FINAL REPORT
TOXIC POINT SOURCE ASSESSMENT OF INDUSTRIAL
DISCHARGES'TO THE CHESAPEAKE BAY BASIN. PHASE in
PROTOCOL VERIFICATION STUDY
VOLUME III
APPENDICES D-E
Contract 68-02-3161
August 1982
MONSANTO RESEARCH CORPORATION
A SUBSIDIARY OF MONSANTO COMPANY
Monsanto
DAYTON
LABORATORY
DAYTON, OHIO 454OT
-------
903R81106
TOXIC POINT SOURCE ASSESSMENT OF. INDUSTRIAL DISCHARGES TO THE
CHESAPEAKE BAY BASIN. PHASE III: PROTOCOL VERIFICATION STUDY
VOLUME III
APPENDICES D-E
by
S. C. Wilson
B. M. Hughes
G. D. Rawlings
Monsanto Research Corporation
Dayton, Ohio 45418
Contract 68-02-3161
August 1982
Project Officer
Mark Alderson
Chesapeake Bay Program
U.S. Environmental Protection Agency
Annapolis, Maryland 21401
CHESAPEAKE BAY PROGRAM OFFICE
TOXICS PROGRAM
U.S. ENVIRONMENTAL PROTECTION AGENCY
ANNAPOLIS, MARYLAND 21401
-------
CONTENTS
Appendices
D. Phase III Plant-by-Plant Analysis Results
for Effluents ..."... D-l
E. Phase III Plant-by-plant Analysis Results
for Sediments E-l
111
-------
APPENDIX D
PHASE III PLANT-BY-PLANT ANALYSIS RESULTS FOR EFFLUENTS
Section
D.I
D.2
D.3
D.4
D.5
D.6
D.7
D. 8
D.9
D.10 •
D.ll
D.12
D.13
D.14
D.15
D.16
D.17
D.18
D.19
D.20
D.21
D.22
D.23
Plant
A109 : Organic and Inorganic Chemicals Manufacture
B112D: Wood Treating and Preserving
B119D: Plastics Manufacture
C150D: Municipal Sewage Treatment Plant ....
C157D: Nylon Resins and Fibers Manufacture . . .
C161D: Municipal Sewage Treatment Plant ....
C164D: Combined Municipal/Industrial Sewage
C169D: Combined Municipal/Industrial Sewage
B133S: Chemical Manufacture ....
B141S: Combined Municipal/Industrial Sewage
B142S: Industrial Waste Treatment and Recovery .
B149S: Stainless Steel Manufacture .
A101: Synthetic Organic Fibers Manufacture . . .
CIS ID: Commercial Electric Power Generation . .
C154D: Ammoniated Fertilizer Production ....
C158D: Combined Municipal/Industrial Sewage
C159D: Pork Slaughter, Processing, and Packaging
Page
D.l-1
. D.2-1
. D.3-1
. D.4-1
. D.5-1
. D.6-1
. D.7-1
. D.8-1
. D.9-1
D.10-1
D.ll-1
D.12-1
D.13-1
D.14-1
D.15-1
D.16-1
D.17-1
D.18-1
D.19-1
D.20-1
D.21-1
D.22-1
D.23-1
D-l
-------
Section Plant Page
D.24 C160D: Rubber Product Manufacture D.24-1
D.25 B126S: Inorganic Pigment Manufacture D.25-1
D.26 B143S: Titanium Dioxide Pigment Production . . . D.26-1
D.27 B147S: Stainless Steel Manufacture D.27-1
D.28 C169S: Industrial Inorganic Chemicals Production D.28-1
D.29 References D.29-1
D-2
-------
APPENDIX D
PHASE III PLANT-BY-PLANT ANALYSIS RESULTS FOR EFFLUENTS
D.I PLANT A109: ORGANIC AND INORGANIC CHEMICALS MANUFACTURE
D.I.I Process Description
Plant A109 is a chemical manufacturer producing several types of
inorganic and organic chemicals. Sodium hydrosulfite, sodium
metabisulfite, zinc sulfate, and specialty amines are the major
products from this site (SIC 2819, 2811, and 2869).
Figure D.l-1 is a water flow and wastewater treatment diagram for
the plant. Wastewater is generated by all the major processes.
The wastewater from the hydrosulfite, metabisulfite, and sulfur
dioxide processes is collected in treatment sumps for pH adjust-
ment by lime and sulfuric acid prior to being sent to an aeration
tank. Amine wastewater is treated separately in an aeration
lagoon (retention time approximately 40 days) before it is com-
bined and treated with the other process wastewaters. After the
aeration tank, where all wastewaters are combined, a two-stage'
settling pond and a polishing pond remove settleable material
before final discharge. The total plant effluent flow rate is
1.1 MGD.
D.I.2 Analysis Results, Plant A109
The following tables in this section present the data collected
for Plant A109.
D.l-1
-------
WATER
SOURCES"
I
to
ZINCSULFATE
SODIUM
META-SULFITE
SODIUM
META-BISULFITE
SULFUR
DIOXIDE
AMINE
ZINC CARBONATE
SETTLING POND
COLLECTION
AND
TREATMENT SUMP
COLLECTION
AND
TREATMENT SUMP
AERATION
LAGOON
2 - STAGE
SETTLING POND
SAMPLING
POINT
DISCHARGE
Figure D.l-1. Water flow and waste treatment diagram for Plant A109:
inorganic and specialty organic chemicals production.
-------
TABLE D.l-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT A109
Temper- Dissolved
Flow, ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments '
17.2 Salinity =23.5 ppt; plant operating
NAa 8.3 (63.0) 2.5 normally; effluent green in color
Data not available; no reading taken.
TABLE D.l-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT A109
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (ng/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Total phosphorus
Total dissolved phosphorus
_a
46
<0.020
<0.010
221
<0.20
<0.20
26.0
23.8
<0.04 .
<0.25
6,000
3,200
<2.5
0.627
I*
6
1.0
0.020
0.010
0.1
0.20
0.20
mr
NR
0.04
0.25
0.5
1.0
2.5
NR
NR
aSample not analyzed.
No detection limit reported
cSample improperly preserved.
D.l-3
-------
TABLE D.l-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
A109, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal Standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected0
15.1 Chloroform <10d
Total concentration <10
Background corrected based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
GQuantitated relative to area of major ion of
authentic standards.
10 H9/L is quantitation limit of computerized
identification system.
eSummation of concentrations of detected
compounds, excluding standards.
D.l-4
-------
01
TABLE D.l-4 ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
A109 CORRECTED FOR BACKGROUND '
Peak
No.
6
3
1
2
Retention
time,
min
21.10
6.53
3.38
4.08
Compound
Internal standard0
Anthracene-d10
Recovery standard '
Phenol-d5
Organics detected '"'
Unknown (83,60,39,97)
2 - Cyc lohexen- 1 - ol
Peak area,
total counts
281,391
51,995
5,081
3,053
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
100
53.1 223 23.8
2.3
1.4
(continued)
-------
TABLE D.l-4 (continued)
0
' *
H
I
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked. Recovery,
total counts pg/L ug/L percent
Organics detected
(continued)
f,9,h
4
5
7.17 Acetamide, N,N-diethyl- 18,021
7.45 Cl-containing unknown
(57,80,81,88,98,134) 7,986
Total concentration
8.2
3.7
15.6
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH
peak identification program in the Hewlett-Packard system, and are greater than
1.4 ng/L relative to the anthracene-d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 (Jg/L in original sample.
oeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated related to area of major ion authentic standard.
Estimated concentration based on area of total ion relative to that of
anthracene-djo, internal standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parenthesis according
to decreasing intensities. Possible molecular ions are underlined.
Summation of concentrations detected compounds, excluding standards.
-------
TABLE D.l-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT A109 CORRECTED FOR BACKGROUND- '
i
-j
RRI
202 . 34
_j
-
298.36
397.91
499.26
_
-
-
-
-
106.32
Peak
No.
13
1
8
14
15
16
2
3,4
5
6
7
9
Retention
time ,
mm
21.10
7.65
14.77
25.20
29.02
33.1
8.98
9.32
11.32
11.68
13.63
15.22
Compound
Internal standard
Anthracene-d10
Recovery standard '
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d!2
Perylene-d12
Organics detected 'g'
Unknown (57,97,112,141,
148)
Unknown (86,72,101,157,
172)
Unknown (97,82,81,124)
Unknown (183,113,71,127)
Unknown (183,127,85,198)
Unknown (95,137,151,179)
Peak area.
total counts
167,456
119,621
170,361
123,643
45,922
28,209
77,401
4,230,025
34,737
975,171
59,043
38,819
Amount
detected,
Mg/L
100
50.8
60.8
66.7
45.7
44.5
46.2
2,526
20.7
582
35.3
23.2
Amount
spiked. Recovery,
pg/L percent
-
118.1 43.0
101.6 59.8
99.3 67.2
85.8 53.3
90.6 49.1
1
(continued)
-------
TABLE D.l-5 (continued)
oo
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
ug/L
Amount
spiked,
Recovery,
percent
Organics detectedf/g/h
(continued)
127.07
153.81
198.06
10
11
12
16.50
18.15
20.88
Unknown (86,99,72,185)
Unknown (187,141,99,188)
Unknown (127,57,71,86)
Total concentration1
418,763
52,242
85,856
250
31.2
51.3
3,566
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found a or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 20.7 pg/L
relative to the anthracene-d10 internal standard.
£
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
oeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10
internal standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensities. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE D.l-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT A109
Concentration
Purgeable
Analysis organics
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS NDa ' ^
Carbon balance
% Purgeable
% Extractable6 f
% Chromatographable
% GRAV Chromatographable^
% Extractables identified
Protocol identification efficiency , %
in each fraction, mg/L
Extractable organics
Base/
Acid neutral Total
46
0.35 1.5 1.85
0.08 0.75 0.83
0.10. 6.00 6.10
0.02° 3.6C <3.63
17.3
15.1
• 44 . 9
7.9
<8.0
aFrom Table D.l-3. .
From Table D.l-4.
cFrom Table D.l-5.
^ of carbon tmracablc - Total mass of Pur9eables x inn
ey of carbon oxtrartnhl o - ^®-.+ GRAV 1 00
^°/ nf r-arhnn r-hrnma1-nnrar»hahl (* - ™ ^ + ™^^* v 1 nn
X L^vJ
of grav Chromatographable = x 100
of extractables identified = Total mass of acidGC/MS + B/N GC/MS x 10Q
Protocol organics identification efficiency summation of Equations d and h.
indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.l-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT A109, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
22
<400
2,140
39
<5
524,000
<10
47
571
<30
1,960
96.7
121,000
423,000
74.5
<50
' 2,400,000
180
<350
<200
2,740
<75
45.1
<25
<200
not reported.
D.l-10
-------
TABLE D.I-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT A109 EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16 '
17
t , min
2.17
3.13
3.72
3.93
5.43
5.88
6.80
8.73
9.46
11.89
13.25
16.63
18.30
19.20
19.95
26.50
44.58
Log tr
0.3365
0.4955
0.5705
0.5944
0.7348
0.7694
0.8325
0.9410
0.9759
1.0752
1.1222
1.2209
1.2625
1.2833
1.2999
1.4232
1.6491
Calcd.
log P
0.59
1.35
1.70
1.82
2.48
2.65
2.95
3.47
3.63
4.10
4.33
4.80
4.99
5.09
5.17
5.76
6.83
Peak
height,
cm
4.5
0.4
>15
12.6
1.2
1.0
5.5
2.4
5.5
1.1
0.5
0.3
0.2
0.1
0.1
0.15
9.5
Adjusted
peak
height,
cm
410
36
>1,400
1,100
110
91
500
220
500
100
46
27
18
9
9
14
870
Estimated
. concentration Positive
FSD , index , or
% ppb negative
l,700d
240
>9,100
7,700
730
610
3,300
1,500
3,300
670
300
180
120
61
61
91
5,800
110
10
>360
2646
29
24
<16
536
<1 +
27 +
12 +
7 +
5 +
2 +
4 +
4 +
230 +
Retention time.
Percent full-scale deflection
"A positive response is defined as lop P^3.5 with % FSD >25.
See Appendix C for an explanation of FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.l-9. CORRELATIONa OF BIOAGCUMULATION POTENTIAL13 AND
GC/MS ANALYSIS TEST RESULTS: PLANT A109
Organic compounds identified by Molecular
GC/MS analysis formula
Literature
log P value [1]
Actual sample
log P values
by HPLC method
(Pentachlorocyclohexene) CgHsCls 3.61
(Hexachlorocyclohexene) C6H4C16 4.12
.
2-Cyclohexen-l-ol CgHjoO
Acetamide, N,N-diethyl- C6H13NO
3.63
4.10
4.33
4.80
4.99
5.09
5.17
5.76
6.83
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate
(log P £3.5 and £25% FSD).
CTentative identification based solely on log P values and similar
compounds in sample.
NO literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
D.l-12
-------
TABLE D.l-10. CALCULATED LC50'S FOR MYSID SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE A109 IN
STATIC, UNAERATED ARTIFICIAL SEAWATER
Hour
24
48
72
96
LCso ,
% effluent
>100
74
32
24
95% confidence limits,
% effluent
_b
63-86
24 - 43
18-33
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based on
nominal concentrations of test
material in artificial seawater.
pH remained within acceptable lim-
its throughout the test. Low dis-
solved oxygen in 100% effluent
would appear to be the major cause
of mortality in this test concen-
tration.
aAssay performed by E.G. & G. Bionomics;
Pensacola, Fla.
Confidence limits not calculated.
TABLE D.l-11. FATHEAD MINNOW BIOASSAY
RESULTS FOR PLANT A109
LC50 value,
Time % of neat effluent
24-hour -b
48-hour
72-hour
96-hour >100
aAssay performed by State
Water Control Board,
Commonwealth of Virginia.
Data not reported.
D.l-13
-------
D.I.3 Data Interpretation
D.I.3.1 Evaluation of Protocol Performance—
The wastewater effluent from plant A109 showed moderate toxicity
to mysid shrimp (LC50 = 24%; 96 hr), but no toxicity to fathead
minnows. From the inorganic and ionic analysis, potential sources
of the toxicity"include ammonia-nitrogen at 23.8 mg/L (basic waste-
water at pH 8.3) and a chromium concentration of 571 M9/L. Even
though the TOC value of 46 mg/L was below the protocol decision of
50 mg/L, further analysis of the organic content was initiated by
GC/MS. While the purgeable organics and the acidic organics from
the extraction were at very low concentration, the base-neutral
organics were very high (total concentration = 3.566 mg/L). Un-
fortunately no compounds in the base-neutral fraction were identi-
fied but are assumed to be amines or amine derivatives.
D.I.3.2 Protocol Changes Suggested for Plant A109—
In this case the LC fractionation to assist in GC/MS identification
was not employed even though it is a step in the protocol. If the
LC fractionation had been conducted, characterization of the base-
neutral extractables would have been more complete.
D.I.3.3 Potential Sources of Chemical Toxicity--
Potential sources of the moderate toxicity noted in Plant A109
wastewater include ammonia (23.8 mg/L), chromium at 571 ug/L and
possible amines and/or amine derivatives from the base-neutral
extractabls that were not characterized.
D.l-14
-------
D.2 PLANT B112D: WOOD TREATING AND PRESERVING
D.2.1 Process Description
3
Plant B112D is engaged in wood treating and preserving via batch
processes using pentachlorophenol and creosote (SIC 2491).
Products include treated pilings, poles, ties, and sawed lumber.
Figure D.2-1 is a wastewater treatment diagram for the plant.
Plant wastewater is burned in a liquid injection incinerator, and
most of the storm runoff is collected in a 24-inch surface runoff
pipe which contains two 10 ft by 3 ft polymeric sorbent beds just
prior to final discharge.
PLANT
WASTEWATER
LIQUID
INJECTION
INCINERATOR
\
ASH TO
LANDFILL
STORM
RUNOFF "
POLYMERIC
SORBENT
BEDS
DISCHARGE
i
J
i
* SAMPLING
9 POINT
Figure D.2-1
Wastewater treatment diagram for Plant B112D:
wood preservation and testing.
D.2-1
-------
D.2.2 Analysis Results, Plant B112D
The following tables in this section present the data collected
for Plant B112D.
TABLE D.2-1.
SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B112D
Flow,
m3/d (MGD)
PH
Temper- Dissolved
ature, oxygen,
°C (°F) mq/L
Comments
NA 6.7 15.0 6.1 Effluent at outfall 001 insufficient;
(59.0) sampled at manhole below 001 (combined
discharge from several plants, 75%
from Plant B112D). Effluent smelled
of creosote and had visible oil sheen;
rain (0.5 in.) previous night.
Data not available, no reading taken.
TABLE D.2-2.
WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B112D
(mg/L, except as noted)
Species
Detection
Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended soilds
Total mercury, ng/L
Filtered mercury, pg/L
Total Kjeldahl nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
18
<0.020
0 . 344
15.3
<0.20
<0.20
1.98
0.3
43
36
5.1
0.088
0.246
0.129
1.0
0.020
0.010
0.1
0.2
0.2
NR3
0.25
0.5
1.0
2.5
0.007
NR
NR
aNo detection limit reported.
D.2-2
-------
TABLE D.2-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B112D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane . 400
Organics detected^
6.8 Dichlorodifluoromethane <^-^d
15.1 Chloroform <10d
17.1 1,1,1-Trichloroethane
-------
TABLE D.2-4 ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
B112D CORRECTED FOR BACKGROUND3'D
JO
I
Peak
No.
48
1
2
3
4
5
6
7
8
9
10
Retention
time,
min
21.10
6.52
7.95
8.32
9.48
10.22
10.47
10.93
11.07
11.25
11.37
Compound
£
Internal standard
Anthracene-d10
Recovery standard '
Phenol-d5
Orqanics detectedf 'g/h
Benzaldehyde , hydroxy-
(isomer) plus 50%
unknown
Phenol , 3-methyl-
Phenol, dimethyl-
(isomer)
Phenol, ethyl- (isomer)
Phenol , dimethyl-
( isomer)
Phenol, dimethyl-
( isomer)
Phenol , dimethyl-
(isomer)
Benzo[b] thiophene
Phenol, dimethyl-
• (isomer)
Peak area,
total counts
208,639 -
60,213
7,331
6,228
58,452
9,604
301,994
330,368
51,617
18,751
51,177
Amount Amount
detected, spiked, Recovery,
|jg/L pg/L percent
100
73.6 223 33.0
3.5
3.0
28.0
4.6
145
158
24.7
9.0
24.5
(continued)
-------
TABLE D.2-4 (continued)
0
to
i
in
Peak
No.
11
12
13
14
15
16
17
18
21
22
23
Retention
time,
min
11.55
11.73
12.10
12.27
12.35
12.70
12.80
12.90
13.75
14.15
14.27
Compound
Organics detected '"'
(continued)
Phenol, trimethyl-
(isomer)
Benzofuran, 7-methyl-
plus 10% unknown
70% IH-Inden-l-ol,
2,3-dihydro- plus 30%
phenol, trimethyl-
(isomer)
Phenol, ethylmethyl-
(isomer)
Phenol, ethylmethyl-
(isomer)
Phenol, 2-ethyl-5-
methyl-
Phenol, trimethyl-
(isomer)
Phenol, trimethyl-
(isomer)
Benzaldehyde , dimethyl-
(isomer) plus 20%
unknown
IH-Inden-l-ol, dihydro-
(isomer)
Unknown (150, 121, 135,
122)
Peak area,
total counts
13,947
20,407
48,354
45,562
28,488
108,109
24,861
39,495
65,365
51,060
16,816
Amount Amount
detected, spiked, Recovery,
|jg/L Mg/L percent
6.7
9.8
23.2
21.8
13.7
51.8
11.9
18.9
31.3
24.5
8.1
(continued)
-------
TABLE D.2-4 (continued)
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pg/L |jg/L percent
Organics detected
(continued)
f,g,h
to
1
24
25
27
28
29
30
31
32
14.35
14.60
15.45
15.65
15.73
15.98
16.47
16.57
Ethanone, l-(4-ethyl-
phenyl)- or ethanone,
l-(2,4-dimethyl-
phenyl)- plus 25%
unknown (MW =150)
plus 25% C13 contain-
ing unknown (MW = 196)
Ethanone, l-(4-ethyl-
phenyl)- or ethanone.
l-(2,4-dimethyl-
phenyl)-
Benzene, pentamethyl-
(isomer)
Benzene, pentamethyl-
(isomer)
Unknown (147, 133, 148,
135)
Benzene, dimethyl- (-1-
methylethyl)- (isomer)
IH-Indole, 2,3-dihydro-
4-methyl- plus 10%
unknown
Acenaphthylene ,
1,2-dihydro-
11,280
12,880
9,882
7,510
5,691
21,438
5,460
10,631
5.4
6.2
4.7
3.6
2.7
10.3
2.6
5.1
(continued)
-------
TABLE D.2-4 (continued)
N)
i
-j
. ....... .. .. .
Peak
No.
33
34
35
36
37
38
39
40
41
42
Retention
time,
min
16.75
17.08
17.33
17.55
17.93
18.12
18.60
19.03
19.67
19.92
Peak area,
Compound total counts
Organics detected '"'
(continued)
(C2)3-benzene isomer
plus 20% unknown
compound
Unknown (170, 169, 168,
141) spectrum similar
to [l,l-biphenyl]-2-ol
Unknown (150, 121, 122)
Phenol, 2,3,4,6-tetra-
chloro-
IH-Indole, 2,3-dihydro-
plus 40% unknown
(MW = 147); possibly
pyridopyrimidin-one
(isomer)
Unknown (MW =161)
1-Naphthalenol, 3-
methyl- plus 25%
unknown
(MW = 164)
1-Naphthalenol ,
3-methyl-
Naphthalenecarboxylic
acid (isomer)
Naphthalenecarboxylic
acid (isomer)
6,480
8,110
7,874
9,531
18,242
4,934
4,061
23,803
16,370
20,463
Amount Amount
detected, spiked, Recovery,
ug/L (jg/L percent
3.1
3.9
3.8
4.6
8.7
2.4
2.0
11.4
7.8
9.8
(continued)
-------
TABLE D.2-4 (continued)
Retention
Peak time,
No . . min
Compound
Amount Amount
Peak area, detected, spiked,
total counts pg/L M9/L
Recovery,
percent
Organics detected 'g'
(continued)
43
44
50
51
52
53
20.07
20.17
o
to
i
00
45
46
47
49
20.68
20.77
20.98
21.50
21.88
22.07
22.18
22.92
50% l(2H)-Isoquinolinone
and 50% 1 , 1 ' -biphenyl ,
3-ethoxy- 39,340
50% Isoquinoline, 2-
oxide and 50% 1(2H)-
isoquinolinone 1,175
Phenol, pentachloro- 17,248
2-Dibenzofuranol 9,974
Phenanthrene 2 , 206
Phenol, (phenylmethyl)-
( isomer) plus 20%
unknown 6,165
Unknown (MW = 184);
possibly dibenzo-
thiophene or dibenzo-
furanol (isomer) 6,240
Dibenzofuranol (isomer)
plus 10% unknown 10,432
Dibenzo[b,e][l,4]dioxin
or dibenzofuranol
(isomer) plus unknown 11,456
[1 -Biphenyl] -carbox-
aldehyde (isomer)
plus 10% unknown 7,390
18.9
0.6
8.3
4.8
1.1
3.0
3.0
5.0
5.5
3.5
(continued)
-------
TABLE D.2-4 (continued)
to
i
\D
Peak
No.
Retention
time,
min
Compound
Peak
total
area,
counts
Amount
detected,
M.g/L
Amount
spiked,
pg/L
i
Recovery,
percent
Organics detected g'
(continued)
54,
55
56
57
58
23.68
24.33
25.20
29.92
2,4(lH,3H)-Pyrimidine-
dione, 5-bromo-6-
methyl-3-(l-methyl-
propyl)-
lH,3H-Naphtho[l,8-cd]-
pyran-1 ,3-dione
Fluoranthene or pyrene
Unknown phthalate
Total concentration1
268,112
15,183
5,203
5,362
129
7.3
2.5
2.6
915
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 0.6 Mg/L
relative to the anthracene d10 internal standard.
^
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 ug/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of
anthracene-d10, internal standard.
"Tentative identification, not confirmed with authentic standards.
masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.2-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT B112D CORRECTED FOR BACKGROUND '
O
to
RRI
202.81
298.83
398.17
498.51
Peak
No.
41
1
17
47
51
52
Retention
time,
min
21.12
7.65
14.77
25.22
29.03
33.07
Compound
Internal standard0
Anthracene-d10
Recovery standard '
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '"'
Peak area,
total counts
163,855
142 , 846
241,776
142,423
64,272
58,828
Amount
detected,
pg/L
100
70.7
78.1
76.9
63.1
77.6
Amount
spiked,
pg/L
-
118.1
101.6
99.3
85.8
90.6
Recovery,
percent
-
59.9
76.9
77.4
73.5
85.7
4
5
7.75 IH-Indene, 2,3-dihydro-, and/or
benzene, propenyl-, (isomer)
and/or benzene, ethenyl
methyl- (isomer) 149,317
8.58 Benzene, isocyano methyl- (isomer),
and/or benzeneacetonitrile, and/
or benzonitrile, methyl-, and/or
benzaldehyde, 4-methyl-, oxime,
(E)- (isomer) 26,422
9.48 Phenol, dimethyl- (isomer) 37,690
10.43 Phenol, dimethyl- (isomer) 62,109
91.1
16.1
23.0
37.9
(continued)
-------
TABLE D.2-5 (continued)
N>
I
RRI
_
-
-
-
-
-
-
-
-
-
-
100.00
106.81
110.86
111.67
116.20
129.01
131.60
136.79
154.13
Peak
No.
6
7
8
9
10
11
12
13
14
15
16
18
20
21
22
23
24
25
26
31
Retention
time,
min
10.88
11.25
11.55
11.75
12.12
12.27
12.70
12.82
12.90
13.05
13.57
14.83
15.25
15.50
15.55
15.83
16.62
16.78
17.10
18.17
Compound
Organics detected /g' (cont'd)
Phenol, dimethyl- (isomer)
Benzo [b] cyclobuta [ d] thiophene-1 ,
2-dicarboxylic acid, l,2,2a,
7b-tetrahydro-l , 2-dimethyl- ,
and/ or benzo[b]thiophene
Phenol, trimethyl- (isomer)
Benzaldehyde , ethenyl-, and/or
benzofuran, 7-methyl-
IH-Inden-l-ol, 2,3-dihydro- plus
10% phenol, trimethyl- (isomer)
Phenol, ethylmethyl- (isomer)
Phenol, ethylmethyl- (isomer)
Phenol, trimethyl- (isomer)
Phenol, trimethyl- (isomer)
IH-Inden-l-one, 2,3-dihydro-
Unknown (142, 143, 141, 115)
1,1 '-Biphenyl
Naphthalene, dimethyl- (isomer)
Naphthalene, dimethyl- (isomer)
Naphthalene, dimethyl- (isomer)
Naphthalene, dimethyl- (isomer)
Acenaphthylene , 1 , 2-dihydro-
1-Naphthalenecarbonitrile
Dibenzofuran
9H-Fluorene
Peak area,
total counts
19,419
33,814
58,784
69,037
99,251
32,820
25,799
20,439
23,922
65,277
224,828
40,726
64,642
85,444
51,514
17,035
496,961
55,941
207,532
318,843
Amount Amount
detected, spiked, Recovery,
|jg/L M9/L percent
11.8
20.6
35.9
42.1
60.6
20.0
15.7
12.5
14.6
39.8
137
24.8
39.4
52.1
31.4
10.4
303
34.1
127
195
(continued)
-------
TABLE D.2-5 (continued)
RR1
157
166
174
176
0 181
•
.54
.29
.07
.82
.69
*? 193.84
£ 200
207
218
236
242
285
.70
.03
.74
.30
.86
.01
Peak
No.
32
35
36
37
38
39
40
42
43
44
45
46
Retention
time. Peak area,
min Compound total counts
18.38
18
19
19
19
20
21
21
21
22
22
.92
.40
.57
.87
.62
.03
.30
.80
.55
.83
24.63
Organics detectedf 'g'h (cont'd)
Unknown (71, 85, 57, 155, 226)
Dibenzofuran, 4-methyl-
[l,l'-Biphenyl]-2-ol plus
20% impurity
50% Benzene, 1,1 '-(1 ,2-ethene-
diyl)-bis- plus 50% unknown (161,
132, 133)
70% 9H-fluorene, 1-methyl plus
30% unknown (161, 132, 133)
Dibenzothiophene plus 10% unknown
Phenanthrene
Benzo[h]quinoline, and/or pyridine,
2-(phenylethynyl)- and/or phenan-
thridine, and/or acridine, and/or
[l,l'-biphenyl]- 2-carbonitrile
l,l'-Biphenyl, 2-azido-, and/or 9H-
carbazole, and/or 1-naphthyl
isocyanide, 2-methyl-
Phenanthrene , 4-methyl- plus 10%
unknown
4H- Cyc lopenta [ de f ] phenanthr ene
Fluoanthene
19,
44,
79,
48,
34,
38,
192,
16,
60,
19,
40,
94,
342
294
425
124
312
568
902
506
584
989
665
525
Amount Amount
detected, spiked, Recovery,
Mg/L pg/L percent
11.
27.
48.
29.
20.
23.
118
10.
37.
12.
24.
57.
8
0
5
4
9
5
2
0
2
8
7
(continued)
-------
TABLE D.2-5 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area.
total counts
Amount Amount
detected, spiked, Recovery,
ug/L pg/L percent
Organics detected >g' (cont'd)
329.50 50 26.40 Benzo[c]cinnolin-2-amine, and/or
HH-benzo[b]fluorene, and/or
pyrene, methyl- (isomer), and/or
• acridinone, and/or 6(5H)-
phenanthridinone
Total concentration
31,060
19.0
1,836
0
ro
H
CO
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 10.2 |jg/L
relative to the anthracene dlo internal standard.
£
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 ug/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE D.2-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B112D
O
*
to
Concentration in each fraction
(mg/L)
Extractable organics
Analysis
TOC
GRAY
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeable
% Extractable6 f
% Chromatographable
% GRAV Chromatographable9.
% Extractables identified .
Protocol identification efficiency , %
Purgeable Base/
organics Acid neutral
5.78 4.88
0.96 1.72
0.96, 2.50
<0.04a 0.92° 1.82C
Total
18
10.66
2.68
3.46
2.78
<0.2
78.4
34.1
25.1
15.2
<15.4
From Table D.2-3*
From Table D.2-4.
'From Table D.2-5.
of carbon purgeable = Total massif purgeables x 100
e% of carbon extractable = TCO * GRAV x 100
X WV-.
f% of carbon Chromatographable = TC°JLTCG x 100
d.\J\*
o Tff*
y% of grav Chromatographable = x 100
h% of extractables identified
1Protocol organics identification efficiency summartion of Equations d and h.
of extractables identified = Total mass of acid^GC/MS + B/N GC/MS x
-------
TABLE D.2-7. ICAP METALS ANALYZED BY EPA REGION V,
PLANT B112D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3.0
240
202
48.6
<1.0
53,100
<2.0
<6.0
258
32.6
4,840
<10.0
8,200
8,900
219
<10.0
47,000
194
<70.0
157
533
<25.0
<5.0
<5.0
234
not reported.
D.2-15
-------
TABLE D.2-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B112D EFFLUENT
K)
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
t , min Log t
r r
1.42
2.03
2.32
2.75
3.20
3.59
3.93
4.41
5.06
5.45
5.80
6.85
9.09
9.49
10.47
10.90
12.50
13.21
14.35
17.76
19.66
21.56
23.10
24.40
0.1523
0.3075
0.3655
0.4393
0.5052
0.5551
0 . 5944
0.6444
0.7042
0.7364
0.7634
0.8357
0.9586
0.9773
1.0199
1.0374
1.0969
1.1209
1.1569
1.2494
1.2936
1.3336
1.3636
1.3874
Peak
Calcd. height,
log P cm
-0.29 0.3
0.45 >15
0.73 >15
1.08 >15
1.39 13.0
1.63 >15
1.82 >15
2.05 >15
2.34 >15
2.49 >15
2.62 >15
2.96 >15
3.55 13.9
3.64 >15
3.84 11.2
3.92 14.6
4.21 >15
4.32 >15
4.49 >15
4.93 >15
5.14 >15
5.33 6.1
5.48 3.2
5.59 7.4
Adjusted
peak
height,
cm
27
>1,400 '
>1,400
>1,400
1,200
>1,400
>1,400
>1,400
>1,400
>1,400
>1,400
>1,400
1,300
>1,400
1,000
1,300
>1,400
>1,400
>1,400
>1,400
>1,400
560
290
670
Estimated
. concentration
FSD , index ,
% ppb
180d
>9,100
>9,100
>9,100
7,900
>9,100
>9,100
>9,100
>9,100
>9,100
>9,100
>9,100
8,400
>9,100
6,800
8,900
>9,100
>9,100
>9,100
>9,100
9,100
3,700
1,900
4,500
<16
>360
>360
>360
320
>358e
>3146
>3536
>3586
>360
>360
>200
340
>2106
270
350
>360
>360
>360
>360
>360
150
78
180
Positive
or c
negative
.
-
-
-
_
_
_
_
-
-
_
-
.+
+
+
+
+
+
+
+
+
+
+
+
(continued)
-------
TABLE D.2-8 (continued)
to
i
Peak
number
25
26
27
28
29
30
31
32
t , min
27.49
30.50
32.00
33.88
36.88
38.15
41.45
47.88
Log tr
1.4392
1.4843
1.5052
1.5299
1.5668
1.5815
1.6175
1.6802
Calcd.
log P
5.83
6.05
6.15
6.27
6.44
6.51
6.68
6.98
Peak
height,
cm
9.6
2.7
1.7
1.5
2.0
2.7
2.8
1.1
Adjusted
peak
height,
cm
870
250
150
140
180
250
260
100
h
FSD,
*
5,800
1,600
1,000
910
1,200
1,600
1,700
670
Estimated
concentration Positive
index , or
ppb negative
230 +
66 +
41 +
36 +
49 +
66 +
68 +
27 +
Retention time.
b
Percent full-scale deflection.
CA positive response is defined as log P ^3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.2-9.
CORRELATION3 OF BIOACCUMULATION POTENTIAL13 AND
GC/MS ANALYSIS TEST RESULTS: PLANT B112D
Organic compounds identified by
GC/MS analysis
9H-Carbazole
1,1' -Biphenyl
2 , 3 , 4 , 6-Tetrachlorophenol
Dibenzofuran
9H-Fluorene
Dime thy Inaphthalene
Phenanthrene
Pyrenie
Pentachlorophenol
f
Molecular
formula
C12H9N
Ci2H10
C6H2C140
Ci2H80
CiaHio
C12H12
C14H10
C16H10
C6HC150
Literature
log P value [1]
3.76C
3.95d'g
4.10
4.12
4.18g
4.3ie'g
4.46y
4'88f a
5.12f'9
Actual sample
log P values
by HPLC method
3.55
3.64
3.84
3.92g
4.21g
4.32g
4.49g
4.93g
5.14g
5.33
5.48
5.59
5.83
6.05
6.15
6.27
6.44
6.51
6.68
1,1,2, -Trichloroethane
Benzofuran, 7-methyl-
IH-Inden-l-ol, 2,3-dihydro-
Ethyl methyl phenol
2-Ethyl-5-methyl-phenol
Dimethyl benzaldehyde
Ethanone, l-(4-ethylphenyl)-
Ethanone, l-(2,4-dimethyl-
phenyl
Pentamethyl benzene
Dimethyl-(1-methylethyl)-
benzene
IH-Indole, 2,3-dihydro-
4-methyl-
Acenaphthylene, 1,2-
dihydro
Triethyl benzene isbmer
IH-lndole, 2,3,-dihydro-
1-Naphthalenol, 3-methyl-
Naphthalenecarboxylic acid
C9H80
C9H100
C9H120
C9Hi20
C10H12°
C10H12°
C11H16
C11H16
C12H18
C8H9N
CnH100
(continued)
D.2-18
-------
TABLE D.2-9 (continued)
Organic compounds identified by Molecular
GC/MS analysis formula
Literature
log P value [1]
Actual sample
log P values
by HPLC method
l,l'-Biphenyl, 3-ethoxy- C14H140
Isoquinoline, 2-oxide- C9H7NO
2-Dibenzofuranol ci2H8°2
Phenol, (phenylmethyl) ci3Hi2°
Dibenzofuranol (isomer) ci2H8°2
Dibenzo[b,e][1,4]dioxin Ci2H8°2
[l-Biphenyl]-carboxaldehyde C13H100
2,4(1H,3H)-Pyrimidinedione, C9Hj 3N2•
5-bromo-6-methyl-3- 02Br
(1-methylpropyl)-
1H,3H-Naphtho[1,8-cd]pyran-
1,3-dione C12H603
Fluoranthene C16H10
IH-Indene, 2,3-dihydro- C9H10
Benzene, propenyl- C9H10
Benzene, ethynl-methyl- C9H10
Benzene, isocyano-methyl- C8H7H
Benzonitrile, methyl- C8H7N
Benzaldehyde, 4-methyl-,
(Oxime), (E)- C8H9NO
Benzo[b]cyclobuta[d]thiophene-
1,2-dicarboxylic acid, 1,2,
2a, 7b-tetrahydro-l,2-
dimethyl) ci4Hi4°4s
Phenol, trimethyl- C9H120
Benzaldehyde, ethynl- C9H80
Benzofuran, 7-methyl- . C9H80
IH-Inden-l-one, 2,3-dihydro- C9H80
1-Naphthalenecarbonitrile
Dibenzofuran, 4-methyl-
[l,l'-biphenyl]-2-ol C12H100
Benzene, 1,1'-(1,2-ethene-
diyl)-bis- C14H12
9H-Fluorene, 1-methyl- ci4Hi2
Dibenzothiophene C12H8S
Benzo[h]quinoline C13H9N
Pyridine, 2-(phenylethynyl)- C13H9N
Phenanthridine C13H9N
[1,1'-Biphenyl]-2-carbonitrile C13H9N
1,1'-Biphenyl, 2-azido- C12H9N3
1-Naphthyl isocycanide,
2-methyl- C12H9N
Phenanthrene, 4-methyl- ci5Hi2
4H-Cyclopenta[def]
phenanthrene ci5Hio
(continued)
D.2-19
-------
TABLE D.2-9 (continued)
Organic compounds identified by Molecular Literature
GC/MS analysis formula log P value [1]
Actual sample
log P values
by HPLC method
Benzo [c] cinnolin-2-amine
HH-Benzo[b] f luorene
Acridinone
6 ( 5H ) -Phenanthr idinone
C12H9N3
C13H9NO
C13H9NO
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
£3.5 and £25% FSD).
C0ther values reported are 3.29, 3.72.
Other values reported are 3.16, 4.04, 4.09, 4.17.
eOther values reported are 4.26, 4.37, 4.38, 4.40, 4.42, 4.44.
Other values reported are 3.81, 5.01, 5.86.
'Potential correlation.
rlo literature log P found.
[1] Hausch, C. Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
TABLE D.2-10. FATHEAD MINNOW BIOASSAY RESULTS0 FOR PLANT B112D
Time
24-Hour
4 8 -Hour
7 2 -Hour
9 6 -Hour
LC50 value,
% of neat effluent
70.0
69.2
69.2
65.7
95%
Confidence limits
_b
60.9 - 80.9
60.9 - 80.9
57.7 - 77.6
Assay performed by State Water Control Board,
Commonwealth of Virginia.
395% Confidence .limits not reported.
D.2-20
-------
D.2.3 Data Interpretation
D.2.3.1 Evaluation of Protocol Performance—
The wastewater effluent from Plant B112D exhibited moderate
toxicity to fathead minnows (LC50 = 65.7%, 96 hr). Based on the
inorganic and ionic analyses, potential sources of the toxicity
could be chromium concentration at 258 pg/L, copper at 32.6 pg/L,
and zinc at 234 pg/L. Even though the wastewater TOC was only
18 mg/L versus a decision point of 50 mg/L, GC/MS analyses were
initiated. A total of 2.78 mg/L of organic compounds were iden-
tified by GC/MS, but there is little evidence for toxicity due to
a specific compound, in fact, 0.344 mg/L of this was determined as
total phenol which would not be toxic to fathead minnows. The
bioaccumulation test results indicated 20 peaks with log P >3.5.
Several of these peaks were off-scale at relatively high concen-
trations. One of these was identified as pentachlorophenol.
D.2.3.2 Protocol Changes Suggested for Plant B112D—
No changes suggested.
D.2.3.3 Potential Sources of Chemical Toxicity—
Potential sources of the moderate toxicity noted in Plant B112D
wastewater effluent include chromium, copper, and zinc at con-
centrations of 255 [jg/L, 32.6 pg/L, and 234 pg/L, respectively.
An alternate candidate for toxicity could be chlorinated phenols
such as pentachlorophenol which is a major wood preservative
employed in the plant processes.
D.2-21
-------
D.3 PLANT B119D: PLASTICS MANUFACTURE
D.3.1 Process Description
Plant B119D is a manufacturer of molded primary plastic parts and
fabricated miscellaneous finished plastic products. Their primary
product is polyester film (SIC 3079).
Figure D.3-1 is a wastewater treatment diagram for the plant. No
actual wastewater treatment exists at the plant. The concentrated
wastewater is incinerated by a vendor, the sanitary waste is aero-
bically treated with activated sludge in a holding tank (15,000 GPD),
and the combined wastewater from sanitary, blowdown, and runoff is
treated with muriatic acid for pH control before final discharge.
The total plant effluent flow rate is about 50,000 GPD.
CONCENTRATED
WASTEWATER
INCINERATED
BY VENDOR
BLOWDOWN
COOLING WATER,
STEAM AND
SURFACE WATER
SANITARY
WASTE
ACTIVATED
SLUDGE
, t
CHLORINE
SAMPLE
COLLECTION
POINT
MURIATIC ACID
DISCHARGE
Figure D.3-1.
Was±e treatment diagram for plant B119D:
polyester film production.
D.3-1
-------
D.3.2 Analysis Results, Plant B119D
The following tables in this section present the data collected
for Plant B119D.
TABLE D.3-1. SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B119D
Temper- Dissolved
Flow, ature, oxygen,
m3/d (MGD) pH °C (°F) mg/L Comments
833a 7.4 21.5 9.0 Plant operating normally; effluent had
(0.22) (70.7) white surface foam.
alnstantaneous measurement.
TABLE D.3-2. WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B119D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD5
Total organic carbon
Total cyanide
Total phenol
Total suspended soilds
Total mercury, pg/L
Filtered mercury, pg/L
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
99
43
0.022
1.139
18.7
<0.20
<0.20
3.713
0.860
6.83
0.4
41.0
11.6
14.1
2.52
3.26
2.96
6
1.0
0.020
0.010
0.1
0.20
0.20
NR*
NR
0.04
0.25
0.5
1.0
2.5
0.007
NR
NR
aNo detection limit reported.
D.3-2
-------
TABLE D.3-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B119D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected
None detected
aBackground correction based on average of organic-
free water blanks.
Internal standards added to sample immediately
before purging.
D.3-3
-------
TABLE D.3-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
B119D CORRECTED FOR BACKGROUND '
O
•
w
i
Peak
No.
8
2
1
2
3
4,5
6
7
9
10
Retention
time,
min
21.08
6.60
3.38
6.60
11.88
14.8
15.20
17.35
22.22
29.90
Compound
^
Internal standard
Anthracene-d10
Recovery standard 'e
Phenol- d5
Organics detected 'g'
Unknown (83, 42, 69, 97)
Phenol
Ethanol, 2-phenoxy-
Benzoic acid, ethyl-
Unknown (148, 131, 103,
77)
Unknown (133, 104, 77)
Unknown (231, 77, 79,
232)
Phthalate
Peak area,
total counts
205,203
-
3,205
378,282
36,874
42,203
32,926
9,972
5,165
8,126
Amount Amount
detected, spiked. Recovery,
|jg/L ug/L percent
100
";
67.9 223 30.4
1.6
1551
18
21
16
4.9
2.5
4.0
(continued)
-------
TABLE D.3-4 (continued)
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked. Recovery,
total counts M9/L pg/L percent
Organics detected
(continued)
Total concentration
f,9,h
223
Background correction based on reagent and/or method blank analysis, Appendix 3.
Includes all substances that were found at or above a 2% threshold on the.BATCH peak
identification program in the Hewlett-Packard system and are greater than 1.6 pg/L
relative to the anthracene d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
oeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-d10,
internal standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Phenol and phenol-d5 co-elute. Amount detected is corrected for 15.7% phenol-d5
present.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.3-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT B119D CORRECTED FOR BACKGROUND '
RRI
201.87
_i
298.36
397.91
498.02
— ,
-
106.32
249.65
264.40
277.99
289.46
Peak
No.
6
1
4
11
13
14
2
3
5
7
8
9
10
Retention
time,
min
21.08
7.65
14.77
25.20
29.02
33.05
11.92
13.92
15.22
23.12
23.75
24.33
24.82
Compound
Internal standard
Anthracene-d10
Recovery standard '
1 , 2-Dichlorobenzene-d4
Biphenyl-djo
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '"
Ethanol, 2-phenoxy-
Decanal, o-methyloxime
[l,l'-Biphenyl]-2-ol and/or
benzene, l,l'-oxybis-
Oxacycloheptadecan-2-one
Bicyclohexyl, phenyl- (isomer)
Bicyclohexyl, phenyl- (isomer)
Benzene , 1,1' -cyclohexylidenebis-
( isomer)
Peak area,
total counts
169,723
125,266
155,989
99,971
56,014
35,916
81,387
11,943
13,715
5,651
10,666
5,703
11,931
Amount
detected,
pg/L
100
55.3
55.8
57.9
55.8
54
48.0
7.0
8.1
3.3
6.3
3.4
7.0
Amount
spiked, Recovery,
pg/L percent
-
118.1 46.8
101.6 54.9
99.3 58.3
83.0 67.2
85.0 63.5
(continued)
-------
TABLE D.3-5 (continued)
O
*
U)
RRI
301.30
Retention
Peak time ,
No . min
12 25.32
Compound
Organics detected '" (continued)
Benzene, l,l'-cyclohexylidenebis-
Total concentration
Amount
Peak area, detected,
total counts pg/L
4,682 2.8
85.9
Amount
spiked, Recovery,
pg/L percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 2.8 ug/L
relative to the anthracene d10 internal standard.
c
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 ug/L in original sample.
i>euterated recovery standard (spike), added to original effluent before extraction.
g
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
^Tentative identification, not confirmed with authentic standards.
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE D.3-6. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B119D
I
00
Concentration
in each fraction
(mg/L)
Extractable organics
Purgeable Base/
Analysis organics Acid neutral
TOC
GRAV 1
TCG 0
TCO .0
Sum of organics detected by GC/MS ND3'-5 0
Carbon balance
% Purgeable
% Extractable f
% Chromatographable
% GRAV chromatographable5,
% Extractables identified
Protocol identification efficiency , %
.80 4.68
.15 0.25
.54 0.14
.22D 0.08
Total
43
6.50
0.40
0.68
0.30
ND
16.7
2.5
6.2
0.7
0.7
aFrom Table D.3-3. From Table D.3-4.
'V of carbon tmracablc - Total mass of P^rgeables 1QO
/o *->JL OclI-JJvJIi puiy truIJ J_c — Tor1 A AV/U
61 r» - 7!P^. +..^^^^ v 1 ftn
/4) »JJ. ^CIJ.JJ(JI1 tJX H. cl<- L.CUJ -L tJ *- TOP J.UU
y nf far'hnn fl-iT-ntnni-rkrfrTnVnKI » — ^-**^ ™**5 v 1 OO
"% of grav chromatographable = _. v x 100
N nf nvl-r-nntnhlnr. i rtonlH f i nrt ^ Total mass of acid GC/MS
°From Table D
+ B/N GC/MS
.3-5.
n
X wW
1Protocol organics identification efficiency summartion of Equations d and h.
indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.3-7. ICAP METALS ANALYZED BY EPA REGION V,
PLANT B119D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
3.08
96
<80
32.7
<1.0
14,000
<2.0
<6.0
35.6
63.2
806
<10.0
5,000
3,500
67.1
<10.0
55,000
69.5
<70
158
83.0
<25
<5.0
<5.0
301
aData not reported.
D.3-9
-------
TABLE D.3-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B119D EFFLUENT
to
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
t , min Log t
r' y r
2.00
2.11
3.09
3.39
3.54
4.57
5.74
6.11
6.25
7.65
8.31
9.85
11.25
12.70
14.53
15.70
18.03
20.00
21.63
26.26
28.75
0.3010
0.3243
0.4900
0.5301
0.5490
0.6599
0.7589
0.7860
0.7959
0.8837
0.9196
0.9934
1.0512
1.1038
1.1623
1.1959
1.2560
1.3010
1.3351
1.4191
1.4586
Calcd.
log P
0.37
0.49
1.35
1.56
1.66
2.23
2.75
2.89
2.94
3.39
3.58
3.96
4.26
4.54
4.84
5.02
5.33
5.56
5.74
6.18
6.38
Peak
height,
cm
>15
>15
>15
>15
>15
>15
4.7
12.7
11.9
3.1
10.0
13.7
1.1
0.8
0.7
0.5
0.3
0.3
0.4
0.2
0.1
Adjusted
peak
height,
cm
>1,300
>1,300
>1,300
>1,300
>1,300
>1,300
400
1,100
1,000
270
860
1,200
95
69
60
43
26
26
34
17
9
Estimated
, concentration Positive
FSD, index, or
% ppb negative
>8,600d
>8,600
>8,600
>8,600
>8,600
>8,600
2,700
7,300
6,800
1,800
5,700
7,900
630
460
400
290
170
170
230
110
57
>340 •
>340
>340
>340
>340
>340
110
ISO6
270
71
230 +
310 +
25 +
18 +
16 +
11 +
7 +
7 +
9 +
6 +
2 +
(continued)
-------
TABLE D.3-8 (continued)
O
•
u>
Peak
number
22
23
24
t , min
31.13
34.34
39.13
Log tr
1.4932
1.5358
1.5925
Calcd.
log P
6.56
6.78
7.08
Peak
height,
cm
0.1
1.1
0.6
Adjusted
peak
height,
cm
9
95
52
K
FSD,D
%
57
630
340
Estimated
concentration Positive
index, or
ppb negative
2 +
25 +
14 +
Retention time.
