.STUDY OF RUM DISTILLERY WASTE
          TREATMENT AND BY-PRODUCT
           RECOVERY TECHNOLOGIES
               SCS Engineers
       Long Beach, 'Cal ifornia 908.07
         Contract Number 68-03-2573
              Project Officers

                Harry Thron
               Kenneth Dostal
               Donald Wilson
   Industrial  Pollution Control  Division
Industrial  Environmental  Research Laboratory
    U.S.  Environmental  Protection Agency

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                            CONTENTS
                                                            Page
Figures                                                      i i "•
Tables                                                        iv
    I.    Conclusions                                        1-1
   II.    Recommendations                                   II-l
  III.    Introduction                                     III-l
   IV.    General Approach                                  IV-1
    V.    Disposal to a Public Owned Treatment Works         V-l
            (POTW)
   VI.    Ocean Disposal                                    VI-1
  VII.    Land Disposal                                    VII-1
 VIII.    Evaporation                                     VIII-1
   IX.    Pollutant Reduction- through Process               IX-1
            Modifications
    X.    Aquaculture                                        X-l
   XI.    Biological Treatment Systems                      XI-1
  XII.    Bibliography                                     XII-1
Appendices
    A.    Aquaculture Correspondence                         A-l
    B.    Anamet Plants                                      8-1

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                               FIGURES

 Number                                                      Page

  VI- 1     Proposed ocean outfall.   San Juan, Puerto        VI- 8
              Rico (Bacardi)

  VI- 2     Proposed outfalls for Puerto Rico Distillers,    VI-10
              Arecibo

  VI- 3     Proposed outfall for VIRTU'"   ...   '  .      '     VI-12

 VII- 1     Artist's conception of land cultivation         VII-27
              si te

 VII- 2     Representative site profile                     VII-35

VIII- 1     Potassium Salt Recovery  from Crude Potash      VIII-32

VIII- 2     Process flow diagram for the production, of
              org'anic compound fertilizer

  IX- 4     Molasses centri fugat.ion                           IX- 1

  IX- 2     Yeast recycling by centrifugation of             IX- 2
              fermenter beer

  IX- 3     Removal of fermenter bottoms and yeast           IX- 9

  XI- 1     Typical Anamet System                            XI-10

  XI- 2     Yeast Cultivation in Molasses Distillery         XI-17
              Stillage
                                 11

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                             TABLES

Number                       •                               Page

VII- 1     Composition of Selected Constituents in         VII-13
             Mostos on a Dry-Weight Basis

VII- 2     Annual  Quantities of Mostos Generated by        VII-14
             Bacardi, Puerto Rico Distillers, and VIRIL

VII- 3     Estimated Annual  Mostos and Irrigation          VII-16
             Water Application Rates for Bacardi
             (Tultitlan , Mexico) and Serralles
             (Mercedita, Puerto Rico)

VII- 4     Estimation of Maximum Allowable Heavy Metals    VII-18
             and Sludge Loadings for Agriculture Crops
             and Mostos Loadings for Land Farming

VII- 5     Estimated Site Life Based on Annual             VII-20
             Application Rate of 12 ac-in/yr

VII- 6     Minimum Estimated Land Requirements for         VII-24
             Bacardi, Puerto Rico Distillers and VIRIL
             as a  Function of Application Rates

VII- 7     Basis for Conceptual Design                     VII-26

VII- 8     Conceptual Design Waste and Site Parameters     VII-34

VII- 9     Conceptual Design Vehicle Requirements for      VII-37
            .. Land  Farming Utilizing Tanker Trucks for
             Mostos Transportation

VII-10     Labor Requirements for Land Farming Utilizing   VII-37
             Tanker Trucks for Mostos  Transportation

VII-11     Labor Requirements for Land Farming Utilizing   VII-38
             a Pipeline for  Mostos Transportation

VII-12     Capitalists for Three Conceptual land         VII-39
             Farming Sites Utilizing Tanker Trucks for
             Mostos Transportation
                                 IV

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 TABLES (continued)


 Number                                                      Page

 VII-13     Capital  Costs for Three Conceptual  Land         VII-42
              Farming Sites Utilizing a Pipeline for
              Mostos Transportation

 VII-14     Annual  Operation and Maintenance Costs for      VII-44
              Three  Larid Farming Sites Utilizing Tanker
              Trucks for Mostos Transportation

 VII-15     Annual  Operation and Maintenance Costs for      VII-45
              Three  Land Farming Sites Utilizing a
              Pipeline for Mostos Transportation

 VII-16     Summary  of Annual Capital and Operating         VII-46
              Total  Costs for Tanker Truck vs.  Pipeline
              Mostos Transportation

 VII-17     Annual  Land Disposal Costs of Mostos as a       VII-43
              Function of Rum Production

VIII- 1     Mean Chemical Composition of Cane CMS          VIII-18

VIII- Z     Amino Acid Analysis of Cane CMS       .         VIII-19

VIII- 3   ,-. Estimated Maximum Annual Quantities of CMS     VIII-22
              Which  Could be Incorporated into  Puerto
              Rican  Mixed Animal Feed'

VIII- 4     Estimated Annual Potassium Production as an    VIII-34
              Incineration By-Product

VIII- 5     Puerto  Rican Annual Commercial Consumption     VIII-35
              of Potash and Potassium Compounds in
              Direct Application Materials

  IX- 1     Cost Estimates for Pollutant Reduction           IX-V1
              Through Process Modifications, from Puerto
              Distillers and VIRIL

  IX- 2     Breakdown of Cost Estimates for Pollutant        IX-12*
              Reduction Through Process Modifications,
              from  Puerto Rico Disti11ers and VIRIL
                  /*
  XI-12     Recommended Operating Parameters for             XI-12
              Anaerobic Digestion in the Anamet System

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                         I.   CONCLUSIONS

GENERAL
     •  Initial  investigations showed the following four technolo-
        gies are  not  applicable  for  the. treatment  of  mostos:
        - Coagulation and sedimentation
        - Foam separation
        - High gradient magnetic separation
        - Reverse osmosis
     •  In-depth .investigation showed that the following technolo
        gies are also not applicable:
        - Discharge of mostos to a POTW (on Puerto Rico and St.
          Croix)
        - Aquaculture
     •  Land farming, the Anamet process, evaporation, inci-
        neration and organic fertilizer production, appear to be
        feasible technologies.  Their direct application to the
        treatment of mostos, however, has not been fully demon-
        strated.
     •  Agricultural utilization of mostos on sugar cane as cur
        rently practiced by  Serralles Oestileria in Mercedita,
                 ^
        Puerto Rico, and Bacardi in Tultitlan, Mexico, appears
        to be a  viable disposal  alternative.
                              1-1

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     •  Aerobic biological processes such as activated sludge
       *
        and trickling filters are not applicable when considered
        solely by themselves.
     0  Anaerobic digestion  is partially effective in reducing
        BOD and suspended solids.
     •  Pilot plant tests of the Anamet and evaporation processes
        at Puerto Rico Distillers and Bacardi, respectively, had
        not been finished prior to the completion of this study.
        Consequently, conclusions could not be developed with
        respect to their individual  performance.
     •"  The economics of mostos treatment and disposal  technology
        are far from  being clearly defined. The present practice
        of discharging untreated mostos into the ocean through
        short outfall pipes  is inexpensive.  All other treat-
        ment or disposal  alternatives investigated are by con-
        trast very costly.   For example, estimates for land farm-
        ing ranged from approximately 8 to 16 cents per proof gal
        Ion produced. The cost of.treatment and disposal  by the
        evaporation (CMS) production process, the Anamet treat-
        ment process, and incineration were not determined. It..
        is hoped that the pilot plant research presently under
        way will  provide  accurate cost ranges.
TECHNOLOGY SPECIFIC
     •  Land Disposal
        -  Land reclamation does not  appear to be a viable  dis-
          posal  option
                               1-2

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- Agricultural  utilization appears to be a practical
  disposal  option for Puerto Rico Distillers.
- The combination of potentially available land and dry
  climate, suggests that VIRIL is suitably located to
  utilize land  farming as a disposal  option
- Bacardi is less favorably situated  and may not be able
 .to locate, sufficient land near their Catano facility
  to make transport of mostos to the  disposal site eco-
  nomically feasible.
Evaporation
     A review of available literature indicates that
evaporation of  mostos to condensed molasses solubles
(CMS) is a  technologically feasible treatment alternative
The utilization or ultimate disposal  of CMS, however, may
not be economically practical.
     Estimates  of local (Puerto Ricanl CMS consumption
suggest that approximately 18 percent of the total  theo-
retical CMS production could be utilized as an animal
feed supplement if incorporated in the feed at the five
percent, by weight, level.                            '.
Incineration
     The recovery of potassium salts  from incinerator asr.h
is  estimated to result in an annual  supply of 5,550 T of
        t»
<20, which  is approximately five times greater than the
current (1965-1975) average annual  consumption of direct
                       1-3

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application materials.  The total potassium market is
uncertain.
Organic fertilizer production
     Utilizing CMS as a raw material, an annual estimated
53,500 metric tons of an organic N-P-K fertilizer could
be produced by the three distillers.  The annual local
consumption of this type of fertilizer was not available
from the literature reviewed.
Biological treatment processes
     Available data suggests that, while neither aerobic
or anaerobic processes are, in themselves, satisfactory
treatment alternatives, the combination of the two pre-
sent technologically feasible treatment options.  One
such process known as Anamet, has been successfully
utilized on waste streams similar but not identical to
mostos.  This suggests that other similar combined bio-
logical systems,  while not specifically investigated
during this study, may have applications to the treatment
of mostos.
                      1-4

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              II.  RECOMMENDATIONS

Results of the Anamet and CMS pilot plant tests should
be incorporated into any future evaluation of rum wastes.
A program should.be initiated to evaluate the potential
for land farming of mostos.  Pilot studies should be con-
ducted in Puerto Rico and St. Croix to determine:
- Degree of mostos pH adjustment required
- Compatibility of land farming techniques with site
  specific topographical and hydrological conditions
- Disposal application rates as a function of climate,
  methods, of application, so'lid.s loading, etc...
- Degree of mostos degradation as a function of time
- Accumulation of mostos constituents within the soil
  matrix and the extent of cationic and anionic migration
  through the 'soil column
- Potential aesthetic problems.
The feasibility of mostos discharge by Puerto Rico Dis-
tillers to nearby government-owned sugar cane fields
should be investigated.
          , •
The Bacardi Corporation should consider a fusel oil  and
"heads" recovery program, thus eliminating this compo-
nent from the mostos stream.
                      II-l

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Recent innovative aerobic biological treatment processes
should be investigated for potential use following
anaerobic digestion.  Included among these are:
- UNOX, a proprietary pure oxygen activated sludge pro-
  cess developed by Union Carbide
- Deep Shaft, developed by Eco-Research
- Carrousel, developed by Envirotech.
The potential for local  (Puerto Rican)  and export con-
sumption of CMS should be fullly investigated.
A study investigating the short and long-term markets
for local organic fertilizers consumption should be
initiated.
A study should be initiated to determine the total
potassium market for Puerto Rico and St. Croix.
                      II-2

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                     III.  INTRODUCTION

     On December 6, 1977, the United States House of Representa-
tives presented a mandate to  the United States Environmental Pro-
tection Agency (EPA) to study the compatibility of pollutants dis-
charged from Puerto Rico and  Virgin- Islands rum distillers with
the marine environment. Specifically, the EPA was to determine:
     •   The environmental  acceptability of rum distillation
        wastes
     •   The potential  benefits  or adverse  effects of marine
        di sposal
     «   The geographical,  hydro!ogtcal , and biological  charac-
        teristics of the marine receiving  waters around Puerto
        Rico and the Virgin  Islands
     •   Waste processing technologies  which facilitate the
        recovery of nutrients and/or other beneficial  materials
        in the wastes
     .•   Treatment technologies  which reduce the discharge of rum
        distillation waste pollutants.
     In 1973, the EPA  initiated a study program aimed at fully
                    t»
assessing the opti.ons  for disposal  of  rum  distillery wastes and
associated environmental consequences.   The primary objectives
of this study are (1)  to identify and  assess various treatment
                               III-l

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technologies and by-product recovery options available to the rum
distillers, and (2) to indicate the existence of information
voids so that research and development efforts can be directed
toward the filling of these gaps.
                              III-2

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                     IV.   GENERAL APPROACH

     Selection of treatment and by-product technologies for ini-
tial  investigation was  the first project objective.  An initial
list  of treatment and by-product recovery technologies was pre-
pared from a variety of sources, including:
     •  In-house EPA information on previous and related work
     •  Interviews with personnel from The Bacardi  Corporation,
        Puerto Rico Distillers, and The Virgin Islands Rum Indus-
        tries, Limited  (VIRIL)
     •  Intensive literature review of available foreign and
        United States research  projects dealing with the treat-
        ment of cane molasses distillation residues and related
        wastes and processes
     •  Unpublished and in-press data made available by the rum
        di s.ti 11 ers
     •  Current university research projects
     •  Interviews and  correspondence with knowledgeable consul-
        tants, and industrial equipment manufacturing represen-
        tatives.
                   /*
     This assemblage of information and data resulted in the
following list of potential treatment and by-product recovery
technologies for preliminary evaluation.
                               IV-1

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Treatment of wastes by a public owned treatment works
(POTW)
Biological treatment processes
- Aerobic
    •  Trick!i ng f i1ter
    •  Activated sludge
- Anaerobic
    •  Anaerobic digestion
- Anaerobic/aerobic systems
    •  Coscul1euela
    t  Anamet
- Other
    •  Cultivation of fodder  yeasts, bacteria, and fungi
Evaporation
- Production of condensed molasses solubles (CMS) for
  animal  feed
- Utilization of CMS as an intermediate product
    •  Incineration with conventional ash disposal
  •  •  Incineration with potassium recovery
    •  Organic fertilizer production
Land disposal
-Agricultural utilization
- Land reclamation
         /•
- Land farming
Process modifications
- Molasses pretreatment
                       IV-2

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        - Removal  of fermenter beer
        - Recovery of fusel oils and heads
        - Removal  and recovery of yeast from fermenter beer
     •  Ocean disposal
     ••  Aquaculture
     •  Physical/chemical treatment processes
        - Coagulation and sedimentation
        - Foam
        - High- gra.dfent magnetic separation
        - Reverse  osmosi s.
SELECTION PROCESS
     While each of the above treatment and by-product recovery
technologies may be considered as a potentially viable alternative
to the current waste discharge problems of the rum distillers,
some of the alternatives have greater application potential than
others.  Most of the technologies have never been applied  to rum
wastes., although they have been' successfully employed for  the
treatment of similar high strength fermentation wastes.  Fur-
thermore, the time constraints of this investigative effort dic-
tated that the major research efforts be directed toward only
those promising technologies.  With these factors in mind, the
following criteria were adopted to evaluate each alternative:
                                                                 .*•>
     •  Extent and ,substance of available literature
                  f* " ~
     9  Direct application experience of the technology to the
        treatment  of rum distillery wastes
                               IV-3

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     •  Direct application experience of the technology to the
        treatment of similar wastes - (e.g. beet molasses fer-
        mentation residuals)
     •  Performance and operating characteristics of the treat-
        ment process on high strength waste streams
     t  Estimated investment and annual  O&M costs
     •  Energy consumption
     0  Potential for retrofitting technology to existing plant
        facilities
     0  Ultimate disposal  of primary and secondary wastes - (e.g
        final effluent, sludge, ash).
     Other considerations  also entered into the evaluation pro-
cess.  For example, the fact that the distillers are located on
islands considerably removed from the contiguous states and the
limited availability of land and other resources were important
factors.
      Each technology was  qualitatively reviewed  in  terms of the
above  criteria and  either accepted or rejected  for  more  inten-
sive  investigation.
TREATMENT TECHNOLOGIES AND  BY-PRODUCT RECOVERY  OPTIONS
CONSIDERED UNFEASIBLE
      The technologies considered unfeasible are:
      0  Sedimentation and coagulation
      0  Foam  separation
      0  High  gradient magnetic  separation
      0  Reverse  osmosis.
      In the  following section,  an explanation is given for the
rejection of  each of these  technologies.
                                IV-4

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Sedimentation and Coagulation
     Sedimentation for treating rum distillery waste was found
to be unsatisfactory by Biaggi (Jl)-due to the fact that almost
all  of the waste solids were in solution.  Siaggi (11) and Sen
et al . (.92) reported no success with the chemical coagulation
process a.s a means- of treating rum waste.  Even with extremely
high dosages of ferric chloride, aluminum sulfate, lime, ben-
tonite, and various other coagulants, no floe formation was
observed.
Foam Separation
     Foams may be used to remove substances dispersed in a liquid
by several processes known collectively as foam separation- (83).
The common feature of all these processes is that separation is
brought about by virtue of differences in the surface activity
of the dispersed substances.  In contrast to most other treat-
ment processes, foam separations work most efficiently with dilute
solutions.
     Air or other gases bubbling through the liquid generate gas-
liquid interfaces and result in foaming, which allows the inter-
faces to be collected.  Surface-active substances are adsorbed
onto the rising bubbles and concentrate in the foam.  The sub-
stances then can be removed from the liquid.
     Foam techno! ojj'i es applicable to the treatment of mostos
                 /•
include froth flotation for the removal of suspended solids,
and foam fractionation for the removal of certain ionic species
(metals , etc) .
                               IV-5

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     Certain problems and shortcomings are inherent to the use
of foam separation for the treatment of mostos.  These are:
     t-  Foam separation technologies do not remove the soluble
        BOO from the waste stream.  Since the principal compo-
        nents of mostos are soluble solids, further treatment of
        the waste stream would be required.
     •  Aerating mostos in a laboratory scale activated sludge
        study has resulted in excessive foaming (11).  The addi-
        tion of gas for foam separation would probably increase
        the foaming problem.
     •  No treatment studies have been completed on the applica-
        tion of foam separation technologies to mostos.  No trans
        fer technology exists for the foam treatment of similar
        wastes.
High Gradient Magnetic Separation (H6MS)
     Magnetic separation techniques have- been used since the
nineteenth century to remove tramp iron and to concentrate iron
ores.  A variety of conventional magnetic separation devices are
in wide use today.  These devices generally separate relatively
coarse particles of highly magnetic material containing large  ;
amounts of iron from non-magnetic media.
     In recent years, magnetic devices have been developed which.  :>
                    /•
are capable of separating even weakly magnetic materials on
the order of one micron in size at high waste stream flow rates.
These so-call.ed "hig-h gradient magnetic separators" have been
                               IV-6

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designed to maximize the magnetic forces on fine, paramagnetic
materials.  The separations may be economically operated at pro-
                                          2
cess rates of up to several hundred gpm/ft  (!)•-
     High gradient magnetic separators typically consist of a
canister type device packed with fibrous ferro-magnetic material.
They are magnetized by a strong external magnetic field sur-
rounding the canister.  Magnetic particles contained  in fluids
flushe.d through the canister are trapped on the edges of the
magnetized fibers ;• non-magnetic particles pass through unaffected,
This type of system has been demonstrated to work effectively in
treatment of wastewaters containing low levels of impurities  (1).
     Magnetic components can be separated directly from waste
Streams, whereas non-magnetic contaminants (organics,  colloids,
etc.) must be given a "magnetic handle" by seeding the. stream'
with finely divided magnetic seed material. Binding to the seed
is accomplished by adsorption or coagulation.
     Adsorption mechanisms are not fully understood, but are pos-
tulated to include chelation or surface bonding.  The more
practical  technique is chemical coagulation of  the contaminants
into aggregates.
     The  potential for HGMS as an option for  treating mostos
appears  to be unfavorable  (US'.).  The  system  relies heavily on
the  ability of alum-^or other flocculants to produce aggregate
particulates.  It has been demonstrated by Biaggi (11) and Sen
et  al. (92), however, the  sedimentation was ineffective in treat-
ing  mostos due to the high percentage  of soluble constituents in
                               IV-7

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the waste stream.  Further, the HGMS system seems favorable as
a secondary or tertiary treatment module, handling waste streams
whose soluble and particulate constituents are in relatively low
concentrations.  Mostos, on the other hand, has a soluble solids
content of approximately 10 percent.
Reverse Osmosis
     Reverse osmosis (RO) is a process for removal of dissolved
solids in water and wastewater.  The name is derived from the
fact that, in the process, water is made to flow from a solution
of higher concentration through a semi-permeable membrane to a
solution of lower concentration, the opposite of natural osmosis.
To cause this flow, a pressure greater than the osmotic pressure
of the water or wastewater being treated must be applied.  In-
practice, pressure considerably in excess of the osmotic pres-
sure (.400 psi is a common feed pressure in desalination plants)
is required to obtain a water flux through the membrane,which is
sufficiently large to make the process effective and practical
(25', 26, 70).
     The majority of research and development on RO to date has
been concentrated on applications involving the treatment of
brackish waters.  However, some investigation of the applicabil-
ity of RO to the treatment of municipal  wastewater has also      ':
occurred.  In addit4on, the cost of power and membrane replace-
ment is high.
     Pilot programs of RO treatment of wastewater have been con-
ducted under EPA sponsorship.
                               IV-8

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     In these studies it was found that substantial  pretreatment
was required prior to operation of the RO unit to remove dis-
solved organics, which otherwise fouled membranes.  Pretreatment
included chemical flocculation, coagulation, and filtration plus
activated carbon adsorption.  These constitute a very expensive
pretreatment chain (25, 26, 98)!..  Additionally, RO  technology
has. been generally applied to waste streams in which total  dis-
solved solids (TDS)  were not in excess of 7,000 ppm.  Mostos, on
the other hand,  has  a TDS concentration of 77,,400 to 85,600 ppm
(93).  Therefore, RO could not be implemented without some, form
of dilution, an  option which is not realistic, especially so on
St. Croix, an island which has suffered under drought conditions
over the last several years.  Therefore, reverse osmosis is not
considered to be a feasible treatment technology for. rum distil-
lery wastas.
FEASIBLE TECHNOLOGIES
     The following chapters present an analysis of each treatment
technology and by-product recovery option.  Throughout the text,
rum distillery wastes have been identified by one or more of
several synonyms.  These are:  rum distiller's wastes, rum slops,
slops, and mostos.  They are all defined as the combined liquid
discharge resulting  from fermentation of sugar cane  molasses.
                               IV-9

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              V.   DISPOSAL TO A PUBL-IC OWNED
                   TREATMENT WORKS (POTW)
INTRODUCTION
     Manufacturing facilities located within any of the 48 con-
tiguous states and faced with one of several wastewater treatment
optio.ns generally have a POTW- in reasonably close proximity.   If
all options, prove technologically OP economically unfeasible,
the POTW can be selected as a final choice.  Decision parameters
such as present and future surcharges, peak flows, 300 equivalents,
increased or decreased plant production must necessarily be fac-
tored into the final  selection of treatment alternatives.  The rum
industry on both Puerto Rico and St. Croix.,  the U.S. Virgin
Islands, should, therefore, be examined with discharge to a POTW
as a viable- di sposaT  option.
     The following discussion presents such an examination on  a
site-specific basis.
Bacardi
     The Bacardi Corporation is located approximately 1 1/2 mi  •
northwest of Catano.   It is bounded on the north by Ensenada de  •
                                                                :j:v
Boca Vieja and by the small village of Palo Seco, and to the east
                   i-
by San Juan Say. '•The overall region lacks an adequate sewage
system.  This inadequacy presently causes gross pollution to the
waters of the region  from domestic and industrial wastewater
sources (1).  The Puerto Rico Aqueduct and Sewer Authority (PRASA)
                               V-l"

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has approved the Bayamon Wastewater System to provide sewage ser-
vices to areas of Aguas Buenas, Catano, Toa Baja, Toa Alta, and
San Juan.  The wastewater system to be constructed will consist
of trunk and branch sewers to collect wastewater from local sewer
systems in existing and projected population centers through the
year 2020.  Included in this system is a treatment plant  (POTW)
designed to accomodate the regions expected wastewater.   The POTW
will be located in Barrio Palmas east of the Bayamon river channel
in the municipality of Catano about 1 km from the sea coast of
Ensenada de Boca Vieja.  The plant will be constructed in 10 MGD
modules and in treatment phases.
     The initial phase will  be designed for primary treatment with
average capacity of 40 MGD;  effluent will  flow directly to the
ocean through an ocean outfall to be constructed simultaneously
with the trunk and. POTW systems (5).  Construction has not been
implemented on either the POTW,.or the ocean outfall.  The future
date for upgrading the POTW  to secondary treatment is now known.
     Previous analytical investigations of mostos reported an
average total dissolved solids content of 7.77 percent, represent-
ing approximately 93 percent of the total  solids present  (93). Fur-
ther, mostos is extremely high in soluble BOD and COD, with res-
pective average ranges of 26,500 - 47,400 ppm and 72,000  - 92,00.0
ppm.  The technological considerations of primary treatment are  •'
                   i*
such that they wo,uld not be  expected to remove any significant
quantity of either.  If discharged to the POTW, the Bacardi efflu-
ent would be diluted and discharged essentially untreated to the
ocean.  Therefore, the alternative of discharging to a POTW for
                               V-2T,

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the purposes of pollutant reduction is effectively not available
to Bacardi",
Puerto R'ico Distillers
     Puerto Rico Distillers is located on the western edge of
Arecibo, a city which currently discharges its domestic and  indus-
trial  streams directly to the ocean.  Construction has reportedly
begun on a trunk line system to route domestic and industrial
wastes to a. primary POTW (also under construction) located on
the eastern side of Arecibo @7).  There are reportedly no pre-
sent plans to install a trunk line to the western extremities of
the city - the location of the distillery.  Design capacity,
estimated completion data, future timetable for upgrading to
secondary treatment,, etc.,, were not available in  the  literature
reviewed and from personal correspondence."  Further,  since the
mostos discharged from Puerto Rico Distillers is  virtually iden-
tical  to that of Bacardi, removal of any significant  quantities
of pollutants would not be expected.  Therefore,  discharge to a
POTW for Puerto Rico Distillers is not a viable option.
Virgin Islands  Rum  Industries,  Limited  (VIRTU
     The VIRIL plant is located in a rural area about 5 mi east
of Frederiksted, St. Croix.  A small POTW with primary treatment
is located several  miles east of the distillery,  on the eastern
edge of the airport property.  Recently, a secondary  module  was •**
constructed several hundred yards from the primary facility  and
is currently in operation.  The POTW serves several newly developed
housing areas and  the airport facilities.  Current average flow
is about  0.5 MGO;  design capacity is 4 MGD (113).  Secondary effluent

                               V-3--

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is discharged to the ocean through a 48 in diameter, 9,000 ft
outfall (71).
     VIRIL and POTW personnel have conducted preliminary discus-
sions concerning.the possibility of pumping via pipeline, the
mostos and otherplant wastestreams to the POTW.  The POTW person-
nel  suggested that considering the high strength and waste volume
(population equivalent is approximately 90,000), the only accep-
table method of receiving mostos would be in a diluted state so
that the BOO would not exceed that of domestic sewage (200 - 300
ppm BOD) (71).  Fresh water, sufficient for the necessary dilution,
however, is not available on this water-starved island.
     Dilution with seawater is not possible due to the toxic
effects of sodium chloride on POTW microorganisms.  Therefore,
discharge to the POTW is not a viable alternative to VIRIL.
SUMMARY  '
     Discharge of mostos to a POTW is not a viable or practical
alternative for any of the rum distillers in Puerto Rico and St.
Croix, U.S. Virgin Islands
                              V-4,

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                      VI.   OCEAN DISPOSAL

     Outfall  pipes into the ocean are intended to return contami-
nated fluids  to the environment in a way that promotes adequate
transport and dispersion of the waste fluids.  Urban and industrial
communities who discharge their complex mix of wastes to the
ocean are typically located along the shallow edge of the ocean,
with often tens or even hundreds of kilometers of contaminated
shelf between the shoreline and the deep ocean.   The bottom slope
on the shelf  is typically less than one percent.   Thus,  outfalls
extending several kilometers offshore discharge  into a body of
water of large latera.l extent compared to the depth.
     The decay times-of the wastes are important  in the  choice of
effective discharge strategies.  For example-, the problems of
very persistent contaminants such as DOT cannot  be alleviated by
dispersion from an outfall.  Such wastes must be  contained at the
source and prevented from entering the environment.  On  the other
hand, degradable organic wastes may be effectively disposed of  .
through a we! 1 -desi gned ocean outfall.  Since the typical decay  .-.
                                                                 /
time is only  a few days, potential problems are  only local  and  '&
                   »•
not regional  or global.
     Coastal  waters are biologically productive  because  of the
infusion of nutrients from nearby land masses and upwelling of
nutrient-rich bottom water.  Man's additions of  organic  nutrient
                               VI -1

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material  cause perturbations in the coastal  ecosystems.   Most
past problems have resulted from excessive waste loadings very
close to  shore or in estuaries with poor circulation patterns,
that is,  in cases where man's effects have been overwhelming.
The current practice of outfall design for large systems is to
build long pipelines (>3 k.m) on the open coast to depths up to
70 m, and to install large multiple-port diffusers.   They can
achieve large initial  dilutions of the order of 100  to 1 and
produce submergence of the entire wastewater cloud below the
surface when there is  sufficient ambient density stratification.
This practice has greatly improved local water quality at rela-
ti vely 1ow costs (55).
     A typical outfall  consists of a submarine pipeline  roughly
perpendicular to the shoreline.  In shallower water.it is buried-
under rip-rap for wave protection and, at greater depths,-it is
laid on the ocean floor with ballast rock on either  side.  At the
far end, there is usually a diffuser section, a manifold  with
many small holes to distribute the flow throughout a large ocean
area.
     Effective utilization of the ocean for  assimilation of waste-
water depends on both  engineering and environmental  considerations
Engineering feasibility for ocean diffusion  depends  upon good jet;
mixing and oceanic convection and diffusion.  Important  factors
are ocean currents-, density stratification,  waste decay  constants,
and the use of multiple-jet diffusers.
                               VI-2

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SUMMARY OF CONSIDERATIONS FOR AN OCEAN OUTFALL
     Initial  planning for an ocean outfall  should include an
oceanographic survey in the vicinity of possible discharge sites
to determine  (16):
     1.  Currents (direction, magnitude, frequency,  variation
         with depth, relation to tides, water displacements)
     2.  Densities  (variation with depth determined  from salinity  .
         and  temperature data and standard  tables)
     3.  Submarine  topography, geology, and bottom  materials
                                                  •
     4-.  Marine biology
     5.  Turbidity
     6..  Dissolved  oxygen,, etc..
     The final  site selection for an ocean  outfall  is  usually
based on general  characteristics of the coastal  waters and on
topography of the drainage area.  Details of diffuser  design are
developed after the general site is chosen.
DESIGN FOR DIFFUSION
     Since the objective of an ocean outfall is  to  disperse waste
effluent in the ocean, first consideration  is given  to the analysis
of diffusion.  From such an analysis one establishes a satisfactory
length for the outfall from the  shore, a desirable  diffusor pipe":
arrangement and the approximate  number and  spacing  of  ports.    ..'•
     There are essentially three stages of  diffusion or turbulent"
                   /*
mixing of wastes  discharged into the ocean  (16):
     1.  Initial  jet mixing (considering jet strength, currents,
         and  density differences)
     2.  Development of a homogeneous diffusion  field

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     3.  Turbulent diffusion of waste field as a whole due to
         natural oceanic turbulence.
     An excellent treatment on the  above mentioned stages of
diffusion is presented in papers  by N.  H.  Brooks (16, 17, and 18).
Some of the highlights of these papers  are discussed  here.
Port Design
     The outlet ports may quite satisfactorily be circular holes
in the side of the pipe without nozzles or tubes or other pro-
jecting fittings.  For optimum dilution, the jets should discharge
             *
horizontally, with no initial  upward velocity.  The inside of the
port should be. bell-mouthed to minimize clogging and  to provide
a discharge coefficient which  will  remain  constant over a period
of years.
Number of Ports
     Research has shown that a multiple-outlet diffuser greatly
enhances the possibility of generating  a submerged waste field
(T<6).  if the waste  is discharged at a  single port or "en masse,"
its dispersion and dilution will  be slower than if it is dis-
charged over a large area through a number of ports.   In fact,
without the use of multiple-outlet  diffusers, other conditions
being equal, much longer outfalls into  deeper water are necessary
to provide the same  degree of  dispersion.   In general,  the greater
the depth (volume),  the greater the dispersion.
                   /•
Velocity in Diffus»er
     The flow velocity in all  parts of  the diffuser should be high
enough to prevent deposition of any residual  particles.   For
settled sewage, velocities of  2 to  3 feet  per second  at peak  flow
                               VI-4!

