EPA 560/6-77-031
   MULTIMEDIA LEVELS
             MERCURY
            SEPTEMBER 1977
        ENVIRONMENTAL PROTECTION AGENCY
          OFFICE OF TOXIC SUBSTANCES
            WASHINGTON, D.C. 20460

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EPA-560/6-77-031
                             MULTIMEDIA LEVELS
                                 MERCURY
                              September 1977
                                BATTELLE
                          Columbus Laboratories
                             505 King Avenue
                          Columbus, Ohio  43201
                           Vincent J.  DeCarlo
                             Project Officer
                         Contract No.  68-01-1983
                      ENVIRONMENTAL PROTECTION AGENCY
                        OFFICE OF TOXIC SUBSTANCES
                         WASHINGTON, D.C.   20460

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                       NOTICE
     This report has been reviewed by the Office of
Toxic Substances, Environmental Protection Agency, and
approved for publication.  Approval does not signify
that the contents necessarily reflect the views and
policies of the Environmental Protection Agency.  Mention
of tradenames or commercial products is for purposes of
of clarity only and does not constitute endorsement or
recommendation for use.
                                                                    I'
                        ii

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                               PREFACE
     This review of the environmental levels of mercury was conducted
for the U.S. Environmental Protection Agency, Office of Toxic Substances,
under Contract No. 68-01-1983.  It involved (1) the review and evaluation
of existing monitoring data and (2) development of an integrated data
package.  Information sources were identified from computerized and
manual searches.  Data were obrained from specialized data centers;
university programs; federal programs, state programs; the Smithsonian
Science Information Exchange; EPA's Storet, SAROAD, and NASN data centers;
the USGS and Fish and Wildlife Service; and from readily available open
literature.

     Information gathered included mercury concentrations in air, surface
and drinking water, groundwater, soil, food, sediment, sludge, aquatic and
terrestrial organisms, human tissues, and body fluids.  Details were
obtained on the methods of sample collection, interferences, meteorological
data,  and analytical methods employed.
                            iii and iv

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                           TABLE OF CONTENTS
1.  INTRODUCTION	1-1

          Physical and Chemical Properties	1-1
          Production and Uses of Mercury	1-1
          Sources of Environmental Contamination by Mercury  ....  1-5

2.  MERCURY LEVELS IN THE ENVIRONMENT	2-1

          Air	2-1
          Water and Sediments	2-10
          Drinking Water	2-23
          Sludge	2-25
          Rocks and Soils	2-27
          Terrestrial Biota  	 	  2-31
          Aquatic Biota 	  2-43

3.  BEHAVIOR IN THE ENVIRONMENT	3-1

          Transformation in Nature	3-1
          Geochemical Cycle of Mercury	3-6
          Food Chain Transport	3-14

4.  OCCURRENCE OF MERCURY IN FOOD	4-1

          Mercury Levels in Fish	4-1
          Mercury Levels in Foods Excluding Fish	4-2
          Mercury in the Market Basket and Total Diet	4-11
          Estimated Dosage	4-12

5.  MERCURY IN MAN	5-1

          Mercury Vapor 	  5-1
          Mercuric Mercury	5-3
          Alkylmercurials 	  5-6
          Mercury Level in Human Tissues	5-7

6.  REFERENCES	6-1

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                              FIGURES

Number
 1.1    Commercial U.S. mercury deposits

 1.2    Projected mercury consumption in the United States by end
          use
                                                                     1~6
 1.3    Projected mercury consumption in the United States by end
          use, Year 2000	1-7

 1.4    Past and probable future trends in final disposal of
          mercurials	1-12

 2.1    Theoretical saturation concentrations of mercury in air . .   2-2

 2.2    Location of hydrologic benchmark stations maintained by
          the U.S. geological Survey	2-13

 2.3    Mercury levels in U.S. surface waters,  1970-1974	2-18

 2.4    U.S. Geological Survey classification of major drainage
          basins in the United States	2-19

 2.5    Mercury levels in Baltimore Harbor sediments	2-21

 2.6    Frequency distribution of mercury in Great Lakes sediments.   2-24

 2.7    Levels of mercury in fishes, National Marine Fisheries
          Service Microconstituent Resource Survey	2-54

 3.1    Cycle of mercury interconversions in nature 	   3-2

 3.2    Pathways of mercurial breakdown and methylation in nature .   3-5

 3.3    Generalized geochemical cycle of mercury in natural
          systems	3-7

 3.4    Tentative present-day cycle of mercury	3-9

 5.1    Mean Blood Levels of Mercury Versus Time-Weighted Averages
          of Mercury in Air	5-4

 5.2    Urine Levels of Mercury Versus Time-Weighted Averages  of
          Mercury in Air	5-4

 5.3    Elimination of total mercury from hair,  blood  cells, and
          plasma in six subjects	5-8
                                 VI

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                                TABLES

Number                                                               Page

 1.1    United States Domestic Mercury Production 	  1-3

 1.2    Consumption of Mercury in the United States	1-4

 1.3    Total Mercury Losses in 1973 for Conterminous U.S. by
          Sector and SIC Category	1-8

 2.1    A Comparison of Mercury Concentrations in the Air as a
          Function of Species and Location	2-4

 2.2    Elemental and Total Atmospheric Mercury Concentrations in
          Three North Carolina Cities 	  2-5

 2.3    Summary of Atmospheric Mercury Measurements at Selected
          U.S. Locations	2-7

 2.4    Ambient Outdoor Mercury Concentrations in Selected U.S.
          Cities	2-10

 2.5    Summary of California Atmospheric Mercury Measurements. .  .  2-11

 2.6    Mercury Levels in Surface Waters of the United States . .  .  2-14

 2.7    Mercury Levels in Groundwater of the United States	2-16

 2.8    Summary of Mercury Concentrations in U.S. Waters	2-17

 2.9    Mercury Concentrations in the Ohio River and Some of its
          Tributaries	2-22

 2.10   Mercury in Public Drinking Water Supplies 	  2-26

 2.11   Mercury Content of Rock Types	2-28

 2.12   Concentration of Mercury in Urban and Suburban Soils. . .  .  2-30

 2.13   Mercury Levels in Terrestrial Mammal Hair	2-33

 2.14   Mercury Levels in Starlings (Sturnus vulgaris);  1970, 1971,
          and  1973	2-35

 2.15   Mercury Levels in Woodcock (Philohela minor)  liver,
          1970-1971	2-39

 2.16   Mercury Levels in Black Duck and Mallard Wings (Amar
          Rubripes and A.  platyhyncher). 1969-1970	2-40
                                 Vll

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                           TABLES (Continued)

Number                                                               Page.

 2.17   Mercury Levels in Duck Breast Muscle,  1970-1971 	   2~42

 2.18   Mercury Levels in Birds of Lake St.  Clair,  Michigan ....   2-44

 2.19   Distribution of Mercury in Tissues of  Oregon  Pheasants.  .  .   2-46

 2.20   Mercury and Methylmercury Content of Pheasant Breast  Tissu
          Tissue	2~46

 2.21   Levels of Mercury in  Fish from Region  1,  National  Pesti-
          cides Monitoring Program,  1969, 1970, and 1972	2-48

 2.22   Levels of Mercury in  Fish from Region  2,  National  Pesti-
          cides Monitoring Program,  1969, 1970, and 1972	2-49

 2.23   Levels of Mercury in  Fish from Region  3,  National  Pesti-
          cides Monitoring Program,  1969, 1970, and 1972	2-50

 2.24   Levels of Mercury in  Fish from Region  4,  National  Pesti-
          cides Monitoring Program,  1969, 1970, and 1972	2-51

 2.25   Levels of Mercury in  Fish from Region  5,  National  Pesti-
          cides Monitoring Program,  1969, 1970, and 1972	2-52

 2.26   Levels of Mercury in  Fish from Region  6,  National  Pesti-
          cides Monitoring Program,  1969, 1970, and 1972	2-53

 2.27   Levels of Mercury in  Plants  of Lake  Powell, Arizona ....   2-58

 3.1     Global Cycling  of Mercury 	   3-8

 3.2     Maximum Mercury Concentration in Air Measured at Scattered
          Mineralized and Nonmineralized Areas of the Western
          United States 	   3-11

 3.3     Concentrations  and Concentration Factors  of Mercury in
          Water  and  Edible Aquatic Organisms	3-15

 3.4     Biological Magnification  of Mercury  in the  Aquatic  Food
          Chain	3-15

 4.1     Summarization of  Fish Type by  Total  Mercury Content ....   4-3

 4.2     Mercury  Residues  in Canned Sea Food	4-4

 4.3     Mercury  Content of  Selected Food Fish	4_5
                                viii

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                            TABLES (Continued)

Number                                                               Page

 4.4    Mercury Content of Fresh Fruits and Vegetables
          Commercially Available in Denton, Texas 	  4-7

 4.5    Mercury Content of Foods (Excluding Fish) 	  4-8

 4.6    Mercury Content in Foods: National Pesticides Monitoring
          Program Market Basket Data	4-13

 4.7    Summary Values for Mercury Levels in Nonfish Commodities. .  4-14

 4.8    Estimated Mercury Dose Through Standard Diets 	  4-15

 4.9    Mercury Exposure from the Diet of Fish Eaters	4-17

 4.10   Mercury Exposure from the Diet of Weight Watchers	4-17

 5.1    Mercury Vapor Concentration in Naval Dental Operating
          Rooms	5-3

 5.2    Mercury Content of Selected Human Tissue	5-10

 5.3    Concentration of Mercury in Human Hair—Persons of Limited
          and Known Exposure	5-11
                              ix and x

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                           EXECUTIVE  SUMMARY
      A determination  and  evaluation  of  environmental  levels of mercury,
 based on  a review of  the  literature  and other  information  sources, is
 presented in  this report.

      The  annual  emission  of mercury  to  the environment  is  approximately
 1,525 metric  tons (1973 estimate).   About 4 percent of  this comes from
 mining and smelting of mercury  and other ores,  21 percent  comes  from
 manufacturing and processing  operations, and 65 percent comes from the
 use  and disposal of mercury products.   The remaining  10 percent  comes
 from inadvertent releases  resulting  from mercury being  a contaminant in
 other substances, especially  coal and other fuels.  The major uses of
 mercury are in electrical  apparatus  and electrolytic  preparation of
 chlorine  and  caustic  soda.

      The  major portion  (63 percent)  of  these emissions  is  land-destined
 waste,  followed  by air emissions  (31 percent)  and waterborne effluents
 (6 percent).   The release  of  mercury from natural sources  to air and
 water is  double  the man-related losses  to these media.

      The  ambient concentration  of mercury in urban air  is  in the range
 of 2  to 60 nanograms  per cubic  meter.   Higher  levels  are observed near
 mercury mines  and geothermal  steam fields, in  the plumes from power plants
 and  incinerators,  and in the  environs of sewage treatment  plants.  Indoor
 air  concentrations  can also be  elevated in laboratories and dental offices
 where mercury  is used.

      The  natural background level of mercury in surface and underground
 water is  below 0.5  ppb.  In areas of mercury mineralization and  in some
 industrialized areas, values  up to a few ppb are observed.  The  concen-
 trations  of mercury in sediments reflect industrial inputs and are on
 the order  of parts  per million.  The highest levels are found near the
 sediment  surface.

      In U.S. drinking water,  mercury levels are normally less than 2 ppb.

     Mercury concentrations in  sludge resulting from  the treatment of
wastewater are of the order of  a few ppm, and  the variability among U.S.
 cities  is not  great.  Thus, in  agricultural applications of sludge,
mercury has not  been  a great  concern.

      In U.S. soils not disturbed by  man mercury concentrations approxi-
mate  those found  in rocks, 0.02 to 0.4  ppm.  Higher levels are found in
urban areas than in the surrounding  suburbs.   The mercury  content of golf
                                  xi

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 course soils can be up to several hundred  ppm because  of  the  use  of
 mercurial fungicides.   Croplands  do  not  appear to have elevated mercury
 levels.

      The levels of mercury occurring in  birds and mammals are quite
 variable, depending on food habits and environmental conditions.  Levels
 may vary in or between regions  because of  different amounts of naturally
 occurring mercury in the  environment, its  mobility in  the environment,
 and the  amounts artificially introduced.   Herbivorous  animals tend to
 have lower levels of mercury than omnivorous  or carnivorous species.
 Granivorous species with  access to mercury-coated seeds have  elevated
 levels.   Predators associated with wetlands or aquatic habitats usually
 have the highest mercury  levels.   Mercury  appears to concentrate  in  the
 liver and hair or feathers.

      Levels of mercury in the tissues of aquatic biota appear to  be
 consistently highest in industrialized Atlantic coastal environs—both
 freshwater and marine.  Regionally high values also occur in  the  states
 of  the Pacific Northwest,  because of both  mining activity and the
 leaching of soils naturally high  in  mercury content.   Other generally
 isolated incidences of contamination occur in many states of  the  U.S.
 These levels are most  often associated with a point source of mercury
 emission,  such as sewage  treatment and chlor-alkali plants, or nonpoint
 source runoff from agricultural or other use  of mercurial fungicides.
 The highest accumulations  occur in fishes  at  the top of the trophic
 structure,  the sport fishes  and piscivores.

      The behavior of mercury in the  environment is complex due to its
 existence  in three  oxidation states  and  the fact that  it  reacts to form
 literally  hundreds  of  compounds,  both organic and inorganic.   The
 residence  times  for mercury  in  the four compartments of the environment
 are:  land,  1,000 years; atmosphere,  60 days;  ocean, 320,000 years; and
 sediments  2.5  x  10   years.   The terrestrial system retains approximately
 50  percent  of  the total mercury it receives,  and the remainder reaches
 the  aquatic  system through  runoff and leaching.  Most  of  the  mercury
 reaching the soil is confined to  the top 3 cm.  About  1 percent is taken
 up by  plants.  The  majority  of  all forms of mercury in water  accumulate
 finally  in  bottom sediment,  where they may be taken up by bottom
 feeders  and  enter the  food  chain.  Methylmercury is synthesized in the
 environment  by both biological  and chemical processes  and is  the
 predominant  form found  in  aquatic organisms.

     The Food  and Drug  Administration's Total Diet Studies have provided
 continuous monitoring  of mercury  levels in foods since 1971.  Of  the 12
 food groups  examined in this  program, only the meat, fish, and poultry
composite has  regularly contained  measurable  amounts of mercury.   The 11
remaining  food groups have generally contained less than 0.01 ppm
mercury.  "Market-basket"-type  surveys in which a great variety of foods
are examined indicate that mercury concentrations in foods other  than
meats, fish, and  poultry products  are very low or nondetectable.
                                 XII

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     Estimates  of  daily  dietary  intake  of mercury  obtained  from FDA's
 Total  Diet  Studies for the  years 1971-1974 were  constant, averaging  about
 3  yg/day  over the  4-year period.

     Mercury levels in tissues of both  normal  and  exposed humans  show
 considerable variation.   Data  from localized studies  suggest  "normal"
 mercury levels  of  0.005  ppm in whole  blood, 10 ppm in hair, below 1  to  3
 ppm in kidney,  and below 0.75  ppm in  liver.  Higher levels  are  reported
 in chlor-alkali industry workers, in  dentists, in  people from areas  with
 natural mercury deposits, and  in urban  populations as compared  with  the
 rural.

     Biological half-lives  vary  considerably depending upon the chemical
 form of the mercury and  also according  to whether  the half-life is based
 upon the  whole  body or single  organs  or tissues.   Inorganic forms have
 the shortest half-life,  probably because of their  relatively  poor
 absorption  and  because their attachment to the plasma proteins  of the
 blood  (rather than the erythrocytes)  permits rapid excretion  from the
 body by the urine.  The  biological half-life of  inorganic mercury in
 blood  has been  estimated at 20 days,  while for the whole body,  about 40
 days seems  appropriate.   In comparison, vapor  and  alkylmercurials are
 more efficiently absorbed and  cross the blood-brain barrier,  permitting
 fixation  and accumulation in the brain  tissue.   Tracer studies  of human
 volunteers  following  inhalation  of radioactiver  mercury vapor have
 revealed  a  whole-body half-life  of about 58 days.

     Biological half-lives  in kidney  and brain are substantially  longer
 than those  for  the whole body  for all forms of mercury.  Evidence from
 primate studies suggests that the half-life of mercury in the brain  is
 probably  on the order of years.

     The  whole-body half-life of  methylmercury in  man is about  70 to 80
 days.  Tracer studies, calculations from decay curves following the
 elimination of  methylmercury from the diet, and  similar calculations
 based  on  the distribution of mercury  in hair of  poisoned individuals
 confirm this range.

     Absorption of  methylmercury  from the gastrointestinal  tract  is
 almost 100  percent  complete.  In  addition, efficient  mechanisms  of
 transport across the  blood-brain  barrier, affinity of alkylmercurials
 for neurological tissue  and  a long half-life permit accumulations in
 the brain when  intake of  mercury  is chronic.   In view of the  mechanism
 of biotransformation, a  potential  for methylmercury contamination of
 the food  chain  exists whenever mercury  is discharged  into food-producing
waters.
                                 xiii

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                                1.   INTRODUCTION
       Although mercury  (Hg)  is  a  rare  element,  ranking  16th  from  the bottom
 of  the list  in abundance  of  elements in  the  earth's  crust  (Goldwater,  1971),
 its unique properties have led  to a number of specialized  but  important uses
 by  man.   These uses, plus the natural  release of mercury from  ore bodies,
 make mercury an ubiquitous element occurring throughout the  environment  (in
 rocks, soil, water, and air, as well as  in plant and animal  tissues).
 PHYSICAL AND  CHEMICAL  PROPERTIES

       Mercury is  one of  the heaviest metals with a specific gravity of 13.5
 compared with 11.35 for  lead.   It  is the only metal liquid at ordinary room
 temperatures, having a freezing point of -40 C  (-40 F) and a boiling point
 of  357 C  (674 F).  It  has  a high vapor pressure, 0.002 mm of Hg at 26 C
 (79 F), so  that atmospheric concentrations at ordinary room temperatures are
 of  concern.   The  most  common ore of mercury is  cinnabar, mercuric sulfide,
 which  is easily reduced  by heating to liberate  elemental mercury.  As a
 liquid, mercury is handled in  flasks with the standard industrial unit for
 production, consumption, and pricing statistics being a 76-pound  (34.5 kg)
 flask.

       Mercury is  found in  three valence states: Hg(0), Hg(I), and Hg(II),
 and forms hundreds of  compounds, both inorganic and organic; the metal
 reacts with a wide variety of  oxidizing agents  to form Hg(I) and Hg(II)
 compounds and, not infrequently, mixtures of the two.

       The most important mercury compounds from an environmental viewpoint
may be categorized into  five groups:

         (1)   Metallic mercury  - liquid and vapor
         (2)   Inorganic  salts - sulfides, chlorides, nitrates, and oxides
         (3)   Alkyl compounds - those containing methyl or ethyl radicals
         (4)   Alkoxyalkyl  compounds - usually complexes
         (5)   Aryl compounds -  particularly phenymercurials.
PRODUCTION AND USES OF MERCURY

      Most commercial U.S. mercury deposits are found in the far western
states, primarily in Nevada and California (Figure 1.1).  Estimated U.S.
reserves represent only about 8 percent of the estimated world mercury
reserves of 180,000 tons (Cammarota, 1975).  Principal producing nations
                                    1-1

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 I
ho
                                        • MAZATZAL MTNS.
                                   • DOME ROCK MTNS.
                 HORSE HAVEN*
           BLACK BUTTE*
              BONANZA*
               ALTOONA
SULPHUR BANK**ABBOTT
          I   *REED   .-i.vj
     MIRABEL* •KNOXVILLE^
GREAT WESTERN*          '
     OAT HILL*
             SONOMA (GRE'AT EASTERN MT. JACKSON)
    GUAOALUPE •        \
   NEW ALMADEN*  .NEW|DR|A
       OCEANIC*
                                        Figure 1.1.   Commercial  U.S.  mercury deposits.

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 are  Algeria,  Italy,  Spain, Mexico,  Turkey, Yugoslavia, and the USSR.  The
 U.S.  is  a net importer  of mercury,  and  domestic primary production is
 limited  to a  small number of mines  (Smith, 1976) .  The number of mines
 has  decreased drastically in recent years, reflecting the gradual depletion
 of economically  recoverable ores  in the U.S.   The  declining  importance of
 U.S.  primary  production is illustrated  in Table 1.1 which shows operating
 mines and mercury production for  the past several  years.
             TABLE  1.1.   UNITED  STATES DOMESTIC MERCURY PRODUCTION
                                 1971     1972      1973      1974      1975a

Number  of producing mines          56       89        24        10         3

Production, metric tons            616      253        77        75       233
Production, number of  flasks    17,883    7,349     2,227     2,189     6,750

Price per flask  in
  New York  (duty paid)        $292.41    $218.28   $286.23   $281.69   $160.00
Source:  U.S. Bureau of Mines, 1976.

«a
 U.S. Bureau of Mines estimate.
The large Increase in 1975 resulted from the opening of a major new mine at
McDermitt, Nevada, which has an annual capacity of 20,000 flasks.  Only two
other mines were in operation in 1975, and present depressed prices for
mercury make it unlikely that U.S. production will increase significantly
in the near future.  It is evident from these data that losses of mercury to
the environment associated with mining and extraction have declined in
importance in recent years.

      Mercury consumption, by use, is given in Table 1.2 for the 1965-1975
period.  The major uses have been in electrical apparatus and electrolytic
preparation of chlorine and caustic soda (chlor-alkali).  Together, these
two uses accounted for 61 percent of U.S. consumption in 1975.

      The trend in mercury consumption in the U.S. has been downward since
the mid-sixties, primarily as a result of the recognition of its environ-
mental hazards.  Agricultural uses have declined significantly and are
expected to continue to decline.  Mercury catalysts are used in the produc-
tion of several plastics and synthetics; alternatives include organotins.
Use in dental preparations, electrical apparatus, and paints is expected to
increase with increasing population and Gross National Product (GNP).  Use

                                    1-3

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                          TABLE 1.2.   CONSUMPTION  OF MERCURY  IN THE UNITED  STATES
                                              (in number  of  flasks)
End Use
Agriculture
Amalgamation
Catalysts
Dental preparations
Electrical apparatus
Electrolytic prepara-
tion of chlorine and
caustic soda
General laboratory
use
Industrial and
control instruments
Paint: antifouling
Paint : mildew-
proofing
Paper and pulp
manufacture
Pharmaceuticals
Other
Total, Flasks
Metric Tons
1965
3,116
268
924
3,196
18,887
8,753
2,332
10,330
255
8,211
619
418
16,251
73,560
2,538
1966
2,374
248
1,932
2,133
17,638
11,541
2,217
7,294
140
8,789
612
232
16,359
71,509
2,467
1967
3,732
219
2,489
2,386
16,223
14,306
1,940
7,459
152
7,026
446
283
12,856
69,517
2,398
1968
3,430
267
1,914
3,079
19,630
17,453
1,989
7,978
392
10,174
417
424
8,275
75,422
2,602
1969
2,689
195
2,958
2,880
18,490
20,720
1,936
6,655
244
9,486
558
712
9,134
77,372
2,669
1970
1,811
219
2,238
2,286
15,952
15,011
1,806
4,832
198
10,149
226
690
6,085
61,503
2,122
1971
1,477

1,012
2,361
16,885
12,154
1,798
4,871
414
8,191
2
682
2,410
52,257
1,803
1972
1,836

800
2,983
15,553
11,519
594
6,541
32
8,190
1
578
4,280
52,907
1,825
1973
1,830

673
2,679
18,000
13,070
658
7,155
32
7,571

606
2,009
54,283
1,872
1974
980

1,298
3,024
19,678
16,897
476
6,202

6,807

597
3,514
59,479
2,052
1975a
600

689
1,773
16,235
15,223
278
4,102

6,926

431
1,735
51,105
1,763
Sources:   Van Horn (1975); Cammarota (1975)
Preliminary.

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 in  chlor-alkali cells  may remain essentially  around  present  levels  on  a
 replacement  basis  for  consumption in present  cells.   Chlor-alkali production
 capacity expansion is  estimated  to be more  likely via diaphragm cells  than
 mercury cells.

       The downward trend  in total mercury consumption is  not expected  to
 continue.  Projected consumption for 1985 has been estimated by the U.S.
 Bureau of Mines, as reported by  Van Horn  (1975)  and  shown in Figure 1.2.
 High  and low ranges are by the Bureau of Mines;  superimposed are "most pro-
 bable" estimates by Van Horn.  The U.S. Bureau of Mines  (Cammarota, 1975)
 subsequently estimated projected consumption  for the year 2000  broken  down
 into  six principal use categories, as shown in Figure 1.3.   This projection
 modifies some of the 1985 estimates.   Use in  electrical apparatus,  primarily
 batteries and mercury  lamps, is  estimated to  increase to  probably 25,000
 flasks/year, and perhaps  as much as 30,000.   At  the  same  time,  estimates
 of  mercury usage in instruments  and in paints have been reduced.  Use  in
 instruments  is  estimated  to range between 4,000  and  9,000 flasks.   Use in
 paints is projected to range between zero and 11,000 flasks,  depending on
 the extent of control  or  prohibition of this  use.  Either demands for
 "other" uses remain about the same, but others now include Pharmaceuticals,
 catalysts, and  agricultural uses.   Total use  is  estimated to range  between
 39,000 and 82,000  flasks, with the most probable value around 58,000 flasks.
 This  is nearly  the same as Van Horn's most  probable  estimate of  about  61,000
 flasks for 1985 and very  close to the average annual consumption for the
 years 1970 through 1975  (55,000  flasks).
 SOURCES OF ENVIRONMENTAL  CONTAMINATION
 BY MERCURY

      Mercury may enter the environment by a variety of routes: through
 man's use of the metal in chlor-alkali production, in electrical equipment,
 and in mildewicides and fungicides, etc.; through the combustion of fossil
 fuels and the smelting of nonferrous metals; and from natural sources such
 as ore deposits and geothermal steam fields.  Release to the atmosphere
 around ore deposits occurs because of the volatility of mercury.

      Estimates of 1973 mercury losses, tabulated according to Standard
 Industrial Classification (SIC) grouping have been published by the U.S.
 Environmental Protection Agency (Van Horn, 1975) and are reproduced in
 Table 1.3.

      In 1973, the total man-related losses in the U.S. were an estimated
 1,525 metric tons, of which 31 percent was lost to the atmosphere, 6 percent
 to water, and 63 percent to land.  Comparable amounts, 1,200 metric tons,
were released to the environment from natural sources (85 percent to the
 atmosphere and 15 percent to water).  The release of mercury from natural
 sources to air and water was double the man-related losses to these media.

      Losses through the combustion of fuel may be higher than those shown
 in this estimate.  According to Spittler (1976), experience is pointing to

                                   1-5

-------
               Low
I    I High
I
                                               Most Probable
Agriculture
Catalysts for
plastics
Dental
preparations

Electrical

Chlor- Alkali

Instruments

Paints
Pharmaceuticals
Other (including
laboratory use

Itl
^^^



lysyssss^

WWWW

flffift.
1





W/M

WM<




,
y#m<






WM,

yffi.




,



^v^^Ov^vv*

rfww.














\











,


I





















1







       2.5     5.0     7.5    10.0    12.5
                       Thousands of Flasks
                   15.0     17.5
                  20.0
Figure 1.2.  Projected mercury consumption in the United States
             by end use, 1985 (Van Horn, 1975).
                        1-6

-------
                             Low
                         I    I High
I
Most Probable
Caustic soda
 and chlorine
Dental supplies
Electrical
Instruments
Paints
Other
ssssssss^^
       I
                               10              20
                                    Thousands of flasks
                                                 30
              Figure 1.3.  Projected  mercury consumption in the United States
                           by end  use, Year 2000 (Cammarota,  1975).
                                        1-7

-------
        TABLE 1.3.  TOTAL MERCURY LOSSES IN 1973 FOR CONTERMINOUS U.S. BY SECTOR AND SIC CATEGORY

                                            (in thousands of kilograms)
i
00
Sector
I





II









III











SIC No.

1092M
1092P




1021
1031
3331
3333
3241
3274
3332



021
2911C

2911M
2951C
3312M
4091C

49110
4924C

Description
Mercury Mining and Smelting
Mercury mining
Mercury processing
(including secondary)
Subtotals

Other Mining
Copper mining
Zinc and lead mining
Copper smelting
Zinc smelting
Cement processing
Lime processing
Lead smelting
Subtotals

Unregulated Sources
Livestock
Fuel oil-residential, commercial,
commercial, industrial
Refineries
Tars and asphalt
Coke ovens
Coal-residential, commercial,
industrial
Utilities-oil and natural gas
Natural gas-residential,
commercial, industrial
Total
Air

0.01
7.84

7.85
(1.7%)

0.02
0.00
40.77
4.59
0.50
0.08
4.75
50.71
(10.8%)

0.00
16.94

1.15
1.10
7.16
9.97

11.99
15.46

Losses to
Water

0.00
0.00

0.00
(0.0%)

0.01
0.00
2.26
0.25
0.25
0.04
0.26
3.07
(3.5%)

0.0
0.00

0.00
1.67
0.51
0.00

0.00
0.00


Land

0.01
0.41

0.42
(0.0%)

0.08
0.01
2.26
0.25
1.76
0.29
0.26
4.91
(0.5%)

17.70
0.02

1.15
14.99
2.56
1.11

0.01
0.01

Total
Mercury
Lost

0.02
8.25

8.27
(0.5%)

0.11
0.01
45.29
5.09
2.51
0.41
5.27
58.09
(3.8%)

17.70
16.96

2.30
17.76
10.23
11.08

12.00
15.47

Total
Recycled

0.00
0.00

0.00
(0.0%)

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
(0.0%)

0.00
0.00

0.00
0.00
0.00
0.00

0.00
0.00


-------
TABLE 1.3 (Continued)
Total Losses
Sector
III



V
















VIIIA







SIC No.

