<>EPA
           United States
           Environmental Protection
           Agenc:
           R.S. Kerr Environmental
           Research Laboratory
           Ada, Oklahoma 74820
August 1986
BASIC DESIGN
RATIONALE
FOR ARTIFICIAL
WETLANDS

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             BASIC DESIGN RATIONALE
                       FOR
               ARTIFICIAL WETLANDS
                       by
       John Zirschky and D.  Donald Deemer
               ERM-Southeast,  Inc.
            Marietta, Georgia   30066
             Contract No.  68-01-7108
             Work Assignment Manager

                  Lowell Leach
  R. S. Kerr Environmental Research Laboratory
              Ada, Oklahoma  74820

ROBERT S. KERR ENVIRONMENTAL RESEARCH LABORATORY
      U. S. ENVIRONMENTAL PROTECTION AGENCY
              ADA, OKLAHOMA  74820
          R. E. THOMAS, PROJECT OFFICER
      OFFICE OF MUNICIPAL POLLUTION CONTROL
      U. S. ENVIRONMENTAL PROTECTION AGENCY
             WASHINGTON, D. C. 20460

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                            TABLE OF CONTENTS
                                                                 Page

LIST OF FIGURES                                                   iii
LIST OF TABLES                                                    iii

Section


   1          INTRODUCTION                                          1
              1.1 Background                                        1
              1.2 Objectives                                        2
              1.3 Scope of Work                                     2
              1.4 Definition of Artificial Wetlands                 3
              1.5 Limitations of the Technology                     4
              1.6 Report Format                                     5

   2          LITERATURE REVIEW                                     7
              2.1 Sources of Information                            7
              2.2 Wetlands Hydrology                                8
                  2.2.1  Hydrologic Budget                          8
                  2.2.2  Detention Time                             9
              2.3 Fate of Wastewater Constituents                  11
                  2.3.1  BOD Removal                               11
                  2.3.2  Suspended Solids                          17
                  2.3.3  Nitrogen                                  18
                  2.3.4  Phosphorus                                20
              2.4 Case Studies                                     21
                  2.4.1  General                                   21
                  2.4.2  Listowel, Ontario                         22
                  2.4.3  Santee, CA                                24
                  2.4.4  Arcata, CA                                25
              2.5 Summary                                          27

   3          DESIGN PROCEDURES                                    29
              3.1 Alternatives Available                           29
              3.2 Options for Preapplication Treatment             31
              3.3 Practical Design Guidelines                      36
              3.4 Limiting Constituent Method                      40
              3.5 Detention Time                                   44
              3.6 Plug-Flow First-Order Design Procedures          47
              3.7 Non-Discharge Design                             51
              3.8 Summary of Design Methods                        53

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Section
                        Table of Contents (cont'd)
              RELATED PROCESSES                                     54
              4.1 Introduction                                      54
              4.2 The Root Zone Method  (RZM)                        54
                  4.2.1  Process                                    54
                  4.2.2  Design Procedures                          55
                  4.2.3  Operating Guidance                         58
              4.3 Rock/Reed Filter  (RRF)                            59
                  4.3.1  Process Description                        59
                  4.3.2  Design Information                         60

              RESEARCH CONSIDERATIONS                               61
              5.1 General                                           61
              5.2 Economic Evaluation                               61
              5.3 Field  Research Program                            62

              BIBLIOGRAPHY                                          63
                                     11

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                             LIST OF FIGURES
Figure

 2-1

 3-1


 3-2
Effect of BOD Loading on BOD Removal

Effluent BOD Concentration vs. Influent
BOD Loading Rate

Detention Time Required to Achieve Secondary
Quality Effluent in an Artificial Wetland
Page

  16


  43


  45
Table

 2-1


 3-1


 3-2

 3-3


 3-4
                              LIST OF TABLES
Average Influent and Effluent Values for
    Arcata, California

Characteristics of a Food Processing Wastewater
    used for Design Examples

Design Guide and Checklist for Aquatic Systems

Pretreatment Design Parameters for Use with
    Wetland Treatment Systems

Loading Rates for Wetland Systems
Page


  26


  30

  32


  37

  41
                                    111

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                          SECTION 1

                         INTRODUCTION
1.1  Background

The  U.   S.   EPA  Robert   S.   Kerr   Environmental  Research
Laboratory  (RSKERL)  and the  Beijing  (Peoples Republic of
China)   Municipal   Research   Institute   of   Environmental
Protection (BMRIEP), as part of a cooperative wastewater land
treatment  program,  are currently evaluating  the  feasibility
of  using  artificial  wetlands  for  the  treatment  of  high
strength wastewaters.   Of  particular  interest is  the  use of
wetlands   for   treating   brewery   and   food   processing
wastewaters.    The effluent  from  the  wetlands could  then  be
used  for  crop  irrigation.    Re-use  of  the  effluent  as
manufacturing process water or as a drinking  water  source are
also being considered.

Although  some  research  on  the  efficiency  of   artificial
wetlands treatment has  been conducted in the  United  States,
specific   design   guidance    has    not   been    published.
Furthermore,  operational data from artificial wetland  systems
are sparse.   By  attempting  to compile a basic guideline for
the design of  artificial wetlands  for the treatment  of  high
strength  organic  wastewaters,  areas  in  need   of   further
investigative study can be  identified.  The  design rationale
developed  can  be used  to  determine  if further  co-operative
research of this treatment mode is warranted,  based upon any
deficiencies  identified in  the design  procedures.

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1.2  Objectives

The objectives  of  this  work assignment were first to  review
the  available   literature   on   the  design  of   artificial
wetlands, and  second,  to prepare a document outlining  basic
design  rationale  for  utilizing  artificial wetlands to  treat
high  strength  wastewaters  from  brewery  or  food  processing
plants..   The  criteria  developed would  serve  two purposes.
First, they will assist in determining if further  cooperative
research on  wetlands  systems should  be  conducted.   Second,
these criteria will also assist  EPA's  Office  of Water  Program
Operations in implementing innovative  and alternative  aquatic
treatment systems through the Construction Grants  program.
1.3  Scope of Work

In order to develop the design rationale,  a  computer abstract
search was  conducted  to identify literature  related  to the
design  and   operation  of  artificial  wetlands  treatment
systems.    In  addition,  contact  was  made  with  several
individuals known  to have  conducted  research  related to
artificial wetlands.  Design alternatives  were  then generated
based  upon the  information  collected and a draft  report
summarizing these  alternatives  was  submitted  to  EPA.    This
report  incorporates  the  comments   received   on  the   draft
report.   To  facilitate further research  in this  area, a
bibliography of wetlands references  was also prepared and is
included in this report.

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1.4  Definition of Artificial Wetlands

The  computer   abstract   search   was   designed  to   include
information on  wastewater treatment in  natural  wetlands as
well as  in artificial wetlands.   The  purpose of  including
natural wetlands in the literature review was  to ensure  that
as much data as possible  relevant  to the design of  artificial
wetlands  was  obtained.   Upon reviewing  the   literature, it
became apparent that a number of different types of systems,
such as  marshes,  swamps,  peat  bogs,  and  water  hyacinth
systems were referred  to  as  wetland treatment  systems.   The
size,  shape,  and water depth of these systems are  highly
variable.

In this report,  the  term artificial wetlands  is  limited to
systems with the following characteristics:

     1)   Areas  in  which rainfall  and the  influent wastewater
          are  the only water sources.   Water losses occur
          only    by    treated   wastewater    outflow   and
          evapotranspiration.

     2)   Systems  which are  based upon  plug  flow  hydraulics
          with  a single outflow.   Systems which  discharge
          primarily  to ground water, such  as  cypress  domes,
          are not  included.

     3)   Systems  where the  water  surface is  at or  above the
          ground surface   long  enough  each year to maintain
          saturated    soil    conditions   and   the   related
          vegetation  (Reed,  pers.  comm.).

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1.5  Limitations of the Technology

Although  limited  data are available  on  the use  of  wetlands
for treating primary  effluent,  artificial  wetlands have  been
used  in  a  number  of  locations  for  polishing  secondary
effluent.  Applied BOD concentrations are,  thus,  typically  on
the order of  30 mg/L  or  less.  Food  processing  wastewaters,
on  the  other hand,  may contain  BOD  concentrations  ranging
from 300 to greater than 30,000 mg/L.   In addition, some  food
processing  wastewaters  may   contain  significantly   higher
concentrations  of  solids,   nitrogen, and  phosphorus  than
typical domestic sewage while  others  are substantially lower
in  nutrients  than domestic  sewage.    Design procedures  for
utilizing  wetlands,  normally a  polishing  process,  as  the
primary treatment  process for  high  strength wastewater  were
not found in the literature.

Individuals active in the research of  artificial  wetlands  as
well as  the  environmental  representatives  of  several  food
processing  industries  and  trade  associations  were   also
contacted.   None  of  these  individuals were  optimistic about
the use  of  artificial  wetlands  alone  for treating high
strength  wastes.      The  concensus   of   opinion  was   that
pretreatment would  be required  in  order  to use artificial
wetlands.

It was the original intent of  this report  to exclude  systems
in which only subsurface  flow occurs  (e.g.  rock/reed filters,
the Root Zone method) .   In  a strict  sense, such  systems  are
not artificial wetlands because the water surface  is never  at
or above the  ground  surface  and  the  removal rates  in these
systems  are substantially  different from those found  in

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 artificial wetlands.   The objective of this  report is  to
 present  potential  design  procedures  for  artificial  wetlands.
 As  requested  by  EPA, however, design  information on  the  root
 zone method  and the  rock/reed filter  are  also  presented
 herein  (Section  4).

 It  was also  initially intended to  present  as many design
 procedures as possible to assist the RSKERL and  the  BMRIEP in
 developing a  joint  research program.   After  discussions  with
 EPA,  however, several  of  these  methods have  been  eliminated.
 The  amount of information or assumptions  required  to  use  such
methods  would seldom, if  ever,  be  available to  a design
engineer.   It is important to  note  that  some of the design
procedures contained herein may also  be  inappropriate, since
many  of  these procedures have never been verified.   One  goal
of  any  subsequent  research  on artificial wetlands should  be
to  attempt to validate  these  methods and determine which
methods yield accurate  results.
1.6 Report Format

This  investigation  has  concluded  that  artificial  wetlands
alone will  not  provide adequate treatment  of  high strength
wastewaters.  Thus,  in order to be used, artificial wetlands
must be included as one  step  in an overall treatment scheme
which would include pretreatment and perhaps post-treatment.
In  Section  2,  a brief  review  of  the  literature  related to
artificial  wetlands  is  presented.     Some  information  on
natural wetlands is also  included  in  this  section since the
treatment processes  in artificial  and  natural  wetlands are
similar; although,  the rate at which  these  processes  occur

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may  differ  significantly  between  artificial  and  natural
wetlands.    In  Section  3,   potential  design  methods  are
presented  along  with a  brief  discussion  of pretreatment
options.  Section 4 discusses  other treatment processes which
are related to wetlands  and  which could be used for treatment
of  food  processing wastes  (after  pretreatment).    Research
recommendations  are  presented in  Section 5.   Finally,  a
bibliography of wetlands and related information is presented
in Section  6.

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                          SECTION 2
                      LITERATURE REVIEW

 2.1   Sources of Information

 A   computer   abstract   search  was  conducted   to   identify
 references related  to  the design and performance of  natural
 and  artificial wetlands  treatment  systems.   Abstract  sources
 searched  were  NTIS, Pollution Abstracts,  and  Agricola.    In
 addition,  research  facilities  and  individuals  known  to  have
 sponsored  or  conducted  research  on artificial wetlands  were
 also  contacted.  Facilities contacted  included  RSKERL,  the  U.
 S.  Army Cold Regions  Research  and  Engineering Laboratory
 (CRREL),  and several  universities.   The results  of this
 literature review are summarized  briefly  in this  section.

 Literature on the design of artificial wetlands  for treating
high-strength  wastewaters  was  not  found.     Research   on
 artificial wetlands has  focused primarily on the  treatment  of
 secondary  municipal  effluents.     Very   limited  data  are
available  on  the treatment  of raw and  primary wastewaters
using  artificial   wetlands.     The   systems  treating  raw
wastewater are  not  conventional  artificial wetlands.   For
example, in the  Root Zone method (Lawson,  1985), wastewater
flows beneath the soil surface through the root  zone  of the
vegetation.    Surface  flow does not occur,  and   vegetation
plays  a minimal  role  in  purification  of the  wastewater.
Lawson  (1985) himself  states  that  the  Root Zone method  is
distinctly different from artificial wetlands.  Discussion  of
the  Root   Zone  method  is thus  presented separately from
conventional  artificial  wetlands.   The  following discussion
is based upon key literature  pertaining  to the treatment  of
wastewater in artificial  wetlands, as  well as  in  natural

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 wetlands.   A general discussion of the  fate  of  contaminants
 is  presented  first.   Brief  case studies  of  several  key
 research  investigations are then discussed.
 2.2  Wetlands Hydrology

     2.2.1   Hydrologic Budget

 The  performance   of  any  artificial  wetlands  system   is
 dependent upon the system hydrology as well as other  factors.
 Precipitation,  infiltration,  evapotranspiration,  hydraulic
 loading  rate, and  water  depth  can  all affect the removal  of
 organics, nutrients, and trace elements not only by  altering
 the  detention time, but  also by either  concentrating  or
 diluting the wastewater.   A  hydrologic  budget should  be
 prepared  to  design an artificial  wetlands treatment system
 properly.  Changes in the detention time or water volume can
 significantly affect the  treatment  performance.

 For  an  artificial  wetlands  as  defined  in  Section  1.4,  the
water balance could be expressed  as follows:

     Qi - QQ + P  -  ET = dV/dt                        [Eq 2-1]

where:

     Q.  = influent  wastewater flow, vol/time,
     Q   = effluent  wastewater flow, vol/time,
      P = precipitation,  vol/time,
     ET = evapotranspiration, vol/time,
     V = volume  of water,  and
      t = time.

