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              AIR POLLUTION ASPECTS

                        OF

               ORGANIC CARCINOGENS
                Prepared for the
  National Air Pollution Control Administration
Consumer Protection & Environmental Health Service
   Department of Health, Education, and Welfare
           (Contract No. PH-22-68-25)
       Compiled by Douglas A. Olsen, Ph.D.
               and James L. Haynes, M.S.
               Litton Systems, Inc0
          Environmental Systems Division
                7300 Pearl Street
             Bethesda, Maryland 20014

                  September 1969

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                          FOREWORD


       As the concern for air quality grows, so does the con-

cern over the less ubiquitous but potentially harmful contami-

nants that are in our atmosphere.  Thirty such pollutants have

been identified, and available information has been summarized

in a series of reports describing their sources, distribution,

effects, and control technology for their abatement.

       A total of 27 reports have been prepared covering the

30 pollutants.  These reports were developed under contract

for the National Air Pollution Control Administration  (NAPCA) by

Litton Systems, Inc.  The complete listing is as follows:


    Aeroallergens (pollens)       Ethylene
    Aldehydes (includes acrolein  Hydrochloric Acid
      and formaldehyde)           Hydrogen Sulfide
    Ammonia                       Iron and Its Compounds
    Arsenic and Its Compounds     Manganese and Its Compounds
    Asbestos                      Mercury and its Compounds
    Barium and Its Compounds      Nickel and Its Compounds
    Beryllium and Its Compounds   Odorous Compounds
    Biological Aerosols           Organic Carcinogens
      (microorganisms)            Pesticides
    Boron and Its Compounds       Phosphorus and Its Compounds
    Cadmium and Its Compounds     Radioactive Substances
    Chlorine Gas                  Selenium and Its Compounds
    Chromium and Its Compounds    Vanadium and Its Compounds
      (includes chromic acid)     Zinc and Its Compounds


       These reports represent current state-of-the-art

literature reviews supplemented by discussions with selected

knowledgeable individuals both within and outside the Federal

Government.  They do not however presume to be a synthesis of

available information but rather a summary without an attempt

to interpret or reconcile conflicting data.  The reports are

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necessarily limited in their discussion of health effects for

some pollutants to descriptions of occupational health expo-

sures and animal laboratory studies since only a few epidemio-

logic studies were available.

       Initially these reports were generally intended as

internal documents within NAPCA to provide a basis for sound

decision-making on program guidance for future research

activities and to allow ranking of future activities relating

to the development of criteria and control technology docu-

ments.  However, it is apparent that these reports may also

be of significant value to many others in air pollution control,

such as State or local air pollution control officials, as a

library of information on which to base informed decisions on

pollutants to be controlled in their geographic areas.  Addi-

tionally, these reports may stimulate scientific investigators

to pursue research in needed areas.  They also provide for the

interested citizen readily available information about a given

pollutant.  Therefore, they are being given wide distribution

with the assumption that they will be used with full knowledge

of their value and limitations.

       This series of reports was compiled and prepared by the

Litton personnel listed below:

       Ralph J. Sullivan
       Quade R. Stahl, Ph.D.
       Norman L. Durocher
       Yanis C. Athanassiadis
       Sydney Miner
       Harold Finkelstein, Ph.D.
       Douglas A. Olsen, Ph0D.
       James L. Haynes

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       The NAPCA project officer for the contract was Ronald C.




Campbell, assisted by Dr. Emanuel Landau and Gerald Chapman.




       Appreciation is expressed to the many individuals both




outside and within NAPCA who provided information and reviewed




draft copies of these reports.  Appreciation is also expressed




to the NAPCA Office of Technical Information and Publications




for their support in providing a significant portion of the




technical literature.

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                           ABSTRACT



        Epideitiiological studies indicate that air pollution



may play a role in lung cancer induction.  Certain organic



carcinogens, normally present in polluted air, particularly



benzo(a)pyrene, have been shown to increase tumor incidence



in experimental animals.  In addition, animal studies indi-



cate that other organic compounds may have synergistic or



antagonistic effects.  No information has been found on the



effects of organic carcinogens on plants or materials.



        Organic carcinogens fall into three main categories—



polynuclear aromatic hydrocarbons, polynuclear heterocyclic



and oxygenated compounds, and alkylating agents.  The




major emission sources of organic carcinogens, particularly



of polynuclear aromatic hydrocarbons, are heat-generation



sources, such as burning coal, oil, and gas; refuse burning;



motor vehicle exhaust; and industrial processes.  Of these,



heat generation accounts for more than 85 percent of the



polynuclear aromatic hydrocarbons generated, while the other



three sources each account for about 5 percent of the total.



        In 1966, the average ambient air concentration of



benzo(a)pyrene in the United States was approximately 0.003



ug/m3 for urban areas and 0.0003 ug/m3 for nonurban areas.



Data indicate that benzo(a)pyrene represents only a small



percentage of the total amount of polynuclear aromatic hydro-



carbons found in the atmosphere.

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        Abatement methods for organic carcinogens are



presently being studied under the hydrocarbon control pro-



grams.



        The costs of premature death and the associated



costs of treatment and burial for lung cancer ascribable to



air pollution have been estimated.  No information has been



found on the economic costs of the abatement of organic



carcinogen air pollution.



        Methods of analysis are available which involve the



use of extraction and chromatographic techniques for separa-



tion of the compounds followed by analysis with spectral



methods.

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                          CONTENTS

FOREWORD

ABSTRACT

1.  INTRODUCTION 	    1

    1.1  Types of Organic Carcinogens  	    3
         1.1.1  Polynuclear Aromatic Hydrocarbons (PAH)    3
         1.1.2  Polynuclear Heterocyclics and
                Oxygenated Compounds 	    8
         1.1.3  Alkylating Agents  	    8
    1.2  Organic Carcinogens as Cocarcinogens or
         Anticarcinogens 	   10

2.  EFFECTS	12

    2.1  Effects on Humans	14
         2.1.1  Particulate Matter 	   20
         2.1.2  Particle Size	22
         2.1.3  Irritants	25
         2.1.4  Studies with Biological Material ....   26
    2.2  Effects on Animals  .	27
         2.2.1  Commercial and Domestic Animals  ....   27
         2.2.2  Experimental Animals	28
                2.2.2.1  Effects on Animal Tissue  ...   39
    2.3  Effects on Plants	40
    2.4  Effects on Materials	40
    2.5  Environmental Air Standards .  .  .	40

3.  SOURCES	41

    3.1  Natural Occurrence  	   41
    3.2  Production Sources  .  .	42
    3.3  Product Sources	47
    3.4  Environmental Air Concentrations  	   51

4.  ABATEMENT	53

5.  ECONOMICS	•   55

6.  METHODS OF ANALYSIS	•   59

    6.1  Sampling Methods  	   59
    6.2  Extraction Methods  	   59
    6.3  Separation	61
         60 3.1  Column Chroma to graphy	61
         6.3.2  Thin-Layer Chromatography	64
         6.3.3  Gas Chromatography	66
         6.3.4  Other Techniques 	   66

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                    CONTENTS (Continued)

    6.4  Analysis	67
         6.4.1  Spectral Methods 	  67
         6.4.2  Other Methods	68

7.  SUMMARY AND CONCLUSIONS	71

REFERENCES

APPENDIX

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                       LIST OF FIGURES

1.  Retention of Particulate Matter in Lung in Relation
    to Particle Size	    23

2.  Benzo(a)pyrene Pyrosynthesis  	    99

3.  Pathways for the Pyrosynthesis of Benzo(a)pyrene  .   100

4.  Carcinogenic Polynuclear Aromatic Hydrocarbons
    Identified in Urban Air	   101

5.  Aza-Heterocyclics and Polynuclear Carbonyl
    Compounds Identified in Urban Air 	   102

6.  Reaction Pattern Showing the Products of
    Decomposition of Unsaturated Hydrocarbons 	   103

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                        LIST OF TABLES

 1.   Carcinogenicity and Anticarcinogenicity of Some
     Compounds Present in Polluted Air 	      7

 2.   Resource Costs Per Year of Diseases Associated
     With Air Pollution	     58

 3.   Summary of Recent Investigations in Spectral
     Methods	     69

 4.   Tumor Induction in Mice Following Cutaneous
     Administration of Organic Extracts of Particulate
     Atmospheric Pollutants  	    104

 5.   Tumor Incidence Following Injection of Organic
     Atmospheric Pollutants to Neonatal Mice 	    106

 6.   Estimated Annual Benzo(a)pyrene (BaP) Emissions For
     the United States	    107

 7.   Benzo(a)pyrene Concentrations in Urban Sampling
     Sites for January Through March 1959	    110

 8.   Polynuclear Hydrocarbon Content of Particulate
     Matter for Selected Cities  	    113

 9.   Seasonal Effect on the Benzo(a)pyrene
     Concentrations of Various Urban Atmospheres ....    114

10.   Concentrations of Benzo(a)pyrene in the Ambient
     Air, 1966	    115

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1.   INTRODUCTION

            According to some estimates,-1-2'108'1^8 the mortality

    rate from lung cancer has increased about 30 times since

    1900.  This alarming increase has occasioned studies concern-

    ing the cause of cancer.  A portion of the organic material

    present in the atmosphere, generally as suspended particulates,

    has been identified as carcinogenic to experimental animals.

    These organic carcinogens have been identified in the atmo-

    sphere of virtually all large cities in which surveys have

    been conducted.  It is true, however,  that in no case has a

    suspected organic carcinogen been demonstrated to produce

    lung cancer in humans.
                                i ftft
            According to Sawicki,   the composition of the urban

    atmosphere is as yet  (1967) undetermined, the vast majority

    of the constituents being unknown.  Certain organic compounds

    present as particulates in the atmosphere have been identified,

    and several of these have been found to be carcinogenic to

    animals.  There is also evidence that other organic compounds

    may be carcinogenic.

            Although there is some question as to the carcinogenicity

    of selected compounds  (such as those marked with an asterisk),

    the major classes of organic carcinogens are as follows:

            Polynuclear Aromatic Hydrocarbons  (PAH)
                     Benzo (a)pyrene (BaP)
                    *Benzo (e)pyrene
                     Benz(a)anthracene
                     Benz(e)acephenanthrylene
                     Benzo (b) f luoranthene

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                  Benzo(j)fluoranthene
                 *Chrysene
                  Dibenzo(e,1)pyrene
                  Dibenzo(a,h)pyrene
                  Indeno(l,2,3-cd)pyrene
                  1,2-Benzanthracene

         Polynuclear Aza-Heterocyclic Compounds
                  Dibenz(a,h)acrid ine
                  Dibenz(a,j)acridine

         Polynuclear Imino-Heterocyclic Compounds

         Polynuclear Carbonyl Compounds
                 *7H-Benz(de)anthracen-7-one

       *Alkylating Agents
                 Aliphatic and olefinic epoxides
                 Peroxides
                 Lactones

        The carcinogenicity of the above organic compounds

has been established through laboratory experimentation by

many investigators.  It should be noted that, in many ex-

periments, A-strain mice particularly susceptible to tumor

development were used, and only in two studies was inhalation

used as the route of administration.125

        The presence of substituent groups, particularly

alkyl groups, on some of these or other PAH compounds can

cause a profound change in their carcinogenic activity.1^8

Thus, while both benz(a)acridine and benz(c)acridine are

inactive when placed on the skin of mice, their  7-methyl

derivatives are very active  (rated similar to BaP in carcino-

genic activity).228  Some of the other methyl derivatives of
                                    228
these two compounds are also active.     Furthermore, whereas

chrysene was found inactive on the subcutaneous  tissue of

mice, the 4-, 5-, and 6-methyl derivatives were  all  active.

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        The major source of  atmospheric organic carcinogens



is the incomplete combustion of organic matter.9^  A secondary



source is the photochemical  decomposition of noncarcinogenic



organic compounds into more  active compounds.  Incomplete



combustion, which generates  organic carcinogens, occurs in



the process of heat generation, refuse burning, operation of



motor vehicles, and several  industrial processes.  Of the



compounds generated, the most extensively studied are the



polynuclear aromatic hydrocarbons  (PAH), particularly benzo(a)-



pyrene  (also known as 3,4-benzopyrene or BaP), which is con-



sidered the carcinogen most  active to animals.  Much of the



literature pertaining to the air pollution aspects of organic



carcinogens concentrates on  the compound BaP, due in part to



its high relative carcinogenicity, and also in part to the



fact that its identification and quantitative determination



in air are relatively simple.  Information concerning the



other known or suspected carcinogens is limited or nonexistent,



and in the words of Sawicki,-1-88 "We have no knowledge as to



whether the members of this great number of unknown trace



chemicals are innocuous, beneficial, or harmful to life in



the concentrations at which they are present in the various



nonurban,  industrial,  and urban atmospheres."



1.1  Types of Organic Carcinogens



1.1.1  Polynuclear Aromatic Hydrocarbons (PAH)



        The majority of polynuclear aromatic hydrocarbons



(PAH)  in our environment evolve from high-temperature

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                                                              4
reactions under pyrolytic conditions during  incomplete




combustion of organic matter.  The  formation of  PAH during



such processes occurs in two distinct types  of reactions,



pyrolysis and pyrosynthesis.  At temperatures above 400° to



500°C, organic components are partially cracked  into smaller,



unstable molecules  (pyrolysis).  These fragments, mostly



radicals, recombine  into larger, thermodynamically favored



and relatively stable PAH and heterocyclic hydrocarbons



 (pyrosynthesis).103



        Badger and his co-workers have studied the pyro-



synthesis of PAH.-1-0' I1  Their original working hypothesis is



illustrated in Figure 2 in the Appendix.  From these data



and from information acquired by other investigators,



the following concept has been formulated:   the  temperature



that exists during the burning of organic matter easily



breaks single carbon and carbon-hydrogen bonds to yield free



radicals.  These radicals combine and are dehydrogenated to



form aromatic ring systems that are relatively stable; only



the carbon-hydrogen  bonds of these components are broken to



any significant extent.  Long-chain paraffins, widely found



in fuels and plants, serve as special precursors of PAH.



Badger's original concept of PAH formation was verified by



extensive, carefully designed and carefully  executed experi-



ments utilizing l^C-labeled precursors.  Figure  3 in the



Appendix summarizes  the steps of BaP pyrosynthesis as they



are suggested from the experimental findings.  This concept

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can be extended to include hydrocarbons with uneven carbon

                                                        pc
numbers since methane also serves as a precursor of PAH.


        Pyrolysis experiments like these were conducted in


a nitrogen atmosphere and have been criticized for not


accurately reflecting the conditions that prevail during


the actual burning of organic matter.  As Hoffmann and


Wynder  3 point out, the fact remains that these studies


agree qualitatively with actual burning conditions of


organic matter, and the cited studies were conducted primarily


to discover the major precursors and pathways for the pyro-


synthesis of PAH rather than to compile quantitative data on


pyrosynthesis.


        In studies of actual burning of organic matter, a


reducing atmosphere exists around and/or inside the burning


cone, as well as inside the combustion chamber.  Newsome and


Keith-*--^ found this type of reducing atmosphere in the inner


cone of a cigarette, where it consists partially of hydrogen


(8.2 volume percent), carbon monoxide (11.8 percent), and


methane (1.3 percent), and contains only traces of oxygen


(1.4 percent).  Gasoline engine exhaust gases generated


during idling may contain up to 18 volume percent carbon


monoxide, 2 percent volatile hydrocarbons, and traces of


hydrogen.  The concentrations of carbon monoxide, methane,


and hydrogen and the absence or very low concentrations of


oxygen in the combustion products  indicate the existence of

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a reducing atmosphere around and/or inside the flame or



combustion chamber.  A reducing atmosphere is essential for



pyrosynthesis of PAH.



