United States
             Environmental Protection
             Agency
              Office of
              Research and Development
              Washington, DC 20460
EPA/600/2-91/062
December 1991
oEPA
A Model of Virus
Transport in
Unsaturated Soil

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                                          EPA/600/2-91/062
                                          December  1991
 A MODEL OF VIRUS TRANSPORT IN UNSATURATED SOIL
                       by
                   M.V. Yates
   Department of Soil  & Environmental Sciences
            University of California
               Riverside,  CA 92521

                   S.R. Yates
                    USDA/ARS
            U.S. Salinity Laboratory
              Riverside, CA  92501

                    Y.  Ouyang
   Department of Soil  & Environmental Sciences
            University of California
               Riverside,  CA 92521
      Interagency Agreement No. DW12933820
                 Project Officer

                David M. Walters
     Processes and Systems Research Division
Robert S. Kerr Environmental Research Laboratory
                 Ada, OK  74820
ROBERT S. KERR ENVIRONMENTAL RESEARCH LABORATORY
       OFFICE OF RESEARCH AND DEVELOPMENT
     U. S. ENVIRONMENTAL PROTECTION AGENCY
              ADA, OKLAHOMA  74820

                                    
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                                  DISCLAIMER

The information in this document has been funded wholly or in part by the United
States  Environmental Protection  Agency under  interagency  agreement no.  DW-
12933820 to the United  States Department of Agriculture United States Salinity
Laboratory.   It has  been subjected  to the Agency's peer and administrative
review, and it has been approved for publication as an EPA document.  Mention of
trade  names  or   commercial  products  does  not  constitute  endorsement  or
recommendation for use.
All  research  projects  making  conclusions  or  recommendations    based  on
environmentally related measurements and funded by the Environmental Protection
Agency are required to participate in the Agency Quality Assurance Program.  This
project did not involve environmentally related measurements and did not involve
a Quality Assurance  Project  Plan.
                                       ii

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                                   FOREWORD

      EPA is charged  by  Congress  to  protect the Nation's land,  air,  and water
systems.  Under a mandate of national  environmental laws focused on air and water
quality,  solid waste  management and control  of toxic substances, pesticides,
noise, and radiation,  the agency strives to formulate and implement actions which
lead to a compatible balance between human activities and the ability of natural
systems to support  and nurture life.

      The Robert S.  Kerr Environmental Research Laboratory is the Agency's center
of expertise for investigation of the  soil and subsurface environment.  Personnel
at the  Laboratory are responsible for management of  research  programs  to:  a)
determine the  fate, transport,  and transformation rates of pollutants  in the
soil,  the  unsaturated  zone,  and  the saturated  zones  of  the  subsurface
environment; b)  define the processes to be used in characterizing the soil and
subsurface environment as  a  receptor of pollutants;  c)  develop techniques for
predicting  the  effect  of pollutants  on ground water,  soil,  and  indigenous
organisms; d) define and demonstrate  the applicability and limitations of using
natural processes,  indigenous to  the soil  and subsurface environment,  for the
protection of  this  resource.

      The model  described herein can be used to predict the fate and transport
of  disease-causing viruses   in unsaturated soil.     It  can  be  used to  help
researchers  design experiments  so  that important  transport  parameters  are
measured  accurately.  It can  also be used in conjunction with a saturated flow
model to  help  determine  placement  of waste  sources  relative  to drinking water
wells to  minimize the potential for waterborne viral  disease.
                                                Clinton W. Hall
                                                Director
                                                Robert S. Kerr Environmental
                                                  Research Laboratory
                                      iii

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                                   ABSTRACT

      As a result of the recently-proposed mandatory ground-water disinfection
requirements to  inactivate viruses in potable water supplies,  there  has been
increasing interest  in virus fate  and transport in the  subsurface.   Several
models have been developed to predict  the fate of viruses in ground water, but
few include transport in the unsaturated zone,  and all require a constant virus
inactivation rate.  These are serious limitations in the models, as it has been
well documented that considerable virus removal occurs in  the unsaturated zone,
and  that  the  inactivation  rate  of  viruses  is  dependent on  environmental
conditions.  The purpose of  this research was to develop a predictive model of
virus fate and transport in unsaturated soils that allows the virus inactivation
rate to vary based on changes in soil temperature.  The model was developed based
on the law of mass conservation  of a contaminant in porous  media and couples the
flow  of water, viruses,  and heat through  the soil.  Model  predictions were
compared to measured data  of virus transport in laboratory column studies, and
were within the 95%  confidence limits of the measured concentrations.  The model
should  be  a  useful  tool for anyone wishing to estimate  the number of viruses
entering  ground  water after traveling through the  soil  from a contamination
source.  In addition, model simulations were  performed to identify variables that
have a large effect on the results.  This information can be used to help design
experiments so that important variables are measured accurately.
                                      iv

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                               TABLE OF CONTENTS

                                                                        Page
Disclaimer	ii

Foreword	iii

Abstract	iv

List of Figures	vii

List of Tables	ix

Introduction  	  1
      Transport Processes   	  2
      Factors Affecting Transport Processes  	  3
            Soil  Water Content  	  3
            Soil  Temperature  	  3
            Water Application and Evaporation  	  4
            Soil  Heterogeneity  	  4
Objectives	    	4
Development of Transport Equations  	  4
      Water and Heat Transport  in the Soil	6
      Virus Transport in Soil	10
      Boundary Conditions   	   11
            Boundary Between the Atmosphere and the Soil Profile ....   11
                  Water	   11
                  Heat	14
                  Viruses	18
            Boundary Between the Soil Profile and the Water Table  ...   18
      Solution of Transport Equations   	   20
VIRTUS: a Model of Virus Transport  in Unsaturated Soil 	   26
      Model Applications and Limitations	26
      Verification of VIRTUS  	   26
            Input Parameters	27
            Results	28
      Model Simulations	28
            Simulation 1.  Virus Transport Through Loam Soil With
              Temperature-Dependent Inactivation Rate  	   28
                  Input Parameters	28
                  Results	36
            Simulation 2.  Virus Transport Through Loam Soil With
              Constant Inactivation Rate 	   42
                  Input Parameters	42
                  Results	42
            Simulation 3.  Virus Transport Through a Loam Soil With
              Inactivation Rate Dependent Upon Adsorption State  ....   45
                  Input Parameters	46
                  Results	46
            Simulation 4.  Virus Transport Through an Unsaturated Sand .   46
                  Input Parameters	48
                  Results	48

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                                                                       Page
      Model Testing	48
            Example 1.  Virus Transport in a Saturated Gravelly
              Sand Column	55
                  Input Parameters	55
                  Results	55
            Example 2.  Virus Transport in an Unsaturated Loamy  Sand .  .  55
                  Input Parameters	55
                  Results
Discussion  .
Conclusions
References  .
Appendix I:
Appendix II:
                                                                          55
                                                                          60
                                                                          62
                                                                          63
            SOLVING THE VIRUS TRANSPORT EQUATIONS  	   66
            : DEFINITIONS OF MATHEMATICAL SYMBOLS AND UNITS 	   67
Appendix III: VIRTUS USER MANUAL	71
Appendix IV: LISTING OF INPUT AND OUTPUT FILES 	   93
Appendix V: DISTRIBUTION OF SOFTWARE 	  137
                                      vi

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                             LIST OF FIGURES

Figure                                                               Page
   1     Schematic diagram of a soil profile for which the model
         is developed	   5

   2     Gaussian Pill Box concept for water flow at the
         atmosphere-soil interface   	  12

   3     Gaussian Pill Box concept for heat flow at the
         atmosphere-soil interface   	  15

   4     Gaussian Pill Box concept for virus flow at the
         atmosphere-soil interface   	  19

   5     Comparison of analytical and numerical solutions of
         virus transport equation at 5 hours	29

   6     Comparison of analytical and numerical solutions of
         virus transport equation at 10 hours	30

   7     Virus concentration as a function of soil depth using
         a temperature-dependent inactivation rate in an Indio
         loam soil, simulation 1	37

   8     Virus concentration as a function of time using
         a temperature-dependent inactivation rate in an Indio
         loam soil, simulation 1	38

   9     Soil-water content as a function of time in an Indio
         loam soil, simulation 1	39

  10     Surface evaporation or condensation as a function of
         time in an Indio loam soil, simulation 1	40

  11     Soil temperature as a function of time in an Indio
         loam soil, simulation 1	41

  12a    Differences in predicted virus concentrations using
         a temperature—dependent (Cet)  vs.  constant  (C10)
         inactivation  rate in an Indio loam soil, simulation 2a  .  .  .  43

