United States
Environmental Protection
Agency
Environmental Monitoring
Systems Laboratory
P.O. Box 93478
Las Vegas NV 89193-3478
EPA/600/8-90/086
December 1990
Research and Development
&ER&
Chromosomal Aberration
Data Analysis and
Interpretation System
User's Guide
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USER'S GUIDE
CHROMOSOMAL ABERRATION DATA ANALYSIS AND INTERPRETATION SYSTEM
Version 1.0
September 1990
Written by
Integrated Laboratory Systems
P.O. Box 13501
Research Triangle Park, NC 27709
Contract No. 68-C8-0068
Project Officer
Charles H. Nauman
Exposure Assessment Research Division
Environmental Monitoring Systems Laboratory
Las Vegas, Nevada 89193-3478
ENVIRONMENTAL MONITORING SYSTEMS LABORATORY
OFFICE OF RESEARCH AND DEVELOPMENT
U. S. ENVIRONMENTAL PROTECTION AGENCY
LAS VEGAS, NEVADA 89193-3478
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NOTICE
The information in this document has been funded wholly
or in part by the United States Environmental Protection
Agency under Contract 68-C8-0068 to Integrated Laboratory
Systems. It has been subjected to the Agency's peer and
administrative review, and it has been approved for
publication as an EPA document. Mention of trade names or
commercial products does not constitute endorsement or
recommendation for use.
DISCLAIMER
The Chromosomal Aberration Data Analysis and
Interpretation System software and documentation are provided
"as is" without guarantee or warranty of any kind, expressed
or implied. The Environmental Monitoring Systems Laboratory,
U.S. Environmental Protection Agency, Las Vegas, NV, and
Integrated Laboratory Systems will not be liable for any
damages, losses, or claims consequent to the use of this
software or documentation.
ii
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ABSTRACT
This user's manual provides guidance to researchers and
the regulatory community for interacting with a data analysis
and interpretation system, designated CA. CA is a data
management and analysis system designed for chromosomal
aberration and mitotic index data collected using in vivo test
systems. The objective in developing this system has been to
promote consistency and intercomparability of assay test
results across laboratories, thus providing researchers and
government decision makers with a means to assure comparable
analyses of test data. The CA data analysis and
interpretation system has been developed in consultation with
a panel of biostatisticians and experts in the field of
cytogenetics.
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CONTENTS
Notice ii
Abstract iii
Contents iv
Introduction 1
Part 1. Installation 3
Part 2. Operation 5
About Menus and Forms 5
CA' s Data Model 5
Entering Data and Other Information 5
The Main Menu 7
0. Leave CA 7
1. Set Up 7
1.1 Select Endpoints 7
1.2 Optional Fields 8
2. Data Entry 8
2.1 Experiment Description 8
2.2 Spreadsheet 9
3. Disk I/O 13
3.1 Recall 13
3.2 Save 15
3.3 Import and 3.4 Export 16
4. Analysis 16
4.1 Statistics 16
4.2 Graph 20
5. Utility 21
5.1 List 21
5.2 Clear Session 22
5.3 Sort 22
6. Miscellaneous (Misc.) 23
6.1 Key Field Search 23
6.2 GLP Log 24
Sample Data Sets 24
Figures 26-49
Appendix 1. Chromosomal Aberration Assay Software Development
Panel Members.
Appendix 2. Sample Test Data.
iv
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Introduction
Structural chromosomal aberrations are broadly defined as
alterations in chromosome morphology. These alterations are
assessed usually in cells at metaphase, but certain kinds of
chromosomal damage can be detected during anaphase (e.g.,
anaphase bridges) or at interphase (micronuclei). Most
chromosomal aberrations are deleterious and result in cell death.
However, some types (e.g., reciprocal translocations, small
deletions, inversions) can lead to altered gene function(s)
without an accompanying loss in cell viability. Alterations in
gene function occurring as a result of interchanges between
specific chromosome regions are correlated with the appearance of
several different kinds of cancers , indicating the probable
involvement of these events in carcinogenesis. Consistent with
this relationship, chromosomal aberrations are induced by many
known mutagens and carcinogens. These findings make the
analysis of chromosomal aberrations a useful indicator of
genotoxic damage in proliferating cell systems.
CA is a data management and analysis system designed for
chromosomal aberration and mitotic index data collected using in
vivo test systems. The software consists of a set of routines
for 1) entering, editing and storing experimental data and
descriptive information; 2) generating statistics appropriate to
the analysis of chromosomal aberrations and micronucleus index
data; and 3) presenting the results of these statistics through
graphs and tables. CA was developed in consultation with a panel
of biostatisticians and experts in the field of cytogenetics (see
Appendix 1).
This user's manual consists of two parts. The first
describes the CA Installation program provided on the program
disk. This description should be read carefully to ensure that
installation is performed correctly. The second part describes
Schwab and Amler (1990) Genes, Chromosomes and Cancer, 1,
181.
2Preston, R.J., Au, W., Bender, M.A., Brewen, J.G., Carrano,
A.V., Heddle, J.A., McFee, A.F., Wolff, S., and Wassom, J.S. (1983)
Mutat. Res. 87:143-188.
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how to use CA. The
description follows the organization of the program, describing
the main menu first, followed by the individual routines
corresponding to each of the menu entries.
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Part l: Installation
CA is intended to run on an IBM PC compatible with a hard
disk under DOS. The hard disk is assumed to be the C drive.
Throughout this manual, words bracketed by < and > signify single
keystrokes of the named key.
CA is distributed on two disks. Disk 1 contains the program
itself. Disk 2 contains the help files and sample data. Each
disk contains an installation batch file as well. Installation
is simple:
At the C:\ prompt, insert the distribution disk 1 in drive A
and type
A:INSTALL1
INSTALLl will first create the directory C:\CA and will then
copy the executable file CA.EXE into it. When this is done, you
will be prompted to insert distribution disk 2 and type
A:INSTALL2
INSTALL2 will create the C:\CA\HELP subdirectory and will
then copy the help files into it. The sample data can be
copied to the CA directory by typing at the C:\ prompt
copy A:\*.ILS C:\CA
To start CA, go to the CA directory by typing
CD\CA
CA
CA may be run from any directory or subdirectory. To install
CA in another directory, 1) copy CA.EXE into the directory from
which it is to be run, 2) make a subdirectory named HELP, and 3)
copy the help files from the distribution diskette into the new
HELP subdirectory.
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The first command changes the directory, the second begins
the program.
Initially, an introductory screen will appear noting the
program's sponsor, authors and version number, and prompting for
a user ID. If nothing appears on the screen, confirm 1) that the
CA directory exists, and 2) that CA.EXE and the HELP subdirectory
reside within it. If not, repeat the installation procedure. If
the file structure appears correct but the program will not run,
contact the Data Management Section at Integrated Laboratory
Systems (919 - 544-4589) for assistance.
After the introductory screen appears and the user's ID has
been entered, press any key to bring up the main menu (Fig. 1).
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Part 2: Operation
About Menus and Forms
The main menu is a single selection menu. Select an item by
using the arrow keys to move the highlight bar and then press
. When a selection is made, the menu closes and the
selected operation begins.
Some menus permit multiple selections. If multiple
selections are permitted, will flag or unflag the
highlighted item but will not close the menu. Multiple-selection
menus are closed with a special menu item or the ESCAPE
key.
The form screens are data entry devices. They consist of a
set of (usually labelled) fields into which text may be entered.
Selecting a field is much like selecting a menu item. To enter
text into a field, move the cursor to the field using the arrow
keys and type in the appropriate text.
Some screens are part menu and part form. Treat the menu
portion as a multiple entry menu and the form portion as a text
entry screen.
CA's Data Model
Each CA session maintains two distinct datasets. The first
is a multi-page fixed form containing descriptive information
relating to several aspects of the experiment. The second is a
spreadsheet for the experimental data, where the rows represent
observations and the columns represent fields. This spreadsheet
has a number of fixed fields and a number of optional fields that
may be selected to tailor a session to the nature of the
experiment. Help screens are available within each menu
selection through the Fl key.
Entering Data and Other Information
CA employs two line editors. The first is a very simple
editor used for the spreadsheet (except for the remarks field)
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and various parameter entry fields on some control screens.
These are short fields and their values are simply replaced.
Entering new text into these fields clears the field and the new
text appears as entered. removes characters in the
order they were entered.
Longer fields like DOS path prompts, the fields comprising
the experiment description section, and the remarks fields of the
spreadsheet and the experiment description section employ a more
complete line editor. The control keys supported are ,
, , , , and the left and right
arrow keys.
toggles between insert and overtype modes. In
insert mode, new text shifts existing text to the
right. In overtype mode, new text simply replaces the
old.
removes the character under the cursor and
shifts the remaining text to the left.
is similar to DELETE except that it removes
the character to the left of the cursor.
moves the cursor to the beginning of the field.
moves the cursor to the end of the field.
The left and right arrow keys move the cursor one
character. If the cursor is positioned under the first
character, the left arrow key will close the window and
move to the field to the left. Similarly, if the
cursor is positioned at the end of the text, the right
arrow key will close the window and move to the field
on the right.
Remarks fields are found at the bottom of each page of the
experiment description forms and on the end of each row of the
data entry spreadsheet. Moving the cursor into a remarks fields
opens a broad single line window. If the field is not empty, the
cursor moves to the end of the existing text. Any new text will
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be appended. Moving out of the window closes the window,
displaying as much of the text as the standard form field width
will allow. The hidden text is not lost and will become visible
again when the window is reopened.
The Main Menu
All but one ('Leave CA1) of the sixteen items of the main
menu (Fig. 1) are grouped into six classes: Set Up, Data Entry,
Disk I/O, Analysis, Utility, and Miscellaneous (Misc.)
0. Leave CA
This is a special item outside the six functional categories
to be described. Selecting this item will first prompt for
disposition of any unsaved changes and then return to DOS.
1. Set Up
The setup routines (Endpoints and Optional Fields) determine
which of the non-default fields will appear on the spreadsheet.
•Endpoint' allows the selection of either or both the chromosomal
aberration and the mitotic index endpoints. 'Optional Fields'
provides a way to define up to fifteen additional data fields.
1.1 Select Endpoints
This routine consists of a single screen (Fig. 2A)
presenting the two types of endpoints CA supports: Chromosomal
Aberrations and Mitotic Index. You may select either or both.
Endpoints are selected and deselected by moving the cursor
between 'Yes' and 'No1. It is not necessary to press
here. To lock in the selection and return to the main menu, move
the cursor to the 'Go' button and press . To ignore any
changes and return to the main menu, move to the 'Cancel1 button
The left and right arrow keys usually move the cursor within
the text field of the remark, but if the cursor is at either edge
of the text, the corresponding arrow key can be used to leave the
field. Note that in all cases, using an arrow key to leave a field
assumes that there is another field in the indicated direction to
move to.
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and press .
Selecting 'Chromosomal Aberrations' will activate twenty-one
columns. These include nine columns for general categories of
various types of chromosomal aberrations and twelve columns for
the distribution of aberrations among cells. Selecting 'Mitotic
Index1 will activate three columns.
1.2 optional Fields
This selection presents a subscreen consisting of four
columns and fifteen lines (Fig. 3a) with which the user may name,
describe, and type up to fifteen additional fields. A new field
is created (and subsequently appears in the spreadsheet) simply
by naming it. The only restriction on the field names is that
they may not be duplicated. 'Name 1' will appear on the first
title line of the spreadsheet, 'Name 2' will appear on the
second. There is no restriction on the description field; this
is for the user's convenience and may be omitted. The fields are
assumed to contain integers unless otherwise defined. To change
the default field types, go to the Type Field and make a
selection from the menu that appears when the field is entered
(Fig. 3b). Currently, optional fields are not used in the
statistical analysis or in the graphs. However, one category of
optional fields 'aberration1 can be used to insert specific
categories of chromosomal aberrations, not previously defined,
into that portion of the spreadsheet where such data are entered.
Data in these columns are used to calculate the locked field
•CA/cell1. Note that the 'aberration1 field type will not appear
in the field type menu unless the 'chromosomal aberration' end
point has been previously selected. returns to the main
menu.
2. Data Entry
The data entry selections (Experiment Description and
Spreadsheet) are the means by which data are entered into CA
sessions by keyboard. 'Experiment Description1 consists of a six
page form with which to enter descriptive information pertaining
to the experiment, test article, solvent, positive control, test
system, and treatment. The 'Spreadsheet1 allows access to the
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actual data.
2.1 Experiment Description
This routine consists of a six page form presented in a
double window format (Figs. 4a-4f). The left window contains the
current page of the form. To enter the descriptions, use the
arrow keys to move between fields. The right window contains an
index listing the six pages, highlighting the current page. The
form opens displaying the experiment page. Pages are changed
using the
PAGE-UP and PAGE-DOWN keys. There are no entries among these
forms that are explicitly required before statistical analysis
can be conducted, but the contents of the test agent and dose
fields are used in labelling the statistics report. If these
fields are blank, "?" will appear in their place.
2.2 Spreadsheet
The spreadsheet consists of one thousand rows, eight
permanent columns (including a remarks field), and whatever
fields were defined by the setup routines. Fifteen rows and
seven columns are visible onscreen at any one time. The arrow
keys move the cursor a single row or column at a time, adjusting
the display when an attempt is made to move outside the current
7x15 window. and shift the display up and
down a page (fifteen lines). and shift the display left and right a page (seven columns).
and move to the first and last
pages of the spreadsheet. and move to the first and
last columns of the current row. moves the cursor to the
first column of the next line.
CA assumes that the three fields ANIMAL, SLIDE, and SCORER
will be the basic key fields and provides a small window in the
lower left corner of the spreadsheet where the values of these
fields for the current line are displayed. This is for the
convenience of the user since whenever the window is shifted to
the right, at least one of the key fields is lost from view.
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Fields are limited to nine characters except for the remarks
field which will accept eighty. Entering text into the remarks
field opens a subwindow and activates the text editor. On
closing the subwindow, only the first nine characters of the
remarks text will be displayed in the spreadsheet. Each field
also has a fixed data type that is reflected in a small tag in
the lower right corner of the screen. Edits to check for illegal
values are currently installed for the integer (whole number),
real (decimal), flag (yes/no), sex (male/female), and treatment
type (treatment, control, positive-control) so attempts to enter
illegal values in these fields will be rejected. The one
exception to this is a single period, which may be entered into
any numeric field to denote a missing value.
The Sample Time field may be left blank but, if so, it must
be blank for every observation. To CA, blank sample times imply
that time is not a factor and the analyses will be conducted
accordingly. If only some Sample Time fields are blank, CA will
not know how to interpret them.
Treatment Code may be left blank. The accepted treatment
codes are 't1 for treatment group, 'p1 for positive control and
'c1 for solvent or negative control. Records with blank
treatment codes are assumed to belong to the treatment group.
Sex initially defaults to 'm1 (male), but this may be
changed with the F2 setup option described below.
As stated in section 1.1, selecting 'Chromosomal
Aberrations' while in 'Select Endpoints1 will activate twenty-one
columns in the spreadsheet. The first nine columns (plus any
additional optional columns specified for chromosomal
aberrations) are provided for entries on specific types of
chromosomal aberrations. The ninth column of this set contains
the number of aberrations per cell (excluding gaps), including
any user defined aberration column(s). Data in these columns are
not used in any of the subsequent statistical analyses conducted
on chromosomal aberration data, but are provided for the
convenience of the user. The next eleven columns of this set are
used to indicate the number of cells with 0,1,2,3...9,10 or more
aberrations. The last column contains the percentage of cells
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with at least one aberration. The user has the option of
entering data which excludes or includes information on "gaps".
Data in these columns are used in the statistical analysis to
determine if the treatment increased significantly either the
frequency of damaged cells (i.e., the number of cells with at
least one chromosomal aberration, excluding or including gaps) or
increased significantly the number of aberrations among cells
(excluding or including gaps).
When 'Mitotic Index1 has been selected, three columns are
activated in the spreadsheet. These include two columns for data
entry, one for the total number of cells scored and one for the
number of cells at metaphase. The third column contains the
mitotic index (cells at metaphase/total cells), presented as a
percentage.
The ditto character (initially the double quote "), if
entered as the first character of a field, copies into the
current field the contents of the field immediately above it, and
then moves one space down. The designated ditto character may be
toggled between the double quote and the single quote with the F2
setup option. This option can also be used to toggle the cursor
movement between down and right.
returns to the main menu.
Fl accesses the Help screens for data entry.
F2 opens a spreadsheet setup menu with nine selections
(Fig. 5a).
ASSIGN CURRENT COLUMN [fieldname] (Fig. 5b) allows you
to assign a constant value to all or part of the
current column (Fig. 5c, note the default value of
SEX). The screen consists of three lines. The first
line accepts the value to be assigned. The second
specifies the range through which the value is to be
set. The default range is from the current row to the
last non-empty row. The third line allows you to
either GO or CANCEL.
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SET DEFAULT FOR SEX TO {other-sex} toggles the default
sex. If the current default is male, {other-sex} will
be "female", and vice-versa.
CHANGE THE DISPLAY opens a screen (Fig. 5d) that allows
you to select a subset of the defined fields for
display and to modify the order in which they appear.
The defined fields are listed on the left of the
screen. Select (or deselect) fields for display with
the arrow keys and . They will appear or
disappear from a list on the right. Only the selected
fields will appear in the spreadsheet and they will
appear in the order they were selected (Fig. 5e). To
reset the default display, deselect all fields.
will do this.
MOVE {direction} AFTER DITTO allows you to toggle the
direction of cursor movement after dittoing between
down and right, {direction} is "right" if the cursor
currently moves down, and vice-versa. will
implement the change and close the setup screen.
will just close the screen.
CHANGE THE DITTO CHARACTER TO {other-character} toggles
the ditto character between single and double quote.
The double quote is default, but it requires the shift
key. If the ditto function is to be used frequently,
it may be more convenient to substitute the single
quote.
MOVE TO LINE (Fig. 5f) allows immediate movement to any
line in the spreadsheet. Selecting it opens a small
screen into which you enter a line number between 1 and
1000. You are then returned to the spreadsheet with
the cursor positioned on the indicated line at the same
column. (The pound sign # is a quick way to do this
directly from the spreadsheet).
IMPORT DATA AS ASCII FILE opens a control screen (Fig. 5g)
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from which you can name the file to be imported and the
range of lines within the spreadsheet into which the
imported data will be entered. The file to be imported must
contain data in ASCII format (simple text files) with fields
separated by one or more blanks. (Blanks within fields are
not permitted.) The fields themselves are assumed to
correspond directly to the spreadsheet the way it is
currently configured (see CHANGE THE DISPLAY above). When
•Select source file' is selected, CA will prompt you for a
search path (Fig. 5h), and then open a screen depicting all
files within the indicated path (Fig. 5i). This screen is
nearly identical to the screens that open when 'Recall' is
selected from the main menu (Fig. 6). The only difference
is that only CA data files will be listed when recalling,
but every file within the search path will be listed when
importing. To select a file from the list, highlight its
name (with the arrow keys) and . For an explanation
of how to change the search path, see Recall (Section 3.1).
When a file is selected, CA will read the data into the
corresponding columns of the spreadsheet, beginning at the
'From1 line and stopping at either the 'To1 line or the end
of the input file, whichever comes first. If the file is
locked (see Section 3.2), CA will note overwritten fields in
the log but will perform no edits. Therefore it is critical
that you make sure the data are legal and the spreadsheet is
set up correctly.
EXPORT DATA AS ASCII FILE is the inverse of IMPORT DATA.
You are again prompted for a filename and two line numbers
(Fig. 5j), but this time CA will transfer the data in the
fields defined by the spreadsheet's current configuration to
the named file.
IMPORT and EXPORT provide a convenient means to move data
between CA and other applications.
3. Disk I/O
•Recall' reads an CA file from disk and makes it the current
file. 'Save' writes the current CA file to disk. 'Import' and
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•Export', being multi-step operations, are too complicated to
execute as a single menu selection. When selected as main menu
items, 'Import1 and 'Export1 display text screens describing the
procedures.
3.1 Recall
'Recall' is the means by which existing (previously saved)
CA files are brought back into an active session. To do this,
'Recall' first identifies the CA files in the current search path
and then presents the resulting list in the main recall screen.
When 'Recall' is selected, it first offers the option of
changing the current search path (Fig. 5h). When the search path
has been defined (that is, when the default has been either
changed or confirmed), 'Recall1 conducts the search and opens the
main screen (Fig 6).
The main screen consists of five subwindows. The first and
by far the largest is the list of files in the current directory
presented alphabetically from left to right in four columns and
as many rows as necessary. In a column to the right of the file
window are 1) the drive menu, 2) the 'parent directory' button (a
button is just a one item menu), 3) the subdirectory menu, and 4)
the 'cancel1 button.
The subwindow containing the highlight bar is always the
currently active subwindow. To move between the subwindows, use
1) CTRL LEFT-ARROW, CTRL RIGHT-ARROW, CTRL PAGE-UP, and CTRL
PAGE-DOWN, or 2) TAB to cycle clockwise.
When the file list is active, the bar responds to the arrow
keys, and if the list is too long to fit in the window, PAGE-UP
and PAGE-DOWN to scroll. ENTER will select the currently
highlighted filename and close the window, returning to the point
of call.
Of the remaining subwindows, all but 'cancel1 modify the
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search path. Selecting one of the drives redefines the search
path to the root directory of the disk residing on the indicated
drive.
The 'parent1 button modifies the search path by removing the
last directory level from it.
Selecting one of the subdirectories modifies the search path
by appending the selected subdirectory to it.
If the current search path is the root directory, there will
be no parent. In this case, the highlight bar will skip the
button. Likewise, there may be no subdirectories within the
current directory. Again, in this case, the bar will skip the
appropriate button.
•Cancel1 closes the screen and performs no action.
3.2 Save
CA incorporates two file attributes, 'locking1 and 'password
protection1. These attributes can be modified only when files
are saved.
