STRATIGRAPHIC EVIDENCE OF HUMAN DISTURBANCE
         IN SOME CHESAPEAKE BAY TRIBUTARIES
                    Final Report
                         by
          Grace S. Brush and Frank W, Davis
Department of Geography and Environmental Engineering
            The Johns Hopkins University
             Baltimore, Maryland  21218
           Grant Numbers  R 805 962 and
                          806 680 01
                   Project Officer
                  Dr. David Flemer
               Chesapeake Bay Program
        U. S. Environmental Protection Agency
                  2083 West Street
              Annapolis, Maryland 21401

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     STRATIGRAPHIC EVIDENCE OF HUMAN DISTURBANCE
         IN SOME CHESAPEAKE BAY TRIBUTARIES
                    Final Report
                         by
          Grace S. Brush and Frank W. Davis
Department of Geography and Environmental Engineering
            The Johns Hopkins University
             Baltimore> Maryland  21218  iQO-/
           Grant Numbers  R 805 962 and
                          806 680 01
                   Project Officer
                  Dr. David Flemer
               Chesapeake Bay Program
        U. S. Environmental Protection Agency
                  2083 West Street
              Annapolis, Maryland 21401

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                                  CONTENTS
Summary	.	   i
Figures	iii
Acknowledgements	  iv

     1.  Introduction	   1
     2.  Methods	   4
     3.  Results	   7
              Furnace Bay	   7
              Patuxent River	  10
              Ware River	  19

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                                   SUMMARY
     The stratigraphic record indicates that for centuries prior to European
settlement, diatom and macrophyte populations were fairly uniform in the
Chesapeake Bay estuaries studied.  Waters in tributaries draining Piedmont
watersheds underlain for the most part by thick saprolites appear to have
been clear with sufficient nutrients to support diverse and abundant sub-
merged macrophyte .and benthic diatom populations.   In the tributary draining
sandy Coastal Plain sediments, habitats were extremely oligotrophic capable
only of supporting a very sparse diatom population.   After initial defores-
tation, macrophytes and diatoms showed significant changes, many of which
were coincident with historically documented changes in land and water use.
Today, forests comprise about 40% of the land; sediment yield is 5 to 10     :
times and nutrient loading 2 to 6 times greater in agricultural than in
forested watersheds (Lystrom et al. 1978) .   The rate of application of high-
nitrate chemical fertilizers, applied to cultivated land since the roid-19th
century, has quadrupled since 1900.  Since the mid-20th century, sewage has
increased phosphorus-loading of some estuarine tributaries by 5 to 15 times. •

     At oligohaline locations, strong correlations between macrophyte seed
and diatom stratigraphy are coincident with land clearance and nutrient load-
ing.  At Jug Bay, in the Upper Patuxent River, the appearance of Potamogeton '.
epihydrus and Zannichellia palustris along with an increase in flux of peri-
phytic diatoms occurred at about the time fertilizers were added to the
watershed.  At Furnace Bay, in the Upper Chesapeake Bay, low diatom fluxes
accompanied by the first major discontinuities in the macrophyte record fol-
lowed early European land clearance.  A large increase in diatom flux, domi-
nated by the eutrophic planktonic Fragilaria crotonensis, around 1930, was
associated with the first appearance of the eutrophic adventive macrophyte
Myriophyllum spicatum.  These population changes were coincident with the
initial discharge of sewage effluent into Furnace Bay.  At both locations,
the proportion of planktonic diatoms has increased in recent years.  At the
same time, seed stratigraphy (particularly at Furnace Bay) and field surveys
have documented severe declines in macrophyte populations.  At mesohaline
locations, the stratigraphic record shows that the effect of human disturb-  :
ance is much less obvious and suggests that the greatest alteration of estua-
rine habitats is mainly a result of local disturbance.

     Causal links between eutrophication and epiphytic diatom and macrophyte (
populations have been studied in lakes by Phillips et al. (1978) and summa-  ;
rized for Chesapeake Bay by Valentine and Stevenson (1981).  Based on obser- •
vations in lakes in England, Phillips et al. (1978)  postulated that increased
nutrient loading stimulates the growth of epiphytic and filamentous algae

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which normally are  suppressed by macrophyte-secreted chemicals and law
nutrients.  The accelerated algal growth decreases the light available to
the macrophytes.  If nutrient levels continue to increase, phytoplankton
growth also increases thus further shading the macrophytes and resulting
eventually in the elimination of both the macrophytes and associated epi-
phytes.  In the sediments of Jug Bay and Furnace Bay, increasing flux of
epiphytic diatoms with  fertilizers, large increases in both epiphytic and
planktonic diatoms  with sewage, and a rise in planktonic species in recent
years concurrent with the decline of macrophytes support the hypothesis of
Phillips et al.  (1978).  The epiphytic diatoms do not decline as sharply as
might be expected especially as the macrophytes were essentially eliminated
from both areas.  However, only a few epiphytic diatoms occur only on macro-
phytes; many can also use filamentous algae and nearby emergent vegetation
as a substrate.  It should be noted also that factors other than eutrophica-
tion, including a catastrophic flood in 1972 (Bayley et al. 1978) and re-
gional increases in herbicide use since 1970 (Kemp et al. 1981) also could
have played a role  in the recent decline of macrophytes in the Chesapeake
Bay.
                                     ii

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          .                         FIGURES

Number.