        As indicated in Figure 3 in the Appendix, other PAH



can be formed by the preceding mechanisms.  Long and his


          145
co-workers    at the University of Birmingham have also shown



that many PAH are formed during incomplete combustion of



ethylene and ethane.  The structures of several representa-



tive PAH which have been identified in urban air are shown



in Figure 4 in the Appendix.  Not all of the PAH, however,



are carcinogenic (see Table 1).



        In addition to the PAH in our environment that envolves



from incomplete combustion of organic matter, a small portion of



it may derive from thermic or catalytic cracking of organic



components.  The chemical mechanisms leading to the forma-



tion of trace amounts of PAH by cracking are similar to those



occurring during incomplete combustion.



        PAH occur in the atmosphere primarily as adsorbed



compounds on soot particles.  The biological effect of the



carcinogenic agents is critically related to the physical



characteristics of the particle.  Particle size primarily



determines the extent of penetration into the tracheobronchial



tree.119' ^   To a great extent, particle size also governs



the rate and extent of the elution of carcinogenic hydro-



carbons from the soot particles on which they are adsorbed.

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                         TABLE 1
                                                             7
     CARCINOGENICITY AND ANTICARCINOGENICITY OF SOME
                                          p o i I 7
	COMPOUNDS PRESENT IN POLLUTED AIR   	
                       Carcinogenicity    Anticarcinogenicity
	Compound	Ref. 117  Ref. 28	Ref. 28	
Benzanthracene           +         +               +

Benzo(e)pyrene           +         -               +

Benzo(a)pyrene          +++       +++

Anthanthrene             -         -               -

Perylene                 _         _               +

Benzo(g,h,i)perylene     -         -               -

Benzo(k)fluoranthene     -                         +

Benzo(b)fluoranthene    ++        ++

Benzo(j)fluoranthene    ++

Fluoranthene             -         -

Indeno(l,2,3-cd)pyrene   +

Anthracene                         -               +

Phenanthrene

Pyrene                   -         -               +

Benz(m,n,o)fluoranthene            -               +

Chrysene                 +         +               +

Benzo(c)acridine                   +               +

Benzo(a)fluorene                   -               +

Fluorene

Coronene                 -         -               -

Benz(a)carbazole                   +               +

2-Naphthol	-	-	
         Inactive:  -; weakly active:  +; moderately active:
++; strongly active:  +++.

         Carcinogenic properties may be due to the impurity
5H-benzo(b)carbazole present in "commercial pure" chrysene.

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                                                              8
See Section 2.1.2 for further discussion of particle size.



1.1.2  Polynuclear Heterocyclics and Oxygenated Compounds



        Large quantities of aza-heterocyclic compounds are



found in the compounds contained in some air pollution source



effluents, "' in very low concentrations in automotive ex-



haust fumes,    and in moderate concentrations in the air.214


       188
Sawicki    states that many of these compounds and some others



have never been tested for carcinogenic activity; dibenz(a,h)-



acridine and dibenz(a,i)acridine (see Figure 5 in the Appendix),



however, are carcinogenic.  Many polynuclear carbazoles have



been found to be carcinogenic. 4   In general, however, informa-



tion is scanty in this area.



        In regard to polynuclear carbonyl compounds, Hoffmann


          •I QO
and Wynder    note that, in theory, many known nonvolatile



"oxygenated substances" may exist in our respiratory environ-



ment.  However, very few chemical studies concerning these



compounds have been reported.  This is surprising, since



many neutral "oxygenated" compounds have been found in


              287
tobacco smoke.



        The paucity of chemical data in this area may be a



consequence of a lack of biological information on the sub-



fractions containing oxygenated substances.



1.1.3  Alkylating Agents



        Common alkylating agents found in the atmosphere



include epoxides, peroxides, and lactones.

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        Certain epoxides and peroxy compounds have been shown
                   1 9 2
to be carcinogenic,       '     as well as to have other

                      I I Q
physiological effects.     A recent source of these types of


compounds is the universal use of liquid fuels, such as


gasoline and diesel fuel, in the internal combustion engine.   '


In the unburned state, these liquid fuels are highly reactive


and when volatilized, undergo oxidation in the presence of


oxides of nitrogen in a photochemical reaction.  The reaction


products consist of a broad group of peroxides, including


compounds with epoxidic linkages.  The unburned gasoline and


other exhaust products emitted from automobiles contain


significant quantities of ethylehe, propylene, butene,  and


other unsaturated hydrocarbons.  Under certain meteorological


conditions, these vapors react in the presence of sunlight


and oxides of nitrogen to form a broad spectrum of hydro-



carbon oxidation products.


        Interest in compounds belonging to this group has


centered primarily around the formation of epoxides because


of the demonstrated carcinogenic potency of certain classes


of these compounds .


        Sato and Cvetanovic    studied the decomposition of


unsaturated hydrocarbons; the products of decomposition



following photochemical reaction include compounds with


epoxidic linkages.  A typical reaction is shown in Figure 6



in the Appendix.  These reactions account for the major de-


composition products, which include formaldehyde and acetone

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                                                              10
as well as epoxides.  Some of the latter compounds belong to



the group of experimentally verified carcinogens.   Apparently,



significant quantities of epoxides are produced.



        The concentration of hydrocarbons in the air from



auto exhaust is about 3.4 ppm.     Of this amount, 1 ppm is



made up of saturated hydrocarbons, and the remainder consists



of olefins associated with 0.05 ppm diolefins.  Ethylene,



constituting half of the olefins produced, is of special



significance.  When the automotive exhaust is exposed to



light and air.- photochemical reactions occur which destroy



56 percent of the total amount of hydrocarbons in a 2-hour



interval.  Two-thirds of the hydrocarbons which are destroyed



are olefinic (excluding ethylene), i.e., olefins which are



known to give a 17 percent epoxide yield.  Thus, 6 percent



of the total hydrocarbons emitted from the exhaust are trans-



formed to epoxides of the type that have been found to be


                                     28
carcinogenic to experimental animals.    The controlled



reaction is equivalent to 2 hours of exposure to photochemical



reactions that occur naturally in the Los Angeles atmosphere.



The epoxide concentrations determined are conservative.119



1.2  Organic Carcinogens as Cocarcinogens or Anticarcinogens



        Little information is available regarding the syner-



gistic or antagonistic effects of organic carcinogens.  How-



ever,  the limited animal studies available have demonstrated



that organic carcinogens do play a role as cocarcinogenic

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                                                             11
or anticarcinogenic agents.  There are data that suggest



that phenols and some long-chained hydrocarbons are co-



carcinogenic.   '



        Table 1 in Section 1.1.1 shows some PAH that were



reported by Kotin and Falk121 to have anticarcinogenic



activity.  These authors also presented data that demonstrated



that the carcinogenic properties of dibenz(a,h)anthracene, a



strong carcinogen, can be strongly inhibited by the dihydro-



and the hexahydro-derivatives of dibenz(a,h)anthracene, as well



as with benz(a)anthracene and phenanthrene.  Several other



authors have reported that a weak carcinogen or noncarcinogen



can inhibit the activity of normally potent chemically



related carcinogens.40'126'132'237

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                                                                  12
2.   EFFECTS



            According to Pybus,    cancer has puzzled mankind



    since it was first recognized in the fourth century B.C.



    The first and most important observation of its cause was



    made by a London surgeon, Percival Pott, who in 1775  des-



    cribed cancer of the scrotum in chimney sweeps; he decided it



    resulted from the soot lodged in the crevices of the skin



    of the male genitals.     This apparent causal relationship



    between soot  and skin cancer led to attempts to produce



    cancer in animals by painting tar on their skin.  The



    earliest experiments, performed about 1889, failed because



    they were conducted on resistant animals or because not



    enough time was allowed for the cancer to develop.  Thus, the



    demonstration that soot or tar was actually responsible for



    this form of cancer remained unproved until 1915, when skin



    cancer was produced in a rabbit by means of repeated applica-



    tions of extracts of tar.16^



            After World War I, British investigators, under the



    leadership of Sir Ernest Kennaway, demonstrated by persistent



    and tedious experimentation that skin cancer could be induced



    in mice by coal tar.  Fractionation of the tar to isolate the



    active ingredients led to the discovery that the tumor-produc-



    ing constituents were also highly fluorescent.  Thus, examina-



    tion of fractions for fluorescence guided the separations



    rather than lengthy animal tests, and rapid progress was

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                                                         13
made.  A discovery followed shortly:  certain PAH, such as



the now familiar BaP contained in the tar are responsible in



part for the tar's carcinogenic effect.



        Numerous studies have demonstrated a worldwide



increase in the frequency of and mortality from lung cancer.



These investigations leave little doubt that the apparent



increased incidence of lung cancer is both real and, at least



until very recently, progressive.  Interest in this lung



cancer increase has sparked many biological and medical



investigations.  Some of the studies have been concerned with



the effect of air pollution and/or smoking.  Many of the stud-



ies relating air pollution to cancer in humans are epidemio-



logical.  As already noted, airborne carcinogens have been



the subject of considerable study in the laboratory.



       Although cigarette smoking has been a widely publi-



cized issue for more than a decade, it is generally agreed,



however, that a history of cigarette smoking is causally



related to the risk of developing lung cancer.  Any meaningful



discussion of the relation of lung cancer to cigarette smoking



is clearly beyond the scope of a survey directed toward



national air pollution control.  A review of this subject by



Rigdon and Kirchoff    covers the years 1930 through 1960.



More recent articles have dealt with the chemical composition


                 235 288
of tobacco smoke,   '    the mechanism of carcinogenic action

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                                                         14
                 *7 1                         O O £\
of tobacco smoke,   smoking and oral cancer,    smoking and
                   174
cancer of the lung,    smoking and cancer of the urinary
tract,   and retention of cigarette smoke by the lungs.43
In addition, the U.S. Public Health Service has issued over-
                                95 232
all views of smoking and health.  '
                                        {
       A related area which is pertinent to this survey is
the impact of smoking as compared with air pollution on
development of lung cancer.  The reports seem to indicate
that the joint effect of cigarette smoking and air pollution
is additive rather than multiplicative; however, the evidence
is still inconclusive.23'24'247'285

2.1  Effects on Humans
       Although no suspected organic carcinogen has been
                                                     O
proved experimentally to cause lung cancer in humans,
an apparent association has been shown between air pollution
and human mortality rates from lung cancer.  Furthermore,
organic carcinogens have been shown to increase tumor
activity in experimental animals.  Data on experimental
animals is presented in Section 2.2.

       The increase in the absolute mortality resulting from
cancer (especially of the lung) has been demonstrated in vari-
ous parts of the world during the past half century.  Kotin
        119
and Falk    indicated that this increased mortality has been

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                                                              15
characterized by the following: (1) greater susceptibility to



lung cancer in urban than in rural residents; (2) greater



incidence among individuals with a history of prolonged and



excessive cigarette smoking; and (3) greater frequency of lung



cancer in males than in females.



        The increased incidence of lung cancer parallels the



introduction of new etiological agents into the respiratory



environment around the turn of the century.     Since lung



cancer, as other neoplasms, is not likely to result from a



single initiating or promoting experience, it is important to



evaluate the pathogenic significance of polluted air.  This



evaluation is necessary both for the detection of carcinogens



and the determination of the role of air pollution in relation


                               119
to other environmental factors.


                      ......    37,42,49,51,60,61,88,102,119,
        Numerous investigations


128,162,222,242,285,289 ,     .  ,.   .  ,   , . ,       .,      _
   '                     have indicated a higher incidence of



lung cancer for urban residents than for rural residents.


                                       55,56,87,88,153,219,284
Studies relating smoking to lung cancer



also show the so-called "urban factor."  After correcting for



the differences in smoking habits between urban and rural



residents in the United States, the rate of lung cancer was


                                      88
still 25 percent lower in rural areas.

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                                                              16
        The  nature  and  significance  of  the  urban  effect have




not been  specifically established.   It  may  result  from occu-




pational  differences,  from rural  patients'  habit  of obtaining




improved  medical  care in the  city and reporting as urban resi-




dents,  from  improved  accuracy of  cancer reporting in  urban




areas,  or from a  combination  of these and other factors.


                               22
        In 1964 Buck  and Wicken    reported  an  association



between the  degree  of air pollution  and the incidence of




lung cancer  and bronchitis mortality in two areas  of  the Eston


                                                   294

(England) urban district.   However,  Zeidberg .et. aJ._.    reported



(also  in  1964) on a study of  9,313 individuals in  Nashville,



Tenn.,  in which they  found no evidence  of a relationship




between air  pollution and  cancer  morbidity  rates.




        Nevertheless,  exposure to polluted  air is  a widely accepted



explanation  for the consistent observation  of  increased lung




cancer  incidence  in urban populations.   Epidemiological,




physicochemical,  and  biological data are consistent with an




etiological  role  for  polluted atmosphere.


                       121
        Kotin  and Falk     cite the following evidence to support



the contention that air pollution may be a  significant factor




in the  pathogenesis of  lung cancer:




        (1)  Carcinogenic agents have been identified  and




quantified in  the polluted air of essentially  every city in



which they have been sought;

-------
                                                             17
        (2) Similarly, chemical compounds with known tumor-



promoting properties have been identified and quantified in



polluted urban air;



        (3) The stability and survival of carcinogenic hydro-



carbons in the atmosphere are compatible with inhalation and



a postulated biological effect in those exposed;



        (4) Carcinogenic agents, as well as noncarcinogenic



respiratory epithelial irritants, occur in the atmosphere in



a state compatible with host entry and tracheobronchial



deposition;



        (5) Alteration in function and structure of the respir-



atory epithelium of representative mammalian species has been



demonstrated following exposure to a broad spectrum of these



environmental irritants.  The resulting changes appear to



facilitate the biological action of carcinogenic agents; and,



        (6) Bioassay by skin-painting and subcutaneous-injection



techniques has established the carcinogenic properties of com-



pounds identified in and extracted from polluted air.  Exposure



of both susceptible and resistant strains of mice to aerosols



of synthetically reproduced polluted urban air has resulted



in lung tumors in both strains.


                                 ^ .   .     ^ 5,49-51,61,62,85,
        Epidemiological studies of immigrants



   '     indicate that air pollution may be an etiological factor

-------
                                                              18
which has long-term residual effects with a latent period of




induction.  British immigrants to South Africa had a higher




incidence of mortality from lung cancer than the native white




South Africans, who smoked even more than the immigrants.  '




This was observed in British immigrants who had resided in




South Africa even for 20 or more years.  Similar studies indi-




cate that immigrants to New Zealand,   '   Austria,   Israel,



          148
and Mexico    showed a greater pattern of lung cancer than was




found in their new area of residence.  In contrast, Norwegian




immigrants who moved to the United States, where the air




pollution was greater than in their home country, had a cancer




rate higher than native Norwegians but less than the native



          85                                                   86
Americans.    It has been further observed in the United States




that rural residents who previously resided in an urban area




for 10 years or more had a greater risk of lung cancer, inde-




pendent of the smoking history than the corresponding rural




residents who had no urban exposure.




        In a recent report based on epidemiological findings,




Stocks    hypothesized that air pollution accelerates the




final stages of cancer growth in susceptible persons who have




reached an advanced point in the latent interval of the




disease.

-------
                                                              19
        Attempts have been made to correlate the amount of



atmospheric BaP pollution with the development of lung cancer.