New files are initially neither locked nor protected. If
such a file is to be saved, the first menu (Fig. 7a) offers the
options LEAVE UNPROTECTED, PROTECT and CANCEL. If you select
PROTECT, you will be prompted for the password (Fig. 7b). Anyone
wishing to recall this file will have to give the password
exactly as entered here (including capitalization). If the file
to be saved is already password protected, the first menu (Fig.
7c) will offer related options: KEEP PASSWORD, CHANGE PASSWORD,
REMOVE PASSWORD, and CANCEL.
The second menu (Fig. 7d) consists of four items:
UPDATE THE CURRENT FILE can be selected only if the
current session was read from disk. If so, this option
rewrites the source file, saving any changes made
during the current session.
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CREATE A NEW FILE allows you to save the data under a
new name. If this item is selected, CA assumes the
target file does not exist. If it finds a file with
the given name, it will prompt you to OVERWRITE or
CANCEL.
OVERWRITE AN EXISTING FILE calls a screen identical to
RECALL SAVED SESSION, except that the selected file
will be overwritten by the current session. To save
disk space, files are compressed before saving.
CANCEL returns to the main menu.
Finally, if the current file is not locked, the last menu
(Fig. 7e) will consist of the items: WRITE UNLOCKED, LOCK AND
WRITE, and CANCEL. If the file is locked, any future additions
or changes will be recorded in its GLP log. Once a file is
locked, it cannot be unlocked.
3.3 Import and 3.4 Export
'Import' and 'Export1, being multi-step operations, are too
complicated to execute as single menu selections. When selected
as main menu items (as opposed to spreadsheet / F2 menu items),
•Import1 and 'Export1 open text screens describing the respective
procedures. Leaving 'Import1 and 'Export' as main menu items,
even though they cannot be executed from the main menu,
appropriately reflects their status as high-level operations.
4. Analysis
•Statistics' conducts specialized statistical analyses on
selected endpoints and presents the results via the screen with
an option to print as well. The CA software is designed to
analyze an in vivo experiment involving either one or both sexes,
from 1 to 5 scorers, and a maximum of 8 dose groups and 6 sample
times. 'Graph' plots the means of selected response variables.
4.1 Statistics
When 'Statistics' is chosen from the main menu, CA looks for
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defined fields representing cell counts suitable for analysis.
If it finds none, CA prints a warning and returns to the main
menu. If it finds entries for only a single endpoint (e.g.,
mitotic index), CA assumes analysis is to be done on this
endpoint and opens the control screen. If it finds either the
chromosomal aberration endpoint or the chromosomal aberration and
the mitotic index endpoints selected, it opens an analysis
selection menu (Fig 8a). Chromosomal aberration data can be
analyzed either as the percentage of cells containing at least
one aberration or as the total number of aberrations.
The control screen for either of the two chromosomal
aberration statistical tests (Fig. 8b) allows the user to set
certain test parameters and listing options. The screen consists
of 6 lines: (1) allows you to select the treatment or positive
control data for analysis; (2) sets the significance or alpha
level (default = 0.05); (3) allows you to select one of three
options for including or excluding the high dose from the test
(more on this below). Because the analysis of the mitotic index
is based on an ANOVA, dropping the high dose is not relevant and
this option is excluded from the control screen (Fig. 8c); (4)
gives you the option of evaluating scorer differences in addition
to time and sex (this option is not possible for positive control
data); (5) determines whether the output is to be printed as well
as listed to the screen; and (6) either initiates the statistical
analysis (GO + ) or returns to the main menu (CANCEL +
) . The current selections for these parameters are
highlighted.
When the analysis begins, each exposure group (defined by
sex, treatment and dose) is analyzed for outlier data based on
intragroup standardized residuals. If any outliers are
identified among the control, treated, or positive control
groups, a screen is opened (Fig. 8d) listing the outliers and
related information . This screen gives you the options of
printing the outliers, omitting the outliers, retaining them, or
5 Entering 'g1 at any point is equivalent to GO + .
Entering 'c1 is equivalent to CANCEL + .
6The outliers listed here were created to demonstrate the
window. They are not part of the analysis reported in the figures.
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aborting the analysis.
After the outliers, if any, have been considered, CA
presents (Fig. 8e) the variance inflation factor (a method for
taking into consideration the extent of interanimal variability
present among treatment groups), the alpha level set by the user,
the results of the initial analysis to determine whether the data
can be pooled across sexes and/or across sample times and, if
selected, the results of the scorer analysis. In the lower box,
the most appropriate model is highlighted, indicating whether the
data should be pooled across sexes, sample time, both or neither.
The user can override the initial selection or return to the main
menu.
CA uses a statistical approach appropriate to the type of
endpoint selected (i.e., for chromosomal aberration data or for
mitotic index data). Two statistical procedures for evaluating
the ability of chemicals to induce chromosomal aberrations are
available to the user. In the first analysis, called chromosomal
aberration (pet. damaged cells), for each animal tested, the
number of cells with at least one aberration (excluding or
including gaps depending on data input by the user) along with
the total number of cells scored are treated as binomial
observations. Extrabinomial variability is looked for and
quantified. Then, for control and treatment group data, ANOVA
procedures are used to test for scorer, sex and sample time
effects while a trend test is used to evaluate treatment effects.
The second analysis, called chromosomal aberration (total
aberrations), treats the total number of aberrations per animal
as Poisson observations (assuming that approximately (i.e. within
10%) the same number of cells are scored for all animals) and
performs the Poisson counterpart to the binomial analysis
discussed above. For data on the mitotic index, an ANOVA
analysis is used to evaluate for agent-induced differences in the
frequency of cells in mitosis. If the statistical analysis is
continued, the next screens (Fig. 8f) present: (i) the sample
time and sex, the calculated probability value (p) and the preset
alpha level; and (ii) summary information on the endpoint
selected, including the group mean and the standard error of the
mean for observations (Note: In this calculation, each row of
data is treated as a single observation). The results of a
18
-------
comparison of the data at each dose versus the data in the
control group (pairwise significance) is presented, with
significant values indicated by a '*'. Pressing any key allows
the user to page through each analysis, eventually being returned
to the main menu.
For a detailed description of the statistical approach used
in these analyses, see Appendix 2 of the Micronucleus Data
Management and Statistical Analysis Software7. Generally, since
the purpose of a chromosomal aberration assay is to determine
whether an agent is capable of inducing a significant increase in
either the frequency of damaged cells or in the total number of
aberrations, the resulting data should be analyzed by a one-
tailed trend test. One of the assumptions implicit to a
statistical analysis based on a trend test is that the dependent
response increases monotonically with increasing dose. However,
in a number of biological systems (including in vivo chromosomal
aberration studies), an initial increase in response, followed by
a significant downturn at higher doses, has been detected for a
small number of chemicals. To avoid the possibility that such
data would result in the improper classification of a test
chemical as being without clastogenic activity, the ability to
formally exclude data at the highest dose only from the trend
test analysis has been included in the statistical package. Of
course, the user can informally conduct the same type of analysis
by excluding data at specific doses during data entry. However,
in this case, the analysis for scorer, sex and sample time
effects would not contain the complete data set and may result in
false conclusions about the appropriateness of pooling various
data sets. If the user decides to formally incorporate the
possibility of excluding from the trend test analysis, at any
future point in time, data at the highest dose, then the preset
alpha level will be adjusted to retain the same overall beta
error rate. To accomplish this goal, the alpha level set by the
user (default level of 0.05) will be reapportioned between a
primary analysis, which would include all dose data, and a second
analysis, which would exclude data at the highest dose. The
Micronucleus Assay Data Management and Analysis System, Data
Management Systems in Genetic Toxicology, Integrated Laboratory
Systems, version 1.4, October, 1990.
19
-------
alpha for the primary analysis has been set at 80% alpha (default
of 0.04), while that for the second test has been set at 20%
alpha (default of 0.01). It would be statistically inappropriate
for the user to first analyze the data set at 100% alpha, and
then decide to exclude the data at the highest dose from the
statistical analysis. The exclusion of the highest dose from the
trend test analysis will have no effect on the analysis for
scorer, sex and sample time effects, which will utilize the total
data set.
Two methods of statistical analysis are offered for
evaluating chromosomal aberration data, one based on the
frequency of damaged cells and one based on the total number of
chromosomal aberrations among cells. The former method is the
statistical approach used most commonly. However, since the
method is insensitive for detecting agents which, for one reason
or another, selectively induce a very small number of heavily
damaged cells only, the latter method was included also in CA.
For mitotic index data, an ANOVA is used since the frequency
of cells at metaphase, depending on the duration of the
treatment, may be decreased or increased at a particular sample
time, and because the dose response often does not change
monotonically.
4.2 Graph
This selection instructs CA to plot the means of a response
variable against a classification variable with the option of
stratifying by a third variable (Fig. 9a). There is also an
option to present this information in the form of a table (Fig.
9b), either along with or instead of the graph. The response
values are assumed to be the percentage of cells with
aberrations, the frequency of aberrations per cell and the
mitotic index. The default is the first variable in this list
that is defined for the current session. The classification
variables are assumed to be 'Dose' and 'Sample Time1. The
default is 'Dose1.
The third 'by' variables are limited to 'Sex1 and 'Sample
Time'. If a 'by1 variable is selected (Sex is the default), the
20
-------
data will be stratified accordingly and presented.
Stratification means two separate means are computed and plotted
for each value of the classification variable, one for each value
of the 'by' variable.
The control screen consists of four individual submenus
(Fig. 9c). Three of these present lists of the defined fields in
the spreadsheet and are used to select the independent, the
dependent, and the classification variables respectively. The
fourth selects the type of output. The up and down arrow keys
are used to move within a submenu and the left and right arrow
keys to move between them. As with the statistics setup menus,
is not necessary except to select the 'GO1 options. The
graph presents all data listed, regardless of whether the data at
the high dose are excluded from the trend test analysis or
whether any outliers have been detected and excluded from the
statistical analysis. By manually removing selected data from
the spreadsheet, the user can exclude these data from the graph.
Use to make hard copies of the graphs and
tables.
5. Utility
'List1 produces a hard copy listing through a printer
connected to the serial port. There are a number of parameters
with which the user can determine the form of the report. 'Sort'
reorders the lines of the spreadsheet (and optionally subsequent
listings) according to the values of any combination of key
fields, either ascending or descending. 'Clear Session' deletes
all data and removes all Set Up fields, in effect starting over.
5.1 List
CA can generate a hard copy listing of either the experiment
description, the data or both. A preliminary menu (not shown)
presents this choice. A control screen (Fig. lOa) governs
several aspects of the list:
a) The first three lines allow the user to indicate
21
-------
whether the list is to be sorted and, if so, in what
order. See the Section 5.3 below on sorting the data
for more information.
b) The next group consists of two lines that control
the range of lines to be printed. The default is
•print to the last non-empty line1, but this can be
overridden on the second line by typing a range of
lines into the fields.
c) Similarly, CA will by default print all fields. To
change this, select the second of the next group of
lines 'Select Subset1. This will open a window (Fig.
lOb) in which all the defined fields are listed on the
left and a blank list to be printed is displayed on the
right. Select (or deselect) fields for printing with
the arrow keys and (they will appear or
disappear from the list on the right). The list on the
left does not change except for small arrows that
appear beside the names of the currently selected
fields. Press to return to the control page.
d) The next two lines, 'Lines per page' and 'Columns
per page' govern page length and width. These should
be adjusted only if the printer text is set to a
smaller size.
e) The last line contains the commands PRINT which
begins the printing sequence and CANCEL which returns
to the main menu. There is currently no way to stop
printing short of restarting the equipment.
5.2 Clear Session
This is an easy way of starting over. All data and session
configurations are erased. There is no screen associated with
the selection. To prevent accidental erasure of unsaved changes,
the user will be prompted by "Are you sure? Yes or No".
22
-------
5.3 Sort
CA can order the rows of the data spreadsheet based on the
values of certain key fields. The user must specify what the key
fields are, what their priority is to be, and whether the records
should be sorted by ascending or descending values. The sort
screen (Fig. 11) lists the fields defined for the current session
in a box on the left and the currently defined key fields in a
box on the right. Initially, the second list is empty.
Key fields are selected and deselected with the arrow keys
and . If the records are to be sorted by descending
values (from largest to smallest) use the '-' key instead of
. Descending keys are marked with a downward pointing
arrow to their left. To change a currently selected key from
ascending to descending, highlight its name and type '-'. To
change it from descending to ascending, type '+'.
The order in which the keys appear in the 'selected keys'
list on the right reflects their priority. The records will be
sorted on the first key field and ties will be resolved on the
second and so on. Any ties remaining when the keys have all been
used will remain, but not necessarily in their original order.
The order in which the key fields are defined is critical since
there is no way to change the order of keys once they are
selected. To insert a key anywhere other than at the end of the
list, it is necessary to deselect then reselect the existing
keys. Sometimes it is easiest to clear the list and begin again.
does this.
When the key fields have been selected, use to
begin the sort. In sorting, CA does not physically reorder the
records but rather builds an index that determines a records
apparent location. To remove the index, select SORT and
the key fields.
6. Miscellaneous (Misc.)
'Key Field Search1 produces a report listing either 1) the
values of selected fields for all CA files in the indicated
search path, or 2) the names of all CA files in the indicated
23
-------
search path whose values for selected fields match key values
entered here. 'GLP Log1 presents by screen or printer a record
of all accesses to the file and, after locking, all new entries
or changes to existing entries, who made them and when.
6.1 Key Field Search
A key field file search will either a) list selected
experiment description fields for all CA files in a given search
path, or b) list all files in the current search path whose
values for the selected fields match user defined target values.
The initial screen (not shown) allows you to select one of
these options. If you elect to simply list key fields, a screen
will open identical to that used to select fields for listing or
reconfiguring the spreadsheet. When the fields to list have been
selected, you will be given the chance to redefine the
searchpath. When the search has been completed, you may elect to
list the results or view them onscreen as with the GLP log
report.
If, on the other hand, you elect to list the names of files
with matching key field values, selecting a field opens a
subwindow (Fig. 12a) into which you enter the target string.
When the target string is defined, a subwindow opens (Fig. 12b)
prompting you for a match criteria (e.g. whether the field is to
exactly match the target string or is only to contain the target
string). The third option, a partial match, is not yet
implemented.
6.2 GLP Log
To further compliance with good laboratory practices, CA
maintains a log as part of each file recording the time and date
of each access and the ID entered at the beginning of the current
ses-sion. If a file has been 'locked' (see Section 3.2), then
this log will record all additional entries and changes to
existing entries.
The log may be viewed onscreen or printed.
24
-------
Sample Data Sets
To assist the user in becoming familiar with CA, data from
two sample experiments (CATEST01.ILS and CATEST02.ILS) have been
included (Appendix 2). Both experiments present data in which
male and female mice were treated once with a test chemical, bone
marrow samples collected at 24, 48 and 72 hours after treatment
and the frequency and distribution of chromosomal aberrations and
the mitotic index were determined by two scorers. In CATEST01,
the chromosomal aberration data includes cells containing 0, 1 or
2 aberrations. In CATEST02, cells containing multiple
aberrations were detected among the treated mice. The same
outlier data are included in both data sets. The statistical
analyses of the chromosomal aberration data are based on the
assumption that excluding the data at the highest dose would
always be considered and that scorer differences would be
evaluated. Finally, the trend test analysis was conducted (i)
without pooling data obtained on males and females or at the
three sample times, and (ii) pooling data, where appropriate,
across sexes and sample times. The corresponding hard copies of
the experimental information, the chromosomal aberration and
mitotic index data and the results of the various statistical
analyses are provided in Appendix 2. It is suggested that the
user recall the sample test data files, page through the various
screens and invoke each of the subroutines. It may also be
useful to modify the entries and rerun the menu options.
We sincerely hope that you will find this program both
useful and friendly. We welcome any suggestions or comments you
might have. Please write, call, or FAX to:
EPA Software Development Project
Integrated Laboratory Systems
Genetic Toxicology Program
P.O. Box 13501
Research Triangle Park, NO 27709
(919) 544-4589
25
-------
— Setup
Endpoints
Optional
Fields
•Data Entry-
Experiment
Description
Spreadsheet
Leave CA
rDisk I/O
Recall
Save
Export
**
—Analysis-
Statistics
Graph
rUtility
List
Clear
session
Sort
4+
— Misc. — n
Key Field
Search
GLP Log
Return to DOS.
Select Endpoints
Chromosomal Aberrations: No Yes
Mitotic Index: No Yes
Go
Cancel
Figures 1 & 2
26
-------
Label 1
1 Userl
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Define up to fifteen additional fields
Label 2 Description
(any description here)
Type
int
Define up to fifteen additional fields
Label 1
1 Userl
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Label 2 Descripti9n
(any description here!
Define value type for field [Userl] n
integer (whole number)
real (decimal)
character (string)
date (string)
flag (y/n)
sex (m/f)
aberration (whole number)
Arrow keys move the selector arrow.
RETURN / ENTER to select.
Type
int
Figures 3a & 3b
27
-------
EXPERIMENT
Record
Laboratory
Lab Book
Date Started
Date Completed ....
Slides Coded By ...
Staining Method . . .
Scored By
Entered By
Entry Date
Proofed By
CA-TEST 01
121
ILS
III
06/18/90
08/01/90
MP
GIEMSA
CJH
MP
07/25/90
DC
Experiment
Test
Article
Vehicle
Positive
Control
Test
System
Treatment
Main
Menu
TEST ARTICLE
Receipt Date
CAS #
Source / Lot
Appearance
Purity
stability
Storage Conditions
Solubility
Hazard Information
067889
04/20/90
UNKNOWN
RADIAN 067H3_
ORANGE POWDER.
UNKNOWN
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER_
TREAT AS CARCINOGEN.
Remarks
Experiment
Test
Article
Vehicle
Positive
Control
Test
System
Treatment
Main
Menu
-PgDn-
Figures 4a & 4b
28
-------
VEHICLE
Source / Lot
Purity
Stability
Storage Conditions
CORN OIL
SIGMA/13F100
UNKNOWN
1 YEAR
ROOM TEMPERATURE
Remarks
Experiment
Test
Article
Vehicle
Positive
Control
Test
System
Treatment
Main
Menu
-PgDn-
POSITIVE CONTROL ..
Receipt Date
CAS #
Source / Lot
Appearance
Purity
Stability
Storage Conditions
Solubility
Hazard Information
991870
01/22/90.
UNKNOWN
RADIAN/110BD
WHITE POWDER.
UNKNOWN
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER.
CARCINOGEN
Remarks
Experiment
Test
Article
Vehicle
Positive
Control
Test
System
Treatment
Main
Menu
-PgDn-
Figures 4c & 4d
29
-------
TEST SYSTEM
Species
Strain
Received
Quarantined From
Until
MOUSE
B6C3F1
TACONIC FARMS
05/01/90
5/01
5/15_
Routine Husbandry Conditions? (y/n) Y
Sex
M
F
Age units
Remarks
Age Weight
Fr. To Fr. To
11 11 32.0 34.0
11 11 27.0 29.0
WEEKS Weicrht units GRAM
Experiment
Test
Article
Vehicle
Positive
Control
Test
System
Treatment
Main
Menu
TREATMENT
Date Started
Date Completed . . .
Route
Volume
Doses
Dose Units
Number Treatments
Treatment Duration
Treatment Date . . .
Treatment Interval
Number Samples . . .
Sample Date
Sample Interval . .
Tissue Cell Type
06/18/90
06/21/90
IP
0.4
0, 500, 1000, 2000
MG/KG
1
NA
06/18/90
NA
3
06/19:06/20:06/21
24 HR
BONE MARROW
Experiment
Test
Article
Vehicle
Positive
Control
Test
System
Treatment
Main
Menu
Figures 4e & 4f
30
-------
Esc -» Main Menu
Fl - Help
F2 - Setup
Scorer
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Treat.
