SWRHL-54r

  ANIMAL INVESTIGATION PROGRAM BASELINE STUDIES:
              SUPPLEMENTAL TEST SITE "C"
                             by
Bruce P.  Hull, Jr., D. V. M. ,  and Stanley L.  Cohen, D. V. M.

               Animal Investigation Program
                 Bioenvironmental Research
         Southwestern Radiological Health Laboratory

        Department of Health, Education, and Welfare
                    Public Health Service
            National Center for Radiological Health
                     September 13, 1968
     This surveillance performed under a Memorandum of
                Understanding (No. SF 54  373)
                            for the
            U. S. ATOMIC ENERGY  COMMISSION

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                         LEGAL NOTICE


This report -was prepared as an account of  Government sponsored
•work.  Neither the United States, nor the Atomic Energy Commission,
nor any person acting on behalf of the Commission:

A.  makes any warranty or representation,  expressed or implied,
with respect to the accuracy, completeness,  or usefulness of the in-
formation contained in this  report, or that  the use of any information,
apparatus, method, or process disclosed in this report may not in-
fringe privately owned rights;  or

B.  assumes any liabilities with respect to the use of,  or for damages
resulting from the use of any information,  apparatus,  method, or pro-
cess disclosed in  this report.

As used  in the above,  "person acting on behalf of the Commission"
includes  any employee or contractor of the  Commission, or  employee
of such contractor,  to the extent that such employee or contractor of
the Commission,  or employee  of such contractor prepares,  dissemin-
ates, or  provides  access to, any information pursuant to his employ-
ment or  contract with the Commission, or his employment with such
contractor.
  077

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                                                        SWRHL-54r
 ANIMAL  INVESTIGATION  PROGRAM  BASELINE STUDIES:
            SUPPLEMENTAL TEST  SITE  "C"
                        by
  Bruce P. Hull, Jr., D.V.M., and Stanley L. Cohen, D.V.M.

           Animal  Investigation Program
             Bioenvironmental Research
   Southwestern Radiological Health Laboratory

  Department of Health, Education, and Welfare
              Public Health Service
     National Center for Radiological Health
                September 13, 1968
This surveillance performed under a Memorandum of
          Understanding (No. SF 54 373)
                     for the
         U. S. ATOMIC ENERGY COMMISSION

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                              ABSTRACT

The Animal Investigation Progam (AIP) collected tissue samples from
two Mule deer and three beef cattle for radioanalysis and histo-
pathologic studies prior to the Faultless event.  Pathologic lesions
present and detectable body burdens of selected radioisotopes were
comparable to those found in routine sacrifices.

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                           TABLE OF CONTENTS

ABSTRACT                                                             i
TABLE OF CONTENTS                                                    ii
LIST OF TABLES AND FIGURE                                           iii
INTRODUCTION                                                         1
PROCEDURES                                                           2
RESULTS AND DISCUSSION--RADIOANALYSIS                                4
RESULTS AND DISCUSSION—PATHOLOGY                                    5
SUMMARY                                                              6
APPENDIX
DISTRIBUTION
                                  11

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                      LIST OF TABLES AND FIGURE
Table A-l.
Table A-2.
Table A-3.
Table A-4.
Table A-5.
Table A-6.
Table A-7.
Table A-8.
Thyroid
Muscle
Rumen Contents
Abomasum Contents
Abomasum Tissue
Liver
Lung
Bone
 7
 7
'8
 8
 8
 9
 9
 9
Figure 1.
Proposed Supplement Test Site and Morey Bench

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                              INTRODUCTION

The Animal Investigation Program (AIP) developed an operational plan to
support the activities proposed for the Supplemental Test Site (STS).
This plan involved the collection of tissue samples from two mule deer
and three beef cattle for radioanalysis and histopathologic studies prior
to Project Faultless.  Each event was to be followed by the collection of
a like number of animals and tissue samples if there was a likelihood
that an exposure to radioactive isotopes had occurred.  Collection of
animals and techniques of radioanalysis were to be the same as those
employed in past studies.*  Tissues submitted for histopathology included
heart, kidneys, liver, lung, striated muscle, spleen, thyroid, and any
other tissue appearing grossly abnormal.  A thin section was taken of
each organ and preserved in formalin prior to submission to Dr. Gordon
Solomon at Colorado State University for microscopic examination.
Just prior to the Faultless Event approximately 5,000 sheep were grazing
10 to 25 miles from Ground Zero.  A U. S. Public Health Service (USPHS)
certified monitor and the AIP veterinarian were stationed with the sheep-
herders prior to and during the event to advise the sheepherders of the
time of the event and document any damages that might occur from ground
shock or venting.  Since there was no venting and only moderate ground
shock involved, no animals were collected following the Faultless Event.
*Southwestern Radiological Health Laboratory Intralaboratory Technical
 Report ITR-11.

