Final Report
To The
•
Environmental Protection Agency
Title of Project:
Complement Fixation, Hemagglutination-
Inhibition, and Trypsin Inhibitory Capacity
Testing of Human Sera,
For The Period:
March 2, 1971 to March 1, 1972
Project Director:
James K. Noel, Ph.D.
Name of Institution:
Flow Laboratories, Inc.
1710 Chapman Avenue
Rockville, Maryland 20852
Contract Number:
68 - 02 - 0032
Supported By:
Environmental Protection Agency
Air Pollution Control Office
Research Triangle Park, North Carolina 27711
February 2, 1972
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I.
OVERALL OBJECTIVES
"
To carry out 3,000 complement fixation and hemagglutina-
tion-inhibition tests on human sera as supplied by the Environmental
Protection Agency. Of these 3,000 sera, 2,000 are to be tested for trypsin
inhibitory capacity.
II.
WORK COMPLETED
A.
Hemaqglu tina tion - Inhibition
. .
1.
Procedures
The test wa s performed according to the procedure as
set forth in Flow Laboratories' Proposal #30231 an<;l dated October 26, 1970.
In essence, the proper dilution (4 units) of each of four antigens (Flu A-2/Japan;
Flu A-2/Hong Kong/68; Flu B/Maryland and Flu B/Massachusetts) was determined
by the hemagglutination (HA) test prior to each hemagglutination-inhibition
(HAl) test. The test was carried out in microliter "V" plates (Cooke Engineering
Co.) using PBS as diluent and 0.4% fresh chicken erythrocytes. Serial two-
fold antigen dilutions v'vere made in 0.025 ml arnounLs;the test ::;haken and
allowed to incubate at room temperature until the cells settled. The titration
end-point was considered as the last well showing complete agglutination.
This dilution, ,,,,hich represented one unit of hemagglutination, wa s divided by
4 in order to obtain the 'dilution that,would be equivalent to 4 HA units for use
in the HAl tes t (refer tci Table 1).
Prior to carrying out the HAl test, all sera were treated to
remove non-specific inhibitors. The procedure employed was as follows:
To each volume of serum was added four volumes of RDE (Receptor Destroying
Enzyme at 128 units per ml) and incubated overnight at 37°C (waterbath).
Three volumes of 2. 5% sodium citra te were then added and the mixtures further
incubated at 56°C for 30 minutes. Two volumes of PBS were added to yield
a final 1 :10 dilution of treated serum.
The HAItest was performed by preparing two-fold dilutions
of each serum from 1:10 to 1: 128 0 in O. 025 ml amounts. To each serum
dilution was added 0.025 ml of virus suspension containing 4 HA' units. The
test was incubated at room temperature for 30 minutes after which was added
0.05 ml of 0.4% chicken erythrocytes. The test was further incub,ated crt
room temperature until the erythrocytes settled. The antibody titer was
considered as the reciprocal of the highest dilution of serum which completely
inhibited agglutination.
-------
2
Appropriate controls were included which are indicated
in Table 2. A know positive and negative control serum was included in
each HAl test series.
To determine the exact units of antigen employed in
the HAl test, a back-titration of each antigen was carried out. This control'
was performed as an HA test employing the diluted antigen (4 units) as the
initial virus dilution. The antigen was further diluted two-fold for fi ve
dilutions.
2.
Results
A total of 3, 170 serum samples were each tested with
four Flu antigens. In all tests performed,. exactly 4 units of each antigen
were used and serum controls were satisfactory.
All HAl results are presented in the following tables.
These figures represent the reciprocal of the highest serum dilution which
completely inhibited agglutination.
B.
- Complement Fixation
1.
Procedure
The complement fixa tion (C F) tes t was performed according
to the procedure outlined in Flow Laboratories I Proposal #30231 and dated
October 26, 1970, utilizing Influenza Group A and B soluble antigens. In
essence, 4 units of antigen were added to graded dilutions of serum (previously
heat inactivated at 56°C for 30 min utes) and incuba ted at 4°C overnight with
1. 8 units of complement (SV-S free). Sensitized sheep red blood cells were
added and the plates incubated at 37°C for one hour. The plates were shaken
every ten minutes for the first thirty minutes of incubation. The titer was taken
as the reciprocal of the highest dilution of serum giving at least a 3+ fixation
on a 0 - 4 scale with 4+ being no hemolysis of the indica tor sys tern. .
J\ppropriate specificity controls were included in each test.
These controls included: bac k-titra tion of antigen to confirm unitage employed;
serum anti-complement control; complement titration; known positive and
nega tive serum controls; RBC control.
:
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TABLE 1
HA Tes t Procedure (Microtiter TechniqLB)
1
2
3
WELL 41=
4
5
6
7
8
PBS
o. 025
0.025
0.025
0.025
0.025
0.025
0.025
Antigen
@ 1:10
PBS
o. 05 O. 025 O. 025 O. 02.5 0.025 0.025 O. 025 *
L----/ ~~ '~..7l \.-----'/'7 '-...../1 \--~;; 7 --l
. I
0.025 0.025 0.025 0.025 '.!J O. 025
o. 025
0.025
0.025
O. 4% Chick.
RBC's
O. 05 O. 05 O. 05 O. 05 0.05 0.05 0.05 0.05
1:10 1 :20 1 :40 1 :80 1 :160 1 :320 1 :640 RBC
Control
* Discard 0.025 ml
Dilution
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TABLE 2
HAl Tf~st Procedure
WELL #
-- .-.- '-.'---
1
2
0.025
3'
0.025
4
0.025
5 6
0.025 O. 025
7
O. 025
8 9
0.025 0.025
10
0.025
11
0.025'
PBS
Treated Serum
@ 1:10
o. 0l~70. 02l~~ 025" -:12; 02\_7J 025\~02Z ;;J' 02~,. }}. 027*0.025
4 Units
of Alltigen
0.025
0.025
0.025
0.025
O. 025
O. 025
O. 025
O. 025
0.025
Incubate at room temperature for 30 minutes
0.4% Chick
RBG's 0.05 0.05 0.05 0.05 0.05 0.05 O. 05 O. 05 0.05 0.05 O.OS
Serum Dilutions -1:10 1 :20 1 :40 1 :BO 1:16'0" 1 :320 1:640 1: 12 80 Serum An tig en RBC
Control Control Can trol
*
Discard O. 025 ml
-------
-----.----..--'-- -
..
3
2.
Results
A total of 3, 063 serum specimens were tested. All
CF results are presented together with the HAl results in the following
tables. Those specimen numbers which have a (-) indicates insufficient
material to carry out the test.
~
. C.
Trypsin Inhibitory Capacity
1.
Procedure
The test wa s performed according to a modified procedure
of Hummel (Can. J. Biochem. Physiol., ll: 1393, ,1959) as stated in Flow
Laboratories I Proposal #30231 arid dated October 26, 1970. Ba sically,
the reaction mixture contained 2.5)1g trypsin and 12.5).1g soybean trypsin
inhibitor or 25 pI of a 1 :10 dilution of serum sample to be tested to which
was added 0.5 ml dermatube TAME (Worthington Biochemical Corp.) giving
a final volume of O. 55 mI.
A series of 12 tubes, 2 controls and 10 serum samples
were prepared as a group. Trypsin in the presence and absence of inhibitors
were incubated for 10 minutes at room temperature. TAME was added, mixed
and immediately transf~rred to a cuv.ette and the adsorbancy at 247 IVI}! recorded
employing an ISCO Model 610 recorder attached to a Coleman Model 46
spectrophotometer. The rate of hydrolysis of TAME was defined as 100% and
the trypsin inhibitory capacity was calculated by the formula shown below.
Trypsin Inhibitory Capacity
=
(Ra te) Trypsin - (Rate) Inhibitor
(Ra te) Trypsin
x
100
Soybean trypsin inhibitor was included in each group of tests which served
as a measure of the function of the system.
2.
Results
All results are presented along with the HAl and CF
results in the fOllowing tables. A total of 2, 034 tests were carried out.
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-. .
,-
, HAI Titers CF Titers Tryp sin
~cimen Flu A-2 Flu A-2' Flu B Flu B . Inhibitory
Date Specimen # Ta pan Hona Kona Md. Mass. . Flu A fluB Capacitv.
.
7- /o-f.:,CJ '-ft./-O{)q~ I ;LBo /"0' (Plio ~'fO Jt) /0
II - e'l h (P'lo 10 h-n Bo /0 /0 e{O
..
If - no, 121/0 /;).~t'J Ro ~O d--O ;).0 .~y
II -/RO f10 10 1-10 fJo /0 10
" - o'fs . &-=/J '/0. 1:280 /60 /0 10 19
,. - /O.,s- I-fo /0 2?-o 3'2.0 J() 10 If?'
" - Obl./ : 3.20 ~o. Po ?o d.-O 10 t?
" -oP~ ?o /0 10 d-O 10 /0 ~~
It - ()(;,O I J.B 0 / :J.AO 160 I (;.0 ,~D d-.l) '710
II -068 3.;!o /0 .21J ~D 10 /0 I;:),
,. - Odft.J to lfo .3 ;J.. f) 3010 I~O /0 /0 I ..-~
I. - () 71./ 1:< B n 1 l-d) 1 cJ. P, () &''10 10 .;ztJ .;J. ;l..
If -- tJ (.,J;- 100 /D :10 ;10 /0 /0 ?'O
i; . - /t.jL~. I,., V. t] 1/1 ;2{) ~O /0 /0 5~
Ii - 1..3 '1" /&0 ;;20 fv'lo Ilo ItJ. 10 '7b
It - {J,2.~ ifo 10 &LfO (~ 'I- 0 J/J /0 5/
It -01:,1.. ..3.2 /) ,c:P-o 3~D C7'fO d-.O 10 l?~
I. "-/3D ~r:r 0 /D ,.2' D ,;2 0 . 10 ..tt../o ..J4
'/ -&79 ?o 10 if{) ,FIJ /0 10 ;2.
1/ - tJ,.5-, ':? ~~o ID . ,3..).0 Po /0 <10 .~-~
I. - 0.,] ~ ~/) /0 J.() ¥o /0 10 .'3 g
II - (}q I 3'd. 0 10 ~o /60 Iv Iv G:>g'"
II - 0:28 / :l 20 tv 'I- 0 ,4.7- 0 3;;"'D /0 10 ,r;- I
,. -:- 0 I.fj. . PO /0 cPo I bo '+0 ~!J 30
" - ().f7 .b Yo FD rF-o 160 /0 10 ;;2 /j
I, - d. 9/)- ~O 10 t.f I} ~o /0 q,O I (p
1/ - ()~ ~ i;;. eo /;J. p,i) /.;J.£n lieD ;2..0 ,21!J Ire
i{ - O'io 40 /0 ifo ?o dlo .;to . , \
~
It - {) '-1'1 /.J.. fJo I;l. AD Jdflo ~:J.) IJ ~() 10 40
It - 02> 'I :10 10 '-If) .;1.o /0 It) .:3 If.
It -.3'/b ((I Yo I dJ.B 0 ~O 10 d..O 10 ~<) i
[ .
I
[-.
I"
I"
-------
e. .
. '..:.
. '
-
HAl Titers C F Titers Tryp sin
3!cimen "Flu A-2 Flu A-2' fluB Flu B Inhibitory
Date Specimen 4\: Ta oan Hona Kana Md. Mass. Flu A FluB Caoacitv,
7-/0-/09 " .
'ifOJd1i 40 10 . -0 40 rPlJ ~tJ' ..;2.0 iO
II - .23 I (~40 /60 (~ if () 1&0 10 1 0
II - 3- //., 0 Lfi> '/0 3~o rF1J /0' 1+0 44
., -3'1,? J bD /0 i.;J., R- f) 3~1J ,:).0 ~o c.-.!'
I ( - 3Lf9 1;2?o / t,.() .3d. 0 ,~ .;;.. () ;),,0 10 47
il -_~D7 /2AD 1./0 .3;1 0 ??o ().o 10 YO
~I - .-1 D,-j",-' t?o ,:;J. D 40 I~o It) 10 44
II -.3'/1 ,P-o,' ::1 1\ /..f() 41) 'If} /0 '-'6
I I -.33 q 2.;2D /0 cPo ¥tJ JO 10 ?t;).
'I -3''10 /00 ;;J.{) /~O
-------
I -=-""" ~ "'--.--:=""'-==-~-'-~
,',...==.= ..L'. _..~,=-:l--.=,
-- "
-''';';''';''0.-...0--.-
-.."
-- -:- -.. ..
. '.'
i
!...
. . " HAl Titers CF Titers Trypsin
.
~iinen Flu A-2 Flu A~2" Flu B FluB Inhibitory
Date Specimen -it Ta pan- Hona Konq Md. Mass. Flu A 'Flu' B' Capacitv'
7-/0 - 'CJ J..f if () 3~3. ~ ?O ;;;10 ?O ?O ~o ,..!In fur
II - 3--S-1 /bD /0 4.2 0 3:lv" /0 ;J.O (,5
It - -3 J\'O ) L~ 11 .~() ""'Po Zfo ;;1.D dO ,.;zo
7- /1- to'1 SSO.3 y: t./ ;;).o "10 ?O ;1.o ;0 10 ?If "
II - ;l. ~~ /foo 10 3;)0 /0 In 10 ft,lf
u -[) 94/" ¥rJ ?O ~O i.l-o 10 J /) " ;J.J?
II .-:J.cr7 cPO :21) tPlJ Lf-o /0 10 " &7
I( - tJ.s-.3 to YO /,;{!3D 3~/) Ibo (<:('0 ~O [,0
" - .-~tHJ . Lfo ,:If' ~o d() In. -ID S4
.
II - I).p} ~o dlO Po 10 /0 /0 ~o
I I - ;;2 77 .20 cPO PO ;;2.0 ,.;zv ~o
'I - I).I\" I . 3";1 0 3;}.-{j "10 10 :<0 "2n 10
-
! It - t..t '7 if /t.n PO 1&0 t../-o .~v ~D 3
- . - v
II - '-fon 40 .;20 ?o cPO /0 /0 " o-
il -.JbCf 40 rPo ;20 " ..
.;).0 /0 10 '73
I( -~,t...-J <:po. 'd--IJ £fo /0 ::20 d;<() ,
,/ - 0 ~Cf a:-LJ /0 /60 ;;'0 ::10 ~/D ~.!)
'I -330 ?D ~D ?v iD -.:2 n ,;).[) ff'f
If - 3--1- l, /;1. aD :ItJ ?o Lfo )./0 ID
'i - IJ~? ?o " ;1.0 /00 ¥iJ JO /0 -.1
" - 07'? '-10 10 tFO 10 10 10 (~'-I
" - {) '1.2 ~o " It) "10 10 /O ,.;?o 3~
I, - (/ 9/?" gv /(;;0 cPo 10. " ;). 0 <10 . 70
It -O¥iI ,;1.0 < (D 1../0 ./0 10 ;<'0 4;).
" - :J. err? ~ cPo Alo ~o /0 /D 3'1
-30/ (PYO /fro .
It 0 'Ii) Po /0 10 33
Ii -/30 10 <. ID tv '10 ?O <-/0 d.O ICf
.,
It - /:If) Po. 10 3.).0 cPo. 10 J../O .1)"7
. It -/l£~ if 0 10 ;:J.. 0 It) /0 10 .' 1-fq
,
...c
r".,
-------
.--...--. -,~;,..~,-,-,------------,_._J_--:;..~
, ._-~-~ ~ .. '.' ,..~...,._~-..;;...........-.~-::--
..----.,l1li.--.;;:
. "
"
, "
- ,
HAl Ti t ers CF Titers Tryp sin
.;!cimen ' ' , Flu A- 2 ' Flu A-2 Flu B Flu B" ' Inhibitory .'
Date 80ecimen # Ta oan Hona Kona Md. Mass. Flu A FluB Caoucitv'
1- JLI- ~ 'I II 0 I ~.S"" t, I.f 0 1./0 ' c?o 10 ~o ~../O
-/07 ft) 10 /~O 10 10 10 -
,.
II -/'il} 40 10 t '-1'0 3.2.0 L.,f) 10
It --102 ?O /0 L.fo 10 /0 10
Ii - / 'If?' 12£0 I :l!30 //"O 1..J() ;).0 <::';0
1/ -II/ i:2 80 / /"0 kLlo' ;1.0 ,.;). 0 /0
1/ -/3/ ;'0 iO (p I.f 0 PtJ LIV LIO
It -I/O /;;. BO IR L/- 0 3:10 10 ;).0 <'/0
II -tJ/7 .J..o /0 40 If) 10 <. 10
/( -/3:2 1../-0 10 Po ¥o /0 /0
II -lIb liPO :;'0 Jfo /0 ;2.0 /0
It -/0/ /ldJ 10 L.Jo 10 ~'O /0
, 1 - iP9 '3';'0 d-.O ¥o 10 /0 /0
'
" -!19" J I.,., .;10 :Po 10 LIV 10
. vv
It -/~7 ifo ,20 ,J..O 10 /0 ;<0
II - l'f'b '/1.:0 ::lO /0 /0 117 10 ~D
~I -0/9 3020 ;;'0 cPo rFlJ 10 ifo
'
,. -/)3-6- ~o /0 /0 10 Z/O 10
I( -- (J ,1"h 3.;J() ;Lo Fo ~() 10 10
II - ()3b ;3'.;2 [) 10 It) If) //) 10
,
'-t
-------
1-
--....-.
.o' ;
.