Code
Sex
Dose
Sample
Time
Number
Cells
chr-tid
gap
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
c m 0 24 25
Assign current column [Scorer]
Set default for SEX to female
Change the display
Move right after ditto
Change the ditto character to single quote ( ' )
Move to line
Import data as ASCII file
Export data as ASCII file
Cancel
t m 1000 24 25
t m 1000 24 25
0
0
0
0
0
0
0
0
0
1
0
0
1
Animal
Slide
Scorer
1054
A
MP
character
Esc -* Main Menu
Fl - Help F2 - Setup
1 _
2
3
4
5
6 _
7
8
9
10
1 1
12
13
14 _
15
Animal
Slide
Scorer
Scorer Treat. Sex
Code
MP c m
MP — —
... MP
MP
MP
MP
MP
MP •
MP
MP
MP
Set Scorer
From row [
» Go «
MP t m
MP t m
MP t m
MP t m
1054
A
MP
Dose
0
to [
1] to row
» Cancel
1000
1000
1000
1000
Sample Number
Time Cells
24 25
DC]
[ 10]
«
24
5
5
5
5
5
5
5
5
5
5
25
24 25
24 25
24 25
chr-tid
gap
0
1
0
0
0
0
0
0
0
0
1
0
0
0
1
Figures 5a & 5b
character
31
-------
Jsc •* Main Menu Fl •* Help F2 -> Setup
Scorer Treat. Sex Dose Sample Number chr-tid
Code Time Cells gap
1 DC c m 0 24 25 0
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Animal
Slide
Scorer
DC
DC
DC
DC
DC
DC
DC
DC
DC
MP
MP
MP
MP
MP
1054
A
DC
c
c
c
c
t
t
t
t
t
t
t
t
t
t
m
m
m
m
m
m
m
m
m
m
m
m
m
m
0
0
0
0
500
500
500
500
500
1000
1000
1000
1000
1000
24
24
24
24
24
24
24
24
24
24
24
24
24
24
25
25
25
25
25
25
25
25
25
25
25
25
25
25
1
0
0
0
0
0
0
0
0
1
0
0
0
1
character
Figure 5c
32
-------
-Animal
-Slide
-Scorer
Treat. Code
Sex
-Dose
-Sample Time
Number Cells
chr-tid gap
chr-some gap
chr-tid break
chr-some break
chr-tid exchange
chr-some exchange
other aberratn
CA/cell exc gap
cells w/ 0 CA
cells w/ 1 CA
cells w/ 2 CA
cells w/ 3 CA
!••• i i more I =
Animal
Slide
Scorer
Sample Time
Dose
[t] [i] [PAGE UP] [PAGE DOWN] [ENTER] [DELETE] [ESCAPE] [Fl = help]
Esc - Main Menu Fl - Help F2 - Setup
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Animal
Slide
Scorer
Animal
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1054
A
DC
Slide
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
Scorer
DC
DC
DC
DC
DC
DC
DC
DC
DC
DC
MP
MP
MP
MP
MP
Sample
Time
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Dose
0
0
0
0
0
500
500
500
500
500
1000
1000
1000
1000
1000
Figures 5d & 5e
character
33
-------
Esc - Main Menu Fl - Help F2 - Setup
1
2
3
4
5
g
7
8
9
10
11
12
13
14
15
Animal
Slide
Scorer
Animal Slide Scorer Sample
Time
1054 A DC 24
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1054
A
DC
A DC
A DC
24
24
Dose
0
0
0
Go to line:
A DC
A DC
A DC
A DC
A MP
A MP
A MP
A MP
A MP
24
24
24
24
24
24
24
24
24
500
500
500
500
1000
1000
1000
1000
1000
character
Figure 5f
34
-------
Select source file
start with line 1
stop with line 1000
Go
Cancel
C:\CA\testdata\_
search path
Figures 5g & 5h
35
-------
CATEST01.ILS
DELETE.ME
TESTDATA.CA2
Contents of C:\CA\testdata\
CATESTO1.TXT CATESTO 2.1LS
EXPINFO OUTFILE.NEW
CATEST02.TXT
TESTDATA.CA1
— Drives n
A B C D I
Parent
- n
I
Directories —,
Cancel
Import C:\CA\testdata\ CATESTOl.ILS
Export data to file:
C:\CA\testdata\ascii.out
start with line 1
stop with line 260
Go
Cancel
Figures 5i & 5j
36
-------
CATEST01.ILS
TESTDATA.CA1
Contents of C:\CA\testdata\
CATEST02.XLS DELETE.ME
TESTDATA.CA2
EXPINFO
— Drives
A B C D
Parent
- n
I
Directories —i
Cancel
Recall C:\CA\testdata\ CATEST01.ILS
Figure 6
37
-------
Password protection
Leave unprotected Protect Cancel
Password?
Figures 7a & 7b
38
-------
Password protection
Keep password Change password Remove password Cancel
Update the current file
Create a new file
Overwrite an existing file
Cancel
Current file: C:\CA\testdata\CATEST01.XLS
Figures 7c & 7d
39
-------
Write unlocked Lock and write Cancel
Write to file
C:\CA\testdata\CATEST01.ILS
Figure 7e
40
-------
» Select endpoints for analysis «
Chromosomal aberration (pet. damaged cells)
Chromosomal aberration (total aberrations)
Mitotic index
Cancel
Figure 8a
41
-------
Analysis Group:
Significance:
Omit the high dose:
Evaluate scorer differences:
Print report:
Go
Treatment
0.050
Never
No
No
Positive Control
Maybe Yes
Yes
Yes
Cancel
Analysis Group:
Significance:
Evaluate scorer differences:
Print report:
Treatment
0.050
No
No
Go
Positive Control
Yes
Yes
Cancel
Figures 8b & 8c
42
-------
There are 2 outliers at the .05 significance level.
Line Dose Tine Sex
1 3 0.0 24.0 m
2 133 0.0 24.0 m
Pet. Mean Pet.
60.000 13.600
80.000 17.600
Omit outliers Keep outliers
Print outliers Cancel
Endpoint: Chromosomal aberration (pet. damaged cells)
Variance inflation factor:
Alpha:
1.000
0.050
Factor
Scorer
Sex
Time
Deviance
30.014
8.226
30.904
df
24 0.1843
12 0.7673
8 0.0001
Select trends option
Collapse on Sex Time Both Neither
= Best choice: SEX =
Cancel
Figures 8d & 8e
43
-------
Time
P
alpha
24.00 (Both sexes) for 067889
0.000
0.050
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant Cells Percent SEM
MG/KG Cells Scored Aberrant (for Obs)
0.00 7 450 1.5556 0.5729
500.00 15 500 3.0000 0.6407
1000.00 28 500 5.6000 0.8897
2000.00 56 500 11.2000' 1.1462
Hit any key ..
Pairwise
Significance
0.0697
0.0005
0.0000
*
*
Time = 48.00 (Both sexes) for 067889
p = 0.000
alpha = 0.050
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
6
37
61
81
Hit any key ..
Cells Percent
Scored Aberrant
500 1.2000
500 7.4000
500 12.2000
500 16.2000
SEM
(for Obs)
0.5109
1.3067
1.5755
2.1418
Pairwise
Significance
0.0000
0.0000
0.0000
Figure 8f
44
-------
Average number of aberrations per cell
0.16 -|
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0.00
C
B
C
B
A C
B
A
C
A
BC
) 500 1000 2000
Dose
Stratified by Sample Time A = 24.00
ENTER -» continue
B = 48.00
72.00
Average number of aberrations per cell
For sample time =
Dose 0.00
500.00
1000.00
2000.00
24.00
0.084
0.030
0.056
0.112
48.00
0.012
0.074
0.122
0.162
72.00
0.012
0.040
0.086
0.148
Figures 9a & 9b
45
-------
Graph Setup
Independent
Dependent
By
Report
Dose
Sample Time
CA/cell exc gap
pet. CA positive
MI percent
Sex
Sample Time
Do not stratify
Graph & table
Graph only
Table only
Cancel
Select to Go
Figure 9c
46
-------
Print unsorted list
Build new index and print
Print with current index
Print all lines
Print from line [
Print all fields
Select subset
Lines per page [
Columns per page [
1] to line [ 260]
50]
7]
Print
Cancel
—Animal
->Slide
-Scorer
Treat. Code
Sex
-•Dose
-Sample Time
-Number Cells
chr-tid gap
-chr-some gap
—chr-tid break
-chr-some break
-chr-tid exchange
-chr-some exchange
-other aberratn
-CA/cell exc gap
cells w/ 0 CA
cells w/ 1 CA
cells w/ 2 CA
cells w/ 3 CA
more l ==
Animal
Slide
Scorer
Dose
Sample Time
Number Cells
chr-some gap
chr-tid break
chr-some break
chr-tid exchange
chr-some exchange
other aberratn
CA/cell exc gap
[T] [I] [PAGE UP] [PAGE DOWN] [ENTER] [DELETE] [ESCAPE] [Fl
Figures 10 a & lOb
help]
47
-------
-•Animal
-Slide
"Scorer
Treat. Code
Sex
-Dose
-Sample Time
Number Cells
chr-tid gap
chr-some gap
chr-tid break
chr-some break
chr-tid exchange
chr-some exchange
other aberratn
CA/cell exc gap
cells w/ 0 CA
cells w/ 1 CA
cells w/ 2 CA
cells w/ 3 CA
I more i =====
TAnimal
TSlide
TScorer
ISample Time
iDose
[T] [i] [PAGE UP] [PAGE DOWN] [ENTER] [+] [-] [DELETE] [ESCAPE] [Fl = help]
Figure 11
48
-------
EXPERIMENT
Record
Laboratory
Lab Book
Enter search string for Experiment / EXPERIMENT
ILS
Experiment
EXPERIMENT
Record
Laboratory
Lab Book
Enter search string for Experiment / EXPERIMENT
Search string
ILS
Search string matches the key field
Search string is included within the key field
Search string partially matches the key field
Cancel
Experiment
Figures 12a & 12b
49
-------
APPENDIX 1
CHROMOSOMAL ABERRATION ASSAY SOFTWARE DEVELOPMENT
PANEL MEMBERS
-Dr. James Allen
U.S. Environmental Protection Agency
-Dr. David H. Blakey
Health and Welfare Canada
-Dr. Michael Cimino
U.S. Environmental Protection Agency
-Dr. Russell J. DuFrain
Bristol-Myers Co.
-Dr. Ed Frome
Oak Ridge National Laboratories
-Dr. Osamu Hirai
Fujisawa Pharmaceutical Co. Ltd.
-Dr. Hank E. Holden
Pfizer Inc.
-Dr. Graham Hook
UT-Oak Ridge National Laboratories
-Dr. Andrew Kligerman
U.S. Environmental Protection Agency
-Dr. James MacGregor
Toxicology Consultants Services, Inc.
-Dr. Barry H. Margolin
University of North Carolina
-Dr. Al F. McFee
Oak Ridge National Laboratories
-Dr. Robert Naismith
Biofor, Inc.
-Dr. Charles H. Nauman
U.S. Environmental Protection Agency
-Dr. R. Julian Preston
Oak Ridge National Laboratories
-------
-Dr. Michael F. Salamone
Ministry of the Environment, Canada
-Dr. Juan San Sebastian
Parmakon Research International
-Dr. Elizabeth S. Von Halle
Oak Ridge National Laboratories
-Dr. Deiter Wild
University of Wuerzburg
-Dr. Llewellyn R. Williams
U.S. Environmental Protection Agency
-------
APPENDIX 2
SAMPLE TEST DATA
-------
SAMPLE TEST DATA
CATEST01
-------
C:\CA\CATEST01.ILS listed at on
EXPERIMENT CA-TEST 01
Record 121
Laboratory ILS
Lab Book Ill
Date Started 06/18/90
Date Completed .... 08/01/90
Slides Coded By ... MP
Staining Method ... GIEMSA
Scored By CJH
Entered By MP
Entry Date 07/25/90
Proofed By DC
Remarks
TEST ARTICLE 067889
Receipt Date 04/20/90
CAS # UNKNOWN
Source / Lot RADIAN 067H3
Appearance ORANGE POWDER
Purity UNKNOWN
Stability 1 YEAR
Storage Conditions ROOM TEMPERATURE
Solubility INSOLUBLE IN WATER
Hazard Information TREAT AS CARCINOGEN
Remarks
-------
VEHICLE ,
Source / Lot ,
Purity
Stability
Storage Conditions
Remarks
CORN OIL
SIGMA/13F100
UNKNOWN
1 YEAR
ROOM TEMPERATURE
POSITIVE CONTROL ..
Receipt Date
CAS f
Source / Lot
Appearance
Purity
Stability
Storage Conditions
Solubility
Hazard Information
Remarks
991870
01/22/90
UNKNOWN
RADIAN/110BD
WHITE POWDER
UNKNOWN
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER
CARCINOGEN
-------
TEST SYSTEM
Species
Strain
Supplier ...
Received ...
Quarantined
From
Until
MOUSE
B6C3F1
TACONIC FARMS
05/01/90
5/01
5/15
Routine Husbandry Conditions? (y/n) Y
Age
Sex Fr. To
M 11 11
F 11 11
Age units WEEKS
Weight
Fr. To
32.0 34.0
27.0 29.0
Weight units GRAM
Remarks
TREATMENT
Date Started
Date Completed ...
Route
Volume
Doses
Dose Units
Number Treatments
Treatment Duration
Treatment Date ...
Treatment Interval
Number Samples ...
Sample Date
Sample Interval ..
Tissue Cell Type
06/18/90
06/21/90
IP
0.4
0, 500, 1000, 2000
MG/KG
1
NA
06/18/90
NA
3
06/19;06/20;06/21
24 HR
Remarks
-------
Animal Slide Scorer Treat. Sex Dose Sample Nunber chr-tid chr-some chr-tid
Code Time Cells gap gap break
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
1054|
1055 j
1056 j
1057J
1058 j
1059 j
1060J
1061 j
1062J
1063J
I
1064 1
1065 1
1066 j
1067)
1068 j
1069)
1070 j
1071 1
1072 j
1073 1
I
1074 1
1075 1
1076|
1077|
1078)
1079J
1080)
1081 1
1082 1
1083|
1
1084|
1085 j
1086 j
1087)
1088 j
1089 1
1090 j
1091 1
1092 j
1093 1
1
1094 1
1095J
1096 1
1097|
1098 1
1099|
1100|
1101J
1102 j
1103 j
I
I
A|
A|
A|
A|
Al
A|
Al
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
Al
A|
Al
A|
Al
A|
A|
A|
A|
HP) C
HP | C
HPJ C
HP | C
HP | C
HPJ t
MPJ t
HP | t
NPJ t
MPJ t
I
HP | t
HP | t
MPJ t
HP | t
HPJ t
MPJ t
MPJ t
MPJ t
HP | t
HP | t
I
HP) C
MPJ C
MPJ C
MPJ C
HPJ C
HP | t
MP| t
MP| t
MP| t
MP| t
I
MP| t
MP| t
MP| t
MP| t
MP| t
MP| t
MP| t
MP| t
HP | t
HP | t
I
MP| C
MP| C
HP | C
HP | C
HP | C
HP | t
HP| t
HP| t
MP| t
HP| t
I
I
I
I
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m
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m
I ™
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m
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m
m
m
m
n,
m
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m
n,
n,
m
m
m
m
m
m
m
m
m
m
m
m
m
m
m
m
m
m
0 1
0 1
0 1
o|
o|
500 1
500 1
500 j
500 1
500 1
I
1000 1
1000 1
1000 j
1000 j
1000 j
2000 j
2000 j
2000 1
2000 1
2000 1
I
0|
0|
0|
0|
o|
500 1
500 1
500 1
500 1
500 1
I
1000 1
1000 j
1000 j
1000 j
1000 j
2000 1
2000 1
2000 1
2000 1
2000)
I
o|
o|
o|
o|
"I
500 1
500 1
500 1
500 1
500 1
I
I
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
I
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
I
48|
48|
48 1
48 1
48 1
48 1
48|
48 1
48 1
48 1
I
48 1
48|
48 1
48|
48 1
48 1
48|
48 1
48 1
48 1
I
n\
n\
n\
72|
72|
72|
n\
n\
n\
72!
I
I
25 1
25 1
25 1
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25 1
25 1
25 1
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25 1
25 1
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25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25|
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
'5 1
25 1
25 1
I
I
0|
M
0|
0|
0|
Q I
Q I
0|
Q I
Q |
I
1|
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Q |
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11
I
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Q |
0 1
oj
'I
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0|
1
3|
0 1
0 1
11
0|
3|
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0|
2|
1
1|
1|
0 1
Q I
Q j
°l
°l
°l
°l
M
I
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0|
0|
0 1
Q |
0|
0|
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Q |
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0|
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0|
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0|
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Q I
0|
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0|
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Q I
Q 1
o|
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o|
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0|
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0|
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0|
0|
o|
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0|
0|
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0|
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0|
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0|
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11
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0|
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11
11
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3|
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1)
0|
11
°\
0|
11
11
I
I
-------
1
2
3
4
5
6
7
8
9
10
11
12
13
U
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
-8
49
50
chr-som
break
o|
o|
o|
o|
0|
o|
°l
0|
o|
o|
I
0|
0|
o|
"I
o|
o|
o|
°l
o|
0|
I
o|
o|
°l
o|
o|
o|
o|
o|
o|
o|
I
0|
o|
o|
o|
o|
o|
o|
0|
o|
o|
I
°l
o|
°l
o|
o|
o|
o|
°l
o|
o|
I
I
chr-tid
exchange
o|
o|
°l
o|
°l
°l
0|
°l
"I
o|
I
°l
0|
"I
o|
o|
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o|
o|
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o|
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chr-sotne
exchange
°l
0|
o|
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0|
0|
0|
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0|
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0|
0|
0|
0|
0|
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0|
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0|
0|
0|
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0|
0|
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o|
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°l
0|
0|
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0|
0|
0|
°l
I
I
other
sberratn
0|
0|
0|
0|
°l
"I
«l
o|
0|
0|
I
0|
0|
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°l
o|
o|
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0|
0|
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0|
0|
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0|
0|
0|
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0|
0|
°l
«l
o|
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«l
0|
0|
0|
0|
0|
Q|
0|
°l
o|
CA/cell
exc gap
o|
o|
0.600 |
0.040J
0.040J
o|
"I
o|
0.040]
0.040)
I
0.040)
0.080 |
0.040J
o|
0.040|
0.120 j
0.160 |
0.120 j
0.080 j
0.040 |
I
o|
0.040|
«l
0|
0.040|
0.040 j
0.080 |
0|
0.040|
0.040 |
I
0.120|
0.120 1
0.080 |
0.200 j
o|
0.240J
0.120)
0.080)
0.280 j
0.200)
I
0|
0|
o|
C.040)
0|
0.040)
o|
0|
0.040)
0.040|
I
I
cells w/
0 CA
25)
25)
10)
24)
24 1
25)
25)
25 1
24 1
24 1
I
24)
S|
24)
25)
24)
22)
21 1
22)
23)
24)
I
25)
24)
25)
25)
24)
24)
23)
25)
, 24)
24)
I
22)
B |
22 1
20 1
25)
19)
22|
23)
19)
20)
I
25 1
25 1
25 1
24)
25)
24)
25)
25)
24 1
24 1
I
I
cells w/
1 CA
0)
°l
15)
1|
11
o|
0|
0|
1|
1|
1
1|
2|
M
0)
M
3|
«l
3)
2)
1)
I
0|
11
o|
0|
11
11
2)
0)
11
1|
1
3)
2|
3|
5|
"1
*l
3|
2|
5|
5|
1
0|
"1
0|
M
0|
11
0|
0|
M
'I
'I
I
cells w/
2 CA
°l
o|
0|
o|
0|
0)
0|
°l
o|
o|
I
o|
o|
"I
«l
o|
0|
°l
"I
0|
0|
I
o|
»l
0|
0|
o|
o|
o|
0)
0|
0|
I
o|
0|
o|
o|
0|
0|
"I
0|
M
o|
I
o|
0|
o|
<>l
»l
0|
o|
0|
°l
0|
I
!