                   248
Stocks and Campbell    observed a higher incidence of lung



cancer among persons living in an area in which BaP was known


                                         221 222        244245
to be present in the atmosphere.  Shahab,   '    Stocks,


           83
and Gorman    observed a relationship between the increased



mortality from lung cancer and the concentration of carcino-



genic PAH in urban areas.


                                   188,217
        In contrast, Sawicki et al.        in several studies



have not been able to demonstrate a correlation between lung


                                                              188
cancer death rates and the amount of atmospheric BaP-  Sawicki



suggests that because airborne carcinogens are so numerous,



many have not yet been identified.  He further states, "The



number and type of carcinogens found in the atmosphere indicate



that attempts to correlate carcinogenicity of the mixtures in



our chemical environment with concentrations of BaP are probably



naive in most cases and spring from our lack of knowledge of



the composition of the mixtures with which we are dealing."


             109
Hueper et al.    were unable to find a correlation in a com-



bined analytical and experimental bioassay study using samples



of polluted air from eight metropolitan centers in the United



States.



        It is now generally accepted that usually many factors

-------
                                                              20
 are  involved  in the  initiation  and  promotion  of  cancer.




 Consequently,  a correlation between the  incidence  of  cancer




 and  any  single suspected  environmental factor is extremely




 difficult  to  establish as well  as quite  limited  in usefulness.




 2.1.1  Particulate Matter




         The occurrence of gastric cancer has  been  linked with




 the  presence  of suspended particulate matter  in the atmosphere.




 Anderson and  Coffey  hypothesized that the causative  agent of




 gastric  cancer is associated with soot,  winkelstein and



      43
Kantor   have  found that  mortality rates from gastric cancer



 in a selected  population  were twice as high in areas of high



suspended particulate  air  pollution as in areas of  low pollu-



tion in  a northeastern United States industrial area.  The



exact nature of the particulate was not  specified;  however/



 in areas of high industrial air pollution, soot is  usually


                                                 295 296
a significant  constituent.  In a Nashville study,   '



soiling  indices were found to correlate  directly with mor-



tality from stomach cancer and with prevalence of bladder,



esophagus, and prostate cancers.  No clear correlation was



 found between  soiling  indices and mortality from lung,



bronchial, or  large intestinal cancer.


                              144
      In 1963,  Litvinov et al.    studied the relationship



between  pulmonary cancer  in man and air  pollution resulting



from the discharge of  carcinogens by aluminum plants.  Over



10 kg of BaP were discharged into the air daily resulting in

-------
                                                          21
the deposit of an average of 9.1 ug per square mile per day



of this material on the ground in the area of the plant.



These high concentrations of BaP were implicated as a cause



of chronic morbidity of the respiratory tract.





        Konstantinova and Chertova    studied the effect of



dust and other airborne contaminants on lung cancer morbidity.



Two population districts of Ufa in Russia were studied.  The



first area was located between two petroleum refineries and



the other was situated 20 kilometers from the refineries.  The



symptoms characteristic of chronic hydrocarbon poisoning were



observed more than three times as often in the population near



the refineries than in the one 20 kilometers away.  The lung



cancer mortality rates per 10,000 population were 0.8 for the



noncontaminated area and 1.28 for the contaminated area.



        The carcinogenic properties of soot are believed to


                                                 248 271
result from the presence of certain adsorbed PAH.   '


              120
Kotin and Falk    have studied the role of soot in lung cancer,



Their findings indicate that soot is a powerful carcinogenic



agent because of its ability to adsorb large quantities of



known carcinogens.  In addition, certain physical properties



of the soot, such as particle size, facilitate transport into



the lungs and elution of these carcinogens by body fluids.

-------
                                                             22
2.1.2  Particle Size




        PAH and other organic carcinogens are primarily pres-



ent in the atmosphere as adsorbed compounds on soot parti-



cles.     The biological effect of the organic carcinogens is



critically related to the physical properties of the particle



on which it is adsorbed.  Particle size largely determines the



extent to which particles can penetrate the tracheobronchial



tree. 2   Particles in the size range of 125 R to 2.5 |a are



of great biological importance.  Particles larger than 2.5 p.



gaining host entry are largely retained in the mucous membranes



of the nose, oral cavity, pharynx, and nasal sinuses.  Parti-



cles less than 125 A remain largely suspended in the circu-



lated air, and thus pulmonary retention of these particles is



rather small.  The pulmonary retention of particulate matter



by the lung in relation to particle size has been given by


                  47
Dautrebande et al.    Figure 1 shows the relationship between



retention of particulate matter and particle size.



        The size of the particulates on which the carcinogenic



hydrocarbons are adsorbed also determines the rate and elution


                                       70          236
of the hydrocarbons from the particles.    Steiner,    using



commercial carbon blacks, was able to relate the skin carcino-



genic activity of adsorbed carcinogens to the size of the soot



particle and to the presence of natural eluting substances in

-------
                                                                    23
Retention

   80


   70


   60


   50


   40


   30


   20


   10
       .25 .50 .75 1.0


2.0       3.0        4.0

    Particle size (microns)
                                                      5.0
                             FIGURE 1

             Retention of  Particulate  Matter in
             Lung in  Relation to Particle  Size47

-------
                                                             24
the skin at the point of deposition.  He concluded, "The


principles of natural and conditioned carcinogens, of solvent


elution, and of adsorption are advanced to explain some clini-


cal and epidemiological observations on human skin and lung


cancers, and on the role of preceding pathological lesions in


predisposing to pulmonary tumors."  It has been suggested that


the solvent action of lipids and sebaceous secretions on the


skin exerts an eluting effect by liberating the carcinogenic


PAH from the soot particle.


        In chemical studies of soot recovered from human lungs


during autopsy, Falk et al.   could not demonstrate the presence


of BaP, although traces of pyrene were detected.  It was possi-


ble, however, to demonstrate experimentally the entry of soot


particles of appropriate size into the cells of the respira-


tory tract of mice, rats, and rabbits, both by histological


studies and by the fluorescence of BaP (using fluorescence

                       120
microscopy techniques).     It then became necessary to deter-


mine the effect of cellular and plasma proteins on the elution


of PAH from soot.  It was found that PAH is readily eluted


from commercially prepared soot particles of 0.5 microns.


When the particle size population was mixed, the liberated


PAH from the larger particles was adsorbed by the smaller

                                             o
particles.  Thus, particles of less than 800 A in diameter

-------
                                                             25
do not yield PAH in the presence of physiological eluting




agents.  Instead, they serve as scavengers for the readsorption




of the PAH eluted from larger particles.




2.1.3  Irritants




        In addition to the normally recognized carcinogens,




other organic compounds usually present in polluted urban air




may act as irritants to the respiratory system, triggering




physiological responses.  These responses may result in the




formation of malignant tumors or render the system more sus-




ceptible to the usual pathogenic effects of substances generally




recognized as carcinogens.  Irritants in the respiratory envi-




ronment can interfere with ciliary activity and the flow of




the mucous stream in such a way that particulate matter accumu-




lates on the underlying cells.  The typical response of ciliated




mucous-secreting epithelium to irritant aerosols is character-




ized by a small increase in the rate of flow, followed by a




slower flow rate that eventually returns to normal.  The bio-




logical effect of the alterations in the rate of mucous flow




is one of increased particle retention, enhancing the oppor-




tunity for tumor formation.  This phenomenon represents a




step in the pathogenetic sequence in which compounds that are




not in themselves carcinogenic appear to facilitate the bio-




logical activity of compounds capable of inducing cancer in

-------
                                                          26
                      119
the respiratory tract.


        Among the compounds normally present in polluted


urban air implicated as irritants are:  formaldehyde, acet-


aldehyde, acrolein, propionaldehyde, formic acid, acetyl


peroxide, peracetic acid, propylene oxide, and cyclohexene


oxide.68



2.1.4  gtudies with Biological Material


         Cultures  of human fetal  lung tissue were  exposed  to

                  41
BaP  by Lasnitzki,   and  to fractions of cigarette smoke con-

                                  136                        135
densate  by  Lasnitzki  and  Kennaway   and  by Lasnitzki alone.


Basal cell  hyperplasia and  metaplasia  of  the epithelium,  pro-


liferation  of bronchial  units, and  suppression of mesenchymal


elements  occurred during 2  or more weeks  of cultivation under


continuous  exposure to BaP.  A hydrocarbon-free fraction  of


cigarette smoke condensate was also active  (producing squamous


metaplasia), indicating  that the effects  of the condensate


could not be attributed  to hydrocarbons alone.

                      179
         Rounds et al.     demonstrated  that an established line


of human  conjunctival cells show an increased growth rate and


undergo  increased chromosomal aberrations when treated with


chloroform  extracts of automobile exhaust.  He also showed


that 3-methyl-4-dimethyl-amino-azobenzene induced the following



changes  in  human conjunctival cells in a  suitable growth

-------
                                                              27
medium:   growth  stimulation,  increased  chromosomal  stickiness
and  scattering during  mitosis,  and  a  decrease  in  a  positive
                                    178
staining  reaction  for  phospholipids.
        Allison  and Mallucci  demonstrated that the carcinogens
BaP  and 9,10-dimethyl-l,2-benzanthracene  (DMBA) are concentrated
by the lysosomes.  The genetic  perpetuation of carcinogen-induced
metabolic changes  has  been  explained  on the basis of specific
                                             ICO
disruptions  in metabolic  regulatory circuits.     Experiments
which purported  to show an  intercalation  (mutagenic interaction)
between carcinogens and deoxyribonucleic  acid  (DNA) have been
                          7R
criticized by Giovanella. °
                   106
        Hsu  _et al.     described the inhibitory effect of certain
carcinogenic aromatic  hydrocarbons  on the replication of single
stranded  ribonucleic acid (RNA) and DNA.  Hsu et al.    later
demonstrated the inhibition of  viral  nucleic acid and protein
synthesis  in Escherichia  coli by DMBA.
2.2  Effects on Animals
2.2.. 1   Commercial and Domestic Animals
        No information is available concerning the  effects of
organic carcinogens on commercial and domestic animals.  How-
ever, Pybus    reports that virtually every form of benign or
malignant tumor has been  found  among  domestic animals.  Cancers
of the mouth, throat,  breast, and other body parts are

-------
                                                              28
relatively common among older cats and dogs.


               240
        Stewart    examined 36 wild mammals and birds from



the Philadelphia Zoo for pulmonary cancer, pulmonary adeno-



matosis, and squamous metaplasia.  Histologic material



available on 31 of the animals indicated: adenocarcinomas



in 11 birds of the family Anatidae, a silver pheasant, a



red jungle fowl, and a coypu; squamous cell carcinoma in a



North American otter; undifferentiated small-cell carcinoma



in a Java sparrow; alveologenic carcinoma in a striped skunk;



pleural mesotheliomas in a Cape hunting dog and a clouded



leopard; adenomatosis, squamous metaplasia, or both in a red



fox, puma, North American otter (which also had squamous cell



carcinoma of the lung), squirrel monkey, peafowl, African



eared vulture, Chinese myna, 2 toucan barbets, and a



rabbit-eared bandicoot; tuberculosis in a civet; pneumonia



in a kangaroo; and metastatic renal carcinoma of the lung in



a red wolf.


2.2.2   Experimental Animals



        The direct effect on animals of organic carcinogens,



normally present in polluted urban air, is difficult to



evaluate.  Most of the information available on the effect of



organic carcinogens on animals has been obtained under experi-



mental conditions, which are different from the conditions

-------
                                                              29
which normally constitute the natural environment of the




animal.




        There has been extensive investigation of the effects




on experimental animals of specific compounds that pollute




the atmosphere.  Much of the work has utilized skin painting


                               97            28
with extracts of chimney soots,   road dusts,   and vehicular




exhausts,104'124 or injections of these extracts.109'125'140'141




In general, inhalation studies have not attained the same




degree of success as those using painting and injection




techniques.  The induction of bronchogenic carcinomas in




experimental animals has often required such drastic treat-




ments as transfixion of the lungs with carcinogen-impregnated




threads,    intrabronchial implantation of wire pellets




impregnated with carcinogens,    and the use of radioactive



          134
materials.




        In recent years, successful production of experimental




lung cancer has been reported through use of the following




test compounds or mixtures? "artificial smog" (ozone-treated


                1 O C 1 "7 O                          1 ft O 1 Q *3

gasoline vapor),   '    BaP adsorbed on hematite,   '    BaP




in polysorbate 80,    an aerosol containing both ozonized-gaso-



                         123 281
line and influenza virus,   '    and mixtures of phenolic and


                                  O £\ "3

nonphenolic extracts of coal tars.     The investigations of




Kotin and his co-workers,   '   '    and of Tye and Stemmer,

-------
                                                              30
employed natural inhalation of the "polluted air," whereas



the other experiments used intratracheal instillation.



        For test animals, investigators have often used a



strain of rodents that is genetically susceptible to "spon-



taneous" formation of the type or types of cancer being



studied.




        The carcinogenicity of 14 mono-, di~, and trimethyl-



ated  BaP was studied by Lacassagne et_ aJL_.    using labora-



tory mice as test animals (three injections of 600 |jg each).



The majority of these compounds showed highly pronounced



sarcomagenic properties, both regarding the percentage (almost



100 percent) of animals developing tumors and the latency



period (100-150 days).  Many of these homologues were more



active than nonsubstituted BaP.  From this study, the authors



concluded that introduction of more than three methyl groups



into the BaP molecule would lead to a loss of carcinogenicity.


                            250
        Stokinger and Coffin    have discussed the importance



of the enhancement of organic carcinogen action  (such as that



of BaP) with seemingly inert particles.  Iron oxide in par-



ticular appears to have properties that possibly contribute



to cancer production.  These authors cite the work of Saffiotti


      180 181 184
et al.,  '    '    who produced a variety of malignant tumors

-------
                                                             31
in the lungs of hamsters.  The animals were intratracheally

injected with a saline suspension of BaP ground together with

hematite (Fe20a)  as a carrier dust in amounts equivalent to

3,000 ng of each chemical (a very high dose).  Fifteen weekly

injections were given.  Two important facts were revealed in

this study: a high incidence  of lung cancer was produced,

up to 100 percent of the animals in some experiments; and

these lung cancers mimicked all the cell types seen in human

lung cancers, i.e., squamous cell carcinoma, anaplastic

carcinoma, adenocarcinoma, and even tracheal cancers.  Dose-

response effects were indicated, as was the possibility that

a single high dose could induce cancers in the system.  Ac-
                           181
cording to Saffiotti et al.    the increased carcinogenic

action is due to the iron oxide, which penetrates the bron-

chial and alveolar walls into the lung tissue without extensive

damage or destruction of the ciliary and mucous barrier; the

iron oxide also acts as a vehicle to transport the carcinogen
airon oxide was chosen as the carrier dust because it has no
extremely irritating or toxic effects.  Saffiotti believes
that in order to carry the carcinogen in high concentrations
to healthy cells, an inert carrier is required—that is, a
carrier which does not destroy the cells.

 Benzo(a)pyrene alone has induced cancer in the lungs of exper-
imental animals, but usually with some difficulty and in low
yield.

-------
                                                              32
to the lung tissues.  The carcinogen is then eluted from the




particulates and spreads diffusely throughout the tissue.