: '
"
I~-
I' HAl Titers . . CF Titers Tryp sin
~cimen 'Flu A-2 Flu A-2' FluB Flu B Inhibitory
Date Specimen # Taoan Hona Kona Md. Mass. . Flu A Flu B CaoacitY.
7-1L/-1a'l '10 .
//(){) '10 /0 10 10 - I t..fo' d.D PO ;2o <. 1'0 10
ii -oLf7 ?o 10 (;; 'Ii) PO iO ;9,0
Ii -O~6 '-10 ;;1..0 Lfo ID Jf> 10
I( -O.j.3 Lfo ;;z.o ~D i.f1J /0 /0
Ii - tJ..a J "10 '-10 160 i..fo ;0 l{}
I( - (JIb /bo ?o 1./-0 It) ~O ;(O
Ii -/(;,9 I/o!) It} I..fo 10 ;<0 10
.
I 1/ /60 Po /60 ¥o
-O~D .20 .t!. I 0
I( - O() 9 cPO '-1-0 Po :ID /0 .20
Ie ~ I ?"6 ~.kJ
-------
. .'
-- -=:'-.- :
.
~
HAl Titers CF Titers Tryp sin
;cimen 'Flu A-2 Flu A-2' Flu B Flu B . Inhibitory
Date Soecimen * Ta oan Honq Konq Md. Mass. Flu A Flu B Caoacitv.
7-/L/- lu9 I/o I~;).. ?O .
Ii) .;Lo 10 JO 10
" - ;;..s;;... / fp.{) . J.ff) d.-O /0 AD 10
11 -/60 It; '10 Po J>O LID JIJ 10
Ii - ;1.,;). 0 . / d- FJD 1;;2.&0 /.2 80 /;J..!50' 10 Oto
11 -;) '-I D Po . ,;J. 0 40 10 ~ ID 10
it - d-6<- I (PO' "d-O iP tfo ,,;1- 0 liJ 10
f( - () 3.J. 1.2 IJ() ;;zR /) (p 'I;') /60 .;zo 8"0
It - .::2 9g' ;;'0 ;;"'0 /60 cPo If) 10
Ii - I..'l {); d-O /0 ?o /-f1J ;:)0 :20
It -Dlcr ~.2n ?o to l/n . :3 c;..o /D t.f-()
It - CJ ~.L..- (0'10 Llo '1-0 '-10 10 ..( (0
,( - ()o.s- ?o /0 J>{) cPt) .J!IO ,20
I( -;l'lt> IbO /() ¥t" .;Lp ..1 (). 10
It -.). '-')..~ 3~o to t./D <10 10 dO Iv
-
It . -O{;)'f 3~o 10 10 /0 10 10
II - d l.f;J.. Ibo ;;"0 ~o it) II} /0
I( - tJ3/J /;),80 /:; t.fo 2..0 lD J/-o iO
It - 1/,6- 32D 100 ifo ~() ,;1.0 10
Ii -;),,6/ In 40 IbO I btJ 3~D 10 10
It - ;; if"! ifo 10 10 /0 10 /0
i( - ;).3 A- /00 /0 .;20 ;2.{) 10
-------
~-
, HAl Tit ers CF Titers Tryp sin
~cimen 'Flu A-2 Flu A-2' Flu B Flu B . Inhibitory
va te Specimen # Ta oan Heno Keno Md. Mass. Flu A Flu B Caoucitv.
1,4D .
7-IX--b q Ifi.fO 11. 0 . J:l Bo 1::2 .¥'o 4-0 L Iv ;1.0
. II -- 3/ h' / ;). 8'D. I~R-O 1:280 40 ID ;;"0
It - 010 / 0t.fO 40 3~o ;1.0 :2.0 ::J..()
II -/)0 tv40 '-10 40 10 I () /D
-Il~ " 1;1.&0 (p LID ;;2.0
I( 10 ~D ID
11 -/~;l i..fo 10 /t;{). /~D < to .?o / ~ lIt) ijo /0 /;J. 0 10
II - /..!J-ct liP 0 It:. 0 /0
-------
---...-
.. .~
. ,
'.' "
"
1-
[.--
I
oJ- HAl Titers' CF Titers Tryp sin
" , Inhibitory
3cimen . Flu A-2 Flu A-2' "Flu B Flu B
Date Soecimen # Taoan Hona Kona Md. Mass. Flu A Flu B Capacity
7 - /---')-:"l9 i.f'lO/:Lb' ?0 ,.20 lIP,) dD .
I;;. cfo ~/f)
II -() l/~ i;).. :f-o / ;-' / ;J.. c?o /ltJ ;1.0 /0 ~o 10.
If -;lbLf ,pO d).o 1-2jt-() 3.J.() ..20 ' .2 ()
it - /1./2 tv t..j 0 ?o (p t..j () .3~D J D /0.
Ii -/5'7 ?o :;21> cfo ~() 10 2n
it -/'/3 160 '-10 /0 ,' PD ~l) ?o PO f() 10
Ii - /
I, - .:::L? 3 I ;lJZ-o 4/0 1.2?tJ 3.;2.0 . /0 10 2b
.
It - ::; 7, /~o d-{) /ht) 160
-------
. .
- ,
-----
. '.
[
I
[--
HAl Titers CF Titers Trypsin
.;;cimen 'Flu A-2 Flu A-2' Flu B Flu B . Inhibitory
Date 80ecimen # Ta oan Hona Kona Md. Mass. Flu A FlLi B Caoacitv
.
-7-I.~-- l q ~ IO;}. '-I.. ~- ~.)D '10 &,L/o Llo ID ID itf
-..1l.."- !?/J ~f) '. 41
II 10 10 iO /0
II - ~ 0/3 /tvo (p '-f 0 ./0 /0 ~D /D (." . ~
II - ;l 73 (py.o to l/o 3~D ibD 10 . Iv f?fo
It - I;J.. I,.. 3~D ,;lD cPO Llo If) L./O
11 -,3 'lei '(PY-O . I..f 0 PO cP1J. If,) I'D l'J ~
II .' - .J3~~ /0/) P-o '-10 ;20 ID ~ {O . '7~
II . -'fo~ 1;Z t'ro ;20 J>o yfJ In 10 (,.h
I, "- / Od.. 3~o 10 /(.1) '-10 1 l) ID.
It -/'-/:1 . 3;;"'D d-O ~.;to ~2-D itO' ;20 110
II -373 ?O d-.O rP-o JO ID ID
II -3 CJb . lid) PO (p 1./0 ~~O Iv IV
I( -11.3 .3;>'0 'f0' I L.f) . cPo . ID /0
.-
II - 0 19. / .;( ~)--o 3c-~ 0 /00 '-f() . /:7 ;;'0
-
II - O?.3' /&0 liPO ~o t.JO JD /0
II - .3 ",;2.. '/ .2 ?o / ;J.?O 6 VtJ '-10 /0 .~ D
It - 6J :l.A. 3~o .'dl 0 Llo '10 If) ItJ
/( - -=t;l'; I (PI) .?O /60 dO ~ /l) 10
Ii - IJ '10 tv <+0 '-10 /(.,0
-------
I.~
,-
HAl Titers C F Titers Tryp sin.
,
.. ,.;cimen Flu A- 2 Flu A-2 Flu B Flu B . Inhibitory
Date 80ecimen # Taoan Hona Kona Md. Mass. Flu A Flu' B Caoucitv .
.
,- Ie, - ~9' 330377 .1.2 0 ;;l () . /0 10 10 <10 ~3
" - 36f3 40 <-It) ;;to 10 10 iD 75
'I - 3R..3 /60 10 .'~o ¥tJ <-IV 10 (pI
It -/c/3 3;{o I/;;/) 3:1.0 ?f) ,;J..1J 10 ~3
It -3~7 c?o <'JO L..fn ;1o lD /0 ..3b
If -.3'7~- /.;l 80 .3~o /0 I() In /0 ..71
It -30/0 J>o ~o b t..f o .Po ;)..0 ~o ~~')
" . -1'10 ~LfO liP/) /0 10 -JD <10 of
It ~/3c? /.;i 8'0 £9t../O £./0 d-~ 1+0 ~o. if3
'I -/7;;". d-O <"0 to Yo rFo <10 IV '18"
If - OS?::, lion ,3 ;2. {) 3.l0 ¥l) ;;La 10 iO 10 4-9
h '-10 cf7) "
-- Olu.. <,- <10 ~o ~'V <'/0 ~3
It - ();J..s- ~tJ .;;'0 3.;ln j"tJ Jf) /0 4CJ
II - O:J.-I 1../0 ,..2 () ao /0 /0 10 sr.:
It -I?~ ij.-lj d-.D /bO I.{-' i) LIO It) 47
I( - 1{,{2q /hO '-10 0l.ID I ;( ~o II) i i}
it - '!fPL! ?o 10 :l1J ;;;'/') II.') ;0 ~53
It - ...~ 7 ;;l tPO ?t) j ~r¥t) &'Ia 10 iO S7
11 -3 9<~- cPo .~ to (-10 3.;l{) ~/O It) ~0
- -- -.
I( --/d'l ?o ~o (P ¥iJ 3-<.0 . . ii' ;)..0 i./.-?
It -3J2/ /~o Ibo Lfo ::J..o /0 10 ~~')
It "- L.f71./ '-Iv' .:l{) Ifo cFo /0 10 7!?
- 'i7? (p l./ 0 //;;10 ;0 10 . '7.S-
;t (P 1./0 ~2.l>
I-t - 'i .:2. b b
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HAl Titers CF Titers Trypsin
~cimen ,Flu A-2 Flu A-2. FluB FluB Inhibitory
Jate Specimen # Taoan . Hona Kana Md. Mass. Flu A FluB ' Capacity
7-/(.,-f:Jq 3~noqo' 040 (p '10 ,;;Lv /0 ~o :1D -~f)' 3rt
It -,]9.3 llo() d.f) /~go ;";O lCJ <10 '7/
II -OYS- , &'-10 3;20 ~llO 410 . 4-0 t/O
(,7 ..
Il -OLfta / ;J. S1J ~.;to &L/o '-10 ;0 ~()
It -/p/ ?o 10 Ift-;o d--() 1D' /D 37-1-
/( -373 tvY-o / .:< Y t:J 1.:J.Po /0. 40 ~/V 3q
h -If'") R-o dO / ('0" 8-V JO /0
II -IS'-I /60 ?o cPo 67) 10 iD 45
II -/~;).. C~Yo ;2..0 ;1-0 10 ItJ /0
I( - 1'I'f ?O ;lo /0 /0 If) /1)
II - lift? /IaD 10 eft; 1./:0 .
-------
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. HAl Titers C F Titers Trypsin
""-lCimen . Flu A-2 Flu A-2 - FluB Flu B Inhibitory
ate Soecimen #- Ta oaa Hona Kona Md. Mass. Flu A Flu B- Caoacitv
~:'/~-l> q 33 D /Cf/1 Ro 10 /0 10 II) 10 .
" - / ?,f" t'u '10 J.?-o /IJ,O 'fv 10 ~/O --
'I - {J i./7 /&IJ <"'10 ).0 /0 10 ;1.0
II - /5"';). 1.2~ &Cfo 1../0 d..() If) Ie)
II - 13S- b '-ID '10 1;( f2-0 4;)0 IrJ ItJ
Ii -(7S I (PO . - J.-o 11o{) l~() /D /0
Ii - 'I?6 I ",' '-10 I../o ;)() LIt) /0
It -073 i~.Pv i~rO /hl'J It,:; /0 10
It -1/)..5"" frO g:v (.;. Lf D ID I.{ U /0 ;;'0
It -0'/1/ &VO d--D ~2-0 <.-3"'2-0 - /1)- 10
It -391 / bIJ d.-O L./O ;;"O <. 10 <-/0
It - ('73 ./ d.. 0>-0 > /~?o ffD ;;J.V /0 ;to
Ii -ltPt-y - 3.;) /"') if) dD /0 Jf) 10
II -/6-9 3:.'2.0 ;20 &YD 3::21) ID IIJ
.
If - {) 3.1> d.--O /0 10 If) <. tV 10
I{ - () /.3 11;;0 d-{) 010 10 r2D /0
Ii - I '-Ill 4;tO 10 10 /0 10 If)
i( -.3 '1b i .2rP-o i :LR-o L.fr) 'Ii) /0 /1)
It -/97 0 c..f 0 I ~tPo cPo ~o /0 10
Ii -/;{o / [PO ~j) '+1) <:::.. to <10
i-r' ~ f)::<-~ ~O 'fo '-10 d.-O In 40
" -otf.~ 3d-o' .3.;to t.f() ~{) c:2n IlJ
. -/(,1 YiJ 'fD
11 1../0 ~o It) 10
II - 0;).8" ~D ;;'0 / ;).c?o fo t/o 10 .;3 ,:;t D
it - tJlJ>- ~o /0 ;)0 ;).0 10
.
II - I .3 C).. (p
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. HAl Titers CF Titers Tryp sin
~cimen .Flu A-2 Flu A-2. Flu B Flu B . Inhibitory
..Ja te Soecimen ;if Taoan Hana Kana Md. Mass. Flu A Flu B Caoucitv
I-Il-t~? 330/)29 In Lt () / ~O~ /0 ;;'0 ,A{) ~1) .
.11 -1?9 II.~ () ,:)j) / ;J?o IPt./O II) L..(O
It - /q,,: RO i.?O '?O PO /0 10
JI - 1131 3;). 0 1ft) (PYo 3~o /0 10
II -007 IbO d..-f) ;;"0 10 /0 lG70
" -/'/ / 1100 . ~O ?o. Lfo 10 /.l.J'o 0Vo hJ ¥o ;20
I' - 1.// ? 1..fo 10 j .2 r?o ft, Y. () -< 10 10
Ii . - L.f..2f? -It,) ;l...o .;2D d-.O 10 ;;1,.0
It -- 'I:~ (J Ji-.r> ~/J 1&'0 !PtJ /0 /b
Ii - '-1;1../ /;<~?/) rPl}. IG;,O ~D < If) <10
Jf -'-/tJ? ItPO ¥o 3~o 160 in ID
. It - '11<..1) (P 'If> 10 t.j D 3.;Jo /&0 ID fro
Ii -'/0'1 /:2?O bi.../O 1,,25'D (p l.f 0 . d- ()' ?D
It -.:L// 1;2 t?O I~ ;);)0 ;";'0 It) It) I
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, HAl Titers CF Titers . Tryp sin
~cimen . Flu A-2 Flu A-2, Flu B FlLi B Inhibitory
Date SDecimen # Ta Dan Hona Kona Md. Mass. Flu A Flu B CapacitY
7-;"-10 q 3 ~ D 4d,\- ?O '-If) ?o d.-O to 10 . .
II -~/3 I..f() /0 /(;1) 40 2:'D /0
It - 'It)/) /~o iPtf.-o .3~o 40 /D ~'O
11 --. ils'l I .1 .~n IulfO ?o rPO 10 II)
'7-/7'- ~ r Lf i..f 0 Il.f 3 3.20. . 10 4D 10 10 ~tD
It - n,l q' 3,),0 1 (p 0 3.2n ~ ,2.D 10,
Ii - DL./O ; .'?)~o 40 3'2-0 3 "2-() '+0 .:20 t~ q
:
II -/77 .3;20 ,?-. D I bu' /0 /0 I{}
Ii - /...1,'-/ 3.).0 /0 1../0 ~o 10 jO j-.3 "
II - [J I..f~- I .28-0 3,~D '10 < 10 10 <. (O . -'7 /,
If -/9D ..3;i 0 40 1.../0 rO dZ./) 10
-
If dL1D.334 ' ILl) ;J.. 0 . 'o ,/0 -< ID to
If c-. 3 ~s- 3,,:2. D 10 l: 40 ~ L./o ~o
.
't - L( ~3 b l/o /60 ?o ?t> ~ ID' <'(D
il -~<73 1.2?o hYo /61) /fo - Jt> /0
II -ooL( /btJ '10 cPt) crt) J'V /()
': 1 ij
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. HAI Titers CF Titers Tryp sin
'?cimen . Flu A-2 Flu A-2. Flu B FluB Inhibitory
.Jate Soec imen # Tao:.m Honq Konq Md. Mass. Flu A Flu R Caoacitv
7- /7-(P&j ,;;1. /0/ t?,R' . 3.2 0 ifa ?b PD .
" -t).1".1 ItJo ;;"'0 d-O ;)-O -'
.., -3.3? I :2 R-o .)..0 '?o i..fo ;;'0 10
II - 007 -~ _1 0 4n ~O :;:'0 .:lo 10
" --31n3 ?O _;;20 d-D t.?O
7-/tfJ-1o 9 S'SD IS'.J.,. ?o d-O
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HAl Titers C F Titers Trypsin
!cimen 'Flu A- 2 Flu A-2' Flu B Flu B . Inhibitory
Da te Soecimen :It Ta O.3n- Hona Kana Md. Mass. Flu A Flu B Caoacitv .
. .
. 7- J ?-~ q ~-"I)O 1/ q / .2. tf'f} '> I ~8'V ~O' .:::20 ,-20 10 73
. " - 33-3 &, i.f 0 ;)[) l~o ;;L{) ~() 10 ~:J..
II - ~1-0 J ;< r?D In 10 " jry /0
I ,
Ii - ;J..'?7 /00 10 .;I..{) It) 10 ;;20
" - ;;l ,,>- J I~ D c:LtJ ro .;ltJ 10 ~/O X'c"l
It - J.. 71.f (PYo ;60 /0D .3;ZD :?J.O 40
it --;U?.3 J td) ;J.IJ £fV '10 it} lD
1;. -;)'7? h Yo 3.;1..0 10
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'?cimen .Flu A-2 Flu A-2, Flu B Flu B Inhibitory
Jate Soecimen # Taoan Rona Kona Md. Mass. Flu A Flu B Caoucitv
7-li- 109 .s: 'VO:2 7 b' 3;;10 /0 3~ )6D IV .