cells w/
3 CA
0|
0|
°l
0|
0|
»l
o|
°l
«l
o|
I
0|
0|
o|
-------
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
cells u/
6 CA
0|
o|
o|
o|
o|
0|
0|
°l
0|
0|
I
°l
o|
o|
0|
o|
o|
0|
0|
o|
0|
I
o|
o|
o|
0|
0|
o|
0|
o|
»l
o|
I
o|
0|
0|
o|
0|
0|
0|
0|
o|
0|
1
1
01
0|
o|
o|
o|
0|
o|
0|
0|
o|
1
1
cells w/
7 CA
«l
o|
o|
o|
°l
o|
0|
"I
o|
o|
1
o|
0|
o|
°l
o|
o|
"1
o|
o|
o|
1
0|
0|
o|
0|
°l
°l
0|
0|
0|
o|
1
°l
°l
0|
o|
0|
0|
°l
°l
o|
o|
1
1
o|
0|
»l
0|
0|
o|
°l
°l
0|
o|
1
1
cells u/
8 CA
o|
0|
°l
o|
o|
0|
°l
«l
o|
o|
1
0|
o|
PI
0|
o|
o|
o|
o|
o|
o|
1
«l
o|
o|
o|
o|
o|
0|
0|
0|
0|
1
o|
o|
o|
o|
0|
0|
0|
°l
0|
o|
1
1
o|
o|
«l
0|
"I
'-\
°l
o|
o|
0|
1
1
cells M/
9 CA
0|
0|
°l
0|
»l
0|
o|
°l
o|
0|
1
»l
"1
°l
»l
o|
«l
o|
0|
°l
0|
1
0|
°l
0|
«l
0|
0|
0|
°l
0|
o|
1
0|
o|
"I
o|
0|
0|
0|
o|
°l
0|
1
1
0|
0|
°l
o|
0|
°l
o|
°l
0|
0|
1
1
cells w/
10* CA
o|
0|
0|
0|
0|
0|
o|
0|
"1
0|
1
o|
o|
o|
0|
0|
0|
o|
o|
o|
o|
1
0|
0|
o|
«l
0|
0|
o|
0|
°l
0|
1
°l
0|
0|
o|
0|
0|
o|
0|
0|
c|
1
1
o|
0|
°l
°l
0|
o|
3|
°l
0|
0|
1
1
pet. CA
positive
0.0|
0.0|
60. OJ
4.0|
4.0|
o.oj
o.oj
o.oj
4.0|
4.0|
1
4.0|
8.0|
4.0|
0.0|
4.0|
12. 0|
16. OJ
12. OJ
8.0J
4.0|
I
0.0|
4.0|
0.0|
o.oj
4.0|
4.0)
8.0J
o.oj
4.0|
4.0|
I
12.0|
8.0J
12.0J
20. OJ
o.oj
24. OJ
12. 0|
8.0J
24. OJ
20. OJ
i
1
0.0|
0.0|
0.0|
4.0|
o.ol
4.0|
0.0|
o.oj
4.0|
4.0|
1
1
HI
* cells
500)
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 1
500 j
500 j
1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 1
i
1
500 1
500 j
500 j
500 1
500 1
500 1
500 1
500 j
500 j
500 j
1
1
MI
# meta
«|
«l
21 1
10|
»l
25 1
33 1
14|
18|
«l
I
16|
25 1
24 1
21 1
«l
16|
25 1
14|
25 1
24 1
I
25|
28|
32 1
18|
19|
21 1
24 1
18|
17|
19|
I
15|
14 1
«|
15|
18|
10|
»l
5|
•I
7\
i
1
25 1
32 1
18|
12|
15|
18|
19|
21 1
22 1
«|
MI
percent
3.00J
2.40)
4.20J
2.00J
1.80J
5.00J
6.60J
2. 80 j
3.60J
3.00J
I
3.20|
5.00J
4. 80 j
4.20J
3. 00 j
3. 20 j
5.00J
2.80J
5. 00 1
4. 80 1
I
5.00|
5. 60 j
6. 40 j
3.60J
3. 80 1
4. 20 1
4.80J
3. 60 1
3.40J
3. 80 1
I
3.00|
2.80J
2.60J
3. 00 1
3.60J
2.00J
1.80 1
1.00 j
1.60 j
1.40J
i
1
S.OOj
6.40J
3.60|
2.40J
3.00J
3.60J
3. 80 1
4.20J
4.40J
3.00|
1
1
-------
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
Animal Slide
1104)
1105 1
1106 1
1107J
1108 1
1109)
1110J
1111J
1112J
1113J
I
1114|
1115J
1116J
1117J
1118J
1119J
1120 j
1121 1
1122 j
1123)
I
1124|
1125|
1126|
1127|
1128|
1129|
1130 1
1131 j
1132 j
1133 j
I
1134|
1135|
1136|
1137|
1138|
1139|
1140|
1141|
1142|
1143|
1
1144|
1145|
1146|
1147J
1148|
1149|
1150|
1151|
1152J
1153|
1
1
*l
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
*l
A|
A|
A|
A|
A|
*l
A|
*l
*l
1
1
Scorer
MP|
MP|
MP|
HP|
MP|
MP|
MP|
MP|
MP|
MP|
1
MP|
MP|
MP|
HP|
MP|
HP)
MP|
HP|
HP|
HP|
1
MP|
HP|
HP|
*P|
MP|
NP|
MP|
HP|
MP|
HP|
1
HP|
MP|
HP|
"I
HP|
MP|
MP|
MP|
HP|
MP|
1
MP|
MP|
MP|
MP|
HP,
MP|
MP|
MP|
MP|
«P|
1
1
Treat. Sex
Code
t|
t
t|
t|
t
t|
t|
t|
t
t
e|
c|
c
c|
c
tl
tl
tl
tl
tl
1
t
t
tl
tl
tl
tl
tl
tl
tl
tl
c
e
c
c
e
t
t|
t|
t|
t
1
t|
t
t
t|
t|
t|
t|
t|
t|
t
"1
"I
•1
H
•1
•1
•H
•1
m|
•1
1
'1
'1
'1
«l
*l
*l
'1
f|
'I
*l
1
*l
*l
*l
'1
-------
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
93
99
100
chr-8l
0|
0|
i
I
cells w/
5 CA
0|
«l
"I
0|
0|
o|
0|
»l
o|
o|
I
0|
°l
o|
o|
o|
o|
0|
»l
«l
«l
I
o|
0|
o|
0|
o|
o|
o|
0|
0|
o|
I
°l
"I
0|
»l
0|
«l
"I
0|
o|
o|
I
o|
o|
o|
0|
0|
6i
0|
«l
°l
0|
I
I
-------
cell* w/ cells w/ c«lU w/ cell* w/ cell* u/ pet. CA
6 CA 7 CA 8 CA 9 CA 10+ CA positive #
51 0|
52 OJ
53 OJ
54 0|
55 OJ
56 0|
57 OJ
58 0|
59 OJ
60 0|
1
1
61 0|
62 0|
63 OJ
64 OJ
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
o|
o|
o|
»l
o|
o|
1
1
0|
«l
o|
0|
0|
o|
»l
°l
0|
o|
1
1
0|
0|
o|
°l
o|
0|
o|
o|
0|
0|
1
0|
0|
"1
0|
0|
°l
°l
0|
l
°l
0|
0|
«l
0|
0|
01
1
0|
o|
0|
o|
0|
0|
0|
0|
o|
o|
1
1
1
-------
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
Animal
1154|
1155J
1156J
1157J
1158 j
1159J
1160)
1161)
1162J
1163 j
1
1
1164|
1165 1
1166 j
1167 j
1168 j
1169 1
1170J
1171)
1172|
1173|
1
1174|
1175|
1176|
1177|
1178|
1179|
1180J
1181 j
1182)
1183 j
I
1054 1
1055 1
1056)
1057 j
1058 j
1059]
1060J
1041 1
1062J
1063 j
I
1064 1
1065 1
1066 1
1067 1
1068 1
1069 1
1070|
1071 1
1072 1
1073 1
I
I
Slide
A|
A|
A|
A|
A|
*l
A|
A|
A|
A|
1
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
At
A|
A|
A|
A|
A
A
A
• B
B
B
B
B|
8|
8|
B|
3|
B|
1
B
B
B
B|
B|
B|
B|
B|
5
B|
Scorer
MP|
MP|
MP|
MP|
MP|
MP|
HP|
MP|
MP|
HP|
|
HP|
MP|
MP|
HP|
HP|
HP|
MP|
MP|
NP|
HP|
1
HP|
NP|
MP|
MP|
HP|
MP|
HP|
HP|
HP|
MP|
1
BN|
BN|
BN|
BN|
BN|
BN|
BN|
8M|
BK|
BN|
1
BN|
B*|
BN|
BN|
BM|
BN|
BN|
BN|
BN[
BM|
1
1
Treat. Sex
Code
c
c
c
c
c
t
tl
tl
tl
tl
1
1
tl
tl
tl
tl
tl
tl
tl
tl
tl
tl
1
Pi
Pi
Pi
Pi
Pi
Pi
p
Pi
Pi
Pi
1
c|
c|
c|
c|
c
t
t
tl
t
t
tl
tl
tl
tl
tl
tl
tl
tl
-------
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
chr-some
break
0|
0|
0|
0|
"I
"I
0|
0|
°l
0|
1
0|
0|
0|
0|
0|
0|
o|
0|
1|
o|
1
1|
0|
o|
11
°l
o|
Ol
o|
o|
11
1
0|
0|
0|
o|
01
0|
o|
o|
o|
0|
1
o|
0|
o|
0|
0|
11
o|
0|
i|
o|
I
I
ehr-tid
exchange
0|
0|
o|
0|
o|
0|
0|
0|
0|
0|
I
o|
o|
0|
o|
o|
0|
0|
0|
0|
o|
I
0|
0|
0|
0|
0|
11
*l
0|
2|
0|
1
0|
oi
o|
0|
0|
0|
0|
0|
o|
0|
1
0|
0|
0|
0|
0|
11
o|
o|
o|
o|
1
1
chr-some
exchange
o|
o|
01
0|
0|
0|
0|
o|
o|
o|
i
o|
0|
0|
o|
01
o|
o|
o|
0|
0|
1
0|
"1
0|
0|
0|
0|
0|
0|
o|
o|
1
0|
0|
0|
o|
0|
0|
0|
0|
0|
o|
1
0|
0|
0|
o|
0|
0|
0|
0|
0|
o|
1
1
other
aberratn
0|
»l
0|
0|
0|
0|
0|
0|
0|
0|
1
0|
01
o|
0|
0|
o|
o|
0|
o|
o|
1
0|
0|
0|
o|
0|
0|
0|
o|
0|
o|
1
0|
0|
0|
0|
0|
0|
0|
0|
0|
0|
1
0|
o!
0|
0|
o|
0|
0|
0|
o|
0|
1
1
CA/cell
exc gap
0.040)
0|
0|
0.040J
0|
0.040|
0.040J
0|
0.080|
0.080)
1
0.120|
0.080 j
0.040J
0.080 j
0.040 |
0.200|
0.040 |
0.120 j
0.080)
0.200 |
I
0.320 |
0.240)
0.160)
0.440 j
0.400 |
0.400 |
0.280 |
0.160)
0.240)
0.280)
1
0|
o|
0.800)
0.080 j
o|
0.040)
0.040)
o|
0.080)
0.080)
1
0.080)
0.080)
0|
0.160)
0.080 |
o.;?o|
0.120)
0.080)
0.200!
0.030)
I
I
cells w/
0 CA
24)
25)
25)
24)
25)
24)
24)
25)
23)
23)
I
22)
23)
24)
23)
24)
20 1
24)
22)
23)
20)
I
"I
19)
21)
15)
17)
16)
18)
21 1
19|
19)
1
25)
25)
5)
23|
25 1
24)
2*1
25)
23)
23)
1
23)
23|
25)
21)
23)
19)
22)
23)
20 1
23|
I
I
cells w/
1 CA
1|
o|
o|
11
0|
1)
1)
0|
2|
2|
I
3)
2|
M
2|
1|
5|
1|
3|
2)
5)
I
•I
«l
*l
'I
n
•I
7|
*l
«l
5|
I
0|
0|
20)
2)
0|
1|
'I
o|
2|
2|
1
2|
21
0|
*l
2|
5|
3)
2)
5|
2|
1
1
cells u/
2 CA
o|
o|
0|
o|
' o|
o|
0|
0|
0|
o|
1
0|
o|
0|
0|
0|
0|
0|
0|
0|
0|
1
0|
0)
"1
M
0|
11
0|
0|
0|
i|
1
o|
0|
o|
0|
0|
0|
01
o|
*l
0|
1
0|
0|
0|
0|
0|
M
0|
o|
0|
o|
I
I
cells M/
3 CA
0|
0)
0|
0|
o|
o|
0|
0|
0|
"I
I
o|
o|
0|
0|
0|
o|
0|
0|
0|
o|
I
0|
0|
0|
o|
1)
o|
o|
0|
0|
0|
I
o|
0|
o|
o|
0|
0|
0|
0|
o|
»l
I
0|
0|
0|
0|
0|
o|
0|
o|
0|
0|
I
I
cells M/
4 CA
0|
0|
0|
0)
0|
0|
o|
0|
0)
0|
I
0|
0|
"I
0|
0|
0|
0)
0|
0|
o|
I
0|
0|
o|
0|
0|
0|
0|
o|
0|
o|
I
0|
o|
0|
0|
o|
0|
0|
0|
o|
0|
I
0|
0|
o|
o|
0|
o|
o|
0|
0|
0|
I
I
cells w/
5 CA
0|
o|
0|
0|
0|
0|
0|
0|
0|
0|
I
0|
o|
0|
0|
0|
0|
0|
0|
0|
0|
I
0|
0|
0|
0|
"I
0|
0|
0|
0|
0|
I
0|
0|
0|
o|
0|
0|
0|
0|
0|
0|
I
o|
0|
0|
0|
ol
c|
0|
0|
0|
0|
I
I
11
-------
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
•i6
U7
148
149
150
celts w/
6 CA
°l
"I
o|
o|
°l
°l
0|
0|
o|
o|
I
o|
"I
o|
o|
o|
°l
0|
0|
0|
0|
I
o|
o|
o|
°l
o|
o|
°l
"I
o|
0|
I
0|
o|
0|
°l
o|
°l
o|
o|
"I
o|
I
°l
0|
o|
°l
°l
°l
°l
°l
0|
°l
I
I
cells u/
7 CA
0|
0|
o|
0|
0|
0|
o|
o|
o|
o|
I
o|
0|
"I
°l
0|
0|
°l
0|
0|
°l
I
o|
°l
o|
0|
o|
°l
0|
°l
o|
°l
I
o|
°l
0|
o|
0|
0|
o|
°l
o|
°l
I
o|
0|
°l
°l
°l
°l
o|
o|
o|
o|
I
I
cells w/
8 CA
o|
°l
0|
o|
0|
o|
°l
o|
o|
°l
I
o|
0|
o|
°l
°l
o|
°l
o|
°l
o|
I
o|
o|
°l
0|
0|
°l
o|
o|
o|
°l
I
°l
°l
°l
o|
°l
0|
o|
o|
o|
0|
I
o|
o|
°l
°l
o|
°l
o|
0|
°l
0|
I
I
cells w/
9 CA
0|
"I
"I
o|
0|
0|
°l
0|
«l
0|
I
°l
o|
0|
0|
o|
0|
0|
o|
o|
o|
I
o|
o|
"I
o|
o|
o|
"I
°l
"I
o|
I
o|
o|
0|
o|
o|
o|
o|
o|
°l
0|
I
0|
0|
o|
°l
°l
o|
o|
0|
o|
0|
I
I
cells w/
10+ CA
01
°l
f|
0|
0|
o|
o|
o|
o|
0|
0|
°l
0|
o|
0|
0|
o|
0|
o|
"1
1
o|
0|
0|
°l
o|
°l
o|
o|
o|
o|
1
°l
0|
0|
0|
0|
o|
o|
°l
°l
01
o|
o|
o|
°l
o|
0|
01
o|
o|
01
pet. CA
positive
4.0|
0.0|
0.0|
4.0|
0.0|
4.0|
4.0|
0.0|
s.oj
8.0J
1
12.0|
8.0J
4.0|
8.0|
4.0|
20. 0|
4.0|
12. 0|
8.0J
20. OJ
I
32. 0|
24. 0|
16. OJ
40.0)
32. OJ
36. 0|
28. OJ
16. OJ
24. OJ
24. OJ
I
0.0|
o.oj
80. OJ
8.0J
o.oj
4.0J
4.0|
0.0|
8.0|
8.0|
I
8.0|
8.0|
0.0|
16. OJ
8.0|
24. 0|
12. 0|
8.0|
20. OJ
8.0J
I
I
MI
# cells
500 1
500 1
500 j
500 j
500 j
500 j
500 j
500 1
500 1
500 j
I
500)
500 j
500 j
500 j
500 1
500 1
500 j
500 j
500 j
500 1
I
500 1
500 j
500 j
500 1
500 j
500 j
500 1
500 j
500 j
500 j
I
500 1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 1
500 j
500 1
500 1
500 1
500 1
500 j
500 1
500 1
I
I
MI
# meta
12|
15|
14|
25 1
24 1
21 1
25 1
15|
16|
25 1
I
28|
24 1
25 1
26|
28 1
24 1
25 1
26|
32 1
19|
I
15|
32 1
15|
28|
24 1
23|
25 1
25 1
21 1
25 1
1
25|
24 1
32 1
33 1
15|
15|
25 1
24 1
26 1
23|
I
25 1
14|
25 1
23|
25 1
32 1
18|
17|
16|
15|
1
1
MI
percent
2.40|
3.00|
2.80J
5.00J
4.80J
4.20J
5.00J
3. 00 j
3.20J
5.00J
1
5.60|
4.80J
s.ooj
5.20J
5. 60 j
4. 80 j
5.00J
5.20J
6.40J
3. 80 j
1
3.00|
6.40J
3.00J
S.60J
4.80J
4.60J
s.ooj
5.00J
4.20J
5.00J
1
5.00)
4.80J
6. 40 j
6.60J
3. 00 j
3. 00 j
s.ooj
4.80|
5.20J
4.60J
1
s.ooj
2.80J
5.00J
4.60J
S.00|
6.40|
3.60J
3.40|
3.20J
3.00|
1
1
12
-------
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
Animal Slide
1074 1
1075 1
1076 j
1077|
1078 1
1079J
1080 1
1081 1
1082 j
1083 1
I
1084)
1085 1
1086 j
1087 1
1088 j
1089 j
1090 1
1091 1
1092)
1093 j
I
1094 1
1095J
1096 1
1097J
1098 1
1099J
1100 1
1101 j
1102|
1103|
1
1104|
1105|
1106|
1107)
1108|
1109|
1110J
1111J
1112|
1113|
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1114)
1115J
1116|
1117)
1118J
1119|
1120)
1121 j
1122J
1123)
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B|
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Bl
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BN|
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Treat. Sex
Code
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500 j
500 j
500 j
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500 1
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1000 1
1000 j
1000 j
1000 1
1000|
2000 j
2000 j
2000 j
2000 1
2000 1
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500 1
500 1
500 j
500 j
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1000 j
1000 j
1000 1
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2000)
2000 j
2000 1
2000 1
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0|
o|
o|
°l
0|
500 1
500 1
500 1
500 1
500 1
I
I
Sample
Time
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
I
48|
48|
48 1
48 1
48 1
48 1
48|
48 1
48 1
48|
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72|
72J
72|
72|
72|
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72 1
72 1
72 1
72 1
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72 1
72 1
72 1
72 1
72 1
72 1
72 1
72 1
72 1
72 1
I
24 1
24 1
24 1
2* I
24 1
24 1
24 1
24 1
24 1
24 1
I
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Nutfcer
Cells
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
2S|
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25|
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
I
chr-tid
gap
0|
o|
0|
1|
"I
"I
o|
0|
3|
o|
I
"I
4|
0 1
Q 1
3|
Q 1
3 j
0|
°l
4I
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°l
o|
°l
°l
3|
oj
0|
o|
0|
°l
I
0|
°l
0|
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2|
o|
o|
°l
°l
0|
I
2|
0|
Q I
0 I
0 1
o|
Q I
Q I
0 1
3|
I
I
chr-some chr-tid
gap break
0| 0
0| 0
0| 1
0| 0
OJ 0
0| 3
0| 4
0| 5
0| 0
0| 2
1
0| 4
0| 4
0| 8
0| 3
0| 0
0| 8
0| 2
0| 7
0| 3
0| 3
1
0| 0
0| 0
0| 0
0| 1
0| 0
0| 1
0| 1
0| 2
0| 0
OJ 2
I
0| 2
0| 3
0| 4
0| 0
0| 2
0| 1
0| 3
0| 8
0| 5
0| 5
I
0| 0
0| 0
0 1 1
0 1 1
0| 1
0| 0
0| 0
0| 1
0| 2
0| 1
1
1
13
-------
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
1S9
190
191
192
193
194
195
196
197
198
199
200
chr-some
break
"I
o|
0|
0|
0|
0|
0|
o|
o|
o|
I
0|
1|
1|
0|
°l
1|
0|
0|
o|
°l
1
o|
o|
o|
"I
0|
o|
°l
o|
0|
0|
1
0|
o|
2|
"1
0|
"1
0|
o|
1|
°l
I
o|
»l
°l
0|
0|
0|
0|
o|
°l
0|
I
I
chr-tid
exchange
"I
0|
°l
o|
o|
0|
o|
11
0|
0|
I
"I
«l
11
o|
3|
0|
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1|
3|
o|
1
o|
o|
°l
o|
o|
°l
0|
°l
0|
0|
1
o|
o|
11
o|
o|
0|
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°l
0|
1
0|
0|
0|
o|
0|
°l
0|
0|
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0|
1
1
chr-some
exchange
0|
o|
o|
o|
0|
o|
0|
°l
0|
o|
1
o|
o|
o|
o|
o|
0|
0|
°l
o|
0|
1
0|
°l
0|
°l
0|
o|
0|
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°l
0|
1
0|
0|
0|
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0|
0|
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°l
o|
1
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0|
0|
0|
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°l
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°l
0|
0|
1
1
other
aberratn
o|
0|
o|
o|
"I
o|
°l
"1
0|
o|
1
0|
o|
o|
o|
0|
o|
o|
"1
o|
o|
1
0|
o|
°l
o|
o|
o|
°l
o|
0|
o|
1
«l
o|
0|
°l
o|
o|
0|
°l
°l
°l
1
"I
°l
0|
0|
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0|
°l
3|
°l
1
1
CA/cell
exc gap
°l
°l
0.040J
°l
0|
0.120J
0.160 |
0.240 j
o|
0.080)
I
0.160]
0.200 1
0.400 j
0.120 j
0.120 j
0.360 j
0.080 |
0.320 j
0.240J
0.120 j
I
0|
°l
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0.040)
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0.040 |
0.040 j
0.080 j
°l
o.osoj
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0.080)
0.120 j
0.280]
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0.080]
0.040]
0.120]
0.320 1
0.240]
0.200 |
I
°l
0|
0.040]
0.040]
0.040]
0|
0|
0.040]
0.080]
0.040|
I
I
cells w/
0 CA
25
25
24 1
25
25 1
22 1
21 1
19|
25 1
23
21 1
20 1
17|
22|
22 1
16]
23 1
18|
21 1
22 1
I
» I
25 1
25 1
24 1
25 1
24 1
24 1
23 1
25 1
23|
I
23 1
22|
19|
25 1
23 1
24 1
22 1
18|
21
20 1
25 1
25
24 1
24 1
24 1
25 1
25
24 1
23 1
24 1
cells u/
1 CA
°l
"I
1|
o|
"I
3|
*l
*l
°l
2|
*l
*l
n
3|
3|
'I
2|
«l
3|
3|
I
0|
°l
o|
11
0|
11
11
2|
"I
2|
I
2|
3|
5|
"I
2|
1|
3|
«l
2|
S|
0|
0|
1|
1
1|
°l
0|
11
2|
1|
cells u/
2 CA
o|
0|
0|
0|
"I
0|
o|
"I
o|
0|
I
0|
1|
0|
0|
o|
o|
o|
11
o|
o|
I
o|
0|
o|
0|
o|
"I
"I
"I
0|
0|
I
°l
0|
11
0|
o|
o|
o|
11
2|
0|
1
o|
0|
01
»l
°l
"I
0|
0|
0|
°l
1
1
cells w/
3 CA
0|
0|
0|
0|
0|
0|
0|
»l
o|
0|
1
o|
o|
11
0|
0|
0|
0|
"I
11
o|
1
0|
o|
0|
«l
o|
°l
»l
o|
"1
o|
1
°l
0|
o|
o|
o|
o|
o|
0|
o|
0|
1
°l
01
°l
0|
o|
0|
0|
0|
»l
°l
1
1
cells u/
4 CA
°l
o|
»l
0|
0|
o|
0|
o|
0|
o|
1
»l
0|
0|
o|
»l
o|
o|
"1
o|
«l
1
0|
o|
«l
0|
o|
0|
0|
"I
0|
0|
1
°l
0|
o|
0|
»l
°l
0|
0|
0|
o|
1
°l
0|
o|
o|
0|
o|
°l
0|
o|
0|
1
1
cells w/
5 CA
o|
o|
0|
0|
o|
0|
»l
»l
0|
0|
1
»l
0|
0|
o|
"I
0|
»l
o|
o|
o|
1
0|
0|
0|
o|
"1
"I
0|
0|
"I
o|
1
0|
0|
o|
o|
0|
«l
o|
o|
0|
0|
1
o|
0|
0|
o|
0|
0|
0|
0|
o|
0|
1
1
14
-------
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
cells w/
6 CA
o|
0|
o|
o|
o|
o|
o|
0|
o|
0|
1
0(
o|
0|
0|
0|
o|
o|
o|
o|
0|
1
0|
o|
0|
o|
0|
o|
0|
o|
0|
o|
1
0|
0|
0 1
Q j
Q 1
0|
o|
o|
Q I
Q 1
1
Q 1
Q I
31
0|
01
"I
0|
o|
0|
"I
1
1
cells w/
7 CA
o|
o|
o|
o|
o|
o|
o|
o|
0|
0|
1
0|
o|
o|
0|
0|
o|
Q 1
Q 1
0|
o|
1
0|
o|
o|
0|
"I
o|
o|
0|
o|
o|
1
0|
0|
o|
o|
o|
°l
Q 1
Q 1
Q 1
Q I
1
"I
0|
°l
0|
o|
01
0|
o|
o|
0|
1
1
cells w/
8 CA
0|
o|
o|
°l
o|
°l
°l
0|
0|
0|
1
0|
o|
0|
o|
o|
o|
°l
o|
0|
o|
1
°l
°l
o|
0|
°l
0|
0|
o|
0|
o|
1
°l
o|
"1
of
0|
o|
0 |
Q 1
o|
0|
1
0|
01
0|
o|
o|
o|
o|
0|
0|
0|
1
1
cells w/
9 CA
o|
o|
o|
Q |
Q 1
°l
o|
°l
0|
o|
1
0 |
Q I
o|
01
"I
o|
°l
0|
0|
o|
1
0|
°l
0|
o|
0|
o|
o|
0|
"I
0|
1
01
Q |
Q 1
o|
0|
o|
o|
"I
o|
0|
1
o|
o|
3|
°l
o|
0|
0|
0|
0|
0|
1
1
cells u/
10+ CA
o|
o|
0|
0|
0|
o|
o|
0|
0|
0|
I
°l
o|
0 I
Q 1
0|
o|
°l
o|
0|
o|
I
o|
o|
0|
°l
o|
0|
°l
°l
o|
0|
I
o|
°l
0|
o|
o|
0|
°l
o|
0|
Jl
I
°l
f|
°l
0|
o|
°l
o|
o|
o|
0|
I
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pet. CA
positive
0.0|
o.oj
4.0|
0.0|
0.0|
12. OJ
16. OJ
24. OJ
o.oj
8.0 j
I
16. 0|
20. OJ
32. OJ
12. OJ
12. OJ
36. OJ
8.0|
28. OJ
16. OJ
12. OJ
1
0.0|
o.oj
o.oj
4.0|
0.0|
4.0|
4.0|
8.0|
o.oj
8.0J
1
8.0|
12. OJ
24. OJ
o.oj
8.0J
4.0|
12. 0|
28. OJ
16. 0|
20. OJ
1
0.0|
0.0|
4.0|
4.0|
4.0|
0.0|
0.0|
4.0|
8.0|
4.0|
1
1
HI
# cells
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
1
500)
500 j
500 1
500 j
500 j
500 j
500 j
sooj
500 j
500 j
1
500 1
500J
sooj
sooj
sooj
sooj
sooj
sooj
sooj
sooj
1
500 1
sooj
sooj
sooj
500 1
sooj
sooj
sooj
sooj
sooj
1
500)
500 1
500 1
500 1
sooj
500 1
500 1
500 1
500 1
500 1
1
1
HI
K meta
25 1
35 1
32 1
24 1
26 1
15|
18|
17|
16|
14|
I
12|
10)
15|
18|
10|
9|
5|
2|
1|
8|
I
25 1
26 1
24 1
25 1
28 1
32 1
15|
24 1
25 1
15|
I
25 1
15|
1*1
15)
12|
11|
12I
13 1
10|
2|
I
25 1
32 1
25 1
23|
21 1
2M
25 1
25 1
25 1
21I
1
1
HI
percent
S.00|
7.00J
6.40J
4.80J
S.20J
3.00J
3.60J
3.40J
3.20J
2. 80 j
1
2.40[
2.00J
3.00J
3.60J
2.00J
1.80J
i.ooj
0.40J
0.20J
1.60|
1
s.ooj
S.20J
4.80J
s.ooj
S.60J
6.40J
3.00J
4.80|
s.ooj
3.00J
1
5.00|
3. 00 j
2. 80 j
3. 00 j
2.40J
2.20J
2.40J
2.60J
2.00J
0.40J
1
S.OOj
6.40J
S.OOj
4.60|
4.20J
4.80|
S.00|
S.OOj
s.ooj
4.20|
1
1
15
-------
Animal Slide
Scorer Treat.