Percent full-scale deflection.
••
"A positive response is defined as log P =£3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.3-9.
CORRELATION3 OF BIOACCUMULATION POTENTIALb AND
GC/MS ANALYSIS TEST RESULTS: PLANT B119D
Organic compounds identified by Molecular
GC/MS analysis formula
(Biphenyl)c C12H10
Actual sample
Literature log P values
log P value [1] by HPLC method
3.58
3.95 3.96
4.26
4.54
4.84
5.02
5.33
5.56
5.74
6.18
6.38
6.56
6.78
7.08
Decananl, o-methyloxime CnH23NO
[l,l'-Biphenyl]-2-ol C12H100
Benzene, l,l'-oxybis- Ci2Hio°
Oxacyloheptadecan-2-one Ci6H30°2
Bicyclohexyl, phenyl- C18H26
Benzene, l,l'-cyclo-
hexylidenebis- Ci8H20
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
§3.5 and ^25% FSD).
CTentative identification based solely on log P values and similar compounds
in sample.
NO literature log P found.
[1] Hausch, C. Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
D.3-12
-------
TABLE D.3-10. CALCULATED LC50'S FOR MYSID SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B119D IN
STATIC, UNAERATED ARTIFICIAL SEAWATERa
LC50,95% confidence limits,
Hour % effluent % effluent
24
48
72
96
>100%
>100%
>100%
>100%
"K
*"K
Note: Salinity was 24 o/oo and temperature
was 22°C. Values were based on
nominal concentrations of test
material in artificial seawater.
Dissolved oxygen and pH remained
within acceptable ranges through-
out the test.
aAssay was performed by EG&G Bionomics,
Pensacola, Florida.
Confidence limits not calculated due to
LC50
TABLE D.3-11. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT B119D
LC50 value,95%
Time % of neat effluent Confidence limits
24 -Hour
48 -Hour
7 2 -Hour
9 6 -Hour
30.5
19.2
17.5
17.5
_b
-.
-r'
—
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
95% Confidence limits not reported.
D.3-13
-------
D.3.3 Data Interpretation
D.3.3.1 Evaluation of Protocol Performance—
The wastewater effluent from Plant B119D was nontoxic to mysid
shrimp, but was moderately toxic to fathead minnows (LC50 = 17.5%,
96 hr)'. The inorganic and ionic analyses indicated a concentration
of 63.2 mg/L of copper in the wastewater which could account for
the toxicity. Although no free chlorine was reported, the waste-
water treatment process employed included chlorination. Phenol
was observed at 1.139 mg/L, but this concentration is not consid-
ered to be toxic to the test species. Even though the TOC of
43 mg/L did not exceed the protocol decision point of 50 mg/L,
GC/MS analyses were conducted. Unfortunately, only 0.7% of the
total organic content was readily identified and therefore no con-
clusions can be drawn concerning the toxicity of the organic mate-
rial in the effluent. Although 14 bioaccumulative peaks were found
those that were identified could not explain the toxicity of the
effluent.
D.3.3.2 Protocol Changes Suggested for Plant B119D—
No changes in the protocol are suggested.
D.3 .3.3 Potential Sources of Chemical Toxicity—
The only source of chemical toxicity observed was a concentration
of 63.2 pg/L of copper in the wastewater effluent. Although no
free chlorine analysis was reported, it is suspected that the
difference in toxicity of the effluent to mysid shrimp and to fat-
head minnows can be attributed to aeration of the effluent, which
would have removed free chlorine, before the test on mysid shrimp
was performed. Aeration was not performed on the effluent used
for the fathead minnows. For all effluent biological tests, the
effluents are aerated prior to mysid shrimp tests but not prior
to fathead minnow tests.
D.3-14
-------
D.4 PLANT C150D: MUNICIPAL SEWAGE TREATMENT PLANT
D.4.1 Process Description
Plant CISOD.is a combined municipal/industrial sewage treatment
plant (SIC 4952). Figure D.4-1 is a diagram of the unit opera-
tions of the primary sewage treatment plant. Influent (2.1 MGD)
has alum added to it, is screened, goes through a grit chamber,
has a polymer added to it before entering a primary settling tank,
and is chlorinated before final discharge. The sludge from the
primary sedimentation is anaerobically digested. Hydraulic reten-
tion time through the plant is about 1.15 hours.
INFLUENT
SCREENING
GRIT
REMOVAL
PRIMARY
SEDIMENTATION
ANAEROBIC
SLUDGE
DIGESTION
1 SAMPLING
V POINT
J
Figure D.4-1.
EFFLUENT
Waste treatment diagram for Plant C150D:
municipal sewage treatment.
D.4-1
-------
D.4.2 Analysis Results, Plant C150D
The following tables in this section present the data collected
for Plant C150D.
TABLE D.4-1.
SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C150D
Flow,
m3/d (MGD)
PH
Temper- Dissolved
ature, oxygen,
°C (°F) mg/L
Comments
9,270 6.3 19.0 2.7 Effluent chlorine content was 2.06 mg/L.
(2.45) (66.2) Effluent was aerated from time it was
brought to lab to decrease chlorine
content; total chlorine in 100% tank at
beginning of bioassay was 0.07 mg/L.
All tanks were aerated throughout the
bioassay due to high oxygen demand.
Primary plant; plant operating normal-
ly; effleunt gray in color and had
sewage odor.
Instantaneous measurement.
TABLE D.4-2.
WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C150D
(mg/L, except as noted)
Species
Concentration
Detection
limit
BOD5
Total organic carbon
Total cyanide
Total phenol
Total suspended soilds
Total mercury, pg/L
Filtered mercury, ug/L
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
92.3
55
<0.020
0.066
42
0.20
<0.20
46.8
47.4
<0.04
6
1.0
0.020
0.010
0.1
0.20
0.20,
NRb
NR
0.04
(continued)
D.4-2
-------
TABLE D.4-2 (continued)
Species
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Concentration
0.8
165
79
<25
2.34
4.64
2.26
Detection
limit
0.25
0.5
1^0
25
0.007
NR
NR
aSamples exceeded required holding times.
No detection limit reported.
TABLE D.4-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C150D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected0
4.0 Methylchloride 15,
15.1 Chloroform
-------
TABLE D.4-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
C150D CORRECTED FOR BACKGROUND '
p
I
Peak
No.
19
1
2
3
4
5
6-8
9
10
11-12
13
14-16
Retention
time,
min
21.10
6.50
8.82
9.18
11.35
11.57
12.83
13.55
14.23
14.88
16.72
18.07
Compound
Internal standard
Anthracene-d10
Recovery standard '
Phenol- d5
Organics detected '"'
Phenol, methyl-
(isomer)
Phenol, 2-chloro-6-
methyl-
Unknown (122,105,77,73)
Unknown (73, 50, 101,
105)
Benzeneacetic acid
Benzoic acid,
2-hydroxy-
Benzenepropanoic acid
Unknown (129, 73, 55)
Unknown
Unknown acid (73, 129,
157, 200)
Peak area,
total counts
198,023
34,603
72,009
4,795
94,316
31,979
68,381
7,557
19,668
40,693
8,694
155,853
Amount Amount
detected, spiked, Recovery,
pg/L ug/L percent
100
.
36.9 201 18.4
36.4
2.4
47.6
16.2
34.5
3.8
9.9
20.6
4.4
78.7
(continued)
-------
TABLE D.4-4 (continued)
o
in
Peak
No.
17
18
20
21
22
23
24a
24b
24c
25
26
27
Retention
time,
min
20.80
20.98
21.93
23.12
23.88
24.30
25.50
25.50
25.60
25.77
28.38
29.90
Compound
Organics detectedf /g/h
(continued)
Unknown acid (73, 129,
185, 228)
Unknown (150, 224, 54,
96)
lH-Purine-2 , 6-dione ,
3,7-dihydro-l,3,7-
trimethyl-
Unknown (69, 55, 97,
236)
Unknown acid (73, 129,
256, 213)
Sulfur, mol. (S8)
Unknown (69, 81, 67,
73)
Unknown (97, 83, 69,
55, 264)
Unknown (69, 83,
97, 67)
Unknown (73, 129,
284, 185)
Hexanedioic acid,
dioctyl ester1
Phthalate
Peak area,
total counts
40,986
5 , 594
7,172
i
7,410
41,329
28,594
3,615
15,152
7,723
13,124
59,821
4,270
Amount Amount
detected, spiked, Recovery,
|jg/L ug/L percent
20.7
2.8
3.6
3.7
20.9
14.4
1.8
7.6
3.9
6.6
30.2
2.2
(continued)
-------
TABLE D.4-4 (continued)
Peak
No.
Retention
time,
min
Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pg/L pg/L percent
Organics detected 'g/
(continued) '
28 35.83 Unknown (silicon-
containing) (207,
281, 208)
Total concentration3
5,466
2.8
376
O
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 1.8 |jg/L
relative to the anthracene d10 internal standard.
p
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
oeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-d10,
internal standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to
decreasing intensity. Possible molecular ions are underlined.
Possible contaminant introduced during sample workup,- also present in method blank.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.4-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT C150D CORRECTED FOR BACKGROUND '
o
RRI
202.34
J
-
298.36
397.91
498.02
_
-
-
Peak
No.
19
3
12
25
26
27
1
2
4
Retention
time,
min
21.10
7.65
14.75
25.20
29.02
33.05
7.13
7.52
9.63
Compound
Internal standard
Anthracene-d10
Recovery standard '
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf 'g'h
Benzene, dichloro- (isomer)
Benzene, 1 -methyl- (1-methylethyl) -
(isomer) and/ or benzene, ethyl-
dimethyl- (isomer)
Benzeneethanol
Peak area,
total counts
150,873
112,618
51.2
120,354
44.0
71,308
44.2
28,112
30.8
19,732
32.1
8,545
5,726
15,702
Amount
detected,
pg/L
100
63.8
47.7 .
38.3
30.5
28.8
5.7
3.8
10.4
Amount
spiked, Recovery,
Mg/L percent
-
118.1 54.0
43.4
101.6 46.9
43.3
99.3 38.6
44.5
85.8 35.5
35.9
90.6 31.8
35.4
(continued)
-------
TABLE D.4-5 (continued)
RRI
_
-
-
-
-
-
-
121.07
125.93
151.70
157.37
176.01
221.78
230.91
Peak
No.
5
6
7
8
9
10
11
13
14
16
17
18
20
21
Retention
.time. Peak area,
min Compound total counts
10.10
10.30
10.75
10.92
11.30
11.75
13.52
16.13
16.43
18.02
18.37
19.52
21.93
22.32
Organics detected '"' (continued)
Unknown (81, 136, 121, 107)
Unknown (59, 95, 81, 123, 152)
Bicyclo[2.2.1]heptan-2-ol, 1,7,7-
trimethyl-,
Cyclohexanol , 5-methyl-2-(l-methyl-
ethyl)-, lot,2B- (isomer)
3-Cyclohexene-l-methanol, a,o,4-
trimethyl-, (S)-
Sulfur-containing Unknown (158,
79, 160)
Unknown (71, 59, 109, 108,
137)
Unknown (129, 111, 171)
Unknown alcohol or unsaturated
hydrocarbon (69, 83, 97, 111)
Unknown alcohol or unsaturated
hydrocarbon (97, 69, 83, 70)
Unknown Phthalate
Unknown alcohol or unsaturated
hydrocarbon (97, 83, 69, 55)
IH-Purine-l , 6-dione , 3 , 7-dihydro-
1,3,7-trimethyl-
Unknown alcohol or unsaturated
hydrocarbon (83, 97, 69, 57, 111)
6,265
28,474
14,420
11,725
58,271
8,483
7 , 540
5,294
16,201
4,662
19,019
6,327
13,806
7,316
Amount Amount
detected, spiked, Recovery,
|jg/L pg/L percent
4.1
18.8
9.6
7.8
38.6
5.6
5.0
3.5
10.7
3.1
12.6
4.2
9.1
4.8
(continued)
-------
TABLE D.4-5 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
Mg/L
Amount
spiked,
pg/L
Recovery,
percent
vD
Organics detected 'g/ (continued)
238.17 22
255.50 23
280.80 24
548.76 28
22.63 Cl-containing unknown (228, 140,
. 183, 220)
23.37 Unknown (73, 129, 179, 256)
24.45 Sulfur, mol. (S8)
35.30 Unknown (215, 207, 233, 373,
388, 355)
Total concentration1
4,406
3,576
533,667
18,689
2.9
2.4
354
12.4
529
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 2.4 |jg/L
relative to the anthracene d10 internal standard.
Q
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 |jg/L in original sample.
oeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-d10,
internal standard.
tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE D.4-6. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT Cl5OD
Analysis
Concentration in each fraction (mg/L)
Extractable organics
Purgeable Base/
organics Acid neutral Total
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
<0.05
2.40
0.54
0.65,
0.38r
3.90
0.52
0.40
0.53*
55
6.30
1.06
1.05
<0.96
Carbon balance
% Purgeable
% Extractable f
% Chromatographable
% GRAV Chromatographable9,
% Extractables identified .
Protocol identification efficiency , %
13.4
3.8
16.8
1.7
From Table D.4-3.
From Table D.4-4.
"From Table D.4-5.
of carbon purgeable = Total massif purgeables x 1Q()
e% of carbon extractable = TCO * GRAV x 100
X VJ\*
% of carbon Chromatographable =
x 100
o
y
% of grav Chromatographable =
x 100
\ of extractables identified = Total mass of acidGC/MS + B/N GC/MS x 1Q<)
Protocol organics identification efficiency summartion of Equations d and h.
-------
TABLE D.4-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C150D, jjg/L
Unfiltered Suspended
Compound effluent solids
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
7
3,450
794
10.
<1
15,300
<2
<6
210
48.
1,520
<10
16,100
6,200
66
12.
131,000
120
94.
124
73
<25
<5
<5
58
5
3
5
7
not reported.
D.4-11
-------
TABLE D.4-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C150D EFFLUENT
o
*
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
t , min
1.96
2.50
3.40
3.68
3.90
4.84
5.36
5.89
6.27
6.74
7.77
8.77
9.36
10.50
12.00
12.53
15.18
16.30
17.81
19.20
Log tr
0.2923
0.3979
0.5315
0.5658
0.5911
0.6848
0.7292
0.7701
0.7973
0.8287
0.8904
0.9430
0.9713
1.0212
1.0792
1.0980
1.1813
1.2122
1.2350
1.2833
Calcd.
log P
0.39
0.89
1.52
1.69
1.81
2.25
2.46
2.66
2.79
2.93
3.23
3.48
3.61
3.85
4.12
4.21
4.61
4.76
4.87
5.09
Peak
height,
cm
2.0
1.3
12.7
>15.0
>15.0
6.0
4.0
2.5
3.1
8.5 -
1.6
8.5
8.3
0.8
1.2
8.0
0.4
0.5
1.0
0.3
Adjusted
peak
height,
cm
180
120
1,200
>1,400
>1,400
550
360
230
280
770
150
770
760
73
110
730
36
46
91
27
Estimated
. concentration
FSD, index,
% ppb
l,200d
790
7,700
>9,100
>9,100
3,600
2,400
1 , 500
1,900
5,200
970
5,200
5,000
490
730
4,900
240
300
610
180
476
32
310
>360
>314C
150 .
97
61
75
50C
39
210
50C
19
29
190
10
12
24
7
Positive
°r c
negative
_
-
-
-
-
-
-
-
- ' •
-
-
_
+
+
+
+
+
+
+
+
(continued)
-------
TABLE D.4-8 (continued)
0
It*
i
H
(jO
Peak
number
21
22
23
24
25
26
t , min
20.43
23.50
25.69
34.05
43.82
50.68
Log tr
1.3103
1.3711
1.4098
1.5321
1.6417
1.7048
Calcd.
log P
5.22
5.51
5.70
6.28
6.80
7.10
Peak
height,
cm
0.3
0.4
1.8
0.3
»15.0
0.4
Adjusted
peak
height.
cm
27
36
160
27
»1,400
36
K
FSD,
%
180
240
1,100
180
»9,100
300
Estimated
concentration Positive
index ,
ppb
7
10
44
7
»360
12
or
negative
+
+
+
+
+
+
Retention time.
Percent full-scale deflection.
r*
"A positive response is defined as log P ^3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
a
"Corrected for background interference.
-------
TABLE D.4-9.
CORRELATION3 OF BIOACCUMULATION POTENTIAL1* AND
GC/MS ANALYSIS TEST RESULTS: PLANT C150D
Organic compounds identified by
GC/MS analysis
Dichlorobenzene
(Pentachlorocyclohexene)6
( 1 , 2 , 3-Trichlorobenzene )e
(2 ,4-Dichlorotoluenee
(1 ,2,3,4-Tetrachlorobenzene)e
(Pentachlorophenol ) e
(Hexachlbrobenzene )
2-Chloro-6-methyl-phenol
Benzeneacetic acid
Benzenepropanoic acid
lH-Purine-2,6-dione, 3,7,-
dihydro- 1,3,7 -trimethyl-
Hexanedioic acid, dioctyl ester
l-Methyl-(l-methylethyl)-
benzene
Ethyl-dimethyl-benzene
Benzeneethanol
1,7,7-Trimethyl-, bicyclo-
[2.2.1]heptan-2-ol
Cyclohexanol, 5-methyl-2-(l-
methylethyl)-,(la,2B,5B)-
3-Cyclohexene-l-methanol,
ot,a,4-trimethyl-, (S)^
Molecular
formula
C6H4C12
CeHsCls
C6H3C13
C7H6C12
C6H2C14
C6HC150
C6C16
C7H7C10
C8Hg02
C9H1002
C8H10N402
C22H42°4
C10H14
C10H14
C8H100
C10H18°
C10H20°
C10H18°
Literature
log P value [1]
3.62C'd
3.85
4.11
4.24
4.64
5.12
6.27
1
-
-
- •
-
-
-
-
-
-
"
Actual sample
log P values
by HPLC method
3.61d
3.85
4.12
4.21
4.61
4.76
4.87
5.09
5.22
5.51
5.70
6.28
6.80
7.10
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
£3.5 and £25% FSD).
C0ther values reported are 3.38, 3.39, 3.55, 3.60.
t'otential correlation.
Tentative identification based solely on log P values and similar
compounds found in sample.
No literature log P found.
[1] Hausch, C. Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
D.4-14
-------
TABLE D.4-10. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT C150D
Time
24-Hour
48-Hour
72-Hour
9 6 -Hour
LC50 value,
% of neat effluent
56.4
49.0
47.2
44.7
Q^y
~->A>
Confidence
48.2 -
41.1 -
28.8 -
27.8 -
limits
6.7.8
60.0
98.2
88.3
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
D.4.3 Data Interpretation
D.4.3.1 Evaluation of Protocol Performance—
The wastewater effluent from Plant C150D was found to be of
moderate to low toxicity to fathead minnows (LC50 = 44.7%; 96 hr).
The inorganic and ionic analyses indicated three possible sources
for this toxicity, including a chromium content of 210 [ig/l>, and
a copper content of 48.3 H9/L. Since the TOC value of 55 mg/L
exceeded the protocol decision point, the wastewater was subjected
to GC/MS analyses. The purgeable, acid, and base-neutral extracts
totaled less than 1 mg/L and showed mo major cause for the toxicity
from an organic chemical view point. However, the bioaccumulative
peaks numbered 14, with one much greater than 360 ppb. This peak,
however, was not identified.
D.4.3.2 Protocol Changes Suggested for Plant C150D—
The unidentified bioaccumulative compound peak should have been
isolated and specific GC/MS identification conducted.
D.4.3.3 Potential Sources of Chemical Toxicity—
Potential sources of the toxicity observed of the wastewater
effluent from plant C150D include free chlorine and chromium,
copper and zinc contents of 210 ug/L, 48.3 \ig/L and 58.3 H9/L/
respectively.
D.4-15
-------
D.5 PLANT C155D; MUNICIPAL SEWAGE TREATMENT PLANT
D.5.1 Process Description
Plant C155D is a combined municipal/industrial sewage treatment
plant (SIC 4952). Figure D.5-1 is a wastewater treatment diagram
for the primary treatment plant. Influent is first screened, then
alum is added for coagulation, followed by grit removal, polymer
addition for flocculation, primary settling (flotation and con-
ventional), chlorination for disinfection, and final discharge.
The sludge from the sedimentation tanks goes to a sludge holding
tank, is thickened, then incinerated. Hydraulic retention time
through the plant is estimated at 2.3 hours. The total plant
effluent flow rate is 18.78 MGD.
GRIT TO
DISPOSAL
SEDIMENTATION
TANKS
BYPASS LINE
—CI2
SAMPLING
POINT
DISCHARGE
INCINERATOR
SLUDGE
THICKENING
1
SLUDGE
HOLDING
TANK
ASH TO
DISPOSAL
Figure D/5-1.
Wastewater treatment diagram for plant
C155D: municipal sewage treatment.
D.5-1
-------
D.5.2 Analysis Results, Plant C155D
The following tables in this section present the data collected
for Plant C155D.
TABLE D.5-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C155D
Temper- Dissolved
Flow, ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments
71,9l5a
(19)
6.5 19.5
(67.1)
NR Chlorine = 1.77 ppm,- plant in process of
being upgraded from primary to full
secondary; effluent grayish in color.
a
Instantaneous measurement.
No reading taken.
TABLE D.5-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C155D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
72
35
<0.020
0.053
25.3
<0.20
<0.20
24.12
23.4
<0.04
0.5
360
80
<250
0.616
1.13
0.577
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
250
0.007
NR
NR
detection limit reported.
D.5-2
-------
TABLE D.5-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C155D, CORRECTED FOR BACKGROUND
Retention
time , min
13.9
25.6
10.5
15.3
15.1
17.1
21.2
25.9
. 27.5
•• 30.1
, 32-33
Concentration ,
Compound • p g/L
Internal Standards
Bromochl or ome thane
1 , 4-Dichlorobutane
Organics Detected0
Methylene chloride
1 , 2-Trans-dichloroethylene
Chloroform
1,1, 1-trichloroethane
Benzene
Tetrachloroethylene
Toluene
Ethylbenzene
Xylenes
400
400
<10d
<10d
28d
-------
TABLE D.5-4
ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
C155D CORRECTED FOR BACKGROUND '
ui
i
Peak
No.
11
2
1
3
4a
4b
5
6
7
8
9
10
12
Retention
time. Peak area,
min Compound total counts
21.10
6.52
4.60
8.45
11.3
11.4
13.48
14.18
14.75
17.08
17.85
20.72
21.93
Q
Internal standard
Anthracene-d10
Recovery standard 'e
Phenol-d5
Organics detected '"'
Unknown (MW = 100)
(57,87,41,45)
Unknown (possibly 1,2-
cyclohexanediol MW =
116) (70,98,83,56)
Unknown (72,57,102,87)
Unknown (73,60,101,85)
Benzoic acid, 2-hydroxy
Benzenepropanoic acid
Unknown (73,129,164,60)
(l,l'-Biphenyl)-2-ol
Unknown (73,129,157)
Unknown (73,129,185,60)
IH-pur ine-2 , 6-dione ,3,7-
dihydro-l,3,7-trimethyl
223,045
61,614
2,756
10,332
7,249
54,858
6,682
15,834
13,762
10,493
5,648
4,014
8,682
Amount Amount
detected, spiked, Recovery,
|jg/L |jg/L percent
100
60.9 201 30.3
1.2
4.6
3.2
25
3.0
7.1
6.2
4.7
2.5
1.8
3.9
(continued)
-------
TABLE D.5-4 (continued)
p
in
i
en
Peak
No.
Retention
time,
min
Compound
Peak
total
Amount
area, detected,
counts Mg/L
Amount
spiked,
pg/L
Recovery,
percent
Organics detected
(continued)
13
14a
14b
22.60 Cl-containing unknown
(MW = 208) (218, 183,
140,165)
23.3 Unknown (73,129,256,213)
23.4 Unknown (149,354,188,
150)
Total concentration1
3,697
6,729
2,744
1.7
3.0
1.2
69.1
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 1.2 ug/L
relative to the anthracene-d10 internal standard.
anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 ug/L in original sample.
i)euterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-
d10, internal standard.
"Tentative identification, not confirmed with authentic standards.
masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.5-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT C155D CORRECTED FOR BACKGROUND '
Peak
RRI No.
202.34 15
-j 5
13
? 298.83 18
w 398.17 20
o> 498.51 21
1
2
3
Retention
time,
min
21.10
7.67
14.77
25.22
29.03
33.07
5.90
6.52
6.72
Compound
Internal standa"rdc
Anthracene-d10
d e
Recovery standard '
1,2, -dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected 'g'
Benzene, ethyl methyl-
(isomer)
Benzonitrile and/or
benzene , isocyano
Benzene-, trimethyl-
(isomer) and/ or
Peak area.
total counts
145,743
176,945
223,888
120,702
55,080
42,487
20,536
9,384
22,252
Amount
detected,
pg/L
100
100.6
95.5
72.3
66.1
66.3
14.1
6.4
15.3
Amount
spiked, Recovery,
pg/L percent
. .-
118.1 85.2
101.6 94.0
99.3 72.8
85.8 77.0
90.6 73.2
benzene, ethyl methyl-
(isomer)
7.13 Benzene, dichloro-
(isomer)
15,478
10.6
(continued)
-------
TABLE D.5-5 (continued)
in
i
RRI
_
-
-
-
-
-
-
157.37
221.78
277.28
386.94
Peak
No.
6
7
8
9
10
11
12
14
16
17
19
Retention
time,
mi'n
9.63
10.30
10.75
10.92
11.32
13.25
13.40
18.37
21.93
24.30
28.60
Compound
Organics detectedf 'g/h
(continued)
3-Thujen-2-ol (isomer)
and/or benzeneethanol
and/ or benzene, butyl
Unknown (59, 81, 95, 152)
Bicyclo[2.2.1]heptan-2-ol,
1,7,7-trimethyl-, endo-
( isomer)
Cyclohexanol, methyl-
(methylethyl)- (isomer)
3 - Cyc lohexene - 1 -me thanol ,
of,a,4-trimethyl-, (S)
Naphthalene, me thy 1-
( isomer)
Unknown hydrocarbon (85,
71, 57, 43, 184)
Phthalate (149, 177, 150,
176)
lH-Purine-2,6-dione, 3,7-
dihydro-1 , 3 , 7- trimethyl
Sulfur, mol. (S8)
Unknown (125, 199, 85, 227,
57)
Peak area,
total counts
17,374
25,268
15,569
7,424
76,856
9,834
9,544
11,217
9,855
44,345
7,533
Amount Amount
detected, spiked, Recovery,
pg/L MQ/L percent
'
11.9
17.3
10.7
5.1
52.7
6.7
6.5
7.7
6.8
30.4
5.2
(continued)
-------
TABLE D.5-5 (continued)
RRI
Peak
No.
Retention
time,
min
Compound
Peak
total
area,
counts
Amount
detected,
pg/L
Amount
spiked,
Mg/L
Recovery,
percent
560.67 22
35.83
Organics detected
(continued)
f,g,h
Unknown (207, 386, 281,
105)
Total concentration
12,036
8.3
216
O
•
tn
oo
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 5.1 jjg/L
relative to the anthracene-d10 internal standard.
Q
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 (jg/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
eQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-
d10, internal standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
]Retention time less than biphenyl.
-------
TABLE D.5-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C155D
in
Concentration in each fraction (mg/L)
Extractable organics
Purgeable Base/
Analysis organics Acid neutral Total
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeable
% Extractable f
% Chromatographable
% GRAV chromatographable5.
% Extractables identified
35
1.80 3.25 5.00
0.25 0.49 0.74
0.35. 0.84 1.19
<0.11a 0.07D 0.22C <0.40
<0.3
17.7
5.5
14.8
<0.8
From Table D.5-3.
From Table D.5-4.
°From Table D.5-5.
*% of carbon purgeable = Total mass of purgeables x 10Q
JL \J\*
e% of carbon extractable = TOC * GRAV x 100
JL \J w
% of carbon chromatographable =
TCG
x 100
g% of GRAV chromatographable = GRAV x 10°
^ of extractables identified = Total mass of acid GC/MS + B/N GC/MS x 1QO
Protocol organics identification efficiency summation of Equations d and h.
-------
TABLE D.5-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C155D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3
1,280
259
15.3
<1
35,700
<2
<6
18
14.9
740
<10
21,200
27,800
64
<10
218,000
36
70
114
272
<25
<5
<5
41
aData not reported.
D.5-10
-------
TABLE D.5-8 BIOACCUMULATION POTENTIAL TEST RESULTS, PLANT C155D EFFLUENT
O
•
(n
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
t , min
1.38
1.97
2.38
3.69
3.91
4.88
5.90
6.36
6.75
7.43
7.76
8.79
9.39
10.44
11.25
12.25
13.08
13.88
14.75
17.62
19.50
26.00
44.08
Log tr
0.1399
0.2945
0.3766
0..5670
0.5922
0.6884
0.7709
0.8035
0.8293
0.8710
0.8899
0.9440
0.9727
1.0187
1.0512
1.0881
1.1166
1 . 1424
1.1688
1 . 2460
1.2900
1.4150
1.6442
Calcd. .
log P
-0.35
0.39
0.78
1.69
1.81
2.26
2.66
2.81
2.93
3.13
3.22
3.48
3.62
3.83
3.99
4.16
4.30
4.42
4.55
4.92
5.13
5.72
6.81
Peak
height,
cm
0.15
1.4
1.2
^15
>15
7.8
2.8
3.4
9.1
2.0
1.9
2.4
8.3
1.4
0.5
0.5
0.7
0.4
0.4
0.4
0.2
0.15
2.4
Adjusted
peak
height,
cm
14
130
110
>1,400
>1,400
710
260
310
830
180
170
220
760
130
46
46
64
36
36
36
18
14
220
Estimated
, concentration Positive
FSD, index, or
% ppb negative
91
850
730
>9,100
>9,100
4,700
1,700
2,100
5,500
1,200
1,200
1,500
5,000
850
300
300
430
240
240
240
120
91
1,500
4
34
29
>360
>3146
190
68
83
60e - |
49
46 -
536
506
34 +
12 +
12 +
17 +
10 +
10 +
10 +
5 +
. 4 +
58 +
Retention time.
Percent full-scale deflection.
"A positive response is defined as log P §3.5 with % FSD >25.
3
See Appendic C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.5-9. CORRELATION3 OF BIOACCUMULATION POTENTIAL13 AND
GC/MS ANALYSIS TEST RESULTS: PLANT C155D
Organic compounds identified by Molecular
GC/MS analysis formula
Literature
log P value [1]
Actual sample
log P values
by HPLC method
Dichlorobenzene
Methylnaphthalene
(1,2, 3-Trichlorobenzene ) e
(1,2, 4-Trichlorobenzene )
Butylbenzene
( 1 , 3-Dimethylnaphthalene )
(1 ,2,4,5-Tetrachlorobenzene)
1 ,2-trans-Dichloroethylene
Ethyl methylbenzene
Benzenepropanoic acid
[l,l'-Biphenyl]-2-ol
lH-Purine-2,6-dione, 3-7-
dihydro-1 ,3,7-trimethyl-
Isocyanobenzene
3-Thujen-2-ol
Benzeneethanol
1 ,7,7-Trimethyl- , endo-bicyclo-
[2.2.1]heptan-2-ol
Methyl- (me thylethyl)-cyclo-
hexanol
3-cyclohexene-l-methanol,a,or,4-
trimethyl-, (S)
C6H4C12
C11H10
C6H3C13
C12H12
C6H2C14
C2H2C12
C9H12
C9H1002
C12H100
C8H10N402
C7H5N
ClOH16°
C8H100
C10H180
C10H20°
C10Hi80
3.62Cd'd
3.86°
3.99
4.12
4.26d
4.42
4.52
-
-
-
-
-
-
- •
-
-
™
3.62*
3.83°
3.99
4.16
4.30°
4.42
4.55
4.92
5.13
5.72
6.81
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
S3.5 and §25% FSD).
C0ther values reported are 3.38, 3.39, 3.55, 3.60.
Potential correlation.
Tentative identification based solely on log P values and similar
compounds found in sample.
No literature log P found.
[1] Hausch, C. Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
D.5-12
-------
TABLE D.5-10.
CALCULATED LC50'S FOR MYSID .SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE C155D IN
STATIC, UNAERATED ARTIFICIAL SEAWATER
Hour
24
48
72
96
LC50 ,
% effluent
71
50
22
15
95% confidence limits,
% effluent
50-100
25-100
15-32
10-22
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based on
nominal concentrations of test
material in artificial seawater.
pH remained within acceptable range
throughout the test. Low dissolved
oxygen in 50% and 100% effluents
would appear to be the major cause
of mortality at these concentrations.
aAssay performed by EG&G Bionomics,
Pensacola, Florida.
D.5.3 Data Interpretation
D.5.3.1 Evaluation of Protocol Performance—
Plant C155D produced an effluent with moderate to high toxicity,
i.e., 15% of effluent produced a 96 hr LC50 for mysid shrimp.
The protocol revealed no significant contribution from anionic
compounds or trace metals and thus specified more extensive
organic analysis. Bioaccumulation screening revealed a substan-
tial number of hydropholic compounds, however most were estimated
to be below a concentration of 12 ppb. Most significant, though,
was the measured chlorine concentration of 1.77 mg/L. If the
protocol had been followed directly, no specific source of
toxicity would have been identified. However, GC/MS was pursued
even though the TOC value was less than the 50 mg/L limit cut-off.
D.5-13
-------
D.5.3.2 Protocol Changes Suggested for Plant C155D—
In cases where the plant effluent is chlorinated in the waste
treatment process, the sample is aerated before toxicity testing.
Therefore, it is not clear whether the toxicity could or could not
be due to residual free chlorine. It is suggested that free chlo-
rine tests be conducted on the aerated sample prior to aquatic
toxicity testing.
D.5.3.3 Potential Sources of Chemical Toxicity—
For Plant C155D effluent, the primary source of toxicity is pre-
sumed to be free chlorine from the wastewater treatment process.
Other secondary sources of toxicity could be ammonia at the
23.4 mg/L level, zinc at 41 |jg/L, and miscellaneous chlorinated
organics which were detected in purgeable and extractable
fractions.
D.5-14
-------
D.6 PLANT C156D: MUNICIPAL SEWAGE TREATMENT PLANT
D.6.1 Process Description
Plant C156D is a combined municipal industrial sewage treatment
plant (SIC 4952). Figure D.6-1 is a diagram of the unit opera-
tions of the treatment plant. The influent passes through a bar
screen, has alum added to it for coagulation, passes through grit
removal, polymer addition for flocculation, primary settling,
flotation, disinfection with chlorine, and final discharge. The
total plant effluent flow rate is 30 MGD. About 25% of the
settleable material is anaerobically digested and the remainder
is held, conditioned, centrifuged to remove water, and incinerated.
ci,
INFLUENT
SCREENING
CC
AD
_ GRIT
AGULANT
DITION
REMOVAL
FLOC
ADD
t
:CULANT
ITION
PRIMARY
SETTLING
1
FLOTATION
I
••»
J
\_
EFFLUENT
DISPOSAL
= 25%
LAND
DISPOSAL
Figure D.6-1
= 75%
-J-^P- ULmniiuuni »wn I • I JIVbJWLnm 1UH T~~~^^
IEMICAL ' ^ ^
CHEMICAL
CONDITIONING
Wastewater treatment diagram for Plant C156D
municipal sewage treatment.
D.6-1
-------
D.6.2 Analysis Results, Plant C156D
The following tables in this section present the data collected
for Plant C156D.
TABLE D.6-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C156D
Temper- Dissolved
Flow ature, oxygen,
m3/d (MGD) pH °C (.°F) mg/L
Comments
99,925 6.4 22.9
(26.4) (73.2)
2.7 Prechlorination effluent sampled by SWCB.
Salinity =0.6 ppt; plant operating
normally (primary plant); effluent
grayish color
Instantaneous measurement.
TABLE D.6-2
WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C156D
(mg/L, except as noted)
Species
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (jjg/L)
Total Kjeldahl nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Concentration
101
43
<0.020
0.125
31.3
<0.20
<0.20
24.7
3
223
116
<25
0.140
1.06
0.196
Detection
limit
6
1.0
0.020
0.010
0.1
0.20
0.20
NRD
0.25
0.5
1.0
25
0.007
NR
NR
Samples exceeded required holding times.
3No detection limit reported.
D.6-2
-------
TABLE D.6-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C156D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organ!cs detected0
10.5 Methylene chloride <10d
15.3 1,2-trans-Dichloroethylene <10^
14.3 1,1-Dichloroethane ' <10
15.1 Chloroform 21
16.1 Trichlorotrifluoroethane 3
17.1 1,1,1-Trichloroethane 16,
17.6 Carbon tetrachloride <10d
20.6 Trichloroethylene
-------
TABLE D.6-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
C156D CORRECTED FOR BACKGROUND3'
o
•
C^
i
]
Peak
No.
25
2
3
4
5
6, 7
8
9-12
13
15,16
17-19
Detention
time,
min
21.10
6.52
7.82
8.85
9.20
11.62
11.70
12.97
13.18
14.32
14.93
i
Compound 1
Internal standard0
Anthracene-d10
d e
Recovery standard '
Phenol-d5
Organics detectedf 'g'h
Benzeneme thanol
Phenol, methyl (isomer)
Phenol, 2-chloro-6-
methyl-
1 , 2-Ethanediol , dibenzo-
ate and/or benzole
acid and/or benzoic
ac id , anhydride
Unknown (73,50,101)
Benzeneacetic acid
Unknown (73,115,129)
Benzenepropanoic acid
plus impurity
Unknown (73,129,60)
Peak area,
total counts
213,306
76,268
41,522
152,730
14,387
166,177
33,442
145,357
7,618
64,738
74,644
Amount Amount
detected, spiked, Recovery,
|jg/L M9/L percent
100
53.7 201 26.7
19.5
71.6
6.7
77.9
15.7
68.1
3.6
30.3
35.0
(continued)
-------
TABLE D.6-4 (continued)
D
*
o>
en
Retention
Peak time, Peak area,
No. min Compound total counts
20
21
22,23
24
26
27
16.47
17.08
18.08
20.78
21.95
23.37
IH-Indole, 2,3-dihydro-4-
methyl plus 10%
unknown
[l,l'-Biphenyl]-2-ol
Unknown (73,129,157,200)
Unknown (129,73,185,228)
lH-Purine-2 , 6-dione ,
3,7-dihydro-l,3,7-
trimethyl
Unknown (73,129,256,213)
Total concentration
5,219
5,411
172,989
26,499
10,381
10,584
Amount Amount
detected, spiked, Recovery,
(jg/L |jg/L percent
2.4
2.5
81.1
12.4
4.9
5.0
437
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 2.5 |jg/L,
relative to anthracene-d10 internal standard.
Anthracene-dxo internal standard added immediately prior to analysis; '50 mg/L injec-
ted equivalent to 100 |jg/L in original sample.
i)euterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-d^,
internal standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown .compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.6-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT C156D CORRECTED FOR BACKGROUND '
o
•
^^
I
RRI
202.34
_j
-
298.83
398.17
498.51
_
-
-
-
-.
-
Peak
No.
26
6
19
31
32
33
1
2
3
4
5
7
Retention
time,
min
21.10
7.65
14.77
25.22
29.03
33.07
4.60
5.90
6.53
6.72
7.13
7.82
i
Compound
£
Internal standard
Anthracene-d10
Recovery standard '
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-di2
Perylene-d12
Organics detected '"'
Propane, l,l-[methylene-
bis(oxy)]bis[2-methyl]
Unknown (105,106,77,120)
1,3,5-Triazine, 2,4,6-
triphenyl and/ or ben-
zonitrile
Benzene, trimethyl (isomer)
and/ or benzene, ethyl
methyl (isomer)
Benzene, dichloro (isomer)
Benzenemethanol
Peak area,
total counts
162,799
103,707
89,014
58,081
25,247
17,731
7,980
15,548
9,087
8,859
9,435
40,333
Amount Amount
detected, spiked,
pg/L fjg/L
100
49.7, 43.2 118.1
31.2, 31.6 101.6
30.2, 35.1 99.3
23.7, 26.8 85.8
24.1, 27.7 90.6
4.9
9.6
5.6
5.4
5.8
24.8
Recovery,
percent
-
42.1, 36.6
30.7, 31.1
30.4, 35.3
27.6, 31.2
26.6, 30.6
(continued)
-------
TABLE,D.6-5 (continued)
RRI
cr>
i
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts ug/L |jg/L percent
Organics detected '"' (continued)
10 9.63 Benzeneethanol and/or bi-
cyclo[2.2.1]heptan-2-ol,
1,3,3-trimethyl and/or
bicyclo[3.3.1]nonan-3-ol
exo 14,556 8.9
12 10.30 Unknown (59,81,95,123,152) 15,118 9.3
13 10.75 Bicyclo[2.2.1]heptan-2-ol,
1,7,7-trimethyl-(isomer)
and/or bicyclo[2.2.1]
heptane-7-methanol, 2-
hydroxy-1,7-dimethyl 12,561 7.7
- 14 10.92 Cyclohexanol, methyl-
(methylethyl) (isomer)
and/or bicyclo[4.1.0]
heptane, 3,7,7-trimethyl,
[lS-(la,3p,6or)] 6,012 3.7
15 11.07 Bicyclo[4.4.1]undeca-l,3,
5,7,9-pentaen-ll-one
and/or naphthalene and/
or azulene 9,100 5.6
16 11.30 3-Cyclohexene-l-methanol,
cr,.a,4-trimethyl-,(S) 54,496 33.5
18 13.52 Unknown (71,108,109,97,
59,137) 5,148 3.2
125.93 21 16.43 Unsaturated hydrocarbon
(97,69,83,70) 4,590 2.8
136.47 23 17.08 [l,l-Biphenyl]-2-ol 4,671 2.9
(continued)
-------
TABLE D.6-5 (continued)
CD
RRI
157.54
222.25
238.17
278.92
549.21
Peak
No.
24
27
28
30
34
Retention
time ,
min
18.38
21.95
22.63
24.37
35.32
Compound
Organics detected '"' (continued)
Unknown phthalate (149,
177,150)
!H-Purine-2,6-dione, 3,7-
dihydro-l,3,7-trimethyl
Cl-containing unknown
(218,183,140,152,220)
Sulfur
Silicon-containing unknown
(207,215,281,233)
Total concentration
Peak area,
total counts
10,614
10,507
5,811
125,014
5,162
230
Amount Amount
detected, spiked, Recovery,
Mg/L pg/L percent
6.5
6.5
3.6
76.8
3.2
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 2.8 pg/L,
relative to the anthracene-d10 internal standard.
CAnthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected
equivalent to 100 (jg/L in original sample.
T>euterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-dio,
internal standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-'Retention time less than biphenyl.
-------
TABLE D.6-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C156D
i
<£>
Concentration in each
fraction
(mg/L)
Extractable organics
.
Analysis
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeabled
% Extractable f
% Chromatographable
% GRAV Chromatographable9,
% Extractables identified
Protocol identification efficiency , %
Purgeable
organics Acid
2.10
0.34
1.00,
<0.13a 0.44°
Base/
neutral
2.00
0.31
0.45
0.23
Total
43
4.10
0.65
1.45
0.80
<0.3
12.9
4.9
15.9
1.6
<1.9
From Table D.6-3.
'From Table D.6-4.
'From Table D.6-5.
of carbon purgeable =
_ Total mass of purgeables
TOC
x 100
e% of carbon extractable = TC°TQCGRAV x 100
f% of carbon Chromatographable = TCOTQCTCG x 100
g% of grav Chromatographable = £§— x 100
IjKAV
\ of extractables identified = Total mass of acid GC/MS + B/N GC/MS x
l.\}\*
Protocol organics identification efficiency summation of Equations d and h.
-------
TABLE D.6-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C156D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3.0
2,010
445
19.2
<1.0
32,000
<2.0
10.8
1,150
24.9
5,280
13.9
16,500
13,100
288
<10.0
145,000
509
<70.0
40.7
180
<25.0
<5.0
<5.0
44.0
not reported.
D.6-10
-------
TABLE D.6-8.
0
o>
i
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C156D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
a
t , min
1.97
2.49
2.76
2.95
3.69
3.91
4.39
4.88
6.08
6.76
7.50
7.77
8.83
9.40
Log tr
0.2945
0.3962
0.4409
0.4698
0.5670
0.5922
0.6425
0.6884
0.7839
0.8299
0.8751
0.8904
0.9460
0.9731
Calcd.
log P
0.39
0.87
1.09
1.22
1.69
1.81
2.04
2.26
2.72
2.94
3.15
3.22
3.49
3.62
Peak
height,
cm
2.0
1.7
1.8
3.0
>15
>15
9.8
9.1
6.1
10.4
4.8
4.2
4.5
9.6
Adjusted
peak
height,
cm
180
150
160
270
>1,400
> 1,400
890
830
560
950
440
380
410
870
Estimated
, concentration Positive
FSD,
%
l,200d
1,000
1,100
1,800
>9,100
>9,100
6,000
5,500
3,700
6,300
2,900
2,600
2,700
5,800
index ,
ppb
49
41
44
73
>360
>3146
233C
220
150
90C
120
100
1056
806
or c
negative
_
-
-
-
-
_
-
-
-
-
-
-
_
+
(continued)
-------
TABLE D.6-8 (continued)
i
H
NJ
Peak
number
15
16
17
18
19
20
21
22
23
t ,a min
10.47
12.62
13.08
16.25
17.88
19.00
19.75
25.88
44.08
Log tf
1.0199
1.1011
1.1166
1.2109
1.2524
1.2788
1.2956
1.4130
1.6442
Calcd.
log P
3.84
4.23
4.30
4.75
4.95
5.07
5.15
5.71
6.81
Peak
height,
cm
1.2
2.5
2.5
0.3
0.5
0.3
0.3
0.7
»15
Adjusted
peak
height,
cm
110
230
230
27
46
27
27
64
»1,400
K
FSD,
%
730
1,500
1,500
180
300
180
180
430
»9,100
Estimated
concentration Positive
index, or
ppb negative
29 +
61 +
61 +
7 +
12 +
7 +
7 +
17 +
»360 +
Retention time.
3
Percent full-scale deflection.
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
a
"Corrected for background interference.
-------
TABLE D.6-9.
CORRELATION3 OF BIOACCUMULATION POTENTIALb AND
GC/MS ANALYSIS TEST RESULTS: PLANT C156D
Organic compounds identified by
GC/MS analysis
Actual sample
Molecular Literature log P values
formula log P value [1] by HPLC method
Dichlorobenzene
(Pentachlorophenol)
(2 , 4-Dichlorotoluene )
1 , 2-trans-Dichloroethylene
Trichlorotrifluoroethane
Benzenemethanol
2-chloro-6-methyl-phenol
1,2-Ethanediol, dibenzoate
Benzoic acid, anhydride
Benzeneacetic acid
Benzenepropanoic acid
3.59C
C6H4C12 3.62d'6
C6HC150 3.81
C7H6C12 4.24
C2H2C12 —
C2C13F3
C7H80
C7H7C10
C16H14°4
C14H1003
C8H802
C9H1002
3.626
3.84
4.23
4.30
4.75
4.95
5.07
5.15
5.71
6.81
IH-Indole, 2,3-dihydro-4-methyl
[l,l-Biphenyl]-2-ol
lH-Purine-2,6-dione, 3,7-dihy-
dro-l,3,7-trimethyl-
1,1'- [methylenebis(oxy) ]bis [2-
methyl] -propane
2,4, 6-Triphenyl-l , 3 , 5- triazine
Ethyl methyl benzene
Benzeneethanol
C12H100
C8H10N402
C9H2002
C2 jHi 5N3
C9H12
(continued)
D.6-13
-------
TABLE D.6-9 (continued)
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
Bicyclo[2.2.l]heptan-2-ol,1,3,
3-trimethyl C10H180
Bicyclo[3.3.1]nonan-3-ol, exo C9H160
Bicyclo[2.2.1]heptan-2-ol, 1,7,
7-trimethyl C10H180
Bicyclo[2.2.1]heptane-7-
methanol,2-hydroxy-l,7-
dimethyl C10H1802
Methyl-(methylethyl)-cyclo-
hexanol CioH20°
Bicyclo[4.1.0]heptane, 3,7,
7-trimethyl-, [lS-(lot,3B,6a)] C10H18
Bicyclo[4.4.1]undeca-l,3,5,7,
9-pentaen-ll-one CiiHgO ~
3-Cyclohexene-l-methanol,.
or,a,4-trimethyl-, (S) C10H180
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
>3.5 and >25% FSD).
cOther values reported are 3.01, 3.30, 3.37, 3.40, 3.45.
dOther values reported are 3.38, 3.39, 3.55, 3.60.
ePotential correlation.
Tentative identification based solely on log P values and similar compounds
found in sample.
%o literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing Molecu-
lar Carbon Content and Increasing Log P, 1980.
D.6-14
-------
TABLE D.6-10. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT C156D
Time
24-Hour
48-Hour
7 2 -Hour
96-Hour
LC50 value,
% of neat effluent
25.5
25.0
24.7
24.1
95%
Confidence limits
21.3 - 31.2
20.8-30.6
20.2 - 30.5
19.7 - 29.9
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
D.6.3 Data Interpretation
D.6.3.1 Evaluation of Protocol Performance—
Effluent from Plant C156D was moderately to highly toxic to fat-
head minnows (LC50 = 24.1%; 96 hr). The inorganic and ionic
analyses indicated the presence of free chlorine from the waste-
water treatment, and chromium, copper and zinc at the 1,150 pg/L,
25 pg/L and 44 pg/L levels. Even though the TOC level (43 mg/L)
was below the decision point of 50 mg/L, GC/MS analysis of the
organic content was initiated. These analyses indicated the
presence of many chlorinated organic species which could con-
tribute to the observed toxicity.