   1.      Map of Chesapeake Bay showing locations of areas cored:
           1. Furnace Bay, 2. Jug Bay, 3. Eagle Harbor, 4. St.
           Leonard Creek, 5. Ware River - midstream, 6. Ware River
           downstream	        2

   2.      Pollen profile at Furnace Bay.  TP - total pollen; O:R
           - the ratio of oak to ragweed pollen.  Similar abbrevi-
           ations are used in all pollen profiles..	        8

   3.      Diatom and macrophyte profile at Furnace Bay.  Ecolog-
           ical groupings based on Patrick and Reimer  (1966,1975);
           Hustedt  (reprinted 1977); Lowe  (1974); Schoeman (1973);
           and Davis and Brinson (1980).  In all figures, profiles
           of.eutrophic diatoms are colored black, and fluxes
           < IxlO1* are shown as a black dot	    •    9

   4.      Pollen profile at Jug Bay in the upper Patuxent River...        11

   5.      Diatom and macrophyte profile at Jug Bay, upper Patux-
           ent River	        13

   6.      Pollen profile at Eagle Harbor in the middle Patuxent
           River	        15

   7.      Diatom profile at Eagle Harbor in the middle Patuxent
           River	        16

   8.      Pollen profile at St. Leonard's Creek in the lower
           Patuxent River	        17

   9.      Diatom and macrophyte profile at St. Leonard's Creek
           in the lower Patuxent River	        18

  10.      Pollen profile at the midstream location in Ware River..        20

  11.      Diatom profile at the midstream in Ware River	        21

  12.      Pollen profile at the downstream location in Ware River.        23

  13.      Diatom profile at downstream in Ware River	        24
                                      111

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                             ACKNOWLEDGEMENTS
     We express our appreciation to the following individuals:  to R.
Summers for helping in core collecting; J. Stasz and S.  Rumer for diatom
counts; C. Stenger for assisting with pollen counts and  historical research;
R. Patrick and F. Uhler for lending their expertise in providing identifi-
cations and ecological requirements of diatom and macrophyte species; W.
Boynton, J. P. Bradbury, O. Bricker, D. Flemer, K. Mountford, J. Sanders
and M. G. Wolman for reviewing the manuscript.
                                    IV

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                                  SECTION 1

                                INTRODUCTION
     Organisms and parts of organisms preserved in estuarine sediments provide
a quantitative record of changes in populations that can be compared with the
historical and stratigraphic records of environmental change.  Stratigraphic
records of biological populations in recent sediments are particularly valu-
able for evaluating effects of environmental change because the historical
records are often meagre, seldom quantitative, and do not allow comparisons
between pre-historic and historic conditions.  We investigated the history of
diatom and submerged macrophyte populations preserved in sediments deposited
over several centuries in 3 areas of Chesapeake Bay in order to assess the
effect of human disturbance on estuarine habitats.  The disturbances investi-
gated, which have altered parts of estuaries, include sediment loading from
land clearance, nutrient loading from fertilization of the cultivated land-
scape and the discharge of sewage effluent into estuarine waters, thermal
loading from cooling water discharged by a power plant, and toxic loading
from herbicide application to the farmed land and chlorination of both sewage
treatment and power plants.

      Fifteen sediment cores, 1 to 2 m long and 5.4 cm in diameter were col-
lected with a piston corer at 6 locations in the upper, middle and lower
Chesapeake Bay (Figure 1).

     Furnace Bay is a shallow (1-2 m), oligohaline (0.5-1.0 °/oo) erobayment
at the head of Chesapeake Bay.  It drains a watershed of 68 km2, underlain by
unconsolidated Cretaceous sediments and saprolite derived from Piedmont crys-
talline rocks.  Four ..cores were taken at this locality.  Locally, major human
activities since European settlement of the watershed include agriculture
since 1730, fertilization of cultivated land since 'V 1845, charcoal produc-
tion from 1720 to 1900, sewage disposal.presently 1 mgd (U. S. Army Corps of
Engineers 1977) since 1918, sand and gravel mining since 1950 and the use of
herbicides since the mid-19601s.

     The Patuxent River at approximately the mid-section of Chesapeake Bay,
is 285 km long and drains an area of 'V 2400 km2 underlain by Piedmont sapro-
lite and unconsolidated Coastal Plain sediments.  Nine cores were taken at 3
locations.  Jug Bay, 84 km upstream from the mouth, is a shallow, oligohaline
'(0.1-1.3 °/oo).embayment, always turbid and eutrophic.  Eagle Harbor at river
km 44 in the mesohaline stretch  (3-9 °/oo salinity) is located at the end of  ;
the discharge canal of Chalk Point Power Plant  (^ 900 MW) and is within the
turbidity maximum (Roberts and Pierce 1976).  St. Leonard Creek at river km
15, is the most saline site (9-16 °/oo) and is outside both the turbidity  —;

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                 ESAPEAKE BAY
            i'i  0  ^  0 'JiL:ciL MILES
Figure 1.  Map of Chesapeake Bay showing locations of areas cored:
           1. Furnace  Bay,  2.  Jug Bay, 3. Eagle Harbor, 4. St.
           Leonard  Creek,  5. Ware River - midstream, 6 Ware River
           downstream.
                                 2