Code
Sex
Dose
Sample Nunber chr-tid ehr-some chr-tid
Time
Cells
break
201
202
203
204
205
206
207
208
209
210
211
212
213
2U
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
1124|
1125J
1126J
1127J
1128 j
1129J
1130J
1131 1
1132 1
1133 1
1
1134|
1135 1
1136 1
1137J
1133 1
1139J
1140J
1141J
1142|
1143|
1
1144|
1145|
1146|
1H7J
1148 1
1149 1
1150)
1151|
1152|
1153|
1
1154|
1155|
1156 j
1157J
1158 1
1159|
1160 1
1161 1
1162 1
1163 1
I
1164 1
1165 1
1166 1
1167)
1168 1
1169)
1170)
1171 1
1172)
1173|
1
B
B|
B|
B
•1
•1
B|
B
B
B
B
•1
B
•1
•1
B
•1
B
B
B
B
B
B|
•1
•1
•1
•1
•1
B
B|
B|
B
B
B
B
B
•1
•1
•1
B|
•1
s|
B
B|
B
B|
B|
•1
•1
•1
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
BN|
BN|
1
BN|
BN|
8N|
BN|
BNJ
BN|
BN|
BN|
8N|
BN|
1
BM|
BM|
BH|
BN|
BN|
BN|
BN|
BM|
BN|
BN|
1
BN|
BN|
BN|
BN|
BN|
BN|
BN|
BN|
BN|
BN|
1
BN|
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
BN|
1
1
t
t
t
t
t
t
t
t
t
t
c
c
c
c
c
t
t
t
t
t
t
t
t
t
t
t
t
t
t
t
c
c
c
c
c
t
t
t
t
t
t
t
*
t
t
t
t
t
t
t
1 '1
1 '1
*l
1 fl
1 fl
1 '1
1
-------
201
202
203
204
205
206
207
206
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
chr-soM
break
0|
"I
0|
o|
o|
11
o|
°l
»l
o|
I
0|
o|
0|
°l
«l
°l
o|
o|
o|
°l
I
II
o|
0|
0|
11
11
0|
0|
o|
11
I
o|
°l
°l
o|
0|
°l
0|
Jl
o|
o|
I
11
0|
0|
°l
0|
11
0|
°l
o|
0|
I
I
chr-tid
exchange
o|
0|
°l
0|
°l
»l
"I
0|
0|
11
I
°l
0|
0|
o|
«l
°l
0|
o|
o|
o|
I
o|
"I
o|
0|
11
0|
0|
o|
o|
0|
I
0|
o|
0|
0|
°l
«l
o|
0|
o|
o|
I
0|
0|
0|
0|
o|
o|
o|
0|
"I
2|
I
I
chr-some
exchange
0|
0|
0|
0|
0|
0|
0|
o|
°l
«l
I
0|
°l
°l
0|
"I
0|
° I
°l
o|
0|
I
o|
o|
0|
0|
0|
°l
o|
0|
o|
»l
I
o|
0|
o|
o|
°l
«l
o|
Jl
o|
o|
I
0|
»l
0|
°l
"I
0|
°l
°l
0|
0|
I
I
other
aberratn
0|
o|
0|
0|
o|
0|
»l
0|
0|
0|
I
0|
o|
°l
0|
o|
0|
o|
0|
o|
°l
I
0|
0|
»l
0|
o|
0|
0|
o|
0|
o|
I
o|
o|
°l
0|
0|
o|
°l
o|
o|
°l
I
0|
°l
°l
o|
o|
0|
0|
°l
o|
°l
I
I
CA/cell
exc gap
0.040)
0.040 j
0.080 |
0.120 |
0.040J
0.080)
0.120)
0.160 |
0.120J
0.080 j
I
0|
0|
"I
0|
o|
0.080J
0.040 j
°l
0.080)
0.040 |
I
o.osoj
0.040)
0.080)
0.120 |
0.120)
0.240)
0.160 |
0.200)
0|
0.240)
1
0)
o|
0|
0.080)
0|
0.040)
0.040)
0.040)
0.080)
°l
1
0.120)
0.120)
0.120)
0.040 |
o|
0.240)
0.120)
0.040)
0.160)
0.200|
I
I
celts w/
0 CA
24)
• 24)
23 1
22 1
24)
23)
22)
21 1
22)
23 1
1
25)
25)
25)
25)
25 1
23)
24)
25 1
23)
24 1
1
23|
24)
23)
22 1
22 1
19|
21 1
20)
25)
19)
I
25)
25 1
25 1
23)
25)
24)
24)
24|
23 1
25)
I
22)
22 1
22)
24)
25)
19)
22 1
24 1
21 1
20)
I
I
celts w/
1 CA
1|
1|
2)
3|
1|
2|
3|
«l
3|
2|
I
o|
°l
o|
0|
"I
2|
1|
°l
2|
1|
1
2)
M
2|
3|
3|
*l
«l
5)
°l
6|
I
0|
o|
0|
2|
0|
1|
1|
M
2)
0|
I
3)
3)
3)
1|
o|
6|
3)
1|
*l
5)
I
I
cells w/
2 CA
0|
0|
"I
o|
0|
o|
0|
0|
o|
o|
I
o|
0|
o|
0|
o|
0|
0|
"I
o|
o|
I
0|
o|
°l
0|
0)
0|
«l
o|
°l
0|
I
0|
0|
0|
o|
0|
o|
0|
0|
0|
0|
I
o|
0|
0|
0|
0|
°l
0|
0)
o|
o|
I
I
cells y/
3 CA
o|
0|
0|
0|
0|
0|
0)
o|
o|
0|
I
o|
0|
0|
0|
»l
0|
"I
0|
0|
0|
I
o|
o|
o|
0|
"I
0|
»l
"I
o|
»l
I
o|
"I
o|
0|
.o|
0|
o|
o|
0|
o|
I
°l
°l
0|
0|
°l
0)
0|
°l
o|
0|
I
I
cells w/
4 CA
°l
°l
°l
«l
0|
0|
0|
o|
0|
o|
I
o|
o|
o|
o|
0|
«l
o|
o|
0|
o|
I
0)
o|
o|
0|
0|
0|
"I
o|
0|
o|
o|
0|
o|
0|
0|
o|
0|
»l
"I
0|
0|
0|
o|
3|
0|
0|
0|
°l
0|
0|
cells u/
5 CA
o|
o|
o|
0|
o|
0|
o|
0|
o|
«l
I
"I
o|
0|
0|
0|
«l
0|
°l
0)
o|
I
o|
0|
0|
0|
0|
o|
0|
o|
0|
o|
I
0)
°l
0|
0|
0|
0|
0|
o|
"I
o|
I
0|
o|
o|
o|
o|
»l
o|
0|
0|
»l
I
I
17
-------
201
202
203
204
205
206
207
208
209
210
211
212
213
2U
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
cells w/
6 CA
°l
0|
°l
°l
0|
"I
°l
o|
0|
o|
I
"I
0|
o|
°l
0|
°l
0|
0|
°l
o|
I
0|
0|
0|
0|
°l
o|
°l
°l
o|
0|
I
o|
°l
o|
o|
o|
o|
o|
°l
°l
o|
I
o|
o|
°l
o|
°\
o|
o|
°l
o|
o|
I
I
cells w/
7 CA
0|
o|
o|
0|
0|
o|
0|
°l
o|
o|
I
o|
o|
0|
o|
0|
o|
0|
0|
o|
°l
I
°l
°l
0|
°l
°l
o|
o|
°l
0|
o|
I
o|
o|
°l
o|
o|
o|
o|
o|
o|
°l
I
o|
"I
°l
°l
°l
°l
o|
o|
o|
°l
I
I
cells w/
8 CA
°l
o|
o|
0|
o|
°l
o|
o|
"I
0|
I
°l
o|
0|
0|
0|
°l
o|
0|
o|
"I
I
0|
°l
o|
°l
°l
0|
°l
o|
0|
o|
I
°l
°l
0|
0|
o|
°l
«l
0|
°l
o|
I
"I
o|
0|
o|
o|
°l
°l
-------
Animal Slide Scorer Treat. Sex Dose Sample Number chr-tid chr-some chr-tid
Code Time Cells gap gap break
251
252
253
254
255
256
257
258
259
260
1174|
1175J
1176J
1177)
1178 1
1179J
1180 j
1181|
1182 j
1183 1
1
1
B|
B|
B|
B|
Bl
B|
B|
B|
B|
B|
1
1
BK|
BX|
BN|
BN|
BN|
BN|
BN|
BNJ
BN|
BN|
1
1
P|
P|
Pi
Pi
Pi
P|
P|
Pi
Pi
Pi
1
1
m| 12. 5|
mj 12. 5|
mj 12.5J
mj 12. 5|
m| 12. 5|
f| 12.5|
f| 12. 5|
fj 12. 5|
fj 12. 5|
fj 12. 5|
I I
I I
48 1
48 1
48 1
48|
48|
48 1
48|
48|
48|
48 1
I
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
I..
o|
3|
0|
1|
0|
o|
5|
o|
0|
4|
I
I
"I
o|
0|
o|
o|
0 1
Q 1
Q 1
o|
0|
I
I
'I
7|
5|
0|
4|
4 1
3|
n
4|
5|
I
I
19
-------
chr-some ehr-tid chr-some other CA/cell cells w/ cells u/ cells w/ cells w/ cells w/ cells w/
break exchange exchange aberratn exc gap 0 CA 1 CA 2 CA 3 CA 4 CA 5 CA
251 |
252 |
253 |
254 |
255 |
256 |
257 |
258 |
259 j
260 j
I
I
2|
3|
1|
o|
o|
1|
o|
°l
o|
o|
1
I
3|
1|
o|
0|
»l
o|
°l
o|
o|
°l
I
I
»l
0|
o|
0|
°l
0|
"I
o|
o|
0|
I
I
o|
o|
0|
0|
0|
0|
o|
"I
0|
°l
I
I
0.560|
0.440|
0.240 j
o|
0.160J
0.200J
0.120J
0.280 j
0.160 |
0.200 |
I
I
14|
15|
19|
25 1
21 1
20 1
22 1
18|
21 1
20 1
1
1
8|
'1
«l
0|
*l
5|
3|
n
*l
5|
1
1
3|
M
°l
0|
0|
0|
o|
o|
«l
o|
I
I
o|
0|
0|
»l
o|
°l
0|
"I
o|
o|
I
I
°l
0|
0|
o|
0|
«l
0|
o|
o|
o|
I
I
o|
0|
0|
o|
0|
o|
0|
"I
«l
o|
I
I
20
-------
cells u/ cells M/ cells u/ cells w/ cells w/ pet. CA
6 CA 7 CA 8 CA 9 CA 10+ CA positive #
25 1
252
253
254
255
256
257
258
259
260
01
0|
0|
o|
o|
0|
o|
o|
o|
o|
1
1
0|
0|
«l
°l
0|
«l
0|
o|
o|
0|
1
1
0|
0|
o|
o|
0|
»l
"1
0|
0|
0|
1
1
0|
0|
0|
0|
o|
0|
o|
0|
o|
0|
1
1
0|
0|
0|
0|
0|
o|
°l
o|
"1
0|
1
1
44.0)
40. OJ
24.0 1
0.0|
16. OJ
20. 0|
12. OJ
28.0 j
16. 0|
20. 0|
1
1
MI MI HI
cells # meta percent
500)
500 j
500 [
500 j
500)
500 1
500 1
500 j
500 j
500 j
1
L
ts|
25 1
32 1
32 1
25 1
24 1
26 1
28|
29 1
27|
1
1
3.00|
5.00|
6.40J
6.40|
5. 00 1
4.80|
5. 20 j
5. 60 j
5. 80 j
5.40J
1
1
21
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Pet. Mean Pet.
1 3 0.0 24.0 m 60.000 13.600
2 133 0.0 24.0 m 80.000 17.600
22
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 30.014 24 0.1843
Sex 8.226 12 0.7673
Time 30.904 8 0.0001
23
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time = 24.00 (Males) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
4
8
15
31
Cells
Scored
200
250
250
250
Percent
Aberrant
2.0000
3.2000
6.0000
12.4000
SEM
(for Obs)
1.0690
0.9978
1.4907
1.9276
Pairwise
Significance
0.2162
0.0180
0.0000
Time
P
alpha
48.00 (Males) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
3
20
36
47
Cells
Scored
250
250
250
250
Percent
Aberrant
1.2000
8.0000
14.4000
18.8000
SEM
(for Obs)
0.6110
2.3851
2.6800
2.9242
Pairwise
Significance
0.0001
0.0000
0.0000
24
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time = 72.00 (Males) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
2
9
24
39
Cells
Scored
250
250
. 250
250
Percent
Aberrant
0.8000
3.6000
9.6000
15.6000
SEN
(for Obs)
0.5333
0.9333
2.3247
2.8875
Pairwise
Significance
0.0164
0.0000
0.0000
*
*
*
Time = 24.00 (Females) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
3
7
13
25
Cells
Scored
250
250
250
250
Percent
Aberrant
1.2000
2.8000
5.2000
10.0000
SEM
(for Obs)
0.6110
0.8537
1.0414
1.2293
Pairwise
Significance
0.1007
0.0055
0.0000
*
*
25
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time
P
alpha
48.00 (Females) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
3
17
25
34
Cells
Scored
250
250
250
250
Percent
Aberrant
1.2000
6.8000
10.0000
13.6000
SEM
(for Obs)
0.8537
1.2000
1.4907
3.0521
Pairwise
Significance
0.0007
0.0000
0.0000
Time = 72.00 (Females) for 06788S
p = 0.000
alpha = 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
4
11
19
35
Cells
Scored
250
250
250
250
Percent
Aberrant
1.6000
4.4000
7.6000
14.0000
SEM
(for Obs)
0.8844
0.9333
1.3920
2.2509
Pairwise
Significance
0.0332
0.0007
0.0000
25
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Pet. Mean Pet.
1 3 0.0 24.0 m 60.000 13.600
2 133 0.0 24.0 m 80.000 17.600
27
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 30.014 24 0.1843
Sex 8.226 12 0.7673
Time 30.904 8 0.0001
28
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time = 24.00 (Both sexes) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
7
15
28
56
Cells
Scored
450
500
500
500
Percent
Aberrant
1.5556
3.0000
5.6000
11.2000
SEM
(for Obs)
0.5729
0.6407
0.8897
1.1462
Pairwise
Significance
0.0697
0.0005
0.0000
*
*
Time = 48.00 (Both sexes) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
6
37
61
81
Cells
Scored
500
500
500
500
Percent
SEM
Pairwise
Aberrant (for Obs) Significance
1.2000
7.4000
12.2000
16.2000
0.5109
1.3067
1.5755
2.1418
0.0000
0.0000
0.0000
29
-------
filename: C:\CA\CATEST01.ILS date: time:
Time = 72.00 (Both sexes) for 067889
p = 0.000
alpha = 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant Cells Percent SEM Pairwise
MG/KG Cells Scored Aberrant (for Obs) Significance
0.00 6 500 1.2000 0.5109
500.00 20 500 4.0000 0.6489 0.0027 *
1000.00 43 500 8.6000 1.3385 0.0000 *
2000.00 74 500 14.8000 1.7912 0.0000 *
30
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Pet. Mean Pet.
