U.S. VEPttTMENT OF COMMERCE
National Technical Infomutioa Stnrict
PB-265 040
Algal Nutrient Availability & Limitation in Lake Ontario
during IpYGL. Appendices to Part I. Available Phosphorus
in Urban Runoff and Lake Ontario Tributary Waters
Texas Univ. at Dallas, Richardson. Ctr for Environmental Studies
Prepared for
Environmental Research Lab, Duluth, Minn.
Dec 76
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EPA-600/3-76-094b
1976
ALGAL NUTRIENT AVAILABILITY AND LIMITATION
IN LAKE ONTARIO DURING IFYGL
Appendices Co
Part I. Available Phosphorus in Urban Runoff
and Lake Ontario Tributary Waters
by
William F. Cowen
and
G. Fred Lee
University of Texas at Dallas
Richardson, Texas 75080
Contract No. R-800537-02
Project Officer
Nelson Thomas
Large Lakes Research Station
Environmental Research Laboratory-Duluth
Grosse lie, Michigan 48138
ENVIRONMENTAL RESEARCH LABORATORY-DULUTH
OFFICE OF RESEARCH AND DEVELOPMENT
U.S. ENVIRONMENTAL PROTECTION AGENCY
DULUTH, MINNESOTA 55804
-------
TECHNICAL REPORT DATA
(Pteau nod /ttstrvctiont on the revcne before completing)
1. REPORT. NO.
EPA-600/3-76-094b
2.
3. RECIPIENT'S ACCESSION*NO.
«. TITLE AND SUBTITLE ^g^ wnKLESI AVAILABILITY AND LIMITA-
TION IN LAKE ONTARIO DURING IFYGL. Appendices to
Part I: Available Phosphorus in Urban Runoff and
S. REPORT-DATE
December 1976 issuing date
8. PERFORMING ORGANIZATION CODE
F. Cowen^r and G. Fred Lee
ft. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Center for Environmental Studies
University of Texas at Dallas
Richardson TX 75080
10. PROGRAM ELEMENT NO.
1BA608
11. CONTRACT/GRANT NO.
Contract R-800537-02
12. SPONSORING AGENCY NAME AND ADDRESS
Environmental Research Laboratory-Duluth
Office of Research and Development
U.S. Environmental Protection Agency
Duluth MN 55804
13. TYPE OP REPORT AND PERIOD COVERED
Final ^
14. SPONSORING AGENCY CODE
EPA/600/03
ARY NOTES
Medical Bioenglneering Research ad Development Laboratory, Fort Detrick,
It. ABS
Samples of Madison urban runoff, precipitation from Madison and New York State were
analyzed for various forma of phosphorus to estimate the algal-available fraction of
each of these P forms. Urban runoff particulate P forms from Madison runoff shoved
acid extractable inorganic P in the range of 33 to 46% of the particulate P. Ranges
for the OH** and for exchange resin extractable inorganic P were 22 to 27 and 13 to
17Z of particulate P, respectively. Runoff from urban areas in the Genesee R. basin
(N.Y.) showed acid, base, and resin extractable inorganic P in the ranges of 30 to
48, 18 to 30, and 11 to 25Z of particulate P, respectively, in general agreement
with the Madison samples. Inorganic P extracted from particulate P by resin in long-
term aerobic dark incubations was similar to that extracted by the resin in short-
term tests, indicating that physical and chemical rather than microbial mineralizatio
processes were probably the key factors regulating the release of inorganic P from
the runoff particles to the solution phase.
This portion of the report includes the appendices for Part I of this study,
published as EPA-600/3-76-094a. Appendices in this section include (1) statistical
formulae and other special calculations, (b) bioassays of soluble and particulate
phosphorus with S. capricornutum, (3) dark incubations of unfiltered runoff and rivex
PU Yftfle
17.
KEY WORDS ANO DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN'ENOEO TERMS
c. COSATI I-'ield/Cfoup
precipitation (meteorology)
runoff
phosphorus
Madison Wisconsin
New York State
Lake Ontario
06F
07B
»•. DISTRIBUTION STATEMENT"
RELEASE TO PUBLIC
19. SECURITY CLASS {Tki* Rtport)
UNCLASSIFIED
20. SECURITY CLASS (Thispagt)
UNCLASSIFIED
122. PRICE
•f>C,
SPA Form 2120-1
-------
DISCLAIMER
)
Th^s repprt has been reviewed by the Environmental Research
Laboratory, U.S. Environmental, Protection Agency, and approved
for publication. Approval does not signify that the contents
necessarily reflect the views and policies of the U.S. Envir-
onmental Protection Agency, nor does mention of trade names
or commercial products constitute endorsement or recommenda-
tion for use.
11
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CONTENTS
Appendix Page
A Statistical Formulae and Other
Special Calculations 1
B Bioassays of Soluble and Particulate
Phosphorus with S. caprlcornutum. . . 4
Dark. Incubations of Unfiltered Runoff
and River Water Samples 54
Chloroform Treatment of New York
River Water 66
ill
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APPENDIX A
STATISTICAL FORMULAE AND OTHER SPECIAL CALCULATIONS
1. Arithmetic mean
n '
, x = E x.
1=1 *
n
2. Variance
2_ n- 2 f nr 2%:
s = E ,x. - ( Ex.)
i=l * i=l x
n(n-l)
3. Standard deviation
U. Coefficient of variation
cv =_s_ . 10Q%
• : X ' .'• . •' •-
5. Comparison of two mean values for significant difference
(Miller and Freund, 1965) *
x, = mean of n, observations
x2 = mean of n? observations
and x»> x. ; let x. "^2 ~^ w^tn nuH hypothesis 6=0
and one-sided alternative hypothesis 6 > 0
Compute t = x, - x-
s= „ ='
Xl X2
Where SX1 - x2 -"V Cn^D-J * Cn2
Compare t and t 05, where t 05 is taken from a table of
Miller, I., and Freund, J.E. Probability and Statistics
for Engineers, Prentice-Hall, Inc . , Englewood Cliffs,
JT7. , 432 p. (1965).
-------
t values, entered at n. + n2 - 2 degrees of freedom. If
t > t nr> the difference, 6 = x - x0 is judged significant at the
• U3 . f.
95% confidence level.
6. Coefficient of variation for PP calculated by difference (TP-TSP)
2 _
s, = variance of 3 TP replicate determinations with mean x,
2 _
s2 = variance of 3 TSP replicate determinations with mean x«
PP = x, - x0
= s_ _ = V2S1
X1~X2
SPP = s_ _ = 2S1 * 2S2
cv =-y . 100%
7. Least-squares estimators, a and b, of a and 6 in the linear
regression equation y =
-------
(Available P"|
I cone, in J = yigP/1 in 100 na Na;SOu resin leachate * DKP in flask
fcest flask J ^cent recave[y of~p f^ ^sin/xoo JuPgnate
(see Figure 6.2) CugP/1)
10. Smallest detectable available P concentration in an algal growth
bioassay
Confute C = a constant with units of cells/ml or Absorbance at 750 nm:
C s xblank * t.05 ' ^lank
where t nc is found from a table of t values, entered at n-i degrees
• uo . . .
of freedom, for a one-sided t statistic at the 95% confidence level
^ blank ^ the mean value °^ cultures grown with AAP(-P) medium,
and s.,.. is the corresponding standard deviation of these cultures).
The smallest detectable P concentration is the P concentration
corresponding to C on the standard curve of growth vs. P concen-
tration. .
11. Critical values for rejection of outliers in algal growth bioassays
(EPA, ,1971)
Rank orxier all observations: x.
