U.S. ENVIRONMENTAL PROTECTION AGENCY
           NATIONAL EUTROPHICATION SURVEY
                    WORKING PAPER SERIES
                                         REPORT
                                           ON
                                        SALINE LAKE
                                      LASALLE PARISH
                                         LOUISIANA
                                       EPA REGION VI
                                    WORKING PAPER No, 542
CORVALLIS ENVIRONMENTAL RESEARCH LABORATORY - CORVALLIS, OREGON
                            and
ENVIRONMENTAL MONITORING & SUPPORT LABORATORY - LAS VEGAS, NEVADA
 699-440

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                                   REPORT
                                     ON
                                 SALINE LAKE
                               LASALLE PARISH
                                  LOUISIANA
                                EPA REGION VI
                            WORKING PAPER No, 542
         WlTH THE COOPERATION OF THE
LOUISIANA WILD LIFE AND FISHERIES COWISSION
                   AND THE
          LOUISIANA NATIONAL GUARD
                 MARCH, 1977

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            REPORT ON SALINE LAKE

          LASALLE PARISH, LOUISIANA

                EPA REGION VI


                     by

       National  Eutrophication Survey

        Water and Land Quality Branch
       Monitoring Operations Division
Environmental Monitoring  & Support Laboratory
              Las Vegas,  Nevada

                     and

           Special Studies Branch
 Corvallis Environmental  Research Laboratory
              Corvallis,  Oregon
            Working Paper No.  542
     OFFICE OF RESEARCH AND DEVELOPMENT
    U.S.  ENVIRONMENTAL PROTECTION AGENCY

                 March 1977

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                          CONTENTS

                                                       Page
Foreword                                                i i
List of Louisiana Study Lakes                           iv
Lake and Drainage Area Map                               v
Sections
  I.  Introduction                                       1
 II.  Conclusions                                        1
III.  Lake Characteristics                               3
 IV.  Lake Water Quality Summary                         4
  V.  Literature Reviewed                                9
 VI.  Appendices                                        10

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                               FOREWORD
     The National Eutrophication Survey was Initiated in 1972  in
response to an Administration commitment to investigate the  nation-
wide threat of accelerated eutrophication to freshwater lakes  and
reservoirs.

OBJECTIVES

     The Survey was designed to develop, in conjunction with state
environmental agencies, information on nutrient sources, concen-
trations, and impact on selected freshwater lakes as a basis for
formulating comprehensive and coordinated national, regional,  and
state management practices relating to point source discharge
reduction and nonpoint source pollution abatement in lake water-
sheds.

ANALYTIC APPROACH

     The mathematical and statistical procedures selected for  the
Survey's eutrophication analysis are based on related concepts
that:

     a.   A generalized representation or model relating
          sources, concentrations, and impacts can be
          constructed.

     b.   By applying measurements of relevant parameters
          associated with lake degradation, the generalized
          model can be transformed into an operational
          representation of a lake, its drainage basin, and
          related nutrients.

     c.   With such a transformation, an assessment of the
          potential for eutrophication control can be made.

LAKE ANALYSIS

     In this report, the first stage of evaluation of lake and
watershed data collected from the study lake and its drainage
basin is documented.  The report is formatted to provide state
environmental agencies with specific information for basin
 Elanninq [§303(e)], water quality-criteria/standards review
 §303(c)], clean lakes [§314(a,b)], and water quality monitoring
[§106 and §305(b)] activities mandated by the Federal Water
Pollution Control Act Amendments of 1972.

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                                   m
     Beyond the single lake analysis, broader based correlations
between nutrient concentrations (and loading) and trophic condition
are being made to advance the rationale and data base for refine-
ment of nutrient water quality criteria for the Nation's freshwater
lakes.  Likewise, multivariate evaluations for the relationships
between land use, nutrient export, and trophic condition, by lake
class or use, are being developed to assist in the formulation of
planning guidelines and policies by the U.S. Environmental Protection
Agency and to augment plans implementation by the states.