1 3 0.0 24.0 m 60.000 13.600
2 133 0.0 24.0 m 80.000 17.600
31
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time =
alpha
48.00 (Males)
0.050
for 067889
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
(with positive controls)
Aberrant Cells Percent SEM
MG/KG Cells Scored Aberrant (for Obs)
0.00 3 250 1.2000 0.6110
12.50 67 250 26.8000 4.3019
Pairwise
Significance
0.0000
Time = 48.00 (Females) for 067889
alpha = 0.050
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
(with positive controls)
Aberrant Cells Percent SEM
MG/KG Cells Scored Aberrant (for Obs)
0.00 3 250 1.2000 0.8537
12.50 56 250 22.4000 2.2470
Pairwise
Significance
0.0000
32
-------
filename: C:\CA\CATEST01.ILS date:
time:
Endpoint: Chromosomal aberration (total aberrations)
Variance inflation factor:
Alpha:
Factor
Scorer
Sex
Time
Deviance
27.057
4.281
21.975
df
24
12
8
1.000
0.050
0.3018
0.9778
0.0050
33
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time - 24.00 (Males) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 4
500.00 8
1000.00 15
2000.00 32
Cells Aberrat'ns
SEM
Pairwise
Scored
200
250
250
250
/ Cell
0.0200
0.0320
0.0600
0.1280
(for Obs)
0.0107
0.010
0.0149
0.0222
Significance
0.2193
0.0201
0.0000
*
*
Time = 48.00 (Males) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 3
500.00 20
1000.00 39
2000.00 51
Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell (for Obs) Significance
0.0120
0.0800
0.1560
0.2040
0.0061
0.0239
0.0340
0.0318
0.0002
0.0000
0.0000
34
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time
P
alpha
72.00 (Males) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 2
500.00 9
1000.00 25
2000.00 44
Cells Aberrat'ns
Scored
250
250
250
250
/ Cell
0.0080
0.0360
0.1000
0.1760
SEM
(for Obs)
0.0053
0.0093
0.0262
0.0359
Pairwise
Significance
0.0174
0.0000
0.0000
Time = 24.00 (Females) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 3
500.00 7
1000.00 13
2000.00 25
Cells Aberrat'ns
SEM
Scored
250
250
250
250
/ Cell
0.0120
0.0280
0.0520
0.1000
(for Obs)
0.0061
0.0085
0.0104
0.0123
Pairwise
Significance
0.1030
0.0062
0.0000
35
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time = 48.00 (Females) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 3
500.00 17
1000.00 25
2000.00 . 34
Cells Aberrat'ns
Scored
250
250
250
250
/ Cell
0.0120
0.0680
0.1000
0.1360
SEM
(for Obs)
0.0085
0.0120
0.0149
0.0305
Pairwise
Significance
0.0009
0.0000
0.0000
*
*
Time
P
alpha
72.00 (Females) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 4
500.00 11
1000.00 19
2000.00 35
Cells Aberrat'ns
SEM
Scored
250
250
250
250
/ Cell (for Obs)
0.0160
0.0440
0.0760
0.1400
0.0088
0.0093
0.0139
0.0225
Pairwise
Significance
0.0354
0.0009
0.0000
36
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Abs/Cell Av Abs/Cell
1 3 0.0 24.0 m 0.600 0.136
2 133 0.0 24.0 m 0.800 0.176
37
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 27.057 24 0.3018
Sex 4.281 12 0.9778
Time 21.975 8 0.0050
38
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time = 24.00 (Both sexes) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 7
500.00 15
1000.00 28
2000.00 57
Cells Aberrat'ns
SEM
Pairwise
Scored
450
500
500
500
/ Cell (for Obs) Significance
0.0156
0.0300
0.0560
0.1140
0.0057
0.0064
0.0089
0.0127
0.0720
0.0006
0.0000
Time = 48.00 (Both sexes) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 6
500.00 37
1000.00 64
2000.00 85
Cells Aberrat'ns
SEM
Pairwise
Scored
500
500
500
500
/ Cell
0.0120
0.0740
0.1280
0.1700
(for Obs)
0.0051
0.0131
0.0192
0.0228
Significance
0.0000
0.0000
0.0000
39
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Abs/Cell Av Abs/Cell
1 3 0.0 24.0 m 0.600 0.136
2 133 0.0 24.0 m 0.800 0.176
40
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time =
alpha
48.00 (Males)
0.050
for 067889
One tailed test (Poisson) for chromosomal aberration (total aberrations)
(with positive controls)
Total
MG/KG Aberrat'ns
0.00 3
12.50 74
Cells Aberrat'ns
Scored / Cell
250 0.0120
250 0.2960
SEM
(for Obs)
0.0061
0.0531
Pairwise
Significance
0.0000
Time =
alpha
48.00 (Females)
0.050
for 067889
One tailed test (Poisson) for chromosomal aberration (total aberrations)
(with positive controls)
Total
MG/KG Aberrat'ns
0.00 3
12.50 58
Cells Aberrat'ns SEM
Scored / Cell (for Obs)
250 0.0120 0.0085
250 0.2320 0.0259
Pairwise
Significance
0.0000
41
-------
filename: C:\CA\CATEST01.ILS date: time:
Time = 72.00 (Both sexes) for 067889
p = 0.000
alpha = 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total Cells Aberrat'ns SEM Pairwise
MG/KG Aberrat'ns Scored / Cell (for Obs) Significance
0.00 6 500 0.0120 0.0051
500.00 20 500 0.0400 0.0065 0.0030 *
1000.00 44 500 0.0880 0.0147 0.0000 *
2000.00 79 500 0.1580 0.0210 0.0000 *
42
-------
filename: C:\CA\CATEST01.ILS date: time:
Endpoint: Mitotic Index
Variance inflation factor: 1.341
Alpha: 0.050
Factor Deviance df p
Scorer 52.058 24 0.0008
Sex 206.145 24 0.0000
Time 136.872 32 0.0000
43
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time
24.00 (Males) for 067889
P
alpha
0.768
0.050
Likelihood ratio for
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
196
218
213
202
mitotic
Cells
Scored
5000
5000
5000
5000
index
Mitotic
Index (%)
3.9200
4.3600
4.2600
4.0400
SEM
(for Obs)
0.5531
0.3874
0.2876
0.3745
Pairwise
Significance
0.8298
0.7707
0.6045
Time
P
alpha
48.00 (Males) for 067889
0.000
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
264
179
140
64
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.2800
3.5800
2.8000
1.2800
SEM
(for Obs)
0.3492
0.1849
0.1789
0.1937
Pairwise
Significance
0.0002
0.0000
0.0000
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time
P
alpha
72.00 (Males) for 067889
0.000
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
230
206
152
76
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.6000
4.1200
3.0400
1.5200
SEM
(for Obs)
0.3876
0.3441
0.2596
0.2585
Pairwise
Significance
0.1551
0.0002
0.0000
Tine = 24.00 (Females) for 067889
P
alpha
0.697
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
256
241
236
227
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.1200
4.8200
4.7200
4.5400
SEM
(for Obs)
0.2313
0.1093
0.1794
0.3724
Pairwise
Significance
0.2756
0.2123
0.1214
45
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time
P
alpha
48.00 (Females) for 067889
0.572
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
253
239
232
219
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.0600
4.7800
4.6400
4.3800
SEM
(for Obs)
0.4927
0.3299
0.5504
0.4263
Pairwise
Significance
0.2881
0.1993
0.0831
Time = 72.00 (Females) for 067889
P
alpha
0.707
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
222
212
240
224
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.4400
4.2400
4.8000
4.4800
SEM
(for Obs)
0.4020
0.3180
0.1978
0.3593
Pairwise
Significance
0.3359
0.7705
0.5333
46
-------
filename: C:\CA\CATEST01.ILS date:
time:
Time = 48.00 (Males) for 067889
alpha = 0.050
Likelihood ratio for mitotic index (with positive controls)
Pair-wise
Significance
0.1692
MG/KG
0.00
12.50
Cells in
Mitosis
264
243
Cells
Scored
5000
5000
Mitotic
Index (%)
5.2800
4.8600
SEM
(for Obs)
0.3492
0.4483
Time = 48.00 (Females) for 067889
alpha = 0.050
Likelihood ratio for mitotic index
(with positive controls)
MG/KG
0.00
12.50
Cells in
Mitosis
253
253
Cells
Scored
5000
5000
Mitotic
Index (%)
5.0600
5.0600
SEM
(for Obs)
0.4927
0.1492
Pairwise
Significance
0.5000
-------
SAMPLE TEST DATA
CATEST02
-------
C:\CA\CATEST02.ILS listed at
on
EXPERIMENT
Record
Laboratory
Lab Book
Date Started ...
Date Completed .
Slides Coded By
Staining Method
Scored By
Entered By
Entry Date
Proofed By
Remarks
CA-TEST 02
121
ILS
III
06/18/90
08/01/90
MP
GIEMSA
CJH
MP
07/25/90
DC
TEST ARTICLE
Receipt Date ......
CAS #
Source / Lot
Appearance
Purity
Stability
Storage Conditions
Solubility
Hazard Information
Remarks
067889
04/20/90
UNKNOWN
RADIAN 067H3
ORANGE POWDER
UNKNOWN '
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER
TREAT AS CARCINOGEN
48
-------
VEHICLE ,
Source / Lot ,
Purity ,
Stability ,
Storage Conditions
CORN OIL
SIGMA/13F100
UNKNOWN
1 YEAR
ROOM TEMPERATURE
Remarks
POSITIVE CONTROL . .
Receipt Date
CAS #
Source / Lot
Appearance
Purity
Stability
Storage Conditions
Solubility
Hazard Information
Remarks
991870
01/22/90
UNKNOWN
RADIAN/110BD
WHITE POWDER
UNKNOWN
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER
CARCINOGEN
49
-------
TEST SYSTEM
Species
Strain
Supplier ...
Received ...
Quarantined
From
Until
MOUSE
B6C3F1
TACONIC FARMS
05/01/90
05/01
05/15
Routine Husbandry Conditions? (y/n) Y
Age
Sex Fr. To
M 11 11
F 11 11
Age units WEEKS
Weight
Fr. To
32.0 34.0
27.0 29.0
Weight units GRAM
Remarks
TREATMENT
Date Started
Date Completed ...
Route
Volume
Doses
Dose Units
Number Treatments
Treatment Duration
Treatment Date ...
Treatment Interval
Number Samples ...
Sample Date
Sample Interval ..
Tissue Cell Type
Remarks
06/18/90
06/21/90
IP
0.4
0, 500, 1000, 2000
MG/KG
1
NA
06/18/90
NA
3
06/19;06/20;06/21
24 HR
50
-------
Animal Slide Scorer Treat. Sex Dose Sample Number chr-tid chr-some chr-tid
Code Time Cells gap gap break
1
2
3
4
5
6
7
8
9
10
11
12
13
14
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23
24
25
26
27
28
29
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31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
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1055 j
1056 1
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1061 1
1062 1
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i
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o|
o|
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°l
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0|
0|
o|
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o|
oi
0|
0|
o|
0|
Q 1
Q 1
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I
0|
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Q I
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Q |
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I
2|
2|
15|
2|
2|
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0|
11
1
1
2|
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1|
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3|
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M
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M
M
I
I
51
-------
1
2
3
4
5
6
7
8
9
10
11
12
13
U
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
chr-some
break
0|
0|
o|
o|
o|
o|
o|
"I
o|
0|
I
0|
o|
°l
°l
°l
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0|
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0|
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0|
0|
o|
0|
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o|
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0|
I
°l
°l
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o|
o|
0|
o|
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o|
0|
I
I
chr-tid
exchange
o|
°l
o|
o|
o|
o|
o|
o|
o|
o|
I
°l
°l
°l
0|
°l
o|
o|
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o|
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I
o|
0|
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0|
0|
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0|
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0|
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chr-some
exchange
"I
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0|
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0|
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I
0|
o|
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°l
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I
0|
o|
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0|
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0|
0|
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°l
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other
aberratn
°l
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°l
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°l
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0|
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o|
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0|
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0|
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°l
°l
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0|
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0|
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0|
0|
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CA/cell
exc gap
0.080 |
0.080 j
0.600 |
0.080 j
0.080 |
o|
o|
o|
0.040]
0.040 j
I
0.040 |
o.oaoj
0.040 j
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0.040)
0.040 |
0.160 j
0.120 j
0.080 j
0.040 j
I
0.040J
0.120 j
0.080 j
0.080J
0.040 |
0.040 |
0.080 |
o|
0.040|
0.040 |
I
0.040 |
0.080)
0.120 |
0.200J
o|
0.240|
0.120|
0.080 |
0.280 j
0.200 |
I
o.oeoj
o.oaoj
0.120|
0.040J
0.040|
0.040|
0|
0|
0.040|
0.040J
1
1
cells w/
0 CA
23 1
231
10|
23 1
23 1
25 1
25 1
25 1
24 1
24 1
I
24 1
24 1
24 1
25 1
24 1
24 1
24 1
22|
24 1
24 1
I
24 1
24 1
23 1
23 1
24 1
24 1
24 1
25 1
24 1
24 1
I
24 1
24 1
24 1
24 1
25 1
24 1
24 1
24 1
23 1
24 1
I
23 1
24 1
24 1
24 1
24 1
2* I
25 1
25 1
24 1
24 1
I
I
cells w/
1 CA
2|
2|
15|
2|
2|
o|
°l
0|
1|
1|
I
1|
o|
1|
0|
1|
1|
o|
0|
"1
'I
1
1|
o|
2|
2|
1|
1|
0|
o|
1|
1|
I
M
°l
°l
o|
0|
"I
f|
°l
o|
o|
I
2|
1|
o|
1|
1!
1|
o|
°l
1|
1|
I
I
cells w/
2 CA
°l
"I
"I
0|
°l
°l
°l
0|
o|
o|
I
0|
1|
o|
0|
o|
o|
0|
o|
1|
°l
1
0|
o|
o|
°l
o|
o|
M
o|
o|
o|
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"I
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o|
o|
o|
o|
II
11
"1
1
"1
°l
o|
o|
of
o|
°l
01
0|
o|
1
1
cells u/
3 CA
°l
°l
o|
o|
"I
o|
"1
o|
o|
01
1
°l
0|
0|
"I
o|
o|
0|
3|
0|
o|
1
o|
1|
0|
°l
0|
o|
0|
o|
o|
"I
I
o|
°l
11
0|
o|
o|
11
0|
o|
0|
I
0|
"I
II
0|
0|
0|
"1
°l
0|
0|
1
I
cells u/
4 CA
°l
0|
0|
o|
°l
0|
o|
o|
o|
o|
1
o|
"1
o|
0|
°l
0|
11
0|
0|
-------
1
2
3
4
5
6
7
8
9
10
11
12
13
U
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
cells w/
6 CA
0|
o|
0|
°l
o|
°l
o|
o|
°l
°l
1
o|
°l
o|
0|
o|
0|
o|
o|
0|
o|
1
0|
°l
0|
o|
o|
°l
°l
0|
0|
0|
1
°l
0|
0|
o|
0|
11
°l
°l
°l
o|
I
0|
o|
°l
0|
°l
0|
°l
o|
°l
°l
I
I
cells w/
7 CA
"I
o|
o|
°l
o|
o|
°l
°l
o|
o|
I
°l
°l
°l
°l
o|
o|
o|
°l
o|
°l
I
°l
o|
°l
o|
0|
°l
0|
o|
o|
°l
I
°l
°l
°l
°l
o|
o|
o|
°l
o|
o|
I
0|
o|
o|
°l
o|
°l
o|
"I
°l
°l
I
I
cells u/
8 CA
°l
0|
o|
°l
°l
"I
°l
0|
o|
°l
I
0|
0|
o|
"I
o|
°l
°l
o|
o|
°l
I
0|
0|
o|
o|
°l
°l
o|
°l
o|
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I
o|
o|
o|
o|
o|
"I
o|
o|
°l
o|
I
0|
o|
0|
°l
o|
0|
o|
o|
o|
o|
cells w/
9 CA
o|
o|
0|
"I
o|
°l
0|
o|
o|
"I
I
0|
0|
o|
o|
o|
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o|
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"I
0|
0|
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o|
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o|
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o|
o|
o|
I
°l
o|
0|
o|
°l
^l
°l
°l
o|
°l
I
I
cells u/
10+ CA
o|
°l
o|
o|
°l
o|
0|
0|
°l
o|
I
o|
o|
"I
o|
o|
0|
°l
0|
o|
"I
I
o|
0|
o|
o|
o|
0|
o|
o|
o|
o|
I
o|
o|
o|
o|
o|
°l
o|
0|
o|
o|
I
o|
0|
o^
'l
-------
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
00
Animal Slide
1104 1
1105 1
1106J
1107J
1108 1
1109J
1110J
1111J
1112J
1113J
I
1114|
1115J
1116J
1117J
1118J
1119J
1120 j
1121|
1122|
1123|
1
1124|
1125|
1126|
1127|
1128|
1129|
1130 1
1131 j
1132 1
1133 1
1
1134|
1135J
1136J
1137|
1138 j
1139 j
1140 j
1141J
1142|
1143J
I
1144J
1145J
1H6J
1147]
Vusj
1149|
1150|
1151 1
1152J
1153J
I
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
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A|
A|
I
I
Scorer
HP |
HP |
HP |
HP |
MP|
HP |
HP |
HP |
HP |
HP|
I
HP|
HP |
HP |
HP |
HP |
HP |
HP |
HP|
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP|
HP)
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
I
Treat.
Code
t
t
t
t
t
t
t
t
t
t
c
c
c
e
c
t
t
t
t
t
t
t
t
t
t
t
t
t
t
t
c
c
c
c
c
t
t
t
t
t
t
t
t
t
t
t
t
t
t
t
Sex
I H
I «l
I «l
I -I
I «l
I «l
I •!
•I
I H
I H
I I
I f|
I f|
I »l
I «l
I f|
I f|
I *l
'I
f|
I *l
I
'I
f|
'I
I
-------
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
chr-some
break
0|
o|
0|
0|
0|
0|
0|
°l
o|
o|
I
o|
o|
0|
o|
o|
o|
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0|
0|
°l
I
o|
o|
2|
o|
0|
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o|
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11
0|
I
°l
o|
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0|
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0|
o|
o|
o|
o|
I
11
0|
°l
11
°l
°l
o|
o|
°l
"I
1
1
ehr-tid
exchange
°l
°l
°l
°l
°l
o|
°l
°l
°l
0|
1
o|
°l
"1
o|
o|
o|
o|
0|
0|
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1
o|
o|
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1
o|
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0|
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o|
1
o|
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o|
o|
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°l
°l
°l
o|
1
1
chr-some
exchange
°l
°l
0|
"1
o|
°l
°l
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0|
°l
1
°l
o|
°l
°l
0|
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0|
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01
>l
°l
°l
1
1
other
aberratn
0|
0|
o|
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0|
°l
0|
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0|
1
°l
0|
0|
o|
0|
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0|
0|
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1
0|
0|
o|
o|
°l
°l
0|
0|
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1
0|
0|
0|
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0|
0|
0|
0|
0|
0|
1
0|
0|
o|
o|
°l
0|
0|
0|
0|
»l
1
1
CA/cell
exc gap
0.120|
0.040 |
°l
0.040]
0.120 j
0.240 |
0.080 j
0.040 j
0.120 j
0.040 j
I
0.040)
0.040 j
0.040J
0.040 j
0.040J
0.040 |
0.040|
«l
0.040|
o|
I
0.040|
0.040 j
0.160 j
0.040 |
0|
0.080|
0.080 j
0.040 j
0.160 j
0.120 j
1
0.040J
0.080 j
o|
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0.080)
0.080J
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1
0.200)
0.120)
o.owj
0.200)
0.040)
0.040 j
0.120)
0)
0.240)
0.120)
I
I
cells w/
0 CA
2* I
24)
25)
24)
24)
24 1
24)
24)
24)
24)
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24)
24)
24)
24)
24)
24)
24)
25)
24 1
25 1
I
24)
24)
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24)
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24)
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24)
24)
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24)
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25)
25)
24)
24)
24)
24)
24)
I
22)
24)
24)
24)
24)
24)
24 1
25 1
24)
24)
I
I
cells w/
1 CA
«l
11
"1
M
0|
o|
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1)
°l
11
I
I
I
I
I
I
11
11
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11
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1
11
11
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11
o|
0)
0|
11
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0|
11
2|
0|
0|
0)
11
0|
0|
o|
»l
1
11
o|
0|
°l
M
1)
0)
0|
0)
o|
I
I
cells w/
2 CA
°l
°l
"I
0|
°l
0|
11
0)
0)
0|
1
0|
0)
0|
o|
o|
0|
0|
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°l
o|
1
0|
°l
2|
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0|
11
11
°l
o|
11
1
o|
o|
°l
o|
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11
11
0|
0|
1
2|
0|
11
o|
0|
0)
o|
°l
o|
"I
1
1
cells w/
3 CA
1|
«l
"1
0|
1|
0|
o|
0)
M
o|
I
"I
0|
o|
»l
«l
o|
0|
o|
0|
o|
I
0|
o|
°l
0|
o|
0|
o|
0)
o|
0|
I
0|
0|
"I
0|
0|
0|
0|
o|
1)
1)
I
o|
11
0|
0|
0|
o|
11
0|
°l
1)
1
1
cells w/
4 CA
»l
0|
o|
0|
«l
0|
0|
°l
"1
°l
1
-------
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
cells w/
6 CA
0|
o|
0|
o|
o|
1|
o|
01
o|
"1
1
0|
0|
0|
o|
o|
0|
o|
0|
o|
0|
1
°l
o|
»l
o|
0|
°l
°l
0|
o|
0|
1
°i
0|
o|
"I
0|
0|
0|
0|
0|
o|
1
0|
°l
°l
0|
0|
0|
o|
o|
11
o|
I
I
cells w/
7 CA
o|
0|
0|
0|
o|
o|
0|
0|
o|
0|
I
0|
0|
0|
o|
o|
0|
0|
0|
0|
0|
I
o|
o|
0|
o|
o|
o|
0|
0|
0|
o|
I
°l
0|
o|
0|
0|
0|
o|
o|
«l
o|
I
o|
o|
0|
0|
o|
o|
0|
0|
0|
0|
I
I
cells w/
8 CA
0|
0|
0|
o|
o|
o|
o|
0|
0|
0|
I
0|
o|
o|
0|
0|
0|
o|
°l
«l
o|
I
o|
0|
0|
0|
«l
0|
o|
«l
°l
0|
I
o|
0|
0|
o|
o|
0|
0|
o|
°l
o|
I
°l
o!
"I
°l
0|
0|
0|
0|
°l
°l
I
I
cells u/
9 CA
o|
0|
°l
o|
0|
o|
«l
°l
o|
0|
I
0|
0|
0|
0|
°l
0|
o|
o|
°l
0|
I
0|
0|
°l
0|
o|
o|
0|
0|
«l
°l
I
o|
0|
0|
o|
o|
0|
0|
0|
0|
o|
I
0|
0|
°l
°l
0|
o|
0|
o|
»l
0|
cells M/
10+ CA
°l
0|
0|
0|
0|
0|
0|
°l
0|
o|
I
0|
0|
0|
o|
o|
0|
0|
o|
o|
0|
I
o|
0|
o|
0|
o|
t»l
0|
o|
»l
0|
I
°l
0|
o|
o|
0|
0|
o|
0|
"I
«l
I
o|
»l
0|
°l
0|
o|
0|
0|
o|
0|
I
I
pet. CA
positive
4.0|
4.0|
0.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
I
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
0.0|
4.0|
0.0|
I
4.0|
4.0|
8.0|
4.0|
0.0|
4.0|
4.0|
4.0|
4.0|
4.0|
I
4.0|
8.0|
o.oj
o.oj
o.oj
4.0|
4.0|
4.0|
4.0|
4.0|
I
12. 0|
4.0|
4.0|
4.0|
4.0|
A.0|
4.0|
0.0|
4.0|
4.0|
I
I
HI
# cells
500 1
500 1
500 1
500 j
500 j
500 j
500 j
500 1
500 1
500 j
I
500 1
500 j
500 j
500 j
500 j
500 1
500 1
500 j
500 j
500 1
I
500 1
500 1
500 j
500 j
500 j
500 1
500 j
500 j
500 1
500 j
I
500 1
500 1
500 1
500 j
500 j
500 j
500 1
500 1
500 1
500 1
I
500 1
500 1
500 1
500 1
500 1
SCO |
500 j
500 1
500 1
500 1
I
I
MI
# meta
«|
121
"1
14 1
19|
2|
5|
*l
•I
9|
I
25 1
24 1
23|
26|
32 1
25 1
24 1
26|
25 1
21 1
I
24 1
23|
23|
24 1
25 1
32 1
25 1
21 1
22 1
19|
I
25 1
18|
17|
25 1
32 1
31|
30 1
18|
19|
25 1
1
«|
18|
20 1
30 1
14|
18|
19|
25 1
14|
15|
1
1
HI
percent
3.00|
2.40J
2.20J
2.80|
3. 80 1
0.40 j
i.ooj
0.80J
1.60J
1.80 j
I
5.00|
4. 80 1
4.60J
5. 20 1
6.40J
5. 00 1
4. 80 1
5. 20 j
5.00J
4. 20 1
I
4.80J
4. 60 j
4.60J
4. 80 1
5.00J
6.40J
5. 00 j
4.20J
4. 40 j
3. 80 1
I
5. 00 1
3.60 j
3. 40 1
5. 00 1
6. 40 j
6.20J
6. 00 1
3. 60 1
3. 80 1
5.00|
I
3.00|
3. 60 1
4.00|
6.00|
2. 80 j
3. 60 1
3. 80 1
5. 00 j
2.80|
3. 00 1
I
I
56
-------
101
102
103
104
105
106
107
108
109
110
111
112
•113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
HO
141
142
143
144
145
K6
147
148
149
150
Animal Slide
1154)
1155J
11S6J
1157J
1158J
1159 j
1160J
1161 1
1162 j
1163 1
I
1164 1
1165J
1166 1
1167|
1168 1
1169 j
1170J
1171)
1172)
1173J
1
1174|
1175J
1176 j
1177J
1178|
1179|
1180|
1181|
1182|
1183|
1
1054|
1055 j
1056 j
1057J
1058 j
1059 1
1060 j
1061 j
1062 1
1063 j
I
1064 1
1065 j
1066J
1067|
1068J
1069 1
1070 1
1071 1
1072 1
1073J
I
I
A|
A
A
A
A
A
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A
A|
I
B|
B|
B|
B|
B|
8|
B|
B|
B|
B|
B
B
B
r,
B|
B|
B|
B|
B|
B
Scorer
MP|
HP |
MP|
MP|
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP|
HP |
HP |
I
HP |
""I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
BN|
BN|
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
I
BN|
3N|
BH|
BM|
BN|
BN|
BM|
BN|
BN|
BN|
I
I
Treat.