10
It -t)74 f.:- f./ () ?/) .3;; f) 'Po ~o iD
II - d- 1(., /hO J./o R-o ;;'o 10 10
Ii -;:),/;,7 / ;1 t?o 1.2J>"O /0 3~o /6v ,;;lA) /e) ID 10
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7-;L..J. ~0q Ij~ O/~;l / ;2 rtJ >1;2.rfo /0 10 /0 10 "7 l~
Il - 0:3 if' ~--10 .;20 i ..;J..F(,J 3. 2-0 ' /0 /0 7,S"
Ii - '-177 4'0 dO - 9
It - 6)11 / d,rPD GYu ~ 10 d.o 10 ..l) J
tl -/~/ I~J{) / ~ tf7) cFo db ??lJ '+() (PC:?
/1 - tJ t?o .3;2l) 3.?to ?O iff) /0 10 . ~-:j-
Ii -&31 IbD ItJ , '-/ (J 'd2~
II - () ¥{) 3.JD 3;;l.<) ~c2D .rv It) /0
If - 0'19 Fa /D ~~b .;Ii) 10 10 '(P {I
I
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17
-------
. .
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. HAl Titers CF Titers Trypsin
~cimen .Flu A-2 Flu A-2. Flu B Flu B Inhibitory
.Jate 8Decimen -# Ta Dan Honq Konq Md. Mass. Flu A FluB CaDacitv
'-.n-/p 9 .J.:l. 01/ b fr; '-10 . ;;'0
ro '. 3;).{) rPO ~o :~q
.It -.;... tJ,f S. 1£0 i./~ 3.2.0 i..fo 10 :J..o 310.
-t)3~. #0 dLtJ '~ 10 ;)0 -
'I lD ?--~
j,( -003 cfi? ~..o IIoD /60 40 Ifo 75"
- 00 if' 3~) 4D t.fo : ~f)
It. ;Lo /0 10
I ( -- 3so I .J. cf-O . i :2<2- D /0 ID J() ,2,O ./'of 'I'
." II - 3~3' /00 d-t> ?O ¥() /0 10
It -0'07 / ,;JfJo bYtJ tf() ?-D 10 <-I D Cfo
11. - SDJ.. 3JD ~D .3 2l) i..f{) .~o 10 ~-I)
Ii -S;}f../ bVo i-fo Llo "10 ;,,) . /0 If?'
II -30h 3.J6 'fD 'fi) ;M) /0 /0 '73
It - 3 ~~- /Go /~u /'bo ;;At) In /0 ,<\~~
It - 3./)'8 /60 '/0 r7L L:.. I D ~/{) '77
It -S-~3 I;;. cPD I 0 J;LO 4D /0 /f) 47
I, '- .s-;l. 7 / ;( J'v otv /(,tJ ~() d.O ~i) I/?
It -SJh ~.;2() if ~ ~O ID ;)..D /0 '(p 9
i( -,-1)19' Ibo ;)..l) 4/J .;ltJ ..20 /{J 5-If
it ~ ,,>-,/;).. (;YD b lfo ;1..0 ;;)..v /0
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I C',ec'imen . HAl Titers . CF Titers Tryp sin
, Flu A-2 Flu A-2 . Flu B FluB Inhibitory
--'ate Soecimen # Ta oan Honq Konq Md. Mass. Flu A Flu B Caoacitv
7-i;)'-~9 ;;2;:;. f) S"'.-~/) , ?O ;).0 dIo iO LID 10 .~r~
'I -- ~-~:?'/ 3.:Lo /0 ~/D ZID :lo /D .~q
Ii - J,7) D /bu ..J..D ¥eJ "10 fa ID 7 (p.
Ii. -SO? .3~o ;1.0 ?D ;;Lo ~O .20
II -DS6 3,Jo 4lJ <-ID 10 ) V '.f7) I d)cfiJ', b Yo PJ' 1;-0 /.fO 0,~
-
i1 - 6);Jl.{ 1(;,0 40 /0 ICY /0 10 47
;'1 - [)?b I ;;2.?D / ;)JV !;;7ft I;? BD S>1J. /0 '7~
h - tJ6 I 3dO JfeJ OL /0 ;
-------
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~cimen .Flu A-2 Flu A-2. Flu B Flu B . Inhibitory
jate 8Decimen # Ta Dan Hena Kena Md. Mass.. Flu A . fluB CaDacitv
'I" .
7-::1;) - l. 9 ~.2 0 1;l. CJ .2 O. Iv 10 In 10 1>-0'
. .., I --':I;l~- to YD 3;lo 40 .;10 LtO ;}.O
Ii - ()37 "II) :J..() l.J cPO rP I..( () 01.0 ().O f.~ q .
Ii - lY.!>b 160 Po ,.1.f) ..:kJ :lo .::10 S-b
" -//;2..' / ~ifo /60 .;20 CJ.O ~D /0 3h
It - '-f 7b / ,./.. Po . 0 YO 10. ;;1.0 10 /0 . 0,~
,~/(. -'173' 1:2 rPo 1;2.PtJ F-o /fa c;l.() d1.o rug
'II -~'II) 3~D dD
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ecimen ,Flu A-2 Flu A-2. Flu B FluB Inhibitory
j :-Ja te Soecimen #- Taoan Hona Kona Md. Mass. Flu A FluB Caoacitv'
7.-,);) - G 9 ;;;.. ;}. O,J. q 3 . ~O 10 /rbtJ ;).t) In /0 t)"r
Ii - 4 '19 dJo 70 )() /'D ~ ~/D 10 S-S-' .
'1-;;l~-hq J.IO/aR /;;80 t?'I- 0 /..to .;2o .2fJ I 73'
~o
11 -- 099 /.2cfV. (r, l{ 0 1;2 .fO fs;, '-I 0 ,..:2 D .::J.() .~b'
Ii -12~ (0 '-10 40 <-Ii) <-ID ;;2. () ;;2.D ~0
if .:.....-. I 7 ..~_. / JUt? /:2;1) .:r..D 10 I 1.10 10. 4?
Ii - (x?> 7J It. 0 ItPO ~o 10 ' Ii) L./O i
I
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It -0;).., I;). Pi) /,J..S>- /) ~ dP ~(()
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: t?9
It -lib I~p cPo In i. Ii? ..;z rj
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I.. - 0 7'1. FP ;Lo I..j/) '/0 I 10 I'? too
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Iv ifo I
'e - 3.?.:s i;tPD 10 )f) i 10 ~ ~.,
d<() .::>
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II 10 10. I /0
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1/ - '3R '10 40 dO dO I 11-0 .2..0 '71
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'i - '11[1 /00 4() ;J-o 10 !
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HAl Titers CF Titers. Ttyp sin.
~cimen 'Flu A-2 Flu A-2 - Flu B Flu B Inhibitoty
.Ja te - 8Decimen ;/:I: Tanan- Hona Kona Md. Mass. Flu A Flu B- CaDacitv
'7 -cJ.~ -Ie 9 .
3JDI31n /~tJ '-/tJ /.;lfo dD /0 /0" if ~~ . -
- ,( ~ '11 to t, \f-o - 3:20. i ;.z.P-t LlIJ ID :~ 0
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ecimen ,FluA-2 Flu A-2, Flu B Flu B ' Inhibitory
Jate SDecimen # Ta Dan Reno Keno Md. Mass. Flu A Flu B CaD<3citv
7-;z:a -109 .3/0/fv:2 / ;lJ!-o 'Yo' ;;"0 <. It> /),0 10 ~5'
It - /.:!J-8 IP /0 iO
" -/99' Lfo -<-tD
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. HAl Titers C F Titers . Tryp sin
- '. Flu A-2 . Inhibitory
ecimen .Flu A-2 Flu B F hi B
Jate 80ecimen '* Taoan Honq Konq Md. Mass. Flu A FluB Caoaci tv
!'f-,J"v-109 iftfO/[)7 . (P tfo /60 t,VD .):JD 10 10 if V .
'I co- 1J.f:~ ?o d-O ItJ 10 LID 10 (P3
I' . -:J RL/ ?-o cJ ./hD 3.,,20 /(;,0 l() 1-/0 ~? II
I( - osz Ir'vD . c:2(i ~;;"D 0=0' :J ()' ,::) IJ (Ph
Ii - Oblf. 302() d~ .3 2.-0 .3 2..() ,J.,O If.-o :1~
" -07b '-10 /0 cPo i.fJ )0 10 t;j
'c - 0 J.~ 1 bo cPo /G:.tJ /60 dO ,:).. D ..5"01.
II -;2,1),2 .3;2.0 :J..D '-10 'Iv :10 ~/O
LI - () Plf 4D 10 IbO .3.;lQ do ;)0 ;:zq
I( - 0 b.5" /~O d-tJ 1" ;;>0 ;).0 10
I( - :l 99 /60 .;10 J"--o /tJ:,D /0 d-O
I( -;lCf? I;;' 91.> i ;;:LSliJ 40 ~() . ;.Jo 10
I( -;ZtJ8 /40 b'lO 10' ~ "'(J 10
.
~ --.,2<93 .3.;2 0 Lfo Lft) / "0 . / 0 10
-
I ~ - :J.8-:l I(;D dlo /60 RJ dlo ' Ii)
-
" - .291./ 3~o cPtJ cPo ~tJ 10 Ii)
It - .39;). 3J.D /6D eft; ?o I-.J-O iO .(., If
-: ..1 S'
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I
I
,.~ e~imen HAl Titers CF Titers . Trypsin
. Flu A-2 Flu A-2 Flu B Flu B . Inhibitory
Jate Specimen # Ta oan Hona Kona Md. Mass. Flu A Flu B. Capacity
7-:i'4-b9 44D.39D /bo eft) cPD 10. /0 ,50
(I -/I~ (;; Va /60 Po ?/J ,-;2 D If)
I ( - 'l3J-- cPo /<.2?o Lio 10 -2 fi L../O 111
II --- '/.31 . I:2Po /2PV J20 ;;to Iii /0 -rot"
1.( -I?~ '-/0 LIO /0 10 If) L./D d.:}..
II -373 c:Lo ~/f) 10 10 i /J -
-------
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. HAl Titers CF Titers Tryp sin
'1cimen .Flu A-2 Flu A-2. Flu B Flu B . Inhibitory
Jate Sciecimen # Ta oan Hona Kona Md. Mass. Flu A Flu B Caoucitv
7-;JS-lc. 9 S,£;od'I."J' '-fa .;to '10 ID ~o 10 reO"
1( - tJ t.f J.. ~ LID' Il'D LfD 10 <,f) -
II - o.3l> cPo d-lJ '?D /~ 10 d.O . .R'O"
" -/0/ ;2() ;20 t-7J J-fo 10 10 :J.t~
I, - 0,31{ rf?D ~D 40 ~/cJ 40 10 (Pro
Il c- 0,,-'2 ~() . &='0 J=o J-o 10 10 ~. q
It -ISh 10 dJo i../o 10 10 IV '7 /..t
it -/S9 <::"1 D I D ?o 10 10 If) . 7~
It - 37,~ Lf (.~ cPo ,RJ < It> ,;to 10
II -/bY L../o J-o .,;J..() 10 ;l..o ~o 74-
it -/74 2:20 3.:L() 1../0 10 'fO ;<() '-~ "7
tt -!0"d- 10 ;)..{) IbD ?o 10 /0 :;;1
1/ --/6/ b '-It) 3.J.-D' /fc,() dO '1-0 ;2.0 ,., ;).
It -13-0 40 po' / Ill) 16fJ if) 10 48"
II -lJJr. c2tJ .~D t?o 70 '- /0. 10 (po
. .
" -/7/' . /t~v /60 3';;0 H; d-.o .;zo .~ q
1/. - (.;lS J60 dO ?o ;2.o ,'J-. 0 ..2 () &7
It -fRo 4() '/0 3.).o .2tJ /fV ;2,O
If - /7 c:; ?.I yo /60 /60 /0 /0 10 ,5-0
II -/.3 Lf d-D 10 .3.).D "ID /0 //) >?7
It --1c.f9 'fo /0 4'u Iv 10 <10 /..[;0
I( -/6'-/ d-f) /0 !Yo d-/) '"' 10 10 ;<8'
I( -/SI./ /bo .20 tYo J.tJ /0 10 ~b
1/ - 2> crY- 4fu ;2-0 Yo /0 If) /0 ~
I( -/6>3 ./ /"0 /0 .;J.- (., .«() 10 Ie) ~~-C)
i, -/S7 / ;2 cPo /~() /I,D 4/D 40 10 &4-
II - O#I.J. /r;;{) 3:;'0 /D
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. '
. ~cimen . .Flu A-2 Flu A-2. Flu B Flu B . Inhibitory
-vate Soecimen # Ta nan Hona Kana Md. Mass. Flu A Flu B Caoacitv
"1- .1.' t;-I,., 9 S-SO~JS.s- /~o /(;.0 10 <::. I D 10 . Lfo 1 0 .c 10 10 . .;- 8'
t I - tJ20 d-{) d-D '-fr) 10 '. /0 10 Lf.::; .
" - ,;Z 94 L(.O 40 I..fb .20 '-.0 ).0 .;;21
'I -l/ ;).. "-0 . d. () ~() iIJ :J(J .::J I) ,1\-,
'Il - :l. II ~o 2t> en> /0 10 dJ..o. &'1
II - ';;S9' 40 21) ~ ;2i) Jf} 10
Ie --- 3d t.f 3~D 3.:Lo i..Ji) 4D /0 /0 ~ff
II - -2M- d--v ;0 3;lD p-o IO. :AD G?O
II - &9 40 .:Lb ~'O '-Iv /0 d.JO Cv q .
II -~,?S- '10 /0 /60 d-o /0 /0 '-~:)..
I( -0)4 po i.fo cPo c:hJ .~D /0 Ljg-
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It -- ;J -~~ i..f~ i.f{) .po Iv ;2D ;)o L..o
I '. b If-o
II -;)t..f? tfb ~tJ <'/D flD ~() ,~7
II --279 ~ It) 3 --10 d.D .20 L[..rJ 76
'I --/9t.f cPo !C> /..j't) //J J 0 H) (;.. (}
(( - .3 (f.3 'Yo 20 3~() '-fD /0 In -1-f-
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II -li~ - Llo 40 '10 10. ,:l 0 10 0q
II. '- OS-I 3,~() 32..0 cFiJ I/J I-i 0 10 "'1'1
(I - /1'-/ /60 :3 L.V J() /0 ,1 () ..:zo 40
If -I If-, .1-/J. :L; l-/o -L() /0 10 3R'
(f - ()P~ /;.-'2 fLo 1.1.?-o 3~ ibO 1f[) /0 . b-,::l
II -- 04/ cPo 16u "fa iO 10 ~o ---t;-?,
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(, - /,19 :2D ~ 3 :Zv ,;1. () 4 1f) .2'O 43
II - as-fj I.fo if) cPo 10 10 ~o 43
/1 -/;L{) 1../0 ;k '-/ [X Ii) 10 /0 48'
II -/:1..'-/ if/) ;J..o 40 10 /0 sr.:
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.Jate Soecimen # Ta nan Hona Kana Md. Mass. Flu A ,Flu B Canacitv
'7- :J ..t; -('J 9 f)SD/.j---~ ;}n . / '0 74-"
lD :M 10 In
'1( -. 1i9 PtJ cPo 3.?-o L..fo ~,o 10 d.&
It ~ 061./ ~O J7J /60 ;201 20 UO "7Q
. . -/11 '-If) ,'k tyO :kJ' Ja . t:(' 7
Ii I 0
"7- ;l.tf- b q /IOJJtJ ;to ItJ ::Jv io Jt) -<-ID J-j,S-
"1'(. -/03 /(.,D '''10 '-10 10 It) /r.? C?!?
Ie -/19 ?tJ i..f'D 40 /0 ..:J.J) /[) 40
II '- f}/ b 4/J 10 Fa ;;>-D In / t.i t, q
I( -- () 30- _Jl'> < IL; 3;?o ::2b ..:2. 6 ' 40 ~3 b
I( -II/ 40 < ID ;)0 it) ~ /0 /D ~4
It . - 03l> /ho 3~ ;10 <-10 i c." /t.)