4911C



2.5B
2819M
285 1M
2879M
2833M
2812
2261
2851F
38291M
3079P
3.0
3629M
36410M
36420M




2.5A
2879N
2879A
38291C
36292C
2641C
Description
Unregulated Sources (Cont'd)
Utilities-coal
Subtotals

Manufacturing and Processing
Caustic
Catalyst manufacture
Paint manufacture
Pesticide manufacture
Pharmaceuticals manufacture
Chlor-alkali
Textiles
Paint formulation
Control instrument manufacture
Catalyst usage
Other
Tubes /switches manufacture
Lamp manufacture
Battery manufacture
Subtotals

Final Consumption: Commercial
and Industrial
Urethane and miscellaneous
Nonagricultural pesticide use
Agricultural pesticide use
Control instrument consumption
Tubes/switches consumption
Lamp consumption
Air

40.71
104.48
(22.2%)

0.00
0.00
0.01
0.00
0.00
14.84
0.00
0.29
0.00
0.05
10.28
0.00
0.40
0.13
26.00
(5.5%)


0.12
4.39
0.00
16.54
7.53
6.07
Water

0.00
2.18
(2.5%)

7.61
0.02
0.20
0.06
0.02
2.93
0.15
0.35
0.00
0.10
10.23
0.00
0.00
0.05
21.72
(24.8%)


0.00
17.56
2.83
0.00
0.00
0.00
to
Land

4.52
42.07
(4.4%)

1.90
0.00
0.05
0.00
0.00
226.83
7.63
0.00
1.97
18.85
8.70
1.57
1.34
2.49
271.33
(28.1%)


2.22
21.95
16.02
107.50
46.26
37.31
Total
Mercury
Lost

45.23
148.73
(9.8%)

9.51
0.02
0.26
0.06
0.02
224.60
7.78
0.64
1.97
19.00
29.21
1.57
1.74
2.67
319.05
(21.0%)


2.34
43.90
18.85
124.04
53.79
43.38
Total
Recycled

0.00
0.00
(0.00%)

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
1.97
0.00
20.66
0.00
0.00
0.00
20.66
(14.1%)


0.00
0.00
0.00
82.69
0.00
0.00

-------
                                          TABLE 1.3  (Continued)
i
c
Total Losses to
Sector SIC No.
VIIIA

7391


VIIIB
2834C
2851C
36420C
8021


Total
Final Disposal
Sewage
Urban runoff
Natural Sources
Degassing
Description
Final Consumption: Commercial
and Industrial (Cont'd)
Laboratory usage
Subtotals

Final Consumption: Consumer Goods
Pharmaceuticals consumption
Paint consumption
Battery consumption
Dental applications
Subtotals






1,
Runoff and groundwater
Air


2.28
36.93
(7.8%)

1.07
173.61
69.71
0.93
245.29
(52.0%)
4ZL.J_6,

4.01
0.00

018.70
0.00
Water


5.92
26.31
(30.0%)

17 .77
0.00
0.00
16.65
34.42
(39.2%)
&LJH

19.92
11.70

0.00
188.30
Land


1.59
232.85
(24.1%)

2.09
9.14
403.30
0.00
414.53
(42.9%)
SJiiuJLl

22.88
0.00

0.00
0.00
Total
Mercury
Lost


9.79
296.09
(19.4%)

20.90
182.75
473.01
17.58
694.24
(45.5%)
1.525.07

46.80
11.70

1,018.70
188.30
Total
Recycled


12.98
95.67
(65.2%)

0.00
0.00
24.89
5.55
30.44
(20.7%)
14JL 77






     Source:  Van Horn, 1975.  Further refinements (as yet unpublished) have been made in these data,
              but the changes are small, ±5 percent.

-------
combustion processes  (coal and municipal or  industrial wastes) as a chief
source of ambient mercury.   In a discussion  of  fossil fuels as a source of
mercury pollution, Joensuu  (1971) pointed out that, although the concentra-
tion of mercury  in fuels  is  small,  fuels are consumed at an enormous rate,
consequently,  they must be considered as a possible significant source of
mercury released into  the environment.

      Joensuu  presented analyses for 36 American coals.  Mercury concentra-
tions ranged from 0.07 to 0.08 ppm  in some West Virginia coals to 20 to 30
ppm in other West Virginia and Wyoming coals.  Average of a large number of
Illinois coals was reported  as 0.21 ppm (Ruch et al., 1971).  If the nominal
average for coals burned  in  th.e U.S. is assumed to be 0.5 ppm, the average
annual consumption of 545 million metric tons (600 million tons) may release
up to 270 metric tons  (300 tons) of mercury  to the environment.

      The available data  indicate that almost all of the contained mercury
will be emitted  to the air.  Billings et al. (1973) determined a mercury
material balance on a large  pulverized coal-fired utility boiler, finding
that over 95 percent of the  mercury in the coal (0.3 ppm average) was
emitted into the flue gases.  Similar results were obtained by Klein and
Andren (1975)  in their investigation of the  Allen Steam Plant of the
Tennessee Valley Authority and by Anderson and Smith (1977) on the basis
of a year's observations  of  an Illinois power plant.  Thus, mercury input
to the environment from the  combustion of coal is an important source of
ambient mercury.

      Trends in  relative  mercury losses to the environment have been project-
ed by Van Horn (1975), as illustrated in Figure 1.4.  Discharges to water
have already decreased significantly and this trend will continue.  Dis-
charges to air have increased somewhat.  This is due primarily to the
increased use of fossil fuels with higher mercury content, and this is
expected to continue to increase.  Industrial discharges to land have
increased sharply, as the land has become the recipient of much of the
material previously discharged to water.

      The resulting trends in the observed concentrations of mercury in the
various environmental media  are not so easily deduced.  This is due not only
to deficiencies  in available data but also to the fact that mercury emissions
from natural sources are  comparable to man-related emissions.  Natural emis-
sions to air and water are double the man-related discharges to these media
and could mask small changes resulting from  man's activities.
                                    1-11

-------
 ra
SI
 u
 •
 u
 
-------
                     2.   MERCURY LEVELS  IN  THE  ENVIRONMENT
 AIR

 National Atmospheric  Monitoring Programs

       Concentrations  of  mercury in  the  atmosphere  have  not been  measured on
 a national  basis.   Mercury concentrations  are  not  included in  National Air
 Surveillance  Networks (NASN)  reports, nor  were data  found in the U.S.
 Environmental Protection Agency Storage and  Retrieval of Aerometric  Data
 (SAROAD)  computer  file.   The  apparent reason for this omission is that
 although atmospheric  mercury  may be largely  associated  with particulate
 matter,  some  may be present as  vapor.   Additionally, the high  ventilation
 rates  associated with the collector of  a Hi-Vol particulate sample may
 rerelease trapped  mercury back  to the atmosphere.  Although atmospheric
 mercury  concentrations based  on Hi-Vol  sampling have been reported,  they
 are  of dubious value  (Spittler,  1976).  Such results would appear to be
 biased on the low  side.

       Approximate  ranges of mercury vapor  concentrations in ambient  air
 drawn  from  Goldwater's (1971) overview  paper were  summarized by  Cooper
 et al. (1974)  as follows:

                                           Mercury Concentration,
                Type        Location       	ng/m3	

               Outdoor    Background           1-5
                           Urban                2-60

               Indoor      Indoor               100  - 200
                           Laboratory           200  - 10,000
                           Dental  office        10,000 -  100,000

While not national  averages,  these  appear  to well  represent the  results
from a variety of localized investigations.

      The explanation  for  the concentrations found in places which use
metallic mercury (laboratories and  dental  offices) is the high vapor
pressure of mercury.   The  vapor pressure increases rapidly with  temperature,
approximately doubling for  every  10 C increase.  The theoretical  saturation
concentrations of mercury  in air  have been calculated by Stahl (1969) .  As
illustrated by Figure  2.1,  at normal ambient temperatures these  are  in the
range of 5 to 20 milligrams per cubic meter.   While saturation is  an unlikely
concentration, these values are illustrative of the possibilities  of reaching
                                   2-1

-------
     12    16    20   24   28    32
                   Temperature, C
36   40
44
Figure 2.1.  Theoretical saturation concentrations of
             mercury in air (Stahl, 1969).
                         2-2

-------
elevated concentrations in unventilated rooms which are contaminated with
mercury.
Local Monitoring of Atmospheric
Mercury Concentrations

      Although there have been a few localized monitoring efforts in the
1970's there is still a paucity of data.

      Sampling and analytical methods and procedures have contributed to
the lack of reliable data on mercury concentrations in air.  As noted in
the preceding section, some of the local investigations have been based
on the analysis of Hi-Vol particulate samples.

      Some investigators absorbed mercury from the air in iodine monochloride
(IC1) solutions, an EPA standard method.  The initial version of the method
was found to give both imprecise and inaccurate results (Mitchell and Midgett,
1976) .  The method has been modified and improved by these same investigators
to give satisfactory results; however,  the reliability of earlier results
obtained by this method may be suspect.

      The preferred method for detecting mercury concentrations in air at
the nanogram per cubic meter level now  appears to be based on the trapping
of mercury as an amalgam on silver wool or on a gold screen.  The use of
two duplicate air streams permits the collection of elemental mercury on
silver wool in one side; a pyrolyzer converts mercury compounds in the
other stream to elemental mercury, which is also collected on silver wool.
This method provides data on both elemental and total mercury (Spittler,
1976).  The analytical method now generally used is based on revolatiliza-
tion of the collected mercury sample and passage through a gas optical cell
in a flameless atomic absorption spectrometer.

      Soldano et al. (1974, 1975) described the results of a survey of
airborne mercury concentrations in the  environs of sewage treatment plants,
using an adaptation of mercury speciation technique described by Braman et
al.  (1974).  This technique involves the stacking of four different materials
to collect different forms of mercury:

                Acid Chromosorb W - Inorganic mercury compounds
                Basic Chromosorb W - Alkyl mercury halides
                Silvered sand - Elemental mercury
                Gold-coated sand - Dialkyl forms of mercury

Results were presented for a number of  cities  (Table 2.1).  Large differences
in mercury concentration were found among the different cities and among
samples obtained within the same city.  The results ranee  from a  low  of
0.13 ng/m3 in a Utica, New York, sample to 600,000 ng/m3 in a Memphis,
Tennessee, sample.  Factors such as weather conditions, distance  from the
sewage plant, and elevation of the collection point are cited as  factors
influencing the results within a city.  All forms of mercury were found;


                                    2-3

-------
TABLE 2.1.   A COMPARISON OF MERCURY CONCENTRATIONS  IN THE
              AIR AS A  FUNCTION OF  SPECIES AND  LOCATION
Concentration, ng/m
Sample Location
Richmond (I)
Richmond (I)
Richmond (II)
Richmond (II)
Richmond (II)
Richmond (II)
Richmond (II)
TVA (Bull Run)
Oak Ridge TN
Knoxville TN
Knoxville TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Memphis TN
Greenville SC
Greenville SC
Greenville SC
Greenville SC
Greenville SC
Utica NY
Utica NY
Utica NY
Utica NY
Louisville KY
Louisville KY
Louisville KY
Louisville KY
Louisville KY
Set
No.
2
4
1
2
3
4
5
1
1
1
2
1
2
23
24
25
26
27
28
29
30
32
33
36
37
38
39
1
2
2
3
4
2
4
5
6
13
14
15
16
19
Acidic
(Inorganic-
Mercury)'3
20
2.13
0.25
0.88
0.78
1.25
2.50
0.43
1.98
3.75
1.30
210
1,428
2,930
2,090
1,667
1,237
2,875
4,225
140
3,250
186
275
3,280
15,875
3,750
3,800
0.12
7.75C
1.23
11.25
0.30
—
0.15
0.36
0.21
410
1,470
2,093
1,105
6,250
Basic
(Alkyl Mercury
Halides)b
	
62.50
8,750
0.53
0.50
9.50
0.85
0.83
1.38
107.50
21.75
2,262
9,525
1,050
4,180
3,800
7,625
33,250
2,625
130
3,730
383
198
8,580
1,032
6,250
19,000
0.68
—
—
67.50
6.50
0.13
3.25
0.80
0.95
308
2.950
9,300
13,159
42,500
Silver
(Elemental
Mercury)^
2.50
—
1.60
1.80
0.80
6.80
1.80
0.53
1.58
1.75
0.35
380
42,800
1,360
23,300
3,330
16.7
5,500
10,000
240
932
488
1,333
17,100
15,950
600,000
1,357
0.08
—
21.25
0.58
225
—
0.20
0.60
0.33
718
1,410
1,395
2,625
550
Gold
(Dialkyl
Forms)'3
__
—
—
—
—
—
0.50
0.33
2.75
4
0.55
285
4,750
3,330
13,950
1,380
285
3,700
4,530
210
605
465
4,780
515
6,975
10,000
452
1.50
	
1.55
3.50
25
—
3.50
—
1.68
410
15,300
465
1,262
2,375
 Source:  Soldano  et al.  (1974, 1975).

  All  are in the environs of  sewage treatment plants except TVA which is a
    coal-fired steam plant.

  Positive identifications  of the mercury forms were not made but were
    inferred from  the collected fractions.
  Interference.
                                  2-4

-------
and the authors indicate that the mercury concentration-distance dependency
varies with the mercury form, a less volatile form (elemental mercury)
declining in concentration with increasing distance from the plant, and the
form collected in the basic fraction (highly volatile alkyl mercury halides)
increasing in concentration with increasing distance.  The results are
compared with the lower concentrations obtained near a TVA coal-fired steam
plant at Bull Run, and the conclusion is drawn that the results provide
evidence for mercury and organic mercury pollution of the air that can be
attributed to municipal sewage treatment facilities.  The sewage treatment
facilities may constitute a means of concentrating elemental mercury and
reemitting it, in combination with the organic products characteristic of
sewage systems, into the air (Soldano et al., 1975).  The authors correlate
their maximum observed organic mercury concentrations with the population
load on the sewage facility, Oak Ridge and Utica being the smallest and
Memphis the largest.

      The largest variety and number of recent analyses are those generated
by Spittler (1976).  In the first set of analyses of locations in North
Carolina sampled in 1972, the double-air-stream instrument was used,
permitting analysis for both elemental and total mercury.  Results for
three cities are shown in 'Table 2.2.  The total mercury concentrations in
the three cities ranged from 4 to 8 ng/m3.
     TABLE 2.2.  ELEMENTAL AND TOTAL ATMOSPHERIC MERCURY CONCENTRATIONS
                 IN THREE NORTH CAROLINA CITIES
                                            Concentration, ng/m"
Date
1972 Location
October 3 Gary NC
4
5
6-8
October 3 Durham NC
4
5
6-7
October 3 Raleigh NC
4
5
6-9
Total
6.0
6.9
8.1
7.7
3.8
4.3
6.9
5.3
5.7
6.4
5.9
4.2
Elemental
6.0
6.8
6.1
5.7
4.7
4.4
4.8
3.5
6.0
6.8
5.8
4.1
Total Minus
Elemental
0.0
0.1
2.0
2.0
-0.9
-0.1
2.1
1.8
-0.3
-0.4
0.1
0.1
     Source:   Spittler, 1976.
                                    2-5

-------
       In  Spittier's  opinion, most airborne mercury will be found to be  in
 the  elemental  form unless some very special problem is known to exist or a
 specific  industrial  source  is under investigation.  In conformance with
 this  conclusion  (which appears to be in harmony with the data in Table  2.2),
 only  elemental mercury was  measured in subsequent analyses conducted in
 1975  and  1976.

       During 1975 and 1976  mercury analyses were conducted on a variety of
 samples mostly from  Massachusetts, Connecticut, Rhode Island, Vermont,  and
 Maine, with a  scattering of other samples from Indiana, Ohio, Illinois, and
 Florida.  Results of these  analyses are presented in Table 2.3.  Over 90
 percent of the samples yielded mercury concentrations in the range of 2 to
 60 ng/m3, the ambient urban air concentration reported by Goldwater (1971)
 and Cooper et al. (1974).   The most significant exceptions were a level of
 5,820 ng/m3 in a power plant plume in Fall River, Massachusetts, a level of
 275 ng/m3 in the plume from a burning dump in Middleton, Massachusetts, and
 200 ng/m  in an incinerator plume in Marlboro, Massachusetts.  Several
 samples were obtained in airports, but they did not yield results any higher
 than  ambient levels.

      A number of urban atmospheres were sampled by Cooper et al. (1974)
 using a gold-screen  collector.  Results are summarized in Table 2.4.  The
 two highest concentrations, El Paso, Texas, and Kellogg, Idaho, were from
 samples taken near nonferrous smelting operations.  Other samples ranged
 between 5.0 and 14.4 ng/m3.  In this same study, automobile exhaust gases
 were  sampled and found to contain between 95 and 160 ng/m3; the fuel was
 not analyzed.  These data suggest that transportation sources may be
 significant sources  of mercury emission due to the mercury content of
 petroleum.  This conclusion is supported by near-highway measurements by
 Cooper et al., which showed mercury concentrations from ca. 5 ng/m3 during
 low-traffic hours to 10 to  12 ng/m3 during peak traffic hours.

      A series of duplicate samples taken during 1973 in Chicago (Wroblewski
 et al., 1974) at nine sites, using a silver-wool collector, showed average
mercury concentrations to range from 10 to 38 ng/m ; the average for all
 sites was 22 ng/m3-  Individual samples ranged from 5 to 60 ng/m , and in
most instances, duplicate samples agreed within 20 percent.

       In  contrast to the above values, monthly composites of Hi-Vol filter
 samples collected during September-November, 1971, averaged only from 3.8
 to 4.1 ng/m3.  These data were considered to be evidence that either only
 a portion of the mercury was associated with particulates or losses were
 occurring during the collection of Hi-Vol samples.

      Jepsen (1973)  reported a series of measurements of atmospheric mercury
 concentrations in California, a state with a large fraction of the U.S.
 deposits  or mercury  ores, as well as a large portion of U.S.  geothermal
 steam fields.  He used a portable Barringer airborne mercury spectrometer
 (BAMS) and sampled by bus,  car, helicopter, and boat, utilizing traverses
 to locate, quantify, and map anomalous mercury plumes.   Several previously
 unreported sit^s of  mercury contamination associated with natural sources,
 such as mercury mines and hot springs areas, and cultural sources such as

                                   2-6

-------
TABLE 2.3.  SUMMARY OF ATMOSPHERIC MERCURY MEASUREMENTS
            AT SELECTED U.S. LOCATIONS
Location
Connecticut
Groton
Hamben
Hartford
Suburbs
Middleton
New Haven
Georgia
Atlanta, airport
Illinois
Chicago
Wheaton
Indiana
Indianapolis


Indianapolis , airport
Maine
Allagash River Basin




















Concentration , a
Date Time ng/m3

Aug. 5, 1975 49
Aug. 7, 1975 107
April 11, 1976 20
13
Aug. 7, 1975 29
14

Nov. 10, 1975 3.5

Oct. 4, 1975 7.0
Oct. 3, 1975 1.9 (2.4)

Oct. 5, 1975 4.4 (4.9)
Oct. 6, 1975 17.7 (19.4)
5.4 (8.4)
Oct. 7, 1975 33.2

Sept. 8, 1975 33
4.6
8
36
43
Nov. 3, 1975 12
20
8
16
15
4.3
11
9
Dec. 16, 1975 3
30
39
8
13
11
8.6
12
                            2-7

-------
TABLE 2.3.  (Continued)
Location
Massachusetts
Boston
Airport, inside
JFK parking lot
Over harbor, in plane
Ditto
Fall River, in power plant
plume
Framingham
In incinerator plume
Lexington
Marlboro









Incinerator, across street
Incinerator, in plume
Incinerator, 320 m downwind
Middleton
In plume from burning dump
90 m downwind of dump
1.6 km downwind of dump
3.2 km downwind of dump
8.0 km downwind of dump
Milton







Needham

Norwood, airport
Walpole
Date

Feb. 27, 1976
Nov. 10, 1975
Dec. 10, 1975
Aug. 15, 1975
Ditto


Dec. 9, 1975
Aug. 8, 1975
May 10, 1976
Aug. 5, 1975





-



April 11, 1976
Ditto
II

Nov. 28, 1975
Ditto
it
n
11
Aug. 21, 1975
Aug. 22, 1975
Ditto
Aug. 26, 1975
Ditto
Aug. 27, 1975
Ditto
Aug. 28, 1975
Aug. 6, 1975
Sept. 3, 1975
Nov. 28, 1975
Aug. 6, 1975
Concentration,3
Time ng/m^




0700-1000
1000-1200
























1530-1730
0800-1000
1000-1200
0900-1200
1200-1500
0800-1100
1100-1500
0800-1200





12
13
20
9 (14)
5.4 (7.
5820

5.5
42
9.8 (10
16 (20)
17 (13)
3.6 (3.
20.7
7.1 (8.
22.5
19.2
4 (6)
3 (4)
11 (12)
88
200
26

275
45
25
12.5
10
25
16.5
14.6
13.5
5.2
8.0
7.5
53
9.6 (10
4 (4)
15
10 (7.5)





3)




.3)


8)

6)






















.6)



          2-8

-------
                            TABLE 2.3.  (Continued)
      Location
Date
Time
Concentration,a
    ng/m3
Ohio
Cleveland, airport
Dayton, airport
Parma
Rhode Island
Providence
Vermont
Burlington
Rutland




Oct



Aug

Aug
Oct


Oct

. 7, 1975



. 5, 1975

. 13, 1975
. 16, 1975
Ditto
ii
. 17, 1975

6.9
18.7
4.9

105

62 (55)
1400-1500 7.5
1600-1700 17
2000-2300 3.3
0930-1200 20
Source:  Spittler, 1976.

 values in parentheses are replicates.
                                  2-9

-------
              TABLE 2.4.   AMBIENT OUTDOOR MERCURY  CONCENTRATIONS
                          IN SELECTED  U.S.  CITIES
City
El Paso TX
Kellogg ID
Oakland CA
Chicago IL
Memphis TN
Berkely CA
San Francisco CA
Tucson AZ
Seattle WA
Louisville KY
Nashville TN
Time of Sample
Collection
0945
1530
1140
1320
1040
1040
1745
1710
1805
1335
1655
Concentration ,
ng Hg/m3 air
29.6
21.5
14.4
13.0
11.8
10.5
10.3
9.5
8.6
5.9
5.0
Sample
Location
Industrial
Industrial
Freeway
Hospital
Industrial
Commercial
Freeway
Freeway
Residential
Commercial
Freeway
      Source:  Cooper et al., 1974.
 chlor-alkali  and  other  chemical  plants,  sewage treatment  plants,  and
 sanitary  landfills  were identified.   By  far the largest mercury concen-
 tration was detected  in a  steam  vent  at  the Geysers,  as shown  by Table
 2.5, which highlights the  anomalies from the reconnaissance  program,
 which covered over  30,000  km2.   Other natural sources with higher-than-
 average concentrations  were  near the  New Almaden mercury  mine,  as well as
 in the Clear  Lake area, near the still-active Abbott  mine, and  the closed
 Sulfur Bank mine.   However,  most of the  measurements  were at normal ambient
 levels, in the range  from  0  to 10 or  15  ng/m3,  comparable to levels in other
 states.

      Measurements  of mercury scrubbed from the air by rainfall have been
 made by several investigators.   In one of the most recent studies,
 Schlesinger et al.  (1974)  measured mercury and lead deposited by rainfall
 in an isolated area on  the western slope of a New Hampshire  mountain at
 elevations ranging  from 486  to 1,372  m (1,590 to 4,500 ft).  During the
 period June 30 to November 7, 1971, the  collected precipitation averaged
 0.06 ppb mercury, and the  mean deposition was calculated  to  be  0.23 ng/m2
 day.
WATER AND SEDIMENTS

National Monitoring Program

      Data on mercury in water are reported for two forms: dissolved and
total.  Dissolved mercury is determined on filtered (0.45 micron membrane)
samples, and provides an indication of what might occur in a treated or

                                   2-10

-------
                    TABLE  2.5.   SUMMARY  OF  CALIFORNIA ATMOSPHERIC MERCURY MEASUREMENTS
Site
Natural Sources
Abbott Mine
Clear Lake


The Geysers
New Almaden

Cultural Sources
Berkeley



Oakland-
Emeryville


Pittsburgh




San Francisco



Richmond

Date3

Feb. 12
Feb. 12

April 2
Feb. 15
Jan. 4
March 24

March 22
March 24
March 26
March 30
March 30
April 5
April 6

Feb. 11
Feb. 12
Feb. 25
March 22
April 1
March 18
March 19
Jan. 19-26
April 7,8
March 29

Time

P.M.
P.M.

P.M.
P.M.
P.M.
P.M.

A.M.
A.M.
P.M.
P.M.
P.M.
P.M.
P.M.

P.M.
A.M.
A.M.
P.M.
A.M. -P.M.
P.M.
A.M.
A.M. -P.M.
A.M. -P.M.
A.M.
P.M.
Wind

E
E

W
W



W
W
W
W
NW
W
W

E
NW
N
WNW
N




NW
W
Mercury
Background
Level ,
ng/m3

0
0

0
200-800
0
5-15

10

10
0
0
0
0

50
0
0
5
0
0
0
0
0
5
5
Mercury
Peak Value,
ng/m3

470
150

200
28,100
1,500
449

800
449
154
1,050
196
668
110
700
1,000
0
10
4,141

278
152
100
35
1,400
2,000
Comments

Low population density
Resort area, low population
density

Rural resort area
Rural


Light industrial-residential


Downwind of landfill
Light industrial
Industrial complex
Downwind of STP flare
Industrial area
Industrial area
On boat
Residential
Industrial-residential

Commercial area, quicksilver
products
Ambient measurements,
financial district
Residential, near primary school

Source:  Jepsen, 1973.
aDate not given, probably 1971.

-------
 filtered  water  supply  drawn  from the  same  source.   Total mercury represents
 the  amount  in the water-sediment mixture,  and  is  indicative of what might be
 included  in the food chain of  the aquatic  community (Durum et al.,  1971).
 Most of the analyses contained in this  report  are  of total mercury.

       A nationwide  reconnaissance of  selected  minor elements in the surface
 waters of the 50 states  and  Puerto Rico was  conducted by the U.S.  Geological
 Survey in cooperation  with the U.S. Bureau of  Sport Fisheries and Wildlife
 in the autumn of 1970  (Durum et al.,  1971).  There were  three kinds of
 samples:

                 •   Benchmark stations

                 •   Surface water sources for cities greater
                    than  100,000 population (or largest city
                    in  state)

                 •   Water courses downstream of major municipal
                    and/or industrial  complexes.

 The  presence of mercury  was  reported  in 19 percent  of the 722 published
 analyses  (Durum, 1974).   Dissolved mercury was detected  (greater than  1  ppb)
 in about  7  percent  of  the samples.  In  21  samples  mercury was found in both
 the  unfiltered  sample  (0.5 ppb  detection limit) and the  filtered (0.1  ppb
 detection limit) companion sample.  In  about half  of the  waters  in  which
 mercury was detected,  a  significant fraction of the mercury was  being
 transported in  the  particulate  fraction (Durum, 1974) .   The "benchmark"
 stations  were established by the Geological Survey  in the mid-1950's for
 purposes  of measuring  long-term natural trends in  stream  flow and water
 quality.  Benchmark stations are located toward the headwaters of tributary
 streams in  these basins,  in  generally undeveloped  areas,  and thus are
 presumably  fairly free from  contamination.  Their  locations are  shown  in
 Figure 2.2.  Examination  of  the  mercury analyses from the 49 benchmark
 stations  included in the  summary of the reconnaissance (Durum et al.,  1971)
 showed that  mercury was undetected  in 43 (88 percent).  California  and
 Nevada are  two  of the principal  areas of mercury mineralization, and it
 was  not surprising  that mercury  was detected at two  benchmark stations in
 each state  (0.8  to  6.0 ppb).  Except for these, mercury was detected only
 at single benchmark stations in  Maine (0.6 ppb) and  in Hawaii  (1.2  ppb).

      The Geological Survey, in  an unpublished report submitted  to  the U.S.
House of Representatives, Committee on Merchant Marine and Fisheries
 (Pickering,  1976),  summarized the  data  in  its computer storage file
 (accessed up to mid-January, 1976) on six  toxic substances,  including
mercury.   These data are  also presumed  to  include  the results  of the 1970
reconnaissance.   The data for mercury in surface waters  (streams, lakes,
and reservoirs)  and in groundwaters (wells and springs) have been extracted
from this  summary and are presented in  Tables 2.6 and 2.7,  and summarized
in Table 2.8.
                                   2-12

-------
N)
I
                                                 •4S     46.:	:rr;
                                                I        X	>        I
                              Figure 2.2.  Location of hydrologic benchmark  stations
                                           maintained by  the U.S. Geological Survey.