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APPENDIX B
BIOASSAYS OF SOLUBLE AND PARTICULATE PHOSPHORUS
WITH S. CAPRICORNUTUM
Table No. Page
* - ^
B.I Bioassay of Runoff Particles in AAP-P
Medium, Assay Begun January 9, 1973 7
B.2 Computation of Particulate P Concen-
trations in Culture Flasks, Assay Begun
January 9, 1973 8
B.3 Bioassay,of Runoff Particles in 3X AAP-P
Medium, Assay Begun January 17, 1973 9
B.4 Bioassay of Runoff Particles in AAP-P
Medium, Assay Begun February 2, 1973 10
B.5 Bioassay of Runoff Particles in AAP-P
Medium, Assay Begun March 7, 1973 n
B.6 Bioassay of Soluble Phosphorus in Urban
Runoff, Assay Begun January 6, 1973 12
B.7 Bioassay of Soluble Phosphorus in Urban
Runoff, Assay Begun January 19, 1973 13
B.8 Bioassay of Soluble Phosphorus in Urban
Runoff, Assay Begun March 6, 1973 14
B.9 Bioassay of Soluble Phosphorus in Urban
Runoff, Assay Begun February 9, 1973 15
B.10 Bioassay of Soluble Phosphorus in Urban
Runoff, Assay Begun March.13, 1973 17
B.ll Bioassay of Particles from Madison Snow
Samples, Assay Begun April 27, 1973 18
B.12 Bioassay of Phosphorus in Unfiltered New
York Rain Gage Samples, Assay Begun
July 16, 1973 19
-------
Table No. Page
i
B.13 Bioassay of Runoff Particles in AAP-P
Medium, Assay Begun December 21, 1973 21
B.1U Bioassay of Available P in Autoclaved
New York River Waters, Assay Begun
March 29, 1973 , ' 22
B.15 Bioassay of 507-7 Particles in AAP(-P)
Medium, Assay Begun March 29, 1973 23
B.16 Bioassay of Particles from New York
River Waters and Genesee R. Basin
Samples in AAP-P Medium, Assay Begun
April l«i, 1973 24
B.17 Bioassay of Available P in Autoclaved
New York River Waters, Assay Begun
April m, 1973 26
B.18 Bioassay of Particles from New York
River and Genesee R. Basin Samples
in AAP-P Medium, Assay Begun May 8,
1973 28
B.19 Bioassay of Available P in Autoclaved
New York River Waters, Assay Begun
May 8, 1973 30
B.20 Bioassay of Particles from New York
River and Genesee R. Basin Samples in
AAP-P Medium, Assay Begun May 22, 1973 32
B.21 Bioassay of Available P in Autoclaved
New York River Water, Assay Begun
May 22, 1973 33
B.22 Bioassay of Available P in Autoclaved
New York River Waters, Assay Begun
May 31, 1973 3*
B.23 Bioassay of Particles from New York
River and Genesee R. Basin Samples in
AAP-P Medium, Assay Begun May 31, 1973 36
B.21 Bioassay of Particles from Genesee R.
Basin Samples in AAP-P Medium, Assay
Begun June 5, 1973 39
-------
Table No. Page
'B.25 Bioassay of Available P in Autoclaved
New York River Waters, Assay Begun
June 5, 1973 . 40
B..26 Bioassay of Particles from New York
River Waters and Genesee River Basin
.Samples, Assay Begun June 25, 1973 42
B.27 Bioassay of Available P in Autoclaved
New York River Waters, Assay Begun
'June 25, 1973 44
B.28 Bioassay of Particles from Genesee R.
Basin Samples in AAP-P Medium, Assay
Begun July 2, 1973 47
B.29 Available P in Autoclave^ New York
River Waters, as Calculated Using
Corrected Net A Data 49
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Table B.I. BIOASSAY OF RUNOFF PARTICLES IN AAP-P MEDIUM
Assay Begun January 9, 1973
A. ^tandarcl Curve
DRP
-------
Table B.2. COMPUTATION OF PARTICULATE P CONCENTRATIONS IN CULTURE
FLASKS . ..
Assay Begun January 9, 1973
Runoff
Sample
A- 8
mean
value
B-8
mean
value
D-8
mean
value
502-1
mean
value
Sample
TP
595
578
594
"-
589
690
705
702
699
680
712
695
696
59
63
59
60
Sample
TSPa
(ugp/l)
375
375
367
372
414
412
425
417
351
354
364
356
35
36
li
35
Mean ml sample Culture PP in
sample fi'ltered/ml dilution culture
TP-TSP AAP (-P) medium factor flasks
(UgP/1)
217 X 100/100 X 25/27 - 201
.
282 X 50/100 X 25/27 = 130
340 X 50/100 X 25/27 = 157
25 X 200/100 X 25/27 = 46
Determined on filtrates taken at the time of filtration for collecting
sample particles on membrane filters.
8
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Table B.3 BIOASSAY OF RUNOFF PARTICLES IN 3X AAP-P MEDIUM
Assay Begun January 17, 1973
A. Standard Curve3
DRP
(ygP/1)
t
0
25
50
100
200
W
B. Sample Flasks
Sample — Flask
A-9 1
2
3
4
5
6
•
B-9 1
2
3
4
5
6
F-9 1
2
3
4
5
6
(all cultures grown
Selenastrum .
cells/ml (X10 )
0
35
90
247
446
Selenastrum .
cells/ml (X10 )
132
. 112
143
131
175
152
Mean value
82
75
72
53
51
73
Mean value
123
103
88
114
96
95
Mean value
20 days)
Available P
-------
Table B.4. BIOASSAY OF RUNOFF PARTICLES IN AAP-P MEDIUM
Assay Begun February 2, 1973
A. Standard Curve
DRP
(ugP/D
0 •
25
50
100
200
B. Sample Flasks
Sample — Flask
D-ll 1
2
3
4
5
E-ll 1
2
3
4
5
•
H-ll 1
2
3
4
(all cultures grown
Selenastrum .
mm A
cells/ml (X10 )
7
64
120
248
499
Selenastrum
cells/ml (X10 )
143
1 137
124
112
88
Mean value
51
41
46
45
28
Mean value
357
345
341
370
Mean value
19 days)
Available P
(ygp/D
58
56
50
46
37_
49
28
18
21
20
11
19
143
138
137
149
142
PP in
Cultures
(ygp/i)
206
213
209
209
254
259
250
254
467
458
481
469
10
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Table B.5. BIOASSAY OF RUNOFF PARTICLES IN AAP-P MEDIUM
Assay Begun March 7, 1973
A. Standard} Curve
DRP
(ygp/D
i
0
25
50
100
200
B. Sample Flasks
Sample— rFlask
A-12 1
2
3
• • 4
5
B-12 1
2
3
4
5
D-12 1
2
3
4
5
1-12 1
2
3
4
5
(all cultures grown
Selenastrum ...
cells/ml (X10 '
8
64
126
254
516
Selenastrum ..
cells/ml (X10 4)
.215
238
203
206
162
Mean value
167
168
137
138
151
Mean value
74
85
81
71
79
Mean value
141
129
153
152
163
20 days)
Available P
(ygP/1)
84
93
79
81
63_
80
65
66
53
54
59
59
30
34
32
28
31.
31
55
50
60
59
64
PP in
Cultures
(ugP/D
189
176
173
179
186
189
192
189
118
120
125
121
359
347
361
Mean value 58 365
11
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Table B.6. BIOASSAY OF SOLUBLE PHOSPHORUS IN URBAN RUNOFF
Assay Begun January 6, 1973
A. Standard Curve
DRP
(ygP/l)
Absorbance
(750 ran)
0 0.015
25 0.079
50 0.142
100 0.217
B. Sample Flasks
Sample — Flask
A-8
B-8
D-8
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
Absorbance
(750 nm)
0,199
0.170
0.178
0.190
0.185
Mean value
0.210
0.203
0.210
0.191
0.190
Mean value
0.170
0.185
0.195
0.190
0.185
Mean value
Available P
(UgP/1)
83
64
70
77
21
73
93
86
93
77
77
85
64
74
80
77
73_
74
Chemical analyses:
DRP in TSP in
Cultures Cultures
(UgP/1) (ygP/l)
1
66 68
76 81
61 65
Five ml of filtered runoff and 20 ml (5/4X) AAP-P + one ml cells +
one ml water; cultures spiked with IX nitrate (AAP) after 6 days
12
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Table B.7. BIOASSAY OF SOLUBLE PHOSPHORUS IN URBAN RUNOFF
Assay Begun January 19, 1973
A. Standard Curve
DRP
(UgP/1
0
50
100
150
200
B. Sample Flasks*
.