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maximum and the thermal plume of the power plant.  The entire Patuxent water-
shed was farmed primarily for tobacco from 1650 to 1840 and also for grain
from 1840 to the present (Craven 1925).  Chemical fertilizers have been ap-
plied to cultivated land since the mid-to-late 1800's and herbicides since
the mid-19601s.  Intense urbanization of the upper watershed since the early
1960's has resulted in a present discharge of 35 mgd of effluent from 29 sew-
age treatment plants into the Upper Patuxent River (U. S. Army Corps of Engi-
neers 1977).  During the past 20 years, nutrient and suspended solid concen-
trations have increased throughout the estuary, with highest concentrations
in the upper estuary, lower in the vicinity of Eagle Harbor and lowest at the
mouth (Roberts and Pierce 1976).  The Chalk Point Power Plant went into
operation in 1965; since that time water temperature within the thermal plume
has been raised ah average of 5 to 8°C.  However, the temperature increase   ;
affects primarily surface water, except at the discharge canal (1.5 km long),
where mixing ensures a more or less uniform vertical temperature profile
(Carter 1968). ~  •

     The Ware River in the Lower Chesapeake Bay is 12 km long.  Its rural
watershed of 58 km2 is underlain by sandy Coastal Plain sediments.  Salini-
ties range from 5-13 °/oo at the head to 17-20 °/oo at the mouth.  Two cores
were analyzed from 2 locations.  The watershed was initially farmed for
tobacco and since the mid-19th century primarily for grain.  Chemical ferti-
lizers have been used since the mid-to-late 19th century and herbicides since
the mid-1960's.  A secondary sewage treatment plant with an average discharge
of 0.13 mgd. was built near the head of the estuary in 1936 (U. S. Army Corps
of Engineers 1977); its effects are restricted to a small area immediately
downstream from the plant (VIMS, unpublished data).

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                                  SECTION 2

                                   METHODS
     Cores were extruded and  split  lengthwise.  Splits analyzed for pollen
and diatoms were divided into 1 and 2 cm intervals.  The procedure for ex-
tracting pollen from the sediments  follows that outlined by Faegri and
Iversen (1964).   Diatoms were extracted using a modified version of the
method  described by Funkhouser and  Evitt  (1959).  Pollen and diatoms were
each extracted from 1 cm3 of  sediment from each interval analyzed.  Identifi-
cations and counts  were made  from several aliquots from each sample.  Diatom
fluxes  (number of frustules deposited cm"  y~  were calculated by multiplying
the total number of frustules per cm3 of sediment by the appropriate sedimen-
tation  rate  (cm y"1).   Because of the large number of species enumerated from
these cores,  most of which occurred < 0.5%, we included in our analyses only
those species that  comprised  >. 3% of the population at a level.  Complete
vertical distributions of fluxes  of all species comprising >. 3% of the popu-
lation  arranged into major ecological groups were plotted against time axes
 (Figures 3, 5, 7, 9, 11 and 13).

     Splits analyzed for seeds were divided into 4 cm intervals  (40-50 ml).
Seeds were extracted from all samples by first disaggregating the sediment in
dilute  nitric acid  (Godwin 1956)  and then washing the sample through 20-mesh
and  60-mesh sieves  (0.8 mm and 0.25 mm apertures).  All macrophyte seeds were
identified and counted, and seed  identifications confirmed using the seed
reference collection at the Patuxent Migratory Bird Habitat Research Labora-
. tory in Laurel, Maryland.  The presence of seeds from continuous samples in
several cores 50-100 m apart  at a location was plotted against the time axis.

     We measured the effect of environmental change on diatom populations by
analyzing one core  at each location.  This was considered representative of
the  local population because  calculated settling velocities of diatom  frus-
tules are  in  the range of 15  m/day  and observed settling velocities range
from 1  to  30  m/day  (Smayda 1971).  These rates indicate that diatoms are not
transported  far in  shallow estuaries.  A  comparison of pollen distributions
in surface  sediments of the Potomac estuary with tree and shrub  distributions
in a broad band along the estuary show that the local patchiness of the vege-
tation  is  erased in the pollen distributions due to estuarine transport, but
the  regional  gradients are maintained  (Brush and DeFries 1981).  Pollen
grains  are  similar  in size to diatom frustules, but they have a  lower  speci-
fic  gravity  and hence are likely  to be transported farther than  diatoms be-
fore being deposited.  Thus,  we expect that estuarine transport  erases local
variations  in diatom distributions  but does not result in regional mixing

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      We  measured the  effect  of  habitat alteration on  submerged macrophytes
 by  analyzing  several  cores at a location, because unlike diatoms which range
 from ^ 20-150P,  the larger macrophyte seeds  (1-2 mm)  are not  transported far
 from the original grass  beds thus  resulting  in  localized and  patchy  distri-
 butions  of  seeds in the  sediments  (Davis  1982).

      Selective preservation  of  macrophyte seeds (Stark  1971)  and dissolution
 of  diatom frustules  (Parker  et  al.  1977;  Hecky  and Kilham  1973) have been
 documented  in some sedimentary  environments.  We selected  for study  cores
 with relatively  intact stratigraphy and good fossil, preservation.  Except
 for poor preservation of Potamogeton fruits,  seeds were well  preserved at 3
 locations.  Diatoms were consistently well preserved  in all cores  analyzed.

      In order to construct chronologic profiles of diatom  fluxes and seed    :
 occurrences,  sediments in the cores were  dated  based  on changes in the verti-
 cal pollen  profiles reflecting  historically  dated changes  in  regional vegeta-
 tion caused by deforestation and disease  (Davis 1968; Anderson 1974).  In all
 cores, initial European  settlement is recorded  in the sedimentary  record by
 an increase in percent of ragweed  pollen  and a  corresponding  decrease in the
 rates of oak  to  ragweed. During modern intensive agriculture, the percent
 of land cleared  for agriculture increased from  < 20 to  40  to  60.   This is
 reflected in  the pollen  profiles by a dramatic  increase in ragweed generally
 to between  20 to 30%  of  total pollen.  The ratio of oak to ragweed is almost
 always < 1.  Dates assigned  to  the stratigraphic horizons  representing these
 2 phases of land clearance  (initial or early European settlement and inten-
 sive agriculture) are different for different watersheds  (Figures  2, 4, 6, 8,
 10 and 12) .  The demise  of American chestnut about  1930 is reflected in pol-
 len profiles  from Furnace Bay either by the  absence of  chestnut or a decrease
 to < 1%.