-------
TABLE 2.6.  MERCURY LEVELS IN SURFACE WATERS OF THE UNITED STATES
o
Dissolved Mercury
State
Alabama
Alaska
Arizona
Arkansas
California
Colorado
Connecticut
Delaware
Florida
Georgia
Hawaii
i Idaho
j^ Illinois
Indiana
Iowa
Kansas
Kentucky
Louisiana
Maine
Maryland
Massachusetts
Michigan
Minnesota
Mississippi
Missouri
Montana
Nebraska
Nevada
New Hampshire
New Jersey
New Mexico
NC
94
21
23
32
99
362
34
0
50
77
14
66
82
27
9
20
96
190
11
2
18
103
30
20
14
84
63
8
6
32
23
N>0d
88
9
17
28
75
196
34

40
42
12
60
79
20
9
14
78
122
11
2
18
91
22
16
8
72
56
8
6
32
14
N>2,
Ppb
24
1
0
6
16
3
1

1
3
0
11
1
0
4
3
4
2
0
1
2
16
1
2
1
7
17
0
1
1
0
Max,e
Ppb
56
4.0
0.6
4.3
12
4.5
3.8

3.6
2.0
1.2
8.7
2.2
0.8
24
3.0
7.5
2.3
0.6
5.0
4.7
196
2.6
2.7
4.3
9.0
44
1.8
3.7
2.8
0.5
Total Mercury
NC
47
42
13
100
157
122
30
0
296
40
10
43
26
23
15
29
95
155
16
2
21
79
41
67
28
82
66
15
22
53
77
N>0d
39
34
12
81
99
44
30

230
6
8
43
24
17
15
29
87
133
16
2
21
73
30
56
21
66
60
13
22
42
51
N>0.2,
Ppb
35
25
0
72
80
16
30

193
4
3
40
23
11
12
26
78
121
16
2
21
61
21
30
16
56
53
12
22
41
32

Max,e
Ppb
0.7
2.2
32
15
12
1.8
0.7

22
1.8
0.9
8.3
3.5
0.8
1.8
10
7.9
5.0
5.5
5.0
0.7
240
10
17
4.8
11
92
25
0.7
5.9
4.0
Mercury in
Bottom Material
NC
3
0
1
13
26
48
38
0
83
11
3
1
6
6
0
1
39
188
5
1
0
1
21
53
2
5
0
0
0
10
1
N>0d
4

0
13
22
47
36

69
8
3
0
5
0

0
32
170
3
0

1
21
31
0
5



8
0
Max,e
ppm
1.7

0.0
0.5
0.3
0.1
3.7

4.0
0.6
0.1
0.0
0.1
0.0

0.0
15
9.0
0.1
0.0

1.7
0.2
37
0.0
0.2



270
0.0

-------
                                           TABLE  2.6.  (Continued)
Ln
a
Dissolved Mercury
State
New York
North Carolina
North Dakota
Ohio
Oklahoma
Oregon
Pennsylvania
Rhode Island
South Carolina
South Dakota
Tennessee
Texas
Utah
Vermont
Virginia
Washington
West Virginia
Wisconsin
Wyoming
United States
„<
74
34
72
31
20
19
61
9
11
23
19
157
78
4
4
11
52
36
60
2,515
N>0d
74
28
58
31
12
14
61
9
10
14
18
142
50
4
4
10
52
25
23
1,918
N>2,
ppb
2
0
11
1
3
0
0
0
0
0
0
37
2
1
0
1
3
1
2
193
Max,8
ppb
17
0.8
76
6.0
12
1.0
1.3
0.5
0.3
1.0
0.6
160
7.0
3.4
1.6
2.1
5.0
2.0
2.4
196
N6
360
76
70
353
27
13
184
9
11
39
58
33
37
4
4
160
50
30
66
3,396
Total Mercury
N>0d
360
51
46
353
24
11
181
9
11
26
53
30
20
4
4
142
50
20
31
2,830
N>0.2,
ppb
360
48
34
351
18
7
172
9
11
19
35
24
12
4
4
121
50
10
22
2,472
Max,8
ppb
2,800
8.8
3.3
61
15
2.5
50
0.5
0.5
1.7
110
1.6
1.3
0.5
0.6
2,700
5.0
1.2
2.1
2,800
Mercury in
Bottom Material
NC
6
18
3
1
6
4
35
0
5
0
1
3
0
0
0
1
15
13
3
650
N>0d
6
17
0
0
6
0
33
0
4
0
0
1
0
0
0
0
7
7
0
559
Max,8
ppm
0.3
0.6
0.0
0.0
0.1
0.0
70

0.5

0.0
0.2



0.0
0.1
3.0
0.0
270
     Source:  Pickering, 1976.
      Filtered sample.
      Unfiltered sample.
     ^ = Number of stations for which data are available.
      N>0 = Number of stations with detectable mercury.
      Max = Maximum value found.

-------
       TABLE 2.7.   MERCURY  LEVELS  IN  GROUNDWATER OF  THE  UNITED STATES
Dissolved
State
Alabama
Alaska
Arizona
Arkansas
California
Colorado
Connecticut
Florida
Georgia
Idaho
Indiana
Iowa
Kentucky
Louisiana
Michigan
Minnesota
Mississippi
Missouri
Montana
Nebraska
New Hampshire
New Jersey
New Mexico
New York
North Carolina
North Dakota
Ohio
Oklahoma
Oregon
Pennsylvania
South Dakota
Tennessee
Texas
Utah
Virginia
Washington
West Virginia
Wisconsin
Wyoming
United States
NC
54
21
22
20
62
305
10
92
191
138
51
6
7
1
7
0
3
0
40
151
0
7
10
17
1
78
0
9
1
63
0
0
74
57
2
0
19
32
52
1,603
N>0d
48
7
11
7
21
77
8
68
92
77
34
6
6
1
6

0

20
67

6
7
17
0
45

1
0
63


53
29
2

19
22
17
837
•a
Mercury
N>2,
ppb
5
0
0
0
0
5
1
0
2
3
6
0
0
0
1

0

1
6

0
0
0
0
2

0
0
1


0
0
0

0
0
1
34
Max,6
ppb
10
0.6
0.2
0.4
1.0
5.3
5.0
1.7
5.6
4.3
9.8
1.0
1.4
1.4
13

0.0

2.0
13

0.5
0.7
1.0
0.0
2.4

0.1
0.0
11


1.9
1.0
0.2

0.5
0.9
2.1
13
N°
0
18
0
0
34
9
6
302
0
0
0
0
5
22
23
11
4
16
0
0
16
31
88
209
6
11
5
0
12
7
56
18
0
0
0
39
19
0
54
1,021
Total Mercury
N>0d

12


26
2
6
219




2
16
13
2
0
9


16
31
21
209
6
9
5

1
5
17
13



31
19

25
715
N>0.2,
PPb

6


18
1
6
148




1
11
8
1
0
8


15
31
8
209
5
3
5

1
5
13
5



4
19

13
544
Max,6
ppb

1.7


20
0.2
0.5
4.0




0.2
0.5
1.1
0.6
0.0
2.2


5.0
7.1
2.2
31
14
0.6
0.5

0.4
0.9
0.5
0.2



0.7
5.9

5.3
31
Source: Pickering, 1976.
o
 Filtered sample.

 Unfiltered sample.
CN = Number of stations for which data are available,

 N>0 = Number of stations with detectable mercury.

6Max = Maximum value found.
                                       2-16

-------
        TABLE 2.8.  SUMMARY OF MERCURY CONCENTRATIONS IN U.S. WATERS
                                 Surface Waters         Groundwater
                               Dissolved    Total    Dissolved    Total
    Number of states sampled        49        49          32        25

    Number of sites sampled      2,515     3,396       1,603     1,021
    Positive values
      Number                     1,918     2,830         837       715
      Percent                       76.3      69.3        52.2      70.0
    Values >2 ppb
      Number                       193                    34
      Percent                        7.7                   2.1
    Values >0.2 ppb
Number
Percent
Maximum value, ppb
2,472
72.8
196 2,800
544
53.3
13 31
      Dissolved values shown are based on the analyses of water passed
through a 0.45 micrometer filter; "total" values are for unfiltered samples
containing suspended sediment.  Dissolved values are compared with the 2
ppb maximum mercury level contained in the National Interim Primary Drink-
ing Water Regulations  (U.S. Environmental Protection Agency, 1975a).  For
"total" values the comparison is with the 0.2 ppb criterion recommended in
Water Quality Criteria 1972 for freshwater aquatic life and wildlife
(Rooney, 1973).  In only 2 to 8 percent of the samples did dissolved
mercury exceed the 2 ppb limit recommended for drinking water, but in 53
to 73 percent the total mercury exceeded the more stringent 0.2 ppb water
quality criterion recommended for mercury by the National Academy of
Sciences.  The Geological Survey data indicate that on the average, ground-
water quality with respect to mercury exceeds that of surface water.

      Data from a separate nationwide study of mercury in waters has been
summarized by Jenne (1972).  A majority of the samples analyzed contained
less than 0.5 ppb.  About 17 percent had mercury contents of 1 ppb or
greater.  Only 4 percent of the surface water samples contained in excess
of 10 ppb mercury.  The higher mercury concentrations were generally found
in small streams.  About half of the 43 samples from the Mississippi River
contained less than 0.1 ppb.  The mercury content of lakes and reservoirs
was scattered between less than 0.1 and 1.8 ppb.  With few exceptions, the
mercury content of qroundwater samples was below the limit of detection.

      In summarizing the present status of U.S. waters, Durum (1974) stated
that on the basis of the surveys to date in which the preponderance of

                                    2-17

-------
analyses show mercury to be below  the  limit  of detection,  the natural
background level  of  total mercury  in surface and underground water is
certainly below 0.5  ppb, and  is  probably  considerably lower than that.

      Confirmation of this conclusion  is  provided by the EPA STORE! data
for 1970-1974 presented in the 6th Annual Report on Environmental Quality
(Council on Environmental Quality, 1976)  shown in Figure 2.3.  The range
of mercury concentrations in  surface waters  is from 0.04 to 0.10 ppb.
                0.11—
C.06
O
•H .05
4-1
tfl .04
4J.03
§ .02
3.01
o
U  1970
                         1971
1972       1973
    Year
                                                   1974
                                                            1975
         Figure 2.3.  Mercury levels  in U.S.  surface waters,  1970-1974
                      (Council on Environmental  Quality,  1976).
Local Monitoring of Mercury Concentrations

      Local studies of mercury concentrations  in water  and  sediments have
been conducted in recent years in most of the  major  U.S.  drainage basins
(see Figure 2.4).  Many of these studies were  conducted to  investigate an
identified or suspected mercury-enrichment problem,  so  that averaging the
findings of the various studies can be misleading with  respect  to defining
average ambient concentrations.  Selected localized  investigations are
briefly summarized in the following paragraphs.

      The North Atlantic Basin(l)* is in one of the  most  highly urbanized
and industrialized regions of the country, and high  mercury concentrations
in water, and particularly in sediments, are possible.   Illustrative are
the sediment mercury concentrations determined in Baltimore Harbor by Villa
^Numbers in parentheses refer to drainage basins identified  in  Figure  2.4.

                                    2-18

-------
NJ
1
                   Figure 2.4.  U.S. Geological Survey classification of major drainage basins
                                in the United States.

-------
 and Johnson (1974),  as  shown in Figure 2.5.   The distribution of mercury was
 found to correlate with large industrial complexes,  and concentrations of up
 to 6 ppm were  observed.   The heaviest  concentrations can be seen to be
 located in the more  sheltered areas  away from the main channel and in the
 areas of manufacturing  industry or marine and commercial establishments.

       Roberts  et  al.  (1975)  has compiled state data  for mercury.  Sediment
 samples from Boston  Harbor were found  to have mean concentrations of 2 to 3
 ppm of mercury; sediment levels were generally higher at the sediment surface,
 and the highest mercury levels correlated with high  organic content.  In other
 Massachusetts  sediment  samples at Taunton River and  Muddy Cove,  means were 2
 to 36 ppm, with a range of 0.1 to 130  ppm.   Unlike the usual study in which
 there is no breakdown of the form of the mercury,  this study also determined
 methylmercury  content and found that only a  very small fraction, 0.1 to 0.5
 percent, was in the  methylmercury form.

       Similarly,  low fractions of methylmercury,  only 0.03 to 0.07 percent
 of total mercury,  were  found in the  Everglades (Andren and Harris, 1973).

       The Ohio River Basin(3)  is one of  the  major  U.S.  drainage  basins,
 especially whth respect to the population served,  and to the industrial
 concentration  within it.  The Ohio River Valley Water Sanitation Commission
 (ORSANCO)  has  monitored the  Ohio River and its major tributaries for some
 years, now including some of the heavy metals.   Recent (1975-1976) mercury
 results (Table 2.9)  are illustrative of  present levels  in the Ohio River as
 it passes through an industrialized  area.  Most of the samples are below the
 limit of detection and  the median value  is certainly below 0.5 ppb;  only
 one value exceeds  1  ppb.

       The St.  Lawrence  River Basin(4)  includes  the Great Lakes,  in which
 mercury concentrations  have  been studied extensively since about 1969,  when
 mercury contamination was discovered in  fish  taken in Canadian waters  of
 Lake St.  Clair.  Most of  the mercury contamination was  ascribed  to dis-
 charges from chlor-alkali plants; controls have  since been instituted  which
 have essentially eliminated  such discharges.

       Chau and Saitoh (1973),  in summarizing  the results of  a 2-year survey
 of  mercury concentrations in international Great Lakes  waters, concluded
 that the  distribution of mercury is  quite  uniform; the  mercury levels  in
 the majority (75 percent) of the samples  fell  within the 0 to 0.4  ppb  range
 for all lakes.   The mean  concentrations  for  915 water samples (surface,
 intermediate,  and bottom) from Lakes Ontario,  Erie,  Huron,  and Superior,
were  0.13,  0.17, 0.17, and 0.18  ppb, respectively.   Chau and Saitoh  classi-
 fied  the average mercury concentrations  as low compared with those for the
lakes and  reservoirs  of the  United States  considered by Jenne (1972).

      Chay  and Saitoh also concluded on  the basis  of a  comparison  of  their
work with  others that there  is  no direct  relationship between mercury
distributions  in the water and  sediments  of  the  Great Lakes.   The  sediment
mercury concentrations,  as a nonmobile phase,  reflect closely the  areas of
industrial  inputs, whereas water does not.  Confirmation of  the  correlation
of  sediment concentrations and  industrial  inputs can  be  found  in the  study

                                   2-20

-------
Figure 2.5.  Mercury levels in Baltimore Harbor sediments.
                               2-21

-------
           TABLE  2.9.   MERCURY CONCENTRATIONS IN THE OHIO RIVER AND  SOME OF ITS TRIBUTARIES
S3
Location
Illinois
Ohio River at Joppa
Indiana
Ohio River at Evansville
Ohio River at Uniontown
Kentucky
Licking River, Kenton County
Ohio River at Louisville
Ohio River at West Point
Green River at Sebree
Ohio
Ohio River at East Liverpool
Ohio River at Shady side
Muskingum River at Marietta
Ohio River at Belleville
Ohio River at Kyger Creek
Ohio River at Kenoga
Scioto River at Lucasville
Ohio River at Meldahl
Ohio River at Cincinnati
Little Miami River
Greater Miami River
Pennsylvania
Allegheny River at Oakmont
Monongahela River at St. Pittsburgh
Ohio River at South Heights
Beaver River at Beaver Falls
West Virginia
Ohio River at Pike Island
Ohio River at Willow Island
Date of Data Number of
Collection Samples

Oct. 1975-April 1976

Nov. 1975-April 1976
IT ri

n ti
Oct. 1975-April 1976
Nov. 1975-April 1976
Oct. 1975-April 1976

Sept. 1975-April 1976
II II
II It
II II
OcC. 1975-April 1976
Nov. 1975-April 1976
Oct. 1975-April 1976
II It
M II
Sept. 1975-April 1976
Oct. 1975-April 1976

Sept. 1975-April 1976
n n
ti n
n n

i n
n n

6

6
6

7
7
6
6

8
8
8
7
7
6
7
7
7
8
6

7
7
8
8

8
8
Frequency of
Detection ,
%

50

50
67

0
57
33
33

13
0
0
0
0
0
0
0
0
13
0

15
14
13
13

13
0
Range of Mercury
Concentration,
ppb

0.0-0.3

0.0-0.1
0.0-0.1

<0.5
0.0-0.2
0.0-0.1
<0.5

<0.5-0.5
<0.5
<0.5
<0.5
<0.5
<0.5
<0.5
<0.5
<0.5
<0.5-1.2
<0.5

<0.5-0.5
<0.5-0.5
<0.5-0.5
<0.5-0.5

<0.5-0.5
<0.5
    Source:  Ohio River Valley Water Sanitation Commission, 1975,  1976.

-------
 of  mercury contents  of  sediment  cores  from western Lake Erie by Kovacik and
 Waters  (1973) .   They determined  that  the mercury concentrations in sediments
 related directly to  the flow pattern  of Detroit  River water into the lake,
 carrying mercury from chlor-alkali plants upstream.   They found also that
 modern  sediments exhibited a surface  enrichment  zone of 1 to 4  ppm which
 decreased exponentially with depth to  the background level of 0.04 to 0.08
 ppm observed below about 15 cm in most cores.  Their data indicated that no
 change  has occurred  in background levels until modern times.

      Results of an  unpublished  EPA study (U.S.  Environmental Protection
 Agency, 1976a)  provide additional data on the  mercury contents  observed in
 403 sediment samples collected from harbors along the shores of the Great
 Lakes between Erie,  Pennsylvania, and  Duluth,  Minnesota.   As shown in the
 frequency distribution curve in  Figure 2.6, over 98 percent of  the samples
 were below 0.7 ppm,  and approximately  85 percent contained 0.2  ppm or less.
 Approximately 60 percent were below the detection limit of 0.1  ppm.   Only
 three samples,  all from the Great Lakes Naval  Training Base,  Illinois,
 exceeded 0.7 ppm (2.0,  3.0, and  14.0  ppm).

       Localized investigations in other basins provided similar patterns
 of mercury distribution.  The highest  levels of  mercury in surface waters
 tended  to be associated with periods  of low flow,  implying the  effect of a
 dilution factor during periods of high flow.  Elevated concentrations in
 sediments appeared to be related to inputs  from  nearby point sources.
 Sediment mercury concentrations  showed positive  correlations with organic
 content, small  particle size,  and sulfur content.   Undisturbed  sediment
 cores generally exhibit a decreasing mercury content with depth.   Although
 methylmercury analyses  are few in number,  the  evidence is consistent that
 the fraction of total mercury present  in sediments in this form is very low.
DRINKING WATER

       The U.S.  Public Health  Service  conducted  one  of  the  first broad-based
investigations  of  drinking water  quality  in  1969.   The  study was designed
to  give an  assessment of  drinking water quality in  urban and suburban areas,
and it may  be  considered  to comprise  the  baseline against  which current
concentrations  of  toxic substances  can be compared.  However, mercury was
not measured in this survey but was covered  in  a separate  study conducted
in  1970-1971 by the Environmental Protection Agency.   Results of this survey,
as  summarized by Hammerstrom  et al. (1972),  disclose that  261 (96  percent)
of  the 273  communities sampled had  less than 1  ppb  of  mercury; 11  (4 percent)
were between 1  and 5 ppb; and one exceeded the  5 ppb level.

       The above  indication of a mercury level above 5  ppb  led to an exten-
sive follow-up  sampling program in which  192 raw and finished water samples
were collected  at four plants in  this system over a 3-week period.  The
mercury concentration was found to be less than 0.25 ppb in 153 samples, and
less than 0.8 ppm in the remaining  30, casting  some doubt  upon the original
analyses.
                                     2-23

-------
100
 90
           0.1
0.2     0.3      0.4      0.5
  Mercury in Sediment, ppm
0.6
   Figure 2.6.  Frequency distribution of mercury in Great Lakes
                sediments (U.S.  Environmental Protection Agency,
                1976a).
                                  2-24

-------
       More recent data confirm the generally low level of mercury in drink-
 ing water supplies,  as indicated by Table 2.10 which summarizes  mercury
 monitoring data for  1975 and 1976 taken from the computer data base  main-
 tained by the Basic  Data Unit of the Water Supply Division of  the Environ-
 mental Protection Agency (Kent, 1976).   Of the total of 512 readings,  506
 were below 2 ppb; of these,  460 (89.8 percent) were below the  detection
 limit (0.5 ppb).   Only six samples (1.2 percent) exceeded 2 ppb.
 SLUDGE

       In sludge resulting from the treatment of  wastewater, mercury  has  not
 commonly been included in the metals for which analyses  have been  done,  and
 analytical data are fairly sparse.  However, the data  reported  below are
 widely dispersed geographically,  and are believed to be  representative of
 U.S.  sludge mercury levels.

       Salotto et al.  (1974)  reported the results of the  analysis by  the
 Environmental Protection Agency of about 100 digested  sludge samples
 collected from 33 wastewater treatment  plants in 13 states during  the
 period from the summer of 1971 through  1973.  They found the distribution
 of metals concentrations, including mercury, to  characteristically have  a
 lognormal distribution.   The arithmetic mean mercury concentration was 10
 ppm,  the geometric mean was  6.5 ppm, and the median was  6.6 ppm.   They
 also  found that the variation of  any metal in sludges  from a given plant
 was less than the plant-to-plant  variation.

       In another geographically broad-based  study, Furr  et al.  (1976)
 reported the analyses  for 68 elements in sludge  samples  collected  during
 1972-1973 from 16 U.S.  cities.  Mercury concentrations were as  follows:
         Atlanta  GA
         Cayuga Heights NY
         Chicago  IL
         Denver CO
         Houston  TX
         Ithaca NY
         Los Angeles CA
         Miami FL
Hg, ppm

   6.9
  10.8
   6.1
   3.6
   3.8
  13.6
   7.1
  15.5
Milwaukee WI
New York NY
Philadelphia PA
San Francisco CA
Schnectady NY
Seattle WA
Syracuse NY
Washington DC
Hg, ppm

   3.4
  15.0
   4.7
  18.0
   9.1
   8.2
   6.4
   5.8
      The mean concentration of these analyses  is  8.5 ppm; and  the median  is
7.0 ppm, in good agreement with Salotto's mean  and median values.  The
extreme variability evident in concentrations of such heavy metals as
cadmium was absent for mercury.  The highest value was only five times as
great as the lowest, whereas for lead and cadmium  the ratio ranged from
55 to 65.

      Very few data have been published which permit estimation of trends  in
mercury levels in sludges.  One piece of evidence  comes  from Chicago, which
since 1969 has had an Industrial Waste Ordinance which has limited the levels
                                   2-25

-------
  TABLE 2.10.   MERCURY IN DRINKING WATER SUPPLIES
State
Alabama
Alaska
Arizona
Arkansas
California
Colorado
Connecticut
Delaware
Florida
Georgia
Idaho
Illinois
Indiana
Iowa
Kansas
Kentucky
Louisiana
Maine
Maryland
Massachusetts
Michigan
Minnesota
Mississippi
Missouri
Montana
Nebraska
New Jersey
New Mexico
New York
North Dakota
Ohio
Oklahoma
Oregon
Ponnsy 1 van i a
Rhode Island
South Carolina
South Dakota
Tennessee
Texas
Utah
Vermont
Virginia
Washington
West Virginia
Wisconsin
Wyoming
Guam
Puerto Rico
Total
Number of
Analyses,
:0.0005 ppm

16
3
1
7
it
1
36
1
1
1
7
9
11
4
4
2
13
22
30
8
1
1
3
14
3
2
1
83
16
15
1
1
23
1
3
57
3
2
11
2
5
3
7
4
4
13

460
Number of
Analyses,
>0.005 ppm
1
1


3


12
3
1





1

3

1





1


12
2





1
3
1

1
1
1
1


1

1
52
Total
Number of
Analyses
1
17
3
1
10
4
1
48
4
2
1
7
9
11
4
5
2
16
22
31
8
1
1
3
14
4
2
1
95
18
15
1
1
23
1
4
60
4
2
12
3
6
4
7
4
5
13
1
512
Maximum
Concentration,
ppm
0.0030
0.0005
<0.0005
<0.0005
0.0010
< 0.0005
<0.0005
0.0010
0.0010
0.0025
<0.0005
<0.0005
<0.0005
<0.0005
<0.0005
0.0010
<0.0005
0.0013
<0.0005
0.0005
<0.0005
<0.0005
<0.0005
< 0.0005
<0.0005
0.0010
<0.0005
<0.0005
0.0010
0.0010
<0.0005
<0.0005
<0.0005
<0.0005

-------
      Since  rocks  are  the  precursors  of  soils, mercury  content  in  soils
undisturbed  by man would be  expected  to  approximate  those  found in rocks.
This  appears to  be the case,  as  evidenced by  results  of U.S. soil  studies
conducted  by the U.S.  Geological Survey  (Shacklette  et  al., 1971).   Samples
 (912) of soils and other regoliths  (loose surface  rocks) were collected
 from  sites approximately 59  miles apart  throughout the  United States.  The
 geometric  mean of  all  samples was 0.071  ppm (0.112 ppm  arithmetic  mean).
 The geometric mean for the United States east of the 97th  meridian was
 0.096 ppm  (0.147 ppm arithmetic  mean); the  geometric mean  for the  western
 United  States was  0.055 ppm  (0.083  ppm arithmetic  mean).   Of all locations
 sampled, 67  percent  contained less  than  0.080 ppm, and  only 16  percent
 exceeded 0.175 ppm.

      In a similar survey  covering  29 eastern states, Wiersma and  Tai  (1974)
 found no significant difference  between  mercury concentrations  in  cropland
 and noncropland.  Geometric  means were 0.063  and 0.54 ppm, and  arithmetic
means were 0.08  and  0.07 ppm, respectively.   These average values  were not
 significantly different from the averages found by Shacklette et al. for
 western states.

      Additional data  on mercury concentrations in U.S. soils are  available
 from  the Environmental Protection Agency's  1972 National Soils  Monitoring
 Program.   Results  of mercury  analyses are shown in Table 2.12 (Gowen et al.,
 1973).  In each  Standard Metropolitan Statistical  Area  (SMSA) except
 Fitchburg, Massachusetts,  urban  area  soils  had significantly higher mercury
concentrations than  the corresponding suburban area  soils.  A number of the
values  for suburban  soils  appeared  to be exceptionally  low in light of the
U.S.  soil  averages determined by Shacklette et al. (1971) and Wiersma and
Tai (1974).   Closer  agreement was exhibited by the results of a similar
National Soils Monitoring  Program the following year at five different
cities  (U.S.  Environmental Protection Agency, 1974a), which are also
summarized in Table  2.12.  As in the  previous study, mercury levels in urban
soils were found to  be significantly  higher than those  in suburban soils;
average suburban soil  concentrations  (geometric means)  ranged from 0.08 to
0.17 ppm,  consistent with  Shacklette  and Wiersma and Tai.  The  Tacoma,
Washington,  results  may be atypical since this is  the site of a large copper
smelter noted for  the  variety of feed materials which it processes.

      One of  the principal noncrop uses  of mercurial fungicides is on grass
of the greens at golf  courses which are  particularly susceptible to fungus
diseases.   Thus, mercury content  of golf-course soils can be elevated, as
evidenced by the findings of  an  unpublished EPA study of 94 golf courses in
the northern and central United  States (U.S. Environmental Protection Agency,
1974b).   The arithmetic mean  for  greens  and fairway cores were  as  follows:

                                  Mercury Concentration, ppm
               Soil Core Depth        Greens    Fairways

                 0.0-10.1 cm           72.7        6.8
                10.1-20.3 cm           30.5        0.97
                20.3-30.5 cm           14.1        0.52
                                    2-29

-------
          TABLE 2.12.  CONCENTRATIONS OF MERCURY IN URBAN
                       AND SUBURBAN SOILS
Mercury Concentration, ppm
Number of
Location Samples
Washington DCa
Urban
Suburban
o
Evansville IN
Urban
Suburban
Pittsfield MAa
Urban
Suburban
Greenville SC3
Urban
Suburban
o
Tacoma WA
Urban
Suburban
Des Moines I A
Urban
Suburban
Fitchburg MA
Urban
Suburban
Lake Charles LA
Urban
Suburban
Pittsburgh PAb
Urban
Suburban
Reading PAb
Urban
Suburban

52
64

34
30

40
5

24
62

47
48

59
25

26
10

16
54

51
138

10
41
Arithmetic
Mean

0.48
0.16

0.27
0.09

0.33
0.17

0.17
0.10

0.76
0.19

0.44
0.01

0.34
0.24

0.06
0.01

0.64
0.09

0.63
0.10
Geometric
Mean Range

0.25
0.13

0.16
0.08

0.25
0.15

0.12
0.09

0.42
0.17

0.103
0.004

0.290
0.167

0.020
0.002

0.459
0.044

0.292
0.080

0.07-7.81
0.03-1.12

0.04-3.55
0.04-0.25

0.11-2.51
0.07-0.27

0.06-1.19
0.05-0.27

0.08-7.90
0.10-0.54

0.00-15.39
0.00-00.15

0.08-00.83
0.00-00.50

0.00-00.29
0.00-00.18

0.11-02.09
0.00-00.74

0.04-02.84
0.00-00.44
Positive
Detection,
%
















89
16

100
90

50
7

100
68

100
93
 Source:  U.S. Environmental  Protection Agency, 1974a, 1973 data.

'Source:  Gowen  et al., 1973,  1972  data.
                                   2-30

-------
 The maximum value found (0 to 10 cm sample)  was 338 ppm.   Since neither
 greens nor fairways are tilled,  the above-background mercury concentrations
 at depths of up to 30 cm are evidence of migration of applied mercurial
 fungicides.
 TERRESTRIAL BIOTA

 Vegetation

       Mercury concentrations in tree species of unpolluted areas such as
 Cades Cove, Tennessee, in the Great Smokey Mountains and Lake Powell, Utah,
 average 0.02 to 0.03 ppm (Shacklette, 1970; Huckabee,  1973).   In the  more
 urban areas of Missouri and New Haven, Connecticut,  concentrations  are
 higher, ranging from 0.2 to 1.0 ppm (Shacklette, 1970;  Smith, 1972).   In
 New Haven it is interesting to note that even at 14  km from the  city, mercury
 concentrations are similar to those in the city.  In studies  of  vegetation in
 the vicinity of a New Haven oil-fired power plant, mercury concentrations  did
 not vary significantly with distance and in general  were similar to levels
 found throughout the city (Mondano and Smith, 1974).  Ohio levels were found
 up to 0.03 ppm, similar to Cades Cove and Lake Powell.   In contrast,  trees
 and shrubs at Red Devil, Alaska, growing in soil over a cinnabar (HgS) vein,
 contain almost a hundred times more mercury, 1.0 to  1.25 ppm  (Shacklette,
 1970).  Mercury levels vary between leaves and twigs,  however, with no
 consistent trend.  In some instances levels are higher in leaves, in  others,
 stems.

       As was the case for trees and shrubs, mercury  levels found in herba-
 ceous growth in unpolluted areas generally range from 0.02 to 0.03  ppm.  Ohio,
 Utah, and Nevada have these levels (Devendorf, 1975; Standiford  et  al.,  1973;
 Gay,  1976).   The effect of air pollution from a copper smelter is evident  in
 the data from Tacoma, Washington,  where mercury concentrations in garden
 vegetables  ranges from 1.1 to 4.0  ppm within 3.2 km  of the facility (Ratsch,
 1974).  Levels do decrease with distance from this facility.  However, even
 at  9.7 km,  levels are ten times higher than those in unpolluted  areas.

       Mercury concentrations found in grasses do not exceed the  0.02  to  0.03
 ppm found in trees,  shrubs,  and herbs under unpolluted conditions.  Even
 samples near a stack emitting fly  ash in Oak Ridge,  Tennessee, contained only
 0.025 ppm (Huckabee,  1973).

       Mosses and lichens have a tendency to accumulate more mercury than
 trees,  shrubs,  grasses,  or herbs,  even in unpolluted areas.   For example,  at
 Cades Cove,  Tennessee,  mercury concentration in trees,  shrubs, herbs, and
 grasses  averaged 0.02 to 0.03 ppm,  whereas levels in mosses ranged  from
 0.092 to  0.118  ppm (Huckabee,  1973).   Levels in mosses  and lichens  in New
 Haven,  Connecticut,  are  again higher than they were  in other  plant  types,
 averaging over  1.0 ppm (Mondano and Smith,  1974).  Mosses and lichens in
 all other sample areas  except Oak  Ridge,  Tennessee,  1.18 ppm  (Huckabee,
 1973),  contained levels  similar to  those found at Cades Cove.  Some variation
 does  exist,  however,  the tendency  is toward higher concentrations in  areas of
higher population  and industry (Yeaple,  1972).