In this case the protocol succeeded in identifying many potential
sources of the toxicity.
D.6.3.2 Protocol Changes Suggested for Plant C156D—
No suggested changes.
D.6.3.3 Potential Sources of Chemical Toxicity—
Primary sources of effluent water toxicity are presumed to be
free chlorine from the wastewater treatment plant, chromium at a
level of 1,150 pg/L, copper at a level of 25 pg/L and potentially
zinc at a level of 44 pg/L. Although the TOC value for the
D.6-15
-------
wastewater was low (43 mg/L) the species identified were largely
chlorinated aliphatics and aromatics and therefore could contri-
bute to the overall toxicity which was observed. In addition,
the phenol level of 125 pg/L could be significant as a toxicant.
D.6-16
-------
D.7 PLANT C157D: NYLON RESINS AND FIBERS MANUFACTURE
D.7.1 Process Description
Plant C157D is a chemical manufacturer producing several types of
nylon 6 resins and fibers (SIC 2821).
Figure D.7-1 is a water flow and wastewater treatment diagram for
the plant. Wastewater generated from the process goes partially
to a recovery unit (9,900 GPD) and is removed by a vendor, and
the remainder goes to chemical ponds where it is held for spray
irrigation disposal. Sanitary wastewater is sent to a stabili-
zation pond, then is diluted with non-contact cooling water,
which is not treated. Hydraulic retention time through the treat-
ment plant is estimated to be 0.75 to 1 hour. The total plant
effluent flow rate is 20.2 MGD.
D.7.2. Analysis Results, Plant C157D
The following tables in this section present the data collected
for Plant C157D.
D.7-1
-------
13.716.500
RIVtR WATfR
NON-CONTACT^
COOLING
RAW
4.300,500
I
ts>
NON- CONTACT
~~ COOIING
3.Z20.100
10.5M.400
3.220,100
/ I
4,870,700
ALL FLOWS ARE IN GPD.
js.opo _^
SPRAY IRRIGATION
37.500
RAIN
5.633,700
8.099.800
81.000
20.122,600
6.250
EACH
001
8.093.550
002
SAMPLING POINTS
I
O—»* DISCHARGE
20,116.350
» DISCHARGE
Figure D.7-1. Water flow and wastewater treatment diagram for
Plant C157D: nylon resin and fiber production.
-------
TABLE D.7-1.
SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C157D
Flow
m3/d (MGD) pH
Temper- Dissolved
ature, oxygen,
°C (°F) mg/L
Comments
80,620
(21-3)
7.9 24
(75.2)
7.5 Plant operating normally; effluent
slightly yellowish tint.
has
TABLE D.I-2.
WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C157D
(mg/L, except as noted)
Species
BOD 5
Total organic carbon
Total cyanide
Total phenol
-Total suspended solids
Total mercury (pg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Concentration
<6
65
<0.020
<0.010
6.0
<0.20
<0.20
0.581
0.250
0.542
<0.25
17.5
16.9
<2.5
0.078
0.187
0.104
Detection
limit
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
' 0.5
1.0
2.5
0.007
NR
NR
No detection limit reported.
D.7-3
-------
TABLE D.7-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C157D, CORRECTED FOR BACKGROUNDa
RetentionConcentration,
time, min Compound pg/L
Internal Standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected0
10.5 Methylene chloride <10d
12.6 Trichlorofluoromethane
27.5 Toluene <10
32-33 Xylenes 1
Total concentration6 <31
aBackground correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
°Quantitated relative to area of major ion of authentic
standards.
10 |jg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.7-4
-------
TABLE D.7-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
C157D CORRECTED FOR BACKGROUND '
I
in
Peak
No.
4
2
1
Retention
time,
min
21.08
6.50
3.38
Compound
Internal standard
Anthracene -d10
Recovery standard '
Phenol-d5
Organics detected '"'
Unknown (83,69,97)
Amount Amount
Peak area, detected, spiked, Recovery,
total counts M9/L M9/L percent
222,199 100
48,548 56.7 223 25.4
4,280 1.9
(continued)
-------
TABLE D.7-4 (continued)
O
»
•xl
I
er>
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pg/L pg/L percent
Organics detectedf'g/h
(continued)
12.55 Unknown (113,85,55)
possibly 2H-Azepin-
2-one-hexahydro-
29.90 Phthalate
Total concentration1
8,452
4,422
3.8
2.0
7.7
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH
peak identification program in the Hewlett-Packard system, and are greater than
1.9 \ig/L relative to the anthracene-d10 internal standard.
£
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 M9/L in original sample.
T)euterated recovery standard (spike), added to original effluent before
extraction. v
eO_uantitated related to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that
of anthracene-djo. internal standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according
to decreasing intensities. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
•J
I
TABLE D.7-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT D157D CORRECTED FOR BACKGROUND '
Retention
RRI
201.87
_j
-
298.36
397.91
498.02
Peak
No.
5
1
2
6
29.02
8
time,
mm
21
7
14
25
.08
.65
.77
.20
Compound
Internal standard0
Anthracene-djo
Recovery standard '
1 , 2-Dichlorobenzene-d4
Biphenyl-d1()
Pyrene-d10
Chrysene-d12
33
.05
Perylene-d12
Peak
total
160
173
195
136
62
39
area,
counts
,549
,358
,709
,089
,537
,322
Amount
detected,
pg/L
100
78.3
75.1
84.1
65.1
64.4
Amount
spiked,
(jg/L
-
118.
Recovery,
percent
1
101.6
99.
85.
3
8
90.6
-
66
73
84
75
71
.3
.9
.7
.9
.1
(continued)
-------
TABLE D.7-5 (continued)
U
•
-j
oo
RRI
106.32
182.50
Peak
No.
3
4
Retention
time,
min
15.22
19.92
Compound
Organics detectedf 'g/h
Benzene, l,l'-oxybis
Unknown (188,187,160)
Total concentration
Peak area,
total counts
5,697
9,471
Amount
detected,
3.5
5.9
9.4
Amount
spiked, Recovery,
Hg/L percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above at 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 3.5 ug/L
relative to the anthracene-dio internal standard.
f*
Anthracene-dio internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 M9/L in original sample.
Lleuterated recovery standard (spike), added to original effluent.
eQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-d10
internal standard.
^Tentative identification, not confirmed with authentic standards.
^lajor masses of unknown compounds are listed in parentheses according to decreasing
intensities. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
-------
TABLE D.7-6. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C157D
D
-j
Concentration in each
fraction
(mg/L)
Extractable organics
Analysis
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
Q-
% Purgeable
% Extractable6 -
% Chromatographable
% GRAV Chromatographable^,
% Extractables identified
Protocol identification efficiency , %
Purgeable
organics Acid
0.38
0.01
* ND .
<0.03a <0.01D
Base/
neutral
0.75.
NDJ
0.03
<0.01C
Total
65
1.13
0.01
0.03
<0.05
1.8
0.9
<0.2
From Table D.7-3.
'From Table D.7-4.
:From Table D.7-5.
of carbon purgeable = Total mass of^purgeables x 100
A
% of carbon extractable =
TCO + GRAV
TOC
X 100
TCO + TCG
TCO
x 100
x 100
% of carbon Chromatographable =
g% of grav Chromatographable =
*% of extractables identified = Total mass of acid^GC/MS + B/N GC/MS x 10Q
Protocol organics identification efficiency summation of Equations d and h.
DND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.7-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C157D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
<3
480
<80.0
32.2
<1.0
18,100
<2.0
<6.0
54.1
37.0
1,060
<10.0
3,180
3,650
63.2
<10.0
15,000
33.6
<70.0
<40.0
82.6
29.4
<5.0
<5.0
<40.0
Suspended
solids
*Data not reported.
D.7-10
-------
TABLE D.7-8.
O
vj
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C157D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
t , min
1.84
2.10
3.42
3.63
4.00
4.45
5.25
5.99
7.15
7.46
8.17
11.38
13.00
18.88
31.83
Log tr
0.2648
0.3222
0.5340
0.5599
0.6021
0.6484
0.7202
0.7774
0.8543
0.8727
0.9122
1.0561
1.1139
1.2760
1.5028
Calcd.
log P
0.18
0.48
1.58
1.71
1.93
2.17
2.54
2.84
3.24
3.34
3.54
4.29
4.59
5.43
6.61
Peak
height,
cm
1.9
0.7
2.2
5.1
1.0
0.9
0.8
6.5
0.3
0.6
7.6
0.2
0.1
0.1
0.1
Adjusted
peak
height,
cm
160
60
190
440
86
77
69
560
26
52
650
17
9
9
9
Estimated
. concentration
FSD, index,
% ppb
l,100d
400
1,300
2,900
570
520
460
3,700
170
340
4,400
110
57
57
57
44
16
50
656
18e
21
18
ioe
<1
7e
20C
3e
2
2
2
Positive
°*- c
negative
•
-
_
_
-
-
-
-
-
.
+
+ •
+
+
+
i
a
Retention time.
Percent full-scale deflection.
•^
"A positive response is defined as lop P^3.5 with % FSD >25.
See Appendix C for an explanation of FSD in excess of 100.
"Corrected for background interference.
-------
TABLE D.7-s9.
CORRELATION3 OF BIOACCUMULATION POTENTIALb AND
GC/MS ANALYSIS TEST RESULTS: PLANT C157D
Organic compounds identified by Molecular
_ GC/MS analysis formula
Literature
log P value [1]
Actual sample
log P values
by HPLC method
Benzene, 1,1'-oxybis-
3.54
4.29
4.59
5.43
6.61
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate
(log P £3.5 and £25% FSD)
^
"No literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
TABLE D.7-10.
CALCULATED LC5o'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE C157D IN
STATIC, UNAERATED ARTIFICIAL SEAWATER
Hour
24
48
72
96
LC50,
% effluent
>100
>100
>100
77
95% confidence limits,
% effluent
b
~K
•*
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based on
nominal concentrations of test mate-
rial in artificial seawater. pH
and dissolved oxygen remained with-
in acceptable ranges throughout
the test.
aAssay performed by E.G. & G. Bionomics;
Pensacola, Florida.
Confidence limits not calculated due to
LC50 >100%.
GConfidence level not calculated.
D.7-12
-------
D.7.3 Data Interpretation
D.7.3.1 Evaluation of Protocol Performance—
The wastewater effluent from Plant C157D exhibited low toxicity
to mysid shrimp (LC50 = 77%; 96 hr). Based on the inorganic and
anionic analyses, potential sources of the toxicity could be copper
at 37 pg/L and possibly zinc at 40 pg/L. Since the wastewater TOC
of 65 mg/L exceeded the protocol decision point of 50 mg/L, GC/MS
analysis of the organic materials was initiated. Unfortunately
almost all of the organic materials in the wastewater were non-
chromatographable and the percentage of organics identified was
less than 0.2% of the total. The test for bioaccumulative com-
pounds indicated five peaks exceeding a value of log p >3.5. Of
these only one peak at log P = 3.54 was of significant intensity,
and this material was not characterized.
D.7.3.2 Protocol Changes Suggested for Plant C157D—
Due to the low toxicity of plant C157D effluent, no changes are
recommended in the protocol. For other plants where the toxicity
is higher and suspected to be organic in nature, and the organic
material is largely not chromatographable, other means of instru-
mental" analyses should be employed to characterize the high boiling
organic compounds.
D.7.3.3 Potential Sources of Chemical Toxicity—
Candidate sources for the low toxicity of the effluent from Plant
C157D include copper at a concentration of 37 pg/L and perhaps a
contribution from zinc at 40 pg/L. It is also possible that un-
identified organics (higher boiling) that could not be determined
by GC/MS could have accounted for the observed toxicity.
D.7-13
-------
D.8. PLANT C161D: MUNICIPAL SEWAGE TREATMENT PLANT
D.8.1. Process Description
Plant C161D is a combined municipal/industrial sewage treatment
plant (SIC 4952) with primary treatment.
Figure D.8-1 is a wastewater treatment diagram for the plant.
Influent is screened, goes through grit collectors, and enters
sedimentation tanks. The overflow then goes to a chlorine con-
tact tank and is discharged. The sludge from the sedimentation
tank goes to primary digesters, a secondary digester, elutriation
basins, and vacuum filtration before sludge disposal. The total
plant effluent flow rate is 15 MGD.
INFLUENT
BAR
SCREENS
VACUUM
SLUDGE FILTERS
DISPOSAL
ELUTRIATION
BASINS
SEDIMENTATION
TANKS
SAMPLING
POINT
_ r>
DISCHARGE
PRIMARY
DIGESTORS
SECONDARY
DIGESTOR
Figure D.8-1.
Wastewater treatment diagram for Plant C161D:
Municipal sewage treatment.
D.8-1
-------
D.8.2 Analysis Results, Plant C161D
The following tables in this section present the data collected
for Plant C161D.
TABLE D.8-1
SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C161D
Flow
m3/d (MGD)
39,740*
(10.5)
Temper-
ature ,
pH °C (°F)
6.6 22.6
(71.7)
Dissolved
oxygen ,
mg/L Comments
1.1 Chlorine = 1.6 mg/L; Salinity- 0.63 ppt;
effluent was aerated from time it
brought to lab to decrease chlorine
content; total chlorine in 100%
tank at beginning of bioassay was
0.06 mg/L. Primary plant, plant
operating normally; effluent gray
in color and had strong sewage odor.
a
Instantaneous measurement.
D.8-2
-------
TABLE D.8-2. WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C161D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
141
60
<0.020
0.055
69.3
0.31
0.28
20.06
19.0
<0.04
1.5
202
52
<25
4.34
6.21
4.90
6
1.0
0.020
0.010
0.1
0.20
0.20b
NRb
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
aSamples exceeded required holding times.
No detection limit reported.
D.8-3
-------
TABLE D.8-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C161D, CORRECTED FOR BACKGROUND
Retention
time, min
13.9
25.6
10.5
15.3
15.1
16.1
17.1
20.6
25.9
30.1
Concentration ,
Compound
Internal Standards
Bromochl or ome thane
1 , 4-Dichlorobutane
Organics Detected
Methylene chloride
1 , 2-rrans-dichloroethylene
Chloroform
Trichlorotrifluoroethane
1,1, 1-Trichloroethane
Trichloroethylene
Tetrachloroethylene
E thy Ibenz ene
Total concentration6
wg/L
400
400
•
<10d
<10d
28
78d
<10d
<10d
<10d
<10Q
<166
Background correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
GQuantitated relative to area of major ion of
authentic standards.
10 pg/L is quantiation limit of computerized
identification system.
eSummation of concentrations of detected com-
pounds, excluding standards.
D.8-4
-------
TABLE D.8-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
C161D CORRECTED FOR BACKGROUND '
O
•
00
in
Peak
No.
16
1
2
3a
3b
4
Retention
time,
min
21.10
6.53
8.85
11.33
11.60
11.93
Compound
Internal standard
Anthracene-d10
Recovery standard'
Phenol-d5
Organics detectedf'g'
Phenol, 4-methyl-
Unknown (122,105,138,
123)
Unknown (73,60,101,115)
Benzothiazole and/ or
Peak area,
total counts
200,084
60,923
108,738
134,629
31,989
Amount Amount
detected, spiked, Recovery,
Hg/L M9/L percent
100 -
55.35 200.6 27.6
54.4
67.3
16.0
1,2-Benzisothiazole
26,411
13.2
(continued)
-------
TABLE D.8-4 (continued)
00
i
Peak
No.
5,6
7
8
9
10
11
12
13
14
15
17
18
Retention Amount Amount
time. Peak area, detected, spiked, Recovery,
min Compound total counts pg/L pg/L percent
12.80
13.57
14.27
14.85
15.15
16.73
17.08
17.98
20.80
21.00
21.70
21.95
Organics detected '"'
(continued)
Benzeneacetic acid
Benzoic acid, 2-hydroxy-
Benzenepropanoic acid
Unknown (73,129,60,115)
1-Propanone , 1- (4
me thoxy-pheny 1 ) -
Cl-containing Unknown
(192,194,128)
[l,l'-Biphenyl]-2-ol
Unknown (73,129,157,
200)
Unknown (73,129,185,
228)
Unknown (160,162,91,64)
Unknown (185,63,97,129)
lH-Purine-2 , 6-dione ,
3,7-dihydro-1.3,7-
trimethyl-
90,313
10,272
27,258
49,306
3,324
21,771
5,977
61,474
36,906
7,195
5,954
7,850
45.1
5.1
13.6
24.6
1.7
10.9
3.0
3.07
18.4
3.6
3.0
3.9
(continued)
-------
TABLE D.8-4 (continued)
O
*
00
Peak
No.
19
20
21
22
23
24
25
26
27
28
29
30
Retention
time,
min
22.62
23.12
23.43
24.32
25.55
25.82
27.70
27.88
28.00
28.52
29.93
33.13
Compound
Unknown (183,140,165,
142)
Unknown (68,83,97,111)
Hexadecanoic acid
plus unknown
Sulfur, mol. (S8)
Unknown (69,83,97,55)
Unknown (73,129,284,
241)
Silicon-containing
unknown (315,393,107)
Unknown (107,256,214)
Unknown (169,107,170)
Silicon-containing
Unknown (389,187,
467)
Phthalate (149,167,197)
Silicon-containing
Unknown (451,218,373)
Peak area,
total counts
7,622
8,390
88,085
59,831
79,056
41,282
16,260
375,880
8,202
9,179
6,237
11,356
Amount Amount
detected, spiked. Recovery,
pg/L pg/L percent
3.8
4.2
44.0
29.9
39.5
20.6
8.1
188
4.1
4.6
3.1
5.7
(continued)
-------
TABLE D.8-4 (continued)
O
oo
i
05
Retention
Peak time ,
No. min
31 35.30
32 35.83
Compound
Silicon-containing
Unknown (207,215,233,
281)
Silicon-containing
Unknown (207,281,386)
Total concentration1
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pg/L Mg/L percent
8,122 4.1
9,610 4.8 -
679
Background correction based on reagent and/or method blank analysis, Appendix J.
includes all substances that were found at or above a 2% threshold on the BATCH
peak identification program in the Hewlett-Packard system, and are greater than
1.7 pg/L relative to the anthracene-d10 internal standard.'
f*
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 Mg/L in original sample.
"Tteuterated recovery standard (spike), added to original effluent before
extraction.
Quantitated related to area of major ion of authentic standard.
Estimated concentration based on area of total ion relative to that
anthracene-d10, internal standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parenthesis according
to decreasing intensities. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.8-5
ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT C161D CORRECTED FOR BACKGROUND '
O
oo
10
RRI
202.34
299.06
398.17
498.51
_
Peak
No.
35
8
18
47
51
53
1
3
Retention
time,
min
21.10
7.65
14.77
25.23
29.03
33.07
3.60
5.90
Compound
Internal standard
Anthracene-d10
Recovery standard 'e
1 ,2-Dichlorobenzene-d4
Biphenyl-djo
Pyrene-d10
Chrysene-d12
Perylene-di2
Organics detectedf 'g/h
Benzene (dimethyl (isomer)
Benzene ethylmethyl
Peak
total
114
163
157
92
47
45
12
area,
counts
,540
,365
,964
,481
,708
,987
,524
Amount
detected,
pg/L
100
74.5
•75.0
71.0
63.7
87.3
10.
9
Amount
spiked,
118.
101.
99.
85.
90.
1
6
3
8
6
Recovery,
percent
63
73
71
74
96
.1
.8
.5
.2
.4
(isomer) and/or
Benzene, 1,2,3-trimethyl
(isomer)
7,366
6.4
(continued)
-------
TABLE D.8-5 (continued)
RRI
_
-
-
-
-
-
-
'-
-
Peak
No.
4
6
9
10
11
12
13
14
15
Retention
time ,
min Compound
6.72
7.13
9.33
9.63
10.10
10.30
10.75
10.93
11.32
Organics detectedf 'g'h
Benzene trimethyl
(isomer) and/or
Benzene ethylmethyl
(isomer)
Benzene dichloro (isomer)
Saturated hydrocarbon
(MW = 156)
Benzeneethanol + 10%
unknown
Unknown (81,136,121,
107)
Unknown (59,81,95,123
152)
Bicyclo [2.2.1] heptan-2
ol, 1,7, 7 -trimethyl
(isomer)
Cl3-containing
Unknown (95,81,123,
71,180)
Cyclohexene, l-methyl-4-
(1-methylethylidene)
cyclohexene, l-methyl-3-
(1-methylethynyl)-, (0)
3-cyclohexene-l-methanol ,
a, a, 4-trimethyl-, (S)
Peak area,
total counts
7,808
11,170
9,246
17,497
8,766
26,193
12,576
6,388
79,402
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
6.8
9.8
8.1
15.3
7.7
22.9
11.0
5.6
69.3
(continued)
-------
TABLE D.8-5 (continued)
oo
RRI
105.67
121.07
125.93
132.42
151.86
157.54
176.18
185.74
196.27
222.25
231.15
238.64
257.84
Peak
No.
19
21
22
23
25
26
28
31
33
37
38
39
40
Retention
time,
min
15.18
16.13
16.43
16.83
18.03
18.38
19.53
20.12
20.77
21.95
22.33
22.65
23.47
Compound
Organics detected^ 'g'h
(continued)
Hydrocarbon (171,85,57,43,135
198)
Unknown (129,171,111)
Unknown (69,83,97,70)
Unknown (71,85,57,99)
Cl2-containing unknown
(83,97,192,69,70)
Unknown phthalate
(149,177,150,176)
Unknown (97,83,69,70)
Phenol , 4-nonyl
Unknown phthalate
(149,129,73,187,135,228)
lH-Purine-2 , 6-dione ,
3,7-dihydro-l,3,7-
trimethyl
Unknown (97,83,69,55)
Cl-containing unknown
(218,183,140,165,220)
Unknown (73,129,256,213)
Peak area,
total counts
7,494
6,611
15,570
6,034
12,667
20,000
13,212
8,225
9,392
8,001
27,754
12,221
91,339
Amount Amount
detected, spiked, Recovery,
|jg/L pg/L percent
6.5
5.8
13.6
5.3
11.1
.
17.5
11.5
7.2
8.2
7.0
24.2
10.7
79.7
(continued)
-------
TABLE D.8-5 (continued)
Retention
RRI
Peak
No.
time,
mm
Compound
Peak
total
area,
counts
Amount
detected,
gg/L
Amount
spiked,
Mg/L
Recovery,
percent
o
•
00
1
to
266.28
281.97
290.63
307.31
314.62
387.47
420.60
549.44
561.80
567.42
41
43-44
45
48
49
50
52
54
55
56
23.83
24.50
24.87
25.55
25.83
28.62
29.93
35.33
35.88
36.13
Organics detected
(continued)
f,g,h
Unknown (192,193,85,71)
Sulfur, mol. (S8)
Unknown (97,83,68,111)
Unknown (97,69,83,55)
Unknown (284,129,73,241)
Unknown (85,125,199,57,
227,299)
Phthalate (149,167,279)
Unknown (215,233,149,
234)
Cholest-5-en-3-ol (3-B)
Unknown (207,264,97,83)
6,777
745,081
14,338
14,728
32,855
8,246
15,852
149,403
116,349
10,889
5.9
650.5
12.5
12.9
28.7
7.2
13.8
130.4
101.6
9.5
(continued)
-------
TABLE D.8-5 (continued)
RRI
Peak
No.
Retention
time,
min
Compound
Peak
total
area,
counts
Amount
detected,
pg/L
Amount
spiked,
pg/L
Recovery,
percent
57
58
38.40
39.17
Organics detected 'g/
(continued)
Unknown (215,233,207,149)
Unknown (207,231,281,161)
Total Concentration
27,238
16,654
23.8
14.5
1393
O
•
oo
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found a or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 5.3 pg/L
relative to the anthracene-d10 internal standard.
Anthracene-djo internal standard added immediately prior to analysis,- 50 mg/L
injected equivalent to 100 pg/L in original sample.
tleuterated recovery standard (spike), added to original effluent.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-d10
internal standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensities. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
\etention time greater than benzo(g,h,i)perylene.
-------
TABLE D.8-6. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C161D
o
00
H
Analysis
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeable
% Extractable6 f
% Chromatographable
% GRAV Chromatographable9,
% Extractables identified .
Protocol identification efficiency , %
Concentration in each fraction (mg/L)
Extractable organics
Purgeable Base/
organics Acid neutral Total
60
5.00 15.40 20.40
1.18 2.21 3.39
0.78, 1-30 2.08
<0.17a <0.68 1.39 .2.24
<0.3
37.5
9.1
61.4
3.5
<3.8
From Table D.8-3.
Table D.8-4.
From Table D.8-5.
of carbon purgeable =
Total mass of purgeables
TOC
x 100
e% of carbon extractable = TC°GRAV x 100
TQC
% of carbon Chromatographable =
f
% of grav Chromatographable =
TOO
x 100
x 10°
of extractab.les identified = Total mass of acid GC/MS -H B/N GC/MS x 10Q
JLvJvx
Protocol organics identification efficiency summation of Equations d and h.
-------
TABLE D.8-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C161D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
7.24
757
924
60.3
<1.00
20,300
<2.00
16.7
1,640
83.7
8,360
13.2
15,600
9,300
379
<10.0
166,000
676
<70.0
160
129
<25.0
<5.0
<5.0
248
not reported.
D.8-15
-------
TABLE D.8-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C161D EFFLUENT
00
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
t , min
2.12
2.76
3.69
3.91
4.87
6.26
6.74
7.43
8.00
8.80
9.36
10.75
11.91
12.63
13.25
16.75
18.05
19.43
25.75
43.74
Log tr
0.3263
0.4409
0.5670
0.5922
0.6875
0.7966
0.8287
0.8710
0.9031
0.9445
0.9713
1.0314
1.0759
1.1014
1.1222
1.2240
1.2565
1.2885
1.4108
1.6409
Calcd.
log P
0.55
1.09
1.69
1.81
2.26
2.78
2.93
3.14
3.29
3.48
3.61
3.90
4.11
4.23
4.33
4.81
4.97
5.12
5.70
6.79
Peak
height,
cm
1.0
1.0
7.4
14.0
3.2
1.1
3.3
1.1
1.0
6.4
3.7
0.5
0.8
0.6
0.6
0.4
0.4
0.3
0.5
»15.0
Adjusted
peak
height,
cm
91
91
670
1,300
290
100
300
100
91
580
340
46
73
55
55
36
36
27
46
»1,400
Estimated
, concentration Positive
FSD, index, or
% ppb negative
610d
610
4,500
8,500
1,900
670
2 ,000
670
610
3,900
2,200
300
490
360
360
240
240
180
300
»9,100
24
24
180
2046
78
27
<16
27
24
1556
<16 +
12 +
19 +
,15 +
15 +
10 +
10 +
7 +
12 +
»360 +
Retention time.
Percent full-scale deflection
f+
A positive response is defined as lop P^35. with % FSD >25.
See Appendix C for an explanation of FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.8-9. CORRELATION*1 OF BIOACCUMULATION POTENTIAL13 AND
GC/MS ANALYSIS TEST RESULTS: PLANT C161D
Organic compounds identified by
GC/MS analysis
Actual sample
Molecular Literature log P values
formula log P value [1] by HPLC method
Dichlorobenzene C6H4C12
(1,2,4-Trichlorobenzene) C6H3C13
(1,2,3-Trichlorobenzene) CeH3Cl3
(2,4-Dichlorotoluen|)e C7H6C12
(Hexamethylbenzene) Claris
(1,2,4,5-Tetrachlorobenzene) C6H2C14
1,2-trans-dichloroethylene C2H2C12
1,2-Benzisothiazole C7H5NS
Benzeneacetic acid C8H802
Benzene propanoic acid c9Hio°2
1-propanone, l-(4-methoxy-
phenyl)- • CioHi2Oj
[l,l'-biphenyl]-2-ol C12H100
lH-Purine-2,6-dione, 3,7-
dihydro- 1,3,7-trimethyl-
Hexadecanoic acid
Dimethyl benzene C8H10
Ethyl methyl benzene CgH12
1,2,3-Trimethylbenzene C9H12
Trimethylbenzene (isomer) CgH12
Benzenethanol C8H100
Bicyclo [2.2.1] heptan-2-ol,
1,7,7-trimethyl C10H180
Cyclohexene,l-methyl-4-
(1-methyl-ethylidene) C10H16
Cyclohexene, l-methyl-3-
(1-methyl-ethenyl)-, (0) C10H16
3.62
C'd
3.
4.
.93
.11
4.24
4.31
4.82
3.61
3.90
4.11
4.23
4.33
4.81
4.97
5.12
5.70
6.79
(continued)
D.8-17
-------
TABLE D.8-9 (continued)
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
3-Cyclohexene-l-methanol, a,
a, 4-trimethyl-, (s) C10H160
Phenol, 4-nonyl cisH240
Cholest-5-en-3-ol
(3, B) C27H460
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate
(log P ^3.5 and S25% FSD)
C0ther values reported are 3.38, 3.39, 3.55.360.
t>otential correlation.
Tentative identification based solely on log P values and similar
compounds found in sample.
No literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P 1980.
D.8-18
-------
TABLE D.8-10.
STATIC", UN AERATED-ARTIFICIAL— SEAWATER
LC50/95% confidence limits,
Hour % effluent % effluent
24
48
72
96
71
30
22
10
50-100
24-39
16-29
8-13
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based on
nominal concentrations of test mate-
rial in artificial seawater. pH
remained within acceptable range
. throughout the test. Low dissolved
oxygen in the 100% effluent would
appear to be the major cause of
mortality in this concentration.
aAssay performed by E.G. & G Bionomics;
Pensacola, Florida.
TABLE D.8-10. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT C161D
LC50 value,95%
Time % of neat effluent Confidence limits
24-Hour
4 8 -Hour
72 -Hour
9 6 -Hour
85
80
80
76
.0
.7
.7
.1
71
71
50
.2
.2
.9
_b
- 90.1
- 90.1
- 100
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
95% Confidence limits not reported.
D.8-19
-------
P.9 PLANT C164D: COMBINED MUNICIPAL/INDUSTRIAL SEWAGE TREATMENT
PLANT
D.9.1 Process Description
Plant C164D is a sewage treatment plant receiving both municipal
and industrial wastes (SIC 4952).
Figure D.9-1 is a wastewater treatment diagram for the plant.
Influent goes through screening, grit removal, primary clari-
fiers, aeration tanks, final clarifiers, chlorination for dis-
infection, and final discharge. The settleable sludge from the
primary clarifiers is thickened, combined with the settleable
sludge from the final clarifiers, and co-thickened, then
incinerated. Wastewater retention time is 12 to 15 hours. The
total plant effluent flow rate is 9.6 MGD.
INFLUENT
PRIMARY
CLARIFIERS
AERATION TANKS
PRIMARY
SLUDGE
THICKENER
ASH TO
LANDFILL
RETURN SLUDGE
CO-THICKENER
FINAL
CLARIFIERS
SAMPLING
POINT
DISCHARGE
Figure D.9-1.
Wastewater treatment diagram for Plant C164D:
municipal sewage treatment plant.
D.9-1
-------
D.9.2 Analysis Results, Plant C164D
The following tables in this section present the data collected
for Plant C164D.
TABLE D.9-1
SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C164D
Flow
m3/d (MGD)
PH
Temper- Dissolved
ature, oxygen,
°C (°F) mg/L
Comments
30,280 7.3 23.0 3.5 Chlorine =2.82 ppm; secondary plant;
(8) (73.4) sludge handling capabilities in proc-
ess of being upgraded; plant not oper-
ating normally; effluent gray in color
a
Instantaneous measurement.
TABLE D.9-2
WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C164D
(mg/L, except as noted)
Species
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (M9/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Concentration
14.8
29
0.045
<0.010
12.0
<0.20
<0.20
20.2
17.8
0.045
2.0
8.5
15.6
<2.5
1.72
2.23
1.93
Detection
limit
6
1.0
0.020
0.010
0.1
0.20
0.20
NR3
NR
0.04
0.25
0.5
1.0
2.5
0.007
NR
NR
aNo detection limit reported.
D.9-2
-------
TABLE D.9-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C164D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound yjg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
3.3
6.8
10.5
12.6
15.1
27.5
32-33
Acrylonitrile
Dichlor odi fluorome thane
Methylene chloride
Trichloro fluorome thane
Chloroform
Toluene
Xylenes
Total concentration6
165,
<10d
<10d
<10d
<10d
<10.
3
<218
Background correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
cQuantitated relative to area of major ion of
authentic standards.
10 yg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.9-3
-------
TABLE D.9-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT C164D CORRECTED FOR BACKGROUND*'D
D
*
to
i
Retention
RRI
202.34
_f
-
298.36
397.91
497.27
Peak
No.
3
1
2
4
5
6
time.
mm
21
7
14
25
29
33
.10
.65
.77
.20
.02
.02
Compound
^
Internal standard
Anthracene-djo
Recovery standard 'e
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-djo
Chrysene-d12
Perylene-d12
Peak
total
157
164
191
109
58
47
area,
counts
,681
,144
,306
,581
,199
,135
Amount
detected,
pg/L
100
78.2
72.
69.
62.
71.
8
6
7
1
Amount
spiked,
pg/L
-
118.1
101.6
99.3
85.8
90.6
Recovery,
percent
-
66
71
70
73
78
.2
.7
.1
.1
.5
(continued)
-------
TABLE D.9-5 (continued)
vO
i
Retention
Peak time ,
RRI No . min
Compound
Organics detected
None detected
Amount
Peak area, detected,
total counts pg/L
Amount
spiked, Recovery,
pq/L percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 2.0 |jg/L,
relative to the anthracene-d10 internal standard.
c
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected
equivalent to 100 ug/L in original sample.
Deuterated recovery standard (spike), added to original effluent before extraction.
g
Quantitated relative to area of major ion for authentic standard.
Retention time less than biphenyl.
-------
TABLE D.9-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C164D
00
Concentration in each fraction
(mg/L)
Extractable organics
TOC
GRAY
TCG
TCO
Sum of
Analysis
organics detected by GC/MS
Purge able
organics Acid
2.10
0.23
0.26,
<0.22a 0.12D
Base/
neutral
2.50
0.24
0.15
NDJ
Total
29
4.60
0.47
0.41
<0.34
Carbon balance
% Purgeable
<0.7
(continued)
-------
TABLE D.9-6 (continued)
Concentration in each fraction (mg/L)
Extractable organics
Purgeable Base/
organics Acid neutral Total
% Extractable8 . 17.3
% Chromatographable 3.0
% GRAV Chromatographable9, 10.2
% Extractables identified . 0.4
Protocol identification efficiency , % <1.1
aFrom Table D.9-3.
b
From Table D.9-4.
cFrom Table D.9-5.
*% of carbon purgeable = Total mass of^purgeables x 1Q()
'% of carbon extractable = J^ x 100
TCP + GRAV
TOC
% of carbon Chromatographable = X'"'"'T'QC'LVV' x 10°
g% of grav Chromatographable = ^i^U x 100
VjKAV
of extractables identified = Total mass of acidGC/MS + B/N GC/MS x 1Q()
Protocol organics identification efficiency summation of Equations d and h.
indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.9-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C164D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
6.09
120
167
10.4
<1.0
49,500
<2.0
<6.0
135
23.2
959
505
16,500
2,540
81.7
16.1
193,000
86.3
<70.0
114
138
<25
<5.0
<5.0
65.0
aData not reported.
D.9-10
-------
TABLE D.9-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C164D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
t , min
1.89
2.23
3.43
3.63
4.13
4.50
4.88
5.44
6.00
7.41
8.20
10.75
11.20
11.50
14.83
16.25
21.80
35.80
Log tr
0.2765
0.3483
0.5353
0.5599
0.6160
0.6532
0.6884
0.7356
0.7782
0.8698
0.9138
1.0314
1.0492
1.0607
1.1711
1.2109
1.3385
1.5539
Calcd.
log P
0.24
0.61
1.58
1.71
2.00
2.20
2.38
2.62
2.85
3.32
3.55
4.11
4.25
4.31
4.89
5.09
5.76
6.88
Peak
height,
cm
3.9
2.0
13.5,
6.9
5.1
3.7
2.8
8.5
2.4
8.5
0.5
0.5
. 0.5
0.2
0.2
0.1
0.2
Adjusted
peak
height,
cm
340
170
1,200
>1,300
590
440
320
240
730
210
730
43
43
43
17
17
9
17
Estimated
, concentration Positive
FSD , index , or
% ppb negative
2,200d
1,100
7,700
>8,600
4,000
2,900
2,100
1,60.0
4,900
1,400
4,900
290
290
290
120
120
57
120
89
46 -
310
>2856
160
1156
85
64
60e
55
50C
, 11 +
11 +
11 +
5 +
5 +
2 +
5 +
Retention time.
Percent full-scale deflection.
-i
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
»
"Corrected for background interference.
-------
TABLE D.9-9. CORRELATION3 OF BIOACCUMULATION POTENTIALb AND
GC/MS ANALYSIS TEST RESULTS: PLANT C164D
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
(Hexachlorocyclohexene)
l-Pentyn-3-ol, 3-ethyl-
2-Cyclohexen-l-ol
2 - Cy c lohexen- 1 - one
Cyclohexane , dichloro
Cyclohexene, 3-chloro-
Bi-2-cyclohexen-l-yl
C6H4C16 4.12
C7H120 -d
C6H100
C6H80
C6H10C12
C6H9C1
C12H18
3.55
4.11
4.25
4.31
4.89
5.09
5.76
6.88
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
>3.5 and >25% FSD).
CTentative identification based solely on log P values and similar compounds
found in sample
NO literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing Molecu-
lar Carbon Content and Increasing Log P, 1980.
D.9-12
-------
TABLE D.9-10. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT C164D
Time
24-Hour
48-Hour
7 2 -Hour
9 6 -Hour
LC50 value,
% of neat effluent
8.0
8.0
6.9
6.9
95%
Confidence limits
b
~b
_b
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
95% Confidence limits not reported.
D.9.3 Data Interpretation
D.9.3.1 Evaluation of Protocol Performance—
The effluent from plant C164D was found to be highly toxic to fat-
head minnows (LC50 = 6.9%, 96 hr). From the inorganic and ionic
analyses, the potential causes for the toxicity were chromium,
copper and zinc, although the individual concentration levels of
each metal were not extremely high. The free chlorine concentra-
tion was measured to be 2.82 ppm (unaerated municipal effluent)
which could also account for the effluent water toxicity. Although
the TOC level in the effluent was only 29 mg/L (less than the 50 mg/L
decision level), GC/MS analysis was initiated to identify organic
species that could contribute to the toxic nature of the effluent.
The subsequent organic analysis indicated a concentration of
acrylonitrile (165 \ig/L) in the purgeable fraction which exceeded
the concentration of organics in the entire extractable fractions.
This level of acrylonitrile is just at the limit for toxicity to
freshwater and saltwater aquatic life, but since it is a purge-
able compound its residence time in the biotesting system may not
be sufficient to fully explain the observed toxicity.
D.9-13
-------
D.9.3.2 Protocol Changes Suggested for Plant C164D—
No changes in the protocol are suggested for a plant similar to
C164D.
D.9.3.3 Potential Sources of Chemical Toxicity—
The primary suspected cause for toxicity of the effluent water
from plant C164D is the residual chlorine from the wastewater
treatment plant. A secondary cause for the observed toxicity
could be the combined effects of chromium, copper and zinc levels
in the effluent (135 yg/L, 23.2 H9/L and 65 [tg/L respectively).
A tertiary cause for the toxicity could be the 165 pg/L concen-
tration of acrylonitrile.
D.9-14
-------
D.10 PLANT C169D: COMBINED MUNICIPAL/INDUSTRIAL SEWAGE
TREATMENT PLANT
D.10.1 Process Description
Plant C169D is a combined municipal/industrial regional sewage
treatment plant (SIC 4952).
Figure D.10-1 is a wastewater treatment diagram for the plant.
Influent is sent through three mechanically cleaned bar screens,
then three grit removal channels to remove 0.2 mm grit. The
wastewater then enters eight primary settling tanks and the
overflow goes to four covered stage aeration basins (retention
time approximately 1.46 hours), then to eight secondary settling
tanks and final discharge. Sludge and scum from the primary and
secondary settling tanks go to concentrators and thickeners,
decant tanks, three vacuum filters, and a multiple hearth
incinerator. Wastewater retention time is approximately 6 to
8 hours. The total plant effluent flow rate is 49.9 MGD.
INFLUENT . SCREENING GRIT . SISS? . AERATION
CHAMBER CHAMBER ^c BASINS
. k
?
/SLUDGE \
(THICKENERS!
y^
DECANT
TANKS
'
VACUUM - r
FILTERS
SECONDARY
SETTLING
TANKS
J
SCUM
CONCEN-
TRATORS
1
TNflTNFRATfiR
DISCHARGE
SAMPLING
POINT
ASH TO
Figure D.10-1.
Wastewater treatment diagram for Plant
C169D: regional sewage treatment plant.
D.10-1
-------
D.10.2 Analysis Results, Plant C169D
The following tables in this section present the data collected
for Plant C169D.
TABLE D.10-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C169D ,
Flow
m3/d (MGD)
pH
Temper-
ature ,
°C (°F)
Dissolved
oxygen ,
mg/L
Comments
117,335 6.3 30.0
(31.0) (86.0)
5.7 Plant operating normally; effluent dark
brown in color and smelled sulfurous.
a
Instantaneous measurement.
TABLE D.10-2.
WET CHEMICAL, ION CHROMATOGRAPHY
AND NPDES RESULTS FOR PLANT C169D
(mg/L, except as noted)
Species
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (ng/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Color (true color units)
Concentration
7.8
18
<0.020
0.012
33
<0.20
<0.20
38.1
38.7
0.110
0.6
420
267
<25
0.87
1.34
1.16
800
Detection
limit
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
NR
No detection limit reported.
D.10-2
-------
TABLE D.10-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C169D, CORRECTED FOR BACKGROUND3
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
10.5 Methylene chloride
12.6 Trichlorofluoromethane <10
15.1 Chloroform 18
27.5 Toluene 11
A
Total concentration <49
aBackground correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
GQuantitated relative to area of major ion of
authentic standards.
10 (jg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.10-3
-------
TABLE D.10-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
C169D CORRECTED FOR BACKGROUND '
o
i
Peak
No.
17
2
1
3, 4
5
6
7
8
9
10
11
Retention
time,
min
21.08
6.52
3.27
7.82
8.90
11.52
11.93
12.80
13.68
14.75
15.02
Compound
Internal standard
Anthracene-dl 0
Recovery standard '
Phenol-d5
Organics detected '"'
Unknown (89,118)
Unknown (59,57,117,87)
Unknown (81,82,80,133)
Unknown (100,75,131,43,
146)
Unknown (135,172,69)
Unknown (87,59,73,104,
131)
Unknown (57,103,45,91,
150)
Biphenyl-d10 spiking
compound
Unknown (159,85,59,113,
131,172)
Peak area,
total counts
194,128
54,608
5,451
39,880
3,722
9,456
6,668
4,728
5,262
9,823
4,582
Amount Amount
detected, spiked, Recovery,
ug/L ug/L percent
r
100
66.6 223 29.9
2.8
20.5
1.9
4.9
3.4
2.4
2.7
5.1
2.4
(continued)
-------
TABLE D.10-4 (continued)
o
i
en
Peak
No.
12
13
14b
14°
15
16
18
19
20
21
22
23
24
Retention
time,
min
15.18
17.33
18.90
19.0
20.35
20.57
22.60
23.02
23.35
23.72 .
25.20
27.75
28.88
Peak area,
Compound total counts
Benzaldehyde ,hydroxy-
methoxy- (isomer)
Unknown (129,101,175)
Unknown (73,72,45,117)
Unknown (115,189,143,
97)
Unknown (159,187,85,
89,117)
Unknown (159,187,85,
89,117)
Unknown (73,117,72,45)
Unknown (85,188,73,72)
Unknown (129,73,256)
Unknown (73,159,187,233)
Pyrene-djo spiking com-
pound
Unknown (121,92,107,133)
1-Phenanthrenecarboxylic
acid, l,2,3,4,4a,9.10,
10a-octahydro-l ,4a-
dimethyl-7-(l-methyl-
ethyl)-, [lR-(lcr,4aB,
lOaa)]- and/or 1-Phe-
nanthrenecarboxylic
acid, l,2,3,4,4a,9,10,
10a-octahydro-l ,4a-
dimethyl-7-(l-methyl-
ethyl)-, [!S-(la,4aa,
10aB)]-
11,073
4,311
23,743
6,047
23,126
15,556
5,072
3,628
4,001
5,861
5,768
3,763
29,435
Amount Amount
detected, spiked, Recovery,
ug/L pg/L percent
5.7
2.2
12.2
3.1
11.9
8.0
2.6
1.9
2.1
3.0
3.0
1.9
-v
15.2
(continued)
-------
TABLE D.10-4 (continued)
o
i
Peak
No.
25
26
27
28
Retention
time, Peak area,
min Compound total counts
29.02 Chrysene-d12 spiking
compound 4,731
29.30 Unknown (302,259,136,
135) 8,853
29.90 1,2-Benzenedicarboxylic
acid, diisooctyl ester 11,324
33.03 Perylene-d12 spiking
compound 6 , 507
Total concentration1
Amount Amount
detected, spiked. Recovery,
jjg/L M9/L percent
2.4
4.6
5.8
3.4
135
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 1.9 ng/L,
relative to anthracene-d10 internal standard.
CAnthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injec-
ted equivalent to 100 |jg/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-djo,
internal standard.
"Tentative identification, not confirmed with authentic standards.
ilajor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.10-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT C169D CORRECTED FOR BACKGROUND '
O
o
RRI
201.87
298.36
397.91
497 . 52
-
138.74
Peak
No.
10
2
6
12
'14
16
1
3
4
5
7
Retention
time,
min
21.08
7.63
14.77
25.20
29.02
33.03
5.38
8.92
9.80'
14.55
17.22
Compound
Internal standard
Anthracene-dlo
Recovery standard '
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf /g'h
Unsaturated hydrocarbon
(95,110,67,82)
Unknown (61,73,89,117,
86,130)
Sulfur- containing unknown
(148,85,101,133)
Unknown (73,72,45,103)
Benzene, 1-cyclobutene-l-
Peak area,
total counts
154,768
153,876
197,399
105,338
43,248
20.420
12,325
19,400
24,805
22,688
Amount Amount
detected, spiked, Recovery,
Mg/L pg/L percent
100
70.7 118.1 59.9
72.9 101.6 71.8
67.0 93.1 72.0
46.5 78.8 59.0
35.9 85.0 42.2
8.0
12.5
16.0
14.7
yl and/or 1,4-Ethanona-
phthalen-2(lH)-one, 3,4-
dihydro-
16,363
10.6
(continued)
-------
TABLE D.10-5 (continued)
p
H
O
00
RRI
166.
193.
238.
315.
419.
78
84
17
67
85
Peak
No.
8
9
11
13
15
Retention
time,
min
18.95
20.62
22.63
25.87
29.90
Unknown
59)
Unknown
Compound
(73,72,45,117,103,
(150,96,178,165,
177)
Unknown (73,117,72,45)
Unknown (73,117,72,45)
Unknown phthalate
(149,167,150,279)
Total concentration1
Background correction based on reagent and/or method
Includes all substances that were found at or above a
Peak
total
332
6
158
27
55
blank analysis
3% threshold
area,
counts
,468
,136
,533
,876
,160
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
214.8
4.0
102.4
18.0
35.6
437
, Appendix J.
on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 8.0 ug/L,
relative to the anthracene-d10 internal standard.
cAnthracene-d1o internal standard added immediately prior to analysis; 50 mg/L injected
equivalent to 100 pg/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
eQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
tentative identification, not confirmed with authentic standards.
rlajor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
1Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
-------
TABLE D.10-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C169D
o
to
Concentration in each
fraction
(mg/L)
Extractable organics
Analysis
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeable
% Extractable f
% Chromatographable
% GRAV Chromatographable5,
% Extractables identified
Protocol identification efficiency1, %
Purgeable
organics Acid
5.80
0.49
0.25,
<0.05a 0.14
Base/
neutral
3.55
0.70
0.60
0.44C
Total
18
9.35
1.19
0.85
0.63
<0.3
56.7
11.3
71.4
3.2
<3.5
From Table D.10-3.
JFrom Table D.10-4.
:From Table D.10-5.
of carbon purgeable = Total mass of^purgeables
100
% of carbon
% of carbon
5% of grav Chromatographable =
= TCO + GRAV
TOC X 1UU
TOC
X 100
x 100
of extractables identified =
Total maSS °f acid
TOC
x 100
Protocol organics identification efficiency summation of Equations d and h.
-------
TABLE D.10-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C169D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3.0
918
130
56.0
<1.0
28,000
<2.0
<6.0
104
23-. 1
1,000
15.0
16,800
4,590
372
<10.0
331,000
79.0
<70
<40
141
<25
23.6
<5.0
142
not reported.
D.10-10
-------
TABLE D.10-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C169D EFFLUENT
O
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
t , min
1.90
2.18
2.28
3.66
4.11
4.66
5.22
6.06
6.68
8.29
9.81
10.62
11.13
12.24
15.08
17.36
22.00
28.93
34.06
36.44
Log tr
0.2788
0 . 3385
0.3579
0.5635
0.6138
0.6684
0.7177
0.7825
0.8248
0.9186
0.9917
1.0261
1.0465
1.0878
1.1784
1.2395
1 . 3424
1.4613
1.5322
1.5616
Calcd.
log P
0.25
0.56
0.66
1.73
1.99
2.28
2.53
2.87
3.09
3.58
3.96
4.13
4.24
4.45
4.93
5.24
5.78
6.39
6.76
6.92
Peak
height,
cm
8.2
7.6
7.6
>15
14.7
8.5
11.4
4.5
8.9
4.6
0.9
0.8
1.0
0.3
6.4
0.05
0.05
0.05
0.1
Adjusted
peak
height,
cm
700
650
650
»1,300
>1,300
1,300
700
970
380
760
390
77
68
85
26
550
4
4
4
9
FSD,b
4,700d
4,300
4,300
»8,500
>8,500
8,400
4,800
6,500
2,600
5,100
2,600
500
460
570
170
3,600
29
29
29
• 57
Estimated
concentration Positive
index , or
ppb negative
190
170
170
»285C
>340
330
190
1206
100
506 +
100 +
21 +
18 +
23 +
7 +
150 +
1 +
1 +
1 +
2 +
Retention time.
