EVALUATION OF FISH TISSUE DATA
FROM SELECTED SITES IN
LOUISIANA, OKLAHOMA AND TEXAS
JUNE 1996
Water Quality Protection Division
U.S. EPA, Region 6
1445 Ross Avenue
Dallas, Texas 75202-2733
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906R96103
EVALUATION OF FISH TISSUE DATA FROM
SELECTED SITES IN LOUISIANA, OKLAHOMA AND TEXAS
June r.996
Carl Young
Water Quality Protection Division
U.S. EPA, Region 6
1445 Ross Avenue
Dallas, Texas 75202-2733
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TABLE OF CONTENTS
Executive Summary
Introduction
Background
Purpose of the Study
Methods and Materials
Sites Selected and Fish Collected
Sampling Procedures
Chemical Analysis
Screening Levels
Screening Levels for Mercury
Screening Levels for Polychlorinated Biphenyls
Screening Levels for Polycyclic Aromatic Hydrocarbons
Results and Discussion
Sites Exceeding Screening Levels
Ouachita River, Louisiana
Red River, Louisiana
Bird Creek, Oklahoma
Angelina River, Texas
Rio Grande at El Paso, Texas
San Antonio River, Texas
FDA Criteria for Edible Fish Tissue
Toxicity of Polychlorinated Biphenyls
Mercury Contamination of Fish Tissue
Waterbodies where Bioaccumulation may be a Concern
References
Appendices
Appendix 1 - Sample Collection Information
Appendix 2 - Analytical Results
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EXECUTIVE SUMMARY
Edible fish tissue from 11 sites, (eight waterbodies), in
Louisiana, Oklahoma and Texas was analyzed for bioaccumulative
pollutants. The fish samples were collected in 1990 and 1991 by
the Texas Water Commission, the Louisiana Department of
Environmental Quality, the Oklahoma Water Resources Board and the
Oklahoma State Department of Health. The pollutants analyzed
included 22 pesticide components, 2.0 polychlorinated biphenyls
(PCBs) and 44 polycyclic aromatic hydrocarbons. Mercury was the
only metal analyzed.
The purpose of this one-time screening study was to identify
waterbodies where elevated levels of toxic pollutants in edible
fish tissue may be a concern. Fish tissue data was evaluated
with State specific screening levels calculated using recent EPA
guidance.
Screening levels were exceeded at 9 of 11 sites. Total PCB
screening levels were exceeded at / of the 9 sites. Mercury
screening levels were also exceeded at 7 of the 9 sites, while
the dieldrin screening level was exceeded at 1 site.
Six waterbodies were identified where elevated levels of
toxic pollutants may be a concern. These waterbodies are:
• The Ouachita River in Louisiana (mercury
and PCBs)
• The Red River in Louisiana (mercury and
PCBs)
• Bird Creek in Oklahoma (PCBs)
• The Angelina River above Lake Sam Rayburn
(mercury and PCBs)
• The Rio Grande at El Paso (PCBs)
• The San Antonio River, downstream of San
Antonio at Elmendorf, Texas (mercury, PCBs
and dieldrin)
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INTRODUCTION
Background
As a followup to EPA's National Study of Chemical Residues
in Fish, (EPA 1992), edible fish tissue from 11 sites, (eight
waterbodies), in Louisiana, Oklahoma and Texas were analyzed for
bioaccumulative pollutants. The fish samples were collected in
1990 and 1991 by the Texas Water Commission, the Louisiana
Department of Environmental Quality the Oklahoma Water Resources
Board and the Oklahoma State Department of Health.
Originally, edible fish samples were to be analyzed by
contractors at the EPA laboratory in Duluth, Minnesota.
Following problems with the analytical contract, the samples were
transferred to the Geochemical and Environmental Research Group,
(GERG), at Texas A&M University where the samples were analyzed.
Edible fish tissue samples analyzed were composites of 2 to 11
fish of the same species.
The study was conducted in resporse to concerns over human
health risks from toxic pollutants in edible fish tissue. Fish
are an important human exposure route for toxic pollutants.
These pollutants can bioaccumulate in fish through contaminated
water, sediment and food.
Purpose of the Study
The purpose of this one-time screening study was to identify
waterbodies where elevated levels of toxic pollutants in edible
fish tissue may be a concern. Steps taken to achieve this
purpose were 1) collection of composite samples of several fish
species; 2) chemical analysis of edible tissue samples; and 3)
evaluation of resultant data using pollutant specific screening
levels.
MATERIALS AND METHODS
Site Selected and Fish Collected
Waterbodies to be sampled were selected because of concerns
about potential for bioaccumulation from point sources and
nonpoint sources of pollution. Sites were selected by State and
EPA staff.
Eleven sites were sampled for fish; five in Louisiana, two
in Oklahoma and four in Texas. Game or commercial fish were
targeted for collection. Samples of three different species of
fish were targeted for collection from each site. The actual
number of species collected per site ranged from one to four.
Fish samples collected were composites of 2 to 11 fish of one
species. Table 1 lists sites and fish samples that were
collected. Appendix 1 provides detailed information on the fish
collected for analysis.
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Table 1. Fish Samples Collected
Ouachita River downstream of Louisiana/Arkansas State Line
3 White Crappie (Pomoxis annularis)
3 Smallmouth Buffalo (Ictiobus bubalus)
3 Channel Catfish (Ictalurus punctatus)
Ouachita River at Monroe, Louisiana
4 Carp (Cyprinus carpio)
3 Largemouth Bass (Micropterus salmoides)
3 Channel Catfish (Ictalurus punctatus)
Red River at Shreveport, Louisiana
4 Striped Bass (Morone saxatilis)
4 Smallmouth Buffalo (Ictiobus bubalus)
Red River downstream of Shreveport, Louisiana
4 White Bass (Morone chrysops)
3 Smallmouth Buffalo (Ictiobus bubalus)
5 Channel Catfish (Ictalurus punctatus)
Red River near Natchitoches, Louisiana
5 White Bass (Morone chrysops)
4 Bigmouth Buffalo (Ictiobus cyprinellus)
4 Channel Catfish (Ictalurus punctatus)
Arcadia Lake near Central State Park Area, Oklahoma
7 Largemouth Bass (Micropterus salmoides)
7 White Crappie (Pomoxis annularis)
5 Black Bullhead (Ictalurus melas)
Bird Creek downstream of Mingo Creek, Oklahoma
5 River Carpsucker (Carpets carpio)
2 Carp (Cyprinus carpio)
4 White Crappie (Pomoxis annularis)
2 White Bass (Morone chrysops)
Angelina River upstream of Lake Sam Rayburn, Texas
5 Spotted Gar (Lepisosteus oculatus)
3 Largemouth Bass (Micropterus salmoides)
4 Freshwater Drum (Aplodinotus grunniens)
4 White Crappie (Pomoxis annularis)
Arroyo Colorado, Texas
2 White Crappie (Pomoxis annularis)
Rio Grande at El Paso, Texas
11 Longear Sunfish (Lepomis megalotis)
5 River Carpsucker (Carpoides carpio)
San Antonio River downstream of San Antonio at Elmendorf, Texas
2 Largemouth Bass (Micropterus salmoides)
3 Longnose Gar (Lepisosteus osseus)
2 Redhorse Sucker (Moxostoma conqestum)
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Sampling Procedures
Fish were collected by electroshocking, netting (gill nets
and hoop nets) and by hook and line fishing. Fish selected for
analysis were measured and weighed, wrapped in aluminum foil,
labeled and frozen. A site survey form was completed by field
personnel which described the site and fish collection. Appendix
1 lists information from the site survey forms including
location, collection method and the length and weight of fish
collected.
Frozen composite samples were shipped on ice to the EPA
Laboratory in Duluth, Minnesota. Later, the samples were shipped
frozen to the GERG Laboratory at Texas A&M University in College
Station, Texas.
Chemical Analysis
Samples were processed and analyzed by the GERG Laboratory
at Texas A&M University. Samples were filleted, ground and
homogenized by the Laboratory prior to analysis.
The chemicals analyzed included 22 pesticide components, 20
polychlorinated biphenyls (PCBs) and 44 polycyclic aromatic
hydrocarbons (PAHs). Mercury was the only metal analyzed
Analytical results are listed in Appendix 2.
For analysis of pesticides, PCBs and PAHs, the tissue
samples were analyzed using the NOAA Status and Trends Method
(MacLeod et al., 1985) with minor revisions (Brooks et al., 1989;
Wade et al., 1988).
For mercury analysis, tissue samples were digested with
concentrated, high purity nitric acid, followed by cold vapor
atomic absorption spectrometry.
Screening Levels
The fish tissue data was evaluated with screening levels
developed from EPA guidance, (EPA 1995a), and FDA fish tissue
criteria. FDA criteria were used to evaluate levels of
chlordane, endrin,^Hnirex and mercury because the FDA criteria
were more stringent than screening levels calculated using EPA's
approach. While calculated screening levels were used to
evaluate DDT and its breakdown metabolites (DDE and ODD), an FDA
criterion was used to evaluate total DDTs. Screening levels used
for evaluating data are listed in tables 2-4. The units for
screening levels are parts per million (ppm) or milligrams per
kilogram (mg/kg).
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Table 2. Screening Levels for Louisiana
CHEMICAL
SF
(mg/kg/day)
17
7.3
Aldrin
Benzo[a]pyrene
Biphenyl
Chlordane
DDE, ODD & DDT
4, 4'-DDE 0.34
4, 4'-ODD 0.24
4,4'-DDT 0.34
Dieldrin 16
Endrin
Heptachlor 4.5
Heptachlor Epoxide 9.1
Hexachlorobenzene 1.6
alpha Lindane 6.3
beta Lindane 1.8
gamma Lindane
Mercury
Mirex
Total PCBs 7.7
RfD
(mg/kg/day)
0.0003
O.OOC3
0.00006
SCREENING
LEVEL (ppm)
0.021
0.048
1.1
0.30 a
5 a
1.0
1.5
1.0
0.022
0.30 b
0.078
0.038
0.022
C.056
0.019
1.1
0.21
0.10 C
0.045
a. FDA criteria, 55 FR 14361.
b. FDA criteria, 52 FR 18025.
c. FDA criteria, 43 FR 14736.
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Table 3. Screening Levels for Oklahoma
CHEMICAL SF RfD
(mg/kg/day)'1 (mg/kg/day)
SCREENING
LEVEL (ppm)
Aldrin 17
Benzo[a]pyrene 7.3
Biphenyl
Chlordane
DDE, ODD & DDT
4,4'-DDE 0.34
4,4'-ODD 0.24
4,4'-DDT 0.34
Dieldrin 16
Endrin
Heptachlor 4.5
Heptachlor Epoxide 9.1
Hexachlorobenzene 1.6
alpha Lindane 6.3
beta Lindane 1.8
gamma Lindane
Mercury
Mirex
Total PCBs 7.7
0.0003
0.0003
0.00006
0.063
0.15
3.2
0.30 a
5.0 a
3.2
4.5
3.2
0.067
0.30 b
0.24
0.12
0.67
0.17
0.60
3.2
0.65
0.10 c
0.14
a. FDA criteria, 55 FR 14361.
b. FDA criteria, 52 FR 18025.
c. FDA criteria, 43 FR 14736.
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Table 4. Screening Levels for Texas
CHEMICAL SF RfD
(mg/kg/day)'1 (mg/kg/day)
SCREENING
LEVEL (ppm)
Aldrin 17
Benz o[a]pyrene 7.3
Biphenyl
Chlordane
DDE, ODD & DDT
4,4'-DDE 0.34
4,4'-ODD 0.24
4,4'-DDT 0.34
Dieldrin 16
Endrin
Heptachlor 4.5
Heptachlor Epoxide 9.1
Hexachlorobenzene 1.6
alpha Lindane 6.3
beta Lindane 1.8
gamma Lindane
Mercury
Mirex
Total PCBs 7.7
0.0003
0.0003
0.00006
5,
2,
0.041
0.096
2.1
0.30 a
0 a
,1
2.9
2.1
0.044
0.30 b
0.16
0.077
0.44
.11
.39
2.1
0.42
0.10 c
0.091
0,
0,
a. FDA criteria, 55 FR 14361.
b. FDA criteria, 52 FR 18025.
c. FDA criteria, 43 FR 14736.
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Two equations listed in the EPA guidance were used to
calculate screening levels, one for chemicals considered
carcinogens and one for chemicals considered noncarcinogens.
These equations have been widely used and also serve as the basis
for development of water quality criteria. For noncarcinogens
the equation is:
SVn = (RfD*BW)/CR
where
SVn = Screening value for a noncarcinogen (mg/kg; ppm)
RfD = Oral reference dose (mg/kg/day)
BW = Mean body weight of the general population or
subpopulation of concern (kg)
CR = Mean daily consumption rate of the species of interest
by the general population or subpopulation of concern
averaged over a 70 year lifetime (kg/day)
For carcinogens the equation is:
SVc = [(RL/SF)«BW]/CR
where
SVC = Screening value for a carcinogen (mg/kg; ppm)
RL = Maximum acceptable risk level (dimensionless)
SF = Oral cancer slope factor (mg/kg/day)"1
and BW and CR are defined above.
EPA's Integrated Risk Information System (IRIS) database is
a good source of toxicological data. IRIS was utilized to obtain
values for oral reference c'oses for noncarcinogens and oral
cancer slope factors for carcinogens.
Fish tissue consumption rates used in developing screening
levels were those used in State surface water quality standards
to develop criteria to protect human health. The Louisiana
consumption rate was 20 grams/day. The Oklahoma consumption rate
was 6.5 grams/day. The Texas consumption rate was 10 grams/day.
State health agencies in Louisiana and Texas use consumption
rates of 30 grams/day, (approximately one 1/2 pound meal per
week), in evaluating fish tissue data. Use of these higher
consumption rates would lower the screening levels by a factor of
1.5 for the Louisiana screening levels and by a factor of 3 for
the Texas screening levels. The risk level used for calculating
screening levels for carcinogens was 1 x 10"4.
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Screening Levels for Mercury
Mercury screening levels were calculated based on a RfD
value of 6 x 10"5 mg/kg/day for methylmercury. The 1995 EPA
guidance states:
The EPA has recently reevaluated the RfD for
methylmercury, primarily because of concern about
evidence that the fetus is at increased risk of adverse
neurological effects from exposure to methylmercury
(Marsh et al., 1987; Piotrowski and Inskip, 1981; HAS,
1991; WHO, 1976, 1990). On May 1, 1995, IRIS was
updated to include an oral RfD of 1 x 10~4 mg/kg/day
based on developmental neurological effects in human
infants. An oral RfD of 3 x 10~4 mg/kg/day for chronic
systemic effects of methylmercury among the general
adult population was available in IRIS until May 1,
1S95; however, it was not listed in the IRIS update on
that date. For the purposes of calculating an SV
[screening value] for methylmercury that is protective
of fetuses and nursing infants, the EPA Office of Water
has chosen to continue to use the general adult
population RfD of 3 x 10"4 mg/kg/day for chronic
systemic effects of methylmercury until a value is
relisted in IRIS, and to reduce this value by a factor
of 5 to derive an RfD of 6 x 10"5 mg/kg/day for
developmental effects among infants. This factor is
based on experimental results that suggest a possible
fivefold increase in fetal sensitivity to methylmercury
exposure. This more protective approach recommended by
the EPA Office of Water was deer.ed to be most prudent
at this time. This approach should be considered
interim until such time as the Agency has reviewed new
studies on the chronic and developmental effects of
methylmercury.
Screening Levels for Polychlorinated Biphenyls
Polychlorinated biphenyls, (PCBs), have been classified as
probable human carcinogens. The cancer slope factor for Aroclor
1260 was used to calculate a screening value for total PCBs.
(Aroclor 1260 is the name of a common PCB mixture formulated for
commercial use). Screening levels for Louisiana, Oklahoma and
Texas were 0.045, 0.14 and 0.091 ppm, respectively. The FDA
tolerance level for PCBs in fish and shelDfish is 2 ppm, (21 CFR
109.30).
Screening Levels for Polvcyclic Aromatic Hydrocarbons
While several polycyclic aromatic hydrocarbons, (PAHs), have
been classified as probable human carcinogens, benzo[a]pyrene is
the only PAH for which an oral cancer slope factor is currently
listed in IRIS. Provisional EPA guidance for assessing PAHs
provides toxicity equivalence factors for six PAHs relative to
8
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benzo[a]pyrene (EPA 1993). These factors can be used to compare
a potency weighted concentration for seven PAHs with the
screening value for benzo[a]pyrene. This potency equivalency
concentration (PEC) is calculated from the equation:
PEC = S (RP«C)
where,
PEC = Potency Equivalency Concentration (ppm)
RP = Relative potency of the PAH to benzo[a]pyrene
C = Concentration of the PAH (ppm)
and relative potency factors are listed in table 5. The
screening levels for benzo[a]pyrene were also used to evaluate
potency equivalency concentrations.
Table 5. Relative Potency Factors for Selected PAHs, (EPA 1993).
COMPOUND RELATIVE POTENCY
Benzo[a]pyr ;ne 1.0
Benz[a]anthracene 0.1
Benzo[b]fluoranthene 0.1
Benzofkjfluoranthene 0.01
Chrysene 0.001
Dibenz[a,h]anthracene 1.0
Indeno[l,2,3-cd]pyrene 0.1
RESULTS AND DISCUSSION
Sites Exceeding Screening Levels
Table 6 lists sites that exceeded screening levels.
Screening levels were exceeded at 9 of 11 sites. Total PCB
screening levels were exceeded at 7 sites. Mercury screening
levels were also exceeded at 7 sites, while the dieldrin
screening level was exceeded at 1 site.
