MIDWEST RESEARCH INSTITUTE
               MRI
EPORT
SAMPLING AND ANALYSIS OF SELECTED TOXIC SUBSTANCES

             Task 2 - Acrylamide



           Contract No. 68-01-4115
           Dr. Vincent J. DeCarlo
       Supervisor, Special Actions Group
         Office of Toxic Substances
     U.S. Environmental Protection Agency
            401 M Street, S.W.
           Washington, B.C. 20460

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MRI-NORTH STAR DIVISION 10701 Red Circle Drive, Minnetonka, Minnesota 55343 • 612 933-7880
MRI WASHINGTON, D.C. 20006-Suite 250,1750 K Street, N.W. • 202 293-3800

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              SAMPLING AND ANALYSIS OF SELECTED TOXIC SUBSTANCES

                              Task 2 - Acrylamide
                            Contract No. 68-01-4115
                            Dr. Vincent J. DeCarlo
                       Supervisor, Special Actions Group
                          Office of Toxic Substances
                     U.S. Environmental Protection Agency
                              401 M Street, S.W.
                            Washington, D.C.  20460
                                 Prepared for

                        Environmental Protection Agency
                          Office of Toxic Substances
                            Washington, D.C.   20460
MIDWEST RESEARCH INSTITUTE  425 VOLKER BOULEVARD, KANSAS CITY, MISSOURI 64110  •  816753-7600

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                                 NOTICE
     This report has been reviewed by the Office of Toxic  Substances,
Environmental Protection Agency,  and approved for publication.  Approval
does not signify that the contents necessarily reflect  the views  and
policies of the Environmental Protection Agency. Mention of trade names
or commercial products is for purposes of clarity only  and does not  con-
stitute endorsement or recommendation for use.

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                                CONTENTS

Section                                                              Page

  I       Summary 	    1

  II      Introduction	    4

  III     Experimental Methods	    6

               Sampling Protocol	    6
               Analysis Protocol	    8
               Method Development for Sampling and Analysis ....   10

  IV      Selection of Sampling Sites 	   24

  V       Discussion of Results	'	   30

               Hercules, Inc., Hopewell, Virginia 	   30
               Calgon Corporation, Ellwood City, Pennsylvania ...   43
               Nalco Chemical Company, Garyville, Louisiana ....   51
               American Cyanamid, Fortier, Louisiana	   58
               St. Regis Paper Company, Sartell, Minnesota	   68
               Dow Chemical, Midland Michigan 	   71
References.
78
                                    ii

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                                FIGURES


No.                               Title                               Page

 1        Vaporization of acrylamide for air sampling studies ....   16

 2        Recovery of acrylamide versus level of acrylamide 	   19

 3        Location of sampling sites	29

 4        Sampling locations at Hercules, Inc., Hopewell, Virginia.  .   34

 5        Mass spectrum of acrylamide	42

 6        Selection ion current plots, m/e = 71.0371	44

 7        Sampling locations at Calgon Corporation, Ellwood City,
            Pennsylvania	46

 8        Sampling locations at Nalco Chemical Company, Garyville,
            Louisiana	54
               -^
 9        Sampling locations at American Cyanamid, Fortier,
            Louisiana	63

10        Sampling locations at St. Regis Paper Company, Sartell,
            Minnesota	72

11        Map of sampling sites for acrylamide at Midland, Michigan .   74
                                   iii

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                                 TABLES
No.                              Title                                Page

 1        Analysis Data Summary for Task 2	     3

 2        BMI's Sampling and Analysis Protocol 	    11

 3        Recovery of Acrylamide Following Evaporation 	    17

 4        Percent Recovery of Acrylamide 	    18

 5        Percent Recovery Versus Volume of Air	    20

 6        Recovery of Acrylamide in Water Following Direct
            Evaporation	    21

 7        BMI's List of Acrylamide Production and User Sites ....    25

 8        Recommended Sampling Sites 	    27

 9        Sampling Schedule	    31

10        Field Sampling Summary 	    32

11        Summary of Air Sampling Parameters	    32

12        Air Sampling Data for Hercules, Inc., Hopewell, Virginia .    36

13        Water and Sediment Sampling Data for Hercules, Inc.,
            Hopewell, Virginia 	    37

14        Weather Conditions During Sampling at Hercules, Inc.,
            Hopewell, Virginia:  Source of Data, Byrd Airport,
            Richmond, Virginia 	    39

15        Acrylamide Concentrations in Air Samples from Hercules,
            Inc., Hopewell, Virginia 	    40

                                    iv

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                           TABLES (continued)
No.                               Title                              Page

16        Acrylamide Concentrations in Water Samples from
            Hercules, Inc., Hopewell, Virginia 	  40

17        Acrylamide Concentrations in Soil and Sediment Samples
            from Hercules, Inc., Hopewell, Virginia	  41

18        Air Sampling Data for Calgon Corporation, Ellwood City,
            Pennsylvania 	  47

19        Water and Sediment Sampling Data for Calgon Corporation,
            Ellwood City, Pennsylvania 	  49

20        Weather Conditions During Sampling at Calgon Corporation,
            Ellwood City, Pennsylvania:  Source of Data, Pittsburgh
            International Airport, Pittsburgh, Pennsylvania	50

21        Acrylamide Concentrations in Air Samples from Calgon
            Corporation, Ellwood City, Pennsylvania	52

22        Acrylamide Concentrations in Water Samples from Calgon
            Corporation, Ellwood City, Pennsylvania	53

23        Acrylamide Concentrations in Soil and Sediment Samples
            from CAlgon Corporation, Ellwood City, Pennsylvania.  .  .  53

24        Air Sampling Data for Nalco Chemical Company, Garyville,
            Louisiana	56

25        Water Sampling Data for Nalco Chemical Company,
            Garyville, Louisiana  	  57

26        Soil Sampling Data for Nalco Chemical Company, Garyville,
            Louisiana	57

27        Weather Conditions During Sampling at Nalco CHemical,
            Garyville, Louisiana:  Source of Data, New Orleans
            International Airport, New Orleans, Louisiana	59

28        Acrylamide Concentrations in Air Samples from Nalco
            Chemical, Garyville, Louisiana 	  60
                                    v

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                           TABLES (concluded)
No.                               Title                              Page

29        Acrylamide Concentrations in Water Samples from Nalco
            Chemical Company, Garyville, Louisiana 	  61

30        Acrylamide Concentrations in Soil Samples from Nalco
            Chemical Company, Garyville, Louisiana 	  61

31        Air Sampling Data for American Cyanamid, New Orleans,
            Louisiana	64

32        Water and Sediment Sampling Data for American Cyanamid,
            New Orleans, Louisiana	65

33        Soil Sampling Data for American Cyanamid, New Orleans,
            Louisiana	  66

34        Weather Conditions During Sampling at American Cyanamid,
            New Orleans, Louisiana:  Source of Data, New Orleans
            International Airport, New Orleans, Louisiana	67

35        Acrylamide Concentrations in Air Samples from American
            Cyanamid, Fortier, Louisiana 	  69

36        Acrylamide Concentrations in Water Samples from American
            Cyanamid, Fortier, Louisiana 	  70

37        Acrylamide Concentrations in Soil and Sediment Samples
            from American Cyanamid, Fortier, Louisiana 	  70

38        Water Sampling Data for St. Regis Paper Company, Sartell,
            Minnesota	73

39        Acrylamide Concentrations in Water Samples from St. Regis
            Paper Company, Sartell, Minnesota	73

40        Analytical Data for Water Samples from Midland, Michigan  .  75

41        Analytical Data for Air Samples from Midland, Michigan  .  .  76

42        Analytical Data for Soil Samples for Midland, Michigan  .  .  77
                                    VI

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                                SECTION I


                                 SUMMARY



     The purpose of this program is to provide sampling and analysis cap-


abilities to Environmental Protection Agency's (EPA) Office of Toxic Sub-


stances, so that the levels of suspected toxic substances in air,  water,


soil, and sediment at designated locations throughout the United States


could be determined. The second task was the sampling and analysis for


acrylamide (AA).


     Methods for sampling and analyzing AA in air, water, soil, and sed-


iments were developed or modified.


     Air samples were collected on Ghromosorb 101 and desorbed for anal-


ysis with methanol. Water samples and methanol extracts of soil and sed-


iment samples were concentrated by evaporation prior to analysis.  All


extracts were analyzed by gas chromatography with nitrogen selective de-


tection. The detection limits of environmental samples varied but  were

                  *\
generally 0.2 y,g/m  for air, 0.8 }j,g/liter for water, and 0.02 M-g/g for


soil and sediment.

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     Five industrial sites were selected for sampling.  Two  plants were




producers of AA, two plants were producers  of AA  and polyacrylamide and




one plant was a polyacrylamide user. A sixth site,  for  which  some results




are reported, was sampled previously by another contractor.




     A summary of the analytical results from the six industrial plants




is given in Table 1. No AA was found in any air samples in  either the




vapor or particulate form. Acrylamide was detected in the aqueous dis-




charge stream for one plant sampled by MRI. The level found was 1,500 ppb




and the identification of AA was confirmed by GC/MS. No AA  was detected




in any of the soil or sediment samples.

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                              Table  1.   ANALYSIS DATA SUMMARY FOR TASK 2
                                             Air  (ug/m5)!?  Water  (yig/^)£/  Soil  (ug/g")g7  Sediment	
             Site                Process      High     Low   High      Low    High    Low    High        Low

Hercules, Inc.                Producer of
  Hopewell, Virginia          polyacrylamide    < 0.4-1.1   1,500   <0.8      <0.08          <0.08

Calgon Corporation            Producer of
  Ellwood City, Pennsylvania  polyacrylamide       <0.1          < 0.8        <0.02          <0.02

Nalco Chemical Company        Producer of
  Garyville, Louisianna       acrylamide and
                              polyacrylamide    < 0.1-0.3        < 0.8        <  0.02          < 0.02

American Cyanamid             Producer of
  Fortier, Louisianna         acrylamide and
                              polyacrylamide       < 0.1         < 0.8        <  0.02          < 0.02

St. Regis Paper Company       User of
  Sartell, Minnesota          polyacrylamide          -           < 0.8

Dow Chemical-
  Midland, Michigan                                < 1.0    25-125   < 25      <  0.02
al  When no acrylamide was detected, the detection limits have been given.
b/  Data from Reference 1.

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                               SECTION II




                              INTRODUCTION







     Acrylamide is the most important chemical in its  group,  being  pro-




duced at an estimated rate of 63 million pounds per year  as of  1976.2.' By




1978, all production should be based on catalytic hydration of  acryloni-




trile. The major producers of AA are American Cyanamid, Dow Chemical,  and




Nalco Chemical. The principal use of AA (~- 80%) is in  the production of




water soluble polymers, commonly called polyelectrolytes, flocculants, co-




agulants, thickening agents, retention aids, and drainage aids. Polymer




production consumes nearly all AA production, much of  it  captively.