                                      2-31

-------
 Mammals

       Levels  of mercury  found  in mammals vary  among regions of the U.S.,
 among  species, within  species, and with age.   There are  no national sampling
 programs  for  mammals and few local studies have  emphasized this group of
 animals.   Cumbie  (1975a,b) and Cumbie and Jenkins  (1974)  have shown that
 mercury levels in mammals vary among physiographic  provinces in the Southeast
 (Table 2.13).  Body burdens increase toward the  coast, with levels being
 highest in the Lower Coastal Plain, intermediate in the  Upper Coastal Plain,
 and  lowest in the Piedmont.  This has also been  shown to  occur with other
 metals (Cumbie 1975a)  and may be a result of different levels of soil
 mineralization in the  provinces.

       Lynch (1973) indicates that squirrels in rural areas may accumulate
 higher mercury levels  than those in urban environments;  this is thought  to
 be related to the ingestion of seeds treated with mercurial fungicides.
 Levels may also vary among species; herbivorous  species  such as deer and
 livestock tend to have lower levels than omnivorous and  carnivorous species
 such as fox and mink.  Carnivores which feed at  least in  part on aquatic
 organisms tend to accumulate the highest levels  of  mercury.   Bioaccumulation
 is also illustrated by the fact that adults tend to have  higher levels than
 young  animals.  Mercury  concentrates at different rates  in different parts
 of the body.  Concentration values tend to be  highest in  hair (dry weight
 basis), intermediate in  liver  (wet weight), and  lowest in muscle,  fat, and
 milk (wet weight).  On a dry weight basis, mercury  levels are probably
 greatest  in the liver  (Cumbie, 1975a).
 Birds

      Mercury occurs in birds in all parts of the U.S.  The amounts vary
 among years, seasons, regions, and bird species.  A nationwide monitoring
 program in starlings revealed declining mercury levels, which were
 attributed to the decline in the use of pesticides and more stringent
 discharge requirements (Table 2.14).  No differences were detected between
 urban and rural environments.

      A national survey of mercury in woodcocks illustrates that mercury
 levels in birds may vary in different regions of the U.S.  (Clark and McLane,
 1974) (Table 2.15).  Woodcock in the southern U.S. contained more mercury
 than those in the North; this was presumed to reflect greater ambient levels
 of mercury in the South.  A national study of mercury levels in duck wings
 also demonstrates regional differences (Heath and Hill, 1974).  Ducks collec-
 ted in the Atlantic and Pacific flyways had greater levels of mercury than
 those from the Mississippi and central flyways (Table 2.16).

      In addition, Heath and Hill (1974) show that differences in concen-
 trations may occur in similar species; black duck levels were about twice as
high as mallard levels.  This is probably related to the fact that black
ducks are more carnivorous and thus higher on the food chain.  Species
differences are illustrated when diving and sea ducks were shown to have
considerably higher mercury levels than dabbling (pubble) ducks (Table 2.17)


                                   2-32

-------
                              TABLE  2.13.   MERCURY LEVELS IN TERRESTRIAL MAMMAL HAIR
10
I
U)
Location
Florida
Statewide
Northern
Southern
Alachua County
Dade County
Eglin Air Force Base
Georgia
Piedmont
Upper Coastal Plain
and lower coastal
plain
Lower Coastal Plain
Concentration ,
ppm wet weight
Organism
Raccoon, Procyon lotor, adult
Raccoon, P. lotor, juvenile
Raccoon, P. lotor
Raccoon, P. lotor, adult
Raccoon, P. lotor, -juvenile
Raccoon, P. lotor, adult
Raccoon, P. lotor, juvenile
White-tailed deer, Odocoileus
virginianus
Bobcat , Lynx ruf us
Gray fox, Urocyon cinereoargenteus
Mink, Mustela vison
Opossum, Didelphis virginiana
Otter, Lontra canadensis
Raccoon, P. lotor
White-tailed deer, 0. virginianus
Bobcat, L. ruf us
Raccoon, P. lotor
Bobcat, L. ruf us
Gray fox, U. cinereoargenteus
Mink, M. vison
Opossum, D. virginiana
Otter, L. canadensis
Raccoon, P. lotor
Red fox, Vulpes vulpes
White-tailed deer, 0. virginianus
Mean
6.52a
2.65a
7.50a
4.35a
10. la
2.65a
6.38a
40. Ia
4.4a
NDa
2.39a
0.50a
10. 7a
1.30a
15. 9a
0.13
13.05a
7.36
0.93a
0.763
10. 7a
2.39a
37.6aa
6.12
0.49a
Range
0.52-35.7
0.84-10.1
0.13-0.4a
0.39-23.4
ND-1.06
2.3-17.3
0.39-3.31
9.3-26.8
ND-0.59a
1.14-24.0
0.31-15.2
0.22-2.31
0.82-8.41
5.9-15.4
0.39-23.4
15.8-67.9
0.23-50.6
0.19-0.26
ND -0.21a
Reference
Cumbie, 1975a
Cumbie & Jenkins ,
Cumbie, 1975a
Cumbie & Jenkins ,
Cumbie, 1975a.
Cumbie & Jenkins ,
Cumbie, 1975b
Cumbie & Jenkins,
Cumbie, 1975b
Cumbie, 1975a
Cumbie, 1975b
Cumbie & Jenkins,
Cumbie, 1975b
Cumbie & Jenkins,

1974
1974
1974
1974
1974
1974

-------
                                                  TABLE  2.13.   (Continued)
        Location
Organism
                                                                  Concentration,
                                                                  ppm wet weight
Mean
Range
Reference
     Piedmont
       Upper Coastal Plain  Opossum,  I),  virginiana              0.28a    0.16-0.38
                             Raccoon,  £.  lotor                   1.553    0.28-3.81
                             Red fox,  V.  vulpes                  2.34     0.06-5.67
                             White-tailed deer, 0.  virginianus   0.55S    0.24-0.98
    aValues  in  ppm dry weight.

    bAll  data collected 1972-1973.
I
OJ
.C-

-------
TABLE 2.14.  MERCURY LEVELS IN STARLINGS (STURNUS VULGARIS); 1970, 1971, AND 1973a
Concentration, ppm wet weight^
Location
Alabama
Marion
Mobile
Tallaega
Arizona
Graham
Maricopa
Nava j o
Phoenix
Yavapi

Arkansas
Lonoke/Pulaski
K> Stuttgart
w Yell/Pope
Ln
California
Bakersfield
Colusa
Imperial
Inyo
Kern
Los Angeles
Mo doc
Monterey
Sacramento
Shasta
Stanislaus
Ventura
Colorado
Adams
Brighton
Greeley
1970 1971 1973

<0.05
0.07 0.07
<0.05

<0.05
<0.05
<0.05
0.01 <0.01
<0.05


0.05
0.06 0.03
<0.05

0.05 <0.01
0.05
0.09
0.08
<0.08
0.01 <0.01
0.07
<0.05
0.04 0.06
<0.05
0.07
<0.05

<0.05
<0.01
0.02
Concentration, ppm wet weightb
Location
Colorado
La Plata and
Rio Grande
Montrose
Otero
Connecticut
Connecticut River
Valley
New London

Delaware
Dover

Florida
Bay
Gainesville
Hardee
Madison
Polk

Georgia
Atlanta
Pike
Wayne
Idaho
Boise
Franklin
Minidoka
Nezperce
Owyhee
Illinois
Chicago
Cook
1970

<0.05

0.05
<0.05



0.05





<0.05
<0.05
<0.05
<0.05



<0.05
<0.05


<0.05
0.06
0.05
0.05


0.07
1971 1973






0.03 <0.01




0.03


0.21 <0.01





0.03 0.01


0.07 <0.01





0.01 <0.01


-------
TABLE 2.14.  (Continued)
Concentration, ppm wet weight
Location
Illinois (cont'd)
Sagamon
Stephenson
Indiana
Evansville
Gary
Henry
Highland
Iowa
Butler
Polk
Pottawattomie
Des Moines

Kansas
Garden City
Hamiltion & Kearny
Marion
Nemaha
Rowl ins
Smith

Kentucky
Hopkins
Ohio

Louisiana
Baton Rouge
Jefferson
Rapides
Maine
Gray
Penobscot
1970 1971 1973

<0.05
<0.05

<0.01
0.01
0.19
0.02

<0.05
<0.05
<0.05
Not de- <0.01
tected

<0.01 0.01
0.15
0.15
0.18
0.10
0.07


0.10
<0.05


0.05 0.02
<0.05
0.10

0.06 <0.01
0.05
Concentration, ppm wet weight
Location
Maryland
Annapolis
Patuxent
Prince George
Massachusetts
Quincy
Michigan
Chippewa
Grand Traverse
Inaham
Kent
Lansing

Minnesota
Aitkin
Pine
Swift
Twin Cities

Mississippi
Harrison
Jackson
Leake
Starkville

Missouri
Bellinger
Maiden
Stoddard
Montana
Meagher
Missoula

1970 1971


<0.01
0.10

0.05
0.05
0.06
0.05
0.05
0.03


<0.05
<0.05
<0.05
0.01


<0.05
0.08
<0.05
0.01


<0.05
0.02
0.05

0.06
<0.05

1973

<0.01
<0.01


<0.01




<0.01





<0.01





<0.01



0.01






-------
TABLE 2.14.  (Continued)
Concentration, ppm wet weight^
Location
Montana (cont ' d)
Richland
Yellowstone

Nebraska
Antelope
Clay
Keith
Lincoln
North Platte

Nevada
N> Clark
u> Humboldt
^ McGill
Nye
Reno

White Pine
New Jersey
New Brunswick
New Mexico
Bernalillo
Carlsbad
Chaves
Farmington
Luna
Quay
Santa Fe & Torrence

New York
Albany
Jamestown
Oswego
1970 1971 1973

0.08
<0.05


<0.05
<0.05
0.05
<0.05
0.01 0.01


0.06
0.06
0.05 <0.01
0.09
0.02 0.20

0.11

0.22 <0.01

<0.05
0.05 <0.01
<0.05
0.025 <0.01
0.08
<0.05
<0.05


<0.01
0.03 <0.01
0.06
Location
New York
Rennselaer

North Carolina
Macon
Pender
Raleigh
Union
Wilkes

North Dakota
Bismarck
Cavelier
Dickey
Manden
Ward

(Thi n
l/Ll-LU
Columbus
Erie
Jefferson
Washington
Oklahoma
Canadian
Greer
Nowata
Okmulgee
Tishomingo

Oregon
Baker
Corvallis
Harney
Klamath
Concentration, ppm wet weight"
1970

0.10


<0.05
<0.05

<0.05
0.05



0.11
<0.05

0.05



0.11
<0.05
0.08

0.13
0.08
0.13
0.09



0.14

0.10
0.11
1971 1973






0.01 <0.01




0.01


0.02



0.06 0.06








0.01 <0.01



0.32 0.05



-------
                                               TABLE 2.14.  (Continued)
I
w
CD
Concentration, ppm wet weight
Location
Oregon (cont'd)
Lane
Wilsonville
Yamhill

Pennsylvania
Cuzerne
Pittsburgh
Sommerset
South Carolina
Aiken
Columbia
South Dakota
Brown
Hughes
Pierre
Potter
Tennessee
Davidson
Nashville

Texas
Clay
Hillsboro
Morris
San Antonio

TT+- o>i
u tan
Salt Lake City
Sevier/Millard
Weber

1970 1971 1973

1.90
0.62 0.05
1.50


0.10
0.06 <0.01
0.10

<0.05
<0.01

0.05
<0.05
0.05 0.13
<0.05

0.05
0.03 <0.01


0.08
0.04 0.01
<0.05
0.03 <0.01



0.030 0.04
0.08
0.09

Concentration, ppm wet weight
Location
Utah (cont'd)
Sevier/Millard
Weber

Vermont
Add is on
Champlain Valley
Virginia
Amherst
Blacksburg
Caroline
Prince George
Washington
Pierce
Spokane
Whitman
Yakima
West Virginia
Elkins
Wisconsin
Curtiss
Horicon
Portage
Tempeleau
Wyoming
Big Horn

Brook
Goshen
Washakie
Wo r land
1970

0.08
0.09


0.05

0.08

0.18
0.22

0.05
0.07
0.06
0.06



0.05

<0.05

0.05

<0.05
<0.05
0.06

1971 1973






0.10

0.01 0.03




<0.01 <0.01

0.02 0.03

0.01 0.01


0.03
0.03





0.01
    ^Sources: For  1970 - Martin  (1972);  1971  - Martin

     Each value  represents  analysis  of  a composite  of
and Nickerson (1973); 1973 - White et al. (1976).

10 starlings.

-------
     TABLE 2.15.  MERCURY LEVELS IN WOODCOCK
                   (PHILOHELA MINOR) LIVER, 1970-1971


Location
Alabama
Arkansas
Florida
Georgia
Kentucky
Louisiana
Maine
Maryland
Michigan
Minnesota
Mississippi
Missouri
New Hampshire
New Jersey
New York
North Carolina
Pennsylvania
Rhode Island
South Carolina
Tennessee
Vermont
West Virginia
Wisconsin

Number of
Samples
5
5
4
5
5
9
5
5
5
5
10
4
4
9
5
5
5
4
5
4
4
5
5

Concentratiot
Mean
0.61
0.17
0.28
0.17
0.09
0.25
0.07
0.22
0.09
0.06
0.17
0.13
0.09
0.19
0.15
0.37
0.10
0.13
0.57
0.35
0.09
0.08
0.10

i, ppm wet weight
Range
0.29-0.92
0.06-0.26
0.18-0.36
0.05-0.23
0.05-0.14
0.13-0.36
0.05-0.12
0.14-0.28
0.05-0.12
0.05-0.08
0.08-0.27
0.05-0.17
0.07-0.12
0.08-0.46
0.08-0.23
0.05-0.87
0.07-0.17
0.05-0.22
0.17-1.10
0.20-0.66
0.07-0.12
0.05-0.14
0.05-0.15
Source:  Clark and McLane, 1974.
                           2-39

-------
TABLE 2.16.  MERCURY LEVELS IN BLACK DUCK AND MALLARD WINGS
             (AMAR RUBRIPES AND A. PLATYHYNCHER),  1969-1970

Location
Atlantic Flyway
Connecticut
Delaware
Florida and Georgia
Maine
Maryland

Massachusetts
New Hampshire
New Jersey

New York

North Carolina
Pennsylvania

Rhode Island
South Carolina

Vermont
Virginia

Mississippi Flyway
Alabama
Arkansas
Indiana
Illinois
Iowa
Kentucky
Louisiana
Michigan
Minnesota
Mississippi
Missouri
Ohio
Tennessee
Wisconsin
Central Flyway
Colorado
Kansas
Organism

Black duck
Black duck
Mallard
Black duck
Black duck
Mallard
Black duck
Black duck
Black duck
Mallard
Black duck
Mallard
Black duck
Black duck
Mallard
Black duck
Black duck
Mallard
Black duck
Black duck
Mallard

Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard

Mallard
Mallard
Number of
Samples

3
2
2
3
3
2
4
2
5
3
4
3
2
3
3
3
2
3
2
3
2

1
6
1
6
4
1
5
3
5
2
5
3
4
5

4
6
Concentration, ppm wet weight
Mean

0.21
0.20
0.12
0.19
0.11
0.08
0.22
0.20
0.18
0.11
0.33
0.11
0.15
0.16
0.08
0.19
0.18
0.13
0.26
0.15
0.16

0.06
0.08
0.06
0.06
0.08
0.09
0.08
0.11
0.08
0.08
0.07
0.10
0.08
0.26

0.08
0.06
Range

0.14-0.24
0.15-0.25
0.09-0.18
0.16-0.24
0.10-0.13
0.08-0.08
0.21-0.22
0.20-0.20
0.15-0.23
0.10-0.12
0.18-0.80
0.09-0.12
0.14-0.16
0.12-0.19
0.06-0.10
0.14-0.22
0.18-0.19
0.11-0.15
0.14-0.39
0.13-0.17
0.15-0.16


0.06-0.10

0.05-0.08
0.06-0.10

0.06-0.09
0.08-0.14
0.06-0.10
0.07-0.08
0.05-0.11
0.08-0.12
0.07-0.12
0.05-1.00

0.07-0.10
Trace-0.09
                            2-40

-------
                          TABLE 2.16.  (Continued)
Location
Central Flyway
Montana
Nebraska
New Mexico
North Dakota
Oklahoma
South Dakota
Texas
Wyoming
Pacific Flyway
Arizona and
New Mexico
California
Colorado
Idaho
Montana
Nevada
Oregon
Utah
Washington
Wyoming
Organism
(cont'd)
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard


Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Mallard
Number of
Samples

4
7
3
6
4
6
6
3


3
8
3
8
4
3
7
5
8
2
Concentration
Mean

0.07
0.09
0.09
0.08
0.08
0.06
0.06
0.08


0.12
0.08
0.10
0.10
0.10
0.37
0.09
0.09
0.09
0.12
, ppm wet weight
Range

0.05-0.09
0.07-0.13
0.06-0.10
0.06-0.11
0.05-0.11
0.05-0.09
0.05-0.07
0.07-0.09


0.09-0.14
0.05-0.11
0.09-0.12
0.08-0.15
0.10-0.11
0.23-0.55
0.06-0.10
0.06-0.10
0.05-0.18
0.10-0.13
Source: Heath and Hill, 1974.
                                        2-41

-------
     TABLE 2.17.  MERCURY  LEVEL IN DUCK BREAST MUSCLE, 1970-1971
Location
Location
Alabama
Baldwin County

Mobile County

California
San Francisco Bay



Tule Lake NWR

Maryland
Chesapeake Bay


West River
Minnesota
A nas six
Jtasra
Nortli Carolina
Coro 1 1 a
Craudy
Pamlico Pt.
North Dakota
Audubon NWR
Pettibone
Stan ton
Woodworth

South Dakota
MacPhearson

Marshall County
Sandlake NWR
Texas
Port Lavaca

Vermont
Lake Champlain

Ulah
Brigham City



Organism
Gadwall, Anas strepera
Lesser scaup, Aythya affinis
Gadwall, Anas strepera
Lesser scaup, Aythya affinis
Greater scaup, Aythya marila
Lesser scaup, A. affinis
Mallard, Anas platyrhynches
Pintail, A. acuta
Lesser scaup, Aythya affinis
Mallard, Anas platyrhynches
Canvasback, Aythya valisineria
Common scoter, Melanitta nigra
Mallard, Anas platyrhynches
Surf scoter, Melanitta perspicillato
White-winged scoter, M. deglandi
Canvasback, Aythya valisineria
Mallard, Anas platyrhynches
Lesser scaup, Aythya affinis
Lesser scaup, A. affinis
Mallard, Anas platyrhynches
Mallard, A. pla ty rliyncliL's
Lessor scaup, AyLhya affinis
Mallard, Anas platyrhynches
Mallard, A. plalyrliynchc.s
Mallard, A. platyrhynches
Lesser scaup, Aythya affinis
Mallard, Anas platyrhynches
Lesser scaup, Aythya affinis
Mallard, Anas platrhynches
Lesser scaup, Aythya affinis
Mallard, Anas platrhynches
Lesser scaup, Aythya affinis
Mottled duck, Anas fulvigula
Black duck/Mallard, Anas rubripes/
A. platyrhynches
Common goldeneye, Bucephala clangula
Creater scaup, Aythya marila
Lesser scaup, A. affinis
Mallard, Anas platyrhynches
RiiiK-neckcd duck, Aythya collaris
No. of
Samples
4
9
12
7
8
4
16
5
16
16
8
3
8
1
4
8
16
8
7
8
8
8
8
7
6
16
8
6
8
8
8
16
16

6
8
1
2
16
3
Concentration,
Mean
0.06
0.21
0.89
0.48
0.38
0.27
0.16
0.09
0.37
0.14
0.14
0.12
0.03
0.17
0.30
0.10
0.11
0.16
0.28
0.07
0.03
0.21
0.04
0.09
0.02
0.31
0.03
0.16
0.03
0.31
0.03
0.28
0.26

0.11
0.42
0.02
0.13
0.04
0.1 1
ppm wet weight
Range
0.02-0.09
0.12-0.34
0.08-3.90
0.14-2.00
0.09-0.68
0.03-0.68
0.02-1.06
0.05-0.15
0.02-1.77
0.02-1.47
0.05-0.24
0.06-0.20
0.01-0.06
0.07-0.61
0.08-0.16
0.03-0.24
0.07-0.25
0.10-0.47
0.03-0.12
<0.01-0.16
0.03-0.70
0.01-0.10
0.03-0.18
0.02-0.03
0.04-0.70
0.01-0.05
0.09-0.19
0.02-0.06
0.15-0.65
0.02-0.05
0.06-1.00
0.07-0.43

0.04-0.17
0.21-0.76

0.12-0.14
0.0 1-0. 11)
0.0(i-0. IK
Source.' :  llaskett , 1 975 .
                                      2-42

-------
 (Baskett,  1975).   Dabbling  ducks  are more  herbivorous  and  feed  in shallow
water  or on  the  ground.   Diving and  sea  ducks  are more carnivorous and feed
 on  fish and  botton-dwelling organisms which  bioaccumulate  mercury from the
 water  and  sediment.

       Numerous local  studies of total mercury  levels in various species of
 birds  have been  conducted in the  U.S.  Several studies (Brock et al., 1973;
 Buhler et  al., 1973)  have shown that levels  of mercury in  birds may vary
 with the availability of  mercury-contaminated  foods.   Granivorous birds had
 higher levels at  crop-planting time when mercury-coated seeds were used than
 at  other times of the year.   Birds with  access to mercury-treated seeds may
 have higher  levels than birds from urban or  nonfarm areas  (Lynch, 1973) .
 Some of the  highest levels  reported  in birds have come from Lake St. Clair,
 Michigan,  in recent years;  however,  the  levels reported in bird eggs from
 this area  have declined  (Table 2.18).  The decline  is  probably  related to a
 decrease in  the  amount of mercury discharged into lakes and streams.
 Studies in other  areas needed to  corroborate the decline have not been made.

       Mercury ingested by birds accumulates  at different rates  in different
 parts  of the body (Table  2.19) (Buhler,  1977).  Values tend to  be highest in
 feathers but occasionally are exceeded by  those in  liver tissue.  Liver
 concentrations average from two to six times higher than muscle tissue
 concentrations.   The  mercury background  level  for pheasants raised in
 captivity  in Oregon is 0.001 to 0.002 ppm  in muscle (Buhler, 1977).  On this
 basis, a significant  accumulation of mercury was found in  over  90 percent of
 the Oregon pheasants  collected during 1970.  The mean  concentration of mercury
 in  muscle  tissue  from these birds was 0.173  ppm, with  approximately 7.5 per-
 cent of the  pheasants exceeding the 0.5  ppm  FDA guideline  value for mercury
 in  foods.  The highest concentrations of mercury observed  in this survey
 occurred in  one  bird  from the Willamette Valley that contained  6.33 ppm in
 breast muscle and 12.8 ppm  in the liver.   The  concentration of  mercury in
 Oregon pheasants  varied appreciably among  seasons and  areas, but maximum
 tissue residues  appeared  to be related to  the  time  of  greatest  availability
 of  seeds treated  with mercury fungicides in  a  particular area.

       Almost all  of the mercury found in the muscle tissue from the birds
 collected  in 1970 was in  the form of methylmercury  (Table  2.20), suggesting
 that these birds  had  been exposed primarily  to seeds dressed with methyl-
mercuric dicyanidiamide.  This chemical  has  been widely used in Oregon
 agriculture  and  in 1970 it  accounted for over  80 percent of the mercury
 fungicide  used in the state.
AQUATIC BIOTA

      Levels of environmental mercury appear  to be considerably highest in
the tissues of aquatic biota in Atlantic  coastal  environs—both freshwater
and marine.  The sources of contamination in  this region of  the U.S. are
primarily industrial.  Regionally high values also occur in  the states of
the Pacific Northwest—particularly  in the Columbia and Snake River
drainages in Washington and Idaho.   The elevated  levels result from both
mining activity and the leaching of  soils naturally high in  mercury content.

                                   2-43

-------
                                   TABLE  2.18.   MERCURY LEVELS IN BIRDS OF LAKE ST. CLAIR, MICHIGAN
to
-p-
Organism
Bald eagle, Haliaeetus leucocephalus
American bittern, Botaurus
lentiginosus
Black-crowned night heron,
Nycticorax nycticorax
Black tern, Chlidonias niger
Blue-winged teal, Anas discors
Canada goose, Branta canadensis
Common gallinule, Gallinula
chloropus
Common tern, Sterna hirundo
Dunlin, Calidris alpina
Great blue heron, Ardea herodias
Great egret, Casmerodius albus
Killdeer, Charadrius vociferus
Lesser scaup, Aythya af finis

Organ or
Tissue
Egg
Carcass
Liver
Carcass
Liver
Egg
Carcass
Liver
Breast muscle
Liver
Kidney
Breast muscle
Liver
Kidney
Carcass
Liver
Carcass
Liver
Egg
Carcass
Liver
Carcass
Liver
Carcass
Liver
Carcass
Liver
Breast muscle
Liver
Kidney
Concentration ,
Mean
0.4
0.55
1.75
2.8
14.0
0.77
0.68
2.47
0.68
1.50
1.40
<0.10
0.18
<0.10
0.41
1.4
3.4
18.1
2.72
0.15
0.95
14.4
97.0
0.74
6.3
0.45
2.2
0.81
3.2
2.01
ppm wet weight
Range Reference
Wiemeyer et al., 1972
Dustman et al., 1972
0.046-0.11
0.41-1.3
1.3-2.6
0.10-2.3
0.35-5.0
0.27-4.4
0.4-7.5 Dustman et al, 1972
2.1-39.0
0.63-6.25
<0. 10-0. 20
0.70-1.3
5.3-23.0
14.6-136.0
0.14-0.68
0.5-2.3
0.54-1.2
1.8-3.4
0.96-2.3

-------
                                                     TABLE 2.18.  (Continued)
t-o
I
Organism
Mallard, Anas platyrhynchos
Mallard, A. platyrhynchos
Ring-billed gull, Larus delawarensis
Scaup, Aythya spp.
Spotted sandpiper, Actitis macularia
Black-crowned night heron,
Nycticorax nycticorax
Common tern, Sterna hirundo
Great egret, Casmerodias albus
Mallard, A. platyrhynchos
Organ or
Tissue
Breast muscle
Liver
Kidney
Egg
Liver
Carcass
Liver
Liver
Carcass
Liver
Egg
Egg
Egg
Egg
Concentration, ppm wet weight
Mean
0.48
1.62
1.13
0.82
0.54 ± 0.83
0.41
1.22
0.99 ± 1.00
0.55
2.8
0.45
1.15
0.35
0.077
Range
<0. 10-1. 15
0.23-4.8
<0.10-3.5
0.22-2.7
0.14-0.70
0.65-1.8
0.20-0.76
0.69-2.16
0.21-0.45
<0. 05-0. 26
Reference
Dustman et al., 1972
Ford and Prince, 1975
Dustman et al., 1972
Ford and Prince, 1975
Dustman et al., 1972
Stendell et al., 1976

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            TABLE 2.19.   DISTRIBUTION OF MERCURY IN TISSUES
                         OF OREGON PHEASANTS
                                    Total  Mercury,  ppm
          Tissue
                 Male Pheasant
                    Female Pheasant
Feathers
Liver
Kidney
Crop and contents
Muscle
Gizzard
Skin
Brain
Heart
Testes
Femur
Gizzard contents
Fat
26.0
12.8
6.93
6.57
6.33
5.35
5.00
4.70
4.35
2.99
1.11
0.93

0.36
2.30
3.46
1.67
1.14
1.43
0.40
0.95
2.91
—
0.19
0.54
0.10
       Source:   Buhler, 1977.
           TABLE 2.20.  MERCURY AND METHYLMERCURY CONTENT
                        OF PHEASANT BREAST TISSUE
Year   Bird Number
          Total Mercury,
              ppm
             Methylmercury,   Methylmercury,
                  ppm              %
1970



14
23
26
50
1.14
2.11
2.71
0.337
1.3
1.8
2.6
0.35
114
85.3
95.9
103.8
                                           Mean ± S.D.  99.8 ± 12.1
1972
212
215
220
221
0.088
0.911
3.032
0.486
0.096
0.430
0.324
0.024
110.2
 47.2
 27.2
  4.9
                                           Mean ± S.D.  47.4 ± 45.3
Source:  Buhler,  1977.
                                2-46

-------
Other  generally  isolated  incidences  of  aquatic  biota contamination occur  in
many states  of the  U.S.   These levels are most  often associated  with a
point  source of  mercury emission,  such  as sewage  treatment  and chlor-alkali
plants,  or nonpoint source runoff  from  agricultural or  other  use of mercurial
fungicides.

     Mercury compounds enter  aquatic organisms  both indirectly through  the
food web and directly by  extraction  from solution (Jernelb'v and  Lann, 1971;
D'ltri,  1972; Peterson et al., 1973).  From the available literature, it
can be seen  that highest  accumulations  occur in fishes  at the top of the
trophic  structure,  the sport  fishes  and piscivores.  This seems  to be the
major  pathway for mercury accumulation  in predator  fish.  Occasionally,
elevated levels  were found in carp and  suckers; these are due to direct
extraction,  with less than 25 percent of their  total mercury  accumulation
coming through the  food chain (Jernelb'v and Lann, 1971).  Mercury and its
compounds are made  available  through biological methylation by micro-
organisms (Jensen and Jernelb'v,  1969; D'ltri, 1972).
 Fish

     Analysis  of  mercury in fishes  collected  as  part  of  the National
 Pesticides  Monitoring Program (NPMP)  was  initiated  in 1969  (Henderson et al.,
 1972).   Data are  summarized in Tables 2.21  to 2.26  by geographical region.
 Highest  regional  values  were reported from  the Atlantic  coastal  streams
 (Regions 4  and 5).   These concentrations  are  likely due  to  industrial
 discharge rather  than to either agricultural  runoff or leaching  of natural
 minerals.   Elevated  concentrations  in fishes  from certain areas  of the
 Columbia and Snake Rivers in Washington and Idaho reflect contamination
 from both mining  activity and natural occurrence.   On a  regional basis,
 overall  lowest concentrations were  found  in Region  2, Texas, New Mexico,
 Arizona,  and Oklahoma.   On a state  basis, Alaska has  the lowest  amount of
 contamination  to  date, less than 0.1  ppm.   It should  be  noted, however,
 that only two  locations  were sampled  in Alaska and  this  is not an actual
 assessment  for the entire state.

