EMSL-LV-0539-11 EMSL-LV-0539-11 STATUS REPORT OF AN EXPERIMENTAL DAIRY HERD MAINTAINED ON THE NEVADA TEST SITE JANUARY 1, 1976, THROUGH DECEMBER 31, 1976 Monitoring Systems Research and Development Division Environmental Monitoring and Support Laboratory U.S. ENVIRONMENTAL PROTECTION AGENCY Las Vegas, NV 89114 Published April 1978 This study was performed under a Memorandum of Understanding No. EY-76-A-080539 for the DEPARTMENT OF ENERGY ------- This report was prepared as an account of work sponsored by the United States Government. Neither the United States nor the Department of Energy, nor any of their employees, nor any of their contractors, subcontractors, or their employees, makes any warranty, express or implied, or assumes any legal lia- bility or responsibility for the accuracy, completeness or usefulness of any information, apparatus, product or process disclosed, or represents that its use would not infringe privately owned rights. AVAILABLE FROM THE NATIONAL TECHNICAL INFORMATION SERVICE U.S. DEPARTMENT OF COMMERCE SPRINGFIELD, VA 22161 PRICE: PAPER COPY $4.50 MICROFICHE $3.00 ------- EMSL-LV-0539-11 EMSL-LV-0539-11 STATUS REPORT OF AN EXPERIMENTAL DAIRY HERD MAINTAINED ON THE NEVADA TEST SITE JANUARY 1, 1976, THROUGH DECEMBER 31, 1976 by E. M. Daley Monitoring Systems Research and Development Division Environmental Monitoring and Support Laboratory U.S. ENVIRONMENTAL PROTECTION AGENCY Las Vegas, NV 89114 Published April 1978 This study was performed under a Memorandum of Understanding No. EY-76-A-080539 for the DEPARTMENT OF ENERGY ------- ABSTRACT The Environmental Monitoring and Support Laboratory-Las Vegas, U.S. Envi. ronmental Protection Agency, maintains an experimental dairy herd and farm facility in Area 15 of the Nevada Test Site for the U.S. Energy Research and Development Administration*. This status report covers the period from January 1, 1976, through December 31, 1976. Improvements, changes, and addi- tions made to the facilities, production and reproduction statistics for individual cows and the herd, the veterinary medicine practices employed, and summaries of the metabolism studies that involved the dairy herd are covered in this report. *Effective October 1, 1977, the U.S. Energy Research and Development Administration was designated the Department of Energy. ii ------- TABLE OF CONTENTS ABSTRACT INTRODUCTION FACILITIES AND EQUIPMENT Milking Parlor Holding Pen and Alleyway Road and Parking Lot Miscellaneous HERD MANAGEMENT AND PRODUCTION Production Reproduction VETERINARY MEDICINE DAIRY HERD INVOLVEMENT IN EXPERIMENTS Biological Transport of Curium-243 in Lactating Cows: Project Curium (June 2-9, 1976) REFERENCES APPENDICES DISTRIBUTION Hi Page iii 1 3 3 5 5 5 6 7 7 8 9 Dairy 9 11 ------- INTRODUCTION The Environmental Monitoring and Support Laboratory-Las Vegas (EMSL-LV) of the U.S. Environmental Protection Agency (EPA) has maintained an experi- mental dairy herd and farm for the U.S. Energy Research and Development Administration* since 1964. The dairy herd and farm facilities are located in Area 15 of the Nevada Test Site (see figure 1). This report covers the period of January 1, 1976, through December 31, 1976. It lists changes and improvements made on the facilities, presents herd and individual production, and reproduction statistics, details health problems and veterinary practices, and summarizes research studies that involved the dairy herd. Reports on the establishment of the experimental farm and the periodic status reports on the dairy herd have been published previously (Douglas 1967, Smith and Engel 1969. Smith 1970 and 1973, and Daley 1977). Reports on the agronomic practices employed at the farm were also published previously (Daley and Smith 1969. and Daley 1971, 1972, and 1976). *Effective October 1, 1977, the U.S. Energy Research and Development Administration was designated the Department of Energy. Prior to January 19, 1975, the U.S. Energy Research and Development Administration was designated as the u.S. Atomic Energy Commission. 