      Proportions of ragweed  and chestnut  of  total pollen and  the ratio of oak
 to ragweed pollen were plotted  against depth for each core.   Time  lines were
 drawn where the  changes  described  above occurred in the pollen profiles.
 Sedimentation rates were averaged  between dated horizons by dividing the
 length of the core between dated horizons by the time between the  horizons.

      Uncertainties in sedimentation rates, resulting  from  the size of the
 sampling intervals, distance between sampled intervals  and the error in-
 volved in assigning a precise date for an event which may  have occurred over
 several years were calculated using the first order propagation of error
 (Benjamin and Cornell 1970  ; Brush et al. 1982) and are recorded in  the pol-
 len profiles  (Figures 2, 4,  6,  8,  10 and  12).

      Although it is necessary to use average sedimentation rates between     j
 dated horizons,  we recognize that  sediment accumulation was probably not uni-:
 form at all localities over  the time periods studied.  This  can  also lead to
 error in the  calculation of  population fluxes.  As  of now, we have no means  ]
 for estimating this error.                                                  •  j

      We have  assumed  a 0.1  cm y~  sedimentation rate  for the  period  of time  '
" prior to European settlement.  This rate  is  based on  an average  of rates   	

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ranging between 0.05 and 0.18 cm y~l derived from ltfC dated sediments from
some Chesapeake Bay tributaries.  The 14C dated sediments were selected from
cores where sedimentation is believed to have been continuous since the time
llfC dated sediment was deposited (Kraft and Brush unpublished data; Wilke
et al. 1981).

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                                  SECTION  3

                                   RESULTS
 FURNACE BAY

      The pollen profile  (Figure  2) shows the early settlement  Cv  1730) hori-
 zon at 92 cm where percent  ragweed increases from 4 to 13 and  the ratio  of
 oak to ragweed decreases  from 8  to 2; the  intensive agricultural  horizon
 C^ 1780)  at  83 cm where percent  ragweed increases to > 20 and  the oak-rag-
 weed ratio becomes <  1; and the  demise of  chestnut (^ 1930) horizon at 28 cm
 where percent chestnut pollen decreases to < 1.  Based on these horizons,
 average rates of sediment accumulation were 0.16 ± 0.04 cm y"1 during early
 settlement,  0.37 ± 0.02 cm  y"1 during intensive agriculture when  percent
 land cleared reached  60,  and 0.58 ±  0.06 cm y"1 from 1930 to 1978, when  the
 cores were collected.

     Out of a total of 246 diatom species identified and enumerated from  this
 core, 23 occurred in  percentages >_ 3  (Figure 3).  Prior to European settle-
 ment, an essentially  periphytic  flora was  dominated by Cocconeis  placentula.
 Some of the  species present at that  time,  such as Gomphonema parvulum,
 Navicula peregrina and Synedra pulchella are often found today in high-
 nutrient waters (Lowe 1974;  Schoemari 1973).  Planktonic species were extreme-
 ly rare except for Synedra  pulchella which can be either periphytic or plank-
 tonic.  As Europeans  cleared the land for  agriculture, sediment accumulation
 increased at Furnace  Bay.   Total diatom flux was reduced to one-half its
 original size.  Most  of the original species were still present following
 early land clearance  except Gomphonema parvulum and Synedra pulchella.   Both
 of these species disappeared almost  completely during this period.  Epithemia
 adnata minor was also greatly reduced.  The planktonic Synedra rumpens ap-
 peared and was present more or less  continuously from that time on. Navicula
 rhyncocephala alsa made its first appearance following early settlement.
 Coincident with the addition of  chemical fertilizers to the cultivated land,
 around the middle 19th century  (Davis 1982), total diatom flux increased.
 This increase was accompanied by an  increase in many species existing at
 that time.  Cocconeis placentula remained  dominant.  Some new  species, such
 as Achnanthes minutissima and the pollution-tolerant Navicula  cryptocephala
 (Lowe 1974)  appeared.  Gomphonema parvulum which favors high-nutrient water
 reappeared.   Early in this  century and coincident with the discharge of  sew-
 age effluent into Furnace Bay, diatom flux increased dramatically from ^ 1
;to > 5 million.  The  planktonic  Fragilaria crotonensis, considered an indica-
 tor of eutrophication (Lowe 1974; Brugam 1978) comprised 30% of the flora at
 that time.  Kilham  (1982) has shown  experimentally that this species is  a

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00
              Depth  Sediment
              (cm)
               40-
              80-
             130
              TP
             (no/g
             sed)
Ragweed   0:R   Chest-    Dated
                 nut     Horizon
Dark

Gray

Silt

and

Clay
                   Light gray

                   silt and

                     clay
                                                                        •1978  -1
                                                                        •1930  n
                             1x10
Sedimentation
    Rate
  (cm y-1)


0.58±0.06
                                                                                 0.37+0.02
                                                                                 0.16±0.04
                                                           assumed
                                                             0.10
                       Figure 2.  'Pollen profile at Furnace Bay.   TP - total
                                 pollen;  0:R - the ratio of oak  to ragweed
                                 pollen.   Similar abbreviations  are used in
                                 all profiles.