ACKNOWLEDGMENTS

     The staff of the National Eutrophlcation Survey (Office of
Research and Development, U.S. Environmental Protection Agency)
expresses sincere appreciation to the Louisiana Wild Life and
Fisheries Commission, Division of Water Pollution Control for
professional involvement, to the Louisiana National Guard for
conducting the tributary sampling phase of the Survey, and to
those Louisiana wastewater treatment plant operators who pro-
vided effluent samples and flow data.

     Robert A. Lafleur, Chief; J. Dale Givens, Assistant Chief;
Lewis R. Still, Biologist; Louis Johnson, Biologist; Lee Cau-
barreaux, Biologist; Darrell Reed, Engineer; Dempsey Alford,
Biologist; and Elwood Goodwin, Hater Quality Control Technician,
all of the Louisiana Wild Life and Fisheries Commission, Division
of Water Pollution Control reviewed the preliminary reports and
provided critiques most useful in the preparation of this
Working Paper Series.

     Major General O'Neil Daigle, Jr., the Adjutant General of
Louisiana, and Project Officer Colonel Lawrence P. Dupre, who
directed the volunteer efforts of the Louisiana National Guards-
men, are also gratefully acknowledged for their assistance to
the Survey.

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                    NATIONAL EUTROPHICATION SURVEY
                              STUDY LAKES
                          STATE OF LOUISIANA
LAKE NAME
Anacoco Lake
Lake Bistineau
Black Bayou
Black Lake
Bruin Lake
Bundick Lake
Caddo Lake

Cocodrie Lake
Cocodrie Lake (Lower)
Concordia Lake
Cotile Lake
Cross Lake
D'Arbonne Lake
False River Lake
Indian Creek Reservoir
Saline Lake
Turkey Creek Lake
Lake Vernon
lake Verret
  PARISH
Vernon
Bienville, Webster
Caddo
Natchitoches and Red River
Tensas
Beauregard
Caddo (Menon and Harrison
  in Texas)
Concordia
Rapi des
Concordia
Rapides
Caddo
Union
Pointe Coupee
Rapides
LaSalle
Franklin
Vernon
Assumption

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 SALINE   LAKE
X  Lake Sampling Site

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                   REPORT ON SALINE LAKE,  LOUISIANA
                            STORE! NO.  2214

I.    INTRODUCTION
          Saline Lake was included in the  National  Eutrophication  Survey
     (NES) as a water body of interest to  the Louisiana Stream Control
     Commission and Louisiana Wild Life and Fisheries  Commission.   Tri-
     butaries and nutrient sources were not sampled, and this  report
     relates only to the data obtained from lake sampling.
II.   CONCLUSIONS
     A.    Trophic Condition:*
               Survey data indicate that Saline Lake is eutrophic,  i.e.,
          nutrient rich and highly productive.   Whether such nutrient
          enrichment is to be considered beneficial or deleterious  is
          determined by its actual or potential  impact upon designated
          beneficial  water uses.
               Potential  for primary production as  measured by algal assay
          control  yield was high.   Chlorophyll  a_ values ranged from 4.6
          yg/1  in the fall  to 34.9 yg/1  in the  summer, with a  mean  of 15.3
          yg/1.   Of the 19 Louisiana lakes sampled  in  1974, 3  had  higher
          median total  phosphorus, 4 had higher orthophosphorus, 1  had
          higher median inorganic  nitrogen and  none had lower  Secchi disc
          visibility than Saline Lake.

     *See  Appendix C.

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          Survey limnologists did not report any nuisance conditions
     in the lake.
B..   Rate-Limiting Nutrient:
          Autumn algal assay results, suggest that Saline Lake was
     growth limited by available phosphorus, levels.   Spikes with
     phosphorus, or nitrogen and phosphorus simultaneously resulted
     in increases in assay yield.  No growth, response accompanied
     the addition of nitrogen.  The lake inorganic nitrogen to ortho-
     phosphorus (N/P) ratios indicate phosphorus limitation in summer
     and fall, and nitrogen limitation in the spring.  However, pro-
     duction in the highly turbid waters was more likely limited by
     available Tight, at least during spring and fall sampling periods,
     than by nutrients.