  12b    Differences in predicted virus concentrations using
         a temperature-dependent (Cct)  vs.  constant  (C2s)
         inactivation  rate in an Indio loam soil, simulation 2b  .  .  .  44

  13     Effect of assuming no inactivation of adsorbed viruses
         (Cnu«) vs. a non-zero inactivation rate of adsorbed
         viruses (C^,) on model predictions for an Indio loam
         soil, simulation 3	47
                                   vii

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                            LIST OF FIGURES

Figure                                                               Page

  14     Virus concentration as a function of soil depth using
         a temperature-dependent inactivation rate in a Rehovot
         sand soil, simulation 4	52

  15     Virus concentration as a function of time using
         a temperature-dependent inactivation rate in a Rehovot
         sand soil, simulation 4	53

  16     Soil-water content as a function of time in a Rehovot
         sand soil, simulation 4	54

  17     Comparison of model predictions to experimental data,
         Example 1	57

  18     Comparison of model predictions to experimental data,
         Example 2	59
                                  viii

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                            LIST OF TABLES

Table                                                               Page

  1.    Equations for water, heat, and virus transport through
        the soil	21

  2.    Boundary conditions at: (1) the interface between the

        atmosphere and  the soil surface; and (2) the water table.  .  22

  3.    Parameters for the virus properties in simulation 1  ....  31

  4.    General input parameters for simulations 1, 2, and 3 ....  32

  5.    Values of the soil parameters used for the Indio loam
        soil that remain constant throughout the simulation  ....  33

  6.    Values of the soil parameters used for the Rehovot sand
        that remain constant throughout the simulation 	  49

  7.    Parameters for the virus transport properties in the
        simulation	51


  8.    Data used for model testing, example 1	56


  9.    Data used for model testing, example 2	58
                                  ix

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                                 INTRODUCTION

      The significance of viruses as agents of ground-waterborne disease in the
United  States  has been  well documented (Craun  1986,  1990).   The  increasing
interest in preventing ground-water contamination by viruses and other disease-
causing  microorganisms has  led to  new  U.S.  Environmental Protection  Agency
regulations regarding ground-water disinfection (U.S.EPA,  1991), the development
of wellhead  protection zones, and  stricter standards  for  the  microbiological
quality  of municipal sludge  (U.S.EPA, 1989) and treated effluent (California
Department of Health Services, 1990) that is applied to land.   For many of the
new regulations, a predictive model  of virus (or bacterial) transport would be
helpful in the implementation process.   For example, such a model could be used
to determine where  septic tanks could be placed or where  land application of
sludge  or effluent  could be practiced  relative to  drinking  water wells  to
minimize negative impacts on the ground-water quality.  Another application of
microbial transport  models  is  related  to  the ground-water disinfection  rule
(U.S.EPA, 1991).  Water utilities wishing to avoid ground-water disinfection may
use a pathogen transport model to  demonstrate that adequate removal of viruses
in the  source water occurs during  transport to the wellhead.
      Several models of microbial transport have  been  developed during the past
15 to 20 years (Grosser, 1984; Harvey and Garabedian, 1991; Matthess  et al.,
1988; Park et al., 1990;  Teutsch et al.,  1991; Tim and Mostaghimi, 1991;  Vilker
and Burge, 1980; Yates and Yates,  1989).  The models  range from the very simple,
requiring few  input  parameters,  to the  very  complex,  requiring numerous input
parameters.  For many of the more complex models,  the data required for input are
not available  except for very limited environmental  conditions.   They  may be
useful for research purposes, but would  be impractical for widespread use.  The
potential applications of these models also range considerably, from being useful
only for screening purposes  on a regional scale,  to predicting virus behavior at
one specific location.
      One limitation of  almost  all of  these  models  is  that  they have  been
developed to describe virus  transport in saturated soils (i.e., ground water).
However, it has been demonstrated many times that  the potential for virus removal
is greater in the unsaturated zone than  in the ground water (Keswick and Gerba,
1980; Lance and Gerba,  1984;  Powelson et al.,  1990).  Neglecting the unsaturated

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 zone  In any model of virus transport could lead to inaccurately high predictions
 of  virus concentrations  at the  site of  interest.    This  omission would  be
 especially significant  in areas with  thick unsaturated zones, such as those in
 many western states. The one transport model  (Tim and Mostaghimi,  1991) that has
 reportedly been  developed for predicting virus transport in variably saturated
 media  is  not specific for viruses, but  can  be used for any contaminant.   In
 addition, It has not been tested using data  of virus transport in unsaturated
 soil.
      Another,  and more  important,  limitation of  published models  of virus
 transport is that none  of them has been validated using actual  data of virus
 transport in unsaturated soils.   Most  models  are developed based on theory, and
 are fitted to data obtained  from one or two experiments. Rarely are they tested
 by applying the model to data collected under a variety of conditions and then
 determining how well the model predicts what has been observed in the laboratory
 or field without any fitting or calibration of the model.

TRANSPORT PROCESSES

      The transport of viruses  through a  porous medium  such as soil is affected
primarily by the following mechanisms and processes:
      1.     Advection.   The advection of viruses  or any other contaminants in
            water  is due  to the  average velocity of  water  as  it flows.   It
            results in the entire mass of contaminant streaming from a zone of
            higher potential to one of lower potential.
      2.     Hydrodynamic dispersion.  Hydrodynamic dispersion is the spreading
            of a contaminant caused  by mechanical  dispersion  and  molecular
            diffusion.   Dispersion is the nonsteady irreversible mixing of two
            miscible fluids  displacing  one  another.    Diffusion is a  random
            motion of molecules caused by thermal kinetics.
      3.     Adsorption  (and  desorption).   The  adsorption  of viruses to  soil
            particles is caused by  a  combination  of  electrostatic and van  der
            Waals forces and hydrophobic interactions between  the virus and soil
            particles.   Adsorption reduces the concentration  of viruses  in  the
            soil water.  Desorption occurs due to changes in the ionic strength
            of the soil  water.

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      4.     Filtration.  The physical filtration of viruses during the transport
             process  occurs primarily by straining and sedimentation.  Straining
             occurs when  the particles in suspension in the porous matrix cannot
             pass  through a smaller pore, and  thus  their transport is halted.
             For very small particles such as viruses, filtration is generally
             neglected (Corapcioglu and Haridas, 1986). However,  if the viruses
             are adsorbed onto a solid particle entrained in  the water or are
             present  as  aggregates,   filtration  can  be  an important  removal
             process.  Sedimentation of viruses  in the soil pores occurs when the
             density  of virus  particles is higher than that of water.
      5.     Inactivation.  Virus inactivation is  the loss of infectivity toward
             host  cells,   and  therefore  ability   to  causes  disease.    The
             inactivation of viruses is caused by  a variety of adverse chemical,
             biological,  and physical  processes.

FACTORS AFFECTING TRANSPORT PROCESSES
      The transport of viruses through soil is controlled  by climatic conditions
such as the  rate  of rainfall (or water application)  and evaporation and by soil
properties   such  as  soil  water  content,  soil   temperature,  adsorption  and
desorption,  filtration, soil pH, and salt concentration (Yates  and Yates, 1988).
The  properties  of  the  specific  virus   of  interest  are also  important  in
determining  its behavior in the subsurface.   Some  of the  most important factors
that affect  the transport  of viruses through soil research include soil water
content, soil  temperature,  the rate  of water application and  evaporation,  and
soil heterogeneity.
Soil Water Content
      The  amount  of  water in  the  soil   (soil water content) influences  the
movement of  viruses  through soil.  Lance and Gerba  (1984) found that saturated
flow through loamy sand resulted in 7% recovery of poliovirus  at a depth of 10
cm, but unsaturated flow resulted in only 0.5% recovery.   Powelson et al. (1990)
found that MS-2 virus was removed to a much greater extent  under unsaturated flow
conditions as compared to that during saturated flow conditions.
Soil Temperature
      Soil temperature affects the  length of  time that viruses remain infective
in the  environment.   At  lower temperature,  virus persistence  is  prolonged
                                       3

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 compared with  that  at higher  temperature  (Yates  and Yates,  1988).   Soil
 temperature  also  affects  the  transport  of  soil  water,   thereby indirectly
 affecting the transport of viruses through soils.
 Water Application and Evaporation
       Addition of water (e.g., by rainfall or irrigation)  to the soil acts to
 move viruses through the  soil profile.   It can also act to desorb previously
 adsorbed viruses,  thus  allowing  them to be transported deeper through  the soil.
 The evaporation of water out of  the soil surface causes the  changes in the soil
 water content and  soil  temperature, thereby affecting the transport and fate of
 viruses.
 Soil Heterogeneity
       Soil  is a heterogeneous system whose properties change with soil depth.
 At  different soil layers, soil  properties such as  soil  porosity, hydraulic
 conductivity, and  thermal conductivity, are different.  Therefore,  the transport
 of  viruses  through soil, which is subjected to the soil properties, will change
 with soil depth.

