United States
             Environmental Protection
             Agency
             Environmental Monitoring
             Systems Laboratory
             P.O. Box 93478
             Las Vegas NV 89193-3478
EPA/600/8-90/086
December 1990
             Research and Development
&ER&
Chromosomal Aberration
Data Analysis and
Interpretation System
             User's Guide

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                         USER'S GUIDE


CHROMOSOMAL ABERRATION DATA ANALYSIS AND INTERPRETATION SYSTEM


                         Version 1.0

                        September 1990
                          Written by

                 Integrated Laboratory Systems
                        P.O. Box 13501
               Research Triangle Park, NC 27709
                    Contract No.  68-C8-0068
                        Project Officer

                       Charles H.  Nauman
             Exposure Assessment Research Division
          Environmental Monitoring Systems Laboratory
                 Las Vegas, Nevada 89193-3478
         ENVIRONMENTAL MONITORING SYSTEMS LABORATORY
              OFFICE OF RESEARCH AND DEVELOPMENT
             U.  S.  ENVIRONMENTAL PROTECTION AGENCY
                 LAS VEGAS, NEVADA 89193-3478

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                            NOTICE

     The information in this document has been funded wholly
or in part by the United States Environmental Protection
Agency under Contract 68-C8-0068 to Integrated Laboratory
Systems.  It has been subjected to the Agency's peer and
administrative review, and it has been approved for
publication as an EPA document.  Mention of trade names or
commercial products does not constitute endorsement or
recommendation for use.
                          DISCLAIMER

     The Chromosomal Aberration Data Analysis and
Interpretation System software and documentation are provided
"as is" without guarantee or warranty of any kind, expressed
or implied.  The Environmental Monitoring Systems Laboratory,
U.S. Environmental Protection Agency, Las Vegas, NV, and
Integrated Laboratory Systems will not be liable for any
damages, losses, or claims consequent to the use of this
software or documentation.
                               ii

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                           ABSTRACT
     This user's manual provides guidance to researchers and
the regulatory community for interacting with a data analysis
and interpretation system, designated CA.  CA is a data
management and analysis system designed for chromosomal
aberration and mitotic index data collected using in vivo test
systems.  The objective in developing this system has been to
promote consistency and intercomparability of assay test
results across laboratories, thus providing researchers and
government decision makers with a means to assure comparable
analyses of test data.  The CA data analysis and
interpretation system has been developed in consultation with
a panel of biostatisticians and experts in the field of
cytogenetics.
                              111

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                           CONTENTS


Notice 	 ii
Abstract 	 iii
Contents 	 iv

Introduction 	 1

Part 1.  Installation 	 3

Part 2.  Operation 	 5

     About Menus and Forms 	 5
     CA' s Data Model 	 5
     Entering Data and Other Information 	 5
     The Main Menu 	 7

          0.  Leave CA	 7
          1.  Set Up 	 7
               1.1  Select Endpoints 	 7
               1.2  Optional Fields 	 8
          2.  Data Entry 	 8
               2.1  Experiment Description 	 8
               2.2  Spreadsheet 	 9
          3.  Disk I/O 	 13
               3.1  Recall 	 13
               3.2  Save 	 15
               3.3  Import and 3.4 Export 	 16
          4.  Analysis 	 16
               4.1  Statistics 	 16
               4.2  Graph 	 20
          5.  Utility 	 21
               5.1  List 	 21
               5.2  Clear Session 	 22
               5.3  Sort 	 22
          6.  Miscellaneous  (Misc.) 	 23
               6.1  Key Field Search 	 23
               6.2  GLP Log	 24

     Sample Data Sets 	 24

Figures 	  26-49

Appendix 1.  Chromosomal Aberration Assay Software Development
               Panel Members.
Appendix 2.  Sample Test Data.
                               iv

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                           Introduction

     Structural chromosomal aberrations are broadly defined as
alterations in chromosome morphology.  These alterations are
assessed usually in cells at metaphase,  but certain kinds of
chromosomal damage can be detected during anaphase (e.g.,
anaphase bridges) or at interphase (micronuclei).   Most
chromosomal aberrations are deleterious and result in cell death.
However, some types (e.g., reciprocal translocations, small
deletions, inversions) can lead to altered gene function(s)
without an accompanying loss in cell viability.  Alterations in
gene function occurring as a result of interchanges between
specific chromosome regions are correlated with the appearance of
several different kinds of cancers ,  indicating the probable
involvement of these events in carcinogenesis.  Consistent with
this relationship, chromosomal aberrations are induced by many
known mutagens and carcinogens.   These  findings make the
analysis of chromosomal aberrations a useful indicator of
genotoxic damage in proliferating cell systems.

     CA is a data management and analysis system designed for
chromosomal aberration and mitotic index data collected using in
vivo test systems.  The software consists of a set of routines
for 1) entering, editing and storing experimental data and
descriptive information; 2) generating statistics appropriate to
the analysis of chromosomal aberrations and micronucleus index
data; and 3) presenting the results of these statistics through
graphs and tables.  CA was developed in consultation with a panel
of biostatisticians and experts in the field of cytogenetics (see
Appendix 1).

     This user's manual consists of two parts.  The first
describes the CA Installation program provided on the program
disk.  This description should be read carefully to ensure that
installation is performed correctly.  The second part describes
      Schwab and Amler  (1990)  Genes, Chromosomes  and Cancer, 1,
181.

     2Preston,  R.J.,  Au, W.,  Bender, M.A., Brewen, J.G., Carrano,
A.V., Heddle, J.A., McFee,  A.F., Wolff, S., and Wassom, J.S.  (1983)
Mutat. Res.  87:143-188.

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how to use CA.  The
description follows the organization of the program, describing
the main menu first, followed by the individual routines
corresponding to each of the menu entries.

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                      Part  l:  Installation

     CA is intended to run on an IBM PC compatible with a hard
disk under DOS.  The hard disk is assumed to be the C drive.
Throughout this manual,  words bracketed by < and > signify single
keystrokes of the named key.

     CA is distributed on two disks.  Disk 1 contains the program
itself.  Disk 2 contains the help files and sample data.  Each
disk contains an installation batch file as well.  Installation
is simple:

     At the C:\ prompt,  insert the distribution disk 1 in drive A
and type

                         A:INSTALL1 

     INSTALLl will first create the directory C:\CA and will then
copy the executable file CA.EXE into it.  When this is done, you
will be prompted to insert distribution disk 2 and type

                         A:INSTALL2 

     INSTALL2 will create the C:\CA\HELP subdirectory and will
then copy the help files into it.   The sample data can be
copied to the CA directory by typing at the C:\ prompt

                         copy A:\*.ILS C:\CA 

     To start CA, go to the CA directory by typing

                         CD\CA 
                         CA 
      CA may be run from any directory or subdirectory.  To install
CA in  another directory,  1) copy CA.EXE  into the directory from
which  it is  to be run,  2)  make a subdirectory named HELP, and 3)
copy the help files from the  distribution  diskette into the new
HELP subdirectory.

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     The first command changes the directory,  the second begins
the program.

     Initially, an introductory screen will appear noting the
program's sponsor, authors and version number, and prompting for
a user ID.  If nothing appears on the screen,  confirm 1) that the
CA directory exists, and 2) that CA.EXE and the HELP subdirectory
reside within it.  If not, repeat the installation procedure.  If
the file structure appears correct but the program will not run,
contact the Data Management Section at Integrated Laboratory
Systems (919 - 544-4589) for assistance.

     After the introductory screen appears and the user's ID has
been entered, press any key to bring up the main menu (Fig. 1).

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                        Part 2: Operation

About Menus and Forms

     The main menu is a single selection menu.  Select an item by
using the arrow keys to move the highlight bar and then press
.  When a selection is made, the menu closes and the
selected operation begins.

     Some menus permit multiple selections.  If multiple
selections are permitted,  will flag or unflag the
highlighted item but will not close the menu.  Multiple-selection
menus are closed with a special menu item or the ESCAPE 
key.

     The form screens are data entry devices.  They consist of a
set of  (usually labelled) fields into which text may be entered.
Selecting a field is much like selecting a menu item.  To enter
text into a field, move the cursor to the field using the arrow
keys and type in the appropriate text.

     Some screens are part menu and part form.  Treat the menu
portion as a multiple entry menu and the form portion as a text
entry screen.

CA's Data Model

     Each CA session maintains two distinct datasets.  The first
is a multi-page fixed form containing descriptive information
relating to several aspects of the experiment.  The second is a
spreadsheet for the experimental data, where the rows represent
observations and the columns represent fields.  This spreadsheet
has a number of fixed fields and a number of optional fields that
may be selected to tailor a session to the nature of the
experiment.  Help screens are available within each menu
selection through the Fl key.

Entering Data and Other Information

     CA employs two line editors.  The first is a very simple
editor used for the spreadsheet  (except for the remarks field)

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and various parameter entry fields on some control screens.
These are short fields and their values are simply replaced.
Entering new text into these fields clears the field and the  new
text appears as entered.   removes characters in the
order they were entered.

     Longer fields like DOS path prompts,  the fields comprising
the experiment description section, and the remarks fields of the
spreadsheet and the experiment description section employ a more
complete line editor. The control keys supported are ,
, , , , and the left and right
arrow keys.

      toggles between insert and overtype modes.  In
     insert mode, new text shifts existing text to the
     right.  In overtype mode, new text simply replaces the
     old.

      removes the character under the cursor and
     shifts the remaining text to the left.

      is similar to DELETE except that it removes
     the character to the left of the cursor.

      moves the cursor to the beginning of the field.

      moves the cursor to the end of the field.

     The left and right arrow keys move the cursor one
     character.  If the cursor is positioned under the first
     character, the left arrow key will close the window and
     move to the field to the left.  Similarly, if the
     cursor is positioned at the end of the text, the right
     arrow key will close the window and move to the field
     on the right.
     Remarks fields are found at the bottom of each page of the
experiment description forms and on the end of each row of the
data entry spreadsheet.  Moving the cursor into a remarks fields
opens a broad single line window.  If the field is not empty, the
cursor moves to the end of the existing text.  Any new text will

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be appended.  Moving out of the window  closes  the window,
displaying as much of the text as the standard form field width
will allow.  The hidden text is not lost and will become visible
again when the window is reopened.

The Main Menu

     All but one ('Leave CA1) of the sixteen items of the main
menu (Fig. 1) are grouped into six classes: Set Up,  Data Entry,
Disk I/O, Analysis, Utility, and Miscellaneous (Misc.)

0. Leave CA

     This is a special item outside the six functional categories
to be described.  Selecting this item will first prompt for
disposition of any unsaved changes and then return to DOS.

1. Set Up

     The setup routines (Endpoints and Optional Fields) determine
which of the non-default fields will appear on the spreadsheet.
•Endpoint' allows the selection of either or both the chromosomal
aberration and the mitotic index endpoints.  'Optional Fields'
provides a way to define up to fifteen additional data fields.

1.1 Select Endpoints

     This routine consists of a single screen  (Fig.  2A)
presenting the two types of endpoints CA supports: Chromosomal
Aberrations and Mitotic Index.  You may select either or both.
Endpoints are selected and deselected by moving the cursor
between  'Yes' and  'No1.  It is not necessary to press 
here.  To lock in the selection and return to the main menu, move
the cursor to the  'Go' button and press .  To ignore any
changes and return to the main menu, move to the 'Cancel1 button
      The left and right arrow keys usually move the cursor within
the text field of the remark, but if the cursor is at either edge
of the text, the corresponding arrow key can be used to leave the
field.  Note that in all cases, using an arrow key to  leave a field
assumes that there  is another field in the indicated direction to
move to.

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and press .

     Selecting 'Chromosomal Aberrations'  will activate twenty-one
columns.  These include nine columns for general categories of
various types of chromosomal aberrations and twelve columns for
the distribution of aberrations among cells.  Selecting 'Mitotic
Index1 will activate three columns.

1.2 optional Fields

     This selection presents a subscreen consisting of four
columns and fifteen lines (Fig. 3a) with which the user may name,
describe, and type up to fifteen additional fields.  A new field
is created (and subsequently appears in the spreadsheet) simply
by naming it.  The only restriction on the field names is that
they may not be duplicated.  'Name 1' will appear on the first
title line of the spreadsheet,  'Name 2' will appear on the
second.  There is no restriction on the description field; this
is for the user's convenience and may be omitted.  The fields are
assumed to contain integers unless otherwise defined.  To change
the default field types, go to the Type Field and make a
selection from the menu that appears when the field is entered
(Fig. 3b).  Currently, optional fields are not used in the
statistical analysis or in the graphs.  However, one category of
optional fields 'aberration1 can be used to insert specific
categories of chromosomal aberrations, not previously defined,
into that portion of the spreadsheet where such data are entered.
Data in these columns are used to calculate the locked field
•CA/cell1.  Note that the 'aberration1 field type will not appear
in the field type menu unless the  'chromosomal aberration' end
point has been previously selected.   returns to the main
menu.

2. Data Entry

     The data entry selections (Experiment Description and
Spreadsheet) are the means by which data are entered into CA
sessions by keyboard.   'Experiment Description1 consists of a six
page form with which to enter descriptive information pertaining
to the experiment, test article, solvent, positive control, test
system, and treatment.  The 'Spreadsheet1 allows access to the

                                8

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actual data.
2.1 Experiment Description

     This routine consists of a six page form presented in a
double window format (Figs. 4a-4f).  The left window contains the
current page of the form.  To enter the descriptions, use the
arrow keys to move between fields.   The right window contains an
index listing the six pages, highlighting the current page.  The
form opens displaying the experiment page.  Pages are changed
using the
PAGE-UP and PAGE-DOWN keys.  There are no entries among these
forms that are explicitly required before statistical analysis
can be conducted, but the contents of the test agent and dose
fields are used in labelling the statistics report.  If these
fields are blank, "?" will appear in their place.

2.2 Spreadsheet

     The spreadsheet consists of one thousand rows, eight
permanent columns (including a remarks field), and whatever
fields were defined by the setup routines.  Fifteen rows and
seven columns are visible onscreen at any one time.  The arrow
keys move the cursor a single row or column at a time, adjusting
the display when an attempt is made to move outside the current
7x15 window.   and  shift the display up and
down a page (fifteen lines).   and  shift the display left and right a page (seven columns).
 and  move to the first and last
pages of the spreadsheet.   and  move to the first and
last columns of the current row.   moves the cursor to the
first column of the next line.

     CA assumes that the three fields ANIMAL, SLIDE, and SCORER
will be the basic key fields and provides a small window in the
lower left corner of the spreadsheet where the values of these
fields for the current line are displayed.  This is for the
convenience of the user since whenever the window is shifted to
the right, at least one of the key fields is lost from view.

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     Fields are limited to nine characters except for the remarks
field which will accept eighty.  Entering text into the remarks
field opens a subwindow and activates the text editor.  On
closing the subwindow, only the first nine characters of the
remarks text will be displayed in the spreadsheet.  Each field
also has a fixed data type that is reflected in a small tag in
the lower right corner of the screen.  Edits to check for illegal
values are currently installed for the integer (whole number),
real (decimal), flag (yes/no),  sex (male/female), and treatment
type (treatment, control, positive-control) so attempts to enter
illegal values in these fields will be rejected.   The one
exception to this is a single period, which may be entered into
any numeric field to denote a missing value.

     The Sample Time field may be left blank but, if so, it must
be blank for every observation.  To CA, blank sample times imply
that time is not a factor and the analyses will be conducted
accordingly.  If only some Sample Time fields are blank, CA will
not know how to interpret them.

     Treatment Code may be left blank.  The accepted treatment
codes are 't1 for treatment group, 'p1 for positive control and
'c1 for solvent or negative control.   Records with blank
treatment codes are assumed to belong to the treatment group.

     Sex initially defaults to 'm1 (male), but this may be
changed with the F2 setup option described below.

     As stated in section 1.1,  selecting 'Chromosomal
Aberrations' while in 'Select Endpoints1 will activate twenty-one
columns in the spreadsheet.  The first nine columns  (plus any
additional optional columns specified for chromosomal
aberrations) are provided for entries on specific types of
chromosomal aberrations.  The ninth column of this set contains
the number of aberrations per cell (excluding gaps), including
any user defined aberration column(s).  Data in these columns are
not used in any of the subsequent statistical analyses conducted
on chromosomal aberration data, but are provided for the
convenience of the user.  The next eleven columns of this set are
used to indicate the number of cells with 0,1,2,3...9,10 or more
aberrations.  The last column contains the percentage of cells
                                10

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with at least one aberration.  The user has the option of
entering data which excludes or includes information on "gaps".
Data in these columns are used in the statistical analysis to
determine if the treatment increased significantly either the
frequency of damaged cells (i.e., the number of cells with at
least one chromosomal aberration, excluding or including gaps) or
increased significantly the number of aberrations among cells
(excluding or including gaps).

     When 'Mitotic Index1 has been selected, three columns are
activated in the spreadsheet.  These include two columns for data
entry, one for the total number of cells scored and one for the
number of cells at metaphase.  The third column contains the
mitotic index (cells at metaphase/total cells), presented as a
percentage.

     The ditto character (initially the double quote "), if
entered as the first character of a field, copies into the
current field the contents of the field immediately above it, and
then moves one space down.  The designated ditto character may be
toggled between the double quote and the single quote with the F2
setup option.  This option can also be used to toggle the cursor
movement between down and right.

      returns to the main menu.

     Fl accesses the Help screens for data entry.

     F2 opens a spreadsheet setup menu with nine selections
      (Fig. 5a).

     ASSIGN CURRENT COLUMN [fieldname]  (Fig. 5b) allows you
     to assign a constant value to all or part of the
     current column (Fig. 5c, note the default value of
     SEX).  The screen consists of three lines.  The first
     line accepts the value to be assigned.  The second
     specifies the range through which the value is to be
     set.  The default range is from the current row to the
     last non-empty row.  The third line allows you to
     either GO or CANCEL.
                                11

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SET DEFAULT FOR SEX TO {other-sex} toggles the default
sex.  If the current default is male, {other-sex} will
be "female", and vice-versa.
CHANGE THE DISPLAY opens a screen (Fig. 5d) that allows
you to select a subset of the defined fields for
display and to modify the order in which they appear.
The defined fields are listed on the left of the
screen.  Select (or deselect) fields for display with
the arrow keys and .  They will appear or
disappear from a list on the right.  Only the selected
fields will appear in the spreadsheet and they will
appear in the order they were selected (Fig. 5e).   To
reset the default display, deselect all fields.
 will do this.

MOVE {direction} AFTER DITTO allows you to toggle the
direction of cursor movement after dittoing between
down and right,  {direction} is "right" if the cursor
currently moves down, and vice-versa.   will
implement the change and close the setup screen.  
will just close the screen.

CHANGE THE DITTO CHARACTER TO {other-character} toggles
the ditto character between single and double quote.
The double quote is default, but it requires the shift
key.  If the ditto function is to be used frequently,
it may be more convenient to substitute the single
quote.

MOVE TO LINE (Fig. 5f) allows immediate movement to any
line in the spreadsheet.  Selecting it opens a small
screen into which you enter a line number between 1 and
1000.  You are then returned to the spreadsheet with
the cursor positioned on the indicated line at the same
column.  (The pound sign # is a quick way to do this
directly from the spreadsheet).

IMPORT DATA AS ASCII FILE opens a control screen (Fig. 5g)

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     from which you can name the file to be imported and the
     range of lines within the spreadsheet into which the
     imported data will be entered.   The file to be imported must
     contain data in ASCII format (simple text files)  with fields
     separated by one or more blanks.  (Blanks within fields are
     not permitted.)  The fields themselves are assumed to
     correspond directly to the spreadsheet the way it is
     currently configured (see CHANGE THE DISPLAY above).  When
     •Select source file' is selected, CA will prompt you for a
     search path (Fig. 5h),  and then open a screen depicting all
     files within the indicated path (Fig. 5i). This screen is
     nearly identical to the screens that open when 'Recall' is
     selected from the main menu (Fig. 6).  The only difference
     is that only CA data files will be listed when recalling,
     but every file within the search path will be listed when
     importing.  To select a file from the list, highlight its
     name (with the arrow keys) and .  For an explanation
     of how to change the search path, see Recall (Section 3.1).

     When a file is selected, CA will read the data into the
     corresponding columns of the spreadsheet, beginning at the
     'From1 line and stopping at either the 'To1 line or the end
     of the input file, whichever comes first.  If the file is
     locked (see Section 3.2), CA will note overwritten fields in
     the log but will perform no edits.  Therefore it is critical
     that you make sure the data are legal and the spreadsheet is
     set up correctly.

     EXPORT DATA AS ASCII FILE is the inverse of IMPORT DATA.
     You are again prompted for a filename and two line numbers
     (Fig. 5j), but this time CA will transfer the data in the
     fields defined by the spreadsheet's current configuration to
     the named file.

     IMPORT and EXPORT provide a convenient means to move data
     between CA and other applications.

3. Disk I/O

     •Recall' reads an CA file from disk and makes it the current
file.  'Save' writes the current CA file to disk.   'Import' and
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•Export', being multi-step operations, are too complicated to
execute as a single menu selection.  When selected as main menu
items, 'Import1 and 'Export1 display text screens describing the
procedures.
3.1 Recall

     'Recall' is the means by which existing (previously saved)
CA files are brought back into an active session.  To do this,
'Recall' first identifies the CA files in the current search path
and then presents the resulting list in the main recall screen.

     When 'Recall' is selected, it first offers the option of
changing the current search path (Fig. 5h).  When the search path
has been defined  (that is, when the default has been either
changed or confirmed), 'Recall1 conducts the search and opens the
main screen  (Fig 6).

     The main screen consists of five subwindows.  The first and
by far the largest is the list of files in the current directory
presented alphabetically from left to right in four columns and
as many rows as necessary.  In a column to the right of the file
window are 1) the drive menu, 2) the  'parent directory' button (a
button is just a one item menu), 3) the subdirectory menu, and 4)
the 'cancel1 button.

     The subwindow containing the highlight bar is always the
currently active subwindow.  To move between the subwindows, use
1) CTRL LEFT-ARROW, CTRL RIGHT-ARROW, CTRL PAGE-UP, and CTRL
PAGE-DOWN, or 2) TAB to cycle clockwise.

     When the file list is active, the bar responds to the arrow
keys, and if the list is too long to fit in the window, PAGE-UP
and PAGE-DOWN to scroll.  ENTER will select the currently
highlighted  filename and close the window, returning to the point
of call.

     Of the  remaining subwindows, all but  'cancel1 modify the
                                14

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search path.  Selecting one of the drives redefines the search
path to the root directory of the disk residing on the indicated
drive.

     The 'parent1 button modifies the search path by removing the
last directory level from it.

     Selecting one of the subdirectories modifies the search path
by appending the selected subdirectory to it.

     If the current search path is the root directory, there will
be no parent.  In this case, the highlight bar will skip the
button.  Likewise, there may be no subdirectories within the
current directory.  Again, in this case, the bar will skip the
appropriate button.

      •Cancel1 closes the screen and performs no action.

3.2 Save

     CA incorporates two file attributes, 'locking1 and 'password
protection1.  These attributes can be modified only when files
are saved.

     New files are initially neither locked nor protected.  If
such a file is to be saved, the first menu (Fig. 7a) offers the
options LEAVE UNPROTECTED, PROTECT and CANCEL.  If you select
PROTECT, you will be prompted for the password  (Fig. 7b).  Anyone
wishing to recall this file will have to give the password
exactly as entered here (including capitalization).  If the file
to be saved is already password protected, the first menu (Fig.
7c) will offer related options:  KEEP PASSWORD, CHANGE PASSWORD,
REMOVE PASSWORD, and CANCEL.

     The second menu (Fig. 7d) consists of four items:

     UPDATE THE CURRENT FILE can be selected only if the
     current session was read from disk.  If so, this option
     rewrites the source file, saving any changes made
     during the current session.
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     CREATE A NEW FILE allows you to save the data under a
     new name.  If this item is selected, CA assumes the
     target file does not exist.  If it finds a file with
     the given name, it will prompt you to OVERWRITE or
     CANCEL.

     OVERWRITE AN EXISTING FILE calls a screen identical to
     RECALL SAVED SESSION, except that the selected file
     will be overwritten by the current session.  To save
     disk space, files are compressed before saving.

     CANCEL returns to the main menu.

     Finally, if the current file is not locked, the last menu
(Fig. 7e) will consist of the items: WRITE UNLOCKED, LOCK AND
WRITE, and CANCEL.  If the file is locked, any future additions
or changes will be recorded in its GLP log.  Once a file is
locked, it cannot be unlocked.

3.3 Import and 3.4 Export

      'Import' and 'Export1, being multi-step operations, are too
complicated to execute as single menu selections.  When selected
as main menu items  (as opposed to spreadsheet / F2 menu items),
•Import1 and 'Export1 open text screens describing the respective
procedures.  Leaving 'Import1 and 'Export' as main menu items,
even though they cannot be executed from the main menu,
appropriately reflects their status as high-level operations.

4. Analysis

      •Statistics' conducts specialized statistical analyses on
selected endpoints and presents the results via the screen with
an option to print as well.  The CA software is designed to
analyze an in vivo experiment involving either one or both sexes,
from 1 to 5 scorers, and a maximum of 8 dose groups and 6 sample
times.   'Graph' plots the means of selected response variables.

