 p.

42245801     Merrill,  L. (1992) Product Identity and Disclosure of Ingredients: Nosema locustae.
                 Unpublished study prepared M & R Durango Inc.  8 p.

42245802   •  Merrill, L. (1992) Manufacturing Process: Nosema locustae. Unpublished study prepared
                 by M & R Durango Inc.  10  p.

42245803     Rainnie, D.  (1988)  Bacterial and Fungal Contaminants of  Nosema locustae Spore
                 Suspension: Lab Project Number:   R-86-0018.  Unpublished study prepared by
                 University of Saskatchewan, Tox. Research Center.   36 p.

42245804     Merrill,  L. (1992) Analysis of Samples and Certification of Limits: Nosema locustae.
                 Unpublished study prepared by M & R Durango Inc. 37 p.

42245805     Merrill,  L. (1992) Physical and Chemical Properties:  Nosema locustae.  Unpublished
                 study prepared by M & R Durango Inc.  16 p.

42245806     Rainnie, D.  (1988)  Acute Dermal Infectivity of Technical  Grade Nosema locustae
                 Canning  Spore  Suspensions to Sprag-ue-Dawley  Rats:  Lab Project Number:
                 R-86-0018.  Unpublished study prepared by Univ. of Saskatchewan, Tox. Research
                 Center. 38 p.

42245807     Rainnie, D. (1988) Acute Respiratory Infectivity of Technical Grade  Nosema locustae
                 Canning  Spore  Suspensions to Sprague-Dawley Rats:   Lab Project Number:
                 R-86-0018.  Unpublished study prepared by Univ. of Saskatchewan, Tox. Research
                 Center. 45 p.

42245808     Rainnie, D. (1991) Study of Intraperitoneal Infectivity and Toxicity of Nosema locustae
                 Spore Suspension in CD-I Mice:  Lab Project Number:  R-86-0018.  Unpublished
                 study prepared by Univ.  of Saskatchewan,  Tox. Research Center.  82 p.

-------

-------
^2245809    Shadduck,   J.   (1980)   Maximum   Challenge  Safety   Tests   of   Nosema
                 locustae in Mice and Rabbits:  Lab Project Number.  Unpublished study prepared
                 by Southwestern Medical School.  55 p.

 42245810    Fink, R. (1973) Eye Irritation-Rabbits Nosema locustae Spore in Saline Suspension:
                 Final Report:  Lab  Project  Number: 183-195.   Unpublished study prepared by
                 Hazleton Laborator

 42245811    Rainnie, D. (1988) Investigation of the Development of Delayed Hypersensitivity of
                 Technical Grade Nosema locustae Canning Spor Suspensions in Albino Guinea Pigs:
                 Lab Project Number:   R-86-0018. Unpublished study prepared by  Univ. of
                 Saskatchewan, Tox.  Research Center.  35 p.

 42245812    Rainnie, D. (1988) Acute Oral Infectivity of Technical Grade Nosema locustae Canning
                 Spore  Suspension  to  Ring-necked  Pheasant  Chicks:   Lab  Project  Number:
                 R-86-0018. Unpublished study prepared by Univ. of Saskatchewan, Tox. Research
                 Center. 50 p.

 42245813    Rainnie, D. (1988) Maximum Hazard of Infectivity of Technical Grade Nosema locustae
                 Canning  Spore Suspension  to  Canning-necked Pheasant Chicks:  Lab Project
                 Number: R-86-0018. Unpublished study prepared by Univ. of Saskatchewan, Tox.
                 Research Center.  79 p.

 42245814    Rainnie, D. (1988) Maximum Hazard of Infectivity of Technical Grade Nosema locustae
                 Canning  Spore Suspension to Rainbow Trout Fingerlings:  Lab Project Number:
                 R-86-0018. Unpublished study prepared by Univ. of Saskatchewan, Tox. Research
                 Center. 59 p.

 42245815    Menapace, D.; Sackett, R.; Wilson, W. (1977) Adult honey bees are not susceptible to
                 infection by Nosema locustae.  Journal of Economic Entomology 71(2):304-306.

 42260101    Shadduck, J.  (1992) Maximum Challenge Safety Tests of Nosema locustae in Mice and
                 Rabbit. Unpublished study prepared by Southwestern Medical School.  51 p.

 42314901    Bozeman Bio-Tech, Inc.  (1992) The Acute Toxicity of Nosema locustae Canning Spores
                 to  Daphnia magna  Straus Maximum  Level  Testing: Lab  Project   Number:
                 10058-DM.  Unpublished study prepared by Biospherics, Inc. 31 p.

 94269001    Vinje, R. (1992) Bozeman Bio Tech. Phase 3 Summary of MRJD 00074175. Acute  Oral
                 - Rats. Prepared by Hazleton Laboratories, Inc. 5 p.

 94269002    Vinje, E. (1992) Bozeman  Bio Tech. Phase 3 Summary of MRID
                 00072638.  Acute Dermal Toxicity - Guinea Pigs.  Prepared by
                 Hazleton Laboratories, Inc. 6 p.

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APPENDIX D





 PR Notice 91-2

-------

-------
                 UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
                            WASHINGTON, D.C.  20460
                           PR NOTICE 91-2
                                             OFFICE Of
                                          PESnCOCS AND TOXIC
                                            SUBSTANCES
NOTICE TO MANUFACTURERS, PRODUCERS, FORMULATORS,
         AND REGISTRANTS OF PESTICIDES
 ATTENTION:   Persons Responsible for Federal Registration of
             Pesticide Products.

 SUBJECT:     Accuracy of Stated Percentages for Ingredients
             Statement
 I.    PURPOSE:
      The purpose  of this  notice  is to  clarify the Office  of
 Pesticide Program's  policy  with  respect to  the  statement  of
 percentages   in  a  pesticide's  label's   ingredient  statement.
 Specifically,  the  amount  (percent  by weight)  of  ingredient(s)
 specified in the ingredient statement on the  label must be stated
 as the nominal concentration of such ingredient (s), as that term is
 defined   in   40  CFR 158.153 (i).   Accordingly,   the  Agency  has
 established  the nominal concentration as the only acceptable label
 claim for the amount of  active ingredient  in  the product.

 II.   BACKGROUND
                                       •
      For some time the Agency has accepted two different methods of
 identifying  on  the  label  what  percentage  is  claimed  for  the
 ingredient(s)  contained  in a pesticide.  Some applicants claimed a
 percentage which represented  a level between the upper and  the
 lower certified  limits.    This was  referred to  as the  nominal
 concentration.   Other applicants claimed  the lower  limit  as  the
 percentage of  the ingredient (s) that would  be  expected to  be
 present  in their product at the end of the product's shelf-life.
 Unfortunately,  this led to a  great deal  of confusion among   the
 regulated industry, the regulators,  and the consumers as to exactly
 how much of a given ingredient was in a given product.  The Agency
 has established the nominal concentration as the  only acceptable
 label claim  for the amount  of active ingredient  in the product.

     Current regulations require that the parcantage listed in the
 active ingredient statement be as precise  as possible reflecting
good  manufacturing practices  40  CFR 156.10 (g) (5). The  certified
limits  required  for  each  active  ingredient  are  intended  to
encompass any such "good manufacturing practice** variations 40 CFR
158.175{c)(3).
                                                           Printed on (bey** Piper

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-------
                               3.
 XV.  PRODUCTS THAT REQUIRE EFFICACY DATA

      All pesticides are required to be efficacious.  Therefore,
 the certified lover limits nay not  be lover then the minimum
 level to achieve efficacy.  This is extremely  important for
 products vhich are intended to control pests which threaten the
 public health, e.g., certain antimicrobial  and rodenticide
 products.  Refer to 40 CFR 158.640.

      Zn those cases vhere efficacy  limits have been established,
 the Agency will not accept certified lover  limits vhich are belov
 that level for the shelf life of the product.

 V.  COMPLIANCE SCHEDULE

      As described earlier, the purpose of this Notice  is to make
 the registration process more uniform and more manageable for
 both the agency and the regulated community.   Zt is the Agency's
 intention to implement the requirements  of  this notice as
 smoothly as possible so as not to disrupt or delay the Agency's
 high priority programs,  i.e.,  reregistration,  nev chemical, or
 fast track (FZFRA section 3(c)(3)(B).  Therefore,
 applicants/registrants are expected to comply  with the
 requirements of this Notice as follows:

           (1)   Beginning July 1,  1991, all  nev product
                registrations submitted to the  Agency
                are to comply with the requirements of  this
                Notice.

           (2)   Registrants having products  subject to
                reregistration under  PZFRA section 4(a) are to
                comply with the requirements of this Notice vhen
                specific  products  are called in by the  Agency
                under Phase V of the  Reregistration Program.

           (3)   All other products/applications that are
                not subject to (1) and (2) above vill have until
                July 1,  1997,  to comply with this Notice.
                Such applications  should  note "Conversion
                to  Nominal Concentration" on the application
                form. These types  of  amendments vill not be
                handled as "Past Track" applications but
                vill be handled as routine requests.

VI.  FOR FURTHER INFORMATION
     Contact Tyrone Aiken for information or questions concerning
this notice  on (703) 557-5024.
                                Anne E. Lindsay, Director
                                Registration Division  (H-7505

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-------
      APPENDIX E
Pesticide Reregistration Handbook

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-------
     APPENDIX F
Product Specific Data Call-in

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-------
                UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
                           WASHINGTON, D.C. 20490
                       DATA CALL-IN NOTICE


                                                       KSTCOCS AND TOXIC
 CERTIFIED MAIL                                          SUBSTANCES
 Dear Sir or Madam:


 This Notice requires you and other registrants of pesticide
 products containing the active ingredient identified in
 Attachment A of this Notice, the Data Call-in Chemical Status
 Sheet.  to submit certain product specific data as noted herein to
 the U.S.  Environmental Protection Agency (EPA, the Agency).
 These data are necessary to maintain the continued registration
 of your product(s)  containing this active ingredient.  Within 90
 days after you receive this Notice you must respond as set forth
 in Section III below.  Your response must state:

      1.  How you will comply with the requirements set forth in
         this Notice and its Attachments A through G; or

      2.  Why you believe you are exempt from the requirements
         listed in this Notice and in Attachment C,
         Requirements Status and Registrant's Response Form, (see
         section III-B); or

      3. Why you believe EPA should not require your submission
         of product  specific data in the manner specified by this
        Notice (see section III-D).

      If you do not  respond to this Notice,  or if you do not
 satisfy EPA that you will comply with its requirements or should
 be  exempt or excused from doing so, then the registration of your
 product(s)  subject  to this Notice will be subject to suspension.
 We  have provided a  list of all of your products subject to this
 Notice in Attachment B, DataCall-In Response Form, as well as a
 list  of all  registrants who were sent this Notice (Attachment F).

     The  authority  for this Notice is section 3(c)(2)(B) of the
 Federal Insecticide,  Fungicide and Rodenticide Act as amended
 (FIFRA),  7 U.S.C. section 136a(c)(2)(B).   Collection of this
 information  is  authorized under the Paperwork Reduction Act by
OMB Approval  No.  2070-0107 (expiration date 12-31-92).
                                                           Prmt on ftfcrcwa Pv*'

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       This Notice is divided into six sections and seven
 Attachments.  The Notice itself contains information and
 instructions applicable to all Data Call-In Notices.   The
 Attachments contain specific chemical information and
 instructions.  The six sections of the Notice are:
      Section Z
      Section ZZ
      Section ZZZ
            - Why You Are Receiving This Notice
            - Data Required By This Notice
            • Compliance With Requirements Of This
              Notice
Section IV  * Consequences Of Failure To Comply With
              This Notice
Section V   - Registrants' Obligation To Report
              Possible Unreasonable Adverse Effects
Section VI  — Inquiries And Responses To This Notice

The Attachments to this Notice are:
A
B
C
D

£ -
F
6
           Data Call-in Chemical Stafojs Sheet
           pqfra .Call-Iq Response Fora
           Requirements S^a,tuq  and Registrant's Response
           E.PA Grouping of fold -Use Produces , for Meeting Acute
           Toxicology Qata Requirements for Re registration
           EP Acetance
                                                 Prut
          Lj. st pf Registrar^? Receiving .Tfeis Notice
          Cost Share and J>ata' Compensation Forms, and
          Specific Data Report Form
SECTION I.  WHY YOU ARE RECEIVING THIS NOTICE

     The Agency*has reviewed existing data for this active
ingredient and reevaluated the data.needed to support continued
registration of the subject active.ingredient.  The Agency has
concluded that the only additional data necessary are product
specific data.  No additional generic data requirements are being
imposed.  You have been sent this Notice because you have
product(s) containing the subject active ingredient.

SECTION II.  DATA REQUIRED BY THIS NOTICE

II-A.  DATA REQUIRED

     The product specific data required by this Notice are
specified in Attachment C, Requirements status andRegistrant's
Response Fern.  Depending on the results of the studies required  in
this Notice, additional testing may be required.

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 II-B.  ffc^WM pqp SUBMISSION OF DATA

      You are required to submit the data or otherwise  satisfy the
 data requirements specified in Attachment C, Requirements Status
 and Registrant's Response Fora, within the timeframes  provided.


 II-C.  TESTING PROTOCOL

      All studies required under this Notice must be conducted in
 accordance with test standards outlined in the  Pesticide Assessment
 Guidelines for those studies for which guidelines have been
 established.

      These EPA Guidelines are available from the National Technical
 Information Service (NTIS) , Attn: Order Desk, 5285 Port Royal Road,
 Springfield, Va 22161 (tel: 703-487-4650).

      Protocols approved by the Organization for Economic
 Cooperation and Development (OECD)  are also acceptable if the OECD-
 recommended test standards conform to those specified  in the
 Pesticide Data Requirements regulation (40 CFR  f 158.70).  When
 using the OECD protocols, they should be modified as appropriate so
 that the data generated by the study will satisfy the  requirements
 of 40 CFR S 158.   Normally, the Agency will not extend deadlines
 for complying with data requirements when the studies  were not
 conducted in accordance with acceptable standards.  The OECD
 protocols are available from OECD,  1750 Pennsylvania Avenue N.W. ,
 Washington, D.C.  20006.

