xvEPA Environmental Protection Agency Environmental Monitoring Systems Laboratory P.O Box 15027 Las Vegas NV89114 EPA 600/3-82-077 August Research and Development Animal Investigation Program 1980 Annual Report: Nevada Test Site and vicinity prepared for the U.S. Department of Energy under Interagency Agreement Number DE-AI08-76DP00539 ------- Available from the National Technical Information U.S. Department of Commerce Springfield, VA 22161 Price Code: Paper copy, A04 Microfiche, A01 Service ------- EP ,D,600/3-<)2-077 DOUDP/0C639-045 ANIMAL INVESTIGATION PROGRAM 1980 ANNUAL REPORT Nevada Test Site and Vicinity D. D. Smith, K. R. Giles Nuclear Radiation Assessment Division Environmental Monitoring Systems Laboratory D. E. Bernhardt Office of Radiation Programs, Las Vegas U. S. Environmental Protection Agency Las Vegas, Nevada 89114 Facil ity prepa red for the U.S. Department of Energy under Interagency Agreement Number DE-AI08-76DP00539 ENVIRONMENTAL MONITORING SYSTEMS LABORATORY OFFICE OF RESEARCH AND DEVELOPMENT U.S. ENVIRONMENTAL PROTECTION AGENCY LAS VEGAS, NEVADA 89114 ------- DISCLAIMER This report has been reviewed in accordance with the U.S. Environmental Protection Agency's peer and administrative review policies and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. i i ------- ABSTRACT Data are presented from the radioanalyses of tissues collected from cattle, mule deer, desert bighorn sheep, rabbits, and a horse that resided on or near the Nevada Test Site during 1980. Routine and special activities of the Animal Investigation Program are also discussed. Other than the naturally occurring potassium-40, gamma-emitting radionuclides were detected infrequently. Iodine-131 was found in the thyroid of a deer 3 weeks after a nuclear test by the People's Republic of China. Strontium-gO concentrations in bones from deer, cattle, and desert bighorn sheep were similar to those of recent years. Plutonium levels in all tissues from all species showed little variation from those levels in samples collected in recent years. Radionuclide concentrations were generally higher in the tissues of animals residing in Area 15 than in similar animals collected from other Nevada Test Site areas. Surface soil samples from the Area 15 farm contained plutonium-238 and -239 in nanocurie per kilogram concentrat ions. Hypothetical annual dose estimates to man were calculated on the basis of the daily consumption of 0.5 kilogram of liver or muscle from animals that contained peak radionuclide levels. The highest postulated dose was 0.4 millirems to whole body for cesium-137 in muscle obtained from cattle. This dose is about 0.1 percent of the 500 millirems per year radiation protection guide for individuals in the general population. All other postulated doses for consumption of tissues containing other radionuclides were less than 0.1 percent of the standard. Gross and microscopic lesions found in necropsied animals were similar to those found in animals in other areas of the U.S. and would not be attributable to ionizing radiation exposure. The movements of 26 mule deer fi tted with coll ars contai ni ng a radi 0 transmitter unit were monitored weekly. By mid-December, all deer had left their summer range on the mesas of the Nevada Test Site. In general, deer captured on Pahute Mesa spend the winter in the 40-Mile Canyon and Timber Mountain area with some movement off the NTS toward Beatty, while those captured on Rainier Mesa winter in the Shoshone Mountain area. A deer captured in December 1979 was observed in July and August 1980 in the Barley Creek area of Nye County, wh i ch is approximately 160 kilometers from its capture point. NTS mule deer population estimates were made for 1978, 1979 and 1980 through the Peterson-Lincoln index based on weekly sightings. i i i ------- iv ------- Abstract. Figures Tables. Introduction. Sample Collection Analytical Procedures and Methods Results and Discussion. Quality assurance samples. Nevada Test Site cattle. Nevada Test Site mule deer Desert bighorn sheep Nevada Test Site horse Nevada Test Site rabbits Area 15 soil Nevada Test Site spring waters Necropsy and histopathological Hypothetical dose estimates. Other Activities. Deer migration study Deer census. Beef herd. Area 15 farm. Public information Speci a1 studi es. Investigations References. List of Append i x Tab 1 es CONTENTS Page i i i vi vii 1 2 7 examinations. 9 9 9 11 11 12 12 13 13 14 14 18 18 22 23 23 24 24 24 25 28 v ------- Number FIGURES 1 2 Sampling sites on the Nevada Test Site. . . . . . . . . . . . . . . Approximate collection sites of 1980 desert bighorn sheep. . .. . . 3 Annual averages of strontium-90 in bones of cattle, deer, and desert bighorn sheep, 1956-1980 . . . . . . . . . . . . . . . 4 Mule deer capture and wintering areas, 1980 . ........... vi Page 3 6 10 21 ------- Number TABLES 1 2 Sampling Information for Nevada Test Site Cattle, 1980. Sampling Information for Nevada Test Site Animals, 1980 ...... . . . . . . 3 4 Sampling Information for Desert Bighorn Sheep, 1980 . . . . . . . . Plutonium Concentrations in Soil from the Area 15 farm, 1980. . . . 5 Tritium and Gamma Analyses of Nevada Test Site Spring Waters, 1980 . . . . . . . . . . . . . . . . . ...... 6 Summary of Peak Radionuclide Concentrations in Selected Tissues from Nevada Test Site Animals, 1980 . . . . . . . . . . . . . . . 7 Postulated Dose to Man Following Ingestion of Selected Tissues for 1 year. . . . . . . . . . . . . . . . . . . . . . . . 8 9 Deer Capture Information, 1980. . . . . . . . . .......... Deer Population Estimates by the Peterson-Lincoln Index Method for 1978, 1979, and 1980 . . . . . . . . . . . ...... vii Page 2 4 5 13 13 15 17 19 22 ------- INTRODUCTION Since 1964, the u.s. Environmental Protection Agency IS (USEPA) Environmental Monitoring Systems Laboratory at Las Vegas (EMSL-LV) has conducted the Animal Investigation Program (AlP) for the Nevada Operations Office of the U.S. Department of Energy. During 1980, the major objectives of the AlP continued to be: 1. To conduct the Nevada present in bu rdens. surveillance of domestic and wild animals on and around Test Site (NTS) in order to assess the radionuclide burden their tissues and to detect pathological effects from the 2. To investigate alleged damage to domestic animals and wildlife resulting from the activities of the Nevada Operations Office of the u.s. Department of Energy. 3. To provide public information through education and veterinary advice to the offsite population. 4. To conduct special ad hoc investigations. Progress during 1980 in achieving these objectives and summaries of the data collected are presented in this report. Previously published reports (Fountain 1961; Smith and Giles 1970, 1974, and 1975; and Smith et ale 1976, 1977a, 1977b, 1978a, 1978b, 1979, 1980, and 1981) have detailed the history, evolution, and accomplishments of the AlP since its beginning in 1957. 1 ------- SAMPLE COLLECTION Nevada Test Site animals sampled during 1980 included cattle, mule deer, rabbits and a horse. Desert bighorn sheep that range the mountainous areas to the south and east of the NTS were also sampled. Animals sampled included those that died from natural causes or through accidents, those collected through the cooperation of licensed hunters, and those that were sacrificed as part of the routine sampling activities of the program. Animals collected by rifle or shotgun fire were necropsied immediately after death and any gross pathological conditions noted. Animals collected by other means were also necropsied if a prosector was available. If advanced pos tmortem chan,]es had not occurred, the adrena 1 s, eyes, heart, kidneys, liver, lungs, muscle, spleen, thyroid, gonads, and gross lesions (if any) were sampled and prepared for histopathological evaluation. Tissues collected from large animals for radioanalyses included rumen or stomach contents, liver, lungs, tracheobronchial lymph nodes, muscle, thyroids, blood, kidneys, fetus (if present), and bone (femurs or hock). Tissues collected from the rabbits for radioanalyses included bone from the entire skeleton, muscle, skin, entire 9astrointestinal tract, and composited internal organs (liver, lungs, kidneys, and spleen). Six beef cattle from the NTS herd ~"ere sampled during 1980. Sampling information is presented in Table 1. Except for a brief weaning period, when the calves were maintained at the Area 15 farm, each animal sampled spent its entire life grazing on the Area 13 range of the NTS (Figure 1). The management of thE: beef herd and soil and range surveys of the Area 18 grazing area have been published previously (Smith 1970, Leavitt 1970, and Brown and Mason 1968). T AS LE 1. SAMPL ING I NFORMA T ION FOR NEVADA TEST SITE CATTLE, 1980 Animal Age Weight Date Number Sex (years) (kg) Sampled Remarks M 302 05/14 Bu 11 in good cond it i on 2 F 3 323 05/14 Cow in fair condition 3 F 2 week s 50 05/14 Calf in good condition, Dam was Animal No.2 4 F 7.5 523 10/08 Beginning ocular squamous cell carcinoma 5 F 7.5 500 10/08 Advanced ocular squamous cell carcinoma--pregnant 6 M 0.5 175 10/08 Bull calf in good condition 2 ------- ~ 1 . Animal Collection Location . NTS Spring MD Mule Deer '... 