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III.  LAKE CHARACTERISTICS

     A.    Lake Morphometry:*
                                     2
          A.    Surface area:  7.98 km .
          B.    Mean depth:   2.7 meters.
          C.    Maximum depth:   5.3 meters.
          D.    Volume:  21.546 x 106 m3.

     B.    Precipitation:

          A.    Year of sampling:  165.8 cm.
          B.    Mean annual:   151.6 cm.
     *Provided by the State of Louisiana.

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IV.   .LAKE WATER QUALITY SUMMARY
          .Saline Lake was sampled three times during the open-
     water season of 1974 by means of a pontoon-equipped Huey
     helicopter.  Each time, samples for physical and chemical
     parameters were collected from two stations on the lake and
     from one or more depths at each station (see map, page v).
     During each visit, depth-integrated samples were collected
     from each station for chlorophyll a_ analysis and phytoplankton
     identification and enumeration.  During the first and last
     visits, 18.9-liter depth-integrated samples were composited for
     algal assays.  Maximum depths sampled were 4.3 meters at Station
     01 and 3.0 meters at Station 02.  For a more detailed explana-
     tion of NES methods, see NES Working Paper No. 175.
          The results obtained are presented in full in Appendix C
     and are summarized in IV-A for waters at the surface and at the
     maximum depth for each site.   Results of the phytoplankton
     counts and chlorophyll a_ determinations are included in IV-B.
     Results of the limiting nutrient study are presented in IV-C.

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SALINE LAKE
STOKET CODE 2214
                    PHYSICAL  AND CHEMICAL  CHARACTERISTICS
(   3/19/74  )                         (   5/29/74 )
                                                 MAX
                                                                                                          (  11/12/74  )
                                                                                                                       MAX
PARAMETER
N.
RANGE
MEDIAN
OEPIH
(METERS) N»
S»»o
KANGE
= 2
MEDIAN
DEPTH
RANGE
(METERS) N»

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B.   Biological Characteristics:

     1..   Phytoplankton -
          Sampling
          Date

          03/19/74
          05/29/74
          11/12/74
     2.   Chlorophyll a_

          Sampling
          Date

          03/19/74
          05/29/74


          11/12/74
Dominant
Genera

1.  Flagellates
2.  Dactylococcopsis
3.  Melosira
4.  Crucigenia
5.  Dinobryon

     Other genera

          Total

1.  Flagellates
2.  Melosira
3.  Ankistrodesmus
4.  Kirchneriella
.5.  Nitzschia

     Other genera

          Total
1.
2.
3.
4.
5.
Melosira
Nitzschia
Cryptomonas
Flagellates
Trachelomonas
                               Other genera

                                    Total
    Station
    Number

      01
      02

      01
      02

      01
      02
 Algal
 Units
 per ml

  1,244
    474
    237
    237
    118

    531

  2,841

    909
    795
    625
    454
    454

  1,423

  4,660

    287
    172
    115
     57
     57
                            688
Chlorophyll a^
    7.4
   10.1

   34.9
   29.7

    4.6
    5.3

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Ortho P
Cone. (mg/1)
0.035
0.085
0.085
0.035
Inorganic N
Cone. (mg/1)
0.739
0.739
1.739
1.739
Maximum yield
(mq/l-dry wt. )
5.2
17.6
26.6
6.0
C.   Limiting Nutrient Study:
     1.   Autoclaved, filtered, and nutrient spiked - 11/12/74

          Spike(mg/l)
          Control
          0.05 P
          0.05 P + 1.0 N
          1.00 N
     2.   Discussion -
               The control yield of the assay alga, Selenastrum capri-
          cornutum, indicates that the potential for primary production
          in Saline Lake was high at the time of autumn assay sample col-
          lection.  The increase in yield with the addition of phosphorus
          as well as the lack of significant response to the addition of
          nitrogen indicates phosphorus limitation.  Maximum yield was
          achieved with the simultaneous addition of both nutrients.
               It should be noted that significant chemical changes
          took place in Louisiana lake samples between collection and
          assay analysis.   The assay data should be considered in this
          context and until such differences are resolved, used with
          caution for any predictions of actual  lake conditions.  Such
          chemical changes are likely to alter the control yield as well
          as modifying the N/P ratio.  The spring algal  assay results have
          not been included as these substantial changes in nutrient levels
          have removed their information value.