1 ------- ..r----~ " , \---~ "'1 \' , j, o \ \ \ --- ----, I , EXPERIMENTAL FARM ---~ SEDAN CRATER ---j , I l' ---I I 1 31 -..----!J I i_J SCALE IN FEET ~.... I 1cx)()() 0 3:XXX) SCALE IN ~ o 5000 Figure 1. Location of the experimental farm and dairy herd on the Nevada Test Site. 2 o (1) (II (1) :1- (j) Iť 3 (1) r Iť (II < (1) (() Iť (II ffi 3 AI Iť :J (() (1) (1) (II ... o 3 ~ (1) , o c: , ~ ------- FACILITIES AND EQUIPMENT During the period covered by this report, major modifications and addi- tions were made to the Area 15 facility to increase the efficiency and safety of milking the cows. figure 2. These changes are discussed below and are shown in MILKING PARLOR The existing four-stanchion milking parlor was converted to a four-stall wa1k-through-pit milking parlor, as shown in the barn layout in figure 2. Variations of this type of milk parlor are commonly used in the Great Basin area. The design is such that there are two in-line stalls on each side of a work area pit. The concrete bases for the stalls were elevated about 10 inches (25 cm) above the existing floor. The stalls are made of l~-inch (3.B-cm) galvanized pipe secured with Kee K1amp@ fittings, and have stainless steel sliding doors. The stalls are secured to concrete bases with bolts embedded in the concrete. An excavation 27 inches (67.5 cm) deep, 54 inches (135 cm) wide, and 13 feet (5.59 m) long was made in the existing floor to provide the working pit area. The existing drain from the barn to the liquid disposal tank was modified to accommodate the newly installed drain in the south end of the pit. installed in the pit. Two DeLava1 milkers with PyreX@ scale jars were The jars have a graduated scale which indicates the milk production in pounds. One milking machine is located for use in milking the cows in the forward stalls, and another is located for use in milking the cows in the rear stalls. An anti-kicking device that restrains a problem cow against the stall side was designed by EMSL-LV Farm and Animal Investigation Branch personnel and incorporated into the stalls. @ Registered trademark 3 ------- METABOLISM ROOM ABATT:l SINK e DRUG ROOM SHOWER TRANSFORMER CHANGE MILKING PARLOR ROOM ~ MILK ROOM WASHING PEN CALF SHELTER CONCRETE APRON CALF PEN EXPERIMENTAL DAIRY FARM BARN FLOOR PLAN Figure 2. Experimental farm barn layout. 4 ------- The design of the new milking parlor allows workers to more closely observe the milking processes and to more readily determine when a cow's udder has been drained of the stored milk. Because the cows are more restricted in their movements while in the stalls, there is less danger to the operator of being kicked or trampled. All of the interior walls of the milking parlor and office were repainted and surfaced with a 4-foot (1.24-cm) high section of waterproof Miralite@ wall board. In areas of heavy water usage, the wall board was extended from the floor to the ceiling. HOLDING PEN AND ALLEYWAY A holding pen and an alleyway for the movement of the cows into the milking parlor were constructed. The pen and alleyway were constructed of metal panels suspended from posts made from well casing. To prevent slipping, both the pen and alleyway have a rough broom-finished concrete floor. This pen and alleyway arrangement allows a smoother, cleaner, and more efficient flow of cattle from the holding pen into the milking parlor. ROAD AND PARKING LOT During the fall of 1976, the access road into the farm and the parking lot in front of the barn were resurfaced with hot batch asphalt pavement. @Registered trademark 5 ------- HERD MANAGEMENT AND PRODUCTION The dairy herd consists of a group of about 35 purebred Holstein cows which are maintained in the lots of the Area 15 experimental farm. The vital statistics for all cows in the dairy herd during 1976 