4.1 Statistics

     When  'Statistics' is chosen from the main menu, CA looks for
                                16

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defined fields representing cell counts suitable for analysis.
If it finds none, CA prints a warning and returns to the main
menu.  If it finds entries for only a single endpoint (e.g.,
mitotic index), CA assumes analysis is to be done on this
endpoint and opens the control screen.  If it finds either the
chromosomal aberration endpoint or the chromosomal aberration and
the mitotic index endpoints selected, it opens an analysis
selection menu (Fig 8a).  Chromosomal aberration data can be
analyzed either as the percentage of cells containing at least
one aberration or as the total number of aberrations.

     The control screen for either of the two chromosomal
aberration statistical tests (Fig. 8b) allows the user to set
certain test parameters and listing options.  The screen consists
of 6 lines: (1) allows you to select the treatment or positive
control data for analysis; (2) sets the significance or alpha
level (default = 0.05); (3) allows you to select one of three
options for including or excluding the high dose from the test
(more on this below).  Because the analysis of the mitotic index
is based on an ANOVA, dropping the high dose is not relevant and
this option is excluded from the control screen (Fig. 8c);  (4)
gives you the option of evaluating scorer differences in addition
to time and sex  (this option is not possible for positive control
data); (5) determines whether the output is to be printed as well
as listed to the screen; and (6) either initiates the statistical
analysis  (GO + ) or returns to the main menu  (CANCEL +
)  .  The current selections for these parameters are
highlighted.

     When the analysis begins, each exposure group  (defined by
sex, treatment and dose) is analyzed for outlier data based on
intragroup standardized residuals.  If any outliers are
identified among the control, treated, or positive control
groups, a screen is opened (Fig. 8d) listing the outliers and
related information .   This screen gives you the options of
printing the outliers, omitting the outliers, retaining them, or
     5 Entering  'g1  at  any point is equivalent  to GO + .
Entering  'c1 is equivalent to CANCEL + .

     6The outliers  listed here  were  created  to demonstrate the
window.  They are not part of the analysis reported in the figures.

                                17

-------
aborting the analysis.

     After the outliers, if any, have been considered, CA
presents (Fig. 8e) the variance inflation factor (a method for
taking into consideration the extent of interanimal variability
present among treatment groups), the alpha level set by the user,
the results of the initial analysis to determine whether the data
can be pooled across sexes and/or across sample times and, if
selected, the results of the scorer analysis.  In the lower box,
the most appropriate model is highlighted, indicating whether the
data should be pooled across sexes, sample time, both or neither.
The user can override the initial selection or return to the main
menu.

     CA uses a statistical approach appropriate to the type of
endpoint selected (i.e., for chromosomal aberration data or for
mitotic index data).  Two statistical procedures for evaluating
the ability of chemicals to induce chromosomal aberrations are
available to the user.  In the first analysis, called chromosomal
aberration (pet. damaged cells), for each animal tested, the
number of cells with at least one aberration  (excluding or
including gaps depending on data input by the user) along with
the total number of cells scored are treated as binomial
observations.  Extrabinomial variability is looked for and
quantified.  Then, for control and treatment group data, ANOVA
procedures are used to test for scorer, sex and sample time
effects while a trend test is used to evaluate treatment effects.
The second analysis, called chromosomal aberration (total
aberrations), treats the total number of aberrations per animal
as Poisson observations (assuming that approximately  (i.e. within
10%) the same number of cells are scored for all animals) and
performs the Poisson counterpart to the binomial analysis
discussed above.  For data on the mitotic index, an ANOVA
analysis is used to evaluate for agent-induced differences in the
frequency of cells in mitosis.  If the statistical analysis is
continued, the next screens (Fig. 8f) present:  (i) the sample
time and sex, the calculated probability value  (p) and the preset
alpha level; and  (ii) summary information on the endpoint
selected, including the group mean and the standard error of the
mean for observations (Note:  In this calculation, each row of
data is treated as a single observation).  The results of a
                                18

-------
comparison of the data at each dose versus the data in the
control group (pairwise significance)  is presented, with
significant values indicated by a '*'.  Pressing any key allows
the user to page through each analysis, eventually being returned
to the main menu.

     For a detailed description of the statistical approach used
in these analyses, see Appendix 2 of the Micronucleus Data
Management and Statistical Analysis Software7.   Generally,  since
the purpose of a chromosomal aberration assay is to determine
whether an agent is capable of inducing a significant increase in
either the frequency of damaged cells or in the total number of
aberrations, the resulting data should be analyzed by a one-
tailed trend test.  One of the assumptions implicit to a
statistical analysis based on a trend test is that the dependent
response increases monotonically with increasing dose.  However,
in a number of biological systems (including in vivo chromosomal
aberration studies), an initial increase in response, followed by
a significant downturn at higher doses, has been detected for a
small number of chemicals.  To avoid the possibility that such
data would result in the improper classification of a test
chemical as being without clastogenic activity, the ability to
formally exclude data at the highest dose only from the trend
test analysis has been included in the statistical package.  Of
course, the user can informally conduct the same type of analysis
by excluding data at specific doses during data entry.  However,
in this case, the analysis for scorer, sex and sample time
effects would not contain the complete data set and may result in
false conclusions about the appropriateness of pooling various
data sets.  If the user decides to formally incorporate the
possibility of excluding from the trend test analysis, at any
future point in time, data at the highest dose, then the preset
alpha level will be adjusted to retain the same overall beta
error rate.  To accomplish this goal, the alpha level set by the
user (default level of 0.05) will be reapportioned between a
primary analysis, which would include all dose data, and a second
analysis, which would exclude data at the highest dose.  The
      Micronucleus Assay Data Management and Analysis System, Data
Management  Systems in Genetic  Toxicology,  Integrated Laboratory
Systems, version 1.4, October,  1990.
                                19

-------
alpha for the primary analysis has been set at 80% alpha (default
of 0.04), while that for the second test has been set at 20%
alpha (default of 0.01).  It would be statistically inappropriate
for the user to first analyze the data set at 100% alpha,  and
then decide to exclude the data at the highest dose from the
statistical analysis.  The exclusion of the highest dose from the
trend test analysis will have no effect on the analysis for
scorer,  sex and sample time effects, which will utilize the total
data set.

     Two methods of statistical analysis are offered for
evaluating chromosomal aberration data, one based on the
frequency of damaged cells and one based on the total number of
chromosomal aberrations among cells.  The former method is the
statistical approach used most commonly.  However, since the
method is insensitive for detecting agents which, for one reason
or another, selectively induce a very small number of heavily
damaged cells only, the latter method was included also in CA.

     For mitotic index data, an ANOVA is used since the frequency
of cells at metaphase, depending on the duration of the
treatment, may be decreased or increased at a particular sample
time, and because the dose response often does not change
monotonically.

4.2 Graph

     This selection instructs CA to plot the means of a response
variable against a classification variable with the option of
stratifying by a third variable (Fig. 9a).  There is also an
option to present this information in the form of a table (Fig.
9b), either along with or instead of the graph.  The response
values are assumed to be the percentage of cells with
aberrations, the frequency of aberrations per cell and the
mitotic index.  The default is the first variable in this list
that is defined for the current session.  The classification
variables are assumed to be 'Dose' and  'Sample Time1.  The
default is  'Dose1.

     The third 'by' variables are limited to  'Sex1 and  'Sample
Time'.  If a 'by1 variable is selected  (Sex is the default), the
                                20

-------
data will be stratified accordingly and presented.
Stratification means two separate means are computed and plotted
for each value of the classification variable, one for each value
of the 'by' variable.

     The control screen consists of four individual submenus
(Fig. 9c).  Three of these present lists of the defined fields in
the spreadsheet and are used to select the independent, the
dependent, and the classification variables respectively.  The
fourth selects the type of output.  The up and down arrow keys
are used to move within a submenu and the left and right arrow
keys to move between them.  As with the statistics setup menus,
 is not necessary except to select the 'GO1  options.  The
graph presents all data listed, regardless of whether the data at
the high dose are excluded from the trend test analysis or
whether any outliers have been detected and excluded from the
statistical analysis.  By manually removing selected data from
the spreadsheet, the user can exclude these data from the graph.
     Use  to make hard copies of the graphs and
tables.

5. Utility

     'List1 produces a hard copy listing through a printer
connected to the serial port.  There are a number of parameters
with which the user can determine the form of the report.  'Sort'
reorders the lines of the spreadsheet (and optionally subsequent
listings) according to the values of any combination of key
fields, either ascending or descending.  'Clear Session' deletes
all data and removes all Set Up fields, in effect starting over.

5.1 List

     CA can generate a hard copy listing of either the experiment
description, the data or both.  A preliminary menu (not shown)
presents this choice.  A control screen (Fig. lOa) governs
several aspects of the list:

     a) The first three lines allow the user to indicate
                                21

-------
     whether the list is to be sorted and,  if so,  in what
     order.  See the Section 5.3 below on sorting the data
     for more information.

     b)  The next group consists of two lines that control
     the range of lines to be printed.  The default is
     •print to the last non-empty line1, but this can be
     overridden on the second line by typing a range of
     lines into the fields.

     c)  Similarly, CA will by default print all fields.  To
     change this, select the second of the next group of
     lines 'Select Subset1.  This will open a window (Fig.
     lOb) in which all the defined fields are listed on the
     left and a blank list to be printed is displayed on the
     right.  Select (or deselect) fields for printing with
     the arrow keys and   (they will appear or
     disappear from the list on the right).  The list on the
     left does not change except for small arrows that
     appear beside the names of the currently selected
     fields.  Press  to return to the control page.

     d)  The next two lines, 'Lines per page' and 'Columns
     per page' govern page length and width.  These should
     be adjusted only if the printer text is set to a
     smaller size.

     e)  The last line contains the commands PRINT which
     begins the printing sequence and CANCEL which returns
     to the main menu.  There is currently no way to stop
     printing short of restarting the equipment.

5.2 Clear Session

     This is an easy way of starting over.   All data and session
configurations are erased.  There is no screen associated with
the selection.  To prevent accidental erasure of unsaved changes,
the user will be prompted by "Are you sure? Yes or No".
                                22

-------
5.3 Sort

     CA can order the rows of the data spreadsheet based on the
values of certain key fields.  The user must specify what the key
fields are, what their priority is to be, and whether the records
should be sorted by ascending or descending values.  The sort
screen (Fig. 11) lists the fields defined for the current session
in a box on the left and the currently defined key fields in a
box on the right.  Initially, the second list is empty.

     Key fields are selected and deselected with the arrow keys
and .  If the records are to be sorted by descending
values (from largest to smallest) use the '-' key instead of
.  Descending keys are marked with a downward pointing
arrow to their left.  To change a currently selected key from
ascending to descending, highlight its name and type '-'.  To
change it from descending to ascending, type '+'.

     The order in which the keys appear in the 'selected keys'
list on the right reflects their priority.  The records will be
sorted on the first key field and ties will be resolved on the
second and so on.  Any ties remaining when the keys have all been
used will remain, but not necessarily in their original order.
The order in which the key fields are defined is critical since
there is no way to change the order of keys once they are
selected.  To insert a key anywhere other than at the end of the
list, it is necessary to deselect then reselect the existing
keys.  Sometimes it is easiest to clear the list and begin again.
 does this.

     When the key fields have been selected, use  to
begin the sort.  In sorting, CA does not physically reorder the
records but rather builds an index that determines a records
apparent location.  To remove the index, select SORT and 
the key fields.

6. Miscellaneous (Misc.)

     'Key Field Search1 produces a report listing either 1) the
values of selected fields for all CA files in the indicated
search path, or 2) the names of all CA files in the indicated

                                23

-------
search path whose values for selected fields match key values
entered here.  'GLP Log1 presents by screen or printer a record
of all accesses to the file and, after locking, all new entries
or changes to existing entries, who made them and when.

6.1 Key Field Search

     A key field file search will either a) list selected
experiment description fields for all CA files in a given search
path, or b) list all files in the current search path whose
values for the selected fields match user defined target values.

     The initial screen (not shown) allows you to select one of
these options.  If you elect to simply list key fields, a screen
will open identical to that used to select fields for listing or
reconfiguring the spreadsheet.  When the fields to list have been
selected, you will be given the chance to redefine the
searchpath.  When the search has been completed, you may elect to
list the results or view them onscreen as with the GLP log
report.

     If, on the other hand, you elect to list the names of files
with matching key field values, selecting a field opens a
subwindow  (Fig. 12a) into which you enter the target string.
When the target string is defined, a subwindow opens (Fig. 12b)
prompting you for a match criteria (e.g. whether the field is to
exactly match the target string or is only to contain the target
string).  The third option, a partial match, is not yet
implemented.

6.2 GLP Log

     To further compliance with good laboratory practices, CA
maintains a log as part of each file recording the time and date
of each access and the ID entered at the beginning of the current
ses-sion.  If a file has been  'locked'  (see Section 3.2), then
this log will record all additional entries and changes to
existing entries.

     The log may be viewed onscreen or printed.
                                24

-------
Sample Data Sets

     To assist the user in becoming familiar with CA, data from
two sample experiments (CATEST01.ILS and CATEST02.ILS) have been
included (Appendix 2).  Both experiments present data in which
male and female mice were treated once with a test chemical, bone
marrow samples collected at 24, 48 and 72 hours after treatment
and the frequency and distribution of chromosomal aberrations and
the mitotic index were determined by two scorers.  In CATEST01,
the chromosomal aberration data includes cells containing 0, 1 or
2 aberrations.  In CATEST02, cells containing multiple
aberrations were detected among the treated mice.  The same
outlier data are included in both data sets.  The statistical
analyses of the chromosomal aberration data are based on the
assumption that excluding the data at the highest dose would
always be considered and that scorer differences would be
evaluated.  Finally, the trend test analysis was conducted  (i)
without pooling data obtained on males and females or at the
three sample times, and (ii) pooling data, where appropriate,
across sexes and sample times.  The corresponding hard copies of
the experimental information, the chromosomal aberration and
mitotic index data and the results of the various statistical
analyses are provided in Appendix 2.  It is suggested that the
user recall the sample test data files, page through the various
screens and invoke each of the subroutines.  It may also be
useful to modify the entries and rerun the menu options.
     We sincerely hope that you will find this program both
useful and friendly.  We welcome any suggestions or comments you
might have.  Please write, call, or FAX to:

                 EPA Software Development  Project
                  Integrated Laboratory Systems
                    Genetic Toxicology Program
                          P.O.  Box 13501
                 Research Triangle Park, NO 27709
                          (919)  544-4589
                                25

-------

— Setup
Endpoints
Optional
Fields



•Data Entry-
Experiment
Description
Spreadsheet



Leave CA

rDisk I/O
Recall
Save
Export

**
—Analysis-
Statistics
Graph




rUtility
List
Clear
session
Sort


4+
— Misc. — n
Key Field
Search
GLP Log

Return to DOS.
    	 Select Endpoints 	
     Chromosomal Aberrations:          No        Yes
     Mitotic Index:                    No        Yes
                         Go
Cancel
                                  Figures  1  &  2
                                     26

-------
   Label 1
 1 Userl	
 2 	
 3 	
 4 	
 5 	
 6 	
 7 	
 8 	
 9 	
10 	
11 	
12 	
13 	
14 	
15 	
       Define up to  fifteen  additional  fields

Label 2   Description
	   (any description here)	
Type
int
                    Define up to  fifteen additional fields
Label 1
1 Userl
2
3
4
5
6
7
8
9
10
11
12
13
14
15

Label 2 Descripti9n
(any description here!


	 Define value type for field [Userl] 	 n

integer (whole number)
real (decimal)
character (string)
date (string)
flag (y/n)
sex (m/f)
aberration (whole number)

Arrow keys move the selector arrow.
RETURN / ENTER to select.

















                                                                            Type
                                                                            int
                                   Figures 3a & 3b
                                      27

-------
EXPERIMENT 	
Record 	
Laboratory 	
Lab Book 	
Date Started 	
Date Completed ....
Slides Coded By ...
Staining Method . . .
Scored By 	
Entered By 	
Entry Date 	
Proofed By 	


CA-TEST 01
121
ILS
III
06/18/90
08/01/90
MP
GIEMSA
CJH
MP
07/25/90
DC


Experiment

Test
Article

Vehicle

Positive
Control

Test
System
Treatment
Main
Menu
TEST ARTICLE 	
Receipt Date 	
CAS # 	
Source / Lot 	
Appearance 	
Purity 	
stability 	
Storage Conditions
Solubility 	
Hazard Information
067889	
04/20/90	
UNKNOWN	
RADIAN 067H3_
ORANGE POWDER.
UNKNOWN	
1 YEAR	
ROOM TEMPERATURE	
INSOLUBLE IN WATER_
TREAT AS CARCINOGEN.
Remarks
Experiment

Test
Article

Vehicle

Positive
Control

Test
System

Treatment

Main
Menu
                                                                 -PgDn-
                             Figures 4a & 4b
                                  28

-------
VEHICLE  	
Source / Lot  	
Purity 	
Stability  	
Storage Conditions
CORN OIL	
SIGMA/13F100
UNKNOWN	
1 YEAR	
ROOM TEMPERATURE
Remarks
Experiment

Test
Article

Vehicle

Positive
Control

Test
System

Treatment

Main
Menu
                                                                 -PgDn-
POSITIVE CONTROL ..
Receipt Date 	
CAS # 	
Source / Lot 	
Appearance 	
Purity 	
Stability 	
Storage Conditions
Solubility 	
Hazard Information
991870	
01/22/90.
UNKNOWN
RADIAN/110BD
WHITE POWDER.
UNKNOWN	
1 YEAR	
ROOM TEMPERATURE	
INSOLUBLE IN WATER.
CARCINOGEN	
Remarks
Experiment

Test
Article

Vehicle

Positive
Control

Test
System

Treatment

Main
Menu
                                                                 -PgDn-
                              Figures  4c  &  4d
                                  29

-------

TEST SYSTEM
Species 	
Strain 	

Received 	
Quarantined From
Until



MOUSE
B6C3F1
TACONIC FARMS
05/01/90
5/01
5/15_

Routine Husbandry Conditions? (y/n) Y 	

Sex
M
F
Age units

Remarks

Age Weight
Fr. To Fr. To
11 	 11 32.0 34.0
11 	 11 	 27.0 29.0
WEEKS Weicrht units GRAM




Experiment

Test
Article

Vehicle

Positive
Control
Test
System

Treatment
Main
Menu


TREATMENT
Date Started 	
Date Completed . . .
Route 	
Volume 	
Doses 	
Dose Units 	
Number Treatments
Treatment Duration
Treatment Date . . .
Treatment Interval
Number Samples . . .
Sample Date 	
Sample Interval . .
Tissue Cell Type


06/18/90
06/21/90
IP
0.4
0, 500, 1000, 2000
MG/KG
1
NA
06/18/90
NA
3
06/19:06/20:06/21
24 HR
BONE MARROW


Experiment
Test
Article

Vehicle

Positive
Control

Test
System

Treatment

Main
Menu

Figures 4e & 4f
    30

-------
Esc -» Main Menu
      Fl - Help
F2 - Setup
       Scorer
   1
   2
   3
   4
   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
    Treat.
    Code
Sex
Dose
Sample
  Time
Number
Cells
chr-tid
gap
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
MP
c m 0 24 25


Assign current column [Scorer]
Set default for SEX to female
Change the display
Move right after ditto
Change the ditto character to single quote ( ' )
Move to line
Import data as ASCII file
Export data as ASCII file
Cancel

t m 1000 24 25
t m 1000 24 25
0

0
0
0
0
0
0
0
0
1
0

0
1
 Animal
  Slide
 Scorer
1054
   A
  MP
                                                            character
Esc -* Main Menu
      Fl - Help    F2 - Setup
1 _
2
3
4
5
6 _
7
8
9
10
1 1
12
13
14 _
15
Animal
Slide
Scorer
Scorer Treat. Sex
Code
MP c m
MP — — 	 	
... MP
MP
MP
MP
MP
MP •
MP
MP
MP
Set Scorer
From row [
» Go «
MP t m
MP t m
MP t m
MP t m
1054
A
MP
Dose
0

to [
1] to row
» Cancel
1000
1000
1000
1000

Sample Number
Time Cells
24 25

DC]
[ 10]
«
24
5
5
5
5
5
5
5
5
5
5
25
24 25
24 25
24 25

chr-tid
gap
0
1
0
0
0
0
0
0
0
0
1
0
0
0
1

                                 Figures 5a & 5b
                                                            character
                                    31

-------
Jsc •* Main Menu Fl •* Help F2 -> Setup
Scorer Treat. Sex Dose Sample Number chr-tid
Code Time Cells gap
1 DC c m 0 24 25 0
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Animal
Slide
Scorer
DC
DC
DC
DC
DC
DC
DC
DC
DC
MP
MP
MP
MP
MP
1054
A
DC
c
c
c
c
t
t
t
t
t
t
t
t
t
t

m
m
m
m
m
m
m
m
m
m
m
m
m
m

0
0
0
0
500
500
500
500
500
1000
1000
1000
1000
1000

24
24
24
24
24
24
24
24
24
24
24
24
24
24

25
25
25
25
25
25
25
25
25
25
25
25
25
25

1
0
0
0
0
0
0
0
0
1
0
0
0
1

                        character
Figure 5c
 32

-------
 -Animal
 -Slide
 -Scorer
  Treat. Code
  Sex
 -Dose
 -Sample Time
  Number Cells
  chr-tid gap
  chr-some gap
  chr-tid break
  chr-some break
  chr-tid exchange
  chr-some exchange
  other aberratn
  CA/cell exc gap
  cells w/ 0 CA
  cells w/ 1 CA
  cells w/ 2 CA
  cells w/ 3 CA
 !•••   i i more I =
Animal
Slide
Scorer
Sample Time
Dose
 [t] [i] [PAGE UP] [PAGE DOWN] [ENTER] [DELETE] [ESCAPE] [Fl = help]
Esc - Main Menu    Fl - Help    F2 - Setup
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Animal
Slide
Scorer
Animal
1054
1055
1056
1057
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1054
A
DC
Slide
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A

Scorer
DC
DC
DC
DC
DC
DC
DC
DC
DC
DC
MP
MP
MP
MP
MP

Sample
Time
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24

Dose
0
0
0
0
0
500
500
500
500
500
1000
1000
1000
1000
1000

                                Figures 5d & 5e
                                                            character
                                     33

-------
Esc - Main Menu    Fl - Help    F2 - Setup
1
2
3
4 	
5
g
7
8
9
10
11
12
13
14
15
Animal
Slide
Scorer
Animal Slide Scorer Sample
Time
1054 A DC 24
1055
1056
1057 	
1058
1059
1060
1061
1062
1063
1064
1065
1066
1067
1068
1054
A
DC
A DC
A DC
24
24
Dose
0
0
0

Go to line:
A DC
A DC
A DC
A DC
A MP
A MP
A MP
A MP
A MP



24
24
24
24
24
24
24
24
24


500
500
500
500
1000
1000
1000
1000
1000

                                                           character
                                    Figure  5f
                                   34

-------
               Select source file
              start with line    1
              stop with line  1000
                 Go
Cancel
C:\CA\testdata\_
                       search path
                   Figures 5g & 5h
                         35

-------
 CATEST01.ILS
 DELETE.ME
 TESTDATA.CA2
Contents of C:\CA\testdata\
CATESTO1.TXT   CATESTO 2.1LS
EXPINFO        OUTFILE.NEW
CATEST02.TXT
TESTDATA.CA1
— Drives  	n
 A  B  C  D  I
                                                   Parent
                                                                          - n
                                                                              I
                                                                Directories —,
                                                               
                                                                   Cancel
Import C:\CA\testdata\ CATESTOl.ILS
     Export data to file:
      C:\CA\testdata\ascii.out
     start with line    1
     stop with line   260
                         Go
                                  Cancel
                                Figures 5i & 5j
                                    36

-------
 CATEST01.ILS
 TESTDATA.CA1
Contents of C:\CA\testdata\
CATEST02.XLS   DELETE.ME
TESTDATA.CA2
EXPINFO
— Drives
 A  B  C  D
                                                                   Parent
                                                                          - n
                                                                             I
                                                                Directories —i
                                                               
                                                                   Cancel
Recall C:\CA\testdata\ CATEST01.ILS
                                    Figure 6
                                    37

-------
         Password protection
Leave unprotected    Protect    Cancel
  Password?
             Figures 7a & 7b
                 38

-------
                     Password protection
Keep password    Change password    Remove password    Cancel
                   Update the current file
                   Create a new file
                   Overwrite an existing file
                   Cancel

           Current file:  C:\CA\testdata\CATEST01.XLS
                         Figures 7c &  7d
                              39

-------
Write unlocked    Lock and write    Cancel
              Write to file
       C:\CA\testdata\CATEST01.ILS
                Figure 7e
                   40

-------
   » Select endpoints for analysis «
Chromosomal aberration (pet. damaged cells)
Chromosomal aberration (total aberrations)
Mitotic index
Cancel
                 Figure 8a
                      41

-------
Analysis Group:
Significance:
Omit the high dose:
Evaluate scorer differences:
Print report:
                    Go
Treatment
    0.050
    Never
    No
    No
Positive Control

Maybe     Yes
Yes
Yes
               Cancel
Analysis Group:
Significance:
Evaluate scorer differences:
Print report:
Treatment
    0.050
    No
    No
                    Go
Positive Control

Yes
Yes
               Cancel
                          Figures  8b  &  8c
                                42

-------
 There are 2 outliers at the .05 significance  level.
Line Dose Tine Sex
1 3 0.0 24.0 m
2 133 0.0 24.0 m
Pet. Mean Pet.
60.000 13.600
80.000 17.600

Omit outliers Keep outliers
Print outliers Cancel
  Endpoint:  Chromosomal  aberration (pet.  damaged cells)
         Variance  inflation factor:
         Alpha:
                1.000
                0.050
         Factor