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 are adequate (but borderline).  If deposition takes place in any
 part of the diffuser over an extended period of time, the cross
 section of the pipe may become so constricted that locally the
-velocity will  be reduced, a cycle that will accelerate the in-pipe
 deposition and clogging process...".: "
 Flow Distribution
      The. outflow between the various diffuser ports should be
 fairly uniform'.  If the diffuser is laid on a sloping sea bottom,
 it will be impossible to achieve uniform distribution between
 ports for all  flow rates.  In such cases, it is desirable to
     •,
 make the distribution fairly uniform at low or medium flow,  and
 let the deeper ports discharge more than the average port dis-
 charge during  high rates of flow.
      If waste, pumping is necessary or the available gravity  head
 is limited, the total head loss.in any diffuser should be kept
 reasonably small.  Additional head losses of a few feet are
 usually adequate.
 Prevention of Seawater Intrusion
      All ports should flow full  in order to prevent the intrusion
 of seawater into the pipe.  Seawater entering the pipe will  be
 stagnant and will tend to trap grit and other settleable matter,
 reducing the hydraulic capacity  of the diffuser.
 SITE SPECIFIC  CONSIDERATION
                    ,»
      The fol 1 owing--section presents ocean disposal options
 available to each rum distiller  predicated on the following
 assumptions:
      1.  The chemical and physical  characteristics of mostos are
          such  that ocean disposal  results in minimal  environmental
                                VI-3-

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         harm to local  ocean flora and fauna.
     2.   Practice of removing "heads" and "fusel oils" is con-
         tinued by Puerto Rico Distillers and VIRIL and is
         adopted by Bacardi .
Bacardi  Corporation
     The Bacardi Corporation manufacturing facility (Figure VI-1).
is located just south of the town of Palo Seco, on a peninsula of
land bounded on the east by  San Juan Bay and on the west by both
the Palo Seco Thermoelectric Plant and the Bayamon River.  Approxi-
mately one mile southeast of the plant is the. town of Catano.
The Bayamon River currently  receives its water from the Thermo-
electric Plant (approximately 100 MGO - cooling water) and from
Bacardi  (approximately  0.2 MGD - condensate, boiler blow down,-'
cooling  water, fermenter washings, etc.)
     Design has been submitted and approved for the construction
of the Bayamon Wastewater Treatment Plant (initial average flow
of 27 MGO -primary treatment only), and an ocean outfall  accepting
the combined flow of the proposed Bayamon and existing Puerto
Nuevo Treatment Plants.  The combined effluent will be discharged
through  a 120-in-diameter concrete pipe extending 4,000 ft into \
the Atlantic Ocean to a depth of about 160 ft below mean ocean   ':
sea level.  The wasf.ewater will -be diffused to the ocean through
p.orts located along two 1,000-ft diffusers.  Initial dilution
is estimated to be about 150-175:1.  The outfall pipe (Figure VI-1)
will be  located on the  far eastern edge of the Bacardi property.

                               VI-6

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   .  If Bacardi  were to utilize this newly designed system, two
options would be available:
     Option 1 :   Discharge to the Bayamon Wastewatsr Treatment Plant
     Bacardi  would discharge its combined effluent (approximately
     0.5 MGO) to the proposed primary treatment facility.  The
     Bacardi  effluent would be combined with other domestic and
     industrial  wastes, thus affecting dilution, and would be
     discharged  to the ocean.  However, the nature of mostos. is
     such that  the high 300 and COO loads are a function of the
     high percentage of solubilized organics (nonfermentable
     sugars,  acids, etc.) and would not be appreciably reduced by
     primary  treatment.  Hence, the mostos would essentially pass
     through  the primary system unchanged.
     Option 2:   Discharge directly to the outfall.
     Initial  flow through the outfall pipe is anticipated to be
     40 MGD.   By running pipe across the plant, grounds and
     connecting directly to the outfall pipe, the 0.3 MGD Bacardi
     mostos stream would receive an instantaneous dilution of
     130:1.  Assuming that the diffuser operates within its
     design limits, the Bacardi effluent will be further diluted
                                   *                             • •
     upon discharge to the ocean.  The overall  mostos dilution is;
     estimated  to be 20,000:1.
     The difference between each of the above alternatives is
                   f
basically one of semantics.  Both dilute the waste to the same
degree with final discharge to the ocean.  However, in the first
case, discharge is technically to a POTW; in the latter, discharge
is directly to  the ocean without benefit of "treatment".
                               VI-7

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Figure VI-1.
Proposed ocean outfall
( Bacardi ).
          San Juan, Puerto Rico
VI-8

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Puerto Rico Distillers
     Puerto Rico Distillers is located in the western section of
Arecibo,  bounded on its eastern,  western, and southern borders
by encroaching city development and to the north,  by the Atlantic
Ocean (Figure VI-2).  Two options are presented.
     Option 1:  • 1,600-ft outfall.
     A 6-in-diam'eter concrete  (C  = 130)  pipe will  extend 1,600 ft
     into the Atlantic Ocean  to a depth  of approximately TOO  ft.
     Based on a volume of 0.4  MGD (280 gal/min), effluent would
     flow at a velocity of about  3.2  ft/sec.   Head loss  was  cal-
     culated 'to. be 7.4 ft/1,000 ft or 11.8 ft for  the entire
     1,600-ft outfall.  Pumping requirements  are estimated at
     1.1  brake horsepower (BHP).'
     Option 2:   2,000-ft outfall.
     A 6-in-diame.ter concrete  (C  - 130)  pipe  will  extent 2,000 ft
     into the Atlantic Ocean  to a depth  of approximately 150  ft.
     Based on a volume of 0.4  MGD (280 gal/min), effluent would
     flow at a velocity of 3.2 ft/sec.  Total  head loss  would be
     14.8 ft.  Pumping requirement is estimated  at 1.4 BHP.
     Initial dilution for either  option  is estimated at  50:1.
If the density of mostos could  be  lowered to  less than that of
seawa.ter  (for example, by diluting mostos with other lower
                              VI -9

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           ., ( 156 FT DEEP)
Figure V'I-2.   Proposed outfalls for Puerto  Rico  Distillers,  Arecibo.

-------
specific gravity waste streams), mixing- would be expected to
increase.   If the outfall  is  extended to 3,000 ft,  the waste
stream would be discharged at a depth of approximately 400 ft.
Virgin Islands Rum Industries, Limited (VIRIL)
     The VIRIL plant is located in the southwestern  area of
St. Croix, approximately 1-1/2 mi  east of Long Point.   Speci-
fically, the plant is on the Diamond Estate of Princess Quarters
approximately 1-1/2 mi north of the ocean.   The mostos dis-
charge pi pe,. original ly a  sewer outfall, runs from the plant,
across the estate known as Betty's. Hope, and terminates approxi-
mately 1,000' ft in the ocean at a  depth  of about 15  ft (see
Figure VI-3).. .It is  worthy of  note  that  the  ocea'n  slopes  very
gradually  throughout this  area so  that any outfall pipe designed
for depths of TOO ft is reached, the degree of slope increases
rapidly.  For this reason, only one option has been  examined.
     Option 1:  150-ft outfall.
     An outfall to a depth of approximately 150 ft.   Approxi-
     mately 22,000 ft of 4-in-diameter concrete pipe would be
     required.  VIRIL's estimated  140-gal/min effluent would
    -flow  at 3.5 ft/sec.  Head loss is estimated at  13.6 ft/1,000
     ft or 300 ft for the  entire net fall.  Pumping  requirements,
     would be equivalent to 14.2 BHP.
     Due to the rapid slope of the ocean bottom at the outfall
                   /*
termination point^and the  density  limitations of the waste stream,
diffusion  ports would probably not be required.  Rather, the waste
stream could be permitted  to exit  directly from the  end of the
                              VI-TT

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                                          OUTFALL
                                 (32,000 FT  FROM PLANT)
Figure  VI-3.  Proposed outfall for VIRIL.

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outfall.  Dye studies would have to be performed to investigate
the velocity and direction of the ocean currents to ensure that
the stream would not be diffused into the shallower areas.
     The continuation of mostos disposal  to the ocean will be
largely dependent upon the results of marine biological studies
currently in progress.  Consequently, a complete economic analysis,
including capital and O&M costs for a pipeline, fs premature.
However, to place the economics of disposal in proper perspective
to other disposal technologies, pipeline estimates are presented
for Puerto Rico Distillers and VIRIL.  It is assumed that costs
include pipe, connectors, ballast and/or- cement (for anchoring
pipeline to ocean floor), and labor for installation.  Further,
it is assumed that, the above-mentioned costs for a 6-in-diameter
pipe will be approximately $125/ft and for a 4-i n-diameter plpe,.
$100/ft (46).  Extending these unit costs to the- pipeline lengths
shown in the preceeding section gives the following:
     «  Puerto Rico Distillers
          Option 1  (1 ,600-ft outfall  - 6-in-diameter pipeline)
                    1 ,600 x $125 = $200,000
          Option 2 (2,000-ft outfall  - 6-in-diameter pipe)
                    2,000 x $125 = $250,000
     •  VIRIL
          Option 1  (22,000-ft outfall - 4-in-diameter pipe)
                 /'22,000 x $100/ft = $2,200,000
     Costs for Bacardi were not estimated because they would be
utilizing the proposed POTVI outfall.
                               VI-13

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                       VII.  LAND DISPOSAL

INTRODUCTION
     Soil, with its tremendous surface area and varied microbial
population, has been viewed as a physical , chemical ,  and biologi-
cal  filter for waste materials (98)..  The  waste is retained by
the  soil  matrix, and the organic fraction  of the waste is even-
tually decomposed by the soil  microorganisms [79,93).
     Land application is a practice whereby wastes are applied to
the  soil  surface and incorporated into the top 6 to  12 in of soil.
As in farming practices, only  the surface  soil is utilized, and
the  process of waste assimilation is aerobic.  Optimal degradation
of the added wastes requires environmental  conditions that are
similar to those needed for raising a crop: adequate  amounts of
sunlight, water, nutrients, and oxygen.   In addition, land appli-
cation and farming employ similar types  of- equipment.
     Wastes may be applied to  soils for  a  variety of  reasons.
Principal among these are the  following:
     1.  Agricultural Utilization - as a  soil amendment, utilizing
         the available organic and inorganic nutrients to enhance^
         the growth-of certain crops.
                 »•
     2.  Land Reclamation - as a soil amendment to add organic and
         inorganic nutrients to distressed or submarginal lands.
                            V11 -1

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     3.   Land Farming - as a means to dispose o'f wastes on a
         dedicated piece of land, whereby, the organic consti-
         tuents are degraded by natural  processes (oxidation and
         reduction via sunlight) and the inorganics are trapped
         within the surface soil matrix.
     The objective of this investigation is to explore the con-
cept of  land disposal of mostos and to discuss in detail, that
particular method of land application which is technologically
and economically most feasible.  As will become apparent in the
following pages, land farming for all three distillers studied
and agricultural utilization for Puerto  Rico Distillers, were
selected to offer the most feasible land disposal options.  Agri-
cultural utilization for Bacardi and VIRIL and land reclamation
for all  three were excluded from more serious considerations for
the following reasons:
     •  Agricultural utilization - from  the literature reviewed,
        research relating the effects of mostos on crop growth
        could not be found.  However, two other rum distilling
        facilities, Serral 1 es .in'.Mercedi ta ,  Puerto Rico and
        Bacardi in Tultitlan, Mexico  have successfully disposed
        of mostos by distribution (after dilution with fresh irri-
        gation water) through canals  irrigating nearby sugar-  •  >.
        cane fields.  In this instance the feasibility of dis-
                   /•
        tillery discharge depends upon the availability of nearby
        sugar cane fields.  Puerto Rico  Distillers is  the only dis-
        tillery in a similar situation in such...a favorable locale.
                              VI1-2

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        Agricultural  utilization of mostos to other crops cul-
        tivated in the islands is dependent on significant crop
        land availability and the adequate research expressing
       'the effects of mostos on such 'crops (affinity to absorb
        heavy metals, organics (toxic or otherwise), pesticides,
        etc....].   Currently, neither of  these  are  available.  The
        reader is  referred to the section, Estimation of Mosto
        Application Rates, for a more detailed explanation of the
        Serralles  and Bacardi, Mexico land disposal operations.
        Land Reclamation - land reclamation programs are typically
        employed in a situation where a waste generator is in
        close proximity to sub-marginal or distressed land,  e.g.,
        areas that have  been  heavily mined.  These  circumstances
        are not known to exist within any practical distance of
        three; di stil 1 eries.
LITERATURE REVIEW
     From the literature reviewed, the  only research that could
be found presenting relationships between mostos and soils was
that of Raul Perez Escolar (75-78).   A  series  of  four  articles
resulting from his studies was published in the Journal  of Agri-
culture of the University of Puerto Rico in.1966 and 1967.  Sum-
marized, they are  as  follows:                                   >
                                                                .₯£
     •  "Reclamation  of a Saline-Sodic  Soil by Use  of Molasses
                   /»
        and 0 i s t i-Tl ery Slops," (July 1966) - An application  of
        2.3 ac-in  of  mostos to a saline-sodic Faith clay of  south-
        western Puerto Rico was shown to lower both the soil con-
        ductivity  and exchangeable sodium. Further, he demonstrated
                              VII-3

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        that the soil  was reclaimed,  resulting in an excellent
        stable soil  structure (75).
     0  "Use of Molasses and Distillery Slops with Sulfur for the
        Reclamation  of a Saline-Sodic and a Sodic Soil  from Puerto
        Rico," (January 1967) - Tests and results similar to the
        above cited  references with sulfur included to  enhance
        reclamation.   Testing was expanded to include both a
        Guanic clay  and a Faith clay  (78).
     •  "Stability of  Soil  Aggregates Treated with Distillery
        Slops or Blackstrap Molasses,"  (July 1966) - Mostos,
        applied at a  minimum of 1/4 ac-in, was shown to produce
        a long lasting and  favorable  effect on soil aggregate
        stabilization  which persisted after the growth  of four
      "  consecutive  crops (77).
     •  "Separation  and Characterization of the Active  Soil-
        Aggregating  Agent Present in  Distillery Slops," (October
        1967) - The  soil aggregating  and stabilizing agents were
        found to be  present in the 80 percent alcohol  insoluble
        fraction of  mostos  with a composition of a mannose-
        bearing polysaccharide (6 percent), protein (7  percent),
        and a caramel  (87 ' percent) (76).
     References to mostos decomposition mechanisms and  kinetics ''
were not avai 1 abl e/rom the materials reviewed.  Further, referen-
ces to land disposal  systems could not  be found in this litera-
ture.   However, several personal  communications(33, 103, 104)
revealed the following land application programs:

                              VII-4

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Serralles - Mercedita, Puerto Rico
The Serralle's rum distillery is located in southwestern
Puerto Rico in Mercedita, several miles east of Ponce.
It is situated on approximately 4,000 acres, all of which
is owned by the Serralles family, except for some acreage
on which a sugar mill is located.  Annual production of
rum is approximately 4 million proof gallons.   Estimated
mostos flow is approximately 100,000-150,000 gal/day.
Since 1935, mostos has been discharged through  a sys.tem
of canals which supply irrigation water to about 400 acres
of  sugarcane. "The mostos is combined with other plant
waste's, achieving final dilutions ranging from  1/2 [other
plant wastes/mostos) to 5/1 (104).   This  combined  stream
is pumped to  a central irrigation station which further
dilutes the wastes prior to final field discharge..  The
combined plant wastes at this point are diluted approxi-
mately twofold.  Irrigation water is supplied by an adja-
cent river originating in the mountains north of the plant.
In 1975, a system of peripheral ditches was constructed
to prevent mostos runoff during periods of heavy rain.
Soil and groundwater monitoring programs have not been
established at those sites although, no deleterious effects
have been reported for either the soil or sugarcane (103,
104).     •-
Bacardi, Tultitlan, Mexico - Bacardi operates a rum dis-
tillery in Tultitlan, Mexico (near Mexico City).  Five

                      VII-5

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        hundred acres of sugarcane  are located approximate!y one
        mile from the plant.  There is no sugar mill present on
        the site, which would provide processing water for further
        mostos dilution.  The mosto stream is combined with other
        plant wastes achieving a dilution of 5/1 (.other plant
        wastes/mostos).  The combined solution is mixed with fresh
        irrigation water further diluting the mostos resulting
        in a final concentration ranging from 11/1  - 19/1. The
        mixture, in turn, is distributed throughout the fields
        (via canals and ditches) simultaneously supplying water to
        the sugarcane.-  crop and disposing of the mostos.  This pro-
        cess has reportedly been in operation for 17 yr,  the only
        apparent problems being occasional  ponding, resulting in
        some foul odors.  The native soil is alkaline and the
        acidic nature of mostos is reported to aid  in maintaining
        near neutral soil conditions (33).
     •  Other Mexican Operations - Other distilleries in  Mexico
        reportedly discharge their rum wastes in a  similar manner
        but are able to achieve much greater dilutions as a result
        of added sugar mill  processing water.  Mostos dilutions
        at these operations  are reported to range from 100-500
        to 1 .
COMPONENTS OF A LAN.D DISPOSAL SYSTEM
                 ,*
     The implementation of a successful  land disposal program is
dependent upon the interrelationships  of many variables  including
the selection of a disposal  site,  the nature of the wastes being
                              VII-6

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disposed,  method of waste application, and economics.   More speci
fically, these considerations involve the following parameters:
     •  Selection of Disposal Site
        -  Land area required
        -  Land avai1abi11ty and cost
        -  Proximity to distilleries
        -  Proximity to homes and commercial  establishments
        -  Ready access from all-weather roads
        -•Climate (rainfall, freedom from flooding, prevailing
          wind direction)
        -  Field slopes and general topography
        -  Hydrogeology (location of groundwater)
        -  Soil characteristics
        -  Types of crops  historically planted
        -  Ultimate land  use of disposal site.
     t  Waste Characteristics
        -  Quantities of  waste available
        -  Final waste disposal form [liquid,  concentrate)
        -  Physical, chemical, biological  properties
        -  Variability of  constituents.
     •  Application of Wastes
        -  Methods of transportation (irrigation pipe,  tanker,
          rail car,- barge . .)
                 /*
        -  Methods^of application (spreading,  spraying,  ridge and
          furrow irrigation, subsurface injection, etc...)
        -  Hydraulic loading limitations
                              V11 - 7

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        - Metals loading, pH, nutrient constraints
        - Existing local and federal regulations
        - Monitoring requirements.
     •  Economics
        - Capital costs (land,  equipment, installation...)
        - Operation and maintenance costs
        - Overall disposal  costs (total and factored).
SITE DESCRIPTIONS
Puerto Rico
     Puerto Rico has an area of approximately 3,435 sq mi.  The
island has three principal  physiographic divisions.  The most
extensive consists of the complex mountain ranges, which include
nearly all of the interior  of the island.  The less extensive are
the playa1. pi ains and the coastal plains, which circle the outer
edge of the island in a ribbonlike  band.  The elevation ranges
from sea level  to 4,398 ft  above, and the relief ranges from level
to precipitous.   Ecologically,  the  complex mountain ranges are
of volcanic origin, and the coastal  plains and playa plains are
sedimentary.   The principal rocks include several  types of Ter-
tiary and Cretaceous limestones shales, volcanic ash, lava, granite,
andesite, sandstone, serpentine, tuff, and conglomerate (86).  There
are 358 soil  types, phases, and miscellaneous land types recognized
on the island,  representing a total  of 115 soil  series  (86).
     The populatio'n dens i ties, wh i ch  vary greatly from place to
place, create the most serious  social  and economic problems con-
fronting thegovernment.  A high proporation  of  the island area con-
sists of shallow, stony, steep, or  submarginal  land (86).
                              VII-8

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     The present population is estimated to be approximately 3.4
million (1976-3.2 million).  Projections to the year 2000, suggest
a population as high as 5 million (46).  Available land, therefore,
is anticipated to be of premium value and reserved for the growth
of crops critical to the welfare of the people and the government.
     The climate is nearly ideal for a 12-month growing season.
It is tropical, uniform, and oceanic, and nearly ideal  conditions
exist for a heavy precipitation over all but the southwestern and
the extreme northwestern parts of the island,  The average annual
precipitation ranges from less than 30 in in the southwestern
part to nearly 200 in on the high peaks in the Sierra de Luquillo.
Most of the cultivated crops are irrigated in districts receiving
lass than 45 in of mean annual rainfall, and sugarcane  and grape-
fruit are irrigated in districts receiving less than 60 in.  The
climate is nearly ideal for the production of such crops as coffee,
sugarcane, pineapples, bananas, mangoes, coconuts, yautias, and
yuca, but it is poor for alfalfa and berries.  Such crops as wheat,
oats, barley, apples, pears, and peaches are not grown.  Rice is
well adapted to the climate and to many of the soils, and will
probably be cultivated to a much greater extent in the future (86).
     Rainfall in the San Juan and Arecibo areas has averaged about
60 in/yr, evaporation, about 82 in/yr.                          >..
Bacardi--        ^*
                 ,»
     The Bacardi Corporation is located approximately 1 1/2 mi
northwest of Catano.  It is bounded on the north by Ensenada de
Boca   Vieja and the village of Palo Seco; on the east by San
                              VII-9

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Juan Bay, and on the immediate west by the Palo Seco Thermoelec-
tric Plant.  Further west is the small village of Levitown.  To
the south are some marsh lands reserved for industrial  park devel-
opment and town expansions(5) .  Therefore, the only direction  ,
available for Bacardi to land dispose their wastes is west.  Some
acreage once used for sugarcane- production is approximately 4-5
mi west of the plant, but information as to the amount  of land,
ownership, cost, future planned use, etc., was not available from
the sources contacted.
Puerto Rico Distillers--
     Puerto Rico Distillers  is located on the western edge of
Arecibo.   The plant is  bounded on  the north by the Atlantic Ocean,
and on the east and south by the encroaching  fringes of the city.
Sugarcane  fields, owned by  the government are located  approxi-
mately 10 mi west of the plant.  The planned  use, ownership, etc.,
of land between the sugarcane  fields and the plant was not avail-
able from the sources contacted.
St. Croix, U.S. Virgin  Islands
     The  U.S. Virgin Islands include the islands  of St. Thomas,
St. John  and St. Croix.  The last  is the largest  of the three with
an area of 84 sq mi. "
     The  topography is  somewhat different from the other two with
a broad expanse of -low, relatively flat land  running along the
                 ,*                                  ,
 south  2/3  of  the  island.    A range of hills, ranging in eleva-
ation from about 500 ft to more than 1,000 ft, topped by Mount
Eagle at  1,165 ft, runs along the  northern coast.  In the eastern
                             VII-10

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end of St. Croix is found another group of slightly lower hills
with a maximum elevation of about 860 ft.  The relatively small
area covered by hills on St. Croix results in rather steep slopes
down to the Carribbean in the north and to the level areas to  the
south.  St. Croix is the only U.S. Virgin Island  with any sizable
expanse of flatland suitable for farming.  Here, sugarcane, which
was the principal crop, has been abandoned (84).  Subsistence
crops are now a minor effort.  Some cattle are raised for milk
and meat (84).
     In St. Croix, industrial growth has become a significant  fac-
tor in the island's economy (84).  With the downgrading of agricul-
ture,, industrial complexes have been expanded to include  the petro-
chemical industry and refinement o-f aluminum.
     One. of the principal causes of concern in the U.S. Virgin
Islands is the short supply of water.  Rainfall, while averaging
about 40 in annually (rainfall has been well  below average over
the past several years) over most of the area, is insufficient.
This is due partially to a high evaporation rate (average  about
73 in/yr) and rapid runoff.
     In an effort to utilize available water efficiently, most
homes and business establishments catch rainwater on the  roofs
and pipe it to cisterns.  Generally, during the drier portion  of^
the year, it is necessary to carry water by barge from Puerto
                  *•
Rico.
Virgin Island Rum Industries, Limited (VIRIL)--
     The VIRIL plant is located about 5 mi east of Frsderiksted,
St. Croix.  Unlike Bacardi and Puerto Rico Distillers, VIRIL is
                             VII-11

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situated in an area which is just beginning to show signs of
development.  The 25-ac plant is in a rural area surrounded by
400 to 500 ac of uncultivated land (not owned by VIRIL).  Some
evidence of urban expansion, however, can be seen. To the south
and east about 1/2 mi away, several small housing developments
have recently been constructed, and .to the north, approximately
1/2 mile, ground has been dedicated to the construction of a  hos-
pital.  The area south of the plan extending to the Carribbean
(approximately 1 mi) is not inhabited and is covered with brush,
trees, and natural grasses.
WASTE CHARACTERISTICS
     In the processing of rum, wastewater streams are generated
from four or five sources.  Mostos has been previously identified
as the stream representing approximately 66 percent of the flow,.
and 98' percent of the pollutants(93) .  For the sake of the follow-
ing presentation of data, it is assumed that only the mostos  stream
will be land disposed.
     Table  VII-1  presents  the chemical character!sties of mostos
but on a dry weight basis.  It should be noted that approximately
69 percent of the dry solids are in the form of dissolved vola-
tiles- namely organics.  While the exact nature of these are
uncertain, they are most likely a mixture of sugars, organic  acids,
amino acids, protei,ns, polysaccharides, and various organic salts.
All are decomposable but the kinetics and degradation times are
not known.  Total Kjeldahl nitrogen is 1.36 percent (dry wt basis),
indicating  that  suff i cient ..nutr i ents  are  probably  present  to

                             • VII-12

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TABLE VI.I-1.  COMPOSITION OF SELECTED CONSTITUENTS
          IN MOSTOS ON A DRY-WEIGHT BASIS.
Parameter

Total solids
Total dissolved
volatile solids
Total Kjeldahl nitrogen
Cal cium
Potassium
Chloride
Sulfate
Zinc
Cadmium
Lead
Copper
PH
Previously
Reported*
8.35
5,79'
0.114
0.209
0.426
0.211
0.412
9.89
0.18
1.10
32.8
4.36
Expressed on Dry-
Wei'ght BTa's'i's'
I

69.3
1.36
2.50
5.10
2.53
4.93
Tig/£
118
2,16
13.2
393

                        V 11 -1 3

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sustain a reasonable level of " bacteriological  activity.
     Heavy metals are in  the ranges  typically  reported for munici-
pal  sewage sludge - another waste  often  disposed to land.  On
the other hand, calcium,  potassium,  chlorides  and sulfates are
extremely high and would  probably  have  adverse effects on germi-
nation of seeds and subsequent  growth  of certain crops.
     Table VII-2  presents estimated  annual  quantities of mostos
generated by each distillery
ESTIMATION OF MOSTOS APPLICATION  RATES
Agricultural Utilization
     As previously mentioned, both Bacardi  (Tultitlan, Mexico)
and Serralles (Mercedita,  Puerto  Rico)  have active and long-
termed agricultural utilization programs which involve irrigation
canal distribution of mosto through  sugarcane  fields.  Both of
these plants have one thing in  common  - they have sugarcane fields
         TABLE VII-2.  ANNUAL QUANTITIES OF MOSTOS  GENERATED BY
             BACARDI, PUERTO RICO DISTILLERS, AND  VIRIL
Average Daily
Discharge
Distiller (gals)
Bacardi Corp. 300,000
Puerto Rico 200,000
Distillers
VIRIL 100,000
Total
Annual Total Annual
Average Number Generation Dry Solids
of Operating of Mostos Generated*
Days (gals) . (Tons)
300 90,000,000 33,066
200 40,000,000 14,696
200 20,000,000 7,346
150,000,000 55,110
 *Based on 8.35 percent solids
                               VII-14

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in close proximity to their distilleries.  Of the three distiller-
ies to which this study is addressed, only Puerto Rico Distillers
is located in such an area.  It is reported that approximately
4,000 or more acres of government-owned sugarcane fields are within
a five-mi radius of Puerto Rico Distillers (66,  103,  104).  . If
arrangements could be made with the- owner-operators of these
fields, it may fae possible to transport (via. pipeline) the entire
plant waste stream to these fields where, upon further dilution
with fresh water, the combined streams could be used for irrigation
of the sugarcane.
     From existing data on both the Bacardi (.Tultitlan) and
Serralles disposal operations, estimates of mosto application rates
(dry weight basis) and irrigation rates were made (33,  103, 104).
These are presented in Table. VII-3. Assuming that mostos is equally
distributed over the available acreage, the mosto application rate
is equivalent to 20.2 and 28.7 dry T/ac/yr at Bacardi and  Serralle's
respectively.  Similarly, from the information available,  annual
irrigation application rates of the diluted mosto streams  were
calculated to be 30.7 ac-in for Bacardi and 10.2 ac-in for Serralles.
The amount of irrigation water supplied by natural rainfall is
approximately 30 inches at Serralles, but was not available for
Bacardi.
     It would seem, therefore, that Puerto Rico Distillers, with •'
                   ,+
approximately 4,00*0 acres of sugarcane in reasonably close proxi-
mity may have a favorable disposal alternative not available to
the others.  Further, Puerto Rico Distillers' annual mostos dis-
charge is estimated to be•approximate!y 40,000,000 gals -  about
                             V11 -1 5

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i
_j
en
                      TABLE VII-3.   ESTIMATED ANNUAL  MOSTOS  AND  IRRIGATION WATER APPLICATION  RATES FOR
                             BACARDI (TULTITLAN, MEXICO) AND SERRALLES (MERCEDITA, PUERTO RICO)
Plant
Bacardi
Serralles
'i
Estimated Annual Mostos
Production Estimated Fresh Water:
(million gal) Muslos Ratio
27.5 IS:>
31.3 3.5:1
Sugar Cane
Acres
500
400
Estimated Annual Mustos
Loading Rate!
(T/ac) (dry ueiglit basis)
20.2
20.7
Estimated Annual Irrigation
Water Supplied to Dispose
of Hostos (ac-ln/yr)
30.7
10.2
              Based on 250 operating days/yr.
              Assuming inostos with specific gravity of 1.06 (8.B Ib/yal).