                                   OBJECTIVES

      The purpose  of this  research was to develop a model  that can be used to
 predict virus movement  from a  contamination source through unsaturated soil to
 the  ground  water.   Several model simulations were  performed to -determine the
 effects of  different input variables on model predictions. The model was tested
 by  comparing model predictions to results  of  laboratory  studies.
      The specific objectives  of this  project were:
      1.    To develop a mathematical model to describe the transport of viruses
            in unsaturated soil  that includes factors  specific to viruses, and
      2.    To test model predictions with experimental data  of virus transport
            in soil.

                      DEVELOPMENT OF TRANSPORT EQUATIONS

      Transport equations were derived  to describe the simultaneous transport of
water, viruses,  and heat for a soil profile shown schematically in Figure 1.  The
 soil profile, which may contain  soil with  nonhomogeneous properties, is bound

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SOLAR RADIATION


!      1     1
     RAINFALL
                              1     1
               I*    C'
                 BOUNDARY LAYER
       (2.: )    T ( r. t  )  C,  i z. t )
                 TYPICAL SOIL LAYER
                   WATER TABLE
                                        (ATMOSPHERE)
                                         (SOIL SLAB)
                                                          £7
                                                              -  Z
                                                          z = z
                                                                N
Figure 1.    Schematic diagram of a soil profile for which the model

            is developed

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  below by a water table  and above by  the atmosphere.
   The  assumptions used in the  development of transport equations pertaining to
 the  atmosphere  are:
       1.     Diurnal  changes in  air  temperature  and relative  humidity can be
             characterized by a Fourier series;
       2.     Diurnal  changes  in solar  intensity  can  be  characterized  by a
             Gaussian normal distribution function;
       3.     The initial distributions of water and temperature in the atmosphere
             are known;
       A.     The rate and duration of water application is prescribed.
       The assumptions used  in the development of  transport equations pertaining
 to the soil profile  are:
       1.     The soil can be characterized by known parameters such  as:  the soil
             porosity;  the  specific heats of solid, water, and  air; the thermal
             conductivities  of  solid, water,  and  air;  the  latent   heat  of
             vaporization;   the  water   potential  function;   the  hydraulic
             conductivity  function; and the densities of solid, water,  air, and
             water vapor;
       2.     The atmosphere  and soil  surface are  coupled for the water, virus,
             and heat fields by heat  and  mass  transport rules operating in the
             boundary layer  at  the atmosphere-soil interface; and
       3.     The initial distributions  of water content,  temperature, and viruses
             in  the soil profile  are prescribed.

WATER AND HEAT  TRANSPORT  IN THE  SOIL

      Water and heat transport equations  derived  by Ouyang (1990) are given as:
                                      = -v [Pre V> + Pw (e-6)

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for water transport,  and:

               -A [  (i-e) Cfolid pgolid T +  (e-6)  c^r f>Mir T + 6 c

                       - -v • [ (i-e) if,.  + 6 Htl + (e-6) H,v ]                    (2)
for heat transport,  where t is the time (hr). pw is the density of water  (g
cnf3),  6 is the volumetric water content  (cm3 cm"3),  ffi (T) is the  density  of
water vapor at saturation  at  temperature T (g vapor cm"3 air),  h is the relative
humidity (dimensionless),  c is the soil porosity (cm3 soil voids  cm"3 soil)
^i and Vv are the velocity vectors of water in the liquid and  vapor  phases (cm
hr"1) ,  respectively,  pm is the density of water vapor  (g vapor cm"3 air). cBoLid
is the  specific heat of soil  particles (cal soil"1 particle "C"1)  , ptoud *-s tne
density of soil particles (g cm~3 solids),  T  is  the  temperature (°C) ,  cair and cw
are specific  heats  of air and water  (cal  g"1 "C"1) ,  respectively,  pair  is  the
density of air (g cm~3air) ,  H*,, is the  vector  of heat conduction  through the soil
particles (cal cm"2 hr"1) . flsl  is the vector of heat conduction and convection in
the liquid phase  (cal cm"2 hr"1) , and tfsv is the vector of heat conduction in the
vapor phase and the  latent heat flux (cal cm"2 hr"1) .
      The velocity vectors, VL and Vv,  in  equation  (1)  are defined as:

                       Vj = - V"6 - $r/vr + *• (* -  |f)  -                    (3)
and
with
                                                                             (6)

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                                                                             (7)
                                     tare [<-=

                                                .                            (9)

where Dei Is the second rank tensor describing the diffusion  coefficient of
water in the liquid  phase  (cm2  hr"1) .  DT1 is  the  second tensor describing the
thermal diffusivity  in  the  liquid phase (cn^hr"1) ,  K is the second rank  tensor
describing the water conductivity (cm hr"1) , z~*is the  unit vector normal to plane
z — 0 with positive orientation vertically downward, z  is the  soil depth (cm).
V1 is the water  potential  (cm),  D^ is the second rank tensor  describing the
diffusion coefficient of water  in the vapor  phase  (cm2  hr"1) ,  Djy is  the second
rank tensor describing  the  thermal diffusivity in the vapor phase (cm2 hr"1) ,
Uj is the second rank tensor describing the  diffusion coefficient of water
affected by the water potential (cm2  hr"1) , Datm is the  water vapor molecular
diffusion coefficient in air  (cm2  hr"1) , atort is the tortuosity  factor of the
soil, g is the  gravitational constant (cm hr"1), R is the gas constant (cnf2 hr"2
       ++                                                           sat
°C~1),  E is the second  rank identity  tensor (dimensionless),  and pm is the
density of water vapor  at  saturation  (g vapor cm"3 air).
      The vectors flss, "kgl,  and  flgv in equation (2) are  defined as:
**
                                 ,* m ~  , olid *r '                             (10)
                            Htl - - A.r vr+ C, prvj r

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and
                          **.y - - lDttm«toZt*Prv - X^r vT ,                      (12)



where A.olid is the thermal conductivity of the solids  (cal  cm"1 hr"1  "C"1) ,  AM is

the thermal  conductivity of water  (cal  cm"1 hr"1 "(T1), £ i-s  tne  latent heat of

evaporation  (cal g"1), and Aair  is the thermal conductivity of the air  (cal cm"1

hr'1 "C"1).


     The  variables p^f, p^,  h,  and ^ in equations  (1) and (12). which are
functions of temperature, are given as (Weast,  1986):
                    p"' = 0.004928 + 0. 0002581 T + 0.0000183T2                (13)



for 0 <  T  < 22.5°C,  and




                     pJt' = 0.00493 + 0.000258T + 0.0000396T2                 (14)



for 1 >  22.5°C,




                                                                            (15)
and
                                                                             (17)
                                = 598.88 - 0.547T .

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VIRUS TRANSPORT  IN  SOIL
      According  to the law of conservation of mass, the virus transport equation
in differential  form  can be  expressed as:

                         _|j[PJ, c,. ec, j .-£I8B4|  j

                               -  I », c, . tt t.c. ] - Ofc, .                   <1S)
where t is the time (hr) .  pb  is the bulk density of soil (g cm"3 soil) ,  C, is  the
concentration of viruses adsorbed onto the soil particles  (mass g"1 solid), 8 is
the volumetric soil water content (cm3 cm"3),  Cx  is the  concentration of  viruses
in soil water (mass cm"3 water),  z is  the  soil depth  (cm) ,  D is the  coefficient
of hydrodynamic dispersion of viruses  (cm2 hr"1) , V is the flow  velocity of water
(cm hr"1) ,  /*! and  nt are the  inactivation coefficients  of viruses  in liquid  and
solid phases (hr"1) , respectively, and f is the filtration coefficient of  viruses
(hr"1).
    The adsorption of viruses onto soil particles, Cg, in  equation  (18) can be
expressed by using the Freundlich adsorption isotherm (Grosser,  1984;  Corapcioglu
and Haridas, 1986):
                                  C. = Kd Of ,                               (19)

where Cs is the concentration of viruses  adsorbed onto  the  soil particles  (mass
g"1  solid) ,  Kj is  the  Freundlich  constant (cm3 water  g"1  solid) ,  n  is  the
exponential constant (dimensionless), and C± is  the concentration of viruses in
the soil water (mass cm"3 water).  For many systems,  the empirical constant n is
not significantly different from unity and equation (19) reduces to a linear form
(Vilker and Burge,  1980; Yates  and Yates, 1988):
                                                                           (20)
                                      10