     Acrylamide release to the environment occurs at monomer  and polymer




manufacturing sites, polymer application sites, and in transportation  and




handling. Air levels of AA would not be expected to be high due to  its low




vapor pressure. Aqueous discharge is a more likely route  of release be-




cause of its extremely high water solubility and the major use  of poly-




acrylamides in aqueous systems, e.g., flocculants, grouts. The  only avail-




able monitoring data for the discharge of AA into the  environment is  from

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Croll's study in Great Britain during which levels as  high as  42 |j,g/liter




      K     A 3/
were observed.~




     The environmental concern about AA results principally from its  re-




ported neurotoxicity. The acute LDcg in rodents—  is 200  mg/kgj however,




lower dosages have shown a cumulative toxicity.—'  The  carcinogenicity of




AA is unknown.




     In 1976, MRI Project No. 4280-C, entitled "Sampling  and Analysis of




Selected Toxic Substances" was initiated. The objective of this program




is to provide the EPA with sampling and analysis capabilities  to determine




the levels of selected toxic substances in air, water,  soil, and sediments




from designated sources and ambient locations throughout  the United States.




The first task on this program, vinylidene chloride, has  been  completed.




The second task on the program, which is the subject of this report,  is




AA. A requirement of the program is the development of a  sampling  and anal-




ysis protocol that is sensitive and reliable at the low parts  per  billion




range. Appropriate industrial sites are then selected and monitored for




AA using the protocol.




     The remainder of this report describes the experimental methods  em-




ployed, including the development of the sampling and analysis protocol,




the selection of sampling sites, and a discussion of the  monitoring re-




sults.

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                               SECTION III




                          EXPERIMENTAL METHODS







SAMPLING PROTOCOL




Air Sampling




     Air was sampled at four to five stations deployed upwind,  downwind,




and crosswind of the industrial sites. Duplicate samplers  were  placed  at




some of the stations. The sampling probes were set at  1.5  m above  ground




and air was sampled at 0.5 to 1 liters/rain continuously for up  to  24 hr.




     The meteorological conditions that existed during each test were  ob-




tained from the nearest National Weather Station. Wind speed and direc-




tion, temperature, and precipitation were included in  the  data  log.




     The sampling train for the collection of AA from  ambient air  con-




sisted of the following components assembled in the order  given:   (a)




Millipore filter (AAWP03700), directed into the wind;  (b)  top Chromosorb




101 tubej (c) bottom Chromosorb 101 tube; and (d) 24-v DC  vacuum pump.




     The adsorbent tubes were constructed of glass and were 8 mm OD  x  6 mm




ID x 18 cm in length. Prior to use the internal surfaces were silanized.

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The tubes were packed with 0.7 g 60/80 mesh Chromosorb  101 that had been




Soxhlet extracted with methanol overnight  and then  dried  at  150°C for 4 hr




under a stream of nitrogen. Minimum lengths of natural  tubing were used




to connect the sampling tubes to the pumps. No critical orifice was used




as the pressure drop of the two tubes was  sufficient  to keep the flow rate




under 1 liters/min. The actual flow in the field was  monitored by cali-




brated rotameters every 3 to 4 hr. Power was supplied to  the DC pump by




two 24-v lantern batteries connected in parallel.  The pump and batteries




were encased in a cardboard-polystyrene shipping box  which was then an-




chored at the sampling site. The reliability of this  system  has been dem-




onstrated in previous OTS studies••2*I/ After sampling was completed, the




tubes were capped with rubber slip-on septa, wrapped  in aluminum foil and




stored over dry ice for shipment to MRI. At MRI, the  tubes were kept on




dry ice until analyzed.




Water Sampling




     Grab water samples at the outfalls were collected  periodically dur-




ing the air sampling period and composited in 1 gal.  brown silanized bot-




tles with Teflori®-lined caps. Upstream and downstream samples were collected




concurrently. After collection the samples were placed  on wet ice for ship-




ment to MRI. At MRI, the samples were stored at 2  to  4°G.




Soil Sampling




     Soil samples from the top 10 mm were  collected at  the air sampling




stations and placed in 16-oz jars with aluminum foil-lined caps. The jars

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were then stored over dry ice for shipment to MRI. At MRI,  the samples




were kept on dry ice until analyzed.




Sediment Sampling




     Sediment samples were collected by use of an Eckman dredge. The sam-




ples were placed in 16-oz jars with aluminum foil-lined caps. After col-




lection the samples were placed and shipped on wet ice to MRI. At MRI, the




samples were stored at 2 to 4°C.




ANALYSIS PROTOCOL




Air Samples




     The Chromosorb 101 tubes were inverted and eluted with 10 ml of meth-




anol. The methanol extract was then reduced in volume to approximately 0.5




ml by evaporation with a nitrogen stream. The Millipore particulate fil-




ters were extracted for 0.5 hr in an ultrasonic bath with 2.0 ml water.




     The extracts were analyzed by GC with electrolytic conductivity




(Hall) detection. The operating parameters were as follows:




     Column:  1.8 m x 2 mm ID silanized glass




     Packing:  80/100 mesh Chromosorb 101




     Carrier:  Helium at 30 ml/rain




     Reaction tube:  Quartz with Ni catalyst, hydrogen at 35 ml/min




     Electrolyte:  157= isopropanol/water, 0.5 ml/min




     Injector temperature:  240°C




     Column temperature:  220°C




     Reaction furnace temperature:  820°C

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A limited number of sample extracts were spiked with a known  quantity  of




AA and reanalyzed. The results were used to establish limits  of  detection.




Water Samples




     A 250-ml aliquot of the water sample was reduced in volume  to  about




10 ml by rotary evaporation. The sample was then further reduced in vol-




ume to 0.5 to 8 ml using a nitrogen stream. Any solid which formed  dur-




ing the evaporations was removed by centrifugation and extracted with




methanol. The two fractions were analyzed separately by GG using the con-




ditions outlined above. A limited number of samples were spiked  with a




known amount of AA and reanalyzed in order to establish the detection




1 imi t s •




Soil Samples




     Fifty-gram aliquots of the soil samples were extracted twice with




50 ml portions of methanol using ultrasonic agitation. The extracts were




combined and evaporated to 5 ml. Any solid which precipitated was removed




by centrifugation. Separate soil samples were dried at 110°C for 4  days




in order to determine the moisture content. The sample extracts  and spiked




sample extracts were analyzed by GC using the conditions outlined above.




Se diment Samp 1e s




     Sediment samples were first centrifuged to separate the water  which




was then removed. A 50-g sample was then prepared and analyzed in the  same




manner as the soil samples.

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GC/MS Confirmation



     Samples were confirmed by GC/MS using the same column  and conditions



described above. In order to eliminate a column bleed problem,  the MS was



operated in a high resolution selected ion monitoring mode. Although only



one mass was monitored, the selectivity provided by high resolution MS



virtually eliminated all compounds except a CoHj-NO. The matching  of reten-



tion times then confirms the presence of AA. The instrument operating con-



ditions were as follows:
                                                      0


     Instrument:  Varian MAT 31lA mass spectrometer with Varian 2700 gas



                    chromatograph



     Data system:  Varian Spectrosystem 100 MS



     Separator:  Watson-Bieman



     Mass monitored:  71.0371



     Resolution: ~ 7,000



     lonization potential:  70 ev



     Emission current:  1 mA



     Multiplier gain:  4 x 106 - 2 x 107



METHOD DEVELOPMENT FOR SAMPLING AND ANALYSIS



     A protocol for sampling and analysis of AA was under development by



Batelle Memorial Institute (BMI). The techniques which were proposed and



used at one site are summarized in Table 2. Also included is the extent



to which the method was validated. In discussing the protocol with OTS
                                    10

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                           Table 2.  BMI'S SAMPLING AND ANALYSIS PROTOCOL^'
                                                        I/
Media

Air

  Vapor
  Particulates
Water
         Sampling technique
Midget impinger with 35 ml of
  40% methanol-water.  Sample
  1-1.5 1pm for 24 hr.  Store
  in a refrigerator.

Extract 3 times for 10 min with
  7 ml methanol.
Grab sample of 1 liter.
Sample treatment and preparation
Evaporate to 1 ml.  Analyze as a
  water sample by direct
  injection.
Evaporate to 0.5 ml or less.
  Analyze as a water sample by
  direct injection.

Evaporate 20 ml to 0.5 ml or less
  by Kuderna Danish or slow nitro-
  gen stream with heat.  Analyze
  by direct injection.
Verification
or recovery
None reported.
None reported.
10 ml of 1 ppm
  evaporated
  and reconsti-
  tuted gave
  total recov-
  ery in silyl-
  ated vessels.
Soil
Dry and extract a 50-g sample
  with 70 ml methanol.  Repeat
  and filter the combined
  extracts.
Evaporate to 0.5 to 1 ml.  Analyze
  as a water sample by direct
  injection.
None reported.

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personnel, it was decided that some modifications might be worth investi-




gating. The development of MRI's final protocol is discussed below.




GC Analysis Conditions




     The procedure used by BMI was based on direct injection of the  aque-




ous or methanolic sample onto a Chromosorb 101 column. A nitrogen sensi-




tive thermionic detector was used to achieve the necessary selectivity for




AA in the presence of hydrocarbons. Initially, the acrylamide analyses at




MRI were performed on a Perkin-Elmer Model 3920 instrument equipped  with




a nitrogen/phosphorus selective flame ionization detector. A 5 ft x  1/8




in. OD glass column packed with 80/100 mesh Chromosorb 101 was used  with




a helium carrier flow rate of 20 ml/min. The operating conditions were:




injection temperature, 220°C; column temperature, 220°C; transfer line/




detector temperature, 250°C; hydrogen flow, 3 ml/min;  air flow, 65 ml/




min. At these conditions AA elutes in 3.8 min. The sensitivity of the de-




tector was monitored periodically by measuring the bead current. A con-




stant bead current of 30 picoamperes was maintained by adjustment of the




bead heating current with a jet potential control setting of 3. However,




because of difficulties in maintaining sensitivity levels on that system,




it was decided to investigate a different detection system. A Varian 3700




gas chromatograph equipped with a Hall Electrolytic Conductivity Detector




(Tracor, Inc.) in the nitrogen mode was investigated.  A 6 ft x 2 mm  ID glass




column packed with 80/100 mesh Chromosorb 101 was used with a helium car-




rier flow rate of 30 ml/min. Other operating conditions were:  injector
                                    12

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temperature, 240°C; column temperature, 220°Cj  detector base temperature,




240°C; detector furnace temperature, 820°C;  hydrogen reactor gas  flow,  35




ml/min; 157, isopropanol/water electrolyte flow rate, 0.5 ml/min.  Under




these conditions, AA eluted in 5.1 min. A calibration curve using AA  in




methanol is shown in Figure 2. The sensitivity and stability of the Hall




detector appear to be better than that of the nitrogen/phosphorus selec-




tive AFID.




     This system was then used for most of the method development studies




and all of the sample analyses. In operation, the solvent was vented  for




3 to 4 min following sample injection.




Air Samples




Recovery from Charcoal - The sampling method proposed but untested by BMI




used water filled midget impingers that were preceded by a glass  fiber  fil-




ter. It was agreed at the project meeting that the impinger train had sev-




eral disadvantages and that it would be worth investigating the use of




solid adsorbents, e.g., charcoal, as the trapping medium. Accordingly,




our work concentrated on evaluating several alternate air sampling trains.