They surmise that the rate of removal of BaP from the respira-




tory tract is slowed by action of the inert dust as it is




stored in macrophages.  Thus, the carcinogen remains in the



lung, unmetabolized in high local concentrations — an important




factor in producing cancer with BaP.  They also suggest that



such a mechanism is a realistic concept of what actually




occurs in nature as man breathes the polluted air—i.e.,



carcinogens adsorb on iron oxide or other "inert" particles



which act as a vehicle to transport the carcinogens into the




lungs through the respiratory tract lining to the lung tissues,



where the carcinogens are eluted by the cell plasma.  However,



it is yet to be shown that BaP is adsorbed onto iron oxide




particles in the atmosphere.



                       172
        Rigdon and Neal    studied the absorption and excre-




tion of BaP using ducks, chickens, mice, and dogs as test



animals.  There was no apparent acute injury when large amounts



(e.g., 250,000 |ag for ducks) of BaP crystals and/or BaP sus-




pended in a physiologic solution of sodium chloride, with a




1 percent solution of polysorbate 80 were given orally to




ducks, chickens, mice, and dogs and intratracheally to ducks.




BaP was detected in the blood of ducks as well as in the blood

-------
                                                              33
and bile of chickens and dogs.  The presence of BaP was also



detected in the skin of the chickens and mice.  The mesenteric



tissue, the gallbladder and kidneys, and the urine of the



treated mice, as well as the kidneys of treated chickens and



ducks contained BaP.


                       173
        Rigdon and Neal    found that although white Swiss



mice  (59 or more per group) fed commercial laboratory pellets



of BaP rarely had spontaneously occurring tumors in the stom-



ach,  they did have spontaneously occurring pulmonary adenomas.



BaP crystals were added to the commercial ration in concen-



trations of 250 and 1,000 |J.g per gram of food and fed routinely



to the mice.  Papillomas and squamous cell carcinomas in the



first portion of the stomach as well as an increase in the



number of pulmonary adenomas resulted.  All the mice fed the



1,000 M-g BaP-containing ration for 86 days or longer developed



gastric neoplasms.  When mice born of mothers fed BaP during



pregnancy and lactation were fed the control ration after



weaning, gastric tumors did not develop; however, littermates



fed BaP did develop such tumors.


                                154
        These same investigators    studied the growth of



young mice fed BaP-  They found that the rate of increase in



weight of litters of young mice nursed by mothers fed BaP



was lower than that of litters from lactating mothers fed a

-------
                                                              34
normal ration.  In some mice in the BaP-fed litter there was


a decrease in weight that began at 10 to 12 days of age.  After


the young mice were weaned and fed a standard ration, their


weight still remained lower than normal.  This diminution in


weight was attributed to a decrease in nutrition and not to a


toxic effect resulting from the BaP-

                       o a. T
        Toth and Shubik    investigated carcinogenesis in AKR


mice injected subcutaneously with BaP and dimethylnitrosoamine


(DMN).  In contrast to controls, mice injected with BaP pro-


duced malignant lymphomas more rapidly; and they developed


lung adenomas.  The DMN had no apparent effect on the develop-


ment of virally mediated lymphomas, but did cause both benign


and malignant liver tumors and lung adenomas.


        Pylev    investigated the effect of the dispersion of


soot on the deposition of BaP in the lung tissues of rats.


Two contrasting kinds of carbon black (soot) were administered


intratracheally: 5,000 |-ig channel black, with particles of 0.01 M-;


and 5,000 |j.g thermal decomposition black, with particles of


0.3 p..  He found that the coarsely dispersed decomposition


black promoted rapid disappearance of BaP from lung tissue,


whereas the finely dispersed channel black impeded the elimi-


nation of BaP and facilitated its decomposition.


        Shahab .et a^.225 studied the importance of local


retention of carcinogenic agents in the pathogenesis of lung

-------
                                                              35
cancer in rats.  In this experiment 280 animals were given




5,000 [ig BaP in suspensions of india ink in three doses by




intratracheal intubation.  It was found that BaP retention




was a function of the number of administrations of BaP, as




the number of exposures to BaP increased, the retention in




the lung was found to increase.


                1 a. C\

        Tomingas    found that the subcutaneous implantation




of starch-encapsulated soot on the back of rats resulted in



                                                          99
the formation of sarcoma in low yield.  Herrold and Dunham




showed that repeated intratracheal instillations of BaP sus-




pended in polyoxyethylene sorbitan monostearate (Tween 60)




induced papillomas and carcinomas of the trachea, main-stem




bronchi, and bronchioles in Syrian hamsters.  The mucosa of




the tracheobronchial tree showed both regenerative and atypical




epithelial changes.  Neoplastic lesions were not induced in




another group of animals that received intratracheal instil-



                                              227
lations of BaP dissolved in olive oil.  Shubik    reported




that bronchogenic carcinoma could be induced in hamsters by




using a colloidal suspension of 7,12-dimethylbenz(a)anthracene




administered by endotracheal intubation, but found that repeti-




tion of the study with BaP yielded uniformly negative results.


               QO
        Herrold   studied the effects of BaP, cigarette smoke




condensate, and atmospheric pollutants on the respiratory

-------
                                                              36
system of Syrian hamsters.  He observed that benign and




malignant tumors of the trachea, bronchi, and bronchioles




were induced following intratracheal instillation of BaP




suspended in Tween 60.  Only atypical epithelial changes




suggestive of precancerous lesions were observed with BaP




suspended in distilled water.  No significant changes were




noted in the animals that received intratracheal instillation




of BaP dissolved in olive oil.  These findings suggest that the




vehicle for the carcinogen as well as the physical state of




the carcinogen are important factors in carcinogenesis.




        The intratracheal instillation of cigarette smoke con-




densate and atmospheric pollutants in the hamsters produced




regenerative epithelial changes of the tracheobronchial mucosa.




These lesions included basal cell hyperplasia and peribronchial




and peribronchiolar proliferation.  Atmospheric pollutants




induced extensive adenomatous proliferation involving large




areas of the lung in all of the test animals.  No tumors of




the respiratory tract were induced either with cigarette smoke




condensate or atmospheric pollutants.




        Bogacz and Koprowski   investigated the comparative




carcinogenic properties of air pollutants, tobacco tar, and




BaP by means of correlated cytopathologic studies, utilizing




the uterine cervix of mice as a target organ.  It was

-------
                                                             37
demonstrated that mice of the ZBG and C3H strains treated



intravaginally with air pollutants and tobacco tar developed



cellular abnormalities and histologic lesions that were



morphologically indistinguishable from those accompanying the



development of BaP-induced carcinoma.



        The carcinogenicity of atmospheric pollutants in mice



has been demonstrated with organic extracts collected from



various sources, using various techniques (Table 4 in the



Appendix).  When pollutant extracts are administered to mice,



whether by painting or by subcutaneous injection, local tumors,



papillomas, carcinomas, or sarcomas—sometimes accompanied by



multiple pulmonary adenomas—have generally resulted.  Notable



exceptions were: (1) the high incidence of distant tumors,



hepatomas, and lymphomas, in addition to multiple pulmonary



adenomas; and (2) the virtual absence of local tumors following



subcutaneous injection of relatively small concentrations of



organic pollutant extracts in infant mice (Table 4 and 5 in



the Append ix).


                        I OO
        Saffiotti et al_.  ° reported that vitamin A prevented



development of tumors in hamsters.  Syrian golden hamsters of



both sexes received 10 intratracheal instillations each of



BaP and hematite suspended in saline.  One group received no



further treatment and developed 13 squamous tumors and 13 cases

-------
                                                              38
of squamous metaplasia in 53 exposed animals.  One week after


the end of the BaP treatment, the second group began receiving


stomach tube feeding of vitamin A palmitate twice weekly (con-


tinued for life).  In this group of 46 animals, only one de-


veloped a microscopic squamous tumor in the bronchus, while an-


other developed  a patch of squamous metaplasia in the trachea.  The


incidence of forestomach papillomas was also reduced markedly


by vitamin A treatment.  The authors suggested that vitamin A


has a systemic inhibitory effect on the induction of squamous


changes (metaplasia as well as benign and malignant squamous


tumors) in the columnar mucous epithelium of the respiratory


tract.


        In studying the influence of phenols in the production

                              O ^ O
of carcinomas, Tye and Stemmer    exposed five groups of mice


(20 per group) to aerosols containing blends (120 lag/liter)


of coal tar extracts, including phenols.  The mice were exposed


2 hours, three times weekly, for 55 weeks.  Animals were sacri-


ficed at various intervals.  The lungs and tracheas of all mice


were then examined grossly and microscopically for neoplasms


or relevant morphologic changes.  After 46 weeks, among 32


survivors in two groups, which received similar aerosols con-


taining phenols, there were 4 cases of adenocarcinoma, 19 of


intrabronchial adenoma, and 10 of squamous metaplasia.  In

-------
                                                              39
20 survivors of another group, which received the same tar




without phenols, there was no incidence of adenocarcinoma;




11 had intrabronchial adenoma and 2 had squamous metaplasia.




        Gardner   reported that laboratory mice exposed to




community photochemical smog levels for a 16-month period




showed a slight but statistically insignificant increase in lung




tumor development (adenoma) in ,the aging animals, when compared




with control animals that were exposed to filtered air.




2.2.2.1  Effects on Animal Tissue



                 41
        Lasnitzki   studied the effect of the carcinogenic




hydrocarbons 9,10-dimethyl-l,2-benzanthracene (DMBA), BaP, and




methylcholanthrene (MC) on organ cultures of suckling rat




trachea.  It was found that these hydrocarbons caused loss




of differentiated tracheal epithelial cells and of mesen-




chymal elements (including chondrocytes), but not of basal




cells.  The mitotic rate and the proportion of cells incorpo-




rating H -thymidine increased in response to hydrocarbons, but




the increase in H -thymidine uptake and mitotic rates was not




always proportionate, nor were these responses alike for all




hydrocarbons.




        The effects of DMBA, BaP, and MC were similar in that




all produced clear-cut increases in the proportion of basal




cells undergoing DNA synthesis.

-------
                                                              40
        In studying the effect of MC on prostate glands of


                      41
mature mice, Lasnitzki   observed that hyperplasia resulted



during exposure to 2 |ag/ml and 4 |-Lg/ml for 10 days; this



process continued after withdrawal and was accompanied by



squamous metaplasia.  Mitosis was increased 4 days after



exposure to MC.



        Mitosis and DNA synthesis in mouse prostate gland



tissue were compared by Lasnitzki and Pelc.     They con-



cluded that mitosis was stimulated before DNA synthesis.



2.3  Effects on Plants



        No information on the effects of organic carcinogens



on plants has been found in the literature.



2.4  Effects on Materials



        No information has been found indicating that materials



of commercial or industrial importance are affected by organic



carcinogens.



2.5  Environmental Air Standards



        Recommended limits of 200 |ag/m3 for an 8-hour workday



have been adopted by the American Conference of Governmental


                     o CQ
Industrial Hygienists    for coal tar pitch volatiles (benzene



fractions containing anthracene, BaP, phenanthrene, acridine,



chrysene, pyrene, etc.).

-------
                                                                 41
3.   SOURCES


    3.1  Natural Occurrence


            Organic carcinogens are primarily unwanted by-products


    of imperfect combustion.  However, a few sources of organic


    carcinogens might be defined as naturally occurring.


            Bituminous coal contains BaP, benz(a)anthracene, and

              •p /- A
    other PAH.     However, studies in England reveal a lower


    incidence of lung cancer among coal miners than in the popu-


    lation at large.  '     Consequently, although carcinogenic


    agents are present in raw coal, they have no apparent bio-

                                264
    logical effect in that form.


            Asbestos miners, in comparison to coal miners, have

                                                  94
    a greater potential for developing malignancy.    Two of the


    three types of asbestos used industrially contain appreciable


    quantities of natural oils.  These oils, which adhere to


    asbestos particles, contain BaP-    The quantities (1 to


    5 |ag/100 g asbestos) are small enough that their presence

                                                    94
    may play no more than a minor carcinogenic role.


            Another potential naturally derived source of airborne

                                           262
    carcinogens has been reported by Troll,    namely, various


    molds in the environment that possibly produce toxic compounds,


    some of which may be carcinogenic.

-------
                                                             42
3.2  Production Sources



        Prior to the introduction of gasoline and diesel



engines, PAH were emitted primarily from the burning of coal



and other solid fuels used for home and industrial heating



as well as for the production of industrial products and



power.  However, in recent times the use of gasoline and diesel



engine fuels has resulted in a new major source of PAH.



        The U.S. Public Health Service, recognizing the need



for specific emission data, has obtained information concerning



those sources that involve burning conventional fuels and


                                              90,91
certain commercial and municipal solid wastes.  '    The



sources of heat generation that were tested ranged in size



from residential heaters to heavy industrial power plant



boilers and employed the following firing methods: (1) pulver-



ized coal burners, chain grate stokers, spreader stokers,



underfeed stokers, and hand-stoked coal burners; (2) steam-atom-



ized, centrifugal-atomized, and vaporized-oil burners; and (3)



premix gas burners.  The incineration sources tested ranged



from small commercial incinerators to large municipal inciner-



ators.  Open-burning sources and several industrial processes



were also tested.  From these studies, in conjunction with the



annual consumption and production figures, sufficient emission



data were collected to reveal the probable major sources of

-------
                                                             43
carcinogenic agents as indicated in Table 6, in the Appendix.




In interpreting the significance of this information, it




should be remembered that the calculation of total emissions




involved a considerable amount of estimation, as well as a




number of assumptions, with respect to both emission rates




and annual consumption or production figures.  Also, the




aggregate emissions from a number of small sources not con-




sidered in this study, although probably small, cannot be



          91
estimated.




        As shown in Table 6 in the Appendix, the sources were




separated into four major categories:  heat generation, refuse




burning, industrial processes, and motor vehicle operation.




This study revealed that each category contributes to the




atmospheric loading of BaP.  Generally, the importance of a




particular category or source has been evaluated in terms of




the United States as a whole, but the importance of a partic-




ular source, relative to concentrations found in the atmosphere,




undoubtedly varies considerably with locality; e.g., although




no coal is burned in Los Angeles County, the city of Los Angeles




has areas of exceptionally high traffic density-  Other factors




may also influence the concentrations of BaP and other poly-




nuclear hydrocarbons found in the atmosphere, such as decom-




position or reaction with smog constituents in the atmosphere.

-------
                                                              44
        The results and implications from these studies have




been summarized by Hangebrauck et. al.. 1 as follows:




        (1) Although each process surveyed undoubtedly contrib-




utes to the amount of BaP in the atmosphere, the most important




source of BaP is the inefficient combustion of coal, typified




by residential and small industrial coal-fired furnaces.




        (2 ) The efficient combustion of coal in modern




industrial-process heating boilers and power plants and the




combustion of fuel oil and natural gas do not appear to be




significant contributors.




        (3) In refuse burning, as in coal burning,  efficiency




of combustion governs the emission of polynuclear compounds.




Inefficient combustion in small incinerators as well as open




burning results in considerable formation of BaP and other




polynuclear hydrocarbons, whereas efficient combustion in




municipal incinerators results in very little BaP formation.




        (4) Results from direct and indirect sampling of




industrial sources, although not conclusive, indicate that




the following processes are not major sources of BaP:  an




asphalt air-blowing process (pyrene emissions were high),




an asphalt hot-road-mix plant, a carbon-black manufacturing




area,  a steel and coke manufacturing area, and a chemical




industry complex.