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                               DIATOMS

1
PERIPHYTIC/
PLANKTONIC
1 1 1
PLANK-
TONIC
1 1
                                                                                 HACROPHYTES
1400

                                                             •0 -H -O
                                                             I 8 I

                                                               kT
                                            si
                       All scales 1x10*
                  No. frustules deposited cm"2 y"1
                                                                      .1   a
                                                                               	Increasing    >
                                                                               turbidity tolerance
                                                                              5"t  CJ  -H
                                                                              -I  tJ  -1
                                                                                      u
                                                                                 • seeds present
                                                                                 (data from 4 cores)
       Figure 3.
Diatom and macrophyte profile at Furnace Bay.  Ecological group-
ings  based on Patrick and Reimer (1966,1975), Hustedt (reprinted
1977),  Lowe  (1974),  Schoeman (1973) and  Davis and  Brinson  (1980).
In all  figures, profiles of  eutrophic diatoms are  colored black,
and fluxes < 1x10**  are shown as a black  dot.

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good competitor for phosphorous, present in large quantities in sewage.  The
periphyte, Cymbella lunata present in small numbers but continuously until
the early 19th century, when it disappeared, reappeared in large numbers in
the early 20th century.  The eutrophic periphyte Synedra pulchella also re-
appeared at this time and Epithemia adnata minor disappeared and did not
return.  In the early 1950's, Fragilaria crotonensis crashed, contributing
to a decrease in total flux.  It was succeeded by species of Coscinodiscus
which can be either periphytic or planktonic and by the planktonic Melosira
granulata.  Among the eutrophic periphytes, Navicula peregrina disappeared
and Gomphonema angustatum appeared.

     Among the submerged macrophytes, Vallisneria americana, Najas gracillima
and Elodea canadensis occurred continuously prior to European settlement,
while Najas flexilis and N. guadalupensis were present most of the time.
During early settlement  (1730-1780), Najas flexilis and Elodea canadensis be-
came sporadic.  After 1780, as sediment accumulation doubled, Najas gracil-
lima disappeared and until 1930 all of the species except Vallisneria ameri-
cana, considered the most turbidity-tolerant of the macrophyte species  (Davis
and Brunson 1980), occurred infrequently.  The infrequency of occurrence was
particularly evident for Najas guadalupensis and Elodea canadensis.  Najas
flexilis occurred continuously from 1930 to the early 1970's.  Myriophyllum
spicatum, introduced into Eastern North America in the late 19th century,
first appeared in Furnace Bay between 1930 and 1940, reappeared after 1950,
and dominated aquatic macrophyte communities in this area between 1960 and
1967  (Bayley et al. 1978).  The species thrives in alkaline eutrophic lakes
(Seddon 1967; Lind and Cottam 1969) and can grow in moderately low light
(Davis and Brinson 1980; Wentz and Stuckey 1971) in a broad range of salini-
ties  (Rawls 1964).  After 1972, all native species of macrophytes disappeared
from the seed record, including Vallisneria americana which was present con-
tinuously up~to this time.                .

     The stratigraphic record of diatoms and macrophytes suggests that prior
to deforestation and cultivation of the watershed for agriculture, Furnace
Bay was a shallow, clear-water embayment supporting an abundant and diverse
macrophyte and periphytic diatom population.  Following land clearance, the
waters became turbid with increased sedimentation and populations of both
macrophytes and diatoms were reduced.  With the introduction of fertilizers,
populations increased and included more eutrophic periphytes.and free-float-
ing diatoms.  After the introduction of sewage, the embayment was transformed
into a more highly eutrophic system supporting a much larger planktonic dia-
tom population, and eventually the macrophyte populations disappeared com-
pletely from the area.

PATUXENT RIVER

Jug Bay

     The pollen profile from the core at Jug Bay in the upper Patuxent  River
(Figure 4) shows the early settlement Cv> 1650) horizon at 99 cm, where per-
cent ragweed changes from 2 to > 5 and the ratio of oak to ragweed changes
from 20 to < 10.  The beginning of intensive agriculture about 1840 occurs
                                     10

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Depth  Sediment
(cm)
  40-i
  80
 118
Brown-

 Gray

 Silt

  and

 Clay
         Gray-
        Yellow
         Dark

         Gray-

        Brown
            TP
           (no/g
           sed)
Ragweed  0:R
  Dated   Sedimentation
 Horizon       Rate
            (cm y'1)
- 1980  -i
                                            •1840  n
                                    •1650  q
                                                     0.41+0.03
                                                     0.16±0.02
                                             assumed
                                               0.10
                 1x10*   5%          5.

    Figure  4.   Pollen  profile at tfug Bay in the
                upper Patuxent River.

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at 56 cin where percent ragweed increases to > 20 and the oak-ragweed ratio
is ^ 1.  Based on these horizons, the average rate of sediment accumulation
which was 0.16±0.02 cm y"1 during the era of tobacco agriculture more than
doubled to 0.4110.03 cm y"1 when at least 40% of the watershed was cleared
for farming.