      All new studies and proposed protocols submitted  in response
 to this Data Call-In Notice must be in accordance with Good
 Laboratory Practices [40 CFR Part 160. 3 (a) (6) ].
 II-D.  REGISTRANTS RECEIVING PREVIOUS  SECTION  3fcW2WB^ NOTICES
       ISSUED BY THE AGENCY

       Unless otherwise noted herein,  this Data Call-In does not  in
 anv way  supersede or chance the requirements of anv previous Data
 Call-ln.jt.fi.  or any* other agreements  entered into with the Agency
 pertaining to such prior Notice.  Registrants  must comply with the
 requirements of all Notices to avoid issuance  of a Notice of Intent
 to Suspend their affected products.


 SECTION  III.   CQjlPLIANeE WITH REQUIREMENTS OF  THIS NOTICE

 III-A.   SCHE FOR RESPONDING TO TE AGENCY
     The appropriate responses  initially required by this Notice
for product specific data must  be  submitted to the Agency within  90
days after your receipt  of this Notice.  Failure to adequately
respond to this Notice within 90 days of your receipt will be a
basis for issuing a Notice of Intent to Suspend  (NOIS) affecting

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 your products. This and other bases for issuance of HOIS due to
 ^failure to comply with this Notice are presented in Section IV-A
 mnd IV-B.

 III-B.  OPTIONS FOR RESPONDING TO THE ACEMCY

     The options for responding to this Notice for product specific
 data are: (a) voluntary cancellation, (b) agree to satisfy the
 product specific data requirements imposed by this Notice or (c)
 request a data vaiver(s).

     A discussion of how to respond if you choose the Voluntary
 Cancellation option is presented below.  A discussion of the
. various options available for satisfying the product specific data
 requirements of this Notice is contained in Section III-C.  A
 discussion of options relating to requests for data waivers is
 contained In Section XII-D.

     There are two forms that accompany this Notice of which,
 depending upon your response, one or both must be used in your
 response to the Agency.  These forms are the Data-Call-in, Response
 Fora,  and the Requirements Status and Registrant's Response form.
 Attachment B and Attachment C.  The Data Call-In Response Form must
 be submitted as part of every response, to, this Notice.  Xn
 addition,  one copy of the Requirements Status and Registrant's
 Response Form  must be submitted for each product listed on the
 Data Call-in Response Form unless the voluntary cancellation option
 is selected or unless the product is identical to another (refer to
 the instructions for completing the pafra gall-In Response Form in
 Attachment B).  Please note that the company's authorized
 representative is required to sign the first page of the Data Call-
 in Response Form and Requirements Status and Registrant's Response
 Fjyas (if this form is required)  and initial any subsequent pages.
 The forms contain separate detailed instructions on the response
 options.   Do not alter the printed material.  Xf you have questions
 or need assistance in preparing your response, call or write the
 contact person(s)  identified in Attachment A.

     1.  Voluntary cancellation - You nay avoid the requirements of
 this Notice by requesting voluntary cancellation of your product(s}
 containing the active ingredient that is the subject of this
 Notice.  Xf you wish to voluntarily cancel your product, you must
 submit  a completed Data Call-in Response Form, indicating your
 election of this option.   Voluntary cancellation is item number 5
 on the  Data  call-in Response Form.  Xf you choose this option, this
 is the  only form that you are required to complete.

     Xf  you choose to voluntarily cancel your product, further sale
 and distribution of your product after the effective date of
 cancellation must be in accordance  with the Existing Stocks
 provisions of this Notice which  are contained in Section XV-c.

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     2. Satisfying the Product: Specific Data Requirements of thip
 Notice.  There are various options available to satisfy the product
 specific data requirements of this Notice.  These options are
 discussed in Section III-C of this Notice and coaprise options 1
 through 6 on the Requirements Status and Registrant's Response Porn
 and item numbers 7 a and 7b on. the Data Call-In Response Porn.
 Deletion of a use(s) and the low volume/minor use option are not
 valid options for fulfilling product specific data requirenents.

     3. Request for Product Specific Data Waivers.  Waivers for
 product specific data are discussed in Section III-D of this Notice
 and are covered by option 7 on the Requirements Status and
 Reqistrant's Response Pqrn.  If you choose this option, you oust
 submit both forms as well as any other information/data pertaining
 to the option chosen to address the data requirement.

 III-C  SATISFYING THE DATA REQUIREMENTS Of THIS NOTICE

      If you acknowledge on the Data Call-in Response Form that you
 agree to satisfy the product specific data requirements (i.e. you
 select option 7a or 7b), then you must select one of the six
 options on the Recfulrenents Status and Registrant's Response Form
 related to data production for each data requirement.  Your option
 selection should be entered under item number 9, "Registrant
 Response."  The six options related to data production are the
 first six options discussed under item 9 in the instructions for
 completing the Requirements Status and Registrant's Response Form.
 These six options are listed immediately below with information in
 parentheses  to guide registrants to additional instructions
 provided in  this Section.   The options are:

      (1)   I  will generate and submit data within the specified
           timeframe (Developing Data)
      (2)   I  have entered, into an agreement with one or more
           registrants to develop data jointly (Cost Sharing)
      (3)   I  have made offers to cost-share (Offers to Cost Share)
      (4)   I  am submitting an existing study that has not been
           submitted previously to the Agency by anyone (Submitting
           an Existing Study)
      (5)   I  am submitting or citing data to upgrade a study
           classified by  EPA as partially acceptable and upgradeable
           (Upgrading a Study)
      (6}   I  am citing an existing study that EPA has  classified as
           acceptable or  an existing study that has been submitted
           but not reviewed by the Agency (Citing an Existing Study)

     Option  l,.  Develflpinp Data —  If you choose to develop the
required data it  must be in conformance with Agency, deadlines and
     other Agency requirements as referenced herein and in the
  tachments.  All data generated and submitted must comply with the
Good Laboratory Practice (GLP)  rule (40 CFR Part 160),  be conducted
according  to  the  Pesticide Assessment Guidelines (PAG),  and be in
conformance with  the  requirements of PR Notice 86-5.

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     The tine frames in the Requirements Status and Registrant's
 Response Fopn are the time frames that the Agency is  allowing for
 the submission of completed study reports.  The noted deadlines  run
 from the date of the receipt of this Notice by the registrant.   If
 the data are not submitted by the deadline, each registrant is
 subject to receipt of a Notice of Intent to Suspend the  affected
 registration(s).

     If you cannot submit the data/reports to the Agency  in the time
 required by this Notice and intend to seek additional time to meet
 the requirements (s), you must submit a request to the Agency which
 includes: (1)  a detailed description of the expected  difficulty  and
 (2) a proposed schedule including alternative dates for  meeting
 such requirements on a step-by-step basis.  You must  explain any
 technical or laboratory difficulties and provide documentation from
 the laboratory performing the testing.  While EPA is  considering
• your request,  the original deadline remains.  The Agency will
 respond to your request in writing.  If EPA does not  grant your
 request, the original deadline remains.  Normally, extensions can
 be requested only in cases of extraordinary testing problems beyond
 the expectation or control of the registrant.  Extensions  will not
 be given in submitting the 90-day responses.  Extensions will not
 be considered if the request for extension is not made in  a timely
 fashion; in no event shall an extension request be considered if it
 is submitted at or after the lapse of the subject deadline.

      Option 2.  Agree to Share in Coat to Develop Data —Registrants
 may only choose this option for acute toxicity data and  certain
 efficacy data  au& only if EPA has indicated in the attached data
 tattles that your product and at least one other product  are similar
 for purposes of depending on the same data.  If this  is  the case,
 data may be generated for just one of-the products in the  group.
 Tttfe registration n^l^tT of the product for which data will be
 submitted must be noted in the agreement to cost share by  the
 registrant selecting this option.   If you choose to enter  into an
 agreement to share in the cost of producing the required data but
 will not be submitting the data yourself, you must provide the name
 of the registrant Jrtio will be submitting the data.  You  must also
 provide EPA with documentary evidence that an agreement  has been
 formed.   Such  evidence may be your letter offering to join in an
 agreement and  the other registrant's acceptance of your  offer, or a
 written statement by the parties that an agreement exists.   The
 agreement to produce the data need not specify all of the  terms  of
 the final arrangement between the parties or the mechanism to
 resolve  the terms.   Section 3(c)(2)(B)  provides that  if  the parties
 cannot resolve  the terms of the agreement they may resolve their
 differences through binding arbitration.
    Option 3. Offer to Share in the Cost of Data Development  —
This option only applies to acute toxicity and certain efficacy
data as described in option 2 above.  If you have made an offer to
pay in an attempt to enter into an agreement or amend an existing
agreement to meet the requirements of this Notice and have been

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 unsuccessful, you nay request EPA (by selecting this option) to
 exercise its discretion not to suspend your registration(s),
 although you do not comply with the data submission requirements of
 this Notice.  EPA has determined that as a general  policy,  absent
 other relevant considerations, it will not suspend  the registration
 of a product of a registrant who has in good faith  sought and
 continues to seek to enter into a joint data development/cost
 sharing program, but the other registrant(s)  developing the data
 has refused to accept your offer.  To qualify for this option, you
 must submit documentation to the Agency proving that you have made
 an offer to another registrant (who has an obligation to submit
 data) to share in the burden of developing that data.   You  must
 also submit to the Agency a completed EPA Form 8570-32,
 Certification of Offer to Cost Share in the Development of  Data,
 Attachment 6.  In addition, you must demonstrate that the other
 registrant to whom the offer vas made has not accepted your offer
 td enter into a costsharing agreement by including  a copy of your
 offer.and proof of the other registrant's receipt of that offer
 (such as a certified mail receipt).   Your offer must,  in addition
 to anything else,  offer to share in the burden of producing the.
 data upon terms to be agreed or failing agreement to be bound by
 binding arbitration as provided by FIFRA section 3(c)(2)(B)(iii)
 and must not qualify this offer.   The other registrant must also
 inform EPA of its election of an option to develop  and submit the
 data required by this Notice by submitting a Data Call-In Response
 Form and a fteouirenents Status and Registrant's Response Form
 committing to develop and submit the data required  by this  Notice.

     Zn  order for you to avoid suspension under this option, you may
 not -withdraw your offer to share in the burdens of  developing the
 data.   In addition,  the other registrant must fulfill its
 commitment to develop and submit the data as required by this
 Notice.   If the other registrant fails to develop the data  or for
 some other reason is subject to suspension,  your registration as
 well as that of the other registrant will normally  be subject to
 initiation of suspension proceedings,  unless you commit to  submit,
 and do  submit the required data in the specified time frame.  In
 such cases,  the Agency generally will not grant a time extension
 for submitting the data.

     Option 4.  Submitting an Existing Study •»• If you choose to
 submit  an existing study in response to this Notice, you must
 determine that the study satisfies the requirements imposed by this
 Notice.   You may only submit a study that has not been previously
 submitted to the Agency or previously cited by anyone.   Existing
 studies  are  studies which predate issuance of this  Notice.  Do not
 use  this option if you are submitting data to upgrade  a  study. (See
 Option 5).

     You  should be  aware that if the  Agency determines  that  the
 study is not acceptable,  the Agency  will  require you to  comply with
this Notice, normally without an extension of the required  date of
submission.  The Agency may determine at  any time that a study is
not valid and needs to  be repeated.

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                                 8

    To meet the requirements of the DCI Notice  for submitting an
existing study, all of the follovingthree criteria must be clearly
net;

    a. You aust certify at the tine that the existing study is
    submitted that the raw. data and specimens from the study are
    available for audit and review  and you aust identify where they
    are available.  This aust be done in accordance with
    the requirements of the Good Laboratory Practice  (GLP)
    regulation, 40 CFR Part 160. As stated in 40 CFR 160.3(j)
    * 'Crjaw data1 means any laboratory worksheets, records,
 •   memoranda, notes, or exact copies thereof,  that are the result
    of original observations and activities of  a study and are
 ~  necessary for the reconstruction and evaluation of the report
 _.  of that study.  In the event that exact transcripts of raw data
 ''. -have been prepared (e.g.,  tapes which have  been transcribed
    verbatim, dated, and verified accurate by signature), the exact
    copy or exact transcript may be substituted for the original
    source as raw data.  'Raw data1  may include photographs,
    microfilm or microfiche copies,  computer printouts, magnetic
    media, including dictated observations, and recorded data from
    automated instruments."  The term "specimens", according to 40
    CFR 160.3(k), means "any material derived from a test system
    for examination or analysis."

    b. Health and safety studies completed after Kay 1984 must also
    contain all GLP-required quality assurance  and quality control
    information,  pursuant to the requirements of 40 CFR Part 160.
    Registrants must also certify at the time of submitting the
    existing study that such GLP information is available for post-
    May 1984  studies by including an appropriate .statement on or
 ':  attached to the study signed by an authorized official or
  •  representative of the registrant.

    c. You must certify that each study fulfills the acceptance
    criteria for the Guideline relevant to the  study provided in
    the FIFRA Accelerated Reregistration Phase  3 Technical Guidance
    and that  the study has been conducted according to the
  ;  Pesticide Assessment Guidelines (PAG) or meets the purpose of
    the PAG (both available from KTZS).  A study not conducted
    according to the PAG may be submitted to the Agency for
    consideration if the registrant believes that the study clearly
    meets  the purpose of the PAG.   The registrant is referred to 40
    CFR 158.70 which states the Agency's policy regarding
    acceptable protocols.  If you wish to submit the study, you
    must,  in  addition to certifying that the purposes of the PAG
    are  net by the study,  clearly articulate the rationale why you
    believe the study meets the purpose of the  PAG, including
    copies of any supporting information or data.  It has been the
    Agency's  experience that studies completed  prior to January
    1970 rarely satisfied .the  purpose of the PAG and that necessary
    raw  data  are  usually not available for such studies.

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     If you submit an existing study,  you Bust certify that the
 study meets all requirements of the criteria outlined above.

     Zf you know of a study pertaining to any requirement in this
 Kotice which does not meet the criteria outlined above but does
 contain factual information regarding unreasonable adverse effects,
 you aust notify the Agency of such a  study.  Zf such  study is in
 the Agency's files, you need only cite it along with the
 notification. Zf not in the Agency's  files, you Bust submit a
 summary and copies as required by PR  Notice 86-5.


