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United States Environmental Protection Agency ~
Region III
Office of Analytical Services & Quality Assurance ^5%^l/
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Sample Submission Procedures
for the
Office of Analytical Services
& Quality Assurance
Laboratory Branch
Revision 12.0
January 2009
Environmental Science Center
701 Mapes Road
Fort Meade. Maryland 20755-5350
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903B09001
OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 2 of27
Sample Submission Procedures for OASQA
Laboratory Branch
Issued by the Office of Analytical Services & Quality Assurance
(OASQA)
Effective Date: l/ktilSLOol
Revision Date: \I$L% /SLOOl
Number: Revision 12.0
Author
Name: Patricia Sosinski
Signature: Ytfa&M /Ofaiwifa
Date:
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Reviewer
Name: Jill^Bilyeu
Signature:]
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Date:
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Approval •.-,'•:; : :
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Name: Cynthia E. Caporale
Signature: ^X^j^. t£*
Title: Laboratory Branch
Chief
Date: /-Zf'Oj
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OASQA Sample Submission Procedures Rev. 12.0
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TABLE OF CONTENTS
1.0 Introduction 4
2.0 Project Planning and Analytical Request Preparation and Scheduling 4
3.0 Sampling 4
3.1 Collection Requirements 4
3.2 Holding Times 5
3.3 Sample Types 5
3.4 Blanks 5
3.5 Laboratory Quality Control Requirements 6
4.0 Paperwork Requirements , 7
5.0 Shipping Requirements 8
6.0 Shipment Notification 8
7.0 Pollution Prevention and EMS 9
8.0 References 10
TABLE NOTES 11
TABLE I 12
'TABLE n is
TABLE III 22
Attachment 1 - Example Chain-of-Custody 23
Attachment 2 - Example Sample Tag 24
Attachment 3 - Example Hazard and Risk Exposure Data Sheet 25
Attachment 4 - OASQA Sample Acceptance Policy 26
Attachment 5 - Region III 5035A Fact Sheet 27
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1.0 Introduction
This document specifies the procedures that are to be followed when submitting samples to
the Office of Analytical Services and Quality Assurance (OASQA) Laboratory Branch located
at Ft. Meade, Maryland. These procedures will help ensure that the field and laboratory
aspects of the sampling event are linked in a way to produce reliable data of known quality.
2.0 Project Planning and Analytical Request Preparation and Scheduling
An approved Quality Assurance Project Plan (QAPP) should be in place before project
scheduling. For information on QAPPs, please contact a member of the OASQA Technical
Services Branch, Quality Assistance Project Plan specialists listed below.
Quality Assistance Project Plan contacts:
Robin Danesi: 410-305-2607 (ph), 410-305-3095 (fax) danesi.robin@epa.gov
Mary Ellen Schultz:410-305-2746(ph).410-305-3095ffax) schultz.maryellen@epa.gov
Mike Mahoney: 410-305-2631 (ph), 410-305-3095 (fax) mahoney.mike@epa.qov
Ed Messer: 410-305-2744 (ph), 410-305-3095 (fax) messer.ed@epa.gov
All analytical requests are scheduled through the OASQA Technical Services Branch. See
the EPA Region III, Users'Guide for Acquiring Analytical Services for instructions on
preparing and submitting Analytical Requests, and other documentation requirements.
(Current version found at http://www.eDa.qov/region03/esc/labservices.htm)
Analytical Request contacts:
John Kwedar: 410-305-3021 (ph), 410-305-3095 (fax) kwedar.john@epa.gov
Dan Slizys: 410-305-2734 (ph), 410-305-3095 (fax) slizvs.dan@epa.qov
Carroll Harris: 410-305-2625 (ph), 410-305-3095 (fax) harris.carroll@epa.gov
3.0 Sampling
Proper collection and identification of samples, documentation of the collection event (in
permanent field records), and submittal of required paperwork (i.e., Chain of Custody,
Hazardous Exposure sheets) are all essential parts of a successful sampling event. When
samples are not properly collected, preserved, or shipped, the quality of the data may be
compromised. If this occurs, the requestor will be notified and given the opportunity to
resample or to receive qualified data. Refer to the OASQA Sample Acceptance Policy
(Attachment 4). In some cases it may be possible for the laboratory to complete the
analysis with some alteration. For example, if there is insufficient sample volume,, the data
may have to be reported with increased quantitation limits or the requestor may be asked
to prioritize analyses.
3.1 Collection Requirements
Tables I, II, III and IV show preservatives, minimum volumes/weights, container types and
holding times for aqueous and non-aqueous samples submitted to OASQA.
IMPORTANT: AH differences due to the requirements of the drinking water
program (SDWA) are listed in Table III. In addition, the TABLE NOTES for Tables I, II
and III summarize the critical information needed for sample collection. It is essential to
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comply with these requirements so that reliable data that meet the needs of the project are
produced.
3.2 Holding Times
Samplers must be aware of the holding times for all analyses requested and ship
samples to OASQA as quickly as possible. Holding times are calculated from the time
and date of sample collection and not the date of receipt at the lab. To ensure that OASQA
can meet the required holding time, it may be necessary to ship samples at the end of each
collection day.
PLEASE NOTE: OASQA does not accept samples on weekends or holidays at this time.
Please plan your sample collection accordingly so that samples are collected, shipped and
analyzed within holding times.
Data from samples analyzed past the stated holding time must be carefully examined by
the data user. These values may be biased low due to possible loss of the parameter(s) of
interest, and they will be flagged by the laboratory as such.
3.3 Sample Types
Sample collection types normally used are defined as follows:
Grab sample - An individual sample collected over a period of time generally not exceeding 15
mfnutes. A grab sample is normally associated with water or wastewater sampling. However, soil,
solid, oil, sediment, and liquid hazardous waste samples, for example, may also be considered grab
samples.
Composite sample - A sample containing discrete allquots (1) collected over a defined time period at
equal time intervals (time composite), (2) collected In volumes proportional to the flow rate (flow
proportional composite), or (3) composited from individual grab samples collected on an area or
cross-sectional basis (area composite).
