United Slates
                       Environmental Protection
                       Agency
v>EPA
                       Environmental Monitoring
                       Systems Laboratory
                       P.O. Box 93478
                       Las Vegas NV 89193-3478
EPA600/M-91/011
April 1991
The  Environmental
Monitoring  Systems
Laboratory  -  Las  Vegas
                       The Environmental Monitoring
                       Systems Laboratory - Las
                       Vegas (EMSL-LV) is one of
                       EPA's twelve national re-
                       search laboratories in its
                       Office of Research and
                       Development. Over 200 EPA
                       employees and 300 on-site
                       contractor personnel work at
                       the EMSL-LV, which has an
                       annual operating budget of
                       about $40 million. Its mission
                       is to develop, evaluate, and
                       apply methods and systems
                       for monitoring the environ-
                       ment.
                       The Laboratory was estab-
                       lished in 1955 as a U.S.
                       Public Health Service labora-
                       tory with responsibility for
                       monitoring radioactivity in
                       public areas around the
                       Nevada Test Site and other
                       nuclear explosive test sites.
                       Environmental radiation
                       monitoring and research
                       activities associated with the
                       U.S. Atomic Energy
                       Commission's nuclear testing
                       program were the sole pro-
                       grams conducted by the labo-
                       ratory through the 1960's.
                       This activity included a
                       radiation biology research
  program. When the Environ-
  mental Protection Agency was
  created in December 1970,
  the Laboratory became a part
  of the new Agency with an
  expanded mission to develop
  monitoring techniques for a
  variety of environmental pol-
  lutants and conduct environ-
  mental studies nationwide. In
  1972, the Environmental Pho-
  tographic Interpretation
  Center (EPIC) in Warrenton,
  Virginia, became a part of the
  Laboratory as an eastern
  facility for remote sensing
  support to EPA Regional and
  Program Offices.
The EMSL-L V Executive Center at night; part of a complex of buildings located on the campus of the University of Nevada-Las Vegas.

-------
Laboratory Programs
Advanced Analytical
Chemistry
A continuing theme for the
Laboratory has been re-
search on integrated expo-
sure of man to chemical and
radiological pollutants
through multiple environ-
mental pathways. Major in-
vestigative and technology-
developmental areas include:
  Major Program Areas
                                                      1)   Advanced Analytical Chemistry

                                                      2)   Field Monitoring

                                                      3)   Monitoring Network Design

                                                      4)   Field and Laboratory Quality Assurance

                                                      5)   Human Exposure Assessment

                                                      6)   Environmental Status and Trends
                                                     Unique Areas of Expertise
                                                     7)   Remote Sensing (Active and Passive)

                                                     8)   Subsurface Monitoring

                                                     9)   Geographic Information Systems

                                                     10)  Environmental Radiation Assessment

                                                     11)  Geostatistics
                                                     Special Projects
Measurement of an ever-in-
creasing number of organic
and inorganic contaminants
in complex environmental
matrices, at ever-increasing
levels of sensitivity, has re-
quired the development and
evaluation of innovative
techniques for sample
extraction and analysis.
Advanced techniques such
as liquid chromatography,
mass spectrometry, Fourier
transform infrared spectros-
copy, gas chromatography
and inductively-coupled
plasma spectroscopy are
developed and evaluated.
                                                   Advanced Analytical Methods Research