Of sites in Louisiana, screening levels for mercury and PCBs
were exceeded at two sites on the Ouachita River, and three sites
on the Red River. Screening levels for PCBs were exceeded for
samples collected from Bird Creek in Oklahoma. Of sites in
Texas, screening levels were exceeded at the Angelina River,
(mercury and PCBs), the Rio Grande, (PCBs), and the San Antonio
River, (mercury, PCBs and dieldrin). Screening levels were not
exceeded for Arcadia Lake, Oklahoma and the Arroyo Colorado,
Texas.
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Table 6. Sites Exceeding Screening Levels
SITE
CHEMICAL
FISH SPECIES
CONCENTRATION
(ppm)
Ouachita River below Mercury
the Louisiana/Arkansas Mercury
State Line Mercury
Ouachita River at
Monroe, Louisiana
Red River at
Shreveport, Louisiana
Red River below
Shreveport, Louisiana
Red River near Mercury
Natchitoches, Louisiana Mercury
Mercury
PCBs
Mercury
Mercury
PCBs
PCBs
Mercury
PCBs
Mercury
Mercury
Bird Creek below
Mingo Creek, Oklahoma
PCBs
PCBs
PCBs
Angelina River above PCBs
Lake Sam Rayburn, Texas Mercury
Mercury
Rio Grande at El Paso,
Texas
San Antonio River
below San Antonio
at Elmendorf, Texas
PCBs
PCBs
PCBs
Dieldrin
Mercury
Mercury
White Crappie 1.19
Smallmouth Buffalo 0.523
Channel Catfish 0.574
Largemouth Bass
Largemouth Bass
Channel Catfish
Striped Bass
Smallmouth Buffalo
Striped Bass
White Bass
Smallmouth Buffalo
Channel Catfish
White Bass
Bigmouth Buffalo
Channel Catfish
River Carpsucker
White Bass
Carp
Spotted Gar
Spotted Gar
Largemouth Bass
River Carpsucker
Largemouth Bass
Longnose Gar
Longnose Gar
Longnose Gar
Largemouth Bass
0.053
1.43
0.740
0.0826
0.212
0.350
0.0867
0.223
0.268
G.7S7
0.648
0.422
0.291
0.180
0.232
0.184*
0.223
0>473I
0.480*
0.111
0.421
2.98
0.0447
0.760
0.449
Mean of two analyses.
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Ouachita River. Louisiana
The two sites on the Ouachita River in Louisiana exceeded
the screening level for mercury of 0.21 ppm. At the Ouachita
River downstream of the Louisiana/Arkansas state line, the
screening levels were exceeded for white crappie (Pomoxis
annularis), smallmouth buffalo (Ictiobus bubalus) and channel
catfish (Ictalurus punctatus). The concentrations for these
species were 1.19, 0.523 and 0.574 ppm, respectively. The
average concentration for these three species was 0.762 ppm.
Additionally, the white crappie sample exceeded the FDA action
level of 1 ppm.
Further downstream at Monroe, Louisiana, largemouth bass
(Micropterus salmoides) exceeded the mercury screening level and
the FDA action level with a concentration of 1.43 ppm. Channel
catfish (Ictalurus punctatus) exceeded the screening level with a
concentration of 0.74 ppm. The concentration in carp (Cyprinus
carpio) was below the screening level with a concentration of
0.12 ppm. The average concentration was 0.763 ppm.
Several studies have documented elevated levels of mercury
in the Ouachita River. These studies led Louisiana and Arkansas
health agencies to issue fish consumption advisories in 1991 and
1992 (ADPCE 1994, LDEQ 1994).
Additionally, largemouth bass from Monroe exceeded the
screening level for total PCBs of 0.045 ppm. The total PCB
concentration for the sample was 0.053 ppm. Total PCB
concentrations for the other two samples were 0.027 and 0.011 ppm
for the carp and channel catfish samples, respectively. The
average concentration was 0.0301 ppm.
Fed River. Louisiana
The screening level for mercury of 0.21 ppm was exceeded at
all three sites on the Red River. At Shreveport, Louisiana,
striped bass (Morone saxatilis) exceeded the screening level,
(0.350 ppm), while smallmouth buffalo did not, (0.147 ppm). The
average concentration for two species was 0.249 ppm.
Downstream of Shreveport all three species collected
exceeded the screening level. Mercury concentrations for white
bass (Morone chrysops), smallmouth Buffalo (Ictiobus bubalus) and
channel catfish (Ictalurus punctatus) were 0.737, 0.223 and 0.268
ppm, respectively. The average concentration for three species
was 0.409 ppm.
Similarly, all three species collected near Natchitoches
exceeded the screening level. Mercury concentrations for white
bass, bigmouth buffalo (Ictiobus cyprinellus) and channel catfish
were 0.648, 0.422 and 0.291 ppm, respectively. The average
concentration for three species was 0.454 ppm.
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None of the samples from the Red River exceeded the FDA
action level for mercury of 1 ppm.
The screening level for total PCBs, (0.045 ppm), was
exceeded at two of the three sites on the Red River in Louisiana.
At Shreveport, Louisiana, screening levels were exceeded for the
two species sampled. PCB concentrations for striped bass (Morone
saxatilis). and smallmouth buffalo (Ictiobus bubalus), were 0.083
and 0.21 ppm, respectively.
Downstream of Shreveport, total PCB screening levels were
exceeded for white bass (Morone chrysops). (0.087 ppm). Total
PCB concentrations for smallmouth buffalo (Ictiobus bubalus) and
channel catfish (Ictalurus punctatus) at this site were 0.029 and
0.024 ppm, respectively. The average concentration was 0.0465
ppm.
The PCB screening level was not exceeded in the three fish
samples collected from the Red River near Natchitoches,
Louisiana. Even so, the concentration of total PCBs in white
bass, (0.044 ppm), almost equaled the screening level. Total PCB
concentrations in bigmouth buffalo (Ictiobus cyprinellus) and
channel catfish were 0.023 and 0.009 ppm, respectively.
None of the fish samples from the Red River exceeded the FDA
tolerance level for PCBs in fish and shellfish of 2 ppm.
Bird Creek. Oklahoma
Three of four samples collected from Bird Creek, Oklahoma
exceeded the screening level for total PCBs of 0.14 ppm. The
highest PCB concentration was 0.232 ppm, found in a sample of
white bass (Morone chrysops). Total PCB concentrations in river
carpsucker (Carpoides carpio) and carp (Cyprinus carpio) were
0.180 and 0.184 ppm, respectively. The screening level was not
exceeded in a sample of white crappie (Pomoxis annularis), which
contained 0.053 ppm. The average concentration of four species
was 0.162 ppm.
None of the fish samples from Bird Creek exceeded the FDA
tolerance level for PCBs in fish and shellfish of 2 ppm.
Angelina River. Texas
One of four samples collected from the Angelina River above
Lake Sam Rayburn, Texas exceeded the screening level for total
PCBs of 0.091 ppm. Total PCB levels in a sample of spotted gar
(Lepisosteus oculatus) were 0.223 ppm. Total PCB concentrations
in largemouth bass (Micropterus salmoides), freshwater drum
(Aplodinotus qrunniens) and white crappie (Pomoxis annularis)
were 0.0111, 0.0154 and 0.0074 ppm, respectively. The average
concentration of four species was 0.0642 ppm.
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Two of four samples exceeded the screening level of 0.42 ppm
for mercury. Mercury concentrations in spotted gar and
largemouth bass were 0.473 and 0.480 ppm, respectively. Mercury
concentrations in freshwater drum and white crappie were 0.263
and 0.388 ppm, respectively. The average concentration was 0.401
ppm.
A summary of fish tissue data in Texas can be found in The
State of Texas Water Quality Inventory (TNRCC 1994). Included is
a summary of fish tissue data for the Angelina River above Lake
Sam Rayburn, (Texas segment 0611). PCBs were detected in 2 of 4
samples, with a maximum concentration of 0.130 ppm. Mercury was
detected in 4 of 4 samples, with concentrations ranging from 0.12
to 0.54 ppm. (The summary did not specify species or whether the
sample was fillet or whole fish samples).
Additional PCB data indicate that concentrations downstream
in Lake Sam Rayburn fish are lower. PCBs levels in fish
collected from Lake Sam Rayburn in December, 1986 were documented
in National Study of Chemical Residues in Fish (EPA 1992) .
Concentrations were 0.00105 ppm for a fillet sample of white bass
and 0.0123 ppm for a whole fish sar.ple of channel catfish. The
State of Texas did not detect PCBs in three fish samples taken
from the lake, (TNRCC 1994, detection limit of 0.040 ppm).
Concentrations of mercury in Lake Sam Rayburn fish found in
the national study were 0.68 ppm for the white bass fillet and
0.10 ppm for the whole sample of channel catfish. Mercury
concentrations in 3 fish analyzed by the State of Texas ranged
from 0.051 to 0.372 ppm.
Rio Grande at El Paso. Texas
One of two samples collected from the Rio Grande at El Paso,
Texas exceeded the total PCBs screening level. The total PCB
concentration in a sample of river carpsucker (Carpoides carpio)
was 0.111 ppm, while the concentration in a sample of longear
sunfish (Lepomis meaalotis) was 0.0825 ppm. The average
concentration of two species was 0.0968 ppm.
Additional data from the Rio Grande in El Paso did not
confirm elevated levels of PCBs. In November 1992 carp (Cyprinus
carpio) and channel catfish (Ictalurus punctatus) were collected
at Courchesne Bridge and at Zaragosa International Bridge for
both fillet and whole fish tissue analysis. Samples were
analyzed for seven PCB mixtures, (Arochlor 1016, 1221, 1232,
1242, 1248, 1254, and 1260). Concentrations were below the
detection limit of 0.04 ppm, (Davis et al., 1994).
San Antonio River. Texas
Two of three samples collected from the San Antonio River at
Elmendorf, Texas exceeded the screening level for total PCBs.
One of the samples, longnose gar (Lepisosteus osseus), also
exceeded the FDA tolerance level for PCBs in fish and shellfish
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of 2 ppm, with a concentration of 2.98 ppm. The total PCB
concentration in a sample of largemouth bass (Micropterus
sa lino ides) was 0.421 ppm. The total PCB concentration for a
sample of redhorse sucker (Moxostoma conaestum) was 0.0774 ppm.
The average concentration of three species was 1.16 ppm.
Additional data supports the finding of elevated PCB levels.
PCB levels in fish collected in July, 1987 were 0.860 ppm for a
fillet sample of 2 longnose gar and 0.279 ppm for a whole fish
sample of 3 carp (EPA 1992). The State of Texas Water Quality
Inventory (TNRCC 1994) discussed elevated PCB levels in fish and
listed additional fish tissue data for the upper San Antonio
River, (Texas segment 1911). PCBs were detected in 6 of 13 fish
tissue samples, with a maximum concentration of 3.43 ppm.
Longnose gar and largemouth bass exceeded the mercury
screening level with concentrations of 0.760 ppm and 0.449 ppm,
respectively. The mercury concentration of the redhorse sucker
was 0.261 ppm. The average concentration was 0.490 ppm. Mercury
concentrations found in the national study were 0.66 ppm for
longnose gar (fillet) and 0.08 ppm for a whole carp sample (EPA
1992). The State of Texas detected mercury in 12 of 14 fish
samples, with a maximum concentration of 0.63 ppm (TNRCC 1994).
Longnose gar also exceeded the dieldrin screening level of
0.044 ppm with a concentration of 0.0447 ppm. Dieldrin
concentrations for largemouth bass and redhorse sucker were
0.0141 and 0.00637 ppm, respectively. The average concentration
was 0.0217 ppm.
Dieldrin concentrations found in the national study were
0.089 ppm for a fillet sample of longnose gar and 0.053 ppm for a
whole fish sample of carp (EPA 1992). The State of Texao listed
dieldrin as detected in 9 of 13 fish samples, with a maximum
concentration of 0.0710 ppm (TNRCC 1994).
FDA Criteria for Edible Fish Tissue
Fish samples from three sites exceeded FDA criteria. The
FDA action level for mercury of 1.0 ppm was exceeded at the two
Ouachita River sites, (one species for each site). The FDA
tolerance level for PCBs of 2 ppm was exceeded at the San Antonio
River for a sample of gar.
FDA criteria for edible fish tissue are often used to
evaluate fish tissue data. However, FDA criteria are developed
primarily for interstate commerce. They often take economic
impacts into account and are not always protective of local
subsistence fishermen or sports fishermen. FDA has criteria for
only 11 toxic pollutants.
14
-------
Toxicity of Polychlorinated Biphenyls
Screening levels for PCBs were exceeded at 7 of the 11 sites
sampled. The oral cancer slope factor of 7.7 (mg/kg/day) "1 used
to evaluate the data is based on testing of commercial PCB
mixture Arochlor 1260. EPA is currently updating its toxicity
assessment for PCBs and may revise the oral cancer slope factor
used to evaluate PCB data (EPA 1996). It is anticipated that the
update will be completed in September 1996 and published in a
final report titled PCBs: Cancer Dose-Response Assessment and
Application to Environmental Mixtures.
Mercury Contamination of Fish Tissue
Mercury is ubiquitous in the environment. Methylmercury,
the most toxic form of mercury, can bioaccumulate in fish tissue
up to a million times or more above concentrations found in the
water column. Elevated levels in fish tissue appear to be
widespread nationally. As of September 1994, 34 States have fish
consumption advisories due to mercury. EPA is presently
developing information on the nature and extent of mercury
contamination in fish (EPA 1995b).
Waterbodies where Bioaccumulation may be a Concern
Six waterbodies were identified where elevated levels of
toxic pollutants may be a concern. These waterbodies are:
• The Ouachita River in Louisiana (mercury
and PCBs)
• The Red River in Louisiana (mercury and
PCBs)
• Bird Creek in Oklahoma (PCBs)
• The Angelina River above Lake Sam Rayburn
(mercury and PCBs)
• The Rio Grande at El Paso (PCBs)
• The San Antonio River, downstream of San
Antonio at Elmendorf, Texas (mercury, PCBs
and dieldrin)
Identification of these waterbodies was based on at least
one fish tissue sample exceeding a pollutant specific screening
level. In considering whether to issue fish consumption
advisories, State health agencies consider additional factors
such as the amount and consistency of available data and the
amount of fishing activity. EPA will work cooperatively with
States to collect and evaluate data necessary for fish advisory
decisions.
15
-------
REFERENCES
ADPCE 1994. State of Arkansas 1994 Water Quality Inventory Report. Arkansas
Department of Pollution Control and Ecology, Little Rock, Arkansas.
Brooks, J.M., T. L. Wade, E. L. Atlas, M. C. Kennicutt II, B. J. Presley, R.
R. Fay, E. N. Powell, and G. Wolff 1989. Analysis of Bivalves and Sediments
for Organic Chemicals and Trace Elements. Third Annual Report for NOAA's
National Status and Trends Program, Contract 50-DGNC-5-00262.
Davis, J.R., L.J. Kleinsasser, R. Cantu, and K. Saunders 1994. Occurrence and
Impact of Toxic Chemicals in the Rio Granite and Selected Tributaries, Texas
and Mexico. Texas Natural Resource Conservation Commission, Austin, Texas.
EPA 1992. National Study of Chemical Residues in Fish, volumes 1 and 2.
September 1992. EPA 923-R92-008a and b.
EPA 1993. Provisional Guidance for Quantitative Risk Assessment of Polycyclic
Aromatic Hydrocarbons. EPA/600/R-93-089.
EPA 1995a. Guidance for Assessing Chemical Contaminant Data for use in Fish
Advisories. September 1995. EPA 923-R-95-007.
EPA 1995b. Proceedings - National Forum on Mercury in Fish, September 27-29,
1994. EPf. 823-R-95-002.
EPA 1996. Notice of external review draft document titled "PCBs: Cancer Dose-
Response Assessment and Application to Environmental Mixtures". Federal
Register, volume 61, number 85, pages 19278-19279, May 1, 1996.
LDEQ 1994. 1994 Louisiana Water Quality Inventory. Louisiana Department of
Environmental Quality, Baton Rouge, Louisiana.
MacLeod, W.D., D. W. Brown, A. J. Friedman, D.G. Burrow, O. Mayes, R.W.
Pearce, C.A. Wigren, and R. G. Bogar 1985. Standard Analytical Procedures of
the NOAA National Analytical Facility 1985-1986. Extractable Toxic Organic
Compounds. 2nd Ed. U.S. Department of Commerce, NOAA/NMFS. NOAA Tech. Memo.
NMFS F/NWC-92.
Marsh, D.O., T.W. Clarkson, C. Cox, G.J. Meyers, L. Amin-Zaki and S. Al-
Tikriti 1987. Fetal methylmercury poisoning: relationship between
concentration in single strands of maternal hair and child effects. Archives
of Neurology 44:1017-1022.
NAS (National Academy of Sciences) 1991. Seafood Safety. Committee on
Evaluation of the Safety of Fishing Products, National Academy Press,
Washington, DC.
Piotrowski, J.K., and M.J. Inskip 1981. Health Effects of Mercury; A
Technical Report (1981). MARC Report Number 24. Chelsea College, University
of London.
TNRCC 1994. The State of Texas Water Quality Inventory, 1994. Texas Natural
Resource Conservation Commission, Austin, Texas.
Wade, T.L., E. L. Atlas, J. M. Brooks, M. C. Kennicutt II, R. G. Fox, J.
Sericano, B. Garcia, and D. DeFreitas 1988. NOAA Gulf of Mexico Status and
Trends Program: Trace Organic Contaminant Distribution in Sediments and
Oysters. Estuaries, 11, 171-179.
WHO (World Health Organization) 1976. Environmental Health Criteria, 1.
Mercury. Geneva, Switzerland.
WHO (World Health Organization) 1990. Environmental Health Criteria, 101:
Methylmercury. Geneva, Switzerland.
16
-------
Appendix 1
Sample Collection Information
-------
SITE NAME: Ouachita River below Arkansas State Line
COUNTY/PARRISH, STATE: Union and Morehouse Parrishes, Louisiana
LATITUDE/LONGITUDE: 33° 00' 00" 92° 04' 00"
SITE DESCRIPTION: Ouachita River from mile markers 216 to 219.