     Twenty-three of the 190 diatom species enumerated from this core oc-
curred 2. 3%  (Figure 5).  Total diatom flux remained fairly uniform before and
after European settlement until the beginning of the 20th century when it
more than doubled.  The pre-settlement flora was a diverse assemblage con-
sisting mainly of periphytes and including Cyclotella memenghiniana and the
planktonic Melosira granulata angustissima.  Both of these species are con-
sidered eutrophic  (Schoeman 1973; Lowe 1974).  Prior to settlement eutrophic
periphytes were extremely rare.  With the onset of European agriculture, a
number of species appeared including the eutrophic periphyte Navicula rhyn-
cocephala (Lowe 1974) and some species disappeared including Cyclotella
memenghiniana and Melosira granulata angustissima, also eutrophic.  Coinci-
dent with the application of chemical fertilizers to the watershed, total
diatom flux  began to increase.  This increase was accompanied by increases
in species that prefer high nutrient waters, such as Gomphonema parvulum,
Navicula rhyncocephala and W. viridula, all periphytes.  Cocconeis placen-
tula, unimportant prior to the middle 19th century, became a dominant part
of the flora, as did Achnanthes lanceolata.  With the introduction of sewage
effluent into the Upper Patuxent River in the 1950"s, diatom flux tripled,
accompanied  by the appearance in large numbers of species which had not been
present prior to this time or were present in very small numbers such as
Cyclotella menenghiana, C. kutzingiana, O.K. planetophora, and Nitzschia
parvula.  Species of Cyclotella can occur as periphytes or plankton.  The
eutrophic planktonic Melosira granulata angustissima also reappeared at this
time, and the eutrophic periphyte Navicula rhyncocephala, was present in
large numbers.  At the same time, populations of previously important peri-
phytes, such as Cocconeis placentula, Eunotia pectinalis and Navicula radi-
osa tenella  were greatly reduced.

     Pre-settlement sediments contain seeds of 6 macrophyte species  (Figure
5), the most common of which were Najas guadalupensis, W. flexilisf and
Elodea canadensis.  The seed record of the 18th and early.19th centuries is
poorly preserved.  Najas flexilis dropped out of the record and Elodea cana-
densis became sporadic during this time interval.  By the mid-19th century,
at about the time  chemical fertilizers were applied to the cultivated water-
shed, Potamogeton  epihydrus, which grows in mesotrophic to mildly eutrophic
alkaline waters,  (Hellquist 1975) appeared along with Zannichellia palustris.
The latter  is a shallow-water pioneer species in Chesapeake Bay, that tole-
rates very  high nutrient levels  (Stevenson and Confer 1978).  Between 1940
and 1960, when diatom flux increased enormously, Potamogeton epihydrus dis-
appeared.   Field  surveys show that since the late 1960's beds of submerged
macrophytes  declined  greatly in  the Upper Patuxent River  (Stevenson and
Confer 1978) and  this  is reflected in the  sediments by a decrease in macro-
phyte seeds  since  1960  (Brush et al. 1981).

     The large increase in diatom flux, dominated by species of Cyclotella
                                      12

-------
                                                               DIATOMS
                                                                                                          MACROPHYTES


PERTPHYTIC
||
PLANK-
PERTPHYTIC-PLAHKTOMIC TON 7 1 1
Increasing
— pollution — t
tolerance
I || | | || 1 | 1
U)
          1600-
          1400-
                   1x10
                                                       All scales 1x10*
                                             Number of frustules deposited cm"S y"1
• Seeds Present
(data front 4 cores)
                         Figure  5.   Diatom and macrophyte profile at  Jug  Bay,  upper
                                       Patuxent River.

-------
 and the disappearance of submerged macrophytes are coincident with the period"
 of  intensive urbanization of the watershed and increasing discharge of sewage
 effluent into the river.

 Eagle Harbor

      The pollen profile (Figure 6) shows  an  early settlement  (^ 1650) horizon
 at  115 cm,  where percent ragweed changes  from <  1 to >  2 and the oak-ragweed
 ratio from > 35 to < 10.  The horizon  representing intensive clearing  Cv
 1840) occurs at 60 cm, where percent ragweed increases  to > 20 and the oak-
 ragweed ratio decreases to < 1. Sediment accumulated at a rate of 0.22±
 0.02 cm y"1 during early settlement  and like the upper  part of the river more
 than doubled to 0.51±0.06 cm y"1 as a greater proportion of land was fanned.

      Of the 129 diatom species identified, 23 were present at >. 3%  (Figure
 7).  There is little change in total diatom  flux throughout the entire core,
 except in the very top part, where it  doubles.   Nor are there any dramatic
.changes in -the species composition throughout the core.  Essentially the
 flora consists of a mixture of periphytes and species that can be either
 periphytic or planktonic and includes  eutrophic  species at all levels.   The
 increase in flux in the early to mid-1970"s  is due mostly to a relatively
 large increase in Cyclotella kutzingiana.  There are no changes in the diatom
 flora strictly coincident with the discharge of  cooling water in the river at
 this location (the core was taken at the  end of  the Chalk Point Power Plant
 discharge canal).

      Seed stratigraphy was not analyzed at this  location because of low  seed
 concentrations in the sediment.

 St  Leonard Greeks

      The pollen profile (Figure 8) from the  core in the downstream part  of
 the Patuxent River indicates that the  overall sedimentation rate was  lower
 than at either up-or midstream. The early settlement horizon is at 69 cm,
 where percent ragweed changes from 1 to > 5  and  the oak-ragweed ratio from
 30  to < 5.   The intensive land clearance  horizon is located at 48 cm, indi-
 cated by an increase in percent ragweed to > 10  and a < 2.decrease  in the
 oak-ragweed ratio.  Thus, sediment accumulated at an average  rate of  0.1±
 0.009 cm y"1 during early settlement and  was probably not very different from
 the pre-settlement rate.  After land clearance reached  ^40%, sediment accu-
 mulation tripled, averaging 0.3410.03  cm  y"1.