     In  most cases,  NPMP data reveal  highest  mercury  concentrations in the
 muscle tissue  of  the sport fishes,  particularly  largemouth bass, perch,
 catfish,  and walleye.  Relatively low overall levels  found  in Region 6 may
 reflect  the larger proportion of forage fishes sampled.  Elevated concen-
 trations were  found  in the northern squawfish, a large minnow whose range
 is restricted  to  the western states.   This  fish,  like many of the game
 fishes,  is a voracious piscivore.   Occasionally,  elevated concentrations
 were also found in carp  and suckers.   These data indicate that mercury
 concentration  is related to feeding habit,  whether  indirectly through a
 food chain or  directly from sediments.

     A preliminary study from the National  Marine Fisheries Service (1975)
 reported  the levels  of mercury found  in the muscle  tissues of marine fish
 species  (Figure 2.7). Of the 106  species  tested,  only six had more than
50 percent of  the samples  above the FDA 0.05  ppm mercury guideline—blue
marlin, white marlin, gag,  little tunny, black grouper,  and Hawaiian red

                                  2-47

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  TABLE 2.21.  LEVELS OF MERCURY IN FISH FROM REGION 1, NATIONAL  PESTICIDES
               MONITORING PROGRAM, 1969,a 1970,a AND 1972b
Concentration, ppm

Location 1969
Alaska
Chena River 0.07
Kenai River 0.09
Calfornia
Klamath River 0.16
Sacramento River 0.14
San Joaquin River 0.18
Hawaii
Manoa Stream
Waikele Stream
Idaho
Bear River
Salmon River
Snake River 0.26
Nevada
Colorado River
Truckee River 0.37
Oregon
Columbia River 0.56
Rouge River 0.44
Williamette River 0.18
Washingron
Columbia River
Snake River
Ice Harbor
Yakima River 0.19
Mean
1970

0.06
0.14

0.20
0.20
0.16

0.13
0.17

0.23
0.80
0.21

0.07
0.41

0.55
0.27
0.26

0.14
0.61

0.19

1972C

0.09
0.06


0.18
0.04

0.06
0.19

0.11
0.16
0.11

0.03
0.29

0.10
0.11
0.16

0.13
0.10

0.28

1969

0.06-0.09
0.06-0.12

0.12-0.22
0.11-0.19
0.14-0.20






0.08-1.25d


0.22-0.53C

0.15-1.25d
0.16-0.90f
0.13-0.23




0.3-0.9§
Range
1970

0.05-0.06
0.06-0.26

0.12-0.27
0.18-0.22
0.09-0.23

0.09-0.18
0.06-0.43

0.13-0.37
0.29-1.70d
0.08-0.43

0.05-0.09
0.21-0.65°

0.21-1.10d
0.14-0.51f
0.17-0.33

0.07-0.25
0.10-1.20d

0.07-0.27

1972

0.06-0.14
0.01-0.18


0.02-0.32
0.03-0.05

0.03-0.10
0.02-0.46

0.06-0.14
0.08-0.21
0.01-0.26

0.01-0.05
<0. 05-0. 50

0.08-0.23
0.04-0.18
0.04-0.29

0.02-0.47
0.03-0.18

0.03-0.64d
.Source: Henderson et al., 1972.
 Source: Berger, 1974.
 Calculated from author's data.
 Northern squawfish.
 Largemouth bass.
 Brown bullhead.
g
 Largescale sucker and smallmouth bass.
                                   2-48

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  TABLE 2.22.  LEVELS OF MERCURY IN FISH FROM REGION 2,  NATIONAL PESTICIDES
               MONITORING PROGRAM, 1969,a 1970,a AND 1972b


Location 1969
Arizona
Colorado River <0.05
Gila River
New Mexico
Rio Grande
Oklahoma
Arkansas River 0.07
Canadian River
Red River
Verdigris River
Texas
Brazos River
Colorado River
Nueces River
Pecos River
Rio Grande River 0.04

Mean
1970

0.10
0.15

0.33

0.14
0.10
0.18
0.20

0.13
0.16
0.17
0.18
0.09
Concentration ,

1972C 1969

0.11 <0. 05-0. 05
0.25

0.21

0.07 <0. 05-0. 14

0.06
0.09

0.11
0.18
0.03
0.07
0.06 <0. 05-0. 06
ppm
Range
1970

<0. 05-0. 27
0.11-0.22

0.10-0.52d

0.06-0.22
0.06-0.21
0.10-0.24
0.05-0.44

0.05-0.24
0.14-0.19
<0. 05-0. 40
0.05-0.42
<0. 05-0. 17


1972

<0. 05-0. 49
0.10-0.41

0.16-0.28

<0. 05-0. 08
—
<0. 05-0. 12
0.06-0.15

0.06-0.17
0.11-0.46
0.02-0.06
0.01-0.10
0.01-0.11
 Source: Henderson et al., 1972.
 Source: Berger, 1974.
CCalculated from author's data
 Largemouth bass.
                                    2-49

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  TABLE 2.23.  LEVELS OF MERCURY IN FISH FROM REGION 3, NATIONAL  PESTICIDES
               MONITORING PROGRAM, 1969,a 1970,a AND 1972b
                     	Concentration, ppm	
                     	Mean	  	Range	
     Location        1969   1970   1972C     1969         1970         1972


Illinois
  Illinois River     0.10   0.13   0.05   0.07-0.13    0.08-0.21    0.04-0.06
  Ohio River                0.22   0.01                0.11-0.43    0.09-0.21

Indiana
  Wabash River              0.19   0.12                0.15-0.25    0.07-0.16

Iowa
  Mississippi River  0.16   0.20   0.12   0.10-0.25    0.11-0.33    0.08-0.18

Michigan
  Lake Huron         0.07   0.07   0.06  <0.05-0.13    0.05-0.08   <0.05-0.07

Minnesota
  Mississippi River         0.48   0.21                0.21-0.68d   0.09-0.46
  Red River          0.39   0.31   0.53   0.36-0.41    0.13-0.60    0.32-0.75C

Missouri
  Mississippi River         0.14   0.07               <0.05-0.20    0.06-0.10

Ohio
  Ohio River         0.33   0.32   0.17   0.20-0.50    0.07-0.83d   0.06-0.24

Wisconsin
  Lake Michigan      0.18   0.09   0.24   0.09-0.27    0.07-0.09    0.12-0.26
  Lake Superior      0.10   0.17   0.18  <0.05-0.16    0.06-0.29    0.08-0.28
  Wisconsin River           0.37   0.073               0.11-0.60    0.01-0.15
f\
 Source: Henderson et al., 1972

 Source: Berger, 1974.

 Calculated from author's data.

 Catfish.

 Sauger.

                                    2-50

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  TABLE 2.24.
LEVELS OF MERCURY IN FISH FROM REGION 4,  NATIONAL PESTICIDES
MONITORING PROGRAM, 1969,a 1970,a AND 1972b
Concentration, ppm
Location
Alabama
Alabama River
Tombigee River
Arkansas
Arkansas River
White River
Florida
Apalachikola River
St. John's River
St . Lucie Canal
Suwannee River
Georgia
Altamaha River
Savannah River
Louisiana
Mississippi River
Red River
Mississippi
Yazoo River
North Carolina
Cape Fear River
Roanoke River
South Carolina
Cooper River
Pee Dee River
Tennessee
Cumberland River
Mississippi River
Tennessee River

1969


0.46

0.12
0.21

0.16
0.05
0.23



0.60

0.16




0.23
0.11

0.12


0.16


Mean
1970

0.36
0.44

0.14
0.19

0.11
0.18
0.18
0.22

0.28
0.78

0.14
0.18

0.16

0.37
0.14

0.16
0.47

0.10
0.21
0.45

1972°

0.136
0.33

0.13
0.17

0.10
0.06
0.07
0.17

0.31
0.46

0.11
0.15

0.17

0.18
0.10

0.16
0.24

0.17
0.11
0.39

1969


0.36-0.65d

0.08-0.15
0.13-0.27

0.08-0.23
<0. 05-0. 08
0.13-0.34



0.36-1.00d

0.11-0.22




0.17-0.35
0.09-0.13

0.09-0.14


0.09-0.27


Range
1970

<0.05-0.60d
0.15-0.92d

0.09-0.22
0.12-0.35

0.10-0.13
<0. 05-0. 43
0.06-0.28
0.13-0.37

0.11-0.51
0.17-1.80d

<0. 05-0. 30
0.10-0.24

0.10-0.19

0.25-0.60
0.08-0.21

0.05-0.24
0.19-1.00

0.05-0.15
0.14-0.46
0.27-0.67

1972

0.04-0.36
0.01-0.96d

0.06-0.16
0.11-0.27

0.05-0.19
0.03-0.10
0.06-0.16
0.07-0.49

0.11-0.54
0.14-0.67d

0.06-0.16
0.08-0.18

0.15-0.27

0.10-0.40
0.04-0.23

0.01-0.28
0.10-0.36

0.04-0.34
0.05-0.20
0.22-0.78
, Source: Henderson et al., 1972.
 Source: Berger, 1974.
^Calculated from author's data.
 Largemouth bass.
                                     2-51

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  TABLE 2.25.  LEVELS OF MERCURY IN FISH FROM REGION 5, NATIONAL PESTICIDES
               MONITORING PROGRAM, 1969,a 1970,a AND 1972b


Location 1969
Connecticut
Connecticut River 0.36
Maine
Kennebee River
Penobscot River 0.38
Maryland
Potomac River 0.09
Susquehanna River 0.07
Massachusetts
Merrimac River
New Jersey
Delaware River 0.12
Raritan River
New York
Genessee River 0.20
Hudson River 0.20
Lake Ontario 0 .52
St. Lawrence River
Pennsylvania
Allegheny River
Lake Erie 0.13
Vermont
Lake Champlain
Virginia
James River
Kanawha River <0.05

Mean
1970

0.34

0.66
0.48

0.05
0.07

0.25

0.11
0.21

0.24
0.13
0.84
0.27

0.12
0.31

0.41

0.14
0.06
Concentration ,

1972C 1969

0.21 0.13-0.80d

0.63
0.56 0.23-0.48

0.21 0.08-0.10
0.07 0.05-0.09

0.39

0.11 0.05-0.22
0.18

0.08 0.13-0.25
0.14 0.16-0.25
0.32 0.43-0.65
0.14

0.07
0.13 0.10-0.15

0.36

0.07
0.04 <0.05
ppm
Range
1970

0.18-0.51

0.38-1.20°
0.29-0.64

<0. 05-0. 09
0.05-0.10

0.15-0.33

0.07-0.21
0.08-0.29

0.15-0.39
0.07-0.19
0.30-1.30d
0.18-0.39

0.05-0.18
0.15-0.43

0.27-0.49

0.11-0.20
<0. 05-0. 11


1972

0.12-0.32

0.23-0.99f
0.49-0.62

0.06-0.36
0.03-0.10

0.09-0.56

0.02-0.18
0.10-0.28

0.07-0.11
0.12-0.15
0.24-0.47
0.08-0.24

0.04-0.09
0.04-0.16

0.24-0.50

<0. 05-0. 12
0.01-0.06
.Source: Henderson et al., 1972.
 Source: Berger, 1974.
 .Calculated from author's data.
 White perch.
 -Yellow perch.
 Bass.
                                      2-52

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  TABLE 2.26.  LEVELS OF MERCURY IN FISH FROM REGION 6, NATIONAL PESTICIDES
               MONITORING PROGRAM, 1969,a 1970,a AND 1972b


Location 1969
Colorado
Arkansas River
Rio Grande River
Iowa
Des Moines River
Kansas
Kansas River
Missouri
Missouri River
Montana
Big Horn River
Missouri River 0.14
Yellowstone River
Nebraska
Missouri River <0.05
North Platte River
Platte River
South Platte River
North Dakota
Missouri River 0.19
South Dakota
James River
Utah
Green River <0.05
Utah Lake 0.05

Mean
1970
0.06
0.13
0.17
0.18
0.11
0.26
0.19
0.11
0.14
0.11
0.14
0.10
0.17
0.14
0.18
0.04
Concentration,

1972C 1969
0.04
0.09
0.06
0.05
0.08
0.11
0.24 0.13-0.14
0.12
0.11 <0. 05-0. 07
0.21
0.14
0.23
0.11 0.13-0.30
0.16
0.13 <0. 05-0. 10
0.01 <0. 05-0. 06
, ppm
Range
1970
0.05-0.07
<0. 05-0. 28
0.07-0.24
0.08-0.33
<0. 05-0. 15
0.18-0.35
0.13-0.24
0.08-0.15
0.06-0.20
0.09-0.14
0.11-0.24
0.08-0.15
0.11-0.24
0.08-0.22
0.14-0.24
<0. 05-0. 07


1972
0.04-<0.05
0.05-0.14
0.03-0.09
0.02-0.07
0.04-0.13
0.04-0.16
0.10-0.50
0.05-0.17
<0. 05-0. 20
0.16-0.23
0.11-0.20
0.11-0.34
0.02-0.18
0.10-0.22
0.03-0.20
0.01-0.02
 Source: Henderson et al., 1972.
 Source: Berger, 1974.
°Calculated from author's data.
                                    2-53

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Ul
-p-
           7
        0.005-0.017
        0.017-0.481
           0.003-0.582
    0.026-0.457
     (offshore)
  .000-0.016
  000-6.983

.014-0.228
084-0.117
.010-0.398
                                                                           0.005-0.991
                                                                    0.044-0.104
                                                       0.001-1.511
                        0.003-3.573
                 Figure 2.7.   Levels of mercury ±n fishes,  National Marine Fisheries
                              Service Microconstituent Resource Survey (in ppm,.wet
                              weight—range of means)(National  Marine  Fisheries
                              Service, 1975).

-------
snapper.  An additional eight  species were  found  to have mean values  greater
than 0.5 ppm.  Highest concentrations were  found  along  the  southeastern
coast line, particularly  along North Carolina and Florida.  Elevated  values
were also found near  Galveston Bay, Texas;  San  Diego, California; Protection
Island, Washington; and Hawaii.

     Areas of known or suspected  mercury  contamination  have been the  sites
of many localized  investigations  of the mercury levels  in fish  tissue.  The
most heavily studied  include sites of industrial  pollution  such as the
Columbia and Savannah Rivers and  the Great  Lakes  (Buhler et al., 1973;
Buhler, 1977; Roberts et  al.,  1975; Kelso and Frank, 1974;  Pillay et  al.,
1972; Greig and Seagran,  1972) as well as locations where contamination is
due to either natural leaching of soils or  mining activity, such as in the
Columbia and Snake River  systems  and Lake Powell,  Arizona (Blinn et al.,
1974; Buhler et al.,  1973;  Buhler, 1977;  Potter et al., 1975).  Two states,
Ohio and Wisconsin, have  been  extensively surveyed in comprehensive studies
(Stiefel, 1975; Kleinert  and DeGurse, 1972).

     Incidences of excessive contamination  also occurred in Lake St.  Clair,
Michigan (Greig and Seagran, 1972; Bails, 1972),  Lake Erie  (Carr et al.,
1972; Greig and Seagran,  1972; Pillay et  al., 1972; Stiefel, 1975), Lake
Michigan (Kleinert, 1972; Kleinert and DeGurse, 1972),  and  Cranberry  Lake
in New York (Roberts  et al., 1975).  Lowest levels found were in fishes
from the Great Smokey Mountains in North  Carolina, with mean values less
than 0.05 ppm  (Huckabee et  al., 1974; Huckabee, 1972).

     Consistently  elevated  levels of mercury reported in localized marine
fish studies were  found on  the Atlantic coast in  Massachusetts' offshore
waters (Greig et al., 1975) and particularly along the  South Carolina-
Georgia coastal region (Stickney  et al.,  1975;  Reimold  and  Shealy, 1976).
Elevated concentrations were found in other areas  also  but  usually in a
single species, i.e., blue  marlin near Hawaii (Rivers et al., 1972;
Shultz et al., 1976), spiny dogfish off the coast  of Oregon (Childs and
Gaffke, 1973, and  starry  flounder in Puget  Sound,  Washington (Buhler  et al.,
1973).  Sources of contamination  along the  Atlantic coast appear to be
industrial (Roberts et al., 1975), whereas  in Hawaii and Puget  Sound  the
elevated levels are likely  due to natural phenomena (Schultz et al.,  1976;
Buhler, 1977; Buhler  et al., 1973).

     Although there are wide variations in  mercury content  among sampling
locations, the literature indicates that  overall  concentrations of mercury
found in fish tissue  are  slightly higher  in freshwater  fishes.  This  may
be due, in part, to the greater dilution  and dispersion capacity of oceans
and the far-ranging habits  of most large  ocean  species.  There  is some
evidence favoring  these hypotheses in the lower concentrations  found  in
fishes from offshore  north  Atlantic waters  and  in  the Pacific Northwest
(Greig et al., 1975;  Buhler, 1977).

     Fish in reservoirs contain higher levels than those collected upstream
in free-flowing sections of rivers (Gebherds et al., 1971;  Buhler, 1977).
Apparently, deposition of sediments rich  in mercury in  reservoirs provides
                                   2-55

-------
an opportunity for maximum bacterial formation of methylmercury,  which is
then readily accumulated by fish.

     In both the freshwater and marine studies,  there  is much  evidence that
mercury accumulates through the aquatic food chain, resulting  in  highest
concentrations in the muscle tissues of those species  at the top  of  the
trophic structure, the piscivores  (Buhler, 1977; Potter et al., 1975;
Roberts et al., 1975; Pillay et al., 1972; Stiefel, 1975; Walter  et  al.,
1973; Gardner et al., 1975; Peterson et al., 1973).  There are a  few
studies where no indication of bioaccumulation was statistically  detected
(Greig et al., 1975; Knauer and Martin, 1972; Huckabee et al., 1974).
However, the study by Knauer and Martin utilized relatively small sample
sizes and that by Huckabee et al. investigated an area of extremely  low
environmental mercury levels.

     Mercury concentration increases with  fish weight, length, and age
(Kelso and Frank, 1974; Mathis and Kevern, 1973; Knight and Herring, 19725
Richins and Risser, 1975; Peterson et al., 1973; U.S.  Senate, 1971).
Between 61 and 100 percent of the total mercury found  in fishes is present
as methylmercury (Henderson et al., 1972;  Buhler et al., 1973; Huckabee et
al., 1974; Gardner et al., 1975; Knauer and Martin, 1972).  Tests have
confirmed that significant amounts of a single oral dose of methylmercury
is demethylated in sea lions (Buhler and Mate, 1977).  This process  probably
occurs in the liver.  In addition, if inorganic mercury was released at some
other site, it would tend to concentrate primarily in  the kidney  in  the same
manner as it does in animals that have been dosed with inorganic  mercury.
Shellfish

     Highest levels of mercury in shellfish are reported on the Atlantic
coast, particularly near offshore Massachusetts and the Brunswick area of
Georgia  (Roberts et al., 1975).  With the exception of isolated elevated
values in Lavaca Bay, Texas, the lowest values reported in the literature
were found in the Gulf of Mexico (Roberts et al., 1975).  At all locations
crabs appeared to have the greatest accumulations.  This could be due to
their omnivorous food habits.

     Data from the National Marine Fisheries Services Resource Survey
(National Marine Fisheries Service, 1975) report mercury concentrations
in samples of 11 species of mollusks and six species of Crustacea collected
from all coastal U.S. waters.  Only two species, brown shrimp and Dungeness
crab, had mean mercury levels greater than 0.1 ppm.
Marine Mammals

     Highest mercury concentrations reported in marine mammals from a
rather limited data base were found on the Pacific coast—San Miguel
Island, California (Anas, 1974), and the Oregon coast (Buhler et al., 1975),
in seals and sea lions, respectively.

                                   2-56

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     The general literature indicates that various species of marine
mammals were capable of accumulating relatively high tissue concentrations
of mercury  (Buhler et al., 1975).  Seals and walrus which do not migrate
from the Arctic region generally contain relatively low concentrations of
mercury found in some species of marine mammals, such as California sea
lions, is a consequence of the animals living and feeding in U.S. and
Canadian coastal waters.  Mercury concentrations in coastal areas are
known to be higher than those in the open ocean (Jonasson and Boule,
1972; Klein and Goldberg, 1970) as a result of natural and man-related
mercury contamination of the marine environment.

     With the possible exception of the sea lion epizootic in 1970,
carnivorous marine mammals consume large quantities of fish and other
marine organisms containing significant amounts of methylmercury with no
apparent ill effect.  The presence of inorganic mercury in the liver of
California  sea lions and other marine mammals which ingest large amounts
of methylmercury suggests that these animals, and perhaps other species
as well, may have some enzymic or other system for detoxifying methyl-
mercury by  converting it to the inorganic form.  Studies by Lee et al.
(1977) suggest that a low-molecular-weight protein called methallothionein
may play a protective role in the animals by binding significant amounts
of the mercury present in tissues.

     There  is some evidence that mercury is accumulated as much as 500+
times the concentration of seawater by organisms inhabiting the sediment-
water interface (Klein and Goldberg, 1970) , and that mercury is transferred
through benthic food chains by much smaller factors (Williams and Weiss,
1973).  However, a study by Stickney et al. (1975) showed no positive
correlation between the levels found in invertebrates and those found in
predator fishes.

     In order to determine or estimate the environmental distribution of
mercury in aquatic invertebrates in the U.S., studies utilizing similar
organisms and tissues of those specimens from many more locations are
necessary.
Aquatic Plants and Plankton

     Few studies have been conducted on the levels of mercury in aquatic
plants and plankton.  The data from a study of Lake Powell, Arizona
(Table. 2.27) may be representative of areas of high natural mercury levels
in water (Standiford et al., 1973; Potter et al., 1975).  These data show
higher levels of mercury in submerged aquatic plants than in algae, moss,
or periphyton.

     A study of salt-marsh grass on the Georgia coast showed that roots
of the grasses accumulate levels of mercury as much as an order of magnitude
greater than those accumulated by other plant parts (Windom, 1973) .  Levels
found in phytoplankton ofter appear slightly greater than those in zoo-
plankton (Copeland, 1972; Knauer and Martin, 1972).  An investigation by


                                   2-57

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 Knauer and Martin (1972) measured the methylmercury fractions of wet weight
 samples of phytoplankton.  Between 12 and 67 percent of the total mercury
 content was methylmercury.
                  TABLE 2.27.  LEVELS OF MERCURY IN PLANTS
                               OF LAKE POWELL, ARIZONA
       Organism
   Organ
Concentration, ppm wet weight
  Mean           Range
Nonvascular plants
Algae
Algae, lichen soil crust
Moss
Periphyton, shallow water
Vascular plants
Cattail, Typha latifolia

Green tissue
Green tissue
Green tissue
Green tissue

Leaf and green

0.015
0.023
0.016
0.032

0.036

0.008-0.021
0.014-0.022
0.012-0.020
0.021-0.042

0.025-0.047
  Joint-fir, Ephedra sp.

  Juniper, Juniperus
    osteosperma

  Snakeweed, Gutierrezia
    microcephala
  Unidentified grass
  Unidentified shrub
  tissue
Leaf and green     0.025
  tissue
Leaf and green     0.018
  tissue
Stem               0.021
Stem               0.090

Leaf               0.015
Stem               0.039
              0.000-0.040

              0.019-0.023
              0.076-0.104

              0.010-0.020
Source:   Standiford et al.,  1973.
                                    2-58

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                       3.  BEHAVIOR  IN THE ENVIRONMENT
      Mercury enters the environment  through vaporization, volatilization,
weathering, dissolution, precipitation, and biodegradation.  Its environ-
mental movement depends on  its oxidation  state;  the redox potential,
temperature, and pH of the  environment; solubility; volatility; and the
nature of available chemical species  with which  it forms strong bonds
(Krenkel, 1974).  Transport and  transformation data indicate that all com-
ponents of the biosphere contain traces of mercury and constitute potential
sources of exposure for all biota, including man.

      Some of the complexities of the behavior of mercury in the environ-
ment include the following  characteristics: (1)  forms and compounds of mer-
cury in nature, (2) transformation of mercury in nature, (3) the mercury
geochemical cycle, and (4)  transport  of mercury  in the food chain.  Emphasis
in this discussion is on those characteristics that influence its persistence
and environmental fate.
TRANSFORMATION IN NATURE

      Although mercury exists in nature in its elemental Hg(0), mercurous
Hg(I), and mercuric HG(II) oxidation states, it is readily converted from
one form to another, depending on conditions, according to the dispropor-
tionation reaction, 2Hg(I) J Hg(0) + Hg(II)  (Hem, 1970; Jonasson and Boyle,
1971).  The stability and solubility of each of these oxidation states is
dependent upon the redox potential, the electrical potential  (EL)-pH
relationship, temperature, and the concentration of major ions in solution.

      Four basic reactions are involved in the mercury interconversion
cycle (Figure 3.1): (1) conversion of Hg(0) to Hg(II), (2) conversion of
inorganic Hg(II) to methylmercury, (3) conversion of phenyl-  to methyl-
mercury, and (4) conversion of alkoxyalkyl to Hg(II) or Hg(0)  (Jernelov,
1969; Jonasson and Boyle, 1971)-  These reactions permit release of mercury
to enhance its movement in soil, water, sediment, and biota.

      Metallic mercury, Hg(0), is somewhat reactive: it can be oxidized or
reduced in the presence or absence, respectively, of oxygen (Hem, 1970).
Its water solubility,  0.02 to 0.03 mg/fc at 20 C is higher for the metal
than for some of its salts (Krenkel, 1974).  Metallic mercury has a marked
chalcophile character and, in natural systems, forms sulfides, selenides,
a telluride, and complex compounds with antimony, arsenic, and sulfur
(Jonasson and Boyle, 1971).  It is also an oxyphile forming a natural oxide
and a number of oxychlorides and complex hydrous chloride sulfates.
                                   3-1

-------
                 Bacterial oxidation
                 Plankton
                 Plants
                 Inorganic
                 reactions
                                          Mercuric ion,
                                          chelated cations and onions,
                                          simple complexes,
                                          oxides,  sulphides
                                               Hg(ll)
                           Bacterial reduction
                        Fungi
                     Plant:
                   Inorganic reactions
                 Sunlight
Elemental mercury
as vapour, liquid
or dissolute
        Hg(0)
Bacteria
Sunlight
                ^Bacterial reduction
                  ^Fungi
                    ^Plants
Bacterial oxidatiorrv  \Jnorganic
Plants             >«.   ^v^reactions
Inorganic reactions
                                                           Bacterial synthesis
                                                           Chelation
                                                        Bacteria,    ^'
                                                        conversion by
                                                        organic oxidants
	  fr
                %
                                                          £•  R-Hg-R'
                                               Hg(l)
                                                                       Bacterial synthesis
                                                                       Chelation
                                                                       Organic oxidants
 Duproporlionation and
   electron exchange
                                 Mercurous ion,
                                 chelated cations and onions,
                                 simple complexes
Organo - mercury
   compounds

R, R' = alkyl, aryl,
      mercapto,
      protein, etc.

X = monovalent anion
   eg. halide, acetate,
   etc.
                  Figure  3.1.   Cycle  of  mercury  interconversions  in
                                    nature (Jonasson  and  Boyle,  1971).
                                               3-2

-------
ultraviolet radiation lose  the ability  to methylate mercury  and  that  the
rate of conversion to methylmercury  is  dependent upon mercuric ion  concen-
tration, solution pH, and temperature.

      Pathways which have been proposed for methylation  of mercury  (Jernelov,
1969; Wood, 1971; Katz,  1972; Bisogni and Lawrence, 1973; Rogers, 1975, 1976)
are summarized in Figure 3.2.  Several  kinetic  schemes have  been proposed by
Wood  (1971) ,  indicating  that most  anaerobes and many aerobic bacteria can
synthesize methylmercury.   Under anaerobic conditions, both  organic and
inorganic mercury compounds are first converted to metallic  mercury,  then
transformed to methylmercury; under  aerobic conditions,  metallic mercury
and organic mercury compounds are  converted to  mercuric  ions before methyl-
ation occurs.  The formation of methylmercury is predominant at  lower pH,
while dimethylmercury is favored at  higher pH.  Monomethylmercury is  mainly
accumulated in living organisms, while  dimethylmercury has a tendency to
evaporate to  the atmosphere.  The  released dimethylmercury may break  down
under ultraviolet light  or  acidic  conditions to monomethyl-  or elemental
mercury and will precipitate over  land  or water.  Monomethylmercury may
convert to dimethylmercury  or to volatilizable  mercuric  ions (Jernelov,
1972).

      The rate of biological methylation of mercury appears  to be largely
dependent upon the concentration of  mercury, the number  of methylating
organisms, and physical-chemical parameters including pH, temperature,
and the presence of oxygen  and other organic materials.  Fagerstrb'm and
Jernelov (1972) found in laboratory  experiments that the rate of methyl-
mercury formation in organic sediments  is much  higher when the oxygen
content in water if low, 0  to 2 ppm.  The subsequent uptake  by fish is
also higher at low oxygen levels.  In natural waters, a  higher rate of
methylation can occur under aerobic  rather than anaerobic conditions  due
to the formation of mercuric sulfide (Jernelb'v, 1972) .   Methylation of
mercuric sulfide is not  likely to  occur under anaerobic  conditions; even
under aerobic conditions, it is 100  to  1000 times slower than with mercuric
chloride (JernelBv and Martin, 1975).   The rate of mercury methylation under
anaerobic conditions seems  to be slow and of less significance ecologically
than that under aerobic  conditions (Katz, 1972).  When sediments are  exposed
to air by the action of  tides or by  dredging activities, the rate of  methy-
lation is higher by orders  of magnitude than the normal  rate for sediments
under water (Fagerstrb'm  and Jernelov, 1972) .  Bisogni and Lawrence  (1972)
have found the rate of mercury methylation to be much higher in  aerobic
systems than in anaerobic systems  at a  given mercury concentration and
microbial growth rate.