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     The N/P ratios in the summer and fall  lake chemistry



samples were respectively 17/1 and 19/1, indicating phos-



phorus limitation at those times.  The spring N/P ratio



was 8/1, suggesting nitrogen limitation (a  mean N/P ratio



of 14/T or greater generally reflects phosphorus limitation)

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LITERATURE REVIEWED

U.S.  Environmental  Protection Agency.   1975.   National  Eutrophica-
  tion Survey Methods 1973-1976.   Working Paper No.  175.   National
  Environmental  Research Center,  Las Vegas, Nevada,  and Pacific
  Northwest Environmental Research Laboratory, Corvallis,  Oregon.

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                                10





VI.  APPENDICES
                            APPENDIX A



                        CONVERSION FACTORS

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                CONVERSION FACTORS




Hectares x 2.471 = acres



Kilometers x 0.6214 = miles



Meters x 3.281 = feet

                         -4
Cubic meters x 8.107 x 10   = acre/feet


Square kilometers x 0.3861 = square miles



Cubic meters/sec x 35.315 = cubic feet/sec



Centimeters x 0.3937.= inches



Kilograms x 2.205 = pounds



Kilograms/square kilometer x 5.711 = Ibs/square mile

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        APPENDIX B



PHYSICAL AND CHEMICAL DATA

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STORET SET91EVAL DATE  7b/12/ll
NATL EUTROPrtlCATION
EPArLAS VEGAS
           221401
          31  51 02.0 092 56 *7.0
          SALINE LAKE
          22       LOUISIANA

OATE
F«QM
TO
74/03/19


74/05/29


74/11/12



TIwE UEpTri
OF
DAY FEET
15 05 0000
15 05 0005
15 05 0014
15 30 0000
is 30 boos
15 30 OOQ8
09 10 ooon
09 10 OOQ5
09 10 OOOH
00010
WATEW
TEMH
CENT
19.6
19.6
19.4
28.6
28.3
28.2
15. H
15.8
15.8
                                    5.6
                                    6.0
                                    6,0
                                    8.6
                                    8.6
                                    8.8
                                 00300     00077     00044
                                  DO      ThANSP   CNDUCTVt
                                          SECChl   FIELD
                                 MG/L     INCHES   MICKOMHO
                                               10
 74
 78
 76
 99
101
log
143
142
142
lltPALES
4
0'04QO
PH

su
6.50
6.35
6.40
7.20
7.10
7.10
b.74
6.75
6.79

00410
T AL*.
CAC03
MG/L
14
13
12
16
16
15
10K
28
10K
211
0019
00610
NH3-N
TOTAL
MG/L
0.120
0.120
0.110
0.190
0.210
0.220
0.070
0.070
0.060
12<^


FEET OEPTH
UOt^b
TOf XJEL
M
MG/L
0.700
0.600
0.800
1.000
0.800
0.900
1 .000
0.400
0.800
00630
N02f.N03
N-TOTAL
MG/L
0.200
0.190
0.170
0.160
0.190
0.200
O.f.30
0.650
0.680
00671
PMOS-ois
OHTHO
MG/L P
0.056
0.029
0.028
0.020
0.026
0.023
0.051
0.020
0.087
DATE
FROM
TO
74/03/19