         Scorer
         Sex
         Time
Deviance

  30.014
   8.226
  30.904
df
24   0.1843
12   0.7673
 8   0.0001
	 Select trends  option	

 Collapse  on     Sex    Time     Both   Neither

                   =  Best choice:  SEX        =
                           Cancel
                             Figures 8d & 8e
                             43

-------
  Time
     P
     alpha
24.00 (Both sexes)   for 067889

        0.000
        0.050
     One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
             Aberrant      Cells    Percent        SEM
     MG/KG      Cells     Scored   Aberrant  (for Obs)
      0.00          7        450     1.5556     0.5729
    500.00         15        500     3.0000     0.6407
   1000.00         28        500     5.6000     0.8897
   2000.00         56        500    11.2000'    1.1462

Hit any key ..
                                                 Pairwise
                                             Significance

                                                   0.0697
                                                   0.0005
                                                   0.0000
               *
               *
  Time =    48.00 (Both sexes)  for 067889

     p         =    0.000
     alpha     =    0.050

     One tailed test (binomial) for chromosomal aberration (pet. damaged cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
6
37
61
81
Hit any key ..
                           Cells    Percent
                          Scored   Aberrant
                             500     1.2000
                             500     7.4000
                             500    12.2000
                             500    16.2000
                                       SEM
                                 (for Obs)
                                    0.5109
                                    1.3067
                                    1.5755
                                    2.1418
    Pairwise
Significance

      0.0000
      0.0000
      0.0000
                                    Figure 8f
                                       44

-------
Average number of aberrations per cell
0.16 -|
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0.00
C
B
C
B

A C
B
A
C
A
BC

) 500 1000 2000
Dose
 Stratified by Sample Time   A = 24.00



 ENTER -» continue
B = 48.00
72.00
                    Average number of aberrations per cell
For sample time =
Dose 0.00
500.00
1000.00
2000.00
24.00
0.084
0.030
0.056
0.112
48.00
0.012
0.074
0.122
0.162
72.00
0.012
0.040
0.086
0.148
                                 Figures 9a & 9b
                                      45

-------
                                Graph Setup
Independent
   Dependent
      By
   Report
 Dose
 Sample Time
CA/cell exc gap
pet. CA positive
MI percent
Sex
Sample Time
Do not stratify
Graph & table
Graph only
Table only
Cancel
                                                             Select to Go
                                  Figure 9c
                                     46

-------
                       Print unsorted list
                       Build new index and print
                       Print with current index
                       Print all lines
                       Print from line [

                       Print all fields
                       Select subset

                       Lines per page   [
                       Columns per page [
                       1] to line [   260]
                       50]
                        7]
                       Print
            Cancel
—Animal
->Slide
-Scorer
 Treat. Code
 Sex
-•Dose
-Sample Time
-Number Cells
 chr-tid gap
-chr-some gap
—chr-tid break
-chr-some break
-chr-tid exchange
-chr-some exchange
-other aberratn
-CA/cell exc gap
 cells w/ 0 CA
 cells w/ 1 CA
 cells w/ 2 CA
 cells w/ 3 CA
       more l ==
Animal
Slide
Scorer
Dose
Sample Time
Number Cells
chr-some gap
chr-tid break
chr-some break
chr-tid exchange
chr-some exchange
other aberratn
CA/cell exc gap
[T] [I] [PAGE UP] [PAGE DOWN] [ENTER] [DELETE] [ESCAPE] [Fl
                               Figures 10 a & lOb
                                         help]
                                     47

-------
-•Animal
-Slide
"Scorer
 Treat. Code
 Sex
-Dose
-Sample Time
 Number Cells
 chr-tid gap
 chr-some gap
 chr-tid break
 chr-some break
 chr-tid exchange
 chr-some exchange
 other aberratn
 CA/cell exc gap
 cells w/ 0 CA
 cells w/ 1 CA
 cells w/ 2 CA
 cells w/ 3 CA
	 I more i =====
TAnimal
TSlide
TScorer
ISample Time
iDose
[T]  [i]  [PAGE UP] [PAGE DOWN] [ENTER] [+] [-] [DELETE] [ESCAPE] [Fl = help]
                                  Figure  11
                                     48

-------
EXPERIMENT
Record
Laboratory
Lab Book
            Enter search string for  Experiment  / EXPERIMENT
    ILS
     Experiment
EXPERIMENT
Record
Laboratory
Lab Book
            Enter search string for  Experiment  / EXPERIMENT

           	 Search string 	
    ILS
             Search string matches the key field
             Search string is included within the key field
             Search string partially matches the key field
             Cancel
     Experiment
                              Figures  12a  &  12b
                                   49

-------
                           APPENDIX 1

        CHROMOSOMAL ABERRATION  ASSAY  SOFTWARE  DEVELOPMENT
                          PANEL MEMBERS

-Dr.  James  Allen
 U.S.  Environmental Protection  Agency

-Dr.  David  H.  Blakey
 Health  and Welfare Canada

-Dr.  Michael Cimino
 U.S.  Environmental Protection  Agency

-Dr.  Russell J.  DuFrain
 Bristol-Myers Co.

-Dr.  Ed  Frome
 Oak  Ridge  National Laboratories

-Dr.  Osamu  Hirai
 Fujisawa Pharmaceutical Co.  Ltd.

-Dr.  Hank E.  Holden
 Pfizer  Inc.

-Dr.  Graham Hook
 UT-Oak  Ridge National Laboratories

-Dr.  Andrew Kligerman
 U.S.  Environmental Protection  Agency

-Dr.  James  MacGregor
 Toxicology Consultants Services,  Inc.

-Dr.  Barry  H.  Margolin
 University of North Carolina

-Dr.  Al  F.  McFee
 Oak  Ridge  National Laboratories

-Dr.  Robert Naismith
 Biofor, Inc.

-Dr.  Charles H.  Nauman
 U.S.  Environmental Protection  Agency

-Dr.  R.  Julian Preston
 Oak  Ridge  National Laboratories

-------
-Dr.  Michael F.  Salamone
 Ministry of the Environment,  Canada

-Dr.  Juan San Sebastian
 Parmakon Research International

-Dr.  Elizabeth S.  Von Halle
 Oak  Ridge National Laboratories

-Dr.  Deiter Wild
 University of Wuerzburg

-Dr.  Llewellyn R.  Williams
 U.S.  Environmental Protection Agency

-------
   APPENDIX 2




SAMPLE TEST DATA

-------
SAMPLE TEST DATA




    CATEST01

-------
C:\CA\CATEST01.ILS listed at          on
EXPERIMENT 	   CA-TEST 01
Record 	   121
Laboratory 	   ILS
Lab Book	   Ill
Date Started 	   06/18/90
Date Completed ....   08/01/90
Slides Coded By ...   MP
Staining Method ...   GIEMSA
Scored By	   CJH
Entered By	   MP
Entry Date 	   07/25/90
Proofed By	   DC
Remarks
TEST ARTICLE 	   067889
Receipt Date 	   04/20/90
CAS # 	   UNKNOWN
Source / Lot	   RADIAN 067H3
Appearance 	   ORANGE POWDER
Purity 	   UNKNOWN
Stability	   1 YEAR
Storage Conditions    ROOM TEMPERATURE
Solubility 	   INSOLUBLE IN WATER
Hazard Information    TREAT AS CARCINOGEN

Remarks 	  	

-------
VEHICLE 	,
Source / Lot	,
Purity 	
Stability 	
Storage Conditions
Remarks
CORN OIL
SIGMA/13F100
UNKNOWN
1 YEAR
ROOM TEMPERATURE
POSITIVE CONTROL ..
Receipt Date 	
CAS f 	
Source / Lot 	
Appearance 	
Purity 	
Stability 	
Storage Conditions
Solubility 	
Hazard Information

Remarks 	
991870
01/22/90
UNKNOWN
RADIAN/110BD
WHITE POWDER
UNKNOWN
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER
CARCINOGEN

-------
TEST SYSTEM
Species
Strain 	
Supplier ...
Received ...
Quarantined
 From
Until
MOUSE
B6C3F1
TACONIC FARMS
05/01/90
5/01
5/15
Routine Husbandry Conditions?  (y/n)   Y
                           Age
                    Sex  Fr.  To
                     M  11   11
                     F  11   11

              Age units WEEKS
                        Weight
                       Fr.  To
                      32.0 34.0
                      27.0 29.0

                      Weight units GRAM
Remarks
TREATMENT
Date Started 	
Date Completed ...
Route 	
Volume 	
Doses 	
Dose Units 	
Number Treatments
Treatment Duration
Treatment Date ...
Treatment Interval
Number Samples ...
Sample Date 	
Sample Interval ..
Tissue Cell Type
        06/18/90
        06/21/90
        IP
        0.4
        0, 500, 1000, 2000
        MG/KG
        1
        NA
        06/18/90
        NA
        3
        06/19;06/20;06/21
        24 HR
Remarks

-------
Animal    Slide     Scorer    Treat.     Sex       Dose      Sample    Nunber    chr-tid   chr-some  chr-tid
                              Code	Time	Cells	gap	 gap       break
1
2
3
4
5
6
7
8
9
10

11
12
13
14
15
16
17
18
19
20

21
22
23
24
25
26
27
28
29
30

31
32
33
34
35
36
37
38
39
40

41
42
43
44
45
46
47
48
49
50


1054|
1055 j
1056 j
1057J
1058 j
1059 j
1060J
1061 j
1062J
1063J
I
1064 1
1065 1
1066 j
1067)
1068 j
1069)
1070 j
1071 1
1072 j
1073 1
I
1074 1
1075 1
1076|
1077|
1078)
1079J
1080)
1081 1
1082 1
1083|
1
1084|
1085 j
1086 j
1087)
1088 j
1089 1
1090 j
1091 1
1092 j
1093 1
1
1094 1
1095J
1096 1
1097|
1098 1
1099|
1100|
1101J
1102 j
1103 j
I
I
A|
A|
A|
A|
Al
A|
Al
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
Al
A|
Al
A|
Al
A|
A|
A|
A|


HP) C
HP | C
HPJ C
HP | C
HP | C
HPJ t
MPJ t
HP | t
NPJ t
MPJ t
I
HP | t
HP | t
MPJ t
HP | t
HPJ t
MPJ t
MPJ t
MPJ t
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HP | t
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HP) C
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HP | t
MP| t
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MP| t
I
MP| t
MP| t
MP| t
MP| t
MP| t
MP| t
MP| t
MP| t
HP | t
HP | t
I
MP| C
MP| C
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m
m
m
m
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m

m
m
m
m
m
m
m
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m
m


0 1
0 1
0 1
o|
o|
500 1
500 1
500 j
500 1
500 1
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1000 1
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1000 j
1000 j
2000 j
2000 j
2000 1
2000 1
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0|
0|
0|
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500 1
500 1
500 1
500 1
500 1
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1000 1
1000 j
1000 j
1000 j
1000 j
2000 1
2000 1
2000 1
2000 1
2000)
I
o|
o|
o|
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"I
500 1
500 1
500 1
500 1
500 1
I
I
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
I
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
I
48|
48|
48 1
48 1
48 1
48 1
48|
48 1
48 1
48 1
I
48 1
48|
48 1
48|
48 1
48 1
48|
48 1
48 1
48 1
I
n\
n\
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72|
72|
72|
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72!
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25 1
25 1
25 1
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25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
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25|
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
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25 1
25 1
25 1
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25 1
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25 1
25 1
25 1
25 1
25 1
25 1
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'5 1
25 1
25 1
I
I
0|
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0|
0|
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Q I
Q I
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11
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0|
0|
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0|
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o|
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o|
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0|
0|
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o|
I
0|
0|
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o|
0|
0|
o|
o|
0|
0|
I
0|
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o|
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0|
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0|
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°l
0|
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0|
0|
15|
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11
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0|
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11
11
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11
2|
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3|
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3|
2|
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1
0|
1|
0|
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2|
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1|
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6 1
3|
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7|
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0|
11
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0|
11
11
I
I

-------

1
2
3
4
5
6
7
8
9
10

11
12
13
U
15
16
17
18
19
20

21
22
23
24
25
26
27
28
29
30

31
32
33
34
35
36
37
38
39
40

41
42
43
44
45
46
47
-8
49
50


chr-som
break
o|
o|
o|
o|
0|
o|
°l
0|
o|
o|
I
0|
0|
o|
"I
o|
o|
o|
°l
o|
0|
I
o|
o|
°l
o|
o|
o|
o|
o|
o|
o|
I
0|
o|
o|
o|
o|
o|
o|
0|
o|
o|
I
°l
o|
°l
o|
o|
o|
o|
°l
o|
o|
I
I
chr-tid
exchange
o|
o|
°l
o|
°l
°l
0|
°l
"I
o|
I
°l
0|
"I
o|
o|
°l
o|
o|
o|
"I
I
o|
0|
"I
°l
o|
o|
0|
o|
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I
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o|
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I
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0|
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chr-sotne
exchange
°l
0|
o|
o|
°l
0|
0|
0|
o|
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I
«l
o|
0|
°l
0|
0|
0|
0|
0|
o|
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0|
°l
"I
«l
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0|
0|
0|
0|
°l
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0|
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»l
°l
0|
o|
0|
0|
0|
I
o|
°l
°l
0|
0|
o|
0|
0|
0|
°l
I
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other
sberratn
0|
0|
0|
0|
°l
"I
«l
o|
0|
0|
I
0|
0|
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°l
o|
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0|
0|
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0|
0|
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0|
0|
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0|
0|
°l
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0|
0|
0|
0|
0|
Q|
0|
°l
o|


CA/cell
exc gap
o|
o|
0.600 |
0.040J
0.040J
o|
"I
o|
0.040]
0.040)
I
0.040)
0.080 |
0.040J
o|
0.040|
0.120 j
0.160 |
0.120 j
0.080 j
0.040 |
I
o|
0.040|
«l
0|
0.040|
0.040 j
0.080 |
0|
0.040|
0.040 |
I
0.120|
0.120 1
0.080 |
0.200 j
o|
0.240J
0.120)
0.080)
0.280 j
0.200)
I
0|
0|
o|
C.040)
0|
0.040)
o|
0|
0.040)
0.040|
I
I
cells w/
0 CA
25)
25)
10)
24)
24 1
25)
25)
25 1
24 1
24 1
I
24)
S|
24)
25)
24)
22)
21 1
22)
23)
24)
I
25)
24)
25)
25)
24)
24)
23)
25)
, 24)
24)
I
22)
B |
22 1
20 1
25)
19)
22|
23)
19)
20)
I
25 1
25 1
25 1
24)
25)
24)
25)
25)
24 1
24 1
I
I
cells w/
1 CA
0)
°l
15)
1|
11
o|
0|
0|
1|
1|
1
1|
2|
M
0)
M
3|
«l
3)
2)
1)
I
0|
11
o|
0|
11
11
2)
0)
11
1|
1
3)
2|
3|
5|
"1
*l
3|
2|
5|
5|
1
0|
"1
0|
M
0|
11
0|
0|
M
'I
'I
I
cells w/
2 CA
°l
o|
0|
o|
0|
0)
0|
°l
o|
o|
I
o|
o|
"I
«l
o|
0|
°l
"I
0|
0|
I
o|
»l
0|
0|
o|
o|
o|
0)
0|
0|
I
o|
0|
o|
o|
0|
0|
"I
0|
M
o|
I
o|
0|
o|
<>l
»l
0|
o|
0|
°l
0|
I
!
cells w/
3 CA
0|
0|
°l
0|
0|
»l
o|
°l
«l
o|
I
0|
0|
o|

-------

1
2
3
4
5
6
7
8
9
10

11
12
13
14
15
16
17
18
19
20

21
22
23
24
25
26
27
28
29
30

31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50


cells u/
6 CA
0|
o|
o|
o|
o|
0|
0|
°l
0|
0|
I
°l
o|
o|
0|
o|
o|
0|
0|
o|
0|
I
o|
o|
o|
0|
0|
o|
0|
o|
»l
o|
I
o|
0|
0|
o|
0|
0|
0|
0|
o|
0|
1
1
01
0|
o|
o|
o|
0|
o|
0|
0|
o|
1
1
cells w/
7 CA
«l
o|
o|
o|
°l
o|
0|
"I
o|
o|
1
o|
0|
o|
°l
o|
o|
"1
o|
o|
o|
1
0|
0|
o|
0|
°l
°l
0|
0|
0|
o|
1
°l
°l
0|
o|
0|
0|
°l
°l
o|
o|
1
1
o|
0|
»l
0|
0|
o|
°l
°l
0|
o|
1
1
cells u/
8 CA
o|
0|
°l
o|
o|
0|
°l
«l
o|
o|
1
0|
o|
PI
0|
o|
o|
o|
o|
o|
o|
1
«l
o|
o|
o|
o|
o|
0|
0|
0|
0|
1
o|
o|
o|
o|
0|
0|
0|
°l
0|
o|
1
1
o|
o|
«l
0|
"I
'-\
°l
o|
o|
0|
1
1
cells M/
9 CA
0|
0|
°l
0|
»l
0|
o|
°l
o|
0|
1
»l
"1
°l
»l
o|
«l
o|
0|
°l
0|
1
0|
°l
0|
«l
0|
0|
0|
°l
0|
o|
1
0|
o|
"I
o|
0|
0|
0|
o|
°l
0|
1
1
0|
0|
°l
o|
0|
°l
o|
°l
0|
0|
1
1
cells w/
10* CA
o|
0|
0|
0|
0|
0|
o|
0|
"1
0|
1
o|
o|
o|
0|
0|
0|
o|
o|
o|
o|
1
0|
0|
o|
«l
0|
0|
o|
0|
°l
0|
1
°l
0|
0|
o|
0|
0|
o|
0|
0|
c|
1
1
o|
0|
°l
°l
0|
o|
3|
°l
0|
0|
1
1
pet. CA
positive
0.0|
0.0|
60. OJ
4.0|
4.0|
o.oj
o.oj
o.oj
4.0|
4.0|
1
4.0|
8.0|
4.0|
0.0|
4.0|
12. 0|
16. OJ
12. OJ
8.0J
4.0|
I
0.0|
4.0|
0.0|
o.oj
4.0|
4.0)
8.0J
o.oj
4.0|
4.0|
I
12.0|
8.0J
12.0J
20. OJ
o.oj
24. OJ
12. 0|
8.0J
24. OJ
20. OJ
i
1
0.0|
0.0|
0.0|
4.0|
o.ol
4.0|
0.0|
o.oj
4.0|
4.0|
1
1
HI
* cells
500)
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 1
500 j
500 j
1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 1
i
1
500 1
500 j
500 j
500 1
500 1
500 1
500 1
500 j
500 j
500 j
1
1
MI
# meta
«|
«l
21 1
10|
»l
25 1
33 1
14|
18|
«l
I
16|
25 1
24 1
21 1
«l
16|
25 1
14|
25 1
24 1
I
25|
28|
32 1
18|
19|
21 1
24 1
18|
17|
19|
I
15|
14 1
«|
15|
18|
10|
»l
5|
•I
7\
i
1
25 1
32 1
18|
12|
15|
18|
19|
21 1
22 1
«|


MI
percent
3.00J
2.40)
4.20J
2.00J
1.80J
5.00J
6.60J
2. 80 j
3.60J
3.00J
I
3.20|
5.00J
4. 80 j
4.20J
3. 00 j
3. 20 j
5.00J
2.80J
5. 00 1
4. 80 1
I
5.00|
5. 60 j
6. 40 j
3.60J
3. 80 1
4. 20 1
4.80J
3. 60 1
3.40J
3. 80 1
I
3.00|
2.80J
2.60J
3. 00 1
3.60J
2.00J
1.80 1
1.00 j
1.60 j
1.40J
i
1
S.OOj
6.40J
3.60|
2.40J
3.00J
3.60J
3. 80 1
4.20J
4.40J
3.00|
1
1

-------

51
52
53
54
55
56
57
58
59
60

61
62
63
64
65
66
67
68
69
70

71
72
73
74
75
76
77
78
79
80

81
82
83
84
85
86
87
88
89
90

91
92
93
94
95
96
97
98
99
100


Animal Slide
1104)
1105 1
1106 1
1107J
1108 1
1109)
1110J
1111J
1112J
1113J
I
1114|
1115J
1116J
1117J
1118J
1119J
1120 j
1121 1
1122 j
1123)
I
1124|
1125|
1126|
1127|
1128|
1129|
1130 1
1131 j
1132 j
1133 j
I
1134|
1135|
1136|
1137|
1138|
1139|
1140|
1141|
1142|
1143|
1
1144|
1145|
1146|
1147J
1148|
1149|
1150|
1151|
1152J
1153|
1
1

*l
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
*l
A|
A|
A|
A|
A|
*l
A|
*l
*l
1
1
Scorer
MP|
MP|
MP|
HP|
MP|
MP|
MP|
MP|
MP|
MP|
1
MP|
MP|
MP|
HP|
MP|
HP)
MP|
HP|
HP|
HP|
1
MP|
HP|
HP|
*P|
MP|
NP|
MP|
HP|
MP|
HP|
1
HP|
MP|
HP|
"I
HP|
MP|
MP|
MP|
HP|
MP|
1
MP|
MP|
MP|
MP|
HP,
MP|
MP|
MP|
MP|
«P|
1
1
Treat. Sex
Code
t|
t
t|
t|
t
t|
t|
t|
t
t

e|
c|
c
c|
c
tl
tl
tl
tl
tl
1
t
t
tl
tl
tl
tl
tl
tl
tl
tl

c
e
c
c
e
t
t|
t|
t|
t
1
t|
t
t
t|
t|
t|
t|
t|
t|
t



"1
"I
•1
H
•1
•1
•H
•1
m|
•1
1
'1
'1
'1
«l
*l
*l
'1
f|
'I
*l
1
*l
*l
*l
'1

-------

51
52
53
54
55
56
57
58
59
60

61
62
63
64
65
66
67
68
69
70

71
72
73
74
75
76
77
78
79
80

81
82
83
84
85
86
87
88
89
90

91
92
93
94
95
96
97
93
99
100

chr-8l
0|
0|
i
I
cells w/
5 CA
0|
«l
"I
0|
0|
o|
0|
»l
o|
o|
I
0|
°l
o|
o|
o|
o|
0|
»l
«l
«l
I
o|
0|
o|
0|
o|
o|
o|
0|
0|
o|
I
°l
"I
0|
»l
0|
«l
"I
0|
o|
o|
I
o|
o|
o|
0|
0|
6i
0|
«l
°l
0|
I
I

-------
cell* w/ cells w/ c«lU w/ cell* w/ cell* u/ pet. CA
6 CA 7 CA 8 CA 9 CA 10+ CA positive #
51 0|
52 OJ
53 OJ
54 0|
55 OJ
56 0|
57 OJ
58 0|
59 OJ
60 0|
1
1
61 0|
62 0|
63 OJ
64 OJ
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90

91
92
93
94
95
96
97
98
99
100

o|
o|
o|
»l
o|
o|
1
1
0|
«l
o|
0|
0|
o|
»l
°l
0|
o|
1
1
0|
0|
o|
°l
o|
0|
o|
o|
0|
0|
1
0|
0|
"1
0|
0|
°l
°l
0|
l
°l
0|
0|
«l
0|
0|
01
1
0|
o|
0|
o|
0|
0|
0|
0|
o|
o|
1
1
1

-------

101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120

121
122
123
124
125
126
127
128
129
130

131
132
133
134
135
136
137
138
139
140

141
142
143
144
145
146
147
148
149
150


Animal
1154|
1155J
1156J
1157J
1158 j
1159J
1160)
1161)
1162J
1163 j
1
1
1164|
1165 1
1166 j
1167 j
1168 j
1169 1
1170J
1171)
1172|
1173|
1
1174|
1175|
1176|
1177|
1178|
1179|
1180J
1181 j
1182)
1183 j
I
1054 1
1055 1
1056)
1057 j
1058 j
1059]
1060J
1041 1
1062J
1063 j
I
1064 1
1065 1
1066 1
1067 1
1068 1
1069 1
1070|
1071 1
1072 1
1073 1
I
I
Slide
A|
A|
A|
A|
A|
*l
A|
A|
A|
A|
1
1
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
1
A|
A|
At
A|
A|
A|
A|
A
A
A