10 9 7 18 11 I I N R D S I Buffer Zone :I- :; "< Desert Game Range Scale on Miles o 5 10 ~~ o 5 10 15 20 10/81 Scale in Kilometers Las Vegas (65 miles from Mercury) Figure 1. Sampling sites on the Nevada Test Site. 3 ------- AlP investigators collected tissue samples (Odocoileus hemionus). Four jackrabbits (Lepus at the Area 15 experimental farm. Also a horse was sampled. Collection locations are noted on information is listed in Table 2. from four NTS adult mule deer californicus) were collected ( Equus caba 11 us) from the NTS Figure 1 and sampling Through the cooperation of State and Federal wildlife officials and participating hunters, bone and kidney samples were collected from 14 mature desert bighorn sheep rams (Ovis canadensis nelsoni) during the annual hunt. TABLE 2. Animal Number Mule Deer-1 t1u 1 e Deer-2 Mule Deer-3 ~1ule Deer-4 Horse-1 R abbit-1-A15 Rabbit-2-A15 Rabbi t-3-A15 Rabbit-4-A15 SAMPLING INFORMATION FOR NEVADA TEST SITE ANIMALS, 1980 Sex Est i mated Age (years) Est i mated Weight (kg) 80 60 50 55 420 2.2 2.3 2 2.2 Date Sampled 03/27 07/28 09/10 11/06 10/08 03/05 03/05 03/05 03/05 Remarks Collected at Mine Moun- tain, had been tracked with radiotelemetry. collar applied in June of 1977 Accident victim Area 19, lactating doe Killed during capture attempt, Echo Peak region of Area 19 Collected Trap Site, Area 19 Aged domestic horse that was euthanized Jackrabbit, collected Area 15 farm Jackrabbit, collected Area 15 farm Jackrabbit, collected Area 15 farm Jackrabbit, collected Area 15 farm M 5 F 3-4 M 1-2 :1 1.5 M 25 M 1-2 M <2 F <2 M 1-2 4 ------- Sampling information for these animals is presented in Table 3 and the collection sites are shown in Figure 2. The majority of the sheep were collected from Clark County in southern Nevada, with five coming from the Desert National Wildlife Range (DNWR). Two animals were collected from the Silver Peak and lone Mountain areas which are north and west of the NTS. An i ma 1 Number 1 2 3 4 5 6 7 8 9 10 11 12&13 14 15 TABLE 3. SAMPLING INFORMATION FOR DESERT BIGHORN SHEEP, 1980 Sex Est i ma t ed Age (years) Date Co 11 ect ed Remarks Killed at DNWR, Area 76* Killed North Eldorado Range, Area 85* Killed Arrow Canyon Range, Area 79* Killed Cathedral Mountain in Black Mountains, Area 81* Ki lled Joe i'1ay Canyon, Sheep Range DNWR, Area 77* Killed Gass Peak, Area 78* Killed Mormon Mountains, Area 80* Killed North Eldorado Range, Area 85* Kill ed Area 71 * Killed White Rock Spring, DNWR, Area 77* Killed McCullough Range, Area 84* Killed East DNWR, Area 75*. Duplicate samples were collected from the same animal and identified as No. 12 and No. 13. Killed Newberry Range, Area 86* Killed Rhyolite Ridge, Silver Peak Range, Area 70* DNWR = Desert National Wildlife Range *Nevada Wildlife Department Bighorn Sheep Management Area M M 9 6 11/19 12/07 M 5 11/21 11/15 M 8 M 8 11/23 M 5 11/21 11/16 M 5 5 11/22 11/22 M M 5 6 11/10 11/10 t~ M 8 9 11/17 M M 6 11/26 11/25 t~ 10 5 ------- ----\,. / ", / / / TTR \.. Q C. SMT / , , ------, TEMPIUTE I I 1\. HIKO COYOTE ~1T \1.. I ''\) HANCOCK SMT ( ALAMO I Lone Mt o GOLDFIELD NELLIS AIR FORCE RANGE 0) o Indicates collection site of individual animal 10 81 \ SEARCHLlG~ \ \!V \ Figure 2. Approximate collection sites of 1980 desert bighorn sheep. 6 ------- ANALYTICAL PROCEDURES AND METHODS Gamma-emitting radionuclides in samples of soft tissues and rumen contents were analyzed by gamma spectrometry. A sample of blood or of tissue was collected from each animal and analyzed for tritium. Bone samples were analyzed for strontium-89 and -90 and plutonium-238 and -239. Selected soft tissues were also analyzed for plutonium content. The EMSL-LV performed all of the analyses for tritium and the gamma-emitting radionucl ides. The Environmental Sciences Department of the Reynolds Electrical and Engineering Company, Inc. (REECo), Mercury, Nevada, performed the plutonium and strontium analyses. Rumen contents and soft tissues of sufficient volume were placed in 300-milliliter aluminum containers with a la-percent Formalin@ solution as a preservative. The containers were then sealed and stored for gamma analysis. Those tissues of smaller volume, i.e., thyroid gland, tracheo bronchial lymph nodes, etc., were fi rs t macerated in a blender and then brought to a standard volume by suspending in agar. These samples were also sealed in 300- milliliter aluminum containers. Muscle, liver, lungs, kidneys, and fetal samples were counted for approximately 1,200 minutes on lithium-drifted germanium detectors calibrated at approximately 0.5 kilo-clectronvolts (keV) per channel in the 60-KeV to 2-mega-electronvolts (MeV) range. These detectors are connected to a Nuclear Data 6620 computerized gamma spectral accumulation and analysis system. Other tissue samples such as thyroid, tracheobronch i al lymph node, sk in, and gonad, as well as rumen or stomach and gastrointestinal contents, were counted for 1,000 minutes using a 4- by 4-inch NaI(Tl) crystal also connected to the Nuclear Data 6620 for spectral accumulation. This spectrometer is calibrated at 10 KeV per channel in the 60-KeV to 2-MeV range. The data reduction of the spectra was performed using a least squares program resident on a CDC-6400 computer. Tissues collected for strontium and plutonium analyses were placed in plastic bags and kept frozen until submitted to the analytical laboratory. The samples were prepared for analyses by ashing and radiochemical separation. Plutonium was analyzed by alpha spectrometry using plutonium-236 as an internal tracer. Details of the analytical procedures used by REECo are proprietary infor/nation but are similar to those described by Talvitie 1971, 1972; Wish and f{mvell 1956; Mitchell 1960; Hagan and Arrhenius 1963; and Major et ale 1975. The EMSL-LV radionuclide analytical procedures were those described by Johns et a1. (1979). All data are reported with the 95 percent confidence interval based on counting statistics and are corrected to time of sample collection. The minimum detectable activity (MDA)is defined as 3.3 times the standard 7 ------- deviation of the background counts. Results which show a net sample activity less than the MDA are reported as such. The approximate MDAs and analytical procedures are summari zed in Append i x A. The activity values for plutonium-239 listed in this report are actually the sums of the individual isotopic activities of plutonium-239 and -240. The alpha emissions of these two isotopes cannot be separately identified (resolved) by alpha spectrometric analysis. Quality assurance samples were included in each group of samples submitted for plutonium analyses. These were either liver, muscle or bone samples purchased at a local meat market and to which a known amount of plutonium was added, or they were duplicate samples collected from sacrificed animals and submitted for analyses under blind identification numbers. The purchased bone samples were ashed prior to spiking. The data from the quality assurance samples are presented in Appendix B. Tissue and lesion samples collected for histopathological examination were fixed with a lO-percent Formalin solution and delivered to a pathologist for interpretation. 8 ------- RESULTS AND DISCUSSION QUALITY ASSURANCE SAMPLES As shown in Appendix B. the strontium-90 and plutonium-239 analytical data from a duplicate bighorn sheep bone sample indicate a precision of analyses of 4 percent and 27 percent. respectively. The accuracy of analysis, expressed as bias, is also shown in Appendix B. Based on the precision and bias data for strontium-90 analyses, the 95 percent confidence interval for the true value was calculated to range from 1.68 to 1.84 times the reported value in bone and from 1.07 to 1.23 times the reported value in soft tissue. The 95 percent confidence interval of the true value for plutonium-239 in bone ranged from 0.98 to 2.05 times the reported value while that for soft tissue ranged from 0.95 to 2.03 times the reported value. These confidence intervals were considered when conclusions were drawn from the data. NEVADA TEST SIT~ CATTLE The analytical results from tissues collected frorn the Area 18 beef catt Ie are presented in the tab 1 es of Appendi x C. Naturally occurring potassium-40 was the only gamma-emitting radionuclide consistently detected in both the May and October samples. Cesium-137 was occasionally detected in the ingesta or soft tissue samples. If detected. the c~sium-137 levels were within a narrow range of activity; for example. five of six muscle samples contained detectable levels ranging from 15 to 37 picocuries per kilogram (pCi/kg) wet weight. Tritium was not detected in the blood of any of the cattle sampled during 1980. Strontium-90 concentrations in the femur samples from the Area 18 cattle ranged from 2.4 to 3.1 picocuries per gram (pCi/g) of ash with an average of 2.8. As shown in Figure 3, this average value is similar to those reported in recent years. except for that of 1979 which was significantly lower. As explained previously (Smith et al. 1981a) the 1979 average was thought to be due to bias in the analyses by the analytical laboratory. Strontium-89 was not detected in any of the 1980 bone samples. It was planned to 1 i mit plutonium analyses to those tissues collected during the spring. because of bUdgetary restraints and the consistent findings of previous years. However, a power outage in June at the Area 15 farm resulted in the loss of all frozen samples. Because of this, the fall samples were submitted for plutonium and strontium analyses. Ingesta samples were not collected as the cattle were maintained on purchased alfalfa hay for several days prior to death. 9 ------- 30 - Bighorn Sheep ~ 25 c::::=:J 0 e e r II> c:t anm Canle .. c 0 (Number 01 Bone Samples) '" 20 ~ en '- U @ ... .E 15 II> "ii ....... '" .. 10 0 ..... en !O 5 o 1956 1951 1958 1959 1960 1961 1962 1963 1964 1965 1966 1961 1968 1969 1910 1911 1912 1913 1914 1915 1916 1911 1918 1919 1980 Figure 3. Annual averages of strontium-90 in bones of cattle, deer, and desert bighorn sheep, 1956-1980. ------- Detectable plutonium-238 concentrations were not found in any of the soft tissue samples and in only one bone sample. Liver and lung samples generally contained detectable plutonium-239 concentrations while muscle, fetal and gonad tissues did not. The lung and liver concentrations and those in the femurs (which ranged from 1.5 to 4.2 pCi/kg wet weight). were not significantly different from concentrations reported in recent years. NEVADA TEST SIrE MULE DEER The analytical results from tissues of the NTS mule deer are presented in the tables of Appendix D. As was the case for the NTS cattle, naturally occurring potassium-40 was the only gamma-emitting radionuclide that was consistently detected. Cesium-137 was detected in one kidney sample. The thyroid from deer No.4, collected on November 6,1980, contained 22:t 1.9 pCi of iodine-131 per gram (wet weight). The source of this iodine is thought to be airborne fallout from a nuclear test conducted by the People's Republic of China on October 15, 1980 (Smith, et al. 1981b). Tritium concentrations in the blood or kidneys of the deer ranged from <330 to 470 pCi/l. These levels are well within the range found in atmospheric moisture at Beatty, Nevada (Smith, et al. 1981). Strontium-90 concentrations in hock bones ranged from 0.95 to 2.2 pCi/g of ash witf-J an average of 1. 7 pCi/g ash (Figure 3). Strontium-89 was not detected in any of the bone samples. Plutonium-238 and -239 were found in measurable amounts only in the rumen content samples, one bone sample, and one gonad sample. The plutonium-239 concentration of 1.2 :t 0.52 pCi/kg in the gonads from mule deer No.3 is very similar to a value reported in 1979 (Smith, et al. 1981a). The plutonium-239 content of all lung and liver samples was below the MDA, contrary to the results of previous years (Smith, et al. 1980, 1981a). This may have been caused by the use of a different analytical laboratory for this year's samples. DESERT BIGHORN SHEEP Analytical data from bone and kidney samples are listed in Appendix E. The average strontium-90 concentration in the hock bones was 1.5 pCi/g of ash (Figure 3). Detectable levels of strontium-89, plutonium-238 and -239 were not present in any of the animals sampled. Detectable levels of plutonium-239 have generally been reported in 10 to 25 percent of the bone samples analyzed during the last few years. Cesium-137 concentrations ranging from 31 to 91 pCi/kg wet weight were detected in the kidneys of only two of the sheep. Tritium was not found in any of the kidney samples. 