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BOUNDARY CONDITIONS
      Equations  (1),  (2), and  (18) define the coupled transport of water, heat,
and virus, respectively.  Boundary conditions must be defined to be able to solve
these equations.
Boundary Between the  Atmosphere and the Soil Profile
Water
      By using the  "Gaussian Pill Box" concept (Figure 2) ,  the conservation of
mass law requires that:

                                                 3 - -z) - 0 ,               (21)
where qw is a vector describing the total water flux at the soil surface  (g water
cm"2 hr"1) ,  n is  the unit vector outward going normal to the simple closed
surface,  z is the unit vector normal to plane z - 0 with positive orientation
vertically downward, q*rain is a vector describing rainwater flux into the soil  (g
water cm""2 hr"1) ,  and q^vap is a vector describing evaporation flux or condensation
flux of water out of,  or into, the soil (g water cm"2 hr"1) .
      The total  water  flux vector q^, at the soil surface (z - 0) can be  defined
as :

                       *» l..o - [8oPw?z +  PvX]  |,.0 ,                   (22)
                           -*
the rainwater flux vector qrain is  written as:
                                                                            (23)

and  the  evaporation or condensation flux vector q.vap is given as:
                                       11

-------
                 evao
                               'ram
                                      n = - z
ATMOSPHERE
    SOIL
                       t
                         iw
                                      n = + z
Figure  2.   Gaussian Pill Box concept  for water flow at the
           atmosphere-soil interface
                              12

-------
                                          (T \     T -T
                                          t ^t' Q / •*cj*«
                                 * P-'^.x)] ,                        (24)

where B0 is the water content at the  atmosphere-soil  interface  (cm3 cm"3) ,  pv is
the density of water  (g cm"3) ,  ^ is  the velocity vector of liquid water (cm hr~
*) .  c0 is the soil porosity at the atmosphere-soil  interface (cm3 soil voids cm"3
soil) , pm is  the density of water vapor (g cm"3) . V^ is the velocity vector of
water vapor (cm hr"1) , i^in is the vector describing the rainfall rate (cm hr"1) ,
 *
D.tni is the the boundary layer wind-speed-dependent coefficient  of dispersion of
water vapor (cm2 hr"1) ,  p"fc (Ta) is the density of water vapor at saturation at
temperature T.  (g vapor cm"3) ,  T» is  the temperature  in  the atmosphere  (°C), 66
is the relative humidity in the atmosphere (dimensionless),  T0 is the temperature
at the atmosphere-soil interface (°C) ,  6z is the thickness of  the boundary  layer
at the atmosphere-soil  interface (cm), and h is the  relative humidity at the
atmosphere-soil  interface (dimensionless).
      Substituting  equations (22), (23)  and (24)  into equation (21) yields:
                                                     n
                                     .           .                          (25)
      Recall  that  equations  (3)  and (4)  are written as:
                                               (z-
                         Vv = -
                                       13

-------
 and n - z,  substituting tfL and Vv  into  equation (25)  leads to:
                                   r.0
                               Krv ' "'  *z  '  '                             (26)

 which is the coupling upper boundary condition for water transport.
 Heat
       Analogous to the movement of water, again using the Gaussian  Pill  Box
 concept (Figure 3),  conservation of heat requires that:
                        + qh»(n - z)  = 0 ,                                    (27)

where qheatin is a vector describing heat flux  into  the  soil  surface via
rainwater  (cal cm"2 hr"1),  qhteVp is a  vector  describing  heat  flux out of
the soil surface  due to evaporation or heat flux into the soil surface due  to
condensation  (cal cm"2 hr"1),  qhtiwr is a vector describing heat flux into  the
soil surface by short wave radiation  (cal cm"1 hr"1) , qht§»i *s  a vector describing
                                       14

-------
                      heann
                I bAMU^
                 IIIArr
                htev
n = -
n   =  +  2
q       q
^ htssi     httwrs
                                                htNvra
                                                     ATMOSPHERE
                                                             z = 0
                                                         SOIL
                                Hs
 Figure 3.   Gaussian Pill Box concept for heat  flow at the


             atmosphere-soil interface
                                15

-------
 heat flux through the soil surface via  sensible heat (cal cm"2 hr"1) ,
 a vector describing heat flux  into the  soil surface via long wave  radiation
 (cal cm'2 hr"1) ,  qttiwr* *s a vector describing heat flux out of the  soil
 surface via long wave radiation  (cal  cm"2 hr"1) ,  and qj,  is  a vector describing
 total heat flux into, or out of,  the  soil  surface (cal cm"2 hr"1) .
       The vectors in equation  (27) are  defined by (Lindstrom and Piver,  1985):
                                                                             (28)
                                                                             (29)
                                                   (e0-60)
                                           T -T
                                      1*  /  o  • \
                                    - *«*r(  &z  )  ,                           (31)
                                     [0.605 + 0.048] .                    (32)
                                                         - 60)] ,              (33)
                                            +  «0 -  0,V ,                    (34)
with
                                                                            (35)
                                       16

-------
                                                                           (36)
Definitions of variables  in equations  (28)  through  (36)  are  in Appendix II.
      Substituting equations (28) through (36) into equation (27) with subsequent
combination of variables  leads  to:
                          [(1 -
                                                  dT,
[(1 -ej (1 -
                                           + 60(1  -aMt.J
                                   (e0-60)(l -
                                [0.605 * 0.048/e7n]
                                                  (37)
                                       17

-------
which is  the coupling upper boundary conditions for heat transport.
Viruses
       Analogous  to the transport of water and heat, and again using the Gaussian
Pill  Box  concept (Figure  4),  the conservation of virus mass requires that:


                          qv»(n*  z) + Gziijtf* -f)  • 0 ,                     (38)
where qv is a vector describing virus flux into the soil surface (mass cm'2 hr'1)
and  qrain  is  a vector describing virus  flux  through the soil surface by
infiltration (mass  cm'2 hr'1) .
     The vectors  in  equation (38)  are defined as:
                                                                           (39)

and

                                Grain = C«n $r«in •                            (40)

where Cwin  is  the  concentration of virus in water entering the soil (mass
cm'1  water) .
      Substitution of equations  (39) and (40) into equation (38) yields:
which is the coupling upper boundary  condition for virus transport.
Boundary Between the Soil Profile  and the  Water Table
      The boundary conditions at the interface between the soil profile and the
fixed elevation water table  for water  and heat  transport  are assumed  to  be
constant.  These are:
                                                                           (42)
                                 Q(ZW,  t) - e,,
and
                                       18

-------
  ATMOSPHERE
      SOIL
                       'ran
                       t
n =  - z
                                                     Z= 0
                                    n- 4  2
Figure 4.    Gaussian Pill Box concept for virus flow at the
            atmosphere-soil interface
                                19

-------
                                     , t) - Tg ,                              (43)
where z, is the soil depth at the interface between the  soil  slab  and the water
table  (cm) ,   6t is the saturated soil water  content  (cm3  cm"3) . and Tg is  the
temperature at  water  table  (°C) .
      The boundary condition for the virus field at the interface between  the
soil slab and the water table is not fixed.  This boundary  condition does  not
occur  except  for  short periods of  time,  insufficient  for  the viruses to be
transported from  the  top or from a buried source position to  the water table.
The more realistic boundary condition is obtained by applying the  Gaussian Pill
Box concept to  virus  transport  at the interface between the  soil  and the water
table and is  given as (Lindstrom et  al.,  1990):
                r (*   f\ - '  o *   *'    »"   » /'     +    i
                C2(zv,  t) = ( —*- t- 	—	)/(T*T~ + TT'  '
                            AZ,        AZHf        *ZN*   *Z.

where C].(zw, t) is  the  concentration of virus  at the  interface  between the  soil
profile and the water table at time t (mass cm*3 water) , Cg is the  concentration
of  virus  in the ground  water (mass cm*3 water).  zw is  the  soil  depth at  the
interface between  the  soil and the water  table (cm).  and AzNz and  Az*  are  the
depth intervals above and below the interface between  the soil profile  and the
water table, respectively  (cm).
      The  water,  heat,   and  virus transport  equations  and  their boundary
conditions, discussed above,  are  summarized in  Tables  1  and 2.
SOLUTION OF TRANSPORT EQUATIONS
      Since the solution of the virus transport equation (18) requires a point-
wise knowledge of water and heat distributions in space and time,  the water and
heat transport equations  (1)  and  (2) must  be  solved prior to solving the virus
transport equation.  The three transport equations were solved  using the finite
difference  method.   Solution of  the water and heat  transport equations were
discussed and given in Ouyang (1990).  Solution of the  virus transport equation
is discussed below.