     Option S. Upgrading astudy — Zf a study has been classified
 as partially acceptable and upgradeable,  you may submit data to
 upgrade that study.  The Agency will  review the data submitted and
 determine if the requirement is satisfied.  Zf the Agency decides
 the requirement is not satisfied,  you aay still be required to
 submit new data normally without any  time extension.  Deficient,
 but upgradeable studies will normally be classified as
 supplemental.  However, it is important to note that not all
 studies classified as supplemental are upgradeable.  Zf you have
 questions regarding the classification of a study or whether a
 study aay be upgraded,  call or write  the contact person listed in
 (Attachment A*  Zf you submit data to  upgrade an existing study you
 aust satisfy or supply information to correct all deficiencies in
 the study identified by EPA.  You must provide a clearly
 articulated rationale of how the deficiencies have been remedied or
 corrected and why the study should be rated as acceptable to EPA.
 Your submission must also specify the KRID number(s) of the study
 which you are attempting to upgrade and must be in conformance with
 PR Notice 86-5.

     Do not submit additional data for the purpose of upgrading a
 study classified as unacceptable and  determined by the Agency as
 not capable of being upgraded.

     This  option should also be used to cite data that has been
 previously submitted to upgrade a  study,  but has not yet been
 reviewed  by the Agency.  You must  provide the MRID number of the
 data submission as well as the MRID number of the study being
 upgraded.

     The criteria for submitting an existing study, as specified in
 Option 4  above,  apply to all data  submissions intended to upgrade
 studies.  Additionally  your submission of data intended to upgrade
 studies aust be accompanied by a certification that you comply with
 each of those criteria  as veil  as  a certification regarding
protocol  compliance with Agency requirements.
    Option 6. citing Existing Studies — Zf you choose to cite a
      that has been previously submitted to EPA, that study must
have been previously classified by EPA as acceptable or it must be
a study which has not yet been reviewed by the Agency.  Acceptable

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                                 10

 toxicology studies generally vill  have been classified as "core-
 guideline" or "core minimum."  For all other disciplines the
 classification vould be "acceptable."  With respect to any studies
 for which you wish to select this  option you must provide the MR ID
 number of the study you are citing and, if the study has been
 reviewed by the Agency,  you Bust provide the Agency's
 classification of the study.

     Zf you are citing a study of which you are not the original
 data submitter,  you must submit a  completed copy of EPA Form
 8570-31, Certification with Respect to Data Compensation
 Requirements.

   *  Registrants who select  one  of  the above 6 options must meet all
 of the requirements described in the instructions for completing
 the pata^ Call-in Response Form  and .the Requirements Status and
 Registrant's Response Form,  as  appropriate.

 ZZZ-D  REQUESTS  FOR DATA WAIVERS

     Zf you request a waiver for product specific data because you
 believe it is  inappropriate,  you oust attach a complete
 justification  for the request,  including technical reasons, data
 and references to relevant  EPA  regulations, guidelines or policies.
 (Note:  any supplemental  data  must  be submitted in the format
 required by PR Notice 86-5).  This will be the only opportunity to
 .state the reasons or provide  information in support of your
 request.   Zf the Agency  approves your waiver request, you will not
 be required to supply the data  pursuant to section 3(c)(2)(B) of
 FZFRA.   Zf the Agency denies  your  waiver request, you must choose
 an option for  meeting the data  requirements of this Notice within
 30 days of the receipt of the Agency's decision.  You must indicate
 and submit the option chosen  on the Requirements status and
 Registrant's Response Form.   Product specific data requirements for
 product chemistry,  acute toxicity  and efficacy (where appropriate)
 are required for all  products and  the Agency will grant a waiver
 only under extraordinary circumstances.  You should also be aware
 that submitting  a waiver request will &££ automatically extend the
 due date  for the study in question.  Waiver requests submitted
 without adequate supporting rationale will be denied and the
 original due date will remain in force.

 ZV.   CONSEQUENCES OF  FAILURE  TO COMPLY WITH THIS NOTICE

 ZV-A NOTICE OF INTENT TO  SUSPEND

    The Agency may  issue  a Notice  of Zntent to Suspend products
 subject to this Notice due to failure by a registrant to comply
with the requirements  of  this Pata  Call-in Notice, pursuant to
 FZFRA section  3(c)(2)(B).  Events which may be the basis for
 issuance of a Notice  of  Zntent  to Suspend include, but are not
 limited to, the "following:

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                                 11

     1.  Failure to respond as required by this Notice within 90
     days of your receipt of this Notice.

     2.  Failure to submit on the required schedule an acceptable
     proposed or final protocol if such is required to be submitted
     to the Agency for review.

     3.  Failure to submit on the required schedule an adequate
     progress report on a study if required by this Notice.

     4.  Failure to submit on the required schedule acceptable
     data as required by this Notice.

     5.  Failure to take a required action or submit adequate
    .-• information pertaining to any option  chosen to address the data
     requirements (e.g., any required  action or information
     pertaining to submission or citation  of existing studies or
     offers,  arrangements, or arbitration  on the sharing of costs or
     the formation of Task Forces,  failure to comply with the terms
     of an agreement or arbitration concerning joint data
     development or failure to comply  with any terms of a data
     waiver).

     6.  Failure to submit supportable certifications as to the
     conditions of submitted studies,  as required by Section  III-C
     of this Notice.

     7. Withdrawal of an offer to share in the cost of developing
   .  required  data.

     8. Failure of the registrant to whom  you have tendered an offer
     to share  in the cost of developing data and provided proof of
     the registrant's receipt of such  offer either to:

     a.  Inform EPA of intent to develop and submit the data
     required by this Notice on a  Data Call-In Response Form and a
     Requirements Status and Registrant's Response Form;

     b.  Fulfill the commitment to  develop and submit the data as
     required by this Notice;  or

   • c.  Otherwise take appropriate  steps to meet the requirements
    stated in this  Notice,  unless  you commit to submit and do
    submit the required data in the specified time frame.

    9.   Failure to  take any required  or appropriate steps, not
    mentioned above,  at any time following the issuance of this
    Notice.

IV-B.  BASIS  FOR PETESMIMATIQW THAT SUBMITTED STUDY IS
    The Agency may determine that a study (even if submitted within
the required time) is unacceptable and constitutes a basis for

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                                 12

 issuance of a Notice of Intent to Suspend.  The grounds for
 suspension include, but are not limited to, failure to meet any of
 the following:

     1.   EPA requirements specified in the Data Call-In Notice or
     other documents incorporated by reference (including, as
     applicable, EPA Pesticide Assessment Guidelines, Data Reporting
     Guidelines, and GeneTox Health Effects Test Guidelines)
     regarding the design, conduct, and reporting of required
     studies.  Such requirements include, but are not limited to,
    . those relating to test .material, test procedures, selection of
     species, number of animals, sex and distribution of animals,
 . '   dose and effect levels to be tested or attained, duration of
 .. ,  test,  and, as applicable, Good Laboratory Practices.
    2.  EPA requirements regarding the submission of protocols
    applicable), including the incorporation of "any changes
    required by the Agency following review.
                                                               (if
    3.  EPA requirements regarding the reporting of data, including
    the manner of reporting, the completeness of results, and the
    adequacy of any required supporting (or raw) data, including,
    but not limited to, requirements referenced or included in this
    Notice or contained in PR 86-5.  All studies must be submitted
    in the form of a final report; a preliminary report will not be
    considered to fulfill the submission requirement.
IV-C  EXISTING STOCKS OF SUSPENDED OR
                                                PRODUCTS
  _  EPA has statutory authority to permit continued sale,
distribution and use of existing stocks of a pesticide product
which has been suspended or ^cancelled* if doing -so would be
consistent with the purposes of the Act.

    The Agency has determined that such disposition by registrants
of existing stocks for a suspended registration when a section
3(c)(2)(B) data request is outstanding would generally not be
consistent with the Act's purposes.  Accordingly, the Agency
anticipates granting registrants permission to sell, distribute, or
use existing stocks of suspended product (s) only in exceptional
circumstances.  If you believe such disposition of existing stocks
of your product (s) which may be suspended for failure to comply
with this Notice should be permitted, you have the burden of
clearly demonstrating to EPA that granting such permission would be
consistent with the Act. You must also explain why an "existing
stocks" provision is necessary, including a statement of the
quantity of existing stocks and your estimate of the time required
for their sale, distribution, and use.  Unless you meet this burden
the Agency will not consider any request pertaining to the
continued sale, distribution, or use of your existing stocks after
suspension.

    If you request a voluntary cancellation of your product (s) as a
response to this Notice and your product is in full compliance with

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                                  13

 all Agency requirements, you will have, under most circumstances,
 one year from the date your 90 day response to this Notice is due,
 to sell, distribute, or use existing stocks.  Normally,  the Agency
 vill allow persons other than the registrant such as independent
 distributors, retailers and end users to sell, distribute or use
 such existing stocks until the stocks are exhausted.  Any sale,
 distribution or use of stocks of voluntarily cancelled products
 containing an active ingredient for which the Agency has particular
 risk concerns vill be determined on a case-by-case basis.

     Requests for voluntary .cancellation received after the 90 day
 response period required by .this Notice vill not result  in the
 Agency granting any additional time to sell, distribute, or use
 existing stocks beyond a year from the date the 90 day response  was
 due unless you demonstrate to the Agency that you are in full
 compliance with all Agency requirements, including the requirements
 of this Notice.  For example,  if you decide to voluntarily cancel
 your registration six months before a 3 year study is scheduled  to
 be submitted, all progress reports and other information necessary
 to establish that .you have been conducting the study in  an
 acceptable and good faith manner must have been submitted to the
 Agency,  before EPA vill consider granting an existing stocks
 provision.
 SECTION V.   REGISTRANTS'  OBLIGATION TO REPORT
             UNREASONABLE ADVERSE EFFECT^

     Registrants are reminded that FIFRA section 6(a)(2)  states that
 if at any time after a pesticide is registered a registrant has
 additional factual  information regarding unreasonable adverse
 effects  on the environment by the pesticide,  the registrant shall
 submit the information to the Agency.   Registrants must  notify the
 Agency of any  factual information they have,  from whatever source,
 including but  not limited to interim or preliminary results of
 studies,  regarding  unreasonable adverse effects on man or the
 environment.   This  requirement continues as long as the  products
 are registered by the Agency.
SECTION VI.  INQUIRIES AND RESPONSES TO THIS  NOTICE

    If you have any questions regarding the requirements  and
procedures established by  this Notice, call the contact person(s)
listed in Attachment A, the Data Call-In chemical Status  Sheet.

    All responses to this  Notice (other than  voluntary cancellation
requests) must include a completed Data Call-in Response  Fern and a
completed RequirementsStatus and Registrant's Response Form
 Attachment B and Attachment C) and any other documents required by
  is Notice, and should be submitted to the contact person(s)
identified in Attachment A. If the voluntary  cancellation option is
chosen, only the pata Call-InResponse Form need be submitted.

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    The Office of Compliance Monitoring (OCM)  of the Office of
Pesticides and Toxic Substances (OPTS),  EPA, will be monitoring the
data being generated in response to this Notice.
                             Sincerely yours,
    A  -
    B  •
    C  -
    D  -

    E -
    F  •
    6  -
                    Daniel M. Barolo, Director
                  . ' Special Review and
                      Reregistration Division

               Attachments

Data Call-in Chemical Status Sheet
Data Call-in Response Form
Requirements Status and Registrant's Response Forjn
EPA Grouping of End-Use Products for Meeting Acute
Toxicology Data Requirements for Rereoistratign
EPA Acpepta^ce Criteria
List of Registrants Receiving This Notice
Cost Share and Data Compensation Forms.  and Product
Specific Data Report Form

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    ATTACHMENT A




CHEMICAL STATUS SHEET

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                                 ATTACHMENT A

              N. locustae: DATA CALL-IN CHEMICAL STATUS SHEET

INTRODUCTION

       You have been sent this Data Call-In Notice because you have products containing N.
locustae.

       This attachment, the Data Call-in Chemical Status Sheet, contains a point of contact for
inquiries.  This attachment is to be used in conjunction with (1) the Data Call-In Notice. (2)
Attachment B, the Data Call-In Response Form. (3) Attachment C, the Requirement Status and
Registrant's Response Form for product specific data, (4) Attachment D, EPA Grouping of End-
Use Products for  Meeting Acute  Toxicology  Data Requirements for Reregistration. (5)
Attachment E, EPA Acceptance Criteria. (6) Attachment F, List of All Registrantfsl sent this
Data Call-In Notice, and (7) Attachment G, the Cost Share and Data Compensation Forms, for
product specific data,  and ProducUSpecific Data Report Form for use in replying to this name
of chemical Data Call-In. Instructions and guidance accompany each form.

DATA REQUIRED BY THIS NOTICE

       The additional data requirements needed to complete the database for name of chemical
are listed in the Requirements Status and Registrant's Response Form.  Attachment C.

       The Agency has concluded that product specific data are needed for name of chemical.
The required additional data are listed in  Attachment C.

       Depending on the results of the studies required in this Notice, additional testing may be
required.

INQUIRIES AND RESPONSES TO THIS NOTICE

       If you have any questions- regarding the product specific data requirements  and
procedures established by this  Notice, please contact Phil Button at  (703) 305-7690.  All
responses to this Notice should be submitted to:

                   Document Processing Desk (RED/RD/PM-18)
                   Office of Pesticide Programs
                   U.S. Environmental Protection Agency
                   401 M Street S.W.
                   Washington, D.C. 20460

                   RE: N. locustae

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      If you  have any questions regarding the generic  data requirements and procedures
established by this Notice, please contact Sue Rathman at (703) 308-8069. All responses to this
Notice should be submitted to:

                    Chemical Review Manager Sue Rathman
                    Accelerated Reregistration Branch (H7508W)
                    Special Review and Reregistration Division
                    Office of Pesticide Programs
                    U.S. Environmental Protection Agency
                    401 M Street S.W.
                    Washington, D.C. 20460

                    RE: N.  locustae

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                  ATTACHMENT B

PRODUCT SPECIFIC DATA CALL-IN RESPONSE FORMS (Form A)
                 PLUS INSTRUCTIONS

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                    SPECIFIC INSTRUCTIONS FOR
                  THE  DATA CALL-IN RESPONSE FORM
     This form is designed to be used to respond to call-ins for
generic and product specific data for the purpose of
reregistering pesticides under the Federal Insecticide Fungicide
and Rodenticide Act.  Fill out this form each time you are
responding to a data call-in for which EPA has sent you the form
entitled "Requirements Status and Registrant's Response.11

     Items 1 -4 will have been preprinted on the form. Items 5
through 7 must be completed by the registrant as appropriate.
Items 8 through 11 must be completed by the registrant before
submitting a response to the Agency.