11 ------- NEVADA TEST SITE HORSE An aJed domestic horse from the Area 15 farm was euthanized .and sampled in October. The analytical data from the samples are listed in Appendix F. Naturally occurring potassium-40 was the only gamma-emitting radionuclide detected. A blood sample contained less than 400 pCi of tritium per liter. The bone ash contained 3.5 t 0.5 picocuries of strontium-90 per jram of ash. Net strontium-89 act ivities wel~e not reported. Net plutonium-239 activities were found in the liver and lung samples with 13 t 1.4 and 4.7 t 0.56 picocuries per kilogram of wet weight, respectively. NEVADA TEST SITE RABBITS Four jackrabbits from the Area 15 experimental farm were collected and sampled in March. Analytical data from analyses of their tissues are summarized in the tables of Appendix G. Cesium-137 and potassium-40 were present in all soft-tissue samples. Cesium-137 concentrations in muscle tissues ranged from 82 to 130 pCi/kg wet weight with a median value of 93 pCi/kg, while the values in skin ranged from 130 to 250 pCi/kg with a median value of 170 pCi/kg. The Jastrointestinal sample from rabbit No.3 contained 130 pCi of cobalt-60 and rhodium-102m, 2,300 pCi of cesium-137, and 4,300 pCi of plutonium-239 per kilogram of fresh tissue. However, the concentrations of gamma-emitting radionuclides and plutonium found in the soft tissues and bones of this animal were not significantly higher than those reported from the other three rabbits sampled. Tritium concentrations in the aqueous portion of the Area 15 rabbit tissues were all above the lower limit of detection with a median value of 4,700 pCi/l and a range of 1,200 to 6,500 pCi/l. The tritium source is believed to be the Sedan Crater which is approximately 1 mile south of the farm. Strontium-gO values in bone ranged from 1.5 to 3.5 pCi/g of ash with a median value of 1.5 pCi/g. Strontium-89 was not detected in the bone samples. Plutonium-239 was detected in 2 of 4 muscle samples and all three of the internal organ samples. These activities reported were generally higher than those found in other NTS animals sampled during 1980. Plutonium concentrations in those samples subject to external c?ntamination, i..e., the g~strointestinal.tract and the skin, were markedly hlgher than the lnternal tlssues. Plutonlum concentrations detected in the rabbit muscle tissue were usually higher than those reported for the cattle. These findings and similar ones noted for Area 15 animals in previous reports (Smith et ale 1977b, 1978a, 1978b, 1979, 1980 and 1980a) indicated that the environs of Area 15 contain elevated levels of tritium and plutonium when compared to other NTS areas that are frequently sampled by the AlP. 12 ------- AREA 15 SOIL In view of the above findings, surface soil samples (0 to 5 em) from the Area 15 farm were collected and analyzed for p1utoniurn content by the Environmental Sciences Department of REECo. As shown in Table 4, all soil samples contained plutonium with hiyher levels present in samples froln the undisturbed soil. These findings indicate that the apparently elevated plutonium concentrations reported for the gastrointestinal tract and skin samples from the Area 15 rabbits are reasonable. TABLE 4. PLUTONIUM CONCENTRATIONS IN SOIL FROM THE AREA 15 FARM, 1980 Plowed r10wed Plowed Unplowed Unplowed Unplowed Unplowed 4.8 :!.: 0.67 2.7 :!.: 0.4 2.9 :!.: 0.38 5.3 :!.: 0.64 7.5:!.: 1.4 5.8:!.: 1.1 5.3:!.: 1.0 P1utonium-238 (nCi/kg) 0.57 :!.: 0.098 0.42 :!.: 0.075 0.34 :!.: 0.054 1.3 :!.: 0.19 0.71:!.: 0.16 0.72:!.: 0.1 1.1 :!.: 0.16 Soil Type P1utonium-239 (nCi/kg) -- NEVADA TEST SITE SPRING WATERS Se~eral natural springs that serve as a source of water for wildlife were sampled during 1980. The spring locations are shown in Figure 1. The waters were analyzed for gamma-emitting radionuclides and tritium. The results are shown in Table 5. Detectable levels of gamma-emitting radionuclides were not found in any of the waters and the tritium levels were below detectable concent rat ions. TABLE 5. TRITIUM AND GAMMA ANALYSES OF NEVADA TEST SITE SPRING WATERS, 1980 Water Analysis Date Trit i um Spring Name Sampled Gamma Analysis pCi/1 Cane 5/7 GSN* <310 Topopah 5/7 GSN <310 Tippipah 5/7 GSN <310 Green 5/7 GSN <310 ~Jhite Rock 5/7 GSN <310 Oak 5/7 GSN <310 TUb 5/7 GSN <310 *GSN = Gamma Spectrum Negligible 13 ------- NECROPSY AND HISTOPATHOLOGICAL EXAMINATIONS Sacrificed animals were necropsied and selected tissue and lesion samples were collected for histopathological evaluation. The gross and microscopic pathologies observed are summarized in Appendix H. Sarcocystis spp. were detected in the skeletal and cardiac muscles of both cattle and deer. This is a ubiquitous protozoan parasite found in warm- and cold-blooded vertebrates and is usually considered to be of low pathogenicity and seldom has any clinical significance (Merck and Company 1973) . The ocular squamous cell carcinomas observed in two cows (No.4 and 5) were not unusual. Ocular carcinoma is the most common neoplasm of cattle and is especially prevalent in aged Hereford cattle subjected to high levels of sunlight (Merck and Company 1973). The most unusual lesions noted were the numerous calcified parasitic nodules found in the liver and lungs of the aged horse. Other occasionally observed histologic findings; e.g., hemosiderosis of the spleen, mild liver and kidney changes, and myositis, were felt to be within normal levels by examining pathologist. No pathology, gross or microscopic, was observed that could be attributed to ionizing radiation. HYPOTHETICAL DOSE ESTIMATES Although meat from animals living on the Nevada Test Site is not available for consumption by people, the various doses to a standard man based on postulated consumption of the edible tissues has been calculated. The dose estimates are not presented as an implication of potential doses, but rather to place the reported radionuclide concentrations in perspective. The dose estimates are based on the techniques and parameters of the International Comnission for Radiological Protection (1959, 1968, 1977) and Dillman (1969). The estimates were calculated using the maximum observed concentrations of the radionuclides in edible tissues of the cattle, deer, and rabbits sampled (summarized in Table 6). It was assumed that 500 grams (about 1 pound) of the meat were consumed each day for a year, and that the radionuclide concentra- tion remained constant for that time. The minimum detectable values of Table 6 were used in the dose calculations when they were the maximum value for a given tissue. Although doses are calculated for ingestion of tissues from a rabbit these doses are not compared to the dose guide. Furthermore these doses' are not used in discussing the maximum hypothetical doses becaus~ it is doubtful that enough similar tissue could be obtained to provide the I-year intake qua nt it i es. The International Commission for Radi?logical Protection (1959) and the U.S. Department of Energy (1977) present dlfferent dose criteria for various parts of the body, based on estimates of relative radiosensitivity. The 14 ------- TABLE 6. SUMMARY OF PEAK RADIONUCLIDE CONCENTRATIONS IN SELECTED TISSUES FROM NEVADA TEST SITE ANIMALS, 1980 Radionuclide Concentrations (pCi/kg Wet Weight) Nucl i de Muscle Liver Area 15 NTS Mule Oeer Rabbits Blood Blood (pCi/l) Muscle Liver (pCi /1 ) Muscle NA 0.051 t 0.093** 0.066 t 0.31** NA 0.00 t 0.14 NA 0.053 t 0.081** 0.13 t 0.25** NA 0.88 t 0.21 NA NO NO NA 130 t 20 <390 NA NA 470 t 250 6,500 t 340* Area 18 Cattle I-' U1 Plutonium-238 0.048 t 0.073** 0.039:!: 0.06** Plutonium-239 0.12 :!: 0.35** 0.64:!: 0.12 Cesium-137 37 :!: 12 14 :!: 8.8 Tritium NA NA NA = Not analyzed NO = Not detected * = pCi/l - aqueous portion of kidney or muscle ** = Counting error exceeds reported activity ------- National Council on Radiation Protection and Measurements (NCRP 1971) recognizes this philosophy, but recommends simplifying the guides for the general population, and uses the minimum guide (0.5 rem per year to the whole body for an individual in an uncontrolled area) for all body organs. The NCRP emphasizes that this is a simplifying administrative decision, rather than a reduction of the guides based on new technical information. The indicated confidence intervals in Table 6 are twice the standard deviation (2s) based on counting statistics. The total analytical uncertainties at two standard deviations for the plutonium results are estimated to be about 0.2 pCijkg for results of several tenths of a pCijkg, or several times the indicated counting uncertainties. Thus, differences between values, when the values are under several tenths of a pCijkg, have limited statistical significance given the total analytical uncertainties of the results. Table 7 lists postulated doses based on the data summarized in Table 6. The column on the right shows the respective fraction of the radiation protection guide of 500 millirems per year for the various postulated doses. The doses from plutonium and, to a lesser extent, cesium-137 are not delivered in 1 year. For practical purposes, however, the integrated doses for a 1-year ingestion period are related to the yearly guide. The highest postulated dose (other than for rabbit), 0.4 millirems (mrem), would result from ingesting cattle muscle. This dose is 0.1 percent of the guide of 500 mrem per year. All of the other postulated doses are about 0.2 mrem or less and are similar to or lower than those estimated for 1979 (Smith et ale 1981a). 