-------
20 to 30 percent higher than Serralles or Bacardi, Tultitlan.
The greater amount of sugarcane acreage available to Puerto Rico
Distillers suggests that tnostos could be discharged to the fields
on an even lower dry weight basis than is currently estimated for
Serralles and Bacardi, Tultitlan.
LAND FARMING  "
     There are two principal considerations which will determine
the maximum waste application rate for a land farming operation:
     t  Quantity of heavy metals in the mostos applied
     •  Hydraulic capa.city of the site (the ability of the soils
        to absorb moisture in addition to the annual precipitation)
     A third consideration, land requirements, will determine the
practicality and feasibility of a land farming program.
Heavy Metals--.
     While, there are no criteria governing the application of mos-
tos to soil, the presence of heavy metals suggests a similarity
to municipal sewage sludge, for which there are recommended limi-
tations.  In most situations mostos will be applied to soils in a.
manner analagous to sludge.  Therefore, regulations and practices
which control the use of sludge will be applied throughout this
analysis.   Table  VII-4  presents  the  U.S.  Department  of Agriculture
(USOA) recommendations for the total amounts of four metals con-;i
                                                                i'&
tained in sludge, wjiich may be added to agricultural soils (54)."
The calculations -in column four were derived from the following
formula :
„„<-*„, rriv.u r/*~\ -  Net, max allowable metal loading (Ib/ac)
mostos (dry T/ac) -  ppm meta1 in mostos (dry wt) x 0.002	
                             VII-17

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             TABLE  VI1-4.  ESTIMATION OF MAXIMUM ALLOWABLE HEAVY METALS AND SLUDGE LOADINGS  FOR  AGRICULTURE
                                        CROPS AND MOSTOS  LOADINGS FOR LAND FARMING
 I

'oo




Metal


Lead
Zinc
Copper
Cadmium


USDA Maximum
Allowable
Metal Loading*
i

2,000
1,000
500
20

Estimated
Amount of
Metal in Soil
at the Site

Ibs/ac
25
100
20
1
Maximum Allowable
Metal Loading
for
Agricultural
Soils


1,975
900
480
19
Maximum Allowable
Mostos Loading
for
Agricultural
Soils


74,811
3,814
611
4,318
Maximum Allowable
Mostos Loading
for
Land Farmingt

Tons/ac
374,055
19,070
3,055
21,590
*Total  amount  of waste metals allowed on agricultural  land for soils with CEC>15 meg/1OOg  (54).

(Based  on preliminary EPA estimate that approximately 5 times the agricultural soil metal
 accumulation  would  be the maximum acceptable loading for non-agricultural soils.

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The calculation assumes that the concentrations of  these metals
(refer to Table VII-1) will remain constant over  the  life  of  the
disposal  site.  As shown in Table VII-4, the maximum  allowable
mostos loadings vary considerably with respect to the  individual
metals, with copper being  the. limiting consti tutent.
     For land farming purposes, no recommended criteria are avail-
able.  Conversations with  EPA personnel  indicate  that  loading
rates as high as 5 times USDA criteria for agricultural soils may
be acceptable for non-agricultural soils if the metals applied
ar'e demonstrated, to be retained in the plow zone.   If  a landfarm
site will not be utilized  for agricultural purposes at any future
time, the estimated maximum allowable waste loading in column
6 of Table VII-4 may be applicable.  In this case, the'heavy metal
concentration of the. waste is not a  significant constraining  fac-
tor in determining the annual application rate to the.  site if good
soil management is practiced.  Soil  pH is of specific  concern since
.mostos is particularly acidic (pH -  4-.4).  'In order to minimize
the potential for metal.migration under  acidic soil conditions,
mostos may have to be neutralized to maintain a soil  pH of 6.5  -
7.5.  However, the. accumulation of metals in the  soil  will limit
the site life.  The life span of the site with respect to  each
metal can be calculated as follows:
     i ,- f- /1/r\ _ Maximum waste loading (T/ac)
          ^J  ' ~ Annual application  rate (T/ac/yr)
     Table VII-5 presents  these data. For mostos, the  site life
would be.limited by copper accumulation, although a site life of

                             VII-19

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      TABLE VII-5.  ESTIMATED SITE LIFE BASED ON ANNUAL
                APPLICATION RATE OF  12 AC-IN/YR*
Element
Lead
Zinc
Copper
Cadmium
Al 1 owabl e
Agricul tural
Site
625
32
5
36
Years of Disposal
Non-Agricul tural
Site
3,125
160
25
180
*Mostos assumed to have a specific gravity of 1.06 with a total
solids content of 8.35 percent.   Annual  application of mostos
equivalent to 120 dry Tons/acre.
25 yr is not a significant constraint.  Note that the calculation
is based on the assumptions that the annual application rate
remains the same (12 ac-in/yr at 8.35 percent total  solids) and
that there is no change in metal concentrations in the waste gener-
ated.  An annual application  rate of 12 ac-in/yr was selected
because it represents an equivalent loading of 120 dry Tons/ac.
For comparisons sake, a recently completed study of municipal
sewage sludge agricultural utilization programs at 9 sites showed
a range of annual sludge application rates of 15.3-146 dry Tons/ac
The median value was_ 50 dry Tons/ac (63).
                              VII-20

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     The sodium adsorption ratio (SAR) i-s also an important con-
sideration in the application of a liquid waste to soils.  Cal-
culation of SAR can be made by the following equation:

                     SAR
                          /Id
                              Z
     Wastewaters with SAR values greater than 15 should be avoided
because of their detrimental  effect on soil  structure and ultimate
reduction in the- infiltration rate of the soils.  Sodium adsorp-
tion ratio values from 5 to 15 can, over a period of years, lead
to loss  of structure  in  soil  horizons  containing  more  than  10  or
2.0 percent clay (loam or finer texture).  Lower values are gener-
ally satisfactory, although long term declines in infiltration
and percolation capacities have  been observed in moderately fine-
textured soils when irrigated with water having SAR ratios as Tow
as 3 ( 54} .
     The.  SAR  value  calculated for mostos is  1.74, suggesting that
long range application to  soils will  not  result  in degradation  of
its structure.   Further, and of more  practical  significance,  is
that both  Bacardi  in  Tultitlan, Mexico and  Serralles  in Mercedita,
Puerto  Rico,  have  practiced a "combined"  form of agricultural  ;
utilization/land  farming over a time  frame  considerably longer
than calculations  show to  be feasible, yet  neither has  reported
any deleterious  effects  to soils or crops.
                  /•
                  i*
Hydraulic  Loading--
     The  ability  of a soil to assimilate  a  specific quantity  of
waste  is  a  function of many variables,  including:
                              VII-21

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     •  Application rates       "  •
     •  Waste characteristics
     •  Soil type
     t  Location of water table
     •  Rainfall
     •  Evaporation-
     Twelve (12) ac-in is a suggested annual application rate-.
The exact. mostos application rate suitable to each location would
have to be determined in a pilot test program.  Application rates
on St. Croix, for example, may be several times greater than in
Puerto Rico because of the lower precipitation and higher evapora-
tion rates.  Mostos could be applied on a regular basis (1  ac-in/mo)
or an equivalent schedule to cope with weather patterns.  Addi-
tionally, storage facilities would have to be constructed for reten-
tion during periods of inclement weather.
     The soils of the coastal plains in and around San Juan and
Arecibo range from poorly to well-drained.  In some areas,  perched
ground waters may reach the surface during the wettest portions of
the year.  On slightly higher ground,  improved drainage characteris--
tics would be expected.  Evaporation exceeds rainfall  throughout
these coastal areas by about 22 in.
     St. Croix, on the other hand, is semi-arid and has suffered
under drought conditions for the past few years.   The  soils sur- .-.
                   fm
rounding the VIRIL plant are mainly composed of the Diamond ser-
                  <+
ies (soil which is nearly level to moderately sloping, well drained
and shallow over semi-consolidated limestone).  The subsoil is
                              VII-

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about 15 percent limestone fragments.  Hard limestone is at a
depth of about 14 in.    Evaporation exceeds rainfall by about 40
to 45 in (.84) .
     The last four factors are site specific and while detailed
examination of each is not within the scope of this preliminary
study, certain general comments are applicable.
Land Requirements--
     Table  VII-6 presents the relationship between minimum land
requirements and application rates for each of the three dis-
ti11eries.
     Land  requirements are also directly  affected  by the solids
content of mostos, i.e., as the solids content  increases, the  land
requirement decreases.  No attempt was made to correlate these,
however, since  the only practical method  of increasing solids
is  by concentration through  evaporation,  implying  a twofold treat-
ment scheme.
     In reality,  the  estimates shown  in Table  VI.I-6 must be increased
somewhat to cover contingencies such  as extended periods of soil
saturation, equipment storage, access roads, protective runoff
ditches, etc....  These considerations will be elaborated  in  the
Conceptual Design section of this report.
     Bacardi--From the data  presented in  Table 6 and expanded to'.
                                                                •'*>
account for the  abov-e mentioned factors,  Bacardi would require
                  /•
220  to  317 ac to  implement a land farming  program.  The lower
figure  would  correspond to an annual  application rate of 18 ac-in;
the  higher acreage, to 12 ac-in.
                              VII-23

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      TABLE  VII-6.  MINIMUM  ESTIMATED  LAND  REQUIREMENTS  FOR
          BACARDI,  PUERTO RICO  DISTILLERS  AND  VIRIL  AS  A
                   FUNCTION  OF  APPLICATION  RATES
Land
Plant

Bacardi
Puerto Rico
Distil 1 ers
VIRIL
Required for Annual
9 ac-in
(acres)
370
165
80
*
Application Rates of :
12 ac-in

275
125
60
18 ac-in

18.5
83
40
 *Based  on  assumption  that  heavy  metal  content  and  volume  produced
 remain  constant.
     Puerto Rico Distil1ers--Similarly, Puerto Rico Distillers
would require 121 to  155 ac for  the same application rate  (12 ac-
 in/yr).
     VIRIL--Land requirements for VIRIL are 52-80 ac.
 CONCEPTUAL  DESIGN OF  A LAND FARMING PROGRAM
 General
     Land  farming is  a viable and economically feasible option  for
 the disposal of many  and varied  waste  forms.  The following  analysis
 presents a  conceptual design of  a landfarming program-for  each  of
 the three  distillers.  Specific  site information, operating  data,
 economics,  etc., are_  unknown.  Therefore, certain engineering
 assumptions have b-een made and are documented throughout.
     State agencies  generally  prefer  land  farming sites to have a
maximum slope  of five percent.   The  minimum desirable depth to

                             VII-24

-------
groundwater is typically considered to be 15 ft, although this cri-
terion may be modified depending on the quality and uses of ground-
water and the types of overlying soils.  It is also preferred that
the soils be. deep - greater than 4- ft - and moderately oermeable.
Waste and site characteristics  assumed for  the conceptual design
are summarized in Table VII-7.  The  developmental  design  is  based  on
land farming of mostos.
     To  provide cost comparisons for differing quantities of mos-
tos disposal, the conceptual design considers three annual dis-
posal rates:  33,100 dry Tons  (Bacardi); 14,700 dry Tons (Puerto
Rico Distillers); and 7,350 dry Tons (VIRIL).  While  these figures
are only estimates, the conceptual design and resultant costs
clearly  show the economies of  scale for tile three  rates con-
sidered.
     A  sketch of the hypothetical  land farming site is shown in
Figure  VII-1,  The  topography  gf the  site  i.s, such  that slopes  range
from one to three percent, with an average's1 ope of approximately
two percent.  The site is  part of an open drainage system, which
is typical  of most humid regions.  As a result, the movement of
sediments and soluble materials from the site to neighboring water
courses  is possible.  However, the site can be modified to control
runoff.                                                         •'£.
                    f*
     Soils at the Jrypothetical  site are medium textured, such as
silt loam, with a clay content  ranging from 10 to  25  percent and
varying  with depth.  The  soils  are moderately well drained and
have an  available moisture  holding capacity of approximataly 15

                              VII-25

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                        TABLE VII-7.    BASIS  FOR  CONCEPTUAL DESIGN
         Waste characteristics:

              t  Chemical  and  physical  characteristics as shown in Table 1
              •  Annual  generation  rates  as  shown  in  Table 2.

         Site characteristics:

              •  blppe from  1  to  3  percent,  averaging 2 percent

              •  Silty loam  soil  at  least 4  ft  deep

              •  Soils are moderately well drained, with a moisture holding capacity
                 of approximately 15 percent

              •  Average precipitation  of approximately 60 in/yr in Puerto Rico,
                 and 40 in/yr  in  St. Croix

<             •  At least  15 ft  to  groundwater.
i—i
i^j        Application rate:
en
              •  12 ac-in  (120 dry  tons/ac)  per  year.

         Si te moni toring:

              •  Six water samples  taken  quarterly

                 -  Two surface water samples
                 -  Four groundwater  samples

              •  Two soil  samples taken quarterly

                 -  One surface 0- to 12-in depth or within plow layer
                 -  One subsurface 12- to  24-in  depth

              •  Ten water quality  parameters measured in each sample.

-------

ro
                   Figure;. V.I I -1.   Artist's  conception of land cultivation  site

-------
percent.   The site is assumed to have been cleared and used for
pasture or low level  agriculture for several  years.
        Land area required depends on the mostos application rate
which normally must be determined on a case-by-case basis.  The
assumed mostos characteristics are such that  an annual applica-
tion rate of 120 dry Tons/ac is appropriate for a useful  site life
of  25 years.  At  this application rate,  the productivity  of  the
site for  agricultural crops  after 25 years of  land farming may not
be  irreversibly  impaired.   It  is  preferable, however, that the
site be used  solely  for disposal  purposes; no  crop is grown  dur-
ing  or after  completion of  disposal operations  to  prevent  intro-
duction of  toxic  substances  into  the food chain.   Further, from
what has  been presented earlier,  the actual site life may  be con-
siderably larger  than that  calculated as evidenced by the Serrales
and  Bacardi,  Mexico  land  disposal operations.
     A surface and groundwater monitoring program  is  a necessary
part of any  land  farming  design.  To adequately monitor surface
water near  or adjacent to a  site, two samples  should  be taken
at  quarterly  intervals.   One sample point should be located
upstream  from the  site and  the other downstream.
     The  number  of wells  required to adequately monitor ground-
water at  each site depends  on  the complexity of the subsurface -'
                                                                ••i
hydrology.   For  each  hypothetical site, four wells are specified.
Two  wells are locfated at  the upstream boundary  of  the sits to
establish background  water  quality.  Two wells  are located down-
stream from  each  site to  establish the distribution and impact

                             VII-28

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of localized contamination (if any) on water quality in the aqui-
fer.   More wells would be desirable, but cost considerations will
usually indicate that a plan be developed to ensure most effec-
tive coverage with the least number of wells.
     The suggested soil monitoring program entails taking 10
soil  samples each at the Q to 12 in and 12 to 24 in depths
prior to the first mostos application and at quarterly intervals
thereafter.  The samples at each depth are composited, processed,
and anlyzed.  Data obtained for the soils which have received
mosto can be compared with the results for the controls (samples
obtained prior to first mosto application).  This comparison will
indicate any accumulation and the extent of vertical migration of
most constituents (e.g., heavy metals) beyond the plow layer.  It
is recommended that the following parameters be monitored'-
     r  Total dissolved solids (TDS) or soluble salts (EC)
     •  TOC
     •  pH
     •  Sulfates
     •  Chlorides
     t  Nitrate-nitrogen
     •  Iron
     •  Copper
     •  Potassium
                   /-
     •  Cadmium
     Other parameters of concern  such  as  selenium,  boron,  and
toxic organics should be included in the  water  and  soil  mor.itor-

                              VII-29

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ing programs if these constituents are present in significant
concentrations in the incoming mostos.
     All analytical determinations can be performed  by a  contract
laboratory.
     Quarterly water sampling should  be adequate for monitoring
site performance.  Such a sampling frequency has been typically
employed by numerous land farming site operators and state regu-
latory agencies.
Site Design
     For the purposes  of the following discussion,  it is  assumed
that the site selection process has been completed.   Since each
site's  topography is  gently sloping  and relatively  flat,  substan-
tial grading is not required to  facilitate land farming  operations.
The site has been used previously for pasture or low level agri-
culture, so substantial  clearing is  unnecessary. Uncontrolled
sheet runoff from the site's surface  during  periods  of intensive
rainfall could result in contamination of adjacent  surface waters.
For this reason, construction of a system of berms  and runoff
collection ditches around the site perimeter prior  to initiating
land farming activities fs specified.  This  surface  drainage sys-
tem will eliminate standing water in  the cultivated  area, thereby
ensuring that aerobic processes  necessary for mosto  decomposition
are maintained.    '
     Appropriate liming of the soil  or neutralization of mosto may
be  necessary to maintain a soil  pH between 6.5 and  7.5.   Addition-
ally, application of nitrogen fertilizer may be needed if the
waste is highly carbonaceous (carbon  to nitrogen ratio <30) to
                              VII-30

-------
enhance decomposition.  These parameters will have to be inves-
tigated further in a pilot plant program.
     An access control fence is placed outside the disposal area's
ditch/berm and within 100'ft of the site perimeter.  A 30  ft gate
is located where the access road enters the site.
     The site includes a paved access road to an office/equipment
storage building.   The building is prefabricated aluminum  on a
cement slab, with plumbing and utility connections.  A 10  x 20
ft office extension are-a is furnished for the a.ttandant and includes
restroom facilities.  The remainder of this structure' houses the
cultivation equipment and is closed to the weather on three sides.
Mostos Application Procedures
    .Selection of an application system depends upon several fac-
tors including facilities at the distillery, transportation costs,
required flexibility, capacity and regulatory agency requirements.
In most instances, regulatory agencies require that wastes be
covered or mixed with soil soon after application to prevent
aesthetic problems and contamination of runoff waters.
     Subsurface injection has proven successful in many situations.
These systems are normally used with liquid's in the range  of 1 to
10 percent solids.  Although subsurface injectors particularly  •
the continuously operating types, can apply liquid wastes  at rela-
                                                                J?,>
tively high rates, factors such as transportation methods, costs
                  ^»
and storage faci1fties for inclement weather require serious con-
sideration.
     Selection of a truck- or tractor-mounted injector unit depends,
to a large extent, upon the goals established for the land farming
                              VII-31

-------
program, continuity of and access to the application site, and
the quantity of waste that must be applied in a given time period.
A truck-mounted unit offers the primary advantage of increased
flexibility.  That is, since waste is supplied from a mobile tank,
the unit is essentially self-contained.  It can be used both to
transport and apply waste and can easily be used to service several
sites.  Typical productivity of truck-mounted units ranges from
30,000 to 80,000 gal/day and approximately 1,000 gal/acre per pass.
     Primary advantages of the tractor-mounted unit are high pro-
ductivity  (200,000 to 300,00 gal/day)   and high application rates
(25,000 to 50,000 gal/acre per pass).  Track-type tractors can
operate in more adverse weather and/or  soil conditions  than wheel
tractors or truck-mounted units.   Tractor-mounted units, however,
require more sophisticated controls and waste handling  facilities
to achieve maximum operating efficiency.  Waste is supplied to the
tractor-mounted injector through  a long flexible hose,  and a fixed
liquid distribution system may also be  required on the  site.
     Subsurface injectors normally use  agricultural tillage tools
(chisels or sweeps) to open cavities in the soil, allow liquid
waste to flow into the cavities,  and then cause the soil to bridge
or cover the openings.  Sweeps generally provide better covering
at shallower injection depths and provide better mixing of the
waste and soil.  Good mixing of the waste and soil at shallow
depths enhances evaporative drying, accelerates assimilation of
the waste into the soil  environment and practically eliminates
odors, insects, and runoff problems.  Further, in visual appear-

                              VII-32

-------
ance,  the operation seems to be a normal agricultural practice.
Careful  landscaping or screening of storage and other handling
facilities aids in eliminating the appearance of anything aes-
thetically offensive.
     The following detailed analysis presents two scenarios: the
first utilizes a tractor-type application vehicle fed from on
site (land farm) holding tanks.   Mosto is transported to the site
via tanker trucks.  The  second utilizes the same application
vehicle but on site mostos  is supplied via a  buried pipeline.
Via Tanker Truck--
     Mostos is transported  between the distillery and land farm-
fng site via 6,000 gal tanker trucks.  A 10-day storage tank is
provided at each distillery to provide a buffer.during  periods
of inclement wea.ther.  A further 2-day capacity storage tank is
provided at each land disposal  site to supply the application
vehicles.  Application of mostos (assumed total  solids  of 3.35
percent) is accomplished using a subsurface injector mounted on
a crawler  tractor, the discharge from which is rated at 800 gpm.
Application units are fed from the storage'tank through a dis-
tribution  system consisting of 10-in buried cast iron pipe ter-
minating  in a  hose connect.  The  hose is heavy-duty flex type, -•,.
                                                                 " •»
typically  600-700 ft  in  length and 4-in diameter
     Table  VII-8  s'hows  the  cultivation  areas,  quantities  of  mosto,
and storage capabilities required for each of the three sites
at  the  suggested 12  ac-in application rate.  It is  assumed that
the increase  in the  areas shown  (total) will be required for
                              VII-33

-------
the access  road, perimeter berm, buildings, runoff  control facili-
ties, and  a  100-ft buffer on all sides.   Figure  VII-2  presents a
representative profile  of the site,  indicating several  of the
key features previously discussed.
     Mostos  is applied  on a daily basis  except in extended periods
of heavy  precipitation  during which  the  soil will not  support  the
weight  of  the crawler tractor.  It  is  assumed that  soil applica-
tions can  be made an average of 210  days/year and that  application
will be  restricted to a one shift/day  operation.

           TABLE VII-8. CONCEPTUAL  DESIGN WASTE AND SITE PARAMETERS   •
Parameter?

Estimated annual volume (gal)
Gallons per operating field day
Bacardi
90,000,000
423,000
Puerto Rico
Distillers
40,000,000
190,000
VIRIL
20,000,000
95,000
     (assume 210 working days/yr)
     Estimated annual total solids
     disposed.(? 3.35% solids) (Tons)
                  33,066
H,S96
     Land area required
     (12 ac-in/yr):
.acres
7,343
• For mostos disposal
* Total (including area for access
road, building, ditches, buffer
zone, etc.)
Storage tank volume at distillery
with 10- day storage capacity
(mill ion gal )
Storage tank volume at land
farm sita with two-day storage
capacity (million gal].
275 125 SO
317 155 80
4.3 1.9 1.0
0.86 0.38 0.19
                               VII-34

-------
  FENCE
 TOO F
BUFFER
 ZONE

                        EQUIPMENT STORAGE
                        BUILDING AND OFFICE
                                                         FENCE
                                              LAND CULTIVATION FIELD
RUNOFF
CONTROL
DITCH fc
BERM
                                       MONITORING WELL
RUNOFF
CONTROL
DITCH t
BERM
                Figure VII-2.   Representative  site profile

-------
     Truck transport of mostos in 6,000 gal  tractors will  operate
on an extended (8 or more hours/day)  schedule, approximately 210
days/year, (more for Bacardi)  to maintain feedstock to the field.
Times to load at the distillery, travel to the site, unload, and
return are estimated to be (assuming  site is  five miles from
distillery) 10, 15, 10, and 15 minutes, respectively.   Further,
it is estimated that this operation will  be  70 percent efficient,
therefore requiring a total cycle time per tanker of 71 minutes.
     Based on the above (presented in Table  VII-9).  Bacardi will
require nine tractor trucks,  Puerto Rico  Distillers four,  and
VIRIL,  two.   Each, will  require one additional  cab  for preventive
maintenance and nonscheduled  repairs.  Similarly, at a rated
capacity of 800 gpm, Bacardi  will need two crawler-tractor applica
tion vehicles to handle their  daily disposal  volume; both  Puerto
Rico Distillers and VIRIL will require only  one each.   Labor
requirements basically will follow the manning of equipment pieces
as shown in Table VII-10.
                             VII-36

-------
       TABLE VII-9.   CONCEPTUAL DESIGN VEHICLE REQUIREMENTS FOR LAND  FARMING
                 UTILIZING TANKER TRUCKS FOR iMOSTOS  TRANSPORTATION
          Factors
          Puerto Rico
Bacardi    Distillers
Gal/land  farm operating  day (gal)

Tanker loads/day land farm @ 6,000 gal
  per tank

Total  cycle time for one tanker load
  @ 70* efficiency (rain)

Number of tank trucks required per day
  assuming one shift - 10 hr/day

Actual  number of tanker  vehicles
  required:
428,000

  72


  71


  8.4
190,000

  32


  71


  3.7
              TABLE VII-10..  LABOR. REQUIREMENTS  FOR LAND
                  FARMING  UTILIZING TANKER  TRUCKS  FOR
                         MOSTOS  TRANSPORTATION
            YIRIL
95,000

  16


  71


  1.9
• Tankers
• Cabs
Number of tractor-crawler field
applicators required at a.
capacity of 800 gal/min
9
10
2
4
5
1
-2
3
1


Tanker Truck Drivers
Crawler-tractor Drivers
Full Time Mechanics
Foreman
Relief Personnel >•
Total

Bacardi
9
2
2
1
1
15
Puerto Rico
Disti 1 lers
4
1
1
^
-
6

V I R I L
2
1

—
-
3
 *Assume  that employee assigned to  crawler-tractor  can
  substitute as a  part-time  mechanic.
                                   VII-37

-------
Via Pipeline--
     This scenario is similar in all respects to that presented
in the previous section with the following exception:  mostos
is supplied to the land farm site via an 8 to 10 in diameter
b.uried pipeline.
     Mostos storage is assumed to remain the same - i.e., a  ten-
day capacity tank at the distillery and a two day capacity tank
at the land farm site.  Labor requirements, revised from Table
VII-10 accordingly, are presented in Table VII-11.
            TABLE VII-11.   LABOR REQUIREMENTS FOR LAND
                  FARMING  UTILIZING A PIPELINE
                    FOR MOSTOS TRANSPORTATION


Crawler-tractor Drivers
Full Time Mechanics
Total
Estimated Costs for Concep

Bacardi
2
3
. 5
tual Land Farming
Puerto Rico
Distillers
1
2
3
Site

VIRIL
1
1
2

     Both capital  and operating  and maintenance costs are estima-
ted.
     Capital  costs associated with land farming for both scenarios
are presented in Tab-les VII-12 and VII-13.  The recovery period
                  f»»
("useful  life" or  "payback" period) varies  according to the type
of investment. All capital  costs have been  annualized based on an
11 per-cent interest rate (on internal  rate of return)  over their

                              VII-38

-------
TABLE VII-12.   CAPITAL COSTS FOR THREE CONCEPTUAL LAND FARMING SITES
        UTILIZING TANKER TRUCKS FOR MOSTOS TRANSPORTATION*
Cost Element
Debt service for
purchase of land @
Recovery
Period
(yr)

Bacardi
Total Annual
523,000
(317 ac)
Puerto Rico
Distillers
Total Annual
255,800
(155 ac)
VIRIL
Total Annual
132,000
(80 ac)
11% yr; ba'secl on
original price of
$15,000/ac

Site preparation:      20        95,100        11,900       46,500
  Leveling, some
clearing, survey-
ing, design @
$300/ac

Site work:             20       349,000        43,000      171,000
  Fencing, build-
Ings, roads, run-
off prevention
ditch & $l,100/ac

Monitoring wells:      20         4,000           500        4,000
  4 to 7 1n wells,
60 ft deep, In-
cluding pumps and
casing & $1,000/
well

Storage tanks &
$.30/gal.
  • At the distillery   20      1,290,000      162,000      570,000
  • At land farm               258,000        32,400      114,000
                                                   5,840
                                                  21,400
                                                     500
                                                  71,600
                                                  14,300
 24,000
 88,000
  4,000
300,000
 57.000
 3,010
11,000
   500
37,700
 7,160

-------
       TABLE VII-12  (continued)
o
                           Recovery
                            Period
                             (yr)

                             10
Cost Element

Field distribution
system:
  From farm storage
to field  stations;
includes  pumps,
risers, underground
pipe @ $l,000/ac
       Electrical  equipment:  20
          Includes  installa-
       tion  of power at  site,
       transformers, con-
       trols & $600/ac
       Property tax  and
       insurance  & 1.5% of
       in-place capital
       investments
       Injection  equipment;
         Includes  crawler-
       tractor, hoses,
       reels,  freight

       Communication
       equipment

          Subtotal
                       10
                                      Bacardi
 Total
                                  5,000
Annual
317,000      53,800
                                190,200      23,900
                                            35,200
240,000      40,000
              1,350
                             Puerto Rico
                             Distillers
 Total

155,000
                          93,000
                              2,748,300     928,650
            120,000




              5,000


          1,278,500
Annual

26,300
                         11,700
             16,605




             20,400




              1,350


            445,795
                                            VIRIL
Total

80,000
                          48,000
            120,000
              5,000
                                                  726,000
Annual

 13,600
                           6,030
                                                                 8,650
             20,400
              1,350
                         241,400

-------
 TABLE VI1-12  (continued)
Cost Element
Tanker trucks &
Recovery
Period
(yr)
10
Bacardi
Total Annual
580,000 98,500
Puerto Rico
Distillers
Total Annual
280,000 47,500
VIRIL
Total Annual
180,000 30,600
$60,000/unit
($40,000-cab;
$20,000-tanker)
Total Capital
Investment

Annual Capital
Costs

Annual Capital
Costs/Dry T

Annual Capital
Costs/1,000 gal
Mostos
3,328,300
            1,027,150
1,558,500
                   31.06
                   11.41
              423,295
                   33.57
                   12.33
906,000
             272,000
                  37.03
                  13.60
*"Total" amounts are initial  costs,  "annual" amounts are for first 12 months  of operation.  All costs
 are in 1978 dollars.
 Annualized capital  recovery  (equal  annual payments) at 11% interest rate.