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                              PROCEDURES

Arrangements were made with Mr. Grube, owner of the K Ranches, to pur-
chase three head of cattle grazing in the STS area prior to the scheduled
event.  These cattle were then transported to the Nevada Test Site (NTS),
Well #3, USPHS facilities for sacrifice.
As an item of general interest, it should be pointed out that animals
purchased for analysis are frequently culls that are of questionable
value to the owner due to existing gross pathological processes.* Even
though gross pathology is often present, market price is paid for these
animals in order to maintain good public relations with the livestock
producers in the area.
Additionally, permission was obtained from the Nevada Fish and Game com-
mission to collect two mule deer from the Morey Bench Area (Figure 1).
This  area was selected because of its proximity to the test area and also
because it is the winter habitat of large herds of deer.
These animals were shot and dissected according to an established protocol
and  subjected to post-mortem examinations.  Tissue samples were collected
for  radioanalysis and histopathological examination as outlined in
SWRHL ITR-11.  Gamma spectroscopy and/or radiochemistry of the various
biological samples included analysis for 131I, 1Q6Ru5 144ce, 54Mn, 140Ba,
 137cs,  65Zn, 89sr, 90sr, 95zr, and total potassium.**
Tissues collected for radioanalysis were abomasal content, abomasal tissue,
proximal 6" of the femur, liver, lung, muscle, rumen contents, thyroid,
and  hock joint.
Tissues collected for histopathology were heart, kidneys, liver, lung,
muscle, spleen, thyroid, and any tissues which appeared grossly abnormal.

*Cancer eye (squamous cell carcinoma) and physical deformities.
**Potassium-40 is found in physiologically normal tissues at a fixed per-
   cent  (0.0118) with stable potassium (K).  Within this report, potassium
   values are calculated from the measured radioactive potassium using the
   0.0118 ratio and are recorded as total potassium.

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  Proposed Test Site







  Morey Bench
Figure 1.  Proposed Supplemental Test Site and Morey Bench.



                             3

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                 RADIQANALYSIS RESULTS AND DISCUSSION

Radioanalysis of the tissues from the mule deer and the cattle revealed
very low or nondetectable levels  of all  radionuclides  except for stron-
tium-89 and -90 in the tarsal  and femoral  bones of the respective species
and 1311 in the thyroids of the deer (Appendix A).  The greatest variety
of radionuclides occurred in the  rumen and abomasum contents (Table A-3
and A-4).  Rumen contents yielded 131l in  the deer, 144ce in two cows
and the deer, 14°Ba in the deer,  l°6Ru in  one deer,  65zn in one cow,
and 95Zr in one deer.   Abomasal content yielded 131l in two cows, 144ce
in a cow and a deer, 140Ba in a cow, l°6Ru in two cows and a deer, l37Cs
in a cow, 65Zn in a cow, and 95zr -jn two cows and a deer.  The abomasal
tissue contained 54^n and 65Zn in one cow  and l°6Ru in another cow
(Table A-5).  The liver contained 54Mn and 95zr in one cow and !37Cs in
a deer  (Table A-6).  Muscle contained ^Ce and 54Mn in one cow each and
137Cs, 65Zn, and 95Zr in a deer (Table A-2).   There were no radionuclides
present in any of the lungs analyzed (Table A-7).  Femoral bone samples
from the cows and tarsal bones from the deer--all revealed the presence
of strontium-89 and -90 (Table A-8).  With the exception of 131l, radio-
nuclides values were equal to or  below those reported  in tissues col-
lected during routine slaughters  of cattle from the Knoll Creek, Delamar
Valley, and NTS areas and deer collected on the NTS.  The source of the
131I found in the deer thyroids is thought to have come from atmospheric
fallout resulting from non-United States nuclear tests conducted on
24 December 1967.*
The levels of radioactive isotopes reported in these animals have little
significance other than baselines for comparison with  animals collected
following events conducted on the Supplemental Test Site.  Should there
be a significant delay prior to further testing, additional animals should
be collected to determine if any  change has occurred in the levels of
isotopes reported from this sampling especially in view of the higher
than expected 131l levels.