16 ------- TABLE 7. POSTULATED DOSE TO MAN FOLLOWING INGESTION OF SELECTED TISSUES FOR 1 YEAR pCi/kg (wet weight) Dose Factor Human Organ for Animal Tissue or pCi/l mrem:pCi/da Which Dose was Containing Maxi- of (I-year Dose Percent of Nuclide Calculated mum Concentratio~ Ti ssue Water ingestion)* (mrem) 500 mrem Trit i umt Body water NTS mule deer, blood 470 :!: 250 3.6 x 10-5 0.01 <0.1 Trit i umt Body water Area 15 rabbits, muscle 6,500:!: 340 3.6 x 10-5 0.12 + Cesium-137 Whole body tissue Area 15 rabbit, muscle 130 :!: 20 0.022 1.4 =+= Cesium-137 Whole body tissue Area 18 cattle, muscle 37 :!: 12 0.022 0.49 0.1 Plutonium-238 Bone NTS mule deer, liver 0.066 :!: 0.31 0.54 <0.1 <0.1 - -....J Plutonium-238 Bone Area 18 cattle, liver 0.64:!: 0.12 0.63 0.2 <0.1 * The doses from plutonium-238 and -239, and to a lesser extent cesium-137, are not delivered within the I-year ingestion period, but, for simplification, the doses have been related to the guide for 1 year. The doses for plutonium are actually for a 50-year period, but it should be recognized that the doses are resulting from ingestion over a I-year period. The dose factors are based on a quality factor (QF) of 1 for tritium and cesium-137 and 20 for plutonium-238 and -239 (ICRP, 1977). The QF of 20 used for plutonium is twice the value used in reports for the program prior to 1979 (Smith et ale 1978b, 1979, 1980). tThe reported tritium concentrations are for muscle and kidney tissue. It is assumed that the concentration in edible tissues (water plus organic) is equal to that of the reported results. =+=The percent of the 500 mrem dose guide is not calculated for these tissues because it is not reasonable to assume that someone could consume 500 g of meat per day from NTS rabbits. sSince the analytical counting error term is much larger than the mean, the postulated dose is calculated for the mean plus the error term and is stated as a "less than" value. ------- OTHER ACTIVITIES DEER MIGRATION STUDY A sizeable mule deer herd resides in the mountainous regions of the NTS duri ng the summer. If they move to unrestricted lands, these deer may be hunted by members of the publ ic. A study to determine migration patterns of the herd through tracking of individual deer fitted with collars containing miniature radio transmitters was begun in 1975 and continued through 1980. From August through November 1980, 31 NTS mule deer (Table 8) were captured, either by chemical restraint of free-ranging animals (Smith et al. 1978a) or by trapping at a water site (Giles 1979). Ten deer were fitted with collars containing new radio transmitters, ear tags, and reflective numbers. Two deer were fitted with re-conditioned radio transmitter collars that had been recovered from deer captured prior to 1980. These 12 newly installed transmitt~rs brought to 26 the total number of working transmitters in the field (14 from previous years). AlP personnel monitored the movements of the deer weekly with hand-held receivers and directional antenna. Nineteen other deer were captured but were unsuitable for collaring and were released after visible markers (ear tags and reflective collars) had been attached. Twenty-four of the deer were captured at the Area 19 trapsite at Echo Peak and immobilized with M-99@ (etorphine). The other seven deer were free ranging animals (three on Rainier Mesa in Area 12 and four along the Pahute \1esa Road in Area 20) and were immobilized with Sernylan@(phencyclidine hydrochl ori de), and acepromazi ne ma 1 eate (Ayerest Laboratori es, Inc.) injected via a syringe-dart fired from a Cap ChurTM gun (Palmer Chemical Company). Deer captured in Areas 19 and 20 remained there until the end of December when winter storms forced them onto their wintering areas. By mid-January of 1981, nearly all the radio collared deer had moved south to the Timber Mountain in Areas 29 and 30 or to the 40-Mile Canyon area (Figure 4). From there they scattered to the Yucca Mountain and Beatty Wash areas. However there were two exceptions to this migration pattern. A buck moved west fr~m the NTS onto the Nellis Bombing and Gunnery Range in the vicinity of Black Mountain north of Beatty, Nevada and a doe moved to the northern edge of Area 19 and stayed there through the entire winter. By mid-April 1981 the deer had all moved back onto the 1980 summer ranges in Areas 19 and 20. By mid-December of 1980, two of the three radio-equipped deer captured on Rainier Mesa moved to Shoshone Mountain in Areas 16, 29 and 14. The remaining marked deer, a young buck, along with several unmarked deer of both sexes remained in Area 12 in the vicinity of N tunnel and Captain Jack Spring all winter long. All of the deer captured on Rainier Mesa returned there by early June 1981. 18 ------- TABLE 8. DEER CAPTURE INFORMATION, 1980 Age Weight Est i mate Estimate Date I. D. (years) Sex (kg) Captured Location Corrments 1 Green 2-3 F 45 7/22 Area 19, Trapsite Old No. F-1; Green Transmitter Collar (GTC) 2 Green F 28 8/5 Area 19, Trapsite Young doe lactating, GTC 3 Green 3-4 F 38 8/6 Area 19, Trapsite Lactating doe in fair condition, GTC; Dead Cat Canyon, Area 30, Nov. 27. Radio not recovered. 4 Green 4-5 F 46 8/6 1 mile west of U19C Lactating doe with 1 fawn; reservoir given ear tags GTC 5 Green 2-3 F 40 8/7 Area 19, Traps He Lactating doe in fair condition, GTC 6 Green 1-2 F 40 8/27 Area 19, TrapsHe Non-lactating doe in good condition, GTC 7 Green 1-2 M 51 8128 Rainier Mesa 1/4 Young buck in good condition, mile past Holmes 2 x 2 antlers; spent winter in Rd. turnoff Area 12, N. Tunnel and Capt. Jack Spring, GTC 8 Green 1-2 M 50 9/12 Echo Peak repeater Young buck in good condition, in Area 19 2 x 2 antlers, GTC 9 Green 4-5 M 90 9/16 Area 19, 1/4 mile Large buck, 3 x 3 antlers, east of U19F excellent condition, GTC turnoff on Pahute Mesa Rd. 10 Green 5-6 M 90 9/18 Area 19, Trapsite Large buck, 4 x 4 antlers, excellent condition, GTC 3 Blue 1-2 F 55 8/29 Rainier Mesa by Non-lactating doe in good U19R bunker condition, reconditioned blue transmitter collar 1 Red 1-2 M 68 10/2 Area 19, Trapsite Buck in good condition, 2 x 2 antlers, reconditioned red transmitter collar T 1-2 M 74 8/30 Area 19, Trapsite Buck in good condition 2 x 2 antlers, ear tags, reflective No. only 0-1 3-4 M 74 8/30 Area 19, Trapsite Buck in good condition 4 x 4 ant 1 ers, ear tag sand reflective No. only E-1 5-6 M 76 9/12 Rainier Mesa, Buck in excellent condition, Holmes Rd. turnoff 4 x 4 antlers, ear tags and reflective No. only L-1 fawn M 1.8 9/16 Area 19, Trapsite Fawn in good condition, antler nubs on head, ear tags only (continued) 19 ------- TABLE 8. (Cant; nued) Age Weight Estimate Estimate Date I. D. (yea rs) Sex (kg) Captured Location Corrments J-1 3-4 M 70 9/30 Area 19, Trapsite Buck in good condition, 4 x 4 antlers, given ear tags and reflective No. Only H-1 2-3 M 70 10/1 Area 19, Trapsite Buck in good condition, 3 x 3 antlers, given ear tags and reflective No. only K-1 fawn M 2 10/1 Area 19, Trapsite Fawn in good condition, 1-inch antler nubs on head, ear tags only M-1 2-3 M 78 10/2 Area 19, Trapsite Buck in good condition, 3 x 2 antlers, given ear tags and reflective No. only G-1 1-2 M 67 10/16 Area 19, Trapsite Buck in good condition, 2 x 2 antlers, given ear tags and reflective No. only N-1 6-7 M 98 10/9 Area 19, Trapsite Buck in excellent condition, 4 x 4 antlers, ear tags only -- too large for reflective No. P-1 6-7 M 83 10/16 Area 19, Trapsite Buck in excellent condition, 6 x 5 antlers, ear tags and reflective No. only A 4-5 M 90 11/6 Area 19, Trapsite Buck in excellent condition, 4 x 4 antlers, ear tags and reflective No. only Z 1-2 M 41 11/7 Area 19, Trapsite Buck in good condition, 2 x 2 antlers, ear tags and reflective No. only X fawn M 18 11/6 Area 19, Trapsite Fawn given ear tags only K-G 6-7 M 92 11/5 Area 19, Traps ite Buck in excellent condition, 5 x 4 antlers, ear tags and reflective No. only S-l 5-6 M 90 11/19 Area 19, Trapsite Buck in excellent condition, 7 x 5 antlers, ear tags and reflective No. only T-1 2-3 M 54 11/18 Area 19, Trapsite Buck in excellent condition, 3 x 3 antlers, given ear tags and reflective No. only F-1 3-4 M 82 11/19 Area 19, Trapsite Buck in good condition, 3 x 2 antlers, given ear tags and reflective No. only C-G 2-3 F 68 12/4 Area 19, Trapsite Doe in good condition, given ear tags and reflective No. only 20 ------- --- Summer Capture Areas Wintering Areas 9 7 3 11 ~ 1 I N.R.D.S. I Buffer Zone I I I Desert Game Range Scale In Miles ~~ o 5 10 15 20 10/81 Scale in Kilometers Las Vegas (65 miles from Mercury) Figure 4. Mule deer capture and wintering areas, 1980. 21 ------- The 1980 summer and winter ranges of the NTS deer are shown in F~gure 4. A doe tagged in December 1979 at the Echo Peak trapsite was observed ,n the Barley Creek area of Nye County during June and August 1980. This deer migrated over 160 km within 6 or 7 months. DEER CENSUS As a result of the deer migration study, there now exists on the NTS a sizeable number (approximately 125 animals marked since 1975) of identifiable deer. This base population provided the means for a statistical estimate of the deer population in certain areas of the NTS. Weekly sightings of both marked and unmarked deer during 1978, 1979, and 1980 were recorded and provided to Dr. Robert Kinnison, Battelle Pacific Northwest Laboratories, Richland, Washington, for statistical analysis. The method used was the annual weighted average of the weekly Peterson-Lincoln index estimates using capture-recapture sampling plans (Mosby 1963). The inverse of the weekly variance estimates were used as weights for averaging. These annual estimates are listed in Table 9. TABLE 9. DEER POPULATION ESTIMATES BY THE PETERSON-LINCOLN INDEX METHOD FOR 1978, 1979, AND 1980 Echo Peak Dead Horse Rainier Mesa Section Flats Section Section Area 19 .Area 19 Area 20 Area 12 Year Bucks Does Bucks Does Bucks Does Bucks Does Mean 49 15 11 8 7 8 15 21 C.1. 1978 41-56 10-20 5-17 5-11 5-9 1-15 11-20 15-28 S. D. 14 8 6 2 2 4 7 10 S. S. 13 11 5 3 6 1 9 11 Mean 47 25 6 5 28 5 8 9 C.1. 1979 32-62 17 -33 4-8 2-7 16-39 2-8 6-11 5-13 S. D. 29 15 4 3 15 4 4 5 s. s. 14 14 9 6 7 6 9 6 Mean 97 32 8 14 6 11 15 27 C.1. 1980 63-131 25-39 3-13 9-19 2-10 6-15 8-22 18-37 S. D. 48 10 6 6 6 5 9 12 S. S. 8 9 7 6 7 6 6 6 Mean - Yearly mean. C. 1. - 95% confidence interval of the yearly means. S. D. - Population standard deviation. S. S. - Sample size--number of weeks observation data available. 22 ------- The population estimates were based on weekly sightings during the period of August through November and were restricted to areas readily accessible to vehicular traffic and where marked deer were present. However, it seems logical that these population estimates could be extrapolated to other NTS areas with similar water sources and habitat. In general, the population sizes appear to be either stable or slightly increasing. Population concentrations are dependent upon the avai1ab1ity of water. As there are no known natural springs in Areas 19 and 20, the deer are dependent on precipitation or man-made water supplies such as water reservoirs, pipeline leaks, etc. The availability of these sources fluctuate with the levels of construction and testing activities carried on within these areas. BEEF HERD All animals of the beef herd were maintained on the Area 18 range for the entire year except for the 1980 calves which were weaned at the Area 15 farm during November and December. While on the range all animals subsisted on the native vegetation supplemented with cottonseed oil meal during the winter months. The herd was rounded up in May and October. At each roundup, all captured animals were identified, weighed and sprayed for ectoparasites. Health problems were treated. Selected animals were removed from the herd for sacrifice, weaning, or transfer. Following the October roundup, 35 cattle were declared surplus to the needs of the AlP and transferred to Iowa State University (ISU) Ames, Iowa. In return, ISU supplied a registered Hereford bull for use as a herd sire. A quarter horse was purchased in October for use in the semiannual roundups. AREA 15 FARM The Area 15 farm, in addition to its major function as a research facility, also served as the field station for the NTS operations of the AlP. As such it provided the facilities for the maintenance of the horses, rumen-fistu1ated steers, and beef bulls; weaning of calves; and production of alfalfa hay. Lloyd Enterprises, a contractor, operated the farm, maintained the beef herd and supplied support for studies conducted at the farm. The 16 acres of croplands produced 65 tons of alfalfa hay during 1980. This production was achieved despite severe depredation of the alfalfa by jackrabbits. The rabbits were controlled by intensive hunting. The hay production was also reduced as the alfalfa was not irrigated for a 2-week period in May when the deep-well pump had to be replaced after being damaged by a lightning strike. 