• B
B
B
B
B|
8|
8|
B|
3|
B|
1
B
B
B
B|
B|
B|
B|
B|
5
B|


Scorer
MP|
MP|
MP|
MP|
MP|
MP|
HP|
MP|
MP|
HP|
|
HP|
MP|
MP|
HP|
HP|
HP|
MP|
MP|
NP|
HP|
1
HP|
NP|
MP|
MP|
HP|
MP|
HP|
HP|
HP|
MP|
1
BN|
BN|
BN|
BN|
BN|
BN|
BN|
8M|
BK|
BN|
1
BN|
B*|
BN|
BN|
BM|
BN|
BN|
BN|
BN[
BM|
1
1
Treat. Sex
Code
c
c
c
c
c
t
tl
tl
tl
tl
1
1
tl
tl
tl
tl
tl
tl
tl
tl
tl
tl
1
Pi
Pi
Pi
Pi
Pi
Pi
p
Pi
Pi
Pi
1
c|
c|
c|
c|
c
t
t
tl
t
t

tl
tl
tl
tl
tl
tl
tl
tl

-------

101
102
103
104
105
106
107
108
109
110

111
112
113
114
115
116
117
118
119
120

121
122
123
124
125
126
127
128
129
130

131
132
133
134
135
136
137
138
139
140

141
142
143
144
145
146
147
148
149
150

chr-some
break
0|
0|
0|
0|
"I
"I
0|
0|
°l
0|
1
0|
0|
0|
0|
0|
0|
o|
0|
1|
o|
1
1|
0|
o|
11
°l
o|
Ol
o|
o|
11
1
0|
0|
0|
o|
01
0|
o|
o|
o|
0|
1
o|
0|
o|
0|
0|
11
o|
0|
i|
o|
I
I
ehr-tid
exchange
0|
0|
o|
0|
o|
0|
0|
0|
0|
0|
I
o|
o|
0|
o|
o|
0|
0|
0|
0|
o|
I
0|
0|
0|
0|
0|
11
*l
0|
2|
0|
1
0|
oi
o|
0|
0|
0|
0|
0|
o|
0|
1
0|
0|
0|
0|
0|
11
o|
o|
o|
o|
1
1
chr-some
exchange
o|
o|
01
0|
0|
0|
0|
o|
o|
o|
i
o|
0|
0|
o|
01
o|
o|
o|
0|
0|
1
0|
"1
0|
0|
0|
0|
0|
0|
o|
o|
1
0|
0|
0|
o|
0|
0|
0|
0|
0|
o|
1
0|
0|
0|
o|
0|
0|
0|
0|
0|
o|
1
1
other
aberratn
0|
»l
0|
0|
0|
0|
0|
0|
0|
0|
1
0|
01
o|
0|
0|
o|
o|
0|
o|
o|
1
0|
0|
0|
o|
0|
0|
0|
o|
0|
o|
1
0|
0|
0|
0|
0|
0|
0|
0|
0|
0|
1
0|
o!
0|
0|
o|
0|
0|
0|
o|
0|
1
1
CA/cell
exc gap
0.040)
0|
0|
0.040J
0|
0.040|
0.040J
0|
0.080|
0.080)
1
0.120|
0.080 j
0.040J
0.080 j
0.040 |
0.200|
0.040 |
0.120 j
0.080)
0.200 |
I
0.320 |
0.240)
0.160)
0.440 j
0.400 |
0.400 |
0.280 |
0.160)
0.240)
0.280)
1
0|
o|
0.800)
0.080 j
o|
0.040)
0.040)
o|
0.080)
0.080)
1
0.080)
0.080)
0|
0.160)
0.080 |
o.;?o|
0.120)
0.080)
0.200!
0.030)
I
I
cells w/
0 CA
24)
25)
25)
24)
25)
24)
24)
25)
23)
23)
I
22)
23)
24)
23)
24)
20 1
24)
22)
23)
20)
I
"I
19)
21)
15)
17)
16)
18)
21 1
19|
19)
1
25)
25)
5)
23|
25 1
24)
2*1
25)
23)
23)
1
23)
23|
25)
21)
23)
19)
22)
23)
20 1
23|
I
I
cells w/
1 CA
1|
o|
o|
11
0|
1)
1)
0|
2|
2|
I
3)
2|
M
2|
1|
5|
1|
3|
2)
5)
I
•I
«l
*l
'I
n
•I
7|
*l
«l
5|
I
0|
0|
20)
2)
0|
1|
'I
o|
2|
2|
1
2|
21
0|
*l
2|
5|
3)
2)
5|
2|
1
1
cells u/
2 CA
o|
o|
0|
o|
' o|
o|
0|
0|
0|
o|
1
0|
o|
0|
0|
0|
0|
0|
0|
0|
0|
1
0|
0)
"1
M
0|
11
0|
0|
0|
i|
1
o|
0|
o|
0|
0|
0|
01
o|
*l
0|
1
0|
0|
0|
0|
0|
M
0|
o|
0|
o|
I
I
cells M/
3 CA
0|
0)
0|
0|
o|
o|
0|
0|
0|
"I
I
o|
o|
0|
0|
0|
o|
0|
0|
0|
o|
I
0|
0|
0|
o|
1)
o|
o|
0|
0|
0|
I
o|
0|
o|
o|
0|
0|
0|
0|
o|
»l
I
0|
0|
0|
0|
0|
o|
0|
o|
0|
0|
I
I
cells M/
4 CA
0|
0|
0|
0)
0|
0|
o|
0|
0)
0|
I
0|
0|
"I
0|
0|
0|
0)
0|
0|
o|
I
0|
0|
o|
0|
0|
0|
0|
o|
0|
o|
I
0|
o|
0|
0|
o|
0|
0|
0|
o|
0|
I
0|
0|
o|
o|
0|
o|
o|
0|
0|
0|
I
I
cells w/
5 CA
0|
o|
0|
0|
0|
0|
0|
0|
0|
0|
I
0|
o|
0|
0|
0|
0|
0|
0|
0|
0|
I
0|
0|
0|
0|
"I
0|
0|
0|
0|
0|
I
0|
0|
0|
o|
0|
0|
0|
0|
0|
0|
I
o|
0|
0|
0|
ol
c|
0|
0|
0|
0|
I
I
11

-------

101
102
103
104
105
106
107
108
109
110

111
112
113
114
115
116
117
118
119
120

121
122
123
124
125
126
127
128
129
130

131
132
133
134
135
136
137
138
139
140

141
142
143
144
145
•i6
U7
148
149
150


celts w/
6 CA
°l
"I
o|
o|
°l
°l
0|
0|
o|
o|
I
o|
"I
o|
o|
o|
°l
0|
0|
0|
0|
I
o|
o|
o|
°l
o|
o|
°l
"I
o|
0|
I
0|
o|
0|
°l
o|
°l
o|
o|
"I
o|
I
°l
0|
o|
°l
°l
°l
°l
°l
0|
°l
I
I
cells u/
7 CA
0|
0|
o|
0|
0|
0|
o|
o|
o|
o|
I
o|
0|
"I
°l
0|
0|
°l
0|
0|
°l
I
o|
°l
o|
0|
o|
°l
0|
°l
o|
°l
I
o|
°l
0|
o|
0|
0|
o|
°l
o|
°l
I
o|
0|
°l
°l
°l
°l
o|
o|
o|
o|
I
I
cells w/
8 CA
o|
°l
0|
o|
0|
o|
°l
o|
o|
°l
I
o|
0|
o|
°l
°l
o|
°l
o|
°l
o|
I
o|
o|
°l
0|
0|
°l
o|
o|
o|
°l
I
°l
°l
°l
o|
°l
0|
o|
o|
o|
0|
I
o|
o|
°l
°l
o|
°l
o|
0|
°l
0|
I
I
cells w/
9 CA
0|
"I
"I
o|
0|
0|
°l
0|
«l
0|
I
°l
o|
0|
0|
o|
0|
0|
o|
o|
o|
I
o|
o|
"I
o|
o|
o|
"I
°l
"I
o|
I
o|
o|
0|
o|
o|
o|
o|
o|
°l
0|
I
0|
0|
o|
°l
°l
o|
o|
0|
o|
0|
I
I
cells w/
10+ CA
01
°l
f|
0|
0|
o|
o|
o|
o|
0|

0|
°l
0|
o|
0|
0|
o|
0|
o|
"1
1
o|
0|
0|
°l
o|
°l
o|
o|
o|
o|
1
°l
0|
0|
0|
0|
o|
o|
°l
°l
01

o|
o|
o|
°l
o|
0|
01
o|
o|
01


pet. CA
positive
4.0|
0.0|
0.0|
4.0|
0.0|
4.0|
4.0|
0.0|
s.oj
8.0J
1
12.0|
8.0J
4.0|
8.0|
4.0|
20. 0|
4.0|
12. 0|
8.0J
20. OJ
I
32. 0|
24. 0|
16. OJ
40.0)
32. OJ
36. 0|
28. OJ
16. OJ
24. OJ
24. OJ
I
0.0|
o.oj
80. OJ
8.0J
o.oj
4.0J
4.0|
0.0|
8.0|
8.0|
I
8.0|
8.0|
0.0|
16. OJ
8.0|
24. 0|
12. 0|
8.0|
20. OJ
8.0J
I
I
MI
# cells
500 1
500 1
500 j
500 j
500 j
500 j
500 j
500 1
500 1
500 j
I
500)
500 j
500 j
500 j
500 1
500 1
500 j
500 j
500 j
500 1
I
500 1
500 j
500 j
500 1
500 j
500 j
500 1
500 j
500 j
500 j
I
500 1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 1
500 j
500 1
500 1
500 1
500 1
500 j
500 1
500 1
I
I
MI
# meta
12|
15|
14|
25 1
24 1
21 1
25 1
15|
16|
25 1
I
28|
24 1
25 1
26|
28 1
24 1
25 1
26|
32 1
19|
I
15|
32 1
15|
28|
24 1
23|
25 1
25 1
21 1
25 1
1
25|
24 1
32 1
33 1
15|
15|
25 1
24 1
26 1
23|
I
25 1
14|
25 1
23|
25 1
32 1
18|
17|
16|
15|
1
1
MI
percent
2.40|
3.00|
2.80J
5.00J
4.80J
4.20J
5.00J
3. 00 j
3.20J
5.00J
1
5.60|
4.80J
s.ooj
5.20J
5. 60 j
4. 80 j
5.00J
5.20J
6.40J
3. 80 j
1
3.00|
6.40J
3.00J
S.60J
4.80J
4.60J
s.ooj
5.00J
4.20J
5.00J
1
5.00)
4.80J
6. 40 j
6.60J
3. 00 j
3. 00 j
s.ooj
4.80|
5.20J
4.60J
1
s.ooj
2.80J
5.00J
4.60J
S.00|
6.40|
3.60J
3.40|
3.20J
3.00|
1
1
12

-------

151
152
153
154
155
156
157
158
159
160

161
162
163
164
165
166
167
168
169
170

171
172
173
174
175
176
177
178
179
180

181
182
183
184
185
186
187
188
189
190

191
192
193
194
195
196
197
198
199
200


Animal Slide
1074 1
1075 1
1076 j
1077|
1078 1
1079J
1080 1
1081 1
1082 j
1083 1
I
1084)
1085 1
1086 j
1087 1
1088 j
1089 j
1090 1
1091 1
1092)
1093 j
I
1094 1
1095J
1096 1
1097J
1098 1
1099J
1100 1
1101 j
1102|
1103|
1
1104|
1105|
1106|
1107)
1108|
1109|
1110J
1111J
1112|
1113|
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1114)
1115J
1116|
1117)
1118J
1119|
1120)
1121 j
1122J
1123)
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Bl
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Scorer
BN|
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BX|
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Treat. Sex
Code
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500 j
500 j
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500 1
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1000 1
1000 j
1000 j
1000 1
1000|
2000 j
2000 j
2000 j
2000 1
2000 1
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500 1
500 1
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1000|
1000 j
1000 j
1000 1
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2000)
2000 j
2000 1
2000 1
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0|
o|
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°l
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500 1
500 1
500 1
500 1
500 1
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Sample
Time
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
I
48|
48|
48 1
48 1
48 1
48 1
48|
48 1
48 1
48|
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72|
72J
72|
72|
72|
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72 1
72 1
72 1
72 1
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72 1
72 1
72 1
72 1
72 1
72 1
72 1
72 1
72 1
72 1
I
24 1
24 1
24 1
2* I
24 1
24 1
24 1
24 1
24 1
24 1
I
I
Nutfcer
Cells
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
2S|
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25|
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
I
chr-tid
gap
0|
o|
0|
1|
"I
"I
o|
0|
3|
o|
I
"I
4|
0 1
Q 1
3|
Q 1
3 j
0|
°l
4I
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°l
o|
°l
°l
3|
oj
0|
o|
0|
°l
I
0|
°l
0|
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2|
o|
o|
°l
°l
0|
I
2|
0|
Q I
0 I
0 1
o|
Q I
Q I
0 1
3|
I
I
chr-some chr-tid
gap break
0| 0
0| 0
0| 1
0| 0
OJ 0
0| 3
0| 4
0| 5
0| 0
0| 2
1
0| 4
0| 4
0| 8
0| 3
0| 0
0| 8
0| 2
0| 7
0| 3
0| 3
1
0| 0
0| 0
0| 0
0| 1
0| 0
0| 1
0| 1
0| 2
0| 0
OJ 2
I
0| 2
0| 3
0| 4
0| 0
0| 2
0| 1
0| 3
0| 8
0| 5
0| 5
I
0| 0
0| 0
0 1 1
0 1 1
0| 1
0| 0
0| 0
0| 1
0| 2
0| 1
1
1
13

-------

151
152
153
154
155
156
157
158
159
160

161
162
163
164
165
166
167
168
169
170

171
172
173
174
175
176
177
178
179
180

181
182
183
184
185
186
187
188
1S9
190

191
192
193
194
195
196
197
198
199
200
chr-some
break
"I
o|
0|
0|
0|
0|
0|
o|
o|
o|
I
0|
1|
1|
0|
°l
1|
0|
0|
o|
°l
1
o|
o|
o|
"I
0|
o|
°l
o|
0|
0|
1
0|
o|
2|
"1
0|
"1
0|
o|
1|
°l
I
o|
»l
°l
0|
0|
0|
0|
o|
°l
0|
I
I
chr-tid
exchange
"I
0|
°l
o|
o|
0|
o|
11
0|
0|
I
"I
«l
11
o|
3|
0|
o|
1|
3|
o|
1
o|
o|
°l
o|
o|
°l
0|
°l
0|
0|
1
o|
o|
11
o|
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0|
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"1
°l
0|
1
0|
0|
0|
o|
0|
°l
0|
0|
o|
0|
1
1
chr-some
exchange
0|
o|
o|
o|
0|
o|
0|
°l
0|
o|
1
o|
o|
o|
o|
o|
0|
0|
°l
o|
0|
1
0|
°l
0|
°l
0|
o|
0|
o|
°l
0|
1
0|
0|
0|
°l
o|
0|
0|
o|
°l
o|
1
o|
0|
0|
0|
o|
°l
o|
°l
0|
0|
1
1
other
aberratn
o|
0|
o|
o|
"I
o|
°l
"1
0|
o|
1
0|
o|
o|
o|
0|
o|
o|
"1
o|
o|
1
0|
o|
°l
o|
o|
o|
°l
o|
0|
o|
1
«l
o|
0|
°l
o|
o|
0|
°l
°l
°l
1
"I
°l
0|
0|
o|
°l
0|
°l
3|
°l
1
1
CA/cell
exc gap
°l
°l
0.040J
°l
0|
0.120J
0.160 |
0.240 j
o|
0.080)
I
0.160]
0.200 1
0.400 j
0.120 j
0.120 j
0.360 j
0.080 |
0.320 j
0.240J
0.120 j
I
0|
°l
o|
0.040)
°l
0.040 |
0.040 j
0.080 j
°l
o.osoj
I
0.080)
0.120 j
0.280]
°l
0.080]
0.040]
0.120]
0.320 1
0.240]
0.200 |
I
°l
0|
0.040]
0.040]
0.040]
0|
0|
0.040]
0.080]
0.040|
I
I
cells w/
0 CA
25
25
24 1
25
25 1
22 1
21 1
19|
25 1
23

21 1
20 1
17|
22|
22 1
16]
23 1
18|
21 1
22 1
I
» I
25 1
25 1
24 1
25 1
24 1
24 1
23 1
25 1
23|
I
23 1
22|
19|
25 1
23 1
24 1
22 1
18|
21
20 1

25 1
25
24 1
24 1
24 1
25 1
25
24 1
23 1
24 1
cells u/
1 CA
°l
"I
1|
o|
"I
3|
*l
*l
°l
2|

*l
*l
n
3|
3|
'I
2|
«l
3|
3|
I
0|
°l
o|
11
0|
11
11
2|
"I
2|
I
2|
3|
5|
"I
2|
1|
3|
«l
2|
S|

0|
0|
1|
1
1|
°l
0|
11
2|
1|
cells u/
2 CA
o|
0|
0|
0|
"I
0|
o|
"I
o|
0|
I
0|
1|
0|
0|
o|
o|
o|
11
o|
o|
I
o|
0|
o|
0|
o|
"I
"I
"I
0|
0|
I
°l
0|
11
0|
o|
o|
o|
11
2|
0|
1
o|
0|
01
»l
°l
"I
0|
0|
0|
°l
1
1
cells w/
3 CA
0|
0|
0|
0|
0|
0|
0|
»l
o|
0|
1
o|
o|
11
0|
0|
0|
0|
"I
11
o|
1
0|
o|
0|
«l
o|
°l
»l
o|
"1
o|
1
°l
0|
o|
o|
o|
o|
o|
0|
o|
0|
1
°l
01
°l
0|
o|
0|
0|
0|
»l
°l
1
1
cells u/
4 CA
°l
o|
»l
0|
0|
o|
0|
o|
0|
o|
1
»l
0|
0|
o|
»l
o|
o|
"1
o|
«l
1
0|
o|
«l
0|
o|
0|
0|
"I
0|
0|
1
°l
0|
o|
0|
»l
°l
0|
0|
0|
o|
1
°l
0|
o|
o|
0|
o|
°l
0|
o|
0|
1
1
cells w/
5 CA
o|
o|
0|
0|
o|
0|
»l
»l
0|
0|
1
»l
0|
0|
o|
"I
0|
»l
o|
o|
o|
1
0|
0|
0|
o|
"1
"I
0|
0|
"I
o|
1
0|
0|
o|
o|
0|
«l
o|
o|
0|
0|
1
o|
0|
0|
o|
0|
0|
0|
0|
o|
0|
1
1
14

-------

151
152
153
154
155
156
157
158
159
160

161
162
163
164
165
166
167
168
169
170

171
172
173
174
175
176
177
178
179
180

181
182
183
184
185
186
187
188
189
190

191
192
193
194
195
196
197
198
199
200


cells w/
6 CA
o|
0|
o|
o|
o|
o|
o|
0|
o|
0|
1
0(
o|
0|
0|
0|
o|
o|
o|
o|
0|
1
0|
o|
0|
o|
0|
o|
0|
o|
0|
o|
1
0|
0|
0 1
Q j
Q 1
0|
o|
o|
Q I
Q 1
1
Q 1
Q I
31
0|
01
"I
0|
o|
0|
"I
1
1
cells w/
7 CA
o|
o|
o|
o|
o|
o|
o|
o|
0|
0|
1
0|
o|
o|
0|
0|
o|
Q 1
Q 1
0|
o|
1
0|
o|
o|
0|
"I
o|
o|
0|
o|
o|
1
0|
0|
o|
o|
o|
°l
Q 1
Q 1
Q 1
Q I
1
"I
0|
°l
0|
o|
01
0|
o|
o|
0|
1
1
cells w/
8 CA
0|
o|
o|
°l
o|
°l
°l
0|
0|
0|
1
0|
o|
0|
o|
o|
o|
°l
o|
0|
o|
1
°l
°l
o|
0|
°l
0|
0|
o|
0|
o|
1
°l
o|
"1
of
0|
o|
0 |
Q 1
o|
0|
1
0|
01
0|
o|
o|
o|
o|
0|
0|
0|
1
1
cells w/
9 CA
o|
o|
o|
Q |
Q 1
°l
o|
°l
0|
o|
1
0 |
Q I
o|
01
"I
o|
°l
0|
0|
o|
1
0|
°l
0|
o|
0|
o|
o|
0|
"I
0|
1
01
Q |
Q 1
o|
0|
o|
o|
"I
o|
0|
1
o|
o|
3|
°l
o|
0|
0|
0|
0|
0|
1
1
cells u/
10+ CA
o|
o|
0|
0|
0|
o|
o|
0|
0|
0|
I
°l
o|
0 I
Q 1
0|
o|
°l
o|
0|
o|
I
o|
o|
0|
°l
o|
0|
°l
°l
o|
0|
I
o|
°l
0|
o|
o|
0|
°l
o|
0|
Jl
I
°l
f|
°l
0|
o|
°l
o|
o|
o|
0|
I
I
pet. CA
positive
0.0|
o.oj
4.0|
0.0|
0.0|
12. OJ
16. OJ
24. OJ
o.oj
8.0 j
I
16. 0|
20. OJ
32. OJ
12. OJ
12. OJ
36. OJ
8.0|
28. OJ
16. OJ
12. OJ
1
0.0|
o.oj
o.oj
4.0|
0.0|
4.0|
4.0|
8.0|
o.oj
8.0J
1
8.0|
12. OJ
24. OJ
o.oj
8.0J
4.0|
12. 0|
28. OJ
16. 0|
20. OJ
1
0.0|
0.0|
4.0|
4.0|
4.0|
0.0|
0.0|
4.0|
8.0|
4.0|
1
1
HI
# cells
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
1
500)
500 j
500 1
500 j
500 j
500 j
500 j
sooj
500 j
500 j
1
500 1
500J
sooj
sooj
sooj
sooj
sooj
sooj
sooj
sooj
1
500 1
sooj
sooj
sooj
500 1
sooj
sooj
sooj
sooj
sooj
1
500)
500 1
500 1
500 1
sooj
500 1
500 1
500 1
500 1
500 1
1
1
HI
K meta
25 1
35 1
32 1
24 1
26 1
15|
18|
17|
16|
14|
I
12|
10)
15|
18|
10|
9|
5|
2|
1|
8|
I
25 1
26 1
24 1
25 1
28 1
32 1
15|
24 1
25 1
15|
I
25 1
15|
1*1
15)
12|
11|
12I
13 1
10|
2|
I
25 1
32 1
25 1
23|
21 1
2M
25 1
25 1
25 1
21I
1
1
HI
percent
S.00|
7.00J
6.40J
4.80J
S.20J
3.00J
3.60J
3.40J
3.20J
2. 80 j
1
2.40[
2.00J
3.00J
3.60J
2.00J
1.80J
i.ooj
0.40J
0.20J
1.60|
1
s.ooj
S.20J
4.80J
s.ooj
S.60J
6.40J
3.00J
4.80|
s.ooj
3.00J
1
5.00|
3. 00 j
2. 80 j
3. 00 j
2.40J
2.20J
2.40J
2.60J
2.00J
0.40J
1
S.OOj
6.40J
S.OOj
4.60|
4.20J
4.80|
S.00|
S.OOj
s.ooj
4.20|
1
1
15

-------
Animal    Slide
                    Scorer    Treat.
                              Code
                                        Sex
                                                  Dose
Sample    Nunber    chr-tid   ehr-some  chr-tid
                                                            Time
                                                                     Cells
                                                                                                   break
201
202
203
204
205
206
207
208
209
210

211
212
213
2U
215
216
217
218
219
220

221
222
223
224
225
226
227
228
229
230

231
232
233
234
235
236
237
238
239
240

241
242
243
244
245
246
247
248
249
250
1124|
1125J
1126J
1127J
1128 j
1129J
1130J
1131 1
1132 1
1133 1
1
1134|
1135 1
1136 1
1137J
1133 1
1139J
1140J
1141J
1142|
1143|
1
1144|
1145|
1146|
1H7J
1148 1
1149 1
1150)
1151|
1152|
1153|
1
1154|
1155|
1156 j
1157J
1158 1
1159|
1160 1
1161 1
1162 1
1163 1
I
1164 1
1165 1
1166 1
1167)
1168 1
1169)
1170)
1171 1
1172)
1173|
1
B
B|
B|
B
•1
•1
B|
B
B
B