-------
I
-F".
ro
                           TABLE VII-13.   CAPITAL  COSTS FOR  THREE CONCEPTUAL  LAND  FARMING SITES
                                        UTILIZING A PIPELINE  FOR  MOSTOS  TRANSPORTATION*
Recovery
Cost Element* Period (yr)
Subtotal from Table 12
Pipeline: 5 ml of 6" dia. 20
pipe, including acces-
sories e $ia/ft
Pump stations: 2/slte 10
@H 50.000 ea
Debt service for right 20
of way lease & $15.000/ac
(lit Interest)
Property taxes and
insurance $ 1.5% of 1n-
place capital invest-
ments
Total capital Investment
Annual capital costs
Annual capital costs/
dry T
Annual capital costs/
1,000 yal mostos
Oacardi
Total Annual
$2,74B.300 $ 920.650
343.200 43.100
300 .000 51 .000
16,500
9.650
$3.391.500
$1,040.900
$ 31.72
$ 11.65
Puerto Hi co Distillers
Total Annual
$1.270.500 $445.795
343.200 43.100
300,000 51.000
16.500
9.650
$1.921.700
$566.045
$ 30.52
$ 14.15
VIIIIL
Total Annual
$ 726,000 $241,400
343.200 43.100
200.000 51.000
16.500
9.650
$1,369.200
$361 .650
$ 49.23
$ 10.00
               '"Total" amounts are initial costs, "annual" amounts
                are for first 12 mo of operation.  All  costs are
                In 197U dollars.
               t/Wmualized capita).recovery (equal annual payments)
                at 11* interest rate.

-------
recovery period.   Major capital  cost categories are land, site
preparation and construction, and equipment.
     The estimated unit cost for land is $15,QOO/ac.  However,
land is considered an appreciating asset and as such is not con-
sidered in the same light as other investments.  Rather it is
shown as an annual cost in the form of debt service.
     Tables VII-H and VII-15 respectively present annual capi-
tal  and operating costs for both scenarios.  From the data shown,
lower total annual costs are. shown for pipeline transport for
Bacardi and Puerto Rico Distillers. From the scenarios presented,
the  less expensive option to VIRIL would appear to be tanker trans-
port.  This would change, however, if VIRIL would be able to
utilize the adjacent land surrounding the plant (.the scenario is
for  a disposal site five miles from the plant.
     Table 711-16 presents a summary of Tables  VII-12, VII-T3,
VII-H and VII-15.
     In considering the foregoing analysis, however, several
qualitative factors should be included when comparing the scenarios
These are:
     •  Social and aesthetic cost  are intangible but should be
        considered.  For example, the logistics of Bacardi hauling
        70 or more daily loads (6,000 gals each) of mostos on   -:;
                                                                -X
        reasonably tuisy roads would suggest considerable traffic"
        congestion-^ noise, and possible annoyance to roadside
        vi 11 agers.
     •  Further,   no costs have been included for highway and road
        repairs.   These are difficult,  at  best,  to  calculate,  but
                               VII-43

-------
                              I
                              I.  •
  TABLE VI1-14.   ANNUAL OPERATION AND MAINTENANCE  COSTS  FOR THREE

LAND FARMING SITES UTILIZING TANKER TRUCKS  FOR  MOSTOS  TRANSPORTATION

Cost Element
Labor @ $10/hr, Including fringe
benefits
Operation and maintenance of vehicles
@ 20% of capi'tal costs; includes fuel
and replacement parts
Monitoring (32 samples x 10 parameters
ea @ $20/parameter plus other testing)
Annual operating costs
Annual capital costs
Subtotal
Administrative costs @ 15%
Total annual costs
Annual costs/dry T
Annual costs/1,000 gal mostos

Bacardi
$ 312,000
164,000
10,000
486,000
1,027,150
1,513,150
226,970
1,740,120
52.63
19.33
Puerto Rico
Distillers
$ 124,800
80,000
10,000
214,800
493,295
708,095
106,214
814,309
55.41
20.36

VIRIL
$ 62,400
60,000
10,000
132,400
272,000
404,400
60,660
465,060
63.31
23.25

-------
TABLE VI.I-15.   ANNUAL OPERATION AND MAINTENANCE COSTS  FOR
          THREE LAND FARMING SITES UTILIZING A
           PIPELINE FOR MOSTOS TRANSPORTATION
Cost Element
Labor @ $10/hr, including fringe
benefits
Maintenance:
t of pipeline @ 2% of
capital investment
• of pump stations @ 2># each
of capital investment
Operation and maintenance:
t of crawler- tractor vehicles
@ 20% of capital investment,
including exchange parts
and fuel
Power and supplies for operation of
pump stations @ $15,QOO/yr
Monitoring (see Table 14)
Annual operating costs
Annual capital costs
Subtotal
Administrative costs @ 15%
Total annual costs
Annual cost/dry T
Annual cost/1 ,000 gal mostos
Bacardi
$ 104,000

6,860
7,500

40,000
15,000
10,000
183,360
1,048,900
1,232,260
184,839
$1,417,099
$ 42.86
$ 15.75
Puerto Rico
Distillers
$ 62,400

6,360
7,500

20,000
15,000
10,000
121,760
566,045
687,805
103,170
$790,975
$ 53.82
$ 19.77
VIRIL
$ 41 ,600

6,860
. 7,500

20,000
15,000
10,000
100,960
361 ,650
462,610
69,392
$532,002
$ 72.42
$ 26.60.
                            VI1-45

-------
                 TABLE VI1-16.  SUMMARY OF ANNUAL CAPITAL AND
                  OPERATING TOTAL COSTS FOR TANKER TRUCK VS.
                         PIPELINE MOSTOS TRANSPORTATION
         Cost Element
Tanker truck transportation:

   Annual capital costs/dry T

   Annual capital costs/1,000 gal
   mostos

   Annual operating costs/dry T

   Annual operating, costs/1,000 gal
   mostos
Bacardi
$31.06

 11.41


 52.63

 19.33
Puerto Rico
Distillers
  $33.57

   12.33


   55.41

   20.36
 VIRIL
$37.03

 13.60


 63.31

 23.25
Pipeline transportation:
Annual capital costs/dry T
Annual capital costs/1 ,000 gal
mostos
Annual operating costs/dry T
Annual operating costs/1,000 gal
mostos

31.72
11.65
42.86
15.75

38.52
14.15
53.82
19.77

49.23
18.08
72.42
26.60
                                   VII-46

-------
        could  become significant,  especially so for roads designed
        for normal  passenger car traffic - not fully loaded tanker
        trucks.
     «   Annual  capital  costs for both scenarios can be significantly
        reduced  if  the  cost of land is less than shown.  Addition-
        ally,,  long-term leases may be negotiated at a fee much
        less than that  used for debt service.
     •   No costs are presented, for appreciation of land values.
        If purchased,  this land would be expected to increase
        in value, thus  increasing  the net worth of the respective
        purchasing  companies.
     *   All estimated  costs for the conceptual designs are based
        on 1978  dollars.   It is to be expected that those costs
        will rise in the  future, du.e to inflation, but no cost
        projections were  made because of uncertainty in the infla-.
        tion rate.
     The annual  operating costs of mostos pipeline transport
(Table Til-15)  are  14  percent of -the to ta^l. annual costs.  The sane
values  for tanker truck transport  show the annual operating costs
to be 47 percent (Table VII-14). The comparison is significant since
those operating  dollars needed for annual support of a tanker
truck fleet,would be expected to increase with inflation.  Total •
                                                                 'i
operating costs  for pipeline transport  are similarly subject
                   /•
to inflation,  but'.would be expected to increase less in overall
total dollars  since they  represent a smaller overall percentage
of the  total annual costs.  The relative cost of pipeline trans-
port, therefore, would  be expected to become more economically

-------
favorable in the ensuing years, assuming that the inflation rate
continues to increase.
     As expected, the total unit costs shown indicate that larger
land farming sites are less costly to operate on a unit cost
basis than are smaller sites. For comparisons the range of unit
costs on a dry weight basis for either scenario are within the
range of municipal sewage sludge disposal costs typically repor-
ted.
     Finally, Table VII-17 presents the effect of each scenario in
terms of annual proof gallon production (1977 figures).  It was
assumed that production figures were 17.4, 5 .2 , and. '3..'4 mi 11 ion
proof gallons respectively for Bacardi, Puerto Rico Distillers,
and- VIRIL.
            TABLE VII-17.  ANNUAL  LAND  FARMING COSTS
                   OF MOSTOS AS A  FUNCTION  OF
                          RUM PRODUCTION
Scenario A - Transport
  via Tanker Truck
Scenario B - Transport
  via pipe!ine
                                        Puerto Rico
                           Bacardi      Distillers        VIRIL
                                      $/proof gal Ion
0.1000        0.1566.         0.1368
0.0814        0.1522         0.1565
                              VII-43

-------
IDENTIFICATION OF RESEARCH NEEDS
     The preceeding sections have outlined the basic fundamentals
of a land disposal program and have carried these through in the
form of a conceptual  land farm design.  Throughout the discussions,
certain key parameters were identified, which, if quantified, would
facilitate a- more detailed and precise feasibility evaluation.  A
pilot study would, therefore, seem a logical  first step to obtain
the required data.   The. following program describes the equip-
ment, site preparation, mos.tos applications,  and monitoring recom-
mended to determine the acceptability of the  landfarming prac-
tices described in the Conceptual Design section.
     A prerequisite,  however, to this en.tire  program is a thorough
exploration with local regulatory agencies for necessary permit-
ting and acceptance of the concept.
Equipment Required
     For the conduct of this small pilot program,it is recommended
that the necessary equipment.be rented, either with or without an
operator.  For  best results, the same  type of equipment which will
be  used for the full-scale land farm should be used for the pilot
test program.   This,  however, is not practical in all  cases,
expecially so with reference to subsurface injection equipment.
                                                                >
In  lieu of not  having  these specialized pieces,  it is  recommended;
                   r'
that conventional surface applications followed  by disking be sub-
                  /•
stituted.  The  results are generally comparable.  It is anticipa-
ted  that  the  following equipment will  be required:
                              VII-49

-------
     t  Farm tank trailer (1,500.- 2,000 gal capacity) equipped
        with exit valve and bar or fan spreader
     •  Tractor
     •  Roto-tiller or disk (.3-tier, 10 ft wide, 18 in diameter).
The program should be implemented on a small plot of land (10 to
15 ac) and should be allowed to run for 6 to 9 mo, or an equivalent
time frame to cover the seasonal variations.  The location of the
site is preferably near either the Bacardi or Puerto Rico Distillers
facility.   It may be assumed with a certain degree of confidence
that application rates and other parameters determined in Puerto
Rico will  be applicable to a similar operation on St.  Croix.  The
opposite,  however, may not be the case considering the current
drought conditions of St. Croix.
     The test and control plots should be constructed so that they
are of equal size and are located on identical soils.   A berm
should be  constructed around the perimeter of the test area and
between the test plots to contain runoff and mosto applied within
the fi.elds.
Mostos  Application
      In the  pilot  program, mostos  should  be  applied at rates  equi-
valent  to  6, 9,  12,  15,  and 18  ac-in/yr  to  each  of  5-two  ac  plots.
A  sixth 2-ac plot  would  serve as  a  control  (no mostos would  be  .^
added  to  this  plot 'for the duration of the  testing  period).   Appli-
cations can  be  made  by filling  the  tank  (known volume) with  mostos,
driving to  the  particular  plot  designated  for  the  specific  appli-
cation  rate, and  subsequently discharging  the  \oad  at ?.  previously

                              VII-50

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calculated speed equivalent to the prescribed application  rate.
Depending on the weather, the mostos 'will be allowed to dry for a
few days (or more if it rains), and then mixed with the surface
soils to a depth of 6 to 8 in, with a roto-tiller or tractor-
pulled disk.  Disking should be accomplished after each applica-
tion and may have to be increased if odor problems develop.
     Mostos should not be applied when the soil is excessively
wet, since the equipment may get stuck in the mud and odors may
possibly develop from casual ponding.
     To facilitate evaluation of the pilot test .program, ' precise
records need to be maintain-ed on the time, quantity and percent
solids at which each load of mostos is applied to the plots.  In'
addition, cultivation records need to be kept.
Monitoring
     Soil characteristics and hydrogeologic data for the sita
should be carefully investigated.  Of particular concern are such
factors as permeability, soil pH, and distance to groundwater.
Major environmental concerns typically, however, are soil  accumu-
lations of heavy metals and the potential for surface water con-
tamination .
     In the test program, soil samples from 0 to 1  ft, 1 to 2
ft, and 2 to 3 ft depths should be collected prior to the  first 4
mostos application.^ Subsequent sampling should be conducted at
                  i"
three-month intervals.  These samples are to be analyzed for heavy
metals (e.g., Cd, Pb, Cd, In, etc.), organic matter, total  Kjeldahl
N, pH. nitra.te-N, chlorides, and sulfates.   If these constituents
                              VII-51

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show significant increases in concentration with waste application,



the extent to which they build up in soil  and potential  for sur-



face water contamination can be assessed.



     Likewise, runoff from the plots should be collected periodi-



cally and analyzed for the above-mentioned constituents.



     Odor problems, if any, are to be noted during daily inspection



of the plot,  particularly after spreading  of the mostos  and on



wet days.



     Of special  concern is the effect of mostos on soil  pH.  As



mentioned earlier, mosto is very acidic (pH = 4.4) and may require



neutralization prior to application.  Alternately, liming  of the



soils may be  required.
                              VII-52

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                       VIII. . EVAPORATION

     Evaporation of mostos, when viewed as a treatment technology,
must be examined as a function of two considerations:
     •  Available technology
     ».  Disposal options for secondary products (concentrates
        and side waste streams).
     The technology for evaporation of cane molasses stillage
has been successfully, demonstrated (57, 58, 59, 60).  Typically,
the end result of such treatment is the production of a secondary
product known as condensed molasses solubles (CMS) with a solids
content in excess of 50 percent.
     European and United States distillers have demonstrated
that CMS can be successfully marketed.  Puerto Rico and the Virgin
Islands, however, are islands with limited potential for domestic
CMS consumption (see "Potential for CMS Use in Puerto Rico and
the(U.S. Virgin Islands" for a detailed discussion).  An in-depth
analysis of evaporation as a disposal option, therefore, will be,.
directed toward discussions of both the technologies available to
                                                                 •^
produce secondary products and the alternatives available to dis-;i
pose of these (fin
-------
hearings in the matter of NPOES permits for Puerto Rico Dis-
tillers, Schenley, and VIRIL (  15» 24, 34, 87 and 57-60). Similar
experience has also been reported by Bacardi  (  4).  This testimony
is assumed to be accurate and reliable.  Included in the testimony
are some cost estimates which are typically subjective judgements
of the party offering testimony.   The reader  should keep in mind
that these estimates are provided for general  information without
substantiation.  Additional  technological  input to the various
processes employed has been  supplied by equipment manufacturers.
Theory, Design, and Operation
     Two basic evaporation principles have successfully been
utilized by the distillery industry to treat  liquid "waste streams -
multiple effect and mechanical  recompression.   Within each cate-
gory, various evaporator types  are available  to handle evapora-
tion duties with maximum efficiency and economy.  The most com-
mon types currently in use by the distillers  are either plate
(falling or rising film) or  tubular evaporators (falling film).
The following section will briefly discuss each of the above and
their interrelationships.
     The principle of multi-effect (ME) operations  allows several
single evaporation units  (known as effects) to be connected in  !
series.  This system involves the multiple reuse of the heat con-
tent of steam.  By ,this method, the energy contained in a single
pound of steam may be used to evaporate anywhere from two to eight
pounds of water, depending on the number of effects and the aux-
iliary equipment employed.  Steam from a boiler enters the heating

                             VIII-2

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jacket in the first of a series of interconnected evaporators.
This steam boils the mostos in the first  evaporator and conden-
ses in the heating jacket after it has transferred its heat con-
tent to the mostos.  This condensate is returned to the boiler.
The water vapor from the boiling mostos is piped to the heating
jacket in the second evaporator, where it boils the mostos in
that evaporator.  This process is continued as many times as
the number of effects in the system (3).
     Mechanical vapor recompression (MVR) entails increasing the
heat content of the water vapor from the evaporation process.
The recompression is generally applied to the vapor from the first
effect in the system, causing an increase in the heat content of
the vapor and.enabling it to evaporate more water per initial
unit of heat input (3).    Some manufacturers produce MVR evapora-
tors only as single effect units (3).
     Generally, the evaporators which would be considered for
application to mostos are designed on the basis of either thin
film or forced circulation principles (3,  28).    Five basic eva-
porator designs employ the thin film principle (3):
     •  The falling film tubular evaporator
     •  The rising film tubular evaporator
     •  The falling film plate evaporator                       ^
                                                                 .-»
     i  The free flow falling film evaporator.
                 i»'
Thin film systems^are offered as single-or multiple-effect units,
with or without MVR.
                             V 111 - 3

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     Forced circulation evaporators are designed for processing
liquids which are susceptible to scaling or crystallizing (74,75).
Two basic types are offered  (3):
     •  The forced circulation tubular evaporator
     •  The forced circulation plate evaporator.
Forced circulation systems are offered as single-or multiple-
effect units, and may be combined with film evaporator pre-
concentrating effects.  MVR can be considered with forced cir-
culation systems, but economic considerations usually do not
favorsuchadesign  (28).
     The energy efficiency of an evaporation system can be
increased through the use of additional effects and MVR, but
only at the cost of increased capital  outlays from the. addition
of such equipment.  MVR is theoretically  the most energy-efficient
evaporation method (34).  MVR provides its greatest energy cost
savings in applications where the cost of electricity is low and
the operating hours per year are relatively high.
Applicability to Mostos
     Evaporation of molasses stillage  is a common occurrence in
Europe, but, with few  exceptions, it is applied to beet instead
of cane molasses stillage.  Two exceptions are the Bols Distillery
in the Netherlands and the Bacardi  rum facility in Malaga, Spain;.
Bols has a four-effect evaporator of the falling film type with
a separate finishi-ng  unit.  This unit  has been in use for four
years.  The Bacardi,  Malaga, evaporator, designed by Vogelbusch,
is a multiple-effect  evaporator with forced circulation for final
concentration. Multiple-effect  evaporators  are  currently  in  u'se  at
                              VIII-4

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the Sols Distillery in Scheidam, the Netherlands; Florida Dis-
tillers, Lake Alfred,  Florida; and Bacardi, Malaga, Spain. Prior
to plant closure, Standard Brands in Peekskill, New York, also
utilized multiple effect evaporation.
     The Sols Distillery is not specifically a rum distillery,
but it produces liqueurs from the distillation of cane molasses.
The differences between liqueurs and rum result from processing
and formulation stages after distillation.  Therefore, the com-
position of the raw waste discharge from the Sols Distillery is
similar to that from a rum distillation plant.  The equipment at
the distillery is made of type 316-1 stainless steel to permit
cleaning with both dilute hot' nitric acid, as well as with dilute
hot caustic soda. Normally, four to six hours of cleaning are
required out of a 24-hour operating period to remove organic and
inorganic scale deposits (59).
     Although Bacardi  ' s m-ul ti pi e-effect evaporator at Malaga,
Spain, has been.in actual operation for a limited period of time,
it has successfully demonstrated the evaporation of rum slops (58)
Many initial scaling and fouling problems, however, were encoun-
tered.  During initial start-up, one of the stages was in a con-
tinuous down-phase for cleaning.
     In the United States, evaporation  has been applied to beet,;'
                                                                 Jr
citrus, and cane mojasses wastes from yeast, pharmaceutical, and"
                  <*
rum manufacturing.-faci 1 i ties (58).  Standard Brands Industries
(S8I) successfully operated an evaporation system at its yeast
producing facility in  Peekskill, New York.  The primary waste

                            VIII-5

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stream from the facility consisted of the spent molasses slops
remaining after the fermentation process in the production  of
yeasts.  The raw material for the yeast production consisted of
varying amounts of cane and beet molasses.  Restrictions in the
supply of beet molasses were such that SBI on occasion used only
cane molasses as their raw material.  Although SBI initially
experienced difficulties with the evaporator when only cane molas-
ses was used, they could evaporate cane molasses wastes by imple-
menting some specific improvements to their system designed to
reduce the scaling (58).
     The Red Star Yeast Plant in Belle Chase, Louisiana, produces
yeast from Brazilian cane molasses.  Red Star evaporates the waste
to CMS and the evaporator needs to be cleaned only once every two
to three weeks (53).
     Pfizer, a pharmaceutical manufacturer in Groton, Connecticut,
operates a large and technologically sophisticated evaporative
system for a beet molasses waste stream and only has to clean the
evaporators once every two weeks using caustic soda and nitric
acid (58),
Process Performa-nce
     The overall evaluation of evaporation technology is a func-
tion of several parameters, the more important of which are:
     •  Effect of process in reducing pollutants
     t  Operating-'eff iciency of equipment
     •  Energy consumption •
                             VIII-6

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Effect of Process in Reducing Pollutants--
     According to the testimony of Erik Krabbe, Region II EPA
staff consultant to the Facilities Technology Division (60). the
rum producers are required by EPA to remove approximately 94 per-
cent of their waste-load. BOOg.  The Malaga, Spain, Bacardi rum
plant is capable of 98.7 percent BODg reduction (58).  Some data
from Florida Distillers in Lake Alfred, Florida, consisting of
seven samples taken between May 17 and 19, 1976, showed a mean
30D5 reduction of 96;.2 percent (53).  Unfortunately, the Sols
Distillery does not measure SOOg removal.
     Puerto Rico Distillers (15) has noted that the evaporation
process performance at Sols and other distilleries is greatly
affected by whether or not the yeast and the fermenter bottoms
are removed from the mostos stream. .At Bols they must be removed
to prevent rapid clogging of the evaporator.  Puerto Rico Dis-
tillers has also noted that these high 800 sidestreams  cannot
be discharged untreated to the ocean without violating the 300^
limits of their NPOES perm'it.  Therefore, these yeasts and fermen-
ter bottoms must either be discharged to a POTW (an option not
available to any of the distillers - see Chapter V   , "Disposal
to a Public Owned Treatment .Works (POTW)"} or may require fur-  ••
ther treatment.                                                 :'.
                                                                *"S
     The production--of CMS results in large quantities of evapor-
                  /»
ator condensate re'portedly having a SOD value of 100 to 2,000
pom (32).  Supplemental biological treatment of this wastestream
may, therefore, be required prior to discharge.  Erik Krabbe,

                            VIII-7

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however, has stated that biological  treatment of the evaporator
condensate is not needed to meet the effluent standards required
by EPA (60).
Operational  Efficiency of Equipment--
     From a  practical  standpoint, the ability to successfully
evaporate mostos depends upon the nature of the input waste,
the desired  output, and the operating conditions to achieve this
output.
     It  is generally accepted that the evaporation of cane molas-
ses stillage results in more severe  scaling than either beet or
citrus molasses.  For  example, the Standard Brands operation in
Peekskill, New.York, was basically designed to evaporate beet
molasses.  Their six-stage multiple  plate evaporator, when pressed
into duty on only cane molasses (a condition which occurred for
a two-week period when beet molasses was not available), showed
evidence of  even more  severe scaling and fouling than normal (88).
Standard Brands, in more recent operations, has switched to
falling-film tube type designs (88).
     Similar experiences were reported by the Zuid Nederlandse
Spiritusfabriek  at Delfzil, Netherlands (ZNSF) (15).  This plant
normally uses beet molasses,but on several  occasions - two months
in 1974  and  one week i.n 1977 - the plant had to exclusively use .•
cane molasses.   On the first occasion, six  to eight' hours of
                   ,'                            j
cleaning were requ'ired after running the evaporator for 24 hours.
During 1977, they were able to operate 10 to 15 hours before
cleaning, a  sitation which more closely approximates the Bols
cycle (15).
                             VIII-3

-------
     To alleviate  the  fouling  problems,  Standard  Brands  was
forced to modify their  equipment.   Their  original  APV-designed
evaporator was  not able  to  concentrate  beyond  40-percent solids
without experiencing problems.  To  overcome  the basic  design  limi-
tations,  they  installed  a  two-stage  falling-film  tubular evapora-
tor  to achieve  final concentration  to  55  percent.
     Regardless of evaporator  type,  however, certain cleaning  pro-
cedures must be employed on  a  regular  basis  to  alleviate the  con-
tinual build-up or inorganic and  organic  deposits.  Typical ly, both
nitric acid and caustic  soda are  employed  for  removal  of these
deposits.  In  cases of  more  severe  scaling,  mechanical cleaning
has  to be employed.
     Another factor affecting  efficiency  is  the desired  solids
content of the  final product.   Whereas  CMS derived  from  beet  molas-
ses  may be taken to Q'5-70°  Srix,  CMS from  cane  stillage  has been
generally limited  to 55-60   Srix.   Above  this  level, fouling  and
.scaling become  more pronounced  and  viscosity increases to  a point
where  the final product  becomes difficult  to handle.
Energy Consumption--
     This was  discussed  in  the  section  entitled  "Theory, Design,
and  Operation."                                   ..          .
Pilot  Installation
                                                                 $
     Bacardi has bui-lt  a pilot-scale evaporation  system  at  its
                   <•
distillery in  Cataho,  Puerto Rico,  with  an operating capacity  of
approximately  30,000 gal/day (87).   Bacardi  has said that  if  the
                             VIII-9

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pilot tests are successful  and a method can be found for disposal
of CMS, they may build a.full-scale system (34).  The pilot tests
involve experiments with:
     •  Construction materials
     t  Pretraatment alternatives
     •  Cleaning cycles
     •  Temperature and other parameter optimization
     •  Condensata treatment
     •  CMS marketing (34).
     Bacardi's pilot-scale  evaporator, a two-body, three-stage
type, was designed by the Dedert Corporation. The first two stages
are falling film; the final  is forced recirculation . The system
was designed to operate on  the principle of mechanical  vapor
recompression (MVR).  Bacardi believes that this will be the
best-suited evaporator for  treatment of its waste from the stand-
points of cost and energy savings (34).
     In Puerto Rico electrical power is six times more expensive
than steam energy.  Therefore, Bacardi has opted to use a steam
turbine power generator to  drive the MVR system rather than elec-
tricity (34).
     It was calculated that  the Bacardi pilot MVR unit would
require about 2.2 KwH/1 ,000  Ib water removed, while the Bacardi,'
Malaga, multiple-effect evaporator currently requires about 112
KwH/1,000 Ib water removed  (57).
     Bacardi will be the first to evaluate its efficiency in a
rum distillery for the treatment of cane molasses.