Sample — Flask
A-9 1
2
3
4
5
B-9 1
2
3
4
5
F-9 1
2
3
4
5
Absorbance
(750 nm)
0.007
0.118
0.215
0.289
0.361
Absorbance
(750 nm)
0.495
0.470
0.480
0.499
0.460
0.460
0.460
0.460
0.450
0.455
0.269
0.281
0.290
0.272
0.275
Mean value
.
Chemical Analyses:
DRP in TSP in
Available P Cultures Cultures
(ygp/l) (ugP/1) (ugP/1)
All > 200 161 181
All > 200 150 167
133
141
148
135
137
139 90 102
*Five ml of filtered runoff sample + 20 ml (5/4X AAP-P) medium + one
ml water + one ml cells.
13
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Table B.8. BIOASSAY OF SOLUBLE PHOSPHORUS IN URBAN RUNOFF
Assay Begun March 6, 1973
(See assay of January 19)
A. Standard Curve
DRP
(ygp/D
0
100
200
300
400
Absorbance
(750 nm)
0.017
0.166
0.328
0.432
0.509
B. Sample Flasks
Absorbance Available P
Sample—Flask ' (750 nm) (ygP/1)
Chemical analysis:
TSP in
Culture Flask
(ygP/1)
B-9
B-9 +
50
ygp/i
B-9 +
100
y gp/i •
•
i
2
3
4
5
1
2
3
4
5
1
2
3
4
5
0.455
0.465
0.475
0.470
0.465
mean value
0.500
0.530
0.522
0.525
0.510
mean value
0.525
0.535
0.535
0.540
0.548
327
342
355
348
342
343
391
>400
>400
>400
>400
> 398
>400
>400
> 400
>400
>400
,
167
217
267
Five ml of same sample as used in Jan. 19, 1973 assay, + 20 ml
(5/4X) AAP-P medium + one ml cells + one ml water + one ml water
or DRP (27X) spike
14
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Table B.9. BIOASSAY OF SOLUBLE PHOSPHORUS IN URBAN RUNOFF
Assay Begun February 9, 1973
A. Standard Curve
i .
DRP Absorbance
(ygP/1)
0
25
50
100
200
B. Sample Flasks*
i .
(750 ran)
0.010
0.070
0.116
0.206
0.365
DRP in TSP in
Absorbance Available P Cultures Cultures
Sample — Flask
D-10 1
2
3
4
5
•
E-10 1
2
3
4
5
'
H-10 1
2
3
4
5
D-ll 1
2
3
4
5
(750 ran)
0.127
' 0.125
0.121
0.120
0.125
mean
0.020
0.023
0.025
0.025
0.025
mean
0 113
0.091
0.101
0.100
0.103
mean
0.090
0.111
0.105
0.103
0.105
(ygp/i) (yqp/i) (ygP/1)
59 45 50
58
56
56
58
value 57
4 24 30
5
5
5
^••••b
value 5
52 81 98
41
46
45
47
value 46
40 47 48
50
48
47
48
mean value
47
IS
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Table B.9. (continued)
Sample — 'Flask
E-ll
•
1
2
3
4
5
Absorbance
(750 nm)
0.034
0.023
0.029
0.030
0.022
Available P
(ygP/D
10
- 4
8
8
4
DRP in
Cultures
(ygP/1)
22
TSP in
Cultures
(MgP/1)
25
H-ll
1
2
3
4
5
mean value 7
0.040 13
0.041 14
0.032 8
0.045 16
0.035 11
M^B^HI
mean value 12
20
21
Samples D-10, D-ll, H-ll: five ml filtered runoff + 20 ml (5/4X)
AAP(-P) medium + one ml cells + one ml of water
Samples E-10, H-10, E-ll: 20 ml filtered runoff + five ml (5X)
AAP(-P) medium + one ml cells + one ml water
16
-------
•
Table B.10. BXOASSAY OP SOLUBLE PHOSPHORUS IN URBAN RUNOFF
Assay Begun March 13, 1973
A. Standard Curve
DRP
(uerP/D
,0
25
50
100
Absorbance
(750 ran)
0.010
0.042
0.081
0.141
B. Sample Flasks*
Sample — flask*
A-12
B-12
D-12
•
1-12
1
2
3
4
5
1
2
3
4
1
2
3
4
5
1
2
3
4
5
Absorbance Available P
(750 nm) (VgP/1)
0.121
0.099
0.135
0.122
0.122
mean value
0.165
0.160
0.161
0.160
Mean value
0.110
0.110
0.096
0.108
0.092
mean value
0.029
0.028
0.025
0.028
0.025
mean value
85
68
95
85
85
84
120
115
116
115
116
76
76
65
74
62
71
14
13
11
13
11
12
DRP in TSP in
Cultures Cultures
(UqP/1) (UcjP/1)
49 51
•
64 65
46 48
22 34
•
Samples A-12, B-12, D-12: five ml filtered runoff + 20 ml (5/4X) AAP(-P)
medium + one ml cells + one ml water. Sample 1-12: 20 ml filtered runoff
+ five ml (5X) AAP(-P) medium + one ml cells + one ml water
17
-------
Table B.ll. BIOASSAY OF PARTICLES FROM MADISON SNOW SAMPLES
i
Assay Begun April 27, 1973
A. Standard Curve
DRP
(ygp/D
0
10
25
50
100
200
B. Sample Flasks
Sample — Flask
Snow 1
Picnic Pt. 2
3
4
5
•
Snow 1
Roof of 2
City-County 3
Bldg. 4
5
Snqw 1
Near Meat 2
Packing 3
Plant 4
Selenastrum .
cells/ml (X10 )
9
26
62
167
305
647
Selenastrum
cells/ml (X10 )
48
29
33
31
41
Mean value
1
0
1
2
2
Mean value
86
68
64
77
Mean value
Available P
(ygp/D
20
11
12
12
17
14
< 2
< 2
< 2
< 2
< 2
< 2
35
27
26
31
30
PP in Cultures
(ygp/D
82
83
82
118
118
118
135
127
131
18
-------
Table B.12.
BIOASSAY OF PHOSPHORDS IN UNFILTERED NEW YORK
RAIN GAGE SAMPLES
Assay Begun July 16, 1973
A. Standard Curve
DRP Selenastrum .
(UgP/1) cells/ml (X10 )
0
10
25
50
B. Sample Flasks
Sample—Flask
May 601 1
2
3
4
5
May 602 1
2
3
4
5
May 606 1
2
3
4
5
June 601 1
2
3
4
5
10
38
86
154
Selenastrum .
MHMH^MMMMMMMVMM ^A
cells/ml (X10 )
111
88
102
93
102
mean value
22
19
18
18
17
mean value
6
5
6
6
4
mean value
78
98
96
98
103
mean value
Available P
(yqP/1)
35
27
32
29
32
31
4
3
3
3
•^••••B
3
< 2
< 2
< 2
< 2
< 2
< 2
24
31
30
31
32
31
TP in Cultures
(VigP/1)
307
315
312
311
62
64
65
64
327
330
319
325
53
53
53
53
19
-------
Table B.12. (continued)
Sample — Flask
June 603
June 604
1
2
3
4
5
1
2
3
4
5
Selenastrura
cells/ml (X10 )
14
19
18
22
19
mean value
117
172
137
114
124
mean value
Available P
(VigP/1)
2
3
3
4
3
3
37
56
44
36
40
43
TP in Cultures
(ygp/D
77
74
84
78
47
49
47
48
The following samples were quantitated by comparison of their Absorbance
at 750 nm (A__Q), compared to the mean A of the 10 ygP/1 standard =
0.035:
Sample
May 603
May 604
May 605
June 602
June 605
June 606
June 608
Average
A750
0.009
0.011
0.025
0.011
0.012
0.012
0.017
Approximate Available P
(ugp/i)
< 10
< 10
< 10
< 10
< 10
< 10
< 10
Average
TP in Cultures
(ygp/D
3
4
259
7
7
9
256
20
-------
Table B.13. BXOASSAY OF RUNOFF PARTICLES IN AAP-P MEDIUM
, : Assay Begun December 21, 1973
A. Standard Curve*
DRP Selenastrum ..