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Artificial  wetlands  designed  for wastewater  treatment  are
generally constructed above  an  impermeable surface  (e.g.,  a
liner  or  natural  soil barrier),  and  under such  conditions,
ground water inflow and infiltration would not occur.   Thus,
these  components  of  flow are not  included in Equation  2-1.
Historical   climatic   records   can  be   used  to   estimate
precipitation and evapotranspiration.   Empirical methods such
as  the  Thornthwaite  equation  can  be   used  to   estimate
evapotranspiration  (Thornthwaite and Mather,  1957).   Pan
evaporation  measurements may be  useful if the wetlands  will
contain a significant percentage  of  open  water areas.     If
required,  estimates of water  losses  due to infiltration  can
be obtained  by conducting  infiltration  tests such  as  outlined
in  the EPA  Process Design  Manual  for  Land Treatment of
Municipal  Wastewater  (EPA,  1981).    Then,  if  the system
operates at  a relatively constant water depth  (dV/dt=0),  the
effluent flow rate can be  estimated using  Equation 2-1.
     2.2.2  Detention Time

Treatment performance in artificial  wetlands  is  a  function of
the detention time,  among other  factors.   Ground slope, water
depth,  vegetation, areal extent, and geometric shape control
the flow  velocity and,  thus,  the  detention  time  through a
wetlands treatment system.

In both  natural  and  artificial  wetland  systems,  estimating
the detention time  can  be difficult  for several  reasons.
First,  large  dead  spaces may exist  in the  wetlands  due to
differences    in     topography,    plant     growth,    solids
sedimentation, and  the degree  of  flow channelization  (i.e.
short-circuiting).   Only a  fraction  of the surface area, in
both artificial and  natural wetlands,  may  be available  for

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wastewater  flow.   Wile  et al. (1985)  reported that  in  spite
of    accurate    leveling   of   an    artificial    wetlands,
short-circuiting still occurred.
The ratio of the surface area available for flow to the  total
surface  area  is termed  the  void fraction.   Using  the  void
fraction,  residence time  for wetlands  can be  defined  as
follows  (Hammer and Kadlec, 1983):
                                                    [Eg.  2-2]
where :

     0 =  residence time,
     V =  volume of water in the wetland,
     Q =  volumetric flow rate,
     A =  surface area of the wetland,
     d =  average water depth, and
     0 =  void fraction of surface area  available  for  flow.

The void fraction, 0,  is a difficult parameter to estimate.
Hammer and  Kadlec (1983)  cite values  for  the  void  fraction of
0.1 to 0.3 for  shallow water sedge meadows  to  approximately
1.0 for  deep, open  water  wetlands.   Black et  al.  (1981)
reported that dense  cattail growths  could occupy  between
one-third to  one-half of  the  wetland  volume.    Dead spaces
within the  cattails can also occur.

Since   the   void  fraction  can   be   difficult   to  estimate,
especially  when  data  on the  topography and  water depth are
                              10

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lacking,  a superficial  residence  time,  6s,   is  sometimes
used,  which  is defined by  Equation  2-3  (Hammer and  Kadlec,
1983) .
     9s =                                           [Eq.  2-3]
In  estimating  the  actual  or superficial residence time, the
flow rate, q,  used  should be  an  average of the influent and
effluent  flow rates  since  water losses (or gains)  can be
significant.

Treatment performance is a function  of detention time in the
wetland.   Wile  et al.  (1985)  reported seven days  to be
optimal for  the  treatment of  advanced primary and secondary
wastewater.  Shorter detention times do not provide adequate
time for  pollutant degradation to  occur; whereas,  longer
detention times can  lead  to stagnant, anaerobic conditions.
Two climatic factors  can  significantly  affect the detention
time at a constant hydraulic loading rate.   In warm weather,
evapotranspiration  can  significantly  increase the detention
time.   On  the  other hand, ice  formation in  cold weather can
significantly  decrease  the detention   time.    Maintaining
liquid  depths of less than 10  cm in  warm weather and greater
than 30 cm in  cold weather  was  recommended  to  minimize the
effect  of climate on the detention time.
2.3  Fate of Wastewater Constituents

     2.3.1  BOD Removal

The treatment processes which occur in an artificial wetlands
are similar to those  which occur  in other  forms of  land

                             11

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 treatment.   Removal of settleable organics occurs  primarily
 as   a   result   of   sedimentation   and   aerobic/anaerobic
 decomposition  in the  sediments.   Removal  of  colloidal  and
 soluble   organics   occurs   primarily  by   aerobic   microbial
 oxidation.  Few data are available for the removal  of  BOD  and
 COD  in  wetlands,  and there are some  limitations in the data
 that are  available.   The  primary limitation is that much  of
 the  available data  are from  natural  or  artificial  wetland
 systems used  to  polish municipal advanced  secondary  quality
 effluent,  primarily  for nutrient removal.  BOD loadings  are
 thus considerably less  than would be  experienced in a  wetland
 treating  food processing  wastes.    Some of  these systems
 report  BOD removals of over  80 percent  (Gersberg,  et al.,
 1984a;  Fetter, et  al., 1978),  while other systems  report
 essentially no BOD removal  (Stowell,  et  al.,  1980).

 The low influent BOD concentrations  applied to many wetlands
 systems discussed  in  the  literature may be very close  to
 background levels;  thus,  little or no  reduction  in  BOD
 concentration would be expected.   When  significant water
 losses  due  to evapotranspiration occur,  however,  the mass
 removal of BOD may be significant.

Data on the removal of  BOD from primary municipal   wastewater
 is available primarily  from two systems:   Santee, California,
and Listowel,  Ontario.    No data were found on the  treatment
of raw municipal wastewater  (note: methods  such as the Root
 Zone  treatment   scheme   used   in   Europe   are   discussed
separately).   Black  et al.  (1981)  reported 87 percent  BOD
removal for an influent BOD quality  of 65.5 mg/L (effluent -
9.0 mg/L).   The  estimated  average  detention time  in  the
wetland system was  7.8 days.   Additional  and  more detailed
information on the  performance of this system will soon  be
published  (Herskowitz,  pers.  comm.).
                              12

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 At  Santee, California,  a  mixture of  primary and  secondary
 municipal  effluents was treated using an artificial wetlands.
 The  average  influent  BOD concentration was 49 mg/L while  the
 effluent   BOD   concentration   was  approximately   3.2   mg/L.
 Wastewater was  applied at  the  rate  of  18  cm/day.    When
 primary wastewater  alone was treated (influent BOD  =  148
 mg/L),  the  effluent BOD  averaged  approximately 33  mg/L.
 Primary wastewater was applied at a hydraulic loading  rate of
 6 to 8.3 cm/day.  Better year-round performance  occurred at a
 hydraulic  loading  rate  of  6 cm/day,  which corresponded to  a
 detention  time of 1 day (Gersberg et  al.f  1984a).   Additional
 data  from the  Santee  and  Listowel  research projects  are
 discussed  in Section 2.4.

 BOD removal  in  wetlands  has  been described by a  first-order
 model as follows (Lakshman,  1980) :
     ln(C/Co) = -kt                                 [Eq.  2-4]

where

     C =  BOD concentration at  time  t,
    C  =  initial BOD concentration,
     o
     k =  decay constant,  and
     t =  time.

The first-order  kinetics  of BOD  removal  in wetlands systems
are similar to those found with both overland flow and lagoon
systems, both  of  which  are somewhat analogous  to artificial
wetlands.  The data from Listowel and  Santee presented above
can be used to estimate the decay constant for  Equation  2-4.
At Listowel,  the  decay  constant  would be  equal  to 0.25/day
                              13

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 (year-round  average) .   At Santee the  average  decay rate was
 1.5/day  for  primary  wastewater.   Lakshman  (1980)  reported
 decay  constants equal  to 0.25/day  (September)  and 0.14/day
 (October)  for initial BOD concentrations  ranging  from 30 to
 100 mg/L.

 Sufficient  information,  such  as  temperature  data, are  not
 currently available to  permit an in-depth comparison of these
 decay  rates.   One  would expect  a lower year-round  decay rate
 for  Ontario  (Listowel)  than for  California  (Santee).   An
 investigation  of  the  effects  of  the  wetlands  and climatic
 characteristics  on the decay rate  may be  a  prerequisite to
 developing  rational  design  procedures.    The  forthcoming
 report on the Listowel system may contain such information.

 Gearheart,  et al.  (1983),  conducted  a  pilot-scale test  of
 artificial wetlands for polishing secondary effluent and also
 found  BOD  removal  to  be  described  by a  first-order  kinetic
model:
     -In ^  = (fhko) Q

     S  = effluent concentraton,  mg/L,
     S. = influent concentration, mg/L,
     h =  thickness of slime, m,
     W =  width of section (width of cell) ,  m,
     Q =  volumetric flow rate, m /day,
     Z =  filter depth (length of cell),  m,
    k  =  maximum reaction rate,  /day,
     o
     f =  proportionality factor
                              14

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The  term  (fhk  )  was  found  to  be approximately equal to  4.95
m/d  when oxygen  was  not limiting.   Under oxygen limiting
conditions,  BOD  removal occurred at  a significantly  slower
rate.   The decay  rate was not reported, but it appeared  from
the  data presented  to  be approximately one-fourth of the
non-oxygen limited decay rate.

Equations 2-4 and  2-5 are  very similar.    If  the  slime
thickness in  Equation  2-5 was  known, one could  rearrange
Equation  2-5 into  the form  of  Equation 2-4  and then compare
the  rate  constants in each equation.   The  slime  thickness,
however,  is  not  defined by Gearheart  et al.  (1983);  thus,
such a comparison is  not possible.

The decay rates used  in  either  Equation 2-4 or  2-5 may not be
indicative of  the  performance  which might be  expected  for a
wetland system  treating  higher  than advanced primary strength
wastewaters.   Higher  BOD influent concentrations could create
extensive anaerobic  conditions,  and  BOD oxidation  would be
expected  to  be less  under  anaerobic  conditions  than  under
aerobic conditions.  If  anaerobic conditions  do occur,  odors
could be  a significant problem.  To reduce  the potential for
odors, Stowell, et al, (1980)  recommend BOD  loading rates of
60 to 70 kg/ha/d  or less;  although, one  example of a wetlands
system treating 100 kg/ha/day without odors was cited.

The  relationship  between  BOD  loading  and removal  rates has
been evaluated  by  Stowell et al.  (1980)  and  is presented in
Figure  2-1.   It  is important  to recognize  that  only five
systems were  evaluated in  compiling Figure 2-1 and that these
systems   treated   secondary  municipal   or   better  quality
                              15

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   40
   30
Q
O
CD
h-  20
UJ
D
_l
U_
u_
UJ
   10
          DATA FROM 17 DIFFERENT  STUDIES
   o


o   o
          o
         o
       o
               50       100        150


                 INFLUENT BOD (mg/L)
                                   200
    Figure  2-1.
       Effect of BOD Loading on BOD Removal

       (Marsh and Peatland Systems; Stowell,
       et al.,  1980).
                        16

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effluent.  The data  suggest  that  the degree of removal is  a
fairly linear function of  the  loading applied.  Data  beyond
an applied loading rate of 100  kg/ha/day  are not presented  in
Figure 2-1.  The implications of this data  base are  that if  a
desired effluent load is selected, the influent loading rate
could  be  determined.   The  relationship  may  continue  to  be
linear well  beyond  the range  of  the data,  and  when  higher
effluent  values  can be  tolerated,  the influent  loadings
could,  perhaps,   extend   well   above  100   kg/ha/day.    For
example,  Gersberg et  al.  (1984b)  used BOD loading  rates  of
116 kg/ha/day without  any reported  problems.   The effluent
flow rate at this loading rate  is not presented, however,  to
permit calculation of a mass  removal  rate.

     2.3.2  Suspended Solids

The suspended solids present in a wastewater will be removed
by primary sedimentation in  a wetland.   If the  influent
solids loading  is high,   significant  accumulation  of  solids
could occur near the influent discharge points. An  ugly gray
slime  accumulation  was reported  to  have  occurred  near the
influent  to a wetlands system in Ontario, Canada, receiving  a
high  solids  wastewater   (Huggins,  personal  communication).
Degradation  of  the  solids  can also create  a significant
oxygen demand.   As a result,  anoxic  conditions  could develop,
thereby generating  odors  and killing vegetation.   Black  et
al.  (1981)  noted such conditions  occurring as a  result  of
solids accumulation  at the Listowel  system.

If the water  in  the  wetlands  is not  shielded from sunlight by
the vegetation,  algae could  also be  a problem  in warm months.
Algal solids will not  settle readily and could lead to high
suspended  solids concentrations in  the effluent.   If the
effluent  is  to  be used for  crop  irrigation,  however,  algae
may not be of concern.   Furthermore, during daylight  hours,
                              17

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the algae can generate significant quantities  of oxygen.   At
night, however, respiration can consume  the  oxygen, leading
to anaerobic conditions.   Gearheart,  et  al.   (1983) reported
that 20 to 40 percent open water was optimal  for wetlands as
the  resulting  phytoplankton   can  help  control  pathogens
without creating significant algae  problems.
     2.3.3  Nitrogen

Microbial  action   (nitrification-denitrification)  and  plant
uptake  are  the  primary  nitrogen  removal  mechanisms  in
wetlands.  Depending  upon the detention time and pH, ammonia
volatilization may  also be significant.   Stowell et  al.
(1980)  reported nitrification rates  ranging from  0  to 45
kg/ha/day  and denitrification rates  ranging from  0  to 40
kg/ha/day.   Vegetation,  however,  was reported to remove only
approximately 6 kg N/ha/day.  Wile et  al.  (1985)  reported
that  vegetation  removed  only  8   percent   of  the  applied
nitrogen at the Listowel  system.