COLLECTION METHOD: Electroshockimy, hook and line.
COLLECTORS: Louisiana Department of Environmental Quality - K.
Cormier, D. McNeill, C. Bates
SAMPLING DATE: September 5, 1990, October 12, 1990 (catfish)
FISH COLLECTED:
Sample DF019422 - 3 White Crappie (Pomoxis annularis)
Length and Weight: 1) 29.2 cm, 363 g
2) 29.2 cm, 318 g
3) 29.2 01, 227 g
Sample DF019423 - 3 Smallmouth Buffalo (Ictiobus bubalus)
Length and Weight: 1) 38.7 cm, 862 g
2) 38.7 cm, 862 g
3) 34.3 cm, 544 g
Sample DF019424 - 3 Channel Catfish (Ictalurus punctatus)
Length and Weight: 1) 38.1 cm, 454 g
2) 41.9 cm, 454 g
3) 31.8 cm, 136 g
-------
SITE NAME: Ouachita River at Monroe, Louisiana
COUNTY/PARRISH, STATE: Ouachita Parrish, Louisiana
LATITUDE/LONGITUDE: 32° 32' 30" 92° 07' 30"
SITE DESCRIPTION: Ouachita River 2 miles south of Monroe and 1
mile north of D'Arbonne Bayou.
COLLECTION METHOD: Electroshocking
COLLECTORS: Louisiana Department of Environmental Quality - K.
Cormier, C. Bates, D. McNeill
SAMPLING DATE: September 4, 1990 & October 5, 1990 (catfish)
FISH COLLECTED:
Sample DF019419 - 4 Carp (Cyprinus carpio)
Length and Weight: 1) 41.9 cm, 771 g
2) 40.6 2m, 385 g
3) 40.6 cm, 771 g
4) 30.5 cm, 272 g
Sample DF019420 - 3 Largemouth Bass (Micropterus salmoides)
Length and Weight: 1) 44.5 cm, 1179 g
2) 41.9 cm, 998 g
3) 48.3 cm, 1452 g
Sample DF019421 - 3 Channel Catfish (Ictalurus punctatus)
Length and Weight: 1) 26.7 cm, 136 g
2) 35.1 cm, 408 g
3) 2S ? cm, 181 g
-------
SITE NAME: Red River at Shreveport
COUNTY/PARRISH, STATE: Caddo Parrish, Louisiana
LATITUDE/LONGITUDE: 32° 30' 30" 93° 43' 00"
SITE DESCRIPTION: Red River at Shreveport, from Twelve Mile Bayou
to about 2.5 miles below mouth of Twelve Mile Bayou
COLLECTION METHOD: Electroshocking
COLLECTORS: Louisiana Department of Environmental Quality - Tom
Hardaway, Kirk Cormier, Matt Andrus
SAMPLING DATE: September 6, 1990
FISH COLLECTED:
Sample DF019401 - 4 Striped Bass (Morone saxatilis)
Length and Weight: 1) 53.3 cm, 1219 g
2) 47.0 cm, 680 g
3) 50.1 cm, 1077 g
4) 53.3 cm, 1270 g
Sample DF019402 - 4 Smallmouth Buffalo flctiobus buvjalus)
Length and Weight: l) 27.3 cm, 226 g
2) 26.0 cm, 226 g
3) 24.7 cm, 181 g
4) 24.1 cm, 136 g
-------
SITE NAME: Red River below Shreveport, Louisiana
COUNTY/PARRISH, STATE: Red River Parrish, Louisiana
LATITUDE/LONGITUDE: 32° 11' 93° 27'
SITE DESCRIPTION: Red River at mouth of Loggy Bayou
COLLECTION METHOD: Hoop nets and gill net
COLLECTORS: Louisiana Department of Environmental Quality - Tom
Hardaway, Lewis Still
SAMPLING DATE: September 17-18, 1990
FISH COLLECTED:
Sample DF019404 - 4 White Bass (Morone chrysops)
Length and Weight 1) 34.3 cm, 567 g
2) 38.1 cm, 726 g
3) 35.5 'Jm, 680 g
4) 35.5 cm, 680 g
Sample DF019405 - 3 Smallmouth Buffalo (Ictiobus bubalus)
Length and Weight l) 33.0 cm, 454 g
2) 34.3 cm, 567 g
3) 35.6 cm, 794 g
Sample DF019406 - 5 Channel Catfish (Ictalurus punctatus)
Length and Weight 1) 29.8 cm, 227 g
2) 26.0 cm, 227 g
3) 24.7 cm, 113 g
4) 31.7 cm, 340 g
5) 33.6 cm, 340 g
-------
SITE NAME: Red River near Natchitoches, Louisiana
COUNTY/PARRISH, STATE: Natchitoches County, Louisiana
LATITUDE/LONGITUDE: 31° 50' 51" 93° 05' 45"
SITE DESCRIPTION: Red River at the mouth of Bayou Pierre
COLLECTION METHOD: Hoop nets and gill nets
COLLECTORS: Louisiana Department of Environmental Quality - Tom
Hardaway, Lewis Still
SAMPLING DATE: September 24-25, 1990
FISH COLLECTED:
Sample DF019407 - 5 White Bass (Morone chrvsops)
Length and Weight: 1) 32.4 cm, 500 g
2) 32.4 era, 544 g
3) 36.8 cm, 748 g
4) 38.1 cm, 725 g
5) 35.5 cm, 680 g
Sample DF019408 - 4 Bigmouth Buffalo flctiobus cyprinellus)
Length and Weight: 1) 28.5 cm, 368 g
2) 31.1 cm, 453 g
3) 30.4 cm, 453 g
4) 35.5 cm, 725 g
Sample DF019409 - 4 Channel Catfish (Ictalurus punctatus)
Length and Weight: 1) 36.8 cm, 453 g
2) 31.1 cm, 255 g
3) 32.4 cm, 283 g
4) 33.0 cm, 283 g
-------
SITE NAME: Arcadia Lake near Central State Park Area
COUNTY/PARRISH, STATE: Oklahoma County, Oklahoma
LATITUDE/LONGITUDE: 35° 37' 57" 97° 23' 04"
SITE DESCRIPTION: Arcadia Lake in area south of boat ramp.
COLLECTION METHOD: Gill net - 600 feet, 2" and 3" mesh.
COLLECTORS: Oklahoma State Department of Health - J. Pigg, R.
Parham, J. Wright
SAMPLING DATE: October 10-11, 1990
FISH COLLECTED:
Sample DF019810 - 7 Largemouth Bass (Micropterus salmoidesl
Length and Weight: 1) 41.9 cm, 1355 g 5) 35.6 cm, 645 g
2) 40.6 cm, 892 g 6) 38.1 cm, 753 g
3) 39.4 cm, 853 g 7) 36.8 cm, 607 g
4) 36.2 cm, 700 g
Sample DF019811 - 7 White Crappie (Pomoxis annularis)
Length and Weight: 1) 31.8 cm, 445 g 5) 26.7 cm, 222 g
2) 25.4 cm, 255 g 6) 26.7 cm, 230 g
3) 26.7 cm, 220 g 7) 24.8 cm, 199 g
4) 24.1 cm, 188 g
Sample DF019812 - 5 Black Bullhead (Ictalurus melas)
Length and Weight: 1) 31.8 cm, 389 g
2) 29.2 cm, 320 g
3) 25.4 cm, 206 g
4) 24.1 cm, 169 g
5) 29.2 cm, 255 g
-------
SITE NAME: Bird Creek downstream of Mingo Creek
COUNTY/PARRISH, STATE: Rogers County, Oklahoma
LATITUDE/LONGITUDE: 26° 12' 00" 95° 45' 30"
SITE DESCRIPTION: Bird Creek pool at highway 167 bridge.
COLLECTION METHOD: Elecroshocking
COLLECTORS: Oklahoma Water Resources Board - Chuck Warren, Phil
Moershel
SAMPLING DATE: June 20, 1991
FISH COLLECTED:
Sample Composite 1-5 River Carpsucker (Carpoides carpio)
Length and Weight: 1) 37 cm, 652 g
2) 33 cm, 510 g
3) 28 cm, 326 g
4) 26 cm, 240 g
5) 24 cm, 184 g
Sample Composite 2-2 Carp (Cyprinus carpio)
Length and Weight: 1) 53 cm, 2240 g
2) 29 cm, 312 g
Sample Composite 3-4 White Crappie (Pomoxis annularis)
Length and Weight: 1) 21 cm, 128 g
2) 24 cm, 198 g
3) 19 cm, 56 g
4) 17 cm, 56 g
Sample Composite 4-2 White Bass (Morone chrysops)
Length and Weight: 1) 24 cm, 184 g
2) 16 cm, 43 g
-------
SITE NAME: Angelina River downstream of Paper Mill Creek
COUNTY/PARRISH, STATE: Angelina and Nacogdoches Counties, Texas
LATITUDE/LONGITUDE: 31° 26' 30" 94° 37' 18"
SITE DESCRIPTION: Angelina River downstream of the confluences of
upper and lower Paper Mill Creek.
COLLECTION METHOD: Electroshocking
COLLECTORS: Texas Water Commission: Suzanne Myers, Jeff
Kirkpatrick
SAMPLING DATE: August 21, 1990
FISH COLLECTED:
Sample DF024108 - 5 Spotted Gar (Lepisosteus oculatus)
Length: 1) 48.6 cm
2) 53.5 cm
3) 52.5 cm
4) 46.2 cm
5) 57.2 cm
Sample DF019703 - 3 Largemouth Bass (Micropterus salmoides)
Length: 1) 37.8 cm
2) 35.7 cm
3) 27.3 cm
Sample DF019701 - 4 Freshwater Drum (Aplodinotus grunniens)
Length: 1) 3 3.0 cm
2) 30.4 cm
3) 22.0 cm
4) 21.0 cm
Sample DF019702 - 4 White Crappie (Pomoxis annularis)
Length: 1) 30.5 cm
2) 18.8 cm
3) 15.9 cm
4) 15.9 cm
-------
SITE NAME: Arroyo Colorado
COUNTY/PARRISH, STATE: Cameron County, Texas
LATITUDE/LONGITUDE: 26° 11' 42" . 97° 36' 06"
SITE DESCRIPTION: 0.25 miles upstream and 0.25 downstream of
bridge at the Port of Harlingen.
COLLECTION METHOD: Electroshocking
COLLECTORS: Texas Water Commission: Jeff Kirkpatrick, David
Petrick
SAMPLING DATE: July 18, 1991
FISH COLLECTED:
Sample DF019714 - 2 White Crappie (Pomoxis annularis)
Length: 1) 33 cm
2) 39 cm
-------
SITE NAME: Rio Grande in El Paso
COUNTY/PARRISH, STATE: El Paso County, Texas
LATITUDE/LONGITUDE: 31° 40' 00" 106° 20' 00"
SITE DESCRIPTION: Rio Grande upstream of the Zaragosa Bridge.
COLLECTION METHOD: Electroshocking
COLLECTORS: Texas Water Commission: Dave Buzan
SAMPLING DATE: June 6, 1990
FISH COLLECTED:
Sample DF019708 - 11 Longear Sunfish (Lepomis megalotis)
Length and Weight: 1) 12 cm, 40 g 7) 11 cm, 30 g
2) 11 cm, 40 g 8) 8 cm, 10 g
3) 10 cm, 30 g 9) 8 cm, 10 g
4) 9 cm, 10 g 10) 8 cm, 10 g
5) 9 cm, 10 g 11) 15 cm, 85 g
6) 9 cm, 10 g
Sample DF019709 - 4 River Carpsucker (Carpoides carpio)
Length and Weight: 1) 22 cm, 130 g
2) 18 cm, 80 g
3) 16 cm, 50 g
4) 15 cm, 40 g
-------
SITE NAME: San Antonio River at Elmendorf, Texas
COUNTY/PARRISH, STATE: Bexar County, Texas
LATITUDE/LONGITUDE: 29° 14' 15" 98° 21' 43"
SITE DESCRIPTION: San Antonio River at Loop 1604 bridge, 2.8
miles south south-west of Elmendorf, Texas.
COLLECTION METHOD: Electroshocking.
COLLECTORS: Texas Water Commission - Jeff Kirkpatrick, Dave
Buzan, Augie De La Cruz. EPA Region 6 - Carl Young.
SAMPLING DATE: July 30, 1990
FISH COLLECTED:
Sample DF019704 - 2 Largemouth Bass (Micropterus salmoides)
Length and Weight: 1) 36.1 cm, 666 g
2) 27.2 cm. 303 g
Sample DF019705 - 3 Longnose Gar (Lepisosteus osseus)
Length and Weight: 1) 64.5 cm. 819 g
2) 58.9 cm, 593 g
3) 73.9 cm, 1443 g
Sample DF019706 - 2 Redhorse Sucker (Moxostoma congestum)
Length and Weight: 1) 28.7 cm, 252 g
2) 29.7 cm, 312 g
-------
Appendix 2
Analytical Results
-------
^—— ^— ^^
UCKU
TEXAS A&M UNIVERSITY
Telephone: (409) 690-0095
FAX: (409) 690-0059
TELEX: 910-380-8722
Geochemical and Environmental Research Group
833 Graham Road
College Station, Texas 77845
11 January 1994
Mr. Phil Crocker
Grants and Audit Section (6M-PG)
U.S. E.P.A., Region 6
1445 Ross Avenue (Fountain Place)
Dallas, Texas 75202-2733
Dear Phil,
In reference to your letter of 2 December 1993 regarding
clarifications for the EPA fish tissue results, the following responses
to your questions as addressed are presented.
1.
used in the
The following table identifies all of the abbreviated terms
data.
TERM
LABSAMP
SRMID
SAMPSET
SAMPID
ADGDAT
HGDGDAT
SAMPTYPE
DESCRIPTION
Lab Sample No.
Sample Set Desc.
Sponsor Sample No.
Acid Dig. Date
Hg Dig. Date
Sample Type
COMMENT
SAMP
LDUP
SPKLEV
MS
SRMLEV
SRM
BLANK
Sample
Lab Duplicate
Spike Level
Matrix Spike
Standard Reference Material
Amount
Standard Reference Material
Lab Blank
-------
PON
WETWT
ADWT
HGDWT
PCTMOIS
MATXCOD
UNITTM
UNITQUAL
V
Hg
COMMENTS
LAB
PO/ Inv. No.
Wet Wt. Rcvd.
Acid Dig. DW
Hg Dig. DW
% Moisture
Sample Matrix
Cone. Units
Wet/Dry Wt.
VPPM
HgPPM
LABID
2. Pesticide, PCB and PAH analytical results are reported on
a wet weight basis.
3. The table which lists sample numbers, species, etc. has
been updated with the additional information you requested.
4. The trace metal data have been converted to a wet
weight basis and are included in the revised technical report and on
diskette in WK1 format.
5. The percent moisture values and corresponding results
for selected samples have been corrected by the trace metal lab.
6. The SRMID column identifies the specific standard
reference material and appears in the SRMID field only when used.
The HGDWT column has been deleted.
7. A narrative QA summary has been appended to the end
of this report.
8. The Q series samples, as described in the QA narrative,
indicate quality assurance samples for internal tracking.
-------
Please contact me if the above responses, clarifications, and
corrections do not fulfill your requirements for the project. I would
be pleased to assist you with any further questions you might have
in order to complete this project.
Sincerely,
jary A.
Associa'
olff, Ph.D
Research Scientist
GAW
enclosures
xc: J. Brooks, GERG
T. Wade, GERG
B.Presley/P.Boothe, OCN
C. Kennicutt.III, GERG
-------
•^^MBB ^••••1 ^••••^ I
UCRI
The Geochemical and
Environmental Research Group
Texas A&M University
EPA Region 6
Bioaccumulation Study
TRACE METALS AND ORGANICS
RESULTS FOR TISSUE SAMPLES
January 11, 1994
Technical Report #93-145
-------
QA Summary
Pesticides PCB 198 surrogate recovery for sample C10642 was
130.5%. This surrogate is not used to quantitate any of the analytes and was
probably the result of matrix interference. Recoveries of analytes in spike
blanks and matrix spikes indicate the method was in a state of control with
recoveries ranging from 61 to 128, with most analyte recoveries between
80-100%. There were matrix interferences for 2, 4 DDE (Q1269) PCB 170
(Q6269, Q6271, Q6268), and PCB 180 (Q6330). There is a contaminant
(phthalate) in procedural blank Q6270 that interfere with PCB 170.
Duplicate analyses were acceptable.
Aromatic Hydrocarbons
Surrogate recoveries for perylene D12 surrogate was 29.7% and 39.8%
for procedural blanks Q6266 and Q6270. This surrogate is only used to
quantitate perylene, which is known to be very labile. Spike matrix (Q6273)
had matrix interference with the phenanthrene D10 surrogate. For this
spike matrix chrysene D10 was used to quantitate the analytes that are not
really quantitated using phenanthrene D10. All procedural blanks were
acceptable. The % recoveries of analytes in spiked blanks and spiked
samples was 70 to 129 with most values between 80 and 120 indicating the
method was in a state-of-control. Duplicate analyses were acceptable.