23 ------- The abattoir area of the barn was divided into separate high level ("hot") and low level ("cold") radioisotope work rooms. The "hot" area (16 feet x 18 feet) is adjacent to the metabolism room and was designed to maintain a negative pressure with absolute filtration on the heating and cooling system. All ALP sampling was conducted in the "cold" area (20 feet x 20 feet x 22 feet). Both areas have their own overhead hoists and rail systems.- Other farm improvements during 1980 included repainting of the barn's interior~ landscaping of the parking area~ removal and relocation of out- buildings~ modification of the main barn's heating and water systems~ and repair and relocation of corral pens. Five surplus dairy heifers were transferred to Iowa State University in October. Eleven African Pygmy goats were transferred to the farm from the laboratory at EMSL-LV. The Area 15 farm served as a training site for participants in the Radiological Emergency Response Course sponsored by the Nuclear Regu1atory Commission~ Office of State Programs. This course, taught by REECo for DOE, NVO~ was presented on an approximate biweekly basis. PUBLIC INFORMATION The offsite public information program continued by direct contact with ranchers, and by briefings of groups touring the EMSL-LV or the NTS Experimental Farm. During 1980~ the objectives and findings of the ALP were presented to 970 NTS visitors in 42 different tour groups. Approximately 200 additional drop-in visitors to the farm received informal briefings. "Big Sam"~ the rumen-fistulated steer~ was a featured exhibit at the December 6 grand opening of the Nevada Cooperative Extension Services' Holly Park Field Laboratory in Pahrump, Nevada. Approximately 200 people visited the exhibit and were briefed on the activities and findings of the ALP. SPECIAL STUDIES During October~ November and December, the Comparative Animal Research Laboratory (CARL)~ Oak Ridge~ Tennessee~ conducted an actinide metabolism study with weaner domestic swine in the metabolism portion of the barn. Fifty young pigs were administered~ via gavage~ 5 mCi of americium-241 and sacrificed over a 3-month period for tissue uptake~ cytogenic and spermatogenesis studies. The results of this study will be published at a later date by CARL. I NVESTIGAT IONS . There were no offsite lives~o~k dama~e claims that required investigation durlng.1980. ALP personnel partlclp~ted ln the investigation of alleged plant and anlmal health effects purported ln the Three Mile Island area of Pennsylvania (Gears et ale 1980) and assisted in preparing the report. 24 ------- REFERENCES Brown, K. W. and B. J. Mason. 1968. Range Survey, Area 18, Nevada Test Site. SWRHL-52r. U.S. Department of Health, Education, and Welfare, Southwestern Radioloyical Health Laboratory, Las Vegas, Nevada. 42 pp. Dillman, L. T. 1969. "Radionuclide decay schemes and nuclear parameters for use in radiation-dose estimation." Medical Internal Radiation Dose Committee. J. Nucl. Med. Supplement No.2. Fountain, E. L. 1961. Offsite Animal Investigation Project. Fourth Annual Report. U.S. Atomic Energy Commission, Nevada Operations Office, Las Vegas, Nevada. 32 pp. Gears, G. E., G. LaRouche, J. Cable, B. Jaroslow, and D. Smith. 1980. I~vestigations of Reported Plant and Animal Health Effects in the Three Mile Island Area. NUREG-0738, EPA 600/4-80-049. U.S. Environmental Protection Agency. Environmental Monitoring Systems Laboratory, Las Vegas, Nevada. 33 pp. Giles, K. R. 1979. A summer trapping method for mule deer. EMSL-LV-0539-27. U.S. Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Las Vegas, Nevada. 5 pp. Hagan, V. 1. and G. Arrhenius. Talanta 10:865. 1963. "Ion exchange in mixed solvent." International Commission on Radiological Protection. 1959. Report of Committee lIon Permissible Dose for Internal Radiation. ICRP Report No. 2. International Commission on Radiological Protection. 1968. Evaluation of Radiation Doses to Body Tissues from Internal Contamination Due to Occupational Exposure. ICRP Report No. 10. International Commission on Radiological Protection. 1977. Recommendations of the International Commission on Radiological Protection. ICRP Report No. 26. Johns, F. B., P. B. Hahn, D. J. Thome and E. W. Bretthauer. 1979. National Environmental Research Center - Las Vegas Handbook of Radiochemical Analyt ical Methods. EMSL-LV-539-17. U.S. Envi ronmental Protect ion Agency, Environmental Monitoring Systems Laboratory, Las Vegas, Nevada. 109 pp. 25 ------- Leavitt, V. D. 1970. Soil Survey of Area 18, Nevada Test Site. SWRHL-74. U.S. Department of Health, Education, and Welfare, Southwestern Radiol~ical Health Laboratory, Las Vegas, Nevada. 119 pp. Major, W. J., K. D. Lee, and R. A. Wessman. 1975. "Analysis of 239Pu and 241Am in NAEG Large-Sized Bovine Samples." NVO-153. .!.!!.: The Radioecol~y of Plutonium and Other Transuranics in Desert Environments. M. G. White and P. B. Dunaway (eds.). U.S. Atomic Energy Commission. Merck and Company, Inc. 1973. The Merck Veterinary Manual. Fourth Edition. O. H. Siegmund (ed.). Merck and Company, Inc., Rahway, New Jersey. 1,618 pp. Mitchell, R. F. 1960. concentrations." "Electrodeposition of actinide elements at tracer Anal. Chem. 32:326. Mosby, H. S., editor. 1963. Wildlife Investigational Techniques. Second edition. The Wildlife Society. Blacksbury, Virginia. 419 pp. National Council on Radiation Protection and Measurements. Radiation Protection Criteria. Report No. 39. 1971. Basic Smith, D. D. 1970. Management History of the AEC Beef Herd - 1 June 1964 - 1 June 1969. SWRHL-80r. U.S. Department of Health, Education, and Welfare, Southwestern Radiological Health Laboratory, Las Vegas, Nevada. 26 pp. Smith, D. D. and K. R. Giles. 1970. Animal Investigation Program 1969 Annual Report. SWRHL-102r. U.S. Department of Health, Education, and Welfare, Southwestern Radiological Health Laboratory, Las Vegas, Nevada. 20 pp. Smith, D. D. and K. R. Giles. 1974. Animal Investigation Program 1970 Annual Report. NERC-LV -539-16. U. S. Envi ronmental Protect ion Agency, National Environmental Research Center, Las Vegas, Nevada. 53 pp. Smith, D. D. and K. R. Giles. 1975. 1971 Animal Investigation Program Annual Report. NERC-LV-539-20. U.S. Environmental Protection Agency, National Environmental Research Center, Las Vegas, Nevada. 39 pp. Smith, D. D., K. R. Giles, and D. E. Bernhardt. 1976. Pr~ram 1972 Annual Report. NERC-LV-539-35. U.S. Protection Agency, National Environmental Research Nevada. 32 pp. Smith, D. D., K. R. Gi les, and D. E. Bernhardt. 1977a. Animal Investigation Program 1973 Annual Report: Nevada Test Site and Vicinity. EMSL-LV-0539-3. U.S. Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Las Vegas, Nevada. 31 pp. Animal Investigation Environmental Center, Las Vegas, Smith, D. D., K. R. Giles, D. E. Bernhardt, and K. W. Brown. 1977b. Animal Investigation Pr~ram 1974 Annual Report: Nevada Test Site and Vicin- ity. EMSL-LV-0539-10. U.S. Environmental Protection Agency, Environ- mental Monitoring and Support Laboratory, Las Vegas, Nevada. 37 pp. 26 ------- Smith, D. D., K. R. Gil es, D. E. Bernhardt, and K. W. Brown. 1978a. Animal Investigation Program 1975 Annual Report: Nevada Test Site and Vicinity. EMSL-LV-0539-14. U.S. Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Las Vegas, Nevada. 48 pp. Smith, D. 0., K. R. Giles, D. E. Bernhardt, and K. W. Brown. 1978b. Animal Investigation Program 1976 Annual Report: Nevada Test Site and Vici nity. EMSL-LV-0539-20. U.S. Envi ronmental Protect ion Agency, Environmental Monitoring and Support Laboratory, Las Vegas, Nevada. III pp. Smith, D. D., K. R. Giles, D. E. Bernhardt, A. B. Crockett, and R. R. Kinnison. 1979. Animal Investigation Program 1977 Annual Report: Nevada Test Site and Vici nity. EMSL-LV-0539-26. U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory. Las Vegas, Nevada. 90 pp. Smith, D. 0., D. E. Bernhardt, and K. R. Giles. 1980. Animal Investigation Program 1978 Annual Report: Nevada Test Site and Vicinity. EPA-600j3-80-096, DOEjDPj0059-038. U.S. Environmental Protection Agency. Environmental Monitoring Systems Laboratory, Las Vegas, Nevada. 65 pp. Smith, D. D., K. R. Giles, and D. E. Bernhardt. 1981a. Animal Investigation Program 1979 Annual Report: Nevada Test Site and Vicinity. EPA-600j 3-81-035, DOEjDPj00539-042. U.S. Environmental Protection Agency. Environmental Monitoring Systems Laboratory, Las Vegas, Nevada. 65 pp. Smith, D. 0., R. F.. Grossman, W. D. Corkern, D. J. Thome, R. G. Patzer, and J. L. Hopper. 1981b. Offsite Environmental Monitoring Report: Radiation Monitoring Around United States Nuclear Test Areas, Calender year 1980. EPA-600j4-81-047, DOEjDPj00539-043. U.S. Environmental Protection Agency. Environmental Monitoring Systems Laboratory, Las Vegas, Nevada. 101 pp. Talvitie, N. A. 1971. "Radiochemical determination of plutonium in environmental and biological samples by ion exchange." Anal. Chem. 43: 1827-1830. Talvitie, N. A. 1972. "Electrodeposition of actinides for alpha spectrometric determination." Anal. Chem. 44:280-282. U.S. Department of Energy. 1977. "Standards for Radiation Protection r~anual." U. S. Department of Energy Transmitta 1 Not ice. Chapter 0524. Wish, L. and M. Rowell. 1956. Sequential Analysis of Tracer Amounts of Np, U, and Pu in Fission-Product Mixtures by Anion Exchange. USNRDL-TR-117. Naval Radiological Defense Laboratory, San Francisco, California. 34 pp. 27 ------- 0-3 0-4 0-5 E-1 LIST OF APPENDIX TABLES Table A-I Summary of Analytical Procedures and Minimum Detectable Act i vi ties. . . . . . . . . . . . . . . . . . . . . . . . . . . B-1 C-1 Qual ity Assurance Results. . . . . . . ..... ........ Plutonium Concentrations in Lungs and Tracheobronchial Lymph Nodes, Beef Cattle, Area 18, NTS, October 1980. . . . . . . . . C-2 Plutonium Concentrations in Muscles and Livers, Beef Cattle, Area 18, NTS, October 1980. . . . . . . . . . . . . . . . . . . C-3 Plutonium Concentrations in Gonads and Fetus, Beef Cattle, Area 18, NTS, October 1980. . . . . . . . . . . . . . . . . . . C-4 Plutonium and Strontium Concentrations in Femurs, Beef Cattle, Area 18, NTS, 1980. . . . . . . . . . . . . . ..... C-5 Gamma-Emitting Radionuclide and Tritium Concentrations in Selected Tissues, Beef Cattle, Area 18, NTS, May 1980 . . . . . C-6 Gamma-Emitting Radionuclide and Tritium Concentrations in Selected Tissues, Beef Cattle, Area 18, NTS, October 1980 . . . 0-1 Plutonium Concentrations in Muscles and Livers, Mule Deer, NTS, 1980 . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-2 Plutonium Concentrations in Lungs and Gonads, Mule Deer, NT S, 1980 . . . . . . . . . . . . . . . . . . . . . . ..... Plutonium Concentrations in Rumen Contents, Mule Deer, NT S, 1980 . . . . . . . . . . . . . . . . . . . . . . ..... Plutonium and Strontium Concentrations in Hock Bones, Mule Deer, NTS, 1980. . . . . . . . . . . . . . . . ...... Gamma-Emitting Radionuclide and Tritium Concentrations in Selected Tissues, Mule Deer, NTS, 1980. . . . . . . . . . . . . Plutonium and Strontium Concentrations in Bones, Desert Bighorn Sheep, 1980 . . . . . . . . . . . . . . . . . . . 28 Page 30 31 33 34 35. 36 37 38 39 40 41 42 43 44 ------- Table E-2 F-1 G-1 LIST OF APPENDIX TABLES (Continued) Gamma-Emitting Radionuclide and Tritium Concentrations in Kidneys, Desert Bighorn Sheep, 1980 . . . . . . . . . . . . . . Plutonium, Strontium, and Gamma-Emitting Radionuclide Concentrations in Selected Tissues, NTS Horse, 1980 . . . . . . Plutonium Concentrations in Selected Tissues, Rabbits, Area 15, NTS, 1980. . . . . . . . . . . . . . . . . . . . . . . G-2 Plutonium and Stontium Concentrations in Bones, Rabbits, Area 15, NTS, 1980. . . . . . . . . . . . . . . . . . . G-3 H . . . . Gamma-Emitting Radionuclide and Tritium Concentrations in Selected Tissues, Rabbits, Area 15, NTS, 1980 . . . . . . . . . Gross and Microscopic Pathology Found in Necropsied Animals, 1980 . . . . . . . . . . . . . . . . . . ....... 29 Page 46 47 48 50 51 52 ------- TABLE A-I. SUMMARY OF ANALYTICAL PROCEDURES AND MINIMUM DETECTABLE ACTIVITIES Type of Analysis Analytical Equipment Count i 119 Period (min) Analytical Procedures Sample Size Minimum Detectable Activities (pCi/total sample)* For: SI;Mn, 60Co, 9SZr, 103Ru, 121;Sb, 132Te, 1311, 131;1, 137CS, II;0Ba - 7 pCi 12SSb, II;ICS, - 30 pCi 6SZn, 106Ru, Il;l;Ce - 20 pCi 181W - 85 pCi 7Be - 45 pCi Gamma Spectrom- etry 89-90Sr w o 3H 238- 239Pu Lithium-drifted germanium detectors calibrated at approximately 0.5 KeV per channel input to 4096 channels resident in the core of the Nuclear Data 6620 computer-based gamma spectrometry system. or by 10-cm-thick by 10 em-diameter Na! (Tl-activated) crystal with input to Nuclear Data 6620 calibrated at 10 keY per channel in the 60 keY to 2 MeV range. Low-background thin-window, gas-flow proportional counter with a 2.54-cm diameter window (900 ~g/cm2). Automatic liquid scintillation counter with output printer. Alpha spectrometer with silicon surface barrier detectors operated in vacuum chambers. 1,200 1,000 200 200 1,000 Radionuclide concentrations quantitated from gamma spectrum by Nuclear Data 6620 computer-based gamma spectrometry system using vendor supplied software. Radionuclide concentration quantitated by COC-6400 computer using Least Squares Technique. Chemical separation by Sr (N03)2 precipitation and Fe(HO)3 scavenge 89-90Sr was counted as SrC03. 90Sr was determined by 90y counting. Purity was checked by two successive counts at 14-day intervals for 89-90Sr and 3 separate counts at 24-hour intervals for 90y. Sample prepared by distillation. Ash sample is digested with acid, purified by ion exchal1ge, electroplated on stainless steel planchet, and counted by alpha spectrometer. 300-ml al umi num cans 300-ml a1 umi num cans 10 g of ash 5 m1 0.1-1 kg wet weight 1-10 g of ash For: For: For: For: For: For: For: 21;1Am - 35 pCi 22Na - 4 pCi K - 0.08 g For: 89Sr - .5 pC i 90Sr - .3 pCi 0.4 pCi/m1 H20 For all isotopes - 0.04 pCi * The minimum detectable activities in terms of total activity per sample for standard geometries and counting times are defined as those activities which equal 3.29 times the standard deviation based on counting only [Environmental Measurements Laboratory (EML-formerly HASL) Procedures Manual, HASL-300, pages 0-08-02 through 0-08-06, August 1974]. These values are approximations and are applicable to ideal conditions and simple complexes of nuclides. Complex spectra or spectra showing naturally occurring radionuclides can raise the minimum detectable activities considerably. ------- TABLE B-1. QUALITY ASSURANCE RESULTS Sample and Act i vity Added Activity Reported Percent Shipment No. Nuclide (pCi/g Ash) (pCi /g Ash) Bias* Bone Ash Samples 179960 90Sr 0 1. 3 :!: O. 18 No.3 239Pu 0 <0.013 179961 90Sr 1.5 1.3 :!: 0.35 100 No.3 239pu 0.022 <0.0075 100 179962 90Sr 1.5 1. 8 :!: O. 25 73 No.3 239Pu 0.022 <0.0082 100 179963 90Sr 12.0 5.4 :!: 0.73 67 No.3 2 3 9P u 0.088 0.045 :!: 0.0079 62 179964 90Sr 12.0 6.7 :!: 0.9 56 No.3 239Pu 0.088 0.037 :!: 0.0062 58 221119 90Sr 0 1. 4 :!: 0.2 No.9 2 3 9P u 0 0.0076 :!: 0.0181 221120 90Sr 3.0 1. 2 :!: O. 18 100 No.9 23 9Pu 0.044 0.041 :!: 0.009 33 221121 90Sr 2.9 1.8 :!: 0.25 86 No.9 239pu 0.044 0.043 :!: 0.009 28 221147 90Sr 0 1. 6 :!: O. 27 No.13 23 9pu 0 0.024 :!: 0.0086 221148 90Sr 7.3 5.3 :!: 0.27 47 No. 13 239pu 0.055 0.076 :!: 0.017 -17 Duplicate Bone Samples for Bighorn Sheep No. 12 Precision of Ana lys i s** 221115 90Sr 0 1.3:!: 0.18 No.9 239pU 0 0.0069 :!: 0.0078 221116 90Sr 0 1.2 :!: O. 17 0.04 No.9 2 3 9p U 0 0.012 :!: 0.012 0.27 (continued) 31 ------- TABLE B-1. (Cont i nued ) Sample and Shipment No. Act i vity Added (pCi /kg t) Activity Reported ( p C i /k g t ) Nuclide Percent * Bias Muscle Tissue Samples 179949 90Sr 56.0 46.0 :!: 8.8 No.3 239pU 8.4 4.0 :!: 0.54 179954 90Sr 130.0 120.0 :!: 16.0 No.3 239Pu 13.1 6. 7 :!: 1. 8 179956 90Sr 0 O. 51 :!: O. 11 No.3 239pu 0 <0.009 179958 90Sr 24.0 29.0 :!: 4.3 No.3 23 9pu 2.4 1. 2 :!: O. 22 Liver Tissue Samples 179950 90Sr 140.0 83.0:!: 11.0 No.3 239pu 13.0 6.4 :!: 0.9 179955 90Sr 0 <2.0 No.3 239pu 0 <0.028 179957 90Sr 25.0 23.0 :!: 3.8 No.3 239pu 2.5 1. 7 :!: 0.57 159959 90Sr 48.0 36.0 :!: 5.2 No.3 239Pu 7.2 3. 1 :!: 0.66 *8 i as ( B) = 1 - Recovery where Recovery is average recovery (:i) where xi = net activity reported u = activity added **Precision (Cv) = xl - X2 Xl + X2 where xl = first value x2 = second value The formulas for 95% confidence interval limits: L upper = xi (1 + B + 2Cv) L 1 ower = X i (1 + B-2 C v ) t Wet weight 32 19 53 8. 1 49 -19 51 41 51 12 33 17 58 ------- TABLE C-1. PLUTONIUM CONCENTRATIONS IN LUNGS AND TRACHEOBRONCHIAL LYMPH NODES, BEEF CATTLE, AREA 18, NTS, OCTOBER 1980 Animal Number Plutoniurn-238 (pCi/g Ash) (pCi /kg*) Plutonium-239 (pCi/g Ash) (pCi /kg*) Ash (% ) Lung s 4 0.0063 :t 0.012** 0.055 :t 0.11** 5 0.0007 :t 0.00032** 0.006 :t 0.028** 6 0.0062 :t 0.029** 0.029 :t 0.13** iv1 ed i a n 0.0062** 0.029 0.14:t 0.026 1.2 :t 0.23 0.88 0.17 :t 0.027 1. 5 :t 0.24 0.88 0.057 :t 0.099** 0.26 :t 0.46** 0.46 0.14 1.2 0.88 Rang e 0.0007** - 0.0063** 0.057** - 0.17 0.006** - 0.055** 0.26** - 1.5 0.46 - 0.88 Tracheobronchial Lymph Nodes 4 0.021 :t 0.097** 0.12:t 0.55** 5 -0.13:t 0.26** -2.8 :t 5.9** 6 0.01 :t 0.049** 0.12 :t 0.57** Med i an 0.01 ** 0.12** Range -0.13** - 0.021** -2.8** - 0.12** 0.56 2.8 :t 0.53 15 :t 3.0 0.15:t 0.29** 3.3:t 6.4** 2.2 0.042 :t 0.097** 0.49 :t 1. 1 ** 1. 2 0.15 3.3 1.2 0.042** - 2.8 0.49** - 15 0.56 - 2.2 * Wet weight ** Counting error exceeds reported activity. 33 ------- TABLE C-2. PLUTONIUM CONCENTRATIONS IN MUSCLES AND LIVERS, BEEF CATTLE, AREA 18, NTS, OCTOBER 1980 Plutonium-238 Plutonium-239 (pCi /g Ash) (pCi /g Ash) Ash Animal Number (pCi /kg*) (pCi fkg*) (%) Muscles 4 0.00044 I 0.002** 0.00044 I 0.002** 0.85 0.0037 I 0.017** 0.0037 I 0.017** 5 0.0061 I 0.0094** 0.016 I 0.045** 0.78 0.048 I 0.073** 0.12 I 0.35** 6 0.00055 I 0.0025** 0.0077 I 0.01** 0.81 0.0044 I 0.02** 0.062 I 0.081** Med i an 0.00055** 0.0077** 0.81 0.0044** 0.062** Rang e 0.00044** - 0.0061** 0.00044** - 0.016** 0.78 - 0.85 0.0037** - 0.048** 0.0037** - 0.12** Livers 4 0.0021 I 0.0057** 0.056:t 0.016 1 0.021 I 0.057** 0.56 I 0.16 5 0.0048 I 0.0073** 0.08 I 0.015 0.81 0.039 I 0.06** 0.64 I 0.12 6 0.0013 I 0.003** 0.013 I 0.0043 1 0.013 I 0.03** 0.13 I 0.043 Med i an 0.0021** o. 056 1 0.021** 0.56 Range 0.0013** - 0.0048** 0.013 - 0.08 0.81 - 1 0.013** - 0.039** 0.13 - 0.64 * Wet weight ** Count i ng error exceeds reported act i vi ty. 34 ------- TABLE C-3. PLUTONIUM CONCENTRATIONS IN GONADS AND FETUS~ BEEF CATTLE~ AREA 18, NTS, OCTOBER 1980 .,---------~-- Animal Number Plutonium-238 (pCi/g Ash) (pCi/kg*) Plutonium-239 (pCi/g Ash) (pCi/kg*) Ash (%) Gonads 4 0.015 :!: 0.034** 0.55 :!: 1. 3** 5 -0.069 :!: 0.12** -0.6 :!: 1. 1 ** 6 0.0027 :!: 0.0042** 0.03 :!: 0.047** Median 0.0027** 0.03** 0.029 :!: 0.048** 1. 1 :!: 1. 8 ** 3.8 0.02 :!: 0.065** 0.17 :!: 0.56** 0.87 0.0006 :!: 0.002** 0.0067 :!: 0.022** 1. 1 0.02** 0.17** 1.1 Rang e -0.069** - 0.015** 0.0006** - 0.029** -0.6** - 0.55** 0.0067** - 1.1** 0.87 - 38 5 0.0069 :!: 0.013** 0.12 :!: 0.22** Fetus 0.0012 :!: 0.0054** 0.02 :!: 0.092** 1.7 * Wet weight ** Counting error exceeds reported activity. 35 ------- TABLE C-4. PLUTONIUM AND STRONTIUM CONCENTRATIONS IN FEMURS, BEEF CATTLE, AREA 18, NTS, 1980 Plutonium-238 Plutonium-239 Strontium-89 Strontium-90 An i mal (pCi /g Ash) (pCi/g Ash) (pCi/g Ash) (pCi /g Ash) Ash Number (pCi /kg*) (pCi /kg*) (pCi/kg*) (pCi /kg*) (%) 4 0.00041 I 0.0019** 0.012 I 0.0045 12 I 18** 2.4 I 0.35 35 0.14 I 0.67** 4. 2 I 1. 6 4,200 I 6,300** 840 I 120 5 0.0072 I 0.0031 0.0059 I 0.0069** 11 :t 20** 3 :t 0.42 25 1.8 I 0.78 1. 5 I 1. 7** 2,800 :t 5,000** 750 :t 105 w ~ 6 0.0007 :t 0.0033** 0.016 I 0.00034 17 :t 26** 3.1 :t 0.49 20 0.14 I 0.66** 3.2 :t 0.68 3,400 :t 5,200** 620 :t 98 Med i an 0.0007** O. 012 12 3 25 0.14** 3.2 3,400 750 Ra~e 0.00041** - 0.0072 0.0059** - 0.016 11** - 17** 2.4 - 3.1 20 - 35 O. 14 ** - 1. 8 1. 5** - 4.2 2,800** - 4,200** 620 - 840 Frozen samples from animals 1-3 were lost because of a power outage at 16 Area 15 farms. * Wet weight ** Counting error exceeds reported activity. ------- TABLE C-5. GAMMA-EM ITTI NG RAD IONUCLIDE AND TRI TI UM CONCENTRATIONS IN SELECTED TISSUES, BEEF CATTLE, AREA 18, NTS, MAY 1980 Muscles Livers Ki dneys Rumen Contents An i mal K (g/kg*) Lung s K (g/kg*) Thyroids K (g/kg*) K (g/kg*) Blood Number 137Cs(pCi/kg*) K (g/kg*) 137Cs(pCi/kg*) K (g/kg*) 137CS(pCi/kg*) 137CS(pCi/kg*) 3H (pC i /1 ) 1 6.4:!: 0.29 16 :!: 1.5 Sample Lost 41 :!: 3.6 4.1:!: 0.33 3.3 :!: 0.33 <320 37 :!: 12 <27 36 :!: 17 2 6.1 :!: 0.32 7.6 :!: 0.64 3.8:!: 0.22 35 :!: 2.6 5. 