Percent full-scale deflection.
t
"A positive response is defined as log P ^3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
g
Corrected for background interference.
-------
TABLE D.10-9. CORRELATION3 OF BIOACCUMULATION POTENTIALb AND
GC/MS ANALYSIS TEST RESULTS: PLANT C169D
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
( t-Buty Ibenzene ) c
(Butylbenzene)
(Phenanthrene)c
• <•
3.58
3.96
C10H14 4.11 4.13
C10H14 4.26 4.24
C14H10 4.46 4.45
4.93
5.24
5.78
6.39
6.76
6.92
1-Phenanthrenecarboxylic acid,
l,2,3,4,4a,9,10,10a-octahydro-
1 ,4a-dimethyl-7- (1-methyl-
ethyl)-,[lR-(la,4aB,10aa)]-
1-Phenanthrenecarboxylic acid,
l,2,3,4,4a,9,10, lOa-octahydro-
l,4a-dimethyl-7-(l-methyl-
ethyl)-,[lS-(la,4aa,10ap)]-
1,2-Benzenedicarboxylic acid,
diisooctyl ester
Benzene, 1-cyclobuten-l-yl
1 , 4-Ethanonaphthalene-2 (1H) -one ,
3,4-dihydro-
C24H3804
C12H120
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
>3.5 and >25% FSD).
Tentative identification based solely on log P values and similar compounds
in sample.
rto literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing Molecu-
lar Carbon Content and Increasing Log P, 1980.
D.10-12
-------
TABLE D.10-10. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT C169D
Time
24-Hour
48-Hour
72-Hour
96-Hour
LC50 value,
% of neat effluent
74.3
60.8
60.8
59.9
95%
Confidence limits
65.2 - 85.6
53.0 - 71.0
53.0 - 71.0
51.8 - 70.6
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
D.10.3 Data Interpretation
D.10.3.1 Evaluation of Protocol Performance— •
Plant C169D produced an effluent with a moderately toxic level,
LC50=59.1% to fathead minnows at 96 hrs. Bioaccumulation fraction-
ation revealed a large number of peaks with log P £3.5. Some of
these were estimated to be of appreciable concentration i.e., 50,
100, 150 ppb. The TOC value was only 1.8 mg/L; however, due to
the large number of bioaccumulation peaks, GC/MS characteriza-
tion was undertaken. The acid fraction was low in concentration,
138 |jg/L, and 90% of the peaks were unidentifiable. The base/
neutral fraction contained 437 pg/L; however, two unidentified
peaks constituted 70% of the sample, 215 and 102 ug/L respectively.
D.10.3.2 Protocol Changes Suggested for Plant C169D—
It is suggested that in a case where a few components comprise
the majority of the sample, extra effort should be made to iden-
tify the compounds, e.g./ the two major compounds in the base/
neutral fraction.
D.10.3.3 Potential Sources of Chemical Toxicity—
Plant C169D had a higher value of ammonia than most of the other
effluents characterized. This component, in addition to the
large number of unidentified organics, is presumed to be the major
D.10-13
-------
source of toxicity exhibited by this effluent. The zinc concen-
tration of 142 pg/L could have a definite contribution to this
toxicity also.
D.10-14
-------
D.ll PLANT B133S: CHEMICAL MANUFACTURE
D.ll.l Process Description
Plant B133S is a chemical manufacturer producing lubricants,
plasticizers, dibasic acid esters, and related compounds. Prod-
ucts from this site include n-octyl-n-decyl phthalate, dioctyl
phthalate, diisooctyl phthalate, diisodecyl phthalate, ditridecyl
phthalate, triisooctyl trimellitate, dioctyl adipate, diisooctyl
adipate, diisodecyl adipate, ditridecyl adipate, dibutyl maleate,
dibutyl phthalate, texanol benzoate, diethyleneglycol adipate
polyesters, butylene glycol adipate polyesters, and dioctyl
sebacate (SIC 2869).
Figure D.ll-1 is a wastewater treatment diagram for the plant.
After going through a primary organics separation system, the
wastewater is treated with sulfuric acid for pH adjustment, and
nutrients are added prior to entry to the aerated digestion
ponds. After the aeration tanks, all wastewaters are combined,
the activated sludge is separated, and polishing ponds remove
setteable material before final discharge. The total plant
effluent flowrate averages 35,000 GPD.
D.ll-1
-------
PRIMARY StPARATION
S»SUM
UOIIIOWASTf
COMPOSITING
PONDS
soiios
POMSHINT,
«* HOUCTIW
SUSPtNOtO SOIIW
TO NAINAGt DITCH
DURING HtAVY
RAINtAU ONLY
EMIRHINCY SIORAM
Figure D.ll-1.
Wastewater treatment diagram for Plant B133S:
lubricant and plasticizers production.
-------
D.11.2 Analysis Results, Plant B133S
The following tables in this section present the data collected
for Plant B133S.
TABLE D.ll-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B133S
Temper- Dissolved
Flow ature, oxygen,
m3/d (MGD) pH °C (°F) mg/L Comments
No data taken by Maryland EPA.
TABLE D.ll-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B133S
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Turbidity (NTu)
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia Nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Color (true color units)
15.2
6.0
65
<0.020
0.032
29
<0.20
<0.20
2.991
0.109
0.042
0.7
162
46
<25
<0.007
0.58
0.307
200
6
1
1.0
0.020
0.010
0.1
0.20
0.2g
NRD
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
NR
iSample at room temperature prior to analysis.
No detection limit reported.
D.ll-3
-------
TABLE D.ll-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B133S, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
6.5 Methylbromide <10d
10.5 Methylene chloride 21,
12.6 Trichlorofluoromethane <10d
17.1 1,1,1-Trichloroethane
-------
TABLE D.ll-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
B133S CORRECTED FOR BACKGROUND '
tn
Peak
No.
42,
43
1
2
3
4
5
6
7
8
9
Retention
time,
min
21.10
6.52
7.10
7.32
7.72
8.33
8.40
9.27
10.10
10.25
Peak area,
Compound total counts
c
Internal standard
Anthracene-d10
Recovery standard '
Phenol-d5
Organics detectedf '9'h
3 - Furanol , te tr ahydro- 2 ,
2,4,4-tetramethyl-
Unknown (70,128,42)
1-Hexanol, ethyl-
(isomer)
Phenol, me thy 1-
(isomer)
Unknown (75,58,73,56,158)
Unknown (69,84,97,55,
142)
IH-Pyrazole, 4-nitro-
plus 20% unknown
Unknown (101,103,55,43,
85)
149,978
42,794
76,684
12,854
8,645
7,089
18,415
2,961
5,365
4,499
Amount Amount
detected, spiked, Recovery,
|jg/L MQ/L percent
100 -
78.6 223 35.2
51.1
8.6
5.8
4.7
12.3
2.0
3.6
3.0
(continued)
-------
TABLE D.I1-4 (continued)
cr«
Peak
No.
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
Retention
time. Peak area,
min Compound total counts
10.90
11.08
11.52
11.75
12.45
12.58
13.15
13.52
13.68
14.20
14.77
15.13
15.28
16.17
16.33
16.50
16.70
16.95
17.05
17.12
Unknown (113,57,71)
Unknown (113,57)
Unknown (99,58,141)
Unknown (127,83,113,141,
156)
Unknown (72,73,87,57)
Unknown (72,57,99)
Unknown (87,69,73,170)
Benzofuran, 2,3-dihydro-
2-methyl- plus 20%
unknown
1 ,2-Benzenedicarboxylic
acid
Unknown (87,59,83,97)
Biphenyl-d10 spiking
compound
Unknown (101,126,95,67,
70)
Unknown (133,176,162)
Unknown (84,69,71)
Unknown (157,101,85,111)
Unknown (85,109,59,43)
Unknown (83,69,95)
Unknown (133,134,105)
Unknown (118,90)
(C2)3~benzene (isomer)
plus 30% unknown
(MW = 190)
6,361
4,069
4,936
5,315
1,922
22,486
10,896
4,249
16,454
7,282
4,309
2,598
29,932
5,714
50,362
8,834
5,502
9,131
1,511
9,364
Amount Amount
detected, spiked, Recovery,
pq/L pq/L percent
4.2
2.7
3.3
3.5
1.3
15.0
7.3
2.8
11.0
4.9
2.9
1.7
20.0
3.8
33.6
5.9
3.7
6.1
1.0
6.2
(continued)
-------
TABLE D.I1-4 (continued)
Peak
No.
30
31
33
34
35
36
37
38
39
40
41
44
Retention
time,
min
17.38
17.70
18.03
18.25
18.33
18.45
18.50
18.67
19.33
20.47
20.77
21.27
Peak area.
Compound total counts
Unknown (87,71,43,69,41,
127)
Unknown (97,69,71,133,
83)
Unknown (156,57,141,145)
Unknown (72,71,70,83,
171)
Unknown (105,143,87,71)
Unknown (71,72,70,83,
171)
Benzeneethananamine, N-
(2-phenylethyl)-, hy-
drochloride and/ or 3H-
Indazole-3-one, 1,2-
dihydro-
Unknown (71,95,55,43)
Unknown (137,69,109)
2H-Cyclopropa [a] naphtha-
len-2-one, 1, la, 4, 5, 6,
7 , 7a , 7b-octahydro-l , 1 ,
7 , 7a- tetramethyl- ,
(laa,7a,7aa,7ba)- and/
or D-Norandrostan-16-
one, (5a)- plus 10%
unknown
Unknown (73,69,129,133,
228)
Unknown (71,111,143,69,
141)
6,624
1,772
4,256
4,571
7,603
18,971
15,519
1,724
5,009
8,931
3,496
7,129
Amount Amount
detected, spiked. Recovery,
|jg/L pg/L percent
4.4
1.2
2.8
3.0 (
5.1
12.7
10.3
1.1
3.3
6.0
2.3
4.8
(continued)
-------
TABLE D.I1-4 (continued)
i
00
Peak
No.
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
Retention
time,
min
21.45
21.82
22.45
22.57
23.08
23.15
23.37
23.55
24.67
25.13
25.50
25.80
26.87
29.92
33.07
Peak area,
Compound total counts
Unknown (114,70,83,190)
2H-l-Benzopyran-2-one ,
6 ,7-dimethoxy-4-
methyl- plus 10%
unknown
D-Arabinose, dipropyl
mercaptal, D- and/or
ethanone, l-[4-(l-
hydr oxy- 1 -me thy le thy 1 )
phenyl]- plus 40%
unknown
Unknown (133,175,118)
Unknown (127,109,153,237,
265)
Unknown (129,157,71,101)
Unknown (73,129,213,69)
Unknown (181,252,182,
183)
Unknown (195,143,111,
115)
Unknown (136,164,182,
127,167)
Unknown (127,99,157,
144)
Unknown (163,127,164)
Unknown (150,93,135)
Phthalate
Perylene-d12 spiking
compound
4,283
15,701
3,559
9,286
13,414
6,105
4,878
2,645
4,958
4,941
4,300
5,023
4,968
7,945
13,174
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
2.9
10.5
2.4
6.2
8.9
4.1
3.2
1.8
3.3
3-3
2.9
3.3
3.3
5.3
8.8
(continued)
-------
TABLE D.I1-4 (continued)
Retention Amount Amount
Peak time. Peak area, detected, spiked, Recovery,
No. min Compound total counts ug/L pg/L percent
Total concentration1 359
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 1.0 pg/L,
relative to anthracene-d10 internal standard.
Anthracene-dto internal standard added immediately prior to analysis; 50 mg/L injec-
ted equivalent to 100 pg/L in original sample.
l)euterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
"Tentative identification, not confirmed with authentic standards.
Viajor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.ll-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT B133S CORRECTED FOR BACKGROUND '
i
H
O
RRI
201.87
_j
-
298.36
397.91
498.02
_
-
-
-
-
Peak
No.
15
5
11
18
18a
20
1
2
3
4
6
Retention
time,
min
21.08
7.65
14.77
25.20
29.02
33.05
4.13
5.58
6.52
7.10
8.40
I
Compound
Internal standard
Anthracene-d10
Recovery standard '
1 ,2-Dichlorobenzene-d4
Biphenyl-djo
Pyrene-djo
Chrysene-d12
Perylene-d12
Organics detected '"'
Mixture; 2H-Pyran-3(4H)-
one, dihydro-6-methyl
plus 10% unknown (MW =
142)
1,3,6-Trioxocane, 2-
methyl-
Phenol-d5 spiking
compound
Unknown (71,86,59,43)
Unknown (75,58,73,56,101,
158)
Peak area.
total counts
147,702
177,453
220,950
128,215
7,101
52,682
96,981
130,334
5,712
69,472
28,334
Amount
detected.
uq/L
100
85.5
88.8
86.3
8.2
88.4
65.7
88.2
3.9
47.0
19.2
Amount
spiked, Recovery,
pg/L percent
_
118.1 72.4
101.6 87.4
99.3 86.9
8.5 96.5
100 88.4
(continued)
-------
TABLE D.I1-5 (continued)
RRI
-
-
_
126.58
154.94
157.86
248.01
255.50
419.85
Peak
No.
7
8
9
10
12
13
14
16
17
19
Retention
time,
min
9.33
10.27
12.53
13.68
16.47
18.22
18.40
23.05
23.37
29.90
Compound
Organics detected '^'
Unknown (83,69,55,84,98,
142)
Unknown (101,103,55,43,85,
58)
Unknown (72,57,99,130,143)
Unknown (73,115,101,57,45,
159)
Unknown (109,85,169,113,
151,197)
Unknown (71,72,70,83,171)
Unknown (similar to Peak
No. 13)
Unknown (127,109,99,153,
193)
Unknown (73,129,213,256)
Unknown phthalate (149,
167,279)
Peak area,
total counts
9,876
44,761
12,596
9,159
17,696
14,357
6,702
13,848
6,033
11,753
Amount Amount
detected, spiked, Recovery,
Mg/L pg/L percent
6.7
30.3
8.5
6.2
12.0
9.7
45
9.4
41
8.0
(continued)
-------
TABLE D.ll-5 (continued)
i
H
to
Retention
Peak time ,
RRI No. min Compound
Total concentration
Amount
Peak area, detected.
total counts wg/L
323
Amount
spiked, Recovery,
ug/L percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 3.9 ug/L,
relative to the anthracene-d10 internal standard.
CAnthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected
equivalent to 100 pg/L in original sample.
oeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE D.ll-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B133S
i
H
00
Concentration in each fraction
(mg/L)
Extractable organics
Purgeable Base/
Analysis organics Acid neutral
TOC
GRAV 10.1 2.3
TCG 1.5 0.52
TCO i-89*, 1-02
Sum of organics detected by GC/MS <0.06 0.36 0.32
Carbon balance
% Purgeable
% Extractable6 f
% Chromatographable
% GRAV Chromatographable9,
% Extractables identified .
Protocol identification efficiency , %
Total
65
12.4
2.02
2.91
<0.74
23.6
7.6
15.3
1.1
aFrom Table D.ll-3.
bFrom Table D.ll-4.
cFrom Table D.ll-5.
^ of carbon tmracablr - Total mass of purgeables ., 00
/o u J- t-dii/uii pu.L y Licuj j_-F onr-hrm ^vt"rnrt't"nVil r> — • - .....T. ^T:**, . v 1 nn
y^ UJL wcii ULJii CA L-i cio UcUJXt: TvSp XUU
"f TC*O + TfT*
o/ -» ^ /~>ArKVk^tri r^ViT"r\msit"r
-------
TABLE D.ll-7
ICAP METALS ANALYZED BY EPA
REGION V, PLANT B133S, |jg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
<3.0
115
2,480
26.2
<1
22,700
<2.0
<6
20.0
31.7
391
14.5
1,800
1,420
<5.0
18.1
587,000
<15
<70
639
67.3
<25
28.9
<5.0
<40
Suspended
solids
NDa
29
110
2.1
ND
800
ND
ND
ND
25.7
69
1.2
410
10
ND
8.1
45,000
ND
ND
67
2.1
ND
ND.
ND
ND
aND = not detected.
D.11-14
-------
TABLE D.ll-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B133S EFFLUENT
I
H
in
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
t ,a min
1.30
1.80
2.03
2.39
3.58
4.52
4.94
5.94
6.44
7.56
8.02
10.72
11.5
14.19
15.19
20.75
34.82
Log tr
0.1139
0.2553
0.3075
0.3784
0.5539
0.6551
0.6937
0.7738
0.8089
0.8785
0.9042
1.0302
1.0607
1.1520
1.1816
1.3170
1 . 5418
Calcd.
log P
-0.56
0.18
0.46
0.83
1.76
2.29
2.50
2.92
3.10
3.47
3.61
4.27
4.43
4.91
5.07
5.78
6.97
Peak
height,
cm
0.2
1.5
1.4
1.2
14.2
4.0
2.4
1.0
0.9
0.4
0.6
1.0
0.2
0.15
0.2
0.1
0.1
Adjusted
peak
height,
cm
17
130
120
100
1,200
340
200
85
77
34
51
85
17
13
17
9
9
Estimated
, concentration Positive
FSD, index, or
% ppb negative
iiod
850
800
680
8,100
2,300
1,400
570
510
230
340
570
110
. 85
110
60
60
<16
34
32
27
2656
91
55
<16
21
9
<1 +
216 +
5 +
3 +
5 +
2 +
2 +
Retention time.
'Percent full-scale deflection.
•»
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.ll-9.
CORRELATION3 OF BIOACCUMULATION POTENTIAL13 AND
GC/MS ANALYSIS TEST RESULTS: PLANT B133S
Organic compounds identified by Molecular Literature
GC/MS analysis formula log P value [1]
Actual sample
log P values
by HPLC method
(Butylbenzene)
3-Furanol, tetrahydro-2,2,4,4-
tetra-methyl-
1-Ethyl-hexanol
Benzofuran, 2,3-dihydro-2-
methyl
Triethyl benzene (isomer)
Benzeneethanamine, N-(2-phenyl-
ethyl)-, hydrochloride
3H-Indazole-3-one, 1,2-dihydro-
2H-Cyclopropa[a]naphthalen-2-
one, l,la,4,5,6,7,7a,7b-
octahydro-1,1,7,7a-tetra-
methyl-, (laa,7a,7aot,7ba)-
C10H14
4.26
C8H1602
C8H180
C9H100
C12H18
C16H19N-C1H
C7H6N20
22
3.61
4.27
4.43
4.91
5.07
5.78
6.97
(continued)
D.11-16
-------
TABLE D.I1-9 (continued)
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
D-Norandrostan-16-one, (5a)- C18H280
2H-l-Benzopyran-2-one, 6,7-
dimethoxy-4-methyl- C12H1204
D-Arabinose, dipropyl mercaptal,
D- CuH2404S2
Ethanone, l-[4-(l^hydroxy-l-
methylethyl)phenyl]- C11H1402
IH-Indene, 1-ethyldineoctahy-
dro-7a-methyl-, C12H20
2H-Pyran-3(4H)-one, dihydro-6-
methyl C6H1002
1,3,6-Trioxocane, 2-methyl- C6H1203
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
>3.5 and >25% FSD).
Tentative identification based solely on log P values and similar compounds
found in sample..
w> literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing Molecu-
lar Carbon Content and Increasing Log P, 1980.
D.11-17
-------
TABLE D.11-10. CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHJA) EXPOSED TO EFFLUENT SAMPLE B133S IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
LC50 ,
Hour percent effluent
24
48
72
96
>100
>100
>100
41
95% Confidence limits,
percent effluent
_b
-
-
32-55
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal concen
trations of test material in artificial
seawater. pH and dissolved oxygen re-
mained within acceptable ranges throughout
the test.
aAssay performed by EG&G Bionomic, Pensacola,
Florida.
Confidence limits not calculated due to LC50
D.11.3 Data Interpretation
D. 11. 3.1 Evaluation of Protocol Performance —
The wastewater effluent from plant B133S exhibited a low level of
toxicity to mysid shrimp (LC50 = 41%; 96 hr). No source of
toxicity is evident based on the anionic and inorganic analyses.
Since the TOC exceeded the 50 yg/L protocol decision point (TOC =
65 mg/L), further characterization of the organic content by
GC/MS was initiated. The majority of the chromatographable
content was not identified and is presumed to be oil and grease-
like substances. As a result of the protocol no source for the
low level of toxicity was identified.
D.11.3. 2 Protocol Changes Suggested for Plant B133S —
The use of LC fractionation preliminary to GC/MS could have
improved chances of identifying specific organic compounds in the
organic matter.
D. 11-18
-------
D.ll.3.3 Potential Sources of Chemical Toxicity—
No sources of the low toxicity of the wastewater effluent from
plant B133S were identified.
D.11-19
-------
D.12 PLANT B141S:
MENT PLANT
COMBINED MUNICIPAL/INDUSTRIAL SEWAGE TREAT-
D.12.1 Process Description
Plant B141S is a combined municipal/industrial sewage treatment
plant (SIC 4952). Figure D.12-1 is a wastewater treatment dia-
gram for the plant. Influent goes through screening, primary
sedimentation, activated sludge using purified oxygen for aera-
tion, secondary clarification, and disinfection with chlorine.
Degritted primary sludge is gravity thickened and blended with
secondary sludge which has been thickened by air flotation.
Thickened, blended sludge is chemically conditioned and dewatered
by pressure filtration. Sludge is incinerated and the ash is
disposed of in a landfill.
W1SI1NU. -cnmr Y^ .
INFLUENT jCRCCN-" ^ '
SEWER J
SCREENINGS
TO
LANDFILL
UJ
1
GRIT
TO •< ' V-
.INCINERATOR
--
SOUTHWEST
— @ * SCREENS —
DIVERSION
SEWER
SETTLING TANKS
SLUDGE STORAGE
AND BLENDING
4 4
i i
i
i
i
GRAVITY
SLUDGE
THICKENERS
4
i
i
DEGRITTERS
t
1
PRIMARY
SETTLING TANKS
_f
-
—
/ CHEMICAL \
(CONDITIONERS)
V J
^ x
/~*\
v 1 / SLUDGE \ PRFSSURF
f -ICONDITIONINGJ niTERs » INCINERATORS
I ASH TO LANDFILL
i SLUDGE FLOTATION
THICKENERS
j
i
X ^*S. SAMPLING
OXYGEN /SECONDARY\ CHLORINE TOJLNT
REACTORS 'ICLARIFIERSJ ' CONTACT TANK ° ms^
^*^_^' 001
Figure D.12-1.
Wastewater treatment diagram for Plant
B141S: combined municipal/industrial
sewage treatment plant.
D.12-1
-------
D.12.2 Analysis Results, Plant B141S
The following tables in this section present the data collected
for Plant B141S.
TABLE D.12-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B141S
Temper- Dissolved
Flow ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments
No data taken by Maryland EPA.
TABLE D.12-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B141S
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (ng/L)
Total Kjeldahl nitrogen
Ammonia Nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
>200
131
<0.020
0.051
114
<0.20
<0.20
32.2
19.8
0.356
1.2
105
32
<25
4.4
7.14
0.57
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
No detection limit reported.
D.12-2
-------
TABLE D.12-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B141S, CORRECTED FOR BACKGROUND
Retention
time, min
13.9
25.6
12.6
15.3
15.1
17.1
20.6
21.2
25.9
27.5
28.3
32-33
Concentration ,
Compound
Internal standards
Bromochlorome thane
1 , 4-Dichlorobutane
Organics detected0
Trichlorofluoromethane
1 , 2-trans-Dichloroethylene
Chloroform
1,1, 1-Trichloroethane
Trichloroethylene
Benzene
Tetrachloroethylene
Toluene
Chlorobenzene
Xylenes
Total concentration8
pg/L
400
400
d
<10j
<10d
<10d
-------
TABLE D.12-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
B141S CORRECTED FOR BACKGROUND '
o
»
H
to
Peak
No.
22
3
1
2
4
5
6
7
9
12
13
Retention
time,
min
21.08
6.53
- •
4.62
6.35
8.83
9.20
9.67
11.35
14.20
14.87
16.15
l
Compound t
Internal standard0
Anthracene-d10
Recovery standard '
Phenol-d5
Organics detectedf 'g'h
Unknown (57,87,45,41)
Unknown (90,73,88,43)
Phenol, methyl- (isomer)
Phenol, chloro-methyl-
( isomer)
Benzeneethanol
Unknown (73,60,101,120)
Benzenepropanoic acid
Unknown (129,73,60,115)
Benzoic acid, 2-hydroxy-,
hydrazide and/ or ben-
zoic acid, 3-hydroxy-,
methyl ester
Peak area,
.otal counts
196,795
48,374
8,093
46,967
106,196
14,954
77,256
3,926
12,759
5 , 307
3,458
Amount Amount
detected, spiked, Recovery,
Hg/L pg/L percent
100
60.1 223 27.0
4.1
23.9
54.0
7.6
39.3
2.0
6.5
2.7
1.8
(continued)
-------
TABLE D.12-4 (continued)
tn
Peak
No.
14
15
16
17
18
19
20
21
23
24
25
26
27
28
Retention
time,
min
16.43
16.73
17.08
17.98
18.05
19.53
20.80
20.98
21.58
21.70
21,93
22,33
22.62
23.13
Peak area.
Compound total counts
Organic detected^ 'g'h
Unknown H/C (69,83,97,
70)
Unknown (192,194,128)
[l,l'-Biphenyl]-2-ol
Unknown (73,129,157,200)
Unknown (73,129,157,200)
1-Tetradecanol and/or
eye lote t r adecane
Unknown (129,73,185,228)
Not a hydrocarbon
Cl-containing unknown
(160,224,96,64)
Unknown - not a hydro-
carbon or alcohol
Unknown - not a hydro-
carbon or alcohol
lH-Purine-2 , 6-dione ,
3,7-dihydro-l,3,7-tri-
methyl- (MW=194)
Unknown hydrocarbon or
alcohol (97,64,83,55)
Cl-containing unknown
(218,183,140,165)
Oxacycloheptadecan-2-one
23,477
6,245
5,312
32,842
39,603
11,608
47,401
5,612
3,640
5,441
6,723
5,510
4,874
27,735
Amount Amount
detected, spiked, Recovery,
|jg/L |jg/L percent
11.9
3.2
2.7
16.7
20.1
5.9
24.1
2.8
1.8
2.8
3.4
2.8
2.5
14.1
(continued)
-------
TABLE D.12-4 (continued)
to
I
cr>
Peak
No.
29
30
31
32
33
34
35
36
37
Retention
time, Peak area,
min Compound total counts
Organics detectedf /g'h
23.42 Hexadecanoic acid
24.30 Sulfur, mol. (S8)
24.87 Unknown alcohol or un-
saturated hydrocarbon
25.47 9,12-Octadecadienoic
acid (2,2)- (MW=140)
25.53 Unknown (97,69,83,264)
25.80 Unknown (73,129,185,284)
27.77 Unknown (169,55,69,57,
71)
28.00 Unknown (169,69,98)
28 . 88 1 -Phenanthrenecarboxylic
acid, l,2,3,4,4a,9,10.
10a-octahydro-l ,4a-
dimethyl-7- (1-methyl-
ethyl)-, [!R-(la,4aB,
lOaot)]- and/ or 1-
phenanthrenecarboxylic
acid, l,2,3,4,4a,9,10,
10a-octahydro-l ,4a-
dimethyl-7-(l-methyl-
ethyl)-, [lS-(lct,4aa,
lOap)]-
85,881
13,892
4,408
6,404
33,462
33,537
7,116
7 , 144
4,268
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
43.6
7.1
2.2
3.2
17.0
17.0
3.6
3.6
2.2
(continued)
-------
TABLE p.12-4 (continued)
O
*
H
N)
-J
Peak
No.
38
39
40
Retention
time,
min
29.92
35.30
35.83
Compound
Organics detected
Phthalate
Unknown (silicon-coating
(207,215,233)
Cholest-5-en-3-ol (38)-
plus unknown
Total concentration
Peak area,
total counts
3,579
12,513
11,117
Amount Amount
detected, spiked, Recovery,
Mg/L [jg/L percent
1.8
6.4
5.6
370
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and greater than 1.8 pg/L,
relative to anthracene-d10 internal standard.
n
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injec-
ted equivalent to 100 pg/L in original sample.
i)euterated recovery standard (spike), added to original effluent before extraction.
A
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.12-5
ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT B141S CORRECTED FOR BACKGROUND '
K>
00
RRI
201.87
_j
-
297.89
398.70
497 . 52
_
-
-
-
Peak
No.
22
3
14
31
33b
36
1
2
4
5
Retention
time,
min
21.08
7.63
14.75
25.18
29.05
33.03
6.37
6.70
9.33
9.65
i
Compound
1 ' c
Internal standard
Anthracene-djo
Recovery standard '
1 ,2-Dichlorobenzene-d4
Biphenyl-djo
Pyrened10
Chrysened12
Perylened12
Organics detected '"'
Unknown (90,72,88,74,116)
Benzene, trimethyl-
(isomer)
Hydrocarbon (57,71,85,156)
Benzeneethanol
Peak area,
total counts
119,719
59,749
55,493
36,564
1,728
15,426
107,732
6,412
9,610
56,110
Amount Amount
detected, spiked, Recovery,
uq/L |jg/L percent
500
145.5 118.1 123
133.0 101.6 131
154.5 99.3 156
13.5 8.5 159
187.0 100 187
450
27
40
234
(continued)
-------
TABLE D.12-5 (continued)
RRI
Retention
Peak time,
No. min
Compound
Amount
Peak area, detected,
total counts uq/L
Amount
spiked. Recovery,
pg/L percent
ro
i
10.30
10.75
11.28
-
105.51
125.93
132.25
157.37
176.01
181.04
9
10
11
12
13
15
16
17
18
19
20
11.48
13.23
13.40
13.52
14.22
15.17
16.43
16.82
18.37
19.52
19.83
Organics detected
f,g,h
Bicyclo[2.2.1]heptan-2-
one, 1,7,7-trimethyl-
plus 20% unknown
Bicyclo[2.2.1]heptan-2-ol,
1,7,7-trimethyl-,
(IS-endo)- (isomer)
Cyclohexene, l-methyl-3-
(1-methylethenyl)-,
(O)-plus 10% unknown
Hydrocarbon (71,57,85,170)
Naphthalene, 2-methyl-
Hydrocarbon (71,57,85,184)
Unknown (90,74,72,44,145,
160)
Unknown (85,74,72,160)
Hydrocarbon (85,71,57,198)
Unknown alcohol or unsat-
urated H/C (83,97,69,70,
55)
Hydrocarbon (85,71,57,212)
Unknown phthalate (149,85,
71,177)
Unknown alcohol or unsat-
urated H/C (97,83,69,55)
Hydrocarbon (71,85,57,240)
6,530
5,740
19,830
10,233
3,774
8,826
8,387
10,802
5,984
57,187
4,089
11,077
18,399
4,568
27.2
24.0
82.8
42.7
15.8
36.9
35.0
45.1
25.0
239
17.1
46.3
76.8
19.1
(continued)
-------
TABLE D.12-5 (continued)
o
to
I
H
0
RRI
195.62
205.15
230.91
249.65
256.67
264.87
273.30
276.82
290.63
307.31
313.84
420.35
484.86
548.76
560.67
Peak
No.
21
23
24
25
26
27
28
29
30
32
33
34
35
37
38
Retention
time,
min
20.73
21.22
22.32
23.12
23.42
23.77
24.13
24.28
24.87
25.55
25.80
29.92
32.52
35.30
35.83
Compound
Organics detected '"'
Unknown (139,185,73,228)
Hydrocarbon (71,85,57,254)
Unknown alcohol or unsat-
urated hydrocarbon (83,
97,111)
Unknown (83,97,69,55)
Unknown (73,149,129,256,
213)
Hexadecanoic acid, ethyl
ester plus 10% unknown
Unknown (256,103,257,73)
Sulfur, mol. (S8)
Unknown alcohol or unsat-
urated H/C (97,83,69,57,
111)
Unknown (97,83,69,264)
Unknown (73,284,129,241,
185)
Unknown phthalate (149,
157,279)
Unknown (69,81,207,281)
Unknown (215,233,373,388)
Cholest-5-en-3-ol (3|3)-
plus 20% unknown
Peak area,
total counts
5,681
4,044
14,851
5,163
47,691
2,517
4,473
10,295
7,212
26,925
27,271
6,620
6,037
68,530
51,61.1
Amount Amount
detected, spiked. Recovery,
pq/L |jg/L percent
23.7
16.9
62.0
21.6
199
10.5
18.7
43
30.1
112
114
' 27.7
25.2
286
216
(continued)
-------
TABLE D.12-5 (continued)
Is)
i
Retention
Peak time ,
RRI No . min
566.29 39 36.08
-k 40 38.35
Compound
Organics detected
Silicon-containing unknown
(207,281,264,354)
Unknown silicon containing
(207,281,215,233)
Total concentration
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pg/L pg/L percent
485 2.0
416 1.7
2,694
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system, and are greater than 3.9 (jg/L,
relative to the anthracene-d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected
equivalent to 100 pg/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
k
Retention time greater than benzo(g,h,i)perylene.
-------
TABLE D.12-6 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B141S
H
NJ
Concentration in each fraction
(mg/L)
Extractable organics
Purgeable
Analysis organics Acid
TOC
GRAV 6.72
TCG 1 . 08
TCO 1 . 07.
Sum of organics detected by GC/MS <0.13 0.37
Carbon balance
% Purgeable
% Extractable6 f
% Chromatographable
% GRAV Chromatographable9.
% Extractables identified
Protocol identification efficiency , %
Base/
neutral
23.40
6.74
3.82
2.69C
Total
131
30.12
7.82
4.89
<3.19
<0.1
26.7
9.7
25.9
2.3
<2.4
From Table D.12-3.
'From Table D.12-4.
:From Table D.12-5.
of carbon purgeable =
_ Total mass of purgeables
TOC
x 100
e% of carbon extractable = TC°TQCGRAV x 100
% of carbon Chromatographable = TC°TQCTCG x 100
Q Tff*1
y% of grav Chromatographable = ggTv x 1°°
\ of extractables identified = Total mass of acid GC/MS + B/N GC/MS x 10Q
XwL-
Protocol organics identification efficiency summation of Equations d and h.
-------
TABLE D.12-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B141S, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
7.55
1,110
537
86.3
<1.0
30,800
4.66
9.37
199
371
1,730
<10
14,100
8,340
116
18.3
97,000
95.8
136
<40
94.3
<25
<5.0
<5.0
167
Suspended
solids
3.17
873
133
NDa
ND
5,300
1.46
3.37
106
248
1,025
ND
1,300
610
15
8.3
25,000
80.8
66
ND
14
ND
ND
ND
11
aNot detected.
D.12-13
-------
TABLE D.12-8.
O
•
M
NJ
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B141S EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
t , min Log t
r' ^ r
1.91
2.26
3.37
3.57
4.39
5.10
5.64
6.30
7.16
8.13
8.76
9.32
10.28
10.73
11.80
0.2810
0.3541
0.5276
0.5527
0.6425
0.7076
0.7513
0.7993
0.8549
0.9101
0.9425
0.9694
1.0120
1.0306
1.0719
Peak
Calcd. height,
log P cm
0.32
0.71
1.62
1.75
2.23
2.57
2.80
3.05
3.35
3.64
3.81
3.95
4.17
4.27
4.49
3.4
2.0
>15
>15
>15
3.5
5.2
4.2
2.3
2.6
2.6
1.3
2.7
0.8
Adjusted
peak
height,
cm
290
170
>1,300
>1,300
>1,300
300
440
360
> 1,300
200
220
220
110
230
68
Estimated
, concentration Positive
FSD, index, or
% ppb negative
l,900e
1,100
>8,500
>8,500
>8,500
2,000
3,000
2,400
>8,500
1,300
1,500
1 , 500
740
1,500
460
>7
46
>340
>285C
>340
80
120
95
>3356
<1 +
59 +
59 +
30 +
596
18 +
(continued)
-------
TABLE 12-8 (continued)
Ni
i
M
U1
Peak
number
16
17
18
19
20
21
22
23
24
t , min
13.59
14.33
15.25
19.0
20.86
22.7
24.3
34.37
51.0
Log tf
1.1332
1.1562
1.1833
1.2788
1.3193
1.3560
1.3856
1.5362
1.7076
Calcd.
log P
4.81
4.94
5.08
5.58
5.80
5.99
6.15
6.94
7.84
Peak
height,
cm
1.0
1.4
, 1.3
0.2
1.6
0.3
0.2
12.7
0.2
Adjusted
peak
height.
cm
85
120
110
17
140
26
17
1,100
17
FSD,b
%
570
800
740
110
910
170
110
7,200
110
Estimated
concentration Positive
index , or
ppb negative
23 +
32 +
30 +
5 +
36 +
7 +
5 +
290 +
5 +
Retention time.
Percent full-scale deflection.
A positive response is defined as log P ^3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.12-9.
CORRELATION* OF BIOACCUMULATION POTENTIAL13 AND
GC/MS ANALYSIS TEST RESULTS: PLANT B141S
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
2-Methylnaphthalene C^HIQ 3.86C
(1,8-Dimethylnaphthalene) C12H12 4.26
(1,7-dimethylnaphthalene) C12H12 4.44
3.64
3.81C
3.95
4.17
4.27
4.49
4.81
4.94
5.08
5.58
5.80
5.99
6.15
6.94
7.84
1.2-trans-Dichloroethylene
Benzeneethanol
C8H100
(continued)
D.12-16
-------
TABLE 12-9 (continued)
Actual sample
Organic compounds identified by Molecular Literature log P values
_ GC/MS analysis _ formula log P value [1] by HPLC method
1 , 7 , 7-Trimethyl-bicyclo [2 . 2.1]
heptan-2-one
1,7,7-Trimethyl-, (IS-endo)-
bicyclo[2.2.1]heptan-2-ol
l-methyl-3-(l-methylethyl)-,
(O)-cyclo-hexene
Hexadecanoic acid, ethyl ester
(3p)-cholest-5-en-3-ol
C10H180
cioHie
Ci8H36°
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
>3.5 and >25% FSD).
Tentative identification based solely on log P values and similar compounds
found in sample..
wo literature log P found.
[1] Hausch, C., Computerized Printout of Log P Values by Increasing Molecu-
lar Carbon Content and Increasing Log P, 1980.
D.12-17
-------
TABLE D.12-10. CALCULATED LC50'S FOR MYSID SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B141S IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
LCg o ,
Hour percent effluent
24
48
72
96
17
16
7
<3
95% Confidence limits,
percent effluent
12
11
4
^
- 25
- 23
-b10
Note: Salinity was 25 o/oo and temperature was
22 °C. Values were based on nominal concen-
trations of test material in artificial
seawater. pH and dissolved oxygen re-
mained within acceptable ranges throughout
the tests .
aAssay performed by EG&G Bionomic, Pensacola,
Florida.
Confidence limits not calculated due to LC50
D.12.3 Data Interpretation
D. 12. 3.1 Evaluation of Protocol Performance--
The effluent from Plant B141S was found to be highly toxic to
mysid shrimp (LC50 = <3% at 96 hr). The TOC value for this plant
was the highest for all plants surveyed (131 mg/L). The trace
metal and anionic species analysis indicated a potential toxico-
logical problem with ammonia (19.8 mg/L), chromium (93.3 pg/L),
and copper (123 ng/L). Due to the extremely high TOC, the deci-
sion tree dictated that further characterization of the organic
content was required and GC/MS and bioaccumulative species frac-
tionation and identification was implemented.
The subsequent organic analyses identified about 90 organic com-
pounds in the purgeable, acid and base-neutral fractions. A num-
ber of these compounds were chlorinated and/or aromatic structures
D. 12-18
-------
A total of 15 peaks of potentially bioaccumulative compounds
(log P ^3.5) were observed. A majority of the organic compounds
were not identified by the mass spectral data. About 1/3 of the
total organic material was chromatographable.
For this plant the analytical protocol succeeded only in confirm-
ing the existance of a large number of organic compounds, but was
insufficient to provide an unambigious identification of the
specific source of the observed toxicity.
D.12.3.2 Protocol Changes Suggested for Plant B141S—
Due to a time-cost constraints the LC class fractionatidn step in
the analytical protocol prior to GC/MS analysis was not conducted.
If this step had been included, much more definitive identifica-
tion of the organic compounds may have been possible. Obviously,
with the availability of modern instrumental analytical tech-
niques a much more thorough characterization of the organic frac-
tions is possible. However, the absence of specific toxicological
information relating toxic effects to chemical composition and the
need to maintain an awareness of cost effectiveness must be con-
sidered before additional analyses are recommended.
D.12.3.3 Possible Sources of Chemical Toxicity—
The primary source of toxicity in the effluent of plant 141S is
presumed to be organic chemical species, and especially the
chlorinated organics to which mysid shrimp are particularly
susceptible. The large majority of the organic content in the
effluent can be ascribed to fatty acids and steriod-based com-
pounds that are associated with human waste and public owned
treatment facilities. Other secondary causes for the observed
toxicity could be the relatively high copper content (123 pg/L)
and free dissolved chlorine from the wastewater treatment plant.
D.12-19
-------
).13 PLANT B142S: INDUSTRIAL WASTE TREATMENT AND RECOVERY
).13.1 Process Description
Plant B142S is an industrial waste treatment and recovery plant
(SIC 4963). Waste acid solutions and chemicals are batch treated
lime, FeS04, Na2SO4, and Na2S to precipitate byproducts,
are recovered for sale from settling lagoons.
Figure D.13-1 is a wastewater treatment diagram for the plant.
Batch discharge of the supernatant from the lagoons occurs at the
end of treatment. These batches generate about 20,000 gallons
per day for 10 days per month, with a retention time in the
lagoons of about 3 days. All known input streams for Plant B142S
are inorganic in nature. Therefore, no chromatographable organics
were expected. Treatment procedures and expected reactions are
given below.
Hydrochloric Acid Pickle Liquor
A. Average content - 10% to 20% HCl, 0.5% to 8.0% Fe,
0.1% to 3.0% Ni, 0.1% to 1.0% Cr
B. Treatment - Add lime to pH 8.0 - 8.5; settle and decant. If
supernatant contains nickel, treat with lime or
sodium sulfide to pH 10-10.5; settle and decant.
Readjust pH to 8.0 - 8.5 before discharge.
C. Reactions
1. 2HC1 + Ca(OH)2 -> CaCl2 + 2H20
2. FeCl2 + 2FeCl3 + 4Ca(OH)2 •» Fe(OH)24- + 2Fe(OH)34-
+ 4CaCl2
3. NiCl2 + Ca(OH)2 •* Ni(OH)2 + CaCl2
4. 2CrCl3 + 3Ca(OH)2 -»• 2Cr(OH)34- + 3CaCl2
D.13-1
-------
WASTE
WATER
ACID
TANK
NEUTRALIZATION
TANK
SETTLING
LAGOONS
DISCHARGE
Figure D.13-1.
if SAMPLE COLLECTION POINT
Wastewater flow diagram for Plant B142S:
waste treatment and recovery plant.
Nitric-Hydrochloric Pickle Liquor
A. Average content - 0.2% to 24% HN03 , 0.01% to 5.0% HCl, 0.5% to
8.0% Fe, 0.1% to 3.0% Ni, 0.1% to 1.0% Cr
B. Treatment - Add lime to pH 8.0 - 8.5; settle and decant. If
supernatant contains nickel, treat with lime or
sodium sulfide to pH 10 - 10.5; settle and decant.
Readjust pH to 8.0 - 8.5 before discharge.
C. Reactions
1. 2HNO3 + Ca(OH)2 •* Ca(N03)2 + 2H20
2. Fe++ + 2Fe"MH" + 4Ca(OH)2 •*• Fe(OH)24- 2Fe(OH)3
3.
4Ca(OH)2 •*• Ni(OH)2 + 2Cr(OH)3
Nitric-Hydrofluoric Pickle Liquor
A. Average content - 1% to 20% nitric acid, 0.1% to 5% hydro-
fluoric acid, 0.5% to 8.0% Fe, 0.1% to 3.0%
Ni, 0.1% to 1.0% Cr
D.13-2
-------
B. Treatment - Add lime to pH 8.0 - 8.5; settle and decant. If
supernatant contains nickel treat with lime or
sodium sulfide to pH 10 - 10.5; settle and decant.
Readjust pH to 8.0 - 8.5 before discharge.
C . Reactions
1. 2HN03 + Ca(OH)2 •» Ca(N03)2 + 2H20
2. 2HF + Ca(OH)2 -» CaF24- + 2H2O
3. Fe++ + 2Fe"'"l"f + 4Ca(OH)2 -» Fe(OH)2+ + 2Fe(OH)3 + 4Ca++
4. Ni"" + 2Cr + 4Ca(OH)2 -»• Ni(OH)2^ + 2Cr(OH)3^ + 4Ca+"1"
Sulfuric Acid Pickle Liquor
A. Average content - 15% sulfuric acid, 7% to 20% iron
B. Treatment - Add lime slurry to pH 8.0 - 8.5
C. Reactions
1. H2SO4 + Ca(OH)2 -» CaSO4i + 2H20
2. PeSO4 + Ca(OH)2 •»• Fe(OH)24- +
Sulfuric-Hydrochloric Pickle Liquor
A. Average content - 10% to 15% hydrochloric, 1% to 2% sulfuric,
3% to 5% iron, 4% to 6% copper
B. Treatment - Lime slurry to pH 8.0 - 8.5; settle and decant.
If supernatant contains copper, treat with sodium
sulfide; settle and decant. Readjust pH to
8.0 - 8.5 before discharge.
D.13-3
-------
C. Reactions
1. 2HC1 + Ca(OH)2 •» CaCl2 + 2H2O
2. H2SO4 + Ca(OH)2 •» CaS044. + 2H2O
3. Fe++ + 2Fe+"t"1" + 4Ca(OH)2 •» Fe(OH)24. + 2Fe(OH)3* + 4Ca
4. Cu"*"*" + Ca(OH)2 -> Cu(OH)2 + Ca++
++
5.
)4 + 2Na2S + Ca(OH)2
8NH3t -t- 12H20
CaS + 4Na
D.13.2 Analysis Results, Plant B142S
The following tables in this section present the data collected
for Plant B142S.
TABLE D.13-1. SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B142S
Temper- Dissolved
Flow, ature, oxygen,
ma/d (MGD) pH °C (°F) mg/L
Comments
No data taken by Maryland EPA
TABLE D.I3-2. WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B142S
(mg/L, except as noted)
Species
Total organic carbon
Total cyanide
Total phenol
Total suspended soilds
Total mercury, ng/L
Filtered mercury, pg/L
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Concentration
57
1.28
0.037
24
<0.20
<0.20
19.6
15.4
565
Detection
limit
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
(continued)
D.13-4
-------
TABLE D.13-2 (continued)
Species
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Concentration
2.4
2,140
860
<250
<0.007
0.166
0.131
Detection
limit
0.25
0.5
1.0
250
0.007
NR
NR
aNo detection limit reported.
TABLE D.13-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B142S, CORRECTED FOR BACKGROUND
Retention
time, min
13.9
25.6
12.6
15.1
20.6
21.2
25.9
27.5
32-33
1
Compound
Internal standards
Bromochloromethane
1 , 4-Dichlorobutane
Organics Detected0
Trichl or ofluorome thane
Chloroform
Trichloroethylene
Benzene
Tetrachloroethylene
Toluene
Xylenes
Total concentration6
Concentration ,
ug/L
400
400
-------
TABLE D.13-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
B142S CORRECTED FOR BACKGROUND '
o
•
H
(*>
Peak
No.
27
5b
1
2
3
4
5a
6
7
8
9
Retention
time,
min
21.10
6.65
3.73
4.12
4.17
4.50
4.62
7.48
8.43
9.63
10.00
Compound 1
^
Internal standard
Anthracene-d10
Recovery standard '
Phenol- d5
Organics detected '"'
Unknown (184, 141, 127)
Unknown (87, 44, 72, 70)
Unknown (67, 96, 87, 95)
Cyclohexene, 3-chloro-
Unknown (57, 87, 45,
100)
Cl-containing unknown
(57, 80, .81, 98,
88, 134)
Cyclohexane, dichloro-
(isomer)
Unknown (91, 92, 122)
Phenol, nitro- (isomer)
Peak area,
total counts
207,707
213
11,653
6,390
25,132
7,785
18,069
81,989
64,385
5,665
80,681
Amount Amount
detected, spiked. Recovery,
pg/L pg/L percent
100
0.52 223 0.23
5.6
3.1
12.1
3.8
8.7
39.5
31
2.7
38.8
(continued)
-------
TABLE D.13-4 (continued)
o
•
I-1
u>
Peak
No.