-------
EPA BIOACCUMULATION SAMPLE INFORMATION
LABNO
SAMPNO
DATE COLL
DATE REC
4980
4981
4982
4983
4984
4985
4986
4987
4988
4989
4990
4991
4992
4993
10635
10636
10637
10638
10639
10640
10641
10642
10643
10644
10645
10646
10647
11922
11923
11924
11925
DF024108
DF019703
DF019701
DFO 19702 •
DF019714
DF019708
DFO 19709
DF019419
DF019420
DF019421
DF019422
DF019423
DF019424
DF019409
DF019401
DF019402
DFO 19404
DFO 19405
DF019406
DFO 19407
DFO 19408
DF019704
DF019705
DF019706
DF019810
DF019811
DF019812
Corop #1
Comp #2
Comp #3
Comp#4
8/21/90
8/21/90
8/21/90
8/21/90
7/18/90
6/6/90
6/6/90
6/6/90
9/4/90
9/4/90
9/5/90
9/5/90
9/5/90
2/24-25/90
9/6/90
9/6/90
9/17-18/90
9/17-18/90
9/17-18/90
9/24-25/90
9/24-25/90
7/30/90
7/30/90
7/30/90
10/16-17/90
10/16-17/90
10/16-17/90
6/20/90
6/20/90
6/20/90
6/20/90
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
10/28/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/23/92
12/08/92
12/08/92
12/08/92
12/08/92
SAMPLE DESCRIPTION
5 SPOTTED GAR
3 LARGEMOUTH BASS
4 FRESTHWATER DRUM
WHITE CRAPPIE
2 WHITE CRAPPIE
11 LONGEAR SUNFISH
4 RIVER CARPSUCKER
4 CARP
3 LARGEMOUTH BASS
3 CHANNEL CATFISH
3 WHITE CRAPPIE
3 SMALLMOUTH BUFFALO
3 CHANNEL CATFISH
4 CHANNEL CATFISH
4 BASS FILET
4 SM BUFFALO FILET
4 STRIPED BASS FILET
3 SM BUFFALO FILET
5 Chan Cat
5 WHITE BASS FILET
4 BM BUFFALO FILET
2 LM BASS FILET
3 LONGNOSE GAR FILET
2 REDHORSE SUCKERS FILET
7 LM BASS FILET
7 CRAPPIE FILET
5 Bl BULLHEAD FILET
5 River Carpsuckcr(1-5)
2 Cannon Carp(6,7)
4 White Crappie(8-11)
2 White Bass(12,13)
LOCATION
Angelina River, TX
Angelina River, TX
Angelina River, TX
Angelina River, TX
Arroyo Colorado, TX
Rio Grande, El Paso
Rio Grande, El Paso
Ouachita River, Monroe
Ouachita River, Monroe
Ouachita River, Monroe
Ouachita River, state line
Ouachita River, state line
Ouachita River, state line
Red River, Natch.
Red River, S.P.
Red River, S.P.
Red River, below S.P.
Red River, below S.P.
Red River, below S.P.
Red River, Natch.
Red River, Natch.
San Antonio River,Elmendorf
San Antonio River,Elmendorf
San Antonio River,Elmendorf
Arcadia Lake Central SP
Arcadia Lake Central SP
Arcadia Lake Central SP
Bird Creek, dwnstr Mingo Crk, OK
Bird Creek, dwnstr Mingo Crk, OK
Bird Creek, dwnstr Mingo Crk, OK
Bird Creek, dwnstr Mingo Crk, OK
-------
METHOD
DLDRY
4980
4980-DUP
4981
4981-DUP
4982
SPIKE
4982-SPK
4983
SPIKE
4983-SPK
4984
4985
4986
4987
4988
4989
4990
4991
4992
4993
10635
10636
10637
10637-DUP
10638
10639
SPIKE
10639-SPK
10640
10641
10642
10643
10644
10645
10645-DUP
1/11/94
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY. #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
SAMPID
-
DF024108
DF024108
DF019703
DF019703
DF019701
DF019701
DF019701
DF019702
DFO 19702
DFO 19702
DF019714
DF019708
DF019709
DF019419
DFO 19420
DF019421
DF019422
DF019423
DFO 19424
DF019409
DF019401
DF019402
DF019404
DFO 19404
DF019405
DF019406
DF019406
DF019406
DF019407
DF019408
DF019704
DF019705
DF019706
DF019810
DF019810
ADGDAT
— "
021
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
4/13/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
8/10/93
SAMPTYPE
..
SAMP
LDUP
SAMP
LDUP
SAMP
SPKLEV
MS
SAMP
SPKLEV
MS
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
LDUP
SAMP
SAMP
SPKLEV
MS
SAMP
SAMP
SAMP
SAMP
SAMP
SAMP
LDUP
PON
^ •—_•___
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
92-050-A
WETWT
•^•"^^^^^^^^^^^^^^^^
20.22
28.28
21.76
28.06
17.3
16.89
13.03
14.31
7.53
12.31
13.16
31.09
32.92
19.39
29.27
30.31
37.47
34.71
13.58
17.36
17.99
15.53
35.13
15.74
16.38
13.56
15.15
17.56
39.42
ADWT
~ '
021
0.2006
0.2242
0.204
0.2064
0.2099
0.1948
0.1963
0.1934
0.2029
0.2059
0.1961
0.2044
0.201 1
0.2101
0.205
0.1919
0.1955
0.1932
0.1941
0.1979
0.1962
0.2
0.1966
0.197
0.2
0.1971
0.1951
0.1943
0.1914
0.1937
0.2004
0.1929
PCTMCXS
— ~
003
73.59
74.54
80.10
80.15
78.32
78.27
78.20
79.25
80.88
75.14
75.84
76.55
77.34
79.16
82.00
79.21
77.77
79.20
73.12
76.61
77.27
77.27
73.47
78.71
78.71
73.76
73.99
71.68
66.93
75.97
80.26
80.26
MATXCOD
^^^^^^^^MM^^M^MI.HMMIW
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TIOOI tr~
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
Page 1 of
-------
EPA BIOACCUMULATION DATA - TRACE METALS
LABS AMP
10646
SPIKE
10646-SPK
10647
11922
11922-DUP
11923
SPKLEV
11923-SPK
11924
11924-DUP
11925
SPKLEV
11925-SPK
SRMLEV
DORM-A
DORM-B
DORM-1-AN
SRMLEV
NBS-OYS-A
NBS-OYS-D
NBS-OYS-DS
NBS-OYS-DY
NBS-OYS-AO
SRMLEV
DOLT-2-DR
DOLT-2-DX
DOLT2-AM
BLANK-B
BLANK-C
BLANK-DN
BLANK-DP
BLANK-DV
BLANK-DW
BLANK-AK
BLANK-AL
SRMD
NRCC DORM1
NRCC DORM1
NRCC DORM1
NRCC DORM1
NIST 1566a
NIST 1566a
NIST 1566a
NIST 1566a
NIST 1566a
NIST 1566a
NRCC DOLT2
NRCC DOLT2
NRCC DOLT2
NRCC OOLT2
SAMPSET
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #1
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #2
EPA/SURVEY #1
EPA/SURVEY #1
SAMPID
DF019811
DF019811
DF019811
DF019812
COMPOSITE #1
COMPOSITE #1
COMPOSITE #2
COMPOSITE #2
COMPOSITE #2
COMPOSITE #3
COMPOSITE #3
COMPOSITE #4
COMPOSITE #4
COMPOSITE #4
ADGDAT
8/10/93
8/10/93
8/10/93
8/10/93
9/28/93
9/28/93
9/28/93
9/28/93
9/28/93
9/28/93
9/28/93
9/28/93
9/28/93
9/28/93
4/13/93
4/13/93
4/13/93
9/28/93
4/13/93
4/13/93
4/13/93
8/10/93
8/10/93
9/28/93
4/13/93
8/10/93
8/10/93
9/28/93
4/13/93
4/13/93
8/10/93
8/10/93
8/10/93
8/10/93
9/28/93
9/28/93
SAMPTYPE
SAMP
SPKLEV
M3
SAMP
SAMP
LDUP
SAMP
SPKLEV
MS
SAMP
LDUP
SAMP
SPKLEV
M3
SRMLEV
SFM
SFM
SFM
SRMLEV
SFM
SFM
SFM
SFM
SFM
SRMLEV
SFM
SFM
SU
BLANK
BLANK
BLANK
BLANK
BLANK
BLANK
BLANK
BLANK
PON
92-050-A
92-050-A
92-050-A
92-050-A
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050
92-050-A
92-050-A
92-050
92-050
92-050-A
92-050-A
92-050
92-050
92-050
92-050-A
92-050-A
92-050-A
92-050-A
92-050
92-050
WETWT ADWT
37.12 0.1976
0.1979
35.44 0.1914
21.40 0.2021
21.40 0.2082
26.33 0.2056
26.33
26.33 0.2043
19.42 0.2096
19.42 0.2006
13.70 0.2103
13.70
13.70 0.2032
0.1982
0.1932
0.2107
0.1974
0.1854
0.2023
0.1928
0.1942
0.2011
0.1949
0.2003
1
1
1
1
1
1
1
1
PCTMOIS
80.87
80.87
82.39
79.81
79.81
79.32
79.32
79.32
79.56
79.56
78.39
78.39
78.39
MATXCOD
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
TISSUE
2 of 4
-------
EPA BIOACCUMUU
DATA - TRACE METALS
— ^ _
LABSAMP
METHOD
DLDRY
4980
4980-DUP
4981
4981-DUP
4982
SPIKE
4982-SPK
4983
SPIKE
4983-SPK
4984
4985
4986
4987
4988
4989
4990
4991
4992
4993
10635
10636
10637
10637-DUP
10638
10639
SPIKE
10639-SPK
10640
10641
10642
10643
10644
10645
10645-DUP
1/1 1/94
UNITTM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
UNITQUAL
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY
DRY
DRY/WET
DRY
DRY
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY
DRY
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRY/WET
Vdry
021
0.4
0.244
0.477
0.000
0.000
0.000
20.534
19.306
0.137
20.683
18.885
0.000
0.204
0.130
0.000
0.000
0.000
0.169
0.203
0.090
0.000
0.00
0.20
0.15
0.06
0.20
0.37
20.00
20.23
0.39
0.00
0.00
0.24
0.00
0.00
0.32
V wet
0.064
0.122
0.000
0.000
0.000
0.030
0.000
0.051
0.031
O.OCO
0.000
0.000
0.030
0.042
0.020
0.000
0.000
0.048
0.034
0.014
0.054
0.079
0.103
0.000
0.000
0.081
0.000
0.000
0.062
Hg dry
021
0.03
1.801
1.848
2.389
2.441
1.213
0.504
1.618
1.780
0.515
2.348
1.334
0.351
0.366
0.497
6.304
3.551
6.595
2.515
2.583
1.399
1.303
0.627
3.148
3.332
0.839
1.260
0.25
1.490
2.470
i.624
1.587
2.300
1.086
1.148
0.941
— i — ^-••— ^•••^_»— •
Hg wet
0.476
0.470
0.475
0.485
0.263
0.388
0.255
0.087
0.088
0.117
1.428
0.740
1.187
0.523
0.574
0.291
0.350
0.147
0.716
0.757
0.223
0.268
0.648
0.422
0.449
0.760
0.261
0.227
0.186
Page 3 of 4
-------
EPA BIOACCUMULATION DATA - TRACE METALS
LABSAMP
10646
SRKE
10646-SPK
10647
11922
11922-DUP
11923
SPKLEV
11923-SPK
11924
11924-DUP
11925
SPKLEV
11925-SPK
SRMLEV
DORM-A
DORM-B
DORM-1-AN
SRMLEV
NBS-OYS-A
NBS-OYS-0
NBS-OYS-DS
NBS-OYS-DY
NBS-OYS-AO
SRMLEV
DOLT-2-DR
DOLT-2-DX
DOLT2-AM
BLANK-B
BLANK-C
BLANK-DN
BLANK-DP
BLANK-DV
BLANK-DW
BLANK-AK
BLANK-AL
UNITTM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
PPM
TOTMCG
TOTMCG
TOTMCG
TOTMCG
TOTMCG
TOTMCG
TOTMCG
TOTMCG
UNITQUAL
DRY/WET
DRV
DRV
DRY/WET
DRY/WET
DRY/WET
DRY/WET
DRV
DRV
DRY/WET
DRY/WET
DRY/WET
DRV
DRV
DRV
DRY
DRY
DRY
DRV
DRV
DRV
DRV
DRV
DRV
DRV
DRV
DRV
DRV
V dry
0.74
20.21
19.09
0.32
0.46
0.32
0.26
19.58
19.55
0.37
0.11
0.10
19.69
19.86
0.000
0.000
0.47
4.68
4.334
3.76
4.27
4.96
0.19
0.25
0.00
0.000
0.054
0.01
0.07
0.07
0.00
0.02
0.04
V wet Hg dry
0.141 0.950
0.25
1.173
0.056 0.961
0.093 0.476
0.064 0.473
0.054 0.489
0.979
1.466
0.076 0.264
0.023 0.260
0.021 0.898
0.738
1.494
0.798
0.819
0.753
0.704
0.0642
0.077
0.064
0.061
0.064
0.059
1.99
2.323
2.227
1.986
0.000
0.000
0
0
0
0
0.001
0.001
Hg wet
0.182
0.169
0.096
0.096
0.101
0.054
0.053
0.194
e 4 of 4
-------
EPA/STATE BIOACCUMULATICM SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE #
UNITS
PAGE n
EXTRACTION DATE
ANALYSIS DATE
DBOFBX (recov.)
PCB#103% (recov.)
PCB#198% (recov.)
TOTAL BHC'S
TOTAL CHLORDANES
TOTAL DOT'S
TOTAL PCB'S
ALPHA-BHC
HCB
BETA-BHC
GAMHA-BHC
DELTA-BHC
HEPTACHLOR
HEPTA-EPOXIDE
OXYCHLORDANE
GAMMA-CHLORDANE
ALPHA-CHLORDANE
TRANS-NOMACHLOR
CIS-NONACHLOR
ALDRIN
DIELDRIN
ENDRIN
HI REX
2.4'DDE (O.P'DOE)
4,4'DDE (P.P'DDE)
2,4'DDD (O.P'DDD)
4,4'DDO (P.P'ODD)
2.4'DDT (O.P'DDT)
4,4'DDT (P.P'DDT)
EPA
DF024108
C4980P
ug/Kg
N1221
08/17/93
09/22/93
74.9%
74.3%
72.7%
1.91
14.28
30.54
222.7
0.47
0.74
0.00
1.44
0.00
0.00
0.20
0.76
1.23
2.24
6.60
3.25
0.00
0.84
0.40
1.46
0.00
26.00
0.66
2.85
0.43
0.60
EPA
DF019703
C4981P
ug/Kg
M1221
08/17/93
09/22/93
70.6%
76.1%
70.8%
0.00
0.56
0.65
11.1
0.00
0.06
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.15
0.41
0.00
0.00
0.00
0.00
0.00
0.00
0.65
0.00
0.00
0.00
0.00
EPA
DF019701
C4982P
ug/Kg
M1221
08/17/93
09/22/93
73.1%
75.4%
72.1%
0.23
0.98
2.13
15.4
0.00
0.11
0.00
0.23
0.00
0.00
0.06
0.08
0.00
0.33
0.51
0.00
0.05
0.19
0.26
0.00
0.00
1.76
0.08
0.29
0.00
0.00
EPA
DF019702
C4983P
ug/Kg
M1221
08/17/93
09/22/93
73.1%
74.8%
70.0%
0.00
0.29
0.37
7.4
0.00
0.05
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.11
0.18
0.00
0.00
0.00
0.00
0.00
0.00
0.37
0.00
0.00
0.00
0.00
EPA
DF019714
C4984P
ug/Kg
M1221
08/17/93
09/22/93
71.2%
75.1%
72.1%
0.30
5.85
127.24
56.6
0.00
0.08
0.04
0.26
0.00
0.00
0.13
0.09
1.33
2.38
1.91
0.00
0.00
4.57
2.59
0.00
2.83
109.40
1.58
8.38
1.50
3.56
EPA
DF019708
C4985P
ug/Kg
H1221
08/17/93
09/22/93
72.3%
74.1%
71.6%
0.87
6.39
37.73
82.5
0.00
0.39
0.08
0.78
0.00
0.00
0.08
0.65
0.91
1.98
2.20
0.57
0.00
1.79
0.00
0.00
0.43
30.85
0.19
5.06
0.00
1.20
EPA
DF019709
C4986P
ug/Kg
M1221
08/17/93
09/22/93
67.8%
70.4%
70.3%
1.57
8.89
41.13
111.3
0.44
0.68
0.14
0.99
0.00
0.09
0.07
0.43
2.41
2.60
2.42
0.86
0.00
2.59
0.00
0.00
0.43
29.34
0.76
8.27
0.88
1.45
EPA
DF019419
C4987P
ug/Kg
H1221
08/17/93
09/22/93
74.2%
76.3%
75.7%
0.33
18.51
52.55
26.6
0.00
0.24
0.12
0.22
0.00
0.00
0.43
0.75
4.51
5.01
5.26
2.54
0.00
0.94
0.31
0.75
0.29
42.58
0.38
8.93
0.00
0.38
Page 1
22-Oct-93
-------
EPA/STATE BI "ACCUMULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE #
UNITS
PCB<« (CL2)
PCB#18 (CL3)
PCB028 (CL3)
PCB#44 (CL4)
PCBK52 (CL4)
PCB#66 (CL4)
PCB0101 (CL5)
PCB#105 (CL5)
PCB#1 10/77 (CL5/4)
PCB01 18/108/H9CCL5/5/6)
PCB#128 (CL6)
PCB#138 (CL6)
PCB#126 (CL5)
PCBHM53 (CL6)
PCB#170
PCBJK195 (CL8)
PCB0206 (CL9)
PCB0209 (CL10)
EPA
DF024108
C4980P
ug/Kg
0.0
0.0
1.2
0.0
4.1
0.9
6.7
10.0
4.1
8.1
2.4
16.1
0.0
28.2
0.0
14.7
5.6
1.2
1.3
0.0
EPA
DF019703
C4981P
ug/Kg
0.0
0.0
0.1
0.0
0.0
0.0
0.2
1.8
0.0
0.2
0.0
0.8
0.0
0.6
0.0
0.4
0.0
0.0
0.0
0.0
EPA
DF019701
C4982P
ug/Kg
0.0
0.0
0.2
0.1
0.7
0.0
0.1
2.9
0 0
0.2
0.0
0.7
0.0
0.7
0.0
0.4
0.0
0.0
0.1
0.0
MISCELLANEOUS
Type of matrix
Type of sample
Dry wt.