Nitrification and denitrification  occur primarily at  the
interface between  the soil particles or  vegetation  and  the
water. Thus, the depth of water,  the area inundated, and the
presence of vegetation can affect the rates of nitrification
and denitrification.   Weber and Tchobanoglous  (1982) reported
nitrification to  be  a function  of  the  application  rate in
water hyacinth  systems.    As  the  application rate increases,
the  transport  of  ammonia to  the  nitrifying  organisms  which
grow  on the  soil and  plant  surfaces  also  increases.   A
similar  phenomenon may   occur  in  artificial wetlands.   For
example, shallow water depths  and  even flow distribution in
wetlands encourage  contact  between  the  wastewater  and  the
microorganisms  which  grow on  the  soil  particles  and  plant
surfaces  (Kadlec  and Tilton,  1979) .    Shallow  water depths
                             18

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also promote the  transport  of  oxygen  from the water surface
to the nitrifying organisms.

Zoltek,  et  al.   (1979)  conducted  a laboratory study  of the
effects  of  water  depth,   pH   control,   and   vegetation  on
nitrification rates  in natural  marsh  soils.  At  ammonia
nitrogen concentrations  of 40  to 50 mg/L,  ammonia removal
rates of 4.9 (15 cm water depth), 5.7 (30 cm), and 7.7  (0 to
30 cm  variable  depth)  kg  N/ha/day were  found with plants.
Lower  rates  were  generally reported in  the  systems without
plants.   Controlling  the pH  to  the   range of  7 to 8.5
increased ammonia removal to 14.3 kg N/ha/day  in the systems
with plants.  As the  strength of  the  influent wastewater
increases,  the  nitrification  rates  might  be expected  to
decrease  since   oxygen   may   become   too   limiting   for
nitrification to occur.  More complex organics may be broken
down  into  ammonia  and, without  significant  nitrification,
ammonia may accumulate.

Some ammonia  could  be  removed  via volatilization  depending
upon the detention time  and the pH.  Ammonia  volatilization
increases with increasing pH and detention time.    The  pH of
one artificial wetland  was found to be  neutral  to slightly
acidic   (Gearheart  et  al.,  1983),  and  since  artificial
wetlands typically have  detention times less  than  10   days,
ammonia  volatilization  would   not  be   expected   to  be  a
significant  removal  mechanism.   Procedures for  estimating the
amount of ammonia volatilized are  presented by  Reed  (1984).

The presence  of  high  ammonia  concentrations  is  of concern
because  ammonia  is  toxic to aquatic  life.    Fish  are   often
used  for mosquito  control  in  wetlands, and high  ammonia
levels could cause  fish kills  in the  areas  where  there  is
sufficient dissolved  oxygen to  support aquatic  life.
                              19

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Gersberg  et al.  (1984a,b)  found  that by  increasing  the
influent BOD concentration (by selective blending of primary
and secondary wastewater), nitrogen  removal in wetlands could
also be  increased.    The addition of  plant biomass  also
stimulated   nitrogen   removal.      Total   nitrogen   removal
efficiencies of 90% were obtained  in  wetlands where  both
primary wastewater and  plant  biomass were used  as  a carbon
source.  Although higher  strength wastewaters should provide
sufficient  carbon for  denitrification (and thus  nitrogen
removal)  to  occur, the  resulting  anaerobic  conditions could
limit  nitrification.     In  fact,   limiting   the  extent  of
anaerobic  conditions   over much  of  the  wetlands  may  be
difficult in a  wetlands  treating higher  than primary strength
wastewater.  Gersberg  et al. (1984a)  found that  nitrogen
removal decreased to  only 25 percent when  primary  strength
wastewater was  treated.

Only the Listowel data  are sufficient for estimating the rate
of nitrogen  removal.   At  a detention  time  of approximately
7.8 days, the  total nitrogen  concentration  was  reduced  from
21.1 to 8.45 mg/L.   If  first-order  kinetics  are applicable,
then the first-order decay rate for  nitrogen removal would be
approximately 0.12/day  (year-round average).
     2.3.4  Phosphorus

Removal  of phosphorus in wetlands occurs  primarily  as  a
result  of  soil  adsorption  and  to  a  lesser  extent  by
precipitation  and  plant   uptake.    Phosphorus  removal  is
enhanced by:  1)  organic soils in  poor  nutrient regimes, 2)
vegetation which  is  either limited  in  phosphorus  supply or
capable of  luxury consumption,  and  3)   the  presence  of  iron
and aluminum  (Heliotis,  1982).   As  the water  depth  in the
wetland decreases,  the potential for contact  with  the  soil
                              20

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surface and, thus, adsorption increases.   With  time, however,
the available adsorption  sites will  become  saturated and
phosphorus removal will decrease.   Hammer and Kadlec  (1983)
presented a procedure  for  estimating  the rate of phosphorus
saturation in natural wetlands.   This  procedure  may also be
applicable to artificial wetlands  since the chemical/physical
processes should  be  similar  in both  natural  and artificial
wetlands.

Phosphorus  removal  is affected  by temperature with most
phosphorus removal occurring  in summer,  and  some phosphorus
release  occurring  in winter (Fetter  et al.,  1978),  due
perhaps  to  release  from  decaying  vegetation.   Black  et al.
(1981)  also  reported  that phosphorus removal decreased  during
colder weather,  as did Gearheart et al.  (1983).  Wile  et al.
(1985)  reported  that  vegetation  removed   only  10  percent of
the applied phosphorus.  Reed (pers.  comm.)  recommends  that
to be  conservative, one should assume  that  no phosphorus
removal will occur.
2.4  Case Studies

     2.4.1  General

Several artificial wetland  systems  are in  operation  in the
United States.  Most of these systems, however, are designed
either to treat secondary or higher quality wastewater or to
be non-discharging systems.   Examples of  such systems are:
Incline Village,  Nevada  (Williams  et al.,  1985);  Mountain
View,  California   (Demgen,   1979;   Hyde,  et  al,   1984);
Vermontville,  Michigan (Williams  and  Sutherland, 1979;  Bevis,
1981; and Hyde  et  al.,  1984); Neshaminy Falls, Pennsylvania
(Hyde et  al.,  1984);  Las Galinas  Valley  Sanitary  District,
California  (Demgen,  1984); Cannon  Beach, Oregon  (Demgen,
                              21

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1984); and Arcata, California (Gearheart  et  al.,  1983).

Only   two   systems  treating   primary   strength  municipal
wastewater were  found  in  the literature:   Listowel, Ontario
and Santee,  California.   Both  of  these  systems are briefly
discussed herein.   The Root  Zone  treatment method  used  in
Europe is not discussed in this section  because  such systems
are not  artificial wetlands   (Lawson, 1985) .   The Root  Zone
method is discussed separately in Section 4.
     2.4.2  Listowel, Ontario (Black  et  al.,  1981;
            Wile et al.,  1985)

In  the  late  1970's,  the  town  of  Listowel's  wastewater
treatment system was  becoming hydraulically overloaded.   The
treatment system  consisted  of  an  aerated  lagoon  with  a
detention time  of  3.5  days  followed  by  two  facultative
lagoons  in  series,  each  with  a  detention  time  of  35 days.
Upgrading the system to treat the increasing  load and  meeting
water quality standards by conventional means appeared to  be
uneconomical for  this small community.  A pilot  study  of
using artificial wetlands  for  polishing the lagoon effluent
was  thus  undertaken  in  hopes  of  finding a  more economical
means of upgrading the treatment system.

The pilot system was constructed to  treat the effluent  from
both the  aerobic and facultative lagoons.    The performance
of  the  systems  treating  the  aerated  lagoon effluent  are
applicable to this report.  Two  basic wetland configurations
were utilized, one a series of  five long narrow basins with a
length to width ratio of 75:1 and the other,  one  basin with a
length to width  ratio of 4.5:1.   The  series  of  long  narrow
basins consistently performed better  than the  one long basin,
and the researchers  recommended that  future  systems  utilize a
                              22

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similar  geometry.     Thus,   this  basin  configuration   is
discussed herein.

In  the  literature  related   to  this   system,   the  authors
describe the  effluent  from the  aerobic  lagoon as  "raw,
aerated  sewage"  (Wile et  al.   1985),  thus  perhaps  implying
that the wastewater only  undergoes minimal treatment  prior  to
the  wetlands.    Data  from   the   first year  of  operation
indicates that the effluent BOD  and TSS from  the  aerated cell
are approximately  65.5 and 119  mg/L, respectively (Black  et
al.,  1981).    The  wastewater   thus  undergoes  significant
treatment prior to the wetlands.

Wile et  al.  (1985)  present performance data  for  this system
for approximately the first 1-1/2 years of operation (August
1980 to  April 1982).  During  this  time period  the average
effluent    (from   the   wetland)    BOD  concentration   was
approximately 10.7 mg/L for an  average  removal efficiency  of
approximately 86 percent.  The  effluent  TSS concentrations
averaged 8.2 mg/L for a 94 percent removal efficiency.  Total
nitrogen  removal   averaged   66  percent   for  an   effluent
concentration of  8.3  mg/L.  The  influent concentrations for
these  parameters  were  not   reported.   From   the  removal
efficiencies  cited,  however,  the  approximate  influent BOD,
TSS, and total nitrogen concentrations  were approximately 76,
130, and 25  mg/L,  respectively.   The  average  hydraulic
loading  rate  was  2.5  cm/day yielding  organic  and  nitrogen
loading  rates  of 18.4 kg  BOD/ha/day and 6,04  kg N/ha/day.
Total  phosphorus removal was cited as 98  percent.  Alum,
however, was  used  to precipitate  the  phosphorus.   A report
summarizing the  four years  of experimental  data  at  this
system  should be available in September,  1986.
                              23

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     2.4.3 Santee,  California  (Gersberg et al.,
           1983,1984a,b)

Several studies  on  the  performance  of  artificial  wetlands
have   been   conducted   at  the   Santee,   California   Water
Reclamation Facility.  Most of  the investigations conducted
at  this facility  focused on nitrogen  removal  in artificial
wetlands.    Secondary effluent was primarily  treated  during
these  investigations; although,  some  research  was conducted
using primary and a blend of primary and secondary effluent.
Two beds,  each 71 m long by  11.6 m  wide  (L:W=6.1:1)  were
used.  Wastewater was applied  at  hydraulic loading rates of 6
to 20 cm/day.

The objective  of mixing  primary  and  secondary effluents was
to provide a source of carbon to enhance nitrogen removal in
wetlands;  thus, the majority of  the investigations focused on
nitrogen removal.  Gersberg et  al. (1984b)  reported  that if
the goal of the wetlands  treatment is to produce an effluent
quality to  less  than 10  mg/L of  total  nitrogen,  then blend
ratios of  1:2  (pr imary: secondary)  can be used  at hydraulic
loading  rates  of  18  to 19  cm/day.   At this blend,  the
influent  BOD quality  tested  was  approximately  63.5 mg/L,
which corresponds to a  mass  loading rate of approximately 116
kg  BOD/ha/day.   At this  loading rate,  approximately 89
percent of the influent BOD was removed yielding an effluent
concentration  of approximately  7 mg/L.   Total  nitrogen was
reduced  from  24  to 5 mg/L at  a  loading rate of 44.4 kg
N/ha/day (79% removal).

An artificial wetlands treating a  1:2 blend with an influent
BOD concentration of 49  mg/L at  a  loading  rate  of 18  cm/day
was  reported  by Gersberg et  al.  (1984a)   to produce an
effluent  quality of 3.2 mg/L (93%  removal).   The organic
                              24

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loading rate was  approximately  88.2  kg BOD/ha/day.   At  the
1:3.8 primary to secondary blend at a  loading  rate  of 18-19
cm/day,  influent  BOD   concentrations  were   reduced  from
approximately 40.9 mg/L to 4.1 mg/L (90%  removal).   The  BOD
loading  rate was  approximately  77  kg BOD/ha/day.   Total
nitrogen  reduction  data were not  reported  for  the  1:3.8
blend.  Straight primary  wastewater with  a mean BOD  of  148
mg/L  was  treated at a hydraulic loading  rate of 6  to  8.3
cm/day (106 kg/ha/day).   The  mean effluent concentration  was
33 mg/L corresponding to  78 percent BOD removal.   Treatment
performance decreased, however,  when  the  hydraulic  loading
rate  was  increased from  6 to  8.3 cm/day.   Total nitrogen
removal decreased  to  only 25 percent.  Neither detention time
data nor hydrologic data  were presented to permit calculation
of the decay rates  or mass removals.
     2.4.4  Arcata,  CA  (Gearheart, et al., 1983)

The city of Arcata,  California,  conducted a  three-year  pilot
project of  wetlands treatment of  secondary  effluent.    The
major  goal was  to  prove  that  the  effluent from  a marsh
receiving  secondary  effluent  could  enhance  the  water  quality
in Humboldt Bay.   A discussion  of  this system  is  included
primarily  to provide supporting information for Equation 2-5.

The marsh project  was  constructed  adjacent  to  the  city
oxidation  ponds.  The  marsh  was divided into  twelve  cells,
each 6.1 m (20 ft.)  wide by 61 m (200 ft.) long  by 1.2  m (4
ft.) deep.  Non-chlorinated oxidation pond  effluent  was
pumped  to  a stilling tower  which  delivered  the  flow to  three
division  boxes  feeding four  cells  each.    Two operating
depths,  30.5  cm (12  in.)  and  61  cm  (24  in.),  and  four
theoretical hydraulic detention times  ranging  from 28  to 224
hours were used in the  project experiments.  Weirs controlled
                              25

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the  flows and  the depths  in the  cells.   The cells  were
planted  in alkali bulrush  (Sc i rpus  robustus) or  hardstem
bulrush  (Scirpus  acutus)  in the  spring  of  1981.   Numerous
wetland vegetation appeared  voluntarily, primarily cattails.