     Public reporting burden for this collection of information
is estimated to average 15 minutes per response, including time
for reviewing instructions, searching existing data sources,
gathering and maintaining the data needed, and completing and
reviewing the collection of information.  Send comments regarding
the burden estimate or any other aspect of this collection of
information, including suggesting for reducing this burden, to
Chief, Information Policy Branch, PM-223, U.S. Environmental
Protection Agency, 401 M St., S.W., Washington, D.C. 20460; and
to the Office of Management and Budget, Paperwork Reduction
Project 2070-0107, Washington, D.C. 20503.
INSTRUCTIONS

Item 1.   This item identifies your company name, number and
          address.

Item 2.   This item identifies the case number, case name, EPA
          chemical number and chemical name.

Item 3.   This item identifies the date and type of data call-in.

Item 4.   This item identifies the EPA product registrations
          relevant to the data call-in.  Please note that you are
          also responsible for informing the Agency of your
          response regarding any product that you believe may be
          covered by this data call-in but that is not listed by
          the Agency in Item 4.  You must bring any such apparent
          omission to the Agency's attention within the period
          required for submission of this response form.

Item 5.   Check this item for each product registration you wish
          to cancel voluntarily.  If a registration number is
          listed for a product for which you previously requested
          voluntary cancellation, indicate in  Item 5 the date of
          that request.  You do not need to complete any item on

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          the Requirements Status andRegistrant's Response  Form
          for any product that is voluntarily cancelled.

 Item  6a.  Check this item if this data call-in is for generic
          data as indicated in Item 3 and if you are eligible for
          a Generic Data Exemption for the chemical listed in
          Item 2 and used in the subject product.  By electing
          this exemption, you agree to the terms and conditions
          of a Generic Data Exemption as explained in the Data
          Call-in Notice.

          If you are eligible for or claim a Generic Data
          Exemption, enter the EPA registration Number of each
          registered source of that active ingredient that you
          use in your product.

          Typically, if you purchase an EPA-registered product
          from one or more other producers (who, with respect to
          the incorporated product, are in compliance with this
          and any other outstanding Data Call-In Notice), and
          incorporate that product into all your products, you
          nay complete this item for all products listed on this
          form.  If, however, you produce the active ingredient
          yourself, or use any unregistered product (regardless
          of the fact that some of your sources are registered),
          you may not claim a Generic Data Exemption and you may
          not select this item.

Item  6b.  Check this Item if the data call-in is a generic data
          call-in as indicated in Item 3 and if you are agreeing
          to satisfy the generic data requirements of this data
          call-in.  Attach the Requirements Status and.
          Registrant's Response Form that indicates how you will
          satisfy those requirements.

Item  7a.  Check this item if this call-in is a data call-in as
          indicated in Item 3 for a manufacturing use product
          (MUP), and if your product is a manufacturing use
          product for which you agree to supply product-specific
          data.  Attach the Requirements Status and Registrants*
          Response Form that indicates how you will satisfy those
          requirements.

Item 7b.  Check this item if this call-in is a data call-in  for
          an end use product (EUP) as indicated in Item 3 and  if
          your product is a end use product for which you agree
          to supply product-specific data.  Attach the
          Requirements Status andRegistrant's Response Form that
          indicates how you will satisfy those requirements.

Item 8.   This certification statement must be signed by an
          authorized representative of your company and the

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Item 9.

Item 10.
person signing must include his/her title.   Additional
pages used in your response must be initialled and
dated in the space provided for the certification.

Enter the date of signature.

Enter the name of the person EPA should contact with
questions regarding your response.
Item 11.  Enter the phone number of your company contact.

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                       ATTACHMENT C

PRODUCT SPECIFIC REQUIREMENT STATUS AND REGISTRANT'S RESPONSE
                 (FORMS B) PLUS INSTRUCTIONS
                            AND
                        PR NOTICE 86-5

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               SPECIFIC INSTRUCTIONS  FOR COMPLETING
     THE REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE FORK


                      Product specific Data


     This form is designed to be used for registrants to respond
to call-ins for generic and product-specific data as part of
EPA*s reregistration program under the Federal Insecticide
Fungicide and Rodenticide Act.   Although the  form is the same
for both product and generic data, instructions for completing
the forms differ slightly.  Specifically, options for satisfying
product specific data requirements do not include (1) deletion of
uses or (2) request for a low volume/minor use waiver.  These
instructions are for completion of product specifie data
requirements.

     EPA has developed this form individually for each data call-
in addressed to each registrant, and has preprinted this form
with a number of items.  DO NOT use this form for any other
active ingredient.

     Items 1 through 8 (inclusive) will have been preprinted on
the form.   You must complete all other items on this form by
typing or printing legibly.

     Public reporting burden for this collection of information
is estimated to average 30 minutes per response, including time
for reviewing instructions, searching existing data sources,
gathering and maintaining the data needed, and completing and
reviewing the collection of information.  Send comments regarding
the burden estimate or any other aspect of this collection of
information, including suggestions for reducing this burden, to
Chief, Information Policy Branch, PM-223, U.S. Environmental
Protection Agency, 401 M St., S.W., Washington, D.C.  20460; and
to the Office of Management and Budget, Paperwork Reduction
Project 2070-0107, Washington, D.C.  20503.

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     INSTRUCTIONS FOR COMPLETING THE "REQUIREMENTS STATUS AND
      REGISTRANT'S RESPONSE" FORM FOR PRODUCT  SPECIFIC DATA


Itorn 1-3  completed by EPA.   Note the unique identifier number
          assigned by EPA in Item 3.   This number must be used  in
          the transmittal document for any data submissions  in
          response to this Data Call-In Notice.

Item 4.   The guideline reference numbers  of studies required to
          support the product's continued  registration are
          identified.   These guidelines, in addition to the
          requirements specified in the Notice, govern the
          conduct of the required studies. Note  that series 61
          and 62 in product  chemistry are  nov  listed under 40 CFR
          158.155 through 158.180, Subpart C.

Item 5*   The study title associated  with  the  guideline reference
          number is identified*

Item 6.   The use pattern(s) of the pesticide  associated  with the
          product specific requirements is (are)  identified. For
          most product specific data  requirements,  all use
          patterns are covered by the data requirements.  In the
          case of efficacy data, the  required  studies only per-
          tain to products which have the  use  sites and/or pests
          indicated.

Item 7.   The substance to be tested  is identified by EPA.   For
          product specific data, the  product as  formulated for
          sale and distribution is the test substance, except in
          rare cases.

Item 8.   The due date for submission of each  study is
          identified.   It is normally based on 8  months  after
          issuance of the Reregistration Eligibility Document
          unless EPA determines that  a longer  time period is
          necessary.

Item 9.   Enter only one of  the following  response codes for each
          data requirement to show how you intend to comply  with
          the data requirements listed in  this table.   Fuller
          descriptions of each option are  contained in  the Data
          Call-in Notice.

     1.   I will generate and submit  data  by the specified due
          date (Developing Data).  By indicating that I have
          chosen this option, I certify that I will comply with
          all the requirements pertaining  to the conditions  for
          submittal of this  study as  outlined  in the Data Call-In
          Notice.

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I have entered into an agreement with one or more
registrants to develop data jointly (Cost Sharing).  I
an submitting a copy of this agreement and a completed
•Certification With Respect To Data Compensation
Requirements*1 form.  I understand that this option is
available only for acute toxicity or certain efficacy
data only if EPA indicates in an attachment to this
Notice that my product is similar enough to another
product to qualify for this option.  I certify that
another party in the agreement is committing to submit
or provide the requirement data; if the required study
is not submitted on time, my product may be subject to
suspension.

I have made offers to share in the cost to develop data
(Offers to Cost Share).  Z understand that this option
is available only for acute toxicity or certain
efficacy data and only if EPA indicates in an
attachment to this Data Call-In Notice that my product
is similar enough to another product to qualify for
this option.  I am submitting evidence that I have make
an offer to another registrant (who has an obligation
to submit data) to share in the cost of that data.  I
am also submitting a completed "Certification of Offer
to Cost Share in the Development Data" form.   I am
including a copy of my offer and proof of the other
registrant's receipt of that offer.  I am identifying
the party which is committing to submit or provide the
required data; if the required study is not submitted
on time, my product may be subject to suspension.  I
understand that other terms under Option 3 in the Data
Call-In Notice (Section III-C.l.) apply as well.

By the specified due date, I will submit an existing
study that has not been submitted previously to the
Agency by anyone (Submitting an Existing Study).  I.
certify that this study will meet all the requirements
for submittal of existing data outlined in Option 4 in
the Data Call-In Notice (Section III-C.l.) and will
meet the attached acceptance criteria (for acute
toxicity and product chemistry data).   I will attach
the needed supporting information along with this
response.  I also certify that I have determined that
this study will fill the data requirement for which I
have indicated this choice.

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By the specified due date,  Z vill «ubait or cite data
to upgrade a study classified by the Agency as
partially acceptable and upgradable (Upgrading a
Study).  X vill submit evidence of the Agency's reviev
indicating that the study nay be upgraded and what
information is required to do so.  X vill provide the
MRID or Accession number of the study at the due date.
X understand that the conditions for this option
outlined Option 5 in the Data Call-In Notice (Section.
XXX-C.l.) apply.

By the specified due date,  X vill cite an existing
study that the Agency has classified as acceptable or
an existing study that has been submitted but not
revieved by the Agency (Citing an Existing Study).  Xf
X am citing another registrant's study, X understand
that this option is available only for acute toxicity
or certain efficacy data and only if the cited study
vas conducted on my product, an identical product or a
product which EPA has "grouped" with one or more other
products for purposes of depending on the same data.  X
may also choose this option if X am citing my own data.
Xn either case, X vill provide the MRID or Accession
number(s) for the cited data on a "Product Specific
Data Report" form or in a similar format.  Xf X cite
another registrant's data,  X vill submit a completed
"Certification With Respect To Data Compensation
Requirements" form.

X request a vaiver for this study because it is
inappropriate for my product (Waiver Request).  X am
attaching a complete justification for this request,
including technical reasons, data and references to
relevant EPA regulations, guidelines or policies.
[Note:  any supplemental data must be submitted in the
format required by P.R. Notice 86-5].  X understand
that this is my only opportunity to state the reasons
or provide information in support of my request.  Xf
the Agency approves my vaiver request, X vill not be
required to supply the data pursuant to Section
3(c)(2)(B) of FXFRA.  Xf the Agency denies my vaiver
request, X must choose a method of meeting the data
requirements of this Notice by the due date stated by
this Notice.  Xn this case, X must, within 30 days of
my receipt of the Agency's written decision, submit a
revised "Requirements Status and Registrant's Response'1
Form indicating the option chosen.  X also understand
that the deadline for submission of data as specified
by the original data call-in notice vill not change.

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Items 10*13    Self-explanatory.

NOTE;     you nay provide additional information that does not
          fit on this form in a signed letter that accompanies
          this form.  For example,  you may wish to report that
          your product has already been transferred to antoher
          company or that you have already voluntarily cancelled
          this product.  For these cases,  please supply all
          relevant details so that EPA can ensure that its
          records are correct.

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          UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

                      WASHINGTON, D.C. 20460
                                                   11 29
                        PR NOTICE 86-5
       OFFICE Of
PESTICIBC* AND TOXIC SUBSTANCES
        NOTICE TO PRODUCERS, FORMULATORS, DISTRIBUTORS
                       AND REGISTRANTS

Attention:  Persons responsible for Federal registration of
            pesticides.

Subject:    Standard format for data submitted under the Federal
            Insecticide, Fungicide, and Rodenticide Act (FIFRA)
            and certain provisions of the Federal Food, Drug,
            and Cosmetic Act (FFDCA).

I.   Purpose

     To require data to be submitted to the Environmental
Protection Agency (EPA) in a standard format*  This Notice also
provides additional guidance about, and illustrations of, the
required formats.

II.  Applicability

     This PR Notice applies to all data that are submitted to EPA
to satisfy data requirements for granting or maintaining pesticide
registrations, experimental use permits, tolerances, and related
approvals under certain provisions of FIFRA and FFDCA.  These
data are defined in FIFRA SlO(dHl).  This Notice does not apply
to commercial, financial, or production information, which are,
and must continue to be, submitted differently under separate
cover.

III. Effective Date

     This notice is effective on November 1, 1986.  Data formatted
according to this notice may be submitted prior to the effective
date.  As of the effective date, submitted data packages that do
not conform to these requirements may be returned to the submitter
for necessary revision.

IV.  Background

     On September 26, 1984, EPA published proposed regulations
in the Federal Register (49 FR 37956) which include Requirements
for Data Submission (40 CFR $158.32), and Procedures for Claims
of Confidentiality of Data (40 CFR §158.33).  These regulations

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specify  the  format  foe  data  submitted to EPA under Section 3 of
FIFRA and Sections  408  and 409 of  FFDCA, and procedures which
must be  followed  to make  and substantiate claims of confiden-
tiality.  No entitlements to data  confidentiality are changed,
either by the  proposed  regulation  or by this notice.

     OPP is  making  these  requirements mandatory through this
Notice to gain resource-saving benefits from their use before the
entire proposed regulation becomes final.  Adequate lead time is
being provided for  submitters to comply with the new requirements.