Wet wt.
Wt. units
Volume amt.
Vol. units
Wet or dry wt. analysis
Dry wt. X
Moisture X
Organism used 5
Fraction used
Lipid X
TIS
SAMP
1.383
5.249
g
WET
26.35
73.65
SPOTTED G3
4.180
TIS
SAMP
1.068
5.151
g
WET
20.73
79.27
LARGEMOUT4
0.425
T:S
SAMP
1.182
5.099
g
WET
23.19
76.81
EPA
DF019702
C4983P
ug/Kg
0.0
0.0
0.0
0.0
0.0
0.0
0.0
1.4
0.0
0.2
0.0
0.4
0.0
0.3
0.0
0.2
0.0
0.0
0.0
0.0
INFORMATION
TIS
SAMP
1.097
5.095
g
WET
21.53
78.47
EPA
DF019714
C4984P
ug/Kg
0.0
0.1
0.4
0.8
1.3
0.2
1.2
0.0
2.2
O.H
4.8
10.8
0.0
3.6
0.0
1.2
0.0
0.0
0.0
0.0
TIS
SAMP
0.994
4.933
g
WET
20.14
79.86
FRESTHWATWHITE CRAPP2 WHITE CRA11
0.704
0.276
0.265
EPA
DF019708
C4985P
ug/Kg
7.3
0.4
0.0
0.5
2.3
0.4
3.7
0.8
2.6
2.7
0.5
4.2
0.0
3.9
0.0
8.7
1.1
0.1
0.1
0.0
TIS
SAMP
1.253
5.194
g
WET
24.12
75.88
LONGEAR 4
2.116
EPA
OF019709
C4986P
ug/Kg
3.1
1.0
1.4
2.3
4.9
0.9
4.3
1.2
4.9
3.8
0.8
4.9
0.0
4.3
0.0
16.1
0.7
0.1
0.1
0.0
TIS
SAMP
1.198
4.756
g
WET
25.19
74.81
RIVER CAR
2.670
EPA
DF019419
C4987P
ug/Kg
0.0
0.0
0.2
0.5
1.4
0.2
0.0
0.5
0.7
0.5
0.7
2.7
0.0
1.8
0.0
1.5
0.8
0.1
0.1
0.0
TIS
SAMP
1.250
5.170
g
WET
24.18
75.82
4 CARP
3.593
BLANK Laboratory Blank
LDUP Lab QA Duplicate
MS Matrix Spike
BS Spiked Blank
Page 2
22-Oct-93
-------
EPA/STATE BIOACCUHULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE *
UNITS
PAGE it
EXTRACTION DATE
ANALYSIS DATE
DBOFBX (recov.)
PCB0103X (recov.)
PC8*198X (recov.)
TOTAL BHC'S
TOTAL CHLOROANES
TOTAL DOT'S
TOTAL PCB'S
ALPHA- BHC
HCB
BETA-BHC
GAMMA -BHC
DELTA-BHC
HEPTACHLOR
HEPTA-EPOXIDE
OXYCHLORDANE
GAMMA-CHLORDANE
ALPHA- CHLORDANE
TRANS-NONACHLOR
CIS-NONACHLOR
ALDRIN
DIELDRIN
ENDRIN
MI REX
2,4'DDE (O.P'DDE)
4.4'DDE (P.P'ODE)
2,4'DDO (O.P'DDD)
4,4'DDD (P.P'DDD)
2,4'DDT (O.P'DDT)
4,4'ODT (P.P'DOT)
EPA
OF019420
C4988P
ug/Kg
M1221
08/17/93
09/22/93
75. 3X
78.9X
78. 3X
0.26
8.74
44.07
53.0
0.15
0.12
0.00
0.11
0.00
0.00
0.33
0.78
0.52
1.32
4.26
1.52
0.00
1.16
0.28
2.34
0.24
31.13
0.19
6.95
0.36
5.19
EPA
DF019421
C4989P
ug/Kg
M1221
08/17/93
09/22/93
72. 2X
75. 4X
73. OX
0.00
1.81
10.68
11.2
0.00
0.11
0.00
0.00
0.00
0.00
0.10
0.13
0.26
0.38
0.66
0.28
0.00
0.20
0.00
0.31
0.00
8.20
0.00
1.79
0.00
0.69
EPA
DF019422
C4990P
ug/Kg
M1221
08/17/93
09/22/93
72. 9X
77.9X
74. 7X
0.00
0.37
1.16
6.9
0.00
0.05
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.11
0.26
0.00
0.00
0.08
0.00
0.00
0.00
0.91
0.00
0.19
0.00
0.07
EPA
DF019423
C4991P
ug/Kg
M1221
08/17/93
09/22/93
67. 1X
72. 1X
70. 3X
0.00
2.10
3.80
14.0
0.00
0.11
0.00
0.00
0.00
0.00
0.19
0.21
0.35
0.55
0.79
0.00
0.00
0.46
0.00
0.00
0.18
2.26
0.00
1.11
0.00
0.25
EPA
DF019424
C4992P
ug/Kg
H1221
08/17/93
09/22/93
71. OX
72. 8X
70. 3X
0.41
2.11
3.80
13.7
0.18
0.18
0.00
0.23
0.00
0.00
0.14
0.16
0.20
0.23
0.85
0.53
0.00
0.39
0.00
0.43
0.00
2.56
0.12
0.94
0.00
0.19
EPA
OF019409
C4993P
ug/Kg
M1221
08/17/93
09/22/93
71. 3X
74. 3X
74. 4X
0.00
2.00
37.87
9.6
/
0.00
0.12
0.00
0.00
0.00
0.00
0.00
0.00
0.47
0.40
0.79
0.33
0.00
0.30
0.00
5.92
0.00
32.57
0.17
4.20
0.00
0.94
EPA
DF019401
C10635P
ug/Kg
M1222
08/19/93
09/24/93
71. U
96. 1X
119. 7X
3.28
25.41
132.37
82.64
0.00
1.13
0.00
3.28
0.00
0.00
2.80
1.20
3.37
5.63
8.25
4.17
0.00
6.16
0.00
3.40
0.79
98.47
0.59
14.70
4.70
13.11
EPA
DFO 19402
C10636P
ug/Kg
M1222
08/19/93
09/24/93
80.8%
102. 6X
125. 1X
0.00
30.92
96.51
212.45
0.00
0.40
0.00
0.00
0.00
0.00
1.64
1.13
8.22
7.87
7.76
4.30
0.00
4.24
1.48
1.87
0.56
65.22
0.82
19.79
2.45
7.67
Page 3
22-Oct-93
-------
EPA/STATE B10ACCUMULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE it
UNITS
PCB18 (CL2)
PCB018 (CL3)
PCB028 (CL3)
PCB#44 (CL4)
PCB#52 (CL4)
PCB#66 (CL4)
PCBHM01 (CL5)
PCB
-------
EPA/STATE BIOACCUMULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE tt
UNITS
PAGE #
EXTRACTION DATE
ANALYSIS DATE
DBOFBX (recov.)
PCB#103% (recov.)
PCB*H98% (recov.)
TOTAL BHC'S
TOTAL CHLORDANES
TOTAL DOT'S
TOTAL PCB'S
ALPHA-BHC
HCB
BETA-BHC
GAMMA-BHC
DELTA-BHC
HEPTACHLOR
HEPTA-EPOXIDE
OXYCHLORDANE
GAMMA-CHLORDANE
ALPHl-CHLORDANE
TKANS-NONACHLOR
CIS-NONACHLOR
ALDRIN
OIELDRIN
ENDRIN
MI REX
2,4'DDE (O.P'ODE)
4,4'DDE (P.P'DOE)
2,4'DDD (O.P'DOD)
4,4'DDD (P.P'DOD)
2,4'DDT (O.P'DDT)
4,4'DDT (P.P'DDT)
EPA
OF019404
C10637P
ug/Kg
M1222
08/19/93
09/24/93
67.6%
89.9%
110. 2%
0.00
20.94
418.05
86.74
0.00
0.40
0.00
0.00
0.00
0.00
0.43
0.92
2.06
5.24
9.02
3.28
0.00
2.28
4.59
11.45
2.55
324.33
1.26
49.99
6.46
33.47
EPA
DF019405
C10638P
ug/Kg
H1222
08/19/93
09/24/93
88.2X
99. 2%
126.3%
0.00
4.47
103.07
28.81
0.00
0.31
0.00
0.00
0.00
0.00
0.30
0.00
0.66
1.23
1 33
0.96
0.00
0.75
1.63
2.12
0.40
79.92
0.50
14.18
1.22
6.87
EPA
DF019406
C10639P
ug/Kg
M1222
08/19/93
09/24/93
82.9%
97.3%
116.8%
0.00
5.90
100.76
24.16
0.00
0.21
0.00
0.00
0.00
0.00
0.00
0.00
1.15
1.73
2.25
0.75
0.00
0.36
1.19
7.84
0.00
78.06
0.44
15.18
0.57
6.50
EPA
DF019407
C10640P
ug/Kg
H1222
08/19/93
09/24/93
63.5%
98.9%
110.7%
c.oo
15.07
230.74
44.36
0.00
0.30
0.00
0.00
0.00
0.00
1.15
0.00
1.50
3.93
5.25
3.23
0.00
6.38
4.00
4.12
0.55
158.18
0.79
55.27
1.79
14.16
EPA
DF019408
C10641P
ug/Kg
M1222
08/19/93
09/24/93
73.8%
97.7%
110.2%
O.or
4.32
199.66
23.05
0.00
0.27
0.00
0.00
0.00
0.00
0.75
0.00
0.87
1.61
1.09
0.00
0.00
2.98
2.63
0.98
0.51
154.75
0.85
29.29
1.36
12.89
EPA
DF019704
C10642P
ug/Kg
M1222
08/19/93
09/24/93
87.1%
96.8%
130.5%
5.62
72.78
110.63
420.58
0.00
0.43
0.00
5.62
0.00
0.00
3.62
5.61
5.36
13.58
32.64
11.97
0.00
14.08
0.00
0.25
0.00
58.10
0.56
26.92
8.52
16.54
EPA
DF019705
C10643P
ug/Kg
M1222
08/19/93
09/24/93
53.7%
85.5%
85.9%
17.65
193.16
849.47
2983.15
0.00
2.52
0.00
12.85
4.80
0.00
9.80
20.28
21.18
39.80
78.85
23.26
0.00
44.70
0.00
4.05
5.36
661.79
4.36
137.04
20.00
20.92
EPA
DF019706
C10644P
ug/Kg
M1222
08/19/93
09/24/93
57.2%
93.4%
74.7%
0.00
14.42
28.13
77.38
0.00
0.00
0.00
0.00
0.00
0.00
1.94
1.61
2.25
3.84
3.35
1.44
0.00
6.37
0.00
0.00
0.00
15.70
0.24
6.75
2.63
2.82
Page 5
22-Oct-93
-------
EPA/STATE BI "ACCUMULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE #
UNITS
PCB18 (CL2)
PCBKM8 (CL3)
PCB#28 (CL3)
PCB#44 (CL4)
PCB052
PCB#195 (CL8)
PCB0206 (CL9)
PCB#209 (CL10)
EPA
DF019404
C10637P
ug/Kg
0.00
0.00
1.30
1.02
1.57
1.34
2.53
0.00
6.55
2.06
3.56
9.96
0.00
6.82
1.00
4.08
2.84
0.21
0.31
0.00
EPA
DF019405
C10638P
ug/Kg
0.00
0.00
0.00
0.00
3.88
0.00
0.92
0.00
1.07
0.21
1.40
2.73
0.00
1.63
0.82
0.57
0.00
0.00
0.00
0.00
EPA
DF019406
C10639P
ug/Kg
0.00
0.10
0.25
0.29
0.34
0.32
0.72
0.55
0.86
0.50
0.41
2.16
0.00
1.42
1.00
1.01
0.96
0.00
0.00
0.00
MISCELLANEOUS
Type of matrix
Type of sample
Dry wt.
Wet wt.
Ut. units
Volume amt.
Vol. units
Wet or dry wt. analysis
Dry wt. X
Moisture X
TIS
SAMP
1.258
5.048
9
WET
24.93
75.07
Organism used STRIPED BASSM
Fraction used
Lipid X
3.351
TIS
SAMP
1.156
5.122
9
WET
22.56
77.44
BUFFALO
1.910
TIS
SAMP
1.095
5.159
9
WET
21.23
78.77
EPA
DFO 19407
C10640P
ug/Kg
0.00
0.08
0.00
0.34
0.00
0.26
0.98
0.00
4.01
0.63
1.94
6.50
0.00
3.96
1.16
1.70
1.23
0.11
0.16
0.00
EPA
DF019408
C10641P
ug/Kg
0.00
0.00
0.00
0.37
0.00
0.00
0.00
0.00
0.00
0.00
1.98
3.77
0.00
1.51
0.71
0.41
0.78
0.00
0.00
0.00
EPA
DF019704
C10642P
ug/Kg
0.00
0.00
0.62
1.82
3.51
1.49
10.16
4.13
19.20
11.37
2.60
29.01
0.00
37.20
7.62
65.75
12.81
1.76
1.03
0.17
EPA
OF019705
C10643P
ug/Kg
0.00
0.38
4.43
4.53
7.83
13.48
47.55
65.76
59.09
122.35
31.93
191.10
0.00
340.62
134.92
282.20
85.58
14.51
12.00
2.01
EPA
DF019706
C10644P
ug/Kg
0.00
0.00
0.53
0.83
0.51
0.51
1.73
1.00
4.68
1.93
0.71
4.95
0.00
5.88
1.95
11.12
1.85
0.47
0.35
0.00
INFORMATION
TIS
SAMP
1.357
5.128
9
WET
26.46
73.54
UNKNOWN WHITE BASS BM
1.415
3.190
TIS
SAMP
1.145
5.164
g
WET
22.18
77.82
BUFFALO LM
2.464
TIS
SAMP
1.149
5.099
9
WET
22.53
77.47
TIS
SAMP
1.464
5.367
9
WET
27.27
72.73
TIS
SAMP
0.998
5.076
9
WET
19.67
80.33
BASS FILLOGNOSE GARREDHORSE SU
0.858
6.838
0.536
BLANK Laboratory Blank
LDUP Lab QA Duplicate
MS Matrix Spike
BS Spiked Blank
Page 6
22-Oct-93
-------
EPA/STATE BIOACCUMULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE *
UNITS
PAGE #
EXTRACTION DATE
ANALYSIS DATE
DBOFB% (recov.)
PCB*103X (recov.)
PCB0198X (recov.)
TOTAL BHC'S
TOTAL CHLORDANES
TOTAL DOT'S
TOTAL PCB'S
ALPHA-BHC
HCB
BETA-BHC
GAKMA-BHC
DELTA-BHC
HEPTACHLOR
HEPTA-EPOXIDE
OXYCHLORDANE
GAMMA-CHLORDANE
ALPHA -CHLORDANE
TRANS-NONACHLOR
CIS-NONACHLOR
ALDRIN
DIELDRIN
ENDRIN
HI REX
2,4'DDE (O.P'DOE)
4,4'DDE (P.P'DDE)
2,4'DDD (O.P'DDD)
4.4'DDD (P.P'DDD)
2.4'DDT (O.P'DOT)
4,4'DDT (P.P'DDT)
EPA
DF019810
C10645P
ug/Kg
HI 222
08/19/93
09/24/93
57.0%
80. 6X
61.6%
0.00
47.21
19.39
71.73
0.00
0.22
0.00
0.00
0.00
0.00
1.11
3.05
3.25
9.94
19.74
10.12
0.00
7.19
0.00
0.00
0.00
12.48
0.00
5.91
0.00
1.00
EPA
DF019811
C10646P
ug/Kg
Hi 222
08/19/93
09/24/93
74.9%
76.2%
58. 8X
0.00
7.81
2.19
11.25
0.00
0.13
0.00
0.00
0.00
0.00
0.46
0.00
1.23
2.21
2.35
1.56
0.00
1.37
0.00
0.00
0.00
1.36
0.00
0.83
0.00
0.00
EPA
DF019812
C10647P
ug/Kg
Hi 222
08/19/93
09/24/93
65.5%
70. 1X
62.2%
0.00
68.33
29.98
107.39
0.00
0.24
0.00
0.00
0.00
0.00
1.03
1.74
9.31
18.23
26.30
11.72
0.00
5.34
0.00
0.14
0.72
22.40
0.00
5.74
0.44
0.67
EPA
Composite
#1
C11922P
ug/Kg
H1231
09/09/93
10/13/93
68.4%
87. 9X
109. OX
1.14
23.56
10.07
180.0
0.00
0.16
0.00
1.14
0.00
0.00
1.C3
0.72
4.36
5.28
9.26
2.86
0.00
3.15
0.16
0.13
0.00
7.58
0.18
1.49
0.00
0.81
EPA
Composite
#2
C11923P
ug/Kg
H1231
09/09/93
10/13/93
60.8%
70. 2X
71. 9X
1.14
21.16
6.13
162.5
0.00
0.16
0.00
1.14
0.00
0.07
1.06
1.08
4.10
5.17
7.14
2.54
0.00
2.56
0.00
0.08
0.00
4.97
0.16
1.01
0.00
0.00
EPA
Composite
03
C11924P
ug/Kg
H1231
09/09/93
10/13/93
66.0%
65. 5X
52.2%
1.35
15.61
3.80
53.0
0.00
0.13
0.00
1.22
0.13
0.19
0.92
0.46
2.67
4.43
5.01
1.92
0.00
2.39
0.08
0.03
0.00
2.87
0.08
0.69
0.00
0.15
EPA
Compos i te
#4
C11925P
ug/Kg
M1231
09/09/93
10/13/93
67.4%
65.1%
54. 9X
1.38
25.61
8.06
232.0
0.00
0.15
0.00
1.38
0.00
0.10
0.95
1.18
2.59
6.45
10.68
3.67
0.00
2.43
0.00
0.07
0.00
6.59
0.17
0.83
0.00
0.48
EPA
06266P
Q6266P
ug/Kg
H1221
08/17/93
09/22/93
71.6%
75.7%
75.3%
0.00
0.00
0.06
2.3
0.00
0.03
0.00
0.00
0.00
0.00
0.00
0.00
0.00
O.Cv>
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.06
0.00
0.00
0.00
0.00
Page 7
22-Oct-93
-------
EPA/STATE BIOACCUMULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE #
UNITS
PCB#8 (CL2)
PCB#18 (CL3)
PCB128 (CL3)
PC8#44 (CL4)
PCB#52 (CL4)
PC8#66 (CL4)
PCB#101 (CL5)
PCB0105
-------
EPA/STATE BIOACCUHULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE *
UNITS
PAGE #
EXTRACTION DATE
ANALYSIS DATE
DBOFBX (recov.)