1 :!: O. 28 2.6 :!: 0.3 <320 21 :!: 12 14 :!: 8.8 29 :!: 12 <37 w ""-J 3** 6.0:!: 0.3 7.2:!: 0.4 5.4:!: 0.31 GSN 8.2:!: 0.61 Not Sampled <320 33 :!: 12 <24 81 :!: 26 Median 6.1 7.6 4.6 39 5. 1 3.0 <320 33 <19 29 <37 Range 6.0 - 6.4 7.2 - 16 3.8 - 5.4 35 - 41 4.1 - 8.2 2.6 - 3.3 <320 - <320 21 - 37 14 - <24 <27 - 81 36 - <37 * Wet weight sampled; only K at 7.3 :!: 0.8 g/kg was detected. ** Thymus was ------- TABLE C-6. GAMMA-EMITTING RADIONUCLIDE AND TRITIUM CONCENTRATIONS IN SELECTED TISSUES, BEEF CATTLE, AREA 18, NTS, OCTOBER 1980 Muscles An i mal K (g/kg*) Lungs Livers Thyroids Kidneys Blood Number 137CS(pCi/kg*) K (g/kg*) K (g/kg*) K (g/kg*) K (g/kg*) Rumen Contents** 3H (pCi /1 ) 4 3.5 I 0.26 6.8 I 0.43 4.7 I 0.37 22 I 3.4 4.1 I 0.25 Not Sampled <390 <25 5 6.2 I 0.27 7.9 I 0.77 4.5 I 0.29 9.6 I 1. 4 4.4 I 0.26 Not Sampled <390 15 I 9.2 w OJ 6 5.8 I 0.28 4.2 I 0.33 4.8 I 0.28 4.1 I 0.56 3.4 I 0.24 Not Sampled <390 24 I 8.8 Median 5.8 6.8 4.7 9.6 4.1 <390 24 Rang e 3. 5 - 6.2 4. 2 - 7.9 4.5 - 4.8 4.1 - 22 3.4 - 4.4 <390 - <390 * Wet weight ** Rumen Contents not sampled as animal maintained on alfalfa hay for several days prior to death. ------- TABLE 0-1. PLUTONIUM CONCENTRATIONS IN MUSCLES AND LIVERS, MULE DEER, NTS, 1980 Animal Number Plutonium-238 (pCi/g Ash) (pCi /kg*) Plutonium-239 (pCi/g Ash) (pCi /kg*) Ash (% ) ~~uscles 1 -0.0012 ~ 0.0037** -0.013 ~ 0.059** 2 -0.0015 ~ 0.0071** -0.0095 ~ 0.044** 3 0.0051 ~ 0.0093** 0.051 :t 0.093** 4 0.00059 :t 0.0028** 0.0044 ~ 0.021** Median -0.0003** -0.0026** 0.013 ~ 0.004 0.13 ~ 0.042 1 0.0061 :t 0.014** 0.038 ~ 0.09** 0.63 0.0051 :t 0.0093** 0.051 .t 0.093** 1 0.0072 :t 0.011** 0.053 :t 0.081** 0.74 0.0067** 0.052** 0.87 Rang e -0.0015** - 0.0051** 0.0052** - 0.013 -0.0095** - 0.051** 0.038** - 0.13 0.63 - 1 Livers 1 0.0031 :t 0.0054** 0.039 1: 0.069** 2 0.0013 :t 0.0061** 0.021 :t 0.1** 3 0.006 ~ 0.028** 0.066:t 0.31** 4 0.0019 :t 0.009** 0.018 ~ 0.087** Median 0.0022** 0.036** Range 0.0013** - 0.006** 0.018** - 0.039** 0.012 ~ 0.0048 0.15 :]: 0.06 1.3 0.0078 :t 0.015** 0.13 :t 0.25** 1.6 0.006 :t 0.028** 0.066 :]: 0.031** 1.1 0.0039 :t 0.013** 0.038 :t 0.13** 0.97 0.0069** 0.098** 1.2 0.0039** - 0.012 0.038** - 0.15 0.97 - L 6 * Wet weight ** Counting error exceeds reported activity. 39 ------- TABLE 0-2. PLUTONIUM CONCENTRATIONS IN LUNGS AND GONADS, MULE DEER, NTS, 1980 Animal Number Plutonium-238 (pCi/g Ash) (pCi /kg*) Plutonium-239 (pCi/g Ash) (pCi /kg*) Ash (%) 1 2 3 4 i~ ed i a n Range 3 4 Lu ng s -0.002 I 0.009** 0.044 I 0.014 0.68 -0.013 I 0.062** 0.3 I 0.094 -0.0074 I 0.0016** 0.003 I 0.0097** 1.2 -0.088 I 0.18** 0.035 I 0.12** 0.0015 I 0.0071** 0.0045 I 0.012** 1.2 0.018 I 0.085** 0.054 I 0.14** 0.0011 I 0.005** 0.011 I 0.018** 1.1 0.012 I 0.055** 0.12 I 0.2** 0.0004** 0.007** 1.2 0.00 0.087** -0.0074** - 0.0015** 0.003** - 0.044 0.68-1.2 -0.088** - 0.018** 0.035** - 0.3 Gonads 0.014 I 0.033** 0.088 I 0.037 1.4 0.2 I 0.46** 1.2 I 0.52 0.0021 I 0.0096** 0.0041 I 0.014** 2.3 0.048 I 0.22** 0.095 I 0.32** * Wet weight ** Counting error exceeds reported activity. 40 ------- TABLE 0-3. PLUTONIUM CONCENTRATIONS IN RUMEN CONTENTS, MULE DEER, NTS, 1980 Plutonium-238 Plutonium-239 (pCi/g Ash) (pCi/g Ash) Ash Animal Number (pCi/kg*) (pCi /kg*) (%) 1 0.0072 :t 0.0024 0.038 :t 0.0063 1.8 0.13 :t 0.044 0.69 :t 0.11 2 0.007 :t 0.0082** 0.027 :t 0.0079 2.2 0.15 :t 0.2** 0.61 -t 0.17 3 0.006 :t 0.0081** 0.034 :t 0.0085 1.5 0.09 :t 0.12** 0.51 :t 0.13 4 0.025 :t 0.0074 0.23 :t 0.037 1.3 0.33:t 0.096 3.0 :t 0.48 Med i an 0.0071 ** O. 036 1.7 0.14** 0.65 Range 0.006** - 0.025 0.027 - 0.23 1. 3 - 2. 2 0.09** - 0.33 0.51 - 3.0 * Wet weight reported act i vity. ** Counting error exceeds 41 ------- TABLE 0-4. PLUTONIUM AND STRONTIUM CONCENTRATIONS IN HOCK BONES, MULE DEER, NTS, 1980 Plutonium-238 Plutonium-239 Strontium-89 Strontium-90 An i ma 1 (pCi /g Ash) (pCi/g Ash) (pCi/g Ash) (pCi/g Ash) Ash Number (pCi/kg*) (pCi /kg*) (pCi /kg*) (pCi /kg*) (%) 1 -0.0011 t 0.0037** 0.019 t 0.0071 450 t 510** 2.2 t 0.3 25 -0.28 t 0.93** 4.9 t 1.8 110,000 t 130,000** 550 t 76 2 Sample Lost ~ N 3 0.0004 t 0.0018** 0.0056 t 0.007** 16 t 16** 0.95 t 2.0** 22 0.088 t 0.4** 1. 2 t 1. 5 ** 3,500 t 3,500** 200 t 440** 4 0.0004 t 0.0018** 0.0004 t 0.0018** -176 t 230** 1. 9 t 0.41 33 O. 13 t 0.59** O. 13 t 0.69** 58,000 t 76,000** 630 t 140 Median 0.0004** 0.0056** 16** 1.9 25 0.088** 1.2** 3,500** 550 Range -0.0011** - 0.0004** 0.0004** - 0.019 -0.28** - 0.13** 0.13** - 4.9 -176** - 452** -5,8000** - 110,000** 0.95** - 2.2 200** - 630 22 - 33 * Wet weight ** Counting error exceeds reported activity. ------- TABLE 0-5. GAMMA-EM ITTI NG RADIONUCLIDE AND TRITIUM CONCENTRATIONS IN SELECTED TISSUES, MULE DEER, NTS, 1980 Ki dneys An i mal Muscles Lungs Livers Thyroids K (g/kg*) Rumen Contents Blood Number K (g/kg*) K (g/kg*) K (g/kg*) 1311 ( pC i /g *) 137Cs(pCi/kg*) K (g/kg*) 3H (pCi/l) 1 4.9 :t 0.33 6.9 :t 0.44 3.9:t 0.31 <4 4.3 :t 0.32 4. 1 :t 0.31 <420 <30 2 3.6 :t 0.29 4.2 :t 0.36 4.4:t 0.36 Sample Not 4.3:t 0.33 3.6:t 0.29 470 :t 250 Collected 19 15 .j:::a 5.0:t 0.32 w 3 4.5 :t 0.26 8.2 :t 0.47 Sample Not 7.4:t 0.48 5.4:t 0.31 <330 Collected <50 4 5.8 :t 0.31 5.0 :t 0.43 5.3:t 0.35 22 :t 1. 9 9.5:t 0.65 5.0 :t 0.33 <420 <73 Median 4.7 6.0 4.7 <13 5.9 4.6 <420 <40 Range 3.6 - 5.8 4.2 - 8.2 3.9 - 5.3 <4 - 22 4.3 - 9.5 3.6 - 5.4 <330 - 470 19 - <73 * Wet weight ------- TABLE E-1. PLUTONIUM AND STRONTIUM CONCENTRATIONS IN BONES, DESERT BIGHORN SHEEP, 1980 Plutonium-238 Plutonium-239 Strontium-89 Strontium-90 Ani mal (pCi/g Ash) (pCi /9 Ash) (pCi/g Ash) (pCi/g Ash) Ash Number (pCi /kg*) (pCi /kg*) (pCi /kg*) (pCi/kg*) (%) 1 0.0042:t 0.011** 0.02 :t 0.027** -1.0:t 7.9** 1. 9 :t o. 34 18 0.76 :t 2.0 3.6 :t 4.9 -180 :t 1,400 340 :t 61 2 0.00041 :t 0.0019** 0.002 :t 0.0042** 0.55 :t 2.2** 0.55 :t 0.086 34 0.14:t 0.65 0.68 :t 1. 4 190 :t 750** 190 :t 30 3 O. 0064 :t O. 011** 0.0032 :t 0.0074** 4. 2 :t 11 ** 2.2 :t 0.33 34 2.2 :t 3.7 1. 1 :t 2.5 1,400 :t 3,700 750 :t 110 4 0.0034 :t 0.0072** 0.0069:t 0.010** 1. 4 :t 3.8** 0.68 :t O. 11 31 1.1 :t 2.2 2. 1 :t 3. 1 430:t 1,200** 210 :t 34 ..j:::. ..j:::. 5 0.0044 :t 0.0078** 0.00063:t 0.0029** -1.1 :t 9.0** 1.7 :t 0.27 28 1. 2 :t 2.2 0.18 :t 0.81 -300 :t 2,500 470 :t 76 6 Sample Lost 7 0.00036 :t 0.0017** 0.0029:t 0.0048** o. 41 :t 8. 8 ** 2.1 :t 0.29 33 0.12:t 0.56 0.96:t 1.6 140 :t 2,900 690 :t 96 8 0.0004:t 0.0018** 0.0056:t 0.007** 1.7 :t 3.1** 0.58 :t O. 11 25 0.1 :t 0.45 1.4 :t 1.8 430 :t 780 150 :t 28 9 0.00042 :t 0.0019** 0.0089:t 0.0094** -1. 5 :t 5.9** 1. 5 :t O. 2 15 0.063 :t 0.29 1.3 :t 1.4 -230 :t 890 230 :t 30 10 0.00031 :t 0.0015** O. 013 :t O. 0042** 3.0:t 6.5** 1.3:t 0.19 26 0.081 :t 0.39 3.4 :t 1.1 780 :t 1,700 340 :t 49 (continued) ------- TABLE E-1. (Continued) Plutonium-238 Plutonium-239 Strontium-89 Strontium-90 Ani ma 1 (pCi/g Ash) (pCi/g Ash) (pCi /g Ash) (pCi/g Ash) Ash Number (pCi /kg*) (pCi /kg*) (pCi/kg*) (pCi /kg*) (%) 11 0.003 :t 0.0058** 0.0076 :t 0.0088** 2.2 :t 4.6** 0.84 :t 0.13 27 0.81 :t 1.6 2.1 :t 2.4 590 :t 1,200 230 :t 35 12 0.00036 :t 0.0017** 0.0069 :t 0.0078** 0.71:t 5.6** 1.3:t 0.18 26 0.094 :t 0.44 1.8 :t 2.0 180 :t 1,500 340 :t 47 13 0.0011 :t 0.0035** 0.012 :t 0.012** 2.4 :t 5.4** 1. 2 :t O. 17 28 (dupli- 0.31 :t 0.98 3.4 :t 3.4 670 :t 1,500 340 :t 48 ~ cate (.11 No. 12) 14 0.00056 :t 0.0026** 0.00056 :t 0.0026** 4.1 :t 4.4** 2.8 :t 1.4 39 0.22 :t 1.0 0.22 :t 1.0 1,600 :t 1,700 1,100 :t 550 15 0.0019 :t 0.0039** 0.0045 :t 0.0064** o. 73 :t 1. 2** 2.9 :t 0.4 28 0.53 :t 1. 1 1.3:t 1.8 200 :t 340 310 :t 110 Med i an 0.00083** 0.0063** 1.1** 1.4 28 0.27 1.4 310 340 Rang e 0.00031** - 0.0064** 0.00056** - 0.02** 1. 5** - 4.1** 0.55 - 2.9 15 - 39 0.063 - 2.2 0.18 - 3.6 -300 - 1,600 150 - 1,100 * Wet weight ** Counting error exceeds reported activity. ------- TABLE E-2. GAMMA-EMITTING RADIONUCLIDE AND TRITIUM CONCENTRATIONS IN KIDNEYS, DESERT BIGHORN SHEEP, 1980 K Cesium-137 Hydrogen-3 Ani mal Number (g/g*) (pCi/kg*) (pCi/l*) 1 13 + 0.78 <84 <420 2 17 + 1.1 <112 <420 3 14 + 1. 4 <123 <420 4 8 + 0.65 <51 <420 5 7.9 + 0.67 <93 <420 6 5.6 + 0.42 91 + 22 <330 7 11 + 0.64 <61 <420 8 6.1 + 0.45 <40 <330 9 8.6 + 0.69 <88 <330 10 6.1 + 0.39 31 + 18 <420 11 9.9 + 0.68 <80 <420 12 7.3 + 0.58 <50 <420 Median 8.3 <82 <420 Range 5.6 - 17 31 - <123 <330 - <420 * Wet weight + Aqueous portion of kidney tissue 46 ------- TABLE F-1. PLUTONIUM, STRONTlU~1, AND GAMMA-EMITTING RADIONUCLIDE CONCENTRATIONS IN SELECTED TISSUES, NTS HORSE, 1980 Plutonium-238 Pluton i um-239 Stront ium-89 Strontium-90 Tissue (pCi /g Ash) (pCi /g Ash) (pCi/g Ash) (pCi/g Ash) Ash Type (pCi /kg*) (pCi/kg*) (pCi/kg*) (pCi /kg*) (%) K (g/kg*) Bone -0.0012 I 0.004** 0.015 I 0.015 150 :!: 515** 3.5 I 0.5 27 NA -0.32 I 1.1** 3.9 I 3.9 40,000 I 140,000** 950 I 130 Mu sc 1 e 0.0031 I 0.0059** 0.0057 I 0.0081** NA NA 0.78 6.3 I 0.32 +:> 0.024 I 0.046** 0.044 I 0.063** -....J Liver 0.034 I 0.0088 0.98 I 0.11 NA NA 1.3 4.4 I 0.18 0.44 I 0.11 13 :!: 1.4 Lung 0.014 I 0.014 0.42 I 0.045 NA NA 1.1 7.2 I 0.57 0.15 IO.15 4.7 :!: 0.56 Thyrai d NA NA NA NA NA 24 I 2. 7 * Wet weight ** Counting error exceeds reported activity. NA Not Analyzed ------- TABLE G-1. PLUTONIUM CONCENTRATIONS IN SELECTED TISSUES, RABBITS, AREA 15, NTS, 1980 Plutonium-238 Plutonium-239 (pCi /g Ash) (pCi/g Ash) Ash An i ma 1 Number (p C i /k 9 *) (pCi/kg*) (%) Muscles 1 -0.0027 1: 0.0055** 0.0441:0.011 2.0 -0.053 1: 0.11** 0.88 1: 0.21 2 -0.0011 1: 0.0052** 0.018 1: 0.021** 1.4 -0.016 1: 0.073** 0.25 1: 0.3** 3 0.00 1: 0.0088** 0.037 1: 0.041** 1.6 0.001:0.14** 0.59 1: 0.65** 4 -0.0029 1: 0.0066** 0.051 1: 0.013 1.4 -0.04 1: 0.093** 0.71 1: 0.18 M ed i an -0.0019** 0.041 1. 5 -0.028** 0.65 Rang e -0.0029** - 0.00** 0.018** - 0.051 1.4 - 2 -0.053** - 0.00** 0.25** - 0.88 Internal Organs 1 0.031 1: 0.012 0.61 1: 0.079 1.4 0.43 1: 0.17 8.5 1: 1.1 2 Sample Lost 3 0.067 1: 0.028 0.93 1: 0.018 1.5 1.0 1: 0.42 14 1: 2.7 4 0.011 1: 0.016** 0.141:0.027 1.3 0.15 1: 0.21** 1.8 1: 0.35 Median 0.031 0.61 1.4 0.43 8.5 Range 0.011** - 0.067 0.14 - 6.93 1.3 - 1.5 0.15** - 1. 0 1. 8 - 14 (continued) 48 ------- TABLE G-1. (Cont i nued) Plutonium-238 Plutonium-239 Animal Number (pCi/g Ash) (pCi /g Ash) Ash (pCi/kg*) (pCi /kg*) (%) G. I. Tract 1 0.016 :!: 0.008 0.38 :!: 0.042 2.1 0.34:!: 0.12 7.9 :!: 0.88 2 0.038 :!: 0.01 0.88 :!: 0.096 2.4 0.9 :!: 0.25 21 :!: 2.3 3 1. 5 :!: O. 28 33 :!: 4.8 13 200 :!: 30 4,300 :!: 630 4 0.017 :!: 0.0063 0.63 :!: 0.063 1.9 0.32 :!: 0.12 12 ::!: 1. 2 Medi an 0.028 0.76 2.3 0.62 17 Range 0.016 - 1. 5 0.38 - 33 1.9 - 13 0.32 - 200 7.9 - 4,300 Skin 1 0.28:!: 0.057 10 :!: 0.95 2.2 6. 2 ::!: 1. 