-------
 Field Monitoring
Monitoring Network
Design and
Geostatistics
 Laboratory evaluation and
 field validation of existing and
 emerging technologies for off-
 site measurement of toxicants
 at or around hazardous waste
 sites is the central activity of
 the advanced field monitoring
 methods program. This
 program addresses the need
 for rapid, low-cost field
 methods to support hazard-
 ous waste site monitoring and
 characterization activities.
 The costs of site characteri-
 zation are a direct result of
 sampling, analyses and
 associated quality assurance
 activities required to deter-
 mine the suitability of data for
 environmental decision
 making. Portable x-ray fluo-
 rescence spectrometer and
 gas chromatograph methods,
 and highly specific chemical
 sensors and immunochemical
 test kits are capable of
 yielding immediate or quick
turnaround data that can
result in major cost savings
and expedite the cleanup
process. These instruments
and methods will enhance
EPA's ability to manage risks
posed by hazardous waste
sites.
                                                           Field Portable Test Kit for Immunochemical Environmental Monitoring
Monitoring systems design
and monitoring statistics are
rapidly advancing fields be-
cause of readily available
personal computers and their
inexpensive computing
power. At the same time, the
high cost of collection and
analysis of environmental
samples places a premium
on efficient and effective
study design and data
interpretation. The monitoring
statistics program is develop-
ing data-analysis techniques
for more defensible decision
making, computerized spatial
simulation for sampling plan
design and evaluation,
kriging software for personal
computers, and multivariate
methods for  spatial pattern
recognition. Each of these
activities is aimed at provid-
ing practical  help for environ-
mental investigators.  For
example, spatial data analy-
sis can provide maps of sites
showing isopleths of probabil-
ity exceeding a selected
contaminant
concentration.
                                                                               60 82
                                                           Surface Estimated by Kriging, with Corresponding Contour Map

-------
Field and Laboratory
Quality Assurance
Human Exposure
Assessment
 The Laboratory serves as the
 Agency's center for analytical
 laboratory quality assurance.
 Properly validated test
 methods are developed, and
 guidelines are prepared to
 enhance the Agency's ability
 to obtain reliable sample
 analyses through commercial
 laboratories. Studies are
 conducted to evaluate the
 performance of these labora-
 tories and to determine the
 precision and accuracy of
 analytical protocols. In the
 1980's the Laboratory
 assumed national leadership
 for monitoring and quality
 assurance aspects of the
 Agency's hazardous waste
 and pesticides programs.
                                                          Field and Laboratory Quality Assurance.
Human exposure assess-
ment provides critical infor-
mation required to make risk
estimates for environmental
pollutants. Exposure assess-
ments are conducted by
using predictive methods
(modeling), direct measure-
ments (monitoring), or by the
use of reconstructive tech-
niques (biomarkers). Labora-
tory projects utilizing the
predictive methods include
the evaluation and validation
of indoor air models and the
development of a model to
estimate the exposure of
humans to benzene. Projects
utilizing the direct measure-
ment approach include the
measurement of benzene
concentrations in various
microenvironments and the
use of personal exposure
monitors (PEMs) to measure
the exposure of nitrogen
oxides to humans. Recon-
structive  approaches for ex-
posure assessments are
being evaluated for possible
inclusion into future monitor-
ing programs. These include
the use of DMA adducts,
protein adducts (hemoglobin
and serum albumen) carrier
proteins (e.g., metal-
lothionein), receptors, conju-
gation systems (e.g., glu-
tathione), porphyrin ratio
changes, and lesion-specific
endonculeases. In addition,
biotechnology monitoring
guidelines are being devel-
oped for the release of ge-
netically engineered microor-
ganisms (GEMs) in agricul-
tural experiments. Emphasis
is being placed on sample
collection procedures,
comparison of sample types,
determination of aerosolized
bacterial half-life rates and
field study designs to monitor
GEMs.
                                                                                                         ^^^^»jffl|^H
                                                          Developing Human Exposure Models for Use in Exposure
                                                          Assessment

-------
 Ecological Monitoring
Remote Sensing
(Active and Passive)
 The Laboratory is participat-
 ing in the Agency's Environ-
 mental Monitoring and As-
 sessment Program (EMAP),
 a national research program
 to prevent unwanted or
 irreversible damage to the
 nation's ecosystems. EPA
 must know the current status
 of the ecosystem, be able to
 determine trends in health or
 deterioration, and be in
 position to regulate environ-
 mental  pollutants in order to
 protect these systems. The
 national research will clas-
 sify, characterize, and moni-
 tor status and trends of
 important ecosystems and
 their subclasses. The moni-
 toring efforts specifically
 focus on conditions over
 periods of years to decades.