Code
c
c
c
c
c
t
t
t
t
t
t
t
t
t
t
t
t
t
t
t
P
P
P
P
P
P
P
P
P
P
c
c
c
c
c
t
t
t
t
t
t
t
t
I
t
I
t
t
t
t
Sex
I f
I '
I f
I f
I f
f
'
f
f
f
I f
I f
f
f
f
'
f
*
f
f
m
m
m
m
R.
f
f
f
f
'
ID
m
m
m
m
ID
m
m
m
m
m
m
01
fli
in
m
m
nt
m
m
Dose
1 o|
1 0|
1
-------
101
102
103
104
105
106
107
108
109
110
111
112
113
1U
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
chr-some
break
0|
o|
«l
°l
0|
0|
0|
0|
0|
o|
I
o|
°!
0|
°l
o|
0|
0|
"I
11
°l
1
11
o|
o|
11
0|
"I
0|
0|
°l
11
I
"I
o|
0|
"I
0|
»l
°l
o|
0|
0|
I
o|
0|
0|
0|
0|
1]
0|
o|
11
0|
I
I
ehr-tid
exchange
o|
°l
0|
o|
0|
o|
o|
o|
0|
o|
I
o|
o|
o|
0|
0|
o|
°l
0|
0|
0|
I
o|
o|
o|
11
0|
11
*l
o|
2|
°l
I
°l
°l
0|
o|
0|
0|
o|
o|
°l
0|
I
0|
0|
°l
°l
°l
11
0|
o|
o|
o|
1
1
chr-some
exchange
o|
0|
o|
"1
°l
o|
0|
°l
°l
"I
1
°l
"I
0|
°l
°l
0|
0|
°l
0|
0|
1
0|
0|
o|
0|
0|
0|
0|
o|
0|
0|
1
°l
o|
°l
o|
o|
o|
0|
o|
0|
°l
1
0|
0|
o|
o|
o|
0|
°l
0|
0|
o|
1
1
other
aberratn
"I
0|
0|
0|
0|
0|
o|
°l
°l
°l
1
0|
0|
°l
°l
0|
°l
0|
"I
o|
0|
1
0|
0|
0|
o|
0|
o|
o|
0|
o|
0|
1
o|
"1
0|
o|
0|
0|
"1
0|
o|
0|
1
0|
°l
• o|
°l
0|
0|
°l
°l
o|
0|
I
1
CA/cell
exc gap
0.040)
0|
0.120)
0.040 |
0|
0.040)
0.040)
°l
0.080)
0.080)
I
0.120J
0.080)
0.040)
0.080)
0.040)
0.200)
0.040)
0.120)
0.080)
0.200)
I
0.320 |
0.240)
0.160)
0.520)
0.400)
0.400 j
0.280 1
0.160)
0.240)
0.280)
I
0.080)
0.040)
0.800 j
0.080)
0|
0.040)
0.040)
0|
0.080)
0.080)
I
0.080)
0.080)
°l
0.160)
0.080)
0.280)
0.160)
0.080)
0.200)
0.080)
1
1
cells w/
0 CA
24)
25 1
22 1
24 1
25 1
24 1
24 1
25 1
24 1
24)
1
24)
24 1
24 1
24 1
24 1
24)
24)
24)
24)
24 1
1
24 1
24 1
24 1
22 1
23)
23 1
24)
24)
2*1
23 1
1
23)
24 1
5|
23 1
25)
24)
24 1
25 1
24)
24)
1
2*1
24)
25)
24)
24)
23 1
24)
24)
24)
24)
1
1
cells M/
1 CA
M
°l
3|
1|
0|
1|
M
o|
0|
0|
1
o|
o|
11
°l
11
o|
11
0|
0|
o|
I
o|
o|
»l
11
0|
o|
0)
0|
«l
0|
I
2|
M
20)
2|
0)
1|
1|
0|
o|
0|
I
0|
"I
0|
0|
o|
°l
o|
o|
0|
"I
I
I
cells w/
2 CA
°l
"I
0|
o|
"I
«l
o|
o|
1|
11
1
o|
M
0|
1|
»l
o|
o|
o|
11
o|
I
"I
o|
°l
11
o|
11
0|
0|
o|
11
I
0|
"I
0|
o|
0|
0|
o|
0)
11
11
I
11
11
0|
0|
1)
11
0|
11
3 1
11
1
1
cells u/
3 CA
°l
0|
0|
0|
0|
o|
0|
0|
0|
o|
1
1|
«l
"I
o|
o|
0|
o|
11
°l
"1
1
0|
o|
°l
o|
11
o|
o|
"I
"I
"I
I
°l
o|
o|
o|
o|
0|
°l
0|
0|
»l
I
°l
0|
0|
0|
0|
°l
f|
0|
o|
0|
I
I
cells w/
4 CA
o|
°l
0|
0|
o|
0|
o|
«l
«l
«l
I
0|
0|
o|
0|
o|
o|
0|
»l
0|
0|
I
0|
o|
M
°l
0|
0|
0|
11
0|
"I
1
°l
0|
0|
o|
°l
o|
o|
0|
o|
"1
1
°l
o|
0|
M
0|
0|
11
o|
"I
3 1
I
I
cells w/
5 CA
°l
"I
0)
o|
°l
0|
o|
o|
0|
0|
I
0|
»l
0|
0|
o|
11
0|
o|
°l
11
1
»l
0|
"1
0|
o|
0|
0|
0|
o|
11
1
o|
0|
"1
o|
»l
«l
0|
0)
o|
0|
1
o|
o|
0|
0|
0)
11
."I
0|
11
0|
I
I
58
-------
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
cells w/
6 CA
0|
"I
°l
0|
o|
°l
o|
o|
°l
o|
I
0|
o|
o|
0|
o|
0|
°l
0|
«l
0|
I
0|
11
o|
o|
0|
o|
o|
o|
11
0|
I
o|
o|
o|
0|
0|
o|
o|
o|
°l
0 1
I
o|
°l
0|
°l
o|
o|
°l
°l
o|
0|
I
I
cell* w/
7 CA
o|
°l
o|
o|
o|
o|
o|
0|
o|
o|
I
°l
o|
o|
°l
0|
o|
o|
°l
°l
o|
I
0|
°l
°l
o|
11
o|
11
°l
o|
o|
I
o|
o|
0|
o|
o|
°!
o|
o|
o|
o|
I
o|
°l
o|
o|
o|
o|
o|
"I
"I
o|
I
I
cells w/
8 CA
°l
"I
o|
o|
0|
o|
o|
o|
0|
o|
I
0|
0|
0|
o|
°l
°l
°l
°l
°l
"I
I
11
0|
0|
°l
°l
11
o|
°l
°l
°l
I
0|
o|
o|
o|
o|
°l
o|
°l
o|
o|
I
°l
°l
°l
°!
0|
°l
0|
0|
0|
0|
I
'
cells w/
9 CA
"I
0|
°l
0|
°l
0|
0|
0|
o|
o|
I
o|
"I
o|
o|
"I
o|
0|
»l
»1
o|
1
o|
0|
0|
o|
o|
0|
0|
o|
»l
o|
1
0|
o|
"1
°l
0|
0|
0|
0|
o|
0|
1
0|
0|
0|
0|
o|
0|
0|
0|
0|
"1
!
i
cells w/
10* CA
o|
0|
0|
0|
0|
o|
o|
0|
o|
"I
I
o|
«l
«l
0|
»l
0|
o|
o|
o|
o|
I
o|
o|
0|
11
o|
"1
0|
0|
0|
"1
1
"I
"I
o|
0|
0|
0|
0|
"1
0|
0|
1
o|
0|
0|
o|
0|
o|
o|
0|
0|
0|
1
1
pet. CA
positive
4.0|
0.0|
12. 0|
4.0|
o.oj
4.0|
4.0|
o.oj
4.0|
4.0|
I
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
I
4.0|
4.0|
4.0|
12. 0|
8.0J
8.0J
4.0|
4.0|
4.0|
8.0|
I
8.0|
4.0|
80. 0|
8.0J
0.0|
4.0|
4.0|
0.0|
4.0|
4.0|
I
4.0|
4.0|
0.0|
4.0|
4.0|
8.0|
4.0|
4.0|
4.0)
4.0|
I
I
MI
# cells
500 1
500 1
500 j
500 j
500 j
500 1
500 j
500 j
500 j
500 j
I
500 1
500 j
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 1
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500)
I
500 1
500 1
500 1
500 1
500 j
500 1
500 1
500 j
500 1
500 1
I
I
NI
# met a
12|
15|
14|
25 1
24 1
21 1
25 1
15|
16|
25 1
I
28|
24 1
25 1
26|
28 1
24 1
25 1
26|
32 1
19|
I
«|
32 1
15|
28 1
24 1
23 1
25 1
25 1
21
25 1
I
25 1
24 1
32 1
33 1
15|
15|
25 1
24 1
26 1
23|
I
25 1
14|
25 1
23 1
25 1
32 1
18|
17|
16|
«|
NI
percent
2.40|
3.00J
2.80J
5.00J
4.80|
4.20J
5.00J
3.00J
3. 20 j
s.ooj
1
5.60|
4.80J
5. 00 1
5. 20 j
5. 60 j
4. 80 j
s.ooj
5.20J
6. 40 j
3.80J
1
3.00|
6. 40 j
3.00J
5.60J
4. 80 j
4.60J
s.ooj
s.ooj
4.20J
s.ooj
1
5.00|
4.80J
6.40J
6.60J
3. 00 j
3.00J
S.OOj
4.80J
5.20J
4.60J
1
5.00|
2.80|
s.ooj
4.60|
s.ooj
6.40|
3.60|
3.40|
3.20|
3.00J
I
1
59
-------
Animal Slide
Scorer Treat.
Code
Sex
Dose
Sample Number chr-tid chr-some chr-tid
Time
Cells
break
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
:89
190
191
192
193
194
195
196
197
198
199
200
1074 1
1 1075|
1076|
1077)
1078J
1079 j
1080J
1081 1
1082J
1083 1
1
1084|
1085 j
1086J
1087J
1088 j
1089J
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2000 j
2000 1
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60
-------
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
'90
191
192
193
194
195
196
197
198
199
200
chr-some
break
o|
0|
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0|
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CA/cell
exc qao
0.080)
o|
0.040)
0.040 j
0.040 |
0.120 |
0.160)
0.240J
0|
0.080 |
I
0.160 |
0.240 j
0.400 j
0.120 j
0.120 |
0.480J
0.080J
0.320 j
0.240J
0.360)
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0.200]
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o.osoj
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3.040|
0.040|
0.040 |
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0.040)
0.160)
0.040)
1
1
cells w/
0 CA
23 1
25)
2*1
24)
2*1
2*1
2*1
23 1
25)
2*1
1
2*1
23)
23)
2*1
24 1
22)
2*1
23 1
23)
22)
1
2*1
23)
25 1
2*1
2*1
2*1
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2*1
25 1
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1
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2*1
22)
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21)
24 1
1
23)
25)
2*1
24 1
24 1
25 1
25)
24)
23|
2*1
1
1
cells w/
1 CA
2|
°l
1|
M
1|
0|
0|
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0|
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o|
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2|
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1|
0|
0|
1|
0|
1|
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!
cells w/
2 CA
0|
0|
°l
»l
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0|
o|
1|
o|
1|
1
°l
1|
0|
o|
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1|
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11
2)
o|
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0|
0|
0|
0)
0|
0|
0|
0|
2|
0|
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cells w/
3 CA
°l
0|
"I
0|
0|
1|
o|
0|
o|
0|
I
"I
0|
11
M
1|
1|
0|
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2|
3|
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0|
0|
0|
0|
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0|
0|
0|
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0|
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11
o|
o|
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2|
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0|
0|
1
°l
o|
0|
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0|
0|
o|
«l
o|
o|
1
1
cells w/
4 CA
o|
o|
0|
o|
0|
0|
11
11
»l
o|
I
11
11
o|
o|
0|
M
0|
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1
o|
0|
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0|
0|
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0|
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0|
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o|
o|
0|
0|
0|
o|
1
°l
o|
0|
o|
o|
0|
o|
0|
0|
0|
1
1
cells w/
5 CA
o|
°l
0|
0|
"1
°l
0|
"1
«l
«l
1
°l
o|
o|
0|
0|
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o|
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0|
o|
1
o|
o|
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°l
0|
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0|
0|
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0|
1
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11
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o|
0|
0|
0|
o|
11
1
0|
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0|
0|
0|
o|
o|
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o|
0|
1
!
61
-------
151
1S2
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
cells w/
6 CA
0|
o|
o|
0|
°l
0|
o|
0|
»l
0|
1
°l
"1
0|
o|
°l
°l
»l
1|
o|
0|
1
o|
°l
o|
°l
0|
0|
0|
o|
"1
0|
1
o|
o|
o|
01
«l
0|
°l
0|
o|
«l
1
0|
0|
0|
0|
0|
°l
0|
o|
0|
0|
1
!
cells u/
7 CA
o|
o|
o|
o|
0|
o|
o|
0|
o|
°l
1
"1
o|
11
o|
o|
"I
o|
0|
0|
°l
1
o|
°l
0|
°l
"I
0|
o|
0|
0|
0|
1
0|
of
0|
o|
°l
0|
0|
o|
0|
0|
1
0|
0|
0|
0|
o|
0|
o|
o|
°l
o|
1
1
cells u/
8 CA
°l
0|
»l
°l
0|
o|
o|
o|
o|
0|
1
0|
o|
o|
o|
"1
0|
0|
°l
o|
o|
1
o|
o|
0|
o|
0|
°l
0|
°l
0|
0|
1
0|
o|
0|
0|
°l
0|
o|
o|
of
°l
1
0|
0|
0|
°l
°l
0|
0|
o|
0|
0|
1
1
cells u/
9 CA
0|
°l
0|
o|
°l
"1
0|
o|
0|
0|
1
o|
o|
°l
o|
0|
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o|
o|
o|
o|
1
"1
0|
0|
0|
0|
o|
o|
o|
0|
0|
1
o|
0|
o|
o|
0|
0|
°l
°l
0|
°l
1
o|
0|
°l
°l
o|
0|
o|
°l
0|
0|
1
1
cells w/
10+ CA
°l
o|
o|
0|
o|
°l
°l
0|
°l
"1
1
o|
0|
0|
o|
0|
"1
0|
o|
0|
o|
1
°l
0|
0|
o|
°l
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0|
0|
"I
o|
1
0|
0|
0|
o|
0|
o|
0|
o|
"1
o|
1
0|
0|
0|
o|
0|
0|
0|
o|
0|
0|
1
I
pet. CA
positive
8.0|
o.oj
4.0|
4.0|
4.0|
4.0|
4.0|
8.0|
0.0|
4.0|
1
4.0|
a.o|
B.0|
4.0|
4.0|
12. 0|
4.0|
8.0|
8.0J
12. OJ
1
4.0|
8.0)
o.oj
4.0|
4.0|
4.0|
4.0|
4.0|
0.0|
4.0|
1
4.0|
4.0|
8.0|
o.oj
4.0|
4.0|
12.0|
4.0|
16. 0|
4.0|
I
8.0|
3.0|
4.0|
4.0|
4.0|
0.0|
o.oj
4.0|
8.0|
4.0|
I
I
MI
# cells
500 1
500 j
500 j
500 j
500 j
500 j
500 1
500 j
500 j
500 1
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 1
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 j
50CI
500 1
500 j
500 1
500 j
I
I
MI
» meta
25 1
35 1
32 1
24 1
26 1
«l
18|
17|
16|
14|
1
12|
10|
15|
18|
10|
9\
5|
21
1|
B|
I
25 1
26|
24 1
25 1
28|
32 1
15|
24 1
25 1
«|
I
25 1
15|
14|
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12)
"I
12|
13|
10|
2|
1
25 1
32 1
25 1
23 1
21 1
24 1
25 1
25 1
25 1
21 1
I
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MI
percent
5. 00 1
7. 00 1
6.40J
4.80J
5. 20 1
3. 00 1
3. 60 1
3.40J
3.20J
2.80J
I
2.40|
2.00J
3.00J
3.60J
2.00J
1.80 1
1.00J
0.40J
0.20 1
1.60 j
I
5.00|
5.20J
4.80J
s.ooj
5.60J
6.40J
3. 00 j
4.80J
s.ooj
3.00J
I
5.00|
3. 00 1
2.80J
3.00J
2.40J
2.20J
2.40J
2.60J
2.00J
0.40J
I
5.00J
6.40J
5. 00 1
4.60|
4.20J
4.80|
S.OOj
s.ooj
S.OOj
i.20|
I
I
62
-------
201
202
203
204
205
206
207
208
209
210
211
212
213
2H
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
:44
245
246
247
248
249
250
Animal Slide
1124 1
1125 j
1126)
1127J
1128 j
1129 j
1130 j
1131J
1132 j
1133J
I
1134 1
1135 j
1136 j
1137 1
1138 j
1139)
1140 j
1141J
1142 1
1143 1
I
1144)
1145|
1146|
1147|
1148|
1149|
1150|
1151J
1152|
1153|
1
1154|
1155|
1156|
1157|
1158 j
1159 1
1160 1
1161 j
1162 1
1163J
I
1164)
1165|
1166J
1167 1
1168J
1169|
1170 1
1171|
1172|
1173|
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BN|
BN|
BN|
I
8N|
BN|
BN|
8N|
BN|
BN|
BN|
BNJ
BN|
BN|
I
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
BNJ
BN|
I
I
Treat.
Code
t
t
t
t
t
t
t
t
t
t
c
c
c
c
c
t
t
t
t
t
t
t
t
t
t
t
t
t
t
t
c
c
c
c
c
t
t
t
t
t
t
t
t
t
t
t
t
t
t
t
Sex
I f|
I f|
I *\
I f|
I *l
I f|
I f|
I f|
I f|
I f|
I I
f|
-------
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
chr-some
break
o|
°l
°l
o|
°l
1|
0|
°l
o|
o|
I
°l
o|
o|
0|
o|
°l
°l
0|
o|
o|
I
11
o|
0|
o|
M
11
o|
0|
o|
11
I
°l
o|
°l
0|
°l
o|
o|
0|
0|
o|
I
11
o|
°l
o|
°l
11
°l
°l
o|
o|
I
I
ehr-tid
exchange
o|
°l
o|
11
°l
0|
o|
o|
0|
11
1
"1
°l
o|
o|
o|
°l
o|
°l
o|
0|
1
°l
o|
0|
o|
11
0|
o|
o|
o|
0|
I
°l
0|
°l
o|
°l
°l
°l
°l
o|
°l
I
°l
0|
°l
o|
°l
°l
°l
°l
0|
2|
I
I
chr-some
exchange
o|
o|
o|
°l
°l
o|
°l
"I
0|
0|
I
"I
°l
0|
o|
°l
"I
°l
"I
o|
o|
I
°l
o|
0|
o|
o|
0|
o|
°l
o|
0|
I
°l
°l
"I
o|
o|
°l
°l
0|
o|
o|
I
°l
°l
°l
°l
0|
°l
0|
°l
o|
o|
I
I
other
aberratn
°l
0|
°l
o|
0|
o|
°l
o|
0|
°l
I
°l
o|
0|
"I
°l
o|
°l
o|
"I
0|
I
0|
o|
o|
o|
o|
0|
0|
°l
o|
o|
I
0|
0|
0|
°l
o|
°l
°l
o|
o|
o|
I
°l
°l
o|
.'!