(VgP/1) cells/ml (X10 )
0
25
50 ,
100
B. Sample Flasks
Sample — Flask
502-1 1
2
3
4
e
6
< 1
65
118
193
Selenastrum .
cells/ml (X10 )
29
10
12
24
38
36
mean value
Available P
(ycrP/1)
10
3
4
8
14
13
9 ' ' •
PP in
Cultures
(UaP/1)
40
44
44
43
*25 ml AAP-P •(• one ml cells + one ml DRP (27X) spike (27 ml volume)
values are averages of six flasks
Particles from sample 502-1 in 25 ml AAP-P medium + one ml cells
(26 ml volume)
21
-------
Table B.14.
BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WATERS
Assay Begun March 29, 1973
A. Standard Curve
DRP Absorbance
(ygp/D
0
25
50
100
200
(750 nm)
0.010
0.050
0.090
0.180
0.339
•
•
B. Sample Flasks
Sample — Flask
No. 27 1
2
3
4
5
No. 28 1
2
3
4
5
No. 29 1
2
3
4
5
Absorbance
(750 nm)
0.010
0.010
0.010
0.010
0.010
mean value
0.119
0.115
0.110
0.121
0.111
mean value
0.165
0.149
0.155
0.160
0.178
Available P TSP in Cultures
(ygP/1) (ygP/l)
< 1
< 1
< 1
< 1 4
< 1 5
< 1 4
68
64
60
68 47
60 47
64 47
95
85
88
91 56
102 56
mean value
92
56
22
-------
Table B.15. BIOASSAY OF 507-7 PARTICLES IN AAP (-P) MEDIUM
Assay Begun March 29, 1973
Sanple—Flask
507-7
1
2
3
4
5
Selenastrum* .
Cells/ml (X10 )
0
0
0
0
0
mean value
Available P
(UsP/1)
0
0
0
0
0
0
PP in Cultures
(UgP/1)
62
62
58
61
* No cell counts were made for standards since the growth in the
sanples was negligible.
23
-------
Table B.16.
BIOASSAY OF PARTICLES FROM NEW YORK
RIVER WATERS AND GENESEE R. BASIN
SAMPLES IN AAP-P MEDIUM
Assay Begun April 14, 1973
A. Standard Curve
DRP
(ugp/i)
0
25
50
100
200
Selenastrum .
cells/ml (X10 )
1
1
64
107
227
572
B. Sample Flasks
Sample — Flask
No. 34
No.
502-7
•
1
2
3
4
S
1
2
3
4
5
Selenastrum
cells/ml (X10 )
8
6
9
11
6
mean value
76
70
79
56
53
mean value
Available P
(ugP/1)
3
2
4
5
2
3
33
31
35
25
24
30
PP in Cultures
(ugP/1)
124
123
116
108
118
126
119
142
140
128
137
No.
502-8
1
2
3
4
5
30
43
56
53
52
13
19
25
23
23
99
99
103
mean value
21
100
-------
Table B.16. (continued)
Selenastrum Available P PP in Cultures
Sample—Flask cells/ml (X10 ) (UqP/1) (UgP/1)
No. 1 ' 9 4
504-8 2 94
3 9 4 57
4 12 5 55
5 7 3 60
mean value 4 57
i
No. 1 4 1
507-8 2 7 3
3 . 10 4 68
4 6 2 65
5 5 2 59
mean value 2 64
25
-------
Table B.17.
BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WATERS
Assay Begun April 14, 1973
A. Standard Curve
DRP Absorbance
(ygP/1)
0
25
50
100
200
B. Sample Flasks
Sample — Flask
No. 31 1
2
3
4
i
No. 32 1
2
3
4
5
No. 33 1
2
3
4
5
(750 nm)
0.000
0.052
0.071
0.131
0.228
Absorbance
(750 nm)
0.055
0.058
0.053
0.055
mean value
0.004
0.004
0.004
0.004
0.004
mean value
0.010
0.010
0.012
0.011
0.010
mean value
Available P
(ygp/D
40
42
38
40
40
1
1
1
1
1
1
5
5
7
5
5
5
TSP in Cultures
(pgP/1)
30
28
31
30
6
6
6
10
10
10
26
-------
Table B.17 (continued)
Sample—Flask
No. 34
No. 35
No. 36
1
2
3
4
5
1
2
3
•4
5
1
2
3
4
5
Absorbance
(750 nm)
0.047
0.046
0.042
0.047
0.045
nean value
0.125
0.113
0.120
0.122
0.129
nean value
0.013
0.010
0.019
0.018
0.019
•can value
Available P
(U9P/1)
32
32
30
32
31
31
95
85
90
92
100
92
7
5
12
11
12
9
TSP in Cultures
(UcrP/1)
30
30
29
30
53
52
53
53
14
16
14
15
27
-------
Table B.18.
BIOASSAY OF PARTICLES FROM NEW YORK RIVER AND
GENESEE R. BASIN SAMPLES IN AAP-P MEDIUM
Assay Begun May 8, 1973
A. Standard Curve
DRP
(ygp/D
0
25
50
100
200
B. Sample Flasks
Selenastrum
cells/ml (X10 )
10
77
157
323
625
Selenastrum „
Sample—Flask cells/ml (X10 *)
No. 42 1
2
3
4
5
i
No. 43 1
2
3
4
5
No. 44 1
, 2
3
4
•
56
83
34
9
1
mean value
.4
5
0
0
0
mean value
17
22
18
23
mean value
-
Available P
(ugp/l>
18
27
10
< 2
< 2
< 12
< 2
< 2
< 2
< 2
< 2
< 2
3
5
3
5
4
PP in Cultures
(ygp/D
187
190
185
187
306
308
309
137
128
133
133
28
-------
Table B.18 (continued)
Selenastrum . Available P PP in Cultures
Sample—Flask cells/ml (Xio" ) (ygP/1) (yqP/1)
No.
502-10
1
2
3
4
5, •
236
282
372
93
299
mean value
73
87
114
29
92
79
333
320
331
328
No. 1 5 < 3
507-12 2 0 < 3
3 1 < 3 207
4 0 < 3 204
• ! 5 0 < 3 218
mean value < 3 210
29
-------
Table B.19. BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WAfERS
Assay Begun May 8, 1973
A. Standard Curve
DRP
(ygp/D
0 '
25
50
100
200
Absorbance
(750 nm)
0.010
0.046
0.092
0.183
0.281
-
B. Sample Flasks
Sample — Flask
No. 40
,
No. 41
No. 42
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
Absorbance
(750 nm)
0.010
0.010
0.010
0.011
0.010
mean value
0.010
0.010
0.010
0.010
0.010
mean value
0.060
0.055
0.060
0.062
0.055
mean value
Available P
(ygP/1)
< 1
< 1
< 1
< 1
< 1
< 1
< 1
< 1
< 1
< 1
< 1
< 1
31
28
31
32
28
30
TSP in Cultures
(ygP/1)
5
3
4
5
4
4
32
32
32
30
-------
Table B.19. (continued)
Sample—Flask
Mo. 43 1
2
3
4
5
Absorbance Available P TSP in Cultures
(750 nin) (UgP/1) (UqP/1)
0.079
0.079
0.087
0.078
0.060
mean value
42
42
48
42
32
41
39
39
39
31
-------
Table B.20.
BIOASSAY OF PARTICLES FROM NEW YORK RIVER AND
GENESEE R. BASIN SAMPLES IN AAP-P MEDIUM
Assay Begun May 22, 1973
A. Standard Curve
DRP
(ugP/D
0
10
25
100
Selenastrum
cells/ml
5
29
87
347
(X10 *)
B. Sample Flasks
Sample — Flask
No. 47 1
2
3
4
5
Selenastrum •
cells/ml (X10 )
' 25
16
14
21
4
Available P
(ygp/D
8
5
4
6
< 2
PP in Cultures
(ygP/1)
239
230
238
mean value
< 5
236
No.
502-11
1
2
3
4
2
2
2
1
mean value
< 2
< 2
< 2
< 2
Mt^^H
< 2
164
164
164
164
^^WH^V^*
164
No.
507-11
1
2
3
4
5
5
4
3
5
5
< 2
< 2
< 2
< 2
< 2
70
68
66
mean value
< 2
68
-------
Table B.21.
BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WATERS
Assay Begun May 22, 1973
A. 'standard Curve
DRP Absorbance
(ygP/1) (750 nm)
0
10
25
50
100
, 200
300
400
0.009
0.031
0.075
0.09B
0.209
0.308
0.395
0.423
Selenastrum .
cells/ml (X10 )
5
29
87
341
B. Sample Flasks
Sample — Flask
No. 47 1
2
3
4
5
Absorbance
(750 nm)
Available P TSP in Cultures
(ugP/1) (ugP/1)
0.125 61
0.125 61
0.128 63 56
0.122 60 53
0.130 67 51
mean value 61 53
C. Comparison: quantitation by cell counting
Sample— Flask
No. 47 1
2
'3
4
5
Selenastrum .
cells/ml (X10 )
140
141
138
139
147
mean value
Available P
(UgP/D
40
41
40
40
42
41
TSP in Cultures
(ygp/D
53
33
-------
Table B.22.
BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WATERS
Assay Begun May 31, 1973
A. Standard Curve
(A) (S)
DRP Absorbance Selenastrum A
(ugp/i)
0
10
25
50
100
200
300
(750 nm) cellS/ml
0.008 6
0.026 28
0.053 74
0.097 163
0.186 323
0.301 742
0.363 1002
(X10 M)
DRP
(ygp/l) (A)
400 0.404
500 0.437
600 0.459
(S)
1169
1241
1365
B. Sample Flasks
Sample — Flask
No. 49 1
2
3
4
5
No. 50 1
2
3
4
' 5
No. 51 1
2
3
4
5
Absorbance
(750 nm)
0.011
0.011
0.010
0.010
0.010
mean value
0.010
0.010
0.010
0.011
0.010
mean value
0.229
0.242
0.261
6.219
0.274
mean value
Available
(ugP/1)
< 3
< 3
< 3
< 3
< 3
< 3
< 3
< 3
< 3
< 3
< 3
< 3
126
137
150
120
163
139
P TSP in Cultures
(ygp/l)
12
13
11
12
13
10
12
12
84
85
82
83
34
-------
Table B.22. (continued)
Sample — Flask
No. 52 1
2
3
4
NO. 53 1
2
3
4
5
Absorbance
(750 nm)
0.130
0.137
0.132
0.133
mean value
0.031
0.045
0.039
0.040
0.041
mean value
•
C. Comparison: quantitation by cell
Sample—Flask
No. 51 1
2
3
4
NO. 52 1
2
3
4
Selenastrum .
cells/ml (X10 )
300
302
296
310
mean value
151
156
150
154
mean value
Available P
(UCfP/1)
68
71
69
69
69
12
21
16
17
J.8
17
counting
Available P
(ygp/D
92
92
90
A4_
92
50
51
50
51
50
TSP in Cultures
-------
Table B.23.
BIOASSAY OF PARTICLES FROM NEW YORK
RIVER AND GENESEE R. BASIN SAMPLES
IN AAP-P MEDIUM
Assay Begun May 31, 1973
A. Standard Curve
DRP
(ugp/i)
0
10
25
50
100
200
300
Selenastrum .
cells/ml (X10 )
6
28
74
163
323
742
1002
DRP
(ygp/D
400
500
600
Selenastrum
cells/ml (X10 )
1169
1241
1365
B. Sample Flasks
Sample—Flask
No. 50 1
2
3
4
5
Selenastrum
cells/ml (X10 )
15
23
1
18
11
mean value
Available P
(jagP/1)
4
7
< 3
5
< 3
< 4
PP in Cultures
(ugp/i)
79
78
79
79
No. 50 1
(auto- 2
claved) 3
4
5
No. 50 + 1
50 ygP/1 2
(auto- 3
claved) 4
138
131
138
122
156
mean value
287
251
266
278
46
43
46
41
51
PMH^BWA"
45
88
78
82
85
79
mean value
83
-------
Table B.23. (continued)
Sample—Flask
Selenastrum
cells/ml (X10
-4,
Available P
(UqP/1)
PP in Cultures
(ygP/1)
No. 51
Mo. 51
1
2
3
4
1
2
3
4
5
No. 5,1+1
50 ygP/1 2
(auto- 3
claved)
No. 52
No. 52
(auto-
claved)
No. 53
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
9
11
2
1
mean value
159
155
136
131
132
mean value
*299
307
279
mean value
4
2
2
0
0
mean value
169
204
217
202
160
mean value
0
4
0
1
1
mean value
< 3
52
51
45
43
44
••••^•^
47
91
93
85
•••MHM^M
90
< 3
55
65
69
65
55
62
117
116
112
MtMBB^M
us
115
141
< 3
141
65
69
65
••^•M
66
37
-------
Table B. 23 (continued)
Selenastrum . Available P PP in Cultures
Sample— Flask cells/ml (X10 ) _ (ygP/1) _ (ygP/1)
No. 53 1 71 24
(auto- 2 92 31
claved) 3 , 107 36
4 61 20
5 HO 37
mean value 30 66
38
-------
Table B.24.
BIOASSAY OF PARTICLES FROM GENESEE R.
BASIN SAMPLES IN AAP-P MEDIUM
Assay Begun June 5, 1973 •
A. Standard Curve
DRP
(UcrP/1)
0
10
25
50
100
200
300
Selenastrum .
•««B«I^^M«^BMIW«M *•«
cells/ml (X10 ]
5
29
73
173
329
639
994
I
B. Sample Flasks
Sample— Flask
No. 501-12* 1
2
3
4
5
NO. 502-12 1
2
3
4
5
Selenastrum
cells/ml (X10~ )
19
4
12
16
15
mean value
4
32
19
35
19
mean value
Available P
(ygp/i)
5
< 2
3
4
4__
< 4
< 2
9
5
10
^^^•^^
< 6
PP in Cultures
(W
147
136
155
146
79
78
85
81
^Inoculated on June 6, 1967, with same cell suspension used on
standards and sample No.502-12 on June 5, 1973.
39
-------
Table B.25.
BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WATERS
Assay Begun June 5, 1973
A. Standard Curve
DRP
(ygp/D
0
10
25
50
100
200
300
Absorbance
(750 nm)
0.010
0.027
0.056
0.098
0.202
0.302
0.381
Selenastrum
cells/ml (X10
5
29
73
173
329
639
994
-4
B. Sample Flasks
Sample — Flask
No. 52a 1
2
3
4
5
No. 52+1
25 ugP/1 2
3
4
5
No. 52+1
50 ygP/1 2
3
4
5
Absorbance Available P
(750 nm) (ugP/1)
0.152
0.150
0.149
0.145
0.159
mean value
0.230
0.205
0.238
0.235
0.227
mean value
0.280
0.290
0.280
0.240
0.255
mean value
77
76
75
73
81
76
125
130
131
129
123
127
171
182
171
132
147
161
TSP in Cultures
(ugP/1)
52
58
53
54
79
104
a
20 ml of sample No. 52 + one ml cells + one ml of water or 27X ortho-
phosphate spike; the sample No. 52 used in these tests was the same
as used in the bioassay begun May 31, 1973.
40
-------
Table B.25 (continued)
Sample — Flask
No. 52 + 1
100 ygP/1 2
, 3
4
5
No. 52 -f 1
200 WgP/1 , 2
3
4
5
Absorbance
(750 nm)
6.301
0.302
0.329
0.342
0.320
mean value
0.362
0.321
0.339
0.345
0.340
mean value
Available P
(ygP/1)
196
- 197
230
248
220
218
277
221
243
252
245
248
TSP in Cultures
(ygP/1)
54
254
C. Comparison: quantitation by counting cells
Selenastrum .
Sample—Flask cells/ml (X10~ )
Available P
(ygP/1)
TSP in Cultures
(ygP/1?
No. 52
1
2
3
4
5
158
151
171
159
169
mean value
48
46
53
49
52
MB^«M*
50
54
41
-------
Table B.26.