3.4 Blanks
Blank samples are used to identify potential sources of contamination during sampling,
shipping, storage and analysis. Blanks should be specified as part of every QAPP or
sampling plan. It is highly recommended that field blanks accompany all sample sets, with
the exception of VOAs In Air.
All water used for blanks must be lab pure water, demonstrated to be free of the
parameter(s) of interest. The water may have to be tested prior to use. Commercially
available HPLC water is not acceptable for most blank uses. HPLC water is not certified as
"organic-free" and/or "metal-free", and therefore, may contain compounds of interest.
Blanks that are preserved must be prepared with the same stock and same volume of the
preservative that was used with the samples.
The blank types normally used are defined as follows:
Sample Matrix ("Field") Blank: The field blank is used to determine whether contamination has been
introduced during sample collection, storage and shipment, as well sample handling in the analytical
laboratory. Field blanks are prepared by transferring demonstrated analyte-free water to the
appropriate sample containers during the time when site-specific samples are collected. These
blanks are transported to the field and exposed to the same conditions as site-specific samples
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including removal of the container caps and addition of any appropriate preservatives. Field blanks
should be collected whenever aqueous samples are collected and at a frequency of one per 20
samples. This sample should be analyzed for the same parameters as those associated with site-
specific samples collected from potentially contaminated media.
Trip blank: The trip blank Is used to determine whether contamination has been introduced to
aqueous samples through cross-contamination during shipment and storage of sample containers,
for volatile organic compounds (VOCs) only. Trip blanks should be prepared prior to the sampling .
event, including preservatives, and are not exposed to field conditions. They may be furnished by
the analytical laboratory and will consist of demonstrated analyte-free water provided in the
appropriate container (i.e., 40 ml teflon-lined glass vial). Trip blanks should be collected at a
frequency of one per each cooler used to store/transport site-specific samples designated for
aqueous VOC analyses (or one for each day that VOCs are collected).
Rinsate or sampling equipment blank: The rinsate blank is used to determine whether the sampling
equipment decontamination procedure has been adequately performed, thereby assuring that no
"carryover" contamination has been introduced before (or during) sample collection. Rinsate blanks
are prepared in the field by pouring demonstrated analyte-free water through/over the sampling
equipment (including filters) and collecting rinsate in the appropriate sample containers and adding
appropriate preservatives. Rinsate blanks should be collected at a frequency of one per 20 samples
per matrix per sampling equipment type (or one per day per matrix per equipment type). This
sample should be analyzed for the same parameters as those associated with site-specific samples
collected from potentially contaminated media.
Temperature blank'. The temperature blank is used only to determine whether site-specific samples
have been adequately cooled during shipment and storage. Temperature blanks can be prepared
any time before or during field sampling activities by adding water to an appropriate sample
container such as a VGA vial. Temperature blanks should be collected at a frequency of one per
each cooler used to store/transport site-specific samples. The temperature of this sample should be
measured upon receipt by the analytical laboratory but not analyzed.
3.5 Laboratory Quality Control Requirements
In addition to the minimum volumes or weights needed to perform a single analysis for
each parameter listed in Tables I, II and III, it is essential that the sampler collect enough
sample to allow the laboratory to analyze samples for quality control (QC) purposes. For
each parameter, the laboratory will need 3 times the minimum volume for one
sample per batch of:
10 samples for inorganic parameters
20 samples for organic parameters
This volume will be used for the quality control analysis for each batch of
samples. If there are more than 10 (inorganic) or 20 (organic) samples per batch, another
sample with extra volume should be collected for each group. For example, in a set of 11
to 20 samples for inorganic parameters, there should be extra volume collected for two
samples.
It is extremely important that sufficient volume be collected for quality control analysis.
This is especially true for organic analysis, because of the large volume requirements. A
liter of sample will be needed for EACH parameter or analytical fraction, and a sufficient
volume for quality control analysis (3 times sample volume) must be collected for each
parameter or fraction. For example, for each sample collected for organic analysis for
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SVGAs and Pest/PCB with QC, you will need to collect 3 liters for SVGAs and 3 liters for
Pest/PCB, for a total of 6 liters. ON THE OTHER HAND, please do not collect any more
volume than necessary; it is expensive to properly dispose of excess volume and is
inefficient for both the sampler and laboratory. (See Section 7.0 Pollution Prevention and
EMS.)
4.0 Paperwork Requirements
Region III requires the use of the FORMS II LITE for preparation of the sampling
documentation, with certain exceptions (see below). FORMS II LITE is an electronic
windows-based application which automates sampling event documentation. It generates a
Chain of Custody Form (COC), sample tags, and container labels. FORMS II LITE software is
available for downloading at: http://dyncsdaol.fedcsc.com/itg/forms2lite/.
When access to FORMS II LITE is not practical, hard copy versions of the paperwork may
be used. Instructions for accessing the paper versions of the COC, sample tags, and
custody seals are available by contacting the Technical Services Branch Analytical Request
Specialists. See Attachments 1 and 2 for examples of completed forms. The following
documents must accompany the sample shipment for accurate identification and safety
information.
Sample Tag - Each sample must have a sample tag tied to the container and some type of
adhesive label with identifying information. Each sample tag and label must be legibly
written with indelible (i.e., waterproof) ink. (See Attachment 2 for an example.) The
information that is written on the sample label must match the information on the Chain of
Custody form (COC). The sample tag must be tied on each container so that it will not fall
or slip off. Please do not use tape to secure labels or tags since it will loosen and fall off if
the containers get cold or wet. No erasures or white outs are allowed. All errors must be
corrected with a single line through the error, initialed and dated. For the safety of lab
staff, indicate on each sample label and sample tag any preservative used for the samples.