 The EMSL-LV,  using ad-
 vanced monitoring methods,
 is determining status and
 trends in terrestrial ecosys-
 tems, specifically forests,
 agroecosystems,  grasslands,
 and deserts, Also, the
 Laboratory has general
 EMAP responsibility for
 conducting initial ecosystem
 characterization, providing
 remote sensing support,
 providing guidance and
 support for field logistics and
 quality assurance, and for
 developing and implementing
 a distributed data base man-
 agement system.
                                                           Monitoring the Status of an Ecosystem
In the1970's, the application
of aerial photography and
scanner imagery technologies
for environmental assess-
ments became an important
Laboratory program. The
Laboratory's aerial photogra-
phy interpretation facilities in
Las Vegas and its branch in
Warrenton, Virginia, became
EPA's center for environ-
mental monitoring using
overhead imagery from
aircraft and satellites. Appli-
cations of this technology
have included the detection
of waste discharges into wa-
terways and harbors, the
location of waste disposal
sites on land, lake-water
quality management, wetland
delineation, erosion identifi-
cation and other types of
surface degradation, and
quantifying locations of envi-
ronmental impacts associ-
ated with land-use practices.
As the EPA's center for this
type of monitoring technol-
ogy, much of the Laboratory
work in this area involves col-
lecting and analyzing aerial
imagery to support environ-
mental regulation compliance
investigations by EPA's
Regional Offices.
                              High Resolution
                              Satellite Imagery
                              Applications:

                              Left, High Spatial
                              Resolution - Urban
                              Mapping.

                              Right, High Spectral
                              Resolution -
                              Vegetation Analysis
                                                    Spot Quad Map -10 Meter Resolution    Landstat TM - 30 Meter Resolution

-------
Remote Sensing
(continued)
Subsurface Monitoring
Aircraft-borne laser-based
remote sensing devices are
being developed and applied
for the monitoring of urban
and regional scale environ-
mental problems. The
primary laser-based tool for
urban or regional air quality
assessment is an airborne
aerosol lidar, which is used to
study pollutant layer struc-
tures and atmospheric mo-
tions influenced by complex
terrain and coastal environs.
Another lidar device under
development will allow for the
detailed, concurrent meas-
urement of ozone, sulphur
dioxide, and perhaps nitrogen
dioxide in the atmosphere. In
anticipation of increased
monitoring  requirements,  a
feasibility study has been
initiated to identify and
evaluate remote sensing
techniques for safely monitor-
ing toxic and  hazardous
pollutants from a distance.
The related technology of
airborne laser fluorosensing
is used to measure a number
of water quality indicators in
lake, river, and estuarine
waters. These include
chlorophyll a. concentration,
which is an indicator of phyto-
plankton density; dissolved
organic carbon (DOC), which
is an indicator of the overall
level of dissolved organic
matter; and the optical
attenuation coefficient, which
is closely related to water
clarity. Research is being
directed to detecting and
mapping algae blooms which
can create toxic water
conditions in lakes.
                                                           Computerized Interpretation of Airborne Laser-Based Data
                                                           Gathering
EMSL-LV is conducting
ground-water monitoring
methods research to test and
improve methods or proce-
dures for detecting contami-
nation of ground water. Sub-
surface monitoring methods
are also under development
for detecting pollutants in the
unsaturated zone above the
ground-water table, and for
collecting soil gases to detect
volatile subsurface pollut-
ants. Geophysical methods
such as ground-penetrating
radar and geochemical
detection methods are tested
and developed for mapping
near-surface contaminant
plumes. Both surface-based
and downhole methods are
examined for the more
difficult problem of mapping
deeply buried contaminant
plumes associated with
injection wells. Advanced
technologies such as
downhole pollutant detection
with light-activated optrodes
at the end of an optical fiber
eliminate the need for exten-
sive well-drilling to collect
water samples. Other re-
search is conducted to
develop leak detection
devices for monitoring
underground storage tanks
used for gasoline and other
chemicals.
                             Geophysical Sensing to Detect Substance Conic
                                        n/nants