Table  2-1  summarizes  the  influent  and effluent quality data
for  the  Arcata system as a function  of  hydraulic  loading
rate.  The data presented in Table 2-1 represent the average
performance  of the  test systems  over a  two year  period.
Organic  loadings  of  166  kg/ha/day  (148  Ib/ac/day)   or  less
proved to be the most effective within the range of loadings
utilized.  BOD removal rates of 50% or greater were achieved
as long as dissolved  oxygen levels  remained  above  1.0 mg/L.
The best removal rates occurred during  the  fall and winter.
                          TABLE  2-1
           AVERAGE INFLUENT  AND  EFFLUENT VALUES FOR
                     ARCATA,  CALIFORNIA
                                 Effluent Concentration
                              Hydraulic Loading  (MGD/acre)
Concentration
BOD, mg/L 26
NH -N, mg/L. 12.8
pH, su 7.4
P04-P, mg/L . 6.28
0.06 0.12 0.24
10.7 13.3 12.8
9.6 11.6 9.8
6.6 6.6 6.5
6.05 6.17 7.0
Based upon data from September  1980 to September 1982
                              26

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Ammonia-nitrogen levels were effectively reduced  at loadings
of 0.1 to 2.5 kg/ha/day (0.09 to 2.24 Ibs/acre/day).  Ammonia
nitrogen made  up  approximately  80 percent of  the  total
Kjeldahl nitrogen in the marsh  effluent.   Over  70  percent  of
the organic  nitrogen  and 30 percent of the total  Kjeldahl
nitrogen was removed.   The higher  hydraulically  loaded  cells
produced lower concentrations of nitrates.  During periods  of
high dissolved oxygen,  nitrate  levels  increased from the
normal 1.0 mg/L to  3-4  mg/L.  Some denitrification did occur,
but was not a major factor.

Phosphorus was  only minimally removed, with  the  average
removal being five  percent.   The major  portion  of phosphorus
removal  occurred during  the  three to  four months  growing
season.  Phosphorus levels did  not noticeably increase during
non-growing seasons.

Non-filterable residues (NFR) were  reliably  removed  over  the
entire project period,  with  an  overall  average  of  85  percent
removal.   The  hydraulic loading  did not effect  the  removal
efficiency.  Removal efficiency did  increase as  the amount  of
vegetation increased  from  the beginning  of  the project.
Based  upon extrapolation of  pilot project data,  organic
loadings of 200 to  250  kg/ha/day (178 to  223 Ib/acre/day)  are
the maximum loadings   at which a marsh  treatment  system  can
produce an effluent low in NFR.
2.5  Summary

The literature indicates  that  advanced  secondary  to tertiary
quality effluent  can  be produced for advanced primary quality
municipal  wastewater  (BOD of 40  to  80 mg/L).    Secondary
                              27

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effluent quality can be produced from primary wastewater (BOD
of approximately 150 mg/L).  Total nitrogen removal decreases
as the  influent BOD concentration  increases,  probably  as  a
result  of  decreased  oxygen  levels limiting  nitrification.
Artificial wetlands are not effective for phosphorus removal.
Alum can be used, however, to increase phosphorus removal.

Virtually all of the literature  sources  discuss  treatment  of
municipal wastewaters.    Even where  primary effluents  were
treated,  the  strength of  those  effluents  was  significantly
less  than  the strength of  most  food processing  and  brewery
wastewaters.     The  evidence   strongly  suggests   that   a
substantial level of preapplication treatment may be required
for those higher strength wastes.
                              28

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                          SECTION 3
                      DESIGN PROCEDURES


3.1  Alternatives  Available

The objective of  this  section  is  to present  alternative
procedures  for  designing  an  artificial  wetlands  treatment
system for high strength wastewaters.   The  data  presented  in
Section 2  indicate that  preappl ication  treatment: would  be
required  in  order   to   use   artificial   wetlands   because
artificial wetlands alone  are  not  suitable  for  high  strength
wastewaters.   Very  limited  information on the  design  of
wetlands  treatment systems is available,  and  most of the
available  information  relates to the use  of  wetlands for
treating  municipal  wastewaters.     The   design  procedures
presented   herein   should,   therefore,   be  considered   as
preliminary  in  nature.    All  of   the   design  procedures
presented  herein  can  be  found  in the literature.   Even though
these methods have not been  validated,  these  methods are
presented  herein  to suggest options for future research  work.
One of  the  goals  of  any  co-operative  investigation  between
the U.S. and  China may  be to  eliminate or  validate  some  of
these  design methods.

Five general  design  procedures  are presented.  The  first
procedure  is  based  upon the  practical experience gained  at
the systems described  in Section 2.  The  second approach
utilizes  constituent  loading  rates,  primarily  BOD,  for
design. The third approach is  based  upon  the detention time
required  for  treatment.  A  first-order  plug-flow  kinetic
model  forms the basis of  the  fourth  approach.   Finally,  the
fifth  method involves  performing a water balance to determine
the surface area  required  to create a non-discharge system.
                             29

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For the  design  procedures  developed herein,  an attempt was
made  to  conduct a limited validation  of these  methods by
comparing  the results  obtained with  practical  guidelines
developed from experience.   The  pretreated effluent quality
presented in  Table 3-1  was selected so as  to be within the
range of operating data  from the  Listowel  and  Santee  systems.
Thus, one would  expect  a  valid  general  design procedure to
yield results similar to those found at Listowel  and Santee.
Detention time and organic  loading  rate  are used to compare
the design procedures with  the existing data.

The advantages and potential limitations associated with  each
system are presented  with the discussion of  each method.  For
each design example,  the wastewater  characteristics presented
in Table 3-1 have been used as a  typical food  processing
wastewater.
                          TABLE 3-1

             CHARACTERISTICS  OF A FOOD PROCESSING
             WASTEWATER USED  FOR DESIGN EXAMPLES
Parameter
(mg/L)
BOD
TSS
Total N
Influent Flow
a
Range
(mg/L)
<50 to >30,000
300-500
10-100

. b
Design
Value
100
50
25
1 MGD
       Effluent Flow
(3785  M /day)
    0.85 MGD
(3200  M3/day)
a) of raw wastewater;  b)  after pretreatment
                              30

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Table 3-2  presents  a  guide and checklist  for  the design of
aquatic  treatment  systems,  including  artificial wetlands.
Although  not  all  of  the  information   required  in   this
guideline is currently available,  the design  process outlined
herein summarizes the  factors  which should be considered in
designing artificial wetlands.

Other  approaches  could  be  used  for  the  design  of  an
artificial   wetland   including   an  oxygen   balance,   the
first-order  model  presented   by   Gearheart  et   al.  (1983,
Equation  2-5) ,  or adapting procedures  from other  forms of
aquatic  or  land  treatment  systems.     The   data  are  not
available to  support  these methods, or  the  methods require
parameters which would be  difficult to measure  or  estimate.

For  example,  Hammer and  Kadlec  (1983)  developed a  mass
transport approach for use in  the  design  of wetlands, and the
reference should  be  consulted  for a complete development of
this approach.  In order to use this approach, however,   five
constants  for  the  wetlands must be known  including  mass
transfer   coefficients.      These   constants   could    vary
significantly between wetlands, and  it  does  not appear   that
there is any means to estimate these constants at this time.
Thus, a further discussion of  this method  is  not presented.
3.2  Options for Preapplication Treatment

The  data presented in  Section  2  and  conversations  with
several other wetlands experts  all  indicate that artificial
wetlands  are not suitable for treatment of high  strength
wastewaters.   Data presented  by Gersberg  et al.  (1984a)
indicates  that  even  primary municipal  wastewater   (BOD-148
mg/L) can significantly stress  a wetland.   If wetlands are to
be   included   in   a   process   scheme   for   high   strength
                              31

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                           TABLE  3-2
         DESIGN GUIDE AND CHECKLIST FOR  AQUATIC  SYSTEMS
                    (Stowell,  et  al.,  1980)

"Preliminary Investigation

 1.    Survey sites suitable for an aquatic  system.

 2.    Review  and  summarize climatic  factors:   temperature,
      precipitation,  and  wind.

 3.    Survey local flora:   growth season, vegetative  structure
      through  winter,   and  potential  to   affect  aquatic
      environments.

 4.    Review   discharge    requirements:       are   seasonal
      requirements possible, if justified?

 5.    Review literature with emphasis  on type  of project being
      envisioned,    contaminant    removal   mechanisms,   and
      treatment performance and reliability.

 6.    Formulate preliminary aquatic  system design,  including
      possible use of conventional processes.

 7.    Analyze  cost-effectiveness of  alternative  wastewater
      treatment  processes: go   or  no-go  on  aquatic  system
      design.
                               32

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                     TABLE 3-2  (cont'd)
Aquatic System Design

1.   Itemize  expected  flows,  influent  concentrations,  and
     discharge requirements.

2.   Identify    contaminant   removal    mechanism(s)     and
     environment(s).      (Complete  this   analysis   for   each
     contaminant to be removed.)
     a.   Removal mechanism(s).
          1)    Identify  removal  mechanism(s)   operative  on
               contaminant of concern.
          2)    Summarize kinetics of removal mechanism(s) and
               factors  affecting the  kinetics:  contaminant
               concentration, temperature,  pH,  etc.
     b.   Aquatic-environment(s)    required   for    removal
          mechanism(s) to be operative.
          1)    List requirements for aquatic environment(s).
          2)    List  factors  affecting   these   requirements:
               climatic factors, organic loading  rate,  plant
               species, chemical and equipment  input,  etc.

3.    Conceptualize aquatic  process  units  (APU's)  necessary.
(Complete this analysis  for each contaminant to be  removed.)
                                                 the   aquatic
a.   Plant selection.
     1)    What  components  necessary  to
          environment will it provide?
     2)    Seasonality in the local environment?
     3)    Local diseases and  predators:   seasonal  or
          year round?
                         33

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                      TABLE 3-2 (cont'd)

          4)   Nuisance potential?

     b.   Chemical and equipment input.
          1)   What  components  necessary   to   the  aquatic
               environment will it provide?

          2)   Continuous, seasonal, or emergency use.

c.   Management to maintain aquatic environment (e.g.,
     harvesting).

d.   Rate  of  contaminant  removal  and   APU   surface  area
          requirements on a seasonal basis.

4.   Design aquatic system.

     a.   Integrate conceptual APU's into designed APU's.
          1)   Maximize    concomitant    treatment,    process
               performance and system reliabilty.
          2)   Minimize  redundant   aquatic  environments  and
               costs.

     b.   Complete  quantities  mass  balance  (in  terms  of
          removal  mechanism  kinetics)   for each  contaminant
          for each APU for the seasons  of the year.

     c.   Are the APU's mutually compatible?

     d.   Are the discharge requirements met?

     e.   What  measures   can   be   taken   to   increase   the
          reliability of the system?
                              34

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                      TABLE 3-2 (cont'd)
     f.   Management.
          1)    To maintain the system.
          2)    Courses of action in case  of  system  upset.
5.   Make  final  check  on  cost-effectiveness  of   aquatic
          system.

6.   Design and operate pilot-scale  facility  .   (This  step  is
          necessary until contaminant  removal mechanism  rates
          and  factors  affecting  these rates are quantified.
          If the pilot  does  not function as  anticipated,
          aquatic system design steps  2  and 6 will  have  to  be
          modified  as   necessary.  Failure   of   the   pilot
          facility  could  result   in  the  aquatic  system
          alternative  being  dropped  from  consideration  for
          the particular project.)

7.   Design and construct full-scale aquatic  system."
                              35

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wastewaters, then some form of preapplication treatment will
be required.

Stowell et al.  (1985) provide guidance and design parameters
for pretreatment options which can  be  considered  for use in
conjuction  with wetland treatment  systems.    These  include
Imhoff  tanks,   aerated  lagoons,  primary  sedimentation,  and
primary  filtration.    These   options,  however,  are  based
primarily for  the  treatment  of domestic  sewage,  and  the
applicability of these methods  to food  processing wastewaters
will  depend  upon   the   characteristics   of  the   specific
wastewater.   The design  parameters for  these pretreatment
methods  are presented in Table 3-3.  Wile et al. (1985)
recommend using a complete mix,  aerated lagoon  with  an 8 to
10  day   detention   time  for   pretreatment  of  municipal
wastewater.  For  a high  strength  wastewater, additional
detention time  may  be  required.   Other pretreatment options
include slow rate  (with  underdrains)  and  overland  flow land
treatment.
3.3 Practical Design Guidelines

The early design procedures for  other  forms of land treatment
systems were primarily based upon  practical experience gained
at  existing  systems.   For  municipal  wastewater,  hydraulic
loading  rates were  often used for design.   The  hydraulic
loading rate used successfully at one system could easily be
adapted for  use  at  other systems due  to  the relatively low
range of  variability in the concentrations  of raw municipal
wastewater.   The  range of wastewater characteristics within
the  food  processing industry,  however, is much  wider.   The
variability  of  wastewater  characteristics,  however,  will
largely be eliminated  by the  requirement  for   pretreatment
prior  to  the  wetlands.   If primary to  advanced  primary
                              36

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                          TABLE 3-3

         PRETREATMENT DESIGN PARAMETERS FOR USE WITH
       WETLAND TREATMENT SYSTEMS (Stowell et al., 1985)
Pretreatment
                                        Design Parameters
  Units
Value
Imhoff tank

  Overflow rate
  Detention time
  Sludge storage capacity

Lagoon

 Aerated
  Detention time
  Depth

 Facultative
  Detention time
  Depth
  BOD  loading

 Anaerobic

  Detention time
  Depth
  BOD  loading

Primary sedimendation

  Overflow rate
  Detention time
  Depth

Primary filtration

  Surface loading rate
gal/ftVd
    h
    mo
    d
    ft
    d
    ft
Ib/acre/d
    d
    ft
Ib/acre/d
gal/ftVd
    h
    ft
      2
gal/ft /min
 600
   3
   6
   5
  12
  10
   5-6
  50
  30
  14-16
 300
1000
   2
  12
                              37

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quality  wastewaters  are discharged to  the wetlands,  the
following guidelines  should be  applicable  for  designing  a
system  to  produce   secondary   quality   efflue;nt.     These
guidelines are based upon the research conducted by Black et
al.  (1981), Wile  et  al.   (1985),  Gersberg  et al.  (1983,
1984a,b), Stowell et al.  (1980),  Gearheart  et al.  (1983), and
Reed (pers.  comm.).

     Detention time  - Seven  to  ten  days.