PCB#103X (recov.)
PCB
-------
EPA/STATE BIOACCUHULATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE #
UNITS
PCB#8 (CL2)
PCB#18 (CL3)
PCB428 (CL3)
PCB#44 (CL4)
PCB#52 (CL4)
PCB#66 (CL4)
PCBKM01 (CL5)
PC8#105 (CL5)
PCB#1 10/77 (CL5/4)
PCB#1 18/108/149(CL5/5/6)
PC8#128 (CL6)
PCBHM38 (CL6)
PCBKM26 (CL5)
PC80153 (CL6)
PCB0170 (CL7)
PCB0180 (CL7)
PCB#187/182/159(CL7/7/6>
PCBHM95 (CL8)
PCB0206 (CL9)
PCB0209 (CLIO)
Type of matrix
Type of sample
Dry wt.
Wet wt.
Ut. units
Volume amt.
Vol. units
Wet or dry wt. analysis
Dry wt. X
Moisture X
Organism used
Fraction used
Lipid X
EPA
06267P
06267P
ug/Kg
53.9
40.8
42.5
49.3
51.6
43.6
40.7
37.3
47.5
41.7
44.5
41.9
45.7
54.4
23.2
29.6
44.0
41.4
41.1
43.9
TIS
BS
1.000
a
WET
EPA
Q6267P
Q6267P
X
104
93
103
99
103
97
100
92
103
98
96
97
101
76
MI
94
95
95
89
99
T'S
BS
1.000
g
WET
EPA
DF019424
C4992P
Q6268P
ug/Kg
0.0
0.0
0.1
0.4
0.5
0.2
0.2
l.fl
O.J
0.6
0.1
1.0
0.0
0.9
0.0
0.3
0.0
0.0
0.6
0.0
MISCELLANEOUS
TIS
LDUP
1.126
5.215
9
WET
21.59
78.41
3 CHANNEL C3
2.418
EPA
DF019424
C4992P
06269P
ug/Kg
9.4
7.8
8.2
9.9
10.7
8.5
7.0
8.7
9.8
8.6
9.1
8.9
9.2
11.7
3.9
6.1
9.3
8.1
8.2
8.6
EPA
DF019424
C4992P
06269P
X
92
91
100
98
104
96
87
95
104
97
98
95
103
79
MI
96
102
94
86
99
EPA
Q6270P
Q6270P
ug/Kg
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
C.OO
0.00
0.00
0.00
0.00
0.00
MI
0.00
0.00
0.00
0.00
0.00
EPA
Q6271P
06271 P
ug/Kg
47.70
46.39
43.34
50.74
52.81
45.66
43.49
41.40
50.94
41.80
41.65
40.20
43.89
51.77
21.57
29.26
42.47
36.67
34.62
36.40
EPA
06271 P
06271 P
X
92
106
105
102
106
102
107
102
111
99
90
93
97
72
MI
93
92
84
75
82
INFORMATION
TIS
MS
1.057
5.110
g
WET
20.69
79.31
CHANNEL C3
1.7083
TIS
MS
1.057
5.110
g
WET
20.69
79.31
TIS
BLANK
5.125
3
WET
TIS
BS
1.000
9
WET
TIS
BS
1.000
9
WET
CHANNEL CATFISH
1.7083
BLANK Laboratory Blank
LDUP Lab QA Duplicate
MS Matrix Spike
BS Spiked Blank
Page 10
22-Oct-93
-------
EPA/STATE BIOACCUMUIATION SURVEY - PESTICIDE & PCB ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE *
UNITS
PAGE #
EXTRACTION DATE
ANALYSIS DATE
OBOFBX (recov.)
PCB0103X (recov.)
PCBHM98X (recov.)
TOTAL BHC'S
TOTAL CHLORDANES
TOTAL DOT'S
TOTAL PCB'S
ALPHA-BHC
HCB
BETA-BHC
GAMMA -BHC
DELTA-BHC
HEPTACHLOR
HEPTA-EPOXIDE
OXYCHLORDANE
GAMMA-CHLORDANE
ALPHA-CHLORDAN:
TJJANS-NONACHLOR
CIS-NONACHLOR
ALDRIN
DIELDRIN
ENDRIN
HIREX
2.4'DDE (O.P'DDE)
4,4'DDE (P.P'DDE)
2,4'DDD (O.P'DDD)
4,4'DDO (P.P'DOO)
2,4'ODT (O.P'OOT)
4,4'DDT (P.P'ODT)
EPA
DF019810
C10645P
Q6272P
ug/Kg
M1222
08/19/93
09/24/93
69. 8X
90. 2X
70. 7X
0.00
29.78
9.43
42.14
0.00
0.11
0.00
0.00
0.00
0.00
0.76
1.79
2.43
6.84
11.99
5.97
0.00
3.60
0.00
0.00
0.00
6.60
0.00
2.83
0.00
0.00
EPA
06327P
06327P
ug/Kg
H1231
09/09/93
10/13/93
63. 4X
61. 7X
52. 3X
0.00
0.00
0.00
2.3
0.00
0.02
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
EPA
06328P
Q6328P
ug/Kg
M1231
09/09/93
10/13/93
65. 5X
65. 8X
59. OX
102.61
223.73
162.12
1659.3
26.04
36.08
20.32
28.82
27.43
32.85
25.60
44.28
31.10
33.94
27.31
28.65
32.34
32.80
32.78
31.34
28.66
30.07
8.95
37.05
29.62
27.76
EPA
06328P
Q6328P
%
M1231
09/09/93
10/13/93
65. 5X
65. 8X
59. OX
94
78
90
61
88
77
88
79
97
80
93
89
87
88
87
91
92
80
87
64
91
92
87
EPA
Composite
#2
C11923P
Q6329P
ug/Kg
M1231
09/09/93
10/13/93
65. 4X
61. 7X
53. 6X
1.51
29.26
7.95
206.0
0.00
0.27
0.00
1.51
0.00
0.08
1.31
1.52
5.69
7.25
10.04
3.37
0.00
3.27
0.00
0.11
0.00
6.48
0.22
1.25
0.00
0.00
EPA
Composite
#2
C11923P
06330P
ug/Kg
M1231
09/09/93
10/13/93
67. 2X
61. 4X
47.0X
21.51
63.62
33.29
441.5
5.33
7.93
4.05
6.97
5.16
6.72
6.50
9.19
9.94
11.51
12.32
7.44
6.42
8.46
6.53
5.46
4.02
10.49
1.90
7.30
4.75
4.83
EPA
Composite
#2
C11923P
Q6330P
X
N1231
09/09/93
10/13/93
67. 2X
61.4%
47. OX
86
81
98
62
90
73
90
84
90
76
88
85
75
88
79
92
80
57
81
63
78
75
76
Page 11
22-Oct-93
-------
EPA/STATE BIOACCUMULATION SURVEY - PESTICIDE & PCS ANALYSIS
PROJECT
DESCRIPTOR
DUPLICATE OF
LAB SAMPLE #
UNITS
PCB#8 (CL2)
PCB018 (CL3)
PCB#28 (CL3)
PCB#44 (CL4)
PCB052 (CL4)
PCB#66 (CL4)
PCBKM01 (CL5)
PCB0105 (CL5)
PCB*1 10/77 (CL5/4)
PCB01 18/108/149(CL5/5/6>
PCB0128 (CL6)
PCB#138 (CL6)
PCB#126 (CIS)
PC80153 (CL6)
PC8i»170 (CL7)
PCB)»180 (CL7)
PCB#187/182/159(CL7/7/6)
PCBKM95 (CL8)
PCB0206 (CL9)
PCB#209 (CLIO)
Type of matrix
Type of sample
Dry wt.
Wet ut.
Wt. units
Volume amt.
Vol. units
Wet or dry wt. analysis
Dry wt. X
Moisture X
Organism used LM
Fraction used
Lipid X
EPA
DF019810
C10645P
06272P
ug/Kg
0.00
0.00
0.00
0.63
0.59
0.35
1.56
0.95
3.34
1.82
0.59
3.40
0.00
3.61
0.88
2.18
1.21
0.25
0.24
0.00
TIS
LDUP
1.071
5.015
9
WET
21.35
78.65
BASS FILET
0.810
EPA
06327P
06327P
ug/Kg
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
TIS
BLANK
5.121
g
WET
EPA
Q6328P
Q6328P
ug/Kg
41.2
37.2
42.4
49.7
50.5
42.9
40.8
40.2
51.7
42.2
44.6
41.6
45.3
52.7
40.7
28.3
44.5
40.2
76.1
40.9
MISCELLANEOUS
TIS
BS
1.000
g
WET
EPA
Q6328P
06328P
X
79
85
103
100
101
96
100
9°
112
100
96
97
100
73
97
90
96
92
78
92
EPA
Composite
#2
C11923P
06329P
ug/Kg
0.0
0.3
0.8
2.4
2.1
0.7
5.5
3.0
7.3
8.7
1.3
9.4
0.0
9.6
1.7
MI
2.8
0.3
0.3
0.0
EPA
Composite
#2
C11923P
06330P
ug/Kg
9.6
8.3
8.9
11.6
11.3
8.3
11.2
9.0
14.5
14.8
8.6
13.6
8.0
16.1
8.0
MI
9.7
6.8
6.1
6.6
EPA
Composite
#2
C11923P
06330P
X
93
94
102
104
98
87
90
82
98
89
83
73
89
61
81
MI
83
77
65
75
INFORMATION
TIS
BS
1.000
g
WET
TIS
LDUP
1.151
5.193
9
WET
22.17
77.83
TIS
MS
1.086
5.059
g
WET
21.47
78.53
TIS
MS
1.086
5.059
g
WET
21.47
78.53
Common CarpCotnmon CarpCommon Carp(6,7)
4.299
BLANK Laboratory Blank
LDUP Lab QA Duplicate
MS Matrix Spike
BS Spiked Blank
Page 12
22-Oct-93
-------
Page 1 of 30
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
INVEST*:
ID:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT:
WETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPH08:
PERYD12:
PHEND10:
INTFLAG:
PON.
CATNO:
DF024108
C4980
GCMS
M1221
GERG
TISSUE
5.25
1.38
83.0
75.3
73.7
55.6
79.7
M1221/2
DFO 19703
C4981
GCMS
M1221
GERG
TISSUE
5.15
1.07
83.0
75.4
72.8
58.2
80.9
M1221/2
DF019701
C4982
GCMS
M1221
GERG
TISSUE
5.10
1.18
84.4
82.1
80.0
57.1
84.9
M1221/2
D '01 9702
C4983
GCMS
M1221
GERG
TISSUE
5.10
1.10
83.6
78.0
85.7
68.7
86.0
M1221/2
DF019714
C4984
GCMS
M1221
GERG
TISSUE
4.93
0.99
76.7
77.7
75.9
60.1
79.4
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB"APPROVAL:
-------
Page 2 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHENJVNTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-DIBEN
FLUOR ANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
Bghi PERYLENE
0
OF024108
C4980
ng/g
Cone OB QUAL
9.91
9.50
7.62
9.95
0.00
2.43
0.43
16.44
11.89
3.12
0.00
0.00
11.05
2.74
0.00
0.00
0.00
0.00
2.03
0.00
0.00
0.00
2.13
0.95
0.00
0.22
0.24
0.00
0.00
0.00
0.00
0.12
0.12
0.12
0.15
0.21
0.04
0.09
0.07
0
DFO 19703
C4981
ng/g
Cone DB QUAL
4.05
4.01
3.67
5.88
3.58
1.56
0.14
2.02
2.27
0.71
0.00
0.00
4.94
1.01
0.00
0.00
0.00
0.00
0.80
0.00
0.00
0.00
1.38
0.90
0.00
0.20
0.24
0.00
0.00
0.00
0.00
0.13
0.13
0.12
0.12
0.08
0.08
0.03
0.06
0
DF019701
C4982
ng/g
Cone DB QUAL
3.84
4.34
4.19
5.74
0.00
2.16
0.16
3.05
3.04
0.00
0.00
0.00
3.62
0.81
0.93
0.00
0.00
0.00
0.72
0.00
0.00
0.00
0.94
0.42
0.00
0.15
0.09
0.00
0.00
0.00
0.00
0.05
0.05
0.05
0.12
0.08
0.05
0.06
0.04
0
DFO 19702
C4983
ng/g
Cone DB QUAL
4.42
3.36
2.29
4.84
0.00
2.00
0.08
1.35
1.83
1.09
2.32
1.89
4.60
0.85
1.00
0.99
0.00
0.00
0.58
0.39
0.00
0.00
1.41
0.63
0.00
0.11
0.35
0.00
0.00
0.00
0.00
0.07
0.07
0.07
0.15
0.11
0.09
0.04
0.12
0
DF019714
C4984
ng/g
Cone DB QUAL
4.76
5.41
5.02
7.64
5.46
1.93
0.09
1.43
1.83
1.73
2.72
3.07
4.90
0.72
1.18
1.30
0.00
0.00
0.65
1.18
1.30
0.00
3.15
1.27
0.00
0.06
0.61
0.00
0.00
0.00
0.00
0.14
0.14
0.08
0.09
0.10
0.08
0.06
0.08
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVA
/
-------
Page 3 of 30
EPA BIOACCUMULATIOM STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
Analyte (Cent)
2-METHYLNAPH
1-HETHYLNAPH
0
DF024108
C4980
ng/9
Cone DB QUAL
4.87
4.63
0
DFO 19703
C4981
ng/g
Cone DB QUAL
2.01
2.00
0
DF019701
C4982
ng/g
Cone DB QUAL
2.35
1.99
0
DF019702
C4983
ng/g
Cone DB QUAL
1.77
1.59
0
DF019714
C4984
ng/g
Cone DB QUAL
2.87
2.54
2,6-DIMETHNAPH 1.17
2,3,5-TRIMETHNAPH 1.53
1-METHYLPHEN 0.45
0.69
0.81
0.28
0.62
0.73
0.19
0.61
1.11
0.18
1.36
1.41
0.79
Surrogate Recoveries
NAPHD8:
ACEND10:
PHEND10:
CHRYD12:
PERYD12:
73.70
83.04
79.67
75.33
55.61
7^.81
82.99
80.90
75.44
58.22
80.04
84.39
84.88
82.13
57.05
85.70
83.65
85.99
78.05
68.66
75.91
76.65
79.41
77.68
60.05
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
JJin^
-------
Page 4 of 30
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
INVEST*:
ID:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT :
WETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CAT NO:
DF019708
CA985
GCMS
M1221
GERG
TISSUE
5.19
1.25
75.0
68.3
64.0
57.5
74.9
M1221/2
DF019709
C4986
GCMS
M1221
GERG
TISSUE
4.76
1.20
80.4
71.1
77.7
51.0
80.0
M1221/2
DF019419
C4987
GCMS
M1221
GERG
TISSUE
5.17
1.25
88.4
81.7
77.6
64.2
82.1
M1221/2
DF019420
C4988
GCMS
M1221
GERG
TISSUE
5.38
1.37
81.8
79.4
75.0
62.5
82.6
M1221/2
DF019421
C4989
GCMS
M1221
GERG
TISSUE
5.11
1.02
76.0
69.9
72.7
56.2
79.8
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 5 of 30
EPA BI"ACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA. Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
BghiPERYLENE
0
DF019708
C4985
ng/g
Cone DB QUAL
7.79
12.18
14.41
21.35
11.42
2.43
0.25
1.37
1.00
5.36
3.93
2.47
2.31
0.53
2.47
2.02
0.00
0.00
1.29
1.82
1.83
0.71
0.59
0.59
0.00
0.14
.0.17
0.00
0.00
0.00
0.00
0.13
0.13
0.11
0.10
0.11
0.04
0.02
0.11
0
DF019709
C4986
ng/g
Cone DB QUAL
6.87
17.77
39.49
58.64
44.79
3.54
2.28
3.79
2.44
15.28
10.34
8.13
3.43
0.35
3.45
2.46
0.00
0.00
2.13
4.09
5.72
2.94
1.11
0.67
0.00
0.27
0.19
0.00
0.00
0.00
0.00
0.08
0.08
0.10
0.20
0.09
0.08
0.04
0.12
0
OF019419
C4987
ng/g
Cone DB QUAL
5.67
7.01
3.95
7.06
9.52
1.25
0.23
0.62
1.58
1.83
0.00
0.00
1.68
0.69
1.58
1.32
0.00
0.00
0.44
0.90
0.00
0.00
1.38
1.06
0.00
0.08
0.54
0.00
0.00
0.00
0.00
0.06
0.06
0.10
0.07
0.12
0.02
0.03
0.03
0
DF019420
C4988
ng/g
Cone DB QUAL
7.87
8.02
4.56
6.67
6.46
1.33
0.27
0.42
0.53
1.86
3.60
0.00
1.23
0.45
0.54
0.79
0.00
0.00
0.36
0.00
0.00
0.00
0.68
0.85
0.00
0.09
0.29
0.00
0.00
0.00
0.00
0.05
0.05
0.09
0.08
0.14
0.04
0.04
0.05
0
DF019421
C4989
ng/g
Cone 08 QUAL
4.82
3.91
3.21
5.40
0.00
1.53
0.11
0.23
0.48
0.00
0.00
0.00
0.56
0.34
0.00
0.00
0.00
0.00
0.29
0.00
0.00
0.00
0.33
0.47
0.00
0.15
0.28
0.00
0.00
0.00
0.00
0.07
0.07
0.11
0.10
0.16
0.06
0.07
0.08
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 6 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