74/05/29


74/U/12


00665 32217 00031
TIME DEPTH PHOS-TOT CH.LRPHYL INCDT LT
OF A HEMNING
DAY FEET
15
15
15
15
15
15
09
09
09
05
05
05
30
30
30
10
10
10
0000
0005
0014
0000
0005
OOOB
0000
0005
0008
MG/L P MG./L PEHCENT
0.108 7.4
0,107
0.100
O.ll2 34,9 50,0
0.111
0.134
0,233 4,6
0.247
0,251
       K VALUE KNOWN TO BE LESS THAN    	
   —    '       INDICATED

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STOHET RETRIEVAL Dfl»TE 75/12^11
NATL EUTROPHICATION SUHVEY
EPA-LAS VEGAS
 32U02
31 51 02.0 092 56 47."
SALINE LAKt
2?      LOUISIANA
DATE
FHQM
TO
74/03/19


74/05/29

74/11/12


DATE
FROM
TO
74/03/19


74/05/29

74/11/12

TI^E
OF
DAY
15
15
15
15
15
09
09



30
30
30
45
45
30
30

TIME
OF
DAY
15
15
15
15
15
09
09


30
30
30
45
45
30
30
DEPTH

FEET
0000
0005
0010
0000
0006
0000
0005

DEPTH

FEET
0000
ooos
0010
0000
0006
0000
0005
00010
WATER
TEMP
CENT

19.7
19.6
27.2
27.2
15.5
15.5
00665
PHOS-TOT

MG/L P
0.080
0.081
0.081
0.111
0.105
0.245
0.233
00300 U0077 00094
DO THANSP CNDUCTVY

MG/L

5
5

5
9
9
3221
SECCHl FIELD
INCHES MICPOMHO

.6 72
.6 71
5 77
.4 7B
.0 8 119
.0 115
7 00031
11EPALE.S 2111202
4 0015 FEET DEt-'TH
00400 00410 00610 UOfV!-> 00630
PM T ALK NH3-N TOT KJtL N02J>N03
CAC03 TOTAL
SU MG/L MG/L
6.1b 11 0.100
6.10 13 0.110
6.10 13 0.100
6.90 14 0.170
6.85 13 0.190
6.68 28 0.070
6.70 29 0.0*0

N
MG/L
0.600
0.700
O.bOO
1.200
0.900
1.000
1.000

00671
OHOS-OIS
N-TOTAL O^THO
MG/L
0
0
0
0
0
0
0

.130
.140
.150
.160
.160
.620
.620

MIJ/L P
0.022
0.030
0.036
0.019
0.019
0.024
O.Ol-i

CHLHPHYL INCDT LT
A
UG/L
10


29

5

RFMNING
PERCENT
.1


.7 50.0

.3















































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         APPENDIX C

PARAMETRIC RANKINGS OF LAKES
   SAMPLED BY NES IN 1974


     STATE OF LOUISIANA

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LAKE DATA TO BE USED IN RANKINGS
LAKE
CODE  LAKE NAME
2201  ANACOCO LAKE
2202  BRUIN LAKE
2?03  LAKE 6ISTINEAU
2204  BLACK 8AYOU
2206  BnNOICK LAKE
2207  COCODRIE LAKE
2208  COTILE LAKE
2209  CONCORDIA LAKE
2210  CROSS LAKE
2211  D'ARBONNE LAKE
2212  FALSE RIVER LAKE
2213  INDIAN CREEK
2214  SALINE LAKE
2215  TURKEY CREEK LAKE
2216  LAKE VERRET
2217  LAKE VERNON
?219  BLACK LAKE
2220  COCO'DWIE
4807  CADDO LAKE
nEniAN
TOTAL P
0.031
O.n57
O.n61
0.0*6
0.157
0.090
0.037
0.076
0.057
O.n38
0.082
o.nai
0.111
0.1 76
0.163
O.Olfl
0.077
0. 106
0.049
MEDIAN
INORG N
0.0*0
0.250
0.100
0.090
0.13S
0.400
0.100
0.080
0.080
0.100
0.130
O.lbO
0.350
0.170
0.100
0.120
0.150
0.050
0.070
500-
MEAN SEC
455.833
450.333
4S3.000
453.417
469.667
479.000
442.333
46B.333
475.250
458.250
442.500
458.333
493.000
477.833
481.428
436.667
454.000
478.333
463.562
MEAN
CHLOWA
8. 700
16.350
12.^33
17.818
20.467
35.300
12.650
32.950
38.385
6.300
24.550
21.467
15.333
21.967
62.02b
4.900
12.733
33.433
20.12b
15-
MIN oo
10.400
15.000
13.200
12.200
10.600
7.700
14.000
14.800
11.400
13.200
14.900
14.800
9.600
14.600
12.000
14.400
11.600
11. BOO
10.000
HEOUN
OISS ORTHO P
0.007
0.012
0.01H
0.009
0.073
0.026
0.011
'0.009
0.010
0.011
0.023
0.010
0.025
0.033
0.056
0.007
0.015
0.014
0.00ft