BIOASSAY OF PARTICLES FROM NEW YORK RIVER
WATERS AND GENESEE RIVER BASIN SAMPLES
Assay Begun June 25, 1973
A. Standard Curve
PRP
(UgP/1)
0
10
25
50
100
200
B. Sample Flasks
Selenastrum
cells/ml (X10 )
2
20
78
172
374
746
Selenastrum „ Available P
Sample — Flask
No. 56 1
• (auto- 2
claved) 3
4
5
No. 58 1
(not 2
auto- 3
claved) 4
5
No. 58 1
(auto- 2
claved) 3
4
5
cells/ml (X10 )
86
97
93
91
108
mean value
competing algae
present; could
not count £.
capricornutum
accurately
252
298
385
380
235
(ygp/D
27
30
29
28
33
29
70
82
105
103
66
PP in Cultures
(UgP/1)
87
84
90
87
231
246
234
mean value
85
237
42
-------
Table B.26 (continued)
Selenastrum , Available P
Sample — Flask
No. 59
(auto-
claved)
i
No. 60
(auto-
c laved)
No. 501-13
(auto-
claved)
•
-
No. 507-13
(auto-
c laved)
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
cells/ml (X10 )
203
183
191
158
170
mean value
160
156
155
198
178
mean value
46
48
49
49
50
mean value
45
56
51
51
42
mean value
(ugP/D
58
52
55
46
49
52
46
45
45
57
51
49
16
17
17
17
17
17
16
20
18
18
15
17
PP in Cultures
(ygp/i)
163
163
162
163
196
190
182
189
99
106
103
103
221
225
225
224
43
-------
Table B.27. BIOASSAY OF AVAILABLE P IN AUTOCLAVED
NEW YORK RIVER WATERS
Assay Begun June 25, 1973
1
A. Standard Curve
' DRP
(ugp/l)
0
10
25
50
100
200
300
Absorbance
(750 run)
0.004
0.025
0.067
0.102
0.225
0.318
0.404
Selenastrum
cells/ml (X10 )
2
20
78
172
374
746
-
B. Sample Flasks
Sample — Flask
No. 56 1
2
3
4
No. 56 -f 1
25 VgP/1 2
3
4
5
No. 57 1
2
3
4
5
No. 57 + 1
25 UgP/1 2
3
4
5
Absorbance
(750 ran)
0.019
0.018
0.019
0.020
mean value
0.100
0.111
0.109
0.105
0.112
mean value
0.011
0.010
0.015
0.011
0.010
mean value
0.102
0.102
0.101
0.102
0.105
mean value
Available P TSP
(ygp/D
7
7
7
7
7
43
48
46
45
48
46
3
3
5
3
3
3
43
43
42
43
45
43
in Cultures
(ygP/1)
13
13
13
38
11
11
11
36
44
-------
Table B.27. (continued)
1
Sample — Flask
No. 58
I
No. 58 +
25 ygP/1
No. 59
No. 59 +
25 pgP/1
No. 59
rtx)a
No. 59
(>»X)a +
25 ugp/l
1
2
3
4
5
1
2
3
4
5
1
2
3
4
1
2
3
4
5
1
2
3
4
1
2
3
4
.5
Absorbance
(750 nm)
0.140
0.139
0.138
0.142
0.140
mean value
0.228
0.211
0.225
0.235
0.230
mean value
0.152
0.160
0.158
0.162
mean value
0.241
0.245
0.240
0.245
0.240
mean value
0.080
0.079
0.080
0.078
mean value
0.180
0.187
0.183
0.185
0.188
mean value
Available P
(ygp/D
60
60
60
62
60
60
101
93
100
106
102
100
67
70
69
72
70
110
112
110
112
110
111
33
33
33
32
33
80
82
81
82
83
82
TSP in Cultures
(ugp/l)
50
50
50
75
57
55
56
81
28
53
*Ten ml of sample No. 59 diluted to 20 ml with glass dist. water
before assay.
45
-------
Table B.27. (continued)
1 '
Sample — Flask
No.
No.
25
C.
60 1
2
3
4
60 + 3.
ygP/1 '2
3
4
5
Comparison :
Sample — Flask
No.
No.
25
56 1
2
3
4
5
56 + 1
ygP/1 2
3
4
5
Absorbance
(750 nm)
0.065
0.070
0.065
0.069
mean value
0.169
0.170
0.170
0.166
0.173
mean value
quantitation by
Selenastrum
cells/ml (X10
13
12
15
15
9
mean value
101
114
97
115
106
mean value
Available P TSP in Cultures
(ygp/D (ygP/D
27
29
27 38
28 38
28 38
75
75
75
73
77
75 63
counting cells
TSP in
Available P Cultures
) (ygP/1) (UgP/1)
7
7
8
8
5
7 13
31
34
30
34
32
32 38
Recovery of 25 VgP/1 spike = 100%
46
-------
Table 3.28.
BIOASSAY OF PARTICLES FROM GENESEE R. BASIN
SAMPLES IN AAP-P MEDIUM
Assay Begun July 2, 1973
A. Standard Curve
DRP
(ygp/i)
0
10
25
50
100
B. Sample Flasks
Sample — Flask
No. 501-14 1
2
3
4
5
No. 501-14 1
(auto- 2
claved) 3
4
5
No. 502-14 1
2
,".••• 3
4
5
No. 502-14 1
(auto- 2
claved) 3
4
5
Selenastrum .
cells/ml (X10 *)
7
23
94
162
336
Selenastrum
cells/ml (X10-4)
7
17
15
12
23
mean value
38
34
39
35
33
mean value
13
1
0
0
1
mean value
138
152
160
146
143
mean value
Available P
(ygP/1)
< 2
6
5
4
8
< 5
12
11
12
11
10
11
4
< 2
< 2
< 2
< 2
< 2
42
45
48
44
43
44
PP in Cultures
-------
Table B.28. (continued)
Selenastrum Available P PP in Cultures
Sample—Flask cells/ml (XIQ" ) (ugP/1) (ygp/l)
Ncj. 507-14 1 38 12
2 30 10
3 45 14 440
4 28 ,9 458
5 44 14 434
mean value 12 444
No. 507-14 1 162 48
(auto- 2 179 54
claved) 3 167 50
4 164 49
5 169 51
mean value 50 481
48
-------
Table B.29.
AVAILABLE P IN AUTOCLAVED NEW YORK RIVER
WATERS AS CALCULATED USING CORRECTED
NET A DATA
Sample
No.
27
33
41
50
56
32
40
49
57
34
42
51
58
28
29
31
35
43
47 .
52e
52e
59
5900*
Net Mean Corrected Corrected Net Available
A. . Net Mean Mean Blank Mean AVSQ •*• In Bioassay
Bioassay A750b A750 Mean Blank A?50 Flasks
0.000
o'.oii
0.000
0.002
0.015
0.004
0.000
0.002
0.007
0.045
0.048
0.237
0.136
0.105
0.151
0.055
0.122
0.067
0.117
0.125
0.141
0.154
0.075
Niagara
0.000
0.008
0.000
0.001
0.010
Niagara
0.003
0.000
0.001
0.005
0.031
0.034
0.166
0.095
0.073
0.106
0.039
0.085
0.047
0.082
0.087
0.099
0.108
0.052
,,
(ugP/i)
R. at Ft. Niagara
0.010
0.000
0.010
0.008
0.004
R. at Beaver
0.000
0.010
0.008
0.004
Genesee R.
0.000
0.010
0.008
0.004
Oswego R.
0.019
0.010
0.000
0.000
0.010
0.009
0.008
0.010
0.004
0.004
0.010
0.008
0.010
0.009
0.014
I. Park
0.003
0.010
0.009
0.009
0.031
0.044
0.174
0.099
0.083
0.116
0.039
0.085
0.057
0.091
0.095
0.109
0.112
0.056
< 1
4
< 1
< 3
5
< 1
< 1
< 3
3
22
23
92
42
45
65
27
65
29
42
47
53
48
24
Mean A_ n of sample cultures minus the mean A__.of. the
blank culti 75°
:ures
}Net mean *750 divided by 1.43
'Found by comparing the corrected net mean A750 to a
standard curve of A__ vs. P concentration tor standard
AAP cultures
Assayed May 31, 1973
^Assayed June 5, 1973
49
-------
APPENDIX C
DARK INCUBATIONS OF UNFILTERED RUNOFF AND
RIVER WATER SAMPLES
Table No. Page
C.I Dark Incubation Begun September 26, 1972 53
C.2 Dark Incubation Begun November 9, 1972 55
Unfiltered Runoff Samples
C.3 Dark Incubation Begun January 8, 1973 56
Unfiltered Runoff Samples
C.4 Dark Incubation Begun January 24,1973 57
Unfiltered Runoff Samples
C.5 Dark Incubation Begun February 7, 1973 58
Unfiltered Runoff Samples
C.6 Dark Incubation Begun March 8, 1973 59
Unfiltered Runoff Samples
C.7 Dark Incubation Begun December'1, 1972 60
C.8 Dark Incubation Begun April 13, 1973 61
Unfiltered River Water Samples
C.9 Dark Incubation Begun May 7, 1973 63
Unfiltered River Water Samples
C.10 Dark Incubation Begun May 31,1973 64
Unfiltered River Water Samples
C.ll Dark Incubation Begun June 8, 1973 65
Unfiltered River Water Samples
C.I2 Dark Incubation Begun June 22,1973 66
Unfiltered River Water Samples
50
-------
APPENDIX C
DARK INCUBATIONS OF UNFXLTBRED
RUNOFF AND RIVER WATER SAMPLES
Table C.I. Dark Incubation Begun September 26, 1972
A. Initial Period of Storage at 4°
Sample No.