*Southwestern Radiological Health Laboratory Air Surveillance Network,
 January 1968 Air Results.
                                   4

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                   PATHOLOGY RESULTS AND DISCUSSION

Pathologic lesions occurring in these animals were consistent with pre-
viously reported lesions found during other routine slaughters (BER-8 and
BER-18).  The observed lesions (see Appendix B) can be attributed to
advanced age, natural disease processes, or traumatic injury.
Lesions in the deer were limited to sarcosporidiosis in skeletal  and
cardiac muscles and focal atelectasis in the lungs.  The only gross
lesion* observed at the time of necropsy was pleural adhesions to the
right lobe of one lung.
Significant gross lesions in the cattle included ecchymotic hemorrhages
in the lungs, fibrous adhesions between the liver and diaphragm,  gall
stones, subcutaneous botfly larvae, and a cystic kidney.  Microscopic
lesions included sarcosporidiosis in cardiac and skeletal  muscles;
pyelonephritis and several  foci of lymphocytic infiltration suggestive
of lymphosarcoma in one kidney; microcysts, fibrosis, and  mineralization
of collecting tubules in another kidney; foci of lymphocytic accumula-
tions and focal atelectasis in the lungs; focal hypertrophy and hyper-
plasia of reticuloendothelial cells in the liver; and squamous cell
carcinoma of the cornea! epithelium.
*With the exception of gunshot damage.

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                               SUMMARY

Animals collected on the STS were subjected to thorough radioanalytic
and histopathologic examinations.  Results indicate that these animals
are comparable to animals collected during other routine sacrifices--
both in pathologic lesions present and detectable body burdens of
selected radioisotopes--except for the increased level of ^lj -jn the
thyroids of the deer and one of the cows.  A tentative explanation of
this exception could possible be a result of fallout from Non-United
States nuclear tests.  Presence of the other radioisotopes was erratic
and only slightly above background levels.  The pathologic lesions
observed were attributable to degenerative and/or acquired conditions
commonly seen with advanced age, traumatic injury, metabolic disturb-
ances, or infection.
Since  (as expected) there was no release of radioactivity from the
Faultless event, no animals were collected following the event for
post-mortem analysis.

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                               APPENDIX





Appendix A     Radioanalysis Results — Bovine and Mule Deer



Appendix B     Necropsy Protocols

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                               APPENDIX A


             RADIOANALYSIS RESULTS-BOVINE AND MULE DEER
Table A~l.  Thyroid.
                    144Ce   54Mn   140Ba   106Ru 137Cs  65Zn   95Zr     K
 AIP NO. _ pCi/g  pCi/g  pCi/g  pCi/g  pCi/g  pCi/g  pCi/g  pCi/g  g/g
BOV-1-N14-68
BOV-2-N14-68
BOV-3-N14-68
MD-1-N14-68
MD-2-N14-68
ND
8.45
ND
148
204
ND
ND
ND
ND
ND
1.36
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND = Nondetectable
Table A-2.  Muscle.
AIP NO.
BOV-1-N14-68
BOV-2-N14-68
BOV-3-N14-68
MD-1-N14-68
MD-2-N14-68
pCi/g
ND
ND
ND
ND
ND
l44Ce
pCi/g
ND
ND
0.043
ND
ND
54Mn
pCi/g
ND
0.051
ND
ND
ND
l40Ba
pCi/g
ND
ND
ND
ND
ND
106Ru
pCi/g
ND
ND
ND
ND
ND
13/Cs
pCi/g
ND
ND
ND
ND
0.092
6bZn
pCi/g
ND
ND
ND
ND
1.84
9bZr
pCi/g
ND
ND
ND
ND
0.065
K
g/g
0.0048
0.0031
0.0035
ND
0.0032
ND = Nondetectable