Exposure Data Sheet - Each time samples are collected, the sampler must complete a
Hazard and Risk Exposure Data Sheet (Attachment 3). This information helps ensure the
safety of the lab staff receiving the samples so that proper precautions are taken whenever
potentially hazardous samples are encountered. This sheet is a vital part of the OASQA
safety program and must be attached to the OUTSIDE of at least one shipping container
so that it is available for review by the sample managers before opening any coolers or
chests.
Chain of Custody (COC) - A COC must accompany each sample shipment. (See
Attachment 1 for an example.) A sample shipment without a COC may be rejected by the
laboratory. The COC must be sealed in a water proof zip-locked bag and taped on
the inside of the ice chest lid with the
samples. Always use indelible ink (never pencil) for all markings on the COC. The original
record must accompany the shipment and a copy retained by the sampler. Each distinct
sample must appear on a separate line. It is NOT necessary to have a separate line for
each container (or each sample tag). NOTE: Samples collected for dissolved constituents
are considered distinct from the unfiltered aliquot and should be placed on a separate line.
Any writing errors made on the COC must be crossed out with a single line, initialed, dated
and rewritten.
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Chain of Custody documentation must include:
site name (project name)
sampler's name/signature
sample ID (station number)
date and time of collection (recorded in 24 hour clock time)
type of sample (grab or composite)
sample description (station location) (indicate if sample has been filtered for
dissolved components or if it is a field duplicate)
number of containers
parameters requested (i.e., tests, methods)
sample tag numbers (in remarks)
date, time and signatures for sample receipt and transfer
If problems are found with the documentation or the physical condition of the samples
upon arrival at the laboratory, the Sample Scheduling Coordinator may require a Letter to
File from the sampler to document problems and corrective actions taken.
5.0 Shipping Requirements
The OASQA laboratory will not accept any samples shipped with any particulate
(dusty) type packing material/ especially vermiculite. We recommend using plastic
bags to double-bag glass containers to prevent leakage, then wrapping each container in
bubble wrap to prevent breakage. To further assure that any leakage'will be contained, the
cooler should be lined with.a large plastic bag. Bags of ice (sealed to contain the melted
water) should be used for cooling the samples. To ensure uniform cooling, the bags must
be packed on top and around the samples themselves and not merely placed on the sides
of the packed chest. The loaded cooler must not be heavier than 50 pounds to allow
for safe handling.
SAMPLES PRESERVED BY CHILLING MUST BE SHIPPED WITH SUFFICIENT ICE TO REMAIN
AT < 6°C WHILE IN TRANSIT. A temperature blank (VOA vial filled with water) must be
included in the shipment to allow the laboratory to verify the temperature upon receipt.
The chest must be sealed with strapping tape and custody seals on the outside. The
custody seal must be placed so that it will be broken when the chest is opened. Department
of Transportation (DOT) and/or Federal Express approved shipping containers must be
used. If the samples have been identified as 'environmental laboratory samples'such as
those defined in Appendix D of the Sample Shipping Procedures reference (see Section
8.1), then the shipment process is not regulated. In most cases, preserved water,
wastewater, and sediment samples accepted by OASQA for analysis are considered
environmental samples. If the samples have been classified as 'hazardous materials', only
certified personnel are allowed to ship the containers, according to the Dangerous
Goods Regulations promulgated by the International Air Transport Authority (IATA) (see
Section 8.2). At least one member of the sampling team should be aware of the DOT and
IATA legal requirements for shipping these types of materials,
6.0 Shipment Notification
Always notify the Sample Scheduling Coordinator (SSC) when samples have been shipped.
In addition, notify the SSC as soon as possible if a scheduled shipment has been canceled
or there are any changes in the number or types of samples. These changes may affect the
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laboratory's scheduled workload. All notifications or changes should be phoned,
faxed, or e-mailed on or BEFORE the expected shipping date. Sample shipments
cannot be accepted before or after normal business hours (7:30am - 4:30pm), on
weekends or Federal Holidays.
Sample Scheduling Coordinator (SSC) Contacts:
Patricia Sosinski: 410-305-2667 (ph), 410-305-3093 (fax), sosinski.pat@epa.gov
Kevin Martin: 410-305-3032 (ph), 410-305-3040 (fax), martin.kevin@epa.QOV
Mailing Address:
P. Sosinski/Kevin Martin
U.S. EPA, Region III, OASQA
Environmental Science Center
701 Mapes Road
Fort Meade, Maryland 20755-5350
NOTE: If any part of the Sample Submission Guidelines is unclear or if you want to verify
the requirements for collection and shipping, please call one of the contacts listed above.
Many times problems can be avoided if issues are clarified before the samples are collected
and sent. In addition, if there is a need for parameters not listed in this document, the
laboratory may be able to accommodate special requests. Please call the Technical Services
Branch contacts listed in Section 2.0 for information.
7.0 Pollution Prevention and EMS
It is the Environmental Science Center's policy to integrate environmental stewardship into
our operations and we have therefore instituted an Environmental Management System
(EMS). This means that we manage our organizations and our programs in a manner that
protects the environment, the safety of our employees, and the public health. In support
of this policy, the OASQA lab is committed to the promotion of pollution prevention (P2)
awareness and the Agency's waste reduction strategies. To support our P2 goals, the lab
requests that samplers take a common sense approach to the collection of samples with
respect to how much volume is collected. Of course, the most important consideration must
be the need for enough volume to constitute a representative sample, and to accommodate
the analysis requested. But remember many parameters can be combined
together to avoid excess volumes. Since the laboratory must pay to dispose of the
material after analyses, if at all possible, please combine samples for all analytes requiring
the same container and preservative in a minimum number of containers.
Examples of parameters for aqueous samples which are commonly combined are: (1)
metals + mercury + hardness; or (2) ammonia + TOC + TP + TKN. Parameters for solid
samples have few preservative requirements; therefore, most inorganic or organic
parameters can be combined in one container. It is especially important to consolidate
parameters when collecting solid samples because of the difficulty in disposing of the
excess sample. Solids should be collected in a single 8 oz. container for.either the organic
or inorganic parameters. For additional guidance on combining samples, please contact the
Sample Scheduling Coordinator (SSC).