     In the first set of experiments, activated charcoal was tested as  an




adsorbent. The charcoal, Fisher Coconut charcoal, 8/15 mesh, was  extracted




with methanol and activated at 100 or 250°C under a flowing stream of ni-




trogen for 1 hr. A preliminary recovery experiment was made by packing  two




glass tubes, 8 mm OD x 6 mm ID x 60 mm length, with about 1 g of  charcoal




activated at 100 or 250°C. Acrylamide, 100 pi of 1 ppt in methanol, was




injected onto the top of the two columns. The tubes were eluted directly






                                   13

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with methanol and successive 4-ml aliquots were collected for analysis.




In both cases, the AA that was desorbed completely eluted in the first  12




ml of methanol. The total recoveries were 57% and 30% for charcoal  acti-




vated at 100 and 250°C, respectively. The results suggested that AA was




being irreversibly adsorbed on highly reactive sites and that the high




temperature charcoal had more reactive sites. A reverse desorption  exper-




iment was made by adding 5 ml of 10 ppm AA in methanol to 2 g of the 100°C




charcoal. The solution level of AA was monitored periodically for 40 min.




At that time, the "free" AA had dropped from 100 to 62%, indicating that




the charcoal was actually adsorbing AA from the methanol. Thus,  while




charcoal might be a good trap for AA, the low recovery by methanol  elu-




tion encouraged the search for another adsorbent.




Recovery from Tenax® and Ghromosorb 101 - The same reverse desorption




experiment was repeated using 80/100 mesh Chromosorb 101 and 60/80  mesh




Tenax®-GC. After 40 min, the "free" AA was 93 and 98%, respectively. An-




other set of experiments with the glass tubes was run, using charcoal




(100°C activation), Tenax® and Chromosorb 101. The tubes held about 1,  0.6,




and 0.7 g of adsorbent, respectively. Each adsorbent was spiked with 100




|jil of 1 ppt AA in methanol and air was drawn through at 0.5 liter/min for




30 min. The tubes were inverted and eluted with two 10-ml aliquots  of meth-




anol. The recoveries were  65, 110 and 90%, respectively, and all the AA




was found in the first 10 ml of methanol.
                                   14

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     A related experiment was then performed in an attempt  to  transfer


the AA to the adsorbent as a vapor rather than a solution.  Acrylamide was


vaporized using the apparatus shown in Figure 1.


     Air was pulled through the system for 1 hr at 0.5 liter/min. The in-


side temperature of the U-tube was measured at ~ 100° C. Acrylamide was  re-


moved from the Chromosorb 101 by elution with 10 ml methanol and an 83%


recovery was achieved. This level of recovery was felt to be within exper-


imental error of the 9070 recovery achieved following the direct  application


of the AA in methanol. Subsequent recovery experiments were done by direct


injection of AA in methanol onto the adsorbent. Chromosorb  101 has been


tentatively chosen over Tenax® for the sampling train for reasons of avail-


ability and cost.


     In the next experiment, Chromosorb 101 was spiked with 200 p,g AA and


air was drawn through the tube for 16 hr at 0.6 liters/min. After elution


with 10 ml methanol, a 90% recovery of AA was found. Additional  experiments


run with longer sampling times and higher flow rates are discussed below.


Evaporation of Methanol Extracts - The size of the adsorbent traps being


evaluated required 10 ml of methanol for quantitative elution. The possi-

               ^
bility of reducing this volume to 1 ml or less by evaporation was investi-


gated. The objective of the study was to lower the air level limit of  de-


tection by increasing the analyte concentration through evaporation. Four


25-ml aliquots of 10-ppm acrylamide in methanol were evaporated to 1 ml


at 25 and 50°C in silylated and unsilylated vials, under a stream of ni-


trogen. The recoveries are given in Table 3.



                                   15

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                                    Vacuum Pump
                      Pyrex Tubing
Hoi- Air Gun
                                        t
                                                Glass Wool
                                                Chromosorb 101
•Glass Wool




•Teflon Coupling
Acrylamide in Methanol
Figure 1.  Vaporization of acrylamide  for  air  sampling  studies
                               16

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       Table 3.  RECOVERY OF ACRYIAMIDE FOLLOWING EVAPORATION






     Temperature         Silylated vial         Unsilylated vial




        25° C                  837,                     74%




        50° C                  83%                     83%







Thus solutions of AA in methanol can be effectively evaporated at  50°C  in




silylated or unsilylated glassware. As a precaution, however, silylated




glassware was used routinely*




Recovery at Various Loading Levels - To determine the percent recovery  at




various levels of loading, 2.9 to 214 ^g of AA was loaded by injection




onto 0.7 g 60/80 mesh Chromosorb 101 packed into silanized glass tubes.




The larger mesh adsorbent was used to reduce the pressure drop across the




sampling tube. Each tube was connected to a small pump and air was drawn




through the tube at a rate of 325 to 450 ml/min. After 24 hr, each tube




was inverted and eluted with 10 ml methanol. The eluates from the  tubes




loaded with 100 to 200 (j,g AA were analyzed directly. The eluates from the




tubes loaded with 2.9 to 29 y,g AA were evaporated from 10 ml volume to




approximately 1 ml using a 50°C water bath and a gentle stream of  nitrogen.




In order to calculate the exact volumes of the evaporated samples, a known




amount of benzonitrile in methanol was added to each. The samples  were  then




analyzed and quantitated using a mixed AA-benzonitrile standard. The per-




cent recovery for each sample was determined and is listed in Table 4.




The data are also graphed in semi-log form in Figure 2. The four points




were averaged to obtain a 99% recovery level.






                                   17

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           Table 4.  PERCENT RECOVERY OF ACRYLAMIDE
Acrylamide         ^g Acrylamide         Percent
 loaded              recovered           recovery         Average

  2.90                   3.01             104              104%
 29.0                   38.7              133 )
 29.0                   39.7              137 >           121%
 29.0                   26.6               92 )
107                     96.0               90              90%
214                    156                 73 )
214                    171                 80 >            77%
214                    168                 79 )
                               18

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200  300
1000
Figure 2.  Recovery of acrylamide versus level of acrylamide

-------
Recovery at Various Air Volumes - An experiment was then performed to de-

termine the effect on recovery of the volume of air sampled in a 24-hr

period. Pour silanized glass tubes packed with 0.7 g Chromosorb 101 were

loaded with 214 (j.g AA by injection of a methanolic standard. Each tube

was connected to a small vacuum pump and air was drawn through at a rate

of 250 to 600 ral/min. At the end of the 24-hr period, each tube was in-

verted and eluted with 10 ml methanol and analyzed. The percent recovery

was determined and is listed in Table 5.


            Table 5.  PERCENT  RECOVER?  VERSUS  VOLUME OF AIR	
          yg  Acryl-   yg  Acryl-
            -amide       amide       Air  flow      Total air    Percent
  Sample     loaded     recovered    rate (ml/min)   volume  (£)   recovery
1
2
3
4
214
214
214
214
168
171
156
123
250
370
380
600
360
540
600
880
79
80
73
57
     It appears that the percent recovery is decreasing at the higher

flow rate. It was concluded that air sampling should be done at 500 ml/

min or less for best recovery. If a high flow rate is desired, the use of

a backup tube is mandatory.

Water Samples

Recovery from Charcoal - The procedure first proposed by BMI-=-' was based

upon the adsorption of AA from water with a column of activated charcoal.

It was reported that 1,000 ml of water, 1 ppm in AA, could be passed

through 2 g of petroleum-based charcoal (30/60 mesh, SKC, Inc.) and the
                                   20

-------
AA eluted with 20 ml methanol with a 76% recovery. A greater  concentra-

tion factor could be achieved by evaporating the methanol  to  1 ml  or  less.

     The experiment was repeated at MRI with less success. Two grams  of

the Fisher charcoal activated at 100° C were packed into a  column and  1,000

ml of water, 1.07 ppm in AA, was passed through at 10 ml/min. The  charcoal

was dried with a nitrogen gas stream and eluted with 25 ml methanol.  Only

16% of the expected AA was recovered. It was not established  whether  the

low recovery was due to breakthrough or incomplete desorption with metha-

nol.

Direct Evaporation - It was also reported by BMlI/ that aqueous AA solu-

tions could be preconcentrated by direct evaporation.

     Several direct evaporation experiments were conducted using conven-

tional rotary evaporator equipment. Increasing volumes of  water with  de-

creasing AA concentrations were evaporated with a 50° C heated bath, to

less than 1 ml. At the higher levels, the concentrate was  diluted  to  10.0

ml and analyzed directly. At the lower concentrations, a known  amount of

benzonitrile was added to the ~ 1 ml of concentrate as an  internal stan-

dard. The percent recoveries of the low level samples are  given  in Table

6.
                Table 6.  RECOVERY OF ACRYLAMIDE IN WATER
                      FOLLOWING DIRECT EVAPORATION

Original
volume (ml)
100
100
1,000
Acrylamide
(ppm)
1.00
0.100
0.010
Final
volume
10.0
2.2
0.89
Percent
recovery
92
74
71
                                    21

-------
     It was concluded that direct evaporation could be used on  the  real




samples with an acceptable recovery.




Soil Samples




     Two 10-g samples of Kansas City garden soil were spiked with 107 |j,g




of AA. The first was packed into a chromatography column and eluted with




methanol. Five successive 5-ml aliquots were collected and analyzed. The




first 5-ml fraction was found to contain all of the added AA. The second




10-g sample was covered with 10 ml methanol in a vial and sonicated for




5 min. The sample and methanol were then centrifuged and the extract re-




moved and analyzed. The recovery of AA was 75%. An unspiked 10-g  sample




was also eluted with 5 ml methanol and found to contain no AA.




     For analysis of field soil samples, an elution with methanol will be




used*




Sediment Samples




     The sediment sample collected from the Calgon site was used  for the




development of the sediment analysis protocol. Six sediment samples of




approximately 10 g each were centrifuged and the supernatant water  was




removed. Four of the samples were spiked with 100 y,g of AA. Two of  the




spiked samples were transferred to columns and eluted successively  with




three 5-ml aliquots of methanol. The total recoveries of AA from  the sam-




ples were 83 and 65%.




     The other two spiked samples were sonicated 1 hr with 10 ml  methanol.




In this case, 76 and 81% of the added AA were recovered. No AA  was  recov-




ered from the unspiked sediment using either elution technique.







                                   22

-------
     The sonication technique will be used for the analysis  of  real  sam-




ples due to its experimental simplicity and slightly  better  precision. Ap-




proximately 50 g of sediment will be sonicated successively  with  two 50-ml




aliquots of methanol. The methanol extracts will  be combined and  evaporated




to 5 ml prior to analysis.
                                   23

-------
                               SECTION IV




                       SELECTION OF SAMPLING SITES







     A meeting was held at the Office of Toxic Substances,  EPA,  in  June




1977 to discuss the selection of sampling sites. Present  were Dr. J.




Hillman and Dr. P. Hilgard of OTS, and Dr. J. Going from  MRI.




     Table 7 lists the production and user sites that  had been proposed




for sampling by BMI. Standard Oil of Ohio was eliminated  as it no longer




produces AA. American Cyanamid, New Orleans, was eliminated based on  the




remarks of Dr. Bikales, National Science Foundation,  that the plant would




be difficult to monitor because of its location. It was suggested that




Du Pont probably uses AA as a thickening agent for gelled explosives.