I( - / P'I- Po ~' i60 ?~. It) It? --. I
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I( . - OS? /bO i.jtJ ?o ,/-o it) !o 7
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~- ~ ?-t}1 I/OL/b~ .
cPo 10 I ;;l?o Ibo /0 :;0 10
1/ -/3R' Lf- () , It) ~o .:l-lJ /0 d-.O 9
It - /1;) ?o /0 160 . 10 /0 10 . qo
It - 070 ;;1.0 /0 rf-D Ie) /0 /0 ,I) I
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t, - 1;) 10 ;;L{) ;;LD ,rD JD L.w 10 ,1)5'
1/ - I~.). '-/0 It> /60 ;).-{) ~ iD IV S~
"f( -07/ ;2.0 10 166 ~O /0 I'D
II -/~-I efo If) d'D "J-() ~/O ID d'1'
t( -I;). 7 ~L.O fo \.fo /6D :20 /0 ~/D !)O
Il - /:J. Y .;J.o If) ;Li> 7D /0 10 43
It - /0;;" '/0 .)0 tvl../D 'I:f(J~ /0 10 " 9
« -1109 ?o Jo ?,o ' .po
Ie - '~7 Ibo I bD :;;"D :;2c)
(.( -(YD. eFt) )0
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1<.. - O,5',"J. ~/) . /0 ~o ;1o /0 10 I q
Ie. -tJL!, 4D ~/o 40 cPD /[} 10 Ib
I, - {)'TS- 4D ;).D / D c:::.: J () /0 i 9
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I. '7;}.73 cPo /" 160 'fD 10 ;;1.0 i c{
II -;).7'b 40 10 :J-o io !(] 10 &4
\ l --'199 3.1 0 bL/o .'32.0 PD
I ( -- 'I CJ7 I tPo 410 j 0() .;10 ..c. 10 <'10 -
I ( -S:2~ (04-0 (0 L{-() 3.)0 'l() ~o <10 '31
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'r - 1.2}.,- /60 /0 ;lD lD ":::"/0 10 37
I( - '-I..3~- PO ~,) lu 10 . d.O' 10 fvtf
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'7-.2)'-10 q /) 0 Dt-j .':Ll f') )D ' c?O ;)D 10 < 1-0 -~ l.F
'II --1).1 /6D ;)D 3:ZD ~2a /0 /0
II -J7J "'-ID ~/) ;;to ?D 70 <10 ~
II - ;;l Lj.5' 1.:lJ10 32-0 4D '~D' ";10 .:20 43
,I, ~ .J.3/7' 3~D ~).o '-ID 10 10 .:I'n 3 I'
I, - ;).. b L( ~() 10 8-D ;Lo 10 /0 :2... z;-
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...Ja te Specimen # Ta oi3n' Hona Kana Md. Mass. Flu A Flu B' Capacity
7- dR-iv Cj J I 0 ,31>8" L.fo ;)D 32D 160 II) IV' ,.,q
It - '-/ /; tJ '10 10 i{PD I..jo If'D Lln (Plf
II - '-I P d.. 4D JD ?O ;lO ) D If) ~
" It -.372 4D iO 4D t./D 10' ,;< 0 3tJ
I( - '-/ YS '-It) , / () 8'0 '10 I D /0 4-;).'
Ir - i.f ;). J LID . ~/O ;}.-D .;20 10 ~()
I( -'f;2? II) '~/D 40 /6D ~ /1) 10 I:J.
I' - t./ q d.. }O <10 /:LPD / ;)(rO ; l) 4-0 .5"7
II -37'-1- (r tJ 4'0 ;;H; ;;Lo 40 ~o
I( .- 3 C; b / {;o /60 crt) 10 ;,20 d-.O 5<-')
II - .3 q.s- ;;1...o ID Lf() ~/O /0 10
I( -37? , /0 Ii> ;;-'0 <'-10 I'D IV 4f5"
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It - i-flo ':rc' /0 ~o d-O JD /0 ,7).:1
It - 3;),7 ifo pO 'fo iO 10 /0 t? q
, (I .-. <~ bia /~,O '/0 yo /0 10 10 -
I( -J02 4() /0 ~O 10 ID .~O cf3
II -"3 L/.~- ..20 10 /~?) /t.:O /0 ...:L'U tvlf
'I -344 / l,o fe, L/ 0 I (PD 40 4-D ::20 -
I ( "- ~ 7'.3~ 4" 4C.. I 0 ID <::"ID , JO 10 ~Io
{i --3?b PO iD /60 ,L/-O /0 /0 5" If
I ( -"J;O? po ID 4D :;2.0 d.-O .:)" 0 ifg
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. a....jCimen ,Flu A-2 Flu A-2. Flu B Flu B . Inhibitory
ate Soecimen ;If Ta oan Hona Kona Md. Mass. Flu A Flu B Caoi3citv
1-;;'9-fo9 ;;L:;.o /2/ &'-10 3.?.o .;;10 /D /0 /0 '-I/?
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.H .3.2-0 10 10
II -IOq '-to ;;to 3-.20 _~
I( -01'1 ;2.~ ;)...{) .3). () ...Po 10 /0 if?!;
It - &'11 ,;20 10 cTO ;21J 10 /0
It - :2 4'1 i:2?o i {;tJ ?o ¥tJ ~O 10
.1 I -OSb ;3:2u /&0 ¥o 4/D ;;"0 /0 7.~
. I ( -09'3 1 ~,O ./0 / tao / be) /D .::In
II . --()t:- '1 f?o / (J ?lJ r?O /0 /0
II - O'/:A Po 26 /0 ~() It) /0
Il -()// 3M Iv' . ~JD /6D ;:)0 1-/-0 (PO
It -O~B J>o ~.o /6D '-10 10 d.O 40.
I t ( -(JP3 32D :3~ / bC .Po. /0 /0 ,0
I( -DOl{ /00 cPv .FD cfD . / t) Iv 7:::1
Ii - ();2 S f?D '?D F() ffD 10 10 4b ,
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I'~ e~im'en HAl Titers CF Titers Tryp sin
..Flu A-2 Flu A-2, Flu B Flu B Inhibitory
. Jate 80ecimen # Ta oan Hono Kono Md. Mass. Flu A Flu B Caoacitv
7-:i Cj-'{o q :?;;J. 0:1 '-I if' ;po 40 j 6tJ &J' :10 10 (00.
1~ -- e,~7 JD ~/D PO ?D LoIO 10
I ( '-/I~ ~o :20 J>D cPO I/) /0
II -097 ' /:J.,.?o /;;A cftJ 0<{o ?D' J.fO 10 y"'-\'
II -- OD;).. 40 iD / (Po Pv J,'? /0 f?f?
I( -1'13 /6 D Po /60 I hi) d-.O ID ,\"I
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II -;)4'3 /q,-/,) 04D 3'1.. 0 ~o /b d.O
I( -/17 ,.po ;;lc / ~ () I bo II) r::ll}
I( - ;2'7'/ 3..:20 i./o 'c?o I be..') /0 /0 'SI.
If --.5'"'0 ~ '10 10 ~-Io I bD In 10
I ( - .6-' :3 ifo ;2..<) , ~o i (;; () 10 ~/O
If - ~ 7(~ 3 2...0 32-0 J 00 5'-0 dlD /0
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I( -'f~1 ~fu ;2D 3w ~ZD /0 10
If -49,) ?o '-!D .3 '2-0 fol{f) It) /0 "
1/ -;),Y3 32-V 4D 32.6 J bD 10 IIJ
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II - l.J!J3" 1.;2 Yo (P'lD Ii) 10. L.. (0 -<10
I' "- 3b8 /bo (P(j0 32.0 ,9() If) S.3
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I( - 50"1 ?D ?D 3:?D 16D ."} 0 ;;'0
I( -;;29/ l..lo
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7-.).?- fufj :;, !J/) /:) 99 ~ . ~o If)
32D /0 40 ~ 1 .
, I ( -:3 b2. &:, Vo /bD IbD po /0 In I q
II -09? ibo Ie) 'd-()' ~ /0 /0 7S
t( -(:3Jt. Jbo ;;2.0 ?lJ I 60 1./-0 In .3J?
I, - 2>-0 I' Po 4D Ibo ;Po 10 ;1.0
It - 'I (/3 /&0 'bL/D FtJ )60 40 LiO
'I,l - tfsS- ' ..Po II) /60 ;2cJ /-to c/O <-S-.C;-
I' -;)./0 J G7D /.Ii) 1.../0 ;2() 10 JD 38'
I( '- 'f ~I 160 -2-t) ?l> ~ /0 :1n ,'1q
1\ ' - Lflb '0 1./0 /60 /6u PI.> ;J-D .J'TI %0
II -1.j5"b 10 /0 ?o t?() If) /0 . .--
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Ii - ;)5'6- .Po 10 t:?iJ FtJ If) In S"?'
Ii -:3.03 tPo 20' ~D ~() , If) It')
-tt -- :J71 4D 'fu :40 If) 10 10 .:?' 3
,t ( - 3 '13 cPD 10 '-/D t..fo' JD /0 ,"-t; "
b -.2In:l- Po I.fD /0 In ' 49
/0 2'~
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7-3>v-bO, 330178 ?o It') 'f~ cPlJ ~11) <.. I tJ ~;J I
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II ~/4) po /() .Po ~c) 10 /0 {yO
, II "-13-7 (p Lf IJ /00 1 ft;.() 100 ..:J.D /2) 70
II '- / S-t:t 3.:Lo /0 i.2c?o 3 2..0 dllJ ~D
I{ -/ ~ Cj '/ fRo /0 3?o /60 ~'If) /0 f:A
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I{ ,-/,P7 /~.Q 3~D 3.?o ?~ 10 ;;0 '/&
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I( -1.2. ~ /00 ID 3~() ' /00 dltJ ,2!)
'I. -/9_~ ,Po :;J.D 4ft) ~() /0 /0 '7 If
(I - /.:'-:? b t./D i?o / {:;D .Po, /0 If} ?-.5
I, - 13,_~ 0 '-Ii) 3;2l) toV-D ~ ~(} ' ,;zt) 00
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)ecimen ,Flu A-2 Flu A-2 Flu B FluB Inhibitory
Jate Soecimen -* Ta oan Rona Kana Md. Mass. Flu A Flu B Caoacitv
'7- 3'0 -1£/1 .3 3n?'f) 1 3J-o 10 cPlJ "10 /lJ 10 -'1~.
\( -/9/ i :2?i) &/fD ~2.0 I iPD /0 ';<0 r-;1o
I( -IJ.{/ :3 it> Ljl) hl./D 1 (;;.1) 10 /0 3'1
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It - / s..!>- . (p i..fD ?IJ (PL/D 32.0 Iv it-D
1/ -.2 S.!:>- 4'0 /0 Po ;Lv /0 i7) .j 8'
Ii -"\ I:J. 7 (P If t[) (p Lf() 4/0 ~ I-fO /0
II --- d. !1Cj' ?D 10 4'[) ;2.() ~O. /0 i-f8"
II -/~ I./-D ID b'-lo Lfi) 10 1-/17 -/0
il -/;),9 320 /0D / (,.J ('" /&D 10 ,.;zD lo 7 .
II -009 3...?~D ~o 3- 2-~ ~~ /0 /t) 8"'J
H - () ,F,;L ?D dJ:; I bD Po II) In 71
. ". .11 - /.?6 /&',0 /00 l../o .;Lo /0 ;2f) .7. c:t
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I -061 /~]o ~o Po J 1) . d..D &'-1
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. - [J I.;: it' ':'/0 ',;1 () / /;0 .J"t). ,F /:;
II /0 /t)
II -113 320 L./o ~/o cPo ~iJ -:2 n 4
I' -097 3- ,,2-D jv ?o 8"0 ;).,D c:::2f) _r8"
" "'- :3 'I J:> .9-0- / (,) 'Ia :J, D IhD ~() Pi) d.D /0 Sy
II -:... .3 y.~;). I;J. J?v I ;}. Yo 3 2..-D JtJ /0 /0 40
It -:.{)/) 40 !tJ /tvD J?o /0 dlJ if:;..
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It --- fJ.!.J-/ 3'D /0 /tao /00 /O ;lb
/0
II .- II tf 1:2.Pf) /0 /60 Po /0 /0 33
I, - 01/3 PD ;';-0 32.b po If) It) ~v.
. \I. -//6 /,2?o / .'2 c?o j (PD c::Po ,..;20 '-10 ~7
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.Flu A-2 Flu A-2 - Flu B FluB Inhibitory
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"1- ?,'f)- In 9 330119 --3 2..D 32D ~o ~D It? in ,~-O'
I, -.;. {) l.j- blfD 40 32-D IIoD d.O ,..2n 7.4-
'I - f) 10 l..f J (PD M }hO ?~ 10 ri..n /?'~
It -- Oh 9 . .-Po 4D Rv !?D /~D ~f) 70
I( - tJ() l/ ,.M ItJ 40 '10 /0 .t./o (pq
II -3"6 &40 . 1 Q JtPD It? /0 ~/O .,2 1.1
u -/0;).. I ..2.Po /~?O It) 10 tfo 10 I~
If - 31vJ- PI> ;>"0 (p Llo cfo /0 /0 {q
II -OR? IbD ;;;lo 3Z-D ~2.v /D 10 ,'->-t.j
(( -:3/'1 ,PD iD Jivo ~ 2.0' /0- 10 . ffS
1/ -- ~ O,--t,,- I 2. 2rD ';)...8-0 bYo b <.(D 40 ;0 (".5
\( - " .PI./- ~{) IlJ 4'0 < it) !f) /0 (n .3
. -If -07? ",2 ~ I~ ?t; ?o - 10 10 70
« -utI;).. 3:1.~ /0 cPo I (,() 10 10 ',0
Ie - /)tb.cS~ 3~o 1/J .'3 2.. c Po /0 10 J 10
II ~ 3 7-'->- 0 "-/J (;; ~o i00 .:Po t.j() 10 -/ If
II - 31":> 40 <10 If;o ?o If) 10 ./:""}
I( -- 273 )t::o Jb 3~ Ii:l') dO /D ~ l>
I( Co. ::2,?;2. cPo .( /D 32.-0 cPo ~O ;)O 40
II - 3-2 3 . ?iJ I" i./l) /00 /0 4./{) {J q
If -;2.39 I (;" () Po 4fiJ :?() /0 ~/o &0
If --09'-1 0Vu' (P I..f D I..:;,,pa 32-6 10 10 31
I( -oD3 I;<.~O I :2,po ibo ~o 1+0 I() fvS
I( - ocr;).. l(;,() 10 /6u /6iJ 10 ifo 10 If) ~'7 .
It --076. /00 ..2., () J>D /6D ~o J(} .? q
«( "- D?:3 Ii) ID ?o t?D 10 /0 ~:> 3
I( -3/6 p-v 4D '-ID ~/D /0
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ecimen .Flu A-2 Flu A-2. Flu B Flu B Inhibitory
-.uate Soecimen # Taoan Hena Kena Md. Mass. Flu A Flu B Caoacitv
~-3~-109 ' .
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Ie -/?9 ~ 10 3.~ j(PD Iv If) . ..,. ~
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(I --/bl ht./D PD lho 32-D' /() ID jo
II -4/;;;. 1 bu ?D ~ 2-0 3 2,.l) 111 10
I( -- / 0~- (p LfD . ::J.. D b lft) ) ~o tfO C),O 'If(
.11 - '-120 1,:;' ?v / ,;2,?-o 3~D 3~o J 0 10 J;'" ("
,/, - '-I~ / lho J to u 320 320 10 ID /4
.1, -D29 L./O /0 &> c..; 0 b 1../ /) 10 /0 I 0
I, - '103 ~20 ;;2..() .32~ 3 2.-.0 . ,~(J. 10 10
I, -It:, 3 ?D ..;J.D ibo ihO 10. 10 .7
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(, - o'/? Po It) JIvD 4D 10 10 '10
Il - (Jot- /~ 9'0 12PD /&0 /0D :J...D 10 /f,r
I( -337 L./v d.E> 40 €'o /0 /0 :.;< t.f
Ii -olJ 9 (pl/o :B 20 ;;"0 /c;,D /lJ /0 'Iff
. t ( - 3 3S- xO /0 ?o 40 /0 10 Ff:t;
1 ( -//0 ~~o /0 . d-.D ID .;2D d)..D
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II . -3?J1 4D iO .,;< 0 /0 /0 /0 '7a
I( --/0,/ J..fD /0 Jf/;D /0 ,;1..0 io 31-;),
II - () 3 2. /620 ,~b ~O ' ~D ~o /0 &0
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7-?;o -to 9 330,-;s ?o ~D :.J.. 0
1\ -.241 )"bO / t,; () FJO J h () in 10 ~7
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?-I-/oq .5"SOf)47' ~/O 0 ~/O 2)..0 0~
I ( - O!o:2 : 310 , (- 3 ;;1. {) Po t?O 10 /0 ,;l. 0 fr7
II -Ob? I tel) /0 10 /0 ..(./tfJ 0
I( ---I tf'.2 /00 119 dO '-10 /0 ;;'0 ''79
II "- 07 b I G,>O /0 dlD ,..2D ,d.,/o .':Z 'l> ?..~
h - D f?1? 100 Iii> g-o dO /0 Lfo 8if
It - () c;g- 8-0 10 10 Jo ;;..0 If} ~t",
1\ -f)73 .3..2D, ?o 110D to Iff) ;;'0 /0 . 0-Y
t, - () 9,~' Jlo '-<'/b <. /0" <'/0 ;).{) 10 47
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9-1-109 .6:1)'0;). / i~ fro ~/O ;J..{J '1-0 20 'l.In ' ''7 .
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II, -:l7R' ~O ;?tI 3,1.0 (PLIo ~n ;:J..o "to R
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/1 - 3'1c'1- 40 <10 :20 ;'-0 7R"
/t '- ~..1':<' ,~:;:.2 0
I( -~~!? 'It) 0 /0 .;1.0 10 10 .:1n /ff
I( '-.;;, 9.6- I;;. gO /.J. g-,1 /0 10 43
., .0:- :::< S ~ /~?O I ;;.?O /0 1./0 ';;0 o=z-n ' tf?.
--
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II '- ,2.2.3 i ?u? /60 10 /D ;20 /0 7'7
I( ~/;;'9 ~O ;:<0 /60 .?-o ~O ,;2o '77 I
Lfo
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~cimen 'Flu A-2 Flu A-2. Flu B Flu B lI)hibitory
..Ja t e Soecimen .:#- Ta oan Hona Konq Md. Mass. Flu A Flu B Capacity
.
f?-/-/~'q S';.5-0 I q -3 40 /0 :lho 3.).7) 10 ',,;{ D ?s-.