23-Oswego R.
24-Oswego R. Mouth
25-Black R.
26-Osvego R.
B-4 Madison Runoff
Initial
DRPa
(VgP/1)
49
41
14
79
240
C
Storage
Period
(days)
35
21
21
21
7
Determined prior to storage
B. Dark Incubation at 17-23°C (Begun Sept. 26, 1973)
Sample
No.
23
mean
values
24
nean
values
i
25
mean
values
DRP
(IKJP/I) after
Sample
23 days
61
69
65
61
58
^^MM
60
25
21
23
50 days
73
21
72
62
59
61
31
27_
29
incubation
Sample +
23 days
65
__
—
64
__
—
20
— ..
—
Resin
50 days
78
21
76
53
59
^MH«B
56
22
30
26
51
-------
Table C.I. (continued)
26 112 120 95 114
110 109 — 103
mean ——
values 111 114 ~ 108
B-4 274 244 321 322
248 240 — 315
mean
values 261 242 — 318
All samples except B-4 were filtered through glass fiber
filters for DRP determined during the incubations; B-4
was filtered through 0.45 micron pore size membrane filters.
-------
Table C.2. DARK INCUBATION BEGUN NOVEMBER 9, 1972
•Unfiltered Runoff Samples
Runoff
Sample
B-7
P-7
402-8
407-8
Initial
DRP Sample Only
(ygP/1) 25 Days
576 292
293
255
mean value 280
300 140
150
155
mean value 148
70 45
43
ii
mean value 44
27 31
32
28
mean value 30
DRP (ygP/1) after incubation
Sample +
25 days
564
569
595
576
391
396
391
393
107
106
111
108
92
91
92
92
Resin
50 days
641
650
652
648
407
393
386
395
106
113
108
109
107
104
102
104
-------
Table C.3. DARK INCUBATION BEGUN JANUARY 8, 1973
Unfiltered Runoff Samples
Runoff
Sample
A-8
B-8
D-8
Initial DRP (ygP/1)
DRP Sample Only
(ugP/1) 25 days
354 347
348
365
mean value 353
410 410
422
425
mean value 419
328 380
364
381
mean value 375
50 Days
542
552
557
550
467
482
476
475
385
407
395
396
after incubation
Sample
25 Days
372
389
401
387
455
476
470
467
381
399
391
390
+ Resin
50 Days
430
391
409
410
\
458
464
472
465
434
419
414
422
-------
Table C.4. DARK INCUBATION BEGUN JANUARY 24, 1973
Unfiltered Runoff Samples
Initial. . DRP (ygP/1) after incubation
Runoff DRP Sample Only Sample + Resin
Sample (yg'P/1) 25 Days 50 Days 25 Days 50 Days
A-9 868 942 947 930 983
952 984 957 967
925 1020 942 961
mean value 940 984 943 970
Ol
01 B-9 812 867 868 880 853
846 900 875 869
865 902 893 906
mean value 859 890 883 876
P-9
D-10
486 545
530
537
mean value 537
244 320
300
304
mean value 208
520
530
534
528
287
285
280
284
545
548
546
546
330
331
328
330
562
545
561
556
342
343
341
342
-------
tn
0\
Table C.5. DARK INCUBATION BEGUN FEBRUARY 7, 1973
Unfiltered Runoff Samples
Initial DRP (pgP/1)
Runoff
Sample
D-ll
E-ll
H-ll
DRP Sample
(ygP/1) 13 Days
252 233
233
238
mean value 235
30 10
12
13_
mean value 12
110 41
44
ii
mean value 44
Only
26 Days
256
264
268
263
15
14
13^
14
55
54
53_
54
after incubation
Sample
13 Days
286
288
276
284
35
39
36
36
106
100
102
103
+ Resin
26 Days
315
329
304
316
s
32
32
32
32
103
108
103
105
-------
Table C.6. DARK INCUBATION BEGUN MARCH 8, 1973
Unfiltered Runoff Samples
in
Runoff
Sample
A-12
B-12
D-12
1-12
Initial
DRP
(ugP/1)
264
mean value
347
mean value
247
mean value
30
-
mean value
DRP (yqP/1)
Sample Only
13 Days 21 Days
295
290
299
295
345
354
349
349
194
197
198
196
35
33
36
35
293
305
309
302
394
406
397
399
249
257
250
252
84
84
82
83
after incubation
Sample
13 Days
299
306
302
337
337
— -
337
287
278
307
291
84
114
107
102
+ Resin
23 Days
309
313
311
311
385
395
397
392
328
340
340
336
153
132
141
142
-------
Table C.7. DARK INCUBATION BEGUN DECEMBER 1, 1972
Runoff
Sample
402-9
409-0
404-8
409-8
Initial DRP (ygP/1) after
DRP Sample Only (No
(ygP/1) 25 Days
55 67
70
69
71
70_
mean value 69
13 30
27
29
28
_2_9
mean value 29
175 205
212
216
214
^20?