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Retention
time,
min
10.27
10.93
11.03
11.37
11.70
12.02
12.23
12.40
12.83
13.87
14.75
15.83
15.43
16.05
Peak area,
Compound total counts
Organics detected '"'
(continued)
Unknown (83, 81, 56,
95)
Benzene, trichloro-
(isomer) plus 10%
unknown
Unknown (81, 79, 105,
122)
Unknown (possibly
ethanol, l-(2-butoxy-
ethoxy)
Unknown (81, 79, 55)
Unknown (79, 69, 81,
41)
Unknown (81, 79)
Unknown (81, 79, 117)
Unknown (81, 79)
Unknown (81, 79, 173)
Biphenyl-dn) spiking
compound
Unknown (144, 69, 81,
97)
Unknown (69, 98, 81,
41)
Unknown (69, 81, 98,
180)
4,634
3,970
11,449
21,864
23,481
4,855
88,587
52,790
4,107
4,432
7,984
2,937
20,548
8,684
Amount Amount
detected, spiked, Recovery,
ug/L pg/L percent
2.2
1.9
5.5
10.5
11.3
2.3
42.7
25.4
2.0
2.1
3.8
1.4
9-9
4.2
(continued)
-------
TABLE D.13-4 (continued)
CO
I
00
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
uq/L
Amount
spiked,
Recovery,
percent
Organics detected
(continued)
f,g,h
24
25
26
28
17.38
18.60
20.88
22.20
Unknown (139,
69)
Unknown (198,
Unknown (143,
Unknown (171,
143, 115)
143, 65,
121, 105)
142)
170,
9,385
7,259
43,996
19,419
Total concentration1
4
3
21
9
309
.5
.5
.2
.4
Background correction based on reagent and/ or method blank analysis. Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 1.4 ug/L
relative to the anthracene d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
ueuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
^Tentative identification, not confirmed with authentic standards.
masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.13-5 ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT B142S CORRECTED FOR BACKGROUND '
w
i
RRI
201.87
_j
-
298.36
397.39
498.02
_
-
-
-
-
Peak
No.
34
6
24
37
41b
42
2
3
4
5
7
Retention
time,
min
21.08
7.65
14.77
25.20
29.0
33.05
3.58
4.60
6.37
6.53
7.73
Compound 1
^
Internal standard
Anthracene-d10
Recovery standard '
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf /9'h
Benzene, dimethyl- (isomer) and/or
1,3-cyclopentadiene, 5-(l-methyl-
ethylidene)-
Propane, 1,1, '-[methylenebis(oxy)]-
bis[2-methyl]- plus 10% unknown
Benzenamine and/or benzenamine,
hydrochloride
40% Benzonitrile plus 60%
phenol-ds
Unknown alcohol
Peak area,
total counts
181,31^
138,127
201,189
133,773
3,209
59,034
17,723
19,422
52,150
22,999
85,708
Amount Amount
detected, spiked,
pg/L Mg/L
100
57.9 118.1
57.3 101.6
71.6 99.3
6.1 8.5
73.5 100
9.8
10.7
28.8
12.7
47.3
Recovery,
percent
-
49.0
56.4
72.1
71.8
73.5
(continued)
-------
TABLE D.13-5 (continued)
to
I
RRI
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
106.48
124.15
148.95
Peak
No.
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
26
27
28
Retention
time,
min
8.53
9.65
9.82
10.13
10.95
11.07
11.33
11.67
12.18
12.37
12.65
13.25
13.57
13.88
14.37
v 14.47
15.23
16.32
17.85
Peak area,
Compound total counts
Organics detected '"' (continued)
Ethanone, 1-phenyl- and/or
1-hexanone, 1-phenyl- plus 10%
unknown
Benzeneethanol plus 10% unknown
2-Cyclohexen-l-one, 3,5,5-tri-
methyl-
Phosphoric acid, triethyl ester
Benzene, trichloro- (isomer)
Bicyclo[4.4.1]undeca-l,3,5,7,9-
pentaen-11-one and/or naphthalene
and/ or azulene
Ethahol, l-(2-butoxyethoxy)-
Benzene, trichloro- (isomer)
2 , 3-Pyridinedicarbonitrile and/or
quinoline and/or isoquinoline
2-Butanone, 4-phenyl- plus 10%
unknown
Unknown (117, 91, 132, 92)
Naphthalene, methyl- (isomer)
Naphthalene, methyl- (isomer)
3-Buten-2-ol, 4-phenyl-
Unknown (188, 190, 189, 191)
3-Buten-2-one, 4-phenyl-
Benzene, l,l'-oxybis-
Unknown (100, 101)
Unknown (144, 143, 132, 189)
40,559
21,723
38,392
13,565
187,552
12,292
27,829
55,825
12,717
25,636
29,806
16,818
14,152
15,592
40,866
15,931
10,867
13,459
66,272
Amount Amount
detected, spiked, Recovery,
ug/L ug/L percent
22.4
12.0
21.2
7.5
103
6.8
15.4
30.8
7.0
14.1
16.4
9.3
7.8
8.6
22.5
8.8
6.0
7.4
36.6
(continued)
-------
TABLE D.13-5 (continued)
CO
I
RRI
171.15
179.25
276.58
310.71
Peak
No.
31
33
36
38
Retention
time,
min
19.22
19.72
24.27
25.68
Compound
Organics detected '"' (continued)
Phosphoric acid tributyl ester
Unknown (195, 152, 96)
Unknown (233, 91, 234)
Unknown (233, 91, 234)
Total concentration
Peak area,
total counts
11,671
14,414
26,170
11,102
Amount Amount
detected, spiked, Recovery,
Mg/L pg/L percent
6.4
8.0
14.4
6.1
508
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 6.0 ug/L
relative to the anthracene d10 internal standard.
c
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
i)euterated recovery standard (spike), added to original effluent before extraction.
t±
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10,
internal standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
-------
TABLE D.13-6. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B142S
w
i
M
(O
Concentration in each fraction
(mg/L)
Extractable organics
Analysis
TOC
GRAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeabled
% Extractable f
% Chromatographable
% GRAV Chromatographable5,
% Extractables identified
Protocol identification efficiency1, %
Purgeable Base/
organics Acid neutral
1.8 4.12
0.2 0.5
0.66 1.44
<0.107a 0.31D 0.51
Total
57
5.9
0.7
2.10
0.927
<0.2
14.0
4.9
11.9
1.4
<1.6
From Table D.13-3.
of carbon purgeable =
"From Table D.13-4
Total mass of purgeables
"From Table D.13-5.
TOC
x 100
e% of carbon extractable = TC°TQCGRAV x 100
ff TT'O 4- Tff*
% of carbon Chromatographable = x 100
of grav Chromatographable = g^Xy x 100
of extractables identified = Total mass of acid GC/MS + B/N GC/MS x
X vV^
1Protocol organics identification efficiency summartion of Equations d and h.
-------
TABLE D.13-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B142S, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
16.4
<400
891
108
<5
1,310,000
43.6
232
213
119
<400
60.0
22,300
316,000
28.9
2,220
256,000
689
<350
<200
4,750
<125
29.5
<25
<200
Suspended
solids
a'4
NDa
208
40
ND
ND
33.6
202
173
48.2
ND
10
9,300
ND
3.9
820
ND
614
ND
ND
ND
ND
4.5
ND
ND
aNot detected.
D.13-13
-------
TABLE D.13-8. BIOACCUMULATION POTENTIAL TEST RESULTS, PLANT B142 EFFLUENT
GO
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
t , min Log t
1.93
2.26
3.13
3.28
3.56
3.96
4.34
5.41
5.77
6.30
7.28
7.73
8.08
8.66
9.37
10.47
11.56
12.94
14.00
15.38
20.32
0.2856
0.3541
0.4955
0.5159
0.5515
0.5977
0.6375
0.7332
0.7612
0.7993
0.8621
0.8882
0.9074
0.9375
0.9717
1.0199
1.0630
1.1119
1.1461
1.1870
1.3079
Calcd.
log P
0.34
0.71
1.45
1.56
1.75
1.99
2.20
2.70
2.85
3.05
3.38
3.52
3.62
3.78
3.96
4.22
4.44
4.70
4.88
5.10
5.74
Peak
height,
cm
12.2
1.8
>15
>15
>15
>15
>15
8.4
4.7
6.8
3.9
4.6
4.9
2.7
2.7
1.7
0.7
0.6
0.3
0.6
0.1
Adjusted
peak
height,
cm
1,000
150
> 1,300
>1,300
>1,300
>1,300
>1,300
720
400
580
330
390
420
230
230
150
60
51
26
51
9
Estimated
, concentration Positive
FSD, index, or
% ppb negative
6,900d
1,000
>8,500
>8,500
>8,500
>8,500
>8,500
4,800
2,700
3,900
2,200
2,600
2,800
1 , 500
1,500
970
400
340
170
340
57
280
41
>340
>3386
>2856
>338C
>340
190
<1C
160
846
110 +
<16 +
62 +
62 +
376
16 +
14 +
7 +
14 +
2 +
a
Retention time.
Percent full-scale deflection.
m
"A positive response is defined as log P ^3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.13-9.
CORRELATION OF BIOACCUMULATION POTENTIAL
GC/MS ANALYSIS TEST RESULTS: PLANT B142S
AND
Organic compounds identified by
GC/MS analysis
c
(Dipheny lamine )
Naphthalene
(Hexachlorocyclohexane)
Methylnaphthalene
Trichlorobenzene
p
(Butybenzene)
(2,3, 6-Tr imethylnaphthalene )
(Pentachlorobenzene )
3-Chlorocyc lohexene
Dichlorocyclohexane
Molecular
formula
C12HllN
CioH8
C6H6C16
CH.HIO
CcHgCls
C10H14
C13H14
C5HC15
C6H9C1
C6H10C1
Literature
log P value [1]
3'51d e
3.59°'e
3.78
3.86
3.931'6
4.26
4.73
4.88
_g
-
Actual sample
log P values
by HPLC method
3.52
3.626
3.78
A
3.96C
4.22
4.44
4.70
4.88
5.10
5.74
DimethyIbenzene
5-(l-methylethylidene-l,3-
cyclopentadiene
1,1'-[Methylenebis(oxy)]bis[2-
methyl-]propane
Benzenamine
Benzeneamine, hydrochloride
1-Phenyl-ethanone
1-Phenyl-l-hexanone
Benzenethanol
3,5,5-Trimethyl-2-cyclohexene-
1-one
Bicyclo[4.4.1]undeca-l,3,5,7,
9,-pentaen-11-one
l-(2-Butoxyethoxy)-ethanol
2,3-Pyridinedicarbonitrile
4-Phenyl-2-butanone
4-Phenyl-3-buten-2-ol
C8H10
C6H7N
C6H7N-C1H
C8H80
C16H160
C8H100
C9H140
CliH80
C8H18°3
C10H120
C10H120
(continued)
D.13-15
-------
TABLE D.13-9 (continued)
Actual sample
Organic compounds identified by Molecular Literature log P values
GC/MS analysis formula log P value [1] by HPLC method
4-Phenyl-3-buten-2-one
1,1'-Oxybis-benzene
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds which may bioaccumulate (log P
13.5 and 125% FSD).
Tentative identification based solely on log P values and similar
compounds found in sample.
dOther values reported are 3.01, 3.30, 3.37, 3.40, 3.45.
Potential correlation
fOther values reported are 3.99, 4.11, 4.12, 4.15, 4.49.
^No literature log P found.
[1] Hausch, C. Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
TABLE D.13-10. CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B142S IN
STATIC, UNAERATED ARTIFICIAL SEAWATER
Hour
24
48
72
96
LC50 ,
% effluent
36
19
11
4
95% confidence limits,
% effluent
29-45
14-26
8-J5
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based no
nominal concentrations of the ef-
fluent in artificial seawater. pH
and dissolved oxygen remained with-
in acceptable ranges throughout
the tests.
aAssay performed by EG&G Bionomics,
Pensacola, Florida.
Confidence limits not calculated.
D.13-16
-------
D.13.3 Data Interpretation
D.13.3.1 Evaluation of Protocol Performance—
The effluent from Plant B142S was found to be highly toxic to
mysid shrimp (LC50 = 4% at 96 hr) and exhibited a TOC content of
57 mg/L. As a result, the decision tree required further organic
content identification via GC/MS and bioaccumulation fractionation
and characterization. The inorganic species analyses indicated a
potential toxicity problem due to a high cyanide level of
1.28 mg/L and copper concentration of 70.8 pg/L. High levels of
molybdenum and strontium were also found in the effluent.
The subsequent organic analysis indicated the presence of chlo-
rinated aromatic and aliphatic species. The bioaccumulation
analysis resulted in 10 peaks exceeding the log P >3.5 level,
including trichlorobenzene.
D.13.3.2 Protocol Changes Suggested for Plant B142S—
Due to cost-time constraints, the LC class fractionation step in
the protocol prior to GC/MS analysis was not conducted. The
inclusion of this step would have resulted in a more definitive
identification of the organic compounds in the water effluent.
No specific protocol changes are recommended for cases like
Plant B142S where the TOC is near 50 mg/L.
D.13.3.3 Possible Source of Chemical Toxicity—
The primary source of toxicity in the effluent of Plant B142S is
pressumed to be the high concentration of cyanide ion. Secondary
and tertiary causes would include copper and the chlorinated
aromatic and aliphatic organic compounds in the wastewater.
D.13-17
-------
D.14 PLANT B149S: STAINLESS STEEL MANUFACTURE
D.14.1 Process Description
Plant B149S is a manufacturer of stainless steel bars, plates,
sheets, and strips from purchased scrap and ferroalloys. Opera-
tions at the plant include melting, rolling, and finishing
(SIC 3312).
Figure D.14-1 is a wastewater treatment diagram for the plant.
Weak acid rinse water is sent to lagoons for neutralization with
a dolomite lime slurry. Spent pickle liquor is removed by a
vendor. Oily wastes are segregated, skimmed, and removed from the
plant by a vendor. Retention time through the treatment plant is
about four hours. The total effluent flow rate averages 0.7 MGD.
D.14.2 Analysis Results, Plant B149S
The following tables in this section present the data collected
for Plant B149S.
SAMPLING
POINT
DISCHARGE
Figure D.14-1.
Wastewater treatment diagram for Plant
B149S: stainless steel manufacturing.
D.14-1
-------
TABLE D.14-1.
SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B149S
Flow,
m3/d (MGD)
pH
Temper- Dissolved
ature, oxygen,
°C (°F) mg/L
Comments
No data taken by Maryland EPA
TABLE D.14-2.
WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B149S
(mg/L, except as noted)
Species
Detection
Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended soilds
Total mercury, \ig/L
Filtered mercury, H9/L
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total dissolved phosphorus
Hexavalent chromium, pg/L
8
0.025
0.010
106
<0.20
<0.20
1.352
0.664
50.6
43.0
72.0
325.0
260.0
<0.007K
D
<25
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
2.5
0.007
NR
25
No detection limit reported.
3Interference - not quantified.
D.14-2
-------
TABLE D.14-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B149S, CORRECTED FOR BACKGROUND
Retention
time, min
13.9
25.6
6.8
10.5
17.1
27.5
30.1
32-33
Concentration,
Compound Mg/L
Internal standards
Bromochloromethane
1 , 4-Dichlorobutane
Organics Detected0
Dichlorodi f luoromethane
Methylene chloride
1,1, 1-Trichloroethane
Toluene
Ethylbenzene
Xylenes
Total concentration6
400
400
<10d
<10d
120,
<10d
<10d
20
<180
aBackground correction based on average of organic-
free water blanks.
Internal standards added to sample immediately
before purging.
cQuantitated relative to area of major ion authentic
standards.
10 (jg/L is quantitation limit of computerized
identification system.
eSummation of concentrations of detected compounds,
excluding standards.
D.14-3
-------
TABLE D.14-4. ORGANIC COMPOUNDS IN THE ACID FRACTION FROM EFFLUENT
B149S CORRECTED FOR BACKGROUND '
Peak
No.
19
2
1
3
4
5
6
7
8
9
10
Retention
time,
min
21.12
6.53
3.40
7.47
8.45
9.95
10.60
11.03
11.33
12.10
12.17
Compound 1
Internal standard
Anthracene-d10
Recovery standard '
Phenol-d5
Organics detected '"'
Unknown (83, 69, 97)
Unknown (57, 80, 81, 98)
Cyclohexanediol (isomer)
Unknown (143, 69, 85,
113)
Benzaldehyde , methoxy-
( isomer)
Unknown (81, 79, 102,
87)
Unknown (57, 45, 100,
101)
Phenol, methyl-nitro-
( isomer)
Unknown (81, 79, 57,
55)
Peak area,
total counts
135,732
14,456
2,390
4,347
1,908
9,396
1,588
5,139
10.299
1,105
2,998
Amount Amount
detected, spiked, Recovery,
pg/L M9/L percent
100
75.4 223 33.8
1.8
3.2
1.4
6.9
1.2
3.8
7.6
0.8
2.2
(continued)
-------
TABLE D.14-4 (continued)
0
ui
Peak
No.
11
12
13
14
15
16
17
18
20
Retention
time ,
min
-
13.98
15.20
15.42
.16.07
17.10
17.42
17.70
20.68
23.43
Peak area,
Compound total counts
Organics detected
(continued)
Silicon - containing
unknown (341, 342,
429, 147)
Unknown (71, 85, 57,
151, 152)
Unknown (69, 41, 81,
67)
Unknown (180, 195, 134,
152)
l,l'-Biphenyl-ol
(isomer)
Unknown (143, 69, 113,
139)
Phenol, tetrachloro-
( isomer)
Phenol, pentachloro-
Unknown phthalate (149,
150, 205)
1,245
1,380
1,112
3,666
2,524
2,140
4,796
1,866
748
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
0.9
1.0
0.8
2.7
1.9
1.6
3.5
1.4
0.6
(continued)
-------
TABLE D.14-4 (continued)
Retention Amount Amount
Peak time, Peak area, detected, spiked. Recovery,
No. _ min _ Compound _ total counts pg/L _ pg/L _ percent
Organics detectedf 'g'h
(continued) _ _
21 25.20 Pyrene-d10 spiking
compound 1,169 0.9
Total concentration 44.2
O Background correction based on reagent and/or method blank analysis, Appendix J.
H Includes all substances that were found at or above a 1% threshold on the BATCH peak
i ' peak identification program in the Hewlett-Packard system and are greater than
^ 0.4 \ig/L relative to the anthracene dto internal standard.
f*
Anthracene-djo internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
Deuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of
anthracene-d10, internal standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
-------
TABLE D.14-5
ORGANIC COMPOUNDS IN THE BASE/NEUTRAL FRACTION FROM
EFFLUENT B149S CORRECTED FOR BACKGROUND '
i
vj
RRI
201.88
_j
-
298.59
397.66
498.75
_
-
-
-
-
-
-
-
-
-
-
Peak
No.
63
3
39
59
70
—
2
4
6
8
9
12
13
17
18
20
21
Retention
time,
min
21.10
7.65
14.78
25.22
29.03
33.0
6.92
8.17
9.35
9.82
10.05
10.65
10.73
11.18
11.50
11.77
12.13
Compound
Internal standard
Anthracene-d10
Recovery standard '
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-dj2
Perylene-d12
Orqanics detectedf 'g/h
Unknown (hydrocarbon) (57, 71, 85)
Unknown (105, 138, 134)
n-Cn-alkane
Unknown (152, 95, 81, 82)
Cyclohexane, pentyl-
Unknown (119, 71, 85, 70)
Unknown (119, 85, 71, 57, 148)
Unknown (97, 131, 69, 85)
n-C12-alkane
Unknown (57, 71, 98)
Unknown (133, 95, 81, 137)
Peak area,
total counts
153,694
12,646
28,948
10,247
4,184
1,998
7,464
4,176
23,430
5,070
3,964
6,232
7,036
6,492
43,866
17,986
3,910
Amount Amount
detected, spiked,
Mq/L pg/L
100
72.0 118.1
57.4 101.6
62.7 99.3
56.1 83.0
39.2 85.0
48.6
27.2
152.4
33.0
25.8
40.5
45.8
42.2
285.4
117.0
25.4
Recovery,
percent
-
61.0
56.5
63.1
67.6
46.1
(continued)
-------
TABLE D.14-5 (continued)
o
*
H
*»
I
00
RRI
— l
-
-
-
-
-
-
-
-
-
-
-
. -
103.88
105.98
106.78
110.82
111.96
122.46
132.63
139.10
140.39
157.35
Peak
No.
22
23
26
27
28
30
31
32
33
34
36
37
38
40
41
42
44
45
49
53
55
56
59
Retention
time,
min
12.22
12.43
12.73
12.85
12.90
13.27
13.43
13.58
13.75
14.17
14.47
14.57
14.67
15.07
15.20
15.25
15.50
15.57
16.22
16.85
17.25
17.33
18.38
Compound
Organics detected '"' (continued)
Unknown (83, 82, 55, 168)
Unknown (131, 97, 69, 146)
Unknown (85, 131, 146, 57)
Unknown (85, 71, 57, 155, 154)
Unknown (71, 57, 113, 112)
Naphthalene, methyl- (isomer)
n-C13-alkane
Naphthalene, methyl- (isomer)
Unknown (160, 145, 117, 118)
Unknown (83, 82, 55)
Unknown (71, 85, 70, 131)
Unknown (85, 71, 57, 99)
Unknown (193, 71, 105)
Unknown (141, 145, 156, 160)
n-C14-alkane
Naphthalene, dimethyl- (isomer)
Naphthalene, dimethyl- (isomer)
Naphthalene, dimethyl- (isomer)
plus 10% unknown
Unknown (71, 85, 57)
n-C15-alkane .
Naphthalene, trimethyl- (isomer)
plus 10% unknown
Naphthalene, trimethyl- (isomer)
n-C16-alkane
Peak area,
total counts
4,671
4,177
12,722
5,547
18,942
28,840
50,976
18,341
14,382
12,891
6,134
9,575
5,765
6,913
49,608
17,113
18,610
21,198
15,429
25,280
5,161
4,578
17,275
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
30.4
27.2
82.8
36.1
123.2
187.6
331.7
119.3
93.6
83.9
40.0
62.3
37.5
45.0
322.8
111.3
121.1
137.9
100.4
164.5
33.6
29.8
112.4
(continued)
-------
TABLE D.14-5 (continued)
vD
RRI
181.10
182.39
Peak
No.
61
62
Retention
time ,
min Compound
Organics detected '"' (continued)
19.85 n-C17-alkane
19.93 Unknown (71, 85, 57, 183)
Total concentration
Amount Amount
Peak area, detected, spiked, Recovery,
total counts ug/L pg/L percent
6,918 45.0
5,312 34.6
3,357.3
•a
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak
identification program in the Hewlett-Packard system and are greater than 25.4 (Jg/L
relative to the anthracene d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L
injected equivalent to 100 pg/L in original sample.
iJeuterated recovery standard (spike), added to original effluent before extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that anthracene-djQ,
internal standard.
"Tentative identification, not confirmed with authentic standards.
liajor masses of unknown compounds are listed in parentheses according to decreasing
intensity. Possible molecular ions are underlined.
1Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE D.14-6. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B149S
Concentration in each fraction
(mg/L)
Extractable organics
O
•
H
1
H
O
Analysis
TOC
GKAV
TCG
TCO
Sum of organics detected by GC/MS
Carbon balance
% Purgeable
% Extractable f
% Chromatographable
% GRAV Chromatographable9,
% Extractables identified .
Protocol identification efficiency , %
Purgeable Base/
organics Acid neutral
0.82 30.85
0.25 9.85
0.24 12. 9g
0.18a 0.04 3.3
Total
50
31.67
10.10
13.18
3.58
0.36
90
47
31.9
6.7
7.1
From Table D.14-3.
From Table D.14-4.
'From Table D.14-5.
% of carbon purgeable = Total massif purgeables x 10Q
/o
Of
g% of grav Chromatographable =
of extractables identified =
TCP + TCG
TOC
GRAV X 10°
Total
100
°f
TOC
+ B/N GC/MS x 10Q
Protocol organics identification efficiency summartion of Eguations d and h.
-------
TABLE D.14-7. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B149S, |jg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3
1,200
206
112
<1.0
84,000
4.28
331
10,300
684
62,300
42.2
11,600
9,020
2,840
851
171,000
14,100
220
<40
250
70.4
26.7
<5
3,940
aData not reported.
D.14-11
-------
TABLE D.14-8.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B149S EFFLUENT
N)
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
t , min Log t
1.90
2.17
2.38
2.79
3.16
3.28
3.58
4.29
5.00
5.86
6.68
7.31
8.12
8.70
9.25
10.60
11.80
12.75
14.28
14.86
16.30
17.37
18.83
20.45
21.8
24.66
0.2788
0.3365
0.3765
0.4456
0.4997
0.5159
0.5539
0.6325
0.6990
0.7679
0.8248
0.8639
0.9096
6.9395
0.9661
1.0253
1.0719
1.1055
1.1547
1.1720
1.2122
1.2398
1.2749
1.3107
1.3385
1.3920
Calcd.
log P
0.31
0.61
0.82
1.19
1.47
1.56
1.76
2.17
2.52
2.89
3.19
3.39
3.63
3.79
3.93
4.25
4.49
4.67
4.93
5.02
5.23
5.38
5.56
5.75
5.90
6.18
Peak
height,
cm
8.2
5.6
5.2
3.0
7.2
7.3
11.6
8.5
3.3
5.0
7.2
2.6
14.2
>15
2.3
>15
>15
2.6
13.9
>15
3.8
4.0
4.9
>15
8.1
4.3
Adjusted
peak
height,
cm
700
480
440
260
610
620
990
730
280
430
610
220
1,200
>1,300
200
>1,300
>1,300
220
1,200
>1,300
320
340
420
>1,300
690
370
Estimated
, concentration Positive
FSD, index, or
% ppb negative
4,700d
3,200
3,000
1,700
4,100
4,200
6,600
4,800
1,900
2,800
4,100
1,500
8,100
>8,500
1,300
>8,500
>8,500
1,500
7,900
>8,500
2,200
2,300
2,800
>8,500
4,600
2,400
190
130
120
666
160
1686
2056
190
75
<1C
160
546
1906 +
>340 +
52 +
>338 +
>340 +
59 +
320 +
>340 +
87 +
91 +
110 +
>340 +
190 +
98 +
(continued)
-------
TABLE D.14-8 (continued)
H
00
Peak
number
27
28
29
30
31
32
33
34
35
36
37
t , min
26.57
28.04
30.16
31.44
34.08
38.50
41.10
44.10
47.18
50.55
56.20
Log tr
1.4244
1.4478
1.4794.
1.4975"
1.5325
1.5855
1.6138
1.6444
1.6738
1.7037
1.7497
Calcd.
log P
6.35
6.47
6.64
6.74
6.92
7.20
7.35
7.51
7.67
7.82
7.07
Peak
height,
cm
5.1
6.8
6.4
5.1
3.6
2.4
2.5
1.7
1.4
1.0
0.4
Adjusted
peak
he ight ,
cm
440
580
550
440
310
210
210
150
120
85
34
Estimated
, concentration Positive
FSD, . index, or
% ppb negative
2,900
3,900
3,600
2,900
2,000
1,400
1,400
970
800
570
230
i
120 + •
160 + !
150 +
120 +
82 +
55 +
57 +
39 +
32 +
23 +
9 +
Retention time.
Percent full-scale deflection.
A positive response is defined as log P ^3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.14-9. CORRELATION3 OF BIOACCUMULATION POTENTIALb AND
GC/MS ANALYSIS TEST RESULTS: PLANT B149S
Organic compounds identified by Molecular Literature
GC/MS analysis formula log P value [1]
Actual sample
log P values
by HPLC method
(Naphthalene)0 C10H8 3.59
Methyl naphthalene C11H10 3.86
Tetrachlorophenol C6H2C140 4.0 ^
Dimethyl naphthalene ,C12H12 4.26 '
(1,7-Dimethyl naphthalene)0 C12H12 4.44
Trimethyl naphthalene ciaHi4 4.73
Pentachlorophenol C6HC150 5.01 '
Pentyl cyclohexane CX1H22 -
1 .I'-Biphenyl-ol C12H100
3.63
3.79
3.93
4.25°
4.49
4.67
4.93
5.02d
5.23
5.38
5.56
5.75
5.90
6.18
6.35
6.47
6.64
6.74
6.92
7.20
7.35
7.51
7.67
7.82
8.07
Potential correlation is defined as log P values agreeing ±0.05 units.
Data in this table are only for compounds 'which may bioaccumulate
(log P 53.5 and £25% FSD).
GTentative identification based solely on log P values and similar
compounds found in sample .
"potential correlation.
60ther values reported are 4.31, 4.37, 4.38, 4.40, 4.42. 4.44.
Other values reported are 3.81, 5.12, 5.86.
^No literature log P found.
[1] Hausch, C Computerized Printout of Log P Values by Increasing
Molecular Carbon Content and Increasing Log P, 1980.
D.14-14
-------
TABLE D.14-10. CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B149S IN
STATIC, UNAERATED ARTIFICIAL SEAWATER
Hour
24
48
72
LCso ,
% effluent
17
3
<3
95% confidence limits,
% effluent
12-25
b
-
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based on
nominal concentrations of test mate-
rial in artificial seawater. pH
and dissolved oxygen remained with-
in acceptable ranges throughout the
tests.
aAssay performed by EG&G Bionomics,
Pensacola, Florida.
Confidence limits not calculated.
D.14.3 Data Interpretation
D.14.3.1 Evaluation of Protocol Performance—
The effluent from Plant B149S was found to be highly toxic to
mysid shrimp (LC50 <3% at 72 hours). Since the value of TOC was
50 mg/L versus a 50 mg/L decision point, further analysis by GC/
MS and bioaccumulation materials fractionation and identification
was deemed necessary. Numerous organic compounds were present,
including several naphthalenic and phenolic compounds. The trace
metals analyses indicated high concentrations of chromium, manga-
nese, copper, and lead, all of which are of potential toxicologi-
cal significance.
D.14.3.2 Protocol Changes Suggested for Plant B149S—
No changes suggested.
D.14-15
-------
D.14.3.3 Potential Sources of Chemical Toxicity—
Potential sources of chemical toxicity include the numerous
organic compounds present and the high concentrations of several
trace metals.
D.14-16
-------
D.15 PLANT A101: SYNTHETIC ORGANIC FIBERS MANUFACTURE
D.15.1 Process Description
Plant A101 produces synthetic organic fibers in a form suitable
for further manufacturing on looms and knitting machines (SIC 2824
and 2299).
Water use at the site is shown in Figure D.15-1. Wastewater
originates from several sources including production processes;
boiler blowdown, and research and development operations for new
products. Sanitary and process wastes are combined in the waste-
water treatment facility, shown diagrammatically in Figure D.15-2.
Dyeing process wastewater is clarified and then is combined with
the chemical process wastewater in the first of two equalization
lagoons. The second releases the wastewater to two clarifiers in
parallel which are followed by a settling lagoon. The overflow
from this lagoon is pumped to two parallel trickling filters
which are subsequently followed by further clarification and
sedimentation before combination with surface runoff and non-
contact cooling water, which is stored in an adjacent firepond.
Sludge from the clarifiers and sedimentation lagoons is sent to a
thickener and then stored in a sludge drying lagoon. Wastewater
retention time is approximately 1.5 days. The discharge totals
625,000 gallons of water daily. Sanitary wastewater, which is
combined with the chemical wastewater just before the trickling
filters, totals 30,000 gal/day. Sampling was conducted before
the noncontact and surface waters were combined with the treated •
effluent.
D.15-1
-------
WATER
SUPPLY
RAW MATERIALS
,
RESEARCH
AND
DEVELOPMENT
FUEL
*
STEAM
PLANT
SANITARY. DYE SANITARY, DYE SANITARY AND
AND AND CHEMICAL WASTES
CHEMI CAL WASTES CHEMI CAL WASTES 1
1 t 1
V
V
TRE
i
(ASTE
I/ATER
ATMENT
i SAMPLING POINT
,
OFFICES
SANITARY WASTE
NONCONTACT COOLING WATER
DISCHARGE
Figure D.15-1.
Water flow diagram for Plant A101:
synthetic fibers manufacturing.
NCNCOMACI
COOLING
WATER
RAIN
RUNOFF
_ SEDIMENTATION
SAMPLING
POINT
y~x
/SECONDARY^
ICURIFIER J
LAGOON
— DISCHARGE
Figure D.15-2.
Waste treatment diagram for Plant A101
synthetic fibers manufacturing.
D.15-2
-------
D.15.2 Analysis Results, Plant A101
The .following tables in this section present the data collected
for Plant A101.
TABLE D.15-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT A101
Temper- Dissolved
Flow ature, oxygen,
m
3/d
15,
(4
(MGD)
520a
-1)
pH
7.6
°C
25
(78
(°F)
.6
-0)
mg/L
7.2
Comments
Plant operating normally; effluent
clear
a
Instantaneous measurement.
TABLE D.I5-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT A101
(mg/L, except as noted)
Detection
Species Concentration limit
BOD5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (ug/L)
Filtered mercury (pg/L)
Chemical oxygen demand
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
<6
2
<0.020
<0.010
12.7
<0.20
<0.20
53.2
2.37
2.19
0.095
2.4
34.0
130
<25
0.295
0.24
0.289
6
1.0
0.020
0.010
0.1
0.20
0.20
NR*
NR
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
aNo detection limit reported.
D.15-3
-------
TABLE D.15-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
A101, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected
None detected
Background correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
D.15-4
-------
TABLE D.15-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT A101
in
in
Concentration
in each
fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeablea ,
% Extractable
% Chromatographable ,
% GRAV Chromatographable
Acid
0.25
ND
ND
Base/
neutral
1.50
ND
0.08
Total
2
1.75
ND
0.08
ND
ND
87.5
4.0
4.6
a% of carbon purgeable = Total mass of purgeables x
b% of carbon extractable = TC°TQCGRAV x 100
c% of carbon Chromatographable = TC°ToCTCG
100
of grav Chromatographable = GRAV x 100
&
"ND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.I5-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT A101, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3.0
98.0
1,420
19.6
<1.0
4,310
<2.0
<6.0
<8.0
14.0
226
15.8
8,790
1,300
12.0
<10.0
293,000
<15.0
<70.0
<40.0
69.4
<25.0
<5.0
<5.0
231
aData not reported.
D.15-6
-------
TABLE D.15-6.
BI©ACCUMULATION POTENTIAL TEST RESULTS,
PLANT A101 EFFLUENT
i
v]
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
t , min
1.41
1.92
3.02
3.43
3.72
3.92
4.50
5.06
5.75
6.80
7.55
8.38
8.75
9.46
12.50
13.30
16.25
Log tr
0.1492
0.2833
0.4800
0.5353
0.5705
0.5933
0.6532
0.7042
0.7597
0.8325
0.8779
0.9232
0.9420
0.9759
1.0969
1.1239
1.2109
Peak
Calcd. height,
log P cm
-0.30
0.34
1.27
1.54
1.70
1.81
2.10
2.34
2.60
2.95
3.17
3.38
3.47
3.63
4.21
4.33
4.75
0.8
1.5
0.8
1.3
1.7
3.3
0.7
2.7
0.5
6.8
1.4
0.3
0.3
6.3
0.1
0.1
0.1
Adjusted
peak
height,
cm
73
140
73
120
150
300
64
250
46
620
130
27
27
570
9
9
9
Estimated
, concentration
FSD , index ,
% ppb
490
910
490
790,
1,000
2,000
430
1,600
300
4,100
850
180
180
3,800
61
61
61
<16
36
176
32
41
346
ioe
646
12
ioe
34
2e
26
<16
2
<16
2
Positive
negative
.
_
-
_
.»
_
_ i
-
-
-
-
-
-
+
+
+
+ '
a
Retention time.
Percent full-scale deflection.
*%
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.15-7. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT A101
LC50 value,
Time % of neat effluent
24-hour -b
48-hour
72-hour
96-hour >100
aAssay performed by State
Water Control Board, Com-
monwealth of Virginia.
Data not reported.
D.15.3 Data Interpretation
D.15.3.1 Evaluation of Protocol Performance—
Plant A101 produced an effluent without any measurable toxicity.
As per protocol, bioaccumulation fractionation yielded only four
peaks, all with very low estimated concentrations. This, com-
bined with a TOC of only 2 mg/L, resulted in termination of
further analytical characterization of this effluent.
D.15.3.2 Protocol Changes Suggested for Plant A101--
No protocol changes are suggested.
D.15.3.3 Potential Sources of Chemical Toxicity—
No potential sources of toxicity could be identified.
D.15-8
-------
D.16 PLANT B111D: TOBACCO PROCESSING
D.16.1 Process Description
Plant B111D manufactures cigarettes and cigars from tobacco, wood
pulp (paper manufacturing), and activated carbon (SIC 2111 and
2121).
Figure D.16-1 is a wastewater treatment diagram for the plant.
The sanitary waste (10,000 gallons per day) discharges to a
stabilization pond, and the discharge is chlorinated prior to
being mixed with the plant discharge. The industrial wastewater
plant consists of a primary clarifier, two aeration lagoons, and
a secondary clarifier with activated sludge returned to the
aeration lagoons. Retention times are estimated to be 0.3 days
in the primary clarifier, 11.9 days in the aerated lagoons, and
0.4 days in the secondary clarifier. The total plant flow rate
is 1.128 MGD.
SANITARY;
WASTE
DISCHARGE
PLANT
WASTE
Figure D.16-1. Wastewater treatment diagram for
Plant B111D: tobacco processing.
D.16-1
-------
D.16.2 Analysis Results, Plant B111D
The following tables in this section present the data collected
for Plant B111D.
TABLE D.16-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B111D
Temper- Dissolved
Flow ature, oxygen,
m3/d (MOD) pH °C (°F) mq/L Comments
4,920a
(1.3)
7.7 17.0
(62.6)
9.0 Plant operating normally;
in color
effluent brown
a
Instantaneous measurement.
TABLE D.16-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B111D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (\ig/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Color (true color units)
19.5
56
<0.020
<0.010
127
<0.20
<0.20
7.20
3.09
1.87
0.3
63.0
29.5
8.4
1.60
2.35
19.1
500
6
1.0
0.020
0.010
0.1
0.20
0.20
NR*
NR
0.04
0.25
0.5
1.0
2.5
0.007
NR
NR
NR
detection limit reported.
D.16-2
-------
TABLE D.16-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B111D, CORRECTED FOR BACKGROUND
Retention Concentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
27.5 Toluene <10d
Total concentration6 <10
Background correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
GQuantitated relative to area of major ion of
authentic standards.
10 |jg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.16-3
-------
TABLE D.16-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B111D
H
O>
I
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeablea ,
% Extractable0
% Chromatographable ,
% GRAV Chromatographable
Concentration in each fraction, mg/L
Extractable
Base/
Acid neutral Total
56
2.93 1.40 4.30
0.02 0.04 0.06
0.78 0.23 1.01
ND
7.8
1.9
18.0
a0, of rnrhnn nurorihlr _ Total mass
/o \)i <_.ul. JJOIl pUiycdij-Ltr — ~ — ~
b TCO "t* GRJ
% of carbon cxtr actable - TOC
c TCC
/> OJL L,aI.iJvJIl t^IliUllla UUyJL clJJIlciIJit;
70 of grav Chromatographable = -._..„
of purgeables _ ino
TOC
"~ X 100
j + TCG inn
TOC X 10°
x 100
'ND indicates total concentration is less than 0.01 mg/L,
-------
TABLE D.16-5
ICAP METALS ANALYZED BY EPA
REGION V, PLANT B111D, \ig/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
5.77
250
<80.0
48.0
<1.0
56,900
<2.0
<6.0
42.0
20.2
840
26.0
120,000
10,500
243
. <10.0
31,500
40.0
104
88.0
203
<25.0
22.9
<5.0
<40.0
not reported.
D.16-5
-------
TABLE D.16-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B111D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
t , min Log t
r' * r
1.34
1.88
2.10
2.40
3.40
3.67
3.84
4.19
5.04
6.05
6.47
7.40
8.28
11.06
13.07
36.12
0.1271
0.2742
0.3222
0.3802
0.5315
0.5647
0 . 5843
0.6222
0.7024
0.7818
0.8109
0.8692
0.9180
1.0438
1.1163
1.5577
Calcd.
log P
-0.54
0.23
0.48
0.78
1.56
1.73
1.84
2.04
2.45
2.86
3.02
3.32
3.57
4.23
4.60
6.90
Peak
height,
cm
0.2
4.7
3.0
2.6
10.2
>15
6.0
5.3
3.3
8.4
1.9
1.3
7.3
0.4
0.2
0.1
Adjusted
peak
height,
cm
17
400
260
220
870
>1,300
510
450
280
720
160
110
620
34
17
9
Estimated
. concentration
FSD , index ,
% ppb
110,
rt
2,700
1,700
1,500
5,800
>8,500
3,400
3,000
1,900
4,800
1,100
740
4,200
230
110
57
5
110
68
59
230
>285e
140
120
75
506
43
30
20C
9
5
2
Positive
or
negative
-
-
-
-
-
-
-
-
-
-
-
-t-
+
+
+
a
Retention time.
Percent full-scale deflection.
•»
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.16-7. CALCULATED LC5o'S FOR MYSID SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B111D IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
LCso /
Hour percent effluent
24
48
72
96
>100
>100
>100
>100
95% Confidence limits,
percent effluent
b
~b
"b
~b
Note: Salinity was 24 o/oo and temperature was
22 °C. Values were based on nominal concen-
trations of test material in artificial
seawater. pH and dissolved oxygen remained
within acceptable ranges throughout the
tests.
aAssay performed by EG&G Bionomic, Pensacola,
Florida.
Confidence limits not calculated due to LC50
D.16.3 Data Interpretation
D. 16. 3.1 Evaluation of Protocol Performance —
Effluent from plant B111D was non-toxic to mysid shrimp at all
concentrations. Protocol dictated screening for bioaccumulative
compounds, of which four peak were evaluated as positive. Fur-
ther sample analysis was terminated at this point due to the
relatively low estimated concentrations of the four detected
peaks .
In a case such as this, the protocol did provide efficiency, in
that unnecessary costly analytical characterization was not
pursued due to the relatively low indicated toxicity and the
absence of large concentrations of bioaccumulative species.
D.16.3. 2 Protocol Changes Suggested for Plant B111D —
No changes are suggested.
D.16-7
-------
D.16.3.3 Potential Sources of Chemical Toxicity—
Due to the nontoxic nature of the effluent for plant B111D, no
toxic compounds identified.
D.16-8
-------
D.17 PLANT B113D: NYLON MANUFACTURE
D.17.1 Process Description
Plant B113D is a chemical manufacturer producing synthetic resins,
plastics, nonvulcanizable elastomers, and synthetic organic fibers
(Nylon 66) in the form of monofilament, yarn, staple, or tow
suitable for further processing (SIC 2821 and 2824).
Figure D.17-1 is a wastewater treatment diagram for the plant.
Wastewater generated by the process areas is collected for neutral-
ization.with lime, aerated in lagoons (about 2 days retention),
then passes to a polishing pond to remove settleable material
before final discharge. Overall retention time is 3 to 5 days.
Septic tank effluent enters at the aeration lagoons, and non-
contact cooling water is used to dilute the final discharge.
D.17.2 Analysis Results, Plant B113D
The following tables in this section present the data collected
for Plant B113D.
WASTE
WATER
SEPTIC
TANK
EFFLUENT
NON-CONTACT
COOLING
WATER
»
NEUTRALIZATION
CELL
i
AERATED
LAGOONS
-
POLISHING
POND
•SAMPLE
POINT
DISCHARGE
(UPSTREAM FROM
THE NON-CONTACT
COOLING WATER)
OUTFALL 002
Figure D.17-1.
Wastewater treatment diagram for Plant B113D:
Nylon production.
D.17-1
-------
TABLE D.17-1. SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B113D
Temper- Dissolved
Flow, ature, oxygen,
m
3/d
24.
(6-
(MGD)
870a
57)
pH
8.3
°C
17
(63
(°F)
.5
-5)
mg/L
13.5
Comments
Plant operating normally;
effluent clear
Instantaneous measurement.
TABLE D.17-2. WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B113D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (jjg/L)
Filtered mercury (ug/L)
Chemical oxygen demand
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
16.2
8
<0.020
<0.010
42
• <0.20
<0.20
209
17.8
9.35
0.288
<2.5
440
1,230
<250
1.5
2.24
1.52
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
NR
0.04
2.5
0.5
1.0
250
0.007
NR
NR '
aNo detection limit reported
D.17-2
-------
TABLE D.17-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B113D, CORRECTED FOR BACKGROUNDa
Retention Concentration,
time, min Compound pg/L
Internal Standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected
12.6 Trichlorofuloromethane 20
Total Concentration 20
aBackground corrected based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
°Quantitated relative to area of major ion of
authentic standards.
Summation of concentrations of detected compounds,
excluding standards.
D.17-3
-------
TABLE D.17-4. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B113D
Concentration
in each
fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
0/0 Purgeablea .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
Acid
0.52
NDe
ND
Base/
neutral
1.60
0.02
0.15
Total
8
2.10
0.02
0.15
0.02
0.3
26.5
2.1
7.1
a% of carbon purgeable = Total mass ofurgeables x
b% of carbon extractable = TC°TQCGRAV x 100
c TCO
% of carbon Chromatographable =
TCG
TQC
x 100
ND indicates total concentration is less than 0.01 mg/L.
TCG
of grav Chromatographable = a x 10°
-------
TABLE D.17-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B113D, [tg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<3.0
207
93
46.7
, <1.0
554,000
4.32
<6.0
32.9
21.9
611
29.4
5,000
9,300
450
90.9
293,000
52.4
<70.0
<40.0
381
<25.0
106
6.97
<40
aData not reported.
D.17-5
-------
TABLE D.17-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B113D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
a
t , mm
1.83
2.06
3.23
3.56
3.91
4.34
5.25
5.82
6.42
7.24
7.93
8.94
9.53
10.01
15.30
Log tr
0.2625
0.3139
0.5092
0.5515
0.5922
0.6375
0.7202
0.7649
0.8075
0.8597
0.8993
0.9513
0.9791
1.0004
1.1847
Calcd.
log P
0.22
0.49
1.52
1.75
1.96
2.20
2.64
2.87
3.10
3.37
3.58
3.86
4.00
4.11
5.09
Peak
height,
cm
2.9
1.0
3.5
8.9
1.8
2.7
0.9
7.1
1.2
1.0
6.8
0.2
0.6
2.2
0.25
Adjusted
peak
height,
cm
250
85
300
760
150
230
77
610
100
85
580
17
51
190
21
Estimated
, concentration Positive
FSD, index, or
% ppb negative
l,700d
570
2,000
5,100
1,000
1,500
510
4,000
680
570
3,700
110
340
1,300
140
66
23
786
1456
396
61
20
50e
27
18C
30e
5 +
14 +
50 +
6 -1-
Retention time.
Percent full-scale deflection
"A positive response is defined as log PS3.5 with % FSD >25.
See Appendix C for an explanation of FSD in excess of 100.
&
"Corrected for background interference.
-------
TABLE D.17-7. FATHEAD MINNOW BIOASSAY RESULTS3
FOR PLANT B113D
LC50 value,
Time % of neat effluent
24-hour -b
48-hour
7.2-hour
96-hour >100
aAssay performed by State
Water Control Board,
Commonwealth of Virginia.
Data not reported.
D.17.3 Data Interpretation
D.17.3.1 Evaluation of Protocol Performance—
Effluent from plant B113D was tested and yielded no measurable tox-
icity to fathead minnows. Bioaccumulation fractionation revealed
five peaks with estimated concentrations of 50, 30, 15, 5, and
6 ppb respectively. The low TOC valve, 8 mg/L, thus directed
termination of further costly analytical characterization of this
effluent.
D.17.3.2 Protocol Changes Suggested for Plant B113D—
No protocol changes are suggested.
D.17.3.3 Potential Sources of Chemical Toxicity—
No potential sources of toxicity were identified.
D.17-7
-------
5.18 PLANT B124D - TOBACCO PROCESSING
D.18.1 Process Description
Plant B124D is a manufacturing facility which produces a recon-
stituted tobacco product from tobacco materials (SIC 2111). The
process is proprietary.
Figure D.18-1 is a wastewater treatment diagram for the plant.
Wastewater generated by processing goes through extended aeration,
activated sludge, pretreatment (bar rack, aerated grit chamber and
coarse screen), primary treatment in three 35 ft diameter by 9 ft
deep clarifiers and a 300,000 gallon equalization basin, secondary
treatment in three 2.5 MG aeration basins and four 40 ft diameter
by 8 ft deep clarifiers, chlorination in two rectangular baffled
contact chambers, filtration by three multimedia gravity filters
with a 1.5 MGD capacity and three dual media pressure filters with
1.0 MGD capacity, and then final discharge. Sludge thickening
and dewatering is done with two 35 ft diameter by 8 ft deep clari-
fiers and two vacuum coil filters with a belt filter press. Hy-
draulic retention time through the treatment system is 5.6 days
(134.8 hr), divided as follows: pretreatment-0.2 hours, primary-
3.4 hours, secondary-130.2 hours, chlorination-0.8 hours, and
filtration-0.2 hours. The total plant effluent flow rate is
1,000 gpm, or 1.44 MGD.
D.18.2 Analysis Results, Plant B124D
The following tables in this section present the data collected
for Plant B124D.
D.18-1
-------
WASTE
WATER
EXTENDED
AERATION
ACTIVATED
SLUDGE
/ ,
V <
O
00
I
Is)
SAMPLING
POINT
DISCHARGE
Figure D.18-1.
Wastewater treatment diagram for Plant B124D:
tobacco processing.
-------
TABLE D.18-1. SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B124D
Temper- Dissolved
Flow, ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments
5,300a 6.9 19.0 4.3 Plant operating normally,- effluent brown
(1.4) (6.62) in color and smelled of chlorine.
Instantaneous measurement.
TABLE D.18-2. WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B124D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (ug/L)
Filtered mercury (ug/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Color (true color units)
13.8
64
0.183
<0.010
8.7
<0.20
<0.20
13.1
0.376
87.4
<2.5
160
54.0
411
16.4
14.6
17.2
300
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
2.5
0.5
1.0
2.5
0.007
NR
NR
NR
aNo detection limit reported.
D.18-3
-------
TABLE D.18-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B124D, CORRECTED FOR BACKGROUNDa
Retention Concentration,
time, min Compound
Internal Standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected0
10.5
15.1
17.9
27.5
Methylene chloride
Chloroform
Dichlorobromome thane
Toluene
Total concentration6
<10d
296,
<10d
<326
Background corrected based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
°Quantitated relative to area of major ion of
authentic standards.