      Total diatom flux is fairly uniform  throughout the core  (Figure  9).  A  .
 total of 110 species were identified,  of  which 17 comprise ^  3% of  the total
 population.  The population consists of a mixture of periphytes and  species
 that can be either periphytic or planktonic  and  includes a few species that
 prefer high nutrient habitats, such  as Nitzschia amphibia, Cyclotella bo-
 danica, Meridion spp., and Synedra fasiculata.   The only major change  in
 species composition in the core was  the disappearance of the  eutrophic peri-
 phyte Rhopalodia gibberula  (Lowe  1974) with  the  onset of agriculture, and
 Cyclotella bodanica became sporadic  in the late  19th century.
                                      14

-------
Depth Sediment
(cm)
             TP   Ragweed  O:R
            (no/g
            sed)
 40-,
 80-
128'
Uniform

 Gray

 Silt

 and

 Clay
                1x10"
                  5%
Dated
Horizon
                                               1980   -i
Sedimentation
    Rate
  (cm y-1)
                                               1840   =i
                                               1650   n
                                                        0.51±0.06
                                                        0.2210.02
        Figure 6.  Pollen profile at Eagle Harbor in
                   the middle Patuxent River.

-------

PERIPHYTIC i PERIPHYTIC/PLWJKTON1C
r ii 1 r
	 !
W HI
g"*H N ffi
-i m « q
*d -H OlQU • q> E
*H'H iJ Id • -H 41 0 IQQ«££
•H T1 * « « *> cmmu B 3 o -
Id MO -H3 Id IQC'^Q) oi?Q)E
0 DiaioSfl 4J •SSpSa^S
0 Mum •H3E: -H*H O'HU ti! '^S1^ ^cq4J>H -Hit
B 4J ji -q
— a ° 8 " ' ,
1980 "
1950-




1900-




1800-
1600-
non -
o
•o
r
(U
u

1
r-4



1
2
0)
N
•H
r-l
3
QJ




T

14
V
fl
3
•o
s
n)
V
X

Pre-
Europoan

























1












s












k
U













) Q« >.£
Q o! q fe a a












s












t












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1






••

r
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I
1












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a












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<
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-I
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3s













 1x10'
      All scales Ixlfl'
No. frustulcs deposited cm  y
Figure 7.  Diatom profile at  Eagle Harbor in
            the middle  Patuxent River.

-------
Depth  Sediment    TP   Rag-  O:R
(cm)             (no/g  weed
                  sed)
  0-
 40-
 80-
112
      Gray sand
      silt and
        clay
Dk. gray
sand, silt
 and clay
  Yellow-
   Gray
   Silt
   and
   Clay
               1x10
                                 Dated    Sedimentation
                                 Horizon      Rate
                                             (cm y"1)
                                     *	 1980 -i
                                          1840 S
                                          1650 J
                                                0.34±0.03
                                                0.1010.009
                                                assumed
                                                  0.10
        Figure 8.  Pollen profile  at  St. Leonard's
                   Creek in the  lower Patuxent River

-------
                                                          DIATOMS
                                                                                       MACROPIIYTES
00
PERIPIIYTIC- |

1
ID
•H ;H
•£ D*

10 q
•3 §

•Q 3

(0 i 1
•M 1 |
•H -H
*H *M
•3 3 t!
ID i fl KJ
PLANKTONIC
1
U
'3

1
q
q
. f! .. S
?
~T1



q.75
*H -Q
•Q m
3 ** • ffl
*o o €
ry o
>,

1980 "






1900-


1800-

.1600-
1400-

1200-
X





o
TJ •«•
§<•
U
•O
§_
iH
*
1
•H
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S.
1
a
0*
U W
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•H H
°> Q
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o 1
N \

r~t '
M
& ;




i
H ^
ti
S
                                                       i
                                             Q,Q,C4JVi
                     mco
                     -i-o-c!
                     Cl,<~lQ,
                       Q.4I
                       -
                                                       , ^
                                      1x10'
                                                               All scales 1x105
                                                    No. frustulos deposited cm   y
cou
3qw
CCON
5-14J
o  -1
"t
                                                   |seeds present

                                                  (data, .from 3 cores)
Figure  9.
                                                   Diatom and macrophyte  profile at
                                                   St.  Leonard's  Creek in the  lower
                                                   Patuxent  River.

-------
     The macrophyte seed record at St.  Leonard Creek is scant,  consisting of
Zannichellia palustris, Ruppia maritima,  Potamogeton perfoliatus v.  bupleu-
roidis (not shown)  and Zostera marina (riot shown).   Profiles from 3 cores
suggest that Ruppia maritima and Zannichellia palustris grew locally for at
least 3 centuries (Figure 9).   Aerial photographs and field surveys document
the decline of marcophytes in this section of the estuary since the late
1960's (Stevenson and Confer 1978; Anderson and Macomber 1979).