     Hydraulic loading rate  - 2  to  20 cm/day,  use the low end
                              of    the   range   for   primary
                              wastewater.

     Water depth - <10 cm in summer,  >30 cm in winter.

     Physical   configuration -  a  length:width  ratio  >15:1,
                              channel width of  approximately
                              3 m for ease  of harvesting.

     Vegetation - Cattails  (Typha spp.)

     Organic  load ing  rate  - 15 to  120  kg BOD/ha/day,  use
                            lower values  of this range  for
                            primary wastewater,  and values in
                            the   middle  of  this  range  for
                            nitrogen  removal from  advanced
                            primary wastewater.

     Mosquito  control - required  at all but remote locations.

     Influent  structures  - multiple flow diffusers
                           recommended.

     Effluent  structures  -  collection manifold recommended.
                              38

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Design Procedures;

     1.    Calculate  the  wetland  volume  required  to achieve
          the desired  detention time;

          Seven days  was selected as  the design  detention
          time.

          V = (3785 m3/day)(7 days) = 26495 m3

     2.    Select   an  operating  depth   and   calculate  the
          required area.

          A depth  of  20 cm as  a  year-round  average  was
          selected.

          a = 26495/0.2  = 132000m2 = 0.029 m2 = 13.2 ha

     3.    Calculate  the  hydraulic loading  rate.

          Loading  rate =  3785m3/day/132000m2  =  O.,029m/day =
                         2.9 cm/day

          2.9 cm/day  is  within the acceptable range.

     4.    Calculate  the  organic loading  rate.

          mass of BOD  applied = 378.5 kg/day

          378.5/13.2  =  28.7  kg/ha/day,  which is  within  the
                       acceptable range.
                              39

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3.4  Limiting Constituent  Method

Other forms of land treatment  systems  (e.g., slow rate, rapid
infiltration)   are  often  designed   by   determining   the
acceptable mass  loading  of  organics,  nutrients,  and  trace
elements.  Then, by knowing the concentration  of each of the
parameters of concern in the  wastewater,  the acceptable
hydraulic loading rate associated with each parameter can be
determined.   The  hydraulic loading rate  is  selected either
based  upon  the  hydraulic loading   rate  of  the  limiting
constituent  or  the  infiltration  capacity  of  the  soils,
whichever is less.  The required  land  area  is then calculated
from the selected hydraulic loading rate.

A similar  approach  can be used  for the  design  of  wetlands.
Table  3-4  sumarizes  the  reported  removal  efficiencies  and
organic loading rates  reported in the  literature.  These data
were developed from systems where the  wastewater ranged from
primary  to secondary  (40 mg/L)  quality.   Gersberg  et  al.
(1984a)  found that nitrogen  removal  decreased as  the  BOD
concentration increased  even if the  BOD loading rate  was
roughly the same.  Thus, judgement  is  required when using the
data presented in Table 3-4.   Another  potential  problem with
the  data  presented  in Table 3-4 is  that the effect  of
operating parameters  such  as  water depth and  residence time
is unknown.
                              40

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                          TABLE 3-4
              LOADING RATES FOR WETLAND SYSTEMS
Parameter
BOD
Nitrogen
Loading Rates
(kg/ha/day)
18.4
77
88.2
106
116
6.04
44.4
2
Removal Efficiency
89%
90
93
78
86
86
79
(63.5 to 7 mg/L)
(40.9 to 41)
(49 to 3.2)
(148 to 33)
(76 to 10.7)
(25 to 8.3)
(24 to 5)
 Developed from data presented in Section 2.
2
 Numbers in parentheses represent reductions from influent to
 effluent.
The   following   design  example   is   presented   using   the
wastewater characteristics presented in Table 3-1.


     Design Procedures:

     Wastewater Characteristics (from Table 3-1).
            Q  =   106 gallons/day = 3.785 x 106 L
          BOD  =   100 mg/L
       Total N =    25 mg/L

1.   Select design wastewater loading rates


     The following values were  selected  based  upon judgement
     and the data presented in Table 3-4.
          BOD:     75 kg/ha/day
            N:      6 kg/ha/day
                              41

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Low BOD and nitrogen  loading  rates were selected  because  it
was  felt  that  the  high  BOD  concentration  could  create
anaerobic conditions which would inhibit nitrification.

2.  Calculate  mass loadings rates

          BOD: (3.785 x 106L/d)(100 mg/L)(10~6 kg/mg) =
               378.5 kg/day

      Total N: (3.785 x 106L/d) (25 mg/L) (10~6 kg/mg)  =
               94.6 kg/day

3.  Calculate  Required Surface Area

          BOD: (378.5 kg/day)/(75 kg/ha/day)  = 5 ha
      Total N: (  94.6 kg/day)/(6 kg/ha/day)  = 15.7 ha

Thus, approximately 16 ha of wetlands would be required.

4 .  Estimate treatment performance

The effluent quality can then be  estimated using  Figure  2-1,
or  Figure 3-1.    It  is important to  note,  however,  that
neither of these figures were developed  for  primary  strength
wastewaters.  The effluent BOD concentration can be estimated
directly   from   Figure   3-1,   which   yields  an   effluent
concentration  of approximately  30 mg/L.   This concentration
roughly agrees with  the  reduction of an influent  BOD of 148
to  33  mg/L at a  loading rate of  106 kg/ha/day  reported  by
Gersberg et al.  (1984a) .   Figure 2-1 indicates that 95% BOD
removal will be obtained at a loading  rate  which  corresponds
to  an  effluent  concentration of  approximately 5 mg/L.   The
latter procedure (Figure 2-1) probably  yields an  unrealistic
estimate of the effluent quality.
                              42

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5.    Select a water depth and calculate detention  time

Using a  water  depth of  20  cm yields  a  water  volume in  the
wetlands of 31,400 m .   The  theoretical  detention  time  would
thus be  approximately  8.3 days which  is within the optimum
range of  7 to 10  days  reported in  Section  3.3.   Since  the
same data base was used  to develop  this method  and  the method
described in Section 3.3, however,  the  results  should roughly
agree.
3.5  Detention Time

Stowell,  et  al.  (1985)  presented design  curves  for  sizing
wetland  systems  to produce  secondary  quality wastewater  by
treating  raw,  primary,  and  advanced municipal wastewaters,
but not high strength wastewater (Figure 3-2).   This  curve is
also limited for  use  at  a loading rate of 100  Ib  BOD/ha/day
and a water depth of one foot (30 cm).   No data are presented
to support this  design curve.   This method is  presented  for
information purposes  only  and  is not recommended  for  design
use until supportive data are available.

     Design Procedures;

     1.  Estimate the critical  wetlands temperature

For the  detention times  generally encountered  in  artificial
wetlands,  the  water temperature should  be  roughly  equal  to
the design air  temperature,   to  a minimum  of 32  F.   The
average air temperature during  the critical  time of year
                              44

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    0
   10
o
0
J_
13
•+-•
03
0)
Q.
E
15
   20 -
  25  —
  30
         BOD  Loading Rate = 100 Ib/acre.d
         Depth = 1.0 ft
         »= 1.10
                                                    Decreasing Temperature
 Untreated Wastewater
 BODs=220 mg/L
                              •Primary Effluent
                              BODs= 145 mg/L
                        -Advanced Primary
                         BODs = 80 mg/L
                'Increasing Pretreatment
              i   I  i  i   i  i  I  i   i  i  i
                                                                      i  i
     0
                         10
15
20
25
30
     Figure  3-2.  Detention Time  Required  to Achieve  Secondary
                   Quality Effluent (Stowell, et al.,  1985).
                                     45

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should be  used.   A  second  possibility  would be  to  use  the
temperature of nearby streams or wetlands.  A temperature of
10 C was selected for this example.

     2.  Determine the detention time

Figure  3-2  presents  a  relationship between  the wastewater
temperature and the required detention  time for  three  levels
of  pretreatment  (none,  primary,  and   advanced  primary).
By interpolating between the curves,  the detention times
required for  treatment  of other influent BOD concentrations
can be found.

Using the temperature determined in  step  1,  use  Figure  3-2 to
determine the required detention time.  At 10°C,  the  required
detention  time  to  produce  secondary quality  effluent is
approximately 8  days.   This detention time agrees  with  the
recommendations presented  in Section 3.3.

     3.  Determine the land area required

          A = Qt/d

where,

                             2
          A = surface area, m
          Q = influent flow rate, m  /d
          t = detention time, d
          d = water  depth, m

     A = (3.785 x 106 L/d)(8 d)(10~3m3/L)/O.3m  =
          1.01 x 105m2
          A = 10 ha.
                              46

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This surface  area  yields  a BOD  load  rate of  38  kg/ha/day,
which is in the acceptable  range.

Several problems exist with this method.   First,  one  cannot
specify  a  desired effluent  quality and  design the  system
accordingly.   Second,  one is limited to a loading rate  of 112
kg/ha/day  (100  Ibs/acre/day) ,   as   stated  on  the  design
curves.  Finally,  too  many  parameters may be specified  on the
design curves.  For example, the design curve specifies a BOD
loading rate of 100 Ib/acre/day  (11 kg/ha/day).   The  result
of  this  design  example  indicates  a  loading rate  of  34
Ib/acre/day (38 kg/ha/day).   This  design procedure  thus  does
not appear to be consistent.  The primary  advantage of  this
method  is that  temperature  can be  included  as  a  design
parameter,   and   temperature   fluctuations   may   have   a
significant effect  on  performance.
3.6  Plug-Flow First-Order Design Procedure

To  some  extent, especially for  shallow water  depths,  an
artificial wetland  system is analogous  to an  overland  flow
land treatment  system.    First-order  plug-flow models  have
been successfully used  to  describe  pollutant  removal  in  both
overland   flow  systems  (Abernathy,  et  al.,  1985;  Smith,  et
al., 1983;  Martel, et al.,  1982)  and  artificial wetlands
(Lakshman, 1980; Gearheart,  et  al.,  1983).   In  overland  flow
systems,  both slope and detention  time  have  been used  as  the
independent variables.   Slope length has  yielded  better
results  to  date due  perhaps  to  the inability  to predict
detention   time  on   an   overland  flow  slope   accurately
(Abernathy et al. 1985).   Sufficient data are  not  available
to  determine the  kinetic removal  rates as a function  of
distance   in  a  wetland.   Furthermore,  detention  time on  an
overland   flow  slope  is controlled primarily by  application
                             47

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rate and slope.   In a wetlands,  however,  detention time is
significantly affected by  water  depth.   Detention  time may
thus be a more  suitable  basis  for using a kinetic model for
wetlands design than the  length of the  wetland.

The design model to use would be  of the following  form:

     ln(C/Co) = -kt                                 [Eq.  2-4]

     where C, Co,  k and t are defined previously.

The potential  advantage  of using  this  approach  is  that the
detention  time required  to  achieve  a specified  effluent
concentration can be calculated if C,  Co, and k are known or
specified.

     t = -(l/k)ln(C/Co)                              [Eq.  3-2]

Values for the decay constant, k,  for wetlands are scarce to
non-existent.   Lakshman  (1980)  reported BOD  decay  rates of
0.14  to  0.25  per  day for wetlands treating influent BOD
concentrations of  30 to 100 mg/L.   The  data  from Listowel and
Santee  indicates  a  range of BOD  decay rates of from 0.25
(cold climate)  to  1.51  per day  (warm  climate).   A nitrogen
decay rate of 0.12/day was  found  to describe  the  performance
of the Listowel system.  Lakshman  (1980) reported that  total
nitrogen decay rates were  approximately one-third of the BOD
decay rates for an AWT wetlands.

BOD removal will occur both by solids  settling and microbial
oxidation.  If  a significant portion of  the influent  BOD is
associated with solids,  then two  decay  rates (one  each for
solid and  soluble  BOD)  may be needed.   Such data, however,
are not currently available.
                              48

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     Design Example

Suppose that  the  influent  wastewater  has the characteristics
presented in  Table  3-1  and  that  an  effluent BOD and nitrogen
quality of  30 mg/L  and 10  mg/L,  respectively,  is  desired.
The  following procedure could  be  used  to  size  the  wetland
system.

     1.  Select a BOD and nitrogen decay rate

It has been assumed  that  the BOD decay rate will be 0.25/day
and that the  nitrogen decay  rate will be 0.12/day, based upon
data presented  in Section 2.   The  lowest  values  found were
used to be  conservative.   If the wetlands  water temperature
will be significantly different  than 20°C, the BOD decay rate
can be adjusted using the following relationship:

                   T-20
     KT = (K2Qo)  9                                 [Eq> 3_2]
where

     K  = reaction rate constant at wetlands temperature ( C)
          in days

   K,, o = reaction rate constant @ 20 C.

      9 = temperature coefficient,  1.085
                               o
      T = water temperature  in  C.

Stowell et al.  (1985) used  a temperature coefficient  of 1.1,
which is close to the value of 1.085 used above.
                              49

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     2.  Estimate the required detention  time

Using Equation 3-2,

     for BOD removal:

     t = (1/k)(lnC/Co)=-(l/0.25)(In 30/100)  =  4.8  days

     for N removal:

     t = (1/0.13)ln(10/25)  = 8 days

Thus,   nitrogen    removal   will    control   the   land   area
requirements.

     3.  Calculate the  volume of  water  required  to  achieve
         the desired  detention time

From Table 3-1,  the influent flow rate is 3,785  m  /day.

The required water volume is thus:

     V = (8 days) (3785)  = 30,280  m3

     4.  Select  a design water depth

A  water depth  of 20  cm was selected  to promote  contact
between the soil and  wastewater.

     5.  Determine the required surface area

     A = V/d = 30,280 m3/0.2m = 15.1 ha
                              50

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     6.  Compute organic loading  rate

The influent mass of  BOD  to  the  system,  from design example
1, was 378.5 kg/day.   The  organic loading  rate is thus:

     378.5 kg BOD/day/15.lha  =  25 kg/ha/day

The results of  this  method  also  appear  reasonable  when
compared to the practical  guidelines.
3.7  Non-Discharge Design

All of the previous design  approaches assume treatment is the
desired   objective   of   the  wetlands   system.     However,
wastewater disposal  or  volume  reduction may be  the desired
goal.   If  so,  a  hydrologic  balance  can be  prepared  to
calculate the required surface  area.