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8.0 References
8.1 Letter from U.S. Department of Transportation (DOT) stating DOT's position on
shipping preserved environmental samples dated February 13, 2003.
(http://www.epa.gov/epawaste/hazard/testmethods/pdfs/pres-samp.pdf).
8.2 Dangerous Goods Regulations, International Air Transport Authority (IATA). Current
Edition (document changes annually).
8.3 EPA Order 1000.18, February 16, 1979,
(http://epawww.epa.gov/rmpolicy/ads/transorders.htm
8.4 Contract Laboratory Program (CLP) Guidance for Field Samplers, OSWER 9240.0-35,
EPA540-R-07-06, fhttp://www.epa.QOv/superfund/proarams/clp/Quidance.htm#sample).
8.5 EPA Region III, Users' Guide for Acquiring Analytical Services, current version
http://www.epa.gov/reQionQ3/esc/5users guide rev.pdf
8.6 Sample Submission Procedures for the Office of Analytical Services & Quality
Assurance Laboratory Branch, current version
http://www.epa.aov/reaion03/esc/labservices.htm
8.7 National Environmental Laboratory Accreditation Conference (NELAC) Standards,
current revision, http://www.nelac-institute.org/standards.php
8.8 "40 CFR Parts 122, 136, 141, 143, 430, 455, and 465; Guidelines Establishing Test
Procedures for the Analysis of Pollutants Under the Clean Water Act; National Primary
Drinking Water Regulations; and National Secondary Drinking Water Regulations; Analysis
and Sampling Procedures, Final Rule" Federal Register Vol. 72, No. 47 (Monday, March 12,
2007)11200 - 11249.
http://www.epa.gov/EPA-WATER/20Q7/March/Dav-12/wl073.pdf
8.9 "40 CFR Parts 136 and 503; Guidelines Establishing Test Procedures for the Analysis of
Pollutants; Analytical methods for Biological Pollutants in Wastewater and Sewage Sludge;
Final Rule" Federal Register Vol. 72, No. 57 (Monday, March 26, 2007) 14220 - 14233.
http://www.epa.QOV/fedrgstr/EPA-WATER/2007/March/Day-26/wl455.pdf
8.10 Electronic Code of Federal Regulations e-CFR; Approved analytical methods for
NPDES 40 CFR part 136.
http: //ecfr. g poaccess. go v/cg i/t/text/text-
Jdx?c=ecfr&tDl=/ecfrbrowse/TirJe40/40cfrl36 main O2.tpl
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TABLE NOTES
The following information applies to all parameters listed in the Sample Requirements
Tables I, II, and III. Preservations, holding times, and container types are taken from the specific
methods used for analysis or from Federal Register promulgated requirements.
1 BLANKS
All parameters except VOAs in Air require the collection of at least one blank sample as described in
Section 3.4
* Field Blank — collected for every parameter to ensure that containers and preservatives (if
required) are contamination free.
* Trip Blank — collected for aqueous volatile organics (VOCs) only. It must be prepared prior to the
sampling trip. NOTE: A Trip Blank is not required for VOCs in Air.
* Rinsate or Equipment Blank — collected if samples were filtered in the field or otherwise treated by
mechanical or physical means to check for carry-over contamination.
* Temperature Blank — placed in cooler whenever samples are required to be kept on ice. This Is
used for temperature verification only; it is not analyzed.
2 LABORATORY QUALITY CONTROL REQUIREMENTS
The sampler must collect 3 times the minimum volume for at least one sample per batch
of 10 inorganic samples or 20 organic samples for each parameter or analytical fraction—
to use for quality control (QC) analysis. It is extremely important that sufficient volume be
collected. (See Section 3.5 for further explanation.)
3 POLLUTION PREVENTION AND HAZARDOUS WASTE REDUCTION
If the sampling requirements (for preservation, sample containers, etc.) are exactly the same, then
one sample can be taken for several parameters in a single container if the volume will
accommodate all analyses (see Section 7.0 for further explanation). It is especially important to
consolidate parameters when collecting solid samples because of the difficulty in disposing of the
excess sa'mple. Solids should be collected in a single 8 oz. container for either the organic or
inorganic parameters. If a parameter cannot be combined with other parameters, it is noted in the
table.
4 COMPOSITING SAMPLES
Normally, samples will not be C9mposited in the laboratory. If it is appropriate or advantageous,
compositing in the lab will be considered on a case-by-case basis. Samples for the determination of
volatiles are not to be composited in the field because of possible loss of analyte.
5 DISSOLVED ANALYSIS
Samples collected for analysis of dissolved components must be filtered in the field. A filtered Field
Blank must also be collected for each new lot of filters. These are considered separate samples from
the unfiltered aliquot, so a separate sample number will be needed on the chain-of-custody form.
6 DECHLORINATION
Dechlorlnate only those samples which actually contain chlorine. Chlorine presence may be
determined using a color wheel or Hach kit.
If any of part of this document is unclear or if you want to verify the requirements, please
call the Sample Scheduling Coordinator (SSC) for clarification. It is highly recommended
that samples be transported to the laboratory as soon as possible after collection.
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TABLE I
AqueOUS Sample Requirements 1
Parameter
Acidity
Alcohols
Alkalinity
Anions by 1C:
Bromide
Chloride
Fluoride
Nitrite
Nitrate
Ortho Phosphate
Sulfate
Ammonia
Biochemical Oxygen
Demand (BOD)
Bromide
Carbonaceous
Biochemical Oxygen
Demand (CBOD)
Chemical Oxygen
Demand (COD)
Chloride
Chlorophyll &
Pheophytin
Coliform (total &
fecal)
Sample
Type4'5
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Grab
Container
Type
Glass or Plastic
Glass VOA
40-ml vial with
Teflon lined cap
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Wrap in
aluminum foil
and plastic bag.
Protect from
sunlight!