3 220 ::!: 21 2 0.33 ::!: 0.096 16 ::!: 2.3 2.4 8.0 ::!: 2.3 380 ::!: 56 3 0.46 :!: 0.079 19 ::!: 1. 8 2.2 10 ::!: 1. 8 420 ::!: 40 4 0.25 ::!: 0.052 7.8:!:0.74 2.3 5.8 ::!: 1.2 180 :!: 17 Median 0.31 13 2.3 7.1 300 Rang e 0.25 - 0.46 7.8 - 19 2.2 - 2.4 5.8 - 10 180 - 420 * Wet weight exceeds reported activity. ** Counting error 49 ------- TABLE G-2. PLUTONIUM AND STRONTIUM CONCENTRATIONS IN BONES, RABBITS, AREA 15, NTS, 1980 Plutonium-238 Plutonium-239 Strontium-89 Strontium-90 An i mal (pCi /g Ash) (pCi/g Ash) (pCi /g Ash) (pCi/g Ash) Ash Number (pCi /kg*) (pCi /kg*) (pCi/kg*) (pCi /kg*) (%) 1 -0.0012 ! 0.0039** 0.018! 0.0069 530 ! 830** 2.6 ! 0.38 12 -0.14 ! 0.48** 2.2 ! 0.86 66,000 ! 100,000** 320 ! 47 2 0.0 ! 0.0024** 0.02 ! 0.0071 170 ! 1,000** 3.5 ! 0.49 14 0.0 ! 0.36** 2.9 ! 1.0 24,000 ! 110,000** 500 ! 71 01 3 0.00065 ! 0.003** 0.049 ! 0.01 -140 ! 780** 2.4 ! 0.36 15 a 0.098 ! 0.46** 7.3 ! 2.0 -22,000 ! 110,000** 360 ! 55 4 0.0006 ! 0.0026** 0.042 ! 0.012 350 ! 470** 1.5 ! 0.22 14 0.08 ! 0.37** 6. 1 ! 1. 8 51,000 ! 68,000** 270 ! 32 Med i an .0003** 0.031 260** 2.5 14 0.04** 4.5 38,000** 340 Rang e -0.0012**- 0.00065** 0.018 - 0.049 -140** - 530** 1. 5 - 3.5 12 - 15 -0.14** - 0.098** 2.2 - 7.3 -22,000**- 66,000** 220 - 500 * Wet weight ** Counting error exceeds reported activity. ------- TABLE G-3. GAMMA-EMITTING RADIONUCLIDE AND TRITIUM CONCENTRATIONS IN SELECTED TISSUES, RABBITS, AREA 15, NTS, 1980 G. I. Tract Mu sc 1 es K (g/kg*) K (g/kg*) Interna 1 Organs 137CS(pCi/kg*) Skin Ani mal 137Cs(pCi /kg) K (g/kg*) 102mRh(pCi/kg*) K (g/kg*) Number 3H (pCi /1) 137Cs(pCi/kg*) 60Co(pCi/kg*) 137CS(pCi/kg) 1 9.2 :t 0.57 9.3 :t 0.3 4.7 :t 0.34 13 :t 1.3 98 :t 16 71 :t 14 63 :t 16 250 :!: 91 4,100 :t 310 <28 <28 2 5.7 :!: 0.43 5.9 :!: 0.45 6.3 :t 0.44 10 :t 1.6 130 :t 20 90 :t 23 150 :t 22 200 :t 120 1,200 :t 270 <42 <42 3 5.0 :t 6.34 4.6 :t 0.34 11 :t 0.56 4.9 :t 1. 3 82 :t 16 37 :!: 18 2,300 :t 61 140 :t 71 6,500 :t 340 130 :t 25 130 :!: 13 4 6.0 :t 0.37 3.9 :t 0.35 4.6 :t 0.34 15 :t 1. 6 88 :t 13 46 :t 16 33 :t 15 130 :t 87 5,300 :t 320 <29 <29 M ed i a n 5.9 5.3 5.5 11. 6 93 59 107 170 4,700 <36 <36 Range 5.0 - 9.2 3.9 - 9.3 4.6 - 11 4.9 - 15 82 - 130 37 - 99 33 - 2,300 130 - 250 1,200 - 6,500 <28 - 130 <28 - 130 * Wet weight 51 ------- TABLE H. GROSS* AND MICROSCOPIC PATHOLOGyt FOUND IN NECROPSIED ANIMALS, 1980 AREA 18 CATTLE 1 Necropsy findings: The lungs were emphysematous from agonal struggling. Metal fragments were found in the reticulum. Histopathological findings: No significant lesions noted. spermatogenesis in the testes. No act i ve Clinical diagnosis: Normal immature bull. 2 Necropsy findings: on skin. Adhesions between diaphragm and liver. Lice are present Histopathological findings: Sarcocyst observed in cardiac muscle. There is noderate hemosiderosis of the spleen. The skin shows mild superficial dermal mononuclear infiltrate, etiology undetermined. An incidental finding was scattered tubular mineral deposits in the kidneys. Clinical diagnosis: Normal lactating cow. 3 Necropsy findings: No g r os s 1 e s ion s not ed . Histopathological findings: No significant lesions noted. finding was diffuse fatty changes in the liver. An incidental Clinical diagnosis: Normal 2-week old female calf. 4 Necropsy findings: Cauliflower growth on left eye (squamous cell carcinoma). Lungs contain areas of emphysema and atelectasis probably resulting from agonal struggling. * As reported by senior author t As reported by Dr. Billy C. Ward, College of Veterinary Medicine, Mississippi State University, Starkville, Mississippi. 52 ------- Histopathological findings: Mild hemosiderosis of the spleen. Sarcocysts are found in the cardiac muscle and the skeletal muscle shows moderate fatty inf~ltration. The neoplastic mass on the cornea is composed of moderate undlfferentiated squamous cells which occasionally form pearls on the corneal surface. The only significant lesion is the presence of an ocular squamous cell carcinoma. Clinical diagnosis: Mature cow with beginning ocular squamous cell carcinoma. 5 Necropsy findings: The left eye was completely enucleated by a squamous cell carcinoma and secondary bacterial infections. Uterus contained a 3-month male fetus. Histopathological findings: Sarcocysts are scattered throughout the myocardium. The spleen shows moderate hemosiderosis. Clinical diagnosis: Mature cow with advanced ocular squamous cell carcinoma. 6 Necropsy findings: No gross lesions noted. Histopathological findings: Mild peribronchial lymphoid proliferation noted in the lungs. No evidence of active spermatogenesis in the testes. Clinical diagnosis: Normal 6-month old bull calf. NEVADA TEST SITE MULE DEER 1 Necropsy findings: Trauma associated with bullet passage through the lungs, antlers stunted and knobby. Histopathological findings: were autolyzed. No significant lesions noted. Tissue samples Clinical diagnosis: Normal mature male deer. 2 Necropsy findings: Mature doe in lactation. collision. Neck was broken. Histopathological findings: Foccal hemorrhage in l~ng. noted. Samples showed advanced post-mortem autolyslS. Trauma associated with vehicular No other lesions Clinical diagnosis: Normal mature lactating female deer. 53 ------- 3 Necropsy findings: gun. Histopathological findings: spermatogenesis in testes. Fractured vertebrae from syringe fired from Cap-Chur No significant lesions. No evidence of active 4 Clinical diagnosis: Normal immature male deer. Necropsy findings: Trauma associated with bullet passage through lungs. Histopathological findings: A few sarcocysts are observed in the myocardium and skeletal muscle. There are small mineralized foci in the pelvis of the kidney. No evidence of active spermatogenesis in the testes. Clinical diagnosis: Normal immature male deer- AREA 15 HORSE Necropsy findings: The liver and lung surfaces and parenchyma contained numerous spherical nodules 2-5 mm in diameter. These nodules were whitish yellow in color and very hard. The urinary bladder contained several hundred cubic centimeters of a thick pustular exudate. The same exudate was found in the pelvis of the left kidney. Thyroid was very dense and hard. Histopathological findings: There are multifocal calcific nodules scattered throughout the liver and lung parenchyma. These nodules are surrounded by dense fibrous connective tissue bands which occasionally have a few infiltrating inflammatory cells, many of which are eosinophils. The center of the nodule is composed of dense calcified tissue. There is some linear mineralization in the choroid behind the retinal epithelium. The thyroid displays marked irregularity of follicular size and of choloid content. The muscle shows some fatty infiltration. There are a variety of mild, probably age-associated lesions in the kidney. These consist of mild multifocal interstitial fibrosis with moderate accumulation of mononuclear cells associated with fibrotic lesions. There are some protein casts in dilated tubules and an occasional granular cast. Focal glomerular scarring is occasionally observed. Clinical diagnosis: Aged horse showing the degenerative changes of advanced age. The major findings in this horse include mineralization behind the retina, mild interstitial nephritis and degenerative changes of the kidney, and mild thyroid atrophy and regeneration. The multifocal calcific nodules in the liver and lungs are probably associated with old calcified parasitic 9 ranu lomas. 54 ------- AREA 15 RABBITS All of these animals were collected by shotgun fire. All were placed il1 a freezer until sampled. No gross lesions were noted by dissector. All were apparently normal at time of death. Samples were not collected for histopathological examination. DESERT BIGHORN SHEEP All of these animals were mature males that were killed by licensed hunters. Necropsies were not performed but all were apparently normal at time of death. Samples were not collected for histopathological examination. 55 ------- TECHNICAL REPORT DATA II (Please read Instructions on the reverse before completing) 12. 3. RECIPIENT'S ACCESSION NO. II 1. REPORT NO. 4. TITLE AND SUBTITLE 5. REPORT DATE ANIMAL INVESTIGATION PROGRAM 1980 REPORT ---- Nevada Test Site and Vicinity 6. PERFORMING ORGANIZATION CODE EP A/600/07 -- 7. AUTHOR(S) 8. PERFORMING ORGANIZATION REPORT NO' 1). D. Smith, K. R. Giles and D. E. Bernhardt 9. PERFORMING ORGANIZATION NAME AND ADDRESS 10. PROGRAM ELEMENT NO. Environmental Monitoring Systems Laboratory X6EHI0 Office of Research and Development 11. CONTRACT/GRANT NO. U. S. Envi ronmental Protection Agency IA Las Vegas, Nevada 89114 DE-AI08-76DP00539 -- 13. TYPE OF REPORT AND PERIOD COVERED 12. SPONSORING AGENCY NAME AND ADDRESS U. S. Depa rtment of Energy Cal encliJf 1 qgn ---- Nevada Operations Office 14. SPONSORING AGENCY CODE P.O. Box 14100 Las Vegas, Nevada 89114 -- 15. SUPPLEMENTARY NOTES Performed under Interagency Agreement DE-AI08-76DP00539 for the u.S. Department of Energy ----1 16.ABSTRACTUata are presented from the radioanalysis of tissues collected from animals! that resided on or near the Nevada Test Site (NTS). Gamma-emitting radionuclides were detected infrequently. Iodine-131 was found in the thyroids of a deer 3 weeks after a nuclear test by the People IS Republic of China. Strontium-90 and plutonium con centra- tions in NTS animal tissues were similar to those of recent years. Radionuclide tissue concentrations were generally higher in the tissues of animals residing in Area 15. Annual dose estimates to man were calculated based on the daily consumption of 0.5 kg of tissue with peak radionuclide levels. The highest postulated dose was 0.4 millirems to the whole body from consuming cattle muscle containing 37 pCi cesium-137 per kg. This dose is about 0.1 percent of the radiation protection guide. The movements of 26 mule deer fitted with collars containing a radio transmitter unit were monitored. By mi d-December, all deer had left their summer ranges. In general, deer captured on Pahute Mesa winter in the 40-Mile Canyon and Timber Mountain areas with some movement off the NTS toward Beatty, while those captured on Rainier Mesa winter in the Shoshone Mountain area. A deer captured in December 1979 was seen in July and August, approximately 160 kilometers from its capture point. NTS mule deer population estimates based on weekly sightings were made for 1978, 19 7 9 and 1980. No gross or microscopic lesions in necropsied animals were found that could be directly attributed to the effects of ion i zing radiation. 17. KEY WORDS AND DOCUMENT ANALYSIS a. DESCRIPTORS b.IDENTIFIERS/OPEN ENDED TERMS C. COSA TI Field/Group 18. DISTRIBUTION STATEMENT 19. SECURITY CLASS (This Report) 21. NO. OF PAGES UNCLASSIFIED RELEASE TO PUBLIC 20. SECURITY CLASS (TlJis page) 22. PRICE UNCLA<\SIFTFn EPA Form 2220-1 (Rev. 4-77) PREVIOUS EDITION IS OBSOLETE ------- |