are listed in appendix A. During the growing season, usually from mid-April to early October, all forage is provided as greenfeed (freshly cut alfalfa or an alfalfa-grass mixture). During the rest of the year, the herd is maintained on alfalfa hay. All greenfeed forage is produced on the farm while most of the hay is purchased. Lactating cows are fed a commercial concentrate of l4-percent protein after each milking. Dairy practices, including milking procedures, follow those commonly used by producers in the Great Basin area, with two exceptions. The cows are milked on a schedule of 8- and l6-hour intervals (actual time 7 a.m. and 3 p.m.) rather than the traditional l2-hour schedule so that labor require- ments are reduced. Also, weight-measure jars are used and emptied after each cow is milked, instead of using a pipeline and holding tank system; this is done so that each cow's milk production may be recorded and to allow for the collection of individual milk samples. Ten Holstein cows were added to the dairy herd during January 1976. Five Holstein heifers in advanced stages of gestation were purchased from Paul Santos of Yerington, Nevada. An additional five pregnant young Holstein cows were purchased from Dante Lommari of Yerington, Nevada. These animals all calved between January and May. The entry of these cows into the herd caused no problems. The animals purchased were selected to meet the criteria for metabolism studies; these criteria included medium size, above average production, and gentle temperament. The purchase of these cows was necessary because previous terminal studies had eliminated suitable high producing 6 ------- cows from the herd, and some of the heifers kept for replacement animals failed to develop into high milk producing cows (see appendix B). At the end of 1976, the herd consisted of 24 mature cows. During the year, 11 cows deemed unsatisfactory were removed from the herd and 2 cows were sacrificed during experiments. PRODUCTION Appendix B shows that the average milk production per cow for the year was 4,013 kilograms (8,829 pounds) for the average 254-day lactation period. The extrapolated herd average for a 305-day lactation period would be 4,454 kilograms (9,800 pounds) of milk per cow or 14.6 kilograms (32.1 pounds) per day. This is slightly lower than the national average of 4,658 kilograms (10,271 pounds) of milk per cow (Dairy Illustrated 1976). However, several of the best producers in the herd had their lactations cut short by inclusion in terminal studies, or were culled from the herd because their size, age, or temperament made them unsuitable for use in metabolism studies. REPRODUCTION During the year 1976, 30 calves were born in the experimental dairy herd (see appendix C). Eighteen of the calves were males and 12 were females. Of the 30 calves, 2 purebred Holstein heifers were retained as replacements, 7 calves were transferred to the University of Nevada, Reno, 2 calves were stillborn, and 19 calves were euthanized because they were not needed in the program. Two sets of twins were born during 1976. The dam of one set was born in the herd and the dam of the other set was purchased. 7 ------- VETERINARY MEDICINE A preventive medicine program and a sanitation program were conducted to decrease the incidence of preventable disease. This included sanitizing the milking equipment after milking each cow plus dipping the teats in an antiseptic solution after milking to control the spread of mastitis, and routine palpation of the reproductive tract with indicated treatments for control of reproductive diseases. Magnets were used routinely to help prevent traumatic reticulitis (hardware disease). The magnets were introduced into the reticulum of the cow to help prevent penetration of critical organs by attracting and holding ferrous metals (i.e., nails, wire, etc.). During the greenfeed season, poloxalene blocks were fed free choice to control tympanites (bloat). No contagious diseases were diagnosed in 1976. causes during this period. No cows died from natural When possible, unproductive cows and cows that failed to respond to treat- , ment were salvaged by using them for terminal studies involving technique development, or were transferred to other agencies. As shown in appendix A, 11 cows were transferred to the University of Nevada, Reno, and 2 were used in terminal studies. 