      Jacobs and Keeney  (1974) found a  much higher degree of methylation in
the sediments of the Wisconsin River with low pH and high organic matter
than in the sediments of the Fox River  which is characterized by high pH and
low organic matter.  Temperature also influences the rate  (Fagerstrtim and
JernelSv, 1972).  The methylation  rate  increases with temperature, roughly
following the QiQ-rule (doubled rate when temperature increases  10 C), and
with increased nutrient  content in the  substrate.  The rate  is also higher
with suspended material  and in the upper layer  of sediment than  it  is
farther down.

                                     3-4

-------
      Compounds of Hg(I) are typical  of metallic  mercury compounds (Krenkel,
1974):  (1) all are ionized  in solution;  (2) most,  except for the nitrate and
perchlorate, are only sparingly  soluble in water  at  ordinary temperatures;
and  (3) mercurous chloride  (HgCl),  the most soluble  of  the halides,  occurs
naturally.  They are readily oxidized to Hg(II) by strong oxidizing  agents.
Salts of Hg(I) undergo disproportionation to  give  Hg(II)  derivatives and
Hg(0).  The mercurous ion,  Hg(I), does not form covalent bonds  with  other
elements, and thus organometallic Hg(I) derivatives  do  not occur.

      The mercuric ion, Hg(II),  is  relatively stable under oxidizing condi-
tions,  especially at low pH (Hem, 1970), but  can be  reduced to  Hg(I)  or
Hg(0) in a reducing environment  (Jonasson and Boyle, 1971;  Holm and  Cox,
1974).  When organic materials are  present, divalent mercury will  also bind
to them to form organic complexes.

      Mercuric mercury, Hg(II),  generally forms covalent  rather than ionic
bonds,  although the nitrate Hg(NC>3)2, sulfate HgSO^, and  perchlorate
Hg(C104)2 are true salts (Krenkel,  1974).  These compounds  are  less  soluble
in water than in organic solvents.  The Hg(II) cation is  not a  common
species in aqueous media and never  constitutes a significant portion of  the
total mercury found there.

      Carbon bonds more stable with Hg(II) than with Hg(I)  (Krenkel,  1974).
Both mono- and diorganomercury (II) derivatives are  well  known.  Alkylmer-
curic compounds are more stable  and less volatile but are more  mobile
biologically than other forms of mercury (D'ltri, 1972).

      The biological conversion  of  inorganic  mercury to organic methyl-
mercury is the key to the distribution, transport, and bioaccumulation of
mercury in the environment  (Jensen  and Jernelb'v, 1969; D'ltri,  1972).  In
aquatic systems, this conversion occurs by two main  processes,  microbial
(enzymatic) and chemical (nonenzymatic).  Enzymatic  methylation  takes place
in organic sediments and in rotting fish (Jensen and Jernelov,  1969), in
cell extracts of methanogenic bacterium (Wood et al., 1968),  in  cultivated
bacterium Clostridium (Yamada and Tonomura, 1972), and in  fungus Neurospora
(Landner, 1971).  Nonenzymatic synthesis of methylmercury  from  inorganic
divalent mercury occurs under mild  reducing conditions with  methylcobalamin
and propylocobalamin (Wood  et al.,  1968).  Methylmercury  is  also formed  from
mercuric chloride by a purely abiotic reaction in the presence  of  methyl-
cobalamin (Imura et al., 1971; Bertilsson and Neujahr, 1971).   However,
microbial activity is a prerequisite  for methylation in nature  unless other
methylmetal compounds are already present in  the system  (Jernelov  and Martin,
1975).  Laboratory experiments have shown that mercury methylation also
occurs  in the terrestrial environment in soils (Berkert et  al., 1974; Rogers,
1975) and in the tissues of peas  (Gay, 1976) .

      Rogers (1975, 1976) at the EPA  laboratory in Las Vegas  has found that
the soil transformation of  mercury  to methylmercury  occurs by abiotic as
well as biotic means.  He determined  that a constituent could be extracted
from soil which would methylate mercuric ion  abioticly.  The methylating
substance is associated with the lower molecular weight  fraction of  the
soil organic matter.  He also found that soil extracts exposed  to

                                    3-3

-------
                                                            ATMOSPHERE
U>
(C6H5)2 Hg
Diphenyl Mercury



Phenyl
Mercuric
Ion
            CH30-CH2-CH2-Hg

          Methoxyethyl Mercury
                                               Hg
                                                  Hg
       Hg°
 Metallic Mercury

        -»	
                                                              enzyme  transfer
                                                                (anaerobic)
                    (CH3)2 Hg

                Dimethyl Mercury
                                          Mercurous Ion
   Mercuric Ion
                 	chemical  transfer
(aerobic)
                                                             (also abiotic in soil)
    CH_ Hg+
      j
Methyl Mercury
                                                HgS

                                          Mercuric Sulfide
                          Figure 3.2.
       WATER  (SOIL)

Pathways of mercurial breakdown and methylation  in  nature
(Jernelov,  1969; Wood, 1971;  Katz,  1972;  Bisogni  and
Lawrence, 1973; Rogers, 1975  and  1976).
                                                                                                             AQUATIC ANIMALS

-------
      Matsumura et al. (1971) found that microorganisms isolated  from
mercury-containing soil and lake sediments convert phenylmercuric acetate
to diphenylmercury but observed no methylmercury derivatives after 10-day
incubations.  During long-term, 50 days, incubation of lake sediments
containing inorganic mercury, Spangler et al.  (1973) observed a buildup
of low concentrations of methylmercury, up to  0.3 yg/g sediment.   There was
a rapid decrease in the amount of methylmercury due to microbial  degrada-
tion followed by the evolution of elemental mercury, suggesting that  the
net amount of methylmercury accumulation in the sediment would be minimal.
Degradation of methylmercury has been observed in cultures of bacteria
Pseudomonas (Tonomura et al., 1968), in rats (Norseth and Clarkson, 1970),
and in the guinea pig (Iverson and Hierlihy, 1974) .
GEOCHEMICAL CYCLE OF MERCURY

      The processes by which mercury is transportated through the geochemical
cycle are shown in Figure 3.3 (Jonasson and Boyle, 1971).  Because of its
high vapor pressure, even at normal temperatures, elemental mercury rapidly
vaporizes from rocks, soils, and waters into the atmosphere.  At room
temperature, the saturation concentration of mercury in air approaches 15
mg/m3 (Vostal, 1972).  Mercury is also released to the atmosphere by
volatilization, chemical or microbial reduction, and biological methylation.

      Solubility is a major factor in the natural distribution of mercury.
Practically all mercury in air disappears immediately after a rainstorm,
even in areas of higher concentration such as near ore deposits (McCarthy
et al., 1970).  Despite a tendency to sorb on soil, plants, or animals or
to complex with other organic materials and minerals, mercury compounds are
translocated within the soil or into the hydrosphere by surface runoff,
groundwater, or geothermal springs.  Weathering and sedimentation are the
major pathways for the natural flux of mercury between land and the oceans.

      The summation of available data on the global cycling of mercury from
natural and man-made sources indicate that the total release of mercury in
nature due to weathering and vaporization amounts to 20,000 to 40,000 metric
tons per year, while man's activitiy contributes an additional 5,000 to
10,000 metric tons per year (Table 3.1) (Hammond, 1971; Dickson, 1972;
Wollast et al., 1975).  While these data may be open to question,  they
suggest that man's activity could increase the natural flux by up to 60
percent.

      The residence times of mercury in the four available reservoirs
(Wollast et al., 1975) are reported as:
                         Reservoir       Time

                         Land         1,000 years
                         Atmosphere   60 days
                         Ocean        32,000 years
                         Sediments    2.5 x 108 years
                                    3-6

-------
1
DEGRADATION

1
I
1
! ATMOSPHERE
i
i

c
^Py^DE
/
_ EXHALATION
PRECIPITATION

BIOSPHERE
Plants ^»^ Animals
C/tGkAD/-T|( )N

1 + Vo
GRADATION ABSORPTION ^\^o
AND AND <3snVr'<
SOLUTION ADSORPTION ^ftoV'
i I % 1

HYDROSPHERE
Water ^^ Sediments

i t
CHEMICAL PRtCll'ITATIOrJ , (- . r,-)N,
AND SEDIMENTATION ',",,^'J;N
OF SOLIDS ' ' ' ,,
, WtAlHtfh
f I
EXHALATION
HEMICAL
PRECIPITATION
LiTHnSPHEPE i
PRECIPIIAilON |
*" FhDUbPhckL-
Sors
SOLUTION /-., • i ,. . ,

1
1
VND
Al
slG
OOlUTlON AND
Mr.CHA-'.lCAL
WEATh: y.M'S
ftoc^s !
Mercury Deposits ___ ;/f tGfVAiiON A^aJ
voKANK.pHtNov.FNA * TONSOl , ! ; A T ION OF SOI IDS
Figure 3.3.  Generalized geochemical cycle of mercury
             in natural systems (Jonasson and Boyle, 1971).
                         3-7

-------
                                     TABLE 3.1.  GLOBAL CYCLING OF MERCURY

                                             (metric tons/year)
I
oo
Man's Activity
Natural Flux
Weathering
230
3,800
2,500
4,000
5,000
Vaporization
NAa
>25,000
NA
36,000
25,000
Industrial Wastes
3,000
5,800
NA
NA
5,000
Fossil Fuel
Combustion
(Coal and Oil)
3,000b
1,600
1,600
3,000-5,000
5,000
Total Flux Reference
Joensuu, 1971
36,000 Weiss et al., 1971
Bertine and Goldberg,


1971
43,000-45,000 Marton and Marton, 1972
40,000 Wollast et al., 1975

      NA = Not available.



      Coal only.

-------
The tentative present-day  geochemical cycle of mercury in the biosphere is
shown in Figure  3.4.
                                              Total flux to atmosphere
                                                     408
1 1
LAND
gain 23
/
\
Rivers
50
\ f
^ OCEAN
gain 64
/
                            90
M                                   UPLIFT"
                                     32
EXPOSITION
   35
                            MINING
                                     SEDIMENTS
                                      lOSS 87
      Figure 3.4.  Tentative present-day  cycle  of  mercury (reservoir
                   changes and  fluxes  in  units  of  102  MT/yr).   [Reprinted
                   from Ecological  Toxicology Research (A.  D.  Mclntyre
                   and C. F. Mills,  editors) by R.  Wollast,  G.  Billen,
                   and F. T. Mackenzie by permission of Plenum Publishing
                   Corporation.  Year  of  publication 1975.]
Air Transport

      Mercury  is  readily  emitted  to  the atmosphere due largely to its
volatility and high vapor pressure.   Presumably,  in the U.S.,  over 500
metric tons of mercury  are  emitted to the atmosphere each year from man's
activity  (Holt et al.,  1975).   Mercury and its compounds are dispersed in
air by wind and are deposited  on  land and water by dry precipitation,
rainfall, and  snowfall.   However, the mode of  transport and fate of this
pollutant are  not clearly understood.

      The ambient forms of  mercury in air include particulates,  mercuric
chloride-type  compounds,  methylmercury (II-type compounds), elemental
mercury, and dimethylmercury  (Braman and Johnson, 1975).  Vapors tend to
concentrate by adsorption on dust particles (Williston, 1968)  and, together
with the other particulates, are  removed by dry fallout, rain, and snowfall
(Jenne, 1970) .  The saturation concentration of mercury in air amounts to
10 to 15 mg Hg/m3 at room temperature (Vostal, 1972).

      While the rate of mercury release into the  atmosphere is determined
mainly by barometric pressure,  its distribution and transport  are largely
controlled by  wind, rainfall,  and snowfall, seasonal variations  in
temperature, and  other natural and meteorological laws (McCarthy et al.,
                                    3-9

-------
1970).  Williston (1968) found higher, nearly double, mercury  concentrations
in summer than in winter in the San Francisco Bay area.  Diurnal  variations
have  also been observed (McCarthy et al., 1970).  Generally, the  greatest
amounts are found at midday, while minimum concentrations are  observed  in
the morning and evening when the rate of increase in barometric pressure is
high.  Higher mercury readings have been observed on cloudy and foggy days
than  on normal days, yet no clear correlation has been found between
humidity and mercury content of the air.  Elevated atmospheric concentra-
tions of mercury also have been observed on smoggy days  (Krenkel  and
Goldwater, 1973; Williston, 1968).  Cool, wet weather generally results in
lower than average mercury readings, whereas the reverse is true  for warm,
dry weather.  However, the daily maximum mercury reading does not neces-
sarily coincide with the daily maximum temperature (McCarthy et al., 1970).

      More mercury is found in the air of mercury mines and mineral
deposits than elsewhere (McCarthy et al., 1970).  However, the dependence
of mercury content on the distance from a source is more likely a function
of mercury species and the prevailing wind.  In studies around sewage plants
in Washington, D.C., and Memphis, Tennessee, the concentration of elemental
mercury in air decreased sharply with increasing distance from the source,
while the organic mercury forms increased almost logarithmically with
distance up to at least 16 km (10 mi) (Soldano et al., 1975).  This suggests
that  the alkylmercury compounds can be spread over a broad area and long
distance from the source, a phenomenon which is enhanced by the prevailing
wind  direction and velocity in the area (Anderson and Smith, 1977) .

      The vertical distribution of mercury in air appears also to be a
function of altitude (McCarthy et al., 1970).  Maximum levels above the soil
surface near mercury mine areas are reportedly nearly 200 times greater than
those found at 122 m (400 ft)  above the ground (Table 3.2).  This altitude
effect is much less significant in nonpolluted areas and varies according
to seasonal/temperature differences (Williston, 1968).  Williston (1968)
suggests that mercury is uniformly distributed in soil throughout the
United States by wind action as well as by rainfall and snowfall.
Soil Transport

      The mercury concentration of soil varies significantly at different
locations.  In addition to the naturally occurring mineral deposits, large
quantities may be deposited from the atmosphere and a lesser amount may be
added by agricultural applications such as pesticides and seed treatments.

      Factors influencing the mercury content of soils include pH, drainage
composition, concentrations of humus, and microbial activity.  Mercury
compounds may convert to soluble or volatile forms by geochemical and
biological actions within the soil.  Metallic mercury may be readily
volatilized or converted to mercuric sulfide or, due to its high affinity
for organic matter, to organic mercury compounds.  Thus, the accumulation
of mercury in soil may be related to the total sulfide and organic carbon
levels (Lagerwerff, 1972).
                                    3-10

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        TABLE 3.2  MAXIMUM MERCURY CONCENTRATION IN AIR MEASURED AT
                   SCATTERED MINERALIZED AND NONMINERALIZED AREAS
                   OF THE WESTERN UNITED STATES
                                               Maximum Concentration, ng/m3
                                                 Ground      122 m  (400 ft)
            Sample Location                      Surface     above  ground

                               Mercury Mines

Ord mine, Mazatzal Mtns., Arizona              20,000 (50)b     108   (4)
Silver Cloud mine, Battle Mtn., Nevada          2,000 (50)       24   (8)
Dome Rock Mtns., Arizona                          128  (6)       57  (20)

                        Base and Precious Metal Mines

Cerro Colorado Mtns., Arizona                   1,500  (5)       24   (2)
Cortez gold mine, Crescent Valley, Nevada         180 (60)       55   (4)
Coeur d'Alene mining district, Wallace, Idaho      68 (40)         NA
San Xavier, Arizona                                 NA           25   (3)

                           Porphyry Copper Mines

Silver Bell mine, Arizona                           NA           53   (3)
Esperanza mine, Arizona                             NA           32   (3)
VekolMtns., Arizona                                NA           32   (4)
Ajo mine, Arizona                                   NA           30   (3)
Mission mine, Arizona                               NA           24   (3)
Twin Buttes mine, Arizona                          20            22   (3)
Pima mine, Arizona                                  NA           13   (3)
Safford, Arizona                                    NA            7   (2)

                             Unmineralized Areas
Blythe, California                                  NA            9  (20)
Gila Bend, California                               NA            4   (2)
Salton Sea, California                              NA            3.5  (2)
Arivaca, Arizona                                    NA            3   (2)
Source: McCarthy et al., 1970.

aSamples taken from single-engine aircraft.

 Number of measurements shown in parenthesis.

CNA - Not available.
                                    3-11

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      A linear relationship exists between humic acid  and mercury  content  in
soil  (Trost and Bisque, 1972).  Humus-rich soil also increases  the solubility
of inorganic mercury compounds, such as mercuric sulfide, mercuric oxide,  and
mercurous chloride, above their normal levels.  Over 1,000 ng of mercury have
reportedly been absorbed on 1 g of organic matter in soil (Andersson,  1967;
Eriksson, 1967).  This reaction is enhanced at lower pH.

      Moisture affects the vaporization of mercury compounds at the soil
surface; vaporization decreases with increasing soil moisture (Williston,
1968; Kimura and Miller, 1964).

      Although they are more chemically stable, methylmercury compounds are
not bound as firmly to soil or other organic mercury compositions.   Aryl and
alkoxyalkyl mercury salts decompose more readily in soil and liberate  ionic
and/or metallic mercury (Rissanen and Miettinen, 1972) .  Up to 16  percent of
phenylmercury acetate and 32 percent of ethylmercury acetate may volatilize
as metallic mercury vapor in 28 days and 53 days, respectively  (Kimura and
Miller, 1964).  Methyl derivatives are volatilized as methylmercury at a
rate  of 6 to 14 percent in 35 days without decomposing to metallic  mercury
vapor.  From 51 to 53 percent of methylmercury dicyaniamide added  to soil
may be retained after 5 months (Saha et al., 1970).

      In microcosms, 139 days after the application of 2Q3Hg, Huckabee and
Blaylock (1974) reported that 50 percent of the total 203Hg remained in the
terrestrial system and 50 percent reached the aquatic system.  About 90 to
97 percent of the mercury in the terrestrial system was bound to the soil;
only  3 to 7 percent was retained on the litter and less than 1 percent
absorbed by plants.  Old field ecosystem studies have indicated that nearly
80 percent of 203Hg applied to soil is retained at the end of the  growing
season (4 months) (Matti et al., 1975).  The amount of mercury increases
with  time, and more than 90 percent is confined in the top 3 cm of  the
column.  Levels of mercury in plants bear little relationship to soil
levels since most plants grown in mercury-treated soils accumulate  such
small amounts (Smart, 1968; Blanton et al., 1975).
Water and Sediment Transport

      Much of the mercury entering surface waters is rapidly adsorbed or
bound to suspended materials and living organisms (Jenne, 1970).  Acidity
is important for the degree of binding.  Mercury solutes introduced into
streams are quickly transformed to particulates by reduction to metallic
mercury, sorption onto inorganic sorbates, complexation with nonviable
particulate organics, and sorption and ingestion by biota.  The majority
of all forms of mercury in water accumulate in the bottom sediment
(Vostal, 1972).  In groundwaters, the bicarbonate content is directly
related to mercury concentration (Jenne, 1970).

      The mobility of mercury in water is mainly determined by the presence
and amount of organic materials and suspended sediments and the occurrence
of redox conditions (Jenne, 1970).  Elevated levels of dissolved organic
                                    3-12

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materials may increase mercury mobility.  A high percentage of mercury
introduced into streams  is removed as  sediment and associated fine-grained
materials.  Reducing  conditions may  increase mercury mobility by causing
the dissolution of manganese  and  iron  oxides and by releasing sorbed
mercury to be available  for complexing with other organics; they may also
decrease mercury mobility by  reducing  a significant amount of mercury
compounds to metallic mercury which  will  then amalgamate with iron oxides
or fall to the bottom.   Mercury becomes relatively immobile in reducing
sediments and precipitates as mercuric sulfide (cinnabar).  However, at
high pH or under strongly reducing conditions, mercuric sulfide anions may
become very soluble.

      Chlorides may also affect the  release of mercury from bottom
sediments.  The use of CaCl2  and  NaCl  for deicing roads increases the
relative amount of mercury in water  in equilibrium with sediments by 2 to
5 orders of magnitude (Feick  et al., 1972).  The changes in pH associated
with the addition of  these salts  may increase mercury mobility.

      The transport and  deposition of  mercury in estuaries is largely
governed by interactions with natural  organic matter (Lindberg and Harris,
1974).  In the estuaries of the Gulf of Mexico, approximately 54 to 82
percent of the total  dissolved mercury is associated with fulvic acid-type
materials.  Decomposition of  estuarine vegetation results in detritus
enriched in mercury.  Thus, in the Gulf of Mexico, mercury in estuarine
sediments is not significantly redistributed by postdepositional processes.

      Windom (1976) reported  that only 14 percent, 0.5 x 103 kg/yr, of the
mercury transported by several southeastern U.S. rivers is in particulates,
while the majority, 2.7  x 103 kg/yr, is in soluble form.  However, more
than 95 percent of the total  mercury in the Walker Branch streams in
Tennessee is in suspended forms.  The  contribution to total transport of
mercury by suspended  sediments is approximately 45 percent, while about
55 percent is due to  dissolved mercury (Andren et al., 1975).

      Up to 62 percent of the mercury  present in surface sediments is bound
to sulfur-containing  organic  and  inorganic particles, clays, and minerals
(Stopford and Goldwater, 1975).   In  deeper sediments, more mercury, up to
75 percent, is bound  to  organic acids.  Only a small portion of this mercury
is released to water.  Simulated  dredging results in a slight increase in
the total dissolved mercury in overlying water because of the mixing of
sediment with water and  short-term release of the mercury associated with
particulate materials (Lindberg and  Harris, 1974).

      Sedimented mercury can  be released by biological methylation processes,
Methylated mercury is quickly absorbed by living organisms or bound to
inorganic suspended particulates, or it may be rapidly photodegraded to
inorganic mercury and escape  to air.   Therefore, methylation processes
facilitate mercury transport  not  only  by releasing mercury from sediments
to water and air but  also by  movement  within the food chain.  However,
they may not play a major role in the  postdepositional migration of mercury
in sediments (Andren  and Harris,  1973).  Stopford and Goldwater (1975) and
Windom et al. (1976)  found little or no methylmercury in the sediments.

                                  3-13

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Although Windom et al.  (1976) detected a significant  level  of  methylmercury
in the dominant primary consumers of the estuarine  ecosystem,  the  estimated
annual methylmercury conversion was only about 50 ng/g  of the  total  mercury
in the upper 5 cm of the sediment column.  This  indicates that methylation
is an inefficient mechanism for transferring mercury  out of salt marsh
sediments.  Methylation of mercury is entirely restricted to the surface  of
the sediment with no macrofauna present (Jernelov,  1970).   If  sediment-
dwelling organisms are present at high densities, the release  of mercury  by
methylation may occur in sediment at depths to 9 cm.

      A study of the mechanisms for mercury transformations affecting
mobility in Par Pond at the Savannah River in Georgia has shown that the
reduction of mercury to its elemental form within a sedimentary environ-
ment is accomplished by humic acids serving as an extracellular electron
transport mechanism (Schindler et al., 1975).  The mercury  not liberated
from sediments by this mechanism is retained by the organic macromolecules
and alkylated to methylmercury.  This mechanism, however, accounts for
only a small portion of the mercury in the system.  The electron transport
capacity of the organic ligands may be blocked by calcium,  clays, or other
cations and effectively suppress the alkylation/reduction potential of the
sediment.
FOOD CHAIN TRANSPORT

      Most aquatic organisms can accumulate mercury in their tissue.
Concentration factors of mercury in edible aquatic organisms are 3 to 5
orders of magnitude greater than background levels (Thompson et al., 1972)
(Table 3.3).  The concentration by plants and fish appears to be at the
same level of magnification, but uptake by aquatic invertebrates is much
greater, reaching up to 100,000 times the surrounding water level.  Since
invertebrates are not at the top of the food chain, it can be assumed
that the biological magnification of mercury could be a function of the
particular species in the trophic level.  However, the levels of mercury
in general increase with increasing trophic level (Table 3.4).  Mean
mercury levels in predators are nearly 15 times greater than those in
algae eaters.  Aquatic organisms accumulate mercury compounds directly
from ambient water and from their food.  They absorb both inorganic and
organic methylmercury compounds.

      Neither uptake routes nor the relative importance of mercurials in
food chain accumulation and transport have been fully quantified.  The fact
that methylmercury compounds are the predominant form of mercury in aquatic
organisms even though methylmercurials are never found in abundance in
water indicates that mercury in aquatic organisms is accumulated mainly
through the food chain (Huckabee and Goldstein, 1975).  A series of
laboratory experiments has shown that methylmercurials have a higher
assimilation efficiency and longer biological half-life than inorganic and
other organic forms of mercury.  Species differences in biological half-
lives of methylmercury exist even within various fish species living in
the same environment (Vostal, 1972).  The rate of mercury uptake through
the food chain is higher in the top predator fish (60 percent for pike)

                                   3-14

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  TABLE   3.3.  CONCENTRATIONS (IN PPM WET WEIGHT)  AND CONCENTRATION  FACTORS
               OF MERCURY IN WATER AND EDIBLE AQUATIC ORGANISMS
             Media
   Mercury
Concentration
Concentration
   Factor
       Sea water                     3.0 x 10
       Marine plants                 3.0 x 10
       Marine invertebrates          1.0 x 10
       Marine fish6                  5.0 x 10
       Freshwater                    1.0 x 10
       Freshwater plants             1.0 x 10
       Freshwater invertebrates      1.0 x 10
       Freshwater fish               1.0 x 10
         -5
         -2
         0
         —2
         i
         -4
         -1
         -1
 1.0 x 10'
 3.3 x 104
 1.7 x 10"
 1.0 x 10"
 1.0 x 10"
 1.0 x 10"
       Source:  Thompson et al., 1972.
        Concentrations in edible portions, e.g., muscle and soft parts;
          concentration factor determined by dividing the concentration
       ,   in the organixm by the concentration in water.
        Representative values for the continental shelf and estuaries,
          not for open ocean.
       .Exclusive of phytoplankton.
        Molluscs and crustaceans.
       /~\
        Edible portion only, not whole fish.
  TABLE 3.4.  BIOLOGICAL MAGNIFICATION OF MERCURY IN THE AQUATIC FOOD CHAIN


Organisms
Algae eaters

Zooplankton eaters
Omnivores

Detritus eaters

Predators

Number
of
Samples
39

9
9

12

25

Concentration, ppm

Range
0.01-01.8

0.01-0.07
0.04-1.16

0.13-0.59

0.01-5.82

Arithmetic
Mean
0.05

0.04
0.45

0.54

0.73


More Numerous Organisms
Zooplankton, snails,
mayfly nymphs
Insect larvae, minnows
Insect larvae and adults,
scuds
Worms, clams, insect
larvae
Insect larvae and adults,
frogs
Source:  Bligh, 1971,
                                     3-15

-------
and relatively lower for bottom feeders (less than 25 percent)  (Jernelb'v  and
Lann, 1971)-   Thus it appears that direct uptake of mercury from water  is
more important in lower trophic level organisms, while the food chain is
the main route of uptake in higher level organisms.  Direct accumulation
of mercury compounds from water is similar for both feeder types.

      Information on the mercury content of aquatic organisms is extensive,
but the vast  majority of the data are presented as total mercury instead
of as the methylmercury form.  Information needed to clarify the actual
food chain transport mechanism for mercury is therefore greatly impaired.

      Bioaccumulation through the terrestrial food chain appears to be less
significant to man than the aquatic food chain since the main route is
dressed seed  -> game birds -> man (Rissanen  and Miettinen, 1972).   Other
terrestrial routes are much less obvious and require further study.
                                   3-16

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                        4.   OCCURRENCE  OF MERCURY  IN FOOD
MERCURY LEVELS  IN  FISH

      The major dietary  source  of mercury  for many humans is fish and shell-
fish.  Birke et al.  (1972)  concluded  from  a  study of human exposure to
methylmercury through fish  consumption  that  this source contributes at least
half of the mercury  intake  among fish eaters.  Mercury concentrations in
blood cells were approximately  twice  the concentration found in the blood of
persons who did not  eat  fish, further confirming the importance of fish
consumption as  a critical determinant of mercury intake.  Per capita fish
consumption in  the United States averages  only 18 g per day; comparable
values for Swedes  and Japanese  are  56 and  88 g per day, respectively
(Hugunin and Bradley, 1975).

      Following the  Canadian Food and Drug Administration's closure of
Lake St. Clair  (an international boundary  lake) to commercial fishing in
March, 1970, and subsequent discovery that game fish in Lake St. Clair,
Michigan, had up to  7.0  ppm mercury and in Lake Champlain, Vermont, up to
2.5 ppm, the U.S.  Food and  Drug Administration Compliance Program was
initiated (Schroeder, 1974).  Serious mercury contamination was found in
25 rivers and bays in 11 states.  Of  763 composite samples of fish, nearly
90 percent showed  mercury levels below  0.5 ppm.  The highest mercury levels
were consistently  observed  in tuna.

      To determine the extent of the  problem, the entire canned tuna supply
of the United States was analyzed (Kolbye, 1972).  Results disclosed that
less than 4 percent  of the  tuna examined exceeded FDA's administrative
guideline for 0.5  ppm mercury.  A similar  sampling program for swordfish
revealed that less than  5 percent of  the swordfish samples were within the
0.5 ppm guideline  and 53 percent exceeded  1  ppm (Clark, 1973).  In May,
1971, a public  warning was  issued against  the consumption of swordfish.
Since 1971, all swordfish offered for sale in the United States are examined
for mercury and sold only after individual fish certification (Kolbye, 1972).

      In 1971,  FDA also  conducted an  extensive analysis program of the 19
most commercially  important fish.   The  mean  mercury level in saltwater fish
was approximately  0.09 ppm; however,  mercury concentration in a number of
fish of certain species, such as snapper,  bonito, and mackerel, were above
the 0.5 ppm level.   Seizure or  recall actions were initiated against 14
lots of snapper and  3 lots each of  bonito  and mackerel out of over 1,000
lots examined.  Other predatory fish  have  been implicated also but not in
commercially significant quantities (Kolbye, 1972).
                                    4-1

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      Swedish studies have indicated that fish caught  in inland waterways
and coastal waters often have much higher levels of mercury than  deep  sea
fish, suggesting the etiologic role of mercury-containing  industrial
effluents in causing excessive mercury uptake in fish  from these waters
(Hugunin and Bradley, 1975).  Although average levels  of mercury  in fish
seem difficult to assess, the most significant sources of  variation relate
to species, diet, age, size, metabolic rate, and habitat (Hugunin and
Bradley, 1975).  Table 4.1 represents a condensation of data presented by
Peyton et al. (1975) grouping fish according to nominal mercury content.