     Diatom and macrophyte populations preserved in recent sediments indi-
cate that the Upper Patuxent River, like Furnace Bay, was a shallow, probably
clear, mesotrophic embayment supporting a diverse macrophyte and predomi-
nantly periphytic diatom population prior to European settlement.  Gradually,
as agriculture was succeeded by urbanization, Jug Bay changed into a highly
eutrophic system dominated by species of Cyclotella, which can occur as
plankton.  At the same time, the macrophyte population was greatly reduced.
The record also-indicates that the deeper mesohaline part of the river was
affected only slightly by human activity, principally by an increase in
Cyclotella kutzingiana coincident with the deterioration of water quality in
the late 1960's and early 1970's  (Roberts and Pierce 1976).  Downstream,
where there has been little change in water quality, there is no detectable
change in diatom and macrophyte populations over several centuries.          :
WARE RIVER

Midstream

     The pollen profile  (Figure 10) shows the early settlement horizon  (^
1650) at 63 cm where ragweed increases from < 1 to > 1 but < 5 and the ratio
of oak to ragweed changes from > 10 to ^ 5.  The 1840 horizon is at 52 cm
based on an increase in percent ragweed to > 5 and a decrease in the oak-
ragweed ratio to < 3.  Based on these horizons, the sedimentation rate be-
tween mid-17th and mid-19th century was 0.0610.009 cm y"1.  Because it is
unlikely that the pre-settlement sedimentation rate would have been higher
at least in the decades immediately preceding settlement, we assumed a 0.06
cm y"1 rate for pre-settlement sediments.  The average rate since the mid-
19th century to the present is 0.36±0.03 cm y""1.

     Ninety-seven diatom species were identified in this core, of which  18    ;
occurred >. 3%  (Figure 11).  Total flux was never high here, but prior to
European settlement, the flora was extremely sparse; however, most species
which occurred later were also represented in these early sediments.  After
settlement, there was a slight increase in most species.  The eutrophic
periphyte, Opephora martyrii  (Lowe 1974) appeared in the late 1800's at  about
the time fertilizers were added to the watershed.  Cocconeis placentula  also
appeared about the same time.  An increase in flux since 1950 is represented  '
mainly by increases in Achnanthes temperii and Cocconeis fluviatilis.         :
                                     19

-------
NJ
O
Sediment TP Rag- Dated Sedimentation
Depth (no/g weed O:R Horizon Rate
(cm) sed) ;
n




40-





80-


120-
Dark gray
silt, clay
and sand


Gray-

Brown

Silt

and .
Clay

I
1
(
\
\
/
^^
^>
^^
>
\
\
/
{
\






}
1
1


.
t.
(cm y-1)
— i oor\ —















\
I
/
/
L
^ — . •* 	 1840 -
\
-i — 	 1650 -
?v.


\
\
*



0.36±0.03



0.06±0.009




assumed
0.06

                                     IxlO1*
5%
                              Figure  10.   Pollen profile at the midstream
                                           location in Ware River.

-------
to
(-1
                                                              PERIPHYTIC
•H ID


  10
  0)




1900-
                                              o
                                               f
                                              >.f
                                              C
       *
      
-------
Downstream

     The pollen profile  (Figure 12) at this location of the river shows the
1650 horizon at 88 cm based on a first appearance of ragweed.  The 1840 hori-
zon is located at 55 cm based on an increase in percent ragweed to between
5 and 15 and a decrease in the oak-ragweed ratio to < 2.  These dated hori-
zons yield sediment rates of. 0.1710.01 cm y"1 during early settlement and
0.39±0.03 cm y"1 during intensive agriculture.

     As in the core taken at midstream, total diatom flux is extremely low
prior to settlement (Figure 13).  Ninety-seven species were enumerated; 15
occurred in percentages k 3.  The flora consists of several species of peri-
phytes and 5 species of Cyclotella.  Most of the species were present prior
to European settlement.  Then the flora was dominated by Cyclotella bodanica
which is mesotrophic to eutrophic, Diploneis smithii and Cyclotella kutzingi-
ana.  The increase of flux with settlement was represented by an increase in
most species and by the first appearance of Achnanthes perpusilla.  At the
time fertilizers were added to the land, Cyclotella chaetoceios and Fragilar-
ia virescens appeared, and again flux increased with an increase in most
species.  Since 1950, flux has tripled due largely to increases in Cyclotella
chaetoceros, C. kutzingiana, C.k. planetophora, and Fragilaria virescens.
Water quality and available historical data do not provide an explanation for
this sudden large increase in species that are not necessarily periphytic but
may also be planktpnic.  Records from the other cores studied suggest that a
local source of nutrients probably stimulated the growth.

     The poor seed record in all cores collected in the Ware River did not
allow a reconstruction of macrophyte populations in the southern region of
the Chesapeake Bay.

     The stratigraphic record of diatoms indicates that the Ware River,
prior to European settlement, was  a highly oligotrophic 'estuary draining a
watershed underlain entirely by nutrient-poor Coastal Plain sediments.  After
land clearance, abundance increased particularly as chemical fertilizers were
added to the watershed.
                                      22

-------
00   >
                     Depth
                     (cm)
                        0
 Sediment
    TP   i
(no/g sed)
Rag-
weed
O:R
                       40-
                       80-
                      110-
                           Dark gray
                           silt and
                              clay
 Gray-
Brown
 Silt
  and
 Clay
 Gray
 Silt
 and
 Clay
                                     1x10"
              5%
                                 "1
                                  1
  Dated
 Horizon
— 1980 -i
Sedimentation
    Rate
   (cm y'1)
                                                                        1840  =^
                                                                       •1650  n
                                                                                0.3910.03
                                                                                0.17±0.01
                                                                                assumed
                                                                                  0.1
                              Figure 12.   Pollen profile at the downstream
                                          location in Ware River.

-------
                                                         PERIPIIYTlfc
                             PERIPHYTIC/PLANKTONIC
NJ
                                 1400-
                                 1200-
                                                                       I   T
:* a
•u 
-------
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                                      25

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                                     28

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