-------
                                                             45
         (5) Direct samples of the effluent from the catalyst


regenerators of petroleum catalytic cracking units indicate


that Houdriflow and Thermofor (air lift) units can be signifi-


cant industrial sources of BaP and other polynuclear hydro-


carbons.   Emission of these compounds can be, and frequently


is, reduced to negligible amounts through the use of CO-waste


heat boilers on individual "cat-cracker" catalyst regener-


ators.   Thermofor  (bucket lift) and Fluid units incorporate


catalyst regenerator designs that result in minor emissions


only.  Considerable additional testing would be necessary


to  statistically assure the evaluation of contributions


from catalyst regenerators, as for all of the sources tested.


         (6) Consideration of the emission rates determined for


gasoline-powered automobiles and trucks,  as well as of their


annual fuel usage, indicates that motor vehicles are a major


contributor of BaP in the atmosphere.  This is confirmed by

                     39
Colucci  and Bregeman,  who report automotive BaP contri-


butions  of 5 to 42 percent,  based on ratios of lead to BaP


in exhaust and in the atmosphere.  Evidence indicates that


old high-mileage vehicles and those with poorly adjusted


engines have the highest emission rates.


         (7) Limited data indicate higher emission rates for


gasoline-powered trucks than for automobiles.   Diesel-powered

-------
                                                              46
                                  251
trucks may also have higher rates;    however, diesel fuel



usage by trucks and buses accounts for only about 4 percent



of the total petroleum consumption for motor vehicles.  The



overall contribution from all trucks and buses, both gasoline



and diesel-powered, possibly exceeds that from automobiles,



although the total fuel usage for this category is about half



that for automobiles.



        See Section 3.3 for further discussion of PAH emissions



from automotive exhaust.



        A number of studies which deal with PAH emission from



specific types of  industries have been reported and are dis-



cussed briefly in  the following paragraphs.



        The PAH composition of air polluted by coal-tar pitch


                                  211
has been studied by Sawicki et al.     Coal-tar pitch pollution



is characterized by much higher ratios of pyrene to BaP, BaP



to benzo(g,h,i)perylene, and BaP to coronene, when compared


                                       213
with other types of pollution.  Sawicki    also suggested



that these ratios  are also probably characteristic of the two



types of pollution produced from the combustion of gasoline



and coal.



        Specific studies have been made of BaP levels in an


                     254
iron and steel works,    of PAH concentrations both in a gas


                  139                                    96
works retort house    and in stack gases from pulp mills,

-------
                                                              47
                              292
and of BaP in coke oven wastes    as well as in the vicinity



of a coal briquet factory.     The atmospheric emissions



produced by oil burners has been reported to be 0.04 to


                                             2 31
0.10 pounds of BaP per million pounds of oil.     Methods



for sampling from various coal-fired steam-generating instal-


                          54
lations are also reported.    A Russian study states that the



asphalt, concrete, tar roofing, and rubber industries are


                                                       45
carcinogenic foci with regard to pollution of city air.



        No detailed studies of the distribution of pollu-


                                             91 270
tants by prevailing winds have been reported.  '     However,



it can be inferred that after distribution by the winds, the



carcinogens fall back to earth.  If the carcinogens fall into



water, they may spread for considerable distances.  Il'nitskii



and Varshavskaya    in a review article have discussed PAH



determinations in ground water, streams, and harbors.  PAH



were also detected in the aquatic life of these waters.



3.3  Product Sources



        As discussed earlier.- exhaust from internal combustion



engines has resulted in a significant new source of PAH.  The



emission rate per gallon of fuel and the annual emission of



BaP are presented in Table 6 in the Appendix.



        Carcinogenic emissions from automotive sources can



be divided into at least two major categories: PAH, and the

-------
                                                             48
photochemical pollutants that are precursors of alkylating



agents.  For PAH emissions, Kotin and Falk    have reviewed



the literature pertaining to analysis of exhaust prior to



approximately 1963.  The primary conclusions are that a



broad spectrum of PAH, including several carcinogens, can be



detected in exhaust products; and the yield of PAH decreases



with the efficiency of the engine used.  In regard to the


                                      104
latter conclusion, Hoffmann and Wynder    have quantified



the BaP yield on the basis of engine mileage and found that



the BaP concentration varied from 5 |J.g/minute sample early



in engine life (8,000-12,000 miles) to 27 |ag/minute late in



engine life (29,000-33,000 miles).  When the diesel exhaust



from an engine operating inefficiently under maximum load at



varying rpm's was analyzed, BaP yield was found to vary from



876 i-ig/minute to 1,687 ng/minute.



        More recent studies on automotive exhaust products



have been conducted in the following areas:  (1) production



of PAH by cars and trucks, (2) contribution of automotive PAH



to total PAH, (3) carcinogenicity of automotive exhaust, and



(4) formation of carcinogens other than PAH.  Each of these



areas is discussed below in greater detail.



        The reports             dealing with the production



of PAH have fairly uniform conclusions.  For example:

-------
                                                         49
        (1) Automobiles with properly adjusted carburetors




generated and discharged less carbon monoxide and less BaP




into the atmosphere.




        (2) The highest concentrations of BaP and carbon




monoxide were discharged in exhaust gases from automobiles




operated at low rpm, usually at the time of starting or



                                                           159
accelerating.  Aldehyde emissions showed a similar pattern.




        (3) The concentrations of BaP and carbon monoxide




pollution in atmospheric air can be reduced substantially by




controlling carburetor operation at all times, particularly




when starting and moving into line with the traffic; keeping




the carburetor clean and well adjusted; and reducing the




number of stops and starts at crossings and light signals to




a minimum.  This can be accomplished with proper regulation of




traffic, as for example through better synchronized signal




lights and use of overhead and underground auto routes which




would allow automobiles to travel without making many stops.




        Several studies relating the contribution of auto-




motive PAH to total PAH have been reported.  A recent test



                               39
was conducted in Detroit, Mich.    The study showed that the




mean contributions by automobiles to the total amount of BaP




in the air at three sites were-  18 percent for freeway.-




5 percent for downtown, and 42 percent for suburbs (in winter)

-------
                                                             50
These results are based on the dilution factor of exhaust
concentration over atmospheric concentration found for lead.
Based on the carbon monoxide dilution factor, the results
were:  35 percent for freeway, 10 percent for downtown, and
32 percent for suburban areas (in winter).  An earlier study
from the same laboratory had estimated the PAH concentrations
            21
to be lower.
        The carcinogenicity of automotive exhaust has also
been studied.  '   '     Wynder and Hoffmann reviewed the
                                         2 86
pertinent literature prior to about 1962.     There is general
agreement that exhaust tars are carcinogenic to experimental
animals, the major portion of the activity is due to PAH, and
the emissions are dependent upon the type of fuel used.
        Another source of PAH is cigarette smoking.  (The
relation of cigarette smoking to cancer is briefly discussed
in Section 2.)
        Photochemically reactive compounds, which may be pre-
cursors of carcinogenic alkylating agents, are emitted as
by-products of incomplete combustion.  The major sources of
these pollutants are automobile operation and use of natural
fuels.
        The foregoing discussion outlines the major product
sources of carcinogens.  There are other reports in the

-------
                                                             51
                                                               O —j £-

literature of possible sources of carcinogens.  The Weisburgers




have reported that nitrosamines are included in 20 patented




products.  Rubber tire dust is also given as a source of BaP.




There are also several reports concerning petroleum products


                          229 265 290
which contain carcinogens.   '   '




3.4  Environmental Air Concentrations




        In a survey of 103 urban and 28 nonurban areas of the




United States in the late 1950's, the U.S. Public Health




Service found that concentrations of PAH were present in the


                                    213 217
atmosphere of all locations studied.   '     Selected values




for the winter months (January through March) of 1959 are



                                 119 217
given in Table 7 in the Appendix.   '     These values show




the striking contrast in urban and rural BaP concentrations.



                                              109 127 214
Additional recent studies in the United States   '    '



                    29 30 150 223 272
and other countries   '  '   '   '    have shown a similar




wide distribution and urban-rural differences.  The concen-




tration of nine various other PAH in particulate matter of




selected cities was measured in 1958-1959 (see Table 8, in



              213
the Appendix).     The data indicate that BaP is only a small




percentage of the PAH, ranging from approximately 5 to 20




percent of the nine PAH measured.




        There is also a wide seasonal variation in BaP con-




centrations in urban areas.  Data showing this variation are

-------
                                                             52
given in Table 9 in the Appendix.39'90'103  These data
are
relatively outdated, yet they have been presented in recent
                                                 1 88
surveys by Hoffmann and Wynder    and by Sawicki .     Sim-



ilar surveys have been conducted in Australia,   Belgium,



Canada,150 Czechoslovakia,76'230 Denmark,29 Germany, 100' 131



Great Britain,30'249'271'273 Hungary,185 Italy,18'161'266'



        the Netherlands,   Norway,   Poland,    South Africa,



146 and Sweden.73



        Concentrations of BaP in 106 urban and nonurban areas



of the United States were measured by the National Air Sampling


                4
Network in 1966.   These data are tabulated in Table 10 in the



Appendix.  The maximum station average value reported was



0.0112 |~ig/m3 for urban areas and 0.00145 ug/m3 for nonurban



areas; the arithmetic average value was 0.00279 ug/m3 and



0.00035 |J.g/m3, respectively.

-------
                                                                 53
4.   ABATEMENT




            Emissions of PAH are generally associated with con-




    ditions of incomplete combustion of carbonaceous materials.




    Emission sources include motor vehicles, refuse burners, and




    domestic and industrial facilities that burn coal, oil, or




    gas.  Methods presently being studied to control these




    emissions include more efficient techniques for combustion




    of fuels, collection or trapping of emissions  (particularly




    in the form of particulates), and use of combustion treat-




    ment techniques for the emissions (such as direct-flame or




    catalytic afterburners).  In a 1967 study of polynuclear



                                           91
    hydrocarbon sources, Hangebrauck et_ .al_.   made the following




    comments on abatement for particular sources:




            (1)  Heat generation.  Replacement of inefficient




    coal-fired furnaces would greatly reduce emissions attrib-




    utable to heat generation.




            (2)  Motor vehicles.  Control systems applied to




    automobiles and trucks for the purpose of reducing emissions




    of total gaseous hydrocarbons should reduce emissions of BaP




    and other polynuclear hydrocarbons, but no data are available




    to prove that this is possible.




            (3)  Refuse burning.  The burning of all refuse




    (especially that with a high ratio of hydrogen to carbon) in

-------
                                                             54
modern municipal incinerators or disposal by noncombustion




methods would almost eliminate emissions attributable to



refuse burning.



                          91
        Hangebrauck et al.   found that emissions of poly-




nuclear hydrocarbons from the catalytic regenerators of




petroleum catalytic cracking units can be reduced to negli-




gible amounts through the use of carbon monoxide-waste heater




boilers.  The data  (see Table 6 in the Appendix) indicate




well over 99 percent reduction upon passage through the




carbon monoxide boiler.




        In addition, fuels containing BaP and other carcino-




gens may be thermally pretreated before use to remove or


                                            292
destroy these carcinogens.  Yanysheva .et_ _al_.    reported




success in removing BaP from fuel bricks by heat treatment.




Heating the fuel bricks in an electric oven at 60 C for




2 hours and 15 minutes reduced the BaP content  from an




average of 0.07 percent BaP to 0.0000017 percent.

-------
                                                                 55
5.  ECONOMICS




            No information has been found on the economic costs




    of organic carcinogen air pollution.  However,  in 1967,



          169
    Ridker    estimated the cost to the economy of diseases




    associated with air pollution, including cancer of the respir-




    atory tract.  Although this study does not indicate the rela-




    tive contribution of organic carcinogens as compared with




    inorganic carcinogens, occupational hazards, smoking, etc.,




    the study does demonstrate that cancer as a result of air




    pollution may result in significant economic losses.  According




    to Ridker,    the costs of a disease may be classified ac-




    cording to four categories:  (1) those due to premature death,




    (2) those associated with morbidity (i.e., burial),  (3) those




    incurred for treatment, and (4) those incurred for prevention




    or avoidance.




            For the first category, Ridker reports a model that




    estimates the loss of output due to premature death.  While




    Ridker elaborates considerably on the assumptions (using 1958




    as a base year), the mathematical model is as follows:




                        oo    p  *  p^  •  p^ •  V

                              a.    a     a     n

                V   =   £      123



                                 (1 + r)n ~ a

-------
                                                             56
where

        Va   is the present value of the future earnings of
             an individual at age a;

        P    is the probability that an individual at age a
          1  will live to age n;

        P&   is the probability that an individual at age a,
          2. living to age n, will be in the labor force at
             age n;

        Pa   is the probability that an individual at age a,
          3  living and in the labor force at age n, will be
             employed at age n;

        Y    is the earnings at age n; and

        r    is the rate of interest.

        The costs of premature death were calculated from

statistical data for the labor force.

        Burial costs have been similarly calculated.  If

death is postponed/ society delays using resources for burial.

The gain thereby obtained is the difference between the present

cost of burial and the present value of the expected cost of

future burial.  This can be calculated from the following

formula:
        c
        "-a
1 -  S
                                  Pn
                                   a

-------
                                                             57
where

        Ca   is the present value of the net expected gain
             from delaying burial at age a;

        C    is the cost of burial;
         n
        Pa   is the probability that an individual at age a
             will die at age n; and

        r    is the discount rate.

        The costs of treatment and absenteeism were esti-

mated from specific data derived from a variety of sources.

        As indicated in Table 2, cancer of the respiratory

system is the most costly of these diseases.

        No information has been found on the economic costs

of the abatement of organic carcinogen air pollution.

-------
                                      TABLE 2
        RESOURCE COSTS PER YEAR OF DISEASES ASSOCIATED WITH AIR POLLUTION
                               (Millions of Dollars)a
                                                                         169
                             Costs Associated with Selected Diseases
Type of
Cost
  Cancer
  of the
Respiratory   Chronic      Acute     Common
  System     Bronchitis  Bronchitis   Cold   Pneumonia  Emphysema  Asthma
Premature
  death

Premature
  burial

Treatment

Absenteeism
    518


     15


     35

    112
 18
  0.7
 89

 52
6


0.2


b

b
 b
200

131
329


 13


 73

 75
62
 b

 b
 59
138

 60
  Total
    680
159.7
6.2
331
490
64
259
       Using a discount rate of 5 percent.

       DNot applicable.
                                                                                             00

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                                                                 59
6.  METHODS OF ANALYSIS


            Several publications have compared methods and pro-


    vided an overview of the role of analytical chemistry in


    carcinogenesis studies.177'187'215'262'276  The methods of


    analysis generally consist of the following sequence of


    steps:  (1) sampling,  (2) extraction,  (3) separation, and


    (4) analysis.


    6.1  Sampling Methods


            Organic carcinogens are usually associated with par-


    ticulates and thus are collected as  "particulates."  Sampling


    is often accomplished with a  "high-volume" air sampler, the


    particulates  (with which the relevant compounds are associated)


    being collected on a glass-fiber filter.  Collection into a


    liquid  (cyclohexane) absorbent has also been employed.  The


    collection process may continue for  many days, in order to


    obtain  sufficient quantities of material.  A technique which


    has proved satisfactory for collecting BaP from high-temperature


    gas streams is the use of a series of water bubblers and con-


    densate traps immersed in an ice-water bath, followed by a

                           238
    high-efficiency filter.


    6.2  Extraction Methods


            The organic carcinogens are  generally removed from


    the particulates by solid-liquid extraction, although liquid-

-------
                                                             60
liquid extraction, sublimation, and distillation (for coal-tar

                               1 p-y
mixtures) have also been used.