     Table 7 also lists the user sites proposed by BMI. Betz was estab-




lished to be a minor user and Celanese no longer uses AA. The paper mills




were eliminated on the suggestion of Dr. Bikales.




     With the assistance of Bob Peterson of MRI's North Star Division, ad-




ditional information was gathered about the producers and users of  AA. A




new list of recommended sampling sites was developed and  discussed  with
                                   24

-------
       Table 7.  BMI'S LIST OF ACRYLAMIDE PRODUCTION AND USER SITES-
                                                                   II
Acrylamide production sites

Dow Chemical, USA, Midland, Michigan
DuPont, Belle, West Virginia
Bio-Rad Laboratories, Richmond
  California
American Cyanamid, Linden,
  New Jersey
American Cyanamid, Fortier
  Louisiana
Standard Oil of Ohio, Lima, Ohio

Acrylamide user sites

Hercules, Inc., Hopewell, Virgina
Nalco Chemical Company, Chicago,
  Illinois
Calgon Corporation, Pittsburgh,
  Pennsylvania
Pascagoula, Mississippi
  (soil stabilization)
Wenatchee, Washington
  (soil stabilization)
Betz Laboratory, Trevase,
  Pennsylvania
Celanese, Charlotte,
  North Carolina
Kimberly-Clark, Appleton,
  Wisconsin
Mead Paper, Chillicothe, Ohio
Recommended production sites

Dow Chemical, Midland, Michigan
DuPont, Belle, West Virginia
Bio-Rad Laboratories, Richmond,
  California
American Cyanamid, Linden,
  New Jersey
Recommended user sites

Hercules, Inc., Hopewell, Virginia
Nalco Chemical Company, Chicago,
  Illinois
Calgon Corporation, Pittsburgh,
  Pennsylvania
Soil stabilization site to be
  specified
Soil stabilization site to be
  specified
                                    25

-------
J. Hillman and P. Hilgard of OTS. This list, given as Table 8,  differs  in




the following ways.




     The Dow Chemical plant at Midland,  Michigan,  had been sampled by EMI




and was eliminated. The Bio-Rad plant is a minor producer and user and  is




unlikely to have significant emissions of AA. The American Cyanamid plant




at Linden, New Jersey, was not recommended for several reasons. The plant




will be switching to the catalytic conversion of acrylonitrile as the pro-




duction process by 1978. The physical location of the plant makes off-




plant air sampling extremely difficult.  Furthermore,  the aqueous wastes




are barged out into the Atlantic Ocean and dumped.




     It was recommended that the American Cyanamid plant at Fortier,




Louisiana, be sampled instead. It is representative of the most commonly




used production process and is both a producer and a user. It was also




recommended that the Nalco plant at Garyville, Louisiana, be sampled as




a producer and a user. This plant was not on the BMI producers list.




Among the polymer producers, Nalco at Garyville (as a monomer and poly-




mer producer) rather than at Chicago, Illinois, was recommended.




     A paper manufacturer, in the category of a polymer user, was added




to the recommended list. The St. Regis plant at Sartell, Minnesota, would




be monitored for aqueous discharges only.




     The Hercules plant in Hopewell, Virginia, the Calgon plant in




Pittsburg, Pennsylvania, as well as a soil stabilization site, were recom-




mended by both MRI and BMI. In the course of attempting to locate a suit-




able soil stabilization site, MRI was advised by Mr. David Withers,







                                   26

-------
                                       Table  8.  RECOMMENDED SAMPLING SITES
to
—I
         Site
                          Location
      Monomer and polymer  producers

      American Cyanamide   Fortier, Louisiana
      Nalco
                     Garyville, Louisiana
Polymer producers

Calgon               Pittsburgh, Pennsylvania
      Hercules



      Polymer users

      St. Regis
                     Hopewell, Virginia
                     Unidentified
                     Sartell, Minnesota
   Category
Monomer producer
Polymer producer

Monomer producer
Polymer producer
Polymer producer

Polymer producer

Soil stabilization



Polymer user
Type of operation/process, comments
                                                                      Catalytic conversion of acrylonitrile
Catalytic conversion of acrylonitrile
Manufacture of paper

-------
Regional Manager, Mining and Industrial Chemicals,  American Cyanamid,




that he was not aware of any suitable ongoing projects.




     The locations of the sampling sites are shown  in Figure 3.
                                   28

-------
D  Acrylamide, Polyacrylamide Producer
A  Polyacrylamide Producer
O  Polyacrylamide User
                               Figure 3.  Location of sampling sites

-------
                                SECTION V




                          DISCUSSION OF RESULTS







     The five selected industrial sites were sampled between August  8,




1977, and October 6, 1977. The actual sampling schedule  is  shown  in  Table




9; presampling site visits were generally conducted on the  day  prior to




sampling. A summary of the air and water samples collected  at the five




sites is given in Table 10 and a summary of the air sampling parameters




follows in Table 11. The results obtained from the five  industrial sites




are discussed below. A sixth site was sampled previously by another  con-




tractor and these results are also given for comparison.




HERCULES, INC., HOPEWELL, VIRGINIA




Field Sampling




     A presampling survey was conducted on August 8, 1977.  The  plant lies




within the city limits of Hopewell, Virginia, south of the  junction  of




state highways 10 and 156. Allied Chemical operates a large complex  ap-




proximately 0.75 miles to the north-northeast of the Hercules plant. Ap-




proximately 0.75 miles to the northwest is a Firestone polymer  plant. The
                                   30

-------
                                     Table 9.  SAMPLING SCHEDULE
     Date

8/8/77-8/10/77

8/30/77-8/31/77

9/12/77-9/14/77
        Company

Hercules, Inc.

Calgon Corporation

Nalco Chemical Company
9/14/77-9/16/77    American Cyanamld
         Location

Hopewell, Virginia

Ellwood City, Pennsylvania

Garyville, Louisiana


Fortier, Louisiana
 Product or operation

Polyacrylamide producer

Polyacrylamide producer

Acrylamide producer
Polyacrylamide producer

Acrylamide producer
Polyacrylamide producer
10/6/77
St. Regis Paper Company
Sartell, Minnesota
Polyacrylamide user

-------
                   Table 10. FIELD SAMPLING SUMMARY

Site
Hercules



Calgon


Nalco


American Cyanamid


Air samples Total samples
(stations x components) (number/type)
5x2 10 Air
4 Soil
4 Water
1 Sediment
4x2 8 Air
4 Soil
4 Water
1 Sediment
4x2 8 Air
3 Soil
3 Water
4x2 8 Air
4 Soil
3 Water
2 Sediment
St. Regis

            a/
Dow Chemical—
                                     2 Water
a.1  Samples collected and analyzed by separate contractor.  See Ref.  1,
              Table 11.  SUMMARY OF AIR SAMPLING PARAMETERS
       Site

Hercules
Calgon
Nalco
American Cyanamid
Dow Chemical^'
 Average sample
   volume (j0)
(no. of samples)

     402 (5)
   1,008 (4)
     722 (4)
     893 (4)
   Average
sampling time
    (hr)

    25.9
    25.5
    20.1
    23.4
   Average
sampling rate
   (&/min)

    0.26
    0.66
    0.60
    0.64
_§/  Samples collected  and  analyzed by  separate  contractor.  See  Ref.  1.
                                   32

-------
municipal sewage treatment facility is located in the city landfill im-




mediately south of the plant adjacent to Bailey Creek into which it dis-




charges.




     The NPDES permit application shows four discharge points,  001 through




004, which are labeled accordingly in Figure 4. All four flow into Bailey




Creek which then discharges into the James River. These discharges receive




some treatment before being discharged. At the present time a regional




sewage treatment facility is nearing completion at the junction of Bailey




Creek and the James River. We were informed by Mr. Burnett of the State




Water Control Board that the facility is being funded by the industrial




sites, the City of Hopewell, and the U.S. Environmental Protection Agency




and when completed will receive treated waste from the city and each in-




dustrial site. Discharge into Bailey Creek from Hercules will still occur




during heavy rains.




     The Hercules plant manufactures various celluloses, chlorine, chlo-




roacetic acid, ethyl ether, hydrochloric acid, sodium sulfate,  and poly-




acrylamide.




     Local winds were reported to come primarily out of the southeast at




this time of year.




     Field sampling was carried out during the period from 0900 August 9,




1977, to 1300 August 10, 1977. Five air samplers were deployed at four




sites upwind, downwind, and laterally with respect to the plant. Soil sam-




ples were taken from the air sampling sites, water samples were taken
                                   33

-------
                       rPT-.-VXb,. ^ \-.^r/f •*:-
                                            ^^
                                                    U '•  -vfe^-
Figure 4.  Sampling  locations  at  Hercules, Inc., Hopewell, Virginia
                                  34-

-------
upstream and downstream from the point of discharge, and a sediment sample




was taken below an outfall believed to be No. 004.




Air Sampling - Air sampling was conducted using five samplers at four sites.




The sites were established upwind, downwind, and laterally with respect to




the plant with two samplers located at the downwind site. Samplers were




positioned at a height of 1.5 m on public land such as rights-of-way. Sam-




plers were located at 0 and 1/8 mile from the plant perimeter. Locations




of the five stations are shown in Figure 4. Site descriptions and air sam-




pling data are given in Table 12.




Water Sampling - Four water samples were taken; two grab samples and two




composite samples. The first composite sample, W-l, was taken over a 14-




hr period from 1900 August 9, 1977, to 0900 August 10, 1977, from the




bridge where Highway 156 passes over Bailey Creek upstream of the plant




and municipal sewage treatment facility. The second composite sample, W-4,




was taken over a 14-hr period from 2400 August 9, 1977, to 1400 August 10,




1977, from the bridge where Highway 10 passes over Bailey Creek downstream




of the plant. Grab samples were taken at locations W-2 and W-3. The water




sampling locations are shown in Figure 4 and described more thoroughly




in Table 13.





Soil Sampling - Four soil samples were taken on August 10, 1977, one from




each of the air sampling sites (see Table 12). Soil samples were taken by




removing approximately the top 12 ram of soil from the area close to the





air sampler.
                                   35

-------
                         Table 12.   AIR SAMPLING DATA FOR HERCULES. INC., HOPEWELL. VIRGINIA



Area
Northwest transect,
0 km

North transect,
0 km
North transect,
0 km
South transect,
0.3 km

Northeast transect,
0 km


Sample^'
No.
A-l
(So-1)

A- 2
(So-2)
A- 3
(So-3)
A-4
(So-4)

A- 5
(So-5)





Total Sampling
sampling rate
Exact location, USGS coordinates (hr) (£/min)
0 km north-
west at plant
gate
0 km north
on plant road
0 km north
on plant road
0.3 km south-
west at Hopewell
sewage plant
0 km east at
northeast corner
of plant
4129450 m
297560 m

4129320 m
298150 m
4129320 m
298150 m
4128120 m
297310 m

4128880 m
298830 m

N 25.3 0.52
E

N 26.9 0.29
E
N 26.9 0.40
E
N 25.1 0.18
E

N 25.1 0.62
E

Total Sample
volume height
(m3) (m)
0.79 1.5


0.46 1.5

0.65 1.5

0.28 1.5


0.93 1.5


jj/  Soil samples were collected at  these air sampling stations.