" - I ~2 ~ ~D L.ID Llo 10 /0 10 II
'I - I-;J,n 40 10 Ii) 10 10 /0 &?
II - 1'-17 d-O ~o ~/o <::.,0 10 /0 q3
I( -/73 J.:J.O ./"-8'0 10 10 /0 10 ~f!
" - 19 R' ?O '?o 40 '-10 iO ;tfJ /J-j
. ..,( -1,-/9' /lvo b'lO /O ;;"D 2.0 ~O. tu~1)
II --- /,'JO . ~':? ,~o 1.:1. ~o JD Ib ,;{O ;;'0 37
,( - / L. -~ KO ~3 ;) 0 c2D ID ;ZO ~'i) iP3
"< ~ 1 is'' 40 ';;'0 40 /00 /0 ,.1...0 ? r?. .
.
'if -1./ - t:, 9 //0 df)i ,E.JO ,f?D d-O 10 ~'D /0
It --.}, q b 3~o 0l.0 .,;){) 'IV ;l.() 10
I 't -.3/9 gO 40 10 1 () ~D 10 ,
I -- -
II '- 02..5-j:r ;;'0 7 'io I 0 /D /0 /0 /0
Ie '- cJ. ,3 if. ?o /G?U ~O (P'lO ?D 40
I( '- .3;;; s- eo /(;, [) <1'0 ~I/) /1.<0 .~o
II -- 31 Et" 3~o 040 .;10 10 LID If)
I( '-~7,?' ~,72D I{.;O '-/0 /0 /0 itJ
( -.;)"19 /& C1 '-10 ';;'0 ~D I~o /D
I{ '- t)0'1 lfoD ,;20 t.;D .J i-:J. 0 foLlO ;2.0
- -~ L/o ff=o 'lo "
II 40 Ie') -1D ;).D
I, . - 3,r,2 ~()
-------
- --
- .
1- 'e~imen BAI Titers CF Titers Tryp sin
.Flu A-2 Flu A-2. Flu B Flu B Inhibitory
.Jate Specimen # . Ta oan Bono Kono Md. Mass. Flu A Flu B' Caoaci tv
8'-4-&9 liD .2;).!:.- ' I ;1 .~o /t:,o 10 '-10 ifo 10 .
II -39D 1_2~O 3.2 D .J;) ?O fO LfD
tI - -~ tf:5 KO ?D .:2.0 /bV PO /D
Ii - :;"So l../n 8"0 -C...ID
II '-~7t. /60 ~/O 1ft) ~f) i-fO /0
I( - O;)b ~O 3,;2.0 40 10 dO <:/0 l;, .:;2.
II - '173 ~D ;) ej. 10 ji) 40 it? rP'-f
t, - .;2,~-1 .3-,;) 0 10 /0 /0 "::::/0 '7 I .
I()
I -_.
" -- OIl?' . 40 ~ Ie) 2() 10 ~D 40 ~?S
'.' - LfbL/ /(;,0 ID //) JO 10 .;lO ~t-J.
I( -- /,3 I ?o . Lf() /&0 ?o ;2..0 d-.D 71
I I - ()J,-'~- ;:lu .L:.ID 5:>0 ~o /0 dO 7,l)
(I - 'Ib8' d-D ''':'ID L.(u <10 /0 ';;;"0 ,~~3
(\ '- If J l?~ /&0 /0 10 /0 10 /0 .::2.1
'I -- 000 40 . /0 <-/0
-------
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I
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HAl Titers CF Titers Trypsin
~cimen .Flu A-2 Flu A-2. fluB fluB Inhibitory
-vate Soecimen 41: . Ta oan Rona Kona Md. Mass. Flu A fluB CaoCicitv
[f- 4- ~ q J / () fJ 1 -:1. x-'O 1D J D JtJ 10 :20 f.?i'
It - D3 f?' K'{) 10 10 10 .:2. D 10 . 0)-' I
,. - Lf I.r~. 40' <10 10 In If) 10 ?' &40 1(;0 hLfo dO 40 .:).. D t+q
. 'It - 0 ?..!J- . I t" 0 .3,)0 ID 0
I( .- '/J>- b fo4D I ~ 8'0 10 10 ,:).0 c").o ._)<.t.)
It ~~7:2 i;;. /10 3.:1.0 /0 <:"/D O1..D ~O (? .?'
" - oSE? 40' /0 d..U /.f() ;/..o ';
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.Flu A-2 Flu A-2. Flu B FluB Inhibitory
Jate 8Decimen 4:1: ' Ta Dan Hona Kana Md. Mass. Flu A Flu B CaDacitv
~- 4-~q /J 0 I 90 1:JS?O b 1./0 .3-;;1. 0 40 ,d. 0 ';;"0 .:Z~.
II -- ,() 10/ iPt.jo 110 0 ~/O 10 ,AD 10 7;)..
Ie -.JI./R Ib() .3~o
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- ~cimeri .Flu A-2 Flu A-2 - fluB fluB Inhibitory
Jate Specimen # - Taocm- Hona Kana Md. Mass. Flu A Flu B- Capacity
~-S-ib q :l:J. 0 t) q 7" l_J 6'() 3;1.0 .3d.o , ,Y:-O f?tf) ,;2.0 ~S.
1\ -043 RD 4/D /i:,D .:Jf) 10 10 7b
~ c - -()(P 9 4D I 0 40 10 :2.0 10 3D
" -ORb 4D 10 IrD ID 10 /0 S9
I( - 0.1 I 3..?o - 3.20 10 io /.fO .;2.0 ".0
II -OR9 1(00 . tYO 40 go LID d-O tc1.
1\ - Q70 040 "40 40 /0 10 If) 4/
I\. -07:1 d-.O /0 O '7_~
.:.- d.\. ~-(.... ~() 10 1&0 <10 ID- 1&0 7 ,.,
t( . .:::>
I( - I?g' .;J.D <10 I&,o ~D .0::::10 .;2.1) 00
I( -/7b 1(20 10 c?lJ ~1J ,20 ~o 7:S"
II - L/ /1'-1 ?O /0 d-.D /0 If) 10 fv~
I II - '-IDe i ~~O ~O ;2[) /0 2fJ 10 C:;. Q:(
I
II .- 099' / fa 0 4D /D <. 10 10 10 "',-5"
1/ "- o,e;-') ~3~D j~[) 4.ID
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HAl Titers C F Titers Trypsin.
I ~cirnen 'Flu A- 2 Flu A-2. Flu B Flu B Inhibitory
Jate Soecimen * . Ta oan Hona Kana Md. Mass. Flu A Flu B Caoacitv
.
f?-S-Io q .J ~ n '-/7.2 / I;' /) /0 40 ID 10 ;20 ~-~
. I( - Llh d.. ??tJ < 10 PO <'/0 10 4() "3
I( - t-/L/ 7 1ft: 0 <:: I {)
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3cimen 'Flu A-2 Flu A-2' FluB Flu B Inhibitory
-.Jate Specimen # ' Ta pan Honq Konq Md. Mass. Flu A FluB Capacitv.
g--S -fc 9 ,-'2 ~ 0 11..s- .
go 10 40 ~o '+0 ~()
11 - II J- 8'V ~D 10 If) ;).0 10
'1( - 2'7 f? ?O ?,o 10 10 1/-0 If-o
't --/38" po 10 ;;1.0 10 ~v 1./-0
j.( -S;;.O .:2..0 10 10 10 1'0 10
I( - 2LfL/ (p L./ 0 '/~o ;).0 /0 ?O ~D
It - ::2.?4 . / ~.D gO 310 40 40 J>1),
I( -/5/ 40 /0 40 /0 If) dlD
,. - ~"!)-D ;20 <10 /00 ?o LiV 10
I< ~ ). g:2... ~).D .."J. 0 /0 "'-10 ;20 .20
~I - .3 7b eo /00 10 '-
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J .. CF Titers Tryp sin
3cimen . Flu A-2 Flu A-2. Flu B FluB Inhibitory
...)a t e Specimen # ' . Ta oan' Honq Kana Md. Mass. Flu A Flu B' Capacity
tr-S~/c/1 ;)d. D3 7,.1 4D 10 ID ID 10 ~:?.:1 0 9'1-'
II - -~ IJ to ;)..0 <10 I :J?O foLIO .;2.1) /~O C,7
II - 6-.). J i..fD <. Ii) fro .2D 10 d:.0 :?'f
If - S-;:;.. c:; dO ID toLfO 40 I'D 10 ,?L.t
II -~7~ 40 ID d-O /0 I 0 .20 7.?
I( - -~ D:J .3;).D ./ fD 0 jbD ED J..f) 10 4cr
II - .\.:J ~ PO 1(,0 <-10 -
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lcimen Flu A- 2 Flu A-2' Flu B Flu B Inhibitory
Datp. 80ec imen # ' Ta nan Hona Konq ~,1d. Mass. Flu A fluB Caoaci tv '
.
i?' S -In '7 :1::; () II .J Ro ,.10 ID I () . 40 ;to 7S-
'1 I -/7~~ 10 . 10 J'J.O 10 It) 10 ,)"7
I( -:2 bO 1 0 3 S 0 i?b !?V ;<0 ;1.0 «0 II) io 1-/-;)..
(I - lOLl ?D /0 10 ~O in 10 SI
11 '-())i.f. I /,; 0 ;)0 ;2.0 /0 /0 iD .'73
II - tf ;).;;.. ::<. /) :20 3;).0 J~o ,2.D eJ.-o '7Q
I( '- 09 J l?D 6(.(0 '-/0 L./o 10 'J ,) 179
I( - Ifv I I ~1f'O I ,:,< [to 10 "10 ;;2.0 In !?8'
II -:- tJ I{ r? .3':20 'd-D <10
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~cimen .flu A-2 Flu A-2 Flu B Flu B Inhibitory
Jate Soecimen:lt . Ta oan Hona Konq Md. Mass. Flu A Flu B Caoacitv
~-(~-f,., q ?:. 3 D:J.. f) d-. 40 I 0 .8'"0 ID 10 :2n
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~cimen -Flu A- 2 Flu A-2. Flu B Flu B Inhibitory
.Jate Soecimen #. Taoan Hona Kona Md. Mass. Flu A Flu B Caoacitv
. & b'
8"-fp-71 ~~O;)'7() 3-;)V I,,;; 4 0 R-O eo ~{) '+0
'If .- oc?/ I .1 C?D I ,"J~O 10 10 ¥o ;2..0 P3
f' - /;)47 & 'fD 1040 4D 'ftJ 10 10 pC7
1\ -347 . '" t?0 &'fD ;l..D ~/) 10 10 I (~
e, - ,:2.3 q 3~o /&0 ;)..0 '10 10 10 ,-P.Y'
, e -:Jqfo &Vo ' !t.;L!O 10 I.f() 20 ID 0'9
I( -;)47 &40 3~o /D 2.0 10 10 '?I
If -/::In I d. t?o / ;;1. PO ,2.?-0 l(PD /..fa ~o ?J
II -3?7 / :2.J'D iP'fD «D
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"::!cimen .Flu A- 2 Flu A-2. Flu B fluB Inhibitory
..Ja te Soecimen # Ta oem. Hona Kana Md. Mass. Flu A Flu B. Caoaci tv
?-b-&/j 3.30 lJ_~!? /iaO f?O ;20 .;LD .;2.0 4-D '7 4-"
c. -D.,J (PCfO -'3.7 I? -J.O /0 ::20 /0. -7 I
II - DJ"f.o 3J-0 ,..R;1 d.f!) < If) R.fJ :AD ((3
II -007 I/o L.ro ;;2.0 10 10 10 t.? 0
II '- -3/ q 'To J{) <10 L..rO ;z..D ~,v
1/ - 03;). ?tJ 10 ;Zo dJ..() /laO ;;2..0 8"'<1
II '-30_~ $~2 V .r? t:J JlO c:<.o ;;...V /0 ?/
II -/..s-0 .(?O ;).0 10 /0 10 /0 1;t.
It - 01..3>- .;3.J- () ¥o /0 ~(D /00 /0 05'
il ':-/(".:>3 / :.J. ~:.o i ,) /j" 0 .c:. I f) «0 J?V L..IV G~
It - /t:,~ 3-;J.() /~o 10 <./11 ,?. 0 «0 &0
II --.3 ff':;- /,;< ,sf D / ;;. ?o 10 I 0 1-/-0 ,?-v c., ..;-
. . I( - 'fS.s- ?o ;«(J /.{-o ;) j) . /1) 10
j( -3.0l? ;)0 /0 . i-fo ir1 ,~D ;:;Z[) h~__._.
v
I L - .;?v) 10 /0 20 I-fo ;).() 10 ~.-
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It -009 t.'l.{.{) L..ID iO 4/0 .. J..f:- 0 ;;;"0 . ..
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1\ .- 0 t..r; pO ?o iO ;;A 0 LID d-D ID ',7
'-"....
II --IJ 79 Jfc ~2() 10 iO c2D ;o Ct... '0
II - j If Cj 3,J. 0 /0 !?o 40 d-O ?tJ (?~
1\ -0&3 ;;;;'0 ~/D ~/O fJ t.f ;;);;"0 <"10 /0 /0 ;;"0 10 '/8"
52
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~cimen 'Flu A-2 Flu A-2' Flu B Flu B Inhibi tory
.Jate Soecimen:!t Ta nan Hona Kana Md. Mass. Flu A Flu B Caoacitv
.
~-(P-bq _13 V D.".),'?' 3;1.0 (p Lf() It:, 0 ~o 7-fD lD 77'
I( - ;2..,S-_~ / ;1. 6-~O (,;,40 Iv /0 .20 c2v 70
,I - O«.t:;7 ~() If) 40 10 ;1-0 - 40 10 '-10 ~D. ID If) 'II/?
I( -/7'-1 ;l{) <10 10 Iv 10 4-0 (/1 3
10 ..:...- 'I J / 1& 0 <10 I..f() I?o iD ' /0 2/. b'
fbD :
II -~l-:J.. ~o ":::'ID /00 10 J/) ''YO
.. "- ()::27 '+0 ~o ,;l.() 1&,0 10 ;10 '{?s
Ir .-~ (p-"'> 40 ,..2() /0 ~o ).0 40 ..!.J-q
- .-= ( _"'S'
I( - :2D7 L/.() 10 <10 . .0 I~O ~(O <10 :10 ~/{) ,p ~
If '-37/ '-If) /0 tfD ';;'O 10 ;),.0 rrCf
,( -- ();{ 0 8'-0 10 ;;1..0 ' / D /0 10 X 'I-
If -~/3 /&0 10 "-/0' (..10 Iff) L. 10 f{~
II -074 ifo Jf) /00 70 d).D ;;"0 C1' 4-
, II - Lf6"7 to LID 0'-10 ?() /~o 4-0 ;2.0
- --
II --- 3 'f3 Ii? L/. 0 &40 1../0 :2/) , 10 /0 R.3
'I --;?~ tv if 0 ~~.:J. 0 bLiO h 1../ 0 ~o Ifo ~7
53
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HAl Titers CF Titers Trypsin"
cimen Flu A-2 Flu A-2 Flu B fluB Inhibitory
va t e Specimen # Ta pan Honq Konq Md. Mass. Flu A Flu B Capacity
33o:Jta. ~ /fo 0 /oLIO 10 '-I D ;),0 1'1 .
'?-(p- (j q /D 40
II - 3~-1../ 1 rfd) 10 1 (~O PD 40 .2{) -'J.'-
.')::>
40 d.O -< I D )..0 10 .-
II -O/,.}q /0 ('I!J
- '-/.s- d /&0 " "d.f) /~~
II 10 ~o L...IO " 10
It -..J 01 1£, () , f?'O 4. It> 10 20 10 ~ 1
II '- O:J....- ;1.0 '.(10 ~ ID' < ID ID ~/O ~.s-
II -Db? 3;;'0 ~o L-{O <10 ?O :J-{) , .;-'" ~
Ii '- Lf i 7 .3~ 0 /0 /{O ;;1.0 L/.O ;1() ?b'3
If -/?/ t..jO /D IR40 1040 ,)..D ;2n ,g--:J
1\ ~d41 40 1 IJ (PLIO 100 40' /-fO '/ 8'"
II, - I<-J"II / ~ go I J.?O PI? 1../0 1./..() :J..f) (,;, /f
If -_:t 1)1) 3:;'D !oLlD 1-/0 J{O 1.£0 /0 i5?.
, 'II -147 160 10 If) 10 ?D ;(0
ij ,-3(0 /00 4D ~/O -< 10 4-0 '-(0 {? '7'
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Ir - OILl/ ' ?o '0 I;ZO 14;0 ;)0 1CJ
,I - Lfo~ 40 /0 d,{J 0 10
1.( -:J. 7.-:)" 10 Ii) /0 10 ;0 10
I( - Jfv4 tfo ID ~/D
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3cimen 'Flu A-2 Flu A-2' Flu B fluB Inhibitory
.0ate Soecimen -* Tanan . Hona Kona Md. Mass. Flu A FluB Caoacitv
?~7-L q 1f403J/.?' L. (0 &b '
3.;J. 0 . ~f') 10 ~o '/0
(( - f)71 'ft) /D JtJ 10 10 ID ?3
H - /Jt./& /..fo 10 10 -
I{ -~f)(.:> RO ./ttJ ..2.,.} ,;t[) /D in :? t.f'
(f - I) '1.:1 i ;) ,~-=tJ . 3;L() ./n 10 ;2..D /0 7tJ
n - ..J?-o f?o /0 /0
II - ~l.3.s- lro 0 ~o J60 ;;1.0 / ,,') A/o .~.:;-
II ~~.37 /&0 ..