B
•1
B
•1
•1
B
•1
B
B
B

B
B
B|
•1
•1
•1
•1
•1
B
B|

B|
B
B
B
B
B
•1
•1
•1
B|

•1
s|
B
B|
B
B|
B|
•1
•1
•1
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
BN|
BN|
1
BN|
BN|
8N|
BN|
BNJ
BN|
BN|
BN|
8N|
BN|
1
BM|
BM|
BH|
BN|
BN|
BN|
BN|
BM|
BN|
BN|
1
BN|
BN|
BN|
BN|
BN|
BN|
BN|
BN|
BN|
BN|
1
BN|
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
BN|
1
1
t
t
t
t
t
t
t
t
t
t

c
c
c
c
c
t
t
t
t
t

t
t
t
t
t
t
t
t
t
t

c
c
c
c
c
t
t
t
t
t

t
t
*
t
t
t
t
t
t
t
1 '1
1 '1
*l
1 fl
1 fl
1 '1
1 
-------

201
202
203
204
205
206
207
206
209
210

211
212
213
214
215
216
217
218
219
220

221
222
223
224
225
226
227
228
229
230

231
232
233
234
235
236
237
238
239
240

241
242
243
244
245
246
247
248
249
250
chr-soM
break
0|
"I
0|
o|
o|
11
o|
°l
»l
o|
I
0|
o|
0|
°l
«l
°l
o|
o|
o|
°l
I
II
o|
0|
0|
11
11
0|
0|
o|
11
I
o|
°l
°l
o|
0|
°l
0|
Jl
o|
o|
I
11
0|
0|
°l
0|
11
0|
°l
o|
0|
I
I
chr-tid
exchange
o|
0|
°l
0|
°l
»l
"I
0|
0|
11
I
°l
0|
0|
o|
«l
°l
0|
o|
o|
o|
I
o|
"I
o|
0|
11
0|
0|
o|
o|
0|
I
0|
o|
0|
0|
°l
«l
o|
0|
o|
o|
I
0|
0|
0|
0|
o|
o|
o|
0|
"I
2|
I
I
chr-some
exchange
0|
0|
0|
0|
0|
0|
0|
o|
°l
«l
I
0|
°l
°l
0|
"I
0|
° I
°l
o|
0|
I
o|
o|
0|
0|
0|
°l
o|
0|
o|
»l
I
o|
0|
o|
o|
°l
«l
o|
Jl
o|
o|
I
0|
»l
0|
°l
"I
0|
°l
°l
0|
0|
I
I
other
aberratn
0|
o|
0|
0|
o|
0|
»l
0|
0|
0|
I
0|
o|
°l
0|
o|
0|
o|
0|
o|
°l
I
0|
0|
»l
0|
o|
0|
0|
o|
0|
o|
I
o|
o|
°l
0|
0|
o|
°l
o|
o|
°l
I
0|
°l
°l
o|
o|
0|
0|
°l
o|
°l
I
I
CA/cell
exc gap
0.040)
0.040 j
0.080 |
0.120 |
0.040J
0.080)
0.120)
0.160 |
0.120J
0.080 j
I
0|
0|
"I
0|
o|
0.080J
0.040 j
°l
0.080)
0.040 |
I
o.osoj
0.040)
0.080)
0.120 |
0.120)
0.240)
0.160 |
0.200)
0|
0.240)
1
0)
o|
0|
0.080)
0|
0.040)
0.040)
0.040)
0.080)
°l
1
0.120)
0.120)
0.120)
0.040 |
o|
0.240)
0.120)
0.040)
0.160)
0.200|
I
I
celts w/
0 CA
24)
• 24)
23 1
22 1
24)
23)
22)
21 1
22)
23 1
1
25)
25)
25)
25)
25 1
23)
24)
25 1
23)
24 1
1
23|
24)
23)
22 1
22 1
19|
21 1
20)
25)
19)
I
25)
25 1
25 1
23)
25)
24)
24)
24|
23 1
25)
I
22)
22 1
22)
24)
25)
19)
22 1
24 1
21 1
20)
I
I
celts w/
1 CA
1|
1|
2)
3|
1|
2|
3|
«l
3|
2|
I
o|
°l
o|
0|
"I
2|
1|
°l
2|
1|
1
2)
M
2|
3|
3|
*l
«l
5)
°l
6|
I
0|
o|
0|
2|
0|
1|
1|
M
2)
0|
I
3)
3)
3)
1|
o|
6|
3)
1|
*l
5)
I
I
cells w/
2 CA
0|
0|
"I
o|
0|
o|
0|
0|
o|
o|
I
o|
0|
o|
0|
o|
0|
0|
"I
o|
o|
I
0|
o|
°l
0|
0)
0|
«l
o|
°l
0|
I
0|
0|
0|
o|
0|
o|
0|
0|
0|
0|
I
o|
0|
0|
0|
0|
°l
0|
0)
o|
o|
I
I
cells y/
3 CA
o|
0|
0|
0|
0|
0|
0)
o|
o|
0|
I
o|
0|
0|
0|
»l
0|
"I
0|
0|
0|
I
o|
o|
o|
0|
"I
0|
»l
"I
o|
»l
I
o|
"I
o|
0|
.o|
0|
o|
o|
0|
o|
I
°l
°l
0|
0|
°l
0)
0|
°l
o|
0|
I
I
cells w/
4 CA
°l
°l
°l
«l
0|
0|
0|
o|
0|
o|
I
o|
o|
o|
o|
0|
«l
o|
o|
0|
o|
I
0)
o|
o|
0|
0|
0|
"I
o|
0|
o|

o|
0|
o|
0|
0|
o|
0|
»l
"I
0|

0|
0|
o|
3|
0|
0|
0|
°l
0|
0|
cells u/
5 CA
o|
o|
o|
0|
o|
0|
o|
0|
o|
«l
I
"I
o|
0|
0|
0|
«l
0|
°l
0)
o|
I
o|
0|
0|
0|
0|
o|
0|
o|
0|
o|
I
0)
°l
0|
0|
0|
0|
0|
o|
"I
o|
I
0|
o|
o|
o|
o|
»l
o|
0|
0|
»l
I
I
17

-------

201
202
203
204
205
206
207
208
209
210

211
212
213
2U
215
216
217
218
219
220

221
222
223
224
225
226
227
228
229
230

231
232
233
234
235
236
237
238
239
240

241
242
243
244
245
246
247
248
249
250

cells w/
6 CA
°l
0|
°l
°l
0|
"I
°l
o|
0|
o|
I
"I
0|
o|
°l
0|
°l
0|
0|
°l
o|
I
0|
0|
0|
0|
°l
o|
°l
°l
o|
0|
I
o|
°l
o|
o|
o|
o|
o|
°l
°l
o|
I
o|
o|
°l
o|
°\
o|
o|
°l
o|
o|
I
I
cells w/
7 CA
0|
o|
o|
0|
0|
o|
0|
°l
o|
o|
I
o|
o|
0|
o|
0|
o|
0|
0|
o|
°l
I
°l
°l
0|
°l
°l
o|
o|
°l
0|
o|
I
o|
o|
°l
o|
o|
o|
o|
o|
o|
°l
I
o|
"I
°l
°l
°l
°l
o|
o|
o|
°l
I
I
cells w/
8 CA
°l
o|
o|
0|
o|
°l
o|
o|
"I
0|
I
°l
o|
0|
0|
0|
°l
o|
0|
o|
"I
I
0|
°l
o|
°l
°l
0|
°l
o|
0|
o|
I
°l
°l
0|
0|
o|
°l
«l
0|
°l
o|
I
"I
o|
0|
o|
o|
°l
°l

-------
Animal Slide Scorer Treat. Sex Dose Sample Number chr-tid chr-some chr-tid
Code Time Cells gap gap break
251
252
253
254
255
256
257
258
259
260


1174|
1175J
1176J
1177)
1178 1
1179J
1180 j
1181|
1182 j
1183 1
1
1
B|
B|
B|
B|
Bl
B|
B|
B|
B|
B|
1
1
BK|
BX|
BN|
BN|
BN|
BN|
BN|
BNJ
BN|
BN|
1
1
P|
P|
Pi
Pi
Pi
P|
P|
Pi
Pi
Pi
1
1
m| 12. 5|
mj 12. 5|
mj 12.5J
mj 12. 5|
m| 12. 5|
f| 12.5|
f| 12. 5|
fj 12. 5|
fj 12. 5|
fj 12. 5|
I I
I I
48 1
48 1
48 1
48|
48|
48 1
48|
48|
48|
48 1
I
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
I..
o|
3|
0|
1|
0|
o|
5|
o|
0|
4|
I
I
"I
o|
0|
o|
o|
0 1
Q 1
Q 1
o|
0|
I
I
'I
7|
5|
0|
4|
4 1
3|
n
4|
5|
I
I
19

-------
chr-some ehr-tid chr-some other CA/cell cells w/ cells u/ cells w/ cells w/ cells w/ cells w/
break exchange exchange aberratn exc gap 0 CA 1 CA 2 CA 3 CA 4 CA 5 CA
251 |
252 |
253 |
254 |
255 |
256 |
257 |
258 |
259 j
260 j
I
I
2|
3|
1|
o|
o|
1|
o|
°l
o|
o|
1
I
3|
1|
o|
0|
»l
o|
°l
o|
o|
°l
I
I
»l
0|
o|
0|
°l
0|
"I
o|
o|
0|
I
I
o|
o|
0|
0|
0|
0|
o|
"I
0|
°l
I
I
0.560|
0.440|
0.240 j
o|
0.160J
0.200J
0.120J
0.280 j
0.160 |
0.200 |
I
I
14|
15|
19|
25 1
21 1
20 1
22 1
18|
21 1
20 1
1
1
8|
'1
«l
0|
*l
5|
3|
n
*l
5|
1
1
3|
M
°l
0|
0|
0|
o|
o|
«l
o|
I
I
o|
0|
0|
»l
o|
°l
0|
"I
o|
o|
I
I
°l
0|
0|
o|
0|
«l
0|
o|
o|
o|
I
I
o|
0|
0|
o|
0|
o|
0|
"I
«l
o|
I
I
20

-------
cells u/ cells M/ cells u/ cells w/ cells w/ pet. CA
6 CA 7 CA 8 CA 9 CA 10+ CA positive #
25 1
252
253
254
255
256
257
258
259
260


01
0|
0|
o|
o|
0|
o|
o|
o|
o|
1
1
0|
0|
«l
°l
0|
«l
0|
o|
o|
0|
1
1
0|
0|
o|
o|
0|
»l
"1
0|
0|
0|
1
1
0|
0|
0|
0|
o|
0|
o|
0|
o|
0|
1
1
0|
0|
0|
0|
0|
o|
°l
o|
"1
0|
1
1
44.0)
40. OJ
24.0 1
0.0|
16. OJ
20. 0|
12. OJ
28.0 j
16. 0|
20. 0|
1
1
MI MI HI
cells # meta percent
500)
500 j
500 [
500 j
500)
500 1
500 1
500 j
500 j
500 j
1
L
ts|
25 1
32 1
32 1
25 1
24 1
26 1
28|
29 1
27|
1
1
3.00|
5.00|
6.40J
6.40|
5. 00 1
4.80|
5. 20 j
5. 60 j
5. 80 j
5.40J
1
1
21

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex       Pet.     Mean Pet.
   1      3     0.0  24.0    m     60.000        13.600
   2    133     0.0  24.0    m     80.000        17.600
                                      22

-------
filename:  C:\CA\CATEST01.ILS   date:              time:
    Endpoint:  Chromosomal aberration (pet. damaged cells)

  Variance inflation factor:    1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           30.014    24   0.1843
  Sex              8.226    12   0.7673
  Time            30.904     8   0.0001
                                       23

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time =    24.00 (Males)  for 067889
   P
   alpha
        0.000
        0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
4
8
15
31
Cells
Scored
200
250
250
250
Percent
Aberrant
2.0000
3.2000
6.0000
12.4000
SEM
(for Obs)
1.0690
0.9978
1.4907
1.9276
                                                           Pairwise
                                                       Significance

                                                             0.2162
                                                             0.0180
                                                             0.0000
Time
   P
   alpha
48.00 (Males)  for 067889

        0.000
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Aberrant
    Cells
        3
       20
       36
       47
 Cells
Scored
   250
   250
   250
   250
 Percent
Aberrant
  1.2000
  8.0000
 14.4000
 18.8000
      SEM
(for Obs)
   0.6110
   2.3851
   2.6800
   2.9242
    Pairwise
Significance

      0.0001
      0.0000
      0.0000
                                       24

-------
filename: C:\CA\CATEST01.ILS   date:
                                time:
Time =    72.00 (Males)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
2
9
24
39
Cells
Scored
250
250
. 250
250
Percent
Aberrant
0.8000
3.6000
9.6000
15.6000
SEN
(for Obs)
0.5333
0.9333
2.3247
2.8875
Pairwise
Significance

0.0164
0.0000
0.0000



*
*
*
Time =    24.00 (Females)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged  cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
3
7
13
25
Cells
Scored
250
250
250
250
Percent
Aberrant
1.2000
2.8000
5.2000
10.0000
SEM
(for Obs)
0.6110
0.8537
1.0414
1.2293
Pairwise
Significance

0.1007
0.0055
0.0000




*
*
                                        25

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time
   P
   alpha
48.00 (Females)  for 067889

        0.000
   =    0.040  (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet.  damaged cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
3
17
25
34
Cells
Scored
250
250
250
250
Percent
Aberrant
1.2000
6.8000
10.0000
13.6000
SEM
(for Obs)
0.8537
1.2000
1.4907
3.0521
Pairwise
Significance

0.0007
0.0000
0.0000
Time =    72.00 (Females)  for 06788S

   p         =    0.000
   alpha     =    0.040  (scaled by .8, assuming next run to exclude high  dose)

   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
4
11
19
35
Cells
Scored
250
250
250
250
Percent
Aberrant
1.6000
4.4000
7.6000
14.0000
SEM
(for Obs)
0.8844
0.9333
1.3920
2.2509
Pairwise
Significance

0.0332
0.0007
0.0000
                                       25

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)
  There are 2 outliers at the .05 significance level.

       Line    Dose  Time  Sex       Pet.     Mean Pet.
   1      3     0.0  24.0    m     60.000        13.600
   2    133     0.0  24.0    m     80.000        17.600
                                       27

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)

  Variance inflation factor:     1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           30.014    24   0.1843
  Sex              8.226    12   0.7673
  Time             30.904     8   0.0001
                                       28

-------
filename: C:\CA\CATEST01.ILS   date:
                                time:
Time =    24.00 (Both sexes)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged  cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
7
15
28
56
Cells
Scored
450
500
500
500
Percent
Aberrant
1.5556
3.0000
5.6000
11.2000
SEM
(for Obs)
0.5729
0.6407
0.8897
1.1462
Pairwise
Significance

0.0697
0.0005
0.0000




*
*
Time =    48.00 (Both sexes)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged  cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
6
37
61
81
Cells
Scored
500
500
500
500
Percent
SEM
Pairwise
Aberrant (for Obs) Significance
1.2000
7.4000
12.2000
16.2000
0.5109
1.3067
1.5755
2.1418

0.0000
0.0000
0.0000
                                       29

-------
filename:  C:\CA\CATEST01.ILS   date:              time:


Time =    72.00 (Both sexes)  for 067889

   p         =    0.000
   alpha     =    0.040 (scaled by .8, assuming next run to exclude high dose)

   One tailed test (binomial)  for chromosomal aberration (pet. damaged cells)
           Aberrant      Cells    Percent        SEM       Pairwise
   MG/KG      Cells     Scored   Aberrant  (for Obs)   Significance
    0.00          6        500     1.2000     0.5109
  500.00         20        500     4.0000     0.6489         0.0027   *
 1000.00         43        500     8.6000     1.3385         0.0000   *
 2000.00         74        500    14.8000     1.7912         0.0000   *
                                       30

-------
filename:  C:\CA\CATEST01.ILS   date:              time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex       Pet.     Mean Pet.
   1      3     0.0  24.0    m     60.000        13.600
   2    133     0.0  24.0    m     80.000        17.600
                                       31

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time =

   alpha
48.00 (Males)

        0.050
for 067889
   One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
     (with positive controls)
           Aberrant      Cells    Percent        SEM
   MG/KG      Cells     Scored   Aberrant  (for Obs)
    0.00          3        250     1.2000     0.6110
   12.50         67        250    26.8000     4.3019
                                                 Pairwise
                                             Significance

                                                   0.0000
Time =    48.00 (Females)  for 067889

   alpha     =    0.050

   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)
     (with positive controls)
           Aberrant      Cells    Percent        SEM
   MG/KG      Cells     Scored   Aberrant  (for Obs)
    0.00          3        250     1.2000     0.8537
   12.50         56        250    22.4000     2.2470
                                                 Pairwise
                                             Significance

                                                   0.0000
                                       32

-------
filename:  C:\CA\CATEST01.ILS   date:
                                  time:
    Endpoint:  Chromosomal aberration (total aberrations)
  Variance inflation factor:
  Alpha:
  Factor

  Scorer
  Sex
  Time
Deviance

  27.057
   4.281
  21.975
df

24
12
 8
                1.000
                0.050
0.3018
0.9778
0.0050
                                        33

-------
filename: C:\CA\CATEST01.ILS   date:
                                time:
Time -    24.00 (Males)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          4
  500.00          8
 1000.00         15
 2000.00         32
       Cells Aberrat'ns
SEM
Pairwise
Scored
200
250
250
250
/ Cell
0.0200
0.0320
0.0600
0.1280
(for Obs)
0.0107
0.010
0.0149
0.0222
Significance

0.2193
0.0201
0.0000



*
*
Time =    48.00 (Males)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          3
  500.00         20
 1000.00         39
 2000.00         51
       Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell (for Obs) Significance
0.0120
0.0800
0.1560
0.2040
0.0061
0.0239
0.0340
0.0318

0.0002
0.0000
0.0000
                                        34

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time
   P
   alpha
72.00 (Males)  for 067889

        0.000
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          2
  500.00          9
 1000.00         25
 2000.00         44
               Cells Aberrat'ns
              Scored
                 250
                 250
                 250
                 250
/ Cell
0.0080
0.0360
0.1000
0.1760
      SEM
(for Obs)
   0.0053
   0.0093
   0.0262
   0.0359
    Pairwise
Significance

      0.0174
      0.0000
      0.0000
Time =    24.00 (Females)  for 067889
   P
   alpha
        0.000
        0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          3
  500.00          7
 1000.00         13
 2000.00         25
               Cells Aberrat'ns
              SEM
Scored
250
250
250
250
/ Cell
0.0120
0.0280
0.0520
0.1000
(for Obs)
0.0061
0.0085
0.0104
0.0123
                Pairwise
            Significance

                  0.1030
                  0.0062
                  0.0000
                                         35

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time =    48.00 (Females)  for 067889
   P
   alpha
        0.000
        0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          3
  500.00         17
 1000.00         25
 2000.00      .   34
               Cells Aberrat'ns
Scored
   250
   250
   250
   250
/ Cell
0.0120
0.0680
0.1000
0.1360
      SEM
(for Obs)
   0.0085
   0.0120
   0.0149
   0.0305
          Pairwise
      Significance

            0.0009
            0.0000
            0.0000
                                                            *
                                                            *
Time
   P
   alpha
72.00 (Females)  for 067889

        0.000
   =    0.040  (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          4
  500.00         11
 1000.00         19
 2000.00         35
               Cells Aberrat'ns
SEM
Scored
250
250
250
250
/ Cell (for Obs)
0.0160
0.0440
0.0760
0.1400
0.0088
0.0093
0.0139
0.0225
                                   Pairwise
                               Significance

                                     0.0354
                                     0.0009
                                     0.0000
                                        36

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)
  There are 2 outliers at the .05 significance level.

       Line    Dose  Time  Sex   Abs/Cell  Av Abs/Cell
   1      3     0.0  24.0    m      0.600        0.136
   2    133     0.0  24.0    m      0.800        0.176
                                        37

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)

  Variance inflation factor:    1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           27.057    24   0.3018
  Sex              4.281    12   0.9778
  Time            21.975     8   0.0050
                                        38

-------
filename: C:\CA\CATEST01.ILS   date:
                                time:
Time =    24.00 (Both sexes)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          7
  500.00         15
 1000.00         28
 2000.00         57
       Cells Aberrat'ns
SEM
Pairwise
Scored
450
500
500
500
/ Cell (for Obs) Significance
0.0156
0.0300
0.0560
0.1140
0.0057
0.0064
0.0089
0.0127

0.0720
0.0006
0.0000
Time =    48.00 (Both sexes)  for 067889
   P
   alpha
0.000
0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          6
  500.00         37
 1000.00         64
 2000.00         85
       Cells Aberrat'ns
SEM
Pairwise
Scored
500
500
500
500
/ Cell
0.0120
0.0740
0.1280
0.1700
(for Obs)
0.0051
0.0131
0.0192
0.0228
Significance

0.0000
0.0000
0.0000
                                        39

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex   Abs/Cell  Av Abs/Cell
   1      3     0.0  24.0    m      0.600        0.136
   2    133     0.0  24.0    m      0.800        0.176
                                        40

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time =

   alpha
48.00 (Males)

        0.050
for 067889
   One tailed test (Poisson) for chromosomal aberration (total aberrations)
     (with positive controls)
              Total
   MG/KG Aberrat'ns
    0.00          3
   12.50         74
               Cells Aberrat'ns
              Scored     / Cell
                 250     0.0120
                 250     0.2960
                        SEM
                  (for Obs)
                     0.0061
                     0.0531
    Pairwise
Significance

      0.0000
Time =

   alpha
48.00 (Females)

        0.050
  for 067889
   One tailed test (Poisson) for chromosomal aberration (total aberrations)
     (with positive controls)
              Total
   MG/KG Aberrat'ns
    0.00          3
   12.50         58
               Cells Aberrat'ns        SEM
              Scored     / Cell  (for Obs)
                 250     0.0120     0.0085
                 250     0.2320     0.0259
                                  Pairwise
                              Significance

                                    0.0000
                                       41

-------
filename: C:\CA\CATEST01.ILS   date:              time:


Time =    72.00 (Both sexes)  for 067889

   p         =    0.000
   alpha     =    0.040 (scaled by .8, assuming next run to exclude high dose)

   One tailed test (Poisson) for chromosomal aberration (total aberrations)
              Total      Cells Aberrat'ns        SEM       Pairwise
   MG/KG Aberrat'ns     Scored     / Cell  (for Obs)   Significance
    0.00          6        500     0.0120     0.0051
  500.00         20        500     0.0400     0.0065         0.0030   *
 1000.00         44        500     0.0880     0.0147         0.0000   *
 2000.00         79        500     0.1580     0.0210         0.0000   *
                                       42

-------
filename:  C:\CA\CATEST01.ILS   date:               time:
    Endpoint:  Mitotic Index

  Variance inflation factor:     1.341
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           52.058    24   0.0008
  Sex            206.145    24   0.0000
  Time           136.872    32   0.0000
                                      43

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time
24.00 (Males)  for 067889
P
alpha
0.768
0.050
Likelihood ratio for

MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
196
218
213
202


mitotic
Cells
Scored
5000
5000
5000
5000


index
Mitotic
Index (%)
3.9200
4.3600
4.2600
4.0400



SEM
(for Obs)
0.5531
0.3874
0.2876
0.3745
                                                           Pairwise
                                                       Significance

                                                             0.8298
                                                             0.7707
                                                             0.6045
Time
   P
   alpha
48.00 (Males)  for 067889

        0.000
        0.050
   Likelihood ratio for mitotic index

MG/KG
0.00
500.00
1000.00
2000.00
Cells in
Mitosis
264
179
140
64
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.2800
3.5800
2.8000
1.2800
SEM
(for Obs)
0.3492
0.1849
0.1789
0.1937
                                                           Pairwise
                                                       Significance

                                                             0.0002
                                                             0.0000
                                                             0.0000

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time
   P
   alpha
72.00 (Males)  for 067889

        0.000
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      230
      206
      152
       76
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.6000
4.1200
3.0400
1.5200
SEM
(for Obs)
0.3876
0.3441
0.2596
0.2585
                                   Pairwise
                               Significance

                                     0.1551
                                     0.0002
                                     0.0000
Tine =    24.00 (Females)  for 067889
   P
   alpha
        0.697
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      256
      241
      236
      227
 Cells
Scored
  5000
  5000
  5000
  5000
  Mitotic
Index (%)
   5.1200
   4.8200
   4.7200
   4.5400
      SEM
(for Obs)
   0.2313
   0.1093
   0.1794
   0.3724
    Pairwise
Significance

      0.2756
      0.2123
      0.1214
                                       45

-------
filename: C:\CA\CATEST01.ILS   date:
                                        time:
Time
   P
   alpha
48.00 (Females)  for 067889

        0.572
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      253
      239
      232
      219
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.0600
4.7800
4.6400
4.3800
SEM
(for Obs)
0.4927
0.3299
0.5504
0.4263
    Pairwise
Significance

      0.2881
      0.1993
      0.0831
Time =    72.00 (Females)  for 067889
   P
   alpha
        0.707
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      222
      212
      240
      224
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.4400
4.2400
4.8000
4.4800
SEM
(for Obs)
0.4020
0.3180
0.1978
0.3593
    Pairwise
Significance

      0.3359
      0.7705
      0.5333
                                       46

-------
filename: C:\CA\CATEST01.ILS   date:
                                       time:
Time =    48.00 (Males)  for 067889

   alpha     =    0.050

   Likelihood ratio for mitotic index   (with positive controls)
                                                           Pair-wise
                                                       Significance

                                                             0.1692

MG/KG
0.00
12.50
Cells in
Mitosis
264
243
Cells
Scored
5000
5000
Mitotic
Index (%)
5.2800
4.8600
SEM
(for Obs)
0.3492
0.4483
Time =    48.00 (Females)  for 067889

   alpha     =    0.050

   Likelihood ratio for mitotic index
                              (with positive controls)
   MG/KG
    0.00
   12.50
Cells in
 Mitosis
     253
     253
 Cells
Scored
  5000
  5000
  Mitotic
Index (%)
   5.0600
   5.0600
      SEM
(for Obs)
   0.4927
   0.1492
    Pairwise
Significance

      0.5000

-------
SAMPLE TEST DATA




    CATEST02

-------
C:\CA\CATEST02.ILS listed at
                on
EXPERIMENT 	
Record 	
Laboratory 	
Lab Book 	
Date Started ...
Date Completed  .
Slides Coded By
Staining Method
Scored By 	
Entered By 	
Entry Date 	
Proofed By 	
Remarks
CA-TEST 02
121
ILS
III
06/18/90
08/01/90
MP
GIEMSA
CJH
MP
07/25/90
DC
TEST ARTICLE 	
Receipt Date ......
CAS # 	
Source / Lot 	
Appearance 	
Purity 	
Stability 	
Storage Conditions
Solubility 	
Hazard Information

Remarks 	
067889
04/20/90
UNKNOWN
RADIAN 067H3
ORANGE POWDER
UNKNOWN  '
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER
TREAT AS CARCINOGEN
                                   48

-------
VEHICLE 	,
Source / Lot	,
Purity 	,
Stability	,
Storage Conditions
CORN OIL
SIGMA/13F100
UNKNOWN
1 YEAR
ROOM TEMPERATURE
Remarks
POSITIVE CONTROL . .
Receipt Date 	
CAS # 	
Source / Lot 	
Appearance 	
Purity 	
Stability 	
Storage Conditions
Solubility 	
Hazard Information

Remarks 	
991870
01/22/90
UNKNOWN
RADIAN/110BD
WHITE POWDER
UNKNOWN
1 YEAR
ROOM TEMPERATURE
INSOLUBLE IN WATER
CARCINOGEN
                                     49

-------
TEST SYSTEM
Species
Strain 	
Supplier ...
Received ...
Quarantined
 From
Until
MOUSE
B6C3F1
TACONIC FARMS
05/01/90
05/01
05/15
Routine Husbandry Conditions?  (y/n)   Y
                           Age
                    Sex  Fr.  To
                     M  11   11
                     F  11   11