V.   Relationship of  this Notice to Other OPP Policy and Guidance

     While this Notice  contains requirements for organizing and
formatting submittals of  supporting data, it does not address
the substance  of  test reports themselves.  "Data reporting"
guidance is  now under development  in OPP, and will specify how
the study objectives, protocol, observations, findings, and
conclusions  are organized and presented within the study report.
The data reporting  guidance  will be compatible with submittal
format requirements described in this Notice.

     OPP has also promulgated a policy (PR Notice 86-4 dated
•April 15, 1986) that  provides for  early screening of certain
applications for  registration under FIFRA §3.  The objective of
the screen is  to  avoid  the additional costs and prolonged delays
associated with handling  significantly incomplete application
packages.  As  of  the  effective date of this Notice, the screen
will include in its criteria for acceptance of application
packages the data formatting requirements described herein.

     OPP has also established a public docket which imposes dead-
lines for inserting into  the docket documents submitted in con-
nection  with Special  Reviews and Registration Standards (see
40 CFR S154.15  and  §155.32).  To meet these deadlines, OPP is
requiring an additional copy of any data submitted to the docket.
Please refer to Page  10 for  more information about this requirement,

     For several  years, OPP  has required that each application for
registration or other action include a list of all applicable
data requirements and an  indication of how each is satisfied—the
statement of the  method of support for the application.  Typically,
many requirements are satisfied by reference to data previously
submitted—either by  the  applicant or by another party.  That re-
quirement is not  altered  by  this notice, which applies only to
data submitted  with an  application.

VI.  Format  Requirements

     A more  detailed  discussion of these format requirements
follows  the  index on  the  next page, and samples of some of the
requirements are  attached.   Except for the language of the two
alternative  forms of  the  Statement of Data Confidentiality Claims
(shown in Attachment  3) which cannot be altered, these samples
are illustrative.   As long as the  required information is included
and clearly  identifiable, the form of the samples may be altered
to reflect the  submitter's preference.

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                         - INDEX -
                                                          Text Example
                                                          Page  Page
A. Organization of the Submittal Package ........    3    17

B. Transmittal Document	    4    11

C. Individual Studies  	    4

   C.I Special Considerations for Identifying studies. .    5

D. Organization of each study Volume ..........    6    17

   D.I Study Title Page	    7    12

   D.2 Statement of Data Confidentiality Claims
        (based on FIFRA S10(d)(l}) . .	    8    13
   D.3 Confidential Attachment 	 .....    8    15

   D.4 Supplemental Statement of Data Confidentiality
        Claims [other than those based on FIFRA §10(d)(l)]   8    14
   D.5 Good Laboratory Practice Compliance Statement . .    9    16

£. Reference to Previously Submitted Data  .......    9

F. Physical Format Requirements & Number of Copies ...    9
G. Special Requirements for Submitting Data to the Docket  10


A.
                     **************
Organization of Submittal Package
     A 'submittal package' consists of all studies submitted at
the same time for review in support of a single regulatory action,
along with a transmittal document and other related administrative
material (e.g. the method of support statement/ EPA Forms 8570-1,
8570-4, 8570-20, etc.) as appropriate.

     Data submitters must organize each submittal package as
described in this notice.  The transmittal and any other admin-
istrative material must be grouped together in the first physical
volume.  Each study included in the submittal package must then
be bound separately.

     Submitters sometimes provide additional materials that are
intended to clarify, emphasize, or otherwise comment to help
Product Managers and reviewers better understand the submittal.

     If such materials relate to one study, they should be
     included as an appendix to that study.

     If such materials relate to more than one study (as for
     example a summary of all studies in a discipline) or to the
     submittal in general, they must be included in the submittal
     package as a separate study (with title page and statement
     of confidentiality claims).

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B.   Transmittal Document

     The first item in each submittal package must be a trans-
mittal document.  This document identifies the submitter or all
joint submitters; the regulatory action in support of which the
package is being submitted—i.e., a registration application,
petition, experimental use permit (EUP), S3(c)(2)(B) data call-in,
§6(a){2) submittal, or a special review; the transmittal date;
and a list of all individual studies included in the package in
the order of their appearance, showing (usually by Guideline
reference number) the data requirement(s) addressed by each one.
The EPA-assigned number for the regulatory action (e.g. the
registration, EUP, or tolerance petition number) should be
included in the transmittal document as well, if it is known to
the submitter.  See Attachment 1 for an example of an acceptable
transmittal document.

     The list of included studies in the transmittal of a data
submittal package supporting a registration application should
be subdivided by discipline, reflecting the order in which data
requirements appear in 40 CFR 158.

     The list of included studies in the transmittal of a data
submittal package supporting a petition for tolerance or an
application for an EUP should be subdivided into sections A, B,
C,.... of the petition or application, as defined in 40 CFR
180.7 and 158.125, (petitions) or Pesticide Assessment Guidelines,
Subdivision I (EUPs) as appropriate.

     When a submittal package supports a tolerance petition and
an application for a registration or an EUP, list the petition
studies first, then the balance of the studies.  Within these
two groups of studies follow the instructions above.
C.
individual studies
     A study is the report of a single scientific investigation,
including all supporting analyses required for logical complete-
ness.  A study should be identifiable and distinguishable by a
conventional bibliographic citation including author, date, and
title.  Studies generally correspond in scope to a single Guide-
line requirement for supporting data, with some exceptions dis-
cussed in section C.I.  Each study included in a submittal package
must be bound as a separate entity.  (See comments on binding
studies on page 9.)

     Each study must be consecutively paginated, beginning from
the title page as page 1.  The total number of pages in the com-
plete study must be shown on the study title page.  In addition
(to ensure that inadvertently separated pages can be reassociated
with the proper study during handling or review) use either of
the following:

     - Include the total number of pages in the complete study
       on each page (ie., 1 of 250, 2 of 250, ...250 of 250).

     - Include a company name or mark and study number on each
       page of the study, e.g., Company Name-1986-23.  Never reuse
       a study number for marking the pages of subsequent studies.

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     When a single study is extremely long, binding it in multiple
volumes is permissible so long as the entire study is paginated
in a single series, and each volume is plainly identified by the
study title and its position in the multi-volume sequence.
C.1  Special Considerations for Identifying Studies

     Some studies raise special problems in study identification,
because they address Guidelines of broader than normal scope or
for other reasons.

     a. Safety studies.  Several Guidelines require testing for
safety in more than one species.  In these cases each species
tested should be reported as a separate study, and bound
separately.

    . Extensive supplemental reports of pathology reviews, feed
analyses, historical control data, and the like are often assoc-
iated with safety studies.  Whenever possible these should be
submitted with primary reports of the study, and bound with the
primary study as appendices.  When such supplemental reports are
submitted independently of the primary report, take care to fully
identify the primary report to which they pertain.

     Batteries of acute toxicity tests, performed on the same end
use product and covered by a single title page, may be bound
together and reported as a single study,

     b.   Product Chemistry Studies.  All product chemistry data
within a submittal package submitted in support of an end-use
product produced from registered manufacturing-use products
should be bound as a single study under a single title page.

     Product chemistry data submitted in support of a techni-
cal product, other manufacturing-use product, an experimental
use permit, an import tolerance petition, or an end-use prod-
uct produced from unregistered source ingredients, should be
bound as a single study for each Guideline series (61, 62,
and 63) for conventional pesticides, or for the equivalent
subject range for biorat'ional pesticides.  The first of the
three studies in a complete product chemistry submittal for
a biochemical pesticide would cover Guidelines 151-10, 151-11,
and 151-12; the second would cover Guidelines 151-13, 151-15,
and 151-16; the third would cover Guideline 151-17.  The
first study for a microbial pesticide would cover Guidelines
151-20, 151-21, and 151-22; the second would cover Guidelines
151-23 and 151-25; the third would cover Guideline 151-26.

     Note particularly that product chemistry studies are
likely to contain Confidential Business Information as defined
in FIFRA SlO(d)U)(A), (B), or  (C), and if so must be handled
as described in section D.3. of this notice.

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     c.   Residue Chemistry Studies.  Guidelines 171-4, 153-3,
and 153-4 are extremely broad in scope; studies addressing
residue chemistry requirements must thus be defined at a
level below that of the Guideline code.  The general principle,
however, of limiting a study to the report of a single inves-
tigation still applies fully.  Data should be treated as a
single study and bound separately for each analytical method,
each report of the nature of the residue in a single crop or
animal species, and for each report of the magnitude of resi-
dues resulting from treatment of a single crop or from proces-
sing a single crop.  When more than one commodity is derived
from a single crop (such as beet tops and beet roots) residue
data on all such commodities should be reported as a single
study.  When multiple field trials are associated with a
single crop, all such trials should be reported as a single
study.
                         /
D.   Organization of Each Study Volume

     Each complete study must include all applicable elements in
the list below, in the order indicated.  (Also see Page 17.)
Several of these elements are further explained in the following
paragraphs.  Entries in the column headed 'example* cite the
page number of this notice where the element is illustrated.
Element

Study Title Page

Statement of Data
Confidentiality
Claims

Certification of Good
Laboratory Practice
Flagging statements
Body of Study
Study Appendices

Cover Sheet to Confi-
dential Attachment

CBI Attachment
Supplemental Statement
of Data Confidentiality
Claims
When Required                    Example

Always                           Page 12

One of the two alternative       Page 13
forms of this statement
is always required.

If study reports laboratory      Page 16
work subject to GLP require-
ments .

For certain toxicology studies. (When
flagging requirements are finalized.)

Always - with an English language
translation if required.

At submitter's option
If CBI is claimed under FIFRA
S10(d)(l)(A), (B), or (C)

If CBI is claimed under FIFRA
  S10(d)(l)(A), (B), or (C)

Only if confidentiality is
claimed on a basis other than
FIFRA S10(d)(l)(A), (B), or (C)
Page 15


Page 14

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D.I  Title Page

     A title page  is always  required for each submitted study,
published or unpublished.  The title page mast always be freely
releasable to requestors; DO NOT  INCLUDE CBI ON THE TITLK PAGE.
An example of an acceptable  title page is on page 12 of this
notice.  The following information must appear on the title
page:

a..   Study title.  The study title should be as descriptive as
possible,  it must clearly identify the substance(s) tested and
correspond to the  name of the data requirement as it appears
in the Guidelines.

b.   Data requirement addressed.  Include on the title page the
Guideline number{s) of the specific requirement(s) addressed by
the study.

c.   Author(s).  Cite only individuals with primary intellectual
responsibility for the content of the study.  Identify them
plainly as authors, to distinguish them from the performing
laboratory, study  sponsor, or other names that may also appear on
the title page.

d.   Study Date.   The title  page must include a single date for
the utudy.  If parts of the  study were performed at different
times, use only the date of  the latest element in the study.

e.   Performing Laboratory Identification.  If the study reports
work done by one or more laboratories, include on the title page
the name and address of the  performing laboratory or laboratories,
and the laboratory's internal project number(s) for the work.
Clearly distinguish the laboratory's project identifier from any
other reference numbers provided  by the study sponsor or submitter.

f.   Supplemental  Submissions.  If the study is a commentary on
or supplement to another previously submitted study, or if it
responds to EPA questions raised  with respect to an earlier study,
include on the title page elements a. through d. for the previously
submitted study, along with  the EPA Master Record Identifier (MRID)
or Accession number of the earlier study if you know these numbers.
(Supplements submitted in the same submittal package as the primary
study should be appended to  and bound with the primary study.  Do
not include supplements to more than one study under a single
title page).

g.   Facts of Publication.   If the study is a reprint of a pub-
lished document, identify on the  title page all relevant facts
of publication, such as the  journal title, volume, issue, inclusive
page numbers, and  publication date.

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D.2. Statements of Data Confidentiality Claims Under FIFRA S10(d)(l).

     Each submitted study must be accompanied by one of the two
alternative forms of the Statement of Data Confidentiality Claims
specified in the proposed regulation in $158.33 (b) and (c).
(See Attachment 3) These statements apply only to claims of data
confidentiality based on FIFRA S10(d)(1)(A), (B), or (C).  Use
the appropriate alternative form of the statement either to
assert a claim of §10(d)(l) data confidentiality (§158.33(b))
or to waive such a claim ($158.33(c)).  In either case, the
statement must be signed and dated, and must include the typed
name and title of the official who signs it.  Do not make CBI
claims with respect to analytical methods associated with petitions
for tolerances or emergency exemptions (see NOTE pg 13).

0,3. Confidential Attachment

     If the claim is made that a study includes confidential busi-
ness information as defined by the criteria of FIFRA §10(d)(1)(A),
(B), or (C) (as described in D.2. above) all such information must
be excised from the body of the study and confined to a separate
study-specific Confidential Attachment.  Each passage of CBI so
isolated must be identified by a reference number cited within the
body of the study at the point from which the passage was excised
(See Attachment 5).

     The Confidential Attachment to a study must be identified by a
cover sheet fully identifying the parent study, and must be clearly
marked 'Confidential Attachment.1  An appropriately annotated
photocopy of the parent study title page may be used as this cover
sheet.  Paginate the Confidential Attachment separately from the
body of the study, beginning with page 1 of X on the title page.
Each passage confined to the Confidential Attachment must be assoc-
iated with a specific cross reference to the page(s) in the main
body of the study on which it is cited, and with a reference to the
applicable passage(s) of FIFRA §10(d){l) on which the confidentiality
claim is based.

D.4. Supplemental Statement of Data Confidentiality Claims  (See
     Attachment 4)

     If you wish to make a claim of confidentiality for any
portion of a submitted study other than described by FIFRA S10(d)
(1)(A), (B), or (C), the following provisions apply:

  -  The specific information to which the claim applies must be
     clearly marked in the body of the study as subject to a
     claim of confidentiality.

  -  A Supplemental statement of Data Confidentiality Claims
     must be submitted, identifying each passage claimed confi-
     dential and describing in detail the basis for the claim.
     A list of the points to address in such a statement is
     included in Attachment 4 on Pg 14.

  -  The Supplemental Statement of Data Confidentiality Claims
     must be signed and dated and must include the typed name
     and title of the official who signed it.

                                8

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D.5  Good Laboratory Practice Compliance statement

     This statement is required if the study contains laboratory
work subject to GLP requirements specified in 40 CFR 160.  Sam-
ples of these statements are shown in Attachment 6.