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3cimen .Flu A- 2 Flu A-2, Flu B Flu B Inhibitory
. .Jate Specimen # Ta oan Hona Kana Md. Mass. Flu A Flu B Capacity
?-7-~q 440d2?'1- /~ ~O J:;20 'D If) ltG 11
it - '-f Iff) R'O /0 .:1..0 10 10 10 f"(4
H - -';'/Y7 / In/) g;O 40 1.10 ;).0 ID .;5 0
c t - :193 /1.,(>0 3;;'0 . R'o 40 ~D ;1.0 ..1\1
II -if2& I~ tJ . I (PO 10 10 10 iD ...1)'1
.' IJ - if 0 L/. 1[0 /f) &, Lf () 32.0 .1 [) ;2.0 l~
Ii - I :J.. 0 g>O /0 ~/O «0 4-0 ~D fc,i
II "- 5'1:l 6:>40 c20 ..:J() /0 ~D 10 ~.2-D /'-,0 ~(; iO ,::l D dO q f
,\ - .}?J;- i:)?o /IvD ~o ;;o JfO i() 46
" "- ()b). . t?o 10 1+0 ::2,) 10 .c:..{ [J ~')q
'I( '-.3S I {P40 {fV <"/0 ..?o 10 <10 & I
h -0(,19 /foD I,'",c ) t.., 0 ~;). 0 .;zo ,2 f) ?-2
'I -liS ,10,-/0 3;< tJ b
II .- [) b &- .?O «I) '-10 /0 I() -l-O ?~
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. HAl Titers 'rryp sin
~cimen ,Flu A- 2 Flu A-2. Flu B FluB Inhibitory
.Jate Soecimen:# Taoan Hona Kana Md. Mass. Flu A Flu B Caoacitv
g''-7-109 44-0 Lf~ 7 1-fo 10 . /&tJ 10 10 '+0 q 7'
I( - 437 ~~D ?fJ i-fo /0 JfO ~o ,:5>.:<
,- -~7~ rPt) -10 ;;/.0 /D 10 ).0 (.,;:;..
It -ORo /,~t?O &'10 !?O 40 20 ~o ICJ
" -/7? ''740 3,").. 0 In 0 10 10 /0 d-.0 ;20 7 (.7
II '- 07¥ ,,?D /0 10 If) 10 10 ,;;r .:).
« - Lf~1 ?O 10 /0 /0 ?V 10 ,f'.1)'
I< -- D 0? . /fa /0 ,>?o /0 ,.;( () aD Jf~
L( --, 0.30 /60 :20 1& 0 IreD 10 ..;2. () (, i
II --371 I ,;( R-V 1 ~z /?o i ,') frO 320 10 /0 L. b
If '- D?7 Rv :10 40 ID ..2 0 :30 ,~ ~
II '- / cf ¥ 8'0 10 10 /0 10 ...;2. [) .~-o
II - .3c?:? 10 10 If 0 <'(0 d..D 10 .1S'7.
It -- 'i:l 2> ?O ;).0 ;<0 /0 /0 10 /%
II -- Lf 9 3 Ero ~v
-------
. HAl Titers CF Titers . Trypsin
( ~cirnen Flu A-2 Flu A-2 fluB Flu B Inhibitory
-vate 8Decirnen # Ta Dan Hona Kona Md. Mass. Flu A fluB Capacity
Ft- R-Ioq S:",fJ 3 90 ;;'0
-------
. . HAl Titers C F Titers Trypsin
.
~cimen .Flu A-2 Flu A-2. fluB fluB Inhibitory
-.Ja te 80ecimen # Taoan Hona Kona Md. Mass. Flu A Flu B Caoacitv
r? - <;(-/.0 9 .crn ISif /&0 10 ;;'0 /0 ~O '.~O If(
Ie - .;2d L/ / ;)., 1? D 3;)0 ?O 10 /?D ,.:2() /1
H - () ?Io Ro /0 ,~o .;)o ;10 /0 '1 (I
H - D;)' 1(' LiD 10 4c) /0 ~D ;).O 18
I( -d-..'7;J... 100 ID /0 10 J-fo ~o 7~J.,-
II -IJ,q / {~O . /0 LfD Iv 40 10 lf~
;( -- ~2 .3,_r;- (P'lo jO ...J. D II!) J..I,tJ If) ...., g
CI - 19'1- I:J.?O I ,;J. gO j~'d) /(~ 0 ,;2.0 10 gj
It - ~?'f l,40 t, '-10 10 /0 6>0 10 -In
I( --J~!). 0lfO Ilcc I-fO ;)D ;J,o 10 -, q
1\ -191; .~.;2- (J 40 10 Iv IGD 4-0 !Y4
Ii -- /,;2.:2 /60 /0 /0 I 0 LID 10 '7.~
/( '- /0;) . /~() 3~D" /0 ID ~D 10 .47
£( -- ;;) ;J. c. ?O ;)0 1..f6 d-D J Jfa /D gS-
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I' -.... V~,'I ?O /D /0 10 jf) /0 R.t.,-
It -/;;1.9 ('rO /0 ,2.). U I b j) ;)..D .:20 g,?
(e - ()9~ .3 ,:1 () /~6 /60 ~D /00 ~o ~~
(e - -. 03/?' . 'fa «0 ID
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)cimen 'Flu A-2 Flu A-2 Flu B Flu B Inhibitory
Date Soecimen # Ta nan Hona Kon a Md. Mass. Flu A Flu B Caoacitv
(,? LlO .
g'-I/-bq //02/..,9 PO /bO PO :J..D ' In 7:2'
. "1\ '- OJ.! 7 "J)..o. Ilvo Llo 10 d-O iO 74-
I, - if bb I~?O I ,'?1 ,:':<
,,- - '1193' .;3~o / ;;( ,S?o /bo Itbo ~o 10 f?i.f
It - 07:1 40 ,3;1.0 dO ID to 10 9',')-
c,. - Ifi./S 3,20 3' :}. 0 40 ;;20 1+0 1fO /? :r"
t( ~017 3 .:2. 0 b '-10 3d-D /0 4-0 '+0 q}
,
II - (.)S7 it,D ~O ~:).0 .3 ,A () -;),0 2n P7
h -Dr~ I I.~O d.O Po J.f() ID iD 1-~
I, '-479 040 ;;'0 '-If) cPo /0 10 ,-P.S-
L -Oq; PO Ifo --1'0 cPO 10 10 ?':. ~-
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1\ -:1.6"5 10 Iff} . /i¥O ,~~.J.o """;1 :20 ;;'0 ??/
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I, '- 47'-1- tfo 0 ,20 . ~o- /?o Lfo ?'J
c, -Dol IlvO 3 r-?. 0 It) /0 ,pD 10 -7 .~
II -023 8'0 ?o /~o 100 J() 10 ;'?v
I( -OJ; i-fo 10 Ii. 0 ?o )f) Lfo . .?:<
l( -{)7? I ,:< gO 1;( 8"0 Ifo ;}.o 40 10 ?4
Ie - ¥6-C} ~Lfo / :<. 80 J.JO /0 40 <-/0 cP.5-
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II -32b / ;;<. PO 0'-10 Ifo '?o J./O ~O Cj ~~
I< -;;"90 fa '-10 /&0 ;)..0' I.fo ;)0 /0 ('P7
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~-/1-~9 IIOI/..'J- /ft;O :J..O O ~() 10 IY
I~ - Lf?1 ~;2o J-o ;;1..0 ;.1.0 <10 iD '1.3
'1 --. (l, 03 3<~D 10 /<."1 10 /0 10 ~)q
I( .:.- l,P (;. g"() ::: /.6lJ ~o i("~D 3 >-'0 ;0 go ..5-9
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..)a te Soecimen # Taoan Hona Kona Md. Mass. Flu A Flu B Caoacitv
[?'-/l-b9 110 '-I~3 3";).0 OD '-Iv '-fo /...fO --~o SCf.
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~.: li- foCf ;v...o 44 / I~go 1:28-D . (?D ..;l.o ~() :In 7q
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II - ifo t. &'lO foLIo ;;'0 o d-O 10
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-Yate Specim~n # Ta oan Hona Kana Md. Mass. Flu A Flu B' Caoacitv
'8-- 1;;- to C[ d-.}.O if 0.5'"' .~),o /lJ ':!;:lo /bD ID ;)0' ~- if . . ~
j( -- {)/t 040 /(J {., f../ () ~ if () 10 40
It - () '17 I J.fD ?o b '-10 ~(l;) 1..{D dO LfGf
It.. -037 ?o 2tJ ID 4.ID ,;;. D /0 ~;;.. /~
II - 03 r 3~o /0 l;)Yo I~:(.p () to f l<. 0 4'7
(( - ().!J-b 3:J..o ./60 ;)..() ..;1..0 ,;).D 10 <~q
II -- 0 ~'f 3J-o 4rJ 3~/) cPlJ £'/0 /0 91
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I( -071 /60 IfD /0 10 10 j() 7 ,")
II - :; <./ ,;, 3;2.0 . J.ftJ i;2. Yo i ;;... cfl D. 10 10 (, ,.~
I( -1;).'1 J ;l t;'?~ b '/lJ 160 t.fo :1n 4-0 II
It - ;)../0 l-fiJ Jt!) It) Ie) /0 10
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Jate Soecimen # Tapan Honq Konq Md. Mass. Flu A fluB Capacity
rr-);) - i:.I q .;)..:J..OS'/7 / bo d.() 10 It) :J.() ./D R'~-:-
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II -S37 j bO /0 /0 la ;)0 ,.0 ~)q
(I - :).s;~- 3';-0 ;)..(J 160 ;1..0 \ ;"0 ;20 L,~'
Il -5"'14- ,?o /D 7o !tJ 10 10 .?s-
(( - Lfl)/ 3-.2tJ ' '-ItJ ;).0 io d-U 10 ~'7
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(I - 1/3-7 /).j?-o (0 c/o Po 4D 10 10 '?/C'J
It -.!;-4.5 I l:;() 20 10 10 ;2n 10 90
{( - I..f /7 PO ;).0 :3;;. () J (cy{) /O ;]f) ff"/?
II - '/,20/ ?D Ii) 1./0 '-10 ID 10 c?J
II -,~ c;t~ PO II) 'D 10 llJ /0 ' ?Lf--
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I.r -'-/39 3,Zt) lfo ,)..0 10 10 10 'Jf
II - ;l &.s- ?lJ ~o 4D ;20 ;)0 l-fu P9
I( - 479 /l.fo 3.;1.0 /00 ¥O, 40 10 ?~~
(I. - .s- ;;'.0 I ~J1-D ~o I (:..0 cPD ~D v:l. 0 '{,)-
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(( - 02 '~- / ,,£), L:../D <:/0 ~/i) 10 <: /0 6"....)-
I( - 4fiJy /6iJ ~ /~O <-/0 ~O ;)..O 8>/
I( -379 /60 ;).6 PD .;lD ':<0 ~o -:Ph '
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?- ,-;;. -10'1 ;;2...~.O L/P7 .PO ;2-0 . /,LPt> '-10 /0 ,;). 0 .5- 0/
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II -399 &'-10 10 /0 ~/D tfo 10 ,8'-
II - .a (;,/)- j 6:.,0 . ';)0 10tJ j.fo d-D -'-0 trJ
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'I - 3>~7 04'0 Ie) /(,o ;)..t; /0 10 30
I( - '/00 ?o ;).D io <-/0 ,-2() 10 r?" /?
I( -~3? ~o ;;L6 .c:../o 10 10 ~tO /'1/f'
It - :J (17 I ;2,J'O ~ '10 .3.7-0 ' Po LfO 10 .~.~
II -330 ~;2o i../D /0 /D /0 10 'I,~
It - -:-=3sB I,). tfo cPO /0 /D 4D /0 g:.c;-
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(I - 'I9~ 5'-0 If) 4/0 -:l/J 1+0 ;;"0 ~/
1\ - ~~.-Pv / :2. ,FO /1.c:PD /6D cFO '-10 /0 ?7
I( -b6D Y:D ib it> 10 :LD r:).. 0 '/9
( -OS9 160 Ii) 16 ~ 11-0 :10 '70
I( "-/~o /60 /0 I..fo Llo ;).0 /0 74-
(, -.37:~ ?D /0 ;;'D :20 ij:() 10 ,-P-r'
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It -/b? 3;20 /t) ?D ~tJ ;).0 /0 C:' ?
, I ( -J93 bl.irJ Jl-O L./iJ ;;2.o ;;2D l-.o (?tJ
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It -311 Iho 10 /60 ?" ,;). 0 /0 'f,?,
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(( -.3 7~ .Po 10 /60 ~lo 10 10 too
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_...Jate Soecimen # Taoan Hana Kana Md. Mass. Flu A Flu B Capacity
~-l;). -tog .J-). 0 t.j iLl, 40 Ii) /0 /0 iO ,/0 'i ~ .
I( -1/9 1_1 ~/) Po ?0 ;L{) ~D ;;"O '7S
II - .:2:2:5- 1 (~D /0 ;J-o JD :J.o 10 'i?cr
Ii '- :J.31:. cPo It) 40 2fJ Ii.. 0 :J.D
Ii - J '~:2 . ~ t.fD J"o Po 40 10 fO 'J ,t) ,
t~ . - 307 / ;l. Po . ~c.j() It) ItJ ll2f) 10 'Ij~
It - 3.P3 /60 lu /D <-ID / &0 10 8'S
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t( '-/7'-1 I .2 .PO / 2.P1J ..J-o 10 lv Llo /0 (? i.:- .
II -'~/4 100 ;)..0 04'D PD ,:1 0 10 GO
«( -IV:!:' . bl.{o t:.. / D J.;LD ~tJ ' 1'0 to (;,;:)..
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I< - 3 '10 LID /0 4'D 40 /0 /0 ¥?
L( -t.jD''J 160 . bl..f-o' ..1-0 io ,.;1. (J .t::./l) 5.;<
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t<. - L/66 /60 ;£0 '-fo "II) oZlJ 10 '7.5?
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?-/3-bc/ LID '
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I ( - '-1'-17 L.f() /0 b '-ID (P lIO r10 RO 7;S"
t( -I.!~ -0 10(/0 /6[) '-/0 1./0 /0 ~'IO ~4
If - 0:2 (l ID ~/D ;2D ;;'O If) LID ,3g
t I - ;2./)":'2 '-10 . In /f) 4.:2
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I I - OIl, / b(J /60 t.fo '-It) ,:J() 10 l I
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I, -r;JS3 3:2.0 /60 '-/0 ;).0 Jf) 10 '74
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I( - '-I.] if l{zo 10 3.20 40 I {J ~;2n ~t-,
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It - J oS-- ~.2c> /D It) /D ID ~/O 3 /'1'
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II - .3 ~/3 /00 ;J..() .<.f{) .~t> IIJ '7 /.., .,
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g>-14-~q L/-...'10,;J S I '10 -'O
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-uate Specimen # Ta pan Honq Konq Md. Mass. Flu A fluB Capacity
ff-/~ -L, q ifso O.P/' Lfo /0 ;1.0 /0 10 '/0 f'.~
II - () 77 /tD 10 LID /0 10 'C
1\ '- Df?9 / .:l.?O to t./ 0 -2D 40 --10 40 ~;J.
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f?'-/S-lu9 ..5""'OOOlt).2 '31.0 /0 40 /0 /0 ':1 f) '7;.f
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1\ '- J 64 1&0 Po ,~ ).. 0 '10 J.O LtG ,~-7
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8>-/ iF-t'J 9 / (t') I.."-t, Ita/) /00 /60 10 ;)..0 ' /0 (P b'
I( -/1./3 10 Lto h '10 ..20 /0 :Ao ~o 3.!)
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?-/CJ-~q :;l 0 tP .:3 /) 90 ~/O /0 10 ..c::: 10. J..d-. '-/0
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L( -69~ ?o < ID ifD ~:.Lc> d-O /0 t'1b
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« ---/93 /bD 1./0 L.lt) 10 < 10 .i.tO fv~
t( -:3 9{~ :3...2 0 'l~ I/o ?t1 . ff'{) :1.0 10
.Io( --1/3 3L~-O '-.f () (p £II) :3 ::z () 10 '1-0
I( -- 057 (p £.j 0 /0 160 cf() /D if)
(I --- !,).. -~ / :~ ff-tJ 1.;1 ,¥D '-10 4D J.-.o /0
I( ---IS-g 'fD Ii) iD 10 10' IJ,Po j :JFo i+o ;;"0
I ( -;r9 ~o /D ~o 4D If) 10 '7 ,S-
(( -09.~ !c-:> 1.(0 3- :;.. D <10 <'/D 20. 10 ~q
II -. () () -.)- 3.20 3:2-0 ;20 ID :).n 10 -'J~
l( -/ <~ l./ 3.20 (p 1./0 ;;Lo /() if) 10 4::J
. 'I - 0 (;(:Y' / (? ,<:J ID i.fo t../o 10 iO &~ 3
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. ( -/S"' 3 b (/0 cPo /6D eft:) /0 10 .,,-~
(/ . -//0 ,?O ID 21:20 S>() /0 10 ')->
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I( -- {) I..!>- I~D L..fD /60 /6D ;;La 10 I-Jfj
l/ -1/9 Po iD Po LPO ;;'0 10
I! -: ....'1-0~ /60 ID 3.:zD 0 '-I- D ~o 10
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~-/9-(t?9 ~O ooet. i /PD /0 /t,po /i' 0 ~O ./ 0 .