10|jg/L is quantitation limit of computerized identi-
fication system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.18-4
-------
TABLE D.18-4. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B124D
oo
i
01
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
0/0 Purgeablea .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
Concentration in each fraction, mg/L
Extractable
Base/
Acid neutral Total
64
3.12 1.80 4.90
0.10 0.09 0.19
0.36 0.25 0.61
<0.33
0.5
7.9
1.3
12.4
a», f rnrbon nurarnhlr Total mass Of
b0 TCO + GRAV
/0 of carbon cxtr actable TOC 3
C TCO 4-
V nf r-aT-V^on rma't-ortT-ar»Via1-»l «» — .
purgeables _ 1on
^
TCG „ ,nn
of grav Chromatographable =
TOC
x 100
-------
TABLE D.18-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B124D,
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
3.95
126
124
87.9
<1.0
86,300
<2.0
<6.0
26.6
29.7
<80.0
47.5
431,000
30,600
31.6
17.2
40,500
<15
<70
<40
251
<25
<5.0
<5.0
366
aData not reported.
D.18-6
-------
TABLE D.18-6.
oo
i
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B124D EFFLUENT
-
Peak
number
1
2
3
4
5
6
7
8
9
t , min
1.94
2.19
3.68
4.48
4.75
6.09
7.38
8.33
11.28
Log tr
0.2878
0.3263
0.5658
0.6513
0.6767
0.7846
0.8681
0.9206
1.0523
Calcd.
log P
0.30
0.50
1.74
2.19
2.32
2.88
3.31
3.59
4.27
Peak
height,
cm
8.1
5.0
10.7
7.3
9.8
1.9
8.1
0.6
Adjusted
peak
height,
cm
700
430
>1,300
920
630
840
160
700
52
Estimated
, concentration Positive
FSD,
%•
4,600d
2,900
>8,600
6,100
4,200
5,600
1,100
4,600
340
index ,
ppb
190
110
>285
2456
170
80e
44
40e
14
or i
negative
-
_
-
-
-
^ i
+
+
a,
Retention time.
Percent full-scale deflection
"A positive response is defined as lop P§3.5 with % FSD >25.
See Appendix C for an explanation of FSD in excess of 100.
"Corrected for background interference.
-------
TABLE D.18-7. CALCULATED LC5o'S FOR MYSID SHRIMP (NYSfDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B124D IN
STATIC, UNAERATED ARTIFICIAL SEAWATER
Hour
24
48
72
96
LC50 ,
_% effluent
71
71
71
59
95% confidence limits,
% effluent
50
50
50
48
- 100
- 100
- 100
- 74
Note: Salinity was 25 o/oo and temperature
was 22°C. Values were based on nom-
inal concentrations of test material
in artificial seawater. pH and dis-
solved oxygen remained within ac-
ceptable ranges throughout the
tests.
aAssay performed by E.G. & G. Bionomics;
Pensacola, Florida.
D.18.3 Data interpretation
D.18.3.1 Evaluation of Protocol Performance—
The wastewater effluent from Plant B124D was of moderate to low
toxicity to mysid shrimp (LC50 = 59%, 96 hr.). Inorganic and
ionic analyses indicated potential toxicological effects due to
(1) free chlorine from, the wastewater treatment process, (2)
cyanide at the 0.183 mg/L level, and (3) copper and zinc at
29.7 (jg/L and 366 ug/L concentrations, respectively. Even
though the TOC level of 64 mg/L exceeded the protocol decision
point of 50 mg/L it was decided not to conduct GC/MS analysis.
The purgeable organic content in the wastewater indicated a
chloroform concentration of 286 pg/L.
The protocol succeeded in identifying several candidates for the
observed toxicity.
D.18-8
-------
D.18.3.2 Protocol Changes Suggested for Plant B124D—
No changes in protocol are suggested.
D.18.3.3 Potential Sources of Chemical Toxicity—
Candidate sources for the toxicity observed in Plant B124D waste-
water effluent include free chlorine, cyanide ion at 0.183 mg/L,
copper at 29.7 pg/L and zinc at 366 pg/L concentrations. The
presence of 296 H9/L of chloroform could also contribute to the
toxicity.
D.18-9
-------
D.19 PLANT C151D: COMMERCIAL ELECTRIC POWER GENERATION
D.19.1 Process Description
Plant C151D is a commercial electric power generator (SIC 4911).
Figure D.19-1 is a water flow and wastewater treatment diagram
for the plant. Wastewater is generated from boiler blowdown,
evaporator blowdown, air preheater wash water, demineralizer
wastes, compressor and cooling tower blowdown, water treatment
plant effluent, oily wastes basin, and boiler cleaning units.
These wastewaters go to a master sump, intermediate basin, or ash
sluice units before going to the ash pond. A metals treatment
basin is operated on a batch basis where lime is added to adjust
the pH. From the ash pond the effluent is mixed with noncontact
cooling water and storm runoff before final discharge.
D.19-1
-------
O.U5MGD
COUNTY
WATER '
COUNTY
WATER '
COUNTY
WATER •
JAMES
RIVER
JAMES
RIVER'
COUNTY
WATER "
COUNTY
WATER
COUNTY
WATER "
COUNTY
WATER "
RAINFALL-
COUNTY
WATER —
JAMES
RIVER
COUNTY
WATER
JAMES
RIVER
COUNTY
WATER
RAINFALL-
BOILER
SLOWDOWN
EVAPORATOR
SLOWDOWN
FLOOR
DRAINS
CHLORINE
BACKWASH
AIR PREHEAT
' WASH WATER
DEM1NERAUZER
" WASTES
COMPRESSOR.
. COOLING TOWER
SLOWDOWN
WATER
TREATMENT
" PLANT
EFFLUENTS
0.028 MGD
O.W33MGO
OM2MG°. MASTERS
A
i
____j
0.003 MGD
0.06MGD
i i
JAMES •"*
RIVER _
0.241 MGD
JAMES"*
RIVERA _
0.06 MGD
264. 18 MGD
JAMES"
RIVER
BACKWASH
«. UNIT 6
SCRE
BACK
:N
WASH UNIT 4
BACKWASH UNITS
001
•22.
m
CONDENSER
COOLING
WATER
UNITS 1-3
P 0.2084 MGD
DIKED 3.686MGOL ,
PORTION rTIiwD.
Of ASH POND | •*
0.03 MGD INTERMEDIATE
'*" BASIN
• OILY
WASTES
• BASIN
0.0216 MGD
3.456 MGD
I
BOILER
- CLEANING
UNITS 1-4
0.581 MGD ASH
UNIT
1
CE
51-4
l
y
6. 336 MGD
t !
METAL
. «» TREATMENT •«-/
BASIN
UME
ADDITION
(ADJUST pH)
y __ 0.309 MGD
RAINFALL
12.8MGD (SI
0.027MGD IS.I
BOILER
CLEANING
UNITS 5-6
5.17 MGD
ASH
SLUICE
UNITS 5-«
16.128 MGD
2.2 MGD
COUNTY
WATER
• COUNTY STP
JAMES
RIVER
COUNTY
WATER
U-
MISCELLANEOUS
LOSSES
•ATMOSPHERE
Figure D-19. Water flow and wastewater diagram for
Plant C151D: electric power production.
D.19-2
-------
D.19.2 Analysis Results, Plant C151D
The following tables in this section present the data collected
for Plant C151D. .
TABLE D.19-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C151D
Temper- Dissolved
Flow ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments
15,520a 6.5 17.5 13.4 Plant operating normally; slightly milky
(4.1) (63.5) effluent
Instantaneous measurement.
TABLE D.I9-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C151D
(mg/L, except as noted)
'Detection
Species Concentration limit
BODS
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (|jg/L)
Filtered mercury (ug/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Color (true color units)
<6
2
<0.020
0.010
18.7
<0.20
<0.20
0.283
0.045
0.230
0.5
20.0
89.6
<25
<0.007
0.065
0.015
5
6
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
NR
aNo detection limit reported.
D.19-3
-------
TABLE D.19-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C151D, CORRECTED FOR BACKGROUND3
Retention Concentration •,
time, min Compound jjg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected
None detected
Background correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
D.19-4
-------
TABLE D.19-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C151D
O
i
01
Concentration in each fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Acid
0.55
ND
0.06
Base/
neutral
1.2
ND
0.24
Total
2
1.75
ND
0.30
ND
Carbon balance
% Purgeable .
% Extractable0
% Chromatographable
% GRAV Chromatographable
ND
87.5
15.0
17.1
a% of carbon purgeable = Total mass of purgeables x 10Q
X WVx
b% of carbon extractable = TC°TQCGRAV x 100
c% of carbon Chromatographable = TC°TQCTCG x 100
.4 TCG
/£ of grav Chromatographable = -_." . x 100
VjKAV
eND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.19-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C151D,
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent. solids
<3.0
545
192
146
<1.0
29,900
<2.0
7.61
153
17.5
1,700
133
5,250
4,670
171
53.7
19,100
133
<70.0
145
380
<25.0
17.7
<5.0
<40.0
aData not reported.
D.19-6
-------
TABLE D.19-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C151D EFFLUENT
vO
I
-J
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
t ,a min
1.82
2.00
2.94
3.32
3.63
4.00
4.40
4.89
6.04
6.85
7.25
7.45
8.27
10.00
11.13
11.45
13.00
22.00
Log tr
0.2601
0.3010
0.4683
0.5211
0.5599
0.6021
0.6435
0.6893
0.7810
0.8357
0.8603
0.8722
0.9175
1.0000
1.0465
1.0588
1.1139
1.3424
Peak
Calcd. height,
log P cm
0.15
0.37
1.24
1.51
1.71
1.93
2.15
2.38
2.86
3.14
3.27
3.33
3.57
4.00
4.24
4.30
4.59
5.78
4.6
1.3
1.2
2.0
6.5
1.1
1.4
1.5
13.2
0.6
0.7
0.7
14.1
0.3
0.3
0.3
0.1
0.1
Adjusted
peak
height,
cm
790
220'
210
340
1,100
190
240
260
2,300
100
120
120
2,400
52
52
52
17
17
Estimated
, concentration
FSD, index,
% ppb
5,300d
1,500
1,400
2,300
7,500
1,300
1,600
1,700
1,500
690
800
800
16,000
340
340
340
110
110
210
60
55
92
245e
45e
64
69
4706
28
25e
256
500e
14
14
14
5
5
Positive
or c
negative
w
. -
-
-
_
-
-
_
.-
-
_
_
+
+
+
+
+
+
a
Retention time.
Percent full-scale deflection.
"A positive response is defined as log P >3.5 with % FSD >25.
3
See Appendix C for an explanation on FSD in excess of 100.
Corrected for.background interference.
-------
TABLE D.19-7. CALCULATED LC50'S FOR MYSID SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE C151D IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER*
Hour percent effluent
24
48
72
96
>100
>100
>100
56
95% Confidence limits,
percent effluent
_b
-^
_k
_c
Note: Salinity was 25 o/oo and temperature was
22 °C. Values were based on nominal concen
trations of test material in artificial
seawater. pH and dissolved oxygen re-
mained within acceptable ranges throughout
the tests.
aAssay performed by EG&G Bionomic, Pensacola,
Florida.
Confidence limits not calculated due to LC50
GConfidence limits not calculated
D.19.3 Data Interpretation
D. 19. 3.1 Evaluation of Protocol Performance —
The effluent from plant C151D was found to be moderately toxic to
mysid shrimp (LC50 =56%; 96 hr). From the inorganic and ionic
analyses, the potential sources of toxicity are free chlorine
from the wastewater treatment plant, chromium at a 153 M9/L
concentration, and possibly zinc at 40 pg/L. Since the toxicity
was below the 50% LC50 decision point and the TOC was only 2 mg/L,
no GC/MS analyses were warrented and no further eludication of
the structure of bioaccumulative species was undertaken.
In this instance the protocol performed well in that unnecessary
organic species analyses were not conducted.
D.19-8
-------
D.19.3.2 Protocol Changes Suggested for Plant C151D—
No changes in the protocol are recommended for a plant of this
type. It might have been interesting, however, to identify the
structure of the major bioaccumulative peak (estimated at a
concentration of 0.5 ppm).
D.19.3.3 Potential Sources of Chemical Toxicity—
The primary sources of toxicity of the wastewater effluent from
plant C151D are presumed to be free chlorine from the wastewater
treatment plant and a chromium concentration of 153 ^g/L. A
potential secondary source is zinc at a 40 p/L concentration in
the effluent.
D.19-9
-------
D.20 PLANT C153D: CHEMICAL FERTILIZER-PRODUCTION
D.20.1 Process Description
Plant C153D is a chemical fertilizer producer specializing in
phosphate pulverization, acid treatment, acid reclamation, and
fertilizer blending. Triple superphosphate, normal superphos-
phate, ammoniated superphosphate, sulfuric acid, and potassium
silicofluoride are major products from this site (SIC 2874).
No wastewater treatment occurs at this plant. Cooling water and
.surface runoff are directed to a drainage ditch which takes the
effluent to a river.
D.20-1
-------
D.20.2 Analysis Results, Plant C153D
The fallowing tables in this section present the data collected
for Plant C153D.
TABLE D.20-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C153D
Temper- Dissolved
Flow ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L _ Comments
10,600a
(2.8)
7.2 22.5
(72.5)
8.2 Salinity =12.5 ppt; plant operating
normally; clear effluent
Instantaneous measurement.
TABLE D.20-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C153D
(mg/L, except as noted)
!Detection
Species Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (ng/L)
Total Kjeldahl nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
7
<0.020
0.010
231
<0.20
<0.20
0.542
400
1,200
3,000
2,500
0.054
0.081
0.058
1.0
0.020
0.010
0.1
0.20
0.20
NRa
0.25
0.5
1.0
2,500
0.007
NR
NR
No detection limit reported.
D.20-2
-------
TABLE D.20-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C153D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detectedc
15.1 Chloroform <10d
Total concentration6 <10
aBackground correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
°Quantitated relative to area of major ion of
authentic standards.
10 pg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.20-3
-------
TABLE D.20-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C153D
to
o
Concentration
in each
fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeable3 .
% ExtractableD
% Chromatographable ,
% GRAV Chromatographable
Acid
0.28
ND
0.02
Base/
neutral
0.20
ND
0.03
Total
7
0.48
ND
0.05
<0.01
<0.1
6.9
0.7
10.4
a% of carbon purgeable = Total mass ofpurgeables x
^
b% of carbon extractable = TC°TQCGRAV x 100
c TCO + TCG
% of carbon Chromatographable =
TOC
x 10°
TCG
of grav Chromatographable = GRAV x 100
ND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.20-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C153D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V '
Y
Zn
Unfiltered Suspended
effluent solids
17
<400
2,640
32.7
<5
233,000
<10
<30
406
<30
1,490
112
196,000
630,000
127
<50
2,770,000
125
<350
<200
4,370
<75
55.5
<25
<200
not reported.
D.20-5
-------
TABLE D.20-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C153D EFFLUENT
to
o
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
a
t , mm Log t
r' * r
1.44
1.86
2.25
3.65
3.93
4.55
5.13
6.80
8.45
8.75
9.47
11.25
13.50
16.38
0.1584
0.2695
0.3522
0.5623
0 . 5944
0.6580
0.7101
0.8325
0.9269
0.9420
0.9764
1.0512
1.1303
1.2143
Adjusted
Peak peak
Calcd. height, height,
log P cm cm
-0.26
0.27
0.66
1.66
1.82
2.12
2.37
2.95
3.40
3.47
3.63
3.99
4.67
4.76
0.8
1.1
0.6
1.7
4.0
0.9
0.7
7.3
0.35
0.35
7.1
0.1
0.1
0.05
73
100
55
150
360
82
64
670
32
32
650
9
9
5
Estimated
. concentration
FSD , index ,
% ppb
490d
670
360
1,000
2,400
550
430
4,400
210
210
4,300
61
61
30
<1C
27
15
41
5le
22
15C
20C
4
4e
206
2
<16
1
Positive
or. c
negative
—
-
-
_
-
-
_
-
-
-
+
+
+
+
Retention time.
Percent full-scale deflection.
•»
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
"Corrected for background interference.
-------
TABLE D.20-7. SHEEPSHEAD MINNOW BIOASSAY RESULTS3
FOR PLANT C153D
LC50 value,
Time percent of neat effluent
24-hour -b
48-hour
72-hour
96-hour >100
aAssay performed by State Water
Control Board, Commonwealth of
Virginia.
Data not reported.
D.20.3 Data Interpretation
D.20.3.1 Evaluation of Protocol Performance—
The effluent from C153D had no measurable toxicity when tested
with the Sheepshead minnow. Bioaccumulation fractionation
revealed only four peaks, all with very low estimated
concentrations. The TOC value was only 7 M9/L» therefore, fur-
ther analytical characterization of this effluent was terminated.
D.20.3.2 Protocol Changes Suggested for Plant C153D—
No protocol changes are suggested for plant C153D.
D.20.3.3 Potential Sources of Chemical Toxicity—
No potential sources of chemical toxicity were identified.
D.20-7
-------
D.21 PLANT C154D: AMMONIATED FERTILIZER PRODUCTION
D.21.1 Process Description
Plant C154D is a chemical manufacturer producing ammoniated
fertilizers (SIC 2873). No wastewater is generated at this
plant, although storm water runoff and non-contact cooling water
are discharged. Figure D.21-1 is a water flow diagram for the
plant.
STORM WATER
RUNOFF
SAMPLING
POINT
~ _ DISCHARGE
NON-CONTACT
COOLING WATER
Figure D.21-1. Water flow diagram for Plant C154D:
ammoniated fertilizer production.
D.21-1
-------
D.21.2 Analysis Results, Plant C154D
The following tables in this section present the data collected
for Plant C154D.
TABLE D.21-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C154D
Temper- Dissolved
Flow ature,. oxygen,
m3/d (MGD) pH °C (°F) mg/L Comments
NA3 6.5 33.0
(91.4)
6.2 Salinity = 21 ppt; plant operating nor-
mally; effluent clear; sample taken
from effluent tank with rubber hose
Data not available; no reading taken.
TABLE D.21-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C154D
(mg/L, except as noted)
Detection
Species Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (jjg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride.
Sulfate
Sulfite
Dissolved phosphate (ortho)
7
<0.020
0.010
359
<0.20
<0.20
0.523
0.223
0.044
<25
13,400
2,200
<250
0.031
1.0
0.020
0.010
0.1
0.20
0.20
NR*
NR
0.04
25
0.5
1.0
250
0.007
detection limit reported.
D.21-2
-------
TABLE D.21-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C154D, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
14.3 1,1-Dichloroethane
-------
TABLE D.21-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C154D
O
to
H
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeablea .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
Concentration in each fraction, mg/L
Extractable
Base/
Acid neutral Total
7
0.25 0.25 0.50
ND 0.43 0.43
0.04 0.25 0.29
<0.02
<0.3
13.3
10.3
58.0
a0/ - rnrhnn -..-^..h-,-, Total mass
/o 01 carDon purgeaoj.e — —
c TCC
V f\f ^"»a T-V^r\Ti rf^Vi't"r\ma'f"/^rtT'aii^Vi aVkl ^ —
of purgeables _ inn
TOC X 10°
X inn
5 + TCG „ inn
TQC
•TV*1/"*
of grav Chromatographable = -PATT x 100
LrtxAV
indicates total concentration is less than 0.01 mg/L,
-------
TABLE D.21-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C154D,
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
<15
459
3,080
301
<5.0
273,000
<10
<30
306
<30
591
128
218,000
675,000
97.9
<50
3,090,000
<75
680
<200
5,110
<75
91.9
<25
<200
*Data not reported.
D.21-5
-------
TABLE D.21-6.
en
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C154D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
tf,a min Log tf
1.42
1.88
2.13
2.25
3.63
3.92
4.30
4.89
5.80
6.76
7.50
8.20
8.50
9.40
10.63
0.1523
0.2742
0.3284
0.3522
0.5599
0.5933
0.6335
0.6893
0.7634
0.8299
0.8751
0.9138
0.9294
0.9731
1.0265
Calcd.
log P
-0.28
0.30
0.56
0.67
1.66
1.82
2.01
2.27
2.62
2.94
3.16
3.34
3.41
3.62
3,87
Peak
height,
cm
0.5
1.0
0.6
0.4
1.7
5.7
1.2
4.4
0.5
7.0
0.6
0.3
0.3
•7.5
0.1
Adjusted
peak
height,
cm
46
91
55
36
150
520
110
400
46
640
55
27
27
680
9
Estimated
. concentration
FSD, index,
% ppb
300d
610
360
240
1,000
3,500
730
2,700
300
4,300
360
180
180
4,600
61
<1C
24
15
10
41
94C
29
110
12
ioe
15
26
2
306
2
Positive
or
negative0
_
-
-
-
-
-
-
-
-
.
-
-
• -
+
+
(continued)
-------
TABLE D.21-6 (continued)
ro
H
I
•v]
Peak
number
16
17
18
19
20
21
22
23
24
t , min
11.45
12.38
13.13
13.75
14.62
18.13
18.75
19.50
26.88
Log tr
1.0588
1.0927
1.1183
1.1383
1.1649
1.2584
1.2730
1.2900
1.4294
Calcd.
log P
4.03
4.19
4.31
4.41
4.53
4.98
5.05
5.13
5.79
Peak
height,
cm
0.1
0.1
0.2
0.2
0.7
0.4
0.4
0.35
0.2
Adjusted
peak
height,
cm
9
9
18
18
64
36
36
32
18
Estimated
. concentration Positive
FSD,
%
61
61
120
120
430
240
240
210
120
index ,
ppb
2
2
5
5
17
10
10
9
5
°r c
negative
+ '
+
+ !
1
+
+
+
+
+ .
Retention time.
Percent full-scale deflection.
•i
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
&
"Corrected for background interference.
-------
TABLE D.21-7,
CALCULATED LC5o'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE C154D IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
LCgo ,
Hour percent effluent
24
48
72
96
>100
21
12
5
95% Confidence limits,
percent effluent
_b
16-28
9-15
4-7
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal con-
centrations of test material in artifi-
cial seawater. pH and dissolved oxygen
remained within acceptable ranges through-
out the tests.
performed by EG&G Bionomics, Pensacola,
Florida.
^Confidence limits not calculated due to LC50
D.21.3 Data Interpretation
D.21.3.1 Evaluation of Protocol Performance—
The combined storm water run off and noncontact cooling water
from Plant C154D was found to be highly toxic to mysid shrimp
(LC50 = 5% at 96 hr). Since the TOC was only 7 mg/L, the deci-
sion tree dictated no further characterization of the small
organic content of this combined stream. The potential cause for
the high toxicity observed here could be attributed to either
chromium metal at a concentration of 136 pg/L and/or lead metal
at 680 |jg/L.
D.21.3.2 Protocol Changes Suggested for Plant C154D—
In a situation such as plant C154D where the source of toxicity
is questionable after collection of inorganic and ionic analyses
D.21-8
-------
data, it may be important to conduct GC/MS analyses of the organic
content even though the TOC is well below the 50 yg/L decision
point.
D.21.3.3 Possible Sources of Chemical Toxicity--
The two potential causes for the toxicity of the combined waters
from plant C154D are chromium and lead at concentrations of
306 jjg/L and 680 \tg/l>, respectively.
D.21-9
-------
D.22 PLANT C158D:
PLANT
COMBINED MUNICIPAL/INDUSTRIAL SEWAGE TREATMENT
D.22.1 Process Description
Plant C158D is a combined municipal/industrial sewage treatment
plant (SIC 4952).
Figure D.22-1 is a wastewater treatment diagram for the plant.
Influent is screened, goes through grit channels, and is pumped
to grit channels and more bar screens before entering the primary
clarifier lagoons. After primary clarifying, the wastewater is
aerated in tanks, then goes to secondary clarifiers, is disin-
fected with chlorine in a contact tank and finally discharged.
Sludge from the clarifiers is stored in lagoons, sent to thicken-
ing tanks, anaerobically digested in four digesters, sent to
sludge decant tanks and finally vacuum filtered. Wastewater
retention time is approximately 10-^11 hours. The total plant
effluent flow rate is 70 MGD.
INFLUENT
DISCHARGE
SLUDGE
DISPOSAL
Figure D.22-1.
Wastewater treatment diagram for Plant C158D:
municipal/industrial sewage treatment.
D.22-1
-------
D.22.2 Analysis Results. Plant C158D
The following tables in this section present the data collected
for Plant C158D.
TABLE D.22-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C158D
Temper- Dissolved
Flow ature, oxygen,
m3/d (MGD) pH °C (°F) mg/L Comments
264,950a
(70)
6.5 17.0
(62.6)
4.1 Chlorine =1.3 ppm; secondary plant;
rain (0.75 in.) previous night; efflu-
ent slightly grayish in color
a
Instantaneous measurement.
TABLE D.22-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C158D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (ug/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
Color (true color units)
7.8
16
0.110
<0.010
22.7
0.36
0.25
8.69
6.94
7.22
0.7
43.0
46.7
66.0
3.20
4.46
3.86
30
6
1.0
0.020
0.010
0.1
0.20
0.20
NRS
NR
0.04
0.25
0.5
1.0
2.5
0.007
NR
NR
NR
aNo detection limit reported.
D.22-2
-------
TABLE D.22-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C158D, CORRECTED FOR BACKGROUND
Retention Concentrati on,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
12.6 Trichlorofluoromethane <10
15.1 Chloroform <10
27.5 Toluene <10
Total concentration6 <30
Background correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
cQuantitated relative to area of major ion of
authentic standards.
10 (jg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.22-3
-------
TABLE D.22-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C158D
to
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeable3 .
% Extractable0
% Chromatographable ,
% GRAV chromatographable
Concentration in each fraction, mg/L
Extractable
Base/
Acid neutral Total
16
1.80 2.82 4.62
0.09 0.07 0.16
0.20 0.39 0.59
<0.003
<0.2
29.9
4.7
12.8
a Total mass
b" of clrbon Titrlctablc - TCO + GRj
^ TOC
/U of grav chromatographable = --VTV
of purgeables -,__
Xi nn
TOC X iu
x 100
-------
TABLE D.22-5,
ICAP METALS ANALYZED BY EPA
REGION V, PLANT C158D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
5.07
314
292
38.0
<1.0
24,500
<2.0
<6.0
44.0
47.0
680
<10.0
13,800
4,680
90.0
<10.0
44,800
16.0
<70.0
146
105
<25.0
<5.0
<5.0
67.0
not reported.
D.22-5
-------
TABLE D.22-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C158D EFFLUENT
o
ft
N)
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
t , min Log t
1.94
2.29
2.91
3.46
3.70
4.50
6.12
7.52
8.36
9.85
11.62
15.10
16.18
22.14
36.53
55.13
0.2878
0.3598
0.4639
0.5391
0.5682
0.6532
0.7868
0.8762
0.9222
0.9932
1.0653
1.1790
1.2090
1.3452
1.5626
1.7413
Peak
Calcd.- height,
log P cm
0.30
0.67
1.21
1.60
1.76
2.20
2.89
3.36
3.59
3.96
4.34
4.93
5.08
5.79
6.92
7.75
7.8
8.6
6.0
8.8
>15
4.9
8.0
4.6
8.1
0.8
0.3
0.2
0.2
0.2
0.5
0.05
Adjusted
peak
height,
cm
670
730
510
750
>1,300
420
680
390
690
68
26
18
18
18
43
4
Estimated.
. concentration Positive
FSD, index, or
% ppb negative
4,400d
4,900
3,400
5,000
>8,500
2,800
4,600
2,600
4,600
460
170
120
120
120
280
29
180
200
1356
200
>285e
1056
406
936
30C +
18 +
7 +
5 +
5 +
5 +
11 +
1 . +
a
Retention time.
Percent full-scale deflection.
•*
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.22-7. CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE C158D IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
Hour percent effluent
24
48
72
96
>100
>100
>100
89
95% Confidence limits
percent effluent
_b
~b
_c
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal con-
centrations of test material in artifi-
cial seawater. pH and dissolved oxygen
remained within acceptable ranges in
concentrations S25% effluent. Low dis-
solved oxygen in 50% and 100% effluent
would appear to be the major cause of
mortality in these test concentrations.
aAssay performed by EG&G Bionomics, Pensacola,
Florida.
Confidence limits not calculated due to LC50
£100%.
cConfidence limits not calculated.
D.22.3 Data Interpretation
D.22.3.1 Evaluation of Protocol Performance—
Plant C158D produced an effluent with low measurable toxicity,
i.e., 89% = LC50 for mysid shrimp at 96 hrs. Bioaccumulation
fractionation revealed 8 peaks; however, all were of low esti-
mated concentration and GC/MS was not performed on the collected
bioaccumulative peaks. The low TOC value, 16 pg/L, dictated
termination of further analytical characterization. However, a
specific source of toxicity was not positively identified.
D.22-7
-------
D.22.3.2 Protocol Changes Suggested for Plant C158D—
No protocol changes are suggested.
D.22.3.3 Potential Sources of Chemical Toxicity—
The protocol did not provide identification of a specific toxic
component. Zinc and copper, although borderline in a concentra-
tion to be concerned with, may have contributed to the low toxic-
ity observed with this effluent.
D.22-8
-------
D.23 PLANT C159D: PORK SLAUGHTER, PROCESSING, AND PACKAGING
D.23.1 Process Description
Plant C159D is a pork slaughter, processing, and packaging facility
(SIC 2011). Approximately 4,500 hogs are slaughtered daily to pro-
duce 4.5 million pounds/week of pork products.
Figure D.23-1 is a wastewater treatment diagram for the plant. The
wastewater is screened to remove raw waste solids before entering
anaerobic ponds. These ponds discharge through weirs to an aerobic
pond with both fixed and floating aerators. The overflow from the
aerated lagoon goes to clarifiers and sedimentation before chlori-
nation in a detention tank and final discharge through a parshall
flume. The underflow from the aerated pond goes to a sludge pond.
Wastewater retention time is about four to seven days. The total
plant effluent flow rate is 1.2 MGD.
WASTEWATER
SCREENS
DISCHARGE
SAMPLING
POINT
CHLORINE
DETENTION
TANK
Figure D.23-1. Wastewater treatment diagram for Plant C159D:
pork slaughter and packing.
D.23-1
-------
D.23.2. Analysis Results, Plant C159D
The following tables in this section present the data collected
for Plant C159D.
TABLE D.23-1. SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C159D
Temper- Dissolved
Flow, ature, oxygen,
m3/d (MGD) pH °C (°F) mg/L Comments
4,540a
(1.2)
7.6 19.5
(67.1)
6.5 Chlorine =1.8 ppm; Salinity =0.04 ppt;
plant operating normally; effluent
reddish brown.
Instantaneous measurement.
TABLE D.23-2. WET CHEMICAL, ION CHROMATOGRAPHY.,
AND NPDES RESULTS FOR PLANT C159D
(mg/L, except as noted)
Detection
Species Concentration limit
BOD 5
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (|jg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Total phosphorus
Total dissolved phosphorus .
14.6
18.0
0.044
<0.010
6.7
<0.20
<0.20
25.9
31.1
3.33
3.0
490
28
<25
11. oc
•~
6
1.0
0.020
0.010
0.1
0.20
0.20,
NR
NR
0.04
0.25
0.5
1.0
25
NR
NR
Samples exceeded required holding times,
No detection limit reported.
cSample improperly preserved.
D.23-2
-------
TABLE D.23-3. PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C159D, CORRECTED FOR BACKGROUND3
RetentionConcentration,
time, min Compound pg/L
Internal Standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics Detected0
15.1 Chloroform 16,
27.5 Toluene <10Q
Total concentration6 <26
aBackground correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
cQuantitated relative to area of major ion of
authentic standards.
10 M9/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.23-3
-------
TABLE D.23-4. ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C159D
to
co
I
Concentration
in each
fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
0/0 Purgeable3 .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
Acid
1.20
0.02
0.27
Base/
neutral
1.50
0.02
0.53
Total
18
2.70
0.04
0.80
<0.03
<0.2
15.2
4.7
29.6
a°' of carbon tmracablc - Total mass of
b* «f rnrhnn o,*r-nn1-nM n - TCO + GRAV v
purgeables inn
inn
TOC
c% of carbon Chromatographable = TC°TQCTCG x 100
TCG
Y0 of grav Chromatographable = GRA x 100
-------
TABLE D.23-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C159D, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
3.71
97
1,290
67.2
<1.0
7,700
2.50
7.46
13.6
14.7
510
10.0
26,500
500
33.6
<10.0
422,000
<15.0
<70.0
<40.0
31.0
<25.0
<5.0
<5.0
64.0
not reported.
D.23-5
-------
TABLE D.23-6.
K)
U>
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C159D EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
t , min
1.39
2.25
2.50
3.66
3.91
4.38
6.75
8.25
8.70
9.39
12.00
13.13
14.00
16.25
44.38
Log tf
0.1430
0 . 3522
0.3979
0.5635
0.5922
0.6415
0.8293
0.9165
0.9395
0.9727
1.0792
1.1183
1.1461
1.2109
1.6472
Calcd.
log P
-0.32
0.67
0.89
1.68
1.81
2.05
2.94
3.35
3.46
3.62
4.12
4.31
4.44
4.75
6.82
Peak
height,
cm
0.2
1.1
0.8
6.6
9.4
3.4
7.3
0.5
0.5
6.0
0.15
c
0.2
0.1
0.05
0.15
Adjusted
peak
height,
cm
18
100
73
600
860
.310
670
46
46
550
14
18
9
5
14
Estimated
. concentration
FSD, index,
% ppb
120d
670
490
4,000
5,700
2,100
4,400
300
300
3,600
91
120
61
30
91
<1C
27
19
1586
1846
766
20e
76
7e
<16
4
3
2
1
4
Positive
°r c
negative
—
-
-
_
_
w
-
-
-
+
+
+
+
+
+
a
Retention time.
Percent full-scale deflection
CA positive response is defined as lop P^3.5 with % FSD >25.
See Appendix C for an explanation of FSD in excess of 100.
Corrected for background interference.
-------
TABLE D. 23-10. FATHEAD MINNOW BIOASSAY RESULTS3 FOR PLANT C159D
Time %
24-Hour
48-Hour
7 2 -Hour
9 6 -Hour
LC50 value, 95%
of neat effluent Confidence limits
50
40
35
35
.0
.0
.0
.0
23
23
.0
.0
b
-
- 64
- 64
.«'
.8
.8
aAssay performed by State Water Control Board,
Commonwealth of Virginia.
95% Confidence limits not reported.
D.23.3 Data Interpretation
D.23.3.1 Evaluation of Protocol Performance—
The wastewater effluent from plant C159D was found to be of mod-
erate toxicity to fathead minnows (LC5o = 35.0%; 96 hr). The in-
organic and ionic analysis indicated three possible souces for
this toxicity including (1) free chlorine from wastewater treatment,
(2)ammonia nitrogen content of 31.1 mg/L (under basic conditions of
pH = 7.6), and zinc concentration of 64 Mg/L. Since the TOC was
18 mg/L and well below the protocol decision point of 50 mg/L, no
GC/MS analyses were conducted.
D.23.3.2 Protocol Changes Suggested for Plant C159D—
No suggested changes in the protocol.
/
D.23.3.3 Potential Sources of Chemical Toxicity—
Potential sources of the low toxicity of the wastewater effluent
from plant C159D include free chlorine, ammonia and zinc.
D.23-7
-------
D.24 PLANT C160D: RUBBER PRODUCT MANUFACTURE
D.24.1 Process Description
Plant C160D is a manufacturer of rubber products, mechanical rub-
ber goods, and elastomeric gasket materials (SIC 3069, 3293).
No wastewater treatment exists at this plant. Noncontact cooling
water from the molds and mills is combined with storm runoff water
before discharge, as shown in Figure D.24-1.
NON -CONTACT
COOLING WATER"
STORM
RUNOFF
SAMPLING
POINT
Q DISCHARGE
Figure D.24-1. Waterflow diagram for Plant C160D: miscellaneous
rubber product manufacturing.
D.24-1
-------
D.24.2 Analysis Results, Plant C160D
The following tables in this section present the data collected
for Plant C160D.
TABLE D.24-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C160D
temper- Dissolved
Flow ature, oxygen,
m3/d (MGD)
356a
(0.094)
pH
7.4
Op / Op \
26.5
(79.7)
mg/L
6.6
Comments
Plant operating normally;
effluent clear
Instantaneous measurement.
TABLE D.24-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C160D
(mg/L, except as noted)
Detection
Species Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (|jg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Total phosphorus
Total dissolved phosphorus
2
<0.020
<0.010
4.7
<0.20
<0.20
0.190
0.272
0.08
0.25
690
57.4
<25
0.053
0.074
0.057
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
25
0.007
NR
NR
aNo detection limit reported.
D.24-2
-------
TABLE D.24-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C160D, CORRECTED FOR BACKGROUND
/
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
10.5 Methylene chloride
-------
TABLE D.24-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C160D
to
Concentration in each fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Acid
0.33
NDe
ND
Base/
neutral
0.55
ND
0.02
!
Total
2
0.88
ND
0.02
<0.02
Carbon balance
% Purgeable3 .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
45.0
1.0
2.3
*% of carbon purgeable = Total mass of^purgeables x 10Q
b% of carbon extractable = TCOTQCGRAV x 100
c% of carbon Chromatographable = TCO * TCG x 100
grav Chromatographable =
TCG
TOC
x 100
"ND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.24-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C160D, \ig/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered Suspended
effluent solids
5.89
84.0
161.0
66.0
<1.0
116,000
5.1
<6.0
18.8
23.8
947
18.2
6,880
9,000
51.6
<10.0
74,000
<15.0
<70.0
<40.0
813
<25.0
<5.0
<5.0
50.0
aData not reported.
D.24-5
-------
D.25 PLANT B126S: INORGANIC PIGMENT MANUFACTURE
D.25.1 Process Description
Plant B126S is a manufacturer of inorganic pigments by chemical
precipitation and calcination batch processes (SIC 2816). Pro-
prietary cadmium pigments are prepared from cadmium, sulfur
compounds, and selenium plus additives and treating agents. This
site also prepares silica gel pigments from sodium silicate and
sulfuric acid plus additives.
Figure D.25-1 is a waterflow and wastewater treatment diagram for
the plant. Wastewater generated by the process contains metals
which are precipitated and filtered for two separate pigment
colors. Chemicals added are soda ash and trace amounts of ferric
sulfide. After filtration and solids recycle, all wastewaters
are combined before final discharge.
cm
WATER
SUPPLY
EVAPORATE
SAMPLING
POINT
DISCHARGE
Figure D.25-1
Water flow and waste treatment diagram for
Plant B126S: inorganic pigments production.
D.25-1
-------
D.25.2 Analysis Results, Plant B126S
The following tables in this section present the data collected
for Plant B126S.
TABLE D.25-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B126S
Temper- Dissolved
Flow ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments
No data taken by Maryland EPA
TABLE D.25-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B126S
(mg/L, except as noted)
Detection
Species Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (ng/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
6
<0.020
<0.010
101
<0.20
<0.20
300
348
1.56
0.7
24.0
1,050
<250
0.179
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR
0.04
0.25
0.5
1.0
250
NR
aNo detection limit reported.
D.25-2
-------
TABLE D.25-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B126S, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
21.2 Benzene 60,
27.5 Toluene <10a
Total concentration6 <70
aBackground correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
cQuantitated relative to area of major ion of
authentic standards.
10 pg/L is guantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.25-3
-------
TABLE D.25-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B126S
O
Concentration in each
Extractable
Base/
Analysis Acid neutral
TOC
GRAV 0.20 NDG
TCO ND 0.20
TCG 0.04 0.00
Purgeable organics
Carbon balance
% Purgeable3 .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
fraction, mg/L
Total
6
0.60
0.20
0.04
<0.07
13.3
4.0
6.7
a0, f r,rhnn niirnmhir Total mass of purgeables .__
bc/ f TCO + GRAV
/0 of carbon cxtractablc TQC x 100
TOC
^>/ f\f rtT-sirr r«ViT-rtnm+-nm-ar\Vi riKl a — AS**J v inn
indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.25-5,
ICAP METALS ANALYZED BY EPA
REGION V, PLANT B126S, [iq/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
7.51
94
120
20.3
<1.0
7,440
2,710
10.2
16.7
70.1
96
62.9
4,400
8,610
65.3
10.8
90,100
<15
97.2
85.5
91.5
<25
<5.0
<5.0
670
Suspended
solids
3.32
14
9
|.8
NDa
440
420
4.2
ND
62.2
16
7.5
1,440
910
9.8
0.8
23,400
ND
27.2
ND
1.5
ND
ND
ND
338
detected.
D.25-5
-------
TABLE D.25-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B126S EFFLUENT
N)
in
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
a
t , mm Log t
1.30
1.80
2.01
2.94
3.16
3.39
3.59
4.50
5.88
8.00
10.00
10.75
14.25
15.1
20.8
34.28
0.1139
0.2553
0.3032
0.4683
0.4997
0.5302
0.5551
0.6532
0.7694
0.9031
1.0000
1.0314
1.1538
1.1790
1.3181
1.5350
Adjusted
Peak peak
Calcd. height, height,
log P cm cm
-0.56
0.18
0.44
1.31
1.47
1.63
1.77
2.28
2.90
3.60
4.11
4.28
4.92
5.06
5.79
6.93
0.2
1.7
0.8
0.9
2.7
4.4
6.9
1.6
2.7
1.0
0.2
0.3
0.1
0.2
0.15
1.6
17
150
68
77
230
380
590
140
230
85
17
26
9
17
13
140
Estimated
, concentration Positive
FSD, index, or
% ppb negative
110d
970
460
510
1,500
2,500
3,900
910
1,500
570
110
170
57
110
85
910
<1C
39
18
21
61
100
1056
36
<1C
<1 +
5 +
7 +
2 +
5 +
3 +
36 +
.
Retention time.
Percent full-scale deflection.
"A positive response is defined as log P X3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
"Corrected for background interference.
-------
TABLE D.25-7. CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B126S IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
Hour
24
48
7-2
96
percent effluent
11
< 3
NDD
ND
95% Confidence limits
percent effluent
b
~b
—
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal con-
centrations of test material in artifi-
cial seawater. pH and dissolved oxygen
remained within acceptable ranges
throughout the tests.
aAssay performed by EG&G Bionomics, Pensacola,
Florida.
Confidence limits not calculated.
D.25.3 Data Interpretation
D.25.3.1 Evaluation of Protocol Performance—
For the case of plant B126S, preliminary data indicated a very
toxic effluent (LC50 = <3 after 48 hours by mysid shrimp). Appli-
cation of the decision tree logic to this plant resulted in a
decision to stop further analysis since the effluent ammonia anal-
ysis indicated a concentration of 348 mg/L and the TOC value was
only 6 mg/L. The presumption was made that the toxicity could be
attributed to ammonia alone. Later, the trace metal analyses
results were reported showing a cadmium concentration of 2,290 pg/L
which was three orders of magnitude greater than the average value
of cadmium for all 28 plants tested. If the metals data were avail-
able before the decision was made, the same decision would have
resulted especially since the TOC value was so low.
D.25-7
-------
D.25.3.2 Protocol Changes Suggested for Plant B126S—
No changes need be recommended since both the analytical method-
ology and the decision tree approach readily identified the
probable source of toxicity without the need for extensive com-
mitment of analytical resources and time to a further elucidation
of the health/aquatic effects observed.
D.25.3.3 Possible Sources of Chemical Toxicity—
Based on the above discussion, it appears that cadmium in the
effluent is the primary source of toxicity from Plant B126S. A
secondary source could be the concentration of ammonia which was
the highest value for the"piants surveyed.
D.25-8
-------
D.26 PLANT B143S: TITANIUM DIOXIDE PIGMENT PRODUCTION
D.26.1 Process Description
Plant B143S manufactures titanium dioxide pigments (SIC 2816)
using chloride and sulfate hydrolysis precipitation manufacturing
processes. In the chloride process, titanium ore is reacted with
chlorine to form liquid titanium tetrachloride, which is dis-
tilled and reacted with oxygen to produce titanium dioxide pre-
cipitate and chlorine. Chlorine is recycled to the process.
Impurities are removed at each step.
In the sulfate process, titanium ore is reacted with sulfuric
acid and dissolved in water to remove insoluble impurities. It
is then diluted with additional water and heated to form a tita-
nium dioxide hydrate precipitate which is filtered, cleaned and
finished. In general, the sulfate process produces several times
as much wastewater as the chloride process.
Water use at the site is shown in Figure D.26-1. Wastewater
originates from the production processes as well as runoff and
sanitary wastes from 650 people. Sulfuric acid from the sulfate
plant is neutralized with calcium carbonate and the resulting
calcium sulfate is removed by filtration. Hydrochloric acid is
neutralized with alkali, and suspended solids are settled by
lagooning. Acid spills are controlled by the plant using a
holding pond until the acid is neutralized. Treatment of the
chlorine process wastewater takes approximately 2 to 3 hours,
while sulfate plant waste treatment takes 2 days. The total
discharge averages 20,800 m3/d (5.5 MGD).
D.26-1
-------
TABLE D.26-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B143S, CORRECTED FOR BACKGROUND
Retention
time , min
13.9
25.6
6.8
10.5
15.1
17.1
27.5
30.1
28.3
32-33
C
Compound
Internal standards
Bromochloromethane
1 , 4-Dichlorobutane
Organics detected
Dichlorodi fluorome thane
Methylene chloride
Chloroform
1 , 1 , 1-Trichloroethane
Toluene
Ethylbenzene
Chlorobenzene
Xylenes
Total concentration6
:oncentration,
pg/L
400
400
<10d
25
<10d
<10d
<10d
-------
TABLE D.26-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B143S
p
to
Ul
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeablea .
% Extractable0
% Chromatographable d
% GRAV Chromatographable
Concentration in each fraction, mg/L
Ex tractable
Base/
Acid neutral Total
15
0.25 0.25 0.50
0.07 ND 0.07
0.05 0.01 0.06
<0.7
3.8
0.9
12.0
a°' of carbon tmracablc - Total mass
of purgeables _ inft
TOC K 10°
X i nn
J.UU
J T TCG •* r\ f\
TOC X 10°
of grav Chromatographable = ,,„..„ x 100
\jKAV
"ND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.26-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B143S, |jg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
3.88
322
239
128
<1.0
432,000
<2
28.8
34.5
53.1
119,000
69.6
21,400
48,000
26,600
23.9
676,000
167
<70
<40
9,110
99.2
80.2
7.95
51
Suspended
solids
NDa
241
8
2
ND
ND
ND
3.7
26.5
47.1
11,000
ND
ND
100
700
3.9
ND
29
ND
ND
90
74.2
65.9
2.01
ND
aNot detected.
D.26-6
-------
TABLE D.26-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B143S EFFLUENT
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
t , min
1'.78
2.12
2.38
2.84
3.30
3.60
4.01
4.82
5.34
5.90
6.0
7.25
8.02
10.75
34.5
Log tr
0.2504
0.3263
0.3766
0.4533
0.5185
0.5563
0.6031
0.6830
0.7275
0.7709
0.7782
0.8603
0.9042
1.0314
1.5378
Adjusted
Peak peak
Calcd. height, height,
log P cm cm
0.16
0.56
0.82
1.23
1.57
1.77
2.02
2.44
2.67
2.90
2.94
3.38
3.61
4.28
6.95
1.6
1.0
1.0
1.5
3.25
4.75
2.5
0.9
0.6
0.6
0.6
0.3
0.7
0.2
0.1
140
85
85
130
280
410
210
77
51
51
51
26
60
17
9
Estimated
. concentration
FSD , index ,
% ppb
910d
570
570
850
1,900
2,700
1,400
510
340
340
340
170
400
110
57
276
23
23
326
72C
556
55C
21
14
<1C
14
2
<1C
3e
2
Positive
or. c
negative
_
-
-
_
_
_
-
-
-
-
-
-
+
+
+ '
Retention time.
'Percent full-scale deflection.
"A positive response is defined as log P >3.5 with % FSD >25.
d ~
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.26-7. CALCULATED LC50'S FOR MYSID SHRIMP (NYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B143S IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
Hour
24
48
72
96
LC50 ,
percent effluent
58
48
42
22
95% Confidence limits
percent effluent
50-70
38-63
33-55
17-30
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal con-
centrations of test material in artifi-
cial seawater. pH and dissolved oxygen
remained within acceptable ranges through-
out the tests.
aAssay performed by EG&G Bionomics, Pensacola,
Florida.
D.26.3 Data Interpretation
D.26.3.1 Evaluation of Protocol Performance—
The effluent from plant B143S was found to be moderately toxic to
mysid shrimp (LC50 = 22%; 96 hr). The inorganic and anionic
analyses indicated a potential toxicological problem only with a
manganese level of 25.9 mg/L. Since the TOC level in the waste-
water was only 15 mg/L versus a protocol decision point of 50 mg/L,
no GC/MS analysis was conducted on the organic species. In this
case, the protocol did not develop a strong candidate for the
source of toxicity found in the wastewater.
D.26.3.2 Protocol Changes Suggested for Plant B143S—
In the case of plant B143S additional studies are required to
identify the source of wastewater toxicity with some degree of
confidence.
D.26-8
-------
D.26.3.3 Possible Sources of Chemical Toxicity—
Two potential sources of the wastewater toxicity are (1) the
manganese level of 25.9 mg/L, and (2) the presence of chlorinated
organics in the purgeable organics fraction from the wastewater.
D.26-9
-------
D.27 PLANT B147S: STAINLESS STEEL MANUFACTURE
D.27.1 Process Description
Plant B147S is a mnaufacturer and processor of stainless steel
ingots, billets, bars, rods, and wire (SIC 3312). Scrap stainless
steel and other alloying metals are melted to form ingots which
are sold or formed into billets, bars, rods, or wires.
Figure D.27-1 is a wastewater flow diagram for the plant. No
actual wastewater treatment plant exists; the plant combines
contact (process) and noncontact water before discharge. Most
of the discharge is noncontact cooling water.