0|
°l
0|
0|
o|
°l
I
I
Ok/cell
exc gap
0.040]
0.040 j
0.080 |
0.360 j
0.040 j
0.080 |
0.120 |
0.160 |
0.120)
0.080 |
I
. 0.120)
0|
o|
0.040)
°l
0.080)
0.040 j
o|
0.080 |
0.040)
I
0.080)
0.040)
0.080)
0.120)
0.120)
0.240 |
0.160)
0.200 |
o|
0.240)
I
0)
0.080)
o|
0.080)
0.040 1
0.040)
0.040)
0.040)
0.080)
°l
I
0.120)
0.120)
0.120)
0.040)
01
0.240)
0.120)
0.040)
0.160)
0.iOOJ
I
I
cells w/
0 CA
24 1
24 1
24 1
22 1
24 1
24 1
24)
24)
24 1
24 1
I
22 1
25 1
25 1
24 1
25 1
23)
24 1
25 1
24 1
24 1
I
24)
24 1
24 1
24 1
24)
23)
24)
24 1
25 1
24)
I
25 1
23|
25 1
23|
24)
24)
24 1
24)
24)
25)
I
24)
24)
24)
24)
25 1
24)
24)
24)
23|
24 1
I
I
cells w/
1 CA
1|
1|
0)
°l
1|
°l
»l
o|
0|
o|
I
3|
o|
o|
1|
o|
2)
1|
o|
0|
1|
I
0)
II
0|
o|
o|
o|
"1
°l
o|
o|
1
o|
2|
o|
21
1)
1|
1|
11
0|
o|
I
o|
o|
0)
1|
o|
°l
o|
1|
1|
o|
I
I
cells u/
2 CA
°l
o|
M
°l
o|
M
o|
0|
o|
M
I
o|
o|
0|
o|
o|
o|
o|
o|
M
°l
I
1|
o|
11
o|
o|
°l
o|
o|
o|
o|
I
0)
0|
"I
°l
0|
o|
o|
0)
11
o|
o|
0)
°l
0|
0|
°l
0)
0|
o|
o|
cells w/
3 CA
0|
0|
°l
3|
0|
"I
11
o|
11
"I
I
o|
o|
o|
o|
0|
"I
o|
°l
o|
0|
I
o|
o|
0|
11
M
2|
o|
"I
o|
°l
1
°l
o|
o|
o|
o|
o|
o|
o|
0|
°l
1
11
1)
11
0|
°l
o|
11
0)
11
0|
I
I
cells w/
4 CA
0)
"I
°l
"I
0)
°l
o|
1|
0|
o|
I
"I
0|
o|
o|
o|
"I
0)
o|
o|
°l
I
°l
o|
0|
o|
o|
°l
11
0|
o|
o|
I
0|
o|
0|
0|
°l
o|
. o|
0|
°l
°l
I
o|
o|
0)
°l
o|
"I
o|
°l
°l
o|
I
I
cells y/
5 CA
o|
°l
o|
o|
o|
°l
o|
o|
o|
o|
I
°l
o|
o|
0|
o|
0|
o|
0|
ol
o|
I
o|
o|
0)
o|
o|
o|
o|
11
o|
o|
I
0|
0|
o|
o|
o|
o|
o|
o|
0|
o|
I
o|
o|
0)
°l
o|
o|
0|
0|
o|
M
I
I
64
-------
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
2J9
240
241
242
243
244
245
246
247
243
249
25 0
cells w/
6 CA
»l
0|
o|
°l
°!
o|
o|
o|
o|
"I
1
o|
o|
o|
0|
o|
0|
o|
0|
°l
o|
1
0|
°l
°l
"1
0|
"I
o|
"1
0|
11
1
0|
01
0|
0|
0|
0|
0|
0|
o|
Of
1
0|
01
°l
0|
•M
11
0|
0|
«l
0|
1
1
cells w/
7 CA
01
0|
0|
0|
o|
»l
°l
0|
o|
"1
1
o|
»l
0|
o|
0|
°l
0|
0|
0|
o|
1
0|
o|
°l
o|
°l
°l
°l
o|
o|
o|
1
o|
°l
°l
0|
0|
0|
°l
°l
0|
o|
1
3|
0|
°l
°!
31
1
°l
°l
°l
0|
°l
1
1
cells w/
8 CA
«l
0|
0|
°l
o|
0|
°l
°l
°l
°l
I
o|
o|
o|
o|
o|
0|
0|
0|
o|
°l
1
o|
o|
0|
0|
0|
o|
o|
o|
»l
o|
1
o|
°l
o|
o|
0|
°l
0|
0|
o|
°l
1
o|
o|
0|
01
0|
°l
0|
o|
0|
o|
1
1
cells u/
9 CA
o|
o|
01
"1
0|
0|
0|
»l
«l
0|
1
0|
"I
o|
°l
»l
0|
«l
0|
0|
°l
1
0|
o|
o|
o|
o|
o|
o|
»l
0|
o|
1
o|
o|
0|
0|
o|
0|
0|
0|
0|
»l
1
0|
o|
0|
0|
o|
"I
o|
"1
0|
o|
1
1
cells w/
10+ CA
o|
°l
0|
0|
0|
o|
o|
0|
0|
0|
I
0|
o|
o|
"I
0|
0|
o|
0|
o|
0|
I
0|
o|
o|
0|
»l
"I
o|
°l
0|
o|
I
0|
°l
o|
0|
0|
0|
°l
0|
o|
°l
I
0|
»l
0|
0|
0|
o|
0|
0|
o|
0|
I
I
pet. CA
positive
4.0|
4.0|
4.0|
12. 0|
4.0J
4.0|
4.0J
4.0|
4.0|
4.0|
I
12. 0|
o.oj
o.oj
4.0|
0.0|
8.0J
4.0|
0.0|
4.0|
4.0|
I
4.0|
4.0|
4.0|
4.0|
4.0|
a.o|
4.0|
4.0|
0.0|
4.0|
I
0.0|
s.oj
o.oj
8.0J
4.0|
4.0|
4.0|
4.0|
4.0|
o.oj
I
4.0|
4.0|
4.0|
4.0|
0.0|
4.0|
4.0|
4.0|
8.0|
4.0|
I
I
MI
# cells
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 1
500 j
500 j
500 1
500 j
500 j
I
I
MI
# meta
20 1
30 1
21 1
25 1
21 1
25 1
32 1
14|
18|
19|
I
15|
19|
32|
35 1
35 1
«|
25 1
24 1
28|
24 1
1
26 1
27|
42|
15|
25 1
32|
25 1
32 1
15|
24 1
I
25 1
26 1
24 1
25 1
32 1
32 1
21 1
20 1
19|
18|
1
20 1
25 1
21 1
24 1
19|
18|
25 1
26 1
15|
14|
1
1
MI
percent
4.00J
6.00J
4.20J
5.00J
4. 20 j
5.00J
6.40J
2.80J
3.60J
3.80J
1
3.00|
3.80J
6.40J
7.00J
7.00J
3.00J
5. 00 j
4. 80 j
5. 60 j
4.80|
1
5.20|
5. 40 j
8.40J
3.00J
s.ooj
6. 40 j
s.ooj
6. 40 j
3.00J
4.80J
1
s.ooj
5.20J
4.80J
5. 00 1
6.40J
6.40J
4.20|
4.00|
3.80J
3.60J
1
4.00|
5.00|
4.20|
4.80|
3.80|
3.60|
5.00|
5.20|
3.00|
2.80|
1
1
65
-------
251
252
253
254
255
256
257
258
259
260
Animal Slide Scorer Treat. Sex Dose Sample Number chr-tid chr-some chr-tid
Code Time Cells qao aao break
1174J
1175J
1176J
1177)
1178J
1179J
1180 1
1181 1
1182 1
1183 j
I
I
B| BN|
B| BN|
B| BN|
B| BN|
B| BN|
B| BH|
B
B
B
B
BN|
BN|
BN|
BN|
I
1
Pi
Pi
Pi
Pi
Pi
Pi
Pi
Pi
Pi
Pi
1
1
m| 12. S|
m| 12. 5|
mj 12. 5|
m| 12.5|
m| 12.5|
f| 12.5|
fj 12. 5|
fj 12.5J
fj 12. 5|
fj 12. 5J
I I
I I
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48|
48 1
I
t
25 1
25 1
25 1
25)
25 1
25 1
25 1
25 1
25 1
25 1
I
I
0|
3|
°l
1|
0|
0|
5|
0|
0|
4I
1
1
o|
0|
o|
0|
0|
°l
Q I
Q 1
0|
0|
1
1
'1
n
5|
0|
M
4|
3|
7|
5|
5|
1
1
66
-------
251
252
253
254
255
256
257
258
259
260
chr-some chr-tid chr-some
break exchange exchange
2| 3| 0|
3| 1| 0|
1| 0| 0|
0| 0| 0|
0| 0| 0|
1| 0| OJ
0[ 0| 0|
0| 0| 0|
o| oj o|
0| 0| 0|
I I I
I I '
other CA/cell
aberratn exc gap
0| 0.560]
OJ 0.440]
OJ 0.240J
0| OJ
OJ 0.160J
OJ 0.200]
0| 0.120]
OJ 0.280]
OJ 0.200J
OJ 0.200J
I I
I I
cells w/ cells w/
0 CA 1 CA
20] 0
22] 0
24] 0
25 j 0
24] 0
24] 0
24 j 0
23 j 0
24 j 0
24] 0
I
I
cells w/ cells w/ cells
2 CA 3 CA 4 CA
3| 0|
1| 0|
0| 0|
0| 0|
0| 0|
0| 0|
0| 1|
0| 1|
0| 0|
0| 0|
I
I
w/ cells w/
5 CA
2| 0|
1| 1]
0| 0|
0| 0|
1| 0|
0] 1]
0| 0|
1 0|
1| 0|
0| 1|
I
I
67
-------
cells u/ cells u/ cells w/ cells w/ cells w/ pet. CA
6 CA 7 CA 8 CA 9 CA 10* CA positive *
251
252
253
254
2SS
256
257
258
259
260
°l
0|
1|
o|
°l
o|
o|
o|
o|
0|
I
I
o|
0|
0|
o|
o|
o|
"I
o|
°l
0|
I
I
°l
0|
o|
o|
o|
"I
"I
°l
o|
o|
I
I
0|
0|
°l
0|
o|
0|
0|
o|
o|
o|
I
I
°l
o|
o|
o|
o|
0|
°l
0|
o|
0|
I
I
20.0 1
12. 0|
«.0|
0.0|
4.0|
4.0|
4.0|
8.0|
4.0|
4.0|
I
I
MI HI HI
cells # met a percent
500 1
500 1
500)
500 1
500 1
500 j
500 j
500 1
500 1
500 1
I
I
15|
25 1
32 1
32 1
25 1
24 1
26 1
28|
29 1
27|
1
1
3.00|
5. 00 j
6.40|
6.40J
5. 00 1
4.80J
S.20|
5.60J
5.80J
5. 40 j
1
1
68
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Pet. Mean Pet.
1 3 0.0 24.0 m 60.000 18.400
2 133 0.0 24.0 m 80.000 20.000
69
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 7.945 24 0.9991
Sex 7.641 12 0.8125
Time 3.116 8 0.9269
70
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
24.00 (Males) for 067889
0.486
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
13
6
8
13
Cells
Scored
200
250
250
250
Percent
Aberrant
6.5000
2.4000
3.2000
5.2000
SEM
(for Obs)
1.0522
0.6532
0.5333
0.8537
Pairwise
Significance
0.9842
0.9504
0.7215
Time = 48.00 (Males) for 067889
P
alpha
0.095
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
12
9
11
17
Cells
Scored
250
250
250
250
Percent
SEM
Pairwise
Aberrant (for Obs) Significance
4.8000
3.6000
4.4000
6.8000
0.8000
0.7180
0.7180
1.0414
0.7482
0.5845
0.1694
71
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
72.00 (Males) for 067889
0.110
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
11
7
9
15
Cells
Scored
250
250
250
250
Percent
Aberrant
4.4000
2.8000
3.6000
6.0000
SEM
(for Obs)
0.7180
0.6110
0.7180
1.3663
Pairwise
Significance
0.8315
0.6760
0.2102
Time
P
alpha
24.00 (Females) for 067889
0.381
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
fJOO.OO
1000.00
2000.00
Cells
10
7
12
10
Cells
Scored
250
250
250
250
Percent
SEM
Aberrant (for Obs)
4.0000
2.8000
4.8000
4.0000
0.5963
0.8537
0.9978
0.0000
Pairwise
Significance
0.7704
0.3314
0.5000
72
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
48.00 (Females) for 067889
P
alpha
0.348
w • ^ ~m ^
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
7
10
12
9
Cells
Scored
250
250
250
250
Percent
Aberrant
2.8000
4.0000
4.8000
3.6000
SEM
(for Obs)
1.3400
0.5963
0.8000
0.7180
Pairwise
Significance
0.2296
0.1211
0.3057
Time
P
alpha
72.00 (Females) for 067889
0.348
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
10
8
9
11
Cells
Scored
250
250
250
250
Percent
Aberrant
4.0000
3.2000
3.6000
4.4000
SEM
(for Obs)
1.3333
0.5333
0.4000
0.4000
Pairwise
Significance
0.6844
0.5925
0.4118
73
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Pet. Mean Pet.
1 3 0.0 24.0 m 60.000 18.400
2 133 0.0 24.0 m 80.000 20.000
74
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 7.945 24 0.9991
Sex 7.641 12 0.8125
Time 3.116 8 0.9269
75
-------
filename: C:\CA\CATEST02.ILS date: time:
Both sexes (All times) for 067889
p = 0.062
alpha = 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant Cells Percent SEM Pairwise
MG/KG Cells Scored Aberrant (for Obs) Significance
0.00 63 1450 4.3448 0.4207
500.00 47 1500 3.1333 0.2705 0.9587
1000.00 61 1500 4.0667 0.2929 0.6467
2000.00 75 1500 5.0000 0.3636 0.1998
76
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (pet. damaged cells)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Pet. Mean Pet.
1 3 0.0 24.0 m 60.000 18.400
2 133 0.0 24.0 m 80.000 20.000
77
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time = 48.00 (Males) for 067889
alpha = 0.050
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
(with positive controls)
Aberrant Cells Percent SEM
MG/KG Cells Scored Aberrant (for Obs)
0.00 12 250 4.8000 0.8000
12.50 18 250 7.2000 1.8667
Pairwise
Significance
0.1293
Time = 48.00 (Females) for 067889
alpha = 0.050
One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
(with positive controls)
Aberrant Cells Percent SEM
MG/KG Cells Scored Aberrant (for Obs)
0.00 7 250 2.8000 1.3400
12.50 13 250 5.2000 0.6110
Pairwise
Significance
0.0855
78
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Abs/Cell Av Abs/Cell
1 3 0.0 24.0 m 0.600 0.184
2 133 0.0 24.0 m 0.800 0.200
79
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 26.414 24 0.3325
Sex 7.181 12 0.8454
Time 17.934 8 0.0217
80
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
24.00 (Males) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 13
500.00 8
1000.00 15
2000.00 37
Cells Aberrat'ns
SEM
Pairwise
Scored
200
250
250
250
/ Cell (for Obs) Significance
0.0650
0.0320
0.0600
0.1480
0.0105
0.010
0.0149
0.0338
0.9463
0.5837
0.0043
Time = 48.00 (Males) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 14
500.00 20
1000.00 37
2000.00 60
Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell (for Obs) Significance
0.0560
0.0800
0.1480
0.2400
0.0107
0.0239
0.0358
0.0404
0.1517
0.0006
0.0000
81
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
72.00 (Males) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 13
500.00 9
1000.00 22
2000.00 35
Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell
0.0520
0.0360
0.0880
0.1400
(for Obs)
0.0104
0.0093
0.0259
0.0322
Significance
0.8031
0.0641
0.0007
Time = 24.00 (Females) for 067889
P
alpha
0.001
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 10
500.00 9
1000.00 21
2000.00 25
Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell (for Obs) Significance
0.0400
0.0360
0.0840
0.1000
0.0060
0.0151
0.0334
0.0123
0.5907
0.0241
0.0056
82
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
48.00 (Females) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 7
500.00 17
1000.00 27
2000.00 34
Cells Aberrat'ns
Scored
250
250
250
250
/ Cell
0.0280
0.0680
0.1080
0.1360
SEM
(for Obs)
0.0134
0.0120
0.0179
0.0305
Pairwise
Significance
0.0206
0.0003
0.0000
*
*
*
Time
P
alpha
72.00 (Females) for 067889
0.000
- 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 10
500.00 11
1000.00 19
2000.00 35
Cells Aberrat'ns
SEM
Scored
250
250
250
250
/ Cell
0.0400
0.0440
0.0760
0.1400
(for Obs)
0.0133
0.0093
0.0139
0.0225
Pairwise
Significance
0.4136
0.0473
0.0001
83
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Abs/Cell Av Abs/Cell
1 3 0.0 24.0 m 0.600 0.184
2 133 0.0 24.0 m 0.800 0.200
84
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
Variance inflation factor: 1.000
Alpha: 0.050
Factor Deviance df p
Scorer 26.414 24 0.3325
Sex 7.181 12 0.8454
Time 17.934 8 0.0217
85
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time = 24.00 (Both sexes) for 067889
P
alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 23
500.00 17
1000.00 36
2000.00 62
Cells Aberrat'ns
SEM
Pairwise
Scored
450
500
500
500
/ Cell
0.0511
0.0340
0.0720
0.1240
(for Obs)
0.0063
0.0088
0.0180
0.0183
Significance
0.9003
0.0985
0.0001
Time
P
alpha
48.00 (Both sexes) for 067889
0.000
= 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total
MG/KG Aberrat'ns
0.00 21
500.00 37
1000.00 64
2000.00 94
Cells Aberrat'ns
SEM
Pairwise
Scored
500
500
500
500
/ Cell
0.0420
0.0740
0.1280
0.1880
(for Obs)
0.0089
0.0131
0.0200
0.0274
Significance
0.0178
0.0000
0.0000
86
-------
filename: C:\CA\CATEST02.ILS date: time:
Time = 72.00 (Both sexes) for 067889
p = 0.000
alpha = 0.040 (scaled by .8, assuming next run to exclude high dose)
One tailed test (Poisson) for chromosomal aberration (total aberrations)
Total Cells Aberrat'ns SEM Pairwise
MG/KG Aberrat'ns Scored / Cell (for Obs) Significance
0.00 23 500 0.0460 0.0083
500.00 20 500 0.0400 0.0065 0.6763
1000.00 41 500 0.0820 0.0144 0.0122 *
2000.00 70 500 0.1400 0.0191 0.0000 *
87
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Chromosomal aberration (total aberrations)
There are 2 outliers at the .05 significance level.
Line Dose Time Sex Abs/Cell Av Abs/Cell
1 3 0.0 24.0 m 0.600 0.184
2 133 0.0 24.0 m 0.800 0.200
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time =
alpha
48.00 (Males)
0.050
for 067889
One tailed test (Poisson) for chromosomal aberration (total aberrations)
(with positive controls)
Total
MG/KG Aberrat'ns
0.00 14
12.50 76
Cells Aberrat'ns
Scored / Cell
250 0.0560
250 0.3040
SEM
(for Obs)
0.0107
0.0560
Pairwise
Significance
0.0000
Time =
alpha
48.00 (Females)
= 0.050
for 067889
One tailed test (Poisson) for chromosomal aberration (total aberrations)
(with positive controls)
Total
MG/KG Aberrat'ns
0.00 7
12.50 58
Cells Aberrat'ns
Scored / Cell
250 0.0280
250 0.2320
SEM
(for Obs)
0.0134
0.0259
Pairwise
Significance
0.0000
89
-------
filename: C:\CA\CATEST02.ILS date: time:
Endpoint: Mitotic Index
Variance inflation factor: 1.341
Alpha: 0.050
Factor Deviance df p
Scorer 52.058 24 0.0008
Sex 206.145 24 0.0000
Time 136.872 32 0.0000
90
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
24.00 (Males) for 067889
0.768
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
196
218
213
202
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
3.9200
4.3600
4.2600
4.0400
SEM
(for Obs)
0.5531
0.3874
0.2876
0.3745
Pairwise
Significance
0.8298
0.7707
0.6045
Time
P
alpha
48.00 (Males) for 067889
0.000
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
264
179
140
64
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.2800
3.5800
2.8000
1.2800
SEM
(for Obs)
0.3492
0.1849
0.1789
0.1937
Pairwise
Significance
0.0002
0.0000
0.0000
91
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
72.00 (Males) for 067889
= 0.000
= 0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
230
206
152
76
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.6000
4.1200
3.0400
1.5200
SEM
(for Obs)
0.3876
0.3441
0.2596
0.2585
Pairwise
Significance
0.1551
0.0002
0.0000
Time = 24.00 (Females) for 067889
P
alpha
0.697
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
256
241
236
227
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.1200
4.8200
4.7200
4.5400
SEM
(for Obs)
0.2313
0.1093
0.1794
0.3724
Pairwise
Significance
0.2756
0.2123
0.1214
92
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time
P
alpha
48.00 (Females) for 067889
0.572
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
253
239
232
219
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.0600
4.7800
4.6400
4.3800
SEM
(for Obs)
0.4927
0.3299
0.5504
0.4263
Pairwise
Significance
0.2881
0.1993
0.0831
Time
P
alpha
72.00 (Females) for 067889
0.707
0.050
Likelihood ratio for mitotic index
MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
222
212
240
224
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.4400
4.2400
4.8000
4.4800
SEM
(for Obs)
0.4020
0.3180
0.1978
0.3593
Pairwise
Significance
0.3359
0.7705
0.5333
93
-------
filename: C:\CA\CATEST02.ILS date:
time:
Time = 48.00 (Males) for 067889
alpha = 0.050
Likelihood ratio for mitotic index (with positive controls)
Cells in Cells Mitotic SEM
MG/KG Mitosis Scored Index (%) (for Obs)
0.00 264 5000 5.2800 0.3492
12.50 243 5000 4.8600 0.4483
Pairwise
Significance
0.1692
Time = 48.00 (Females) for 067889
alpha = 0.050
Likelihood ratio for mitotic index
(with positive controls)
MG/KG
0.00
12.50
Cells in
Mitosis
253
253
Cells
Scored
5000
5000
Mitotic
Index (%)
5.0600
5.0600
SEM
(for Obs)
0.4927
0.1492
Pairwise
Significance
0.5000
94
------- |