-------
     Table 13.   WATER AND SEDIMENT SAMPLING DATA FOR HERCULES,  INC.,
    	HOPEWELL. VIRGINIA	
Sample
 No.

 W-l
 W-2
 W-3
 W-4
 Se-1
Exact location, USGS coordinates
Highway 156 at
Bailey Creek

Bailey Creek below
Outfall 002
4127320 m N
 296950 m E

4128140 m N
 297330 m E
Bailey Creek below   4128050 m N
Outfall 003           298240 m E
Highway 10 at
Bailey Creek

Bailey Creek below
Outfall 004
4129050 m N
 297810 m E

4128430 m N
 298380 m E
     Sample
      type

Composited water
Grab water
               Grab water
 Volume
collected

1,000 ml


1,000 ml


1,000 ml
Composited water    1,000 ml
Sediment
1,000 cm3
                                    37

-------
Sediment Sampling - A sediment sample was taken from below an outfall be-


lieved to be No. 004 which, according to the NPDES permit application,


should contain process waste from noncellulose polymer production.  The


sample was taken by scraping up some of the clay soil beneath the water


level. The sampling data are given in Table 13.


Meteorological Conditions


     The wind conditions existing at Byrd Airport, Richmond,  Virginia,


between 0800 August 9, 1977, and 1300 August 10, 1977, are summarized in


Table 14. Locally winds blew predominantly from the southwest.


Sample Analysis


Air Samples - The results of the analysis of the five air samples and two


blanks are listed in Table 15. No AA was found in the Chromosorb  101  ex-


tracts or in the filter extracts. The minimum detectable concentration,


which was dependent upon the volume of air collected, ranged from 0.3 to

      3
1 |J.g/m .  The absence of AA in air sample No. 3 was later confirmed by


GC/MS (vide infra).


Water Samples - The results of the analysis of the water samples  are


given in Table"16. No AA was found in Samples W-l, W-2, or W-3. The limit


of detection for these samples, taking into account the 500-fold  increase


in concentration by evaporation,  was approximately 0.8 (jtg/liter.  Sample


4, however, was found to contain 1.5 ppm AA. This was the only sample


taken downstream of outfall No. 004. The presence of AA in the sample was


confirmed by GC/MS (vide infra).
                                   38

-------
Table 14.  WEATHER CONDITIONS DURING SAMPLING AT HERCULES,  INC,
             HOPEWELL, VIRGINIA:  SOURCE OF DATA,
             BYRD AIRPORT* RICHMOND, VIRGINIA

Wind
Time (EOT)
August 9. 1977
0800
0900
1000
1100
1 200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
August 10, 1977
0100
0200
0300
0400
0500
0600
0700
0800
0900
1000
1200
1300
Direction

W
NW
N
NE
NNW
N
NE
E
S
SW
S
E
ENE
E
E
SE
SE

ESE
E
SE
SE
SSW
S
S
SW
SW
SW
WSW
W
Speed (knots)

5
3
3
3
6
8
5
4
5
3
3
5
5
6
7
5
5

4
4
4
4
3
3
3
5
5
6
9
9
Precipitation

None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None

None
None
None
None
None
None
None
None
None
None
None
None
                                 39

-------
        Table 15.  ACEYLAMIDE CONCENTRATIONS IN AIR SAMPLES FROM
                   HERCULES, INC., HOPEWELL. VIRGINIA

Sample
No.
A-l
A- 2
A- 3
A-4
Blank 1

Blank 2

Sampling
8/9/77
8/10/77
8/9/77
8/10/77
8/9/77
8/10/77
8/9/77
8/10/77
_

_

period
1049-
1207
0924-
1219
0924-
1219
1035-
1144




Volume Type of
(cu m) sample
0.79 Aic§/
Particulate^/
0.46 Air
Particulate
0.65 Air
Particulate
0.28 Air
Particulate
Air
Particulate
Air
Particulate
yg
found
< 0.3
< 0.8
< 0.3
< 0.8
< 0.3
< 0.8
< 0.3
< 0.8
< 0.3
< 0.8
< 0.3
< 0.8
Ug/m3
< 0.4£/
< 1.1
< 0.6
< 1.6
< 0.5
< 1.3
< 1.1
< 0.8
_
-
_

_a/  Chromosorb 101 extracts.
_b/  Millipore filter extracts.
_c/  Based on instrumental detection  limit and volume of air sampled.
      Table  16.  ACRYLAMIDE CONCENTRATIONS IN WATER  SAMPLES FROM
                  HERCULES, INC., HOPEWELL, VIRGINIA


Sample
No.
W-l
W-2
W-3
W-4
Aliquot
volume
(ml)
250
250
250
250
Final
volume
(ml)
0.5
0.5
0.5
8.3

yg
found
< 0.2
< 0.2
< 0.2
383


ys/£
< o.as/
<0.8
< 0.8
1,500
_a/  Based upon instrument detection  limit and  sample  size.
                                    40

-------
Soil and Sediment Samples - The results of the analysis of the soil  and

sediment samples are listed in Table 17. No AA was found in any of the  sam-

ples. The limit of detection was approximately 0.08 |j,g/g.
   Table  17.  ACBYLAMIDE CONCENTRATIONS IN SOIL AND SEDIMENT SAMPLES
                 FROM HERCULES, INC., HOPEWELL, VIRGINIA


Sample
No.
So-1
So-2
So- 4
So- 5
Se-1
Sample size
wet
(a)
50
50
50
50
50
Final
volume
(ml)
5
5
5
5
5

yg
found
< 4
< 4
< 4
< 4
< 4


US/R

-------
   100-


    90-




    70-j


    60-1




    40-j


    304


    £04
'I
20
                                                            - 30
                                                            r £0
        0       10     20     30      40     50

SPECO  15490  Jtl  fiCRYLfltUDE  30C    J. 6.
           "I""!""1""!""1""!   "
 60     70      80     90    100

•£S-?7                STEP  hfl3S=l/ ixBxS
         Figure 5.  Mass spectrum of acrylamide

-------
     Data system:  Varian Spectrosystem 100 MS




     Separator:  Watson-Biemann




     Mass monitored:  71.0371




     Resolution:   7,000




     lonization potential:  70 ev




     Emission current:  1 mA




     Multiplier gain:  4 x 106 - 2 x 107




     The resolution chosen, 7,000, was that necessary to  provide  baseline




resolution of the AA 71 peak from the background 71 peak.




     The presence of AA in water sample No. W-4 was first confirmed  as




shown by the ion current plots in Figure 6. The raw sample rather than




the concentrated sample was analyzed. No attempt was made to  quantitate




the AA in W-4 by GC/MS.




     The absence of AA in the concentrated air sample No. A-3 was also




confirmed by this technique. The ion current plot is also shown in Fig-




ure 6 with the expected elution time of AA noted. The sensitivity of the




GC/MS system in the high resolution mode was comparable to that of the




GC/Hall detector. Thus, the limit of detection was about  0.5  M«g/m3 for




air sample No. A-3.




CALGON CORPORATION, ELLWOOD CITY, PENNSYLVANIA




Field Sampling




     A presampling survey was conducted on July 25, 1977. The Calgon plant




lies in Frisco Township of Beaver County, Pennsylvania, just  east-southeast




of Ellwood City, Pennsylvania. The plant is located in a semi-rural  area






                                    43

-------
   X 188
RE40Y
            MU- -2.2KU
           MOng
           Acrylomide
                                                              W-4
                                                              !"!1
         s          10          is         20
                     MINUTES
97/  MI/   HERCULES W 4  RAW   J GOING 6  23  77
                 a*   Water  sample W-4
                                                          23
RE>
    X 188
   DY

          -r- J   •  I   |  i  I   |  I   I  |—i  I  |   I  I   |  r
      1       2       34      S       6       7
                     MINUTES
98/ MI/   HERCULES A IR  3 CONC J  GOING  6 25  77
                 b.   Air sample A-3
 Figure 6.  Selection ion current plots,  m/e = 71.0371
                                                                e
                             44

-------
near a bend in the Connoquenessing Creek, which lies to the north and west




of the plant. The east side of the plant lies near an abandoned roadbed.




There is a small chemical company adjacent to the plant on the south side




which reportedly supplies Calgon with chlorine for water treatment.




     The NPDES permit for this plant indicates that there are four dis-




charge points to an unnamed tributary of Connoquenessing Creek. The trib-




utary was found 200 yards east of a railroad trestle over River Road. This




point is marked W-2 on the map shown in Figure 7. It was assumed that pro-




cess water came from the local supply as no other source was located. This




Calgon facility manufactures slowly soluble phosphates, water soluble poly-




mers, and water treatment compounds.




     Field sampling was carried out from 0800 (EOT) August 30, 1977, to




1300 (EOT) August 31, 1977. Four air samplers were deployed at four sites




upwind, downwind, and laterally with respect to the plant. Soil samples




were taken from each air sampling site; water samples were taken at vari-




ous locations upstream and downstream from the points of discharge; sedi-




ment samples were taken below two discharge points.




Air Sampling - Air sampling was carried out from 0800 (EDT) August 30,




1977, to 1100 (EDT) August 31, 1977, using four samplers at four sites.




The sites were located upwind, downwind, and laterally with respect to




the plant. Samplers were positioned at a height of 1.5 m from the ground




on public land such as rights-of-way. Locations of the samplers are shown




in Figure 7. Site descriptions and air sampling data are given in Table





18.






                                   45

-------


                    * f.*\.       V.
                    ••.•at  =i.,».  \
                                                     Air & Soil Sampling Site



                                                W^) Water Sampling Site



                                               SE  ^ Sediment Sampling Site
Figure 7.   Sampling locations at  Calgon Corporation,

               Ellwood City,  Pennsylvania
                         46

-------
               Table 18.   AIR SAMPLING  DATA FOR CALGON  CORPORATION.  ELLHDOD  CITY,  PENNSYLVANIA

Area
North transect,
0 km
South transect,
0 km
East transect,
0 km
West transect,
0 km
Sample^/
No.
A-l
(So-1)
A- 2
(So- 2)
A- 3
(So-3)
A- 4
(So-4)
Exact location, USGS
0 km north on
River Road
0 km south on
Main Street
0 km east on
Forest Grove Avenue
0 km west on
Hazen Avenue
coordinates
4522420 m N
561320 m E
4522280 m N
561210 m E
4522360 m N
561330 m E
4522160 m N
560920 m E
Total
sampling
(hr)
25.6
25.5
25.5
25.5
Sampling
rate
(Jt/min)
0.80
0.63
0.58
0.61
Total
volume
(m3)
1.22
0.97
0.89
0.95
Sample
height
(m)
1.5
1.5
1.5
1.5
jj/  Soil,  samples were  collected  at  these  air  sampling  stations.

-------
Water Sampling - Five water samples were taken at various points  upstream




and downstream of the discharge points. All were composite samples  taken




during the period 0800 (EOT) August 30, 1977,  to 1300 (EOT) August  31,




1977. Although the NPDES permit describes four discharge points  into  an




unnamed tributary of the Connoquenessing Creek, only two outfalls were ob-




served from the edge of the creek. One, W-4, is known to come from  the




plant and the other, W-3, may come from the plant. Both outfalls  were




large diameter concrete pipes which passed under River Road and  flowed




down the rocky bank of the Connoquenessing. Samples W-3 and W-4 were




taken directly from outfalls; the remaining samples were taken from




Connoquenessing Creek. Sampling locations are shown in Figure 7  and de-




scribed in Table 19.