-------
Geographic Information
Systems
Environmental Radiation
Assessment
 This computer-based tech-
 nology combines data and
 automated cartography to
 map data gathered from field
 surveys, remote sensing
 instruments, and other
 information such as census
 data on population distribu-
 tion. Using a GIS, the envi-
 ronmental analyst can display
 and overlay "maps" of these
 data layers on a video
 monitor screen and conduct
 extensive calculations and
 operate mathematical models
 of environmental conditions.
 These types of analyses are
 important to the EPA be-
 cause they represent a com-
 puter-based "tool box"
 available to identify and
 model pollutant threats
 to human populations
 and ecosystems.
 EMSL-LV is the lead Labora-
 tory for GIS research and
 development to ascertain how
 GIS technology will fit into the
 assessment and enforcement
 activities of the Agency.
 The GIS research
 mission is being ad-
 vanced through a
 series of pilot
 projects to
 demon-
strate the technology for ex-
amining hazardous waste dis-
posal sites, wetland areas, air
pollution and ground-water
contamination situations.
                                                                                                           WASTES

                                                                                                         ROADS

                                                                                                     HYDROLOGY

                                                                                                 SAMPLE SITES

                                                                                                 SOIL/WATER
                                                                                             CHEMISTRY SAMPLES

                                                                                          BUILDINGS
                                                                                       TOPOGRAPHY

                                                                                     DRAINAGE BASINS
                                                                           Hazardous Waste Site
                                                          Geographic Information Systems (GIS) Data Layers
The Laboratory's radiation
monitoring program provides
the framework for document-
ing radiation exposures of
populations living  near the
Nevada Test Site  (NTS) and
other nuclear test  sites.
Mobile monitoring teams are
deployed around the NTS
during nuclear test periods. If
radioactivity  is  released, these
teams are prepared to work
with local officials  in directing
protective actions, including
evacuation of residents, if
necessary. Air and ground-
water sampling networks
measure off-site radiation
levels on a continuing basis.
Programs  for sampling milk,
cattle, and wildlife  detect inad-
vertent contamination. Ther-
moluminescent dosimeters, in
place at about  130 fixed loca-
tions in addition to those worn
every day  by approximately
50 off-site  residents, measure
accumulated radiation expo-
sure levels. The Laboratory
also operates a whole-body
counter that measures levels
of natural and man-made
radionuclides in bone, tissue,
and internal organs of resi-
dents living around the NTS.
In cooperation with the U.S.
Department of Energy, the
Laboratory has established 18
Community Monitoring Stations
around the NTS and placed
them under the supervision of
local residents. The radiation
data, collected every five
minutes from solar-powered
gamma radiation detection in-
struments, is transmitted to
the Laboratory via satellite
relay. A visual readout at the
station allows local residents
to observe exposure level
measurements at any time.
                                                          Community Radiation Monitoring Station

-------
 Special Projects
The Laboratory has, over the
years, undertaken a number
of special projects utilizing its
broad monitoring capability.
Examples include the emer-
gency radiation monitoring
program for the Three Mile
Island nuclear power reactor
incident, the National Lake
Eutrophication Survey, Love
Canal contamination studies,
Missouri dioxin studies, and
the  National  Surface Water
Survey and Direct Delayed
Response Project as a part of
the  EPA responsibility under
the  Acid Precipitation Act of
1980.
Technical support, either in
the form of technology
transfer (training personnel in
other EPA offices or states on
how to use EMSL-LV technol-
ogy) or technical assistance
(helping others conduct
environmental studies), is
provided  in all of the program
areas described earlier. For
example, the  Laboratory was
assigned the responsibility for
designing the quality assur-
ance program for EPA's
research  project to evaluate
bioremediation enhancement
for the  (Valdez) Alaska oil
spill.

EMSL-LV, 944 East Harmon Avenue, Las Vegas NV 89119

-------