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PERCENT OF LAKES WITH HIGHER VALUES  (NLiMRFN OF LAKES WITH HIGHER  VALUES)
LAKE
CODE  LAKE NAME
2301  AMACOCO LAKE
2202  BRUIN LAKE
2203  LAKE BISTINEAU
22^4  BLACK BAYOU
2205  BUNDICK LAKE
2207  COCODRIE LAKE
2208  COTILE LAKE
2209  CONCOROIA LAKE
2210  CROSS LAKE
2211  D'ARBONNE LAKE
221*  FALSE RIVER  LAKE
2213  INDIAN CREEK
2214  SALINE LAKE
2215  TURKEY CREEK  LAKE
2216  LAKE VERRET
2217  LAKE VERNON
2219  BLACK LAKE
222ri  COCODRIE
4607  CADOO LAKE
MEDIAN
TOTAL »-
32
M
KO
72
11
21
63
44
56
78
3^
92
17
0
6
ion
39
22
67
( 16)
( 11)
( 9)
( 13)
( 2)
( 5)
( 15)
( 8)
( 10)
( 14)
( 6)
( 16)
( 3)
( 0)
( 1)
( 18)
( 7)
( 4)
( 12)
MEDIAN
INORG N
83 (
11 (
58 (
72 (
33 (
0 (
58 (
83 (
83 (
58 (
39 (
28 (
6 (
17 (
58 (
44 <
22 (
100 (
94 (
14)
2)
9)
13)
6)
0)
9)
14)
14)
9)
7)
5)
1)
3)
9)
e>
4)
18)
17)
500-
MEAiy SEC
t>7
83
tol
79
33
11
94
39
28
56
89
50
0
22
6
100
72
17
44
( 12)
( 15)
( 11)
( 14)
( 6)
( 2)
( 17)
( 7)
( 5)
( 10)
( 16)
( 9)
( 0)
( 4)
( 1)
( 18)
( 13)
( 3)
( 8)
MEAN
CHLOKA
89
61
72
56
44
11
83
22
6
94
28
39
67
33
0
100
78
17
50
( 16)
( 11)
( 13)
( 10)
( 8)
( 2)
( 15>
( 4)
( 1)
( 17)
( 5)
( 7)
( 12)
( 6)
( 0)
( 18)
( 14)
( 3)
( 9)
15-
MIN DO
83 (
0 (
42 (
50 (
78 (
100 (
33 (
14 (
72 (
42 (
6 (
14 (
94 (
22 (
56 (
28 (
67 (
61 (
89 (
15)
0)
7)
9)
14)
13)
6)
2)
13)
7)
1)
2)
17)
4)
10)
5)
12)
11)
16)
MEDIAN
oiss ORTHO
94 (
50 (
33 (
bl (
0 (
17 (
61 (
81 (
69 (
56 (
28 (
69 (
22 (
11 (
6 (
100 (
39 (
44 (
H9 (
17)
9)
6)
14)
0)
3)
11)
14)
1?)
10)
5)
1?)
4)
2)
1)
18)
7)
fl)
16)

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