      Examining the mercury content of commercial canned shrimp, oysters,
clams, salmon, tuna, anchovies, cat food, and others,  Hall (1974) obtained
values for "percent methylmercury" that are in .general agreement with  those
of several similar studies reported in the literature  reviews by Hugunin
and Bradley (1975) and D'ltri (1972) (Table 4.2).

      In a study of fish from waters of the southeastern United States
which were suspected of being polluted by the chlor-alkali industry,
Granade (1972) concluded that a serious mercury problem exists in this
area and that mercury poisoning could result from a consistent diet of
fish caught from certain parts of the rivers.  The mercury levels reported
by Granade (1972) include the highest values reported  in Table 4.3.
Follow-up studies are not available to evaluate the degree to which
pollution continues in these systems.

      Henderson and Shanks (1973), reporting the results of a nationwide
monitoring program conducted by the U.S. Bureau of Sport Fisheries as part
of the National Pesticides Monitoring Program, found the highest residues
were reported in fish from the Columbia, Snake, Salmon, Rouge, and
Truckee rivers; the lowest levels were in fish from Alaskan streams and
the Colorado River (Table 4.3).  Eleven samples exceeded the FDA action
level of 0.05 ppm; however, this is significant only in those fish
destined for human consumption.  Not all the species sampled in this
program would normally be eaten (Henderson and Shanks,  1973).  Van Meter
(1974) , focusing on the evaluation of concentrations of mercury in muscle
tissue of game fish, studied the "effective" measure of exposure in terms
of human dietary dosage.  He reported that tissue concentrations of mercury
were low in fish from most of the Upper Clark Fork River region of Montana,
an area noted for diverse mining operations.  Consistently higher mercury
levels were found in fish taken from Flint Creek which is  fed by several
tributaries from a mining district (Table 4.3).
MERCURY LEVELS IN FOODS EXCLUDING FISH

      Although the high levels of mercury in fish and seafoods indicate that
these sources potentially contribute a majority of mercury intake, examina-
tion of mercury levels in nonfish commodities is important because:

      (1)  Fish and shellfish represent a relatively insignificant
           portion of the average American diet.


                                     4-2

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                 TABLE 4.1.   SUMMARIZATION OF FISH TYPE BY TOTAL MERCURY CONTENT (IN PPM)
0-0.10
Bloater
Crayfish
Chub
Hatchery trout
L. whitefish
Salmon
Shiner
Crab
Clam
King crab
Lobster tail
Oyster
Scallops
Shrimp
Other shellfish
Alewife
Anchovies
Butterfish
Cod
Fish sticks
Haddock
Herring
J. mackerel
Ling
Ocean perch
Salmon
Sardines
Seafood plate
Smelt
TV dinners
Whitefish
Whiting
Flounder
0.11-0.25
Bluegill
Buffalo
Bullhead
Carp
Catfish
Crappie
Coho salmon
Goldeye
Goldfish
Gar
Pumpkinseed
River carp
Rockbars
Shad
S. mullett
Sturgeon
Sunfish
Trout
D. crab
Lobster
Dolphin
Halibut
Pollock
Rockf ish
Scup porgy








0.26-0.45 0.46-0.65 0.66-0.99 1.00-1.70 5.5
Drum Eel Muskellunge Crevelle jack Blue marlin
N. pike Bluefish N. squawfish Grouper
Perch Dogfish shark Pickerel Ocean catfish
Sanger Gag Walleye Swordfish
Sheephead Lingcod Redfish
Sucker Mullett S . mackerel
Whitebass Sea catfish
Bonita Sea trout
Croaker White marlin
K. mackerel
Menhaden
Pompano
Red snapper
Sablefish
Tuna
Bass

















Source:  Peyton et al., 1975.

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               TABLE 4.2.  MERCURY RESIDUES IN CANNED SEA FOOD
Methylmercury ,
Sample Product ppmd
1
2
3
4
5
6
7a
8a
9
10
11
12
13
14
15
16b
17
18
19
20
21
22
23C
24C
25
26
27
28
29
30
31
32
33
34
35
36
Shrimp
Oysters
Oysters
Minced clams

Clam sauce
Cat food

Sardines
Anchovies
Kippered herring
Pink salmon


Red sockeye salmon
Coho red salmon
White tuna





Tuna

Light tuna











0.007
0.020
0.027
0.029
0.088
0.012
0.019
0.0.15
0.021
0.090
0.081
0.009
0.023
0.015
0.045
0.025
0.31
0.20
0.25
0.32
0.22
0.34
0.28
0.35
0.68
0.50
0.31
0.25
0.53
0.33
0.23
0.40
0.15
0.29
0.18
0.29
Total Mercury,
0.009
0.037
0.033
0.046
0.047
0.033
0.035
0.039
0.038
0.21
0.13
0.016
0.064
0.020
0.047
0.018
0.38
0.24
0.39
0.38
0.20
0.43
0.36
0.39
0.73
0.57
0.45
0.21
0.60
0.43
0.24
0.55
0.13
0.26
0.20
0.33
Methylmercury ,
78
54
82
63
187
36
54
38
55
43
62
56
36
75
96
139
82
83
64
84
110
79
78
90
93
88
69
119
88
77
96
73
115
112
90
88
Source:  Hall, 1974.
 Samples 7 and 8 had the same lot number.
 Average percent methylmercury in tuna Samples 17-36 = 89 percent.
.Samples 23 and 24 had the same lot number.  Both cans were unlabeled.
 Determined by gas-liquid chromatography.
Determined by atomic absorption spectrometry.
                                    4-4

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                            TABLE 4.3.  MERCURY CONTENT OF SELECTED FOOD FISH
J>
I
Concentration, ppm
Location
Region 1
Pacific and Alaska

Region 2 Southwest
Date

1969
1970
1969
Region 3 North Central 1969
Region 4 Southeast
Region 5 Northeast
Alabama
Florida
Georgia
Kentucky
Murray area
Montana
Ninemile Creek

Clark Fork River


Flint Creek
Rattlesnake Creek
Ohio, Sandusky
Michigan , Saugatuck
Wisconsin, Bay field
1969
1969
1970
1970
1970
NA


NA
NA
NA
NA
NA
NA
NA
1968-
1969

Organism

Fish
Fish
Fish
Fish
Fish
Fish
Fish
Crabmeat
Fish
Unspecified,
cooked

Trout
Whitefish
Shiner
Sucker
Squawf ish
Brown trout
Sculp in
Unspecified


Sample Site

Composite
Composite
Composite
Composite
Composite
Composite
Composite

Composite
Muscle


Muscle
Muscle
Muscle
Muscle
Muscle
Muscle
Muscle
Whole fish
and fish
livers
Mean

0.25
0.26
0.08
0.20
0.23
0.23
0.36
0.39
0.40
0.375


0.15
0.17
0.13
0.27
0.25
0.53
0.27
N.A.


Range Reference

0.06-1.25 Henderson and Shanks, 1973
0.05-1.7
<0. 05-0. 14
<0. 05-0. 50
<0. 05-1.0
<0. 05-0. 80
0.01-2.26 Granade, 1972
0.33-0.50
0.0-1.70
Dassani et al., 1975


NA Van Meter, 1974
NA
NA
NA
NA
ND
NA
<0.010 Lucas et al., 1970


   NA = Not available.

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       (2)  Concern exists  over  the  possibility that  the widespread
           usage of organomercurial preparations  in  agriculture
           in past decades may  have increased  the mercury content
           of crops.

       (3)  Data on nonfish commodities  are  essential in determining
           the relative  contribution of the various  food groups to
           total dietary intake of  mercury.

       The most important determinants of mercury  concentration  in food  are
 the environmental level  of mercury  in the area where a  food  is  produced
 and the use of mercury-containing compounds in agricultural  and industrial
 production of the food.  Organomercurials have been  widely used as seed
 preservatives and antifungicidal agents  sprayed on the  foliage  of many
 commercially grown fruits  and vegetables, especially fruit trees,  potatoes,
 and tomato plants (Dassani et al.,  1975; Hugunin  and Bradley, 1975).  Foliar
 application of phenylmercurials produces residue  levels  in crops  four to
 six times greater than those in untreated controls (D'ltri,  1972).

       Recently, usage of mercury in  seed dressings and  fungicides  has been
 severely curtailed.  The use of mercurial pesticides as  seed treatments
 was banned in February,  1976, and later reinstated in August, 1976.
 Production of these compounds must  cease by August 31,  1978, or earlier
 if the maximum allowed inventory (an estimated 50,000 pounds) is  reached.
 Any pesticides produced  before  the August deadline will  become  "existing
 stocks" which can be sold, distributed, and used  until  those stocks are
 depleted (Train, 1976; U.S. Environmental Protection Agency, 1976b).  Data
 presented by Dassani et  al. (1975)  and Gerdes  et  al.  (1974)  suggest that
 the application of mercury fungicides in the treatment  of wheat,  tomato
 plants, and fruit trees  is still in practice.

       In a "market basket" survey of common fresh  fruits and vegetables
 available in American supermarkets, Gerdes et  al.  (1974) assessed  the
 impact of the use of mercury in agriculture.  As  shown  in Table  4.4, 19 of
 23 common vegetables had mercury concentrations below 0.05 ppm.   Okra,
 cabbage, hot pepper, and string beans had mean mercury  concentrations
 greater than 0.05 ppm.  Higher levels were found  in  those fruits which had
 been sprayed with mercurial fungicides.  The observed differences  between
 commercially obtained peaches (0.063 ppm) and home-grown ones (0.019 ppm)
 (Table 4.4) may reflect  either application of  a mercurial fungicide on the
 commercial peaches or differences in the amount of mercury in the  environ-
 ment in the areas where  the peaches were grown.

      As shown in Table 4.5, levels in most foods other  than fish were
 below  the recommended maximum of 0.05 ppm (Joint  FAO/WHO Expert  Committee
 on Food Additives, 1972).  Staple foods grown  in  or  available in Michigan
were examined by Gomez and Markakis  (1974) for total mercury levels.
 Dairy and poultry products, red meats, cereals, fruits,  vegetables, and
miscellaneous items, along with many varieties of  fish  and shellfish,
were analyzed (Table 4.5).   Mercury concentrations in land-produced
 foods  (not suspected of man-induced contamination) were  in the  range 0.01
 to 0.03 ppm.   Fish samples, by comparison, contained larger  quantities of

                                   4-6

-------
TABLE 4.4.  MERCURY CONTENT OF FRESH FRUITS AND VEGETABLES
            COMMERCIALLY  AVAILABLE IN DENTON, TEXAS
Food Product
Fruits
Apple, red
Apple, yellow
Banana
Cherry
Grape , red
Grape, green
Grapefruit
Lemon
Lime
Melon
Nectarine
Orange
Peach
Peach, homegrown
Pear
Plum
Strawberry
Vegetables
Bell pepper
Black-eyed peas
Broccoli
Cabbage
Carrott
Cauliflower
Celery
Corn
Cucumber
Egg plant
Green onion
Hot pepper
Lettuce
Lima beans
Lima beans, dried
Mushrooms
Okra
Onion, white
Pinto beans, dried
Potato
Radish
Squash
String beans
Sweet potato
Tomato
Corn meal
Flour, white, bleached
Rice
Sugar
No. of
Samples

2
1
4
2
1
1
1
2
2
1
2
1
4
8
2
7
5

2
2
1
2
3
2
2
2
2
1
3
4
1
2
1
4
8
2
1
4
4
3
7
1
4
1
2
1
1
Concentration, ppb
Mean

14
86
85
8
113
88
31
110
111
8
97
92
63
19
45
124
48

37
5
26
59
5
24
16
16
10
47
15
55
20
46
19
43
73
44
17
34
4
3
51
21
31
98
103
87
51
Range

7-25
83-92
32-147
4-14
105-120
83-92
28-34
75-158
87-135
6-13
94-100
74-102
57-73
16-20
0-92
47-282
43-53

1-103
2-10
24-27
27-123
4-6
20-46
7-23
6-33
2-19
45-48
12-18
50-60
19-21
40-50
17-21
32-53
57-97
33-49
11-22
26-42
1-7
1-5
46-57
19-24
20-36
93-103
92-118
80-92
49-52
      Source:  Gerdes et al.,  1974.

                          4-7

-------
                           TABLE  4.5.  MERCURY CONTENT OF FOODS  (EXCLUDING FISH)
     Location'
     Food Product
No. of      Concentration, ppm
Samples   Mean        Range
    Reference
     Kentucky,
      western
00
     Michigan
Flour                            28
Grains, composite
  Oats
  Rice
  Wheat
Meats
  Bacon
  Chicken, gizzard
  Chicken, liver
  Ham, cooked
  Hamburger
  Pork, sausage
  Weiner
Vegetables and fruits
  Beans, green
  Beets, seed
  Carrot
  Carrot seed
  Onion
  Onion
  Potato
  Tomato, fruit
  Tomato, seed
  Fresh fruits composite

Bread, white                      2
Bread, white
Dairy
  Cheese, Cheddar                 2
  Cheese
  Milk, whole unpasteurized       2
          0.003b   0.003-0.06
                   0.002-0.048
          0.012
                   0.05-1.0
          7.15

          0.072
          0.007
          0.0009
          0.010
          0.011
          0.047
          0.008
                                                              0.017
                                                              0.042

                                                              0.030
                                                              0.007

                                                              0.012
                                                              0.05
                                                              9.45
                   0.00-0.28
                   0.00-1.09
                                                                       0.004-0.035
          0.01     0.005-0.01
          0.01

          0.02     0.015-0.03
          0.08
          0.01     0.01-0.02
Tanner et al., 1972
Goldwater, 1971
Dassani et al., 1975
D'ltri, 1972
Dassani et al., 1975
Hammons et al., 1975
Dassani et al., 1975

Hammons et al., 1975
Dassani et al., 1975
Goldwater,  1971

Gomez  & Markakis,  1974
Hammons et  al.,  1975

Gomez  & Markakis,  1974
Goldwater,  1971
Gomez  & Markakis,  1974

-------
TABLE 4.5  (Continued)
Q
Location
Michigan























Food Product
Dairy
Milk, whole pasteurized
Milk, spray dried
Eggs, whole
Eggs
Grains
Oats, garry grain
Rice, polished grain
Wheat, dickson grain
Meats
Beef, unspecified
Beef, liver
Beef, muscle
Chicken
Chicken, liver
Chicken, muscle
Pork, liver
Pork, muscle
Salt, noniodized
Sugar
Sugar, white
Vegetables and fruits
Beans, red kidney, dry
Potatoes, russet, flesh
No. of
Samples

2
2
3
33

1
2
2

23
2
2
24
2
2
3
3
2
22
3

2
2
Potatoes, russet, unwashed 2



North Dakota


peel
Potatoes, russet, washed
Tomato , fruit
Dairy
Milk, whole
Egg, melange

peel 2
2

155
405
Concentration, ppm
Mean

0.01
0.02
0.03
<0.002b

0.01
0.01
0.02

0.003
0.01
0.01
0.003
0.03
0.02
0.03
0.005
0.06
<0.003
0.01

0.03
0.01
0.03

0.01


0.0008
0.012
Range

0.005-0.01
0.01-0.03
0.02-0.04
<0. 002-0. 005

0.005-0.01
0.005-0.01
0.01-0.025

0.002-0.007
0.01-0.015
0.00-0.02
0.001-0.007
0.015-0.03
0.015-0.03
0.02-0.04
0.00-0.005
0.03-0.09
<0. 003-0. 010
0.005-0.01

0.02-0.035
0.01-0.015
0.02-0.03

0.01-0.02
0.00-0.01

ND-004
ND-0.442
Reference




Tanner et al. , 1972

Gomzz & Markakis, 1974



Tanner et al., 1972
Gomez & Markakis, 1974

Tanner et al. , 1972
Gomez & Markakis, 1974




Tanner et al., 1972
Gomez & Markakis, 1974








Sell et al., 1975


-------
                                         TABLE 4.5  (Continued)
a
Location
North Dakota



United States,
Location
unspecified





No. of
Food Product Samples
Egg yolk
Meats
Pork, liver 156
Beef, muscle 453
Milk, whole
Milk, whole 32
Milk, dry 33
Vegetable composite
Vegetables, fresh composite
Vegetables, canned composite
Potatoes

Concentration, ppm
Mean
0.062

0.015
0.005
NDC
<0.001b
0.010b




0.003b
Range

0.001-0.15
ND-0.304
ND-0.046

<0. 001-0. 009
0.004-0.027
0.00-0.06
0.002-0.010
0.005-0.025
0.006-0.020
0.001-0.015
Reference
Goldwater, 1971

Sell et al., 1975

Murthy, 1974
Tanner et al. , 1972

Goldwater, 1971
D'ltri, 1972
Goldwater, 1971
D'ltri, 1972.
Tanner et al. , 1972
 Indicates food grown in or available in the state mentioned.




 Median rather than mean given.




"Not detected or below detection limits.

-------
mercury; however, all samples were below the FDA action level for fish
(0.5 ppm).

      Concentrations of mercury  in animal products and soils of North
Dakota were reported by Sell et  al.  (1975).  Average levels in both muscle
and liver of beef and swine were considerably below 0.05 ppm (Table 4.5).
In fact, only 8 out of 399 beef  samples (2 percent) had mercury levels in
excess of 0.020 in muscles; only 3 samples of beef liver contained greater
than 0.05 ppm.  Marked variations were seen among individual eggs produced
at the same time and on the same farm, suggesting the possibility that
extraneous (nonfeed) mercury was being consumed by free-ranging hens.
Similar observations of wide variation in individual beef and swine also
suggest that some individuals may have incidental mercury exposures (Sell
et al., 1975).

      No apparent trends of increasing or decreasing exposure emerge when
data from recent studies are compared with those obtained by Stock and
Cucuel in 1934 and Gibbs in 1941 (Sell et al., 1975).  Mercury levels in
key foods of animal origin have  apparently remained about the same over
the past 30 to 40 years, according to Sell et al. (1975).  The analytical
techniques used in the earlier studies were undoubtedly less accurate than
today's methods and such comparisons should not be accepted uncritically.
MERCURY IN THE MARKET BASKET
AND TOTAL DIET

      The results of FDA's Total Diet Study, better known as the Market
Basket Survey, represents the most comprehensive measurements on the in-situ
levels of mercury in foodstuffs  (Peyton et al., 1975).  Not only do the
FDA's Total Diet Studies gather a representative cross-section from 30
markets in 27 different cities but also great  care was taken in these
studies to prevent sample contamination and to assure a sensitive and
accurate analysis (Peyton et al., 1975).

      A study by Tanner and Forbes (1975) involved more extensive sampling
of food, utilizing the FDA Total Diet Study protocol.  This "market basket"
approach is common to all of the studies discussed in this section and can
be described as follows:

      (1)  One hundred seventeen food items selected to represent
           the 2-week diet of an American male 15-20 years old are
           collected in various geographical locations at regular
           intervals throughout the year.

      (2)  Six composites of each of 12 food categories are
           obtained from 5 regions of the United States.

      (3)  Foods are weighed, prepared as they are normally eaten,
           and composited.  The composites are slurried and then
           analyzed.

                                   4-11

-------
All  commodity groups, except meat,  fish,  and  poultry,  contained less than
0.01 ppm mercury  (Tanner  and Forbes,  1975).   Mercury levels as high as
0.041 ppm were  found in the meat,  fish, and poultry group;  but when the
composite was analyzed component by component,  only the  fish component
had  mercury levels greater than 0.1 ppm.   The mercury  concentrations for
the  11 remaining  food groups of the Total Diet  Study were well below the
0.05 guideline  figure for foods other  than fish; mercury levels detected
in these foods  were generally less  than 0.01  ppm.   Only  the meat,  fish,
and  poultry food  group regularly contained measureable amounts of  mercury.

      Mercury ranked 20th in a list of 33 different  residues detected  by
the  FDA's Total Diet Survey during  FY 1971 in terms  of number of positive
occurrences (Manske and Corneliussen, 1974).  Mercury  residues were found
in 10 of 240 composites.  All 10 positive composites occurred in meats,
fish, and poultry.  Among the 10 positive composites,  mercury was  detected
in 7 composites but at levels below quantification,  reported as "trace".
The  range of the  positive composites was  from "trace"  to 0.05 ppm  mercury.
Separate analyses of the components of this category revealed seafoods as
the  principal source of mercury.

      In FDA's  Total Diet Survey from FY  1973, virtually all dietary intake
of mercury was  found in the meats,  fish,  and  poultry composite (Mahaffey
et al., 1975).  Mercury was identified in 97  percent of  the  composites,
averaging 0.011 ppm in the composite of meat, fish, and poultry (Table
4.6).  Trace amounts were detected  in a small percentage of  the grains
and  cereals, root vegetables, and oils, fats  and shortening  composites.
Data from Manske  and Corneliussen  (1974),  Mahaffey et  al. (1975),  and
Carroll et al.  (1975) (reporting National  Pesticide Monitoring Program
data) are presented in Table 4.6.   Peyton et al. (1975) assembled  FDA data
which yield the high (26 ppb-Group II) and low  (less than 1  ppb in  many
food groups, 9  ppb in Group II) median values for any  U.S. region  (Table
4.7).  Maximum  reported mercury concentrations for each food  group  range
from 29,400 ppb in red meat to 40 ppb in  dairy products.
ESTIMATED DOSAGE

      Estimated daily intakes of mercury calculated on the basis of FDA
Total Diet Survey data for the years 1971-1974 (Mahaffey et al., 1975) are:

                        1971 -  2.48 yg/day (Goldwater, 1974)
                        1972 -  3.85 yg/day
                        1973 -  2.89 yg/day
                        1974 -  2.84 yg/day (estimated)

      Mahaffey et al. (1975) believe that the estimates presented are
quite accurate for the years 1972-1974 and reflect no trends of increasing
or decreasing mercury exposure through diet for that period.

      Table 4.8 shows the wide variation in dosage of mercury from standard
diets in different age-sex groups.  Children less than 1 year of age receive
the greatest mercury dose on a per weight basis (Peyton et al., 1975).

                                    4-12

-------
                            TABLE  4.6.   MERCURY CONTENT  IN  FOODS:  NATIONAL  PESTICIDES
                                             MONITORING  PROGRAM  MARKET  BASKET  DATA


                                                               Number of    Mean Con-
                                                                Positive    centration,
                  Location     Date3       Food  Class           Composites'3     ppm            Reference               Remarks
                United States   1973   Total diet  study. Positive                        Mahaffey et al.,  1975  Mercury concentrations
                 Sampling               composites were found in                                               reported as total
                 locations              the following categories:                                               mercury.
                 throughout           Food class  II
                 U.S.                   Meat,  fish and poultry              0.011                             Detected in 97% of samples.
                                          composite
                                      Food class  III
                                        Grains and cereals                    Oc                              Detected in 3% of samples.
                                          composite
                                      Food class  VII
                                        Root vegetables                       Oc                              Ditto.
                                          composite
                                      Food class  X
                                        Oil, fats and shorten-                Oc
                                          ing composite
                                      Food class  XII
                                        Beverage  composites                   Oc

                National Totals 1971   Food class  II
 I                                       Meat,  fish and poultry              0.02        Manske and
|_i                                        composite                                       Corneluissen, 1974
U>
                Regions
                f.ali'fornia,    1971   Food class  II
                  Los Angeles            Meat,  fish and poultrv      3                    Carroll et al., 1975   National Pesticides Monitor-
                                          composite                                                            ing Program initiated mercury
                                                                                                              analvsis in October 1970.

                Maryland,      1971   Food class  II                 1
                  Baltimore             Meat,  fish and poultry
                                          compos i to

                Massachusetts,  1971   Food class  II                 .1
                  Boston                Meat,  fish and poultry
                                        compos ite

                Minnesota,     1971   Food class  II                 0                    Carroll er al . . 197^
                  Minneapolis            Meat, fish and poultry
                                          compos ite

                Missouri,      1971   rood i-l.iss  II                 1
                  Kansas Cltv            Meat, fish and poultry
                                          composite



                aD,ites in this  table are I'ise.il years.
                ^Number of positive eomponites out of  i possible 10.
                  I ra

-------
        TABLE 4.7  SUMMARY VALUES FOR MERCURY LEVELS
                   IN NONFISH COMMODITIES
                           (ppb)
                                       FDA
    Type of Commodity
Low    High   Maximumsa
I Dairy product
II Meat, poultry, fish
(composite)
Red meat
Poultry
III Grain and cereal products
TV Potatoes
V + VI + VII (composite)
Vegetables
V Leafy vegetables
VI Legume vegetables
VII Root vegetables
VIII Fruits
IX Sugars, fats, oils
X Beverages
Beer
(<1) 1 3


9 26

(<2) 2 8
(<1) 1 7.5
1 2.5

(
-------
                 TABLE 4.8.   ESTIMATED MERCURY DOSE THROUGH STANDARD DIETS (yg/day)
U.S. per capita
FDA
Type of Commodity Low High Other
I Dairy products 0.44 1.82 3.08
II Meat, poultry, fish 2.90 8.37 24.15
85% CH3Hg+ (2.46) (7.11) 20.52
III Grain and cereal 0.40 1.62 3.02
IV Potatoes 0.17 1.26 0.84
V+VI+VII Vegetables 0.20 0.49 3.37
V Leafy vegetables
I VI Legume vegetables
Ui
VII Root vegetables
VIII Fruits 0.16 0.48 2.74
IX Sugars, fats, oils 0.58 0.58 9.07
X Beverages - Other 0.50 0.50 0.50
Beverages - Beer
Total Hg dose per person,
Pg/day 5.35 14.62 46.77
pg/pg-day
Methylmerdury dose per
person, Mg/day 2.46 7.11 20.52
Mg/kg-day

Weight :
Male a
F
Low
0.89
1.93
(1.64)
0.87
0.18

0.05
0.07

0.16
0.29
0.41
1.20


6.05
0.089

1.64
0.024

67.9 kg
ge: 19
DA
High
2.66
5.56
(4.73)
3.50
1.37

0.23
0.14

0.32
0.87
0.41
1.20


16.26
0.239

4.73
0.070

Weight :
1 ye
FDA
Low
0.80
0.45
(0.38)
0.05
0.01
0.01




0.02
0.04
0.01


1.39
0.180

0.38
0.049

7-7 kg
:ar

High
2.40
1.30
(1.13)
0.20
0.04
0.03




0.05
0.04
0.01


3.89
0.503

1.13
0.146

Weight :
Male agi
Fa
Low
0.70
2.47
(2.10)
0.25
0.09
0.08




0.10
0.29
0.43


4.41
0.680

2.10
0.033

64.5 kg
2: 15-17
4
High
2.10
7.15
(6.08)
1.10
0.67
0.19




0.30
0.29
0.43


12.23
0.189

6.08
0.094

Weight
Female A;
F:
Low
0.48
1.80
(1.53)
0.17
0.05
0.08




0.08
0.19
0.39


3.24
0.058

1.53
0.027

55.
f>e:
DA
H
1
5
(4
0
0
0




0
0
0


8
0

4
0

5 kg
15-1

igh
.44
.20
.42)
.69
.38
.19




.24
.19
.39


.72
.157

.42
.079

Weight
7 Male £

Low
0.28
2.70
(2.30)
0.22
0.08
0.08




0.10
0.26
0.80
0.14

4.66
0.059

2.30
0.029

:: 79.1 kg
ige: 35-54
FDA
High
0.84
7.80
(6.63)
0.90
0.56
0.20




0.30
0.26
0.80
0.14

11.80
0.149

6.63
0.084

Source:  Peyton et al., 1975.

-------
      The U.S. per capita estimated mercury dose in standard diets reported
by Peyton et al. (1975) in Table 4.8 ranges from 5.35 to 14.62 yg/day.
Dietary mercury exposure for the upper 10, 5, 1, and 0.1 percent of the
population in terms of fish consumption is shown in Table 4.9 (Peyton et al.
1975).  Persons belonging to Weight Watchers or others having special diets
which substitute fish for meats are expected to receive above average
quantities of mercury in their diets.  Peyton et al. (1975) have tabulated
these dosage data in Table 4.10.
                                    4-16

-------
        TABLE 4.9.  MERCURY EXPOSURE FROM THE DIET OF FISH EATERS
Fraction of
Population
Exceeding
Grams per day
of Purchased
   Fish
  Mercury Exposure
from Purchased Fisha
Mercury Exposure
from Total Diet
   10%
    5%
    1%
    0.1%
     26
     38
     77
    165
      4.2 g/day
      6.1
     12.3
     26.4
     12.3 g/day
     14.0
     19.9
     33.1
Source: Peyton et al., 1975.
o
 Assumes that the types of fish consumed are the same as for the
   average population in the Market Facts survey.
    TABLE 4.10.  MERCURY EXPOSURE FROM THE DIET OF WEIGHT WATCHERS
                  Source
                   Grams per day
                     Consumed
                     Mercury
                   Exposure, yg
Shrimp
Salmon
Scallops
Tuna
Other fish
Total fish
Other meats
Other food
Total mercury
16.3
16.3
16.3
16.3
32.6
97.8
114.0


1.0
0.7
0.5
5.8
5.2
13.1
1.1
4.6
18.8
               Source: Peyton et al., 1975.
                                   4-17

-------
                             5.   MERCURY IN MAN
      Nordberg  (1976)  has  divided mercury compounds  into  three  functional
categories  on the  basis  of toxic  properties:  (1)  mercury  vapor,  (2)
inorganic mercury  salts  and phenyl-  and  methoxyethylmercuric salts, and
(3) methyl- and ethylmercuric  salts.   This review follows this  same
classification  scheme.
MERCURY VAPOR

      Persons employed  in  the manufacture  of  scientific  instruments,
electrical meters,  alkalies  and  chlorine,  mercury vapor  lamps, amalgams,
solders, and  jewelry  are potentially exposed  to mercury  vapor (Browning,
1969), and concentrations  of mercury vapor occur in laboratories of
chemistry, physics, dentistry, and medicine (Casarett and Doull, 1975).

      Mercury vapor is  absorbed  primarily  through the lungs and respiratory
tract, although  small amounts may also be  absorbed percutaneously  (Nordberg,
1976).  Gastrointestinal absorption after  oral ingestion is minimal.  In
contrast, absorption  of an inhaled dose by the lung has  been estimated as
75 percent complete (Clarkson, 1975).  After  absorption, elemental mercury
is transported by the blood  where it may be either oxidized to the divalent
ion in the red blood  cells and tissues or  may remain dissolved in the
elemental form in the blood  and  transported in this form across the blood-
brain barrier and oxidized later in the brain tissue (Casarett and Doull,
1975).  Most  of  the absorbed mercury is transported within the first few
days to the kidney and  also  to a lesser extent to the liver.  After
repeated exposure, mercury steadily accumulates in the kidneys where it
is bound by sulfhydryl  groups.   At least 1 percent is absorbed by the
brain (Nordberg, 1976).  Experiments in primates as well as human autopsy
findings have shown a differential pattern of distribution within the brain
(Glomski et al., 1973).