It has  been assumed  that  water  losses  due  to  infiltration
will  be insignificant in accordance with the definition of a
wetland (see Section  1.4).   Without infiltration,  the water
balance becomes  simpler:

     change in  water volume = Wastewater  inflow +
                             precipitation - evaporation

Obviously,  for the system  to  be  non-discharging  without
infiltration occurring, evaporation  must exceed the  sum  of
precipitaton and wastewater inflow.
                              51

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     1.   Perform a water  balance

Using either  evapotranspiration  and  precipitation data  or
estimating evaporation from  empirical  formulas, determine the
amount  of water  lost per day  per  unit  area.   EPA  (1981)
presents methods for  calculating  a water  balance.   For this
example, assume that  25  cm  (10  inches)  has  been  selected as
the design evapotranspiration  rate.

     2.   Calculate the land  area  required

The volume of wastewater  generated each  year is:

     V = 3.785 x 102m3/d(365 days) =  1.38  x 105m3/year

The amount of water  evaporated  per  square  meter  is,  from
             3  2
above, 0.25 m /m /year.

The land area required is thus:

     A =  (1.38 x 106m3/yr)/(0.25  m3/m2/yr) = 55.3 ha

     3.   Calculate the organic loading rate

From Section 3.3, the BOD mass loading rate is:

     378.5/55.3 = 6.8 kg  BOD/ha/day

This value is well below  the loading  rates presented in Table
3-2.  Thus,  it  may be  more  economical  to  add additional
wetland capacity  for polishing  (if  a  discharge  of  higher
quality  effluent is feasible)  or to use another form of land
treatment.
                              52

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3.8  Summary of Design  Methods

All of the design methods yielded reasonable results.    The
practical design guidelines  (Section  3.3)  is the recommended
method  until the  other methods  can be validated.   These
practical  guidelines  were   developed   from  the  operating
experience  at several  systems  which have  performed  well.
The  other  more  rational   design procedures   need  to   be
subjected to a more  rigorous validation procedure.   One goal
of  a  future  research  project  may  be  to  conduct such  a
validation.   Additional research considerations  are outlined
in Section 5.
                              53

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                          SECTION 4
                      RELATED PROCESSES


4.1  Introduction

Two  processes  somewhat  similar  to  artificial  wetlands are
briefly discussed in this section.   These processes are the
Root Zone Method  (RZM)  and the  Rock/Reed Filter.   In the
purest  sense, the RZM is not an  artificial  wetlands since the
wastewater is maintained below the ground  surface.   The data
from operational systems in Europe indicate that this method
could  be effective for the  treatment of food processing
wastewaters.    A  discussion of this  method  is  presented
herein.    For more  detailed  design  information,  the reader
should  refer to Boon (1985)  and  Lawson  (1985) .

A process  similar  to the RZM is  the  Rock/Reed Filter  (RRF;
Wolverton, 1981, 1986).   Wolverton  describes  the  RRF as an
anaerobic filter  and  not as  a wetland.   This  process,
however, may also have some applicability  to a high strength
food processing wastewater  treatment scheme.  A discussion of
the RRF is also presented herein.
4.2  The Root Zone Method (RZM)

     4.2.1  Process Description

The  RZM is  a  subsurface treatment  system  where wastewater
flows through the root zone of a reed  (Phragmites) bed.  The
reed roots  serve  a number  of  functions.    First,  the roots
increase the  permeability  of the  soil.   Second,  the reeds
tranport oxygen from the  atmosphere  to  the roots  and into the
                              54

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root  zone.   The  RZM  is thus  an  aerobic  and  anaerobic
treatment process.   Nitrification occurs in the aerobic zone
around  the   root,   while denitrification   occurs  in  the
remaining  anaerobic  areas.     Phosphorus removal  occurs  by
soil adsorption and chemical  precipitation;  although, as with
wetlands,  the  phosphorus removal  cabability  may  eventually
become exhausted.    Higher  influent  BOD concentrations  (on
the order  of  600 mg/L)  can be treated by this method due to
the more  intimate contact  between  the wastewater  and the
microorganisms.  Three  years  are required,  however,  for the
roots to create sufficient soil  permeability for treatment to
occur.  Design  considerations for  this method are presented
below.
     4.2.2  Design Procedures

The following design steps for the RZM are presented by Boon
(1985) :

    "1.  Selection of a Site

     The site should be  an open  ground not shadowed by
     trees or shrubs.    The  slope of the ground  should
     not be excessive; between 2 and 6 percent would be
     acceptable.   If possible,  the soil  covering the
     site (up to a depth of  at least 1.5 m) should  have
     a hydraulic conductivity of  about  10    to  10    m/s.
     A 'light1 clay or a 'heavy1  top soil would be best;
     such  soil  could   be   brought  to  the  site  if
     necessary.
                              55

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2.  Preparation of a Site

Existing soil has to be removed  to  a depth of about
1.5 m  below the level of  incoming  sewage  so that
sewage  will gravitate  through the reed bed.    A
sealant of  clay  (with  a  hydraulic  conductivity of
less than  10~°  m/s),  bentonite, synthetic  fabric,
or  asphalt  is  required  to  retain  water  in  the
reed-bed and prevent contamination  of ground water.
After sealing the site,  the soil  in which the reeds
will be planted has to  be  replaced.   A  'light'
clay, containing some quartz and  chalk would be
ideal.    This soil should be carefully placed to a
depth of  about 0.6 m, avoiding the use of  heavy
vehicles which might compress  the  soil  and reduce
its hydraulic conductivity.

At the  inlet and outlet ends of each bed,  trenches
should  be left into which stones, granite,  or slag
can be  placed.    The nominal diameter of the medium
(similar to that used  for biological filters)  would
be  60  to 100  mm.   Each trench should be  sited
across  the width of a bed and should be about 0.35
m wide  and  0.65  m deep.  Final  effluent  would be
collected into  the outlet trench  and flow out via a
20 cm diameter  pipe,  the discharge level  of  which
should  be capable  of  variation.   During normal
operation, the level of  water  would  be maintained
at about the top of the  bed or  just below (1-2 cm).
                         56

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3.  Design Details for a RZM Works
The width of a bed would depend upon the slope from
inlet to  outlet.     The cross-sectional  area
can be calculated from the equation.
                                               (A )
Ac - 0
Ac " °-
where
                                             -  4~1]
         kf dH/ds
          is the average flow-rate of sewage (m /s)
                                            32
          is the  hydraulic conductivity  (m /m   s)
          of the  fully  developed  rhizosphere  (i.e.
          k
                           - 3  3  2
             is equal to 10   m /m  s) ,  and
  dH/ds   is the slope of the bed (m/m).

This  equation  applies  to   a   RZM  bed  treating
effluent,  raw  or  settled  sewage,  or  a readily
biodegradable wastewater with a bed depth about 0.6
to 0.65 m.    For wastewaters which are difficult to
biodegrade, the depth may have to be reduced  to 0.3
m.
     The area of a bed  (A, )  can  be  calculated  from
                          n
the equation.
      ^ = 5.2 Q0 (In.C -In.C )
      n        dot
                                    (m)  [Eq. 4-2]
                         57

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          where
          Q,   is the average flow-rate of  sewage  {m /d)
          C    is  the average  BOD  of the  wastewater
               (rag/L) ,  and
               is  the
               (mg/L) .
C    is  the  average  BOD  of  the  effluent
     The value  of 5.2  relates to  the  removal of  BOD from
     sewage in the bed which is to  be  0.6 m deep and operated
     at a minimum temperature of 8°C.   For more difficult to
     biodegrade wastewaters and lower temperatures the value
     will be greater,  up to 14  or  15."
     Design Example

Using the wastewater characteristics presented in Table 3-1/
one can calculate the land  area  required  for  an RZM  treatment
system using Equation 4-2,  as follows:

          A,  = 5.2 (378.5)  (In 100  - In 30) = 2370 m2
           n
               A,  = 0.23 ha.

This method  thus  obviously requires less  land  area than an
artificial wetland for the  same  wastewater.
     4.2.3  Operating Guidance

Three years are  required  for  an RZM  system  to become fully
operational.   During  the  first year,  secondary  or  higher
quality effluent should be applied.  During the second year,
raw  (screened and degritted) wastewater  should gradually be
added  with the  effluent.   At  first,  a  proportion  of 30
                             58

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percent raw  municipal' wastewater to  70  percent effluent  is
recommended.    Gradually,  the  percentage  of  raw  wastewater
should be increased until  by  the end of the third year,  raw
wastewater may be treated.

Harvesting should not be conducted as cutting the  reeds  will
reduce  the   transport   of  oxygen   into   the   root   zone.
Additional design,  construction,  and operating  guidance  can
be found in Boon (1985)  and Lawson (1985) .
4.3  Rock/Reed Filter (RRF)

     4.3.1  Process Description

The RRF  filter  is essentially a horizontal  trickling  filter
into  which  reeds,  rushes,  or  similar  plants   have  been
planted.  Wastewater enters the filter after  pretreatment  to
remove  solids and decrease the  organic loading  and flows
through  a  rock  bed.    The  bed media  consists  of   a  one-foot
layer of  4-inch  rock overlain by  4  inches  of topsoil.   The
length to width ratio of the bed is typically 50:1.

A microbial film  grows on the rock surfaces  similar  to  a
trickling filter.   As the wastewater passes through  the  bed,
this microbial film  purifies  the  wastewater.   Influent BOD
concentrations of  approximately 120  mg/L  have been  treated
with this method.  With a 24-hour  detention time,  an  influent
BOD concentration  of 116 mg/L was  reduced  to  12 mg/L  (90%
reduction).   Wolverton  (1982,  1986)   should  be  consulted for
additional information on the performance of this  system.
                              59

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     4.3.2  Design Information

Very limited design  information is  available on  the RRF.
Important design  parameters  identified  by  Wolverton  (1986)
are:  rock sizes, retention time, and vegetation type.  From
the literature, it appears that  a four-inch  rock  size and a
24-hour detention time are  optimal.   The  vegetation type will
depend  upon  the climate.    A  hydraulic loading   rate  of
approximately 9 inches/day  (23  cm/day)  for primary wastewater
is also suggested.   At this  loading  rate, approximately 2 ha
would be  required to treat the  wastewater  characterized  in
Table 3-1.
                              60

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                          SECTION 5
                   -RESEARCH CONSIDERATIONS

5.1  General

The  results  of  this  investigation indicate  that  there  are
currently no widely  accepted  rational  design procedures  for
artificial wetlands.    Furthermore, examples  of  the use  of
wetlands for further  treatment  of high strength wastewaters
were not  found in the  literature.  Therefore, many of  the
design procedures presented in Section  3 may not: be valid  for
the design  of  artifical  wetlands for treating high  strength
wastewaters.

The  literature data  that  was collected  appear  to  indicate
that wetlands  treatment  technology is  not suitable  for high
strength wastewaters.  Testing  of this technology,  at  least
on a  pilot-scale,  however, should  perhaps  be considered  to
further  evaluate  this technology.    A  possible  research
program is outlined below.
5.2  Economic Evaluation

Before investigation in a field research program,  an  economic
evaluation of  using  wetlands  for  treating  food  processing
wastewaters should be considered.    This treatment technology
can perform adequately providing a low  BOD mass  loading  rate
(kg-BOD/ha/year)  is used.    The  lower  the loading rate,
however, the greater  the  land  area required, and  hence,  the
greater  the  cost.   The cost  of  pretreatment must  also be
considered.   The purpose of the economic evaluation  would be
to determine, based upon currently available  information,  the
range of loading rates and  perhaps climatic  conditions  under
                              61

-------
which wetlands  treatment  might be  more cost-effective than
other forms  of conventional treatment or land  treatment,
including the  costs  of pretreatment  in the  analysis.   Any
field research program could then  focus on these  loading
rates and other conditions.   If factors other than economics
are  important,  such  as wildlife habitat,  then  the economic
evaluation may not  be the  determining  criteria.
5.3  Field Research Program

The next  phase of a research program  would be  to  conduct
pilot-scale  testing   of   wetlands   under   the  conditions
identified  as  being   potentially  cost-effective   in  the
research program to permit an  accurate determination of:

     o    a hydrologic  budget,
     o    an oxygen balance
     o    hydraulic detention  time, and
     o    treatment performance
          - through the wetlands,
          - influent vs. effluent  quality.

The pilot system(s)  should have  the capability to  permit:

     o    variation of  the hydraulic loading  rate,
     o    variation of  the organic loading rate,
     o    recycle of the effluent,
     o    collection of soil water and ground water samples
          beneath the wetlands,  and
     o    variations in the  influent distribution  system.

All of  the  above  information  and   process capabilities would
be useful in evaluating the  design  procedures presented here
and, if necessary for development  of new procedures.
                              62

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                          SECTION 6

                         BIBLIOGRAPHY
Abernathy,  A.  R.,  J.  Zirschky,  and  M.  B.  Borup,  1985.
     "Overland  Flow  Wastewater  Treatment  at  Easley,  S.C."
     Journal	Water	Pollution	Control	Federation.
     57(4) :291-299.

Bender, M.  E. and D. L. Correll. 1974.  "The Use  of  Wetlands
     as  Nutrient  Removal   Systems."    Chesapeake  Research
     Consortium Pub.  No.  29.   University of Maryland, College
     Park,  MD.

Bevis,  F.   B.  1981.    Reuse  of   Municipal   Wastewater   by
     Volunteer   Fresh-Water   Wetlands  (Vermontville,  MI) .
     PreparedforNationalScienceFoundation,Washington,
     D.C.,  Williams and Works, Grand  Rapids, MI.

Black, S.  A., I. Wile,  and  G. Miller.  1981. "Sewage  Effluent
     Treatment in an  Artificial  Marshland."   1981  Water
     Pollution Control  Federation Annual Conference,  Detroit,
     Michigan.