8 ------- DAIRY HERD INVOLVEMENT IN EXPERIMENTS During 1976, part of the dairy herd was used for a curium metabolism study described below. This study was one of a continuing series of studies to define the critical metabolic pathways of selected radionuclides which might appear in the environment following a nuclear detonation, during the reprocessing of nuclear reactor fuels, or as a result of an accident at a nuclear site. The cows for these experiments were selected on the basis of milk production, stage of lactation, temperament, soundness of legs and feet, and general health. The animals were put into special metabolism stalls which provided continuous restraint of the cow for milking and sampling while allow- ing sufficient movement for comfort. An indwelling catheter was passed through the urethra and into the cow's bladder and the total urine production was collected in a plastic bottle for the duration of the experiment. Feces were collected in a tray, lined with plastic sheeting, which was placed under a metal grate in the floor at the rear of the stall. sampling procedures were described by Stanley et al. The details of these (1974). BIOLOGICAL TRANSPORT OF CURIUM-243 IN LACTATING DAIRY COWS: PROJECT CURIUM (June 2-9, 1976) The objectives of this study were to obtain information on the blood:milk transfer and the blood:tissue transfer of curium in a ruminant and to assess the degree of bovine gastrointestinal absorption, tissue retention, and rate of excretion following curium administration. Plutonium and curium metabolism in the cow were compared for relative hazard values. Two lactating dairy cows (nos. 256 and 344) were maintained in metabolism stalls for 7 days. One animal received an acute oral dose of approximately 5 millicuries of curium-243. The other cow received single intravenous dose of approximately 500 microcuries of curium-243. Milk, blood, urine, and fecal samples were taken every 12 hours over a 6-day period after dosing. The cows 9 ------- were sacrificed on the seventh day after dosing. Tissue and blood samples were collected to establish the tissue:p1asma ratios. The results of the study for the single intravenous dose were presented at the Nevada Applied Ecology Group's Plutonium Information Conference, held March 3-4, 1977, in Las Vegas, Nevada (Patzer e1 a1., to be published). The curium levels in many of the samples from the orally dosed cow were below detectable levels from gamma-ray analysis. The data from radiochemistry analysis were not available at the time this report was prepared. 10 ------- REFERENCES Dairy Illustrated. "News for Dairymen." Dairy Illustrated 8(1):3 Spring 1976 Daley, E. M., and D. D. Smith. Agronomic Aspects of the Experimental Dairy Farm - January 1966 - December 1968. SWRHL-63r. U.S. Department of Health, Education, and Welfare, Southwestern Radiological Health Labora- tory, Las Vegas, Nevada. 41 pp. 1969 Daley, E. M. Agronomic Aspects of the Experimental Dairy Farm Durin~ 1969. SWRHL-104r. U.S. Department of Health, Education, and Welfare, South- western Radiological Health Laboratory, Las Vegas, Nevada. 19 pp. 1971 Daley, E. M. Agronomic Aspects of the Experimental Dairy Farm 1970. SWRHL- l17r. U.S. Department of Health, Education, and Welfare, Southwestern Radiological Health Laboratory, Las Vegas, Nevada. 18 pp. 1972 Daley, E. M. Agronomic Practices of the Nevada Test Site Experimental Dairy Farm During 1971, 1972, and 1973. EMSL-LV-539-5. U.S. Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Las Vegas, Nevada. 13 pp. 1976 Daley, E. M. Five-Year Summary Report of an Experimental Dairy Herd Main- tained on the Nevada Test Site 1971 Through 1975. EMSL-LV-0539-9. U.S. Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Las Vegas, Nevada. 40 pp. 1977 Douglas, R. L. Status of the Nevada Report for July 1964 - December Health, Education, and Welfare, Laboratory, Las Vegas, Nevada. Test Site Experimental Farm - Summary 1965. SWRHL-36r. U.S. Department of Southwestern Radiological Health 30 pp. 1967 Patzer, R. G., W. W. Sutton, A. A. Mullen, P- B. Hahn, and G. D. Potter. "Comparisons of Curium-243 and Plutonium-238 Biological Transfer in Dairy Animals Following- Intravenous Injections." Presented at the Nevada Applied Ecology Group's Plutonium Information Conference held March 3-4, 1977, in Las Vegas, Nevada. (to be published in NVO-18l) Smith, D. D., and R. E. Engel. Progress Report for the Bioenvironmental Research May 22, 1964, Through July 1, 1966. Part I. Experimental Dairy Herd. SWRHL-55r. U.S. Department of Health, Education, and Welfare, Southwestern Radiological Health Laboratoryo Las Vegas, Nevada. 106 pp. 1969 11 ------- Smith, D. D. Status of the Bioenvironmental Research Experimental Dairy Herd July 1, 1966, Through December 31, 1968. SWRHL-67r. U.S. Department of Health, Education, and Welfare, Southwestern Radiological Health Labora- tory, Las Vegas, Nevada. 90 pp. 1970 Smith, D. D. Status of the Environmental Protection Agency's Nevada Test Site Experimental Dairy Herd January 1, 1969 - December 31, 1970. NERC-LV- 539-22. U.S. Environmental Protection Agency, National Environmental Research Center, Las Vegas, Nevada. 62 pp. 1973 Stanley, R. E., E. W. Bretthauer, and W. W. Sutton. "Absorption, Distribution, and Excretion of Plutonium by Dairy Cattle." pp. 163-185. NVO-142. The Dynamics of Plutonium in Desert Environments, Nevada Applied Ecology Group Progress Report, July 1974. U.S. Atomic Energy Commission, Nevada Operations Office, Las Vegas, Nevada. P. B. Dunaway and M. G. White, eds. 369 pp. 1974 12 ------- APPENDIX A. VITAL STATISTICS AND ACQUISITION DATA OF MATURE DAIRY ANIMALS JANUARY 1, 1976 - DECEMBER 31, 1976 Cow Date of Birth Weight2 Acquisition No. Agel Type of Breeding Data Remarks 127 April 24, 1968 582 kg Born in herd. Transferred to 8 years 3 months Registered cow Dam was No. 62. UNR3 June 23, Sire was No. 70. 1976. 153 February 25, 1969 764 kg Born in herd. Transferred to 7 years 4 months Grade cow Dam was No. 71. UNR3 June 23, Sire was No. 70. 1976. 184 February 11, 1970 677 kg Born in herd In herd. 6 years 10 months Grade cow Dam was No. 117 - Sire was H57. 197 July 1, 1970 756 kg Born in herd. Transferred to 6 years Grade cow Dam was No. 127. UNR3 June 23, Sire was No. 126. 1976. 198 July 1, 1970 795 kg Born in herd. Transferred to 6 years Grade cow Dam was No. 45. UNR3 June 23, Sire was No. 126. 1976. 208 December 17, 1970 713 kg Born in herd. Transferred to 6 years 6 months Grade cow Dam was No. 39. UNR3 June 23, AI4 Sire was H290. 1976. 209 December 28, 1970 707 kg Born in herd. Transferred to 6 years 8 months Grade cow Dam was No. 21. UNR3 September 3, Sire was No. 126. 1976. 211 February 18, 1971 702 kg Born in herd. In herd. 5 years 10 months Grade cow Dam was No. 48. AI4 Sire was H322. 218 May 18, 1971 768 kg Born in herd. Transferred to 5 years 7 months Grade cow Dam was No. 122. UNR3 June 23, AI4 Sire was H290. 1976. A-I ------- APPENDIX A. CONTINUED Cow Date of Birth Weight2 Acquisition No. Agel Type of Breeding Data Remarks 221 June 17, 1971 624 kg Born in herd. In herd. 5 years 6 months Grade cow Dam was No. 139. Sire was No. 126. 227 September 6, 1971 807 kg Born in herd. In herd. 5 years 3 months Grade cow Dam was No. 162. Sire was No. 126. 241 December 22, 1971 72 0 kg Born in herd. In herd. 5 years Grade cow Dam was No. 128. AI4 Sire was H290. 