Glass or Plastic,
Sterile container
Minimum
Volume 2'3
500ml
10ml
500ml
200ml
200ml
500ml
200 ml
500 ml
200ml
200ml
See method
120 ml with
1-inch air
space in
bottle
Preservation
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, .<6 °C,
sulfuric acid to pH<2
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, ;<6 °C,
sulfuric acid to pH<2
none
See method for field
filtration/extraction
procedure.
cool, j<6 °C,
0,2ml/120ml of sterile
10 % sodium
thiosulfate6
Holding
Time (from
time of
collection)
14 days
7 days
14 days
48 hrs for
Nitrate,
Nitrite and
Ortho
Phosphate
28 days - all
others
28 days
48 hours
28 days
48 hours
28 days
28 days
Filters: store
at -20°C for
up to 21
days
6 hours
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TABLE I
AqueOUS Sample Requirements 1
Parameter
Color
Corrosivity (pH by
9040)
Total Cyanide
1,4 - DJoxane
Diesel Range
Organics (DROs)
Dissolved Organic
Carbon (DOC)
Fluoride
Hardness
Heterotrophic Plate
Count (HPC)
Sample
Type 4'5
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Container
Type
Glass or Plastic
Plastic
Glass or Plastic
Glass VOA 40-
ml vial w/Teflon
lined cap
Amber glass
bottle w/Teflon
lined cap
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic,
Sterile container
Minimum
Volume 2'3
200 ml
200 ml
200 ml
120 ml
(3 vials filled
with no
headspace)
1000 ml
(Cannot
combine
parameters)
100ml
200ml
200 ml
120 ml with
1-inch air
space in
bottle
Preservation
cool, <6 °C
none
Superfund: Test for
sulfide & oxidizing
agents & treat as per
ILM05.4, 8.1.1. Add
sodium hydroxide to
pH>12, coo!4°C
NPDES: In the
absence of
interferences, add
sodium hydroxide to
pH>12, cool^6°C
If interferences are
present, follow
footnotes from Table
II, 3/12/07 FR
cool, <6 °C
acidify with 1:1 HCI to
pH<2; , cool to <6
°C
filter in field, sulfuric
acid to pH<2, cool <6
°C
none
nitric acid to pH<2
cool, <10°C,
0.2ml/120ml of sterile
10 % sodium
thiosulfate6
Holding
Time (from
time of
collection)
48 hours
analyze as
soon as
possible
Superfund:
14 days
NPDES: 48
hours if
su If ides are
present,
otherwise 14
days.
14 days
14 days
28 days
28 days
6 months
8 hours
-------
OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 14 of 27
TABLE I
AqueOUS Sample Requirements 1
Parameter
Hexavalent
Chromium
HPLC/MS screen
Ignitability .
Infrared (IR) screen
Mercury
Metals
Nitroaromatics +
Nitramines,
Explosives
Nitrogen, Nitrate
Nitrogen, Nitrite
Nitrogen, Nitrate &
Nitrite combined
Nitrogen, Total
Kjeldahl (TKN)
Sample
Type 4'5
Grab
Grab or
Composite
Grab or
Composite
Grab or
composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Container
Type
Glass or Plastic
Amber glass
bottle w/Teflon
lined cap
Glass VGA 40-
ml vial
Glass or Plastic
Glass preferred
Plastic ok
Glass or Plastic
Amber glass
bottle w/Teflon
lined cap
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Minimum
Volume 2'3
200 ml
1000 ml
(Cannot
combine
parameters)
240 ml (six
40 ml vials)
100 ml
200ml
200ml
1000 ml
(Cannot
combine
parameters)
200ml
200 ml
200 ml
200 ml
Preservation
Field filter, add
ammonium sulfate
buffer to pH= 9.3 -
9.7, cool ^6 °C
See Footnote 20,
Table II, 3/12/07 FR
cool, j<6 °C
none
cool, <.6 °C - no
preservative
nitric acid to pH<2
(acid may be added at
the lab if safety
precautions warrant)
nitric acid to pH<2
(acid may be added at
the lab if safety
precautions warrant)
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
sulfuricacid to pH<2
cool, <.6 °C,
sulfuric acid to pH<2
Holding
Time (from
time of
collection)
28 days
7 days
analyze as
soon as
possible
analyze as
soon as
possible
28 days
6 months
7 days
48 hours
48 hours
28 days
28 days
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 15 of 27
TABLE I
AqueOUS Sample Requirements 1
Parameter
Total Nitrogen (TN)
Nitroglycerin
Oil & Grease
Perchlorate
Polyaromatic
Hydrocarbons
(PAHs) by GC/MS
PCB Congeners
PCB/Pesticides
Phenol, Total
Sample
Type 4'5
Grab or
Composite
Grab or
Composite
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Container
Type
Glass or Plastic
Amber glass
bottle with
Teflon lined cap
Glass wide-
mouth jar
(contact lab for
jar details)
Glass or Plastic
Amber glass
bottle w/Teflon
lined cap
Amber glass
bottle w/Teflon
lined cap
Amber glass
bottle w/Teflon
lined cap
Glass
Minimum
Volume a/3
250 mL
1000 ml
(Cannot
combine
parameters)
two - 1000
ml
containers
(Cannot
combine
parameters)
200 mL
one - 1000
ml
containers
(Cannot
combine
parameters)
two - 1000
mL
containers
(cannot
combine
parameters)
one - 1000
ml
containers
(Cannot
combine
parameters)
1000 ml
Preservation
cool, <.6 °C, sulfuric
acid to pH<2
cool, <6 °C
cool, <6 °C, sulfuric
or hydrochloric acid to
pH<2
none, but must avoid
temperature extremes
cool, <6 °C
cool, <6 °C
cool, <.6 °C
Dechlorinate6 with
excess ferrous
ammonium sulfate,
sulfuric acid to <6 °C,
coolj 4°C
Holding
Time (from
time of
collection)
28 days
7 days
28 days
28 days
7 days
1 year
7 days
NPDES
Pesticides -
if not pH 5-
9, Extract
within 72
hours
28 days
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 16 of 27
TABLE I
AqueOUS Sample Requirements *
Parameter
Phosphorus, Ortho
Phosphorus, Total
Semivolatiles
(SVGAs)
Solids, Total (TS)
Solids, Total
Dissolved (TDS)
Solids, Total
Suspended (TSS)
Sulfate
Sulfide
TCLP - Semivolatiles
(SVGAs)
TCLP - Herbicides
TCLP - Metals
TCLP - Mercury .