Soil Sampling - Soil samples were taken on August 31, 1977, at each of




the air sampling sites (see Table 18) by scraping up the top 10 mm  of




soil.




Sediment Sampling - Sediment samples Se-3 and Se-4 were taken with  water




samples W-3 and W-4, which are at plant outfalls. Samples were taken  by




scraping some sediment up into jars. Sampling data are given in  Table 19.




Meteorological Conditions




     The wind conditions existing at Pittsburgh International Airport be-




tween 0800 (EOT) August 30, 1977, and 1100 (EDT) August 31, 1977, are sum-




marized in Table 20. Locally the winds appeared calm during the  period.
                                   48

-------
         Table  19.   WATER AND SEDIMENT SAMPLING DATA FOR CALGON CORPORATION,  ELLWOOD CITY,  PENNSYLVANIA
v£>
Sample
No.
W-l
W-2
W-3
Se-3
W-4
Se-4
W-5
Exact location, USGS
0.5 km upstream
of Outfall 001
West side of
railroad trestle
East side of rail-
road trestle, di-
rectly from Out-
fall 001
0.3 km east of
railroad trestle,
directly from Out-
fall 002
0.6 km east of
railroad trestle
coordinates
4521950 m N
560930 m E
4522390 m N
560950 m E
4522380 m N
561010 m E
4522480 m N
561300 m E
4522750 m N
561720 m E
Sample type
Composite water
Composite water
Composite water
Sediment
Composite water
Sediment
Composite water
Volume
collected
1,000 ml
1,000 ml
1,000 ml
1,000 cm3
1,000 ml
1,000 cm3
1,000 ml

-------
Table 20.  WEATHER CONDITIONS DURING SAMPLING AT GALGON CORPORATION,
             ELLWOOD CITY, PENNSYLVANIA:  SOURCE OF DATA,  PITTSBURGH
             INTERNATIONAL AIRPORT, PITTSBURGH. PENNSYLVANIA

Wind
Time (EOT)
Aueust 30, 1977
0800
0900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
August 31, 1977
0100
0200
0300
0400
0500
0600
0700
0800
0900
1000
1100
Direction

WNW
N
WSW
NE
N
NNE
N
-
NW
N
N
E
SE
E
-
-
S

—
-
-
-
-
-
_
SE
_
SE
S
Speed (knots)

3
5
3
4
4
4
4
0
3
6
5
4
3
3
0
0
3

0
0
0
0
0
0
0
3
0
3
3
Precipitation

None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None

None
None
None
None
None
None
None
None
None
None
None
                                   50

-------
Sample Analysis




Air Samples - The results of the analysis of the four air samples are




listed in Table 21. No AA was found in the Chromosorb 101 extracts or fil-




ter extracts. The limits of detection, which are dependent upon the vol-




ume of air actually sampled, were about 0.1 p,g/m^ for air and 0.4 (j,g/m-^




for particulates. No AA was found in the field blanks.




Water Samples - The results of the analysis of the five water samples are




listed in Table 22. No AA was found in any of the samples after evapora-




tion of 250 ml to 1 ml. The limit of detection was 0.8 (jtg/liter.




Soil and Sediment Samples - The results of the analysis of the soil and




sediment samples are summarized in Table 23. No AA was found above the




detection limit of 0.02 |J
-------
            Table 21.  AGRYLAMIDE  GONCENTRA.TIONS  IN AIR SAMPLES  FROM GALGON CORPORATION,
                                      ELLWOOD  CITY. PENNSYLVANIA

Sample No.
A-l
A-2
A-3
A-4
Sampling
(hr)
8/30/77
8/31/77
8/30/77
8/31/77
8/30/77
8/31/77
8/30/77
8/31/77
period
0845-
1030
0823-
0945
0843-
1014
0909-
1040
Volume
(m3)
1.22
0.97
0.89
0.95
Type of
sample
Air*/
Particulate—'
Air3/
Particulate^'
Particulatek/
Air^/
Particulate—
M-8
found
< 0.1
< 0.4
< 0.1
< 0.4
< 0.1
• < 0.4
< 0.1
< 0.4
/m3
< O.l£/
< 0.4£/
< 0.1
< 0.4
< 0.1
< 0.4
< 0.1
< 0.4
al  Chromosorb 101 extracts.
b_/  Millipore filter extracts.
c_/  Based upon instrument  detection  limit  and  sample  size.

-------
      Table 22.  AGRYLAMIDE CONCENTRATIONS IN WATER SAMPLES FROM
            CALGON CORPORATION. ELLWOOD CITY, PENNSYLVANIA



Sample No.
W-l
W-2
W-3
W-4
W-5
Aliquot
volume
(ml)
250
250
250
250
250
Final
volume
(ml)
1
1
1
1
1

M-g
found
< 0.2
< 0.2
< 0.2
< 0.2
< 0.2

/
UB/lS/
< 0.8
< 0.8
< 0.8
< 0.8
< 0.8
a/  Based upon instrumental detection limit and sample size.
  Table 23.   ACRYLAMIDE  CONCENTRATIONS  IN  SOIL AND  SEDIMENT  SAMPLES
         FROM CALGON CORPORATION,  ELLWOOD  CITY.  PENNSYLVANIA


Sample
No.
So-1
So- 2
So- 3
So-4
Se-1
Se-2
Sample size
wet
(e)
50
50
50
50
50
50
Final
vo lume
(ml)
5
5
5
5
0.5
0.5

yg
found
< 1
< 1
< 1
< 1
< 1
< 1


ys/s
< 0.022/
< 0.02J/
< 0.02J/
< 0.022/
< 0.02-2/
< 0.022/
_a/   Based upon instrument detection limit and wet sample  size.
                                  53

-------
                                           A-l )    Air & Soil Site



                                           001 !-»•  Discharge Point



                                           W-1 )    Water Site
              K» a     :
              \/i :!    IS'
Figure  8.  Sampling locations at Nalco Chemical  Company, Garyville,  Louisiana
                                      54

-------
     Field sampling was conducted during the period 0900 September 13,




1977, to 1900 September 14, 1977» Four air samples and three water samples




were collected at that time. Water samples were collected later.




Air Sampling - Air sampling was conducted using four samplers at  four sam-




pling sites which were positioned upwind, downwind, and laterally with re-




spect to the plant. Samplers were located at 0 and 1/8 mile from  the plant




property and were positioned at a height of 1.5 m on adjacent property,




a railroad right-of-way, and a levee.




     Sampling was carried out during the period 0930 (GDI) September 13,




1977, to 1830 (GDI) September 14, 1977, and was often interrupted by rain.




The four sampling locations are shown in Figure 8. Site descriptions and




air sampling data are given in Table 24.




Water and Sediment Sampling - Due to the extreme difficulty in obtaining




a boat and the inclement weather, no water or sediment samples were taken.




Arrangements were then made with the Jefferson Parish Sheriff's Office to




have one of their personnel collect water samples; these samples  were re-




ceived and analyzed. This office was involved in the collecting of a water




sample from the American Cyanamid site at Fortier. For this reason, it is




felt that samples of acceptable quality were collected. Sampling  data are




given in Table 25.




Soil Samples - Soil samples were taken September 14, 1977, at three of the




four air sampling sites by removing the top approximately 10 mm of topsoil




from the area by each sampler. Sampling data are given in Table 26.
                                   55

-------
Table 24..  AIR SAMPLING DATA FOR NALCO CHEMICAL COMPANY,  GARYVILLE.  LOUISIANA

Area
JJorth transect,
0 km

South transect,
0.2 km
Southeast transect,
0 km
West transect,
0 km
Samp le
No. Exact location, USGS
A-l Plant spur and main
line Illinois Cen-
tral Railroad
A-2 0.2 km south on
levee
A- 3 0 km east along
State Highway 44
A-4 0 km west along
fenceline
coordinates
3327400 m N
728720 m E

3325960 m N
728300 m E
3325810 m N
728710 m E
3326320 m N
728160 m E
Total
sampling
(hr)
24

23.1
10.6
23.5
Sampling
rate
(Jl/min)
0.65

0.49
0.63
0.62
Total
volume
(m3)
0.93

0.68
0.40
0.88
Sample
height
(m)
1.5

1.5
1.5
1.5

-------
      Table 25.   WATER SAMPLING DATA FOR NALCO CHEMICAL COMPANY
                         GARYVILLE. LOUISIANA	
Sample
 No.

 W-l
 W-2
Exact location, USGS coordinates
30 m upstream of
Discharge 001,
20 m offshore

At Discharge 001
3325820 m N
 728270 m E
3325770 m N
 728290 m E
                                Vo lume
                Sample type    collected
 Grab
 Grab
1,000 ml
1,000 ml
 W-3      150 m downstream
          of Discharge 001,
          20 m offshore
                    3325650 m N
                     728390 m E
                    Grab
            1,000 ml
      Table 26.   SOIL SAMPLING DATA FOR NALCO CHEMICAL COMPANY
     	GARYVILLE. LOUISIANA	
Sample
 No.

 So-1
 So-2
 So-4
Exact location, USGS coordinates
                                 Percent
                 Sample size    moisture
Plant spur and main
line Illinois Cen-
tral Railroad

0.2 km south on
levee

0 km west along
fenceline
  3327400 m N
   728720 m E
  3325960 m N
   728300 m E

  3326320 m N
   728160 m E
250 cm3
250 cm3
250 cm3
    89
    64
    75
                                   57

-------
Meteorological Conditions - The meteorological conditions that existed dur-




ing the sampling period were obtained from the National Weather Service,




New Orleans International Airport, and are summarized in Table 27.




Sample Analysis




Air Samples - The results of the analysis of the four air samples and the




blank are listed in Table 28. No AA was found in the Chromosorb 101 ex-




tracts or filter extracts. The limits of detection were about 0.15  pg/m?




for air and 2 Mig/m  for particulates* This level was established using




spiked samples* No AA was found in the field blanks*




Water Samples - The results of the analysis of the three water samples are




listed in Table 29. No AA was found above a limit of detection of about




0.8 |4/g/liter, established with spiked samples.




Soil Samples - The results of the analysis of the three soil samples are




summarized in Table 30. No AA was present above the detection limit of




0.02 (Jig/g» established with spiked samples.




AMERICAN CYANAMID, FORTIER, LOUISIANA




Field Sampling




     A presampling survey of the American Cyanamid Company plant, Fortier,




Louisiana, was conducted on September 14, 1977. The plant is located in




Jefferson Parish, Louisiana, in South Kenner along State Highway 18 about




6 miles east of Luling, Louisiana. The plant lies in a semi-rural area




with the Mississippi River to the north. The NPDES permit shows two out-




falls which discharge wastewater into the Mississippi River. Both are
                                   58

-------
     Table 27.  WEATHER CONDITIONS DURING SAMPLING AT NALCO CHEMICAL,
            GARYVILLE, LOUISIANA:  SOURCE OF DATA, NEW ORLEANS
              INTERNATIONAL AIRPORT, NEW ORLEANS, LOUISIANA


Time (GOT)
September 13. 1977
0900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
September 14, 1977
0100
0200
0300
0400
0500
0600
0700
0800
0900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900

Direction

S
S
S
S
S
SSW
S
S
0
NNE
0
NW
E
E
ESE
ESE

ESE
SSE
S
S
SSW
NW
W
E
SSW
SSE
S
S
SW
S
S
S
S
SSE
S
Wind
Speed (knots)

6
9
9
3
10
11
11
8
0
3
0
4
3
4
4
2

5
4
7
5
8
12
8
2
7
11
10
6
7
10
9
7
6
7
5

Precipitation^/

None
None
None
None
None
None
None
Rain
Rain
Rain
None
None
None
None
None
None

None
Rain
None
None
None
Rain
None
None
Rain
None
None
None
None
None
None
None
None
None
None
a]  Precipitation data from sampling site.