250 July 3, 1972 527 kg Born in herd. In herd. 4 years 5 months Grade cow Dam was No. 83. AI4 Sire was H290. 251 July 3, 1972 598 kg Born in herd. In herd. 4 years 5 months Grade cow Dam was No. 83. AI4 Sire was H290. 252 July 8, 1972 630 kg Born in herd. Transferred to 3 years 11 months Grade cow Dam was No. 153. UNR3 June 23, AI4 Sire was H290. 1976. 256 August 7, 1972 540 kg Born in herd. Sacrificed 3 years 10 months Grade cow Dam was No. 196. Curium Project AI4 Sire was H290. June 9, 1976. 269 November 7, 1972 504 kg Born in herd. In herd. 4 years 1 month Grade cow Dam was No. 197. Sire was No. 216. 278 July 1, 1973 682 kg Born in herd. Transferred to 2 years 11 months Grade cow Dam was No. 184. UNR3 June 23, Sire was No. 216. 1976. 280 July 6, 1973 602 kg Born in herd. In herd. 3 years 5 months Grade cow Dam was No. 196. Sire was No. 216. A-2 ------- APPENDIX A. CONTINUED Cow Date of Birth Weight2 Acquisition No. Agel Type of Breeding Data Remarks 281 July 8, 1973 630 kg Born in herd. In herd. 3 years 5 months Grade cow Dam was No. 135. Sire was No. 216. 284 August 15, 1973 534 kg Born in herd. Transferred to 2 years 10 months Grade cow Dam was No. 83. UNR3 June 23, Sire was No. 216. 1976. 292 November 28, 1973 650 kg Born in herd. In herd. 3 years 1 month Grade cow Dam was No. 128. Sire was No. 216. 294 December 6, 1973 555 kg Born in herd. Transferred to 3 years Grade cow Dam was No. 221. UNR3 Septem- Sire was No. 216. ber 3, 1976. 297 January 16, 1974 623 kg Born in herd. In herd. 2 years 11 months Grade cow Dam was No. 198. Sire was No. 216. 299 March 18, 1974 573 kg Born in herd. In herd. 2 years 9 months Grade cow Dam was No. 123. Sire was H290. 314 December 3, 1974 432 kg Born in herd. In herd. 2 years Grade cow Dam was No. 250. Sire was No. 216. 315 December 7, 1974 420 kg Born in herd. In herd. 2 years Grade cow Dam was No. 197. Sire was No. 216. 344 Born 1973 659 kg Purchased D. Sacrificed 'v 3 years Grade cow Lommari $600.00. Curium Project Yerington, NV. June 9, 1976. 345 February 1973 520 kg Purchased D. In herd. 'v 3 years Grade cow Lommari $600.00. Yerington, NV. A-3 ------- APPENDIX A. CONTINUED Cow Date of Birth Weight2 Acquisition No. Agel Type of Breeding Data Remarks 346 January 1974 495 kg Purchased P. In herd. ,\., 2 years Grade cow Santps $600.00. Yerington, NV. 347 Fall 1974 468 kg Purchased P. In herd. '\., 2 years Grade cow Santos $600.00. Yerington, NV. 348 February 1974 427 kg Purchased P. In herd. '\., 2 years Grade cow Santos $600.00. Yerington, NV. 349 February 1974 507 kg Purchased P. In herd. '\., 2 years Grade cow Santos $600.00. Yerington, NV. 350 January 1974 511 kg Purchased P. In herd. ,\., 2 years Grade cow Santos $600.00. Yerington, NV. 351 March 1973 664 kg Purchased D. In herd. '\., 3 years Grade cow Lornrnari $600.00. Yerington, NV. 352 January 1973 507 kg Purchased D. In herd. '\., 3 years Grade cow Lornrnari $600.00. Yerington, NV. 353 February 1973 545 kg Purchased D. In herd. '\., 3 years Grade cow Lornrnari $600.00. Yerington, NV. lAge as of December 31, 1976, or death, or disposition. 2To convert to pounds: 2.205 x kilograms = pounds. 3UNR = University of Nevada, Reno. 4AI = artificial insemination. A-4 ------- APPENDIX B. MILK PRODUCTION OF COWS COMPLETING LACTATION DURING 1976 Cow Production Lactation No. (kg) 1 (days) From To 1272 2,660 151 01/24/76 06/23/76 1532 2,098 883 03/28/76 06/23/76 184 3,889 284 12/08/75 09/17/76 1982 3,706 253 07/27/75 04/04/76 2092 792 533 07/03/76 08/25/76 211 1,765 126 09/03/75 01/07/76 211 370 373 08/19/76 09/25/76 2182 1,331 993 03/15/76 06/23/76 227 5,325 308 01/02/76 11/05/76 241? 6,752 309 12/19/75 10/23/76 256t< 2,738 166 12/27/75 06/09/76 280 4,436 276 11/27/75 08/28/76 281 3,183 295 11/28/75 09/17/76 2842 256 513 12/09/75 01/29/76 294 20 63 OS/23/76 05/30/76 3444 3,098 158 01/03/76 06/09/76 345 5,970 310 01/13/76 11/19/76 B-1 ------- APPENDIX B. CONTINUED Cow Production Lactation From To No. (kg) 1 (days) 346 4,703 305 02/09/76 12/10/76 348 2,669 212 02/18/76 09/17/76 349 4,830 308 02/06/76 12/10/76 350 4,299 305 02/18/76 12/19/76 352 4,187 304 02/10/76 12/10/76 AVERAGES 4,013/year/cow 254/cow 16/day/cow 1To convert to pounds: 2.205 x kilograms = pounds. 