Sample
Type 4'5
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Container
Type
Glass or Plastic
Glass or Plastic
Amber glass
bottle w/Teflon
lined cap
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Glass or Plastic
Amber glass
bottle w/Teflon
lined cap
Amber glass
bottle w/Teflon
lined cap
Glass or Plastic
Glass or Plastic
Minimum
Volume 2'3
200 ml
200 ml
one - 1000
ml
containers
(Cannot
combine
parameters)
500 ml
500 ml
500 ml
200 ml
600 ml (2 -
300 ml BOD
bottles)
At least
1000 ml
At least
1000 ml
At least
1000 ml
At least
1000 ml
Preservation
cool, <6 °C, must be
field filtered for
NPDES compliance
cool, <.6 °C,
sulfuricacid to.pH<2
cool, ;<6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C, zinc
acetate &. sodium
hydroxide to pH>9
r
cool, ^6 °C (unless
cooling causes
precipitation of the
waste)
cool, <6 °C (unless
cooling causes
precipitation of the
waste)
cool, <6 °C (unless
cooling causes
precipitation of the
waste)
cool, <6 °C (unless
cooling causes
precipitation of the
waste)
Holding
Time (from
time of
collection)
48 hours
28 days
7 days
7 days
7 days
7 days
28 days
7 days
14 days
14 days
180 days
28 days.
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 17 of27
TABLE I
AqueOUS Sample Requirements 1
Parameter
TCLP - Pesticides
TCLP/ZHE -
Volatiles (VOAs)
Total Toxic Organics
(TTO)
Customer to supply
compound lists
Total Organic
Carbon (TOO
Volatiles (VOAs)
Sample
Type 4'5
Grab or
Composite
Grab
Follow
require-
ments for
PCB/Pests,
SVOAs, and
VOAs.
Grab or
Composite
Grab
Container
Type
Amber glass
bottle w/Teflon
lined cap
Glass VOA 40 -
ml vial w/Teflon
lined cap
Follow
requirements
for PCB/Pests,
SVOAs, and
VOAs.
Glass or Plastic
Glass VOA 40-
ml via! w/Teflon
lined cap
Minimum
Volume 2-3
At least
1000 ml
160 ml (4
vials filled
with no
headspace)
Follow
requirement
sfor
PCB/Pests,
SVOAs, and
VOAs.
100 ml
120 ml
(3 vials filled
with no
headspace)
Preservation
cool, <:6 °C (unless
cooling causes
precipitation of the
waste)
cool, <6 °C
Follow requirements
for PCB/Pests, SVOAs,
and VOAs.
cool, <6 °C,
sulfuric acid to pH<2
cool, <6 °C
Dechlorinate6 with
sodium thiosulfate
(10mg/40ml) BEFORE
acidifying with 1:1
hydrochloric acid to
pH<2. ForRCRAand
NPDES, samples must
be submitted both
preserved and
unpreserved If 2-
chloroethyl vinyl ether
is an analyte of
interest. This is due
to losses of 2-
chloroethyl vinyl ether
In acidified samples.
Holding
Time (from
time of
collection)
14 days
14 days
Same as for
PCB/Pests,
SVOAs, and
VOAs.
28 days
7 days
unpreserved
14 days
preserved
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 18 of 27
TABLE II
NOn-AqUeOUS Sample Requirements x
Parameter
Anions by 1C:
Bromide
Chloride
Fluoride
Nitrite
Nitrate
Ortho Phosphate
Sulfate
Benthic
Invertebrate
Taxonomy
Chemical Oxygen
Demand (COD)
Cyanide, Total
Diesel Range
Organics (DROs)
[
Dry Weight, Percent
Grain Size
Hexavalent
Chromium
HPLC/MS screen
Infrared (IR)
Screen
Mercury
Metals
Sampling
Type4
Grab or
Composite
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Container
Type
Glass (4 oz.
wide-mouth)
Glass
Glass (4 oz.
wide-mouth)
Glass (4 oz.
wide-mouth)
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Glass (4 oz.
wide-mouth)
Heavy plastic bag
or glass jar
Glass (4 oz.
wide- mouth)
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Glass (4 oz.
wide-mouth)
Glass (4 oz.
wide-mouth)
Glass (4 oz.
wide-mouth)
Appropriate
Weight2'3
50 g
N/A
50 g
50 g
50 g
50 g
500 g
50 g
50 g
50 g
50 g
50 g
Preservation
cool, <6 °C
70% ethanol
cool, .<6 °C
cool, <6 °C
cool, <6 °C
none
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
Holding
Time (from
time of
collection)
none
none
28 days
28 days
14 days
none
none
30 days
14 days
see
parameter of
interest
28 days
6 months
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 19 of 27
TABLE II
Non-AqueOUS Sample Requirements 1
Parameter
Metals in Wipes
Metals in Air Filters
Metals in Dust
Nitroaromatics +
Nitramines,
Explosives
Nitrogen, Total
Kjeldahl (TKN)
Nitroglycerin
Paint Filter Test
Perchlorate
Polyaromatlc
Hydrocarbons
(PAHs) by GC/MS
PCB Congeners
PCB/Pesticides
PCBs in oil
Sampling
Type4
Grab
Grab
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Container
Type
Plastic digestion
tube provided by
OASQA
Sealed plastic
bag
Vacuum bag
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Glass (4 oz.
wide-mouth)
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Glass or plastic
Glass (4 oz.