                                       20

-------
Table  1.   Equations for water, heat,  and virus transport through the soil.
Water
                                  (e-6) ] - -* • tpr e v, + Pw
Heat
                                    T * (e-6) c.lr p.ir T * 6
                                          6 Htl +  (e-6) H.v
                           c.
                                        21

-------
Table  2.   Boundary  conditions  at:  (1)  the  interface between  the  atmosphere  and




           the  soil  surface;  and  (2)  the water  table.
         Interface  Between  the Atmosphere and the Soil Surface
Water
                                              <-Jf )
                            - -fj) U
                              Tj +  
-------
Table 2.  Boundary conditions at:  (1) the interface between the atmosphere  and

          the  soil surface;  and (2) the water table (continued).
                                   (e0-00)(l -«^r)]g«T


                                            eo + e-ir(e0 - 80)]
                                 |[0.605 * 0.
Virus
                 Interface Between the Soil Profile and the Water Table

Water
                                  e(*w,  t) - e.
Heat
                                  T(zw, t)  - Tg
Virus
                                       23

-------
      Before  approximating equation (18), define:
                                                ,                            (45)
and
                           A « 6jij Ca + pb M.C.
Putting equations  (45)  and (46)  into equation (18) yields:
                             [P» C. + Be, ] -•£[«) -A.                     (47)
      Following Varga's (1962) method of approximation, integration of both sides
of  equation (47) over  the rectangular subregion of space  and time  [  (Zi-iiz,
zi+1/2) x (tj,,  t^) ]  yields:
                              "
                                                 tp^c.+ecj tdz
                        -    /      /    [A]dzdt.                             (48)

      Applying the  finite difference formulations  in Appendix I,  substituting
equations (45) and  (46) into  equation (48), and defining C, - Kj C1(  obtains:
                                               i.i/2
                                       24

-------
                                                 Qf J^c/;1} .                (49)
      Multiplying both sides of equation (49) by 2/(&zi + Azi+1/2) and

rearranging  terms into common coefficients yields:
                              rial?:?/,  +        /,
                                                    J
                                                    me,-/
                                     j-.                                     (50)
which  is  a useful form for setting up the computer  program.
                                        25

-------
            VIRTUS:  A MODEL OF VIRUS TRANSPORT IN UNSATURATED SOIL

      The  mathematical  model  developed herein  was  entitled VIRTUS   (VJRus
Jransport in Jlnsaturated £oil) , and programmed in FORTRAN for use on IBM and IBM-
compatible PC's.  A  document describing  the use of the program is contained in
Appendix III.   Sample input and output data that can be used to test the model
are  listed  in Appendix  IV.   In this  section, the  mathematical model  and
corresponding  computer  program,  VIRTUS,  will be demonstrated  in  a variety of
situations.  The potential  applications  of this model and its limitations will
also be discussed.
MODEL APPLICATIONS AND LIMITATIONS
      Some of  the features  of this model include its ability to simulate:
      1.    unsteady flow in variably-saturated media
      2.    transport in layered soils
      3.    variable virus  inactivation  rate (e.g., function of temperature)
      A.    different virus  inactivation  rates  for  adsorbed versus  freely
            suspended virus particles.
      5.    the flow of heat through soil  (which  affects water  flow,  virus
            inactivation rate, etc.)
As the model was programmed, the viruses  are  applied  to  the soil surface.  This
occurs when treated sewage effluent is used for irrigation purposes, where ground
water is recharged with effluent,  and when sewage effluent is discharged to dry
stream beds  (such as occurs in  the  southwest).   If viruses are applied to the
soil from a buried source, such as a septic tank, the model may still be applied;
however, the boundary conditions in the  program must be adjusted.  VIRTUS also
assumes that there is a water table at  the bottom of  the  soil profile, if there
Is not, the appropriate boundary conditions must be introduced.
VERIFICATION OF VIRTUS
      The numerical solution of the virus transport equations was verified using
an analytical  solution to  the  equations.    The  virus  transport  equation and
initial and boundary conditions used were:

                             IP* C. * 6Cj ] * -^ ( 6D-£ ]
                                                                          (51)
                              - [ 6ti; Cj + pb \i,c, ] -
                                      26

-------
                                  Cj(z, 0) « 0  ,                              (52)

                                 C,(0. t)  - Cc ,                              (53)
and
                                  Cj(«, t) - o .                              (54)

      The  analytical solution of equations (51), (52), (53), and (54) is:
                   (z  t)  m  C°
                  jU, t)  - _
                                   6V-Z
                                                          '                  ^   ^

    Equations  (51).  (52),  and (53) were  also solved using VIRTUS by a numerical
method  (with C1(z1,t) - 0 at  the lower boundary z - zx).
Input Parameters
      Input parameters  for the simulation were as follows:
      1.    Hydrodynamic dispersion coefficient:  Dx - 4 cm2 hr"1;
      2.    Saturated soil water content: 0, - 0.3  cm3  cm"3;
      3.    Freundlich  constant:  K,,  - -0.02 ml g"1 soil;
      4.    Inactivation coefficient: px - 0.001  hr'1;
      5.    Average  linear flow velocity of water:  vi - 0.1  cm hr"1;
      6.    Initial  virus concentration: C0 -  105 PFU ml"1;
                                       27

-------
       7.    Bulk density: pb - 1.65  g cm"3; and
       8.    Soil depth: z - 100 cm.
 Results
       Comparisons of predictions made using the analytical  solution versus  the
 numerical solution of the virus transport equation are in Figures 5 and 6.  These
 figures show the  relative  virus concentration as  a function of soil depth at
 times of 5 and 10 hours.  Good agreement was obtained between the analytical  and
 numerical solutions as shown in the  Figures.
 MODEL SIMULATIONS
       The capabilities  of VIRTUS to simulate the simultaneous transport of water,
 viruses, and heat  are  demonstrated  using data  for two different  soil types.
 Several simulations were performed to  show the  effects  different variables on
 model predictions.
 Simulation 1.   Virus  Transport  Through Loam Soil With  Temperature -Dependent
 Inactivation Rate.
       This simulation calculated virus concentration profiles  during transport
 through an unsaturated loam soil. The rate of virus inactivation changed as a
 function of soil  temperature  throughout the  course of  the transport process .
 Viruses adsorbed to soil  particles were assumed to have an inactivation  rate of
 zero.
 Input Parameters
       The specific values of the variables related to virus fate and transport
 are shown  in Table 3.    The values  were  chosen from published data of  virus
 transport obtained from experiments conducted under conditions similar to  those
 used in the simulation.
       General input values for the simulation are shown in Table 4.  The physical
 properties of the Indio loam soil are listed in Table  5.  Parameters that varied
 as  a function of  time  included air  temperature, relative humidity  of  the  air
 above the soil  surface, and solar radiation.   These variables  were  represented
 by  the functions described below.  These functions are only approximations of  the
'daily changes measured in nature.
       The daily course of air temperature can  be  characterized by a  Fourier
 series (Ouyang,  1990):
                                                                .          (56)

                                       28

-------
    (J
    Q_
    0)
   Q
    O
   00
         0
         20
         40
         60
         80
        100
0.2
     C/Co

   0.4      0.6
                                             0.8
   Numerical  Solution

—» Analytical  Solution
Figure 5.   Comparison of analytical and numerical solutions of

           virus  transport equation at 5 hours
                             29

-------
                                C/Co
                      0.2
   0.4
0.6
0.8
       £
       o
       Q_
       CD
       O
       O
       00
            20
            40
            60
            80
           100
   Numerical  Solution

— > Analytical  Solution
Figure 6.   Comparison of analytical and numerical solutions of

           virus transport equation at 10 hours
                            30

-------
Table 3.  Parameters  for  the virus properties  in  simulation  1.
Parameters
Values/Units
                                 References
Dispersion
  coefficient
Distribution
  coefficient

Filtration
  coefficient

Inactivation*
  coefficient
  in liquid phase

Inactivation
  coefficient
  in solid phase
- OtortDlo + Qdiip I VI
- 0.66 x 0.000324 +
  10 I VI   cm2 hr'1