PLANT
PROCESS
WASTEWATER
NON-CONTACT
COOLING
WATER
SAMPLING
POINT
DISCHARGE
Figure D.27-1.
Wastewater flow diagram for Plant B147S
stainless steel manufacturing.
D.27-1
-------
D.27.2 Analysis Results, Plant B147S
The following tables in this section present the data collected
for Plant B147S.
TABLE D.27-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT B147S
Temper- Dissolved
Flow -ature, oxygen,
m3/d (MOD) pH °C (°F) mg/L Comments
No data taken by Maryland EPA.
TABLE D.27-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT B147S
(mg/L, except as noted)
Detection
Species Concentration limit
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (pg/L)
Filtered mercury (ng/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
4
<0.020
<0.010
7.3
<0.20
<0.20
0.229
0.089
2.63
1.0
28.0
12.2
16.5
1.0
0.020
0.010
0.1
0.20
0.20
NR*
NR
0.04
0.25
0.5
1.0
2.5
aNo detection limit reported.
D.27-2
-------
TABLE D.27-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
B147S, CORRECTED FOR BACKGROUND
Retention Concentration,
time, min Compound ng/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
10.5 Methylene chloride <10
15.1 Chloroform 12,
17.9 Dichlorobromomethane <10
Total concentration6 <32
,aBackground correction based on average of organic
free water blanks.
Internal standards added to sample immediately
before purging.
cQuantitated relative to area of major ion of
authentic standards.
10 pg/L is quantitation limit of computerized
identification system for this compound.
eSummation of concentrations of detected compounds,
excluding standards.
D.27-3
-------
TABLE D.27-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT B147S
N>
-J
I
Concentration in each
Extractable
Base/
Analysis Acid neutral
TOC
GRAV 0.25 3.93
TCO ND ND
TCG ND 0.53
Purgeable organics
Carbon balance
% Purgeable3 .
% Extractable
% Chromatographable ,
% GRAV Chromatographable
fraction, mg/L
Total
30
4.18
ND
0.53
<0.03
13.9
1.8
12.7
i
a0/ f r-.rhnn _nr_r-hln Total mass of purgeables '
JLx/v^
°/ of r-jar-Vinn ov-f--!--!^-*--!^! o — 7 "*" Vv"Y v inn
/o (Ji. v^ciiUUIl CAUi uC UcUJXC — ___ X J.UU
c TCO ~¥ TCG
To or carDon cnromai.ograpnaoj.e — — TOO — J.uu
^y r>f nrav r?hToma1-nrfranha'hl & — — v inn
indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.27-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT B147S, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
<3.0
107
.<80
25.2
<1.0
20,700
12.4
<6
62.5
36.4
276
<10
2,930
5,350
18.6
31.3
8,300
56.7
<70
143
53.6
<25
<5.0
<5.0
43
Suspended
solids
NDa
25
ND
ND
ND
700
ND
ND
19.8
24.2
196
ND
ND
290
4.0
ND
ND
ND
ND
103
1.5
ND
ND
ND
3.0
detected.
D.27-5
-------
TABLE D.27-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT B147S EFFLUENT
o
to
i
cr<
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
t , min
1.28
1.82
2.02
3.18
3.28
3.59
3.84
4.25
5.88
7.25
8.01
9.0
10.2
11.0
14.88
17.3
25.25
28.32
Log tr
0.1072
0.2601
0.3054
0.5024
0.5159
0.5551
0 . 5843
0.6284
0.7694
0.8603
0.9036
0.9542
1.0086
1.0414
1.1726
1.2380
1.4023
1.4521
Adjusted
Peak peak
Calcd. height, height,
log P cm cm
-0.60
0.21
0.45
1.49
1.56
1.77
1.92
2.15
2.90
3.38
3.60
3.87
4.16
4.33
5.02
5.37
6.23
6.50
0.4
2.2
0.8
1.8
1.8
4.4
4.1
0.8
2.9
0.3
3.3
0.3
0.2
0.35
0.2
0.1
0.15
0.1
34
190
68
150
150
380
350
68
250
26
280
26
17
30
17
9
13
9
Estimated
. concentration
FSD , index ,
% ppb
230d
1,300
460
1,000
1,000
2,500
2,300
460
1,700
170
1,900
170
110
200
170
60
90
60
9
50
18
41
39C
45e
93
18
<16
2e
<16
7
5
8
7
2
3
2
Positive
negative0
_
-
-
_
_
-
•-
-
-
-
•f
+
+
.+
+
+
+
+
Retention time.
Percent full-scale deflection.
«»
"A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.27-7. CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE B147S IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER
LC50,
Hour percent effluent
24
48
72
96
>100
>100
>100
54
95% Confidence limits
percent effluent
b
"•
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal con-
centrations of test material in artifi-
cial seawater. pH and dissolved oxygen
remained within acceptable ranges
throughout the tests.
aAssay performed by EG&G Bionomics, Pensacola,
Florida.
Confidence limits not calculated due to LC50
£100%.
cConfidence unit not calculated.
D.27.3 Data Interpretation
D.27.3.1 Evaluation of Protocol Performance—
The effluent from plant B147S was found to be moderately toxic to
mysid shrimp (LC50 = 54%; 96 hr). From the inorganic and anionic
analyses, the only observed potential source of the toxicity is
cadmium at 13.6 pg/L, with perhaps some contribution from low
levels of chromium and copper (42.7 yg/L and 12.2 pg/L, respec-
tively). Since the toxicity was below the 50% LC50 decision point
and exhibited a TOC of only 20 mg/L, the only further analysis
conducted was to test for potential bioaccumulative species.
These data indicated 8 peaks above log P <3.5, but all the peaks
were estimated to be of relatively low concentration.
D.27-7
-------
In this case, the protocol performance is marginal in yielding
the information required to unambigiously rationalize the toxi-
city exhibited by the effluent.
D.27.3.2 Protocol Changes Suggested for Plant B147S—
To fully assess the protocol efficiency in a case like plant B147S
where marginal toxicity is observed, it is important to retest
toxicity of the effluent after the suspected source of toxicity
has been removed (cadmium, chromium, and copper in the case of
plant B148S). If indeed the toxicity was significantly reduced,
the protocol would be supported.
D.27.3.3 Potential Sources of Chemical Toxicity—
The only chemical sources identified which could explain the
moderate toxicity of plant B147S effluent are the cadmium concen-
tration of 13.6 |jg/L with perhaps some syneigistic contribution
from moderately high concentrations of chromium and copper.
D.27-8
-------
D.28 PLANT C169S: INDUSTRIAL INORGANIC CHEMICALS PRODUCTION
D.28.1 Process Description
Plant C169S is an industrial inorganic chemicals production plant
(SIC 2819). Chromic acid, sodium bisulfate, potassium bichro-
mate, potassium chromate, sodium chromate tetrahydrate, and
ammonium bichromate are the major products from this site.
Figure D.28-1 is a water flow diagram for the plant process water
and noncontact cooling water.
0.144 MGD
1
RIVER
9285 MGD
r
.285 MGD
PROCESS
A
1 {
I
1.285 MGD 0.144 MGD
DIS. 002 DIS.0010255MGD
8.00 MGD
SAMPLING
POINT <
8.00
DIS
f
PROCESS
B
EVAPORATION
0.220 MGD "
PROCESS
CONSUMPTION
0.015 MGD "
) J
0.020 MGD
SOLID WASTE
MGD
.002
MUNICIPAL WATER SUPPLY 0.580 MGD
r
0.151 MGD 0.030 MGD
! ' \ '
UTILITY SANITARY
1 \
0.151 MGD 0.030 MGD
DIS. 001 MUNICIPAL SEWAGE
Figure D.28-1.
Water flow diagram for Plant C169S: indus-
trial inorganic chemicals production.
D.28-1
-------
D.28.2 Analysis Results, Plant C169S
The following tables in this section present the data collected
for Plant C169S.
TABLE D.28-1 SUMMARY OF SAMPLING CREW TESTS AND
OBSERVATIONS FOR PLANT C169S
Temper- Dissolved
Flow ature, oxygen,
m3/d (MGD) pH °C (°F) mg/L Comments
No data taken by Maryland EPA.
aData not available,- no reading taken.
TABLE D.28-2 WET CHEMICAL, ION CHROMATOGRAPHY,
AND NPDES RESULTS FOR PLANT C169S
(mg/L, except as noted)
Species
Total organic carbon
Total cyanide
Total phenol
Total suspended solids
Total mercury (Mg/L)
Filtered mercury (pg/L)
Total Kjeldahl nitrogen
Ammonia nitrogen
Nitrate plus nitrite
nitrogen
Fluoride
Chloride
Sulfate
Sulfite
Dissolved phosphate (ortho)
Concentration
5
<0.020
<0.010
416
<0.20
<0.20
0.443
0.163
0.607
<2.5
7,600
454
<250
0.040
Detection
limit
1.0
0.020
0.010
0.1
0.20
0.20
NRa
NR .
0.04
2.5
0.5
1.0
250
NR
aNo detection limit reported.
D.28-2
-------
TABLE D.28-3 PURGEABLE ORGANIC COMPOUNDS IN EFFLUENT
C169S, CORRECTED FOR BACKGROUND
RetentionConcentration,
time, min Compound pg/L
Internal standards
13.9 Bromochloromethane 400
25.6 1,4-Dichlorobutane 400
Organics detected0
6.8 Dichlorodifluoromethane <^®
15.1 Chloroform
20.6 > Trichloroethylene
-------
TABLE D.28-4 ORGANIC CARBON DISTRIBUTION FOR EFFLUENT C169S
N)
o>
Concentration
in each
fraction, mg/L
Extractable
Analysis
TOC
GRAV
TCO
TCG
Purgeable organics
Carbon balance
% Purgeablea .
% Extractable0
% Chromatographable ,
% GRAV Chromatographable
Acid
0.25
ND
0.08
Base/
neutral
0.98
ND
0.22
Total
5
1.23
ND
0.30
<0.04
0.8
24.6
6.0
24.4
*% of carbon purgeable = Total mass of^purgeables
b% of carbon extractable = TC°TQCGRAV x 100
c TCO 4- TCG
% of carbon Chromatographable = „.„„ x 100
100
of grav Chromatographable =
TCG
TOC
x 100
"ND indicates total concentration is less than 0.01 mg/L.
-------
TABLE D.28-5. ICAP METALS ANALYZED BY EPA
REGION V, PLANT C169S, pg/L
Compound
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Li
K
Mg
Mn
Mo
Na
Ni
Pb
Sn
Sr
Ti
V
Y
Zn
Unfiltered
effluent
<15
<400
1,460
31
<5
137,000
<10
<30
529
<30
<400
65.3
122,000
386,000
159
79.6
2,020,000
<75
<350
<200
2,510
<75
.<25
<25
<200
Suspended
solids
NDa
ND
ND
ND
ND
ND
ND
ND
174
ND
ND
ND
ND
ND
ND
29.6
ND
ND
ND
ND
ND
ND
ND
ND
ND
detected.
D.28-5
-------
TABLE D.28-6.
BIOACCUMULATION POTENTIAL TEST RESULTS,
PLANT C169S EFFLUENT
P
N)
I
Peak
number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
t , min Log t
1.33
1.80
2.25
2.80
3.29
3.60
3.94
4.29
4.62
5.37
5.90
7.1
8.02
10.42
20.88
34.60
0.1239
0.2553
0.3522
0.4472
0.5172
0.5563
0.5955
0.6325
0.6646
0.7300
0.7709
0.8513
0.9042
1.0179
1.3197
1.5391
Calcd.
log P
-0.51
0.18
0.69
1.20
1.57
1.77
1.98
2.17
2.34
2.69
2.90
3.33
3.61
4.21
5.80
6.96
Peak
height,
cm
0.5
2.3
0.45
2.0
3.1
5.3
0.9
0.9
0.7
0.5
5.1
0.4
5.0
0.25
0.1
0.1
Adjusted
peak
height,
cm
43
200
38
170
260
450
77
77
60
43
440
34
430
21
9
9
Estimated
. concentration
FSD , index ,
% ppb
290d
1,300
260
1,100
1,800
3,000
510
510
400
290
2,900
230
2,800
140
60
60
11
52
10
446
69e
656
196
21
16
11
ioe
4
<1C
4
2
2
Positive
or
negative
»
-
-
_
_
_
-
-
-
-
-
-
+
+
+
+
Retention time.
Percent full-scale deflection.
c
A positive response is defined as log P >3.5 with % FSD >25.
See Appendix C for an explanation on FSD in excess of 100.
Corrected for background interference.
-------
TABLE D.28-7,
.CALCULATED LC50'S FOR MYSID SHRIMP (MYSIDOPSIS
BAHIA) EXPOSED TO EFFLUENT SAMPLE C169S IN
STATIC, UNAERATED, ARTIFICIAL SEAWATER3
LC50 ,
Hour percent effluent
24
48
72
96
>100
>100
48
12
95% Confidence limits,
percent effluent
_b
-
32-80
9-16
Note: Salinity was 25 o/oo and temperature was
22°C. Values were based on nominal con-
centrations of test material in artifi-
cial seawater. pH and dissolved oxygen
remained within acceptable ranges
throughout the tests.
*Assay performed by EG&G Bionomics, Pensacola,
Florida.
^Confidence limits not calculated due to LC50
D.28.3 Data Interpretation
D. 2 8. 3.1 Evaluation of Protocol Performance —
The wastewater effluent from plant C169S was moderately to highly
toxic to mysid shrimp (LC50 = 12%, 96 hr). The results of the
inorganic and ionic analyses point only to the chromium concentra-
tion of 355 M9/L as a potential source of the toxicity. Since
the effluent TOC was only 5 mg/L (well below the 50 mg/L protocol
decision point), no GC/MS analyses were conducted on the wastewater.
The only other sources of toxicity noted were the fact that the
purgeables (<40 pg/L) were all chlorinated aliphatic compounds.
Mysid shrimp are known to be particularly sensitive to chlori-
nated organic compounds. Only one of the four bioaccumulative
peaks (log P = 3.61) was at a significant concentration and this
peak was not identified.
D.28-7
-------
In the case of Plant C169S it is concluded that the analytical
protocol most probably did not succeed in identifying the source
of toxicity.
D.28.3.2 Protocol Changes Suggested for Plant C169S—
Although the protocol did not perform well in identifying the
source of toxicity in the plant C169S wastewater effluent, no
changes in the protocol which would pinpoint the cause for the
toxicity are evident at this time. In this case, frequent moni-
toring and flowthrough bioassay should be undertaken to help
identify the source of toxicity.
D.28.3.3 Potential Sources of Chemical Toxicity—
The only potentially toxic substances observed by the protocol
were chromium at 355 ng/L and possibly chlorinated aliphatic
organic compounds in the purgeable fraction at <40 |jg/L.
D.28-8
-------
D.29 REFERENCES
1. Hausch, C. Computerized Printout of Log P Values by Increas-
ing Molecular Carbon Content and Increasing Log P, 1980.
D.29-1
-------
Section
APPENDIX E
PHASE III PLANT-BY-PLANT ANALYSIS
RESULTS FOR SEDIMENTS
Plant
Paqe
E.I
E.2
E.3
E.4
E.5
E.6
E.7
E.8
E.9
E.10
E.ll
E.12
E.13
E.14
E.15
E.16
E.17
E.18
E.19
E.20
E.21
E.22
A109: Organic and Inorganic Chemical Manufacture
B112D: Wood Treating and Preserving
B119D: Plastics Manufacture. ....
C150D: Municipal Sewage Treatment Plant
C156D: Municipal Sewage Treatment Plant
C157D: Nylon Resins and Fibers Manufacture . . .
C161D: Municipal Sewage Treatment Plant. ....
C164D: Combined Municipal/Industrial Sewage
Treatment Plant
B141S: Combined Municipal/Industrial Sewage
Treatment Plant
B142S: Industrial Waste Treatment and Recovery
Plant . .
A101: Synthetic Organic Fibers Manufacture .
B113D: Nylon Manufacture
B124D: Tobacco Processing
C151D: Commercial Electric Power Generation. . .
C153D: Commercial Fertilizer Production
C154D: Ammoniated Fertilizer Production
C158D: Combined Municipal/Industrial Sewage
Treatment Plant
C159D: Pork Slaughter, Processing, and Packaging
C160D: Rubber Product Manufacture
B126S: Inorganic Pigment Manufacture
B143S: Titanium Dioxide Pigment Production . . .
C169S: Agricultural Chemicals Production ....
E-2
E-7
E-12
E-16
E-20
E-24
E-28
E-32
E-36
E-41
E-46
E-50
E-55
E-59
E-63
E-68
E-73
E-77
E-82
E-86
E-90
E-95
E-l
-------
TABLE E.l-1. ORGANIC COMPOUNDS IN SEDIMENT A109, CORRECTED FOR BACKGROUND
a,b
M
i
RRI
201.88
_j
-
-
298.82
397.66
498.50
105.65
130.53
132.31
157.35
160.90
168.98
181.10
182.39
Peak
No.
13
1
2
3
23
26
29
4
5
6
7
8
9
10
11
Retention
time,
min
21.00
6.50
7.60
14.67
25.12
28.93
32.97
15.08
16.62
16.73
18.28
18.50
19.00
19.75
19.83
i
Compound
Internal standard
Anthracene-d10
Recovery standard 'e
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '"'
n-C14-alkane
n-Cjg-alkane
Unknown hydrocarbon
(71, 85, 57, 212)
Unknown phthalate
Unknown (82, 81, 95, 97)
Unknown (71, 57, 85, 69)
Unknown (71, 85, 57)
Unknown (71, 57, 85)
Peak area,
total counts
319,727
66,391
140,737
241,710
344,672
219,116
226,398
9,197
4,075
27,726
22,373
2,575
3,962
6,905
4,766
Amount
detected,
pg/L
33.3
29.0
19.8
26.8
28.6
27.7
19.4
1.0
0.4
2.9
2.3
0.3
0.4
0.7
0.5
Amount
spiked, Recovery,
|jg/L percent
-
35.6 81.5
35.9 55.2
33.5 80.0
34.3 83.5
33.7 82.2
34.0 57.2
(continued)
-------
TABLE E.l-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
pg/L
Amount
spiked,
Recovery,
percent
199.35 12
20.88
W
i
to
204.94
207.76
236.00
242.35
256.47
277.18
278.82
284.24
294.12
334.55
380.00
419.95
454.36
541.19
551.26
555.38
559.95
14
15
16
17
18
19
20
21
22
24
25
27
28
30
31
32
33
21.13
21.25
22.45
22.72
23.32
24.20
24.27
24.50
24.92
26.50
28.25
29.82
31.20
34.83
35.27
35.45
35.65
Organics detected (continued)
f.g.h
Phenanthrene and/or benzene,
l,l'-(l,2-ethynediyl)bis and/or
2-Cyclopropen-l-one,
2,3-diphenyl
Unknown (71, 85, 57)
Unknown (71, 85, 57)
Unknown (71, 85, 57)
4H-cyclopenta[d,e,f]phenanthrene
plus 50% unknown hydrocarbon
Unknown phthalate
Sulfur, mol. (S8)
Unknown (206, 95, 81, 109)
Fluoranthene
Unknown (216, 71, 85, 57)
Unknown (97, 215, 216, 83)
Unknown (71, 97, 85, 57, 216)
Unknown phthalate
Unknown (85, 71, 57, 97, 207)
Unknown (207, 191, 95, 81)
Silicon-containing Unknown
(207, 85, 71, 57)
Silicon-containing Unknown
(207, 95, 81, 215, 233)
Silicon-containing Unknown (81,
207, 95, 108, 161, 231, 215)
10,298
4,223
5,895
4,555
4,931
3,960
45,311
3,448
15,397
7,373
4,223
226
5,393
2,249
3,338
2,427
1,312
17,508
1.1
0.4
0.6
0.5
0.5
0.4
4.7
0.4
1.6
0.8
0.4
0.03
0.6
0.2
0.3
0.3
0.1
1.8
(continued)
-------
TABLE E.l-1 (continued)
RRI
566.82
Peak
No.
34
35
Retention
time,
min
35.95
37.63
Compound
Organics detected (continued) '"'
Silicon-containing Unknown
(207, 191, 95)
Unknown (124, 95, 109)
Total concentration
Amount Amount
Peak area, detected, spiked, Recovery,
total counts jjg/L pg/L percent
4,454 0.5
5,712 0.6
24.4
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.03 |jg/g relative to the
anthracene-d1() internal standard.
CAnthracene-d1() internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
eQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
^"Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
\eterition time greater than benzo(g,h,i)perylene.
-------
TABLE E.l-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT A109
Parameter
% Mgisturea
TCO , mg/g (ppt)
GRAV, mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
73.8
0.01
1.0
0.25
0.02
Carbon balance
% Extractabled 1.01 x 10'1
% Chromatographable f 2.6 x 10~2
% Extractables identified 2.0
-Reported as 1 .[Freeze-Dry Wtj x
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.l-1.
*% of carbon extractable = TC°aGRAV * 100
e% of carbon Chromatographable = TC01^aTCG x 100
% extractables identified
.Identified organics detected v -, nn
TCO + GRAV x xuu
E-5
-------
TABLE E.l-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT A109
Concentration3 ,
Metal
Ag <22
Al 42,600
B 78
Ba 210
Be 0.88
Ca 4,080
Cd 5.9
Co 27
Cr 84
Cu 74
Fe 34,600
Mg 10,800
Mn 350
Mo <25
Na 41,200
Ni 120
P 610
Pb <35
Sb <54
Si 850
Sr 74
Ti 1,160
V 120
Zn 450
Original sample solids
content was 26.2%.
E-6
-------
TABLE E.2-1. ORGANIC COMPOUNDS IN SEDIMENT B112D, CORRECTED FOR BACKGROUNDS,b
M
RRI
202.35
1 -J
-
-
299.76
397.14
497.51
153.31
199.35
Peak
No.
5
la
Ib
2
12
22
29
3
4
Retention
time,
min
21
6
7
14
25
28
.02
.42
.55
.65
.17
.92
Compound
Internal standard
Anthracene-dio
Recovery standard 'e
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
32.95 Perylene-d12
.18
20
.03
.88
Organics detectedf 'g'h
9H-Fluorene
Phenanthrene and/or Benzene,
Peak area,
total counts
229
5
11
44
175
62
52
12
,085
,646
,447
,861
,227
,279
,743
,009
Amount
detected,
pg/L
667
20
10
29
34
30
30
35
.0
.1
.6
.5
.5
.2
.6
.0
Amount
spiked,
pg/L
-
35
35
33
34
33
34
.6
.9
.5
.3
.7
.0
Recovery,
percent
-
56.5
29.5
88.0
100.5
89.7
90.0
l,l'-(l,2-ethynediyl)bis and/or
2-Cyclopropen-l-one,
2,3-diphenyl 24,274 70.7
218.31 6 21.70 1-Naphthaleneacetonitrile and/or
9H-Carbazole and/or 1-Naphthyl
isocyanide, 2-methyl / 15,117 44.0
225.35 7 22.0 Dihydropyrene (isomer) l 9,176 26.7
242.49 8 22.73 Cyclopenta[d,e,f]phenanthrene 34,381 100.1
258.92 9 23.43 9,10-Anthracenedione plus
20% unknown 18,752 54.6
284.74 10 24.53 Fluoranthene 177,023 515.4
298.59 11 25.12 Pyrene 93,248 271.5
(continued)
-------
TABLE E.2-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
Amount
spiked,
Recovery,
percent
301.82 13
395.84 21
399.22 23
404.23
428.61
24
25
473.13 26a
474.38 26b
25.25
w
1
00
311.69
320.78
329.09
334.29
336.88
338.96
376.62
380.26
14
15
16
17a
17b
18
19
20
25.63
25.98
26.30
26.50
26.60
26.68
28.13
28.27
28.87
29.00
29.20
30.18
31.97
32.02
Organics detected (continued)
f,g,h
203)
1 , 4-Ethenoanthracene , 1 , 4-dihydro
plus 50% unknown (218, 189)
Unknown (218, 189, 219)
Pyrene, methyl (isomer)
Pyrene, methyl (isomer) and/or
HH-Benzo [b] f luorene
1 IH-Benzo [b ] f luorene
HH-Benzo[b] f luorene
Unknown (218, 232, 202,
Benzo[b]naphthothiophene (isomer)
Benzo [g,h,i]fluoranthene plus
30% compounds listed for #21
Naphthacene and/ or Benz[ a] anthracene
and/or Chrysene and/or Triphenylene
and/ or Benzo[c]phenanthrene
Naphthacene and/ or Benz [a] anthracene
and/or Chrysene and/or Triphenylene
and/or Benzo[c]phenanthrene
Unknown (228. 227, 226)
Benz [a] anthracene, methyl and/or
Chrysene, methyl
Benzo pyrene (isomer) and/or
Benz[e]acephenanthrylene and/or
Perylene and/or Benzo fluoran-
thene (isomer)
Benzo pyrene (isomer) and/or
Benz[e]acephenanthrylene and/ or
Perylene and/ or Benzo fluoran-
thene (isomer)
176,951
15,436
10,350
62,109
47,749
14,205
14,444
8,548
20,878
31,072
65,001
19,684
9,972
51,796
34,881
515.2
44.9
30.1
180.8
139.0
41.4
42.1
24.9
60.8
90.5
189.3
57.3
29.0
150.8
101.6
(continued)
-------
TABLE E.2-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
Ug/L
Amount
spiked,
Recovery,
percent
490.05 27 32.65
493.04 28 32.77
Organics detected (continued)
f,g,h
Benzo pyrene (isomer) and/or
Benz[e]acephenanthrylene and/or
Perylene and/or Benzo fluoran-
thene (isomer)
Benzo pyrene (isomer) and/or
Benz[e]acephenanthrylene and/or
Perylene and/or Benzo fluoran-
thene (isomer)
Total concentration
34,203
99.6
31,331 91.2
3,006.5
W
I
vO
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 24.9 |jg/g relative to the
anthracene-d10 internal standard.
Anthracene-djo internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 667.0 M9/9 in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
i.
j
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE E.2-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B112D
Parameter
% Moisture*
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
76.2
0.68
4.9
8.01
3.01
Carbon balance
% Extractabled 5.58 x 10'1
% Chromatographable f 8.69 x 10'1
% Extractables identified 53.9
Deported as 1 .[Freaze-Dry WtJ x 1QO
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.2-1.
*% of carbon extractable = TCOGRAV x 100
e% of carbon Chromatographable = TC°10'3'rCG x 100
% extractables identified
Identified organics detected inn
TCO + GRAV X •"•"
E-10
-------
TABLE E.2-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B112D
Concentration3,
Metal |jg/g
Ag <22
Al 42,100
B 73
Ba 230
Be 1.2
Ca 4,430
Cd 5.7
Co 27
Cr 110
Cu 220
Fe 35,500
Mg 10,400
Mn 260
Mo <25
Na 42,900
Ni 78
P 1,280
Pb <35
Sb <53
Si 790
Sr 100
Ti 1,200
V 140
Zn 540
aOriginal sample solids
content was 23.8%.
E-ll
-------
TABLE E.3-1. ORGANIC COMPOUNDS IN SEDIMENT B119D, CORRECTED FOR BACKGROUND
a,b
M
M
KJ
RRI
201.88
J
-
-
298.82
397.66
498.50
—
-
199.35
217.65
225.41
230.82
246.12
253.18
261.88
289.65
294.12
Peak
No.
7
1
2
5
16
18
20
3
4
6
8
9
10
11
12
13
14
15
Retention
time,
min
21.0
6.48
7.58
14.67
25.12
28.93
32.97
10.58
13.88
20.88
21.67
22.0
22.23
22.88
23.18
23.55
24.73
24.92
Compound
£
Internal standard
Anthracene-d10
d e
Recovery standard '
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '"'•
Cyclopentasiloxane , decamethyl
Unknown (73, 341, 147, 429)
Unknown (190, 178, 220)
Unknown (95, 82, 123, 68)
Unknown (82, 81, 123, 95)
Unknown (81, 82, 95, 123)
Unknown (109, 233, 95, 123)
Unknown (233, 123, 109, 95)
Unknown (109, 95, 81, 191, 262)
Benzene, l,l'-cyclohexylidenebis
S-containing unknown (216, 217,
108)
Peak area,
total counts
303,599
55,756
135,737
227,440
350,085
243,018
177,046
4,519
3,336
2,092
6,565
5,067
6,626
7,998
5,310
13,879
3,893
3,376
Amount
detected,
pq/L
33.3
26.3
20.4
27.3
32.4
33.8
32.4
1.5
1.1
0.7
0.7
0.6
0.7
0.9
0.6
1.5
0.4
0.4
Amount
spiked,
pg/L
-
35.6
35.9
33.5
34.3
33.7
34.0
0.5
0.4
0.2
Recovery,
percent
-
74.0
56.7
81.5
94.5
100.2
95.2
(continued)
-------
TABLE E.3-1 (continued)
W
i
M
U)
RRI
331.17
454.36
550.80
Peak
No.
17
19
21
Retention
time,
min
26.37
31.20
35.25
Compound
Organics detected (continued) 'g/
Phenanthrene , tetramethyl (isomer)
Unknown (71, 85, 57, 207)
Unknown (85, 71, 207, 57, 281)
Total concentration
Peak area,
total counts
2,797
2,794
5,727
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
0.3
0.3
0.6
8.1
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.2 |jg/g relative to the
anthracene-d10 internal standard.
°Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
oeuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction. "
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d1(), internal
standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE E.3-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B119D
Parameter
% Moisture*
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
49.8
ND'
0.48
0.07
0.01
Carbon balance
% Extractabled 4.8 x 10~2
% Chromatographable f 7 x 10~3
% Extractables identified 2.1
Deported as 1 - x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
°From Table E.3-1.
rt Tr*n •+• PPAV
°% of carbon extractable = IQ^ x 100
e% of carbon Chromatographable = =-^3 x 100
% extractables identified
_ Identified organics detected
TCO + GRAV x
%D indicates total concentration is less than
0.01 mg/g.
E-14
-------
TABLE E.3-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B119D
Concentration3,
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
25,600
25
230
0.74
3,690
3.0
24
52
25
27,400
3,670
680
<25
25,400
53
820
<34
<53
270
58
1,170
94
110
aOriginal sample solids
content was 50.2%.
E-15
-------
TABLE E.4-1. ORGANIC COMPOUNDS IN SEDIMENT C150D, CORRECTED FOR BACKGROUNDa,b
W
H
o>
Retention
RRI
201.88
_J
-
-
298.82
397.66
498.50
Peak
No.
9
1
2
3
11
12
14
time,
min
21.00
6.42
7.53
14.67
25.12
28.93
32.97
Amount
Peak area, detected,
Compound
c
Internal standard
Anthracene-d10
Recovery standard /e
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
total counts
324,972
19,614
28,614
207,442
325,052
221,741
257,025
pg/L
33.3
12.6
4.3
22.8
25.9
24.6
16.7
Amount
spiked, Recovery,
pg/L percent
-
35.6 35.5
35.9 12.0
33.5 68.0
34.3 75.5
33.7 73.0
34.0 49.0
Orqanics detected 'g>
105.65
132.31
157.35
180.78
199.35
204.94
397.40
4
5
6
7
8
10
13
15.08
16.73
18.28
19.73
20.88
21.13
28.92
n-C14-alkane
n-C15-alkane
n-C16-alkane
n-C17-alkane
Benzene , 1 , 1 ' - (1 , 2-ethylnediyl)bis
and/ or Phenanthrene
n-Hydrocarbon (71, 85, 57)
Unknown phthalate (149, 167, 150)
20,902
74,500
36,698
12,276
2,445
4,939
9,824
2.1
7.6
3.8
1.3
0.3
0.5
1.0
•
(continued)
-------
TABLE E.4-1 (continued)
M
i
Retention
Peak time ,
RRI No. min Compound
Total concentration
Amount
Peak area, detected,
total counts M<3/L
16.6
Amount
spiked,
Mg/L
Recovery,
percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.3 pg/g relative to the
anthracene-d10 internal standard.
Q
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE E.4-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C150D
Parameter
% Moisture3
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Carbon balance
% Extractabled
% Chromatographable ~
% Extractables identified
Value
69.2
0.04
0.84
0.35
0.02
8.8 x 10~2
3.9 x 10~2
2.3
a- _„, ne , rFreeze-Dry Wtl v _nft
" I Wet Wt I •*.«'»'
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.4-1.
of carbon extractable = TC011:3GRAV x
100
% of carbon Chromatographable = 103 x 100
ables identified
Identified organics detected
% extractables identified
TCO + GRAV x 10°
E-18
-------
TABLE E.4-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C150D
Metal
Concentration,a'
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
45,100
74
220
0.91
6,060
6.0
28
87
78
39,300
10,300
350
<25
37,600
79
610
<34
<53
590
78
1,180
120
500
aOriginal sample solids
content was 30.8%.
Average of duplicate
analyses.
E-19
-------
TABLE E.5-1. ORGANIC COMPOUNDS IN SEDIMENT C156D, CORRECTED FOR BACKGROUND
a,b
w
1
K)
O
Peak
RRI No.
202.35 8
-k 1,2
3,4,
5
6,7
299.77 9
399.22 10
499.50 12
Retention
time,
min
21.02
e.55^
7.65D
14.683
25.17
29.00
33.03
Compound
c
Internal standard
Anthracene-d10
Recovery standard '
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-dio
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf /g/h
Peak area,
total counts
293,014
117,901
207,039
368,614
756,243
560,644
655,307
Amount
detected,
pg/L
66.7
27.9
20.3
25.3
36.2
40.1
51.3
Amount
spiked,
Mg/L
-
35.6
35.9
33.5
34.3
33.7
34.0
Recovery,
percent
-
78.5
56.5
75.5
105.5
119.0
151.0
419.65 11
29.82 Unknown phthalate (149, 167,
150)
10,591
2.4
(continued)
-------
TABLE E.5-1 (continued)
w
i
N)
Retention
Peak time,
RRI No. min Compound
Total concentration1
Amount
Peak area, detected,
total counts ug/L
2.4
Amount
spiked,
pq/L
Recovery,
percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 2.4 pg/g relative to the
anthracene-d10 internal standard.
c
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 66.7 M9/9 in original sample.
i)euterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'splitting of phenol-d5, dichlorobenzene-d4 and biphenyl-d10 peaks due to acetonitrile/methylene
chloride mixed solvents. The retention time listed is that for the major peak.
Detention time less than biphenyl.
-------
TABLE E.5-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C156D
Parameter
% Moisture3
TCO , mg/g (ppt)
GRAY , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
31.2
ND1
0.27
0.03
0.002
Carbon balance
% Extractabled 2.7 x 10~2
% Chromatographable f 3.0 x 10~3
% Extractables identified 74
Reported as 1 -[Free^D% "J x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.5-1.
*% of carbon extractable = TC°1Q3GRAV x 100
e% of carbon Chromatographable = TCO + TCG x 100
ia
% extractables identified
_ Identified organics detected
TCO + GRAV X
indicates total concentration is less than
0.01 mg/g.
E-22
-------
TABLE E.5-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C156D
Metal
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
Concentration, a '
ng/g
<22
6,830
23
28
<0.39
5,630
0.89
7.0
16
11
7,050
1,230
82
<25
20,600
19
310
<35
<54
1,400
32
490
37
45
aOriginal sample solids
content was 68.8%.
Average of duplicate
analyses.
E-23
-------
TABLE E.6-1. ORGANIC COMPOUNDS IN SEDIMENT C157D, CORRECTED FOR BACKGROUND
a,b
w
K)
RR1
201.88
_J
-
-
298.82
397.66
498.50
105.65
106.62
132.31
157.35
181.10
256.71
276.71
294.12
331.17
406.23
Peak
No.
9
1
2
3
13
15
19
4
5
6
7
8
10
11
12
14
16
Retention
time,
min
21.00
6.48
7.58
14.67
25.12
28.93
32.97
15.08
15.14
16.73
18.28
19.75
23.33
24.18
24.92
26.37
29.27
Compound
Internal standard
Anthracene-djo
Recovery standard '
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-dj2
Organics detected '"'
n-C14-alkane
Benzene, l,l'-oxybis-
n-C15-alkane
n-C16-alkane
n-Hydrocarbon (71, 85, 57)
Unknown phthalate (149, 150)
Sulfur, mol. (S8)
Unknown (216, 217, 170)
Phenanthrene, tetramethyl (isomer)
and/or phenanthrene, l-methyl-7-
(1-methylethyl)
Unknown (71, 85, 57)
Peak area, d
total counts
294,296
54,277
125,684
208,098
254,428
191,968
166,563
3,124
3,018
13,307
6,192
3,297
3,464
3,193
3,328
3,199
2,953
Amount
e tec ted,
pg/L
33.3
26.2
19.6
25.8
24.0
27.3
30.8
•
0.4
0.3
1.5
0.7
0.4
0.4
0.4
0.4
0.4
0.3
Amount
spiked, Recovery,
ug/L percent
-
35.6 73.5
35.9 54.5
33.5 77.0
34.3 70.0
33.7 81.0
34.0 90.5
(continued)
-------
TABLE E.6-1 (continued)
Peak
RRI No.
Retention
time , •
min Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pq/L ug/L percent
Organics detected (continued) 'g'
454.36 17
486.28 18
535.24 20
550.81 21
31.20 Unknown (71, 85, 57)
32.48 Unknown (207, 97, 87, 57)
34.57 Unknown (207, 82, 95, 97)
35.25 Unknown (85, 71, 207, 57)
Total concentration
3,687 0.4
2,543 0.3
3,403 0.4
7,727 0.9
7.2
Background correction based on reagent and/or method blank analysis. Appendix J.
7 Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
** tion program in the Hewlett-Packard system and are greater than 0.3 pg/g relative to the
anthracene-d10 internal standard.
Anthracene-djo internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 ug/g in original sample.
HDeuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
eQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE E.6-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C157D
Parameter Value
% Moisture9 63.1
TCO , mg/g (ppt) ND9
GRAV, mg/g 0.56
TCG, mg/g 0.01
Organics detected by GC/MS , mg/g 0.02
Carbon balance
% Extractable 5.6 x 10~2
% Chromatographable f 1.2 x 10~2
% Extractables identified 1.8
Sported as 1 - x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.6-1.
of carbon extractable = a x 100
e TCO + TCG
% of carbon Chromatographable = 1Q3 — — x 100
% extractables identified
_ Identified organics detected , nn
TCO + GRAV
"ND indicates total concentration is less than
0.01 mg/g.
E-26
-------
TABLE E.6-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C157D
Concentration3 ,
Metal
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<21
47,000
28
380
1.6
4,530
5.3
36
85
53
42,200
5,340
1,310
<24
17,900
83
1,140
<34
<52
220
76
1,130
120
200
aOriginal sample solids
content was 36.9%.
E-27
-------
TABLE E.7-1. ORGANIC COMPOUNDS IN SEDIMENT C161D, CORRECTED FOR BACKGROUND
a,b
w
K>
00
RRI
201.88
_j
-
-
299.77
399.22
499.50
199.68
250.00
255.87
276.53
284.51
294.13
419.65
554.79
560.50
Peak
No.
5
1
2
3
11
12
14
4
6
7
8
9
10
13
15
16
Retention
time,
min
21.00
6.55
7.65
14.68
25.17
29.00
33.03
20.90
23.05
23.30
24.18
24.52
24.93
29.82
35.45
35.70
Compound
Internal standard
Anthracene-d10
Recovery standard 'e
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-dxo
Pyrene-d10
Chrysene-d12
Perylene-d12
Orqanics detectedf 'g'h
Phenanthrene
Unknown (69, 97, 83)
Unknown (73, 129, 97, 213, 256)
Sulfur, mol. (S8)
Fluoranthene
Unknown (212, 216)
Unknown phthalate (149, 167,
150)
Unknown (95, 215, 81, 108, 149,
233, 207)
Unknown (95, 81, 231)
Peak area.
total counts
278,504
87,463
183,022
356,397
762,760
610,810
697,064
494
11,200
29,259
27,696
16,405
13,874
34,476
1,626
41,498
Amount
detected,
MQ/L
66.7
27.8
19.7
31.7
36.0
38.1
47.9
0.1
2.7
7.0
6.6
3.9
3.3
8.3
0.4
9.9
Amount
spiked, Recovery,
pg/L percent
-
35.6 78.2
35.9 55.0
33.5 94.5
34.3 105
33.7 113
34.0 141
(continued)
-------
TABLE E.7-1 (continued)
w
i
N>
vO
RRI
567R35
Peak
No.
17
18
Retention
time ,
min Compound
Organics detected (continued) '"'
36.00 Unknown (95, 191, 81)
37.67 Unknown (124, 95, 81)
Total concentration
Peak area,
total counts
33,066
28,163
Amount Amount
detected, spiked, Recovery,
|jg/L pg/L percent
7.9
6.7
56.8
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.1 pg/g relative to the
anthracene-d10 internal standard.
Q
Anthracene-d10 internal standard added immediately prior to analysis,- 50 mg/L injected equivalent
to 66.7 pg/g in original sample.
i)euterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
6Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
"""Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
Retention time greater than benzo(g,h,i)perylene.
-------
TABLE E.7-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C161D
Parameter Value
% Moisture3 60.4
TCO, mg/g (ppt) 0.01
GRAY, mg/g . 0.77
TCG, mg/g 0.27
Organics detected by GC/MS , mg/g 0.06
Carbon balance
% Extractabled 7.8 x 10~2
% Chromatographable f 2.8 x 10~2
% Extractables identified 7.7
Reported as 1 -[Free^^ Wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.7-1.
A Tpn •+• riPAv
°% of carbon extractable = igS x 100
e% of carbon Chromatographable = TC01^3TCG x 100
IQ3
_ Identified organics detected
% extractables identified
TCO + GRAV X
E-30
-------
TABLE E.7-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C161D
Concentration3,
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
10,900
22
66
<0
2,950
1
8
27
31
40,200
2,840
78
<25
20,700
32
490
<35
<54
2,800
44
640
60
97
.40
.3
.7
aOriginal sample solids
content was 39.6%.
E-31
-------
TABLE E.8-1. ORGANIC COMPOUNDS IN SEDIMENT C164D, CORRECTED FOR BACKGROUND
a,b
w
I
U)
to
RRI
201.88
_J
-
-
299.06
397.66
498.00
293.65
,
305.97
454.36
550.80
Peak
No.
4
1
2
3
6
8
10
5
7
9
11
Retention
time,
min
21.00
6.47
7.57
14.67
25.13
28.93
32.95
24.90
25.40
31.20
35.25
i
Compound
Internal standard
Anthracene-d10
Recovery standard 'e
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-dl2
Organics detected '"'
S-containing unknown (216, 217,
108)
Unknown (237, 195, 252)
Unknown (71, 85, 57, 97, 207)
Unknown (207, 85, 71, 57, 281)
Peak area,
total counts
373,370
76,736
151,998
257,814
380,026
232,318
212,496
3,874
11,273
3,920
3,516
Amount Amount
detected, spiked,
jjg/L ug/L
33.3
27.8 35.6
18.8 35.9
25.3 33.5
28.8 34.3
27.1 33.7
25.6 34.0
0.3
1.0
0.3
0.3
Recovery,
percent
-
78.0
52.5
75.5
84.0
80.5
75.2
(continued)
-------
TABLE E.8-1 (continued)
w
i
U>
Retention
Peak time ,
RRI No. min Compound
Total concentration
Amount
Peak area, detected,
total counts pg/L
1.9
Amount
spiked,
uq/L
Recovery,
percent
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.3 pg/g relative to the
anthracene-d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-djo, internal
standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
-------
TABLE E.8-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C164D
Parameter
% Moisture*
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
56.8
NDg
0.46
0.08
0.002
Carbon balance
% Extractabled 4.6 x 10~2
% Chromatographable f 8 x 10"3
% Extractables identified 0.4
"Reported as 1 -[F""£;D% Wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
GFrom Table E.8-1.
*% of carbon extractable =-TC°1QaGRAV x 100
e TCO + TCG
% of carbon Chromatographable = 1Qa x 100
% extractables identified
_ Identified organics detected
TCO + GRAV x
^ND indicates total concentration is less than
0.01 mg/g.
E-34
-------
TABLE E.8-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C164D
Concentration3 ,
Metal
Ag <22
Al 45,100
B 44
Ba 240
Be 1.4
Ca 2,700
Cd 4.8
Co 31
Cr 76
Cu 48
Fe 40,500
Mg 6,730
Mn 850
Mo <25
Na 24,600
Ni 73
P 630
Pb <35
Sb <54
Si 450
Sr 72
Ti 1,070
V 120
Zn 200
aOriginal sample solids
content was 43.2%.
E-35
-------
TABLE E.9-1. ORGANIC COMPOUNDS IN SEDIMENT B141S, CORRECTED FOR BACKGROUND
a,b
M
u>
cr>
RRI
201.88
J
-
-
298.82
397.66
498.75
105.65
132.31
142.33
144.43
149.11
158.00
165.75
166.56
168.66
173.83
Peak
No.
19
la
Ib
2
33
34
35
3
4
5
6
7
8
9
10
11
12
Retention
time,
min
21.00
6.4
7.57
14.67
25.12
28.93
32.98
15.08
16.73
17.35
17.48
17.77
18.32
18.80
18.85
18.98
19.30
i
Compound
Internal standard
Anthracene-d10
Recovery standard 'e/
Phenol-ds
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected* 'g'h
n-C14-alkane
n-C^-alkane
Benzene, (C10H21-) (isomer)
Benzene, (C10H2i-) (isomer)
Benzene, (C10H21-) (isomer)
Unknown phthalate (149, 177,
105, 150)
Benzene, (C11H23-) (isomer)
Benzene, (C11H23-) (isomer)
Benzene, (C11H23-) (isomer)
Benzene, (C11H23-) (isomer)
Peak area,
total counts
271,157
8,846
139,886
252,317
328,076
257,281
308,904
35,279
67,091
62,288
63,634
56,159
426,291
51,706
143,119
123,767
97,690
Amount
detected,
(jg/L
33.3
6.0
19.4
24.2
26.4
27.6
38.6
4.3
8.2
7.6
7.8
6.9
52.4
6.3
17.6
15.2
12.0
Amount
spiked. Recovery,
pg/L percent
-
35.6 16.9
35.9 54.0
33.5 72.2
34.3 77.0
33.7 81.8
34.0 113.6
(continued)
-------
TABLE E.9-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
pq/L
Amount
spiked,
Recovery,
percent
Organics'detected (continued)
f,g,h
181.10
182.39
188.69
189.66
192.08
196.93
208.24
214.82
217.18
220.71
228.24
240.24
244.71
246 . 59
247.76
251.76
259.06
264.71
270.82
289.65
551.49
560.64
13
14
15
16
17
18
20
21
22
23
24
25
26a
26b
27
28
29
30
31
32
36
37
19.75
19.83
20.22
20.28
20.43
20.73
21.27
21.55
21.65
21.80
22.12
22.63
22.82
22.9
22.95
23.12
23.43
23.67
23.93
24.73
35.28
35.68
n-C17-a!
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Benzene
Unknown
Benzene
Unknown
Unknown
Unknown
(1-me thyldecy1)
(C12H25-) (isomer)
(C12H2g-) (isomer)
(Ci2H25-) (isomer)
(Ci2H25) (isomer)
(1-methylundecyl)
(C13H27-) (isomer)
(C13H27-) (isomer)
(C13H27-) (isomer)
(CiaH27-) (isomer)
(1-me thyIdodecy1)
(C14H29-) (isomer)
(C14H29-) (isomer)
(C14H29-) (isomer)
(Cj4H2g—) (isomer)
(Ci4H29-) (isomer)
[145, 159, 117, 242)
(1 methyl tridecyl-)
36,171
156,736
134,859
123,812
97,752
98,633
122,994
126,310
81,492
76,875
70,209
84,989
35,369
21,156
33,842
37,154
39,704
24,114
28,430
19,195
4,388
43,498
4.4
19.2
16.6
15.2
12.0
12.1
15.1
15.5
10.0
9.4
8.6
10.4
4.3
2.6
4.2
4.6
4.9
3.0
3.5
2.4
0.5
5.3
(continued)
-------
TABLE E.9-1 (continued)
w
u>
oo
Retention
Peak time ,
RRI No. min Compound
Total concentration
Amount
Peak area, detected.
total counts pg/L
322.1
Amount
spiked,
ug/L
Recovery,
percent
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 4% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.5 pg/g relative to the
anthracene-dio internal standard.
c
Anthracene-djo internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 Mg/g i-n original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d1o, internal
standard.
^Tentative identification, not confirmed with authentic standards.
Tflajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
1Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE E.9-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B141S
Parameter
% Mgisturea
TCO , mg/g (ppt)
GRAV, mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Carbon balance
% Extractable ,
% Chromatographable f
% Extractables identified
Value
73.4
0.20
1.70
1.18
0.32
1.9 x 10"1
1.4 x 10-1
16.8
ar . d , [Freeze-Dry Wfl
Reported ac 1 ^ Wet Wt J x
100
,.4-^x3 » A. /"i
instead of C7 to eliminate hexane solvent.
GFrom Table E.9-1.
^ of carbon extractable = TC°1QaGRAV x 100
e% of carbon Chromatographable = TC°1Qa'L'rv' x 100
% extractables identified
Identified organics detected v inn
TCO + GRAV x
E-39
-------
TABLE E.9-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B141S
Concentration3 ,
Metal _ pg/g _
Ag
Al 28,000
B 67
Ba 210
Be 0.82
Ca 2,320
Cd 4.6
Co 26
Cr 250
Cu 150
Fe 27,400
Mg 5,730
Mn 480
Mo <24
Na 26,600
Ni 100
P 1,370
Pb <34
Sb <52
Si 4,180
Sr ^ 65
Ti 940
V 110
Zn 360
aOriginal sample solids
content was 26.6%.