wide-mouth)
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Amber Glass (4
oz. wide- mouth
w/Teflon lined
lid)
Glass VOA 40-ml
vial w/Teflon
lined cap
Appropriate
Weight a'3
NA
NA
NA
50 g
50 g
50 g
500 g
50 g
50 g
50 g
50 g
10 g
Preservation
None
Include 5 unused
wipes for lab QC
None
Include 5 unused
filters for lab QC
None
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <.6 °C
cool, <6 °C
cool, .<6 °C
cool, <6 °C
cool, £6 °C
cool, .<6 °C
Holding
Time (from
time of
collection)
6 months
6 months
6 months
14 days
28 days
14 days
none
none
14 days
1 year
14 days
none
-------
OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 20 of 27-
TABLE II
Non-AqueOUS Sample Requirements 1
Parameter
pH by 9045C
Phenol, Total
Phosphorus, Total
Semivolatiles
(SVOAs)
TCLP-
Semivolatiles
(SVOAs)
TCLP - Herbicides
TCLP - Mercury
TCLP - Metals
TCLP - Pesticides
TCLP/ZHE -
Volatiles (VOAs)
Total Organic
Carbon (TOC)
Volatiles (VOAs)
Sampling
Type4
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab or
Composite
Grab
Grab
Grab
Container
Type
Glass (4 oz.
wide-mouth)
Glass (4 oz.
wide-mouth)
Glass (4 oz.
wide-mouth)
Amber Glass (4
oz. wide-mouth
w/Teflon lined
lid)
Amber Glass (8
oz. wide- mouth
w/Teflon lined
lid)
Amber Glass (8
oz. wide- mouth
w/Teflon lined
lid)
Glass (8 oz.
wide-mouth)
Glass (8 oz.
wide-mouth)
Amber Glass (8
oz. wide- mouth
w/Teflon lined
lid)
Glass VOA 40-ml
vial w/Teflon
lined cap
Glass (8 oz.
wide-mouth)
See attachment 5
Appropriate
Weight 2'3
50 g
50 g
50 g
50 g
200 g
200 g
200 g
200 g
200 g
160 g (4 .
vials filled
with no
headspace)
50 g
See
Attachment 5
Preservation
none
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, <6 °C
cool, .<6 °C
cool, <6 °C
cool, ^6 °C
cool, <.6 °C
cool, <6 °C
cool, .<6 °C
See Attachment 5
Holding
Time (from
time of
collection)
Analyze as
soon as
possible
28 days
28 days
14 days
14 days
14 days
28 days
180 days
14 days
14 days
none
48 hrs to
preserve or
analyze
-------
OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 21 of27
TABLE II
Non-AqiieOUS Sample Requirements 1
Parameter
Volatile Organic
Compounds (VOCs)
in air (JO14a/15)
Sampling
Type4
Grab or
Composite
Container
Type
Summa canister
supplied by lab
Appropriate
Weight 2'3
6L
Preservation
none
Holding
Time (from
time of
collection)
30 days
-------
OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 22 of 27
TABLE III
S D W A P rog ra IT! Special Requirements l> 6
Parameter
Coliform (total
& fecal)
Heterotrophic
Plate Count
(HPC)
Nitrate (NO3-
N)
Pesticides
Total
Trihalomethan
es (TTHMS)
Volatiles
(VOAs)
.
Sampling
Type 4'5
Grab
Grab
Grab or
Composite
Grab or
Composite
Grab
Grab
Container
Type
Glass or Plastic,
Sterile
Glass or Plastic,
Sterile container
Glass or Plastic
Amber glass
bottle w/Teflon
lined cap
Glass VGA 40-
ml vial w/Teflon
lined cap
Glass VOA 40-
ml vial w/Teflon
lined cap
Minimum
Volume 2'3
120 ml with 1-
inch air space in
bottle
120 ml with
1-inch air space
in bottle
200 ml
two - 1000 ml
containers
(Cannot
combine
parameters)
120 ml
(3 vials filled
with no
headspace)
120ml
(3 vials filled
with no
headspace)
Preservation
cool, <10°C, 0.2ml/120m[
of sterile 10 % sodium .
thiosulfate6
cool, <10°C, 0.2 ml/120ml
of sterile 10% sodium
thiosulfate6
From a chlorinated source
and cooled to <6 °C
cool, ^6 °C, 6 N HCI to pH
< 2, dechlorinate6 w/SO
mg sodium sulfite
cool, <6 °C, dechlorinate6
with 3 mg/40ml sodium
thiosulfate
cool, ;<6 °C, dechlorinate6
with either 25mg/40ml
ascorbic acid OR
3mg/40ml sodium
thiosulfate BEFORE
acidifying with 1:1
hydrochloric acid to pH<2.
Note: If residual chlorine
is measured in the field at
>5mg/L, to each vial add
an additional 25 mg of
ascorbic acid OR 3 mg of
sodium thiosulfate per
each increment of 5 mg/L
residual chlorine. Avoid
adding excess
dechlorination agent.
Holding
Time (from
time of
collection)
30 hours
'8 hours
14 days
7 days
14 days
14 days
preserved
-------
c
o
•
1
Relinquished by: (Signttvte)
Date /Time
Date /Time
Date /Time
Received by: ISiv>ituni
Received by: ISivatt/nl
Remarks
-------
OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 24 of 27
Attachment 2 - Example Sample Tag
o
s
s
i
**
*
l
Preservative:
Yes 'til No Q
ANALYSES
800 Anlons
6ollds eras)
COO, TQC, Nutrients
Phenollcs
Marewry
Cyanide
Dil and Grease
Orgaiiics GC/MS
Pffofity Pollutarrts
VoJalile Orgaitics
Pesticides
Mutagenictty
Bacteriology
Rcmaite:
Tag NO.
L«b Bwapto No.