                             VI11 -10

-------
     The construction of the pilot evaporation unit was completed
in August 1977, but the contractors were unable at that time to
furnish the turbine for the MVR.  The unit was consequently run
with steam until   July, 19-78,  at  w&Tcft time  the  MVR  mode  became
operative.
     During the break-in runs, there was some evidence that scale
build-up was reduced, permitting longer operation periods between
cleanings.  In one- of the early runs, for instance, the unit
developed some encrustation .after 120 hr of operation, and cir-
culation with a caustic solution did a fair job of cleaning (87).
It should be noted, however, that the long lengths of the initial
runs were chosen  to study the outer limits of operation and may
not represent the most practical production-to-cleaning cycle.
Furthermore, the ash content of the molasses used was only 10
percent, whereas  the average ash content of the molasses used by
Bacardi is approximately 12 percent (87).
     Certain mechanical problems were also experienced during
several of the test runs, but these were corrected between the
runs.  It was suggested that molasses pretreatment might provide
a waste that would be easier to evaporate, .but as  of October
1978  this step has yet to be implemented (87).
     The evaluations of both the evaporator and the MVR unit are-'
forthcoming from  the Bacardi Corporation, but are  not anticipa-
ted until sometime" after December 1, 1978.  Until  such a report
is 'issued, a detailed analysis of the units'  performance is not
pos si ble .

                             vrn-n

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CONDENSED MOLASSES SOLUBLES (CMS)
UTILIZATION AS AN ANIMAL FEED SUPPLEMENT
     The term "CMS" refers to a number of different products,
all  of which are made from the residue of a molasses fermenta-
tion process.  Molasses fermentation is involved in the produc-
tion of yeast, Pharmaceuticals, citric acid, monosodium glutamate,
alcohol (for both industrial  and beverage use), and various other
products.  During the manufacture  of these products, the fermen-
table sugars contained in the molasses are consumed by micro-
organisms., leaving a residue  which could be described as sugar-
less molasses.  This residue  is converted into CMS by evaporation
until a concentration of 55 to 60  percent solids is attained (35).
     The composition of CMS varies greatly depending upon the
type of molasses used and, to a lesser extent, the type of fer-
mentation process and end product  involved.  The single most
important determining factor  in the composition of CMS is whether
the  input material is cane or beet molasses.  In addition, there
is considerable variation when cane molasses is used because
cane molasses varies greatly  in composition, depending upon the
weather and soil conditions where  the cane was cultivated, the
method by which the cane is harvested, and the efficiency of the
sugar-refining process, of which molasses is a by-product (35). '$
     Beet-based CMS is much more desirable as a feed additive
than cane-based CMS because the protein level is much higher than
that of cane--approximately 18 percent for beet as opposed to
4 to 6 percent for cane (35).

                             V ILL-J2

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     Animal feed supplements are widely employed in the raising
of cattle, sheep, poultry, and other domestic animals (40). These
supplements extensively utilize by-product materials such as
molasses, fat, and urea (40,100).  CMS, derived from sugar cane,
sugar beet or citrus, may be substituted for part of the molasses
in some animal feed supplements (35T) .   In this manner, not only
the CMS is utilized, but limited supplies of more expensive
molasses are extended (35).
     CMS potentially could be employed in dry supplements and
liquid supplements.  .There ar.e technical  difficulties however,
in utilizing CMS in dry and block form supplements. Little CMS
(as a molasses substitute) can be used in feed blocks because-
technical problems related to the consistency of molasses have
severely limited its incorporation into solid blocks (40).  There-
fore, it appears that utilization in liqu.id and dry feed supple-
ments offers the most favorable potential for marketing large
quantities of CMS.
     Liquid supplements are available which contain a large per-
centage of molasses for high energy content.  The molasses con-
tent of a finished liquid supplement may be as high as 70 percent
by weight (solids basis).  The higher the molasses concentration,
the greater the stability of the liquid supplement (40).        :.*
     The trend in feed supplements has been away from dry sup-
plements to liquid forms because the latter are based on low cost
non-protein nitrogen sources such as urea instead of more costly
natural proteins such as soybean.  Fluid materials also have cer-
tain advantages in blending, handling, and transport operations (100).
                              vrrr-i3

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Literature  Review:  Feed Acceptance and Nutritional Value
Feed Acceptance--
     The results of some relatively recent animal feeding experi-
ments are summarized below.
     A progressively severe condition of black and watery excre-
ment was noted in hens which consumed rations containing more
than 10 percent mostos.  The appetite, weight, and appearance of
the birds remained healthy although, the watery excretions stained
eggs, hens, and surroundings (29).
     CMS levels of 0, 1, 2.5, 5, 7.5, and 10 percent were incor-
porated into broiler starter diets (27).  The eight-week body
                                      ^
weights of all birds receiving CMS were  numerically superior to
those of the controls, with those of the 7.5 percent treatment
being th-e highest.  Daily feed intake increased as the dietary
level of CMS was increased.  Feed conversion efficiency was sig-
nificantly lower than the control for the 7.5 percent level of
supplementation (27).
     Diets containing increasingly higher levels of CMS were also
fed to laying hens (27).  Statistical evaluation revealed a sig-
nificant egg production improvement associated with the feeding.
of 2.5 percent or more of CMS.  Egg weights and Haugh unit scores
(interior egg quality) were significantly reduced when 2.5 per-
cent or more of CMS-were fed.  Specific  gravity of eggs (shell
quality) and mortality were not significantly related to diet.
There was a trend toward increasing feed intake as CMS levels
were increased.  The investigator concluded, on the basis of
                             VIII-14

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these  data, that CMS appeared to be an acceptable and nutritious
product for use in diets of broilers and laying hens when proper
attention was given, during the diet formulation, to its overall
nutrient' content (27).
     Under wire cage management practices,  levels of cane molasses
as high as 20 percent can be incorporated in a practical diet for
laying hens (96).  But the average poultryman in Puerto Rico has
his flocks under floor management, where molasses levels over 15
percent can cause a problem with, caked litter.  Another factor
which would limit the use of locally-produced cane molasses in
commercial laying diets is its cost, when compared to that of
corn or other suitable more nutritive substitutes (96). Since
there is a similarity between cane molasses and CMS, this suggests
an upper lim-it of 15 percent CMS incorporation into poultry diets.
     Dried mostos (10 percent moisture) was tested as a feed
ingredient (corn substitute) for laying hens (97).  Above a level
of 20 percent, a serious detrimental effect on egg production
appeared.  Average egg weight, hen weight,  broken-open egg height,
and shell thickness were not affected by dry mostos levels as
high as 35 percent.  This tended to disprove the theory that
high levels of potassium, magnesium, and sulfates in the mostos
                                                                 r,
could have toxic effects such as the inhibition of calcium metabo^-
lism.  Most of the-^aggs produced by hens consuming levels of dry
mostos above 15 percent were stained, and the animals themselves
were soiled.  The overall data indicated that in practical diets

                              vrrr-i5

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for laying hens, the level  of dry mostos should not exceed 15
percent (97).
     The effects of feeding three percent dried molasses distil-
lers solubles  to steers in  all-grain rations was examined in
Australia (39).
     Spray-dried mostos has been used in cattle nutrition as a
supplement in  block or liquid form under grazing conditions.
Incorporation  in the feed caused a decrease in animal  live- and
carcas-weight  gain and an inferior feed conversion ratio (39).
     Forty Angus and Angus  X Hereford yearling steers  were
assigned to four treatment  groups in a completely randomized
design for an  84-day feeding trial (2).  Condensed molasses
solubles were  substituted in a corn-soybean meal diet  at 0, 5,
10, and 15 percent on a dry weight basi's.  The CMS were obtained
from a cane-sugar molasses  distillation operation.  After 56
days, steers fed 15 percent CMS were changed to the control diet
due to reduced  weight gain  and feed efficiency.  CMS at 5 and
10 percent also  decreased average daily gain and feed  conversion
efficiency, however, dry matter intake was  not influenced by
                                                 •
treatment.  At  84 days, there were no differences in average
daily gain or  dry matter intake for steers  fed 0, 5, and 10 per-
cent solubles,  but feed conversion efficiency was reduced.
     CMS had no  effjact on carcass quality or yield.  Chemical
analysis of CMS  simples revealed high levels of potassium (11.6
percent, dry matter basis).  CMS were judged to be le-ss valuable
than corn as an  ingredient  for finishing cattle.   They were

                             vrrr-16

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judged as useful when maintenance of animal weight or  less  than
maximum gains were desired (2).
Nutritional  Value--
     The mean chemical composition of several  cane CMS samples
is presented in Table VIII-1. The inorganic ash fraction comprises
approximately 30 percent of the dry mattar of the CMS.  Protein
comprises approximately 9 percent.  The potassium level is  very
high.  An amino acid analysis of cane CMS is also presented in
Table' VIII.-2 .  This  data  is  based on  the mean of only two samples
but it compares, well with data presented elsewhere (68) for
"molasses ethyl alcohol  fermentation solubles."  Several of the
amino acids  are present in relatively high concentrations.
     The quality of molasses stillage as a feed supplement  is
improved by  desalting and replacing potassium with ammonium ion.
(94, 102).  There are several  variations in the desalting tech-
nique but generally sulfuric acid and gypsum are added to con-
centrated stillage at room temperature.  The potassium content
is reduced by 70 percent and the-ash content in dry matter  by
40 to 45 percent.  The content of nitrogen-containing  substances
is increased by 30 percent (65 , 172).
Potential for CMS Use in Puerto Rico and the U.S. Virgin Islands
     The preceeding section has presented data indicating that "^
CMS can be successfully utilized when incorporated into animal
                r*
feed rations at certain low percentages.  This may be  performed
by local feed manufacturers (on the islands) or, alternately,
CMS can be exported in bulk to one of several  destinations  -

                             VIII-17

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    -TABLE VI.I'I-I.   M£AN CHEMICAL COMPOSITION OF CANE CMS'

   Component                            Concentration
Dry Matter (?)                              52.56
Ash (%)                                     32.63
Crude Protein (*)                            8.69
Ca (%)                                       2.06
P («)                                         0.16
K (%)                                        11 .59
Ma (%}                                       0.84
Mg (%)                                       1.07
Fe (ppm)                                        1865
Mn (ppm)                                    71 .7
Cu (ppm)                                    115.5
Zn (ppm                                     56.9
  Dry weight basis  (2) .
                             VI IT-18

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      TABLE VILI.-2.  AMINO ACID ANALYSIS OF  CANE  CMS*
        Ami no Acid                   Concentration (%)
        Aspartic Acid                      .791
        Threonine                          .125
        Serine                             .144
        GTutamic Acid                      .403
        Glycirve                            .118
        Alanine                            .200
        Valirie                             .159
        Cystine (Cysteine)                 .026
        Methionine                         .024
        Isoleucine                         .101
        Leucine                            .146
        Tyrosine                           .072
        Phenylalanine                      .086
        Lysine                       •      .089
        Histidine                          .046
        Arginine      .                     .069
Dry weight basi s (2 ) .
                           vrrr-i9

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United States, South America, etc.  This discussion will only
consider the potential  for local  consumption.
Approach--
     The potential  for  local  CMS  consumption is a function of
both the animal  population and the amount of locally formulated
feed supplements.   According  to Soldevila the only likely vehi-
cle for incorporating CMS into the diets of local animals is in
feed concentrate prepared and sold by local commercial  feed
mills (73).  Therefore, estimates of the potential CMS  market
                                                   t
must be dependent upon  the quantities of feeds manufactured.
Since agriculture is virtually non-existent on any of the three
U.S. Virgin Islands, this analysis will  be confined only to
Puerto Rico.
     A degree of uncertainty  exists relative to the maximum
amount of CMS that can  be tolerated by cattle, swine,and poultry.
Many of the feeding studies cited in The Literature Review sec-
tion of this chapter have been conducted with, in some  cases,
mostos (either dilute or spray dried) or with CMS.  These tests
are not readily translatable  into acceptable maximum or minimum
ration levels.  For the purpose of.estimating CMS consumption,
therefore, several  levels of  incorporation were used -  5, 10, and
15 percent.  Fifteen percent  was  chosen  as the maximum  allowabVe
level for feed incorporation  as suggested by Dr.  Soldevila's tes-
timony (73), in which he discussed homogeneity mixing problems
with liquids used as supplements  to dry  feed.  While Or. Solde-
vila's remarks were directed  toward molasses incorporation, it

                            vrrr-2o

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is assumed that CMS would offer similar mixing problems.   Or.
Soldevila reports that the feed mills in Puerto Rico are only
incorporating five percent molasses  into feed concentrate  (73).
Therefore, to assume a percentage of more than five percent CMS
presupposes that the feed mills could change their mixing opera-
tions.
      In 1975, a reported 423,057 T of animal feed were produced
loca-lly in Puerto Rico (73).  Using  this figure as a starting
point, Table  VIII-3 presents  the estimated  maximum annual  amount
of CMS which  could be used at three  differing levels of feed
incorporation.  Estimates were based, in part, in the following
assumptions:
      t  All feed mills in Puerto R.ico would  incorporate CMS
        into  their respective production
      ••  The. constituents contained in CMS are not harmful  to
        the consuming animals up to  the 15  percent maximum
      •  CMS is 60 percent solids, a  percentage at which bacterial
        activity is known to  be negligible  (32), and was con-
        centrated from mostos at 8.35 percent total solids.
      As can be seen from Table VIII-3,  roughly 50. percent  of  the
total CMS produced could be consumed locally if utilized at
the  15 percent level.  This is a hypothetical maximum.  In      •%
reality,  the  total ^amount of  CMS consumable  through feed incor-
poration  would probably  be closer to the 21,000 T shown at the
5  percent level.  This allows for both marketing and diet  prob-
lems, surely  to be encountered when  initially utilizing a  waste
by-product as a food supplement.

                             V111 - 21

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               TABLE VI11-3.   ESTIMATED MAXIMUM ANNUAL QUANTITIES  OF
                       CMS WHICH COULD BE INCORPORATED INTO
                          PUERTO RICAN MIXED ANIMAL FEED*
         Parameter
Ouantity'of feed mixed in
  Puerto Ri'co
Quantity of CMS
  supplement
used at 5%
Quantity of CMS used at 10%
  supplement

Quantity of CMS used at 15%
  supplement

Total  annual quantity of CMS
  (60% solids)  produced by
  Bacardi,  Puerto  Rico  Dis-
  tillers,  and  VIRIL
                   Incremental  Subtotal
                                                               -Tons
Total
                                                          423,057
                         21 ,153


                         42,306


                         63,459
                                                          114,813
  Based  on 1975 feed figures  (73)

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USE OF CMS AS AN INTERMEDIATE' PRODUCT
Introduction
     The previous section discussed the use of CMS as an end
products in itself - i.e., as a potential  animal  feed supplement.
CMS, or a similar product with lower solids content, can also
be utilized as an intermediate product, from which certain by
products can be racoversd.  Utilization of CMS as a intermediate
product in the following processes will be discussed.
     ••  Incineration - Control Parameters
     •  Incineration - Equipment Options
     •  Cost's of Evaporation/Incineration
     •  'Potassium Recovery
     t  Organic Fertilizer Production
Control Parameters for Incineration
     Incineration of mostos may be accomplished either directly
in the waste stream or after some degree of concentration.  The
advantage of concentration is anticipated  cost savings, on both
total equipment investment and operating costs.  Engineers and
manufacturers of incinerating equipment have been quoted as say-
ing that it would be several times more expensive to incinerate
slops directly than to pre-concentrate and then incinerate (15).
Therefore, any cost-effective incineration plan is likely  to   "*
include an evaporation step.
                 «
      Important  control  parameters  which should be  considered  are
 the  incinerator  feed  solids  concentration,  the temperature in

                              vrrr-23

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the reactor, and the excess air supply.  Calculations performed
on an analysis for a typical  mostos stream indicate that auto-
genous combustion (combustion without the need for a continuous
supply of auxiliary fuel)  can take place with a feed total  solids
(TS) concentration as low as 35 percent (61).  An analysis  was
not performed to determine whether auxiliary fuel was needed to
initially bring  the incinerator up to its normal  operating  tem-
perature, or whether the  CMS could serve as start-up fuel.
     A TS concentration significantly above 35 percent is desir-
able to increase heat recovery in a waste heat boiler (61)  and
to decrease the  size of the incinerator.  A CMS concentration
of about 50 percent TS seems optimum since at higher concentra-
tions technical  problems  with evaporation conditions and •
pumping characteristics of the CMS arise.(61).  A further dis-
advantage of having a higher concentration is due to the fact
that the incinerator temperature must be maintained below 700 C
in order to prevent ash fusion.  Pilot plant work (10) showed
that with a 75 percent TS  feed, maintaining the incinerator
temperature below 700 C resulted in the need for  higher excess
air conditions.   In order  to supply a large amount of excess
air, a larger sized reactor is required than would otherwise be
necessary.
     The need for maintaining the incinerator temperature below'
                  ,»
700 C is due to  -the low melting point of the inorganic salts
contained in the waste.  In general, the need to  strictly con-
trol the temperature causes difficulty in attaining the correct

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balance of operating conditions for a suitable combustion tem-
perature with a complete combustion of volatiles (51).  The dif-
ficulty of stabilizing the operating conditions is accentuated
by variations in the organic and inorganic composition of the
waste.
     The variability in the waste composition is related to the
variations in the molasses raw material.  Molasses is a by-product
of the sugar industry and is affected by variations in the ori-
ginal  sugar cane source, in the sugar processing,  and in the
sugar  mill efficiency.  Its characteristics,  accordingly, vary
widely and cannot be completely predetermined or controlled by
the rum producers.   Bacardi Corporation,, especially, because of
fts""Targe volume of.product, must rely on many molasses sources.
     Several variations in types of incineration systems are
available, as each  manufacturer tends to have their own innova-
tions.  Few of these have been applied specifically to rum wastes.
However, wastes produced in the paper manufacturing industry
which  are somewhat  similar to rum slops, but  containing sodium
rather than potassium salts, have been concentrated and fully
oxidized in a conventionally operated fluidized bed incinera- ..
tor (61).  In 1915  rum slops were concentrated and incinerated
in the Porion Furnace and a system was later  developed by Whita-,
                                                                >
ker (117) and U.S. .Industrial Chemicals, Inc.  (4a ,   43).  During
                 <•
the early 1940' s-'Reich (83) carried out experiments in low tem-
perature carbonization retorts at 343°C for the production of
carbon and potash.   Further work- was carried  out in the distillery

                            VI11-25

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of the Dyer Meaking Breweries Ltd., in Lucknow, India, in 1960
with a pilot-scale multiple hearth-type incinerator (1Q).
     Kujala, et al. (61) have described an alternative to a
conventional fluidized bed incinerator, consisting of using the
fluidized bed reactor as a gasifier and performing the after
burning in an after combustion chamber which is linked to a
waste heat boiler.  Apparently, the advantage of this type of
system over a conventional single reactor system is that it alle-
viates the difficulty of maintaining the proper balance of opera-
ting conditions for a suitable combustion temperature while
obtaining a complete combustion of volatiles.  In the alterna-
tive fluidized system, the temperature in the gasifier is regu-
lated below the melting point of the ash, but is sufficient for
the volatilization of organic materials.  Gases and volatilized
organics are separated from ash and are fully oxidized in the
after combustion chamber at a temperature which need not be
below the ash fusion point.  Several incineration systems are
manufactured which utilize this same principle, having a primary
and secondary combustion chamber.  Most of these however, are
not of the fluidized bed type, and therefore, do not require a
cyclone separator for ash separation.   By providing near-complete
combustion of gases and organics in the secondary chamber, they'
have the ability to minimize air pollution.   In fact, some of
them reduce polTutant emissions to the extent that the systems
can be installed without scrubbers.  Combustion systems based
on this design are called controlled air incinerators.

                             VII1-26

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Costs of Evaporation and Incineration
     The costs of evaporation alone have been discussed previously
under "Evaporation Technology."  The same cost considerations
apply here.  In addition, in a combined evaporation and inciner-
ation system there is an interrelationship between heat recovery
during incineration and fuel costs for evaporation.  An attempt
to determine the costs of an evaporation and incineration system
depends to a. great extent on the accuracy of information with
regard to fuel consumption for both processes and on the poten-
tial  for the recovery and use of heat energy in place of fuel.
Accurate information on the net energy requirements for evapora-
tion and incineration of rum slops is lacking.
     George H. Oorion of Bacardi Corporation has evaluated pro-
ject designs for two types of incineration systems (34). The
first, the Copeland system, is a modified type of fluidized bed
system.  It involves pre-concentration of mostos to 34  8rix.
During pilot tests with this method, auxiliary fuel was neces-
sary to sustain incineration.  It was possible to incinerate
48 gal of slops/gal of fuel during the best test run.  The.
investment cost for this system was estimated at $1,075,000 for
a unit processing 73,000 gal mostos/day.  The operating cost/1,300
gal  slops was estimated at $20.00.
     A second system, the Prenco incineration system, was evalu—
                  /*
ated and pilot tested.  Being a liquid incineration system, it
required auxiliary fuel because of the low BTU/gal available
from mostos.  The investment cost was estimated at $476,000- for

                             vr.rr-27

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a 33,600 gal mostos/day.  The estimated operating cost/1,000
gal  mostos was $24.40
     Neither the Prenco nor the Copeland system designs were
based on maximum waste heat recovery, and neither included costs
for air pollution control or ash handling.  Efficient waste heat
recovery would be expected to lower the net costs of treatment,
while air pollution control and ash handling requirements would
raise them.
     Puerto Rico Distillers has estimated costs for evaporation
followed by a Brule  Incinerator (15) which include costs for
ash  handling and air pollution control, but again do not include
the  savings possible with efficient, heat recovery.  These costs
are  much higher on a per gallons basis than Bacardi's estimates
for  the Prenco and Copeland systems.  This is suprising because
the  former system involved production of CMS (50° Brix), while
the  two latter systems did not.  Puerto Rico Distillers has
stated that manufacturers who quoted them incineration equipment
costs agreed that it would be several times less  expensive to
incinerate CMS than the dilute slops (15).  Yet the estimated
capital expense of an evaporation and incineration system was
$4,655,000 for a 223,200 gal  mostos/day, and the operating
cost was estimated at $47.00/1,000 gal slops.  The corresponding
                                                                •>
breakdown of costs ^.all ocated  $400,760/yr for fuel oil for inci-
neration (of ato'tal direct cost for incineration of $579,440).
This fuel oil cost is based on 1.268 mil gal/yr at $0.32/gal.
This allocation is extremely  high, amounting to 5,283 gal  fuel
oil/day.  In addition, $284,400 was allocated for fuel  oil  for
                             virr-28

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evaporation, or  3,750 gal/day.   In  total,  9,033  gal  fuel/day
wars allocated for evaporation and incineration,  equivalent to
25 gal slops treated/gal fuel. In contrast,  the Copeland  system
described earlier indicated that  it  was  possible  to  treat  a.s
much as 48 gal mostos/gal fuel.   Puerto  Rico  Distiller's  estimate
should be at least as low as  48 gal  slops/gal fuel;  and  in  fact
with the type of system  proposed  by  Bacardi,  fuel  for  incinera-
tion should be minimal except during  start-up.  While  it  IS not
possible to cal cul ate'the amount  of  fuel  required,  it  is  estima-
ted, that approximately 1,000  gal  fuel would  be  a  maximum  require-
ment for incinerator start-up.  Assuming  that CMS is  autogenous,
auxiliary fuel consumption  to .sustain.combustion  should  be  mini-
mal as compared to Puerto Rico Ois-tillers1  estimate,  of  5,283
gal/day.
Potassium Recovery from  Incinerator  Ash
     Evaporation and subsequent incineration  is expensive  in
terms of plant investment and operating  costs.  Therefore,  it
is essential to obtain a return on the capital  expenditure  if
possible.  The incinerator  ash is itself  a  by-product which has
a potential value as a fertilizer component;  however, by means
of further processing a  more  purified product may command  a
higher market price.                                           ::
                                                               *..
     The incinerator ash is basically a  crude potash containing
                 /*
potassium salts a'nd impurities such  as calcium  and magnesium
salts, silica, traces of iron and manganese,  and  unburned  carbon-
aceous particles (61). Ash  has been  reported  to contain  37  percent

                             vrrr-29

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potassium (expressed as K20)  (59, 16).  According to Kujala,
et al .  (ST)  potassium salts generally should comprise 65 to 70
percent of the ash.  Expressed as a percentage of the ash, the
composition  of salts is typically KpSCL, 56 percent; KC1 ,  7.0
percent;  and K-CO,, 5.0 percent.  Some investigators (37)  have
              u  0
reported  K2C03 contents of as high- as- 50 to 60 percent, with
only 15 percent K^SO,,  and 10 percent KC1 .
     The  use of incinerator ash as fertilizer component, in con-
junction  with nitrogen- and phosphorus-rich chemicals, is  prac-
ticed  at  Izumi, Japan (24).  A plant belonging to the Japanese
Ministry  of  International  Trade and Industry producing non-
beverage  alcohol  incinerates  some of its CMS.  The ash is  a fine
slate  gray powder which is crushed in a mill and then sold to
a dealer.  The dealer resells it as such or, more often, mixes
it with other materials to make a fertilizer.  The plant repor-
tedly  sells  the ash for about $21/t  and the dealer resells it
for about twice that price, or .still  higher if it is converted
to a fertilizer.
     Work on the  recovery  of  potassium salts has taken place in
India  (10, 61).  A 90 gal/hr  pilot plant consisting of evapora-
tion,  incineration, and potassium salt recovery was constructed
at one of the Indian rum distilleries.  Fresh slops were neu-
tralized  with lime to pH 7.0, filtered in  a filter press,  and
fed to. an evapora-tor through  a heat exchanger.  CMS at 75  per-
cent dry  solids was produced  and fed to an  incinerator where it
was ashed.  The ash was removed and leached with water for the

                             vnr-3o

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dissolution of all  the potassium salts.  The solution was fil-
tered and neutralized with sulfuric acid to convert the carbo-
nates to sulfates.   The neutralized liquor was concentrated  in
an evaporator to 25 to 30 percent dry solids.  Potassium salts
were crystallized out from the concentrate in a screw trough
crystal 1izer.  A basket, centrifuge followed for separating sus-
pended crystals from magma.  The crystals were then dried and
bagged,  while the magma was reused in the process.  The finished
product contained 83 percent potassium sulfate; 9 percent potas-
sium chloride with  traces- of sodium salts; and 8 percent mois-
ture.  A flow diagram of the entire potassium salt recovery  pro-
cess is  shown in Figure V'IIL-1 .  .
     Based on this  research, it was estimated that a distillery
producing 72,000 gaT rum slops/day, could recovery 3.85 ton/day
of potassium salts  (expressed as K20).  At 1964 prices for
potassic fertilizers, the cost of the treatment plant could  be
recovered in an estimated 5 to 6 yr from the sale of the by-
product (TO) .
     Kujala, et al. (ST) state that the factory selling price
for a refined potassium salt by-product could be on the order
of $200/t within a  few years.  They report that there are indi-
cations that world  resources of potassium salts, essential in  ;*
N/P/K fertilizer formulations, are tending to reach a finite    ~~
                 S"
limit in avai 1 abfl  i ty, with anticipated future shortfalls in
supply.   Kujala, et al. (61) state that potassium for fertili-
zer needs is normally sold as KC1 with a smaller market for

                            vrri-3i

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        CRUDE POTASH
           3-STAGE
         LEACHING TANK
       (COUNTERCURRENT
          EXTRACTION)
            DUPLEX
            FILTERS
                   FILTERED
                   SOLUTION
          NEUTRALIZING
             TANK
   NEUTRALIZED
     FILTERED
     SOLUTION
   MAGMA
           SHELL AND
           TUSE HEAT
           EXCHANGER
   HOT
EVAPORATED
  LIQUOR
   SCREW
   TROUGH
CRYSTALL1ZER
          EVAPORATOR
   MAGMA AND
   SUSPENDED
    CRYSTALS -
              SOLIDS FOR
               DISPOSAL
  BASKET
CENTRIFUGE
       SALT
    CRYSTALS
                      DRUM
                      DRYER
          CONDENSATE
                    STORAGE
                      AND
                    BAGGING
                                              FINAL PRODUCT
    .Figure VIII-1.   Potassium. ..S,a\H Recovery From Crude Potash.
                           VIII-32