- 0.27 ml g'1  soil
f - 0.0 cm'1
     [(-0.181  + 0.0214  T)
     LnlO]/24 hr'1
/*. - 0
                              Grosser, 1984,
                              Bales et al., 1989
                              Lindstrom et  al., 1990

                              Powelson et al. , 1990
                             Yates and Yates,  1988
* T is soil temperature  (°C).
                                       31

-------
Table  4.   General  input parameters for simulations 1, 2, and 3.
 Parameters
                                Values
                         Units
 Simulation  time

 Simulation  time
   step

 Simulation  soil depth

 Simulation  depth
   step

 Surface  infiltration
   rate

 Surface  infiltration
   duration*

 Initial  soil water
   content

 Initial  soil
   temperature

 Initial  soil virus
   concentration

Virus concentration
   in the surface
   infiltration water

 Initial virus
   concentration in
   ground water

Virus type

Soil type
120

0.025


100

1.0


0.1
0.25


8.7


0.0


105



0.0



MS-2 bacteriophage

Indio loam soil
hrs

hr


cm

cm


cm hr"1


hrs
cm3 cm"3
PFU ml"1
PFU ml
      -i
PFU ml
      -i
*Surface infiltration started at 0th hour and  ended  at  6th hour in the morning
of the first day.
                                      32

-------
Table 5.  Values  of the  soil  parameters used for the Indio loam soil that
          remain  constant  throughout  the  simulations 1, 2, and 3.
Symbol
a.ir
Q«oil
°tort.
«~t.r
"<
fit
c.ir
cel«y
c««nd
c«ilt
c..,..
£
£
Meaning
Albedo of air
Albedo of soil
Tortuosity factor
Albedo of water
Coefficient in
equation (59)
Coefficient in
equation (59)
Specific heat
of air
Specific heat
of clay
Specific heat
of sand
Specific heat
of silt
Specific heat
of water
Total porosity
Emissivity of air
Value /Units
0.05
0.09
0.66
0.07
1241.39
cm
0.7079
0.24 cal g"1
oc-i
0.175 cal g'1
oc-i
0.175 cal g-1
oc-i
0.175 cal g-1
oc-i
1.0 cal g-1
oc-i
0.55
0.9
Reference
Weast, 1986
Ghildyal and Tripathi, 1987
Hillel, 1982
Weast, 1986
Ouyang, 1990
Ouyang, 1990
Weast, 1986
Ghildyal and Tripathi,
1987
Ghildyal and Tripathi,
1987
Ghildyal and Tripathi,
1987
Weast, 1986
McCoy, et al . , 1984
Weast, 1986
          above  the soil

«.oll    Emissivity of       0.5
          coil surface
        Emissivity of       0.95
         water

        Saturated           0.61
         conductivity  in      cm hr"1
         equation  (60)
pmil     Density of air      0.0011 g cm
                                       -3
Ghildyal and Tripathi,
  1987

Weast,  1986
                                              Ouyang, 1990
                                               Weast,  1986
                                       33

-------
Table  5.  Values  of the  soil parameters used  for  the  Indio  loam  soil  that
          remain  constant  throughout  the  simulations  1,  2,  and 3.
Symbol    Meaning
                              Value/Units
                                             Reference
f>b
Pcifj
Bulk Density of     1.2 g cm
 soil
                                    '3
P.ilt
7C
 •olid
 air
 Density of clay

 Density of sand

 Density of silt

Density of water

Coefficient  in
  equation (60)

Thermal
  conductivity
  of solids

Thermal
  conductivity
  of water

Thermal
  conductivity
  of air

Residual soil
  water content        cm

Saturated  soil
  water content        cm
                    2.64 g cm'3

                    2.66 g cm'3

                    2.65 g cm'3

                    1.00 g cm'3

                    6.5
                    18.9  cal
                     cm -1 hr'1
                            5.14 cal
                             cm'1 hr'1
                    0.2214  cal
                     cm'1 hr'1
                            0.029 cm3
                               '3
                            0.55 cm3
                               '3
                                             McCoy, et al.,  1984


                                             Ghildyal and Tripathi,  1987

                                             Ghildyal and Tripathi,  1987

                                             Ghildyal and Tripathi,  1987

                                              Veast, 1986

                                             Ouyang,  1990
                                              Ghildyal  and  Tripathi,
                                               1987
                                      Ghildyal  and Tripathi,
                                       1987
                                             Ghildyal and Tripathi,
                                                1987
                                     Ouyang, 1990


                                     Ouyang, 1990
                                      34

-------
where T,lr(t) is the air temperature (°C) at time t, T is the mean air temperature
(°C) ,  N  is the number  of the Fourier  frequency,  A^^Cn)  and Bt-np(n) are  the
temperature coefficients, u^ is the n** temperature Fourier frequency, and t is
time (hr).
      The  relative humidity  in the atmosphere can be characterized by a Fourier
series (Ouyang, 1990) :
              RH^z(t) - RH + Y^.1 [Azb(n) COB (w^t) +5^(23) sin (to^t)] ,          (57)


where RHmlt (t) is the  relative humidity (dimensionless) at time t,~RH  is  the
mean relative humidity (dimensionless),  N is the number of the Fourier frequency,
Arh(n)  and Brh(n) are the relative humidity coefficients, u^  is the nth relative
humidity  Fourier  frequency,  and t is time (hr) .
      The Gaussian normal  distribution function of the  form  (Ouyang,  1990):
                         QSR(t)  * ^expt-0.5      *   ] .                     (58)
                                              **i

was used to describe rate of solar radiation as  a function of time of day, where
QSR(t) is the intensity  of solar radiation (cal cm'2 hr"1)  at time  t; E:, E2,  and
£3 are coefficients of the equation,  and  t is time.
      The  power  law  function  proposed by van Genuchten  (1980)  was used  to
describe the soil water  potential as a function of water  content:
                                                                           (59)
 where  ^ is  the water potential  (cm),  ae is  a coefficient characterizing  a
specific  soil   (cm),  0e  *s  a coefficient  characterizing  a  specific  soil
(dimensionless) , 6n is the saturated volumetric soil water content (cm3 cm"3), and
6X is the residual volumetric soil water content (cm3 cm"3).   Values of constant
coefficients  in  equation (59)  for  the Indio loam soil are  in Table 5.
                                       35

-------
      The  Kozeny function
                                      , e - e.
proposed  by Mualem  (1976)  was used  to  describe hydraulic  conductivity as a
function  of soil water content.   K is the hydraulic conductivity (cm hr"1),  K,
is the hydraulic conductivity at saturation (cm hr"1),  6 is the volumetric soil
water content (cm3 cm"3), 6X  is the residual volumetric soil water  content  at air
dry conditions (cm3 cm"3), 0. is the saturated volumetric soil water content  (cm3
cm"3),  and 7e is a coefficient characteristic  for a specific soil.   Values of
constant  coefficients  in equation (60) for the Indio loam  soil are in Table 5.
Results
      The virus concentration profiles predicted during the simulation are shown
in Figures  7 and 8.   The concentration  of viruses in the top few  cm of soil
changed rapidly  from an initial value of  zero  to more than 5 x 10* PFU/ml  during
the 6  hours of  infiltration  (Figure  7).   Figure 8 shows  the change  in virus
concentration  with time at two  soil depths.   It  can be  seen that  as  the
concentration of viruses begins to decrease at the  5-cm depth after 48 h (as a
result of advection, adsorption, and  inactivation). the concentration at 10 cm
begins to increase as  a  result of transport through the soil.
      Changes  in soil water content  during 48 hours  simulation are  shown in
Figure 9.  This  simulation  started with a water application period from  0 to 6
hours  at  a  rate of 0.1 cm hr"1.    The  application  rate  was lower  than  the
infiltration capacity  of the soil, so that the infiltration profiles at 6 hours
did not show the constant water content of a transmission zone  followed by a
rapid decrease in water content in the wetting zone or wetting front.  Following
the 6 hours of water application was a period  of  change in the water content of
the soil profile, due to evaporation  (Figure 10) at the soil surface and further
penetration of the water into the soil due to  a water  potential gradient  at the
wetting front.
      Daily cycles of soil temperature at several depths are shown in Figure 11.
This figure  shows that  the temperature  at the soil  surface  was close  to  the
temperature of the applied water  (4 °C). This water,  which completely wetted the
soil surface,  decreased the temperature  of  the  soil surface  layer  to  the
temperature of water at about 6 hours.  As soon as water application stopped at
                                      36

-------
  E
  (J
  CL
  
-------

-------
     E
     U
     CL
     (D

    Q
     O
    Ul
         Soil  Water  Content   (cm3/cm~3 )