3065951
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 25 of 27
Attachment 3 - Example Hazard and Risk Exposure Data Sheet
Region III, Office of Analytical Services and Quality Assurance
Ft Meade, Maryland
HAZARD AND RISK-EXPOSURE DATA SHEET
LEVELS OF PERSONAL PROTECTION DURING SAMPLING
BACKGROUND
Under the authority Section 104 of the Comprehensive Environmental Response,
Compensation, and Liability Act (CERCLA or Superfund) of 1980, Section 311 of the Clean
Water Act, and Subtitle I of the Resource Conservation and Recovery Act (RCRA), EPA has
been delegated the responsibility to undertake response actions with respect to the release or
potential release of oil, petroleum, or hazardous substances that pose a substantial threat to
human health or welfare, or the environment.
GENERAL
This form is to be used when collecting Environmental Samples (i.e. streams, farm ponds,
wells, soils etc.) and for Hazardous^ample (I.e. drums, storage tanks, lagoons, leachates,
hazardous waste sites). This information is intended for use as a guide for the safe handling
of these laboratory samples in accordance with EPA and OSHA regulations. The sample
classification(s) and levels of personal protection used by the sampler in all situations will
enable the analyst to be better aware of potential exposure to substances in air, splashes of
liquids, or other direct contact with material due to work being done.
DEGREE OF PROTECTION
Level A: Highest level of respiratory, skin, and eye protection needed. Fully
encapsulated suit, respirator self-contained (Tank type).
Level B: Highest level of respiratory protection but lesser level of skin protection needed.
Chemical suit, respirator self-contained (Tank type).
Level C: Lesser level of respiratory protection than Level B. Skin protection criteria are
similar to Level B. Chemical suit, canister respirator/cartridge
Level D: Work uniform without any respirator or skin hazards. Lab coat, gloves etc.
CLASSIFIED FIELD SAMPLES
Environmental Hazardous Comb. (Env. & Haz.) Radioactive
Site Name: Sampling Date:
Sta No. , . . , , ,
Field pH: , , , , ,
(must be taken prior to submission of aqueous samples)
Sampler: Work Phone Number:
Personal observations at time of sampling (surroundings):
Sample collection observations (physical sample, odors etc.):
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 26 of 27
Attachment 4 - OASQA Sample Acceptance Policy
OASQA Sample Acceptance Policy
The following are those conditions which indicate that the integrity of the sample may have
been jeopardized, either during the actual sampling event or during its shipment to the lab.
If one or more of these conditions exist, the laboratory will consult with the requester to
determine whether to reject the sample(s) for analysis or to perform a qualified analysis. If
the sample(s) is (are) rejected, the sampler will be given the opportunity to resample. If
the analysis is performed, the data will be reported with qualifiers explaining why the data
may have been compromised, and the potential impact on the data. In some cases it may
be possible for the laboratory to complete the analysis with some alteration. For example, if
there is insufficient sample volume, the data may have to be reported with increased
quantitation limits or the requestor may be asked to prioritize analytical requests.
The condition of sample(s) and shipment will be documented on the OASQA Shipment
Documentation Form. The Sample Scheduling Coordinator may request a Letter-To-File
from the sampler to document additional critical details. Any actions taken because of the
compromised condition of a sample willbe noted in the laboratory's information
management database and in the report narrative sent the requester.
Conditions which may jeopardize the integrity of the sample:
• Not collected in appropriate containers.
• If cooling is required for the requested analytes, samples are received at greater
than 6 degrees C or missing the temperature blank.
• Not properly preserved as outlined in Tables I, II, and/or III of the OASQA Sample
Submission Procedures.
• Received past the analytical holding time.
• Samples tampered with during shipment. (Example: custody seal has been broken)
• Insufficient sample to perform sample analysis or the quality control analysis.
• Sample identification incorrect, incomplete, or missing.
• Chain-of-custody documentation not available, inaccurate or incomplete.
• Samples inappropriate for requested analysis. (Example: decomposed condition)
• Leaking or broken container.
• Lack of a trip blank with samples collected for volatile analysis.
Note: One other condition which would cause samples to be rejected by OASQA is if the
samples are suspected to contain dioxin. At this time, this facility is not prepared to handle
the potential hazard of dioxin contamination.
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OASQA Sample Submission Procedures Rev. 12.0
January 2009
Page 27 of 27
Attachment 5 - Region III 5035A Fact Sheet
Region III 5035A Fact Sheet
May 15, 2003, Revision No: 2
Field Samplers Guide to the Collection and Handling of Soil Samples for
Volatile Organic Analysis using SW 846 Method 5035A
Summary:
The purpose of this fact sheet is to specify procedures for the collection and handling by
field samplers of soil samples for volatile organic analysis (VGA) in Region III. SW-846
Method 5035A is the collection method required for analysis of soil samples for VOA. This
method incorporates chemical preservatives and sample storage techniques to limit
volatilization and biodegradation of organic compounds. Method 5035A is applicable to both
low/medium and high level soil samples.
Collection Procedures:
• Soil samples being analyzed for volatile organic compounds collected via Method 5035A
should not be chemically preserved in the field.
• Samples should be collected using the following collection options:
Option 1: For most Soil types
Number of samples: 4 EnCore (or similar closed-sampling vessel)1 samplers
4 QC EnCore samplers
1 40 ml vial for moisture analysis
EnCore samplers (or similar sample collection device, refer to Section 4.5 of Method 5035)
Samples must be cooled to 4°C upon collection and during shipment and bagged
individually upon collection.
Samples must arrive at the laboratory within 24 hours.
Samples must be analyzed or preserved by the lab within 48 hours of collection.
Option 2: For Non-Cohesive Granular Material (wet, rocky, sediments, etc.)
Number of samples: 4 40mL vials (sampler may use wide mouth jars if sample
not amenable to smaller vials)
2 QC 40 ml vials
1 40 ml vial for moisture analysis
Samples must be cooled to 4°C upon collection and during shipment.
Samples must arrive at the laboratory within 24 hours.
Samples must be analyzed or preserved by the lab within 48 hours of collection.
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