mean value 211
< 1 12
11
13
11
12
incubation
Resin)
50 Days
64
66
70
70
68
33
32
32
33
31
32
214
225
214
215
217
15
18
18
14
12
mean value 12 15
58
-------
Table C.8. DARK INCUBATION BEGUN APRIL 13, 1973
Unfiltered River Water Samples
In
Initial
DRP (UgP/1) after incubation
DRP Sample Only
Sample
No. 31
No. 32
No. 33
No. 34
(UgP/1) 14 Days
43 46
47
47
mean value 47
2 6
6
6
mean value 6
5 11
12
14
mean value 12
26 30
32
32
mean value 31
27 Days
35
34
30
33
0
2
0_
1
3
1
2_
2
26
25
29
27
50 Days
28
24
28
26
0
0
0_
0
1
0
0__
0
14
19
21
18
Sample + Resin
14 Days
65
64
64
4
4_
4
8
8_
8
60
67
63
27 Days
45
48
46
1
1_
1
2
1_
2
57
59
58
50 Days
33
32
32
2
2_
2
2
3_
3
53
54
54
-------
Table C.8. (continued)
Initial
DRP
(ygP/1) after incubation
DRP Sample Only
Sample
No. 35
No. 36
(UgP/1) 14 Days
47 63
66
65
mean value 64
7 9
10
11
mean value 10
27 Days
49
56
_62_
56
1
1
3
2
50 Days
37
50
39
42
1
0
.1
1
Sample +• Resin
14 Days
71
74
73
4
2
3
27 Days
61
62
62
0
__5_
3
50 Days
61
61
61
2
9
5
-------
Table C.9. DARK INCUBATION BEGUN MAY 7, 1973
Unfiltered River Water Samples
Sample
No. 40
No. 41
No. 42
No. 43
No. 44
Initial
DRP
(ligP/1)
6
mean value
4
mean value
40
mean value
38
mean value
9
mean value
DRP (ygP/1)
Sample
14 Days
5
7
7_
6
6
5
4_
5
42
42
43
42
58
62
61
60
5
7
7_
6
Only
50 Days
0
0
0_
0
1
1
2_
1
44
43
40
42
61
68
70
66
3
7
3
4
after incubation
Sample ^
14 Days
29
11
20
5
4
4
38
40
39
36
36
36
5
5
5
i- Resin
50 Days
0
0_
0
4
4
2
3
36
36
36
32
•% P
35
41
36
34
38
*>*v
37
9
4
7
7
7
-------
ON
to
Table C.10. DARK INCUBATION BEGUN MAY 31, 1973
Unfiltered River Water Samples
Initial
DRP
Sample (ygP/1)
No. 49 2
mean value
No. 50 1
mean value
No. 51 104
Mean value
DRP
(ygP/1) after
Sample Only
13 Days
9
9
-JA-
12
7
9
8
8
108
109
109 _
109
26 Days
8
9
oa=
9
5
7
4
5
102
110
107
107
50 Days
1
0
4
2
1
0
1
1
90
111
_104__
104
incubation
Sample + Resin
13 Days
5
9
7
6
4
5
110
111
110
110
26 Days 50
4
2
3
0
3 v
2
105
98
102
102
Days
1
0
1
0
0
0
94
93
94
••^^^^•B
94
-------
Table C.10. (continued)
Initial
DRP
Sample (ugP/1)
No. 52 50
mean value
No. 53 5
mean value
Table
DRP (pgP/1) after incubation
13 Days
63
63
63
63
7
7
7
7
C.ll. DARK
Sample Only
26 Days
64
62
J54
63
5
2
5
4
50 Days
58
61
60
60
1
1
1
1
INCUBATION BEGUN JUNE
Unf iltered River
Sample
13 Days 26
57
53
55
9
8
8
8, 1973
+ Resin
Days 50
52
41
MMHM^_ W
46
5
4
4
Days
41
42
41
12
4
8
Water Samples
Initial
DRP
Sample (ugP/1)
No. 54 40
mean value
DRP (ygP/1) after
13 Days
49
46
48
48
Sample Only
25 Days
50
49
_A9
49
50 Days
42
40
_43_
42
incubation
Sample
13 Days 25
49
50
50
+ Resin
Days 50
49
52
•^•••^^•i •
51
Days
53
47
50
-------
Table C.12. DARK INCUBATION BEGUN JUNE 22, 1973
Unfiltered River Water Samples
Initial
DRP
Sample (PgP/1)
No. 56 26
mean value
No. 57 3
mean value
No. 58 49
mean value
No. 59 46
mean value
DRP (ygP/l) after incubation
13 Days
30
30
30
30
10
10
11
10
68
73
72
71
63
65
72
67
Sample Only
27 Days
28
27
24
26
5
2
' 5
4
70
65
70
68
66
67
69
67
50 Days
9
13
13
12
0
0
0
0
53
54
59
52
48
51
52
52
Sample
13 Days 27
24
26
25
5
13
9
77
77
77
56
54
55
+ Resin
Days 50
29
23
P^^OiM ^AM
26
8
6
v^»^*»^ i
\
7
75
80
•MA^V^HM* ^mm
77
58
53
^•••^^^H ^^
55
-
Days
12
11
^MO^BPV^
12
6
6
6
58
56
V^AB*-V*A*
57
35
31
^•4«WMB
33
-------
Table C.12. (continued)
Initial
DRP
Sample (ygP/1)
No. 60 13
mean value
No. 507-13 5
mean value
DRP (ygP/1) after incubation
Sample Only
13 Days
21
24
^
24
16
16
15
16
27 Days
13
19
19
17
10
10
10
10
50 Days
2
9.
4
5
9
4
2
5
Sample + Resin
13 Days 27 Days
20 22
__!§_ 22
19 22
13 23
J6 _29
14 26
50 Days
18
18
18
23
18
20
-------
APPENDIX D
Table D.I. CHLOROFORM TREATMENT OF NEW YORK RIVER WATER SAMPLES
Initial Data, Before Chloroform Treatment
Initial DRP Initial TSP
Sample No. (ygP/1) (ygP/1) TP(ygP/l)
27 4 8 18
mean value
28 68 71 93
mean value
29 78 82 106
mean value
31 43 49 95
mean value
33 5 10 34
mean value
DRP* in Chloroform
1 Day 7
9
8
9
9
66
71
71
69
78
81
81
80
44
47
47
46
10
11
11
11
- treated
Days
10
8
11
10
44
61
74
^^••MWMl^M
63
\
80
84
83
82
47
50
51
49b
14
15
14
14
samples after:
14 Days
10
4
10
8C
31
31
.77
46°
83
88
88
86a
15 days 9 days 16 days Each value represents one test flask
-------
Table D.I. (continued)
Initial Data, Before Chloroform Treatment DRP in Chloroform - treated samples after t
Initial DRP - Initial TSP
Sample No. (UgP/1) (UgP/1) TP(ugP/l) 1 Day
34 26 26 386 32
32
32
mean value 32
35 47 52 105 60
63
62
mean value 62
36 7 12 34 . 10
11
11
mean value 11
49 2 6 51 14
13
14
mean value 14
50 1 7 26 10
12
13
mean value 12
7 Days
36
37
36
36b
65
70
67
67b
14
15
15
15b
24
19
22
22
17
16
16
16
14 Days
28
22
22
24
20
18
18
19
9 days
-------
Table D.I. (continued)
00
Initial Data, Before Chloroform Treatment
Initial DRP Initial TSP
Sample No. (ygP/1) (ygP/1) TP (ygP/1)
51 104 111 173
mean value
52 50 57 104
mean value
53 5 16 41
mean value
- 54 40 51 87
mean value
56 26 33 59
mean value
DRP in Chloroform
1 Day 7
107
113
114
111
59
62
61
61
15
15
15
15
48
49
49
49
26
27
28
27
- treated
Days
119
123
124
122
69
71
70
70
21
19
11
17
55
58
58
57
29
7
30
22d
samples after:
14 Days
117
125
122
122
69
75
73
72
24
25
15
21
56
59
59
58
30
21
31
31
8 days
-------
Table D.I. (continued)
o\
NO
Initial Data, Before Chloroform Treatment
Initial DRP Initial TSP
Sample No. (ugP/1) (ugP/1) TP (ygP/1)
57 3 7 86
mean value
58 49 58 204
mean value
59 46 59 147
mean value
60 13 24 99
mean value
DRP in Chloroform -
1 Day 8
15
17
18
17
62
65
62
63
77
77
77
77
25
29
28
28
• treated
Days
18
18
23
20
75
76
76
76
\
86
90
27
68
9
35
39
28
samples after:
14 Days
21
21
25
22
74
80
78
77
103
93
78
91
32
40
37
37
-------
Table D.I. CHLOROFORM TREATMENT OF NEW YORK RIVER WATERS
Begun May 8, 1973 and August 13, 1973
A. Initial Data, before
Sample No.
40 Niagara R.
(Beaver I.)
41 Niagara R.
(Ft. Niagara)
42 Genesee R.
43 Oswego R.
44 Black R.
Chloroform treatment
Initial DRP
(ygP/1)
6
4
40
38
9
Initial TSP
(ygp/D
10
8
45
46
15
TP (ugP/1)
15
22
150
96
34
B. Storage of Samples without Chloroform at 4 C for 96 days (begun May
8, 1973)
Sample No.
40
41
42
43
44
DRP
Initial
6
mean value
4
mean value
40
mean value
38
mean value
9
mean value
(ygp/D
After Storage*
7
7
7
16
16
16
40
40
40
69
69
69
9
9
9
DRP after storage
(percent of TP)
47
73
27
72
26
replicate samples from cubitainer used for cold storage of bulk sample
70
-------
Table D.I. (continued)
C. Chloroform Test at 20°C
DRP in
(begun August 13,
1973)
Chloroform-Treated Samples DRP at 14 Days
Sample No. 1 Day 7 Days
40 9
10
10
10
•*•«•••
nean value 10
41 17
17
19
19
••^•MM
: mean value 19
42 43
46
44
46
^•••MMM
mean value 44
43 71
72
74
74
••M^^M
mean value 73
44 11
11
11
11
••M^HHM
mean value 11
17
10
11
11
12
19
19
20
20
20
48
51
50
50
50
71
75
76
75
74
16
16
16
16
16
14 Days (Percent of TP)
10
10
10
10
10 67
18
18
22
18
19 86
50
52
52
50
51 34
73
75
77
78
76 79
17
17
17
18
17 50
71
-------
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