E-40
-------
TABLE E.10-1. ORGANIC COMPOUNDS IN SEDIMENT B141S, CORRECTED FOR BACKGROUND
a,b
RRI
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts ug/L ug/L percent
W
i
Internal standard
201.88 13,
14,15
21
Anthracene-djo
291,316
33.3 100
_k
-
-
298.82
398.18
498.75
—
-
105.65
106.46
130.53
132.31
157.35
181.10
1
2
5,6
23,24
29
32
3
4
7
8
9
10
11
12a
6.50
7.60
14.67
25.12
28.95
32.98
13.15
13.47
15.08
15.13
16.62
16.73
18.28
19.75
Recovery standard '
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '*'
Naphthalene, methyl (isomer)
Naphthalene, methyl (isomer)
n-C14-alkane
Naphthalene, 2-butyl plus
10% unknown
Cl-containing unknown (192,
194, 128)
n-C15-alkane
n-C16-alkane
n-Ci7-alkane
(
63,643
128,659
197,485
321,914
259,205
307,887
23,079
12,559
36,370
33,531
12,455
97,839
55,145
29,435
24.0
16.8
21.8
29.9
33.2
40.3
2.6
1.4
4.2
3.8
1.4
11.2
6.3
3.4
35.6 67.5
35.9 46.8
33.5 65.2
34.3 87.3
33.7 98.5
34.0 118.6
• „
(continued)
-------
TABLE E.10-1 (continued.)
RRI
182.39
207.76
236.00
240.00
270.59
W 277.18
*• 284.71
10 288.84
302.60
340.26
355.33
357.40
371.69
411.47
419.95
567.28
Peak
No.
12b
16
17
18
19
20
21
22
25
26
27a
27b
28
30
31
33
Retention
time ,
min Compound
19.83
21.25
22.45
22.62
23.92
24.20
24.52
24.70
25.27
26.72
27.30
27.38
27.93
29.48
29.82
35.97
Organics detected (continued) /g'
n-C17-alkane
Benzene, 1,3,3-trimethylnonyl)
Cl-containing unknown (85, 71,
57, 256)
Benzene, (1-methyldodecyl)
Benzene, (C14H29~) (isomer)
Sulfur, mol. (S8)
Fluorene
Cls-biphenyl (isomer)}
Cl5-biphenyl (isomer).
Cl6-biphenyl (isomer)}
Cl6-biphenyl (isomer)}
Cl5-biphenyl (isomer)}
Cl6-biphenyl (isomer)
Cl7-biphenyl (isomer)
Unknown phthalate (149, 167.
150)
Unknown (191, 95, 81, 69)
Amount Amount
Peak area, detected, spiked. Recovery,
total counts pq/L uq/L percent
22,563
21,773
11,899
12,144
9,949
75,365
18,623
16,064
17,026
8,413
26,843
17,650
20,993
12,390
35,653
20,796
2.6
2.5
1.4
1.4
1.1
8.6
2.1
1.8
1.9
1.0
3.1
2.0
2.4
1.4
4.1 •
2.4
(continued)
-------
TABLE E.10-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Amount Amount
Peak area, detected, spiked, Recovery,
total counts MQ/L pg/L percent
Total concentration
70.7
W
w
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 4% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 1.0 pg/g relative to the
anthracene-d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 |jg/g in original sample.
oeuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration,based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Between 4 and 8 isomer of Cl6-biphenyl and Cl5-biphenyl were detected at levels below 3 pg7g-
k
Retention time less than biphenyl.
-------
TABLE E.10-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B142S
Parameter
% Moisture*
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Carbon balance
% Extractabled
% Chromatographable f
% Extractables identified
Value
67.6
0.12
2.40
1.20
0.07
2.52 x 10"1
1.32 x 10"1
2.8
Reported as 1 -[Free^D^ Wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.10-1.
of carbon extractable = x 10o
s TCO •+• TCG
% of carbon Chromatographable = vQ3 x 100
% extractables identified
Identified organics detected
TCO + GRAV x
E-44
-------
TABLE E.10-3 SEDIMENT ICAP METALS ANALYSIS,
RESULTS, PLANT B142S
Metal
Concentr ationa ,
Ag <22
Al 36,200
B 47
Ba 190
Be <0.39
Ca 5,380
Cd 11
Co 49
Cr 590
Cu 300
Fe 51,000
Mg 7,350
Mn 390
Mo <25
Na 32,400
Ni 550
P 2,430
Pb 160
Sb <53
Si 3,810
Sr 88
Ti 2,250
V 210
Zn 570
aOriginal sample solids
content was 32.4%.
E-45
-------
TABLE E.ll-1. ORGANIC COMPOUNDS IN SEDIMENT A101, CORRECTED FOR BACKGROUND
a,b
w
RRI
201.88
_j
-
-
298.82
397.66
498.00
180.78
184.81
199.35
230.82
276.47
294.12
388.31
406.23
454.36
550.80
Peak
No.
7
1
2
3
11
13
16
4
5
6
8
9
10
12
14
15
17
Retention
time.
min
21.0
6.47
7.57
14.67
25.12
28.93
32.95
19.73
19.98
20.88
22.23
24.17
24.92
28.57
29.27
31.20
35.25
Compound
Internal standard
Anthracene-d10
Recovery standard '
Phenol-ds
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-djo
Chrysene-d12
Perylene-d12
Organics detectedf 'g'h
Hydrocarbon (71, 85, 57)
Hexathiepane
Unknown (178, 190, 220, 160)
Unknown (81, 95, 82, 97)
Sulfur, mol. (S8)
Unknown (216, 217)
Unknown (81, 96, 95, 82)
Unknown (85, 71, 57)
Unknown (85, 71, 57)
Silicon-containing unknown
(85, 71, 57, 207)
Peak area,
total counts
313,151
55,573
98,125
222,821
328,731
191,018
159,678
2,495
3,920
2,177
3,121
3,256
3,850
3,374
2,562
5,388
8,103
Amount
detected,
Mg/L
33.3
25.6
14.6
26.0
29.4
26.7
27.5
0.3
0.4
0.2
0.3
0.3
0.4
0.4
0.3
0.6
0.9
Amount
spiked. Recovery,
pg/L percent
-
35.6 72.0
35.9 40.8
33.5 77.5
34.3 85.8
33.7 79.2
34.0 81.0
(continued)
-------
TABLE E.I1-1 (continued)
Retention Amount Amount
Peak time, Peak area, detected, spiked, Recovery,
RRI No. min Compound total counts pg/L |jg/L percent
Organics detected (continued) '"'
578.95 18 36.48 Unknown (207, 206, 95) 11,207 1.2
Total concentration1 5.3
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.2 pg/g relative to the
anthracene-djo internal standard.
cAnthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
£
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE E.ll-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT A101
Parameter
% Moisture3
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
74.6
ND
0.40
0.19
0.005
Carbon balance
% Extractable 4.0 x 10~2
% Chromatographable f 1.9 x 10"2
% Extractables identified 1.25
s rTi*T^^ f^ n r± ^T^T^\r \A?^ I
Reported as 1 - y^tWt x 10°
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.ll-1.
*% of carbon extractable = TC01^3GRAV x 100
% of carbon Chromatographable = •= — 1Q3 x 100
% extractables identified
_ Identified organics detected
TCO + GRAV x
indicates total concentration is less than
0.01 mg/g.
E-48
-------
TABLE E.ll-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT A101
Concentration3,
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
51,700
70
240
2.1
2,080
5.2
29
93
58
40,200
7,760
240
<25
35,900
74
670
<35
<54
410
69
920
140
2,090
aOriginal sample solids
content was 25.4%.
E-49
-------
TABLE E.12-1. ORGANIC COMPOUNDS IN SEDIMENT B113D, CORRECTED FOR BACKGROUND
a,b
w
i
in
o
RRI
202.35
_j
-
-
298.82
397.66
498.50
105.65
130.70
132.31
157.35
181.10
199.35
256.71
276.71
Peak
No.
10
1
2
3
15
16
22
4
5
6
7
8
9
,11
12
Retention
time,
min
21 .02
6.47
7.57
14.64
25.12
28.93
32.97
15.08
16.63
16.73
18.28
19.75
20.88
23.33
24.18
Compound
£
Internal standard
•Anthracene-d10
Recovery standard '
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf 'g'h
n-C14-alkane
Cl-containing unknown (192, 194,
64, 128, 130)
n-Cjs-alkane
n-C^-alkane
n-Hydrocarbon (74, 85, 57)
Penanthrene and/or benzene.
l,l'-(l,2-ethynediyl)bis- plus
unknown
Unknown phthalate (149, 150, 205)
Sulfur, mol. (S8)
Peak area.
total counts
328,055
59,484
120,928
233,708
320,223
182,440
184,228
6,332
3,703
22 , 147
11,602
3,562
5,717
3,560
41,442
Amount
detected,
|jg/L
33.3
27.1
17.2
26.4
27.3
23.8
26.4
0.6
0.4
2.2
1.2
0.4
0.6
0.4
4.2
Amount
spiked, Recovery,
ug/L percent
-
35.6 76.0
35.9 48.0
33.5 78.7
34.3 79.5
33.7 70.7
34.0 77.7
(continued)
-------
TABLE E.12-1 (continued)
M
in
RRI
284.71
294.12
361.82
419. .95
443.64
454.36
486.28
500.00
534.78
550.80
Peak
No.
13
14
17
18
19
20
21
23
24
25
26
Retention
time,
tnin
24.52
24.92
27.55
29.82
30.77
31.20
32.48
33.03
34.55
35.25
37.47
Compound
Organics detected (continued) 'g'
Fluoranthene
Unknown (216, 217, 170, 108)
Unknown (71, 85, 57, 97)
Unknown phthalate (149, 166, 150)
Unknown (218, 274, 219, 202)
Unknown (71, 85, 57, 99)
Unknown (82, 96, 97, 207)
Unknown (85, 71, 57, 264)
Unknown (82, 96, 57, 207, 69)
Unknown (85, 71, 207, 99)
Unknown (82, 96, 97, 207, 57)
Total concentration
Peak area,
total counts
4,633
4,424
2,665
2,619
8,150
7,547
23,929
20,140
12,908
15,905
Amount Amount
detected, spiked, Recovery,
pg/L MQ/L percent
0.5
0.4
0.3
0.3
0.8
0.8
2.4
2.0
1.3
1.6
20.4
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.3 pg/g relative to the
anthracene-d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
(continued)
-------
TABLE E.12-1 (continued)
CQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
ilajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^ ^Retention time less than biphenyl.
ts> k_
Retention time greater than benzo(g,h,i)perylene.
-------
TABLE E.12-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B113D
Parameter
% Moisture*
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
43.8
NDg
0.56
0.11
0.02
Carbon balance
% Extractabled 5.6 x lO^2
% Chromatographable f 1.1 x 10~2
% Extractables identified 3.5
a r IT fa A <7 A ^T) T* TT W"t~ H
Reported as 1 "|FreeS^DS WrJ x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
GFrom Table E.12-1.
*% of carbon extractable = TC°1QaGRAV x 100
e TCO •+• TCG
% of carbon Chromatographable = 1Qa x 100
% extractables identified
_ Identified organics detected , OQ
TCO + GRAV
%D indicates total concentratio is less than
0.01 mg/g.
E-53
-------
TABLE E.12-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B113D
Concentration,a'
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
24,400
16
240
<0.39
5,210
3.4
24
49
32
29,800
4,490
510
<25
25,200
51
830
<34
<53
520
75
1,450
64
150
aOriginal sample solids
content was 56.2%.
Average of duplicate
analyses.
E-54
-------
TABLE E.13-1. ORGANIC COMPOUNDS IN SEDIMENT B124D, CORRECTED FOR BACKGROUND
a,b
W
I
yi
in
RRI
201.88
_i
-
1
298.82
397. 66 '
498.50
105.65
132.31
157.35
181.10
230.82
294.12
406.23
443.14
454.36
Peak
No.
8
1
2
3
11
12
17
4
5
6
7
9
10
13
14
15
Retention
time,
min
21.00
6.48
7.58
14.67
25.12
28.93
32.97
15.08
16.73
18.28
19.75
22.23
24.92
29.27
30.75
31.20
Compound
Internal standard
Anthracene-djo
Recovery standard 'e
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-djo
Pyrene-d10
Chryene-d12
Perylene-d12
Organics detected 'g/
n-C14-alkane
n-C15-alkane
n-C16-alkane plus 10% unknown
compound
n-C17-alkane
Unknown (81, 95, 82, 123)
Unknown (216, 170, 217, 218)
n-Hydrocarbon (71, 85, 57,
99, 97, 240)
Unknown (218, 274, 202, 219)
Unknown (95, 71, 57, 99, 207)
Peak area,
total counts
335,262
58,116
101,779
220,168
333,895
188,894
162,069
4,849
17,861
9,229
6,130
2,664
333,895
3,116
3,327
6,458
Amount
detected,
pq/L
33.3
24.2
15.4
24.1
28.0
24.7
26.9
0.5
1.8
0.9
0.6
0.3
33.2
0.3
0.3
0.6
Amount
spiked, Recovery,
pg/L percent
-
35.6 68.0
35.9 42.8
33.5 72.0
34.3 81.7
33.7 73.2
34.0 79.0
•••-
(continued)
-------
TABLE E.13-1 (continued)
w
in
Peak
RRI No.
486.04 16
534.78 18
562.24 19
]r
- 20
Retention
time ,
min Compound
Organics detected (continued) 'g'
32.47 Unknown (82, 97, 96, 83, 207, 57)
34.55 Unknown (82, 96, 97, 207, 81, 83)
35.75 Silicon-containing unknown (71,
85, 57, 207, 96)
37.47 Silicon-containing unknown (207,
96, 82, 57)
Total concentration
Amount Amount
Peak area, detected, spiked, Recovery,
total counts pg/L M9/L percent
4,093 0.4
7,165 0.7
10,399 1.0
1,061 0.1
40.7
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.1 pg/g relative to the
anthracene-dio internal standard.
CAnthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 ug/g in original sample.
oeuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
CQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
Retention time greater than benzo(g,h,i)perylene.
-------
TABLE E.13-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B124D
Parameter
% Moisture*
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organ! cs detected by GC/MS , mg/g
Value
60.6
NDg
0.48
0.14
0.041
Carbon balance
% Extractable 4.8 x 10"2
% Chromatographable f 1.4 x 10~2
% Extractables identified 8.5
Reported as 1 -[Free^;D^ Wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.13-1.
*% of carbon extractable = TCO^GRAV x 100
e TCO + TCG
% of carbon Chromatographable = 3 - x 100
% extractables identified
1Q3
Identified organics detected
TCO + GRAV x
indicates total concentration is less than
0.01 mg/g.
E-57
-------
TABLE E.13-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B124D
Concentration3,
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
38,400
31
320
0.66
4,050
4.8
31
70
42
36,200
4,840
990
<25
21,400
71
810
<35
<53
1,090
68
1,310
100
150
Original sample solids
content was 39.4%.
E-58
-------
TABLE E..14-1. ORGANIC COMPOUNDS IN SEDIMENT C151D, CORRECTED FOR BACKGROUND
a,b
W
01
vo
RRI
201
298
397
498
.41
.82
.66
.00
105.65
132.31
157.19
180.78
204.94
Peak
No.
8
1
2
1 3
10
11
12
4
5
6
7
9
Retention
time,
min
20
6
7
14
25
28
32
15
16
18
19
. 21
.98
.42
.55
.67
.12
.93
.95
.08
.73
.27
.73
.13
Compound
Internal standard0
Anthracene-d10
Recovery standard '
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf 'g'h
n-C14-alkane
n-Cig-alkane
n-Cje-alkane
n-C17-alkane
Unknown (85, 71, 57)
Total concentration1
Peak
total
316
37
73
215
321
236
225
26
88
40
14
5
area,
counts
,644
,898
,471
,251
,455
,487
,570
,203
,168
,351
,837
,759
Amount
detected,
|jg/L
33.3
17.3
10.3
23.6
26.4
28.2
27.9
2.8
9.3
4.2
1.6
0.6
18.5
Amount
spiked,
Mg/L
35.6
35.9
33.5
34.3
33.7
34.0
Recovery,
percent
48.5
28.8
70.5
77.0
83.8
82.0
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.6 pg/g relative to the
anthracene-d,0 internal standard.
(continued)
-------
TABLE E.14-1 (continued)
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 M9/9 in original sample.
ceuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-dio. internal
standard.
"Tentative identification, not confirmed with authentic.standards.
M h
• najor masses of unknown compounds are listed in parentheses according to decreasing intensity.
o Possible molecular ions are underlined.
1Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE E.14-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C151D
Parameter
% Moisture*
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g '
Organics detected by GC/MS , mg/g
Carbon balance
% Extractabled
% Chromatographable ,.
% Extractables identified
Value
46.3
0.02
0.38
0.08
0.02
4 x 10~2
1 x 10'1
5.0
aT»n™r.i-nrf «« 1 . P1^6626-1^ Wt 1 v 1 rtrt
Wet Wt J
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.14-1.
% of carbon extractable = 1Qa x 100
& TCO + TCG
% of carbon Chromatographable = 10a x 10°
% extractables identified
Identified organics detected
TCO + GRAV
E-61
-------
TABLE E.14-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C151D
Concentration3,
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn '
<22
23,000
16
210
<0.40
4,070
3.2
23
46
38
24,100
3,300
470
<25
23,400
84
610
<35
<53
1,500
66
1,340
67
140
Original sample solids
content was 58.7%.
E-62
-------
TABLE E.15-1. ORGANIC COMPOUNDS IN SEDIMENT C153D, CORRECTED FOR BACKGROUND
a,b
w
o<
CO
RRI
201.88
_j
-
-
-
299.30
299.76
398.70
499.50
105.98
128.27
132.63
157.35
181.10
199.68
242.49
258.92
I
Peak
No.
10
la
1
2,3
16,17
22
26
4
5
6
7
8
9
11
12
Detention
time,
min
21.00
6.47
7.62
14.58,
14.70
25.15,
25.17
28.98
33.03
15.10
16.48
16.75
18.28
19.75
20.90
22.73
23.43
Compound
Internal standard
Anthracene-d10
Recovery standard '
Phenol-ds
1 , 2-Dichlorobenzene-d4
Biphenyl-diQ
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '"'
n-C14-alkane
Acenaphthalene , 1 , 2-dihydro
n-C15-alkane
n-CiQ-alkane
n-C17-alkane
Penanthrene and/or Benzene,
1 , 1 ' - (1 , 2-ethynediyl)bis
4H-cyclopenta [d , e , f ] phenanthrene
Unknown (204, 208, 202, 203, 180)
Peak area.
total counts
250,204
4,261
298,973
412,552
606,956
318,457
474,800
17,059
13,698
50,721
33,203
18,472
73,658
19,962
18,444
Amount
detected.
pg/L
66.7
2.0
35.1
38.2
34.6
31.1
43.4
4.5
3.7
13.5
8.9
4.9
19.6
5.3
4.9
Amount
spiked. Recovery,
|jg/L percent
-
35.6 5.5
35.9 97.7
33.5 114.0
34.3. 101.0
33.7 92.2
34.0 127.5
(continued)
-------
TABLE E.15-1 (continued)
w
I
RRI
277.00
284.74
294.13
301.82
329.09
334.29
380.26
473.38
490.55
493.78
576.48
Peak
No.
13
14
15
18
19
20
21
23
24
25
27
Retention
time,
min
24.20
24.53
24.93
25.25
26.30
26.50
28.27
31.98
32.67
32.80
36.40
Compound
Organics detected (continued) '"'
Sulfur, mol. (S8)
Fluoranthene
Cl-containing unknown (216,
212, 218)
Unknown (218, 204, 203, 202)
Pyrene, methyl (isomer) and/or
HH-Benzo fluorene (isomer)
Pyrene, methyl (isomer) and/or
HH-Benzo fluorene (isomer)
Benzo [ g , h , i ] f luoranthene
Benzo pyrene (isomer) and/or
Benz [e] acephenanthry lene and/or
Perylene and/ or Benzo f luoran-
thene (isomer)
Benz [ e ] acephenanthry lene and/ or
Benzo pyrene (isomer) and/ or
Perylene and/ or Benzo f luoran-
thene (isomer)
Benz [ e ] acephenanthry lene and/ or
Benzo pyrene (isomer) and/or
Perylene and/or Benzo fluoran-
thene (isomer)
Benzo[g,h,i]perylene and/or
Indeno[l,2,3-cd]pyrene
Peak area,
total counts
46,478
133,401
6,936
20,529
44,564
16,743
19,275
116,958
39,865
38,298
21,449
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
12.4
35.6
1.8
5.5
11.9
4.5
5.1
31.2
10.6
10.2
5.7
(continued)
-------
TABLE E.15-1 (continued)
W
Retention
Peak time ,
RRI No . min
Compound
Total concentration
Amount
Peak area, detected,
total counts pg/L
199.8
Amount
spiked,
pg/L
Recovery,
percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 1.8 pg/g relative to the
anthracene-d10 internal standard.
Anthracene-dto internal standard added immediately prior to analysis,- 50 mg/L injected equivalent
to 66.7 pg/g in original sample.
t)euterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
^lajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•^Retention time less than biphenyl.
-------
TABLE E.15-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C153D
Parameter Value
% Moisture3 73.7
TCO, mg/g (ppt) 0.04
GRAV, mg/g 0.63
TCG, mg/g 0.32
Organics detected by GC/MS , mg/g 0.20
Carbon balance
% Extractable 6.7 x 10^2
% Chromatographable ,. 3.6 x 10~2
% Extractables identified 29.9
•Reported as 1 -
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.15-1.
of carbon extractable = TCO^GRAV x 100
e% of carbon Chromatographable = TC°1Q3TCG x 100
% extractables identified
_ Identified organics detected ino
TCO + GRAV
E-66
-------
TABLE E.15-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C153D
Concentration3,
Metal Mg/g
Ag <22
Al 30,000
B 60
Ba 170
Be <0.39
Ca 7,150
Cd 4.1
Co 21
Cr 73
Cu 94
Fe 27,700
Mg 7,910
Mn 250
Mo . <24
Na 42,500
Ni 55
P 2,150
Pb <34
Sb <53
Si 560
Sr 100
Ti 1,350
V 110
Zn 340
aOriginal sample solids
content was 26.3%.
E-67
-------
TABLE E.16-1. ORGANIC COMPOUNDS IN SEDIMENT C154D, CORRECTED FOR BACKGROUND
a,b
M
i
o>
00
RRI
202.35
_j
-
-
299.30
397.92
498.76
105.98
132.31
157.67
181.42
199.68
208.45
242.96
257.04
284.74
294.60
419.90
Peak
No.
9
1
2
3
15
16
21
4
5
6
7
8
10
11
12
13
14
17
Retention
time,
min
21.02
6.42
7.55
14.67
25.15
28.95
33.00
15.10
16.73
18.30
19.77
20.90
21.28
22.75
23.35
24.53
24.95
29.83
Compound
Internal standard
Anthracene-d10
Recovery standard '
Phenol-ds
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detectedf 'g'h
n-C14-alkane
n-C15-alkane
Unknown (71, 85, 57)
Unknown (71, 85, 57, 97)
Phenanthrene plus 20% unknown
Unknown (71, 85, 97, 109, 57)
Unknown (97, 95, 133, 108)
Unknown phthalate (149, 95, 97)
Fluoranthene
Unknown (212, 97, 85, 95, 216)
Unknown phthalate (149, 167,
71, 69)
Peak area,
total counts
246,162
17,535
23,375
187,814
267,042
159,094
168,822
9,663
29,398
19,205
10,504
17,107
9,099
12,654
11,722
31,978
11,802
13,172
Amount
detected,
pg/L
133.4
5.2
15.9
10.4
5.7
9.3
4.9
6.9
6.4
17.3
6.4
7.1
Amount
spiked, Recovery,
ug/L percent
-
35.6 36.1
35.9 14.6
33.5 89.8
34.3 83.9
33.7 96.2
34.0 101.4
(continued)
-------
TABLE E.16-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount
detected,
ug/L
Amount
spiked,
Recovery,
percent
473.38 18
W
o>
\D
490.80 19
493.78 20
541.78
566.90
22
23
Oganics detected (continued) '
31.98 Benzo pyrene (isomer) and/or
Benzo [ e ] acephenanthrylene
and/or perylene and/or benzo
fluoranthene (isomer) plus
20% unknown
32.68 Benzo pyrene (isomer) and/or
Benzo [ e ] acephenanthrylene
and/or perylene and/or benzo
fluoranthene (isomer) plus
20% unknown
32.80 Benzo pyrene (isomer) and/or
Benzo [ e ] acephenanthrylene
and/or perylene and/or benzo
fluoranthene (isomer) plus
20% unknown
34.88 Unknown (191, 95, 109, 81)
35.98 Unknown (191, 95, 109, 69)
38,668
18,975
17,115
9,583
15,201
21.0
10.3
9.3
5.2
8.2
(continued)
-------
TABLE E.16-1 (continued)
i
^j
o
Retention
Peak time ,
RRI No. min Compound
Total concentration
Amount
Peak area, detected,
total counts Mg/L
149.5
Amount
spiked. Recovery,
Mg/L percent
•
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 4.9 pg/g relative to the
anthracene-djo internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 133.4 pg/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
ilajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
1Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE E.16-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C154D
Parameter
% Moisture3
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Carbon balance
% Extractabled
% Chromatographable f
% Extractables identified
Value
76.9
0.23
3.1
1.88
0.15
3.33 x 10'1
2.11 x 10'1
4.5
a_ n ,n, ,n , TFreeze-Dry Wtl _nn
iw-^^-L. w>-v* —^ j. Wet Wt I *vw
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.16-1.
*% of carbon extractable = TC°10'aGRAV x 100
e TCO + TCG
% of carbon Chromatographable = 0 3 - x 10°
% extractables identified
30
Identified organics detected
TCO + GRAV
E-71
-------
TABLE E.16-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C154D
Metal
Concentration3,
Ag
Al 45,400
B 80
Ba 250
Be 1.4
Ca 4,520
Cd 6.3
Co 28
Cr 110
Cu 330
Fe 39,200
Mg 11,000
Mn 310
Mo <24
Na 41,500
Ni 80
P 1,170
Pb <34
Sb <53
Si 210
Sr 92
Ti 990
V 130
Zn 620
aOriginal sample solids
content was 23.1%.
E-72
-------
TABLE E.17-1. ORGANIC COMPOUNDS IN SEDIMENT C158D, CORRECTED FOR BACKGROUND
a,b
w
i
-0
RRI
201.41
_j
-
-
298.82
397.40
498.00
105.65
132.63
157.19
181.10
204.94
276.47
Peak
No.
8
1
2
3
11
12
13
4
5
6
7
9
10
Retention
time,
min
20.98
6.45
7.57
14.67
25.12
28.92
32.95
15.08
16.75
18.27
19.75
21.13
24.17
Compound
Internal standard
Anthracene-djo
Recovery standard '
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected '"'
n-C14-alkane
n-C15-alkane
n-C16-alkane
n-C17-alkane
n-C18-alkane
Sulfur, mol. (S8)
Peak area,
total counts
310,511
48,058
121,579
207,217
302,017
210,480
183,213
29,971
99,755
45,354
14,218
5,735
7,255
Amount
detected.
pg/L
33.3
20.6
16.9
22.6
25.5
26.6
29.2
3.2
10.7
4.9
1.5
0.6
0.8
Amount
spiked, Recovery,
pg/L percent
-
35.6 57.8
35.9 47.2
33.5 67.5
34.3 74.2
33.7 78.8
34.0 85.8
(continued)
-------
TABLE E.17-1 (continued)
Retention
Peak time ,
RRI No. min Compound
Total concentration
Amount
Peak area, detected,
total counts pg/L
21.7
Amount
spiked,
gg/L
Recovery,
percent
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.6 ug/g relative to the
anthracene-djo internal standard.
f»
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 gg/g in original sample.
d_
l)euterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
eQuantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
ilajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE E.17-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C158D
Parameter
% Moisturea
TCO , mg/g (ppt)
GRAY , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
26.3
0.02
0.38
0.12
0.02
Carbon balance
% Extractabled 5 x 10~2
% Chromatographable f 1.4 x 10~2
% Extractables identified 4.0
Deported as 1 -pMM] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
GFrom Table E.17-1.
of carbon extractable = TC°1Q3GRAV x 100
A
% of carbon chromatographable = 10a x 10°
% extractables identified
_ Identified organics detected
TCO + GRAY
E-75
-------
TABLE E.17-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C158D
Concentration3,
Metal |jg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
. Zn
<21
13,100
17
140
<0.39
3,900
2.3
17
95
21
17,800
2,500
252
<24
27,000
35
822
<34
<52
2,730
55
1,140
61
88
aOriginal sample solids
content was 73.7%.
E-76
-------
TABLE E.18-1. ORGANIC COMPOUNDS IN SEDIMENT C159D, CORRECTED FOR BACKGROUND
a,b
w
RRI
202.35
_j
-
-
298.83
398.44
498.76
Peak
No.
7
1
2,3
5
12
14
18
Retention
time,
min
21
6
7
14
25
28
33
.02
.57
.67
.68
.13
.97
.00
Compound
Internal standard0
Anthracene-d10
Recovery standard /e
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-djo
Pyrene-d10
Chrysene-d12
Perylene-d12
Peak area,
total counts
294
63
143
229
344
218
287
,841
,168
,949
,604
,931
,020
,574
Amount
detected,
kig/L
33
28
24
32
30
36
.3
.4
.6
.2
.5
.7
46.3
Amount
spiked,
pg/L
-
35.
35.
33.
34.
33.
34.
6
9
5
3
7
0
Recovery,
percent
-
79.7
68.6
96.0
89.9
108.9
136.3
4* rt li
Orqanics detected '"'
199.68 6
235.92 . 8
242.49 9
277.00 10
284.74 11
11.00 Azulene and/or Naphthalene and/or
bicyclo[4.4.1]undeca-l,3,5,7,9-
pentaen-11-one
20.90 Phenanthrene and/or Benzene, 1,1'•
(1,2-ethynedily)bis
22.45 Phenanthrene, methyl (isomer)
and/or Anthracene, methyl
(isomer)
22.73 Unknown (190, 189, 95)
24.20 Sulfur, mol. (S8)
24.53 Fluoranthene
7,510
50,145
5,042
12,491
24,007
60,992
0.8
5.7
0.6
1.4
2.7
6.9
(continued)
-------
TABLE E.18-1 (continued)
M
1
-•4
00
Retention
Peak time,
RRI No. min
301.82 13 25.25
473.13 15a 31.97
473.88 15b 32.0
490.55 16 32.67
493.28 17 32.78
.
Compound
Organics detected (continued) '"'
Unknown (218, 203, 204, 202)
Perylene and/ or Benz[e]acephenan-
thrylene and/ or Benzo[a]pyrene
and/ or Benzo[k]fluoranthene
Perylene and/ or Benz[e]acephenan-
thrylene and/ or Benzo[a]pyrene
and/ or Benzo [ k] f luor anthene
Perylene and/or Benz[e]acephenan-
thrylene and/or Benzo[a]pyrene
and/or Benzo [k]f luor anthene
Perylene and/or Benz[e]acephenan-
thrylene and/ or Benzo[a]pyren
and/or Benzo [k]f luor anthene
Total concentration
Amount Amount
Peak area, detected, spiked. Recovery,
total counts M9/L M9/L percent
11,389 1.3
26,174 3.0
19,674 2.2
18,870 2.1
23,362 2.6
29.3
Background correction based on reagent and/or method blank analysis, Appendix J.
(continued)
-------
TABLE E.18-1 (continued)
Includes all substances that were found at or above a 3% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.6 M9/9 relative to the
anthracene-d10 internal standard.
Q
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 |Jg/g in original sample.
oeuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
w e
' Quantitated relative to area of major ion for authentic standard.
^" £
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
tentative identification, not confirmed with authentic standards.
ilajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
t j
Summation of concentrations of detected compounds, excluding standards.
Retention time less than biphenyl.
-------
TABLE E.18-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C159D
Parameter
% Moisture3
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
57.6
0.05
1.4
0.54
0.03
Carbon balance
% Extractabled 1.45 x lO^1
% Chromatographable f 5.9 x 10~2
% Extractables identified 2.1
Deported as 1 -[Free^D^ wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.18-1.
*% of carbon extractable - TCO +aGRAV x 100
e% of carbon Chromatographable = TC°1QaTCG x 100
% extractables identified
= Identified organics detected , nn
TCO + GRAV x i
E-80
-------
TABLE E.18-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C159D
Metal
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
Concentration , a '
ug/g
<22
25,900
51
160
1.1
10,700
2.9
20
58
180
28,200
5,480
170
<25
42,800
49
750
170
<53
560
75
980
74
780
aOriginal sample solids
content was 42.4%.
Average of duplicate
analyses.
E-81
-------
TABLE E.19-1. ORGANIC COMPOUNDS IN SEDIMENT C160D, CORRECTED FOR BACKGROUND
a,b
M
0>
K)
RRI
201.88
J
-
-
298.82
397.66
498.50
199.35
217.65
225.41 '
230.82
276.71
284.71
298.12
443.64
447 . 38
457.36
468.83
Peak
No.
5
1
2
3
12
13
18
4
6
7
8
9
10
11
14
15
16
17
Retention
time,
min
21.00
6.47
7.58
14.67
25.12
28.93
32.97
20.88
21.67
22.00
22.23
24.18
24.52
24.92
30.77
30.92
31.32
31.78
Compound
Internal standard0
Anthracene-djo
Recovery standard '
Phenol-d5
1 ,2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-dja
Perylene-dj2
Organics detectedf '9/h
Unknown (190, 178, 220, 188)
Unknown (95, 68, 81, 82, 113)
Unknown (95, 81, 123, 82)
Unknown (95, 81, 82, 123)
Sulfur, mol. (S8)
Fluoranthene
Unknown (216, 212, 217)
Unknown (71, 85, 97, 57, 140)
Unknown (85, 71, 97, 57)
Unknown (71, 85, 97, 57)
Unknown (85, 71, 57, 97)
Peak area, c
total counts
310,156
50,669
105,553
198,676
328,857
244,845
255,991
2,014
4,747
3,424
5,030
4,048
5,287
4,035
8,033
4,593
13,683
23,589
Amount
ietected,
pg/L
33.3
23.1
15.6
23.5
23.0
31.0
11.3
0.2
0.5
0.4
0.5
0.4
0.6
0.4
0.9
0.5
1.5
2.5
Amount
spiked, Recovery,
pg/L percent
-
35.6 65.0
35.9 43.5
33.5 70.0
34.3 67.0
33.7 92.0
34.0 33.2
(continued)
-------
TABLE E.19-1 (continued)
M
i
oo
CO
RRI
541.65
551.26
566.36
Peak
No.
19
20
21
Retention
time,
min
34.85
35.27
35.93
Total
Compound
Organics detected (continued) '"'
Silicon-containing unknown (207,
191. 95, 109)
Unknown (207, 71, 85, 57)
Silicon-containing unknown (191,
207, 95, 81)
concentration
Peak area,
total counts
3,219
4,471
6,789
Amount Amount
detected, spiked, Recovery,
pg/L pg/L percent
0.3
0.5
0.7
9.9
Background correction based on reagent and/or method blank analysis, Appendix J.
Includes all substances that were found at or above a 1% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.2 pg/g relative to the
anthracene-d10 internal standard.
Q
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 33.3 pg/g in original sample.
l)euterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
3Retention time less than biphenyl.
-------
TABLE E.19-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C160D
Parameter
% Moisture3
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
70. 5,,
NDg
0.92
0.21
0.01
Carbon'balance
% Extractable6 9.2 x 10"2
% Chromatographable6 f 2.1 x 10~2
% Extractables identified 1.1
Reported as 1 -[Free^;D% Wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
°From Table E.19-1.
*% of carbon extractable = TC°1Q3GRAV x 100
e TCO + TCG
% of carbon Chromatographable = 1Qa x 10°
% extractables identified
- Identified organics detected ,nn
TCO + GRAV x -1"0
9ND indicates total concentration is less than
0.01 mg/g.
E-84
-------
TABLE E.19-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT C160D
Concentration3,
Metal |jg/g
Ag <22
Al 43,700
B 78
Ba 210
Be 2.4
Ca 3,450
Cd 6.2
Co 27
Cr 110
Cu 61
Fe 39,000
Mg 11,000
Mn 310
Mo <25
Na 38,400
Ni 74
P 540
Pb <35
Sb <54
Si 160
Sr 67
Ti 860
V 140
Zn 260
aOriginal sample solids
content was 29.5%.
E-85
-------
TABLE E.20-1. ORGANIC COMPOUNDS IN SEDIMENT B126S, CORRECTED FOR BACKGROUND
a,b
w
00
RRI
201.88
_j
-
-
298.35
397.40
498.50
105.49
132.31
157.19
199.35
276.71
284.25 .
542.10
567.28
Peak
No.
8
1
2
3
11
12
13
4
5
6
7
9
10
14
15
Retention
time,
min
21.00
6.45
7.57
14.65
. 25.10
28.92
32.97
15.07
16.73
18.27
20.88
24.18
24.50
34.87
35.97
i
Compound
Internal standard
Anthracene-d10
Recovery standard '
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Orqanics detected '"'
n-C14-alkane
n-C15-alkane
n-C16-alkane
Phenanthrene
Sulfur (58) plus 20% unknown
Fluoranthene
Unknown (191, 95, 81, 97)
Unknown (191, 95, 81, 69)
Peak area,
total counts
300,616
21,943
61,920
106,906
142,532
87,995
110,519
10,066
31,522
18,779
30,557
14,617
22,188
14,206
17,732
Amount
detected,
pg/L
66.6
23.1
19.2
25.1
23.7
25.9
32.4
2.2
7.0
4.2
6.8
3.2
4.9
3.1
3.9
Amount
spiked, Recovery,
pg/L percent
-
35.6 65.0
35.9 53.5
33.5 75.0
34.3 69.1
33.7 76.9
34.0 95.4
(continued)
-------
TABLE E.20-1 (continued)
RRI
Peak
No.
Retention
time,
min
Compound
Peak
total
area,
counts
Amount
detected,
pg/L
Amount
spiked,
ug/L
Recovery,
percent
Total concentration
35.3
Pi
i
oo
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 4% threshold on the BATCH.peak identifica-
tion program in the Hewlett-Packard system and are greater than 3.1 ug/g relative to the
anthracene-d10 internal standard.
£
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 66.6 ug/g in original sample.
ueuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
"Tentative identification, not confirmed with authentic standards.
Major masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE E.20-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B126S
Parameter
% Moisture3
TCOD, mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Carbon balance
% Extractabled
% Chromatographable f
% Extractables identified
Value
71.3
0.18
5.0
2.93
0.04
5.18 x 10'1
3.11 x 10"1
0.8
a^^.,, , „„ , „ fFreeze-Dry Wfl v nnn
Wet Wt
run w
instead of C7 to eliminate hexane solvent.
cFrom Table E.20-1.
_
Modified TCO in that run was started at C8
of carbon extractable = a x 100
TC°1QGRAV
% of carbon Chromatographable = TC°-,n3TCG x 100
% extractables identified
103
_ Identified organics detected inn
TCO + GRAV x xuu
E-88
-------
TABLE E.20-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B126S
Concentration,a'
Metal ng/g
Ag <22
Al 38,300
B 63
Ba 2,260
Be 0.84
Ca 5,530
Cd 29
Co 37
Cr 260
Cu 780
Fe 38,900
Mg 7,980
Mn 350
Mo <25
Na 56,500
Ni 140
P 1,970
Pb 230
Sb <54
Si 1,470
Sr 97
Ti 1,580
V 140
Zn 870
aOriginal sample solids
content was 28.7%.
Average o
analyses.
Average of duplicate
E-89
-------
TABLE E.21-1. ORGANIC COMPOUNDS IN SEDIMENT B143S, CORRECTED FOR BACKGROUND
;a,b
M
I
VO
O
RRI
202.35
_j
-
-
298.35
396.88
497.51
142.33
144.75
149.11
158.16
166.07
167.04
169.30
174.31
183.04
188.85
Peak
No.
23
la
Ib
2
40
41
42
4
5
6
8
9
10
11
13
16
17
Retention
time,
min
21.02
6.42
7.53
14.63
25.10
28.90
32.93
17.35
17.50
17.77
18.33
18.82
18.88
19.02
19.33
19.87
20.23
Compound
Q
Internal standard
Anthracene-d10
Recovery standard 'e
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10
Chrysene-d12
Perylene-d12
Organics detected* 'g'h
Benzene, (C10H2i-) (isomer)
Benzene, (C10H21-) (isomer)
Benzene, (C10H21-) (isomer)
Benzene, (1-methylnonyl)
Benzene, (C^H-ja-) (isomer)
Benzene, (CnH^-) (isomer)
Benzene, (CnH23-) (isomer)
Benzene, (CnH23-) (isomer)
Benzene, (1-methyldecyl) and/or
Benzene, (2-iodoethyl)
Benzene, (C12H25-) (isomer)
Peak -area,
total counts
283,493
8,322
27,252
47,708
78,169
47,289
50,365
338,482
319,551
326,028
501,412
389,622
569,501
535,413
606,111
725,031
525,320
Amount
detected,
pg/L
166.5
24.0
19.3
26.4
30.0
29.0
31.5
198.8
187.7
191.5
294.5
228.8
334.5
314.5
356.0
425.8
308.5
Amount
spiked, Recovery,
pg/L percent
-
35.6 67.5
35.9 53.7
33.5 78.7
34.3 87.5
33.7 86.2
34.0 92.5
(continued)
-------
TABLE E.21-1 (continued)
RRI
Retention
Peak time,
, No. min
Compound
Peak area,
total counts
Amount
detected,
ug/L
Amount
spiked,
MQ/L
Recovery,
percent
M
Organics detected (continued)
189.98 18 20.30 Benzene, (C12H25-) (isomer)
192.73 19 20.47 Benzene, (C12H25-) (isomer)
197.58 20 20.77 Benzene, (C12H25-) (isomer)
200.00 22 20.92 Unknown (145, 146, 117, 91,
230)
208.94 24 21.30 Benzene, (1-methylundecyl)
215.29 25 21.57 Benzene, (C13H27-) (isomer)
217.18 26 21.65 Benzene, (C13H27-) (isomer)
221.18 27 21.82 Benzene, (C13H27-) (isomer)
228.24 29 22.12 Benzene, (C13H27-) (isomer)
232.94 31 22.32 Naphthalene, l-heptyl-1,2,3,4-
tetrahydro-4-metyl
240.24 33 22.63 Benzene, (1-methyldodecyl)
244.71 34 22.82 Benzene, (C14H29-) (isomer)
245.88 35 22.87 Unknown (91, 161, 105, 203,
274)
247.76 36 22.95 Unknown (91,
274)
251.76 37 23.12 'Unknown (91,
258.82 38 23.42 Unknown (91,
274)
270.59 39 23.92 Benzene, (1-methyl tridecyl-)
f,9,h
147, 105, 217,
133, 105, 274)
119, 105, 117,
490,189
409,880
520,287
57,019
611,030
232,582
167,829
162,458
211,169
41,489
271,660
46,691
38,543
40,472
51,755
47,787
58,517
287.9
240.7
305.6
33.5
358.9
136.6
98.6
95.4
124.0
24.4
159.6
27.4
22.6
23.8
30.4
28.1
34.4
(continued)
-------
TABLE E.21-1 (continued)
RRI
Retention
Peak time,
No. min
Compound
Peak area,
total counts
Amount Amount
detected, spiked. Recovery,
pg/L pg/L percent
Total concentration
4,872.5
W
l
VO
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 2% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 22.6 jjg/g relative to the
anthracene-d10 internal standard.
Anthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 166.5 |jg/g in original sample.
oeuterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-djo, internal
standard.
"Tentative identification, not confirmed with authentic standards.
llajor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
Summation of concentrations of detected compounds, excluding standards.
^Retention time less than biphenyl.
-------
TABLE E.21-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT B143S
Parameter
% Moisture3
TCO , mg/g (ppt)
GRAV , mg/g
TCG, mg/g
Organics detected by GC/MS , mg/g
Value
78.4
1.74
4.20
3.67
4.87
Carbon balance
% Extractabled 5.94 x 10"1
% Chromatographable f 5.41 x 10"1
% Extractables identified1 82.0
-Reported as 1 -Free Wt x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
cFrom Table E.21-1.
*% of carbon extractable = TC°1QaGRAV x 100
^ TfO + TCC
e% of carbon Chromatographable = 103 x 10°
% extraetables identified
_ Identified organics detected v inn
TCO + GRAV
E-93
-------
TABLE E.21-3 SEDIMENT ICAP METALS ANALYSIS
RESULTS, PLANT B143S
Concentration3,
Metal pg/g
Ag <22
Al 21,000
B 68
Ba 210
Be <0.40
Ca 10,200
Cd 8.5
Co 84
Cr 1,660
Cu 130
Fe 77,800
Mg 10,000
Mn 900
Mo <25 -
Na 371,000
Ni 120
P 450
Pb 60
Sb <54
Si 1,580
Sr 290
Ti 21,500
V 4,760
Zn 380
Original sample solids
content was 21.6%.
E-94
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TABLE E.22-1. ORGANIC COMPOUNDS IN SEDIMENT C169S, CORRECTED FOR BACKGROUND
a,b
W
I
«x>
VI
RRI
202 . 84
Peak
No.
F
. 9
Retention
time,
min
Compound
Peak area,
total counts
Amount Amount
detected, spiked, Recovery,
Mg/L Mg/L percent
Internal standard
20.62
Anthracene-d10
518
,075
66
.6
Recovery standard '
298.58
397.66
498.24
la
i
Ic
'13
14
18
6.08
7.20
14.27
24.67
28.48
32.48
Phenol-d5
1 , 2-Dichlorobenzene-d4
Biphenyl-d10
Pyrene-d10 .
Chrysene-d12
Perylene-d12
3
3
68
117
71
69
,557
,001
,843
,890
,615
,053
2
1
8
12
14
14
.4 35.6 6.8
.3 35.9 3.7
.9 33.5 26.5
.9 34.3 37.6
.3 . 33.7 42.4
.1 34.0 41.4
Organics detected '"'
106.18
123.09
133.01
158.05
169.92
181.79
183.25
206.38
209.46
237.82
2
3
4
5
6
7
8
10
11
12
14.73
15.77
16.38
17.92
18.65
19.38
19.47
20.77
20.90
22.10
n-C14-alkane
Unknown
Unknown
hydrocarbon
hydrocarbon
(71,
(57,
57,
71,
85)
85)
n-C16-alkane
Unknown
Unknown
Unknown
Unknown
Unknown
Unknown
85)
hydrocarbon
hydrocarbon
hydrocarbon
hydrocarbon
hydrocarbon
hydrocarbon
(57,
(57,
(71,
(57,
(71,
(57,
71,
71,
57,
71,
57,
71,
85)
85)
85)
85)
85)
28,
19
19
57
39
27
27
56
14
27
12
,319
,884
,225
,354
,514
,990
,403
,008
,470
,635
2
2
7
5
3
3
7
1
3
1
.5
.6
.4
.1
.5
.6
.3
.8
.5
.6
(continued)
-------
TABLE E.22-1 (continued)
W
vO
RR1
Peak
No.
Retention
time,
min
Compound
Organics detected (continued) '^
407.79
421.36
479.40
502.01
553.52
561.56
15
16
17
19
20
21
28
29
31
.88
.42
.73
32.63
34
35
.68
.00
Unknown
(57, 71
Phthalate plus
Unknown
Unknown
Unknown
Unknown
(57, 28
(57, 71
(28, 57
(28, 32
, 85)
unknown
. 71,
, 85,
. 71,
, 85,
85)
28)
85)
71)
Peak area,
total counts
,h
2
14
12
28
21
21
,711
,804
,126
,796
,071
,032
Total concentration
Amount Amount
detected, spiked, Recovery,
Mg/L pg/L percent
0
1
1
3
2
2
51
.3
.9
.6
.7
.7
.7
.8
Background correction based on reagent and/or method blank analysis. Appendix J.
Includes all substances that were found at or above a 3% threshold on the BATCH peak identifica-
tion program in the Hewlett-Packard system and are greater than 0.3 |jg/g relative to the
anthracene-d10 internal standard.
CAnthracene-d10 internal standard added immediately prior to analysis; 50 mg/L injected equivalent
to 66.6 ug/g in original sample.
i)euterated recovery standard (spike), added to original sediment after freeze drying and before
Soxhlet extraction.
Quantitated relative to area of major ion for authentic standard.
Estimated concentration based on area of total ion relative to that of anthracene-d10, internal
standard.
^Tentative identification, not confirmed with authentic standards.
najor masses of unknown compounds are listed in parentheses according to decreasing intensity.
Possible molecular ions are underlined.
"'"Summation of concentrations of detected compounds, excluding standards.
•'Retention time less than biphenyl.
-------
TABLE E.22-2 ORGANIC CARBON DISTRIBUTION
FOR SEDIMENT C169S
Parameter Value
% Moisture3 69.7
TCO , mg/g (ppt) 0.41
GRAV, mg/g 6.40
TCG, mg/g 3.49
Organics detected by GC/MS , mg/g 0.05
Carbon balance
% Extractabled 6.8 x 10'1
% Chromatographable f 3.9 x 10*"1
% Extractables identified 0.7
'Reported as 1 -["•*&"% Wt] x 100
Modified TCO in that run was started at C8
instead of C7 to eliminate hexane solvent.
GFrom Table E.22-1.
/£ of carbon extractable = TnS~. x ^0
e TCO + TCG
% of carbon Chromatographable = 1Qa x 100
% extractables identified
_ Identified organics detected
TCO + GRAV
E-97
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TABLE E.22-3 SEDIMENT ICAP METALS ANALYSIS
.RESULTS, PLANT C169S
Concentration3,
Metal pg/g
Ag
Al
B
Ba
Be
Ca
Cd
Co
Cr
Cu
Fe
Mg
Mn
Mo
Na
Ni
P
Pb
Sb
Si
Sr
Ti
V
Zn
<22
39,500
45
210
0.95
12,800
7.3
41
970
320
27,800
13,800
550
<25
38,800
116
1,440
210
<54
3,310
95
1,420
150
680
aOriginal sample solids
content was 30.3%.
E-98
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