E.   Referenceto Previously Submitted Data

     DO NOT RESUBMIT A STUDY THAT HAS PREVIOUSLY BEEN SUBMITTED
FOR ANOTHER PURPOSE unless EPA specifically requests it.  A copy
of the title page plus the MRID number (if known) is sufficient
to allow us to retrieve the study immediately for review.  This
prevents duplicate entries in the Agency files, and saves you
the cost of sending more copies of the study.  References to pre-
viously submitted studies should not be included in the transmit-
tal document, but should be incorporated into the statement of
the method of support for the application.

F.   Physical Format Requirements

     All elements in the data submittal package must be on uniform
8 1/2 by 11 inch white paper, printed on one side only in black
ink, with high contrast and good resolution.  Bindings for indi-
vidual studies must be secure, but easily removable to permit
disassembly for microfilming.  Check with EPA for special
instructions before submitting data in any medium other than
paper, such as film or magnetic media.

Please be particularly attentive to the following points:

  o  Do not include frayed or torn pages.

  o  Do not include carbon copies, or copies in other than
     black ink.

  o  Make sure that photocopies are clear, complete, and fully
     readable.

  o  DO not include oversize computer printouts or fold-out pages.

  o  Do not bind any documents with glue or binding tapes.

  o  Make sure that all pages of each study, including any attach-
     ments or appendices, are present and in correct sequence.

     Number of Copies Required - All submittal packages except
those associated with a Registration Standard or Special Review
(see Part G below) must be provided in three complete, identical
copies.  (The proposed regulations specified two copies; three
are now being required to expedite and reduce the cost of proces-
sing data into the OPP Pesticide Document Management System and
getting it into review.)

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G.   Special Requirements for Submitting Data to the Docket

     Data submittal packages associated with a Registration Stan-
dard or Special Review must be provided in four copies, from one
of which all material claimed as CBI has been excised.  This
fourth copy will become part of the public docket for the RS or
SR case.  If no claims of confidentiality are made for the study,
the fourth copy should be identical to the other three.  When
portions of a study submitted in support of an RS or SR are
claimed as CBI, the first three copies will include the CBI
material as provided in section D of this notice.  The following
special preparation is required for the fourth copy.

  o  Remove the 'Supplemental Statement of Data Confidentiality
     Claims1.

  o  Remove the 'Confidential Attachment*.

  o  Excise from the body of the study any information you claim
     as confidential, even if it does not fall within the scope
     of FIFRA S10(d)(l)(A) , (B), or CO.  Do not close up or
     paraphrase text remaining after this excision.

  o  Mark the fourth copy plainly on both its cover and its title
     page with the phrase "Public Docket Material - contains no
     information claimed as confidential".
V.
For Further Information
     For further information contact William C. Grosse, Chief,
Information Services Branch, Program Management and Support
Division, (703-557-2613).
                            ames W. Akerman
                           'Acting Director,
                           Registration Division
Attachment 1.
Attachment 2.
Attachment 3.
Attachment 4.
Attachment 5.
Attachment 6.
Attachment 7.
         Sample Transmittal Document
         Sample Title Page for a Newly Submitted Study
         Statements of Data Confidentiality Claims
         Supplemental Statement of Data Confidentiality Claims
         Samples of Confidential Attachments
         Sample Good Laboratory Practice Statements
         Format Diagrams for Submittal Packages and Studies
                                10

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                          ATTACHMENT 1.

      ELEMENTS TO BE INCLUDED IN THE TRANSMITTAL DOCUMENT*



!• Name and address of submitter (or all joint submitters**)

   . *Smith Chemical Corporation         Jones Chemical Company
     1234 West Smith Street      -and-  5678 Wilson Blvd
     Cincinnati, OH 98765               Covington, KY 56789

    ^Smith Chemical Corp. will act as sole agent foe all submitters.

2. Regulatory action in support of which this package is submitted

   Use the EPA identification number (e.g. 359-EUP-67) if you
   know it Otherwise describe the type of request (e.g. experi-
   mental use permit, data call-in - of xx-xx-xx date).

3. Transmittal date

4. List of submitted studies

   Vol 1.  Administrative materials - forms, previous corres-
           pondence with Project Managers, and so forth.

   Vol 2.  Title of first study in the submittal (Guideline No.)
     *
     •
     •
   Vol n.  Title of nth study in the submittal (Guideline No.)

   *   Applicants commonly provide this information in a trans-
       mittal letter.  This remains an acceptable practice so
       long as all four elements are included.

   **  Indicate which of the joint submitters is empowered to
       act on behalf of all joint submitters in any matter con-
       cerning data compensation or subsequent use or release
       of the data.
Company Official:
Company Name:
Company Contact:
                  Name
Signature
                  Name
        Phone
                               11

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                   ATTACHMENT 2.

SAMPLE STUDY TITLE PAGE FOR A NEWLY SUBMITTED STUDY




                    Study Title

  (Chemical name) -  Magnitude of Residue on Corn


                  Data Requirement

                  Guideline 171-4


                       Author

                   John C. Davis


                 Study Completed On

                  January 5, 1979


               Performing Laboratory

           ABC Agricultural Laboratories
                 940 West Bay Drive
                Wilmington, CA 39897


               Laboratory Project ID

                     ABC 47-79
                    Page 1 of X
   (X is the total number of pages in the study)
                         12

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                                 ATTACHMENT 3.

                   STATEMENTS OF DATA CONFIDENTIALITY CLAIMS




1.  No claim of confidentiality under FIFRA §10{d)(l)(A),(B),  or (C)


                    STATEMENT OF NO DATA CONFIDENTIALITY CLAIMS
   No claim of confidentiality is made for any information contained in this study
   on the basis of its falling within the scope of FIFRA S10(d)(l)(A),  (B),  or (C).
   Company
   Company Agent: 	Typed Name                     Date:

   	Title	    	Signature
2.  Claim of confidentiality under FIFRA S10(d)(l)(A), (B),  or (C)»
                    STATEMENT OF DATA CONFIDENTIALITY CLAIMS
   Information claimed confidential on the basis of its falling within the scope
   of FIFRA SlO(d)(l)(A), (B), or (C) has been removed to a confidential appendix,
   and is cited by cross-reference number in the body of the study.
   Conpany:
   Company Agent:	Typed Name	     Date:

   	Title	'       	Signature
  NOTE:  Applicants for permanent or temporary tolerances should note that it
  is OPP policy that no permanent tolerance, temporary tolerance, or request for
  an emergency exemption incorporating an analytical method, can be approved
  unless the applicant waives all claims of confidentiality for the analytical
  method.  These analytical methods are published in the FDA Pesticide Analyt-
  ical Methods Manual, and therefore cannot be claimed as confidential.  OPP
  implements this policy by returning submitted analytical methods, for which
  which confidentiality claims have been made, to the submitter, to obtain
  the confidentiality waiver before they can be processed.


                                      13

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-------
                        ATTACHMENT 4.

    SUPPLEMENTAL STATEMENT OF DATA CONFIDENTIALITY CLAIMS
     For any portion of a submitted study that is not described
by FIFRA S10(d)(l)(A), (B), or (C), but for which you claim
confidential treatment on another basis, the following informa-
tion must be included within a Supplemental Statement of Data
Confidentiality Claims:

  o  Identify specifically by page and line number(s) each
     portion of the study for which you claim confidentiality.

  o  Cite the reasons why the cited passage qualifies for
     confidential treatment.

  o  Indicate the length of time—until a specific date or
     event, or permanently—for which the information should
     be treated as confidential.

  o  Identify the measures taken to guard against undesired
     disclosure of this information.

  o  Describe the extent to which the information has been
     disclosed, and what precautions have been taken in con-
     nection with those disclosures.

  o  Enclose copies of any pertinent determinations of confi-
     dentiality made by EPA, other Federal agencies, or courts
     concerning this information.

  o  If you assert that disclosure of this information would
     be likely to result in substantial harmful effects to
     you, describe those harmful effects and explain why they
     should be viewed as substantial.

  o  If you assert that the information is voluntarily sub-
    . mitted, indicate whether you believe disclosure of this
     information might tend to lessen the availability to
     EPA of similar information in the future, and if so, how.
                              14

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-------
                                    ATTACHMENT 5.

                     EXAMPLES OP SEVERAL CONFIDENTIAL ATTACHMENTS

(Example 1  (Confidential word or phrase that has  been deleted frcm the study)
 CROSS REFERENCE NUMBER
 DELETED WORDS OR PHRASE:
                      This cross reference number is used in the study
                      in place of the following words or phrase at the
                      indicated volume and page references.

                       Ethylene Glycol	
   PAGE  LINE   REASON FOR THE DELETION
                                                       FIFRA REFERENCE
     6
    28
   100
14
25
19
Identity of Inert Ingredient
 Example 2  (Confidential paragraph(s)  that have been deleted from the study)
CROSS REFERENCE NUMBER   5    This cross reference number is used in the study
                               in place of the following paragraph(s) at the
                               indicated volume and page references.

DELETED PARAGRAPH(S):
                   Reproduce the deleted paragraph(s)  here
   PAGE  LINES  REASON FOR THE DELETION

    20    4-17    Description of the quality control process
                                                       FIFRA REFERENCE
Example  3   (Confidential pages that have been deleted from the study)
CROSS REFERENCE NUMBER   7    This cross reference number noted on a place-holder
                               page is used in place of the following whole pages
                               at the indicated volume and page references.

DELETED PAGE(S):  are attached immediately behind this page.

   PAGE(S)       REASON FOR THE DELETION                          FIFRA REFERENCE

    33-41        Description of product manufacturing process
                                         15

-------

-------
                          ATTACHMENT 6.
            SAMPLE GOOD LABORATORY PRACTICE STATEMENTS
Example 1.
   This study meets the requirements for 40 CFR Part  160
      Submitter 	
      Sponsor 	
      Study Director 	
Example 2.
  This study does not meet the requirements of 40 CFR
  Part 160, and differs in the following ways:
  1.
  2.
  3.
     Submitter
     Sponsor
     Study Director
Example 3.
   The submitter of this study was neither the sponsor of this
   study nor conducted it, and does not know whether it has
   been conducted in accordance with 40 CFR Part 160.
     Submitter
                               16

-------

-------
                        ATTACHMENT 7.
                FORMAT OF THE SUBMITTAL PACKAGE
                  Transmittal Document.
                      Related Administrative Materials
                      (e.g., Method of Support Statement, etc.)
                            Other materials about the submittal
                            (e.g. , summaries of groups of studies
                            to aid in their review).
                                   Studies, submitted as unique
                                   physical entities, according
                                _^ to the format below.
                  FORMAT OF SUBMITTED STUDIES
           Study title page.

               Statement of Confidentiality Claims.

                _.. GLP and flagging* statements - as appropriate.

                        Body of the study, with English
                      -••" language translation if required.

                            Appendices to the study.

                                Title Page of the Confidential
                            -•'•" Attachment.

                                     Confidential Attachment.

                                         Supplemental Statement
                                   	—• of Confidentiality Claims,

                                     * When flagging requirements
                                       are finalized.
                Documents which must be submitted as
                appropriate to meet established requirements.
                1
                     Documents submitted at submitter's option.

                             17
LEGEND

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BOZEMAN BIO-TECH INC
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the inert ingredients in the

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               ATTACHMENT D

EPA GROUPING OF END-USE PRODUCTS FOR MEETING
    DATA REQUIREMENTS FOR REREGISTRATION

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EPA'S BATCHING OF END-USE PRODUCTS CONTAINING N. locustae AS THE
ACTIVE INGREDIENT FOR MEETING ACUTE TOXICITY DATA
REQUIREMENTS FOR REREGISTRATION

    In an effort to reduce the time, resources and number of animals needed to fulfill the acute
toxicity data requirements for reregistration of end-use products containing the active ingredient
N. locustae, the Agency has batched products which can be considered similar in terms of acute
toxicity.    Factors considered  in the sorting process include each product's active and inert
ingredients (identity, percent composition and biological activity), type of formulation (e.g.,
emulsifiable concentrate,  aerosol,  wettable powder, granular, etc.), and labeling (e.g., signal
word, use classification, precautionary labeling, etc.). Note that the Agency is not describing
batched products as  "substantially similar" since some products within a batch may not be
considered  chemically similar or have identical use patterns.

    Batching has been accomplished using the readily available information described above, and
frequently acute toxicity data on individual end-use products has been found to be incomplete.
Notwithstanding the batching process, the Agency reserves the right to require, at any time,
acute toxicity data for an individual end-use product should the need arise.

     Registrants of end-use products within a batch may choose to cooperatively generate, submit
or cite a single battery of six acute lexicological studies to represent all the products within that
batch.   It is the registrants* option to participate in the process with all other registrants, only
some of the other registrants, or only their own products within a batch, or to generate all the
required acute toxicological studies for each of their own products.  If a registrant chooses to
generate  the data for a batch, he/she must use one of the products within the batch as the test
material. If a registrant chooses to rely upon previously submitted acute toxicity data, he/she
may do so provided that the data base is complete and valid by today's standards (see acceptance
criteria attached), the formulation tested is considered by EPA to be similar for acute toxicity,
and the formulation has not been significantly altered since submission and acceptance of the
acute toxicity data.   Regardless of whether new data is generated or existing data is referenced,
registrants  must clearly identify the test material by EPA Registration Number.

    In deciding how to meet the product specific data requirements, registrants must follow the
directions given in the Data Call-In Notice and its attachments appended to the RED. The DCI
Notice contains two  response forms which are to be completed and submitted to the Agency
within 90 days of receipt. The first form, "Data Call-In Response," asks whether the registrant
will meet the data requirements for each product.  The second form, "Requirements Status and
Registrant's Response," lists the product specific data required for each product, including the
standard  six acute toxicity tests. A registrant who wishes to participate in a batch must decide
whether he/she will  provide the data or depend  on someone else to do  so.  If a registrant
supplies the data to support a batch of products, he/she must select one of the following options:
Developing Data (Option 1), Submitting an Existing Study (Option 4), Upgrading an Existing
Study (Option 5) or Citing an Existing Study (Option 6).  If a registrant depends on another's
data, he/she must choose among: Cost Sharing (Option 2), Offers to Cost  Share (Option 3) or
Citing an Existing Study (Option 6).   If a registrant does not want to participate in a batch, the
choices are Options 1, 4, 5 or 6.   However, a registrant should know that choosing not to

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participate in a batch does not preclude other registrants in the batch from citing his/her studies
and offering to cost share (Option 3) those studies.
Table 1
Batch
1
EPA Reg. No.
36488-3
36488-7
46149-2
54735-5
% N. locustae
0.049%
0.08%
0.05%
0.001%
Formulation Type
wettable powder
wettable powder
bait / dust
bait
       Table 2 lists those products the Agency was unable to batch.  These products were either
considered not to be similar to other products for purposes of acute toxicity or the Agency
lacked sufficient information for decision making.   Registrants of these products are responsible
for meeting the acute toxicity data requirements for each product.