Boon, A. G.  1985.  Report of  a Visit  by Members and  Staff  of
     WRc to Germany  to  Investigate  the  Root Zone  Method  for
     Treatment  of  Wastewaters,  WRc  Processes,  Herts,  United
     Kingdom,63p.

Boyt, F. L., S. Bayley, and J. Zoltek, Jr.  1977.   "Removal  of
     Nutrients  from  Treated  Municipal Wastewater  by Wetland
     Vegetation."      Journal   Water    Pollution  Control
     Federation, 49:789-799.

Chan, E.,  T. A. Bursztynsky,  N.  Hantzsche,  and Y.  J. Litwin.
     1981.   The Use  of Wetlands  for Water  Pollution  Control.
     EPA-6 00/2-8 2-086,   U~.    S~.   EPA  Municipal   Engineering
     Research Laboratory, Cincinnati,  OH.

Conn, W. M.  and A.  C. Langworthy. 1984.   "Practical Operation
     of a Small Scale Aquaculture."   Proceedings  Water Reuse
     Symposium III, San Diego, CA., American Water  Works
     Association, Denver, CO, pp.703-712.

Cornwell,  D. A., J. Zoltek, C. D. Patinely, T.  D.  Furman,  and
     J. I.  Kim. 1977.  "Nutrient Removal  by Waterhyacinths."
     Journal of Water Pollution Control  Federation, 49:57-65.
                              63

-------
DeBusk,  T.  A.  and J.  H.  Ryther.  1984.   "Nutrient Removal from
     Domestic  Wastewater by  Waterhyacinths:   The  Importance
     of     Plant  .  Growth,    Detritus    Production    and
     Denitrification."    Proceedings  Water  Reuse  Symposium
     111, San  Diego,  CA, American  Water  Works Association,
     Denver, CO, pp.713-722.
Demgen,  F.  C.  1979.   "Wetlands Creation for Habitat and
                                                         i a."
                                                         Lnar
                                                         EPA
en, r.  v_.  iy/y.    wetxanas  creation  IOL  naDitat  ana
 Treatment  at Mt.  View Sanitary  District,  California."
                                                 Seminar
     Treatment at  Mt.  View  Sanitary  District, Califc
     Aquaculture  Systems  for Wastewater  Treatment:   i
     "Proceedings     and    Engineering    Assessment,
     430/9-80-006,  U.S.  EPA,  Washington,  DC,  pp.61-73.

Demgen,  F.  C. 1984.  "An  Overview of  Four New  Wastewater
     Wetlands Projects."   Proceedings  Water Reuse Symposium
     III, San Diego,  CA, American Water Works Association,
     Denver, CO,  pp.579-595.

Dempsey, H.  H. and  J.  E.  Shelton.  1983.   "Advanced Biological
     Treatment of Municipal Wastewater Through  Aquaculture."
     EPA-660/S2-83-007,  U.  S.  EPA,  Cincinnati,  OH.

Denny.   1972.   "Sites  of  Nutrient Absorption  in  Aquatic
     Macrophytes."   Journal  of Ecology. 60:819.

Dinges, W.  R. 1978.   "Upgrading  Stabilizaion  Pond  Effluent by
     Water   Hyacinth  Culture,"   Journal  of  Water Pollution
     Control Federation,  50:833.

Dinges,  W.  R. 1979.   "Development of  Hyacinth  Wastewater
     Treatment Systems  in  Texas."  Aquaculture Systems for
     Wastewater    Treatment;    Seminar    Proceedings   and
     Engineering  Assessment,  EPA-430/9-80-006,  pp.193-231.

Duffer, W.   R. and  J  E.  Moyer. 1978.   Municipal Wastewater
     Aquaculture.  EPA-600/2-78/110, U.  S.   EPA,  Cincinnati,
     OH.

Engler,  R.M.,  D.A.   Antic,   and   W.H.   Patrick,  Jr.   1976.
     "Effect  of  Dissolved Oxygen on Redox  Potential and
     Nitrate  Removal  in Flooded  Swamp and Marsh  Soils."
     Journal of  Environmental Quality, 5^:230-235.

Ewel,  K.  C.  and H.  T.  Odum.  1978.   "Cypress  Swamps for
     Nutrient Removal."   Advances  in  Water  and Wastewater
     Treatment,  Biological  Nutrient Removal.  M D. Wanielista
     and W.  W. Eckenfelder  (eds.),  pp.181-198.

Fetter, C.  W. , 1978.   "Use of a Natural  Marsh  for Wastewater
     Polishing."   Journal Water  Pollution Control  Federation,
     50:290-307.
                              64

-------
Gaigher, I. G.,  D.  F.
     "Preliminary Studies
     Brewery      -Effluent       in
     Bacterial/Algal/Fish/Macrophyte
     Agricultural Wastes, 12:207-224.

Gearheart,  R.  A., S,
     and S. Sunberg  	
     Marsh   Pilot Project
            and Management
            Arcata,  CA
                   Toerien,  and J.  U.  Grobbelaar. 1985.
                      on  the  Treatment of  Sorghum Beer
                             in      an       Integrated
                                    Culture     System."
 Design
 Works,
   .  Wilbur/  J.  Williams,  D.  Hull,  B.  Finney,
   j.  1983.   "Final Report:   City of  Arcata
   Project  Effluent  Quality  Results—System
   agement."   City of  Arcata,  Dept.  of Public
Gearheart, R. A., B. Finney,  S.  Wilbur,  J.  Williams, and D.
     Hull. 1984.  "The Use of Wetland Treatment Processes in
     Water Reuse."   Proceedings Water  Reuse Symposium  III,
     San Diego,  CA,  American Water  Works  Association,  Denver,
     CO, pp.617-638.
Gee
and Jenson
 Treatment
Engineers.  1980.
 Design   Manual
Water  Hyacinth  Wastewater
     Laboratories,   NSTL
     Engineers, West Palm

Gersberg,  R.  M., B. V.
     "Nitrogen  Removal
     Research, 17:1009.
                      Station,
                     Beach, FL.
     Prepared    for
  MS.     Gee  and
                    Elkins,  and C.
                     in  Artificial
                         R. Goldman.
                         Wetlands."
  NASA
Jenson
                    1982.
                    Water
Gersberg, R.  M.,  B.  V. Elkins,  and  C. R.  Goldman.    1984a.
     Wastewater Treatment  of  Artificial  Wetlands."   Water
     Science and Technology,  17:443-450.
Gersberg, R.  M. ,  B.  V. Elkins,  and  C.
     "Use of  Artificial  Wetlands to
                "       Journal   Water
 Wastewater.
 Federation,
                 56:152-156.
                         R.  Goldman.    1984b.
                       Remove  Nitrogen from
                          Pollution    Control
Gersberg,  R.  M.,  B.  V.  Elkins,  S.  R. Lyons,  and  C.  R.
     Goldman.   1984c.    "The Removal  of  Heavy Metals  by
     Artificial Wetlands."  Proceedings  Water  Reuse  Symposium
     III, San  Diego,  CA,  American  Water Works Association,
     Denver,  CO, pp.639-648.
Gersberg,  R.  M.,  B.  V.  Elkins,  S.  R.  Lynch,
     Goldman.  1985.   "Role  of Higher Aquatic
     Wastewater Treatment by  Artificial Wetlands."
     manuscript.
                                              and C.  R.
                                              Plants  in
                                                 Unpubl.
Godfrey, P.  J.,  E. R.  Kaynor,  S.  Pelczarsk,  J.  Benforado.
     1985.    Ecological  Considerations  in Wetlands  Treatment
     of Municipal  Wastewaters"!Van  Nostrand  Reinhold,  New
     York.
                              65

-------
Hall, D.  H.  and J.  E.  Shelton.  1983.   Advanced Biological
     Treatment of  Municipal  Wastewater  Through  Aquaculture.
     EPA-600/2-83-007,  U.  S.  EPA, Cincinnati, OH.

Hammer,  D. E. and R.  H.  Kadlec. 1983.    Design  Principles for
     Wetland Treatment  Systems.  EPA-600/2-83-026, U. S. EPA,
     Cincinnati, OH.

Hantzche,  N.  N.  1985.   "Wetland  Systems  for  Wastewater
     Treatment:  Engineering  Applications."   Proceedings of
     Symposium  on  Ecological  Considerations  in  Wetlands"
     Treatment of Municipal Wastewater.   P. J.  Godfrey et al.
     (eds.)  Van Nostrand Reinhold, New York, pp.7-25

Hardisky, M. A., R.  M.  Smart, and V.  Klemas.  1983.   "Growth
     Response  and   Spectral  Characteristics   of  a   Short
     Spartina   Alterniflora   Salt   Marsh  Irrigated   with
     Freshwater and  Sewage  Effluent."   Remote  Sensing of
     Environment, 13:57.

Hauser,  J.   R.   1984.   "Use  of  Water  Hyacinth  Aquatic
     Treatment  Systems   for   Ammonia  Control   and  Effluent
     Polishing."   Journal of the Water Pollution  Control
     Federation, 5j5(3) :219.

Heliotis,  F.  D.  1982.   Wetland  Systems  for  Wastewater
     Treatment;   Operating  Mechanisms  and  Implications for
     Design.   IES  Report #117, Institute  for  Environmental
     Studies, University of Wisconsin, Madison,  WI.

Herskowitz,  J.  Water Resources  Branch,   Ontario Ministry of
     the Environment, personal communication.

Huggins, A.  Environmental Scientist, Environmental Resources
     Managment,    Inc.,   West    Chester,    PA,   personal
     communication.

Hyde, H.  C. ,  R.  S. Ross, and F. Demgen.   1984.  Technology
     Assessment  of   Wetlands   for  Municipal  Wastewater
     Treatment.    EPA-600/2-84-154,  U.   S.  EPA,  Cincinnati,
     urn

Jewell,  W.  J.,  J.  J. Madros,  W. W.  Clarkson,  H. Pompe,  and
     R.  M. Kabrick. 1983.  "Wastewater  Treatment with  Plants
     in  Nutrient  Films."   EPA-600/S2-83-067, U.  S.  EPA.
     Cincinnati, OH.

Kaczynski, V. W. 1985.   "Considerations  for Wetland Treatment
     of Spent  Geothermal  Fluids."   Proceedings of Symposium
     on  Ecological  Considerations of  Wetlands  of  Muncipal
     Wastewater, P.  J.   Godfrey, et  al.   (eds.), Van Nostrand
     Reinhold, New York, PP.48-68

                              66

-------
Kadlec, R. H., et al. 1981.   "The  Hydrology  of  Overland  Flow
     in  Wetlands."    Chemical   Engineering  Communications
     2:331.

Kadlec,  R. H., C.  J.  Richardson,  and J. A.  Kadlec. 1975.
     "The Effects of Sewage  Effluent on  Wetland Ecosystems."
     Semi-Annual  Report  No.   4.,  NTIS  PB249192,  Washington,
     D.C.  University of Michigan,  Ann  Arbor.

Kadlec, R. H.,  D.  L. Tilton,  B.  R.  Schwegler, and Benedict  R.
     1979.  "Three-year Summary of  Pilot  Scale  Operations  of
     Houghton  Lake."     Report   to  the  National   Science
     Foundation, NTIS PB295965,  Washington,  D.C.

Kadlec, R. A. and D. L Tilton. 1979.   "The Use  of Freshwater
     Wetlands   as    a    Tertiary   Wastewater    Treatment
     Alternative."   CRC  Critical   Reviews   in  Environmental
     Control, 2(2):185.

Kadlec,  R. H., 1979.   "Wetlands  for  Tertiary Treatment."
     Wetland  Functions  and  Values:     The  State   of  Our
     Understanding.    American Water  Resources  Association,
     Minneapolis,  MN, pp.490-504.

Kadlec, R. H.,  1985.  "Wetlands  Utilization  for  Management  of
     Community Wastewater."  1984  Operations  Summary,  Wetland
     Ecosystem    Research    Group   International    Report,
     University of Michigan,  Ann  Arbor.

Kadlec, R. H. and  D. E.  Hammer.  1984.   "Wastewater Renovation
     in Wetlands:   Six Years at Houghton  Lake."   Proceedings
     Water Reuse Symposium III,  San Diego, CA,  American Water
     Works Association,  Denver,  CO, pp.596-616.

Khettry,  R.  K. 1984.   "The  Use of Artificial Marshes for
     Wastewater  Treatment  in  Ontario,   Canada."    Ontario
     Ministry of Environment, Toronto,  Ontario,  Canada.

Lakshman, G.  1979.  "An Ecosystem  approach  to  the  Treatment
     of Waste Waters."   Journal  of Environmental Quality,  8
     (3): 353-361.

Lakshman,  G. 1980.   A Demonstration Project at  Humboldt  to
     Provide Tertiary  Treatment  to the  Municipal  Effluent
     Using   Aquatic   Plants.      1979   Progress    Report.
     Sasketchewan  Research Council.  Saskatoon,  Sasketchewan,
     Canada.

Lawson, G. T. 1985.   Cultivating  Reeds  (Phragmites australis)
     for Root Zone Treatment of Sewage.   Report  totheWater
     Research Centre of the Institute of  Terrestrial Ecology,
     Cumbria, U.K.

                              67

-------
Martel,  C.  J.,  T.  F.  Jenkins,  C.  J.  Diener, and P. L. Butler,
     1982.   Development  of  a Rational  Design  Procedure for
     Overland  Flow-Systems,   CRREL Report 82-2,  U.  S. Army
     CRREL, Hanover,  New Hampshire,  29p.

Mudroch,  A.,  and  J. A.  Capobianco.   1979.   "Effects  of
     Treated Effluent on  a  Natural Marsh."   Journal  of the
     Water  Pollution  Control  Federation.   51;2243.

Nichols, D. S.  1983.   "Capacity  of Natural Wetlands to  Remove
     Nutrients   from  Wastewater."   Journal  Water Pollution
     Control Federation,  55:495.