              Age units WEEKS
                        Weight
                       Fr.  To
                      32.0 34.0
                      27.0 29.0

                      Weight units GRAM
Remarks
TREATMENT
Date Started 	
Date Completed ...
Route 	
Volume 	
Doses 	
Dose Units 	
Number Treatments
Treatment Duration
Treatment Date ...
Treatment Interval
Number Samples ...
Sample Date 	
Sample Interval ..
Tissue Cell Type

Remarks 	
        06/18/90
        06/21/90
        IP
        0.4
        0, 500, 1000, 2000
        MG/KG
        1
        NA
        06/18/90
        NA
        3
        06/19;06/20;06/21
        24 HR
                                     50

-------
Animal    Slide     Scorer    Treat.    Sex       Dose      Sample    Number    chr-tid   chr-some  chr-tid
                              Code	Time	Cells	gap	gap	break
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20

21
22
23
24
25
26
27
28
29
30

31
32
33
34
35
36
37
38
39
40

41
42
43
44
45
46
47
48
49
50


1054 1
1055 j
1056 1
1057J
1058)
1059)
1060J
1061 1
1062 1
1063 j
i
I
1064 1
1065 1
1066 j
1067 j
1068 j
1069 1
1070 j
1071 j
1072|
1073 j
I
1074 1
1075 1
1076 j
1077J
1078 1
1079J
1080 1
1081 j
1082 1
1083 1
I
1084|
1085|
1086|
1087|
1088)
1089 j
1090J
1091 1
1092 1
1093|
1
1094|
1095 1
1096 1
1097|
1098 1
1099)
1100 1
1101|
1102)
1103 j
I
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
I
I
I
I
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A
A
A|

A|
A|
A|
A|
A
A
A|
A|
A|
A
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP)
HP |
HP|
HP |
HP|
HP |
HP |
HP |
HP |
I
HP |
HP |
HP |
HP |
HP j
HP |
HP |
HP |
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
MP|
HP |
IP)
HP|
1
1
c|
c|
e|
c|
c|
tl
tl
tl
tl
1
tl
tl
tl
tl
t
tj
tl
tl
tl
tl
1
c|
c|
c|
c|
c
tl
tl
tl
tl
tl
1
tl
tl
tl
tl
tl
t
tl
tl
tl
t

c
c
c
c
c
t
t
t
t
t


m|
m|
m|
m|
m|
m|
m|
m|
m|
m|
I
1
m|
m|
m|
m|
H
H
m|
m|
m|
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1
m|
m|
m|
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m|
m|
m|
m|
m|
m|
1
m|
m|
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m|
m|
m|
m|
m|
m|
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1
m|
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m|
m|
m|
H
m|
m|
m|
m|
1
1
0|
o|
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o|
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500)
500 1
500 j
500 j
500 j
1
1
1000|
1000 1
1000)
1000 j
1000 1
2000)
2000 j
2000 1
2000 j
2000 j
I
0|
o|
0|
Q 1
Q I
500 1
500 j
500 j
500 j
500 1
I
1000 1
1000 j
1000 j
1000 j
1000 1
2000 j
2000 1
2000 1
2000 1
2000 j
I
0|
o|
o|
o|
°l
500 j
500 1
500 1
500 1
500 1
I
I
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24|
24 1
24 1
24 1
24 1
24 1
24 1
24 1
I
48 1
48 1
48|
48 1
48 1
48 1
48|
48|
48|
48 1
I
48 1
48 1
48 1
48|
48|
48|
48 1
48 1
48|
48|
I
72|
72|
72|
72|
72)
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72!
72|
72!
72|
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25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25|
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
I
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
25 1
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l
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1|
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0 1
0 1
"I
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11
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11
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Q |
Q I
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Q |
Q I
o|
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0|
0|
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o|
oi
0|
0|
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Q 1
Q 1
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0|
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Q I
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Q |
Q I
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Q 1
Q I
o|
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o|
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I
2|
2|
15|
2|
2|
0|
°l
0|
11
1
1
2|
1|
0|
1|
1|
6I
3|
2|
1|
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1|
3|
2|
2|
M
1|
2|
o|
1|
1|
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1|
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2|
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3|
1|
11
M
°!
"1
M
M
I
I
                                                       51

-------

1
2
3
4
5
6
7
8
9
10

11
12
13
U
15
16
17
18
19
20

21
22
23
24
25
26
27
28
29
30

31
32
33
34
35
36
37
38
39
40

41
42
43
44
45
46
47
48
49
50
chr-some
break
0|
0|
o|
o|
o|
o|
o|
"I
o|
0|
I
0|
o|
°l
°l
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0|
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0|
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0|
0|
o|
0|
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o|
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o|
0|
o|
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0|
o|
0|
o|
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0|
I
°l
°l
°l
o|
o|
0|
o|
"I
o|
0|
I
I
chr-tid
exchange
o|
°l
o|
o|
o|
o|
o|
o|
o|
o|
I
°l
°l
°l
0|
°l
o|
o|
°l
o|
o|
I
o|
0|
o|
0|
0|
o|
0|
o|
o|
0|
I
o|
o|
o|
o|
o|
°l
°l
0|
o|
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I
o|
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a|
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0|
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chr-some
exchange
"I
o|
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0|
°l
0|
°l
o|
o|
°l
I
0|
o|
°l
°l
o|
o|
o|
o|
o|
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I
0|
o|
o|
°l
o|
°l
o|
o|
o|
°l
I
0|
0|
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0|
0|
°l
0|
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o|
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°l
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other
aberratn
°l
°!
°l
°l
°l
°l
"I
0|
o|
0|
I
"I
o|
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°l
"I
0|
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"I
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I
0|
°l
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0|
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0|
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0|
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o|
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o|
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0|
"I
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0|
0|
0|
I
°l
°l
o|
0|
°l
0|
«l
o|
0|
0|
I
I
CA/cell
exc gap
0.080 |
0.080 j
0.600 |
0.080 j
0.080 |
o|
o|
o|
0.040]
0.040 j
I
0.040 |
o.oaoj
0.040 j
»l
0.040)
0.040 |
0.160 j
0.120 j
0.080 j
0.040 j
I
0.040J
0.120 j
0.080 j
0.080J
0.040 |
0.040 |
0.080 |
o|
0.040|
0.040 |
I
0.040 |
0.080)
0.120 |
0.200J
o|
0.240|
0.120|
0.080 |
0.280 j
0.200 |
I
o.oeoj
o.oaoj
0.120|
0.040J
0.040|
0.040|
0|
0|
0.040|
0.040J
1
1
cells w/
0 CA
23 1
231
10|
23 1
23 1
25 1
25 1
25 1
24 1
24 1
I
24 1
24 1
24 1
25 1
24 1
24 1
24 1
22|
24 1
24 1
I
24 1
24 1
23 1
23 1
24 1
24 1
24 1
25 1
24 1
24 1
I
24 1
24 1
24 1
24 1
25 1
24 1
24 1
24 1
23 1
24 1
I
23 1
24 1
24 1
24 1
24 1
2* I
25 1
25 1
24 1
24 1
I
I
cells w/
1 CA
2|
2|
15|
2|
2|
o|
°l
0|
1|
1|
I
1|
o|
1|
0|
1|
1|
o|
0|
"1
'I
1
1|
o|
2|
2|
1|
1|
0|
o|
1|
1|
I
M
°l
°l
o|
0|
"I
f|
°l
o|
o|
I
2|
1|
o|
1|
1!
1|
o|
°l
1|
1|
I
I
cells w/
2 CA
°l
"I
"I
0|
°l
°l
°l
0|
o|
o|
I
0|
1|
o|
0|
o|
o|
0|
o|
1|
°l
1
0|
o|
o|
°l
o|
o|
M
o|
o|
o|
I
"I
'I
°l
o|
o|
o|
o|
II
11
"1
1
"1
°l
o|
o|
of
o|
°l
01
0|
o|
1
1
cells u/
3 CA
°l
°l
o|
o|
"I
o|
"1
o|
o|
01
1
°l
0|
0|
"I
o|
o|
0|
3|
0|
o|
1
o|
1|
0|
°l
0|
o|
0|
o|
o|
"I
I
o|
°l
11
0|
o|
o|
11
0|
o|
0|
I
0|
"I
II
0|
0|
0|
"1
°l
0|
0|
1
I
cells u/
4 CA
°l
0|
0|
o|
°l
0|
o|
o|
o|
o|
1
o|
"1
o|
0|
°l
0|
11
0|
0|

-------

1
2
3
4
5
6
7
8
9
10

11
12
13
U
15
16
17
18
19
20

21
22
23
24
25
26
27
28
29
30

31
32
33
34
35
36
37
38
39
40

41
42
43
44
45
46
47
48
49
50

cells w/
6 CA
0|
o|
0|
°l
o|
°l
o|
o|
°l
°l
1
o|
°l
o|
0|
o|
0|
o|
o|
0|
o|
1
0|
°l
0|
o|
o|
°l
°l
0|
0|
0|
1
°l
0|
0|
o|
0|
11
°l
°l
°l
o|
I
0|
o|
°l
0|
°l
0|
°l
o|
°l
°l
I
I
cells w/
7 CA
"I
o|
o|
°l
o|
o|
°l
°l
o|
o|
I
°l
°l
°l
°l
o|
o|
o|
°l
o|
°l
I
°l
o|
°l
o|
0|
°l
0|
o|
o|
°l
I
°l
°l
°l
°l
o|
o|
o|
°l
o|
o|
I
0|
o|
o|
°l
o|
°l
o|
"I
°l
°l
I
I
cells u/
8 CA
°l
0|
o|
°l
°l
"I
°l
0|
o|
°l
I
0|
0|
o|
"I
o|
°l
°l
o|
o|
°l
I
0|
0|
o|
o|
°l
°l
o|
°l
o|
"I
I
o|
o|
o|
o|
o|
"I
o|
o|
°l
o|
I
0|
o|
0|
°l
o|
0|
o|
o|
o|
o|

cells w/
9 CA
o|
o|
0|
"I
o|
°l
0|
o|
o|
"I
I
0|
0|
o|
o|
o|
o|
o|
°l
o|
"I
I
"I
0|
0|
o|
o|
o|
°l
o|
o|
o|
I
o|
o|
o|
o|
"I
o|
o|
o|
o|
o|
I
°l
o|
0|
o|
°l
^l
°l
°l
o|
°l
I
I
cells u/
10+ CA
o|
°l
o|
o|
°l
o|
0|
0|
°l
o|
I
o|
o|
"I
o|
o|
0|
°l
0|
o|
"I
I
o|
0|
o|
o|
o|
0|
o|
o|
o|
o|
I
o|
o|
o|
o|
o|
°l
o|
0|
o|
o|
I
o|
0|
o^
'l

-------

51
52
53
54
55
56
57
58
59
60

61
62
63
64
65
66
67
68
69
70

71
72
73
74
75
76
77
78
79
80

81
82
83
84
85
86
87
88
89
90

91
92
93
94
95
96
97
98
99
00


Animal Slide
1104 1
1105 1
1106J
1107J
1108 1
1109J
1110J
1111J
1112J
1113J
I
1114|
1115J
1116J
1117J
1118J
1119J
1120 j
1121|
1122|
1123|
1
1124|
1125|
1126|
1127|
1128|
1129|
1130 1
1131 j
1132 1
1133 1
1
1134|
1135J
1136J
1137|
1138 j
1139 j
1140 j
1141J
1142|
1143J
I
1144J
1145J
1H6J
1147]
Vusj
1149|
1150|
1151 1
1152J
1153J
I
I

A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
A|
A|
A|
I
A|
A|
A|
A|
A|
A|
A|
*t
A|
A|
I
I
Scorer
HP |
HP |
HP |
HP |
MP|
HP |
HP |
HP |
HP |
HP|
I
HP|
HP |
HP |
HP |
HP |
HP |
HP |
HP|
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP|
HP)
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
I
Treat.
Code
t
t
t
t
t
t
t
t
t
t

c
c
c
e
c
t
t
t
t
t

t
t
t
t
t
t
t
t
t
t

c
c
c
c
c
t
t
t
t
t

t
t
t
t
t
t
t
t
t
t


Sex
I H
I «l
I «l
I -I
I «l
I «l
I •!
•I
I H
I H
I I
I f|
I f|
I »l
I «l
I f|
I f|
I *l
'I
f|
I *l
I
'I
f|
'I
I 
-------

51
52
53
54
55
56
57
58
59
60

61
62
63
64
65
66
67
68
69
70

71
72
73
74
75
76
77
78
79
80

81
82
83
84
85
86
87
88
89
90

91
92
93
94
95
96
97
98
99
100


chr-some
break
0|
o|
0|
0|
0|
0|
0|
°l
o|
o|
I
o|
o|
0|
o|
o|
o|
o|
0|
0|
°l
I
o|
o|
2|
o|
0|
°l
o|
o|
11
0|
I
°l
o|
o|
0|
°l
0|
o|
o|
o|
o|
I
11
0|
°l
11
°l
°l
o|
o|
°l
"I
1
1
ehr-tid
exchange
°l
°l
°l
°l
°l
o|
°l
°l
°l
0|
1
o|
°l
"1
o|
o|
o|
o|
0|
0|
°l
1
o|
o|
o|
o|
o|
0|
o|
o|
o|
"1
1
o|
o|
o|
0|
0|
o|
°l
o|
0|
o|
1
o|
"1
o|
o|
°l
o)
°l
°l
°l
o|
1
1
chr-some
exchange
°l
°l
0|
"1
o|
°l
°l
o|
0|
°l
1
°l
o|
°l
°l
0|
o|
o|
o|
o|
o|
1
0|
o|
o|
°l
0|
0|
o|
o|
o|
°l
1
0|
0|
°l
o|
o|
o|
o|
o|
"I
o|
1
o|
o|
0|
°l
o|
-ii
01
>l
°l
°l
1
1
other
aberratn
0|
0|
o|
o|
°l
0|
°l
0|
o|
0|
1
°l
0|
0|
o|
0|
°l
0|
0|
o|
°l
1
0|
0|
o|
o|
°l
°l
0|
0|
°l
o|
1
0|
0|
0|
o|
0|
0|
0|
0|
0|
0|
1
0|
0|
o|
o|
°l
0|
0|
0|
0|
»l
1
1
CA/cell
exc gap
0.120|
0.040 |
°l
0.040]
0.120 j
0.240 |
0.080 j
0.040 j
0.120 j
0.040 j
I
0.040)
0.040 j
0.040J
0.040 j
0.040J
0.040 |
0.040|
«l
0.040|
o|
I
0.040|
0.040 j
0.160 j
0.040 |
0|
0.080|
0.080 j
0.040 j
0.160 j
0.120 j
1
0.040J
0.080 j
o|
o|
"I
0.040)
0.080)
0.080J
0.120)
0.120)
1
0.200)
0.120)
o.owj
0.200)
0.040)
0.040 j
0.120)
0)
0.240)
0.120)
I
I
cells w/
0 CA
2* I
24)
25)
24)
24)
24 1
24)
24)
24)
24)
I
24)
24)
24)
24)
24)
24)
24)
25)
24 1
25 1
I
24)
24)
23|
24)
25 1
24)
24)
24)
24)
24)
I
24)
23)
25)
25)
25)
24)
24)
24)
24)
24)
I
22)
24)
24)
24)
24)
24)
24 1
25 1
24)
24)
I
I
cells w/
1 CA
«l
11
"1
M
0|
o|
°l
1)
°l
11
I
I
I
I
I
I
11
11
°l
11
o|
1
11
11
«l
11
o|
0)
0|
11
»l
0|

11
2|
0|
0|
0)
11
0|
0|
o|
»l
1
11
o|
0|
°l
M
1)
0)
0|
0)
o|
I
I
cells w/
2 CA
°l
°l
"I
0|
°l
0|
11
0)
0)
0|
1
0|
0)
0|
o|
o|
0|
0|
"1
°l
o|
1
0|
°l
2|
»l
0|
11
11
°l
o|
11
1
o|
o|
°l
o|
o|
0|
11
11
0|
0|
1
2|
0|
11
o|
0|
0)
o|
°l
o|
"I
1
1
cells w/
3 CA
1|
«l
"1
0|
1|
0|
o|
0)
M
o|
I
"I
0|
o|
»l
«l
o|
0|
o|
0|
o|
I
0|
o|
°l
0|
o|
0|
o|
0)
o|
0|
I
0|
0|
"I
0|
0|
0|
0|
o|
1)
1)
I
o|
11
0|
0|
0|
o|
11
0|
°l
1)
1
1
cells w/
4 CA
»l
0|
o|
0|
«l
0|
0|
°l
"1
°l
1

-------

51
52
53
54
55
56
57
58
59
60

61
62
63
64
65
66
67
68
69
70

71
72
73
74
75
76
77
78
79
80

81
82
83
84
85
86
87
88
89
90

91
92
93
94
95
96
97
98
99
100


cells w/
6 CA
0|
o|
0|
o|
o|
1|
o|
01
o|
"1
1
0|
0|
0|
o|
o|
0|
o|
0|
o|
0|
1
°l
o|
»l
o|
0|
°l
°l
0|
o|
0|
1
°i
0|
o|
"I
0|
0|
0|
0|
0|
o|
1
0|
°l
°l
0|
0|
0|
o|
o|
11
o|
I
I
cells w/
7 CA
o|
0|
0|
0|
o|
o|
0|
0|
o|
0|
I
0|
0|
0|
o|
o|
0|
0|
0|
0|
0|
I
o|
o|
0|
o|
o|
o|
0|
0|
0|
o|
I
°l
0|
o|
0|
0|
0|
o|
o|
«l
o|
I
o|
o|
0|
0|
o|
o|
0|
0|
0|
0|
I
I
cells w/
8 CA
0|
0|
0|
o|
o|
o|
o|
0|
0|
0|
I
0|
o|
o|
0|
0|
0|
o|
°l
«l
o|
I
o|
0|
0|
0|
«l
0|
o|
«l
°l
0|
I
o|
0|
0|
o|
o|
0|
0|
o|
°l
o|
I
°l
o!
"I
°l
0|
0|
0|
0|
°l
°l
I
I
cells u/
9 CA
o|
0|
°l
o|
0|
o|
«l
°l
o|
0|
I
0|
0|
0|
0|
°l
0|
o|
o|
°l
0|
I
0|
0|
°l
0|
o|
o|
0|
0|
«l
°l
I
o|
0|
0|
o|
o|
0|
0|
0|
0|
o|
I
0|
0|
°l
°l
0|
o|
0|
o|
»l
0|


cells M/
10+ CA
°l
0|
0|
0|
0|
0|
0|
°l
0|
o|
I
0|
0|
0|
o|
o|
0|
0|
o|
o|
0|
I
o|
0|
o|
0|
o|
t»l
0|
o|
»l
0|
I
°l
0|
o|
o|
0|
0|
o|
0|
"I
«l
I
o|
»l
0|
°l
0|
o|
0|
0|
o|
0|
I
I
pet. CA
positive
4.0|
4.0|
0.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
I
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
0.0|
4.0|
0.0|
I
4.0|
4.0|
8.0|
4.0|
0.0|
4.0|
4.0|
4.0|
4.0|
4.0|
I
4.0|
8.0|
o.oj
o.oj
o.oj
4.0|
4.0|
4.0|
4.0|
4.0|
I
12. 0|
4.0|
4.0|
4.0|
4.0|
A.0|
4.0|
0.0|
4.0|
4.0|
I
I
HI
# cells
500 1
500 1
500 1
500 j
500 j
500 j
500 j
500 1
500 1
500 j
I
500 1
500 j
500 j
500 j
500 j
500 1
500 1
500 j
500 j
500 1
I
500 1
500 1
500 j
500 j
500 j
500 1
500 j
500 j
500 1
500 j
I
500 1
500 1
500 1
500 j
500 j
500 j
500 1
500 1
500 1
500 1
I
500 1
500 1
500 1
500 1
500 1
SCO |
500 j
500 1
500 1
500 1
I
I
MI
# meta
«|
121
"1
14 1
19|
2|
5|
*l
•I
9|
I
25 1
24 1
23|
26|
32 1
25 1
24 1
26|
25 1
21 1
I
24 1
23|
23|
24 1
25 1
32 1
25 1
21 1
22 1
19|
I
25 1
18|
17|
25 1
32 1
31|
30 1
18|
19|
25 1
1
«|
18|
20 1
30 1
14|
18|
19|
25 1
14|
15|
1
1
HI
percent
3.00|
2.40J
2.20J
2.80|
3. 80 1
0.40 j
i.ooj
0.80J
1.60J
1.80 j
I
5.00|
4. 80 1
4.60J
5. 20 1
6.40J
5. 00 1
4. 80 1
5. 20 j
5.00J
4. 20 1
I
4.80J
4. 60 j
4.60J
4. 80 1
5.00J
6.40J
5. 00 j
4.20J
4. 40 j
3. 80 1
I
5. 00 1
3.60 j
3. 40 1
5. 00 1
6. 40 j
6.20J
6. 00 1
3. 60 1
3. 80 1
5.00|
I
3.00|
3. 60 1
4.00|
6.00|
2. 80 j
3. 60 1
3. 80 1
5. 00 j
2.80|
3. 00 1
I
I
56

-------

101
102
103
104
105
106
107
108
109
110

111
112
•113
114
115
116
117
118
119
120

121
122
123
124
125
126
127
128
129
130

131
132
133
134
135
136
137
138
139
HO

141
142
143
144
145
K6
147
148
149
150
Animal Slide
1154)
1155J
11S6J
1157J
1158J
1159 j
1160J
1161 1
1162 j
1163 1
I
1164 1
1165J
1166 1
1167|
1168 1
1169 j
1170J
1171)
1172)
1173J
1
1174|
1175J
1176 j
1177J
1178|
1179|
1180|
1181|
1182|
1183|
1
1054|
1055 j
1056 j
1057J
1058 j
1059 1
1060 j
1061 j
1062 1
1063 j
I
1064 1
1065 j
1066J
1067|
1068J
1069 1
1070 1
1071 1
1072 1
1073J
I
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A|
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A
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A|
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8|
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B|
B|
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B
B
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B|
B|
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Scorer
MP|
HP |
MP|
MP|
HP |
HP |
HP |
HP |
HP |
HP |
I
HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP|
HP |
HP |
I
HP |
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HP |
HP |
HP |
HP |
HP |
HP |
HP |
HP |
I
BN|
BN|
BN|
BN|
BN|
BN|
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8N|
BN|
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BN|
3N|
BH|
BM|
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I
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Treat.
Code
c
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m
m
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1 o|
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1 
-------

101
102
103
104
105
106
107
108
109
110

111
112
113
1U
115
116
117
118
119
120

121
122
123
124
125
126
127
128
129
130

131
132
133
134
135
136
137
138
139
140

141
142
143
144
145
146
147
148
149
150


chr-some
break
0|
o|
«l
°l
0|
0|
0|
0|
0|
o|
I
o|
°!
0|
°l
o|
0|
0|
"I
11
°l
1
11
o|
o|
11
0|
"I
0|
0|
°l
11
I
"I
o|
0|
"I
0|
»l
°l
o|
0|
0|
I
o|
0|
0|
0|
0|
1]
0|
o|
11
0|
I
I
ehr-tid
exchange
o|
°l
0|
o|
0|
o|
o|
o|
0|
o|
I
o|
o|
o|
0|
0|
o|
°l
0|
0|
0|
I
o|
o|
o|
11
0|
11
*l
o|
2|
°l
I
°l
°l
0|
o|
0|
0|
o|
o|
°l
0|
I
0|
0|
°l
°l
°l
11
0|
o|
o|
o|
1
1
chr-some
exchange
o|
0|
o|
"1
°l
o|
0|
°l
°l
"I
1
°l
"I
0|
°l
°l
0|
0|
°l
0|
0|
1
0|
0|
o|
0|
0|
0|
0|
o|
0|
0|
1
°l
o|
°l
o|
o|
o|
0|
o|
0|
°l
1
0|
0|
o|
o|
o|
0|
°l
0|
0|
o|
1
1
other
aberratn
"I
0|
0|
0|
0|
0|
o|
°l
°l
°l
1
0|
0|
°l
°l
0|
°l
0|
"I
o|
0|
1
0|
0|
0|
o|
0|
o|
o|
0|
o|
0|
1
o|
"1
0|
o|
0|
0|
"1
0|
o|
0|
1
0|
°l
• o|
°l
0|
0|
°l
°l
o|
0|
I
1
CA/cell
exc gap
0.040)
0|
0.120)
0.040 |
0|
0.040)
0.040)
°l
0.080)
0.080)
I
0.120J
0.080)
0.040)
0.080)
0.040)
0.200)
0.040)
0.120)
0.080)
0.200)
I
0.320 |
0.240)
0.160)
0.520)
0.400)
0.400 j
0.280 1
0.160)
0.240)
0.280)
I
0.080)
0.040)
0.800 j
0.080)
0|
0.040)
0.040)
0|
0.080)
0.080)
I
0.080)
0.080)
°l
0.160)
0.080)
0.280)
0.160)
0.080)
0.200)
0.080)
1
1
cells w/
0 CA
24)
25 1
22 1
24 1
25 1
24 1
24 1
25 1
24 1
24)
1
24)
24 1
24 1
24 1
24 1
24)
24)
24)
24)
24 1
1
24 1
24 1
24 1
22 1
23)
23 1
24)
24)
2*1
23 1
1
23)
24 1
5|
23 1
25)
24)
24 1
25 1
24)
24)
1
2*1
24)
25)
24)
24)
23 1
24)
24)
24)
24)
1
1
cells M/
1 CA
M
°l
3|
1|
0|
1|
M
o|
0|
0|
1
o|
o|
11
°l
11
o|
11
0|
0|
o|
I
o|
o|
»l
11
0|
o|
0)
0|
«l
0|
I
2|
M
20)
2|
0)
1|
1|
0|
o|
0|
I
0|
"I
0|
0|
o|
°l
o|
o|
0|
"I
I
I
cells w/
2 CA
°l
"I
0|
o|
"I
«l
o|
o|
1|
11
1
o|
M
0|
1|
»l
o|
o|
o|
11
o|
I
"I
o|
°l
11
o|
11
0|
0|
o|
11
I
0|
"I
0|
o|
0|
0|
o|
0)
11
11
I
11
11
0|
0|
1)
11
0|
11
3 1
11
1
1
cells u/
3 CA
°l
0|
0|
0|
0|
o|
0|
0|
0|
o|
1
1|
«l
"I
o|
o|
0|
o|
11
°l
"1
1
0|
o|
°l
o|
11
o|
o|
"I
"I
"I
I
°l
o|
o|
o|
o|
0|
°l
0|
0|
»l
I
°l
0|
0|
0|
0|
°l
f|
0|
o|
0|
I
I
cells w/
4 CA
o|
°l
0|
0|
o|
0|
o|
«l
«l
«l
I
0|
0|
o|
0|
o|
o|
0|
»l
0|
0|
I
0|
o|
M
°l
0|
0|
0|
11
0|
"I
1
°l
0|
0|
o|
°l
o|
o|
0|
o|
"1
1
°l
o|
0|
M
0|
0|
11
o|
"I
3 1
I
I
cells w/
5 CA
°l
"I
0)
o|
°l
0|
o|
o|
0|
0|
I
0|
»l
0|
0|
o|
11
0|
o|
°l
11
1
»l
0|
"1
0|
o|
0|
0|
0|
o|
11
1
o|
0|
"1
o|
»l
«l
0|
0)
o|
0|
1
o|
o|
0|
0|
0)
11
."I
0|
11
0|
I
I
58