/. -- O{J) .3 .2. 0 10 ;).0 ;).{) ,. (PL/O 1&>0 ~o 10
I, -37? /bO <-IIJ &;> 4'D 3:::Zo 10 d-J
II -;l8'(;, I b.o ,i () /? t('D ~, ,/ () ~n !f"O
II -,,':J '3 q IGo If) J (,,,fJ ~n . ;2/) !1()
I.. -lOr? l.1.Po 3.:2n 1f) ,;20 eo LID
I( -I Iv '1 3?--n 10 /(;;0 It/) ;)...f) I 0
It ..:- /2 q 3..Z-D /0 I it,'f) 'Iv tfO if{)
I. - /'-17 /t:,O ID / bO /(;,Q ; IV '-to
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,< - /09 I leo jbD', :j,J.D LfO '+D 8()
\I - /,2) /bo <. ID Ii) 10 /0 If)
" <--, /;j?. 1../0 10 :2J) ;;z. f) d,{J iO
i, --,}.. (;, 7 ~lo ID /.2 f-~o > 1,'2 Po /0 ;l...o
It - /:J./ .3....2.. 0 ~:) ;1 D ~o ,;:< () /0 ~:IO
(I - / Pt.. li;;D lb ~ 'If) hLl-O /0 10
I, -//)-1 J.2[) /0 1./0 1-10 iO 10
II - :J. 9 ;}.. /fo ;Lo /D 2D ,;l. () 10
I, - :J.t:,1 / ~2 po . 6 '-I~) Iff.) ~u lD ~ t./o ~t) ifD JfV /tj 10 .i..fb
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qcimen .Flu A-2 Flu A-2, Flu B Flu B Inhibitory
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? -I Cj - (,':/1 :20/ if..)" / ;2,f'o /2g-o ~o ¥D 10 -/0 47'
H - "17 if /bo ID ?~ 40 /0 10 l..-O
Il - £f6g 3-;2.0 (P '-I () 3d-D ibO ID 10 ' ~.'i
I, -l/3 ,?, J ;2.fD 640 /2fZ1) 3J-D 1-?() /fa
II -:2S8 /&0 Jv 3J-o cPo l'D 10
(/. - 3q~-- 3-~o -;20 crt) 40 l-fO /0
I( - Lf;J.() J0 /0 ;J.:)() Eo /0 II) 0""0
I( ~ 3 7.7 - /hO dO 41J /0 /0
,J -J
I( - 2Cl~ .3;20 :2D ./&D I (;.{) ~D If 0
(I -- ~ 10 / bi) /0 6'10 (o'iD 10 ID
(/- ;'J.t1 b q 3.:5 O/'P4 ~~ ,? l) 1 Lf~ I :J.Yo ?o .;2. ~) 10 7?
b {) . L
II - /;25"' .;s ~ l~ b4'o I bD ,;2. 0 ,;zo la Y.3
I( -:1;07 8'0 lbo ) hv J=V iO ~l1
II -/90 Ifo /i~o' (p '-/0 J 00 /0 ::;1. 0 ...£) ~
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~-:)D-(oq 3~ol'72 Llo Jbo . tJ YO /bD . <10 It) 49
/( ---/bLt Sk> 4D 3;).0 b '-ID .3;)0 /60 II) 10 ~57
II "'- I 9/? 3.;20 ,3:2 D 3.;2.0 ;:1 .J.D ";<0 10 Ii'b
II ~~9_~ Jfo 3' ;). () 3~.() .3;2.D Jt) /0 .0- I
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I, - 3..s-:~ .3 J-D I ~iO ~'.,:)-{) .JhD 10 10 t7 i.j
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. . II -/9.5"" , '+0 YO 3~o ;YO Ii) }t;.? .tj}
-/97 Cz!f.{2 b4-o rf'O ~ ' /~? ~- f
It I" <:'-.-) 2.1-0
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I( '-11/ p/) :Po ' ftJ ift) ?O /0 /D ft- ?
\L -/l.J3 (('6> f?tJ i(Pd 4'0 .L.. ({) /t) :?()
II - i;)..o (;. tf () i~~ roc..lo ?tJ 10 3 ;).. () ,?o 1(;,o ??O !u It) .-73
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Il - ,;1..3 '-I I 4,- 0 v:Z {) .iL:2. 0 ?o /1) <'/0 / C:"
I. -3.~7... I~()' 40 0'+0 / ('...0 /0 /(.) .57
" -:147 I?() !.-lo 3.20 ,?o 10 ID ,-~
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II -/()<~- /bo 3~2..o 0 '';0 3..:< 0 lu vZO
Ii. '-3.3'/ to If 0 hf-lo .'3.20 100 /0 ID ,...,..,
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II. -09b 3,;zo IbD /?,;o. .Llo d;'Zo 10 4.?
(.( - =? D;:). J t~f) /(;D .3.2.1) /0D ;:<'f) /() -5 If
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Cv'fD ' ,4 D ~"O
1\ - 3l,;l /~JY) /60 .crD
1\ - ;:) 3.2 .~o '-ItJ !;;;..?o 0LfD ~D ,..2 0
I( -13 L/ lIi) ~D '-If) 40 /D iD ?' S-
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II -055'" )00 rPD /60 c?o Jf) iO ?b
Il - ;:J. ll-:J ~/;.- b 40 ; fl.o
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l( - {} ~1. ::.A ~;).. D /6D j (. () J../o :1n ..;~G ''77
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I, - '-11<5"" /:2 cPD I ;) --y..D )(,D (Po ;;;"'0 10 /)>..1:)
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I( ~ .3Jv.?, I-fo lfO 'fa J~'z> ,,2. 0 .~ 0 7-3
I( -- t.j 61 J?o c?o ?o crD /0 11-0 .54-
II -210 40 ?o 3;)D 1 t:; 0 /D dO 4;;..
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'1( -- 07/ tV-a /-10 ;20 ~tO / OJ iD (.,3
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Ie - {):2.?, .320 ;;'0 b l./- () ?D .c:./D .-21.7 .::) 7
I( '-/79 h'-lo" ,.po 3.2.0 4D i() ,,::l- 0 G 0
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{( -I/O 3.1-D IbD 'f;a 10 10 /D 73
I! - O~Lf. ~:?b LID .3~ A.D /0 .:).. 0 ' (a q
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1/ - 0,)4 IbO 3:2-.0 3}.D 32D /0 ..;< 0 ,,\ J
t( -;),/9 RO '/D 320- to l../'1) 10 G;O '7r;
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X5 -~7::; /. q /-ILJo 3 ~ Cj 0 (-/0 h'lD . Llo J.fi) 10 '10 .
l( ""- {) 3..2' It-D /0 /0 II). ID -~ n "q
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It - O{)t~ I ,2 ,PO I;;U:ZO . 7:2PtJ J :2(?D L. ~D 10 t.? "2
\( -/7l" It,() 2..1) r?. D /0 iO to ,5cf
-«)if 3-' . cp L) I~D 3:10 tfO ,~iJ
tl. . ,p{ D
l( - {} l.fO 3,:)0 '-10 ,-:yo 3:2 D iO ..2 f) . < 1)- q
l( - d.. D::l "pO ;).D ~O ID ID 10 '71
(( - ()03 i../o ;;J..t> 10 Iv ;0 10 ,P;<
If ~ OLfV, i ,) /?D ~o iD /D .~D Ii) -(; :?
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If -OOe/ 10 t..J f) 32-D 2D ID iD lD /73
II -l.2. c'), I .:J. J7- 0 b 1.../ D i0() Z-fo 1..fo ,").. n ~O
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I( -D~<7 . 0lfD /-10 10 /0 iD 10 4-~
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I( -/&,7 RD '-/0 ;;lo .;).o L: 1 2.') /0 4q
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I( -09,') /00 )0 "-10 <10 /D iD 7CJ
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II - ()tf'9' ?o .:2.D ;20 10 10 10 '7.3
Il - ;;)d.-O to '-/0 '.~20 410 ;;2.0 10 /0 -1 S
I, -.2: (~ 9 '-10 10 16D 3?-D ID Iff) 4'7
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I( -/31 32-0 /D 3"2.0 ?-lJ 10 In t;> ,f'
I( - O~~S' '-10 10 ID /0 I 0 iO (oJ q
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-/go b!.fa 2>2-0 40 Jo ; 40 10 '7?'
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t( -/'1;;.. 'fD 10 40 ;).0 /0 0>-0 ~J
L( -113 iPt!o /0 /6u 10() II) 10 5'f
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I( - 0'10' ~ t.-o ID 40 'fD L I!) ItJ ~3
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l( --IS:3 /60 £'0 40 ;;.D /0 ;1.() '70
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l( ,-.;< b 9 / &ID /D i~2?O b l.ft) 10 d:<() ~b
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I( -,)j{ r?o :20 ~D ' ) 60 ¥D -2.n '70
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II -D07 .1;)0 .;20 320 /tvo /D 10 5-3
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II ~ {J t.",'-, 320 ~o L.ID c2C liJ 10 -{/
320 : b 8~
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IL -/9,1 (pljO 10 /D /6 Iv 4.(D (? b
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I( c-. /7b Llo 10 '-10 '+0 /0 /0 74-
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II -,..:)SS 6>0 10 c?o I~D 210 /,,')
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I( -ltJ/ .3~./) In ;;;'0 ..J.. /) 10 10
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<:s-;)'7-(PGj 33 0 O~'>L/ PO .tt::..IO 1
«, -2'/'- I ,;{ L\~O / ~!?O 320 ,?O /0 /0 {?~
,,"
, (, - ..z 99 11PO dO ,20 ID 10 Ji) Y-"7
II "- 00'7 /fpD ?o /0 JD :A() i.::'\ D 'II
I' ..:- 0.3 i.J 3.~o to &=t' ID Jr) ;;),0 {o ~
II -:2.77 ?O
-------
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HAl Titers CF Titers Trypsin
., ~cimen 'Flu A-2 Flu A-2. Flu B Flu B Inhibitory
..)a t e Specimen:\1: Ta pan Honq Konq Md. Mass. Flu A Flu B Capacity ,
.
~- ; 7-1,./1 3300R"3 /~o ;1.0 10 If) 10 '/0 xi'
H --/;>.7 /lvo 1ftJ /:1!?O I Z /rO LfD 4D 7~
It -- Lf3b ?o /D '-Io ,.;),() ~I
It -.,3"/.3- I;;). 8D /(,.0 1-/0 10 dl-o /0 '7~"J
I It -/ rY'() ?o 10 'fo €PC) 10 ~D 56 I
. ~'9
-------
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HAl Titers C F Titers Trypsin
~cimen 'Flu A-2 Flu A-2 Flu B Flu B Inhibitory
..Jate SDecimen * Ta Dan Hona Kana Md. M a S5. Flu A fluB CaDacitv
?-:l7-{,/1 .~30!D:1 PO lfO ' /0 ID 10 " /0 S~
IC - D cIS /IoD 10 10 ID ID ID -
II -lJt~t. 3.-/ 4-0 10 10 I D . 10
.",...0
It -;)73 160 ;)f) ?o ?O /D 10 71
It - Olfcr Iho . ID ;2.0 10 J n 10 (,? q.
II - 1;),3 ~llo . 1/.0 10 ID /0 .:)...0 5-S
H -- J (~ 7 (~I../O '4'0 i I;) D :~.. 0 ~o /0. 8'Q
I( '- ()~,:l ~t) /0 ?O !tJ 410 ;;'0 ;Aq
I( -.3 &-:2 1 ,~ 8'>0 b cfo )0 10 In ,;;. r: 71-f
Ii ~ IJI.i I .:2.0 I C) /0 /0 ID la gy.
, iD
It - O.?5' 3.J. 0 ?O ;20 ./0 ;)..0 '10
-
« - Ljj-h . [f""/ /0 /00 ~() /0 ~o [,:2
I,.
~( - ..22?' 1£;;0 ;;'0 ;;'0 /0 JO /0 ,-/ :J.
« -- <:2; 19 j :J D() (pciO ?O )(;:[) JD .;).. D ~ .~
? "
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II - L/'I& Ib() 10 10 I D jD /0 31
(i -~0~ Po 10 1../0 dlO it) ,;2, n .'
i~ - (2.3/ i d.. ~~o (p I..fD
-------
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HAl Titers CF Titers Tryp sin
, ~cimen Flu A-2 Flu A-2 Flu B fluB Inhibitory
..Ja t e 80ecimen # Ta nan Honer Konq Md. Mass. Flu A fluB Caoaci tv
. .
8'- J.?-fo q J..f t..j,fJ 3 6;...r- FO Llo fr{) ,;2 /) , JI) /0
It... '-3'1/ fPo LfIJ t?h g() 2.D In
" '-10 LJo
(i - ()C?3 L/0 ::10 10 If)
Ie. - 378' J&tJ /60 IftJ lJiJ /{J I{J
" -37S " L/(} 10 I.{.lJ Llo 10 10
I l
I( '- '-I3/S l?o 'd,/) /fs.~O' /6D 10 /0
, It" , -0.6-9 liD C-:J. 0 lk() /btJ ' It) 10
II' - ¥0--5-
-------
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, .
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I c~ ~Cimen HAl Titers C FTiters Tryp sin
.Flu A-2 Flu A-2, Flu B FluB Inhibitory
late 80ecimen # Ta Dan' Hona Kana Md. Mass. Flu A FluB Capacity
~-:)?-(, 9 4 'ff) J-).S" 40 ~D c")D ,"). D iD -< to .
jt -2'13 J../-o /0 ?IJ ~IJ ID -20 -
(. - 0'/"'- ,;20 JD 10 10 I [) '10
(,( - :258' .3.;L() /bO ?O ,;2o .:J..D Ii)
It '-;c;S- go .;2D eD cYD ~/O 4.tD
-
i.. -- .30 I (0 t.f 0 . 3;20 /60 ff-o d.~ ,:J. 0
1.,.
I( - ()7:)" ,.yD qiJ Po 110 dl-D ,..;z () ,
-
£l -- .3/'1- ' (.po ~ It? 0 /60 ~() /0
1< -- O!c,j LfC) < 10 &~'{- 0 /60 10 10
,( -- .;3 I,). cPo ....20 ~ t..fo ;2.0 ID
" '--:ot07C-J ~:o Lf'tJ ?,O ,3.;10 ' JD 10
'l -/77 (04'0 ~LJo +1'1 Lfo 10 ??'a gD ;<0 It)
---~--
I, - /J/ 9 /bo :J () 4D 10 /D :-:J...o '
'It - if,57 AJo ,;10 cPo .if,) . / l> ~O
9Z'
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In ' HAl Titers C F Titers . Trypsin
ecimen Flu A- 2 Flu A-2 Flu B Flu B Inhibitory
1-=- )a te Soecimen # Ta oan Hona Kana Md. Mass. Flu A Flu B Caoacitv
?- ~j f?- fnq lfifoo.)t.j / t; 0 40 ~o ;;;'0 10 <10 .
" -- 09.')- 1';;0 ;;to / :< .?'o. ~ J.o 40
II -IS~ '-10 If) Ifo 10 If) 1(./
I' .- .3 '10 3..:<0 ?o ?o ;).o II) 10
~- 01 Cj-{" 0 <2J'-:..'J-O 0 t.,t -~ /-10 ;2.0 1:J.,yO Iv 4"0
i\ -O.~/ '-10 dO ?o In
II - 06-/ ;20 10 40 10
II .- {):2 (.:> II.,D 1(;0 ?o ;Lo
-
(. - 0 (p(Q IC'> If) '10 10
,
Ct-J -6 (I 1003.:20 lJPo .}.O I (Il. tJ 3;1.0 ,:;1. 0 /+0
I( - 3/1 '-/0 ;)c .:1.;> D ,yO 10 10
--- -.
Ii - ~/O l(ytJ dO /~o <¥D /0 ;;2..0
I '-I -I-.
" - 3~ I 04() I; 40
-
~ - {} /2 Ibn ?o Po ¥tJ ,~O /0
I( - ;2SG 1&(') /(" 0 40 '-1-0 /;2.. 0 ,]..0
I, - D.3/.,- /&0 ;2D c?o cPo J.f-O ~O
" - d. Iv.:.t) if,)" It) /60 if-o r:?D :.20
~. -;20/ 4" It) Ir;,o ,pO 10 .l.fo
(-
I( -/If! .).() 10 '-10 .20 ;;'0 10
1\ '- 0.5-.3 ~O ID '10 dD /tJ ;;;"0
It -;)9; &, t.! U 3.'2 0 3.2D '1.;2.. 3-~o
~:/ V ~o
-
'; "- 3/P7. I,;) cPo 320 / d. cf-O I ;2.
-------
.
.
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, HAl Titers C F Titers Trypsin
- ec im en .Flu A-2 Flu A-2 Flu B fluB Inhibitory
-=date Soecimen # Ta oC'm Hona Kana Md. Mass. Flu A Flu B Caoacitv
q-j-lq /00/)107 ~-() IV ' I,)" [-¥J /D .;l. D
/2rD .
Ii - 341./ ~.2 f) 3.;10 Jf) /0 ;)..0 10
II -- ~.3;') 3~lD ?o . 10 iD 1'0 :J.()
it -~DS. ~,?o R'O d-{) ;;'D :1n .:1.0
I, - 3"!v I /...10 10 /1.-0 RD If) ;21)
II - D.lln 3:20 ..3;;'0 10 I{) tfD 10
I( -1.2 q 3-~O Lio .40 ~D /0 /0
.~
I( -1(.,7' ~.;~ b ~o -7-D .10 /..to ..z. IV
II -- O&h 3:;;'0 /0 "40 lb 0 ;),,0 dZO
I( '-/?I ~() ;lD /0 /D iO L.IO
.