      Primate studies suggest that the biological half-life of mercury in
the brain is  probably on the order of years (Nordberg, 1976).  The retention
in the kidney is considerably shorter than that in the brain; however,
retention rates  in the  kidney are not uniform.  Instead, the kidney behaves
as a multicompartmental system;  at least one  compartment shows rather long
retention with biological  half-life exceeding 1 month (Nordberg, 1976).

      These differential retention rates within the several "compartments"
are poorly understood.  Clarkson (1975) has estimated a  whole-body elimina-
tion half-life of 58  days  from a study of  human volunteers who inhaled
radioactive mercury vapor.

                                    5-1

-------
      The primary means of excretion of mercury are  feces  and  urine,  but
smaller amounts may also be eliminated in sweat, milk,  and hair.   At  low
doses, the majority of body burden is eliminated via bile  and  feces.   As
dosage increases, however, a larger proportion is eliminated in  the urine
(Nordberg, 1976).

      No diagnostic index medium  (i.e., blood, hair, urine, etc.)  has been
found which parallels the concentration of mercury in the  brain  from
exposure to mercury vapor.  Neither blood nor urine  accurately reflects
brain levels after exposure to mercury vapor and there  is  no simple
correlation between these parameters (Nordberg, 1976).  Likewise,  there is
no suitable index media to reflect lung levels.

      Approximately 210,000 pounds of mercury per year  are used  in
dentistry.  Each practicing dentist uses, on the average,  2.5  pounds  of
mercury annually in the preparation of dental amalgam restorations.
Because the exposure of the dentist and his assistants  to  this quantity
of mercury suggests the potential for toxicity, Stevens et al. (1972)
conducted measurements of mercury vapor at the Naval Dental Clinic (NDC)
at the Washington Navy Yard, the dental clinic at the Naval Graduate  Dental
School (NGDS), and the staff dental clinic at the National Naval Medical
Center (NNMC).  Atmospheric concentrations of mercury in air at each  of
the facilities were measured throughout the day, at varying distances
from the floor corresponding to the breathing zone of the patient  and
the operator.  Additional measurements were made during vacuuming  in  the
pertinent carpeted areas, trituration of amalgam with the amalgamator,
and on a dental assistant's hands before and after washing.  Urine
samples from assistants working in the room with the highest concentration
(the staff clinic) were compared with control samples from persons working
in the same facility but having no known exposure to mercury.  Table  5.1
shows mercury vapor concentrations in dental operating  rooms at the three
naval dental facilities.  Readings at different heights did not differ
significantly.  Values for all dental operating rooms tested ranged from
a low of 0.001 ppm (0.012 mg/m3) to a high of 0.024 ppm (0.200 mg/m3).  The
highest readings were obtained in the staff clinic during vacuuming of a
carpet in a room where a mercury spill had occurred 4 months previously.
The concentration was recorded at 0.004 ppm (0.035 mg/m3) prior to vacuuming,
peaked to 0.024 ppm (0.1999 mg/m3) during vacuuming, and immediately  decreased
to 0.0096 ppm (0.080 mg/m3) when the vacuuming stopped.  The level remained
above the threshold limit value of 0.0060 ppm (0.05 mg/m3) for a total time
of 25 minutes.  Stamping the foot on the contaminated carpet was followed by
a peak reading of four times the threshold limit value, and peaks of about
twice that amount were recorded when the amalgam was triturated and also
when a drawer containing the amalgamator, mercury bottle, and pellets was
opened.  Following each of these actions, the concentration decreased below
the threshold limit value within 5 seconds.   Urinary mercury levels paral-
leled concentrations in air; the highest mean urine mercury level of 0.0112
ppm was obtained for the three persons working in the room at  the staff
clinic where the mercury vapor in air was also highest.  The observed eleva-
tions in urinary mercury levels are indicative of absorption well in excess
of the normal population.  A study by Gutenmann et al.  (1973) revealed that
89 percent of hair samples obtained from a survey of 115 New York dentists


                                    5-2

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             TABLE 5.1.  MERCURY VAPOR CONCENTRATION IN NAVAL
                         DENTAL OPERATING ROOMS3
Facility
Navy yard, NDC
Clinic, NGDS
Staff clinic, NNMC
Number of
Readings
28
30
34
0
Concentration, pjnn (mg/m )
Mean
0.0031 (0.026)
0.0047 (0.039)
0.0067 (0.056)
Range
0.002-0.0096 (0.017-0.080)
0.001-0.011 (0.012-0.090)
0.003-0.024 (0.022-0.200)
Source:  Stevens et al., 1972.
a                                       ^
 Conversion formula used to convert mg/m  to ppm for air measurements:

                        3 _ ppm  (molecular weight mercury, 200.59)
                          ~               24.04


were above the 0.01 to  2.5 ppm range observed in persons not exposed to
other than "normal" sources of mercury  (Gutenmann et al., 1973).  However,
correlation between number of years in  practice and hair levels was not
significant.

      Smith et al.  (1973) studied  the effects of mercury in 642 workers
exposed to varying levels of mercury vapor in the manufacture of chlorine.
Time-weighted exposure  averages  for mercury in air were determined at
numerous sampling points and the amount of time spent by each employee in
the different locations was specified,  permitting accurate assessment of
individual exposures.   The most  important finding was the existence of a
close correlation (P<0.01) between time-weighted averages of mercury in
air, blood, and urine.  Data from  the study are presented in Figures 5.1
and 5.2.  Such findings suggest  that under chronic exposure conditions,
urinary excretion quite accurately reflects exposure to mercury, at least
on a group basis.  A literal interpretation of the regression lines
indicates that an air concentration of  0.012 ppm (0.1 mg/m ) corresponds
to a blood level of 0.060 ppm and  0.260 ppm in urine.
MERCURIC MERCURY

      Exposure to potentially toxic amounts of mercuric salts occurs almost
exclusively in industrial settings.  In the past, however, ignorance of
the toxic effects of mercuric salts exposed a much wider proportion of the
population, not only through occupational exposures (especially in the
production of felt) but also through medicinal preparations, cosmetics,
and poisons.  Recently, the most common uses of mercuric salts have been
in the paper and pulp industry, in the production of antifouling paints,
and to a lesser extent in the preparation of drugs, disinfectants, and
agricultural products.  Mercuric salts are absorbed by the body primarily
through inhalation and dusts and ingestion, but significant percutaneous

                                   5-3

-------
               E
              O
              o
              x
               o
                 20i-
15
              Ot
              X.
              o
              o
              ffi
                           Oo°
                        0.05   0.10   0.15   0.20  0.25  0.30  O.35
                    Hg AIR LEVELS (mg/m3) Time-weighted averages

           Figure 5.1.  Mean blood  levels of mercury versus  time-
                        weighted  averages of mercury in air
                        (Smith,  1972)*
                           0.05   0.10   0.15   0.20   0.25  0.30
                       Hg  AIR LEVELS (mg/m3) Time-weighted averages

           Figure 5.2.  Urine  levels of mercury versus  time-
                        weighted averages of mercury  in air
                         (Smith,  1972)*
*Reprinted from ENVIRONMENTAL MERCURY CONTAMINATION,  Hartung and Dinman,
    Ann Arbor Science  Publishers, Inc., 1972.
                                    5-4

-------
absorption has  also been  documented  (Casarett and Doull, 1975).  Generally,
where exposure  to  inorganic  dust  occurs,  it  is  in combination with mercury
vapor  (Environmental Health  Resource  Center, 1973).   Inorganic forms of
mercury  taken orally are  not absorbed very quickly by the body.  Only about
2 percent of orally administered  inorganic compounds  is absorbed (Clarkson,
1972).   Once absorbed, approximately  50 percent of the mercury is trans-
ported by the blood plasma and  is rapidly excreted by the urine  (Friberg and
Vostal,  1972).  Following injection of a  mercuric salt in animals, only
about 25 percent of total blood mercury is bound to the erythrocytes.
Comparable values  for mercury vapor were  67  to  68 percent, for arylmercury,
50 percent, and for methylmercury, 90 percent,  bound  to red blood cells.
(Environmental  Health Resource  Center, 1973).   In the blood, the soluble
inorganic mercurial is first attached to  6-globulins  and erythrocytes but
later shifts to albumin from which it is  redistributed to the tissues with
a biological half-life of about 15 days and  is,  therefore, more amenable to
excretion from  the body in urine.  Consequently, less is stored in the
organism following exposure  to  inorganic  salts,  relative to the forms
mentioned above (Friberg  and Vostal,  1972).  The amount and rate of
absorption of aerosols of mercury salts in the  respiratory tract have not
been well established, but is probably less  than that of mercury vapor
(Environmental  Health Resources Center, 1973).   Following acute exposure
to mercuric salts, the highest  tissue concentrations  are found in the kidney
and the  liver.  Inconsistent results  concerning the relative mercury concen-
trations of the other organs have been reported, with most studies showing
minimal  or nonexistent levels in  the  brain (Nordberg,  1976).  Within a few
hours after administration,  the mercury is found in human and animal tissues
in the following approximate order of decreasing concentration: pancreas,
kidney,  liver,  spleen, blood, bone marrow, upper respiratory and buccal
mucosa intestinal  wall (especially colon), skin, salivary glands, heart,
skeletal muscle, brain, and  lung  (Goodman and Gilman,  1975).  There is also
some indication that mercuric salts can be stored in  bones (Goodman and
Gilman,  1975).  Blood levels are  high immediately after exposure, but they
diminish rapidly with time (Environmental Health Resource Center, 1973).
Inorganic mercury  is slowly  released  from tissues, especially the intestines
and muscles (Friberg and  Vostal,  1972).   The favored  excretion route follow-
ing absorption  of mercuric salts  is the urine,  followed by the feces
(Browning, 1969).  Smaller amounts are also  excreted  in the hair, milk,
saliva, and sweat  (Nordberg,  1976).

      Nordberg  (1976) has reported studies on the biological half-life of
inorganic mercury  compounds  in  human  volunteers  given an oral tracer dose.
That estimated  for mercury in blood was 20 days, while that of the whole
body was estimated at about  twice  as  long.   Other studies reported by
Nordberg (1976) have estimated  a  whole-body biological half-life ranging
from 20 to 60 days for human adults.  About  7 percent of the total admini-
stered dose was excreted  during this  time.  Animal experiments suggest that
half-lives in kidney and brain  are considerably longer than those of the
whole body.  Animal experiments also  indicate the possibility that blood
and urine levels may be an appropriate index of  kidney concentration
(Nordberg, 1976).   Regardless of  the  mode of exposure, the kidney is the
critical organ.
                                   5-5

-------
      The organomercurials, methoxyethyl-  and phenylmercury,  are discussed
in this section because their behavior  in  biological  systems  resembles  that
of the inorganic mercury salts.  Both types of  compounds  appear  to  be rapid-
ly metabolized to inorganic mercury, which is then eliminated (Friberg  and
Vostal, 1972).

      Phenylmercuric acetate (PMA) is used as an eradicating  fungicide  and
selective herbicide, especially on golf courses  (Berg, 1976).  Methoxy-
ethylmercurials are also used as fungicides; currently, they  are replacing
methylmercury in many agricultural fungicide preparations  (Nordberg,  1976).

      The absorption and distribution patterns of phenyl-  and methoxy-
ethylmercurials are remarkably similar to  those of inorganic  mercurials
(Casarett and Doull, 1975).  After exposure, both organic  compounds are
deposited in the liver and other tissues where they are converted into
inorganic mercury (Friberg and Vostal, 1972).  Phenylmercurials  are
hydroxylated on the ring, conjugated, and  then decomposed  to  inorganic
mercury and phenol conjugates (Goodman and Gilman, 1975).  The methoxy-
ethylmercurial shows a greater affinity for red blood cells than inorganic,
yet much less than methylmercury.
ALKYLMERCURIALS

      The short-chain ethyl- and methylalkylmercurials have been used to
prevent seedborne diseases in cereal grains, and mercury fulminate is used
in the production of explosives (Browning, 1969).  Methylmercury compounds
are much more readily absorbed by the gastrointestinal tract than inorganic
forms.  Both human and animal experiments have shown 90 to 100 percent
gastrointestinal absorption of methylmercury, while comparable absorption
of inorganic salts is only about 50 percent (Zepp et al., 1974).  Studies
on human volunteers given a single tracer dose of methylmercury revealed
the following metabolic characteristics (Clarkson, 1975):

      (1)  Absorption efficiency equals 95 percent of oral intake.

      (2)  One percent of daily intake was recovered from 1 liter
           of whole blood.

      (3)  Whole body elimination half-life ranged from 52 to 93 days.

      (4)  Half-life clearance in blood was 45 to 105 days.

      (5)  A hair-to-blood concentration ratio of 250:1 was reported.

Methylmercury is well absorbed through the skin and lungs (Casarett and
Doull, 1975).  The absorption of inorganic or organic mercury vapor through
the lungs poses a special problem because vapor exposure produces accumula-
tion of mercury in the brain far in excess of that found after an equivalent
dose of inorganic mercury is administered either orally or intravenously
(D'ltri, 1972).
                                    5-6

-------
      Studies  of  tracer  doses  of methylmercury  showed that the liver and
head together  accumulated  60 percent  of  the  total body burden.  The liver
accounts  for 50 percent  and the head,  10 percent, of the radioactive dose.
Probably  the brain accounts for 90 percent of the head burden (Nordberg,
1976).  Distribution also  differs in  that the levels of mercury in the
kidney, brain, and blood are generally within a two- or threefold range
of each other  after exposure to short-chain  alkylmercurials; differences
observed  with  other types  of mercury  may be  in  the  100-fold range (Casarett
and Doull, 1975).  Methylmercury is preferentially  accumulated in the red
blood cells; more than 90  percent of  methylmercury  present in blood is
bound to  red cells  (Clarkson,  1972).   The superior  biological stability of
alkylmercurials is shown by their resistance to degradation to inorganic
mercury which  can then be  eliminated  from the body.  The 70-day biological
half-life demonstrates slower  excretion  of methylmercury than all of the
other forms (Friberg and Vostal, 1972).   Excretion  of mercury after oral
administration of methylmercury is chiefly in the feces, although smaller
amounts are also excreted  in the urine,  hair, and milk  (Environmental Health
Resource  Center, 1973).  In man, urinary excretion  is slow at first but
accelerates during the first 30 days  after exposure  (Aberg et al., 1969).

      Subcutaneous and intravenous injections produce more rapid excretion
in both urine  and feces; however, the  favored route  for inorganic mercury
is urine, while that for organic is the  feces (Browning, 1969).  Few
comparable data on the metabolism of  ethylmercury are available; however,
it seems  quite similar to  methylmercury  in this respect (Nordberg, 1976).

      A mean biological  half-life for  whole-body clearance ranging from
52 to 93  days  with a mean  value of 76  days was  obtained from tracer studies
of volunteers  given oral doses of 203Hg  (Miettinen,  1973).  Miettinen et al.
(1971) estimated a mean  biological half-life in red blood cells of 50 days,
with a standard deviation  of 7 days.   In a study of persons exposed to
methylmercury  through fish consumption during and after varying degrees of
exposure, Birke et al. (1972)  observed a half-life  of methylmercury of 99 to
120 days  in blood cells, 47 to 130 days  in plasma,  and 33 to 120 days in
hair after correction for  background  levels  in  mercury.  Figure 5.3 shows
decay curves in six subjects after the dietary  change to low fish intake
or to mainly ocean fish, or both.  A methylmercury  biological half-life of
72 days (range 35 to 189 days) was found for the hair of persons who had
ingested  methylmercury-treated grains  (Al-Shahristani and Shihab, 1974).
MERCURY LEVELS IN HUMAN TISSUES

      Mercury levels in tissues of both normal and exposed humans show
considerable variation, but the highest levels are found most  frequently
in the kidney and liver (Environmental Health Resource Center, 1973).
Mercury levels from autopsy tissues  (lung, liver, and kidney)  from  two
geographical areas showed 95 percent of the population having  less  than
3.0 ppm in kidney and 0.2 ppm in liver and 99 percent having less than
1.0 ppm mercury in the lung (Stein et al., 1974).
                                   5-7

-------
   Mercury in
     Hair.
 Blood Cells, and
  Blood Plasma
    (ng gm)
200.000
100.000 -
 50.000 -
 10.000 -
  5.000 -
  1.000 -
   50 -
   10 -
    5 -
    2-I
        9
        ob..
                                  •o
                                                       Subject Tissue (Symbol)
5
1
i
8
3
9
Hair
Han
Hair
Hair
Hair
Hair
(*>
(0)
(.1)
(0)
(A)
(•)
180
,250
65
130
240
88
                                                         1    RBC    (n)     270
                                                	o
8   RBC    (O)     130

1   Plasma   (n)    160
                                                    •O   8   Plasma   (O)    160
      0      200     400     600     800     1,000    1,200    1,400
                       Days After Dietary Change
     Figure  5.3.   Elimination of  total mercury from hair,
                     blood  cells, and plasma  in  six  subjects
                     (Birke et  al.,  1972).

-------
       Table  5.2  summarizes  data  obtained  in  the  United  States between 1970
and  1974.  Over  70  percent  of  assays,  regardless of  organ or age, had a
mercury  burden of less  than 0.25 ppm wet  weight  (Mottet and Body, 1974).
They reported: (1)  no statistically significant  increase in mercury burden
occurred with  age,  indicating  that  past environmental exposures did not
exceed the capacity of  the  body  to  eliminate mercury;  (2) elimination at
present  levels of exposure  equalled intake;  and  (3)  a strong suggestion
that the urban population had  a  somewhat  greater mercury burden than the
rural.   The  authors concluded  that  the general population had a mercury
burden below 1 to 2 ppm in  kidney,  0.25 to 0.75  ppm  in  liver, and less in
other tissues.

       Mercury  was detected  in  76 percent  of  all  autopsy tissues examined
by Gabica et al.  (1975).  The  specimens were obtained from residents of
three geographic regions  of Idaho.   About 3.5 times  more mercury occurred
in kidney tissue than in  liver;  kidney levels were about ten times that
in the brain.  The  highest  levels were found in  samples from persons from
southwest Idaho where natural  mercury  deposits are found.  Glomski et al.
(1973) found a pattern  of selective accumulation within the brain in a
study of persons without  known pathological  exposure to mercury.  As shown
in Table 5.2,  the highest levels were  generally  found in the cerebellar
cortex,  while  the white matter of the  cerebral hemispheres consistently
had  the  lowest or next  lowest  values in all  individuals.

       The upper  limit of  "normal" mercury levels in  urine is 0.20 to 0,25
ppm.   Seventy-five  percent  of  the normal  population  have less than 0.005
ppm  in blood and 95 percent have less  than 0.04  ppm  (Environmental Health
Resource Center, 1973).   Mercury levels in blood, urine, and hair vary
according to exposure.

       Table  5.3 presents  data  from  several studies of hair mercury levels
in normal persons and others with known exposure.  Benson and Gabica (1972)
detected mercury in all 1,000  samples  of  hair from Idaho residents.  Their
study revealed no correlation  between  fish consumption  and other common
sources  of exposure to  the  measured levels.   The mean concentration was
4.18  ppm and mean levels  for males  and females were  2.45 and 5.90 ppm,
respectively.  The  authors  suggest  sexual differences in hair may reflect
hormonal or  biochemical influences.

      Nord et  al. (1973)  sampled hair  from residents of industrialized
Pasadena, California, and the  nonindustrialized  Los  Alamos, New Mexico,
area.  As shown in  Table  5.3,  hair  from women from the  Pasadena area
showed a mean  concentration of 29.6 ppm compared with 20.1 ppm in hair of
women from Los Alamos.  The authors state that their findings are higher
than  other values reported  in  the literature, but they  offered no specific
explanation.   Environmental exposure indices (as measured in dustfall and
house dust)  were significantly associated with values for scalp-hair
mercury  in both children  and adults (Creason et  al., 1975).  Eads and
Lambdins' (1973) study; however,  did not  show significant differences in
mercury  levels in hair  from persons  in a  polluted area  (Port Arthur, Texas)
and literature values.
                                   5-9

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                           TABLE 5.2.   MERCURY CONTKNT OF SELECTED HUMAN  TISSUE  (AT AUTOPSY)
Ul
 I
Location Date
Idaho
Statewide 1973-
1974



b
New York
Buffalo and NA
adjacent Great
Lakes area




Washington (State) ° 1970-
1972









No. of
Samples

28
14
58
36
61
45

6
7
5
7
6
6
7
60
61
57
95
95
77
69
32
60
59
41
Concentration, ppm
Organ and Site

Brain

Kidney

Liver


Brain, frontal lobe cortex
Brain, cerebellar cortex
Brain, medulla
Brain, pons
Brain, midbrain
Brain, thalamus
Brain, white matter
Brain, cerebellum

Heart
Kidney
Liver
Lung
Muscle, unspecified
Pancreas
Skin
Spinal cord
Spleen
Age and Sex

Males, all ages
Females , all ages
Males, all ages
Females , all ages
Males, all ages
Females, all ages

Adults, both sexes
Ditto
11
n
11
11
it
Composite sexes
and ages
Ditto
ti
ii
IT
II
tl
II
II
II
Mean

0.06
0.10
1.06
1.06
0.15
0.53

0.377
0.659
0.354
0.419
0.288
0.323
0.0786
0.132
0.081
0.102
0.757
0.250
0.251
0.126
0.065
0.193
0.087
0.122
Range

0.0-0.25
0.0-0.94
0.0-15.70
0.0-12.50
0.0-2.11
0.0-5.80

0.3-1.69
0.08-1.85
0.04-1.29
0.05-1.66
0.06-1.20
0.04-1.47
0.02-0.15
0.006-0.965
0.008-0.470
0.012-0.704
0.006-6.40
0.008-1.43
0.0-2.763
0.0-0.954
0.007-0.511
0.004-1.275
0.010-0.083
0.012-1.205
           Source: Gabrica et al., 1975.
           'Source: Glomski et al., 1973.
           'Source: Mottet and Body, 1974.

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            TABLE 5.3.  CONCENTRATION OF MERCURY  IN HUMAN HAIR—PERSONS OF LIMITED AND KNOWN EXPOSURE
Ln
1
No. of
Location Date Samples
California
Pasadena 1969 99a
Idaho
Statewide 1971 1,000












New Mexico
Los Alamos 1969 147
80
New York
Central NA 115
Metropoli- 1971 280
tan New 203
York City
Texas
Port Arthur NA 19
19
Concentration, ppm
Age and Sex

Adult, females

Composite sexes and ages
Ages 1-10, male
Ages 1-10 , female
Ages 11-20, male
Ages 11-20, female
Ages 21-40, male
Ages 21-40, female
Ages 41-60, male
Ages 41-60, female
Ages ^61, male
Ages -61, female
All ages, male
All ages, female

Adult , females
Adult, males

Adult, males
Ages 0-15, both sexes
Ages -16, both sexes


Males
Females
Mean

29.6

4.18
2.04
3.21
3.28
6.99
2.01
4.92
2.37
7.64
2.55
6.72
2.45
5.90

20.8
20.1

NA
/H
0.672d
0.774d


5.4
5.5
Range Reference

5.0-410.- Nord et al., 1973

0.12-139.0 Benson and Gabica, 1972
0.26-8.0
0.56-12.0
0.13-107.0
0.25-104.0
0.33-17.6
0.24-43.8
0.20-100.0
0.26-139.0
0.12-24.6
0.64-120.0
0.12-107.0
0.24-139.0

5.0-680.0 Nord et al., 1973
NAc

1.0-34.0 Gutenmann et al., 1973
0.048-11.30 Creason et a , 1975
0.050-14.00


0.2-12.4 Eads and Lambdin, 1973
0.1-139.0
        computation of mean, leaving 98 samples.
      One hundred forty-seven samples were actually taken;  however the  sample  containing  680 ppm was not
        included, leaving 146 samples.
      NA = not available.
     ^Geometric mean.

-------
      The absorption, accumulation, and excretion of methylmercury in man
has been extensively studied in experimental settings as well as in
"natural" exposures such as the epidemics of methylmercury poisoning at
Minamata, Niigata, and Iraq.  Clarkson (1975) has utilized a mathematical
model of methylmercury metabolism to investigate relationships between
exposure levels and various indicator media such as hair, urine, and blood.
Nordberg (1976) has summarized the relationships discovered thus far as
follows:

           •  A specific relationship exists between levels in
              each organ and total body burden of methylmercury.

           •  Definite relationships exist among the levels of
              methylmercury in various organs.

           •  Elimination is correlated with body burden, i.e.,
              a specific fraction of body burden is eliminated
              per unit of time.

           •  A linear relationship exists between daily dose
              and mercury levels in blood and hair.

           •  Levels in blood and hair are related in linear
              fashion, with the hair levels about 250 to 300
              times whole blood levels.  Thus,  hair is of
              greatest potential value as an index media for
              exposure to methylmercury.   Also, hair allows
              recapitulation of past exposure.

           •  At levels below which symptoms of toxicity occur,
              accurate estimation of brain levels may be made
              on the basis of blood levels.

           •  Urine values are of little  value  in estimating
              body burden because not  only are  there low levels
              of methylmercury present in the urine  but  also
              the relatively  larger proportion  of inorganic
              mercury present in urine introduces analytical
              difficulties.
                                    5-12

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                               6.   REFERENCES
 Aberg, B., L.  Ekman,  R.  Fal,  U.  Greitz,  G.  Persson,  and J.-O. Snihs.  1969.
 Metabolism of  Methylmercury  (203Hg)  Compounds  in Man.  Archives of Environ-
 mental Health  JL9_(4) :478-484.

 Al-Shahristani,  H.  and K. M.  Shihab.   1974.  Variation of Biological Half-
 Life of Methylmercury in Man.  Archives  of  Environmental Health 28(6): 342-
 344.

 Anderson, W. L.  and K. E. Smith.   1977.  Dynamics of Mercury at Coal-Fired
 Power Plant and  Adjacent Cooling Lake.   Environmental Science and Technology
 11(1):75-80.

 Andersson, A.  1967-  Mercury  in Swedish Soils.  OIKOS Supplement 9.
 p. 13-14.

 Andren, A. W.  and R.  C.  Harris.  1973.   Methylmercury in Estuarine Sedi-
 ments .  Nature ^45_(5423) : 256-257 .

 Andren, A. W., S. E.  Lindberg, and L.  C. Bate.  1975.  Atmospheric Input
 and Geochemical  Cycling  of Selected  Trace Elements in Walker Branch Water-
 shed.  Oak Ridge National Laboratory,  Oak Ridge, Tennessee.  ORNL-NSF-EATC-
 13.  Environmental  Sciences Division Publication No. 728.  ORNL.  67 p.

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Landner, L.  1971.  Biochemical Model for the Biological Methylation of
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Lee, S. S., B. R. Mate, K. T. von der Trenck, R. A. Rimerman, and D. R.
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                                  6-10

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Lindberg,  S. E. and R. C. Harris.  1974.  Mercury-Organic Matter Associations
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Lucas, H.  F., Jr., D. N. Edgington, and P. J. Colby.  1970.  Concentrations
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Lynch, D.  W.  1973.  Selected Toxic Metals in Ohio's Upland Wildlife.
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Mahaffey,  K. R., P. E. Corneliussen, C. E. Jelinek, and J. A. Fiorino.
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Manske, D.  D. and P. E. Corneliussen.  1974.  Pesticide Residues in Total
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Martin, W.  E.  1972.  Mercury and Lead Residues in Starlings—1970.
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Martin, W.  E. and P. R. Nickerson.  1973.  Mercury, Lead, Cadmium, and
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Marton, J.  and T. Marton.  1972.  Mercury in the Pulp and Paper Mill
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Matti, C.  S., J. P. Witherspoon, and B. G. Blaylock.  1975.  Cycling of
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Mitchell, W. J. and M. R. Midgett.  1976.  Improved Procedure for Determining
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                                    TECHNICAL. F!OJORT DATA
                             (I'lcusr ri'nd Infirm•timn; i.vi the lei'erse before c
 1. IU.POHT NO.
  EPA 560/6-77-031	
 4.TITLL ANDSUI5TITLE
  MULTIMEDIA LEVELS—MERCURY
                                                             G. PERFORMING ORGANIZATION CODE
 7. AUIHOR(S)

  Battelle  Columbus Laboratories
                                          8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
  Battelle Columbus Laboratories
  505 King Avenue
  Columbus, Ohio  43201
 12. SPONSORING AGENCY NAMh AND ADDRESS
  Environmental  Protection Agency
  Office of  Toxic Substances
  Washington, D.C.  20460
                                          3. RECIPIENT'S ACCESSION NO.
                                          !i. REPORT DATE
                                            September 1977
                                          10. PROGRAM ELEMENT NO.
                                          11. CONTRACT/GRANT NO.

                                             68-01-1983
                                          13. TYPE OF REPORT AND PERIOD COVERED
                                          14. SPONSORING AGENCY CODE
 15. SUPPLEMENTARY NOTES
 16. ABSTRACT

  This report is a  review of environmental  levels of mercury based on published reports
  and other information sources.  Mercury levels are reported for the atmosphere,
  surface and ground waters, drinking water,  sediments, soil, terrestrial  and  aquatic
  biota, and man.   The behavior of mercury  in the environment is also discussed.
  Higher than ambient  levels of mercury are found near mercury mines, geothermal  steam
  fields, power plants,  incinerators, sewage  treatment plants, some industrialized
  areas, and indoors where mercury is used.   The release of mercury from natural  sources
  to  air and water  is  double the man-related  losses  to these media.  The levels of
  mercury in biota  are variable, depending  on food habits and environmental conditions.
  The highest levels occur in animals at the  top of  the trophic structure.  Mercury
  levels in tissues of humans are elevated  in chlor-alkali industry workers, in
  dentists,  in people  from areas with natural mercury deposits, and in urban populations
  as  compared with  the rural.
 7.
                                 KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
 Mercury
 Water
 Sediment
 Soil
 Air
 Biota
Human
Food
Behavior
                                               b.IDENTIFIERS/OPEN ENDED TERMS
                                                        c. COSATl rickl/Gump
 8. DISTRIBUTION STATt-MUNT

 Distribution unlimited


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