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K-SO, (the major potassium salt recoverable from rum slops).
With certain crops and in many tropical areas where soil sali-
nity levels are high, the chloride ion is undesirable and sul-
fate of potash is preferred.   Quite often, these areas are to
be found where sugar cane is  grown.
     The market for potassium salts available to the Puerto
Rican and Virgin Islands rum  producers is uncertain. Table  VIII-4
presents estimated potassium  production figures based on the
following assumptions:
     •  Incinerator ash is produced at the rate of 0.2 Ib ash/
      .  gal of 8.35° Brix mostos
     •  Ash is 55 percent total potassium salts (37 percent
        expressed as. K-Q) .
Qf the total estimated .annual production of 15,000 T, 5,550 T
(expressed as 1^0) would be available for potential use as a
fertilizer source.  To place  this figure in proper prospective,
the potential local market for potassium needs must be examined.
     Table VIII-5 presents the annual  average consumption of
potassium chloride and other  potassium salts  by direct applica-
tion (not mixed with other nutrients)  in Puerto Rico over the
period 1965 to 1975 (109) .
                             vrrr-33

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TABLE VIII-4.  ESTIMATED ANNUAL POTASSIUM PRODUCTION
         AS AN INCINERATION BY-PRODUCT
•i
Dally Generation of
Mostos (gals/ day)
Operating days/yr
Ash Production (T/yr)
Potassium Production (T/yr)
(Expressed as K^O)
Remaining Residue to
be Disposed (T/yr)
Bacardi
300,000
300
9,000
3,330
5,670
Puerto Rico
Distill ers
200,000
200
4,000
1 ,480 .
2,520
VIRIL
100,000
200
2,000
740
1 ,260
Totals
600,000
--
15,000
5,550
9,450

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      TABLE VIII-5.  PUERTO RICAiN ANNUAL COMMERCIAL
                  CONSUMPTION OF POTASH AND
                 POTASSIUM COMPOUNDS IN DIRECT
                     APPLICATION MATERIALS*
Potassium Product              Annual Average Consumption  (Tons)'
Potassium Chloride, 50-62%
  Grade (expressed as KC-1 )                  1,110
Other Forms                                   586
                               Total        1,696
*' (109)
1  Based on 1965 to 1975 consumption figures.
     Assuming that the bulk of KC1 is in the normal 60 percent
grade, the 1,110 T would be equivalent to 802 T (expressed as
K^O).  Similarly, if the "Other Forms" are predominately K^SO^,
the 586 T could be translatable into 318 T expressed as K-0 .
The estimated <20 total of 1,120 T (802 +313) can  then be com-
pared to the hypothetical  production total  of 5,550 T (Table
VIII-1).  As ,can be seen,  production would exceed current use
by roughly five times.  Assuming that local  consumption would not
increase, the difference would have to be exported at a presumed
price disadvantage.                                            -^
Organic Ferti 1 i zer "Production from.CMS
     Molasses alcohol  stillage contains considerable amounts of
organic materials including amino acids, proteins, saccharides,
gum materials,  etc.  Kyowa Hakko Kogya Co., Ltd., of Tokyo and
                             vrri-35

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Hofu, Japan, has developed a process for creating an organic
complex N-P-K fertilizer from stilla.ge by converting the organic
components to humic acid (24).  The humic acid forms an  organic
complexing agent to which inorganic nutrients are coupled. This
coupling controls the release of the nutrients for plant growth.
Consequently, the damages caused by high concentrations of nutri-
ents often seen in inorganic chemical  fertilizers are minimized.
     Kyowa Hakko Kogyo Co. obtains sugarcane molas.ses in Japan
and from Indochina, the Philippines, and Taiwan.   The cane
molasses is converted into alcohol and is used in the production
of Pharmaceuticals and other products  at Hofu, Japan (24). The
company has performed experiments (44) which revealed signifi-
cant damage to germinating plants when untreated  cane molasses
stillage was applied to the soil.  However, if the stillage was
treated with mineral  acids, the damage was essentially elimina-
ted.  The damage was apparently caused by organic acids contained
in the untreated stillage or by the acids formed  in the soil
from the sugar component of the stillage. By treating with min-
eral acids, the low molecular weight organic compounds were
dehydrated, condensated, and converted to humic acid.
     A procedure for producing fertilizer from stillage involv-
ing evaporation to CMS prior to subsequent treatment steps was >
developed.  A pilot program using a plate-type triple effect
evaporator with 
-------
With five of these evaporators, the Hofu plant has produced
approximately 140,000 t/yr fertilizer (24).  The evaporators
produce CMS with approximately 50 percent total solids. In the
fertilizer plant, sulfuric acid is added to adjust pH. The mix-
ture Ms then heated to about 110°C for 8 to 10 hr as  it is
passed through a series of six - 300 gal reactors.  Ammonia is
added to achieve a pH of 4 to 5.  Finally, phosphoric acid and  '
potassium sulfate- are added to adjust the composition of the
final fertilizer.  The resulting mixture has a soupy  consistency.
An agglomeration process then follows, resulting in a granulated
but moist product.  The granules are dried in a direct-fired
(with heavy oil)   rotary kiln which is about 6. ft in diameter
and 70 ft long.  Treatment of the exhaust gasses from the rotary
kiln is required.  The dried granules are cooled and  put through
a vibrating sifter to obtain the final product.  A flow diagram
for this process, is shown in Figure VIII-2.
     The final product' has the desirable characteristics of
being homogeneous, granulated., and available in several grades.
It has been commercially sold for $212 to $220/t in Japan.  A
lUS. selling price of $160/t was proposed (24).  Kyowa Hakko  .
Kogyo Co. advised that this type of fertilizer production was
not a break even proposition unless annual quantities were in  v_
excess of 10,000 t/yr (24). They estimated that if VIRIL were to
employ this process, their annual production of fertilizer would
be approximately 7,000 t.  Assuming that this estimate was based
on a yearly production figure of approximately 3.4  million proof

                             Viri-37

-------
 I
' CJ
 CD
                                                       FERMENTATION
                                                        WASTEWATER
          [CA-  SUPER PHOSPHATE j [H3PQ4 | |KgSQ4j j NH3;
                                                                                 *REF. 58
        A

        B

        C

        D

        E
EVAPORATOR           F

GLASS LINED  REACTOR  G

NEUTRALIZER          H

KNEADER             I

MIXER               J
GRANULATOR           K

ROTARY DRYER         L

FIRST ROTARY COOLER  M

SIFTER               N

CRUSHER              O
                  PRODUCT
SECOND ROTARY COOLER

HOPPER

WASHING SPRAY TOWER

WET-TYPE COTTRELL DUST COLLECTOR

AFTER BURNER (INCINERATION)
                     .figure  VIII-2.   Process Flow Diagram  for  the  Production
                                      of An Organic Compound  Fertilizer*

-------
gallons, organic fertilizer estimates for Bacardi (.17.4 million
proof gallons) and Puerto Rico Distillers (5.2 million proof
gallons( would be 35,800 and 10,70.0 t, respectively.
                            VIII-39

-------
               IX.   POLLUTANT REDUCTION THROUGH
                       PROCESS MODIFICATIONS
     There are several process modification options available to
the rum distillers  which, if implemented, would effect partial
reductions of certain pollutants.  Those of significant
importance are:
     t  Pretreatment of molasses prior to fermentation
     »  Removal  and recovery of yeast from fermenter beer
     •  Removal  of fermenter bottoms
     •  Recovery of fusel oils and "heads" as a fuel
        supplement.
These are all options which the permittees have indicated that
they could implement quickly, i.e., within 6 mo to 1 yr (80).
Unfortunately, together, these methods are expected to give a
maximum BOD5 reduction of only 13 percent and TSS removals of
slightly more than  65 percent (80).  The SOOg reduction is so
low because only the insoluble solids are affected by these
methods, and approximately 80 percent of the waste 800^ is in
soluble form (93).  Nevertheless,, these options may be considered
as feasible because of the relative ease of implementation, the
possibilities for recovery of valuable by-products, and the posi-
tive effects on  subsequent mostos treatment options.
                                                                '£*•
PRETREATMENT  OF  MOLASSES
     Molasses pretreatment clarifies and pasteurizes the raw
molasses, removing gums, sludges, salts, and unwanted micro-
organisms.  These components tend to inhibit the yeast activity

-------
and, if not removed, will  become unfermentable solids in the
bottoms of the fermentation tanks (61).
     Molasses pretreatment may be achieved by diluting molasses
to approximately 40  Brix  and raising the temperature close
to the boiling point, when, due to the inverted solubility of
the calcium salts, mainly  calcium sulfate, maximum sedimentation
occurs.  Under these heated conditions,  separation can be
carried out in large settling tanks or by use of an ejecting-
type molasses clarifier.   After clarification, heat recovery
is possible by exchange with the incoming, freshly-diluted 40
Brix molasses (61 ).
     Molasses pretreatment has important side benefits.   Clarifi-
cation and pasteurization  of the molasses substrate yields a
cleaner alcohol; consequently, the purifying column in the
distillation unit requires fewer stoppages for descaling, and
reduction in the build-up  of sludges in  the boiling column per-_
mits 'longer operating runs.  Most importantly, from the  stand-
point of effluent treatment, the slops coming from the stills
are cleaner and would be  expected to cause less scaling  of
mechanical treatment equipment,, e.g., evaporation units  (61).
     However, there is a  large energy committment and capital
expense involved in molasses pretreatment (80 , 67 ).  In  addition-',
there is reportedly, some  1 oss of fermentable sugars ("80).  This
is more of a problem as molasses becomes more expensive, there
being a trade-off between  benefits gained from pretreatment
versus sugar losses (decreased rum yields).

                               IX-2

-------
     A partial  .pretreatment of molasses can be effected simply
by dilution and then centrifugation.   Some distillers prefer
this method, which does not involve heating,  or forced
sedimentation (67, 80).  Without heating, however, neither
pasteurization  of the molasses nor precipitation of calcium
salts is achieved.  Figure I'X-1  presents a typical molasses
centrifugation  operation.
YEAST RECOVERY
     Yeast may  be eliminated from the fermenter beer by
centrifugation  (Figure I'X-2).  The centrifuged yeast may then
be recycled for further use in the fermenters, sold for an
animal feedstuff (with or without drying), or land disposed.
     The Bols Distillery in Scheidam, The Netherlands, practices
yeast centrifugation and recycling "(24).  Yeast used in the
fermentation is removed from the. beer by a Westphalia centrifuge
and is recycled.   The separated yeast is acidulated with
hydrochloric acid, to a pH of 1.8 to 2.0 to prevent spoilage
during storage.  When needed again for fermentation, sodium
hydroxide is added to produce a yeast cream of pH "5, which
reportedly has  a total solids content of 10 percent and contains
45 percent protein and 3 percent ^2^5*  Urea  and phosphorus are
added as nutrients; fermentation is accomplished in about 12 hr/
                                                                '
The yeast is continuously recycled for 3 to 4 mo, at which time
it is discarded to the local sewer system (24).
                              IX-3

-------
               CONTINUOUS  IN-LINE
                     MIXING
MOLASSES
   WATER
          DILUTED MOLASSES
            STORAGE TANK
                         CENTRIFUGAL
                          SEPARATOR
   L
                                     LAND DISPOSAL
SLURRY PUMP
    SOLIDS RECOVERY
    TANK
                                      CENTRIFUGED
                                      MOLASSES STORAGE
                                      TANK
                                                               PUMP
                                               TO
                                       FERMENTATION TANKS
                  Figure IX-1 ,   Molasses centrifugatlon (80)

-------
                                     WATER
x
en
                                             PUMP
                                                 7
               YEAST CREAM    •'-YEAST  CREAM
             RECEIVING TANKS    WASHING TANKS
                                                     YEAST
                                                  CENTRIFUGAL
                                                   SEPARATOR
                                PUMP
  - YEAST
CENTRIFUGAL
 SEPARATOR
                 FERMENTATION
                     TANKS
                                                                                   .HYDROCHLORIC
                                                                                    ACID
                                             YEAST
                                             CREAM
                                       *   HOLD.ING
                                       '      TANK
                                                                                    PUMP
                           DISPOSAL
                          •~OF  DILUTE
                           SIDESTREAM
YEAST CREAM
TO PROCESS
                                                                             BEER TO DISTILLERY
                   CLARIFIED BEER
                     STORAGE TANK
              F1 gure/. I-X-2.   Yeast  recycling by centri fugation  of  fermenter  beer (80).

-------
     The advantages of yeast recycling are reported to

include:

     •  Shorter fermentation times (61)

     •  Increased alcohol  yield (61)

     •  Greatly diminished demand for new bakers'  yeast

     •  Short technology implementation  time (80)

     •  Less clogging of evaporation  equipment used for
        treatment of mostos (24)

     •  Reduction of the amount of yeast requiring disposal
        to a small  amount  that is wasted (80)

     •  Suitability of the wasted yeast  for land application
        or use as an animal feedstuff

     •  Availability of an alternative option  for  by-
        product recovery:   sale of yeast as a  high-
        protein animal feed supplement.

     Possible disadvantages of yeast  recycling include:

     •  Requirement for molasses  pretreatment  (6T , 67)

     •  Unknown and perhaps undesirable  effects  of molasses
      .  pretreatment on alcohol yield and/or quality
        (67, 80)

     •  Production  of higher temperatures in the fermenter,
        possibly necessitating installation of a fermenter
        chiller (80.)

     •  Requirement of land disposal  of  molasses pretreat-
        ment residue and wasted yeast.

     A particular version  of yeast recycling is  known as the

Melle-Boinet technique.  With this technique,  fermentation

times can be shortened to  14 hr,  with up to a  5  percent increase

in alcohol yield, according to Kujala, e_t_ ajk  (61);  without

yeast recycling, fermentation times can  take 24  to 72 hr (36).
                               IX-6

-------
With this technique, a high yeast concentration is maintained
in the fermenter and cell reproduction is almost eliminated,
transforming sugars previously used for yea'st growth into
alcohol.   Providing the yeast is handled properly and biological
infections in the fermentation are controlled, it can be
recycled up to 30 times before an appreciable loss in activity
occurs (  61).
     Use  of the Melle-Boinet technique in industrial spirits
manufacture can only be made where molasses has first been
pasteurized and pretrsated to remove components which inhibit
the yeast (61).  Therefore, there is the risk that sugar losses
will offset any gain in alcohol  production from increased
efficiency of sugar utilization.
REMOVAL OF FERMENTER BOTTOMS
     Distilleries have the option of eliminating suspended
inorganic and organic materials  (other than the live yeast) which
could become part of the rum slops in either of two ways:
by molasses pretreatment, which  has already been discussed, or
by the removal of fermenter bottoms.
     Fermenter bottoms are the unfermentable solids and dead
yeast cells which settle to the  bottom of a fermentation tank.
The sludge is both inorganic and organic, being derived mainly
from the impurities" of the original molasses.  Fermenter bottom
                 ?•
sludge is minimized if molasses  pretreatment is practiced;
otherwise, several hundred gallons per fermentation tank of a
high BOO sludge is formed (61).   When fermentation ceases, the
                              IX-7

-------
liquid containing the live yeast cells is drained off for
yeast recovery and/or distillation and the bottom sludge must
be washed out of the tanks before they are reused for fermenta-
tion.  A schematic flow diagram which shows how fermenter
bottoms are removed is included as Figure IX-3.
     It has been the practice  at many distilleries  to either
run this material down a sewer or to dispose of it with the
slops.   An alternative is removal via a sludge pump to a holding
tank and then land disposal  (80).  It is generally accepted that
this material cannot be treated with the rum slops due to its
effect on mechanical equipment, especially evaporation
equipment (15, 24).
     The advantages of removal of fermenter bottoms over
molasses pretreatment are reported to be lower capital and
operating (energy) costs and higher alcohol yields (80, 67).
If yeast recycling is practiced, however, alcohol  yields may,
in fact, be higher wi'th molasses pretreatment (61).
RECOVERY OF FUSEL OILS AND "HEADS"
     The "heads" are the aldehydes and esters which are drawn
off the top of the second distilling column, the aldehyde column.
The fusel oils and amyl oils are separated in a third column,
the rectifying column.  To recover these streams as fuel, they
are pumped from the"distil1ing columns to a storage tank and then
                 r
to a second burner installed in the plants' boiler.
                              IX-8

-------
              FERMENTERS
X
I
10
             L
              WASHOUT
               PUMP
                               BEER
                               DUMP
                                              CENTRIFUGE
                            f
SLUDGE
 PUMP
                          SOLIDS
                           TANK
             YEAST
             TANK
SURGE
TANK
                         SOLIDS TO
                         TRUCK FOR
                       LAND DISPOSAL
            YEAST  TO
            TRUCK  FOR
             SALE  OR
         LAND  DISPOSAL
         r8EER
          PUMP
                                                                                    TO
                                                                                    BEER
                                                                                    STILLS
                         JX-3.  Removal of fermenter bottoms and yeast  (80).

-------
     Both VIRIL and Puerto Rico Distillers already recover
fusel  oils and heads and burn them as fuel on a continuous
basis.   The heads and fusel oils would otherwise become part
of the  waste load requiring treatment.
COST
     Cost estimates from Puerto Rico Distillers and VIRIL (80)
for some of the options which have been discussed are presented
in Table  IX-1.  Estimates were available  for the removal of
fermenter bottoms, centrifugation of molasses,  yeast recycling
(without molasses pretreatment}, and burning of fusel oils and
heads.   Cost breakdowns for each are given in Appendix  IX-11
(80).   Except for the estimate for oil burning, none of these
estimates incorporate any cost savings due to by-product
recovery, or to positive impacts on rum production or waste
treatment.  Such savings could be considerable.
                              ix-io

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TABLE IX-1.  COST ESTIMATES FOR POLLUTANT REDUCTION THROUGH PROCESS MODIFICATIONS,
                    ...FROM PUERTO RICO DISTILLERS AND VIRFC*' (80)
                        Puerto Rico Distillers           VIRIL
 Process                    CapitalAnnualCapital    Annual

 Removal  of Fermenter      $50,000   $38,260        $58,000   $26,840
 Bottoms

 Yeast Recycling by       $200,000   $77,150       $232,000   $87,660
 Centrlfugation

 Molasses Centrifugation  $110,000   $59,585          N/At      N/A.

 Burning  of Fusel  Oils      $15,000     $0#         $11,500     $0
 and Heads
 ^Submitted January 30,  1978-
 tN/A = Not available.
 ^Assumed to be offset by fuel savings.
                                       IX-11

-------
            TABLE IX-2.  BREAKDOWN OF COST ESTIMATES FOR POLLUTANT
                   REDUCTION THROUGH PROCESS MODIFICATIONS,   *
                   . FROM PUERTO RICO DISTILLERS AND VIRIL.  (80)
REMOVAL OF FERMENTER BOTTOMS (VIRIL)

Capital Costs

Solids tank                             $15,000
Pumps, piping and electrical            $15,000
Structural                               $8,000
Dump truck                              $20,000
Total                                   $58,000

Annual Operating Costs

Depreciation                             $5,800
Maintenance                              $1,740
Labor                                   $12,000
Electricity                              $1,500
Financing                                $5,800
Total                                   $26,840

REMOVAL OF FERMENTER BOTTOMS (PUERTO RICO DISTILLERS)

Capital Costs

Pit tank                                 $2,000
Slurry pump                              $3,000
Thickeners                              $10,000
Water storage tank                       $5,000
Water pump                               $1,500
Piping, rearrangment of fermenters, etc., $3,500
Dump truck                              $20,000
Electrical installation, etc.            $2,000
Contingencies                            $3,000
Total                                   $50,000

Annual Operating Costs .(Direct)

Transportation and dumping of decanted
slurry                                  $14.400
Subtotal                                $14,400
 *Submitted January 30, 1978.
                                 IX-12

-------
Table  IX-2  (.continued)
 Annual  Operating  Costs  (Indirect)

 Repairs and maintenance                  $2,500
 Property tax                             $1,200
 Truck license                              $400
 Depreciation
   Truck                                  $4,000
   Other equipment                        $3,000
 Insurance                                  $500
 Payroll benefits  (402 in Statutory
 and corporate benefits)                  $5,760
 Financing                                $4,500
 Contingencies                            $2,000
 Subtotal                                $23,860

 Total                                   $38,260

 CENTRIFUGATION OF FERMENTER BEER (PUERTO RICO DISTILLERS)

 Capital Costs

 Centrifuges (2)                        $120,000
 Centrifuged mash  receiving tank         $18,000
 Centrifuge feed pumps (2)                $6,000
 Yeast cream receiving tanks (2)          $5,000
 Yeast cream pump                          $3,000
 Yeast washing tank                       $3,000
 pH controller                            $4,000
 Piping, valves, electrical, installa-
 tion, etc.                              $10,000
 Engineering consulting fees             $25,000*
 Contingencies                            $6,000
 Total                                  $200,000

 Annual Operating  Costs (Direct)

 Labor (1 laborer)                        $8,280
 Chemicals                                $4,000
 Electric power                           $S,QQQ
 Subtotal                                $17,280

 Annual Operating  Costs (Indirect)

 Repairs and Maintenance"                  $5,000
 Property Tax         '*"                   $7,860
 Depreciation                            $20,000
 Insurance                                  $700
 Payroll Benefits  (40% in statutory
 and corporate benefits)                  $3,310
                                        IX-13

-------
Table IX-2 (continued)
Financing                               $18,000
Contingencies                            $5,000
Subtotal                                559,870

Total                                   $77,150

*For study of yeast recycling following centrifugation.

CENTRIFUGATION OF FERMENTER BEER (VIRIL)

Capital Costs

Centrifuges  (2) (Sharpies Model P-3400) $160,000
Electric generators                     $50,000
Pumps,  piping and electrical            $TO,000
Structural                              $12,000
Total                                  $232,000

Annual  Operating Costs

Depreciation                            $23,200
Maintenance                              $4,860
Labor                                    $6,000
Electricity                             $30,400
Financing                               $23,200
Total                                   $87,660

CENTRIFUGATION OF MOLASSES (PUERTO RICO DISTILLERS)

Capital Costs

Molasses diluting                        $5,000
Mixer,  in line                           $5,000
Centrifuge                              $60,000
Solids  recovery tank                 '    $2,000
Centrifuged molasses tank                $5,000
Pump for 60° Brix molasses               $3,000
Piping, valves, eletrical installation,
etc.               •                      $4,000
Dump truck                              $20,000
Slurry  pump                              $3,000
Contingencies                            $3,000
Total                                  $110,000

Annual  Operating Costs (Direct)

Transportation and dumping slurry       $14,400
Electric power                         .  $3,000
Subtotal                                $17,400
                                      IX-14

-------
Table IX-2 (continued)
Annual Operating Costs (Indirect)

Repairs and Maintenance                  $3,000
Property tax                             $4,325
Depreciation
  Truck                                  $4,000
  Other equipment                        $9,000
Insurance                                  $800
Truck license                              $400
Payroll Benefits (40% in statutory
and corporate benefits)                  $5,760
Financing                                $9,900
Contingencies                            S5,000
Subtotal                                $42,185

Total                                   $59,585

BURNING OF FUSEL OILS AND HEADS (PUERTO RICO DISTILLERS)

Capital Costs

Storage tank                             $5,000
Pump                                     $1,500
Burner                                   $3,500
Piping and installation                  $3,000
Other                                    $2,000
Total                            •       $15,000

BURNING OF FUSEL OILS AND HEADS (VIRIL)

Capital Costs
Storage tank
Burner
Pump and piping
Total
$5,000
$2,500
$4?000
$11,500
                                    IX-15

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                        X.  AQUACULTURE

INTRODUCTION
     The fermentation and distillation of rum from cane molasses
produces relatively large quantities of hot acidic wastes, high
in BOO, COO, solids, and Kjeldahl nitrogen (80)..  Presently, these
wastes are disposed of untreated to inshore waters off Puerto
Rico and St. Crorx, Virgin Islands.  In an effort to develop a
satisfactory solutf'on to the problem of rum slops disposal,
the rum distillers have given more attention to evaluating aqua-
culture as a method for utilizing these .wastes.  Aquaculture has
the advantages of requiring  little or no pretreatment of the
effluent, considerable potential for recycling nutrients from
the waste into a harvestable crop, and providing acceptable levels
of predischarge treatment.
     In concept, the advantages of aquaculture are numerous;
however, several obstacles stand between  the concept and its
practical application.  Currently, there  is limited knowledge  .
of the manner in which rum slops can best be used in an aquacul-
                                                                i
ture project, and what species or chain of species leading to a •£.
harvestable crop ca-n utilize the slops efficiently.  Questions
as to the physical facility requirements, whether these facilities
can be located in reasonable proximity to the rum distilleries,
and what the projected capital and operating costs would be are
still unanswered.
                                X-V

-------
PREVIOUS RESEARCH IN MOSTOS UTILIZATION B,Y AQUATIC ORGANISMS
     The industry has solicited proposals for research into mostos
utilization in aquaculture in an effort to answer these questions.
A preliminary proposal for a feasibility study was received from
Or.  Colin E. Nash, Oceanic Institute, Makapuu Point, Waimanalo,
Hawaii (80).  A draft proposal was also solicited and received
from Or. Oswald A. Roels, University of Texas Marine Science
Institute, Port Aransas Marine Laboratory (80).  Dr.  Roels has
previous experience with an aquaculture project on St. Croix,
although this earlier study did not involve mostos.   Dr.  Roels
has  proposed to analyze the nutritive value of slops which will,
in turn, aid in the selection of  an  organism  or  organisms  suitable
for  treating and recycling each component of the waste.  Selec-
tion of suitable organisms will be based on the following cri-
teria:
     •  Potential for removal of dissolved inorganic nitrogen
        and/or phosphate
     •  Removal of particulate protein and carbohydrate
     •  Removal of soluble protein and carbohydrate  through cul-
        turing of unicellular heterotrophs.  These,  in turn,
        may be harvested and used directly as a protein source
        or fed to a higher trophic level  aquatic organism.
     Determination of tolerance levels to various concentrations
                   /*
of mostos and the^ability of test organisms to grow  and provide
satisfactory waste treatment will also be investigated.  Finally,
Or.  Roels''s  study  wou'ld  attempt  to  provide  preliminary  data  on  the
input requirements and potential yields of aquaculture systems.
                               X-2

-------
     The University of Puerto Rico is actively involved  in aqua-
culture research.  The principal objective of their studies has
been to discover additional sources of protein-rich foods for -
human consumption in Puerto Rico.  Most of the current research
concerns the growth of the fresh-water fish, Tilapia aurea. Only
one study utilizing mostos wastes has been conducted.  This study
investigated the affects of mostos on Tllapia aurea under culture
conditions and on the water quality of the culture system (80).
     A method for efficiently breeding and culturing fish, shell-
fish, and zooplankton with waste cane molasses has been  developed
by Kawano,. et al (53).  Applicability of this research to potential
mostos aquaculture experiments, however, is questionable due to
an inadequate description of the waste molasses used in  Kawano,
et al,. studies.  Characteristics of the waste molasses and its
similarities- to mostos are not provided in the patent information
filed by the authors  (53).       •
     Tosteson, et al (107) have conducted numerous  studies with
mostos to determine its effect on marine organisms.  Their work
examined four components of mostos:
     •  Filtered crude mostos suspension (Fraction FT)
     •  Crude mostos dialyzed against distilled water (Fraction '
        F2)
                                                                . ;5t
     •  Precipitate formed by the addition of three volumes of
                   i*
        cold ethyl alcohol to crude mostos fraction (Fraction F3)
     •  Fraction of crude mostos soluble in cold  ethyl alcohol
        (Fraction F4).
                              X-3

-------
     In their experiments, they used cultures of the marine algae
Chlorella vulgar is, and isolated the marine bacterium Photobac-
terium fi scher from sea water.   Their results indicated the fol-
lowing:
     •  Fraction Fl appeared to promote the growth of marine
        bacteria while inhibiting that of micro-algal cells. The
        crude mostos fraction may have inhibited the algal  growth
        by acting directly on the cells or by simply promoting
        the growth of bacteria  in the algal suspensions.
     •  Nondialyzable high molecular weight components of Fraction
        2 promoted the growth of algae and inhibited bacterial
        growth.   This suggested that the alcohol-treated material
        (Fractions 3 and 4) promoted both the growth of the algae
        and the  bacteria.
     r  The inhibitory effect of Fraction Fl  on algal growth.
        appeared to be due to the presence of low molecular weight
        components which were removed by dialysis.  These dialy-
        zable materials at high concentrations appeared to  stimu-
        late bacterial growth while obscuring the inhibitory
        effects  of high molecular weight material.
     •  The crude mostos fraction (Fl) contained materials  of.
        high molecular weight that were stimulators to micro-
        algal growth; however,  the presence of dialyzable low
                   <•
        molecular^weight substances in this same fraction promoted
        greater  bacterial  growth which, in turn, restricted or
        inhibited algal growth.
                              X-4

-------
POTENTIAL FOR UTILIZATION OF NUTRIENTS
     SCS Engineers contacted two noted authorities in aquacul-
ture. research for information on the potential  for utilization of
mostos in crustacean and higher organism aquaculture systems; Or.
Douglas Conklin, Associate Director of the  Aquaculture Program,
Bodega Marine Laboratry of the University of California, and
Dr.  Michael  Hartman, Division of Natural  Sciences, Savanah State
College, Georgia.  Copies of their correspondence are included
in Appendix  A.  Both agreed that the mostos material  was not
adaptable as a feed ingredient in  aquaculture systems.   Their
evaluation was based on several factors including the following:
     • • The  800 and COO are very high indicating that the mostos
        is very high in organic carbon compounds.
     •  High carbon content is further substantiated by high car-
        bohydrate content.
     •  Presence of significant amounts of  carbon compounds will
        enhance growth of heterotrophic bacteria.  These bacteria
        can  create situations of lowered  dissolved oxygen, as
        well as destroy the bacterial  flora of  a biological  fil-
        ter  system,should a system of this  type be in use as
        part of the treatment process.
     •  Levels of copper in mostos (average 32.8 ppm) are toxic  \
        to most sheJlfish and acutely toxic to  the larval stages.
                 i»
     •  Potential^ for bioaccumulation of  copper and other metals
        by aquatic organisms may be considerable considering con-
        centrations available.
                              X-5