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Table A-3.  Rumen Contents.
AIP NO.
BOV-1-N14-68
BOV-2-N14-68
BOV-3-N14-68
MD-1-N14-68
MB-2-N14-68
pci/g
ND
ND
ND
0.101
0.100
144Ce
pCi/g
0.585
0.065
ND
0.904
1.990
b4Mn
pCi/g
ND
ND
ND
ND
ND
140Ba
pCi/g
ND
ND
ND
0.071
0.111
iUbRu
pCi/g
ND
ND
ND
ND
0.246
pCi/g
ND
ND
ND
ND
ND
pCi/g
ND
2.59
ND
ND
ND
95 Zr
pCi/g
ND
ND
ND
ND
1.12
K
g/g
ND
0.0010
ND
0.0019
ND
ND = Nondetectable
Table A-4. Abomasum
AIP NO.
BOV-1-N15-68
BOV-2-N14-68
BOV-3-N14-68
MD-1-N14-68
MD-2-N14-68
pCi/g
0.071
0.209
ND
ND
ND
Contents.
144Ce
pci/g
ND
1.35
ND
2.25
ND
54Mn
pCi/g
ND
ND
ND
ND
ND
140Ba
pCi/g
ND
0.095
ND
ND
ND
106Ru
pCi/g
ND
0.221
0.105
0.484
ND
137Cs
pCi/g
ND
0.224
ND
ND
ND
65Zn
pCi/g
ND
1.95
ND
ND
ND
95Zr
pCi/g
0.149
0.097
ND
1.89
ND
K
g/g
0.0014
0.0135
ND
0.0029
ND
ND = Nondetectable
Table A- 5. Abomasum
AIP NO.
BOV-1-N14-68
BOV-2-N14-68
BOV-3-N14-68
MD-1-N14-68
MD-2-N14-68
pCi/g
ND
ND
ND
ND
ND
Tissue,
144Ce
pCi/g
ND
ND
ND
ND
ND

b4Mn
pCi/g
ND
0.057
ND
ND
ND

14UBa
pCi/g
ND
ND
ND
ND
ND

106Ru
pCi/g
ND
ND
0.154
ND
ND

137Cs
pCi/g
ND
ND
ND
ND
ND

65Zn
pCi/g
ND
0.081
ND
ND
ND

95Zr
pCi/g
ND
ND
ND
ND
ND

K
g/g
ND
0.0015
ND
ND
ND
ND = Nondetectable
                                   8

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Table A-6.  Liver.
AIP NO. pCi/g
BOV-1-N14-68 ND
BOV-2-N14-68 ND
BOV-3-N14-68 ND
MD-l^N14-68 ND
MD-2-N14-68 ND
ND = Nondetectable
Table A-7. Lung.
AIP NO. pCi/g
BOV-1-N14-68 NA
BOV-2-N14-68 NA
BOV-3-N14-68 ND
MD-1-N14-68 ND
MD-2-N14-68 ND
NA = Not Analyzed
ND = Nondetectable
Table A-8. Bone.
AIP NO.
BOV-1-N14-68
BOV-2-N14-68
BOV-3-N14-68
MD-1-N14-68
MD-2-N14-68
144^ 54
Ce Mn
pCi/g pCi/g
ND 0.048
ND
ND
ND
ND


144
Ce
pCi/g
NA
NA
ND
ND
ND


89Sr
3.672
2.173
3.783
2.461
2.899
ND
ND
ND
ND


54Mn
Pci/g
NA
NA
ND
ND
ND


pCi/g
± 0.
± 1.
± 1.
± 1.
± 1.
pCi/g pCi/g
ND ND
ND ND
ND ND
ND ND
ND ND


140Ba 106Ru
pCi/g pCi/g
NA NA
NA NA
ND ND
ND ND
ND ND


Bone Ash
853*
275
197
064
648
I37rc 657n
Cs Zn
pCi/g pCi/g
ND ND
ND ND
ND ND
0.058 ND
ND ND


137Cs 65Zn
pCi/g pCi/g
NA NA
NA NA
ND ND
ND ND
ND ND


90Sr pCi/g
5.809 ± 0.
14.532 ± 0.
15.473 ± 0.
9.877 ± 0.
23.217 ± 0.
pCi/g
0.049
ND
ND
ND
ND


9bZr
pCi/g
NA
NA
ND
ND
ND


Bone
461*
715
710
625
993
K
g/g
0.0026
0.0023
0.0023
0.0029
ND


K
g/g
NA
NA
0.0024
ND
ND


Ash





* Counting error

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                           APPENDIX B

                          NECROPSY PROTOCOLS
1.   AIP No.  BOV-1-N14-68
    a.   History.   This animal  was born and spent its entire life on the
    Site "C"  Supplemental  Test Site open range area.
    DATE OF DEATH:  10 Jan.  1968      HOUR:  0900    LOCATION:   Well 3, NTS
    DATE OF NECROPSY:   10  Jan. 1968   HOUR:  0930    LOCATION:   Well 3, NTS
    SEX:  Male                        AGE:  1 year
    SPECIES:   Bovine                  BREED:   Hereford

    b.   External  Appearance.   This is a long  yearling Hereford  steer in
    poor physical condition.   Both hind feet  are deformed at their distal
    ends.  The horny covering of the third and fourth digits of the left
    hind foot are missing  and have been replaced by a fibrous pad.  The
    horny coverings of the third and fourth digits of the right hind foot
    have a convex shape.   Due to this abnormal shape, the animal walked
    on the volar surface of the sole near the area of the digital cushion
    resulting in a heavy fibrous covering in  this area.