10:
LABSAMNO:
UNIT:
Analyte (Cont)
2-METHYLNAPH
1-METHYLNAPH
0
DF019708
C4985
ng/g
Cone DB QUAL
6.35
5.83
0
DF019709
C4986
ng/g
Cone DB QUAL
10.68
7.09
0
DF019419
C4987
ng/g
Cone DB QUAL
4.28
2.73
0
DF019420
C4988
ng/9
Cone DB QUAL
5.10
2.92
0
DF019421
C4989
ng/g
Cone DB QUAL
2.42
1.49
2,6-DIMETHNAPH 4.06
2,3,5-TRIMETHNAPH 3.58
1-METHYLPHEN 0.46
14.31
13.91
0.85
1.22
1.05
0.38
1.12
0.91
0.35
0.81
0.33
0.20
Surrogate Recoveries
NAPHD8: 63.97
ACEND10: 74.95
PHEND10: 74.93
CHRYD12: 68.33
PERY012: 57.46
77.65
80.40
80.57
71.08
50.97
77.58
88.42
82.09
81.74
64.24
74.95
81.81
82.64
79.42
62.53
72.73
75.98
79.84
69.95
56.15
LABNAME.-.GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 7 of 30
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
INVEST*:
ID:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SU8MAT:
SAMPLWT:
WE TUT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
DFOW22
C4990
GCMS
M1221
GERG
TISSUE
5.04
0.96
76.7
66.3
71.9
62.7
72.6
M1221/2
DF019423
C4991
GCMS
M1221
GERG
TISSUE
5.22
1.19
81.0
68.9
69.4
59.4
77.3
M1221/2
DF019424
C4992
GCMS
M1221
GERG
TISSUE
5.08
1.09
75.2
66.6
71.7
59.1
75.5
M1221/2
DFO 19409
C4993
GCMS
M1221
GERG
TISSUE
5.03
1.08
101.4
84.6
94.7
80.8
93.7
M1221/2
DF019401
C 10635
GCMS
.M1222
GERG
TISSUE
5.01
1.38
75.5
66.5
67.3
62.6
74.7
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 8 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-OIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
OBahANTHRA
Bghi PERYLENE
0
DF019422
C4990
ng/g
Cone DB QUAL
4.49
3.93
2.20
4.45
6.13
1.21
0.22
0.15
0.40
0.78
1.99
0.00
0.63
0.12
0.00
0.00
0.00
0.00
0.25
0.00
0.00
0.00
0.53
0.62
0.00
0.16
0.20
0.00
0.00
0.00
0.00
0.06
0.06
0.08
0.12
0.11
0.04
0.09
0.05
0
DF019423
C4991
ng/g
Cone DB QUAL
4.16
3.82
3.30
5.57
6.51
1.25
0.14
0.45
0.23
0.00
0.00
0.00
0.88
0.16
1.29
1.76
0.00
0.00
0.38
0.00
0.00
0.00
0.48
0.56
0.00
0.12
0.43
0.00
0.00
0.00
0.00
0.07
0.07
0.11
0.15
0.08
0.06
0.02
0.10
0
DF019424
C4992
ng/g
Cone DB QUAL
5.59
7.22
5.68
6.46
3.06
1.29
0.25
0.43
0.62
1.00
0.00
0.00
0.91
0.40
0.00
0.00
0.00
0.00
0.37
0.00
0.00
0.00
0.36
0.51
0.00
0.08
0.31
0.00
0.00
0.00
0.00
0.08
0.08
0.15
0.19
0.09
0.06
0.03
0.06
0
DF019409
C4993
ng/g
Cone DB QUAL
3.02
3.10
4.49
7.18
5.58
0.92
0.07
1.47
1.28
1.05
1.47
0.00
2.70
0.69
2.55
0.00
0.00
0.00
0.34
0.00
0.00
0.00
5.31
1.68
0.00
0.32
0.34
0.00
0.00
0.00
0.00
0.04
0.04
0.08
0.12
0.14
0.03
0.08
0.06
0
DF019401
C 10635
ng/g
Cone DB QUAL
7.65
12.65
9.99
13.49
6.60
1.97
0.74
1.03
1.41
1.89
5.15
4.12
2.11
0.84
3.06
2.67
5.62
0.00
0.94
0.00
5.33
5.07
0.96
0.83
0.00
0.24
0.92
0.00
0.00
0.00
0.00
0.03
0.03
0.11
0.13
0.31
0.05
0.05
0.05
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 9 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
Analytc (Cont)
2-METHYLNAPH
1-METHYLNAPH
0
DF019422
C4990
ng/g
Cone DB DUAL
2.35
1.58
0
OF019423
C4991
ng/g
Cone DB QUAL
2.26
1.56
0
DF019424
C4992
ng/g
Cone DB QUAL
4.33
2.89
0
DFO 19409
C4993
ng/g
Cone DB QUAL
1.75
1.35
0
DF019401
C 10635
ng/g
Cone DB QUAL
8.15
4.50
2,6-DIMETHNAPH 0.68
2,3,5-TRIMETHNAPH 0.33
1-METHYLPHEN 0.37
1.10
0.52
0.21
1.51
0.57
0.24
1.01
1.45
0.45
3.43
2.41
1.38
Surrogate Recoveries
NAPHD8:
ACEND10:
PHEND10:
CHRYD12:
PERYD12:
71.87
76.70
72.56
66.30
62.72
69.42
81.00
77.29
68.94
59.38
71.68
75.15
75.51
66.63
59.13
94.67
101.36
93.70
84.61
80.80
67.27
75.52
74.70
66.53
62.56
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 10 of 30
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
INVEST*:
ID:
LABSAHNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT :
SAMPLWT:
UETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
POH:
CAT NO:
OF019402
C 10636
GCMS
M1222
GERG
TISSUE
5.25
1.19
77.9
71.5
72.1
65.0
80.4
M1221/2
DF019404
C10637
GCMS
M1222
GERG
TISSUE
5.05
1.26
76.0
69.9
67.3
62.9
75.7
M1221/2
OF019405
C 10638
GCMS
Ml 222
GERG
TISSUE
5.12
1.16
77.1
71.3
73.6
62.4
70.5
M1221/2
OF019406
C 10639
GCMS
M1222
GERG
TISSUE
5.16
1.10
75.7
71.6
73.8
67.7
75.8
M1221/2
DF019407
C10640
GCMS
M1222
GERG
TISSUE
5.13
1.36
75.9
71.4
71.0
67.4
76.7
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 11 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLHORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
BghiPERYLENE
0
DF019402
C 10636
ng/g
Cone DB QUAL
9.02
18.58
12.33
17.32
11.89
1.73
1.21
1.65
2.82
3.95
6.95
4.90
4.72
1.27
3.86
3.47
4.60
0.00
1.31
4.00
8.22
5.92
2.09
1.23
0.00
0.11
0.54
0.00
0.00
0.00
0.00
0.11
0.11
0.07
0.05
0.07
0.06
0.06
0.03
0
DF019404
C 10637
ng/g
Cone DB QUAL
3.56
5.88
5.61
9.32
10.63
2.46
0.90
7.64
10.83
3.40
4.03
3.85
16.76
4.87
3.93
2.02
0.00
0.00
2.43
2.17
3.70
3.06
14.06
2.69
0.00
0.38
0.75
0.00
0.00
0.00
0.00
0.08
0.08
0.10
0.16
0.06
0.06
0.06
0.07
0
DF019405
C 10638
ng/g
Cone DB QUAL
2.78
4.80
4.47
7.89
7.49
1.98
0.25
3.00
5.06
3.42
3.61
0.00
8.48
2.78
5.93
1.72
3.91
1.19
1.23
1.73
2.93
2.34
9.35
2.45
0.00
0.19
0.46
0.00
0.00
0.00
0.00
0.04
0.04
0.05
0.09
0.05
0.03
0.11
0.06
0
DF019406
C 10639
ng/g
Cone DB QUAL
3.58
3.81
2.81
5.96
0.00
1.70
0.65
2.09
2.84
1.67
2.83
3.07
3.45
2.98
1.82
1.21
0.00
0.00
0.49
0.83
0.00
0.00
5.99
1.55
0.00
0.44
0.33
0.00
0.00
0.00
0.00
0.08
0.08
0.14
0.11
0.09
0.07
0.09
0.05
0
DFO 19407
C 10640
ng/g
Cone DB QUAL
2.68
5.42
5.27
10.95
10.48
1.75
0.34
1.15
1.85
3.01
4.12
4.72
2.52
1.28
3.42
2.53
0.00
0.00
0.71
2.02
4.41
4.27
3.20
0.97
0.00
0.16
0.29
O.Ou
0.00
0.00
0.00
0.07
0.07
0.12
0.09
0.15
0.05
0.02
0.08
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 12 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
Analyte (Cont)
2-METHYLNAPH
1-METHYLNAPH
2,6-DIMETHNAPH
2,3,5-fRIMETHNAPH
1-METHYLPHEN
Surrogate Recoveries
NAPHOS:
ACEND10:
PHEND10:
CHRYD12:
PER YD 12:
0
DF019402
C 10636
ng/g
Cone DB QUAL
11.85
6.73
3.92
4.11
1.48
72.13
77.93
80.35
71.55
64.98
0
DF019404
C10637
ng/g
Cone DB QUAL
3.64
2.24
1.75
1.65
2.02
67.28
75.98
75.75
69.89
62.95
0
DFO 19405
C 10638
ng/g
Cone DB QUAL
2.73
2.07
1.59
1.51
1.46
73.64
77.14
70.47
71.29
62.37
0
DF019406
C 10639
ng/g
Cone DB QUAL
2.37
1.44
0.86
1.03
0.56
73.77
75.72
75.85
71.56
67.71
0
DF019407
C 10640
ng/g
Cone DB DUAL
3.33
2.09
1.53
2.32
2.48
71.05
75.87
76.75
71.38
67.39
LABNAME: GERG/TAHU
DATE: 08-Oct-93
LAB APPROVAL:
X
*^z^--
-------
Page 13 of 30
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
INVEST*:
ID:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT:
WETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
DF019408
C10641
GCMS
M1222
GERG
TISSUE
5.16
1.15
87.7
82.5
80.8
72.3
87.6
M1221/2
DF019704
C 10642
GCMS
M1222
GERG
TISSUE
5.10
1.15
75.6
72.3
72.6
66.6
76.7
M1221/2
DF019705
C 10643
GCMS
M1222
GERG
TISSUE
5.37
1.46
75.8
73.6
70.7
67.0
75.2
M1221/2
DF019706
C 10644
GCMS
M1222
GERG
TISSUE
5.08
1.00
81.9
73.1
74.5
65.0
77.3
M1221/2
DF019810
C 10645
GCMS
Ml 222
GERG
TISSUE
5.05
1.09
83.1
79.6
83.1
69.2
83.0
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 14 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
D1BENZOTHIO
C1-DIBEN
C2-DIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRioENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
Bghi PERYLENE
0
DFO 19408
C10641
ng/g
Cone DB QUAL
2.51
5.91
7.20
12.55
10.08
1.69
0.18
1.23
1.58
3.72
6.07
2.92
2.84
0.99
3.07
2.31
0.00
0.00
0.87
2.46
4.15
3.25
2.96
1.03
0.00
0.12
0.30
0.00
0.00
0.00
0.00
0.05
0.05
0.06
0.18
0.10
0.04
0.05
0.06
0
DF019704
C 10642
ng/g
Cone DB QUAL
2.81
5.84
10.21
15.26
8.79
1.65
0.18
0.65
1.04
2.35
5.42
4.49
3.61
0.55
4.12
5.68
4.64
0.00
1.39
3.16
5.95
4.86
1.05
1.00
0.00
0.17
0.39
0.00
0.00
0.00
0.00
0.13
0.13
0.10
0.08
0.09
0.05
0.03
0.05
0
DF019705
C 10643
ng/g
Cone DB QUAL
3.92
10.10
10.71
12.30
5.35
2.92
0.17
1.10
1.59
4.05
14.84
8.13
2.52
0.94
7.69
5.65
0.00
0.00
1.86
5.60
12.43
11.62
0.79
0.49
0.00
0.33
0.21
0.00
0.00
0.00
0.00
0.16
0.16
0.13
0.20
0.19
0.09
0.04
0.09
0
DF019706
C 10644
ng/g
Cone DB QUAL
6.99
9.55
7.91
7.14
6.52
1.64
0.13
0.56
0.77
1.36
2.79
2.91
1.36
0.19
1.57
2.24
1.48
0.00
0.56
1.53
2.85
2.31
0.49
0.44
0.00
0.19
0.19
0.00
0.00
0.00
0.00
0.10
0.10
0.12
0.17
0.06
0.03
0.06
0.07
0
DF019810
C 10645
ng/g
Cone DB QUAL
2.30
5.21
4.84
6.88
0.00
0.65
0.12
0.25
0.41
0.00
0.00
0.00
0.50
0.45
0.00
0.00
0.00
0.00
0.14
0.00
0.00
0.00
0.35
0.56
0.00
0.10
0.14
0.00
0.00
0.00
0.00
0.14
0.14
0.09
0.09
0.08
0.06
0.05
0.02
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 15 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAHNO:
UNIT:
Analyte (Cont)
2-METHYLNAPH
1-METHYLNAPH
2,6-DIHETHNAPH
2,3,5-TRIMETHNAPH
1-METHYLPHEN
Surrogate Recoveries
NAPHDS:
ACEND10:
PHEND10:
CHRYD12:
PERYD12:
0
DF019408
C10641
ng/g
Cone DB QUAL
3.36
2.55
2.04
2.59
1.88
80.79
87.70
87.58
82.54
72.29
0
DF019704
C 10642
ng/g
Cone DB QUAL
3.55
2.29
3.33
3.12
0.98
72.56
75.55
76.71
72.30
66.63
0
DF019705
C 10643
ng/g
Cone DB QUAL
5.85
4.25
2.65
2.77
6.06
70.67
75.79
75.21
73.56
66.97
0
DFO 19706
C 10644
ng/g
Cone DB QUAL
5.94
3.61
2.60
1.49
0.53
74.49
81.90
77.32
73.06
64.99
0
DF019810
C 10645
ng/g
Cone DB QUAL
3.19
2.02
1.25
1.02
0.18
83.05
83.10
83.00
79.65
69.22
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL: v
-------
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
Page 16 of 30
INVEST0:
ID:
LABSAHNO:
METHOD:
OCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT:
WETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
DF019811
C10646
GCMS
M1222
GERG
TISSUE
5.19
1.04
73.6
72.2
71.3
64.0
73.0
M1221/2
DF019812
C10647
GCMS
M1222
GERG
TISSUE
5.08
0.95
82.8
79.1
78.3
61.1
82.7
M1221/2
PROC BLANK
-900
06266
GCMS
M1221
GERG
OCBLANK
10.00
10.00
70.8
77.0
71.8
29.7
75.1
M1221/2
SPIKED BLANK
-901
06267
GCMS
M1221
GERG
QCTISSUE
10.00
10.00
82.1
83.3
75.8
47.5
90.5
M1221/2
DUPLICATE
DF019424
Q6268
GCMS
M1221
GERG
TISSUE
5.22
1.13
87.5
82.5
76.3
65.3
83.7
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 17 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHR. 3ENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
BghiPERYLENE
0
DF019811
C 10646
ng/g
Cone DB QUAL
1.88
2.21
2.01
5.54
0.00
0.68
0.22
0.29
0.44
0.00
0.00
0.00
0.50
0.16
0.00
0.00
0.00
0.00
0.23
0.00
0.00
0.00
0.23
0.32
0.00
0.16
0.14
0.00
0.00
0.00
0.00
0.03
0.03
0.13
0.06
0.14
0.05
0.06
0.03
0
DF019812
C10647
ng/g
Cone DB QUAL
2.42
2.60
2.68
5.40
0.00
0.80
0.11
0.26
0.51
0.00
0.00
0.00
0.62
0.51
0.00
0.00
0.00
0.00
0.17
0.00
0.00
0.00
0.43
0.67
0.00
0.15
0.14
0.00
0.00
0.00
0.00
0.05
0.05
0.13
0.02
0.07
0.02
0.03
0.03
PROC BLANK
-900
Q6266
ng/g
Cone DB QUAL
0.97
0.86
0.00
0.00
0.00
0.58
0.20
0.12
0.12
0.00
0.00
0.00
0.21
0.06
0.00 .