       Solid-Liquid Extraction.  Particulates are commonly



extracted from the atmosphere with either benzene or cyclo-


       187
hexane.     Other solvents used include chloroform, acetone,



isooctane, methanol, and dimethylformamide, as well as benzene



mixtures of aliphatic hydrocarbons or methanol.  The solvent-



particulate mixtures can be stirred at a certain temperature



or a Soxhlet extraction can be used.



        Recent studies have been concerned with the efficiency


                               233
of extraction.  Stanley et al.    showed that in the analysis



of equal weights of air particulates enriched with BaP,



benz(c)acridine, and 7H-benz(de)anthracen-7-one, the percent-



ages of these compounds extracted ranged from 50 to 100, 15



to 100, and 40 to 80, respectively.  The solvents were cyclo-



hexane, benzene, methylene chloride, and acetone.

                                      CO

        In another study Dubois et al.   stated that benzene



was of questionable value as  an extracting agent for the



initial preparation of an air  sample.  The larger amounts of



material extracted by benzene, as compared with cyclohexane,



lead to analytical interferences in the subsequent analyses.


                2
Aigina and Mints  found somewhat similar results in that the



extinction of BaP fluorescence called for a controlled content

-------
                                                             61
of 1, 12-benzoperylene as an internal standard.
6.3  Separation
        Techniques used for separating the different organic
carcinogens include column chrotnatography, thin-layer chroma-
                                 187
tography, and gas chromatography.
6.3.1  Column Chromatography
        Column chromatography is the common method for sepa-
ration of a complex mixture of PAH.
        Column chromatography techniques are sufficiently
advanced that predictions can be made with regard to adsorba-
                                                      187
bility of hydrocarbons on alumina.  Structural studies
have shown that adsorbability is greater for:
        (1)  A compound with more rings; e.g., chrysene is
adsorbed more strongly with phenanthrene;
        (2)  A compound with more double bonds; e.g.,
benz(a)anthracene is adsorbed more strongly than pyrene;
        (3) An acene (a linearly condensed arene) than for
an isomeric phene (an angularly condensed arene); e.g.,
naphthacene is adsorbed more strongly than benz(a)anthracene;
        (4) Fluorenic hydrocarbons than for pericondensed
hydrocarbons with the same number of rings and double bonds;
e.g., HH-benzo(b)fluorene is adsorbed more strongly than
pyrene;

-------
                                                             62
         (5)  The most nearly coplanar compound of a group;




e.g., the decreasing order of adsorbability, 2-phenylanthra-




cene>l-phenylanthracene>9-phenylanthracene/ follows the in-




creasing angle of twist  in these molecules;




         (6)  A hydrocarbon substituted with an increasing




number of sterically unhindered methyl or  alkylene groups




than for the hydrocarbon itself; e.g., 9-methylanthracene is




adsorbed more strongly than anthracene; and




         (7)  The most extensively conjugated isomer; e.g.,




l-(2-naphthyl)cyclopentene is adsorbed more strongly than




3-(2-naphthyl)cyclopentene.




        It is postulated  that adsorption on alumina involves




pi-type complexation where the active sites on the alumina




are relatively broad electron-attracting areas to which the




electron-donating hydrocarbon substrate is held monomolecularly




and preferentially in a  planar configuration parallel to the




surface, if such arrangement is sterically possible.




        In general, the  columns and techniques used are com-




promises between better  separations and analytical speed.  '    '



195 200 209 210
   '    '   '     For example, the cyclohexane elution of an air




sample can take two weeks and require 500 to 600 fractions




totaling 5,000 ml.  To speed up the process it is customary




to add increasing amounts of ethyl ether.   This speeds the

-------
                                                             63
removal of solutes from the column but at the expense of


           59
resolution.    The chromatographic separation can also be



speeded by the use of alumina deactivated by the addition



of 1.6 to 1.8 percent water.  If the alumina were not deacti-


vated to this extent, the PAH would come off the column too

       59
slowly-


                                                       O "1 Q
        Hydrocarbons are eluted in the following order:


aliphatics, olefins, benzene derivatives, naphthalene deriva-



tives, dibenzofuran fraction, anthracene fraction, pyrene



fraction, benzofluorene fraction, chrysene fraction,  benzo-


pyrene fraction, benzoperylene fraction, and coronene frac-


tion.  For example, with alumina containing 13.7 percent



water,- the pyrene fraction was found in the beginning of the


3 percent ether eluent; the chrysene fraction followed in the



last part of the 3 percent ether eluent; the benzopyrene


fraction appeared in the beginning of the 6 percent ether


eluent, followed by the benzoperylene fraction in the start


of the 9 percent ether eluent, and the coronene fraction


shortly afterward in the same 9 percent ether eluent.  Al-


though the relative location of the fractions was always the



same, unknown variables sometimes caused the fractions to



elute sooner or later than expected.  The fractions were



fairly well separated,  although test tubes containing the

-------
                                                             64
tail end of one fraction usually contained small amounts of
the next fraction.  Most fractions were found in three to six
tubes.  However, the benzoperylene and coronene fractions were
each spread over 6 to 10 tubes.
        Column chromatography studies have also been reported
                 195 200
by Sawicki et al.f   '    in which the separation of polynuclear
aza-heterocyclic hydrocarbons and polynuclear aromatic amines
was considered.  The procedures are essentially the same as
for PAH.  However, the alumina does cause some decomposition
of the amines; e.g., 9-aminoanthracene is recovered in 70 per-
cent yield as anthraquinone, while 2-aminoanthracene is in
only 26 percent yield.
6.3.2  Thin-Layer Chromatography
                                  187
        In his 1964 review Sawicki    noted that although
thin-layer chromatography had been applied only to a small
extent in the separation of PAH, it would find increased
use; since that time there have been a sizable number of
                                                 13—15 143
thin-layer chromatography (TLC) studies reported.     '   '
1 Q1 9 OR 0 "\A. 977
±yj.-tva,tj
-------
                                                              65
of developing solvents have been evaluated for use in TLC.



Several authors   '    '    '    state that the complete analysis




procedure generally consists of extraction followed by separ-




ation using either TLC or paper chromatography, and then spot



                                              193
location by fluorescence or color development.     One reagent



used for color development is 7,7,8,8-tetracyanoquinodimethan,




which reacts with polynuclear compounds to form colored pi



complexes.


                        277
        White and Howard    give R  values (the ratio of the



migration distance of the substance to the migration distance




of the solvent front) for 29 PAH on cellulose and cellulose



acetate adsorbents.   Such values, however, are quite specific



for a given adsorbent and developing solvent.  The R  values
                                                    c


alone are inadequate  for characterizing polynuclear com-




pounds.207



        The efficiency of separation and recovery by TLC has




also been studied.13'202'206'234  Typical results are those


                          O (~\O O r*\£.

reported by Sawicki et al.   '    in which recoveries of



65 percent for 9-acridanone and 85 percent for BaP were ob-




tained.  Assays for these same compounds were also run using



fluorometric procedures after TLC separation.  It was possible


                                                        O (~\ O

to detect 9-acridanone in concentrations of  .0004 p.g/m3 .     For


                                                            O (~\£i

BaP the identification limit ranged between zero and .04 |Jg.

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                                                              66
Previously Sawicki  and  Johnson192  had  reported  the following



identification limits:  anthracene,  0.01  ug;  phenanthrene,



1.0  ug;  fluoranthrene,  0.01  ug;  chrysene,  0.01  g.g;  pyrene,



0.001  |jg;  and  BaP,  0.001  u.g.



         The separation  and characterization  of  polynuclear



aza-heterocyclic  hydrocarbons  and  polynuclear aromatic  amines



have also  been reported.14'15'204'208  The identification



limits are in  the same  range as  for PAH;  examples  are:  car-



bazole,  0.02 ug;  llH-benzo(a )carbazole,  0.02 |ag; 1-azacarbazole,



0.2  ug;  and 1,2-dinaphthylamine,  0.1 ug.



6.3.3  Gas Chromatoqraphy



         A  limited number  of gas  chromatography  studies  have



been conducted.31"33'53'67'279  Although  the procedures are



still  in the process  of being  worked out,  the method promises



to be  relatively  rapid, provided the various chromatographic



peaks have been identified.  As  an  example of the  capabilities



of the method,  the  following amounts of PAH in  soot have been


         ^ o
reported:    acenaphthylene, 4,700  Ug/g soot; phenanthrene,



5,500 Ug/g soot;  fluoranthrene,  5,200  ug/g soot; chrysene,



6,300 ug/g soot;  and  BaP, 2,900  Ug/g soot.



6.3.4  Other Techniques



         Electrophoresis has also been  considered a possible


                                                82 212
means of separating pollutants after collection.   '

-------
                                                              67
                                                    212
Reports show that separations of polynuclear phenols    and



of macromolecular material82 are feasible; however, no



quantitative information is given.



6.4  Analysis




        Analyses of the eluate fractions from chromatography



are conducted chiefly via ultraviolet absorption measurements


                             187
or fluorescence measurements.     Presently, the National Air


                                O fc.

Pollution Control Administration   analyzes airborne partic-



ulates for BaP by a fluorescence method (a modification of


                                   210
the one described by Sawicki et al.   ) using thin-layer



chromatography for separation (see References 234 and 206).



        The following discussion briefly reviews the various



analytical procedures that are applicable to the study of



airborne carcinogens.



6.4.1  Spectral Methods



        Methods based on ultraviolet-visible absorption spectra



and fluorescence spectra play a prominent role in the identi-



fication and determination of polynuclear hydrocarbons.  The



advantages of absorption spectra are that the wavelength



maximum of a compound is unaffected by the presence of other



compounds, quenching effects are absent, and the absorption



curves of many PAH are available in the literature.  The main



disadvantage, compared to fluorescence, is the lower sensi-



tivity-  For many fluorescent compounds, the excitation and

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                                                             68
emission spectra are 10 to 10,000 times more sensitive than



their absorption spectrum.  The disadvantages of fluorescence



spectra are the poorer reproducibility, the fewer available



quantitative spectra, and quenching that can drastically affect



the sensitivity and shape of the spectral curve.187



        Several recent articles have dealt with spectral



methods.  Of these, one was a preliminary communication con-



cerned with the detection of impurities in commercial poly-


                           259
cyclic hydrocarbon samples.     The remaining articles are


                                                         118
concerned with the methods of ultraviolet-visible spectra   '



129,156,189,216 and fluorescence spectra.194'205  The results




of these studies are summarized in Table 3.



6.4.2  Other Methods


                                    o ~i a.

        In one study, Sawicki et al.    also investigated the



application of the piperonal test to the benzene-soluble



fraction of airborne particulates.  Piperonal reacts with



aromatic compounds to give a colored product which obeys



Beer's law.  The correlation coefficients between BaP concen-



trations and the piperonal test were 0.95 for 174 urban samples



and 0.89 for 25 nonurban samples.



        Two papers deal with thermochromic tests for deter-



mining the amounts of polynuclear compounds containing the


                         190       .     ,.       .       203
fluorenic methylene group    and polycyclic p-quinones.

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                                                                 69
                             TABLE  3

      SUMMARY OF RECENT  INVESTIGATIONS  IN SPECTRAL METHODS
Method
Compound(s) Procedure
Normal Range
of
Sensitivity
Refer-
ence
Ultraviolet  Benzo (a )pyrene      Column     1  to  6  |ag/ml      156
                             chroma tography

Ultraviolet  DiLenz (a, j] -        Hexane     0.5 to  5 l-ig/ml     118
             anthracene        extraction

Ultraviolet  Benzo (a )pyrene     Benzene     0  to  45 [ig/1,000  216
                               extraction     /m3  air
Ultraviolet  Benzo(a)pyrene      Hexane      10~2  ug/ml         129
                               extraction

Ultraviolet  Benzo (a)pyrene     Modified    0.003 p, moles/ml  189
             Pyrene             Piperonal   0.01  p. moles/ml
             Perylene             tests      0.001 (J. moles/ml

Fluorescence Benzo (a)pyrene  Acetophenone-  2.5 x 10~°  M      205
             Perylene        trif luoroacetic 10~" M
                               acid

Fluorescence Perylene           Pentane      0.3 M-g/ral          194
             Anthanthrene      extraction   0.3 ug/ml
             Benzo(a)pyrene                 0.4 |J.g/ml

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                                                             70
In both cases borohydrides were used at reflux temperatures



for color development.  Identification limits ranged from



3 to 40 [ag.  A somewhat similar study was reported in which



4-azobenzenediazonium fluoroborate was used to develop color



in the determination of aniline, naphthylamine, and anthramine



derivatives.  The identification limits were in the range of



2 to 20 |jg.



        A direct method of determining BaP has been devised



using its characteristic quasi-line emission spectrum at


                             160
liquid nitrogen temperatures.     The method has been used



to determine BaP in the air of submarine crew quarters, typ-



ically about 1 ppm.



        Bioassay techniques have been reported in which the



response of a microorganism is correlated with carcinogenic



activity.63'65'66'282  These techniques are based on the



phenomenon known as photodynamic action where a combination of



light energy and chemical sensitizer (in this case PAH) causes



the immobilization and death of Paramecium caudatum.  Defini-



tive assay for BaP can be performed in the 0.001 to 100 ug/ml



region.

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                                                                 71
7.  SUMMARY AND CONCLUSIONS




            Epidemiological studies indicate a possible relation-




    ship between air pollution and lung cancer mortality in the




    United States and other countries.  While the role of cigarette




    smoking is clearly better defined, the role of air pollution




    and possibly of organic carcinogens  is less certain but is




    still believed to be present.  The existence of urban-rural




    differentials and migrant-native differentials speak of a




    possible contribution of air pollution to total lung cancer




    mortality.




            Animal experiments indicate that under certain con-




    ditions many of the organic carcinogens found in ambient air




    can produce tumors.  In most of the experiments, the compounds




    were painted on the skin.  However, these investigations have




    shown that synergistic and antagonistic effects are important.




    Thus, some compounds, such as phenols, have been found capable




    of promoting or reinforcing the action of organic carcinogens.




    Inhalation experiments indicate that benzo(a)pyrene (BaP)




    adsorbed on inert particulates (iron oxide) causes a higher




    incidence of lung tumors in hamsters than just BaP alone.




    Moreover,  these tumors were of the same types observed in




    humans.  In contrast, many noncarcinogenic polynuclear aro-




    matic hydrocarbons (PAH) can reduce the potency of organic

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                                                          72
carcinogens, at times to such an extent that no effect is



found.




        No information has been found in the literature con-



cerning the effects of airborne organic carcinogens on plants



or materials.




        The atmospheric carcinogens  fall into three principal



categories, namely, PAH, polynuclear heterocyclics and



oxygenated compounds, and alkylating agents.  There are very



little data available on cocarcinogens and anticarcinogens



that may be present in the air.



        The major  sources of PAH are heat generation, refuse



burning, industrial processes, and motor vehicles.  Of these,



heat generation accounts for more than 85 percent of the PAH



emitted, with the  other three sources each accounting for



about 5 percent of the total.  Similar figures for other



organic carcinogens have not yet been estimated.



        Sources of aza-heterocyclics and polynuclear carbonyl



compounds have not been well established.  Combustion prod-



ucts such as coal  tar are likely sources.



        Only limited data are available on the concentra-



tion of organic carcinogens in the ambient air.  In 1966,



the concentrations of BaP in 106 urban and nonurban areas



were measured0  The average value was 0.00279 |_ig/m3 for



urban areas and 0.00035 |_ig/m3 for nonurban areas.  Other



data indicate that BaP constitutes only a small'percentage

-------
                                                          73
(as low as 5 percent) of the total PAH present in the



atmosphere.




        Control methods for organic carcinogens are being



studied in connection with hydrocarbon and particulate con-



trol programs.