           0.24      0.26      0.28      0.3      0.32
         20
         40
         60
         80
         100
i X D
1 ^ 1
« x D
I \ I 	
T x ^"^"^
9 o'x D
T/ 1 /

-a a 0 Ox Ox DX 1 / IX K K 1 t t I o-^"^" ^ ff.^^^"^^ ^ — """^ *— o t = 0 hrs e>— e> t = 6 hrs D— a t = 24 hrs x— x t = 48 hrs Figure 9. Soil-water content as a function of time in an Indio loan soil, simulation 1 39


-------
           -0.02
      Si
      \
       E
       E
 C
,2

 03

 6
 CL
 ro

L^J

 CD
 U
  if}   O
      o
      _g

       03
       en
       c
       CD
           -0.011-
          0.01
                0
                         12
36
48
                             Time   (hrs)
Figure  10.  Surface  evaporation or condensation as a function of

           time in  an Indio loam soil, simulation 1
                             40

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                        12
24
36
48
                            Time  (h)
Figure 11.  Soil temperature as a function of time in an Indio
           loam soil,  simulation 1

-------
 6 hours,  radiation started to  warm the  soil  profile.   The  changes in  soil
 temperature during  the period  from  6  to 48 hours  showed the  characteristic
 temperature cycle, with a decreasing temperature during the night  followed  by
 warming during the day.
 Simulation 2.  Virus Transport Through Loam Soil  With Constant Inactivation Rate.
       Most models  of contaminant transport consider the movement of water and the
 transport of the contaminant in their development, and assume  that  the  thermal
 conditions in the soil remain constant.  In reality, under field conditions, this
 is not generally the case.  Temperature  fluctuations in soil can be considerable
 throughout the  course  of  a  24-hour period,  especially near the soil surface.
 Because the effects of temperature on virus inactivation rates in the  environment
 can  be  quite  significant,  it   seems logical  to use  a model of  contaminant
 transport that also models heat flow.
       This simulation demonstrates  the  effects of holding the  inactivation rate
 of the viruses constant at a) 0.033 Iog10 per day, which  would  be expected at a
 soil temperature of 10 C;  and b) 0.354 Iog10 per day,  which would  be  expected  at
 a soil temperature of 25 C.
 Input Parameters
       The  input parameters used for  this  simulation were  the same  as for
 Simulation 1 and are  shown in Tables 3,  4 and 5.  The only exception is that the
 virus inactivation rate was 0.033 for Simulation 2a and 0.354 for  Simulation 2b,
 rather than calculated as a function of temperature as shown in  Table 3.
 Results
       The effects  of allowing the virus inactivation rate to vary as a function
 of soil temperature as compared  to holding it constant are  graphically shown  in
 Figures 12a and 12b.   In  the case  where the virus  inactivation rate was  held
 constant at 0.033 Iog10 day'1 (10 C),  the model  predicted higher  concentrations
 of viruses than  would be predicted  if the inactivation rate was allowed  to vary
 as a  function of  temperature   (Figure   12a).   The opposite  predictions  were
 obtained in the  case  of a  constant  inactivation rate of 0.354  Iog10 day"1 (25  C)
'as shown in Figure 12b.  Considering the inactivation  rate  to  be a  constant  at
 25 C resulted  in an underprediction in the concentration of viruses  as compared
 with  the temperature-dependent  inactivation  rate.
       The reasons  for these predictions become apparent upon observation of the
 predicted change in soil temperature that occurs as applied water is  infiltrated
                                      42

-------
                    -  C10  (PFU/ml)
       -12000   -9000    -6000    -3000
       0
                         t =  24  h
                         t =  72  h
                         t =  120 h
Figure 12a.  Differences in predicted virus concentrations using
           a temperature-dependent (Cet) vs. constant (C10)
           inactivation rate  in an Indio loam soil,  simulation 2a
                            43

-------
             CCT    -  C25  (PFU/ml)
                 2000    4000     6000     8000
       30
                          t  = 24  h
                     x—x t  = 72  h
                     o-a t  = 120  h
Figure 12b.  Differences in predicted virus concentrations using
           a temperature-dependent (Cct) vs. constant (C25)
           inactivation rate  in an Indio loam soil,  simulation 2b
                            44

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through the soil column (Figure 11).  At the soil surface, over a 24-hour period,
the soil temperature  (which started at 8.7 C) decreased to 3 C at  6 h during the
addition of cold water and increased to 35 C at 12 h due to the effects of solar
radiation.   Similar  patterns  would be  expected  at the  5-  and  10-cm depths,
although the magnitude of the variation would not be  as large.  In  Simulation 2a,
the virus inactivation rate was held constant at a value  that would be expected
for constant 10 C soil conditions.  The fact that the soil temperature rose above
10 C for more than 12  hours in a 24-hour period resulted in a prediction of virus
inactivation  at relatively  high rates  (compared to the rate at a  constant
temperature of 10 C) for that period. Overall, maintaining the inactivation rate
at a constant value had the effect of increasing the predicted concentration of
viruses that were  transported  through  the soil column by more  than 4 orders of
magnitude (Figure  12a).
      In Simulation 2b, the soil temperature was considered to be  constant at 25
C; consequently the virus inactivation was maintained at  a relatively high rate
throughout the transport process.  In actuality,  the soil temperature was at or
above 25 C for a relatively short period of time (less than 6 hours), so viruses
were inactivated at or above that high  rate for only six hours in the simulation
where the rate was temperature  dependent.   In this  case  (Figure 12b),  assuming
a constant inactivation rate would lead to a prediction that thousands of viruses
fewer  than  the  actual  number  (assuming that  the  variable  inactivation  rate
simulation predicts the actual number) would be  transported  through the column.
      The  sensitivity of  model  predictions  to  changes in  the temperature-
dependent inactivation  rate was  determined by  changing  the inactivation  rate
while keeping all  other  variables constant.  This sensitivity analysis showed
that changing the value of the  inactivation rate by 50% resulted in a 33% change
in the predicted  concentration of viruses being transported through  the soil.
A high sensitivity of model predictions to the virus inactivation rate has  also
been observed  by  Tim and  Mostaghimi  (1991) and  Park,  et al. (1990).  These
results demonstrate the  need to  accurately monitor virus inactivation and/or
temperature during experiments  of virus  transport in the subsurface.
Simulation  3.   Virus Transport  Through a Loam  Soil With  Inactivation  Rate
Pependent Upon Adsorption State.
      There have been reports in the literature of differences in measured rates
of virus inactivation for viruses that are adsorbed to soil particles as compared
                                      45

-------
to viruses that are freely suspended In the liquid medium (Hurst et al.,  1980;
Sobsey  et al., 1980;  Vaughn and  Landry,  1983).   Therefore,  this model  was
developed to allow the user to input different values for inactivation rates for
viruses in these two states.  When a value for the inactivation rate of adsorbed
viruses is specified,  the model calculates the number of viruses adsorbed at a
given  time  based  on the adsorption  coefficient specified  by  the user,  and
determines the number  inactivated accordingly.
Input Parameters
      It is difficult to obtain a quantitative value  for the relative difference
In inactivation rates for adsorbed as compared to freely suspended viruses.  For
the purposes  of illustration, this simulation used an inactivation  rate  for
adsorbed viruses equal to one-half that of free viruses.  Inactivation rates for
viruses in the adsorbed  and free state were allowed to change as a function of
the soil temperature.  All other input values are as shown in Tables 3,  4,  and
5.
Results
      The model  predictions made  in  Simulation  3  were  compared to  those of
Simulation  1,  in which  the  inactivation rate  for  adsorbed viruses  was zero
(Figure 13).   As one  would  expect,  the concentration of viruses transported
through the soil column is larger when the solid-phase inactivation rate is zero
than when it is one-half the liquid-phase rate.  The difference increases with
time, as  shown in Figure 13.   In  a system in which  the  inactivation rate of
adsorbed viruses is equal to that of free viruses, the differences would be even
greater.
      This example demonstrates the importance of knowing  the inactivation rate
for viruses in the adsorbed as well as in the liquid  phase. If the inactivation
rate for adsorbed viruses is actually lower than that of  suspended viruses, it
would be important to  incorporate that information in a model so that accurate
predictions can be made  of virus concentration profiles.   If the model assumes
the same inactivation rate for all  viruses,  it would predict  that fewer viruses
are being transported  than the actual number.
Simulation 4.  Virus Transport Through an Unsaturated Sand
      This simulation predicts  virus concentration profiles during transport
through an unsaturated Rehovot sand. This simulation was included to demonstrate
the large differences  in the transport properties of different soil types.
                                      46