                                     59

-------
           Table 28.  AGRYLAMIDE CONCENTRATIONS IN AIR SAMPLES FROM NALCO CHEMICAL, GARYVILLE. LOUISIANA
a*
o
Sample No.
A-l
A-2
A-3
A-4
Sampling period
(hr)
9/13/77
9/14/77
9/13/77
9/14/77
9/13/77
9/14/77
9/13/77
9/14/77
0930-
1714
1010-
1806
1037-
1045
1111-
1740
Volume
(m3)
0.93
0.68
0.40
0.88
Type of
sample
Air*/
Particulatek'
Air3/
Particulatek/
Particulate^
Air*/
Part icu latell/
M-S
found
< 0.1
< 0.4
< 0.1
< 3.2
< 0.1
< 1.6
< 0.1
< 1.6
ue/m
< O.llS/
< 0.43£/
< 0.15
< 4.7
< 0.25
< 4.0
< 0.12
< 1.8
       a/  Ghromosorb  101 extract.
       b/  Millipore filter  extract.
       c_/  Based upon  instrument  detection  limit and sample size.

-------
     Table 29.  ACRYLAMIDE CONCENTRATIONS IN WATER SAMPLES FROM
            NALCO CHEMICAL COMPANY. GARYVILLE. LOUISIANA


Sample
No.
W-l
W-2
W-3
Aliquot
volume
(ml)
250
250
250
Final
volume
(ml)
1
1
1

yg
found
< 0.2
< 0.2
< 0.2


vz/a
< o.ss/
< 0.8
< 0.8
_a/  Based upon instrument detection limit and sample size,
        Table  30.  ACRYLAMIDE CONCENTRATIONS IN SOIL SAMPLES
            NALCO CHEMICAL COMPANY. GARYVILLE. LOUISIANA


Sample
No.
So-1
So- 2
So- 3
Sample size
wet
(a)
50
50
50
Final
volume
(ml)
1
1
1

yg
found
< 1
< 1
< 1


us/g
< 0.02J/
< 0.02
< 0.02
_a/  Based upon  instrumental detection  limit and wet sample size.
                                  61

-------
readily visible passing over the highway. The locations are shown in Fig-




ure 9. This plant produces AA and polyacrylamide through catalytic conver-




sion of acrylonitrile»




     Field sampling was conducted during the period 2000 September 14,




1977, to 0700 September 16, 1977. Four air samples, three water samples,




four soil samples, and two sediment samples were collected.




Air Sampling - Air sampling was carried out from 2000 (CDT) September 14,




1977, to 0700 (CDT) September 16, 1977, at four sampling sites located




upwind, downwind, and laterally with respect to the plant. Samplers were




located at 1/8 and 3/4 mile from the plant perimeter and were positioned




at a height of 1.5 m on street rights-of-way, a railroad right-of-way,




and on a levee. Sampling was interrupted by rain. The four sampling loca-




tions are shown in Figure 9. Site descriptions and air sampling data are




given in Table 31.




Water and Sediment Sampling - Water and sediment samples were collected




on September 16, 1977, from upstream, downstream, and between the two dis-




charge points shown in Figure 9. Sampling data are given in Table 32.




Soil Sampling - Soil samples were taken on September 16, 1977, at each  of




the four air sampling sites by removing approximately the top 10 mm of




soil from the area near each sampler. Sampling data are given in Table  33.




Meteorological Conditions - The meteorological conditions that existed




during the sampling period were obtained from the National Weather Service,




New Orleans International Airport, and are summarized in Table 34.
                                   62

-------
                            Air i Soil Site




                            Water Site




                            Sediment Site
Figure  9,   Sampling  locations  at  American  Cyanamid, Fortier, Louisiana
                                        63

-------
Table 31.  AIR SAMPLING DATA FOR AMERICAN CYANAMID. NEW ORLEANS. LOUISIANA

North
0.2
South
1.2


East
0.4
West
0.2
Area
transect,
km
transect,
km


transect,
km
transect,
km
Samp le
No. Exact location, USGS
A-l 0.2 km north on
levee
A- 2 1.2 km south on
Texas and Pacific
Railroad right-of-
way
A-3 0.4 km east on
Moss Lane
A-4 0.2 km west on
Fortier Heights
coordinates
3317850
763350
3315260
762950


3317650
764380
3317220
762870
m
m
m
m


m
m
m
m
N
E
N
E


N
E
N
E
Total
sampling
(hr)
23.3
24.2


23.0
23.2
Sampling Total
rate volume
(£/min) (m3)
0.64 0.89
0.69 1.01


0.69 0.95
0.52 0.72
Sampling
height
(m)
1.5
1.5


1.5
1.5
             Road

-------
Table 32.  WATER AND SEDIMENT SAMPLING DATA FOR AMERICAN CYANAMID,  NEW ORLEANS,  LOUISIANA
Sample
No.
W-.l
W-2
W-3
Se-1
Se-2

Exact location, USGS
50 m upstream of
Discharge 001
Midway between
Discharge Points
001 and 002
50 m downstream of
Discharge 002
50 m upstream of
Discharge 001
50 m downstream of
Discharge 002
coordinates Sample type
3317850 m N Grab water
762940 m E
3317910 m N Grab water
763330 m E
3317970 m N Grab water
763560 m E
3317850 m N Sediment
762940 m E
3317970 m N Sediment
763560 m E
Volume
collected
1,000 ml
1,000 ml
1,000 ml
500 cm3
500 cm3

Percent moisture
by weight
-
••
-
47
64


-------
         Table 33.  SOIL SAMPLING DATA FOR AMERICAN CYANAMID
         ....	NEW ORLEANS, LOUISIANA	
Sample
 No.

 So-1
 So-2
 So-3
 So-4
Exact location, USGS coordinates
0.2 km north on
levee

1.2 km south on
Texas and Pacific
Railroad right-of-
way

0.4 km east on Moss
Lane
3317850 m N
 763350 m N

3315260 m N
 762950 m E
3317650 m N
 764380 m E
0.2 km west on        3317220 m N
Fortier Heights Road   762870 m E
                               Percent
                Sample size    moisture
250 cm3
250
250 cm3
                  250 cm-
78
78
70
                67
                                  66

-------
    Table 34.   WEATHER CONDITIONS DURING  SAMPLING AT AMERICAN CYANAMID,
            NEW ORLEANS,  LOUISIANA:   SOURCE OF DATA, NEW ORLEANS
               INTERNATIONAL AIRPORT. NEW ORLEANS, LOUISIANA


Time (GOT)
September 14, 1977
2000
2100
2200
2300
2400
September 15, 1977
0100
0200
0300
0400
0500
0600
0700
0800
0900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300
2400
September 16, 1977
0100
0200
0300
0400
0500
0600
0700

Direction

SW
0
0
S
0

0
0
0
0
0
0
0
NE
0
NNW
W
0
WNW
W
W
ENE
E
N
ENE
E
E
ESE
0
E

0
ESE
E
E
0
ESE
ESE
Wind
Speed (knots)

5
0
0
4
0

0
0
0
0
0
0
0
3
0
3
13
0
6
5
3
4
6
2
6
6
9
3
0
2

0
3
2
4
0
2
2

Precipitation^/

None
None
None
None
None

None
None
None
None
None
None
Rain
Rain
Rain
Rain
Rain
Rain
Rain
Rain
Rain
Rain
Rain
None
None
None
None
None
None
None

None
None
None
None
None
None
None

a/  Precipitation data from sampling site.
                                    67

-------
Sample Analysis


Air Samples - The results of the analysis of the four air samples  and the


blank are listed in Table 35. No AA was found in the Chromosorb  101  ex-


tracts or filter extracts. The limits of detection of about  0.12 |ig/nr for

                o'
air and 0.8 |ig/m  for particulates were established using spiked samples.


No AA was found in the field blanks.


Water Samples - The results of the analysis of the three water samples are


given in Table 36. No AA was found above the detection limit of  0.8  ^g/liter


established using spiked samples.


Soil and Sediment Samples - The results of the analysis of the six soil


and sediment samples are summarized in Table 37. No AA was present above


0.02 M>8/8 as established using spiked samples.


ST. REGIS PAPER COMPANY, SARTELL, MINNESOTA


Field Sampling


     A presampling survey of the St. Regis Paper Company plant in Sartell,


Minnesota, was conducted on October 6, 1977. The plant is located in


Benton County, Minnesota, just east of the corporate limits of Sartell,


Minnesota. The plant is bounded on the west by the Mississippi River and


on the east by State Highway 152. The DeZurik Company plant is located


on the opposite side of the Mississippi River. Wastewater is discharged


into the Mississippi River at the points shown in Figure 10. Only water


and sediment samples were scheduled to be taken at this site.
                                   68

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    Table 35.  ACRYLAMIDE CONCENTRATIONS IN AIR SAMPLES FROM AMERICAN CYANAMID.  FORTIER.  LOUISIANA

Sample No.
A-l

A-2

A-3

A-4

Sampling
period Volume
(hr) (m3)
9/14/77
9/16/77
9/14/77
9/16/77
9/14/77
9/16/77
9/14/77
9/16/77
2025- 0.89
0538
2145- 1.01
0658
2100- 0.95
0625
2045- 0.72
0609
Type
of
sample
Air*/

Particulate— '
Alra/

ParticulateV
AlrS/

ParticulatelJ/
Aira/

Particulate^.'

U>
g
found
<
<
<
<
<
<
<
<
0
0
0
0
0
0
0
0
.1
.4
.1
.8
.1
.8
.1
.8



y.g/m3
<
<
<
<
<
<
<
<
0
0
0
0
0
0
0
1
,12£/
.45£/
.10
.80
.11
.85
.14
.12
a/  Ghromosorb 101 extract.
b/  Millipore filter extract.
c_/  Based on instrumental detection limit and volume of air sampled.

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    Table  36.  ACRYLAMIDE CONCENTRATIONS IN WATER SAMPLES FROM
              AMERICAN CYANAMID. FORTIER. LOUISIANA


Sample
No.
W-l
W-2
W-3
Aliquot
volume
(ml)
250
250
250
Final
volume
(ml)
1.0
1.0
1.0

yg
found
< 0.2
< 0.2
< 0.2


us /«.
< 0.&2/
< 0.8
< 0.8
_a/  Based upon instrument detection  limit and  sample size.
  Table 37.   ACRYLAMIDE CONCENTRATIONS IN SOIL AND SEDIMENT SAMPLES
             FROM AMERICAN CYANAMID*  FORTIER,  LOUISIANA


Sample
No.
So-1
So- 2
So-3
So-4
Se-1
Se-2 ,
Sample size
wet
(g)
50
50
50
50
50
50
Final
volume
(ml)
1
1
1
1
1
1

yg
found
< 1
< 1
< 1
< 1
< 1
< 1


yg/g
< 0.022/
< 0.02
< 0.02
< 0.02
< 0.02
< 0.02
_a/   Based  upon instrumental  detection limit and wet sample  size.
                                  70

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     Due to the rocky nature of the river bottom,  sediment  samples  were




not obtained. Grab water samples were taken on October 6,  1977,  from two




locations, upstream and downstream from the discharge points,  as shown in




Figure 10. Water sampling data are given in Table  38.