        The costs of premature death attributable to air



pollution and the associated costs of treatment and burial



have been estimated.  These costs for cancer of the respira-



tory system were estimated to be $680 million per year on



the basis of 1958 data.  However, no information has been



found on the economic costs of organic carcinogen air pollu-



tion or on the costs of its abatement0



        Determination of organic carcinogens (mainly PAH)



commonly involves separation of the different PAH by chroma-



tographic techniques, followed by analysis by ultraviolet-



visible absorption spectra, fluorescence spectra, or phos-



phorescence spectra.



        Based on the material presented in this report,



further studies are suggested in the following areas:



        (1)  Expansion of current efforts to determine the



atmospheric concentrations of organic carcinogens.  Specific



airborne carcinogenic material as well as the composition of



hydrocarbon mixtures should be identified.  Particle size



and geographical distribution should also be determined.

-------
                                                          74
        (2)  Expansion of animal research to resolve the role



of airborne carcinogens, cocarcinogens, and anticarcinogens



in pulmonary human cancer.



        (3)  Determine the significance of the role of



organic carcinogens in the so-called "urban factor."

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                                                                   75
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                                                                   77
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                                                                    80
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-------
                                                                     91
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APPENDIX

-------
APPENDIX
                                99
                         HC = CH
                           I
                           H-
                           I
C32H66
                                          C10H22
                                     (plus other hydrocarbons)
                        (Cg - C2 unit)
                              Benzo(a)pyrene
                                FIGURE  2

   Benzo(a)pyrene Pyrosynthesis  (as Suggested by Badger)
                       103

-------
APPENDIX
        100
                 u

                            FIGURE 3




       Pathways for the Pyrosynthesis of Benzo(a)pyrene
103

-------
     Chrysene
  Benzo(e)pyrene
  (1, 2-benzopyrene)
Benz(e) acephenanthrylene
(3, 4-benzofluoranthene)
Dibenzo(e, I)pyrene
(1, 2, 3, 4-dibenzopyrene)
  Benz(a)anthracene
  (1, 2-benzanthracene)
  Benzo(a)pyrene
  (3, 4-benzopyrene)
Benzo(j)fluoranthene
(10, 11-benzofluoranthene)
Dibenzo(a, h)pyrene
(3, 4, 8, 9-dibenzopyrene)
lndeno(1, 2, 3-cd)pyrene
(o-phenylenepyrene)
Dibenzo(a, i)pyrene
(3, 4, 9, 10-dibenzopyrene)
                                                                                                                 ti
                                                                                                                 td
                                                                                                                 §
                                                                                                                 H
                                                                                                                 X
                                               FIGURE  4

                      Carcinogenic Polynuclear Aromatic Hydrocarbons
                                  Identified in  Urban  Air103
                                                                                                                  o

-------
APPENDIX
                                                                            102
                        N
              Dibenz(a, h)acridine
              (1, 2, 5, 6-dibenzacridine)
          N
  Dibenzla, i)acridine
  (1, 2, 7, 8-dibenzacridine)
                        O
                         I
                   Anthanthrone
     Phenalene-9-one
                        O
                        in
                  Xanthene-9-one
        O
         IV"
7H-benz(de)!anthracen-7-one
                                 FIGURE 5
            Aza-Heterocyclics and  Polynuclear Carbonyl
                Compounds  Identified in  Urban Air
                                                        103

-------
       N02 + hv 	* NO + 0

                        0 H
                       O—0     HJD
CH2 — CH2 + O3	^ ^ 2\    CH2
                                     HO — CH,— O— O — CH,— OH
   CH,
R - C = CH2 + 0-
r CHs -i
R —
-
C
R-(


C — CH2
\J

^>

CH3
I
1
R — C+— OO"
\^
t
+ HCHO

'H3 ^^ CH3
: — CH, R-C = o 4
V/
O
                                                     H20
                        FIGURE 6

Reaction Pattern  Showing the Products of Decomposition of
Unsaturated Hydrocarbons.   Identification Accomplished by
Infrared Spectroscopy and Gas Chromatography.-'-°°
                                                                                  o
                                                                                  co

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                                           TABLE 4

                  TUMOR INDUCTION IN MICE FOLLOWING CUTANEOUS ADMINISTRATION OF
                     ORGANIC EXTRACTS OF PARTICULATE ATMOSPHERIC POLLUTANTS64
Particulates   Extract
            Administration
                     Latent
                     Period    % Tumor Yield
                    (months)   Local   Distant
                     Comments
Filtration and Benzene-
precipitation  ether

Large capacity Benzene
collectors
Filtration and Dichloro-
sedimentation  ethane
Large volume
collectors

Ventilation
filters
Oxidation
products of
aliphatics

Filtration of
city smoke
Benzene
Benzene
and 2
fractions

Benzene
Benzene
           Subcutaneous x 1       12       6
           (-50,000 ug)

           Subcutaneous x 1       16       8
           (-50,000 ug)
Painting 3 x wkly       6      38
(10% benzene sol.)

Painting 3 x wkly      15      42
(acetone sol.)

Painting 3 x wkly       ?      45
(1% benzene sol.)
Painting 3 x wkly      14      20
(acetone sol.)
Painting 6 x only      18      20
(1% benzene sol.)
                                                Multiple
                                                Adenomas
Multiple
Adenomas
  16
Particulates from 5
urban sites

Similar tumor yields
from samples of 5
urban sites

Particulates from 3
urban sites

Particulates from
Los Angeles

Particulates from
Liverpool, England
                Aromatic-free aero-
                sol collected in
                Shepherd traps

                Particulates from
                Newcastle, England
                                                                                        continued)

-------
APPENDIX
                                      TABLE  4  (Continued)

                  TUMOR INDUCTION  IN MICE  FOLLOWING  CUTANEOUS  ADMINISTRATION OF
                     ORGANIC EXTRACTS OF PARTICULATE ATMOSPHERIC POLLUTANTS
Particulates
Extract
Administration
Latent
Period
(months)
% Tumor Yield
Local
Distant
Comments
Composite
NASN
samples
Composite
NASN,  camp,
samples
Benzene    Subcutaneous x 24     9-26
and 3      (organic ~130,000 |ag )
fractions  (aromatic ~13,000-
           33,000 ug) (oxygenated
           -12,000 |ag) (aliphatic
           -24,000 |ag); Painting
           2 x wkly (aliphatic
           and oxygenated)

Benzene    Subcutaneous x 3      3-12
           to neonates (15,000-
           25,000 uq)
2-10
       Hepatomas,
       8-83;  Lympho-
       mas,  1-17;
       Multiple
       adenomas, 13-
       75
Different tumor
yields from samples
of 8 urban sites.
In general, low
tumor yields
obtained
Different tumor
yields from samples
of 6 urban sites.
Results suggest role
of several carcino-
gens
                                                                                                    o
                                                                                                    Ln

-------
                                           TABLE 5

     TUMOR INCIDENCE FOLLOWING INJECTION OF  ORGANIC ATMOSPHERIC POLLUTANTS TO NEONATAL MICE
64
Groups
Series
Un injected controls

Solvent controls

Chicago '63 (10024)

Cincinnati ' 63
(10025)
Los Angeles '63
(10026)
Dose (|_ig).
of Organic
Pollutants
0

0

25 , 000

25,000

25,000

No.
Neonates
Iniected
90

100

117

123

77

Sex
M
F
M
F
M
F
M
F
M
F
No.
at
Weaninq
36
37
47
42
42
37
32
31
27
22
No.
at
RiskD
30
36
44
39
22
37
16
27
11
21
o/
/o
Pulmonary ,
Solitary
13
3
11
8
27
19
25
11
36
5
Tumor Incidence3
Adenomas,
Multiple
0
0
0
0
50
19
63
74
27
10
Hepa-
tomas
7
0
2
0
14
0
31
4
9
0
Lympho-
- ma
0
0
0
0
5
8
6
15
18
5
        aTests terminated  at  each 50  weeks.

        •'-'Excluding deaths  <50 weeks from unrelated causes,  notably obstructive renal failure in
males, also losses due to  cannibalism or autolysis.

-------
                                TABLE 6
ESTIMATED ANNUAL BENZO(A)PYRENE (BAP) EMISSIONS FOR THE UNITED STATES
                                                                     91
 Source
Estimated BaP
  Emission
    Rate
Estimated Annual
  Consumption
 or  Production
Estimated Annual
  BaP Emission
     (tons)
Heat generation
Coal
Residential
( i ) hand- stoked
( ii ) underfeed
Commercial
Industrial
Electric generation
Oil
Gas
Total
Refuse burning
Incineration
Municipal
Commercial
Open burning
Municipal refuse
Grass, leaves
Auto components
Total
(^g/106 Btu)


1,400,000
44,000
5,000
2,700
90
200
100

( jag/ton )

5,300
310,000

310,000
310,000
26,000,000

(1015 Btu)


0.26
0.20
0.51
1.95
6.19
6.79
10.57

(106 tons)

18
14

14
14
0.20




400
9.7
2.8
5.8
0.6
1.5
1.2
421.6


0.1
4.8

4.8
4.8
5.7
20.2
                                                                          (continued)

-------
                                        TABLE 6 (Continued)

            ESTIMATED ANNUAL BENZO(A)PYRENE (BAP)  EMISSIONS FOR THE UNITED STATES
           Source
                                         Estimated BaP
                                           Emission
                                             Rate	
Estimated Annual
  Consumption
 or  Production
 Estimated Annual
   BaP Emission
	(tons)	
Industries
  Petroleum catalytic
  Cracking (catalyst regeneration)
                                                                  (10° bl)
   (i)
   (ii)
   HCCC
   (i)
   (ii)
   TCCa
   (i)
   (ii)
    cc
        no Co boiler
        with CO boiler

        no CO boiler
        with CO boiler
        (air lift)
        no CO boiler
        with CO boiler
       (bucket lift)
   (i)  no CO boiler
   (ii) with CO boiler
  Asphalt road mix
  Asphalt air blowing
  Carbon-black manufacturing
  Steel & Coke manufacturing
  Chemical complex
240
14
218,000
45
( iag/bl )
90,000
<45

<31
50 |ag/ton
<1 0,000 ug/ton
790
790
23.3
43.3
(106 bl)
131
59
119
0
187,000 tons
4,400 tons
0.21
0.012
5.6
0.0024
13.0
<0.0029
0.0041
0
0.000010
<0. 000048
                                             Atmospheric samples indicate that BaP  Emissions
                                                      from these processes are not
                                                            extremely high
Total
                                                                                       18.8
                                                                                     (continued)
                                                                                                   o
                                                                                                   OD

-------
                                TABLE 6 (Continued)




       ESTIMATED ANNUAL BENZO(A)PYRENE (BAP) EMISSIONS FOR THE UNITED STATES
Source
Motor vehicles
Gasoline
Automobiles
Trucks
Diesel
Total
Total (all sources tested)
Estimated BaP
Emission
Rate
170
>460
690


Estimated Annual
Consumption
or Production
(1010 gal)
4.61
2.01
0.257


Estimated Annual
BaP Emission
(tons)
8.6
2.0
>20.6
481
d
 FCC:  fluid catalytic cracker.



 CO  boiler: carbon monoxide waste heat boiler,



"HCC:  Houdriflow catalytic cracker.



 TCC :  Thermo for catalytic cracker.
                                                                                           O

-------
APPENDIX
                                                                      110
                      TABLE 7

      BENZO(A)PYRENE CONCENTRATIONS IN URBAN
SAMPLING SITES  FOR JANUARY THROUGH MARCH  1959
                                                          J-iy '
State
Alabama
Alaska
Arizona
Arkansas
California




Colorado
Connecticut


Delaware
District of
Columbia
Florida


Georgia
Illinois

Indiana



Iowa
Kansas

Kentucky
Louisiana
Maine
Maryland
Massachusetts



Michigan

City
Montgomery
Anchorage
Phoenix
Little Rock
Berkeley
Glendale
San Bernardino
San Diego
San Jose
Denver
Hartford
New Britain
New Haven
Wilmington

Washington
Miami
Orlando
Tampa
Savannah
Chicago
Rockford
East Chicagoa
Indianapolis
Hammond
South Bend
Des Moines
Topeka
Wichita
Louisville
Shreveport
Portland
Baltimore
Boston
Lowell
New Bedford
Worcester
Dearborn
Flint
Grand Rapids
ng BaP/g
of Particulate
340
64
15
20
41
5.3
13
20
7.2
51
68
50
53
55

71
28
110
140
49
74
63
34
120
280
91
160
40
19
70
6.4
180
64
45
40
81
66
110
140
91
ug BaP of
Benzen,e Soluble
Fraction
2,000
540
160
230
260
38
140
150
91
290
730
450
580
650

59
250
810
1,200
480
950
660
710
1,100
2,600
200
1,600
510
310
860
150
2,100
650
730
410
1,000
700
960
1,400
1,400
lag BaP/
m3 air
.024
.0038
.0050
.0015
.0029
.0008
.0023
.0021
.00056
.0069
.0065
.0055
.0053
.010

.0093
.0019
.011
.015
.0043
.015
.0073
.0111
.026
.039
.016
.023
.0031
.0023
.016
.00065
.021
.014
.0096
.0031
.0044
.014
.0090
.015
.015

-------
APPENDIX
111
                          TABLE  7  (Continued)

                 BENZO(A)PYRENE  CONCENTRATIONS  IN URBAN
              SAMPLING SITES FOR JANUARY THROUGH  MARCH 1959
State
Minnesota

Mississippi
Missouri

Montana
Nebraska
Nevada
New Hampshire
New Jersey



New Mexico
North Carolina

North Dakota
Ohio





Oklahoma
Oregon
Pennsylvania






Rhode Island
South Carolina

South Dakota
Tennessee

Texas




City
Duluth13
Minneapolis
Jackson
Kansas City
St. Louis
Helena
Omaha
Las Vegas
Manchester
Bayonne
Jersey City
Newark
Paterson
Albuquerque
Charlotte
Raleigh
Bismarck
Cleveland
Columbus
Dayton
Hamilton
Toledo
Youngs town
Tulsa
Portland
Allen town
Altoonaa
Erie
Johnstown
Pittsburgh
Scran ton
York
Providence
Charleston
Columbia3
Sioux Falls
Chattanooga
Knoxville
Beaumont
Dallas
Galveston
Houston
San Antonio
of Particulate
110
73
24
46
200
2.4
33
16
53
33
33
46
51
15
290
180
5.8
110
70
78
83
100
190
13
96
26
280
70
58
16
33
31
24
68
120
31
120
210
13
6.1
3.4
12
5.8
ug BaP/g of
Benzene Soluble
Fraction
1,500
1,600
230
540
1,800
51
460
160
600
410
440
500
610
460
2,100
1,300
130
1,200
930
760
600
1,200
2,000
180
730
440
1,400
1,000
660
200
360
510
240
530
750
480
1,000
1,900
200
160
50
210
110
|jg BaP/
m3 air
.012
.014
.0012
.0065
.054
.00011
.0035
.0014
.0060
.0055
.0060
.0045
.0063
.0063
.039
.014
.00044
.024
.0095
.0079
.014
.011
.028
.0010
.0080
.0034
.061
.0095
.016
.0051
.0061
.0056
.0029
.0056
.024
.0040
.031
.024
.00082
.0014
.00016
.0016
.00086
                                                             (continued)

-------
APPENDIX
                                                                    112
                           TABLE 7 (Continued)

                  BENZO(A)PYRENE CONCENTRATIONS IN URBAN
               SAMPLING SITES FOR JANUARY THROUGH MARCH 1959
     State
City
of
lag BaP/g
Particulate
                                                   ug BaP/g of
                                                 Benzene Soluble |j.g Bap/
Fraction
                                                                 rrr
                                                                    air
Utah
Vermont
Virginia


Washington
West Virginia

Wisconsin

Wyoming
Salt Lake City
Burlington
Norfolk
Richmond
Roanoke
Seattle
Charleston
Wheeling
Madison
Milwaukee
Cheyenne
5.4
28
59
410
160
81
40
140
80
60
36
54
39
580
1,900
1,100
790
900
1,600
830
730
340
.00052
.0010
.0084
.045
.018
.0090
.014
.021
.0049
.0085
.0012
         aln  respect to the cities with benzo(a)pyrene levels greater
than  11  |ag/l,000 m3 of air, five cities had a concentration of particu-
lates in the air 1.5 to 2 times higher than in the corresponding
January  to March period in 1958.