Table 2
EPA Reg. No.
46149-01
54735-3
% N. locustae
0.10%
0.05%
Formulation Type
emulsifiable concentrate
ready-to-use solution

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     ATTACHMENT E
EPA ACCEPTANCE CRITERIA

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                          SUBDIVISION D
Guideline

Series 61
Series 62
Series 63
        Study Title

Product Identity and Composition
Analysis and Certification of Product Ingredients
Physical and Chemical Characteristics

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               61 Product Identity and Composition
                       ACCEPTANCE CRITERIA
Does your study meet the following acceptance criteria?

1.	 Name of technical material tested (include product name and
      trade name, if appropriate)

2.	 Name,  nominal concentration,  and certified limits (upper and
      lower) for each active ingredient and each intentionally-
      added inert ingredient

3.	 Name and upper certified limit for each impurity or each
      group of impurities present at > 0.1% by weight and for
      certain toxicologically significant impurities (e.g.,
      dioxins, nitrosamines) present at <0.1%

4.	 Purpose of each active ingredient and each intentionally-
      added inert

5.	 Chemical name from Chemical Abstracts index of Nomenclature
      and Chemical Abstracts Service (CAS)  Registry Number for each
      active ingredient and, if available, for each intentionally-
      added inert

6.	 Molecular,  structural, and empirical formulas, molecular
      weight or weight range, and any company assigned experimental
      or internal code numbers for each active ingredient

7.	 Description of each beginning material in the manufacturing
      process
      	 EPA Registration Number if registered;  for other
           beginning materials,  the following:
      	 Name and address of manufacturer or supplier
      	 Brand name,  trade name or commercial designation
      	 Technical specifications or data sheets by which
           manufacturer or supplier describes composition,
           properties or toxicity

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8.	 Description of manufacturing process
      	 Statement of whether batch or continuous process
      	 Relative amounts of beginning materials and order in
           which they are added
      	 Description of equipment
      	 Description of physical conditions (temperature,
           pressure, humidity) controlled in each step and the
           parameters that are maintained
      	 Statement of whether process involves intended chemical
           reactions
      	 Flow chart with chemical equations for each intended
           chemical reaction
      	 Duration of each step of process
      	 Description of purification procedures
      	Description of measures taken to assure quality of final
           product

9.	 Discussion of formation of impurities based on established
      chemical theory addressing (1) each impurity which may be
      present at > 0.1% or  was found at > 0.1% by product analyses
      and (2) certain toxicologically significant impurities
      (see #3)

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                61 Product Identity and  Composition


               GUIDANCE FOR SUMMARIZING STUDIES


 The following criteria apply  to the technical  grade  of  the  active
 ingredient being  reregistered.   Items 1,  2, 3,  and  5  can  be
 satisfied for  most  registered  products  by  submission  of  the
 Certified Statement of Formula  Ingredients Page (EPA Form 8570-4).
 Items 7 and  8  can be satisfied  for most  technical grade  active
 ingredients  (TGAIs)  by submission  of a flow chart  with chemical
 equations for each  intended  chemical reaction.   The  flow  chart
 should include complete  chemical structures  and  names for  each
 reactant  and  product  of all the reactions.


 1. Name of technical material  (include product name and trade name,
    if appropriate).
 2.  Description  of each active and intentionally-added  inert
    ingredient, including name, concentration, and certified limits.
 3.  Name and upper limit for all impurities present at > 0.1% and
    those  toxicologically  significant impurities present at  
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       62 Analysis  and  Certification of Product Ingredients
                       ACCEPTANCE CRITERIA

The following criteria apply to the technical grade of the active
ingredient  being  reregistered.    Use  a  table  to  present  the
information in items 6, 7, and 8.

Does your study meet the following acceptance criteria?

 1.	 Five or more representative samples (batches in case of
       batch process) analyzed for each active ingredient and all
       impurities present at > 0.1%

 2.	 Degree of accountability or closure > ca 98%

 3.	 Analyses conducted for certain trace toxic impurities at
       lower than 0.1% (examples, nitrosamines in the case of
       products containing dinitroanilines  or containing secondary
       or tertiary amines/alkanolamines plus nitrites;
       polyhalogenated dibenzodioxins and dibenzofurans)  [Note
       that in the case of nitrosamines both fresh and stored
       samples must be analyzed.]

 4.	Complete and detailed description of each step in analytical
       method used to analyze above samples

 5.	 Statement of precision and accuracy of analytical method
       used to analyze above samples

 6.	 Identities and quantities (including mean and standard
       deviation) provided for each analyzed ingredient

 7.	 Upper and lower certified limits proposed for each active
       ingredient and intentionally added inert along with
       explanation of how the limits were determined

 8.	 Upper certified limit proposed for each impurity present at
       > 0.1% and for certain toxicologically significant
       impurities at <0.1% along with explanation of how limit
       determined

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 9-	 Analytical methods to verify certified limits of each
       active ingredient and impurities (latter not required if
       exempt from requirement of tolerance or if generally
       recognized as safe by FDA) are fully described

10«	 Analytical methods (as discussed in #9} to verify certified
       limits validated as to their precision and accuracy

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       62  Analysis and Certification of  Product  Ingredients


                 GUIDANCE FOR SUMMARIZING STUDIES


The following criteria apply to the technical grade of the active
ingredient being reregistered.


 1. Number of representative samples analyzed for all active
    ingredients and all impurities at > 0.1%.

 2. Degree of accountability or closure in analyses in item #1.

 3. Chemical names of toxic impurities which were analyzed for
    levels <0.l%.

 4. Brief description(s) of analytical method(s) used to measure
    active ingredients and impurities in items #1 and #3.

 '5. Statement of precision and accuracy of method(s) in item #4.

 6. Chemical name and quantities observed (range,, mean, standard
    deviation) for each ingredient  (actives and impurities)
    analyzed in item #1.

 7. Proposed upper and lower certified limits for each active
    ingredient and intentionally added inert with brief explanation
    of how limits were determined.

 8. Proposed upper certified limit for each impurity present at
    >=0.l% and certain toxicologically significant impurities at
    <0.1% with brief explanation of how limits were determined.

 9. Brief description of analytical method(s) used to verify
    certified limits  (if same methods as item #4,  may reference
    latter).

10. Statement of precision and accuracy of method(s) in item #9
    (may reference item #5 if applicable).

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             63 Physical and Chemical  Characteristics


                       ACCEPTANCE CRITERIA


The following criteria apply to the technical grade of the active
ingredient being reregistered.

Does your study meet the following acceptance criteria?

63-2 Color
     	 Verbal description of coloration (or lack of it)
     	 Any intentional coloration also reported in terms of
         Munsell color system

63-3 Physical State
     	 Verbal description of physical state provided using terms
         such as "solid, granular, volatile liquid"
     	 Based on visual inspection at about 20-25° c

63-4 Odor
     	 Verbal description of odor (or lack of it) using terras
         such as "garlic-like, characteristic of aromatic
         compounds"
     	 Observed at room temperature

63-5 Melting Point
     	 Reported in C°
     	 Any observed decomposition reported

63-6 Boiling Point
     	 Reported in C°
     	 Pressure under which B.P. measured reported
     	 Any observed decomposition reported

63-7 Density, Bulk Density, Specific Gravity
     	 Measured at about 20-25° C
     	 Density of technical grade active ingredient reported in
         g/ml or the specific gravity of liquids reported with
         reference to water at 20° c.  [Note: Bulk density of
         registered products may be reported in Ibs/ft  or
         Ibs/gallon.]

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63-8 Solubility
     	Determined in distilled water and representative polar and
         non-polar solvents, including those used in formulations
         and analytical methods for the pesticide
     	 Measured at about 20-25° C
     	 Reported in g/100 ml (other units like ppm acceptable if
         sparingly soluble)

63-9 Vapor Pressure
     	 Measured at 25° C (or calculated by extrapolation from
         measurements made at higher temperature if pressure too
         low to measure at 25° C)
     	 Experimental procedure described
     	 Reported in mm Hg (torr) or other conventional units

63-10 Dissociation Constant
     	 Experimental method described
     	 Temperature of measurement specified (preferably
         about 20 - 25" C)

63-11 Octanol/water Partition Coefficient
     	 Measured at about 20-25° c
     	 Experimentally determined and description of procedure
         provided .(preferred method-45 Fed. Register 77350)
     	 Data supporting reported value provided

63-12 pH
     	 Measured at about 20 - 25° C
     	 Measured following dilution or dispersion in distilled
         water

63-13 Stability
     	 Sensitivity to metal ions and metal determined
     	 Stability at normal and elevated temperatures
     	 Sensitivity to sunlight determined

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             63  Physical  and Chemical Characteristics


                 GUIDANCE FOR SUMMARIZING  STUDIES


The following criteria apply to the technical grade of the active
ingredient being reregistered.


 1. Description of color.
 2. Description of physical state.
 3. Description of odor.
 4. Indication of melting point (in C°).
 5. Indication of boiling point (in C°) .
 6. Indication of density, bulk density, and specific gravity.
 7. Indication of solubility.
 8. Indication of vapor pressure.
 9. Indication of dissociation constant.
10. Indication of octanol/water partition coefficient.
11. Indication of PH.
12. Description of stability.

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                          SUBDIVISION F
Guideline              Study Title

  81-1      Acute Oral Toxicity in the Rat
  81-2      Acute Dermal Toxicity in the Rat, Rabbit or Guinea Pig
  81-3      Acute Inhalation Toxicity in the Rat
  81-4      Primary Eye Irritation in the Rabbit
  81-5      Primary Dermal Irritation study
  81-6      Dermal Sensitization in the Guinea Pig
  81-7      Acute Neurotoxicity in the Hen

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               81-1 Acute Oral Toxicity in the Rat


                       ACCEPTANCE CRITERIA


Does your study meet the following acceptance criteria?

 1.	 Identify material tested (technical, end-use product, etc)
 2.	 At least 5 young adult rats/sex/group
 3.	 Dosing, single oral may be administered over 24 hrs.
 4..*	 Vehicle control if other than water.
 5.	 Doses tested, sufficient to determine a toxicity category
        or a limit dose (5000 mg/kg).
 6.	 Individual observations at least once a day.
 7.	 Observation period to last at least 14 days, or until all
        test animals appear normal whichever is longer.
 8.	 Individual daily observations.
 9.	 Individual body weights.
10.	 Gross necropsy on all animals.
Criteria marked with a * are supplemental and may not be required
for every study.

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              81-1 Acute Oral Toxicity in the Rat


               GUIDANCE  FOR  SUMMARIZING  STUDIES
1. The form of pesticide tested, e.g.  solid,  liquid,  percent
   AI in technical, end-use product,  etc.
2. The number of animals/dose/sex tested.
3. Dosing route and regimen.
4. Vehicle used
5. Doses tested and results
6. Individual observations on day of dosing and for at
   least 14 days.
7. Summarization of body weights
8. Summarization of gross necropsy
9. Significance of changes from the Acceptance Criteria

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   81-2 Acute Dermal toxicity in the Rat, Rabbit or Guinea Pig


                       ACCEPTANCE CRITERIA


Does your study meet the following acceptance criteria?

 1.	Identify material tested (technical,  end-use product, etc)

 2.	 At least 5 animals/sex/group
 3.i	 Rats 200-300 gin, rabbits 2.0-3.0 kg or guinea pigs 350-
         450 gm.
 4.	 Dosing, single dermal.
 5.	 Dosing duration at least 24 hours.
 6..1	 Vehicle control, only if toxicity of vehicle is unknown.
 7.	 Doses tested,  sufficient to determine a toxicity category
         or a limit dose (2000 mg/kg).
 8.	 Application site clipped or shaved at least 24 hours
         before dosing
 9.	 Application site at least 10% of body surface area.
10.	Application site covered with a porous nonirritating cover
         to retain test material and to prevent ingestion.
11.	 Individual observations at least once a day.
12.	 Observation period  to last at least 14  days.
13.	 Individual body weights.
14.	 Gross necropsy on all animals.
Criteria marked with a * are supplemental and may not be required
for every study.

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   81-2 Acute Dermal Toxicity in the Rat, Rabbit or Guinea Pig


                GUIDANCE  FOR  SUMMARIZING STUDIES


 1. The form of pesticide tested, e.g., solid, liquid, percent AI
    in technical,  end-use product, etc.
 2. The number of animals/sex/dose
 3. Weight range of animals
 4. Verification of single, dermal exposure
 5. Duration of dermal exposure
 6. Statement of vehicle control
 7. Doses tested and results
 8. Preparation of application site
 9. Area of application site (percent body surface)
10. Occlusion of test material on application site
11. Individual observations on day of dosing and for at
    least 14 days  or until all animals appear normal (whichever is
    longer).
12. Summarization of body weights
13.. Summarization of gross necropsy
14. Significance of changes from Acceptance Criteria

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            81-3 Acute Inhalation Toxicity in the Rat
                       ACCEPTANCE CRITERIA
Does your study meet the following acceptance criteria?