2Transferred to University of Nevada, Reno. . 3Not included in average producing less than 100 days. 4Sacrificed 5Does not include cows that lactated less than 100 days. B-2 ------- APPENDIX C. CALVES BORN IN THE HERD DURING 1976 Calf Dam Sire Birth No. No. ID. Date Sex Remarks 336 227 518 01/01/76 F Crossbred Hereford. Euthanized as not needed in program. 337 256 H919 01/09/76 F In herd as kept for replacement. (Numbers 338 through 343 were used on calves purchased for an experiment. Numbers 344 through 353 were used on heifers and cows brought in Yerington, Nevada.) --- 1 345 Lommari bull 01/13/76 F Crossbred. Euthanized as not needed in program. --- 1 344 Lommari bull 01/17/76 M Crossbred. Euthanized as not needed in program. 354 127 H919 01/24/76 M Euthanized as not needed in program. 355 349 Santos bull 02/05/76 M Crossbred. Transferred to UNR2 on September 3, 1976. 356 346 Santos bull 02/08/76 F Crossbred. Stillbirth. 357 352 Lommar i bull 02/08/76 M Crossbred. Transferred to UNR2 on September 3, 1976. 358 348 Santos bull 02/17/76 F Crossbred. Transferred to UNR2 on September 3, 1976. 359 350 Santos bull 02/18/76 M Crossbred. Transferred to UNR2 on September 3, 1976. 360 347 Santos bull 03/06/76 M Crossbred. Stillbirth. 361 218 517 03/06/76 F Crossbred Hereford. Transferred to UNR2 on September 3, 1976. C-1 ------- APPENDIX C. CONTINUED Calf Dam Sire Birth No. No. ID. Date Sex Remarks 362 153 517 03/27/76 M Crossbred Hereford. Transferred to UNR2 on September 6. 1976. 363 299 517 04/08/76 M Crossbred Hereford. Transferred to UNR2 on September 6. 1976. 364 297 517 04/12/76 F Crossbred Hereford. Euthanized as not needed in program. 365 292 517 04/22/76 M Crossbred Hereford. Euthanized as not needed in program. 366 250 517 OS/21/76 M Twin of 367. Crossbred. Euthanized as not needed in program. 367 250 517 OS/21/76 M Twin of 366. Crossbred. Euthanized as not needed in program. 368 294 517 OS/23/76 M Crossbred. Euthanized as not needed in program. 369 353 Lommari bull OS/29/76 M Crossbred. Euthanized as not needed in program. 370 351 Lommari bull 06/06/76 M Twin of 371. Euthanized as not needed in program. 371 351 Lommari bull 06/06/76 F Twin of 370. Euthanized as not needed in program. 372 209 517 07/02/76 F Crossbred. Euthanized as not needed in program. 373 211 517 08/12/76 M Crossbred. Euthanized as not needed in program. 374 269 517 09/19/76 M Crossbred. Euthanized as not needed in program. 375 221 517 09/03/76 M Crossbred. Euthanized as not needed in program. C-2 ------- APPENDIX C. CONTINUED Calf Dam Sire Birth No. No. ID. Date Sex Remarks 376 251 517 10/11/76 F Crossbred. Euthanized as not needed in program. 377 315 H919 11/20/76 M Euthanized as not needed in program. 378 280 517 11/23/76 F Crossbred. Euthanized as not needed in program. 379 314 H341 12/11/76 F Kept for replacement. "lNo number assigned 2UNR = University of Nevada, Reno. C-3 ------- 1 - 40 41 42 43 46 - 47 48 - 49 50 - 51 56 57 - 58 59 60 61 62 63 - 67 DISTRIBUTION Environmental Monitoring and Support Laboratory--Las Vegas Mahlon E. Gates, Manager, DOE/NV, Las Vegas, NV 44 45 Troy E. Wade, DOE/NV. Las Vegas, NV David G. Jackson, DOE/NV, Las Vegas, NV Paul J. Mudra, DOE/NV, Las Vegas, NV Elwood M. Douthett, DOE/NV, Las Vegas, NV Ernest D. Campbell, DOE/NV, Las Vegas, NV Paul B. Dunaway, DOE/NV, Las Vegas, NV 52 53 54 55 Mary G. White, DOE/NV, Las Vegas, NV Roger Ray, DOE/NV, Las Vegas, NV Robert W. Taft, DOE/NV, Las Vegas, NV Leon Silverstrom, DOE/NV, Las Vegas, NV Robert W. Newman, DOE/NV, Las Vegas, NV Bruce W. Church, DOE/NV, Las Vegas, NV Technical Library, DOE/NV, Las Vegas, NV Chief, NOB/DNA, DOE/NV, Las Vegas, NV Hal