-------

101
102
103
104
105
106
107
108
109
110

111
112
113
114
115
116
117
118
119
120

121
122
123
124
125
126
127
128
129
130

131
132
133
134
135
136
137
138
139
140

141
142
143
144
145
146
147
148
149
150


cells w/
6 CA
0|
"I
°l
0|
o|
°l
o|
o|
°l
o|
I
0|
o|
o|
0|
o|
0|
°l
0|
«l
0|
I
0|
11
o|
o|
0|
o|
o|
o|
11
0|
I
o|
o|
o|
0|
0|
o|
o|
o|
°l
0 1
I
o|
°l
0|
°l
o|
o|
°l
°l
o|
0|
I
I
cell* w/
7 CA
o|
°l
o|
o|
o|
o|
o|
0|
o|
o|
I
°l
o|
o|
°l
0|
o|
o|
°l
°l
o|
I
0|
°l
°l
o|
11
o|
11
°l
o|
o|
I
o|
o|
0|
o|
o|
°!
o|
o|
o|
o|
I
o|
°l
o|
o|
o|
o|
o|
"I
"I
o|
I
I
cells w/
8 CA
°l
"I
o|
o|
0|
o|
o|
o|
0|
o|
I
0|
0|
0|
o|
°l
°l
°l
°l
°l
"I
I
11
0|
0|
°l
°l
11
o|
°l
°l
°l
I
0|
o|
o|
o|
o|
°l
o|
°l
o|
o|
I
°l
°l
°l
°!
0|
°l
0|
0|
0|
0|
I
'
cells w/
9 CA
"I
0|
°l
0|
°l
0|
0|
0|
o|
o|
I
o|
"I
o|
o|
"I
o|
0|
»l
»1
o|
1
o|
0|
0|
o|
o|
0|
0|
o|
»l
o|
1
0|
o|
"1
°l
0|
0|
0|
0|
o|
0|
1
0|
0|
0|
0|
o|
0|
0|
0|
0|
"1
!
i
cells w/
10* CA
o|
0|
0|
0|
0|
o|
o|
0|
o|
"I
I
o|
«l
«l
0|
»l
0|
o|
o|
o|
o|
I
o|
o|
0|
11
o|
"1
0|
0|
0|
"1
1
"I
"I
o|
0|
0|
0|
0|
"1
0|
0|
1
o|
0|
0|
o|
0|
o|
o|
0|
0|
0|
1
1
pet. CA
positive
4.0|
0.0|
12. 0|
4.0|
o.oj
4.0|
4.0|
o.oj
4.0|
4.0|
I
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
4.0|
I
4.0|
4.0|
4.0|
12. 0|
8.0J
8.0J
4.0|
4.0|
4.0|
8.0|
I
8.0|
4.0|
80. 0|
8.0J
0.0|
4.0|
4.0|
0.0|
4.0|
4.0|
I
4.0|
4.0|
0.0|
4.0|
4.0|
8.0|
4.0|
4.0|
4.0)
4.0|
I
I
MI
# cells
500 1
500 1
500 j
500 j
500 j
500 1
500 j
500 j
500 j
500 j
I
500 1
500 j
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 1
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500)
I
500 1
500 1
500 1
500 1
500 j
500 1
500 1
500 j
500 1
500 1
I
I
NI
# met a
12|
15|
14|
25 1
24 1
21 1
25 1
15|
16|
25 1
I
28|
24 1
25 1
26|
28 1
24 1
25 1
26|
32 1
19|
I
«|
32 1
15|
28 1
24 1
23 1
25 1
25 1
21
25 1
I
25 1
24 1
32 1
33 1
15|
15|
25 1
24 1
26 1
23|
I
25 1
14|
25 1
23 1
25 1
32 1
18|
17|
16|
«|


NI
percent
2.40|
3.00J
2.80J
5.00J
4.80|
4.20J
5.00J
3.00J
3. 20 j
s.ooj
1
5.60|
4.80J
5. 00 1
5. 20 j
5. 60 j
4. 80 j
s.ooj
5.20J
6. 40 j
3.80J
1
3.00|
6. 40 j
3.00J
5.60J
4. 80 j
4.60J
s.ooj
s.ooj
4.20J
s.ooj
1
5.00|
4.80J
6.40J
6.60J
3. 00 j
3.00J
S.OOj
4.80J
5.20J
4.60J
1
5.00|
2.80|
s.ooj
4.60|
s.ooj
6.40|
3.60|
3.40|
3.20|
3.00J
I
1
59

-------
Animal    Slide
Scorer    Treat.
          Code
                                       Sex
                                                 Dose
Sample    Number    chr-tid   chr-some  chr-tid
                                                           Time
                                                                     Cells
                                                                                                  break
151
152
153
154
155
156
157
158
159
160

161
162
163
164
165
166
167
168
169
170

171
172
173
174
175
176
177
178
179
180

181
182
183
184
185
186
187
188
:89
190

191
192
193
194
195
196
197
198
199
200


1074 1
1 1075|
1076|
1077)
1078J
1079 j
1080J
1081 1
1082J
1083 1
1
1084|
1085 j
1086J
1087J
1088 j
1089J
1090J
1091 j
1092J
1093J
I
1094 1
1095|
1096|
1097|
1098 j
1099J
1100|
noi|
1102|
1103|
1
1104|
1105|
1106)
1107|
1108|
1109)
1110J
111! |
1112|
1113J
I
11 14 1
1115|
1116|
1117)
1118J
1119|
1120]
1121|
1122|
1123)
I
I
B|
B|
B|
B|
•I
B|
B|
B|
B|
•I
I
B|
B|
B|
B|
B|
B|
•I
•I
•I
B|
I
B|
B|
B|
•I
B|
B|
B|
B|
•I
B|
I
•I
B|
•I
B|
•I
B|
•I
B|
B|
B|
I
•I
B|
•I
B|
B|
3|
B|
B|
B|
B|
I
I
BN|
BN|
BN|
BN|
BN|
BH|
BN|
BN|
BN|
BM|
I
BN|
BN|
BN|
BN|
BN|
BN|
BK|
BN|
BN|
BN|
I
BN|
BN|
BNJ
8N|
BN|
BH|
BN|
BN|
BN|
BN|
I
BN|
BN|
BN|
BN|
BN|
BN|
BN|
8N|
BN|
BN|
I
BN|
BN|
BK;
BN|
BN|
BN|
8M|
BN|
BN|
BN|
I
I
C
c
c
c
c
t
t
t
t
tl
I
tl
tl
tl
tl
tl
tl
t
tl
t
t

c
c
c
c
c
tl
tl
tl
t
tj
I
tl
tl
tl
tl
tl
tl
tl
t
tl
tl

c
c
c
e|
c
tl
t
t
t
t


•I
m|
•I
•I
•I
•I
•I
"I
•I
•I
I
•I
•I
•I
•I
•I
m|
•I
•I
•I
•I
I
•I
m|
•I
•I
•I
•r
"I
m|
•I
m|
I
•I
•I
•I
•I
•I
•I
•I
m|
"I
"I
I
'I
'I
'I
'I
f|
'I
'I
M
'I
f|
I
I
Q I
Q |
o|
o|
0|
500 1
500 j
500 j
500 1
500)
I
1000)
1000)
1000 j
1000)
1000)
2000)
2000 j
2000 1
2000)
2000)
I
o|
0|
0|
°l
0|
500 1
500 1
500 j
500)
500 1
I
1000)
1000 j
1000 1
1000J
1000 j
2000)
2000 1
2000)
20CO|
2000)
1
0|
o 1
Q j
o|
0|
500 1
500 1
500)
500 1
500 1
1
1
48 1
48 1
48|
48 1
48 1
48 1
48|
48|
48|
"1
1
48|
48 1
48 1
48|
48 1
48 1
48|
48 1
48 1
48 1
1
72 1
72|
72|
72 1
72 1
72 1
72 1
72 1
72|
72|
1
72 1
72 1
-2|
72|
72|
72 1
72|
721
721
72 1
1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
24 1
1
1 	
25 1
25 1
25 1
25 1
25|
25 1
25 1
25 1
25 1
25 1
1
25 1
25 1
25 1
25 1
25 1
25 1
25|
25 1
25 1
25 1
1
25 1
25 1
25 1
25|
25 1
25 1
25 1
25 1
25 1
25 1
1
25 1
25 1
25|
25 1
25 1
25 j
25 1
25 1
25 1
25 1
1
25|
25 1
25 1
25 1
25 1
25 1
25 1
25)
25 1
25 1
1
1
o|
o|
0|
1|
o|
o|
0|
o|
3|
o|
I
o|
4|
o|
o|
3|
o|
3|
o|
o'l
4|
I
o|
o|
o|
o|
3|
0|
o|
o|
°l
0|
I
o|
o|
o|
0|
2|
o|
o|
oi
o|
0|
I
2|
0|
o|
°l
0|
0|
0|
0|
o|
3|
I
I
0|
0|
o|
0|
o|
°l
o|
0|
o|
0|
I
o|
o|
0|
0|
o|
"I
o|
0|
0|
0|
I
0|
o|
0|
0|
o|
0|
o|
0|
o|
o|
I
o|
o|
0|
0|
0|
o|
»l
o|
a|
o|
I
0|
oi
o|
0|
o|
o|
o|
0|
o|
°l
I
I
2|
o|
11
11
11
3|
4|
5|
"I
2|
I
4|
5|
•I
3|
0|
10|
2|
7|
3|
7|
I
1|
2|
o|
1|
1|
1|
1|
2|
o|
2|
I
2|
3|
4|
°l
2|
1|
6|
2|
5|
5|
I
2|
o|
M
M
1|
OI
0|
11
4|
'1
1
I
                                                   60

-------

151
152
153
154
155
156
157
158
159
160

161
162
163
164
165
166
167
168
169
170

171
172
173
174
175
176
177
178
179
180

181
182
183
184
185
186
187
188
189
'90

191
192
193
194
195
196
197
198
199
200

chr-some
break
o|
0|
0|
o|
0|
0|
o|
o|
o|
o|
I
»l
1|
11
0|
0|
M
o|
0|
o|
0|
I
0|
o|
o|
o|
o|
o|
o|
o|
°l
0|
I
°l
°l
2|
0|
o|
0|
11
»l
11
0|
I
0|
0|
0|
°l
°l
°l
0|
0|
»!
0|
I
I
chp-tid
exchange
0|
0|
o|
o|
o|
0|
"I
11
°l
o|
I
o|
o|
11
o|
3|
1|
°l
11
3)
2|
I
o|
o|
o|
"I
"I
o|
°l
o|
0|
°l
I
0|
"I
11
o|
o|
0|
0|
0|
°l
0|
I
°l
°!
0|
0|
0|
o|
°l
0|
°l
0|
I
I
chr-some
exchange
0|
0|
0|
0|
°l
°l
0|
o|
0|
o|
I
0|
°l
0|
0|
o|
"I
o|
0|
°l
0|
I
0|
°l
o|
°l
o|
o|
o|
o|
o|
°l
I
o|
o|
0|
0|
0|
o|
0|
«l
°l
0|
I
0|
0|
0|
0|
o|
3|
0|
0|
0|
0|
I
I
other
aberratn
°l
0|
°l
o|
«l
0|
0|
0|
0|
°l
I
0|
0|
0|
0|
°l
0|
0|
0|
0|
0|
I
o|
0|
o|
0|
0|
o|
°l
0|
0|
0|
I
o|
0|
0|
o|
0|
3|
o|
o|
0|
o|
I
0|
0|
0|
0|
0|
0|
0|
o|
o|
0|
I
CA/cell
exc qao
0.080)
o|
0.040)
0.040 j
0.040 |
0.120 |
0.160)
0.240J
0|
0.080 |
I
0.160 |
0.240 j
0.400 j
0.120 j
0.120 |
0.480J
0.080J
0.320 j
0.240J
0.360)
1
0.040)
0.080 j
0|
0.040)
0.040 j
0.040J
0.040J
0.080J
0|
0.080|
1
o.oso)
0.120J
0.280 |
o|
0.080 |
0.040 |
0.280)
o.osoj
0.240 j
0.200]
I
o.osoj
0|
3.040|
0.040|
0.040 |
°l
0|
0.040)
0.160)
0.040)
1
1
cells w/
0 CA
23 1
25)
2*1
24)
2*1
2*1
2*1
23 1
25)
2*1
1
2*1
23)
23)
2*1
24 1
22)
2*1
23 1
23)
22)
1
2*1
23)
25 1
2*1
2*1
2*1
24)
2*1
25 1
2*1
1
2*1
2*1
23)
25)
2*1
2*1
22)
2*1
21)
24 1
1
23)
25)
2*1
24 1
24 1
25 1
25)
24)
23|
2*1
1
1
cells w/
1 CA
2|
°l
1|
M
1|
0|
0|
°l
0|
°l
1
o|
"1
0|
o|
°l
o|
°l
0)
0)
o|
1
11
2|
0|
1|
1|
1|
M
o|
0|
°l
1
0|
o|
°l
°l
o|
11
0|
»l
2|
°l
I
2)
0|
'I
M
1|
0|
0|
1|
0|
1|
I
!
cells w/
2 CA
0|
0|
°l
»l
°l
0|
o|
1|
o|
1|
1
°l
1|
0|
o|
0)
°l
1|
1|
o|
o|
1
0|
o|
o|
0|
"I
0|
o|
M
"1
11
1
11
o|
1)
o|
1)
°l
M
11
2)
o|
I
0|
0|
0|
0)
0|
0|
0|
0|
2|
0|
I
I
cells w/
3 CA
°l
0|
"I
0|
0|
1|
o|
0|
o|
0|
I
"I
0|
11
M
1|
1|
0|
"I
2|
3|
1
0|
0|
0|
0|
0|
0|
0|
0|
o|
0|
1
"1
11
o|
o|
o|
»l
2|
°l
0|
0|
1
°l
o|
0|
"1
0|
0|
o|
«l
o|
o|
1
1
cells w/
4 CA
o|
o|
0|
o|
0|
0|
11
11
»l
o|
I
11
11
o|
o|
0|
M
0|
"1
0|
o|
1
o|
0|
o|
0|
0|
o|
0|
o|
"I
o|
1
0|
0|
"1
"1
o|
o|
0|
0|
0|
o|
1
°l
o|
0|
o|
o|
0|
o|
0|
0|
0|
1
1
cells w/
5 CA
o|
°l
0|
0|
"1
°l
0|
"1
«l
«l
1
°l
o|
o|
0|
0|
M
o|
0)
0|
o|
1
o|
o|
»l
°l
0|
o|
0|
0|
o|
0|
1
"1
»l
11
"I
o|
0|
0|
0|
o|
11
1
0|
o|
0|
0|
0|
o|
o|
°l
o|
0|
1
!
61

-------

151
1S2
153
154
155
156
157
158
159
160

161
162
163
164
165
166
167
168
169
170

171
172
173
174
175
176
177
178
179
180

181
182
183
184
185
186
187
188
189
190

191
192
193
194
195
196
197
198
199
200


cells w/
6 CA
0|
o|
o|
0|
°l
0|
o|
0|
»l
0|
1
°l
"1
0|
o|
°l
°l
»l
1|
o|
0|
1
o|
°l
o|
°l
0|
0|
0|
o|
"1
0|
1
o|
o|
o|
01
«l
0|
°l
0|
o|
«l
1
0|
0|
0|
0|
0|
°l
0|
o|
0|
0|
1
!
cells u/
7 CA
o|
o|
o|
o|
0|
o|
o|
0|
o|
°l
1
"1
o|
11
o|
o|
"I
o|
0|
0|
°l
1
o|
°l
0|
°l
"I
0|
o|
0|
0|
0|
1
0|
of
0|
o|
°l
0|
0|
o|
0|
0|
1
0|
0|
0|
0|
o|
0|
o|
o|
°l
o|
1
1
cells u/
8 CA
°l
0|
»l
°l
0|
o|
o|
o|
o|
0|
1
0|
o|
o|
o|
"1
0|
0|
°l
o|
o|
1
o|
o|
0|
o|
0|
°l
0|
°l
0|
0|
1
0|
o|
0|
0|
°l
0|
o|
o|
of
°l
1
0|
0|
0|
°l
°l
0|
0|
o|
0|
0|
1
1
cells u/
9 CA
0|
°l
0|
o|
°l
"1
0|
o|
0|
0|
1
o|
o|
°l
o|
0|
»l
o|
o|
o|
o|
1
"1
0|
0|
0|
0|
o|
o|
o|
0|
0|
1
o|
0|
o|
o|
0|
0|
°l
°l
0|
°l
1
o|
0|
°l
°l
o|
0|
o|
°l
0|
0|
1
1
cells w/
10+ CA
°l
o|
o|
0|
o|
°l
°l
0|
°l
"1
1
o|
0|
0|
o|
0|
"1
0|
o|
0|
o|
1
°l
0|
0|
o|
°l
«l
0|
0|
"I
o|
1
0|
0|
0|
o|
0|
o|
0|
o|
"1
o|
1
0|
0|
0|
o|
0|
0|
0|
o|
0|
0|
1
I
pet. CA
positive
8.0|
o.oj
4.0|
4.0|
4.0|
4.0|
4.0|
8.0|
0.0|
4.0|
1
4.0|
a.o|
B.0|
4.0|
4.0|
12. 0|
4.0|
8.0|
8.0J
12. OJ
1
4.0|
8.0)
o.oj
4.0|
4.0|
4.0|
4.0|
4.0|
0.0|
4.0|
1
4.0|
4.0|
8.0|
o.oj
4.0|
4.0|
12.0|
4.0|
16. 0|
4.0|
I
8.0|
3.0|
4.0|
4.0|
4.0|
0.0|
o.oj
4.0|
8.0|
4.0|
I
I
MI
# cells
500 1
500 j
500 j
500 j
500 j
500 j
500 1
500 j
500 j
500 1
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 1
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 j
50CI
500 1
500 j
500 1
500 j
I
I
MI
» meta
25 1
35 1
32 1
24 1
26 1
«l
18|
17|
16|
14|
1
12|
10|
15|
18|
10|
9\
5|
21
1|
B|
I
25 1
26|
24 1
25 1
28|
32 1
15|
24 1
25 1
«|
I
25 1
15|
14|
«|
12)
"I
12|
13|
10|
2|
1
25 1
32 1
25 1
23 1
21 1
24 1
25 1
25 1
25 1
21 1
I
I
MI
percent
5. 00 1
7. 00 1
6.40J
4.80J
5. 20 1
3. 00 1
3. 60 1
3.40J
3.20J
2.80J
I
2.40|
2.00J
3.00J
3.60J
2.00J
1.80 1
1.00J
0.40J
0.20 1
1.60 j
I
5.00|
5.20J
4.80J
s.ooj
5.60J
6.40J
3. 00 j
4.80J
s.ooj
3.00J
I
5.00|
3. 00 1
2.80J
3.00J
2.40J
2.20J
2.40J
2.60J
2.00J
0.40J
I
5.00J
6.40J
5. 00 1
4.60|
4.20J
4.80|
S.OOj
s.ooj
S.OOj
i.20|
I
I
62

-------

201
202
203
204
205
206
207
208
209
210

211
212
213
2H
215
216
217
218
219
220

221
222
223
224
225
226
227
228
229
230

231
232
233
234
235
236
237
238
239
240

241
242
243
:44
245
246
247
248
249
250


Animal Slide
1124 1
1125 j
1126)
1127J
1128 j
1129 j
1130 j
1131J
1132 j
1133J
I
1134 1
1135 j
1136 j
1137 1
1138 j
1139)
1140 j
1141J
1142 1
1143 1
I
1144)
1145|
1146|
1147|
1148|
1149|
1150|
1151J
1152|
1153|
1
1154|
1155|
1156|
1157|
1158 j
1159 1
1160 1
1161 j
1162 1
1163J
I
1164)
1165|
1166J
1167 1
1168J
1169|
1170 1
1171|
1172|
1173|
I
I

•I
•I
•I
B|
B|
•I
B|
•I
•I
•I
I
8|
B|
B|
B|
•I
B|
B|
•I
B|
B|
I
B|
B|
•I
B|
B|
B|
•I
B|
•I
B|
I
B|
B|
•I
•I
B
•I
B
B
B
B|
I
•I
B|
B|
B|
B|
B|
B|
•I
•I
B


Scorer
BN|
8N|
8N|
BN|
BN|
BN|
BN|
BN|
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BN|
I
BN|
BN|
BN|
BN|
BN|
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BH|
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BN|
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BNJ
BN|
BN|
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BN|
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BN|
I
I
Treat.
Code
t
t
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t
t
t
t
t
t

c
c
c
c
c
t
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t
t
t

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t
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Sex
I f|
I f|
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I *l
I f|
I f|
I f|
I f|
I f|
I I
f|

-------

201
202
203
204
205
206
207
208
209
210

211
212
213
214
215
216
217
218
219
220

221
222
223
224
225
226
227
228
229
230

231
232
233
234
235
236
237
238
239
240

241
242
243
244
245
246
247
248
249
250

chr-some
break
o|
°l
°l
o|
°l
1|
0|
°l
o|
o|
I
°l
o|
o|
0|
o|
°l
°l
0|
o|
o|
I
11
o|
0|
o|
M
11
o|
0|
o|
11
I
°l
o|
°l
0|
°l
o|
o|
0|
0|
o|
I
11
o|
°l
o|
°l
11
°l
°l
o|
o|
I
I
ehr-tid
exchange
o|
°l
o|
11
°l
0|
o|
o|
0|
11
1
"1
°l
o|
o|
o|
°l
o|
°l
o|
0|
1
°l
o|
0|
o|
11
0|
o|
o|
o|
0|
I
°l
0|
°l
o|
°l
°l
°l
°l
o|
°l
I
°l
0|
°l
o|
°l
°l
°l
°l
0|
2|
I
I
chr-some
exchange
o|
o|
o|
°l
°l
o|
°l
"I
0|
0|
I
"I
°l
0|
o|
°l
"I
°l
"I
o|
o|
I
°l
o|
0|
o|
o|
0|
o|
°l
o|
0|
I
°l
°l
"I
o|
o|
°l
°l
0|
o|
o|
I
°l
°l
°l
°l
0|
°l
0|
°l
o|
o|
I
I
other
aberratn
°l
0|
°l
o|
0|
o|
°l
o|
0|
°l
I
°l
o|
0|
"I
°l
o|
°l
o|
"I
0|
I
0|
o|
o|
o|
o|
0|
0|
°l
o|
o|
I
0|
0|
0|
°l
o|
°l
°l
o|
o|
o|
I
°l
°l
o|
.'!
0|
°l
0|
0|
o|
°l
I
I
Ok/cell
exc gap
0.040]
0.040 j
0.080 |
0.360 j
0.040 j
0.080 |
0.120 |
0.160 |
0.120)
0.080 |
I
. 0.120)
0|
o|
0.040)
°l
0.080)
0.040 j
o|
0.080 |
0.040)
I
0.080)
0.040)
0.080)
0.120)
0.120)
0.240 |
0.160)
0.200 |
o|
0.240)
I
0)
0.080)
o|
0.080)
0.040 1
0.040)
0.040)
0.040)
0.080)
°l
I
0.120)
0.120)
0.120)
0.040)
01
0.240)
0.120)
0.040)
0.160)
0.iOOJ
I
I
cells w/
0 CA
24 1
24 1
24 1
22 1
24 1
24 1
24)
24)
24 1
24 1
I
22 1
25 1
25 1
24 1
25 1
23)
24 1
25 1
24 1
24 1
I
24)
24 1
24 1
24 1
24)
23)
24)
24 1
25 1
24)
I
25 1
23|
25 1
23|
24)
24)
24 1
24)
24)
25)
I
24)
24)
24)
24)
25 1
24)
24)
24)
23|
24 1
I
I
cells w/
1 CA
1|
1|
0)
°l
1|
°l
»l
o|
0|
o|
I
3|
o|
o|
1|
o|
2)
1|
o|
0|
1|
I
0)
II
0|
o|
o|
o|
"1
°l
o|
o|
1
o|
2|
o|
21
1)
1|
1|
11
0|
o|
I
o|
o|
0)
1|
o|
°l
o|
1|
1|
o|
I
I
cells u/
2 CA
°l
o|
M
°l
o|
M
o|
0|
o|
M
I
o|
o|
0|
o|
o|
o|
o|
o|
M
°l
I
1|
o|
11
o|
o|
°l
o|
o|
o|
o|
I
0)
0|
"I
°l
0|
o|
o|
0)
11
o|

o|
0)
°l
0|
0|
°l
0)
0|
o|
o|

cells w/
3 CA
0|
0|
°l
3|
0|
"I
11
o|
11
"I
I
o|
o|
o|
o|
0|
"I
o|
°l
o|
0|
I
o|
o|
0|
11
M
2|
o|
"I
o|
°l
1
°l
o|
o|
o|
o|
o|
o|
o|
0|
°l
1
11
1)
11
0|
°l
o|
11
0)
11
0|
I
I
cells w/
4 CA
0)
"I
°l
"I
0)
°l
o|
1|
0|
o|
I
"I
0|
o|
o|
o|
"I
0)
o|
o|
°l
I
°l
o|
0|
o|
o|
°l
11
0|
o|
o|
I
0|
o|
0|
0|
°l
o|
. o|
0|
°l
°l
I
o|
o|
0)
°l
o|
"I
o|
°l
°l
o|
I
I
cells y/
5 CA
o|
°l
o|
o|
o|
°l
o|
o|
o|
o|
I
°l
o|
o|
0|
o|
0|
o|
0|
ol
o|
I
o|
o|
0)
o|
o|
o|
o|
11
o|
o|
I
0|
0|
o|
o|
o|
o|
o|
o|
0|
o|
I
o|
o|
0)
°l
o|
o|
0|
0|
o|
M
I
I
64