II -:2 ~}I.../ 1,~!PtJ ~~:2 0 ,).,0 ;)..D I ;.10 10
I( -liR 'fD 10 10 /0 ;).() /0
(/ '- f) ,1..-0/ .3.2 D /00 ,~D dlJ /0 /0
l( '- f)l if 40 10 IV 10 II /0 (j) .33 on/" I] .f2-D / t;, f) -'-..0 10 /lJ Il)
'\ ..:...-. u~ 'I Iho J?O d).D 10 ,-'J. 0 2/)
" ,-/&3 3 ;).1) 3';;0 (7) '-10 / c'? "f)
I -
1\ -I,L.,-f:;. 3;lo /0 tic ~o 10 /0
" . '-- .3L3 '-Iv It) ,y'() cf-o /0 it)
II ~/Lf'f 1';"0 cP-O ,;20 ),.0 It)
;J.f)
II -1t./9 / to 0 Ifva :2n ;;"0 ;;"0 .-2. (.?
" -3~~ .:3~o IrbO 10 10 /.fD
,;}.!)
-
.-. (e
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-..;
I{ -3.7,.P' LID 10 /00 cYtJ /0 ::iLo
,. - t).J? /60 ID cPo d)u ';'
-------
. .
.
.
HAl Titers C F Titers Trypsin
:- ~cimen Flu A-2 Flu A-2 Flu B fluB Inhibitory
late Soecimen # Taoan Hona Kana Md. Mass. Flu A fluB Caoacitv
c-
q- .?,- (., q :2._303 'IS .~20 3,Jo )0 10 /0 ./0 .
1\ - 33l, ~O / 0 rPa /0 d-D ~o
II -:J3D /Iv 0 ...J- 0 '-10 /0 If) In
II - 07/ '-10 10 :1.0 I 0 II) 10
'I - 3 10 3,20 <10 . / &, 0 0:-'0 if) 10
.
II -- :3 L./ 10 3~D '0'-10 ,;2 0 ~o ;;'0 /0
II -"3 7t: 3;2 () 3.20 ~D .Jo .:J.. D ~o
.
1\ - /c.!J-O cPo .;2 v ;to .;2D 8vD /0
II - II ..2 bLfo 3,Jo /(,lJ It;;:o l{-O 10
II - f)(,A~ go /D / ~lJ J>D /D /0
II '- 1,.3-:;- . cPo 1-10 ~3';l 0 ¥O ' .10 10
(, -09/ ifo 10 ;;Lo ,;( 0 ,20 /0
-
II "- O.!J-..3 040 0'70' ~o 10 ¥O ;20
.. . -." .- .--
II '- -j,~ I 10 '10 s:'O Ljo d-.O ()D 10
I I I
I I
,( '- .3 (;;I-I ~? {j (J t,L/O 10 It) 10 /0
I, --IS? ?O (0 40 If 0 r2v 10
~ -- D ::;.3 40 /0 Ro (ftJ 417 if 0
I( - ()L:,7 /&D
-------
. .
.
.
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In. ~Cimen HAl Titers CF Titers '.fryp sin
Flu A- 2 Flu A-2 FluB FluB Inhibitory
Jate Soecimen # Ta oan Hona Kana Md. Mass. Flu A Flu B Caoacitv
9- j-C:Jj .~ ].) IJ O,1ZC;- /61J ~ o' /0 /0 10 ./0 .
.II - tJ47 I ;)?O hL{O /0 IV 40 If)
It - 32 '-I .3 .2 () I&;() 1:1 ,P[) ItPo 20 .:J..()
II -IDe? 3;(D ?O ;lo .;2..0 rAO /0
II "- f) 'f J . '10 ~() ?~ ;1.0 If) ,.2.0
CJ-N -b q /...It.! 0 2- 7../ 3-..20 /00 3.2.0 .0::-0 #0 :20
l( - [) 90' I fvo 020 ?o /..f() 10 'hI
I, -OW- /lvo -<0 PO :PZ> I(} /0
" "-.;2L/ 7 jtJo ;;"0 ~)..D -; It) I()
~;;~ D
I( -. (}.j-q 10'10 f?{) /0 Iv I ..:). 0 <..,0
" -';'~.3 D ,pO 10 d)..o io .~{) ..:1n
I, - IP.3 ?iJ 10 ".,20 ;J.D .,2,,0 /0
l. -- O~-b IhO-I ;).D I/~O I-fD ,-'2. () .;z. ()
I ,. h
f( - 1; 17 3.2D c?o I'lo /0 . !)... 0 il}
I, - ;;'/7 /?o 10 ?o c?() 10 /0
II "- .1() I ~) '-10. ':If). .. 'j IJ JlO Ii)
It - ObS~ .,PO ' 1-(0 ZII) L./O 10
-------
..
,
I
I.
I
I
J - . HAl Titers C F Titers Trypsin
ecimen . Flu A-2 Flu A-2. fluB Flu B Inhibitory
I-va t e Specimen # Ta Dan Hona Kana Md. Mass. Flu A Flu B Caoacitv
. 9-lf- h q 4t.fO /.P~ ?o -(fD / O. /0 JD >~ [) .
II -/&9 '10 1-'0 10 10 JO /.j1J -
I( -- / h~- /00 //) Lio ;;;o c2D 40
" - O,.5";.!J - / ;? /PO I~,D LID .;20 /0 10
Il - /9'1 0 '-10 It;.{) 100 3,;;( 0 :<'0 :J.1)
It -/L/9 cPo 10 /0 10 ,;).0 .:2 f)
1 \ - ;;(O,P ~O It) /D ID 10 ~{)
I.{ - .-;, ~rP /60 10 ?o 16D ;20 ~O
II - ,:) ob /.~?O bLjO ,pO dO d..D ;2.0
II '-;1.J7 (?o j.-/O /00 cPO /..to 'If)
Ie - /tf~ II. () ID dZO ~{O
;( -~d.7 ?O /0 Lfo /(.,0 10 ;"0
It -I?~ ~o /0 '-/0 It> ,;).0 /0
I( -liS .,;)d-O 3.1.D ;20 --2D 1(00 //J
" -/;2~ -LD /0 ;1.0 ID c?D ~o
It - 0.:23 40 /0 Ifo /0 ~/O /0
II -/;11./ 'fo /0 ifo ¥-o 10 It)
" -/~9 / :< t?o c?o 3.20 I~o ,;2. 0 / t.~
--
" -.. 0 ts 1".0 160 ;)D 1../0 ~o /0
(, -/~- '-I()
-------
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HAl Titers CF Titers Tryp sin
~cimen 'Flu A-2 Flu A-2. FluB Flu B Inhibitory
..)a t e Specimen '* Taoan Hona Kona Md. Mass. Flu A Flu B Caoacitv
q-S-fo 9 SS-Od. 0 ,;l ,p,') ,;(D /0 /0 '/0 .
10
It - D.~r~ 40 LIO It) 10 ;~D IV
II - d.-/~ / (;0 cPo .2() ~() ,.'- 0 ;20
(, - ()99 :6 ~) n ~o rFo '+0 ).0 ;2.0
,. -0...,30 40 . L.../o R() ID ~O :J.f)
{( -/39 ?O ' RD rPO q.o /0 10
l( - fJ'f3 Ljo /-10 '10 /0 /0 10
It - / 9c?' c?() ).jo r?O pt) ;;'0 iD
II -/2?" Lf() '10 .11) J.fD /0 10
-
(, -/5"9 / c;o ~;;? 0 I &.> r) ,I c.> i) ;(0 0<0
I
C(-?-to 9 J 0 {).~-4 ?D ?o ;)..0 10 --20 /0
,
If - () qC 40 <: /u ' '-/0
-------
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HAl Titers C F Titers Trypsin
~ .'.." . Flu A-2 Flu A-2' Flu B Flu'B Inhibitory
-~cimen
.Jate Specimen # Ta oan Hona Kana Md. Mass. Flu A Flu B Capaci tv
0/- E-r;q .
/ 0 :< W- if C)' /0 . /0
ItJ /v /0 .
. II - ;;;..~ 0 /&0 ?O' /0 '-10 ~O 10
Ii - 0 Ft, 1-11) /0 /0 .d
-------
.
,
I
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, HAl Titers C F Titers Trypsin
- ecimen .Flu A-2 Flu A-2, Flu B Flu B Inhibitory
Jate Soecimen * Ta oan Hona Kon a Md. Mass. Flu A Flu B Caoacitv
9-10- L q .:5 "3 0 lJ tJ < 10 ~/O <. lID ;;( I) ;1.0
II
'-IPt" /bO R~ c;'.< 0 ?D ,?O Jfo
II '- Lj I;).. '-/0' ,f7J 40 It) :7-0 10
II =- c2' Sl,~ - /blJ /-10 ?o Lfo tfO il}
I( '-- (l,~., /6D /t~ 10 la ;)..0 /0
I( - ,J. tJ 10 !G:,D 10 '--Ie /0 Ie') /0
II -D~3 ~40 ICe, 0 0 1&0
-------
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i
,- ~cimen HAl Titers CF Titers Trypsin
.Flu A-2 Flu A-2. Flu B fluB Inhibitory
)ate Specimen #- Ta oan Hona Kona Md. Mass. Flu A Flu B Caoacity
9-10-1.:- q .3~ 0 [),);:1 !tpo PO LI/) d.D ~D -JfO .
" - DIJ ~ 4D .:20 ,.;2D /D 2D ;ZO
- .
Ol. -I f:'J~ ?'O ID /..(-tJ .; lfO ,;2.D
i{ -J./3 100 10 40 ;2.0 10 J..O
.
I( .- 1(--.9 /k-O 40 /~O tv '1-0 ~o 4n'
It - DJ q 1.;.0 ?O 10 10 10 In
- 'fO!? Ilvo . f?o . c:J. 0 ,-;2 ()
I( ?O 40
h - 231' /6:0 Iff) ..~ <-2 0 320' 40 i..fo
.- 3 '1-0 J.I t, ' /0 ;).D
11. /0 ~o !O
'H -/79 .3 ,;z, 0 8>0 .3;;;. 0 ?O !-to iO
'e -OOt-, /"0 /&0 ,;20 10 H-O .,lO
.
/.( '-/~o' RO /0 /00 (P LfO I (~O IbD u_-----_._---;...
I I
, .
Il -- 3 l. ~ Yo ;20 10 10 ifo 2..0
(0: -11.f..') ; ;}}? 0 l~,'40 R-o eJ,D ?o ~o
/..{ '- '-I 0 Lj ?() .20 40 /0 10 ;2()
Ie .- t.../ 0 to PO ,').. D I(,.D / (,~,D .;2 f) :2D
(, -;(C17 ?O ...2 ()
-------
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'J HAl Titers CF Titers Tryp sin
3cimen 'Flu A-2 Flu A-2' Flu B Flu B Inhibitory
..Jate Soecimen:!t Ta Dan Hona Kana Md. Mass. FlllA Flu B Caoaci tv
Cj-II'-~-' q 4.4-0 (}3S" /7 f.] 0 1.1-0 . /0 .
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I . C F Titers
HAl Titers Tryp sin
ecimen "Flu A-2 Flu A-2. Flu B Flu B Inhibitory
Jate Soecimen # Taoan Hona Kona Md. Mass. Flu A fluB Caoacitv
. 9:...((- tj 9 Lftjo tJI (). '-10 JO ; (PO ;J.() tfD ,:).. 1) d-O
It - ,3"IG /00 10 ~D LID ,.:2 0 ;)0
Il - 0.1>.?' /60 . LjD f'() <. 10 " L/O ;2.0
If -- 0 'f I / d. cPO ~;> f./ D bLjo ~d{) dO 1-/-0
Q-/7-fp9 ~ OODJ .:<. G? L/ 0 . rrO / t.() ~o d20 r:2. i) "71
I, -/(".."-3 ,pO /D IbO cPO /0 -C..IO ~ "3
II - () '-I;;' (r-D c. (0 0 YO I{,(() /IJ LfIJ ,J> -5
't -03c? /00 10 ?D ?o r21J :;;L:J
c/
II --0.29 3"020 3;;20 10 /0 .1[.,'1 10 ..)/
It - (),," f.:, ~o 40 L-/& -
II ~ tJ.~ ~ It, 0 crf) /D 10 ;1..0 /0 ..3-S
II - 0 '1/ (.,-/0 dO ,-/0 ;;0 4/-iJ JO.
.
Ie "- ;J.. ") t-, cf1J <"'/0 JO 10 ~O /J ICf
--
II - !) O:J... LI{) /0 /0 10 /0 /0 il
-
II 100 /0 cfO tD tiC Lfo {7
"- Oy(;;.
1/ -I OS ~dO /{) cf'1) cJD 10 ¥o . ..:;.- 7
103
-------
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.. HAl Titers C F Titers Trypsin
ecimen .Flu A-2 Flu A-2, Flu B Flu B Inhibitory
Jate Soecimen -# Ta oan Hona Kona Md. Mass. Flu A fluB Capaci tv
Cj-/7-/r:;9 3000c;7 ' 1:> 40 /00 LfD 10 /0 . Ii) 3'~
1\ -07b 40 o ..lo dD 'l-n ID 7/
I( - 0.37 L.fo 10 .;20 ;2.0 /D ;/-D .. ~-4-
« - ;( bl .3dO IbO ifo c:20 /0 lO
t( - D "Iv '-10 1-../' 0 3;JD '-10 10 ;20 /1
c( -;0 IfO
Ie)
It '- 00 l:'~ //.;0' c?o ?O /f) ;;'0 dO
(. '~ 0 '13 rTO ID ~ 'J.. D :;"O ...;J.D LlO
't -- I L/ 'I / ((; 0 /hi) ~D 10 /{J /0
({ - ;( 0/ 0:<0 If) 'fi) 4-0 10 ~/O
I( ~/d~- eo rf-D
-------
.,
I-
I
1- .. HAl Titers C F Titers Trypsin
ecimen .Flu A-2 Flu A-2. Flu B Flu B Inhibitory
Jate Soecirnen # Ta oan Hono Kono Md. Mass. Flu A Flu B Caoaci tv
Q-/7-t.J q .300It?"1 /&0 ;20 I 0 ;;'0 . ....
0<. V ~o
II - 0.)0 / ;)!to /tPO /(.;.O
-------
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I--
I
f HAl Tit ers C F Titers Trypsin
':!cimen .Flu A- 2 Flu A-2. Flu B Flu B Inhibitory
I ~ate Soecimen # Ta Dan Hona Kana Md. Mass. Flu A Flu B Caoacitv
J fJ-/~-!o CJ lint) . .3.:J. () L/o
333i~57 3;)0 !() '/0 .
/( -It£" / I., D ~;2() cPO 1?o It) <'10
1< -/'13 Ilno I ::1!?0 I/'D J?o
( '- .3 OD IkO I ;) 8-0 I(..,D <-~D 10 10
II --If?'t? llao L../o /6D <10 /0 I C.?
l( -; /00 Ie '+0
'11 - 1 ..j-- ~- .3..20 :6;).0 I:J. ,J'D go ;0
-------
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-. ';)cimen 'Flu A- 2 Flu A-2 Flu B Flu B Inhibitory
. ..Jate Specimen # Ta oan Honq Konq Md. Mass. Flu A Flu B Caoacitv
3330,,39 ¥O '
!t)-/-b9 '3~o I:J. ?o I"J.!?o i..fo ';)..0
I( - to:}l./ /60 lieD ~D d,()
II - /)(.; I ,-PO 'lo 3~o ,,pO
I( -/)74 /t~o Lfo 3.lD c?O /0 /0
(( c:;..... IJ ,.p.) , / .11?o I,) J? 0 t::.LJO fio
tL -Osq ;)..o '10 3..:)0 Ljo 10 ~() .
It -- DtJ..2 ~D L./o AID /...fa .:J.. 0 .;to.
«( -- [J~3 .3:!-0 &tfo cPo '-10 -:;2 {J 10
l( "- DD.~ "Po ;2D LliJ 1..(0 10 L../O
( '- OSc-., ."3.;l 0 (;tfO 0'10 i.fo .'20 It)
I
I( -D29 /(,~o /60 3.20 ,;J.D /0 /0
'- D3D 3.;> 0 j
1\ ,~(;?.:l D Jfo /0 ' 10
'1 -{)Jij / (~() i:< (Po <3,). 0 'fo
'1 '- I) (,13 /~,D 1.J..?o 3;{o J>~ .'
II '- 1)/;2 L./D '10 (t?o L./o JI{r .~o
(I ' -- 0,,1:"' 3 1.../0 ~' /4'-;() ?D / t? it:.?
<') ~ ,~u
(I '- DO y: 1..(0 ' 4'0 (p£!O L/D 10 /0
It -I) 7 t. /Po ,,-Z,;L D /tPo 4D ,-/(.7 :;).. ()
II '-6)77 ..;LO ?o /0-0 '1-0 10 <.;z [J
-
(( - :~. II /00 10D 1::2?D -set) '/0 ;;"0
II - {)22 /?o ?o 1/.2?D ~{) d..D d-O
I( -1)'-I9 ?D cFO to r.f () ?D 10 <'/0
(( - (,) /~ ?o _3'.2 0 3.:2.0 ~o /D D '10 .:)..0 ~o
1- '
1-
1-
ID1
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