Hollister, DSSC, DOE/HQ, Washington, DC Tommy F. McCraw, DOS, DOE/HQ, Washington, DC L. Joe Deal, DOS, DOE/HQ, Washington, DC Major General Joseph K. Bratton, Asst. Gen. Mgr., DMA 68 69 DOE/HQ, Washington, DC Gordon F. Facer, DMA, DOE/HQ, Washington, DC James L. Liverman, Director, DBER, DOE/HQ, Washington, DC Robert L. Watters, DBER, DOE/HQ, Washington, DC John S. Kirby-Smith, DBER, DOE/HQ, Washington, DC Jeff Swinebroad, DBER, DOE/HQ, Washington, DC 70 71 72 73 Robert W. Wood, DBER, DOE/HQ, Washington, DC William S. Osburn, Jr., DBER, DOE/HQ, Washington, DC 74 75 76 Ray Brechbill, DOE/SAN, Oakland, CA Marcie Williamson, HSL/INEL, DOE/ID, Idaho Falls, ID Steven V. Kaye, Oak Ridge National Lab., Oak Ridge, TN 77 ------- 97 98 99 100 101 102 103 104 105 106 107 108 109 78 79 80 Helen Pfuderer, ESIC, Oak Ridge National Lab., Oak Ridge, TN P. Wahlgren, CARL, Oak Ridge National Lab., Oak Ridge, TN H. E. Walburg, CARL, Oak Ridge National Lab., Oak Ridge, TN Asst. Admin. for Research and Development, EPA, Washington, DC 81 82 Deputy Asst. Admin. for Radiation Programs, EPA, Washington, DC Director, Div. of Criteria and Standards, ORP, EPA, Washington, DC 83 84 85 Director, Div. of Field Operations, ORP, EPA, Washington, DC Director, Div. of Technology Assessment, ORP, EPA, Washington, DC 86 87 Director, Office of Technical Analysis, EPA, Washington, DC Library, EPA, Washington, DC Paul DeFalco, Jr., Regional Administrator, Region IX, EPA, San Francisco, CA 88 89 James K. Channell, Regional Radiation Representative, Region IX, EPA, San Francisco, CA Richard L. Blanchard, Director, Radiochemistry and Nuclear Engineering Branch, EPA, Cincinnati, OH 90 91 Charles R. Porter, Director, Eastern Environmental Radiation Facility, EPA, Montgomery, AL Peter Halpin, Chief, APTIC, EPA, Research Triangle Park, NC 92 93 94 95 96 Harold F. Mueller, ARL, NOAA, Las Vegas, NV Gilbert J. Ferber, ARL, NOAA, Silver Spring, MD K. M. Oswald, Mgr., Health and Safety, LLL, Mercury, NV Bernard W. Shore, LLL, Livermore, CA Richard L. Wagner, LLL, Livermore, CA Howard W. Tewes, LLL, Livermore, CA Paul L. Phelps, LLL, Livermore, CA Mortimer L. Mendelsohn, LLL, Livermore, CA J. C. Hopkins, LASL, Los Alamos, NM Harry S. Jordan, LASL, Los Alamos, NM Lamar J. Johnson, LASL, Los Alamos, NM George E. Tucker, Sandia Laboratories, Albuquerque, NM Carter D. Broyles, Sandia Laboratories, Albuquerque, NM Melvin L. Merritt, Sandia Laboratories, Albuquerque, NM R. Glen Fuller, Oracle, AZ Richard S. Davidson, Battelle Memorial Institute, Columbus, OH Arden E. Bicker, REECo, Mercury, NV ------- 110 III 112 113 114 115 116 117 118 119 127 128 - 155 Savino W. Cavender, REECo, Mercury, NV Auda F. Morrow, CETO, NTS, Mercury, NV Billy Moore, NTSSO, DOE /NV, Mercury, NV Leo Bustad, Director, Veterinary Medicine, Washington State University, Pullman, WA Vincent Schultz, Washington State University, Pullman, WA Arthur Wallace, University of California, Los Angeles, CA Wesley E. Niles, University of Nevada, Las Vegas, NV Library. University of Nevada, Las Vegas, NV Verle R. Bohman, University of Nevada, Reno, NV 120 Lloyd P. Smith, President, Desert Research Institute, University of Nevada, Reno, NV Paul R. Fenske, Desert Research Institute, University of Nevada, Reno, NV 121 Thomas P. O'Farrell, Director, Applied Ecology and Physiology Center, Desert Research Institute, Boulder City, NV William S. Twenhofel, U.S. Geological Survey. Denver, CO 122 123 Manager, Desert National Wildlife Range, U.S. Fish and Wildlife Service, Las Vegas, NV Supervisor, Region III, Nevada Fish and Game Department, Las Vegas, NV 124 125 of Archives, Paul Lyons, Nevada Wildlife Research, Division Capitol Building Annex, Carson City, NV Deward W. Efurd, McClellan Central Laboratory, Air Force Base, CA L. L. Skolil, San Diego State University, San Diego, CA 126 McClellan Technical Information Center, ERDA, Oak Ridge, TN (for public availability) ------- |