-------
     Although additional  research is needed in this area, the
information currently available in the literature and from indi
viduals active in this research field suggests that mostos is
unsuitable for aquaculture systems.
                              X-6

-------
                 XI.   BIOLOGICAL TREATMENT SYSTEMS  .
INTRODUCTION
     Biological treatment of rum slops has long been a proposed
solution to the rum waste disposal problem (11).  However, the
consistently high 800^ has-'not favored aerobic treatment, and the
effluent from anaerobic treatment has been demonstrated to be high-
in B005 (2,000 to 10,000 mg/i).  Consequently, research has been
directed at the use of combinations of biological  systems (mainly
anaerobic followed by aerobic processes) for the treatment of rum
slops-.
AEROBIC TREATMENT OF MOSTOS
     A review  of the literature,  indicates that the  application
 of  conventional  aerobic  processes  (activated sludge or trickling
 filters)  is beset by problems.   In  general,  the  literature  reports
 that to be  amenable  to  aerobic  treatment the raw  mostos  would
 first  have  to  be  cooled  to  a temperature range conducive to  meso-
 phiMic organisms (25 to  35°C),  neutralized  to pH 7.0-8.0,  and
 have  supplemental nutrients added  (phosphorus  and/or nitrogen).
     Sen,  et al .  (92), reported  that  trickling  filter treatment
 of  undiluted molasses distillery waste  was found  to remove  75
                  /*
 percent of  the BOO",-.  A  large  filter area was  needed due to
 the high concentration  of 8005  in  undiluted  slops (18,000 to
 50,000 mg/2.) and  the low  800g  loading rate that  can be applied

                               XI-1

-------
to the filter.  The process was dismissed as inapplicable  because
of the space required by the filters.
     Burnett (19) reported that the activated sludge  process  was
unsati sfactory -when used with a 10 percent solution  of  rum  slops
in domestic sewage.  The maximum BODr removal observed  was  28  per-
cent.  Also, difficulties were caused by extensive foaming.  How-
ever, the investigator found that the activated sludge  process
was highly efficient for the treatment of one percent slops  in
domestic sewage.  Over 90 percent efficiency in BQOj. and  suspended
solids removal was observed.
     Kujala, et al . (61), estimated that in 1972 the  cost  for oper-
ating an activated sludge treatment plant for an  incoming  slops
stream of 35,000 mg/4 8005 at a flow of 1,000 m3/day (0.26  MGD)
would exceed $2.5 million per annum. Space requirements for  the
installation and  the electrical loads for the necessary aeration
equipment were  estimated to  be large.  Consequently, the  authors
rejected activated sludge treatment of the raw  slops on economic
grounds.
ANAEROBIC TREATMENT OF MOSTOS
      Anaerobic  digestion has been widely investigated as  a  method
for  the  treatment  of molasses distilling mostos,  and has  been
found to be a partially effective  treatment  alternative.  Anaerob'ic
                                                                 •v
digestion has the ability to handle  the  raw  undiluted slops  without
the  problems associated with aerobic  biological  treatment,  e.g.,
sludge  bulking.  Various designed  and  operational  methods  for
anaerobic digestion of mostos hava been  researched.   The findings
will  be  presented on the following pages.
                             .  XI-2

-------
     Sen, et al ., conducted  experiments  on  laboratory  scale
anaerobic digestion of molasses distillery mostos 'in India (92).
They found that it was possible to carry out anaerobic digestion
of the waste at 37 C using a cow manure seed at a BODe loading
of 3.0 kg/m /day with a retention period of 10 days.  The diges-
tion gave an off-gas to waste volume ratio of 25:1, with an
overall efficiency of 0.86 m  of gas per kg of S00g digested.
The gas was 60 percent methane.  SOOj removals of more than
90 percent were obtained with a BOD5 reduction from about
30,000 mg/2, to less, than 1,000 mg/i.  Digester failure was exper-
ienced at a loading of 3.8 kg/m /day with a. retention  time of
eight days.  Nutrient addition was found to be unnecessary.
Recirculation of CO^ after separation from other constituents of
the digester gas was more effective than mechanical stirring  or
recircul atio-n of the entire digester gas in improving BOOg and
volatile  solids reduction and the percentage of methane  in the gas.
     Shea, et al., in a study for the EPA investigated an anaerobic
digestion pilot plant for the determination of design  criteria for
the full-scale application of anaerobic treatment and  to estimate
the full-scale costs for such a process (93).  The pilot  system '!
gave a COD removal efficiency of 57 to 70 percent at a solids
retention time greater than 40 days.  The influent mostos stream;
contained 70,000 to 100,000 mq/i.  The range of total  annual
                   i*
costs  (including amortization and operating and maintenance  costs)
was estimated to vary from $3.74/m  treated at a design flow  of
190 m3/day to $2.13/m3 treated at a design flow of 1,140 m3/day
(January  1974 cost levels).  The recovery of methane gas as  an
                               XI-3 :

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energy by-product of the anaerobic treatment was estimated to
reduce the above-mentioned unit treatment costs from one-third
at the 190-m3/day flow rate to two-thirds at the 1 ,140-m3/day.
flow.   It was also concluded that additional treatment would be
required to meet the proposed effluent quality levels as defined
in the distillery's respective NPOES permits.
     Basu and Leclerc compared the treatment of beet molasses
distillery mostos by thermophilic and mesophilic anaerobic
digestion (9).   This research determined that gas  production
was less consistent in conventional  digestion when  compared to
units  with the thermophilic or mesophilic digestion.  This was
attributed to the loss of gas during settling and entrained in
effluent solutions.  Digester loading had no direct bearing on
gas production,  and instead was related to retention time.  The
efficiency of rate of methane production during high rate
digestion was related to C02 recirculation,  as shown by Sen and
Bhaskaran (92),  or to efficient mechanical stirring.
     All the experiments were done with 10 days retention time
at varying BOD loadings of 2.0, 2.5, 3.2, and 3.5 kg/m /day.
The thermophilic digestion BOD removal for the first three loadings
were 97.2, 95.7, and 87.5 percent respectively, as  against
corresponding removals of 96.0, 96.4, and 95.9 percent BOO      >
removal for mesophilic digestion.  Both thermophilic and-meso-
                   s-
philic digestion exhibited digester upset at the 3.5 kg 80D/m /day
loading.  Basu and Leclerc concluded that, taking all aspects
into consideration, thermophilic digestion performs as well as
                               XI-4 ,

-------
mesophilic digestion, but the process yields only minor benefits
at the expense of higher costs needed to maintain the digester
at 55°C rather than 35°C  (9) .
     Hiatt, et al. , found that the. start-up and initial acclimati
zation period was very significant in the anaerobic treatment
of mostos  (4>8)-r  They determined  that nearly  three months
were required for the biological  system to acclimatize itself
to a new substrate.  After stabi1ization, a change in the loading
rate required, 10 to 15 days, for a return to normal operating
conditions.  Dilution varied the COD loading rate by between
2.7 to 11.5 kg/m
        performance and stability and adds valuable nutrients.  *
     •  The anaero-b'ic digester for optimum performance must be
                 i-
        fed an equalized waste stream.
                              XI-5

-------
     •   Recirculating  C0?  qas  (scrubbed  to  remove H-S)  and/or
        mechanical  agitation  of the  digester contents  improves
        the  organic  matter removal  efficiency of  the  digester
        and  can  lower  the  retention  time necessary for  treatment.
     «   The  retention  time for anaerobic digestion could  be as
        short  as  5.6 days, although  normal  performance-  requires
        about  10  days.
     •   Volatile  acid  production  must  be controlled by  either
        chemical  addition  or  by dilution with other wastewater.
     t   Gas  production  is  approximately  55  to 65  percent  methane
        and  45  to  55 percent  carbon  dioxide.
     •   The  effluent from  anaerobic  digesters treating  rum
        distillery  mostos  must have  some form of  additional
        treatment  to meet   the  proposed  NPOES  limitations.
ANAEROBIC/AEROBIC SYSTEMS
     Cosculluela,  in Cuba, investigated  a biological  system for
the treatment  of molasses  distillery waste  (11).   His  studies
ran for a period of three  years.   Cosculluel'a recommended that
mostos  be diluted four times  with water, mixed with a  bacterial
culture rich in ammonifying organisms, and  then digested  in an
open concrete  tank for a period of four  days.  He suggested that
the effluent then be passed through biological filters  for addi- ,
tionalstability.    ^
     Recent research in Russia and Poland has indicated the feasi-
bility of biological systems  for the treatment of molasses ferment'
ing and distilling wastes.  In Russia, stillage was treated by
                               XI-6

-------
anaerobic fermentation which reduced the initial BOOT of greater
than 10 g/i by 90 percent.  This anaerobic effluent was adjusted
to SODy 800 to 1,300 mg/ii by dilution with municipal wastewater
and treated by activated sludge to give an effluent of 800^ 24- to
60 mg/i, and COD 180 to 240 mg/z (1H) .   In Poland, spent molasses
from feed yeast production with a SOOg  of 12,870 mg/z and a pH
of 4.4 were anaerobical1y fermented and then treated by the acti-
vated sludge process.  Without phosphate nutrient addition the
effluent from the system had a BODg of  70 to 600 mg/z; with phos-
phate addition the effluent BODg concentration was 75 to 300 mg/z
(105).
     Bhaskaran (10)  reported that laboratory-scale anaerobic digeS'
tion reduced the 800= of molasses distillery slops from about
30,000 mg/4 to the order of 1,000 mg/z.  The 800-to-nitrogen ratio
of the digested slops effluent indicated that it was suitable for
activated sludge treatment.  In the laboratory, activated sludge
was built u.p by aerating sewage for a number of days.  The activa-
ted sludge was then gradually acclimatized to the digester efflu-
ent.  When stable conditions of operation were established, the
results showed that aeration for 8 hr effected 93 percent 8005
removal and yielded an effluent with 63 mg/z 3005-
THE ANAMET PROCESS  '                                            "X
                                                                 >
     A proprietary ,jriol ogical system has been developed in Sweden
incorporating anaerobic and aerobic treatment.  This process, the
                               xr-7

-------
Anamet process, differs from other biological systems in that it
has all  of the following characteristics:
     1.   Use of a closed tank for the anaerobic stage rather than
         an open pond.
     2.   Recovery of methane gas for reuse as fuel.
     3.   Recycle of digester sludge within the anaerobic process
         after separation from the digester effluent.
     4.   Recycle of waste-activated sludge to the digester.
     5.   Optional nitrogen stripping with recovery of ammonium
         salts.
     Other biological systems may have  some  of  these  characteris-
tics, but Anamet necessarily has all of them.
Applicability to Rum Wastes
     The rights to the Anamet process are held by the Swedish
engineering firm of AB Sorigona, located in Staffanstorp, Sweden.
Anamet was developed especially to handle wastes from fermentation
and food industries, rich in carbohydrates and nitrogen compounds.
Anamet has not been used to treat rum distillery wastes, but it
is in full-scale operation at four beet sugar refineries and one
potato alcohol distillery in Sweden.  The process has also been
in use for approximately two months on a pilot plant basis at a
beet molasses alcohol distillery in Belgium (.99).  According to
representatives of Sorigona, the results of existing Anamet plants
have been excellent, with BOO  reductions of up to 99 percent being
achieved.  After visiting the facilities of Puerto -ftico Distillers
                               XI-8

-------
 and  laboratory  testing  of  the  mostos,  Sorigona's  representatives
 expressed  the  belief  that  mostos  could  be  treated successfully
 with  Anamet  (49).   Subsequently,  a  pilot  plant was constructed.
 and  began  operating  in  July  of 1978.   The  plant has  a  rated capa-
 city  of  about  6,000  A/day,  or  0.7 percent  of  the  total  waste flow.
 Initial  tests  were-begun with  a  25-percent-by-volume digester seed
 of digested  municipal  sludge.  Preliminary results are  not  yet
 available  from  this  operation.
 £quipment  and  Design
      The equipment, used and the design of  Anamet  plants vary with
 different  wastewaters  and. with the  required  final BOD  (99). Figure
 XI-1   presents a typical  Anamet  system.   The anaerobic step  is- car-
 ried out in  a  steel  tank.   The ta.nk has a  side-mounted stirrer for
 mixing and to  keep the  sludge  suspended.   It  also has  a sedimen-
 tation zone- in which sludge is separated  from the wastewater and
 retained.   From the top of the tank,  methane  gas  is  drawn off to
 a gas supply.   The pH  and  temperature  are  optimized  for the anaero-
 bic  conversion of the  wastes into methane  gas.
      The aerobic step  is  carried out  as an activated sludge pro-
. cess in  a  concrete basin  or pond.  Aeration  is performed by sur-
 face aerators  or in cold  climates by  submerged aerators.
      Sedimentation of aerobic  sludge  and  final clarification of ^
                                                                 ••*>
 the  wastewater are .carried out in a horizontal thickener with rakes
                   /•
                  M
 or in other  suitable equipment.  Waste sludge is  pumped to  the
 anaerobic  tank for stabilization-.
                               XI-9

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               ANAEROBIC
               DIGESTER
                                        CLARIFIES
                                  EFFLUENT
               WASTE
               SLUDGE TO
               DIGESTER
DIGESTER--
AGITATOR
     Figure 'XI-1. Typical  Anamet  System
                    XI-10

-------
     Where nitrogen reduction is involved, it is achieved by strip
ping the water with air after the anaerobic step.  Most of the
organic nitrogen compounds have by then been decomposed and the
nitrogen remains as ammonium ions.  Lime  is added in order to
increase the pH of the solution and this  frees the ammonia before
stripping.  The ammonia is absorbed and a concentrated ammonium
salt solution is produced which permits recovery of ammonia for
reuse as a. nutrient in the fermentation process.
Process Performance
     Tha specific  improvements  in performance which Anamet systems
attempt to provide over other anaerobic/aerobic  systems include:
     •  Low overall sludge generation
     t  Short digester retention time
     *  High methane gas production
     •  Excellent  800 removal.
     These features are discussed below.
     In the Anamet process the  sludge created in the aerobic stage
is  recycled into the anaerobic  stage and  converted into methane
and carbon dioxide, thus reducing  to a  minimum  the amount  of
solid waste which  would have to be disposed of upon completion of
the process.  A Swedish sugar factory using the Anamet process   ;
reported no surplus sludge generated for  the first three months  '\.
                                                                  "*•»
after start-up of  the. full-scale plant  (99).
                    /•
     The hydraulic retention time for the wastewaters in the anaer
obic step may vary between two  and 15 days, depending upon the
nature of the wastewater.  In order to   avoid extensive digester
                              XI-11

-------
retention times, which would be a problem in the treatment of rum


distillery wastes, the anaerobic sludge is retained in the system.


This is usually achieved by separation in a settler and recircula-


tion of the thickened sludge.  The accelerated activity in the


anaerobic step is also facilitated by the return of the biosludge


from the aerobic step.  This sludge acts as a "support" for the


enzymes of the anaerobic bacteria (99).


     The operating conditions of the system are maintained so as

to maximize the anaerobic conversion of the waste into methane gas


The amount of gas produced depends on the wastewater and is repor-


ted to vary from 0.3 to 0.5 m  CH./kg BOD, digested.  Sorigona


suggests that the digester operating parameters as  shown in


Table XI-1 be maintained to optimize the conditions for the


methane-forming bacteria (49).




          TABLE XT-! .  .RE.CQMMEMDEQ' OPERATING. PARAMETERS


        FOR ANAEROBIC DIGESTION IN THE ANAMET SYSTEM (49)

              Parameter         Typical Operating Ranoe


     pH                              6.8 - 7.5


     Temperature                       35 - 60°C


     Bacterial retention time          >6 days


     Wastewater retention time           2-20 days


      Process  toxicity levels         NH. <1,000 mg/i
                    r                  "

                                     K+, Na +  < 5,000  mg/z

                                     H2S  <  100 mg/L
                               XI-12

-------
     Wastewaters with high inorganic salt concentrations,  such  as
mostos, may tend to increase the minimum bacterial retention  time
shown in Table XI-1. In addition, there, are toxic effects from
high concentrations of inorganic compounds such, as ammonium,  potas-
sium, sodiuum, and hydrogen sulfide in the wastewater.  By adapt-
ing the bacteria, however, it may be possible to operate at con-
centrations higher- than the normal  toxic levels shown  in Table
•XI-1  C49],
     BOD reductions with Anamet vary depending upon the specific
wastewater and. the retention time,  but Sorigona claims that BOD
 reductions  of  up  to  99  percent  have been experienced  in  the  treat-
 ment  of  wastes  other  than  rum distillery effluents.   Sorigona
 reports  that  in  various  situations  when  the  Anamet  process  has
 been  used,  between 70  and  90  percent  of  the  original  800 was
 removed  in  the  anaerobic  step,  leaving  10  to  30 percent  to  be
 treated  in  the  aerob.ic  step.  This  high  degree  of  800  reduction
 in  the  anaerobic  step  is  primarily  attributable to  the production
 of  methane  gas.   Methane  has  a  low  water solubility  and, conse-
 quently,  nearly  all  of  it  escapes  from  solution,  thereby signifi-
 cantly  reducing  BOO  (99).
      The  BOO  reduction  which  has  been reported  for  the second step
 of  the  Anamet  process  is  very high, with up  to  approximately  93 >
'percent  reduction reported for  some wastes.   The  efficiency  of  the
 300 reduction  in  tire  aerobic  step  is  attributed to  the anaerobic
 pretreatment  where the  large  molecules  are  hydrolyzed  into  sub-
 stances  which  are easily  assimilated.   Moreover,  a  sufficient

                                XI-13

-------
amount of inorganic nitrogen remains in the water to facilitate
the conversion of the biomass in the aerobic step (99),
     A complete list of Anamet facilities existing and under con-
struction is contained in Appendix  B.  it includes the type of
waste treated and the BOD5 reduction achieved at each plant, as
reported by Sorigona (50).  The types of wastes which have been
treated with Anamet generally have had BOOr and COO concentrations
considerably lower than those of Caribbean mostos (49,95,99). BOOg
concentrations have been  in the range of 4,000  to 13,000 mg/2, for
many of the wastes treated, while mostos has a  typical  BOOg concen-
tration of about 35,000 mg/i.  Therefore the results which will
become available  from Puerto Rico Distillers'  pilot Anamet facility
at  Arecibo  are of great  importance  in determining the adaptability
of  the process to mostos.
 Costs
      The  pilot plant results are also critical  to determing  the
costs  of  a  full-scale operation.  According to  Sorigona  (99),  the
investment  costs  for an  Anamet  plant-are  generally  low,  on the
order  of  $50  to  $100/kg  of  BOD,  removed  per day,  due to  the  compact-
ness  of  the plant.  Operating costs  are  reportedly negligible  since
no  nutrients  are  needed,  and energy  as  well as  personnel  require-
ments  are  low.   Sludge handling  costs are also  small.   The value'..
of  methane  gas produced  is  said  to  more  than cover  the  operating
                  i"
costs  for  many applications  (99).
                              XI-14

-------
     A8 Sorigona has provided Puerto Rico Distillers with a turn-
key investment price estimate for a full-scale facility (ad). The
estimate is for a loading rate of 65,000 Ib of 800/day, 225,000  gal
of mostos/day, and assuming' 95 percent 3005 reduction.  The turnkey
investment cost estimate submitted April 4, 1978,  in U.S. dollars
was $2.55  x 10  . This  is -a  cost of approximately $91/kg of BOO-
removed per day. Methane gas production was estimated at the equi-
valent of  Z,340 tons of fuel oil per year. Electrical power con-
sumption was estimated at 1 x 10  kWh/yr; and sludge, production
at 6,700 m /yr. The cost of sludge transport was calculated at
$57ton, but this does  not include land (EQ).
     One. of the relative  disadvantages  of  any  of  the anaerobic/
aerobic biological  treatment systems  is that  the  effluent  remains
very dark  in color.  It would be technologically possible to
remove the color from  the effluent by means of large quantities
of activated carbon or by ozone oxidation.  Either method would
be prohibitively expensive, however (24).
                               XI-15

-------
CULTIVATION OF FODDER YEASTS, BACTERIA, AND FUNGI IN SLOPS
     The propagation of single strains of various yeasts, bacteria,
and fungi in waste liquids is sometimes referred to as single-cell
protein synthesis.  Since these organisms can metabolize the organic
materials of a substrate which itself conta.ins relatively little
protein, the high protein content of the biomass represents a trans-
formation of the nitrogen of the substrate to form a protein bio-
synthesis.   Previous investigations utilizing rum slops as a sub-
strate have been performed primarily with certain yeasts and molds
(considered fungi).   When extracted and dried, the yeast or mold
biomass (containing  approximatley 40 to 46 percent protein) has a
relatively high market value as an animal feed supplement (52).
     A flow sheet for yeast manufacturing from molasses alcohol
stillage, as it was  performed at Hsinying By-Product Factory (Zl),
is shown in Figure XI-Z,. Preceding the fermentation of the stillage,
yeast inoculum was prepared using a shaking cultivation and two
seed tanks.  Molasses was employed for the culture to maintain
strong and active yeast.  The stillage coming from the distillery
was cooled, but neither dilution nor sterilization was required.
Stillage was continuously fed to a fermentation tank by displace-
ment.  Supplemental  nitrogen and phosphorus were provided by the
addition of a cream solution of urea, ammonium sulfate, and calcium
superphosphate.  Airr was provided with a turbo blower.  The tempera-
ture was regulate'cl by a refrigeration cooling system designed with
a heat exchanger to  eliminate the possibility of contamination of
the fermenter.  The fermented stillage flowed from the tank to a
                               XI -1 6

-------
        YEAST
        SEED
SHAKING
CULTIVATION
WASTE
LIQUOR
x
i—i
i
—i
       STILLAGE
                NUTRIENT
                SOLUTION
                FEED TANK
  COOLING
  TANK
                                                                  SEPARATORS
                                                       FERMENTATION
                                                       TANK
                 AIR
                 BLOWER
                                                                 HEAT
                                                                 EXCHANGE
                                            TURBO
                                            COMPRESSOR
                                            REFRIGERATING
                                            MACHINE
             HAMMER
             MILL
                                                                                DRIED
                                                                                YEAST
                                                                                PRODUCT
                                                              HEAT
                                                              EXHAUST
                                                                     REF.
               Figure  XI-2,  Yeast Cultivation  in Molasses  Distillery  Stillage*

-------
yeast separator.  The separated yeast went through a drum dryer
and hammer mill to form the final product.
     Yeast cultivation of molasses alcohol stillage has been inves-
tigated in Taiwan (21, 64).  Successful work has been performed
with Torula uti1is, which has a protein content of 46 percent.  One
ton of dry torula yeast was produced from 60 to 65 tons of stillage
in a small, factory-scale aerobic fermenter.  In spite of these
excellent results, it is expected that in most cases up to 100 tons
or more of stillage may be required to yield one ton of dry torula
feed yeast (47), mainfy due to the high BODr of the Taiwanese
stillag-e (approximately 60,000 ppm) .   Stillage BODr values from
Puerto- Rican rum processors,  in contrast, have been shown to be
lower (average total  BOD values were reported to be 32,900 and
54,900 ppm for 6 and  5 samples, respectively) (93).
     Researchers in Taiwan (21) have, reported that the carbon source
for yeast growth is derived principally from the organic matter por-
tion of the stillage.  In laboratory and  factory fermentations, the
pH  increased rapidly from 6.8  to  7.9 during yeast  formation, indi-
cating that much of the energy being used was from volatile acids,
lactic acid, and other combined organic acids in the stillage.  Stil-
lages from beet and cane molasses are different in organic composi-
tion:  the relative ash content of cane molasses is about 40 percent
higher than that of,-its beet counterpart  (96).  It is well known
that beet molasses provides a  better substrate  for yeast production
than cane molasses.  Therefore, it is expected that beet molasses
stillage will make a better substrate than cane molasses stillage.
                              XI-18

-------
     In the Taiwanese experiments (64) with Torn la utilis, the BOOg
concentration of the stillage was reduced by 33 percent, and the
total  solids concentration by 29 percent.  In Soviet experiments
(51) with de-yeasted beet molasses stillage, the cultivation of
various fodder yeasts decreased the 8005 by 41 to 54 percent, and
the total solids by approximately 37 percent.  The Soviet experiments
involved dilution of the stillage prior to yeast cultivation, as
have experiments carried out by French investigators (61).  These
latter experiments indicated that dilution promoted the yeast
activity.
     The amount of income to be derived from yeast production is
dependent upon the yeast yield and its marketability.  The produc-
tion costs may vary considerably, depending upon the extent to which
additional nutrients must be supplied for the process, the retention
times required, and the steps necessary to separate the yeast from
the liquid.  Research in some of these areas  (30) is underway at
Puerto Rico Distillers' laboratories  in Arecibo, Puerto Rico.  Exper-
iments have already been conducted using Aspergi11 us phoenici us
(a  fungus)  in fermentation tanks with up to 1,500-liter volumes.
The highest SOOg: reduction achieved in these  tests was 59 percent
at  a retention time of seven days.  Puerto Rico Distillers (80)
regards  a retention time of greater than two  to three days as exces-
sive to  be  useful  in-a  full-scale operation,  due to a lack of
                   ^*
space  and  the high  costs  for tanks.   These experiments also yielded
disappointing results  concerning  the  separation of  the yeast from
solution.   3ased on  earlier experiments  of a  smaller  seals at  the

-------
Rum Pilot Plant of the University of Puerto Rico, Puerto Rico
Rum Distillers had hoped for strong formation of a mycelium, a
visible ma'ss of microbial  cell  matter, which would easily be
removed from solution. However, mycelium formation in the experi-
ments was not as consistent as  had been expected.  Further infor-
mation in these areas, and on supplementary nutrient requirements
as well, is needed to make .production cost estimates.
     Another important cost consideration is electrical  power.  Power
is consumed primarily for aeration purposes, as many as  400 volumes
of air being required per volume 'of rum slops (61).   Power require-
ments for the operation of a cooling system may also be  considerable
since a large amount of heat is evolved during feed yeast cultiva-
tion, and must be continuously removed to safeguard fermentation
activity and maximize yield.
     After yeast cultivation and separation, the slops will still
pose a substantial treatment problem due to the remaining high B005
level.  Because much of the organic matter of the waste  will have
been used in producing yeast, the value of the waste in  the pro-
duction of a gas by anaerobic digestion will be reduced.  Its min-
eral content would be extremely high if evaporated to CMS for use
as an animal feedstuff and, if the CMS were incinerated, the amount
of heat recoverable for the needs of evaporation would be lower.'
On the positive side-, however,  the reduced strength of the waste
could improve the operation and performance of aerobic biological
treatment.
                               XI-20

-------
                           (  REVISED )
                       XII.  BIBLIOGRAPHY
r.
    Allen,  D.M.,  R.L.  Sargent,  and  J.A.  Obertreuffer.   Treatment
    of combined  sewer  overflows  by-high  gradient  separation.
    EPA/600/2-77/015,  Sala  Magnetics,  Inc.,  Cambridge,  Massachu-
    setts,  March  1977.   130p.~  (Available  from  National  Technical
    Information  Service  (NTIS)  as  P8-264 935).
    Ammerman, C.8., P.R. Henry, S.G. Potter,
    Effect of condensed molasses solubles as
    for cattle.   Presented at the Xlth International
    Nutrition, Rio Oe Janeiro, August 1978.
                                             and  H.M.  Seeker.
                                             a  feed  ingredient
                                                     Congress
                                             of
    APV Company, Inc.  APV processing equipment.
    Tonawanda, New York, 1978.  19p.
                                                  Bulletin  CP-475
4.  Bacardi Corporation.  Written testimony presented at .adjudi-
    catory hearings for Virgin Islands Rum Industries, Limited,
—  Puerto Rico Distillers and Schenley Industries,  Inc., 1978."
4a.
5.
    Backus,  A.A.
    1 ery slops.
 and C.  Haner,  Jr.   Volatile  acids  from  distil-
U.S.P.  1,396,006  and 1,500,131,  November 8,  1922
    Basora and Rodriguez,  Consulting  Engineers.   Facilities  plan-
    ning for the  Bayamon  W.astewater  Region.   Draft  environmental
    impact statement.   Prepared  for  Puerto  Rico  Aqueduct  and
    Sewer Authority,  Commonwealth  of  Puerto  Rico, March  1975.

5.   Basora and Rodriguez,  Consulting  Engineers.   Facilities  plan-
    ning for the  Bayamon  Wastewater  Region.   Vol.  II.  Engineering
    report.   Prepared  for Puerto  Rico  Aqueduct  and  Sewer  Author-
    ity, Commonwealth  of  Puerto  Rico,  June  1976.

7.   Basora and Rodriguez,  Consulting  Engineers.   Facilities  plan-
    ning for the  Bayamon  Wastewater  Region.   Vol.  IV.  Oceanogra-'
    phic study.   Prepared  for Puerto  Rico Aqueduct  and  Sewer
    Authority, Commonwealth  of Puerto  Rico,  March  1976.

8.   Basu, A.K.  Characteristics  of distillery wastewater.   J.
    Water Pollut.  Control  Fed.,  47:2184-2190, 1975.
                             XII-1

-------
 9.   Basu, A.K.,  and L.  leclerc.   Comparative  studies  on  treat-
     ment of beet molasses  distillery  waste  by thermophilic  and
     mesophilic digestion.   Water  Res.,  4:103-104,  1975".