0.00
0.00
0.00
0.06
0.00
0.00
0.00
0.19
0.16
0.00
0.04
0.14
0.00
0.00
0.00
0.00
0.03
0.03
0.05
0.07
0.04
0.02
0.03
0.04
SPIKED BLANK
-901
Q6%7
% Recov DB QUAL
114.5
NA
NA
NA
NA
121.6
92.3
98.0
112.4
NA
NA
NA
90.8
85.7
NA
NA
NA
NA
70.2
NA
NA
NA
109.5
108.3
NA
109.6
117.0
NA
NA
NA
NA
127.0
127.0
105.1
119.1
111.5
116.2
122.0
116.2
DUPLICATE
DF019424
06268
ng/g
Cone DB QUAL
4.76
6.18
4.73
7.46
4.75 •
1.49
0.14
0.18
0.49
1.25
4.19
0.00
0.82
0.47
0.00
0.00
0.00
0.00
0.32
0.00
0.00
0.00
0.44
0.59
0.00
0.17
0.51
0.00
0.00
0.00
0.00
0.11
0.11
0.08
0.16
0.07
0.11
0.07
0.09
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB-APPROVAL:
-'/^-e** ---
-------
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
Page 18 of 30,
INVEST*:
ID:
LABSAMNO:
UNIT:
Analyte (Cont)
0
DF019811
C10646
ng/g
Cone DB QUAL
DF019812
C10647
ng/g
Cone DB QUAL
PROC BLANK
-900
06266
ng/g
Cone DB QUAL
SPIKED BLANK
-901
Q6267
%
% Recov DB OUAL
DUPLICATE
DFO19424
Q6268
ng/g
Cone DB QUAL
2-METHYLNAPH
1-METHYLNAPH
1.42
0.79
1.60
1.00
0.51
0.35
128.8
125.0
3.76
2.42
2,6-DIMETHNAPH 0.68
2,3,5-TRIMETHNAPH 0.62
1-METHYLPHEN 0.25
0.92
0.47
0.19
0.26
0.20
0.12
100.0
129.1
82.8
1.41
0.79
0.26
Surrogate Recoveries
NAPHD8: 71.34
ACEND10: 73.62
PHEND10: 73.04
CHRYD12: 72.17
PERYD12: 64.01
78.29
82.81
82.66
79.14
61.13
71.82
70.81
75.12
76.97
29.68
75.81
82.15
90.47
83.31
47.52
76.35
87.47
83.71
82.53
65.34
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 19 of 30
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
INVEST*:
10:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT:
WETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
SPIKED MATRIX
DF019424
Q6269
GCMS
M1221
GERG
OCT ISSUE
5.11
1.06
83.7
82.1
73.7
61.9
91.2
M1221/2
PROC BLANK
-900
06270
GCMS
Ml 222
GERG
QCBLANK
10.00
10.00
81.4
79.9
77.7
39.8
05.3
M1221/2
SPIKED BLANK
-901
06271
GCMS
M1222
GERG
QCT ISSUE
10.00
10.00
82.9
78.0
82.8
49.7
88.4
M1221/2
DUPLICATE
DF019810
06272
GCMS
M1222
GERG
TISSUE
5.02
1.07
81.1
74.8
76.4
66.9
79.9
M1221/2
SPIKED MATRIX
DF019810
06273
GCMS
M1222
GERG
QCTISSUE
5.20
1.10
84.3
88.5
76.8
62.3
596.3 M
M1221/2
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 20 of 30
EPA BIOACCUMULAT10N STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-OIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
OBahANTHRA
BghiPERYLENE
SPIKED MATRIX
DF019424
Q6269
%
% Recov DB QUAL
105.1
NA
NA
NA
NA
116.1
93.0
94.8
109.0
NA
NA
NA
89.1
86.1
NA
NA
NA
NA
78.1
NA
NA
NA
110.2
105.0
NA
113.1
123.3
NA
NA
NA
NA
126.7
126.7
104.3
124.8
110.4
106.3
118.1
111.8
PROC BLANK SPIKED BLANK
-900 -901
06270 Q6271
ng/g %
Cone DB QUAL % Recov DB QUAL
0
0
0
0
0
.81
.71
.00
.00
.00
0.68
0.06
0.12
0.22
101.2
110.0
86.4
94.5
103.1
0.00
0.00
0.00
0.18
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
10
00
00
00
00
10
00
00
00
08
10
00
02
05
00
00
00
00
01
01
03
06
03
03
01
03
86.0
86.4
74.0
103.3
99.8
101.6
112.3
125.3
113.6
96.0
109.1
107.8
91.7
101.3
97.3
NA
NA
NA
NA
DUPLICATE SPIKED MAI
DF019810 DF019810
Q6272 Q6273
ng/g %
Cone DB QUAL % Recov DB OUAL
2
4
3
4
0
.64
.43
.66
.95
.00
1.00
0.24
0.35
0.38
NA
NA
NA
110
.8
NA
NA
NA
NA
117.3
89.8
91.8
107.4
0.00
0.00
0.00
0.44
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
43
00
00
00
00
21
00
00
00
30
55
00
10
32
00
00
00
00
13
13
12
11
15
06
0.10
0.04
96.
98.
99.
119.
116.
100.
110.
110.
110.
90.
124.
104.
81.
8
5
5
3
8
9
8
1
1
1
4
3
5
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
92.4
91.0
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 21 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
Analyte (Cont)
2-METHYLNAPH
1-METHYLNAPH
SPIKED MATRIX
DF019424
Q6269
%
% Recov DB QUA I
124.1
123.9
PROC BLANK
-900
062 70
ng/g
Cone DB QUAL
0.41
0.30
SPIKED BLANK
-901
Q6271
%
% Recov DB QUAL
126.9
122.4
DUPLICATE
DF019810
06272
ng/g
Cone DB QUAL
2.82
1.61
SPIKED MATRIX
DF019810
06273
%
% Recov DB
122.9
128.9
QUAL
2,6-DIMETHNAPH 96.7
2,3,5-TRIMETHNAPH 126.9
1-METHYLPHEN 82.1
0.25
0.15
0.12
93.3
120.2
78.9
1.16
0.71
0.31
98.2
126.7
94.0
Surrogate Recoveries
NAPHD8:
ACEND10:
PHEND10:
CHRYD12:
PERYD12:
73.70
83.70
91.19
82.12
61.87
77.69
81.37
85.31
79.92
39.77
82.76
82.92
88.44
77.97
49.69
76.36
81.07
79.89
74.78
66.87
76.79
84.29
596.34 M
88.50
62.33
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL: \
-------
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
Page 22 of 30
INVESTS:
10:
LABSAMNO:
METHOD:
QCBATCH:
LAS:
MATRIX:
SUBMAT:
SAMPLWT:
WETWT:
DRYWT:
VOL:
ACEND10:
CHRY012:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
LAB REF OIL
-700
W9265
GCMS
M1221
GERG
QCSBLANK
EV OIL
1.00
1.00
121.5
129.6
100.4
96.7
130.1
M1221/2
LAB REF OIL
-700
W9295
GCMS
M1222
GERG
QCSBLANK
EV OIL
1.00
1.00
109.7
115.3
95.5
90.1
119.7
M1221/2
COMPOSITE #1
C11922
GCMS
M1231
GERG
TISSUE
5.03
1.07
70.8
66.4
70.4
49.5
67.9
COMPOSITE #2
C11923
GCMS
M1231
GERG
TISSUE
5.35
1.18
63.7
55.5
66.5
49.1
63.9
COMPOSITE #3
C11924
. GCMS
M1231
GERG
TISSUE
5.07
1.08
61.9
52.5
60.4
50.2
62.6
M1231
M1231
M1231
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 23 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENA?;;:HYLENE
ACENAPHTHENE
FLUORENE
•C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
Bghi PERYLENE
LAB REF OIL
-700
W9265
ng/ AMPULE
Cone DB QUAL
520.72
2441.34
2295.02
1978.65
1129.09
193.86
0.97
14.07
88.71
216.12
357.94
334.45
207.49
4.39
494.95
594.63
523.56
271.12
177.73
359.43
528.20
496.80
10.69
12.55
79.68
5.26
57.90
89.01
116.43
24.95
18.42
3.90
3.90
10.07
2.22
2.09
1.01
1.82
3.78
LAB REF OIL
-700
W9295
ng/AMPULE
Cone DB QUAL
496.95
2407.37
2213.59
1926.87
1115.61
199.60
2.35
16.12
91.10
213.39
344.19
370.43
200.31
3.76
490.30
569.16
483.64
227.49
166.32
347.04
518.39
455.40
5.33
10.00
77.24
4.88
52.74
92.98
109.82
25.16
16.95
2.88
2.88
9.50
1.98
1.59
1.24
2.00
3.59
0
COMPOSITE #1
C11922
ng/g
Cone DB QUAL
6.91
15.91
8.21
7.56
0.00
1.90
0.48
2.35
0.86
0.00
0.00
0.00
0.99
0.72
0.00
0.00
0.00
0.00
0.29
0.00
0.00
0.00
0.36
0.24
0.00
0.21
0.36
0.00
0.00
0.00
0.00
0.07
0.08
0.12
0.09
0.30
0.15
0.14
0.08
0
COMPOSITE #2
C11923
ng/g
Cone DB QUAL
4.98
3.67
5.09
6.75
0.00
1.12
0.26
0.56
0.67
0.00
0.00
0.00
0.97
0.19
0.00
0.00
0.00
0.00
0.18
0.00
0.00
0.00
0.30
0.36
0.00
0.14
0.29
0.00
0.00
0.00
0.00
0.08
0.15
0.34
0.10
0.28
0.09
0.15
0.11
0
COMPOSITE #3
C11924
ng/g
Cone DB QUAL
6.58
9.83
5.28
9.26
0.00
1.41
0.43
0.63
0.50
0.00
0.00
0.00
1.93
0.68
0.00
0.00
0.00
0.00
0.36
0.00
0.00
0.00
0.64
0.56
0.00
0.10
0.58
0.00
0.00
0.00
0.00
0.09
0.10
0.17
0.22
0.32
0.20
0.09
0.10
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 24 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
Analyte (Cont)
2-METHYLNAPH
1-METHYLNAPH
LAB REF OIL
-700
W9265
ng/g
Cone DB QUAL
1315.50
1125.84
LAB REF OIL
-700
W9295
ng/g
Cone DB QUAL
1303.41
1103.96
0
COMPOSITE #1
C11922
ng/g
Cone DB QUAL
10.46
5.45
0
COMPOSITE #2
C11923
ng/g
Cone DB QUAL
2.02
1.65
0 I
COMPOSITE #?
C11924
ng/g
Cone DB QUAL
6.06
3.77
2,6-DIMETHNAPH 642.22
2,3,5-TRIMETHNAPH 418.67
1-METHYLPHEN 161.33
646.47
427.46
146.19
2.71
0.66
0.26
1.48
0.51
0.30
1.77
0.74
0.22
Surrogate Recoveries
NAPHD8:
ACEND10:
PHEND10:
CHRYD12:
PERYD12:
100.42
121.47
130.14
129.62
96.69
95.47
109.73
119.71
115.29
90.08
70.42
70.82
67.87
66.41
49.46
66.55
63.68
63.86
55.47
49.15
60.43
61.87
62.58
52.49
50.17
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
/
-------
EPA BIOACCUMULATION STUDY - GENERAL INFORMATION - M1221/2
Page 25 of 30
INVEST*:
ID:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT:
WETWT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
COMPOSITE #4
C11925
GCMS
M1231
GERG
TISSUE
5.02
1.14
67.0
62.5
72.6
48.4
65.7
PROC BLANK
-900
Q6327
GCMS
M1231
GERG
QCBLANK
10.00
10.00
68.1
59.7
65.8
28.2
63.4
SPIKED BLANK
-901
Q6328
GCMS
M1231
GERG
QCTISSUE
10.00
10.00
74 Jo
70.0
84.7
26.2
86.3
DUPLICATE
COMPOSITE #2
06329
GCMS
M1231
GERG
QCTISSUE
5.35
1.18
69.0
58.3
64.4
42.2
68.0
SPIKED MATRIX
COMPOSITE #2
Q6330
GCMS
M1231
GERG
QCTISSUE
5.06
1.09
67.8
68.2
67.1
40.7
82.0
M1231
M1231
M1231
M1231
M1231
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 26 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA - M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-OIBEN
C2-DIBEN
C3-DIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
Bghi PERYLENE
0
COMPOSITE #4
C11925
ng/g
Cone DB QUAL
8.18
12.80
5.93
0.00
0.00
1.34
0.37
0.62
0.60
0.00
0.00
0.00
1.11
0.22
0.00
0.00
0.00
0.00
0.42
0.00
0.00
0.00
0.39
0.45
0.00
0.09
0.57
0.00
0.00
0.00
0.00
0.15
0.17
0.10
0.18
0.43
0.04
0.17
0.06
PROC BLANK
-900
Q6327
ng/g
Cone DB QUAL
1.80
0.84
0.00
0.00
0.00
0.93
0.08
0.24
0.19
0.00
0.00
0.00
0.30
0.08
0.00
0.00
0.00
0.00
0.08
0.00
0.00
0.00
0.13
0.12
0.00
0.02
O.J7
0.00
0.00
0.00
0.00
0.04
0.05
0.06
0.05
0.23
0.04
0.04
0.06
SPIKED BLANK
-901
Q6328
% Recov DB QUAL
86.8
NA
NA
NA
NA
109.5
103.9
111.0
107.0
NA
NA
NA
88.4
82.6
NA
NA
NA
NA
71.7
NA
NA
NA
82.7
79.3
NA
99.5
113.8
NA
NA
NA
NA
101.8
98.2
88.2
93.0
112.4
88.5
105.6
96.0
DUPLICATE
COMPOSITE #2
06329
ng/g
Cone DB QUAL
5.22
3.74
4.00
10.75
0.00
1.32
0.12
0.76
0.61
0.00
0.00
0.00
1.09
0.51
0.00
0.00
0.00
0.00
0.28
0.00
0.00
0.00
0.35
0.46
0.00
0.45
0.47
0.00
0.00
0.00
0.00
0.07
0.08
0.13
0.44
0.41
0.09
0.07
0.13
SPIKED MATRIJ
COMPOSITE #2
Q6330
% Recov 08 QUAL
103.7
NA
NA
NA
NA
114.2
102.7
106.2
106.9
NA
NA
NA
85.6
79.7
NA
NA
NA
NA
71.8
NA
NA
NA
84.2
79.4
NA
97.7
107.3
NA
NA
NA
NA
91.7
102.1
83.7
91.8
100.4
82.5
99.8
92.6
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
/
-------
Page 27 of 30
EPA BIOACCUMULATION STUDY - AROMATIC HYDROCARBON DATA (CONT)- M1221/2
INVEST*:
ID:
LABSAMNO:
UNIT:
Analyte (Cont)
COMPOSITE #4
C11925
ng/g
Cone DB DUAL
PROC BLANK
-900
06327
ng/g
SPIKED BLANK
-901
Q6328
DUPLICATE
COMPOSITE #2
06329
ng/g
SPIKED MATRIX
COMPOSITE #2
06330
Cone DB QUAL % Recov DB QUAL
Cone DB QUAL % Recov DB QUAL
2-METHYLNAPH
1-METHYLNAPH
8.45
4.35
0.52
0.32
109.1
104.2
1.99
1.75
123.3
108.9
2,6-OIMETHNAPH 2.26
2,3,5-TRIMETHNAPH 1.03
1-METHYLPHEN 0.17
0.21
0.23
0.16
110.9
112.7
72.7
0.95
1.74
0.48
108.2
122.3
72.0
Surro-jate Recoveries
NAPHD8: 72.60
ACEND10: 67.02
PHEND10: 65.74
CHRYD12: 62.49
PERYD12: 48.35
65.79
68.09
63.37
59.72
28.22
84.73
73.97
86.31
69.96
26.17
64.43
68.99
68.00
58.33
42.19
67.08
67.81
81.98
68.18
40.73
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL: Vz^6
-------
Page 28 of 30
INVEST*:
10:
LABSAMNO:
METHOD:
QCBATCH:
LAB:
MATRIX:
SUBMAT:
SAMPLWT:
WETUT:
DRYWT:
VOL:
ACEND10:
CHRYD12:
NAPHD8:
PERYD12:
PHEND10:
INTFLAG:
PON:
CATNO:
EPA BIOACCUMULATIO
LAB REF OIL
20762
W29523
GCMS
M1231
GERG
QCSBLANK
EV OIL
1.00
1.00
113.1
113.6
93.1
116.3
116.3
M1231
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 29 of 30
EPA BIOACCUMULATIO
INVEST*:
ID:
LABSAMNO:
UNIT:
PNA Analyte
NAPHTHALENE
C1 -NAPHTHALENES
C2-NAPHTHALENES
C3-NAPHTHALENES
C4-NAPHTHALENES
BIPHENYL
ACENAPHTHYLENE
ACENAPHTHENE
FLUORENE
C1-FLUORENES
C2-FLUORENES
C3-FLUORENES
PHENANTHRENE
ANTHRACENE
C1-PHEN_ANTHR
C2-PHEN_ANTHR
C3-PHEN_ANTHR
C4-PHEN_ANTHR
DIBENZOTHIO
C1-DIBEN
C2-DIBEN
C3-OIBEN
FLUORANTHENE
PYRENE
C1-FLUORAN_PYR
BENaANTHRACENE
CHRYSENE
C1-CHRYSENES
C2-CHRYSENES
C3-CHRYSENES
C4-CHRYSENES
BENbFLUORAN
BENkFLUORAN
BENePYRENE
BENaPYRENE
PERYLENE
I123cdPYRENE
DBahANTHRA
BghiPERYLENE
LAB REF OIL
20762
W29523
ng/g
Cone OB QUAL
603.79
2402.47
2155.14
1680.26
1037.59
200.71
2.55
17.96
89.87
243.75
328.30
318.51
246.12
4.54
527.03
584.57
452.38
265.87
151.27
289.65
376.06
392.46
5.20
10.25
84.79
5.23
54.33
97.69
87.28
25.57
17.85
4.53
5.06
10.26
2.14
3.26
0.95
1.77
4.25
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
-------
Page 30 of 30
EPA BIOACCUMULATIO
INVEST*:
10:
LABSAMNO:
UNIT:
Analyte (Cont)
LAB REF OIL
20762
U29523
ng/g
Cone DB OUAL
2-METHYLNAPH 1318.49
1-METHYLNAPH 1083.98
2,6-DIMETHNAPH 773.72
2,3,5-TRIMETHNAPH 413.36
1-HETHYLPHEN 172.90
Surrogate Recoveries
NAPHD8: 93.07
ACEN010: 113.12
PHEND10: 116.31
CHRY012: 113.55
PERYD12: 116.31
LABNAME: GERG/TAMU
DATE: 08-Oct-93
LAB APPROVAL:
------- |