    c.   Respiratory System.   Diffuse ecchymotic hemorrhages are seen in
    the right and left apical  and cardiac lobes.

    d.   Miscellaneous.   Hypoderma bovis larvae are found in the sub-
    dermal tissue of the back.

    e.   Necropsy Results.
        (1) Liver:  There  is  hypertrophy and  focal hyperplasia  of the
            reticuloendothelial cells.
        (2) Kidney:  Focal accumulation of lymphocytes interstitially
            which is suggestive of early lymphosarcoma.
                                  10

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2.   Alf No. BOV-2-N14-68
    a.  History. This animal was born and spent its entire life on the
    Site "C" Supplemental Test Site open range area.
    DATE OF DEATH:  10 Jan. 1968      HOUR:  0900    LOCATION:   Well  3, NTS
    DATE OF NECROPSY:  10 Jan. 1968   HOUR:  1015    LOCATION:   Well  3, NTS
    SEX:  Female                      AGE:  13 years
    SPECIES:  Bovine                  BREED:  Hereford

    b.  External Appearance.  This is a mature, nonlactating Hereford
    cow in poor physical condition.  Carcinomas are seen on the cornea
    of both eyes.

    c.  Digestive System.  There are extensive fibrous adhesions between
    the peritoneal surface of the liver and the serosa of the diaphragm.
    Approximately 10 small stones, 5 mm in diameter,  are seen in the
    gall bladder.

    d.  Genitourinary System.  The uterus contains a fetus which is in
    approximately the third month of gestation.

    e.  Necropsy Results.
        (1)  Cardiac Muscle:  Moderate sarcosporidiosis  is  present.
        (2)  Kidney:  Multiple  foci  of accumulated  immature  and  mature
            lymphocytes suggestive  of lymphosarcoma.  There  are focal
            areas of proximal  pyelonephritis characterized  by the
            presence of polymorphonuclear  leukocytes  in the  tubules.
        (3)  Striated Muscle:   There is slight sarcosporidiosis.
        (4)  Liver:  There is focal  hypertrophy  and  hyperplasia  of
            reticuloendothelial cells.
        (5)  Eye:  Squamous  cell carcinoma which originates  from the
            corneal epithelium.

                                 11

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3.   AIP No.  BOV-3-N14-68

    a.  History.  This animal  was born and spent its entire life on the
    Site "C" Supplemental Test Site open range area.

    DATE OF  DEATH:  10 Jan.  1968     HOUR:  0900        LOCATION:   Well  3,  NTS
    DATE OF  NECROPSY:  10 Jan.  1968   HOUR:  1100        LOCATION:  Well 3, NTS
    SEX:  Female                     AGE:   14 years
    SPECIES:  Bovine                 BREED:   Hereford

    b.  External Appearance.   This is a  mature Hereford cow in poor
    physical condition.


    c.  Digestive System. Extensive  fibrous  adhesions  are seen between
    the peritoneal surface of  the diaphragm and the serosa of the liver.

    d.  Genitourinary  System.   The kidney  contains  two  small  fluid-filled
    cysts approximately  3 mm in diameter.  Tissues  of these areas are
    submitted for histopathologic study.

    e.  Necropsy Results.

        (1)  Striated Muscle:   There is moderate  sarcosporidiosis.
        (2)  Cardiac  Muscle:  There  is moderate  sarcosporidiosis.
        (3)  Kidney:  There are  several foci of  fibrosis  or chronic
            nephritis.   In addition,  there are microcysts  characterized
            by the absence of  kidney  parenchyma  and  the  crowding  to
            together of  peripheral  structures.   There is also mineral-
            ization  of many collecting tubules.
        (4)  Lung:  There  are foci of  lymphocytic accumulations  sub-
            pi eurally and within  septa.
                                 12