10.   Bhaskaran, T.R.  Utilization  of materials derived  from  treat-
     ment of wastes  from molasses  distilleries.   Adv.  Water  Pol-
     lut. Res. , 2(2):85-104,  1964.

11.   Biaggi, N.  Studies on  rum distillery wastes  in  Puerto  Rico.
     Ph.D. dissertation.  University of  Michigan,  Ann  Arbor,  1956.

12.   Bolton, D.H.,  O.A.  Hines,  and  J.P.  Bouchard.   The  applica-
     tion of the  ICI Deep-Shaft Process  to industrial  effluents.
     Proc. Ind. Waste Conf.,  31:344-351,  1976.

13.   Boruff, C.S.  Recovery  of  fermentation  residues  as feeds.
     Ind. Eng.  Chem. , 39:602-607,  1947.

14.   Bradley, B.J.   ICI  Deep-Shaft  Effluent  Treatment  Process.
     Presented  at the 24th  Annual  Meeting Canada  Section,  Ameri-
     can Water  Works Association,  Halifax, Nova Scotia, September
     12-15, 1976.

15.   Srau, H.M.,  Puerto  Rico  Distilleries, Inc.   Supplemental
     testimony  before the United  States  Environmental  Protection
     Agency in  the  matter of  National  Pollutant Discharge  Elimir_
     n.ation System  Permits  for  Puerto  Rico Distillers,  Inc.,.
     Schenley Industries, Inc., and Virgin Islands  Rum  Industries,
     Ltd.

16.   Brooks, N.H.  Conceptual  design of  submarine  outfall.   I.
     Jet diffusion.   Technical  Memorandum 70-1, California  Insti-
     tute of Technology, Pasadena,  W.M.  Keck Laboratory,  1970.
     19p.

17.   Brooks, N.H.  Conceptual  design of  submarine  outfall.   II.
     Hydraulic  design.   Technical  Memorandum 70-2,  California
     Institute  of Technology,  Pasadena,  W.M. Keck  Laboratory,
     1970.  12p.

18.   Brooks, N.H.  Dispersion  in  hydrologic  and coastal environ-
     ments.  Report  No.  KH-R-29,  California  Institute  of  Techno-;
     logy, Pasadena, December  1972.  136p.

19.   Burnett, W. F.. '-Rum  distillery  wastes: laboratory  studies  on
     aerobic treatment.   Water  Sewage  Works, 120:107-111,  1973.
                              XII-2

-------
20.   Chakrabarty, R.N.  Potash recovery - a method of disposal  of
     distillery wastes and saving foreign exchange.   Presented  at
     Symposium in New Developments of Chemical  Industries Relat-
     ing to Ethyl Alcohol, Its By-Products and  Wastes,  New Delhi,
     October 14-16,  1963.

ZT .   Chang, C.T., and W.I. Yang..  Study of feed yeast production
     from molasses distillery still age..  Taiwan Sugar,  20:200-205,
     1973.

22.   Chatterjee, A.C.  Production of fertilizer from distillery
     spent wastes.  Presented at the 38th S.T.A.  of  India, Kan-
     pur, 1972..

23.   Conklin, O.E.,  Bodega Marine Laboratory,  University Of Cal-
     ifornia.  Personal  communication to M.A.  Caponigro, Septem-
     ber 22, 1978.

24.   Crowley, 8.A.,  Schenley Distilleries, Inc.  Supplemental
     testimony before the  United States Environmental Protection
     Agency for the  matter of National  Pollutant Discharge Elimi-
     nation Permits  for Puerto- Rico  Distillers, Inc., Schenley
     Industries, Inc., Virgin Islands Rum Industries, Ltd.

25.   Cruver, J.E., and J.  Nusbaum.  Application of reverse osmo-
     sis to wastewater treatment.  J. Water Pollut.  Control  Fe.dT",
     46:301-311, 1974.

2'6.   Gulp, R.L., and G.L.  Gulp.   Advanced Wastewater Treatment.
     Van Nostrand Reinhold, New York, 1971.  3l8p.

27.   Damron, 3.L.  Condensed molasses solubles  in poultry feed;
     final research  summary to George H. Oorion,  Bacardi Corp.,
     Jacksonville, Florida.

28.   Oedert Corporation.  Dependable process equipment.  Chicago
     Heights,  Illinois, 1978.  9p.

29.   Diaz-Medina, M. and P.F. Randel .  Dried rum distillery s'til-
     lage in laying rations.  J. Agric. Univ.  P.R.,  62:149-155,
     1978;

30.   Dorian, G., Bacardi Corporation,  Personal communication  to ••*•
     K.V. LaConde, July 18, 1978.
                 j-
31.   Dorian, G., Bacardi Corporation.  Personal communication  to
     K.V. LaConde and C.J. Schmidt,  July 20, 1978.

32.   Dorian, G., Bacardi Corporation.  Personal communication  to
     K.V. LaConde, September 1,  1978.
                              XII-3

-------
33.   Dorian G., Bacardi  Corporation.  Personal  communication to
     K.V. LaConde, September 28, 1978.

34.   Dorian G., Bacardi  Corporation.  Submitted testimony before
     the United States Environmental Protection Agency in the
     matter of National  Pollutant Discharge Elimination Systems
     Permits for Puerto  Rico Distillers, Inc.,  Schenley Indus-
     tries, Inc., and Virgin Islands Rum Industries, Ltd.

35.   Edwards, J.Y., Pacific Molasses Company.   Supplemental  tes-
     timony before the Environmental Protection Agency in the
     matter of National  Pollutant Discharge Elimination System
     Permits for Puerto  Rico Distillers, Inc.,  Schenley Indus-
     tries, Inc., and Virgin Islands Rum Industries, Ltd.

36.   Environmental Science and Engineering.  Draft development
     document for effluent limitations and new  source performance
     'standards: miscel 1 arreous foods and beverages point source
     category.  Gainesville, Florida, March 1975.  5 vols.

37.   Falcman, W.  Combustion of molasses slops  as a solution of
     the wastewater 'problem in the alcohol  industry.  Szeszipar,
     19:103-105, 1971.

38.   Gabel , D.H., Lederle Laboratories.  Personal communication,
     March  1978.                                               —

3-9.   Gartner, R.J.W, and P.K. O'Rourke.  Effects of antibiotics,
     .dried  molasses distillers solubles and zeranol in all-sorg-
     hum srain rations fed to steers.  Aust.  J. Exp. Agric.  Anim.
     Husb. , 17:214-220,  1977.

40.   Gillies, M.T.  Animal Feeds from Waste Materials.  Noyes
     Data Corporation, Park Ridge, New Jersey,  1978.  346p.

41.   Gross, R.W., Jr.  High purity oxygen activated sludge pro-
     cess for industrial wastewater.  Union Carbide Corporation,
     South  Charleston,-West Virginia.

42.   Gyger, R.F., and E.L. Doerflein.  Save energy - use 02  on
     wastes.  Hydrocarbon Process., 55:96-100,  1976.

43.   Haner  C.  U.5.P.  1,430,160.

44.   Harada, Y., K.'"Hirano, and T. Koike.  Closed system in  fer-
     mentation was'tewater treatment.  Presented at Chemical  Engi-
     neering Congress, Denver, August 29, 1977.  5p.
                             XII-4

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45


46


47


48



49
50

51
Hartner, M.,  Savannah State College.   Personal  communication
to M.A.  Caponigro,  October 9,  1973.

Heres Gonzalez, A.   Personal  communication  to K.V.  LaConde,
September 22, 1978.

Heres Gonzalez, A.   Personal  communication  to K.V.  LaConde,
October 13, 1978.
Hiatt, W.C. :
tion of rum
28:966-976,
   A.O.  Carr,  and  J.F
  disti11ery wastes .
  1973.
                   Andrews.
                  Proc.  Ind.
                 Anaerobic diges-
                 Waste Conf.,
Huss, L.  Methane production from Cassava Distillery stillage
with the Sorigona WWTS.   Presented at the Semario  Interna-
cional  Sobre Tratamento  de Vinhoto,  Rio Oe Janeiro,  August
1976.  6p.                                  -

Huss, L., A.8.  Sorigona.   Personal  communication.   April  1978

Il'ina, L.O., N.G. Sitnik, E.I.  Podgurskaya,  M.S.  Pavlovskaya
and L.V. Rudnichenko.   An investigation of the chemical  com-
position of ololasses stillage with an evaluation  of  the
     degree
     30-34.
       of its purification.   Fermentn.  Spirt.  Prom.,. 1974
52
53
54
55


56
Jackson, C.J.  The treatment of distillery and antibiotics
wastes. . In: Waste Treatment; Proceedings of the Second Sym-
posium on the Treatment of Wastewaters,  1959.   Pergamon
Press, New York, 1960.  pp.  226-239.
Kawano ,  T.
and method
fi1ed June
,  H.
 of
 27,
 Kojima,  H
aqui animals
Ohosawa, and K.  Morinaga.   Feed
cultivation.  Patent 3",939,279,
                    1973, awarded February 17, 1976
Knezek, B.D., and R.H. Miller, eds.   Application of sludges
and wastewaters on agricultural  land; a planning and educa-
tional guide.  North Central  Regional Research Publication
235, Ohio Agricultural Research'and  Development Center,  Woo-
ster, October 1976.  98p.
Koh, R.C.Y., California
Personal Communication,
              Institute  of  Technology,  Pasadena.
              July  1978.
Koh, R.C.Y. ,  a-nd N.H. Brooks
disposal in tfhe ocean.  Annu
1975.
                     Fluid  mechanics  of
                    Rev.  Fluid  Mech.,  7
                                    wastewater
                                    187-211 ,
                              XII-5

-------
57.   Krabbe, E.,  U.S.  EPA Region II Facilities Technology Divi-
     sion.   Testimony  in rebuttal  to Bacardi  Corporation for the
     adjudicatory hearing in the matter of Puerto Rico Distillers,
     Inc.,  Schenley Industries, Inc.,  and Virgin Islands Rum
     Industries,  Ltd.

58.   Krabbe, E.,  U.S.  EPA Region II Facilities Technology Divi-
     sion.   Testimony  in rebuttal  to Schenley Industries, Inc.,
     and Virgin Islands Rum Industries, Ltd., for the adjudicatory
     hearing in the matter of Puerto Rico Distillers, Inc.,  Schen-
     ley Industries, Inc., and Virgin  Islands Rum Industries, Ltd.

5.9.   Krabbe, E.,  U.S.  EPA Region II Facilities Technology Divi-
     sion.   Testimony  in rebuttal  to Puerto Rico Distillers, Inc.
     for the adjudicatory hearing in the matter of Puerto Rico
     Distillers,  Inc., Schenley Industries, Inc., and Virgin
     Islands Rum  Industries', Ltd.

60.   Krabbe, E.,  U.S.  EPA Region II Facilities Technology Divi-
     sion.   Testimony  of Erik Krabbe for the  adjudicatory hearing
     in the matter of  Puerto Rico Distillers, Inc.,  Schenley
     Industries,  Inc., and Virgin Islands Rum Industries, Ltd.

61.   Kujala, P.,  R. Hull, F. Engstrom,  and E. Jackman.  Alcohol
     from molasses as  a possible fuel,  and the economics of  dis-
     tillery effluent  treatment.  Sugar Azucar, 1976:28-39.

62.   Kulperyer, R.J.,  and L.C. Matsch.   Comparison of treatment
     of problem wastewaters with air and high purity oxygen  .acti-
     vated  sludge systems.  Presented  at the  International  Asso-
     ciation of Water  Pollution Research on Design - Operation
     Interactions at Large Wastewater  Treatment Plants,  Vienna,
     September 8-12, 1975.

63.   LaConde, K.V., R.J. Lofy, and R.P. Stearns.  Municipal  sludge
     agricultural utilization practices - an  environmental  assess-
     ment.   EPA Contract 68-01-3265, SCS Engineers,  Long Beach,
     California.   (In  press.)

64.   Lai, C.L., Y.T. Chuang, and S.L.  Sang.  A research  report on
     the treatment of  agriculture and  industrial waste.   Taiwan
     Sugar, 21 :37-41 ,  1974.

65.   Lesoffre et  Cie.   Substituting ammonium  for potassium  in by--'
     products from -the sugar and fermentation Industries.  French
     Patent 1,497^.480, filed March 29,  1966,  awarded October 13,
     1967.

66.   Lopez, J.I., Puerto Rico Distillers.  Personal  communication,
     September 29, 1978.
                               XII-6

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67.   Lopez, J.I.,  Puerto Rico Distillers.   Personal  communication,
     October 4,  1978.

68.   Lyman, C.M.,  K.A.  Kuikan,  and F.  Hale.   Essential  amino-acid
     content of  farm feeds.   J.  Agr.  Food  Chem. ,  4:1008-1113,
     1956.

59.   Maphis, S.W., Briscoe Maphis.  Personal  communication  to  K.
     V.  LaConde, October 10,  1978.

70.   Mattson, M.E.  Membrane  desalting gets  big  push.   Water
     Wastes Eng.,  12:35-42,  April  1975.

71.-  Nelthropp,  C.,  Virgin Islands -Rum Industries,  Limited.   Per-
     sonal communication to  K.V.  LaConde  and  C.J.  Schmidt,  July
     2.7, 1978.

72.   Olszewski,  C.  Separation  of potassium  Compounds  in'view  of
     their reuse.   French Patent  1,377,487,  filed  September  24,
     1963, awarded November  6,  1964.

73.   Oral  testimony  presented at  adjudicatory hearings  for  Virgin
     Islands Rum Industries,  Inc., Puerto  Rico Distillers,  and
     Old San Juan, July 1977.

74.   Page, A.L., Department  of  Soil and  Environmental  Science,
     University  of California,  Riverside.   Personal  communication
     to  K.V. LaConde,  September 26, 1978.

75.   Perez Escolar,  R.   Reclamation of a  saline-sodic  soil  fay  use
     of  molasses and distillery Slops.  J.  Agric.  Univ.  P.R.,
     50:209-217, 1966.

7'6.   Perez Escolar,  R.   Separation and characterization  of  the
     active soil aggregating  agent present  in distillery  slops.
     J.  Agric.  Univ. P.R., 51:304-308, 1967.

77.   Perez Escolar,  R.   Stability of soil  aggregates treated with
     distillery  slops  or blackstrap molasses.  J.  Agric.  Univ.
     P.R., 50:174-185,  1966.

78.   Perez Escolar,  R.   Use  of  molasses  and  distillery  slops with
     sulfur for  the  reclamation of a saline-sodic  soil  from  Puerto
     Rico.  J.  Agric.  Univ.  P.R., 51:55-65,  1967.
                   *•
79.   Phung, T.,  Lv Barker, 0. Ross, and  D.  Bauer.   Land  cultiva-
     tion of industrial wastes  and municipal  solid  Wastes;  state-
     of-the-art  study.   EPA/600/2-78-140a ,  SCS'Engineers ,  Long
     Beach, California, August  1978.   2  vols.
                              X I I - 7

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80.   Puerto Rico Distillers,  Inc.   Proposals  for the  treatment of
     rum distillery wastes.   Submitted with  Schenley  Industries,
     Inc., and Virgin Islands Rum  Industries,  Ltd.

81.   Radhakrishnan, I.,  S.B.  Oe,  and B.  Nath.   Evaluation  of the
     loading parameters  for  anaerobic digestion  of  cane molasses
     distillery wastes.   J.  Water  Pollut.  Control  Fed., 41:R431-
     R440, 1969.

82.   Ramirez Rivera, R.,  and  H.M.  Brau,  Puerto Rico Distillers.
     Personal  communication  to K.V.  LaConde  and  C.J.  Schmidt,
     July 21,  1978.

83.   Reich, G.T.  Production  of carbon and potash  from molasses
     distillery.  Trans.  Am.  Inst.  Chem.  Eng. , 61:233-252,  1945.

84.   Rivera, L.H.   Soil  survey, Virgin Islands of  the United
     States.  U.S. Government Printing Office, Washington,  D.C.,
     1970.  81p.

85.   Roberts,  N'.,  and J.B. Hardwick.  High rate  trickling  filter
     pilot plant studies  in  distillery Waste.   Proc.  Ind.  Waste
     Conf., 6:80-87, 1951.

86.   Roberts,  R.C   Soil  survey, Puerto Rico.   Soil  Survey  Report
     1936 Series,  No. 8,  U.S. Government  Printing  Office,  Washing-
     ton, D.C., 1942.  503p.

87.   Rodriguez, J., Bacardi  Corporation.   Supplemental  testimony
     to the United States  Environmental  Protection  Agency  in the
     matter of National  Pollutant  Discharge  Elimination System
     Permits for Puerto  Rico  Distillers,  Inc., Schenley Industries,
     Inc., and Virgin Islands Rum  Industries,  Ltd.

88.   Rossoff,- G.,  Standard Brands.   Personal  communication  to  K.
     V. LaConde, August  17,  1978.

89.   Rubin, A.J.  Removal  of  trace  metals  by  foam  separation pro-
     cesses.  J. Am. Water Works  Assoc.,  60:832-846,  1968.

90.   Sanford,  D.,  Eco-Research Limited.   Personal  communication,
     August 1978.

91.   Sawyer, C.N., and E.J.  Anderson.  Aerobic treatment of rum
     wastes.  Water-Sewage Works,  96:112-114,  1949.
                 ^
92.   Sen, B.P., and T . R.  Bhaskaran.   Anaerobic digestion of liquid
     molasses  distillery  wastes.   J. Water- Pollut.  Control  Fed.,
     34:1015-1025, 1962.
                              XII-8

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93.
94.
96.
97.
Shea,  T.G.,  E.  Ramos,  J.  Rodriguez- and G.H.  Oorion.   Rum
distillery slops treatment by anaerobic contact process.
Bacardi  Corporation, San  Diego,  Puerto Rico,  July 1974.   109p
(Available from National  Technical  Information  Service (NTIS)
as P8-238 291 ) .
Ski rstymonski i ,  A.I
tial  elimination of
tion.  Perm.  Spirt.
          ,  M.S.  Sushi,  and T.N.  Pukhovaya.   Par-
          salt from vinasse during its  concentra-
          Prom.,  37(7):26-27,  1971.   (Russian).
95.  Skogman, H.
     tion wastes
     Bi ochemi cal
             Effluent treatment of molasses oased fermenta-
              Presented at the Effluent Treatment in the
            Industries Conference, October 1977.
Soldevila, M., and M. Rojas Daporta.
levels of sugar cane molasses on egg
Univ. P.R.,  60:531-634, 1976.
                                        fferent
           Effect  of di
          production.   J.  Agric
Soldevila, M., and R. Irizary.   Influence of dry rum distil
lery slops in diets for white leghorn hens on laying rate,
feed conversion, and egg size and quality.  J.  Agric.  Univ.
P.R., 61:465-469, 1977.
98.
99.
100.
Sopper, W.E., and
Treated Muni ci pal
E.PA/560/2-74-003,
Washington, O.C.,
        L.T.  Kardos,  eds.
        Wastewater through
        U.S.  Environmental
        1974.   463p.
          Conference
          Forest  and
          Protecti on
on Recycli ng
Cropland
Agency ,
Sori gona,
bury,  New
A..B .   The Amamet
York, 1978.   8p.
Process.   Chemapec,  Inc.,  Wood-
Sowa, W.  Trip report for Association of Canadian Distillers'
Third Liquid Feed Symposium, American Feed Manufacturers
Association.  Report ORF-73-2, Ontario Research Foundation,
Ottawa, Canada, 1973.
101. Stern, A.M., L.L. Gasner, and F.A. Sanders.   An economical
     low-energy bio-oxidation process for the treatment of high
     BOO wastewaters,  Proc. Ind. Waste Conf., 29:881-888, 1974.

102. Stros , F., R. Petkvova, V., and V. Varvazovsky.  Desalted
     molasses stillage as cattle feed.   Kvasny Prum.,  13:155-157,
     1967.  (Czech).                                            '-,

103. Struzeski, E.,,-EPA.  Personal communication  to K.V. LaConde,
     October 12, 1^78.

104. Struzeski, E., EPA.  Personal communication  to H. Thron ,
     October 17, 1978.
                               X11 - 9

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105.   Tolaney,  M.   Treatment  of high  strength  citrus  wastewater
      with high purity oxygen activated  sludge process.   Proc.
      Ind. Waste Conf.,  30:171-183,  1975.

106.   Tomczynska,  J.   Activated sludge  as  a  second  stage  of  bio-
      logical  treatment  of wastewaters  from  molasses  slops-process
      feed yeast production.   Pr.  Inst.  Lab.  Badaw.  Przem. Spouzyw.
      24:75-93, 1974.

107.   Tosteson, T.R.,  B.R. Zaidi,  D.  Hale,  and K.  Verner.  The
      effect of the mosto on  the growth  of  marine  microorganisms.
      Report No. RPP:l-73, Agricultural  Experiment  Station,  Uni-
      versity  of Puerto  Rico, Mayaguez,  October 1973.   35p.

108.   U.S. Crop Reporting Board.  Commercial  fertilizers:  consump-
      tion in  the  United States.  Sp. :Cr.  7,  U.S.  Department of
      Agriculture, Washington,  O.C.,  Statistical  Reporting Ser-
      vice, May 1971 .

109.   U.S. Crop Reporting Board.  Commercial  fertilizers:  consump-
      tion of  commercial fertilizers,  primary  plant  nutrients  and
      micronutrients .   Statistical  Bulletin  472,  U.S.  Department
      of Agriculture,  Washington,  D.C.,  November  1976.   64p.

110.   U.S. Environmental Protection  Agency.   Environmental Protec-,
      tion Agency  ocean  dumping regulations  and criteria  (40 CFR
      220-229... .43 FR 1071,  January  6,  1978).  Environ.  Reporter:
      Fed. Regul., 131:8101-8133.

111.   U.S. Environmental Protection  Agency.   Effluent  Guidel.ine-s
      Division.  Development  document  for  interim  final  and  pro-
      posed effluent  limitations guidelines  and new  source perfor-
      mance standards  for the fruits,  vegetables  and  specialties
      segments  of  the  canned  and preserved  fruits  and  vegetables
      point source category.   EPA  440/1-75/046, Group  I,  Phase
      II.  October 1975.  520p.

112.   U.S. Federal Water Pollution  Control  Administration.
      Advanced  waste  treatment  research  program,  July  1964-July.
      1967; 'summary report.   Cincinnati, Ohio, 1968.   103p.
      (Available from  National  Technical  Information  Service (NTIS)
      as PB-216 647).

113.   Virgin Islands  Sewage  Treatment  Plant.   Personal  communica-"
      tion to  K.V. kaConde,  October  13,  1978.

114.   Vitkovskaya, V.A., Y.M. Kravets,  E.S.  Adamenko,  M.D. Lagu-
      tenko, T.I.  Shamatko,  L.R. Zharova,  and  Y.A.  Karanoy.   Bio-
      chemical  purification  of  wastewaters  of  .alcohol  plants pro-
      cessing  molasses.   Biokhim.  Ochistka  Stohnykh.  Vod.  Mater.
      Resp. Konf. , 1974:25-26.

                             XII-10

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115.   Wallach,  A.,  and A.  Wo 1 man.   Treatment  of  distillery wastes
      Sewage Works  J., 14:382-401,  1942.

116.   Wechsler,  I.,  Sala  Magnetics,  Inc.   Personal  communication
      to M.  Montgomery,  September  26,  1978.

117.   Whitaker,  M.C.   U.S.P.  1,504,730.

118.   Zerban,  F.W.,  and  L.  Sattler.   Unfermentable  reducing  sub-
      stances  in molasses.   Identification  of d_-Allulose.  Ind.
      Eng.  Chem.,  34:1180-1188.
                             X11 -11

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         APPENDIX  A





Aquaculture Correspondence
              A-l

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UNIVERSITY  Ol1' CAI.II-'OHNIA
HCKKKLKY • IMViS • IKVtNK • I .OS AWiKI.JvS • KIVERSIOI-: • .fAN WK<:fl • .SA.V KIIANCI.SCO  l|'KBF-JPv«K]oll   SANTA IIAftllAllA • .SANTA C.WV.
                                                        '
AQUACULTUflli PROGRAM                                 '• <>• »ox -1'
BODEGA MARINE LABORATORY                             DOIJECA DAY. CALIFORNIA  S)4923
                                                  (7071 875-3662
                                                 September  22,  1973
     Michael A. Caponigro
     SCS Engineers
     4014 Long Beach  Boulevard
     Long Beach,  CA   90807

     Dear Mr. Caponigro:

          I've examined the material you sent concerning  the waste characteris-
     tics of molasses slops.  My initial judgment  is  that this material is not
     readily adaptable to use for crustacean aquaculture  in terms of a feed
     ingredient.  It  may have some usefulness in a more complex aquaculture
     scheme involving its use as a nutrient source for algae or some sort of
     detrital feeder  which in turn could be utilized  by higher organisms.
     Along these  lines,  I have forwarded the material to  Dr. Michael Hartman,
     Professor, Savannah State College, for his input.  Dr.  Hartman who has
     had extensive  experience with a wide variety  of  aquaculture systems, both
     here and at  the  University of Delaware, is probably  the best suited to
     evaluate any possible utilization of this product in relationship to
     aquaculture.

          If I can  be of further assistance on matters relating to crustacean
     aquaculture  in the future, please don't hesitate to  write.

                                         Sincerely,
                                          /	N
                                           «>•
                                         Douglass. ConJclin
                                         Associate Director
     DEC: ta
     cc:  M.C. Hartman
                                           A-2

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                      SAVANNAH STATE  COLLEGE
                           STATE COCHOt aflAMGH
                           SAVANNAH, QEOROU


  DIVISION or                  October 9,  1978
NATURAL 3CIINCXS
Mr. Michael A.. Caponigro
SCS Engineers
4014 Long Beach Blvd.
Long Beach, California   90807'

Dear Mr. Caponigro:

     Dr. Conklin, a past associate of mine, forwarded your material to me.
After reading over it carefully, I'm afarid that there would not be too"
much use for the waste molasses slops in an aquaculture foodchains.  My
evaluation is based on several factors:  The 800 and COO are very high,
(table 4, p.44) indicating that the material is very high in organic
carbon compounds.  This is further substaniated by the high carbohydrate
content, menti oned. in a Tatar part'of the report. These compunds being
very water  souble enhance the growth of Hetertrophic bacteria which,
besides creating a situation of lower dissolved oxygen, they can destroy  the
bacterial flora of a biological filter system should such a system be in  use.

     Table 4 (p. 45) gives a Mg/L figure for copper as ranging from 2.0-124
with 32.3 as the mean.  A concentration this high would be toxic to most
shellfish, and acutely toxic to the larval  stages.  To introduce this
material into an aquaculture foodchain, should  it be taken up, would further
concentrate the heavy metals.

                                       Sincerely,
                                       Michael Hartnan, Ph.D.
                                       Assistance Professor
                                       Department of Biology
 MH/ls
                                    A-3

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  APPENDIX 3






Anamet Plants
      8-1

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                              Appendix  B
REFERENCE LIST


Anamet plants under operation:


Swedish Sugar Company
Ortofta Factory	

24100  ESLOV - SWEDEN

Capacity

Reduction

Construction year

Attention:  Plant Manager Per Schmidt

Swedish Sugar Company
Hasslarps Factory

Capacity

Reduction

Construction year


Swedish Sugar Company
Karpalund Factory

Capacity

Reduction

Construction year


Swedish Sugar Company
Jordberga Factory

Capacity

Reduction

Construction year
20 ton BOD5/day

98% BOD5

1972
10 ton BOD5/day

95% BOD5

1972
4.5 ton BODj/day

99% BOD5

1975
10 ton BOD5/day

98% BOD5

1975
                                   B-2

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REFERENCE LI3T  (CONTINUED)



AB Skanebrannerier  (Potato Distillery)
Gards Factory	

Capacity                              2 ton BOD5/day

Reduction                             99%

Construction year                     1977
                                    3-3

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ADDRESS LIST
Swedish Sugar Company
Ortofta sockerbruk
241 00 ESLOV - Sweden
Attention:  Plant manager Per Schmidt
Swedish Sugar Company
Hasslarps sockerbruk
260 39 Hasslarp Sweden
Attention:  Plant manager Sven Idoff
Swedish Sugar Company
Karpalunds sockerbruk
29100 Kristianstad Sweden
Attention:  Plant manager Lennart Rantzow
Swedish Sugar Company
Jordberga sockerbruk
230 20 Klagstorp Sweden
Attention:  Plant manager Bengt Lundb.org
AB Skanebrannerier
Box 89
291 01 Kristianstad Sweden
Attention:  Managing director Karl - Axel Kornell
                                    8-4

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ANAMET PLANTS UNDER CONSTRUCTION IN U.S.
American Crystal Sugar Company
Moorhead, Minnesota

Capacity

Reduction

Contact:
19 ton 30D5/day

95% BOD5

Mr-. Ira Fordycs
                                    3-5

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ANAMET PILOT' PLANT' TEST SITES

Universal Foods Corporation
Red Star Yeast Operations
Belle Chasse, LA
Application:  Bakers yeast waste
Contact:  Mr. James L. Martin
Puerto Rico Distillers Inc.
Arecibo, Puerto Rico
Application:  Rum distillery waste
Contact:  Mr. Raul Ramirez
Talpe - Star Foods
Kotemark, Belgium
Application:  Vegetable canning waste
Contact:  Mr. Joseph Talpe
Tirlemont Distillery
Ruisbruek, Belgium
Application:  Molasses distillery waste
Fruit Brandy Distillery
Sewen, Switzerland.
                                    B-6

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