-------

201
202
203
204
205
206
207
208
209
210

211
212
213
214
215
216
217
218
219
220

221
222
223
224
225
226
227
228
229
230

231
232
233
234
235
236
237
238
2J9
240

241
242
243
244
245
246
247
243
249
25 0


cells w/
6 CA
»l
0|
o|
°l
°!
o|
o|
o|
o|
"I
1
o|
o|
o|
0|
o|
0|
o|
0|
°l
o|
1
0|
°l
°l
"1
0|
"I
o|
"1
0|
11
1
0|
01
0|
0|
0|
0|
0|
0|
o|
Of
1
0|
01
°l
0|
•M
11
0|
0|
«l
0|
1
1
cells w/
7 CA
01
0|
0|
0|
o|
»l
°l
0|
o|
"1
1
o|
»l
0|
o|
0|
°l
0|
0|
0|
o|
1
0|
o|
°l
o|
°l
°l
°l
o|
o|
o|
1
o|
°l
°l
0|
0|
0|
°l
°l
0|
o|
1
3|
0|
°l
°!
31
1
°l
°l
°l
0|
°l
1
1
cells w/
8 CA
«l
0|
0|
°l
o|
0|
°l
°l
°l
°l
I
o|
o|
o|
o|
o|
0|
0|
0|
o|
°l
1
o|
o|
0|
0|
0|
o|
o|
o|
»l
o|
1
o|
°l
o|
o|
0|
°l
0|
0|
o|
°l
1
o|
o|
0|
01
0|
°l
0|
o|
0|
o|
1
1
cells u/
9 CA
o|
o|
01
"1
0|
0|
0|
»l
«l
0|
1
0|
"I
o|
°l
»l
0|
«l
0|
0|
°l
1
0|
o|
o|
o|
o|
o|
o|
»l
0|
o|
1
o|
o|
0|
0|
o|
0|
0|
0|
0|
»l
1
0|
o|
0|
0|
o|
"I
o|
"1
0|
o|
1
1
cells w/
10+ CA
o|
°l
0|
0|
0|
o|
o|
0|
0|
0|
I
0|
o|
o|
"I
0|
0|
o|
0|
o|
0|
I
0|
o|
o|
0|
»l
"I
o|
°l
0|
o|
I
0|
°l
o|
0|
0|
0|
°l
0|
o|
°l
I
0|
»l
0|
0|
0|
o|
0|
0|
o|
0|
I
I
pet. CA
positive
4.0|
4.0|
4.0|
12. 0|
4.0J
4.0|
4.0J
4.0|
4.0|
4.0|
I
12. 0|
o.oj
o.oj
4.0|
0.0|
8.0J
4.0|
0.0|
4.0|
4.0|
I
4.0|
4.0|
4.0|
4.0|
4.0|
a.o|
4.0|
4.0|
0.0|
4.0|
I
0.0|
s.oj
o.oj
8.0J
4.0|
4.0|
4.0|
4.0|
4.0|
o.oj
I
4.0|
4.0|
4.0|
4.0|
0.0|
4.0|
4.0|
4.0|
8.0|
4.0|
I
I
MI
# cells
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
500 j
I
500 1
500 j
500 j
500 j
500 1
500 j
500 j
500 1
500 j
500 j
I
I
MI
# meta
20 1
30 1
21 1
25 1
21 1
25 1
32 1
14|
18|
19|
I
15|
19|
32|
35 1
35 1
«|
25 1
24 1
28|
24 1
1
26 1
27|
42|
15|
25 1
32|
25 1
32 1
15|
24 1
I
25 1
26 1
24 1
25 1
32 1
32 1
21 1
20 1
19|
18|
1
20 1
25 1
21 1
24 1
19|
18|
25 1
26 1
15|
14|
1
1
MI
percent
4.00J
6.00J
4.20J
5.00J
4. 20 j
5.00J
6.40J
2.80J
3.60J
3.80J
1
3.00|
3.80J
6.40J
7.00J
7.00J
3.00J
5. 00 j
4. 80 j
5. 60 j
4.80|
1
5.20|
5. 40 j
8.40J
3.00J
s.ooj
6. 40 j
s.ooj
6. 40 j
3.00J
4.80J
1
s.ooj
5.20J
4.80J
5. 00 1
6.40J
6.40J
4.20|
4.00|
3.80J
3.60J
1
4.00|
5.00|
4.20|
4.80|
3.80|
3.60|
5.00|
5.20|
3.00|
2.80|
1
1
65

-------
251
252
253
254
255
256
257
258
259
260
Animal Slide Scorer Treat. Sex Dose Sample Number chr-tid chr-some chr-tid
Code Time Cells qao aao break
1174J
1175J
1176J
1177)
1178J
1179J
1180 1
1181 1
1182 1
1183 j
I
I
B| BN|
B| BN|
B| BN|
B| BN|
B| BN|
B| BH|
B
B
B
B


BN|
BN|
BN|
BN|
I
1
Pi
Pi
Pi
Pi
Pi
Pi
Pi
Pi
Pi
Pi
1
1
m| 12. S|
m| 12. 5|
mj 12. 5|
m| 12.5|
m| 12.5|
f| 12.5|
fj 12. 5|
fj 12.5J
fj 12. 5|
fj 12. 5J
I I
I I
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48 1
48|
48 1
I
t
25 1
25 1
25 1
25)
25 1
25 1
25 1
25 1
25 1
25 1
I
I
0|
3|
°l
1|
0|
0|
5|
0|
0|
4I
1
1
o|
0|
o|
0|
0|
°l
Q I
Q 1
0|
0|
1
1
'1
n
5|
0|
M
4|
3|
7|
5|
5|
1
1
                                                           66

-------

251
252
253
254
255
256
257
258
259
260


chr-some chr-tid chr-some
break exchange exchange
2| 3| 0|
3| 1| 0|
1| 0| 0|
0| 0| 0|
0| 0| 0|
1| 0| OJ
0[ 0| 0|
0| 0| 0|
o| oj o|
0| 0| 0|
I I I
I I '
other CA/cell
aberratn exc gap
0| 0.560]
OJ 0.440]
OJ 0.240J
0| OJ
OJ 0.160J
OJ 0.200]
0| 0.120]
OJ 0.280]
OJ 0.200J
OJ 0.200J
I I
I I
cells w/ cells w/
0 CA 1 CA
20] 0
22] 0
24] 0
25 j 0
24] 0
24] 0
24 j 0
23 j 0
24 j 0
24] 0
I
I
cells w/ cells w/ cells
2 CA 3 CA 4 CA
3| 0|
1| 0|
0| 0|
0| 0|
0| 0|
0| 0|
0| 1|
0| 1|
0| 0|
0| 0|
I
I
w/ cells w/
5 CA
2| 0|
1| 1]
0| 0|
0| 0|
1| 0|
0] 1]
0| 0|
1 0|
1| 0|
0| 1|
I
I
67

-------
cells u/ cells u/ cells w/ cells w/ cells w/ pet. CA
6 CA 7 CA 8 CA 9 CA 10* CA positive *
251
252
253
254
2SS
256
257
258
259
260


°l
0|
1|
o|
°l
o|
o|
o|
o|
0|
I
I
o|
0|
0|
o|
o|
o|
"I
o|
°l
0|
I
I
°l
0|
o|
o|
o|
"I
"I
°l
o|
o|
I
I
0|
0|
°l
0|
o|
0|
0|
o|
o|
o|
I
I
°l
o|
o|
o|
o|
0|
°l
0|
o|
0|
I
I
20.0 1
12. 0|
«.0|
0.0|
4.0|
4.0|
4.0|
8.0|
4.0|
4.0|
I
I
MI HI HI
cells # met a percent
500 1
500 1
500)
500 1
500 1
500 j
500 j
500 1
500 1
500 1
I
I
15|
25 1
32 1
32 1
25 1
24 1
26 1
28|
29 1
27|
1
1
3.00|
5. 00 j
6.40|
6.40J
5. 00 1
4.80J
S.20|
5.60J
5.80J
5. 40 j
1
1
68

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)
  There are 2 outliers at the .05 significance level.

       Line    Dose  Time  Sex       Pet.     Mean Pet.
   1       3     0.0  24.0    m     60.000        18.400
   2     133     0.0  24.0    m     80.000        20.000
                                      69

-------
filename:  C:\CA\CATEST02.ILS   date:              time:
    Endpoint:  Chromosomal aberration (pet. damaged cells)

  Variance inflation factor:     1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer            7.945    24   0.9991
  Sex              7.641    12   0.8125
  Time              3.116     8   0.9269
                                     70

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
24.00 (Males)  for 067889


        0.486
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet.  damaged  cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
13
6
8
13
Cells
Scored
200
250
250
250
Percent
Aberrant
6.5000
2.4000
3.2000
5.2000
SEM
(for Obs)
1.0522
0.6532
0.5333
0.8537
Pairwise
Significance

0.9842
0.9504
0.7215
Time =    48.00 (Males)  for 067889
   P
   alpha
        0.095
        0.040 (scaled by .8, assuming next run to exclude high  dose)
   One tailed test (binomial) for chromosomal aberration  (pet.  damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
12
9
11
17
Cells
Scored
250
250
250
250
Percent
SEM
Pairwise
Aberrant (for Obs) Significance
4.8000
3.6000
4.4000
6.8000
0.8000
0.7180
0.7180
1.0414

0.7482
0.5845
0.1694
                                     71

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
72.00 (Males)  for 067889

        0.110
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
11
7
9
15
Cells
Scored
250
250
250
250
Percent
Aberrant
4.4000
2.8000
3.6000
6.0000
SEM
(for Obs)
0.7180
0.6110
0.7180
1.3663
                                                           Pairwise
                                                       Significance

                                                             0.8315
                                                             0.6760
                                                             0.2102
Time
   P
   alpha
24.00 (Females)  for 067889

        0.381
   =    0.040  (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)
Aberrant
MG/KG
0.00
fJOO.OO
1000.00
2000.00
Cells
10
7
12
10
Cells
Scored
250
250
250
250
Percent
SEM
Aberrant (for Obs)
4.0000
2.8000
4.8000
4.0000
0.5963
0.8537
0.9978
0.0000
                                                           Pairwise
                                                       Significance

                                                             0.7704
                                                             0.3314
                                                             0.5000
                                     72

-------
filename: C:\CA\CATEST02.ILS   date:
                                               time:
Time
       48.00 (Females)  for 067889
   P
   alpha
               0.348
               w • ^ ~m ^
               0.040 (scaled by .8, assuming next run to exclude high dose)

One tailed test (binomial) for chromosomal aberration (pet. damaged cells)
Aberrant
MG/KG
0.00
500.00
1000.00
2000.00
Cells
7
10
12
9
Cells
Scored
250
250
250
250
Percent
Aberrant
2.8000
4.0000
4.8000
3.6000
SEM
(for Obs)
1.3400
0.5963
0.8000
0.7180
                                                           Pairwise
                                                       Significance

                                                             0.2296
                                                             0.1211
                                                             0.3057
Time
   P
   alpha
       72.00 (Females)  for 067889

               0.348
          =    0.040  (scaled by .8, assuming next run to exclude high dose)
   One tailed test (binomial) for chromosomal aberration  (pet. damaged  cells)

MG/KG
0.00
500.00
1000.00
2000.00
Aberrant
Cells
10
8
9
11
Cells
Scored
250
250
250
250
Percent
Aberrant
4.0000
3.2000
3.6000
4.4000
SEM
(for Obs)
1.3333
0.5333
0.4000
0.4000
                                                           Pairwise
                                                       Significance

                                                              0.6844
                                                              0.5925
                                                              0.4118
                                     73

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)
  There are 2 outliers at the .05 significance level.

       Line    Dose  Time  Sex       Pet.     Mean Pet.
   1      3     0.0  24.0    m     60.000        18.400
   2    133     0.0  24.0    m     80.000        20.000
                                    74

-------
filename:  C:\CA\CATEST02.ILS   date:              time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)

  Variance inflation factor:     1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer            7.945    24   0.9991
  Sex              7.641    12   0.8125
  Time              3.116     8   0.9269
                                    75

-------
filename: C:\CA\CATEST02.ILS   date:              time:


Both sexes (All times)  for 067889

   p         =    0.062
   alpha     =    0.040 (scaled by .8, assuming next run to exclude high dose)

   One tailed test (binomial)  for chromosomal aberration (pet. damaged cells)
           Aberrant      Cells    Percent        SEM       Pairwise
   MG/KG      Cells     Scored   Aberrant  (for Obs)   Significance
    0.00         63       1450     4.3448     0.4207
  500.00         47       1500     3.1333     0.2705         0.9587
 1000.00         61       1500     4.0667     0.2929         0.6467
 2000.00         75       1500     5.0000     0.3636         0.1998
                                     76

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (pet.  damaged cells)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex       Pet.     Mean Pet.
   1      3     0.0  24.0    m     60.000        18.400
   2    133     0.0  24.0    m     80.000        20.000
                                     77

-------
filename: C:\CA\CATEST02.ILS   date:
time:
Time =    48.00 (Males)  for 067889

   alpha     =    0.050

   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)
     (with positive controls)
           Aberrant      Cells    Percent        SEM
   MG/KG      Cells     Scored   Aberrant  (for Obs)
    0.00         12        250     4.8000     0.8000
   12.50         18        250     7.2000     1.8667
         Pairwise
     Significance

           0.1293
Time =    48.00 (Females)  for 067889

   alpha     =    0.050

   One tailed test (binomial) for chromosomal aberration  (pet. damaged cells)
     (with positive controls)
           Aberrant      Cells    Percent        SEM
   MG/KG      Cells     Scored   Aberrant  (for Obs)
    0.00          7        250     2.8000     1.3400
   12.50         13        250     5.2000     0.6110
         Pairwise
     Significance

           0.0855
                                     78

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex   Abs/Cell  Av Abs/Cell
   1      3     0.0  24.0    m      0.600        0.184
   2    133     0.0  24.0    m      0.800        0.200
                                     79

-------
filename:  C:\CA\CATEST02.ILS   date:              time:
    Endpoint:  Chromosomal aberration (total aberrations)

  Variance inflation factor:     1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           26.414    24   0.3325
  Sex              7.181    12   0.8454
  Time            17.934     8   0.0217
                                     80

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
24.00 (Males)   for 067889

        0.000
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         13
  500.00          8
 1000.00         15
 2000.00         37
               Cells Aberrat'ns
SEM
Pairwise
Scored
200
250
250
250
/ Cell (for Obs) Significance
0.0650
0.0320
0.0600
0.1480
0.0105
0.010
0.0149
0.0338

0.9463
0.5837
0.0043
Time =    48.00 (Males)  for 067889
   P
   alpha
        0.000
        0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         14
  500.00         20
 1000.00         37
 2000.00         60
               Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell (for Obs) Significance
0.0560
0.0800
0.1480
0.2400
0.0107
0.0239
0.0358
0.0404

0.1517
0.0006
0.0000
                                    81

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
72.00 (Males)   for 067889

        0.000
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         13
  500.00          9
 1000.00         22
 2000.00         35
               Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell
0.0520
0.0360
0.0880
0.1400
(for Obs)
0.0104
0.0093
0.0259
0.0322
Significance

0.8031
0.0641
0.0007
Time =    24.00 (Females)  for 067889
   P
   alpha
        0.001
        0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         10
  500.00          9
 1000.00         21
 2000.00         25
               Cells Aberrat'ns
SEM
Pairwise
Scored
250
250
250
250
/ Cell (for Obs) Significance
0.0400
0.0360
0.0840
0.1000
0.0060
0.0151
0.0334
0.0123

0.5907
0.0241
0.0056
                                     82

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
48.00 (Females)  for 067889

        0.000
   =    0.040  (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00          7
  500.00         17
 1000.00         27
 2000.00         34
               Cells Aberrat'ns
Scored
   250
   250
   250
   250
/ Cell
0.0280
0.0680
0.1080
0.1360
      SEM
(for Obs)
   0.0134
   0.0120
   0.0179
   0.0305
          Pairwise
      Significance

            0.0206
            0.0003
            0.0000
                                                            *
                                                            *
                                                            *
Time
   P
   alpha
72.00 (Females)  for 067889

        0.000
   -    0.040  (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         10
  500.00         11
 1000.00         19
 2000.00         35
               Cells Aberrat'ns
SEM
Scored
250
250
250
250
/ Cell
0.0400
0.0440
0.0760
0.1400
(for Obs)
0.0133
0.0093
0.0139
0.0225
                                   Pairwise
                               Significance

                                     0.4136
                                     0.0473
                                     0.0001
                                     83

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex   Abs/Cell  Av Abs/Cell
   1      3     0.0  24.0    m      0.600        0.184
   2    133     0.0  24.0    m      0.800        0.200
                                     84

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)

  Variance inflation factor:     1.000
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           26.414    24   0.3325
  Sex              7.181    12   0.8454
  Time             17.934     8   0.0217
                                    85

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time =    24.00 (Both sexes)  for 067889
   P
   alpha
        0.000
        0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         23
  500.00         17
 1000.00         36
 2000.00         62
               Cells Aberrat'ns
SEM
Pairwise
Scored
450
500
500
500
/ Cell
0.0511
0.0340
0.0720
0.1240
(for Obs)
0.0063
0.0088
0.0180
0.0183
Significance

0.9003
0.0985
0.0001
Time
   P
   alpha
48.00 (Both sexes)  for 067889

        0.000
   =    0.040 (scaled by .8, assuming next run to exclude high dose)
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
              Total
   MG/KG Aberrat'ns
    0.00         21
  500.00         37
 1000.00         64
 2000.00         94
               Cells Aberrat'ns
SEM
Pairwise
Scored
500
500
500
500
/ Cell
0.0420
0.0740
0.1280
0.1880
(for Obs)
0.0089
0.0131
0.0200
0.0274
Significance

0.0178
0.0000
0.0000
                                     86

-------
filename: C:\CA\CATEST02.ILS   date:              time:


Time =    72.00 (Both sexes)  for 067889

   p         =    0.000
   alpha     =    0.040 (scaled by .8,  assuming next run to exclude high dose)

   One tailed test (Poisson) for chromosomal aberration (total aberrations)
              Total      Cells Aberrat'ns        SEM       Pairwise
   MG/KG Aberrat'ns     Scored     / Cell  (for Obs)   Significance
    0.00         23        500     0.0460     0.0083
  500.00         20        500     0.0400     0.0065         0.6763
 1000.00         41        500     0.0820     0.0144         0.0122   *
 2000.00         70        500     0.1400     0.0191         0.0000   *
                                      87

-------
filename:  C:\CA\CATEST02.ILS   date:               time:
    Endpoint:  Chromosomal aberration (total aberrations)
  There are 2  outliers at the .05 significance level.

       Line    Dose  Time  Sex   Abs/Cell  Av Abs/Cell
   1      3     0.0  24.0    m      0.600        0.184
   2    133     0.0  24.0    m      0.800        0.200

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time =

   alpha
48.00 (Males)

        0.050
for 067889
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
     (with positive controls)
              Total
   MG/KG Aberrat'ns
    0.00         14
   12.50         76
               Cells Aberrat'ns
              Scored     / Cell
                 250     0.0560
                 250     0.3040
                        SEM
                  (for Obs)
                     0.0107
                     0.0560
    Pairwise
Significance

      0.0000
Time =

   alpha
48.00 (Females)

   =    0.050
  for 067889
   One tailed test (Poisson) for chromosomal aberration  (total aberrations)
     (with positive controls)
              Total
   MG/KG Aberrat'ns
    0.00          7
   12.50         58
               Cells Aberrat'ns
              Scored     / Cell
                 250     0.0280
                 250     0.2320
                        SEM
                  (for Obs)
                     0.0134
                     0.0259
    Pairwise
Significance

      0.0000
                                     89

-------
filename:  C:\CA\CATEST02.ILS   date:              time:
    Endpoint:  Mitotic Index

  Variance inflation factor:    1.341
  Alpha:                         0.050

  Factor         Deviance    df        p

  Scorer           52.058    24   0.0008
  Sex            206.145    24   0.0000
  Time           136.872    32   0.0000
                                     90

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
24.00 (Males)  for 067889

        0.768
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      196
      218
      213
      202
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
3.9200
4.3600
4.2600
4.0400
SEM
(for Obs)
0.5531
0.3874
0.2876
0.3745
                                   Pairwise
                               Significance

                                     0.8298
                                     0.7707
                                     0.6045
Time
   P
   alpha
48.00 (Males)  for 067889

        0.000
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      264
      179
      140
       64
 Cells
Scored
  5000
  5000
  5000
  5000
  Mitotic
Index (%)
   5.2800
   3.5800
   2.8000
   1.2800
      SEM
(for Obs)
   0.3492
   0.1849
   0.1789
   0.1937
    Pairwise
Significance

      0.0002
      0.0000
      0.0000
                                      91

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
72.00 (Males)   for 067889

   =    0.000
   =    0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      230
      206
      152
       76
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
4.6000
4.1200
3.0400
1.5200
SEM
(for Obs)
0.3876
0.3441
0.2596
0.2585
                                   Pairwise
                               Significance

                                     0.1551
                                     0.0002
                                     0.0000
Time =    24.00 (Females)  for 067889
   P
   alpha
        0.697
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      256
      241
      236
      227
 Cells
Scored
  5000
  5000
  5000
  5000
  Mitotic
Index (%)
   5.1200
   4.8200
   4.7200
   4.5400
      SEM
(for Obs)
   0.2313
   0.1093
   0.1794
   0.3724
    Pairwise
Significance

      0.2756
      0.2123
      0.1214
                                      92

-------
filename: C:\CA\CATEST02.ILS   date:
                                        time:
Time
   P
   alpha
48.00 (Females)  for 067889

        0.572
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      253
      239
      232
      219
Cells
Scored
5000
5000
5000
5000
Mitotic
Index (%)
5.0600
4.7800
4.6400
4.3800
SEM
(for Obs)
0.4927
0.3299
0.5504
0.4263
                                   Pairwise
                               Significance

                                     0.2881
                                     0.1993
                                     0.0831
Time
   P
   alpha
72.00 (Females)  for 067889

        0.707
        0.050
   Likelihood ratio for mitotic index
   MG/KG
    0.00
  500.00
 1000.00
 2000.00
 Cells in
  Mitosis
      222
      212
      240
      224
 Cells
Scored
  5000
  5000
  5000
  5000
  Mitotic
Index (%)
   4.4400
   4.2400
   4.8000
   4.4800
      SEM
(for Obs)
   0.4020
   0.3180
   0.1978
   0.3593
    Pairwise
Significance

      0.3359
      0.7705
      0.5333
                                       93

-------
filename: C:\CA\CATEST02.ILS   date:
                                       time:
Time =    48.00 (Males)  for 067889

   alpha     =    0.050

   Likelihood ratio for mitotic index   (with positive controls)
           Cells in      Cells    Mitotic        SEM
   MG/KG    Mitosis     Scored  Index (%)  (for Obs)
    0.00        264       5000     5.2800     0.3492
   12.50        243       5000     4.8600     0.4483
                                                Pairwise
                                            Significance

                                                  0.1692
Time =    48.00 (Females)  for 067889

   alpha     =    0.050

   Likelihood ratio for mitotic index
                             (with positive controls)
   MG/KG
    0.00
   12.50
Cells in
 Mitosis
     253
     253
 Cells
Scored
  5000
  5000
  Mitotic
Index (%)
   5.0600
   5.0600
      SEM
(for Obs)
   0.4927
   0.1492
    Pairwise
Significance

      0.5000
                                     94

-------