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4.  AIP No. MD-1-N14-68
    a.  History.  This animal was collected in the Morey Bench area.
    DATE OF DEATH:  16 Jan.  1968          HOUR:  2130
                                          LOCATION:  4-Mile Canyon  (STS)
    DATE OF NECROPSY:  16 Jan. 1968       HOUR:  2130
                                          LOCATION:  4-Mile Canyon  (STS)
    SEX:  Female  AGE:  4 years    WEIGHT:  125 Ib.   METHOD:  Estimation
    SPECIES:  Odocoileus heminus           COMMON NAME:  Mule Deer

    b.  External Appearance.  This is a female deer in good condition.
    The left humerus is fractured (due to bullet).

    c.  Genitourinary System.  The uterus contains two fetuses estimated
    to be in the first trimester of gestation.

    d.  Respiratory System.  There are diffuse ecchymotic hemorrhages
    throughout all lobes of the lung.   The left apical  lobe is macerated
    by a bullet.

    e.  Necropsy Results.
        (1)  Lung:   Extensive alveolar  hemorrhage  and free blood within
            the bronchioles.
        (2)  Skeletal  Muscle:  Slight sarcosporidiosis.

5.   AIP  No.  MD-2-N14-68
    a.   History.   This animal was  collected  in the Morey Bench area.

    DATE OF  DEATH:   17 Jan.  1968     HOUR:   2000
                                    LOCATION: 4-Mile  Canyon (STS)
                                   13

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DATE OF NECROPSY:  17 Jan.  1968   HOUR:  2000
                                   LOCATION:  4-Mile Canyon (STS)
SEX:  Female   AGE:  2 1/2 years     WEIGHT:  100 Ib.
                                     METHOD:  Estimation
SPECIES:  Odocoileus hemionus        COMMON NAME:  Mule Deer

b.  External Appearance.   This is a female deer in good condition.

c.  Genitourinary System.  The uterus contains a fetus estimated
to be in the first trimester of gestation.

d.  Respiratory System.   All lobes of the lung contain diffuse
ecchymotic hemorrhages.   The left apical lobe is completely
macerated by the bullet.   The right lobes of the lung have fibrous
adhesions extending from  their peripheral border to the chest wall

e.  Necropsy Results.
    (1) Lung:   There are  focal areas  of atelectasis.
    (2) Cardiac Muscle:   There is slight sarcosporidiosis.
                              14

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                              DISTRIBUTION

 1 - 20  SWRHL, Las Vegas, Nevada
     21  James E. Reeves, Manager, NVOO/AEC, Las Vegas, Nevada
     22  Robert H. Thalgott, NVOO/AEC, Las Vegas, Nevada
     23  Chief, NOB, DASA, NVOO/AEC, Las Vegas, Nevada
     24  Donald H. Edwards, Effects Safety Division, NVOO/AEC,
           Las Vegas, Nevada
     25  R. C. Emens, NTS Support Office, NVOO/AEC, Mercury, Nevada
     26  Martin B. Biles, DOS, USAEC, Washington, D. C.
     27  John S.  Kelly, DPNE, USAEC, Washington, D. C.
     28  Philip Allen, ESSA/ARL, NVOO/AEC, Las Vegas, Nevada
     29  G. D. Ferber, ARL/ESSA, Silver Spring, Maryland
 30 - 34  Charles  L. Weaver, NCRH, PHS, Rockville, Maryland
     35  Bernd Kahn, NCRH, RATSEC, Cincinnati, Ohio
     36  Northeastern Radiological Health Lab., Winchester,  Mass.
     37  Southeastern Radiological Health Lab.  Montgomery, Alabama
     38  Donald W. Hendricks, Safety Evaluation Div., NVOO/AEC,
           Las Vegas, Nevada
     39  Mail  & Records,  NVOO/AEC, Las  Vegas,  Nevada
     40  Paul  T.  Tueller,  University of Nevada, Reno, Nevada
     41  F. Ward  Whicker,  Colorado State University, Fort  Collins, Colo.
     42  V. R.  Bohman,  University of Nevada,  Reno,  Nevada
     43  Al Jonez, U.  S.  Bureau  of Reclamation, Boulder City,  Nevada
     44  District Ranger,  U.  S.  Forest  Service, Las Vegas, Nevada
     45  Region Supervisor,  Nevada Fish & Game  Commission, Las Vegas, Nev.
     46  Director, Nevada  Fish & Game Commission, Reno, Nevada
     47  R. S.  Davidson,  Battelle Memorial  Institute, Columbus,  Ohio
48 -  49  DTIE,  U.  S.  AEC,  Oak Ridge, Tennessee

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