 1.	 Identify material tested  (technical, end-use product, etc)
 2.	 Product is a gas., a solid which may produce a significant
        vapor hazard based on toxicity and expected use or contains
        particles of inhalable size for man (aerodynamic diameter
        15 urn or less).
 3.	 At least 5 young adult rats/sex/group
 4.	 Dosing, at least 4 hours by inhalation.
 5.	 Chamber air flow dynamic,  at least 10 air changes/hour, at
        least 19% oxygen content.
 6.	 Chamber temperature,  22"  C (±2), relative humidity 40-60%.
 7.	 Monitor rate of air flow
 8.	Monitor actual concentrations of test material in breathing
        zone.
 9.	 Monitor aerodynamic particle size for aerosols.
10.	 Doses tested, sufficient to determine a toxicity category
        or a limit dose (5 mg/L actual concentration of respirable
        substance).
11.	 Individual observations at least once a day.
12.	 Observation period to last at least 14 days.
13.	 Individual body weights.
14.	 Gross necropsy on all animals.

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-------
           81-3 Acute Inhalation Toxicity in the Rat
                 GUIDANCE FOR SUMMARIZING  STUDIES
 1. The form of pesticide tested, e.g., solid, liquid, percent AI
    in technical, end-use product, etc.
 2. Statement of the inhalability of test substance
 3. The number of animals/sex/dose
 4. Duration of inhalation exposure
 5. Number of chamber air changes/hour and the percent oxygen
    content of chamber air
 6. Ranges for chamber air temperature and relative humidity
 7. Air flow rate
 8. Analytical concentrations of test material in breathing zone
 9. Results of aerosol particle-size determination
10. Doses tested (or limit dose of 5mg/L or highest attainable)
11. Individual observations on day of dosing and for at least 14
    days.
12. Summarization of body weights
13. Summarization of gross necropsy
14. Significance of changes from Acceptance Criteria

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            81-4 Primary Eye Irritation in the Rabbit
                       ACCEPTANCE CRITERIA
Does your study meet the following acceptance criteria?

 1.	 Identify material tested (technical, end-use product, etc)
 2.	 Study not required if material  is corrosive, causes severe
        dermal irritation or has a pH of < 2 or > 11.5.
 3.	 6 adult rabbits
 4.	 Dosing, instillation into the conjunctival sac of one eye
        per animal.
 5.	 Dose, 0.1 ml if a liquid; 0.1 ml or not more than 100 mg if
        a solid, paste or particulate substance.
 6.	 Solid or granular test material ground to a fine dust.
 7.	 Eyes not washed for at least 24 hours.
 8.	 Eyes examined and graded for irritation before dosing and
        at 1, 24,  48 and 72 hr, then daily until eyes are normal or
        21 days (whichever is shorter).
        individual daily observations.
Criteria marked with a * are supplemental and may not be required
for every study.

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           81-4  Primary Eye  Irritation  in the Rabbit


                 GUIDANCE  FOR SUMMARIZING STUDIES


 1. The form of pesticide tested, e.g., solid,  liquid, percent AI
    in technical, end-use product, etc.
 2. State if material is corrosive, cause severe dermal irritation
    or has a pH of <2 or >11.5
 3. Number of adult rabbits tested
 4. State method of dosing,  i.e., instillation into the
    conjunctival sac of one eye per animal
 5. Dose administered
 6. Note whether solid or granular test material has been ground to
    a fine dust
 7. State whether eyes were washed and at what time post
    instillation (not less than 24 hours)
 8. State whether eyes were examined and graded for irritation
    before dosing and at what periods after dosing
 9. Individual daily observations afterwards, until eyes are normal
    or for 21 days
10. Significance of changes from Acceptance Criteria

-------

-------
               81-5  Primary  Dermal  Irritation  Study


                       ACCEPTANCE CRITERIA


Does your study meet the following acceptance criteria?

 1.	Identify material tested (technical, end-use product, etc)
 2.	 Study not required if material is corrosive or has a
         pH of <2 or > 11.5.
 3.	 6 adult animals.
 4.	 Dosing, single dermal.
 5.	 Dosing duration 4 hours.
 6.	Application site shaved or clipped at  least 24 hours prior
         to dosing
 7.	Application site approximately 6 cm.
 8.	Application site covered with a gauze patch held in place
         with nonirritating tape
 9.	 Material removed,  washed with water,  without trauma to
         application site
10.	 Application site examined  and graded  for irritation at 1,
         24, 48 and 72 hr,  then daily until normal or 14 days
         (whichever is shorter).
11.i	 Individual daily observations.
Criteria marked with a * are supplemental and may not be required
for every study.

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               81-5  Primary  Dermal  Irritation  Study


                 GUIDANCE  FOR  SUMMARIZING STUDIES


 1. The form of pesticide tested, e.g., solid, liquid, percent AI
    in technical, end-use product,  etc.
 2. State if material is corrosive,  has a pH <2 or >11.5, or has a
    dermal LD 50 <200 mg/kg
 3. Number of adult animals tested
 4. Amount applied
 5. Duration of dermal exposure
 6. Preparation of application site (shaved  or  clipped at specified
    time before dosing)
 7. Area of application site
 8. Method for occlusion of application site
 9. Note removal of  test material and if skin was washed with water
10. State times post application when site  was graded for
    irritation
11. Individual observations for day of dosing and individual
    daily observations thereafter
12. Significance of changes from Acceptance Criteria.

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           81-6 Dermal Sensitization in the Guinea Pig


                       ACCEPTANCE CRITERIA


dose your study meet the following acceptance criteria?

1.	 Identify material tested  (technical, end-use product, etc)
2.	 Study not required if material is corrosive or has a
       pH of <2 or > 11.5.
3.	 One of the following methods is utilized;
             Freund's complete adjuvant test
             Guinea pig maximization test
             Split adjuvant technique
             Buehler test
             Open epicutaneous test
       	 Mauer optimization test
       	 Footpad technique in guinea pig
4.	 Complete description of test
5.±	 Reference for test.
6.	 Test followed essentially as described in reference
       document.
7.	 Positive control included (may provide historical data
       conducted within the last 6 months)
Criteria marked with a * are supplemental and may not be required
for every study.

-------

-------
           81-6  Dermal  Sensitization  in the Guinea Pig


                GUIDANCE FOR SUMMARIZING  STUDIES


1. The form of pesticide tested, e.g., solid,  liquid, percent AI
   in technical, end-use product, etc.
2. State if material is corrosive or has pH <2 or >11.5.
3. State specific method utilized
4. Complete description of specific method
5. Reference for the specific method employed
6. Note adherence of the protocol to that in the reference for
   the specific method utilized
7. State the positive control tested
8. Significance of changes from Acceptance Criteria

-------

-------
   _
 3.*
 8.*
               81-7 Acute Neurotoxicity in the Hen

                       ACCEPTANCE CRITERIA

Does your study meet the following acceptance criteria?

 1.	 Study performed on an organophosphate cholinesterase
        inhibiting compound.
        Technical form of the active ingredient tested.
        Positive control utilized.
        Species utilized, domestic laying hen 8-14 months of age,
        Dosing oral by gavage or capsule (dermal or inhalation
        may be used).
        An acute oral LD is determined.
        Dose tested equal to an acute oral LD or a limit test of
        5000 mg/kg.
        Dosed animals may be protected with atropine and/or 2-
        PAM.
        Sufficient test animals so that at least 6 survive.
        Negative (vehicle) control group of at least 6 hens
        Positive control of at least 4 hens, (if used)
        Test dose repeated if no signs of delayed neurotoxicity
        observed by 21 days after dosing.
        Observation period 21 days after each dose.
        Individual daily observations.
        Individual body weights.
        Individual necropsy not required.
        Histopathology performed on all animals.  Tissue to be
        fixed in sin preferably using whole animal perfusion
        techniques.  At least three sections of each of the
         following tissues:
            _brain, including medulla oblongata
            "spinal cord; upper cervical, mid-thoracic and
            "lumbro-sacral regions
             tibial nerve; proximal regions and branches
             sciatic nerve
Criteria marked with a * are supplemental and may not be required
for every study.

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                  ATTACHMENT F
LIST OF ALL REGISTRANTS SENT THIS DATA CALL-IN NOTICE

-------

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-------
            ATTACHMENT G
COST SHARE AND DATA COMPENSATION FORMS

-------

-------
    &EPA
                         Unittcf States Environmental Protection  Agency
                                     Washington, DC 10460
                            CERTIFICATION OF OFFER TO  COST
                         SHARE IN THE  DEVELOPMENT OF  DATA
Form Approved

OHt No.

Appnvsl EipIrM 12-11 -tj
 Public reporting burden for this collection of information is tstlmattd to avtragf 15 minutM ptr response, including
 time for rtvitwing instructions, starching existing data sourcts, gathsring and maintaining the data needed, and
 oomplttlng and rtvJtwing thf collection of information. Sand commtnts regarding the burden tstimatt or any other
 aspect of this collection of information, including suggestions for reducing this burden, to Chief. Information Policy
 Branch. PM-223, U.S. Environmental Protectbn Agency, 401 M St., S.W.. Washington, DC 20460; and to the Office
 of Management and Budget. Paperwork Reduction Project (20704106). Washington, DC 20503.

 Please flu la blanks below.
Company Nwnt
Product N»»e

EPA Reg. No.
 I Certify that:

 My company is willing to develop and submit the data required by EPA under the authority of the Federal
 Insecticide, Fungicide and Rodentieide Act (FIFRA), if necessary. However, my company would prefer to
 tnttr into an agreement with ont or more registrants to davelop jointly or share in the cost of developing
My firm has offered in writing to anter into such an agreement.  That offer was Irrevocable and included an
offer to be bound by arbitration decision under section 3(c)(2)(B)(Hl) of FIFRA if final agreement on ail
terms could not be reached otherwise. This offer was made to the following firm(s) on the following
date(s):
  Nam* •( Plrn(«>
                                                                          tot* •! Oftar
IcerttythailamcUyautnorizedtorecmertthec                                          havemadeon
this term and al attachments therein are true, accurate, and complete, (acknowledge ttiat any knowingly false or
misleading statement may be punfrhibie by fine or impflsonmem or bom pno^r applicable law.
IsigMtur*
N«IM Ml*
•t Company'* Aulli»rii«e' R»pr»»tnlati««
TlUt (Pteaat Ty»* er
DIM
MM)
Ef»A
        II7042

-------
v-
tf-

-------
United Statts Environmental Prottctlon Agency
•A BMBiBm m Washington, DC 20460
^fi"3f l""lMF\ CERTIFICATION WITH RESPECT TO
^^ fcii M * DATA COMPENSATION REQUIREMENTS
I Public reporting burden for this collection of information is estimated to average 15 m
time for raviawing instructions, starching existing data courcas, gathering and mainta
oomplating and raviawing tha ooflaction of information. Sand oommants ragarding tN
aspad of this collection of information, including suggestions for radudng this burden
Bunch, PM-223, U.S. invlronmantaJ Protection Agency, 401 M St.. S.W., Washingto
of Managamant and Budget, Paperwork Reduction Project (2070-0106), Washington
Please fin in blanks Mow.
Ceapeny Na«e
Produce Name
form Approved
OVB No. M70-01P8
Approval fipltM 12-1142
mutes per response, including
ning the data needed, and
i burden estimate or any other
, to Chief, information Policy
n. DC 20460; and to the Office
, DC 20503.

EPA
Reg. No.
1 Certify thai:
    For each study cited in support of registration or registration under the Federal Insecticide, Fungicide and
    Rodenticide Act (FIFRA) that is an exclusive use study, I am the original data submitter, or I have obtained the
    written permission of the original data submitter to cite that study.

    That for each study died in support of registration or ^registration under FIFRA that is NOT an exclusive use
    study, I am tha original data submitter, or I have obtained the written permission ot the original data submitter, or I
    have notified in writing the oompany(ies) that submitted data I have cited and have offered to: (a)  Pay
    compensation for those data in accordance wtth sections 3(c)(i)(D) and 3(c)(2)(D) of FIFRA; and (b) Commence
    negotiation to determine which data are subject to the compensation requirement of FIFRA and the amount ot
    compensation due, I any. The companies I have notified are:
    I ]  The companies who have submitted the studies fisted on the back of this form or attached
        shafts, or indicated on tha attached •Requirement! Status and Registrants' Response Form.'

3.  Thai I have previously compfledwth section 3 and 3(c)(2)(D).
Signature
Oete
NMM an* Title (Pleeee Type er MM)
    (er* H70-I1  (4-10)

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4












i
I US Environmental Protection Agency Registration Standard lor:
1 " Washington. DC 20460
Lj>EPA Product Specific
IT Data Report
Registration
Guideline No.
Sec. 156.120
Product
Chemistry
81-1
81-2 (z)
«1-2(M
82-1
62-2
62-3
63-2
83-3
63-«
63-5
634
63-7
6M '
63-9
63-10
63-11
^ 63-12
W 63-13
63-14
63-19
63-16
63-17
63-18
63-19
63-20
63-21
Sec. 158.135
Toxicology
81-1
81-2
81-3
81-4
81-3
81-6
Nam* of Test

Identity of Ingredients
Statement of composition
Discussion of formation of ingredients
Preliminary analysis
Certification of limits
Analytical methods for enforcement limits
Color
Physical state
Odor
Melting point
Boiling point
Density, bulh-dtntity, or specific gravity
Solubility
Vapor pressure
Dissociation constant
Octanol/water partition coefficient
pH
Stability
Oxidizing/reducing reaction
Ftammability
Explodability
Storage stability
Viscosity
Miseibiiity
Corrosion Characteristics
Dielectric breakdown voltage

Acute oral toiitity, rat
Acute dermal toxiettv. rabbit /rae/g.pig
Acute inhalation toxibty, rat
Primary eye irrttattoft. rabbit
'rim^ry derma! {filiation
Dermal senarttsatfon
Testing not
required for my
product listed
above
{ONK*M/QW)

















.
















EPA Registration Numoer
I am complying, with
Data Requirements bv •
Citing MR ID No.


































Submitting Data
(Mxchta)
(Ctoc* CMlow)


































form Approved
OMB #2070-0057
Expires 11-30-89
(For EPA Use
Only)
Accession
numbers
assigned


































Certification
I certify that the statements i have made on this form and all attachments thereto are
true, accurate, and complete. I acknowledge that any knowingly false or misleading
statement may be punishable by fine or imprisonment or both under applicable law.
»
[Typed Name and Tin*
Signature
•
Data
EPA Form 89*   (Rev. 5-M) Previous edition is obsolete.

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