Sample Analysis




     The results of the analysis of the two water  samples  are  given in




Table 39. No AA was found above the detection limit of 0.8 jig/liter estab-




lished using spiked samples.




DOW CHEMICAL, MIDLAND, MICHIGAN




     The environmental samples from the Dow plant  were collected and ana-




lyzed by another contractor,—  using in some instances, different metho-




dologies. Some of the results are included in this report  so  that all of




the environmental measurements will be available in a single  document.




     Grab 1,000 ml water samples were collected from the points  indicated




in Figure 11. The results of the analyses are given in Table  40. The re-




ported detection limit was 25 ppb.




     Air samples were collected at the six stations shown  in  Figure 11.




The results of the analysis for both vapor and particulate AA  are given




in Table 41. No AA was reported above the 1 p-g/nH  detection limit.




     Soil samples, collected at the six air sampling stations, were ana-




lyzed with the results given in Table 42. No AA was reported  above  20 ppb.
                                   71

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       UNITED STATES
DEPARTMENT OF THE INTERIOR
     GEOLOGICAL SURVEY
       •03«lo-£.        1 94000O FCET (
                                                                             STATE OF MINNESOTA
                                                                          DEPARTMENT OF ADMINISTRA
                                                                                    Tin „ HW   ,11 •• .'
                                                                               "07 ILITTLC &<>'.* I AXEI  •*>''.

                                                 Water Site

                                              •* Discharge Point
S500CO
   'SOUTH
             Figure 10.   Sampling  locations  at St.  Regis Paper Company,
                             Sartell,  Minnesota
                                            72

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    Table 38.  WATER SAMPLING DATA FOR ST. REGIS PAPER COMPANY,
                        S ARTELL.MINNE SOTA

Sample
No.
W-l
W-2
Exact location, USGS
0.6 km north on
152 from bridge
State Highway 152
bridge over
coordinates
5052750 m N
406230 m E
5052150 m N
406270 m E
Sample type
Grab water
Grab water
Volume
collected
1,000 ml .
1,000 ml
         Mississippi River
 Table 39.  ACRYLAMIDE CONCENTRATIONS IN WATER SAMPLES FROM ST. REGIS
                  PAPER COMPANY, SARTELL. MINNESOTA


Sampling
station
W-l
W-2
Aliquot
volume
(ml)
250
250
Final
volume
(ml)
1
1

yg
found
< 0.2
< 0.2


U2/£
< 0.8S/
< 0.8
_a/  Based upon instrumental detection limit and sample size.
                                   73

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                                                    #SITE NUMBER
                                                        HIGHWAY
                            I ! I I I I I I I I I I I I I I I I I I Kl
                                                       RAILROAD
                                                       PLANT SITE
                                                       RESIDENTIAL
                                                           AREA
                                                      SOIL/AIR SAMPLE
             KILOMETER

          0     .5      1
                                                    A  WATER SAMPLE
Figure 11.  Map of sampling sites for acrylamide at Midland, Michigan
                                   74

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 Table 40.  ANALYTICAL DATA. FOR WATER SAMPLES FROM MIDLAND. MICHIGAN!/
Sampling                                                      Acrylamide
station                                                        analysis
 letter            Exact location. USGS coordinates            (ppb)—'

   A          Tittabawassee River, upstream   4832000 m N        < 25
              of chemical plant                722442 m E

   B          Tittabawassee River, down-      4827500 m N        < 25
              stream of chemical plant         726231 m E

   C          Effluent from outfall at        4829692 m N        25-125
              Lingle Drain near Dow            725308 m E
              Corning

   D          Tap water samples from                             < 25
              Holiday Inn

   E          Snow sample taken at air        4832910 m N        < 25
              sampling site No. 3              723591 m E
a/  Detection limit 25 ppb, quantitation limit 125 ppb.
                                   75

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                             Table 41.  ANALYTICAL DATA FOR AIR SAMPLES FROM MIDLAND. MICHIGAN^-'
                                                                                              i/

Weather conditions

Samp 1 Ing
Station
1


2


3


4


5


6


U.S. Geological
Survey
coordinates
722448 m E
482819 m N

722417 m f.
4831905 m N

723591 m E
4832910 m N

725810 ra E
4831445 m N

725793 m E
4831088 ra N

726286 m E
4831833 ra N

Wind (av)
Direction
(degree)
360
245
300
360
245
300
360
245
300
360
245
315
360
245
330
360
245
285
(7 hr)
(13 hr)
(4 hr)
(6 hr)
(13 hr)
(4 hr)
(5 hr)
(13 hr)
(7 hr)
(3 hr)
(13 hr)
(8 hr)
(2 hr)
(13 hr)
(9 hr)
(8 hr)
(13 hr)
(2 hr)
Velocity
Temp.
(m/sec) (°F)
4
1
3
4
1
3
4
1
3
3
1
3
1
I
3
5
I
3
.80
.80
.00
.80
.80
.00
.40
.80
.40
.00
.80
.30
.20
.80
.80
.00
.80
.00
30-35-25
25-20-30
30-45
31-35-25
25-20-30
30-47
33-35-25
25-20-30
30-49
35-25
25-20-30
30-46
32-25
25-20-30
30-38
28-35-25
25-20-30
30-33
Sampling period
General
Partly
Clear
Partly
Partly
Clear
Partly
Partly
Clear
Partly
Partly
Clear
Partly
Partly
Clear
Partly
Partly
Clear
Partly
cloudy

cloudy
cloudy

cloudy
cloudy

cloudy
cloudy

cloudy
cloudy

cloudy
cloudy

cloudy
(total 24 hr)
4/6/77

4/7/77
4/6/77

4/7/77
4/6/77

4/7/77
4/6/77

4/7/77
4/6/77

4/7/77
4/6/77

4/7/77
1308-2025
2025-900
900-1323
1408-2025
2025-900
900-1417
1533-2025
2025-900
900-1583
1667-2025
2025-900
900-1683
1792-2025
2025-900
900-1820
1100-2025
2025-900
900-1122
Acrylamide analysis
Partlcu-
lateS/ Vaoork/
(|te/m3 ) ppb ue/m
< 1.0 < 1.0 < 1.0


< 1.0
< 1.0 < 1.0


< 1.0 < 1.0 < 1.0

< 1.0 < 1.0
< 1.0

< 1.0 < 1.0
< 1.0

< 1.0 < 1.0

< 1.0
a/  Detection limit =1.0 ug/m .
b/  Detecionn limit =1.0 ppb.

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          Table 42.  ANALYTICAL DATA FOR SOIL SAMPLES
	FOR MIDLAND, MICHIGAN	

                                                      Acrylamide
                           U.S. Geological             analysis
Sampling Station          Survey coordinates           (ppb)—

       1                      722448 m E                 < 20
                             4828198 m N

       2                      722417 m E
                             4831905 m N

       3                      723591 m E                 < 20
                             4832910 m N

       4                      725810 m E                 < 20
                             4831445 m N

       5                      725793 m E                 < 20
                             4831088 m N

       6                      726286 m E                 < 20
                             4831833 m N
a./  Detection limit 20 ppb, quantitation limit 100 ppb.
                                 77

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                              REFERENCES
1.  "Determination and Evaluation of Environmental  Levels  of  Selected
      Toxic Substances," Acrylamide, EPA Contract No.  68-01-1983,  July
      1977.

2.  "Assessment of the Need for and Character of Limitations  on Acryl-
      amide and Its Compounds," Draft Final  Report,  EPA  Contract No.
      68-01-4308, July 1977.

3.  Croll, B. T., G. M. Arkell, and R. P. J. Hodge,  Water  Research, 8_:
      989 (1974).

4.  Norris, M. V., "Acrylamide," in Encyclopedia of Industrial Chemical
      Analysis. F. D. Snell and C. L. Hilton, eds.,  Vol. 4, pp. 160-168
      (1967).

5.  Thomann, P., W. P. Koella, G. Krinke, H. Petermann,  F. Zak, and R.
      Hess, Agents and Actions, 4:48 (1974).

6.  "Sampling and Analysis of Selected Toxic Substances,"  Final Report
      No. EPA-560/6-76-021, Ethylene Dibromide,  EPA Contract  No. 68-01-
      2646.

7.  "Sampling and Analysis of Selected Toxic Substances,"  Final Report
      No. EPA-560/6-77-026, Vinylidene Chloride, EPA Contract No.  68-
      01-4115.
                                   78

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                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before completing)
1. REPORT NO.
                              2.
                                                            3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
                                                            5. REPORT DATE
  Environmental  Monitoring Near  Industrial Sites:
    Acryl amide
                                    6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
    John E.  Going
                                                            8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS

    Midwest  Research Institute
    425 Volker. Boulevard
    Kansas  City, Missouri  64110
                                                             10. PROGRAM ELEMENT NO.
                                    11. CONTRACT/GRANT NO.

                                         68-01-4115
12. SPONSORING AGENCY NAME AND ADDRESS
    Environmental Protection Agency
    Office  of  Toxic Substances
    Washington,  D.C.  20460
                                    13. TYPE OF REPORT AND PERIOD COVERED
                                         Task Final  6/77-10/77
                                    14. SPONSORING AGENCY CODE
                                         EPA-OTS
15. SUPPLEMENTARY NOTES
16. ABSTRACT
               A sampling and analysis program was conducted to determine  the levels of
    acrylamide in air, water,  soil,  and sediment 'around five industrial sites. The
    industrial sites included  monomer producers, polymer producers, and polymer users.

               Air was collected at  the plant perimeters using Chromosorb  101 adsorp-
    tion  tubes. Soil samples were collected at the  air sampling stations. Water and
    sediment  samples were taken upstream and downstream and at the plant  discharge.

               No acrylamide was found in any of the air, soil, or sediment samples.
    Acrylamide was found in only one water sample.  The level was 1,500 ppb. The
    identification of acrylamide was confirmed by GC/MS.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                               b.IDENTIFIERS/OPEN ENDED TERMS
                                                  c.  COSATl Held/Croup
    Acrylamide
    Air
    Water
    Soil
    Sediment
Sampling
Analysis
Monomer Plants
Polymer Plants
Environmental  Monitoring
Industrial  Plants
Organic
Chemistry
18. DISTRIBUTION STATEMENT
    Release Unlimited
                       19. SECURITY CLASS (This Report)
                           Unclassified
                                                   21. NO. OF PAGES
                                               20. SECURITY CLASS (This page)
                                                   Unclassified
                                                                           22. PRICE
EPA Form 2220-1 (Rev. 4-77)    PREVIOUS EDITION is OBSOLETE
                                             79

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