          The concentration of particulates in the air for this city in
January  to March 1959 was half that found in the corresponding period
of 1958.

-------
                                      TABLE 8

    POLYNUCLEAR HYDROCARBON CONTENT OF PARTICULATE MATTER  FOR SELECTED CITIES
213
City
Winter 1959
( |~ig/g benzene soluble
fraction)
Atlanta
Birmingham
Detroit
Los Angeles
Nashville
New Orleans
San Francisco
Seattle
Sioux Falls
South Bend
Wheeling
Youngs town
Summer 1958
( |-ig/g benzene soluble
fraction)
Atlanta
Birmingham
Cincinnati
Detroit
Los Angeles
Nashville
New Orleans
Philadelphia
San Francisco
Month



Feb.
Feb.
Feb.
Feb.
Jan.
Feb.
Jan.
Jan. -Mar .
Jan .-Mar.
Jan . -Mar .
Jan . -Mar .
Jan . -Mar .



July
July
July
July
July
July
July
July
July
Compound*
BqhiP*



830
1090
2100
510
880
760
590
1200
1000
1500
1100
1600



510
950
600
1500
200
440
530
960
720
BaP



690
1500
2000
150
1300
430
180
790
480
2000
1600
2000



160
730
390
950
43
180
230
480
69
BeP



440
610
1500
230
710
670
230
—
—
1700
—
—



150
670
400
840
54
150
360
350
150
BkF



560
800
1300
160
790
410
130
710
330
1300
990
1300



130
520
350
770
39
130
210
350
67
P



560
1000
1600
170
1400
240
150
590
480
3900
1700
2400



73
240
170
440
23
75
39
310
25
Cor



400
210
410
330
240
280
380
1300
440
480
360
240



250
270
280
290
190
160
290
410
450
Per



100
330
390
44
230
84
27
210
99
370
200
530



40
240
93
270
29
27
45
110
12
Anth



48
130
130
4.5
92
11
8.1
91
27
190
110
240



20
29
6.5
61
2.4
7.7
12
17
6.1
Total



3628
5670
9430
1599
5642
2885
1695
4891
2856
11440
6060
8310



1333
3649
2290
5121
580.4
1170
1716
2987
1499
     *BghiP = Benzolg, h, ilperylene; BaP = Benzo(a)pyrene; BeP = Benzo(e)pyrene;  BkF
Benzo(k)fluoranthene; P = Pyrene; Cor = Coronene; Per = Perylene; Anth = Anthanthrene
                H
                U)

-------
APPENDIX
                                                                  114
                             TABLE 9

      SEASONAL EFFECT ON THE BENZO(A)PYRENE CONCENTRATIONS
                 OF VARIOUS URBAN ATMOSPHERES
Concentrations
(Ug/m3 of air)
Location
Atlanta, Ga.
Birmingham, Ala.
Cincinnati, Ohio
Detroit, Mich.
Location I
Location II
Location III
Los Angeles, Calif.
Nashville, Tenn.
New Orleans, La.
New York, N.Y.
Herald Square
Columbus Circle
Philadelphia, Pa.
San Francisco, Calif.
South Charleston, W. Va.
Year
1958
1960
1958
1960
1958
1960
1958
1961
1961
1963
1958
1958
1958
1960
1963-64
1964
1958
1958
1960
S ummer
(low)
0.0016
0.0009
0.006
0.003
0.002
0.0012
0.0034
0.0072
0.0036
0.0002
0.0004
0.0014
0.0020
0.0006
0.0005
0.0007
0.0025
0.0003
0.0006
Winter
(high)
0.015
0.014
0.074
0.062
0.026
0.018
0.031
0.017
0.0137
0.0018
0.013
0.055
0.006
0.007
0.0094
0.0026
0.019
0.0075
0.012

-------
APPENDIX
                                                                     115
                                 TABLE 10
       CONCENTRATIONS  OF BENZO (A)PYRENE IN THE AMBIENT AIR, 19664
                                 (iag/m3 )
Location
1st
Quar.
2nd
Quar.
3rd
Quar.
4th
Quar
Yearly
Avq
Alabama
  Birmingham
  Gadsden
  Mobile

Alaska
  Anchorage

Arizona
  Grand Canyon
,0163
.00297
.00239
,00392
,0163
,00297
,00239
,00392
,00536
,00176
,00671
,00063
,036
,00626
,01430
,00063
.01849
.00349
.00645
.00228
National Park*
Paradise Valley
Phoenix
Tucson
Arkansas
Little Rock
Montgomery County*
Texarkana
West Memphis
California
Bur bank
Humboldt County*
Los Angeles
Oakland
Pasadena
San Diego
San Francisco
Colorado
Denver
Montezuma County*
Connecticut
Hartford
New Haven
Delaware
Kent County*
Newark
Wilmington
.00028
.00000
.00135
.0005

.00072
.00036
.00027
.0009

.00211
.00026
.0024
.00306
.00171
.00117
.00108

.00104
.00006

.00248
.00324

.00074
.00072
.00162
.00028
.00000
.00135
.0005

.00072
.00036
.00027
.0009

.00211
.00026
.0024
.00306
.00171
.00117
.00108

.00104
.00006

.00248
.00324

.00074
.00072
.00162
.00036
.00104
.00266
.00041

.00126
.00012
.00086
.00054

.0005
.00032
.00086
.00122
.0005
.00041
.00095

.00225
.00010

.00135
.00239

.00046
.00135
.00158
.00022
.00023
.00140
.00095

.00198
.00042

.00207

.00513
.00056
.00257
.00356
.00339
.0041
.00122

.00486
.00006

.0027
.00504

.00038
.00108
.00378
.00029
.00032
.00169
.00059

.00117
.00032

.0011

.00246
.00035
.00206
.00273
.00183
.00171
.00108

.00230
.00007

.00225
.00348

.00058
.00097
.00215

-------
APPENDIX
116
                           TABLE 10 (Continued)



       CONCENTRATIONS  OF BENZO(A)PYRENE IN THE AMBIENT AIR,  1966
Location
District of Columbia
Washington
Georgia
Atlanta
Hawaii
Honolulu
Idaho
Boise
Butte County*
Illinois
Chicago
Indiana
East Chicago
Hammond
Indianapolis
Monroe State Forest*
Muncie
New Albany
Parke County*
South Bend
Terre Haute
Iowa
Davenport
Delaware County*
Des Moines
Dubuque
Kansas
Kansas City
Wichita
Kentucky
Ashland
Covington
Louisville
1st
Quar.

.00090

.00113

.00009

.00266
.00010

.00266

.00752
.00572
.011
.00044
.00207
.00293
.00134
.00117
.00689

.00509
.00074
.00279
.00509

.00108
.00068
.0122
.00288
.00257
2nd
Quar.

.00090

.00113

.00009

.00266
.00010

.00266

.00752
.00572
.011
.00044
.00207
.00293
.00134
.00117
.00689

.00509
.00074
.00279
.00509

.00108
.00068
.0122
.00288
.00257
3rd
Quar.

.00068

.00144

.00023

.00167
.00010

.00306

.00707
.00248
.00707
.00048
.00257
.00099
.00026
.00284
.00806

.00158

.00216
.00171

.00149
.00032
.00657
.00225
.00167
4th
Quar.

.00693

.00185

.00023

.00680


.00495

.005
.00149
.0124
.00050
.00275
.0147
.00054
.00378


.00095
.00018
.00243
.00225

.00108
.00144
.0109
.00432
.00324
Yearly
Avq

.00235

.00139

.00016

.00345


.00333

.00678
.00385
.01037
.00047
.00237
.00539
.00087
.00224


.00318

.00254
.00354

.00118
.00078
.01047
.00308
.00251
                                                              (continued)

-------
APPENDIX
117
                           TABLE 10 (Continued)

       CONCENTRATIONS OF BENZO(A)PYRENE IN THE AMBIENT AIR, 1966
                                 (ug/m3 )
Location
Louisiana
New Orleans
Maine
Acadia National Park*
Portland
Maryland
Baltimore
Calvert County*
Michigan
Detroit
Minnesota
Duluth
Minneapolis
Moorhead
St. Paul
Mississippi
Jackson
Jackson County*
Missouri
Kansas City
St. Louis
Shannon County*
Montana
Helena
Glacier National
Park*
Nebraska
Omaha
Thomas County*
Nevada
Las Vegas
White Pine County*
1st
Quar.
.00203
.00012
.00180
.0023
.001
.00459
.00149
.00108
.00045
.00176
.00086
.0002
.00234
.00698
.00008
.00234
.00024
.00392
.00012
.00099
.00004
2nd
Quar.
.00203
.00012
.00180
.0023
.001
.00459
.00149
.00108
.00045
.00176
.00086
.0002
.00234
.00698
.00008
.00234
.00024
.00392
.00012
.00099
.00004
3rd
Quar.
.00158
.0004
.00171
.00171
.00024
.0027
.00396
.00099
.00063
.00126
.00099
.00006
.00122
.00167
.00004
.00018
.00046
.00131
.00008
.00041
.00002
4th
Quar.
.00347
.00008
.00177
.00473
.00022
.00702
.00198
.00342
.0014
.00243
.00239
.00014
.00108
.00563
.00008
.00077
.00028
.00167
.00026
.00266
.00008
Yearly
Avq
.00228
.00018
.00177
.00276
.00062
.00473
.00223
.00164
.00073
.00180
.00128
.00015
.00175
.00532
.00007
.00141
.00031
.00271
.00015
.00126
.00005
                                                             (continued)

-------
APPENDIX
118
                            TABLE 10 (Continued)



       CONCENTRATIONS  OF BENZO(A)PYRENE IN THE AMBIENT AIR,  1966
Location
New Hampshire
Concord
Coos County*
New Jersey
Camden
Glassboro
Jersey City
Marlton
Newark
Perth Amboy
Trenton
New Mexico
Albuquerque
Rio Arriba County*
New York
New York City
Cape Vincent*
North Carolina
Charlotte
Cape Hatteras*
Ohio
Akron
Cincinnati
Cleveland
Columbus
Dayton
Toledo
Young stown
Oklahoma
Cherokee County*
Oklahoma City
Tulsa
Oregon
Curry County*
Portland
1st
Quar.

.00054
.00024

.0027
.00081
.00509
.0014
.00225
.00189
.00216

.0023
.00018

.00491
.0002

.00509
.00016

.00468
.00432
.00356
.00405
.00275
.00221
.00567

.00024
.00176
.00086

.0001
.00293
2nd
Quar.

.00054
.00024

.0027
.00081
.00509
.0014
.00225
.00189
.00216

.0023
.00018

.00491
.0002

.00509
.00016

.00468
.00432
.00356
.00405
.00275
.00221
.00567

.00024
.00176
.00086

.0001
.00293
3rd
Quar.

.00041
.00016

.00207
.00018
.0023
.00117
.00054
.00189
.00063

.00036
.0001

.00158
.00032

.00077
.00018

.00153
.00113
.00207
.00162
.00203
.0009
.00599

.00008
.00108
.00041

.0001
.00104
4th
Quar.

.00077
.00028

.00441
.00113
.00419
.00095
.0032
.00266
.00369

.00311
.00028

.005
.00016

.0119
.00038

.00545
.00441
.00338
.00194
.00329
.00185
.0118

.00022
.00122
.00068

.00012
.00626
Yearly
Avq

.00057
.00023

.00297
.00073
.00417
.00123
.00206
.00208
.00216

.00202
.00019

.0041
.00022

.00571
.00022

.00409
.00355
.00314
.00292
.00271
.00179
.00728

.0002
.00146
.0007

.00011
.00329

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APPENDIX
119
                            TABLE 10 (Continued)

       CONCENTRATIONS OP BENZO (A)PYRENE IN THE AMBIENT AIR, 1966
                                  (ug/m3)
Location
P enn sy 1 van i a
Clarion County*
Lancaster
Philadelphia
Pittsburgh
Reading
Warm in star
Westchester
Puerto Rico
Bayamon
Guayanilla
Ponce
San Juan
Rhode Island
Providence
Washington County*
South Carolina
Columbia
Greenville
Rich land County*
South Dakota
Black Hills Forest*
Sioux Falls
Tennessee
Chattanooga
Memphis
Nashville
Texas
Dallas
Houston
Matagora County*
Pasadena
San Antonio
1st
Quar.

.0017
.00176
.0041
.00608
.00207
.0009
.0009

.0005
.00027
.0005
.00149

.00284
.00028

.00239
.005
.00212

.00012
.00095

.00621
.00081
.00545

.00113
.00099
.00028

.00041
2nd
Quar.

.0017
.00176
.0041
.00608
.00207
.0009
.0009

.0005
.00027
.0005
.00149

.00284
.00028

.00239
.005
.00212

.00012
.00095

.00621
.00081
.00545

.00113
.00099
.00028

.00041
3rd
Quar.

.00066
.00032
.00158
.00527
.00063
.00018


.00014
.00018
.0005
.00041

.00144
.00022

.00045
.00081
.00018

.00016
.00054

.00167
.00077
.00117

.00131
.00063
.00026

.00032
4th
Quar.

.00172
.00522
.00536
.00203
.00437
.00176


.00074
.0014
.00095
.0009

.00743
.00032


. 009 18
.00046

.00018
.00077

.0193
.00423
.00981

.00189
.00095
. 00044
.00122
.00104
Yearly
Avq

.00145
.00227
.00379
.00487
.00229
.00094


.00047
.00053
.00061
.00107

.00364
.00028


.005
.00122

.00015
.0008

.00835
.00166
.00541

.00137
.00089
.00032

.00055

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APPENDIX
                                                                   120
                           TABLE 10 (Continued)




      CONCENTRATIONS OF BENZO (A) PYRENE IN THE AMBIENT AIR, 1966
Location
Utah
Ogden
Salt Lake City
Vermont
Burlington
Orange County*
Virginia
Danville
Norfolk
Shenandoah Park*
Wisconsin
Door County*
Milwaukee
Wyoming
Cheyenne
Yellowstone Park*
1st
Quar .
.00014
.00018
.00113
.00136
.0014
.00216
.00132
.00028
.00617
.00054
.00006
2nd
Quar.
.00014
.00018
.00113
.00136
.0014
.00216
.00132
.00028
.00617
.00054
.00006
3rd
Quar.
.00041
.00005
.00050
.00036
.00086
.00041
.00032
.00131
.00037
.00002
4th
Quar.
.00149
.00455
.00041
.00068
.00905
.00662
.00068
.00275
.00063
.00008
Yearly
Avq
.00055
.00124
.00079
.00094
.00318
.00284
.00091
.00410
.00052
.00006
         *Indicates nonurban areas.

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