Otta, J. W., T. G. Searle,  and  S.  V.  Geddes.   1984.    "Land
     Treatment  Enhances  Habitat  of the  Endangered  Mississippi
     Sandhill  Crane."   Proceedings  of  Water  Reuse Symposium
     III,  San Diego,  CA~American Water  Works Association,
     Denver, CO, pp.649-659.

Phillip  J. Clark  Engineers  &  Consultants.  1983.   Marsh
     Pond/Overland Flow  Pilot Plant Project  Report. Goshen,
     NY.

Phillip J.  Clark Engineers  & Consultants.  1981.   Project
     Status Report for  the  Moodna  Basin  Marsh/Pond/Overland
     Flow Pilot  Treatment Plant.  Goshen, NY.

Reddy, K.  R.  and D.  L.  Sutton.  1984.   "Waterhyacinths for
     Water   Quality  Improvement  and  Biomass  Production."
     Journal of  Environmental  Quality,  13;1.

Reed, S.,  Bastian,  R.,   and  Jewell, W. 1980.   "Engineering
     Assessment   of   Aquaculture   Systems   for   Wastewater
     Treatment:   An  Overview."   Aquaculture Systems for
     Wastewater    Treatment;   An  'Engineering    Assessment7
     EPA-430/9-80-007, 1-12.

Reed, S. Environmental Scientist, U. S. Army CRREL, Hanover,
     New Hampshire, personal  communication.

Reed,  S.,   C.  Bastian,  S.   Black,  and  R.  Khettry.  1984.
     "Wetlands   for Wastewater Treatment  in  Cold  Climates."
     Proceedings Water Reuse  Symposium  III,  San  Diego, CA.,
     American Water Works Association,  Denver,  CO.

Reed, S. C.,  1984.   Nitrogen Removal  in Wastewater   Ponds,
     CRREL  Report  84-13.   U. S. Army  CRREL,  Hanover, N.H.
     26p.
                              68

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Richardson, D.  L.  and G.  T.  Daigger.  1983.    "Aquaculture:
     The Hercules  Experience."   Journal of  Environmental
     Engineering Division,  Proceeding  American  Society  of
     Civil  Engineers,  110,  940.

Schwegler, B. R.  and R. H.  Kadlec.  1978.    "Wetlands and
     Wastewater."    Pamphlet,   Wetlands  Ecosystem   Research
     Group, University of Michigan, Ann Arbor.

Shaw, B. H. 1984.   "Municipal  Wastewater Treatment Using an
     Ombrotrophic  Bog."  Unpublished  Paper.    University  of
     Wisconsin, Stevens Point,  WI.

Sloey,  W.   E.,  et  al.  1978.    "Management  of  Freshwater
     Wetlands   for   Nutrient    Assimilation."     Freshwater
     Wetlands. Ecological Processes and Management Potential.
     IT.  E.  Good,  et al. (eds.)   Academic Press,  New York, NY.

Small, M. M.  1978.   "Wetlands Wastewater  Treatment Systems."
     State  of Knowledge  in Land  Treatment of  Wastewater,
     International  Symposium,  U. S. Army  CRREL,  Hanover, NH,
     pp.141-147.

Small, M. M. 1983.   "Marsh-Pond Systems."  Proceedings of the
     Low-Cost   Wastewater   Treatment   Workshop,   Dept.  of
     AgriculturalEngineering,ClemsonUniversity,   Clemson,
     S.C.

Small,  M.   1976.    "Marsh/Pond  Sewage  Treatment   Plants."
     Freshwater  Wetlands   and   Sewage   Effluent  Disposal.
Edited by D.
Richardson.
pp. 197-213.
, L. T
The

ilton, R. H. Kadlec, and C.
University

of

Ann

Arbor ,

. J
MI

Smith, R.  G.  et al.,  1983.    "Performance  of Overland Flow
     Wastewater Treatment Systems  -  Summary Report."  Dept.
     of Civil  Engineering,  University of California,  Davis,
     CA.

Spangler, F. L. , C.  W. Fetter, Jr.,  and  W. E. Sloey. 1977.
     "Phosphorus Accumulation--Discharged  Cycles  in  Marshes."
     Water Resources  Bulletin,  13(6);1191-1201.

Spangler, F. L. , W.  E. Sloey,  and C. W.  Fetter,  Jr. 1976.
     "Experimental   Use  of   Emergent  Vegetation   for  the
     Biological  Treatment   of   Municipal   Wastewater   in
     Wisconsin."  Biological Control  of  Water Pollution,  J.
     Tourbier  and  R.  W.  Pierson,  Jr.  (eds.)  University  of
     Pennsylvania Press,  Philadelphia, PA.,  pp.161-171.
                              69

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Stewart, E. A.,  D.  L.  Haselow, and  N.  M.  Wyse.   1984.   "A
     Practical  Model for  Water Hyacinth  Based  Wastewater
     Management—Design  and  Operation."    Proceedings  Water
     Reuse  Symposium III,  San Diego,  CA., American  Water
     Works Association,  pp.679-702.

Stowell, R.  E.,  R.  Ludwig,  J. Colt, and  G. Tchobanoglous.
     1980.   Toward  the  Rational  Design  of Aquatic Treatment
     Systems.   Dept. of  Civil Engineering, University of
     California,  Davis,  CA, 59p.

Stowell, R. E., et al.  1981.   "The Use of Aquatic  Systems for
     Wastewater Treatment: An  Assessment."   Publication No.
     65,  California  State  Water   Resources  Control  Board
     Sacremento,  CA.

Stowell, R., R. Ludwig,  J. Colt, and G.  Tchobanoglous.  1981.
     "Concepts in Aquatic Treatment  System Design."  Journal
     of Environmental Engineering Div.,  Proc. ASCE. 107:919.

Stowell, R. S. Weber, G.  Tchobaoglous, B.  A.  Wilson,  and K.
     R. Townzen. 1985.    "Mosquito Considerations in  the
     Design  of   Wetland   Systems   for  the   Treatment   of
     Wastewater."    Ecological Considerations  in  Wetlands
     Treatment of  Municipal  Wastewater,  Godfrey,   P. J.,  et
     al. ,  (eds.) , Van Nostrand Reinhold,  New York,  1985,
     pp.38-47.

Sutherland,  J.  C.  1979.     "The  Vermontville,  Michigan,
     Wastewater-Grown  Volunteer  Seepage   Wetlands:   Water
     Quality Engineering  Implications  (abstract)."  Presented
     at the  Conference  on  Freshwaer Wetlands  and Sanitary
     Wastewater Disposal,  Higgins Lake, MI,  July 10-12.

Swett,  D.   1979.    "A  Water  Hyacinth  Advanced  Wastewater
     Treatment System."   Aquaculture  Systems for Wastewater
     Treatment;	Seminar    Proceedings    and    Engineering
     Assessment,   EPA-430/9-80-006,QT   S~.   EPA,   Washington,
     D.C.,  pp.233-255.

Tchobanoglous, G. and G.  L. Gulp. 1980.  "Aquaculture Systems
     for Wastewater  Treatment:   An  Engineering Assessment."
     EPA-430/9-80-007,  U.  S.  EPA, Washington, D.C.

Tuschall, J. R.,  P.  L.  Brezonik, K.  C. Ewel.  1981.  "Tertiary
     Treatment  of  Wastewater  Using  Flow-Through  Wetland
     Systems."   Proceedings of  the National  Conference of
     American  Society  of  Civil  Engineers,  198TIAmerican
     Socity of Civil Engineers, New  York.
                              70

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U.  S.  EPA,  Center  for  Environmental Research  Information.
     1981.   Process  Design  Manual  for  Land  Treatment  of
     Municipal Wast-ewater.  EPA 625/1-81-013.

U.  S.  EPA, Region  IV.  et al.,  1983. Environmental  Impact
     Statement.  Phase  1  Report   Freshwater   Wetlands   for
     Wastewater  Management.    EPA-904/9-83-107,  [~.  S~.  EPA,
     Atlanta, GA.

U.  S.  EPA,  Region  IV.   1985.   Freshwater  Wetlands  for
     Wastewater Management Handbook~.  EPA-404/9-85-135, U.  S.
     EPA, Atlanta,  GA.

U.  S.  EPA,  Office  of  Water  Program   Operations.   1979.
     Aquaculture Systems  for  Wastewater  Treatment;   Seminar
     Proceedings	and	Engineering	Assessment.
     EPA-430/9-80-006, U.  S.  EPA,  Washington, D.C.

U.  S.  EPA,  Office  of  Water  Program   Operations.   1980.
     Aquaculture  Systems  for  Wastewater   Treatment;	An
     Engineering Assessment.   EPA-430/9-80-007,  U.  S.  EPA,
     Washington, D.C.

U. S. EPA, Criteria and Standards  Division.   1982.   Benefits
     and Implementation Potential  of  Wastewater  Aquaculture.
     UI S~. EPA, Washington,  D. C.

U. S. EPA, Region V,  Eastern  Energy and Land Use  Team.  1984.
     The Ecological Impacts  of Wastewater  on  Wetlands;    An
     Annotated   Bibliography/EPA-9 05/3-8 4-00 2.       0".    S.
     Environmental  Protection Agency,  Region V, and U.  S  Fish
     and Wildlife Services,  Eastern Energy and Land Use Team,
     Chicago, IL.

U.  S.  EPA,  Region  V,  Wapora  Inc.  1983.   The  Effects  of
     Wastewater  Treatment  Facilities on  Wetlands   in   the
     Midwest.  EPA-905/3-83-002,  U. S. EPA Region V,  Chicago,
     IL.

Valiela, I., S.  Vince,  and  J. M.   Teal. 1975.   "Assimilation
     of Sewage by Wetlands."   Estuarine Processes, M.  Wiley,
     ed., Academic  Press,  New York, NY.  pp.234-253.

Wang,   H.   K.   1984.     "Sewage   Irrigation  in   China."
     International  Journal for Development Technology,  ^:4.

Weber, S.  A.  and G.  Tchobanoglous. 1982.  "Factors  Affecting
     Nitrogen Removal in  Water Hyacinth  Treatment  Systems."
     1981  National   Environmental Engineering   Conference,
     American Socity of  Civil Engineers,  New York.
                              71

-------
Wile,  I.,  G. Palmateer, and  G.  Miller.   1981.   "Uses of
     Artificial     Wetlands     in    Wastewater    Treatment."
     Proceedings of the Midwest Conference on Wetland Values
     and Management.  St. Paul, MM.  pp. 255-271.

Wile, I., G. Miller, and S. Black,  1985.  "Design and Use of
     Artificial   Wetlands."     Ecological  Considerations  in
     Wetlands Treatment of  Municipal  Wastewaters ,   P .' J .
     Godfrey, et al. (eds.),  Van Nostrand  Reinhold,  New York,
     pp. 26-37.

Williams, R. B., D. J. Reardon, J.  Schefchik. 1985.   "Design
     and Startup  of  770-Acre Wetlands  Project."   Annual
     California  Water Pollution Control  Federation Conference
     and Short  School,  Anaheim, CA. , May 1985.

Williams, T. C.  and J.  C.  Sutherland.  1979.   "Engineering,
     Energy, and  Effectiveness Features of   Michigan  Wetland
     Tertiary Wastewater Treatment  Systems."    Aquaculture
     Systems for  Wastewater  Treatment:  Seminar  Proceedings'
     and    Engineering   Assessment,    EPA    430/9-80-006,
     pp. 141-173.

Wolverton,  B. C.  1979.  "Engineering  Design Data for Small
     Vascular Aquatic  Plant  Wastewater  Treatment  Systems."
     Aquaculture  Systems  for  Wastewater  Treatment;  Seminar
     Proceedings _ and _ Engineering _ Assessment,    EPA
     430/9-80-006, pp. 179-191.

Wolverton,  B. C.  1981.  "Hybrid Wastewater  Treatment System
     Using   Angerobic  Microorganisms  and  Reed  (Phragmites
     Communis)."  Economic  Botany 36:373-380

Wolverton,  B. C. 1985.   The  Role of  Plants and Microorganisms
     in Assuring  a  Future  Supply of Clean  Air  and  Water, A
     Summary of  NSTL  Research.   National  Space Technology
     Laboratories, NSTL,  MS.

Wolverton,  B. C.  1986.   ' Microbial-Plant  Filters  (Artificial
     Marshes) for  Treating  Domestic Sewage  and  Industrial
     Wastewater."    Presented  at  Louisiana Department  of
     Environmental Quality  Seminar,  Baton  Rouge, LA.

Wolverton,   B.  C.  and R.  C. McDonald.    1981.   "Natural
     Processes  for Treatment of Organic Chemical Waste."  The
     Environmental Professional.  3:99
Wolverton,  B. C. and  R.  C. McDonald.  1982.   "The Role  of
     Vascular Aquatic  Plants  in  Wastewater Treatment."   The
     Herbarist.   48:25-29.
                              72

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Wolverton, B.  C. ,  R.  C.  McDonald, and  W. R.  Duffer.  1983.
     "Microorganisms   and  Higher   Plants   for   Wastewater
     Treatment."  Journal of Environmental Quality. 12;236.

Wolverton, B.  C.,  R. C.  McDonald,  and  L. K. Marble.   1984.
     "Removal  of  Benzene and  Its Derivatives  from  Polluted
     Water   Using   the   Reed/Microbial   Filter  Technique."
     Journal   of   the   Mississippi   Academy   of   Sciences.
     29;119-127.

Wolverton,   B.  C.   and  R.   C.  McDonald-McCaleb.   1985.
     "Biotransformation of Priority Pollutants Using Biofilms
     and Vascular Plants."   Submitted  to Mississippi Academy
     of Science Journal.

Yonika, D.  A., et al.  1979.  "Feasibility Study  of  Wetlands
     Disposal   of   Wastewater  Treatment   Plant   Effluent."
     Research  Project  78-04,   Massachusetts  Water  Resource
     Commission, Division of Water Pollution Control.

Zoltek, J. and S.  E. Bayley. 1978.  Removal of Nutrients from
     Treated   Municipal  Wastewater  by  Freshwater  Marshes.
     Centerfor Wetlands,Gainesville,FL.~
                              73

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