-------
            c      -  c
              nus         wus
         (PFU/ml)
  o
  CL
  CD
 Q
      10
         i
         i
 ~   20*
 CO
         11
      30
               1000   2000   3000  4000   5000
—» t =  24 h
x-x t =  72 h
o-o t =  120 h
Figure 13.  Effect of assuming no inactivation of adsorbed viruses
           (Cnu») vs. a non-zero inactivation rate of adsorbed
           viruses (C^,,) on model predictions for an Indio loam
           soil, simulation 3
                            47

-------
Input Parameters
      The general  input values used for this simulation are shown in Table 3,
with the exception of a value of 0.1 cm3 cm"3 for the initial  soil water content.
The  soil physical  properties are shown  in Table 6,  and the virus  fate and
transport properties are listed in Table 7.
Results
      This simulation  illustrates the effects of soil properties on transport.
The Rehovot sand has a much higher hydraulic conductivity (see Table 6) than the
Indio loam (Table 5), thus water and contaminants can move through this soil more
rapidly.   As shown in Figures 14 and  15,  the viruses were transported more
rapidly and in higher concentrations  in  this soil as compared with the loam soil
of the previous examples.  After 6 hours,  the viruses  in the  loam soil had been
transported only 11 cm (Figure 7), as compared with more than 35 cm in the sandy
soil (Figure 14).  The differences between the two  columns become more apparent
at longer times: after 5 days approximately  30 viruses ml"1 had been transported
15 cm in the loam soil; whereas more than 102 viruses  ml"1 were being recovered
in the sand column  effluent  after the same amount of time.
      Changes in soil water content during 120 hours of simulation are shown in
Figure 16. This figure contrasts sharply with that for the loam soil (Figure 9).
      Another reason for the  relatively higher concentrations of viruses being
transported through this soil, in addition to the higher hydraulic conductivity,
is related  to the  adsorption coefficient.   For this  sand,  based  on reported
values for virus adsorption  to  other sandy soils, an adsorption coefficient of
zero was chosen.  Thus, the  rate at which the viruses were transported through
the  soil  was not decreased  as  a  result of adsorption  to  the soil particles,
unlike the case for the loam soil.
MODEL TESTING
      The model was tested for its ability to predict virus movement as measured
in  laboratory  column  studies.   Two  data  sets  that contained  sufficient
information about the soil  properties for the model were obtained. In each case,
the  model was run  using input values  measured or reported by the respective
investigator.  No attempt was made to fit the model predictions to the measured
results.  Model predictions were then  compared with  the  virus concentrations
measured as a function of  soil  depth and time in the laboratory.
                                      48

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Table 6.  Values of the soil parameters used for the Rehovot sand that
          remain constant throughout the simulation.
Symbol
«^
«.0il
atort
«..t.r
ae

fie

C.ir
-,
c»«nd
c«ilt
—
€
«.ir
Meaning
Albedo of air
Albedo of soil
Tortuosity factor
Albedo of water
Coefficient in
equation (59)
Coefficient in
equation (59)
Specific heat
of air
Specific heat
of clay
Specific heat
of sand
Specific heat
of silt
Specific heat
of water
Total porosity
Emissivity of air
Value /Units
0.05
0.35
0.66
0.07
8.8654
cm
1.5024

0.24 cal g-1
oc-i
0.175 cal g-1
oc-i
0.175 cal g-1
oc-i
0.175 cal g"1
oc-i
1.0 cal g-1
oc-l
0.4
0.9
Reference
Weast, 1986
Ghildyal and Tripathi, 1987
Hillel, 1982
Weast, 1986




Weast, 1986
Ghildyal and Tripathi,
1987
Ghildyal and Tripathi,
1987
Ghildyal and Tripathi,
1987
Weast, 1986
Ungs, et al. , 1985
Weast, 1986
4 soil
above  the soil

Emissivity of
soil surface

Emissivity  of
water

Saturated
conductivity in
equation (60)

Density of  air
                            0.3
                            0.95
                            52.8914
                            en hr"1
Ghildyal and Tripathi,
1987

Weast, 1986
                            0.0011 g cm
                                       -3
Weast, 1986
                                      49

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Table 6.  Values of the soil parameters used for the Rehovot  sand that
          remain constant throughout the simulation  (continued).
Symbol    Meaning
   Value/Units
                                                     Reference
lb      Bulk Density of
        soil

Peitj    Density of clay

/>««nd    Density of sand

P»nt    Density of silt

£w«t«r   Density of water

-yc      Coefficient in
        equation  (60)

*«oiid   Thermal
        conductivity
        of solids
        Thermal
        conductivity
        of water

        Thermal
        conductivity
        of air

        Residual soil
        water content

        Saturated soil
        water content
                            1.595 g cnf3


                            2.64 g cm'3

                            2.66 g cm'3

                            2.65 g cm'3

                            1.00 g cm"3

                            3.3421
                            18.9 cal
                            cm -1 hr'1
 5.14 cal
 cm'1 hr'1
 0.2214  cal
cnf1 hr"1
 0.008  cm3
 cm"3

 0.4 cm3
 cm'3
                  Ungs,  et  al.,  1985


                  Ghildyal  and Tripathi,  1987

                  Ghildyal  and Tripathi,  1987

                  Ghildyal  and Tripathi,  1987

                  Veast, 1986
                   Ghildyal and Tripathi,
                   1987
                                              Ghildyal and Tripathi,
                                              1987
                                              Ghildyal and Tripathi,
                                              1987
                                              Ungs,  et al.,  1985
                                               (adapted)

                                              Ungs,  et al.,  1985
                                      50

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Table 7.  Parameters for the virus  transport properties  In  the  simulation.
Parameters
 Values/Units
     References
Dispersion
  coefficient
Distribution
  coefficient

Filtration
  coefficient

Inactivation*
  coefficient
  in liquid phase

Inactivation
  coefficient
  in solid phase

Virus type
ni - <*tortDlo + «di.P I vl
   - 0.66 x 0.000324 +
     10 I Vl   cm2 hr'1

Ke - 0.0 ml g'1 soil
 f - 0.0 cnf1
 Mi - [(-0.181  +  0.0214  T)
      lnlO]/24 hr'1
    - 0.0
 MS-2  bacteriophage
Grosser, 1984,
Bales et al., 1989
Lindstrom et al., 1990

Powelson et al., 1990
Yates  and Yates,  1988
* T is soil temperature  (°C).
                                       51

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  E
  o
  d
  CD
  O

 10
         Virus  Concentration  (PFU/ml)
        10C
101
10s
10-
io4
Figure 14.  Virus concentration as a function of soil depth using


          a temperature-dependent inactivation rate in a Rehovot


          sand soil, simulation 4
                           52

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     30000
                                                         120
                            Time   (hrs)
Figure 15.  Virus  concentration as a function of time using
           a temperature-dependent inactivation rate in a Rehovot
           sand soil, simulation 4
                             53

-------
               Water  Content  (cm3/cm3)
       E
       (J
       Q_
       CD

      Q
       O
      00
o.c
0




20






40



60



80


100
)6 0.08 0.1 0.1
A \ \
• q x
\ \ \
• 0 X
\ \ \
• a x
\ Vo V
\ \ \
• D x
\ A \
• q x
\ \ \
• a x <
\ \ \
* q x
\ " \
* q x <
\ \ \
*\°\
• q *
\ \ \
* q x
/
E>
t>
/
1 O
/
O
/
O
/
t>
/
t>
/
O
/
> &
/
1 &
/
»
/
x>
\A \ \l
« t = 0 hrs V\ U
— t - 6 hrs \ I',
- x-x t = 24 hrs \t I
o-o t = 72 hrs \ 3J
p
1
^
1
»
»
•
VV 1 I
"— • t = 120 hrs \J
\
Figure 16.  Soil-water content as a function of time in a Rehovot


          sand soil, simulation 4

-------
Example 1.  Virus Transport in a Saturated Gravelly Sand Column.
      The  data  used  for  this  example were  obtained  from  virus  transport
experiments using saturated soil columns conducted by Grondin at the University
of Arizona, Tucson  (1987).
Input Parameters
      The values used as input to VIRTUS are shown in Table 8.  All of the values
were determined experimentally by Grondin or calculated by him during fitting of
his data to a model.
Results
      When VIRTUS' predictions were compared to the results obtained by Grondin
(1987) using a saturated soil column, the model predictions were within the 95%
confidence limits of the measured virus  concentrations (Figure 17).
Example 2.  Virus Transport in an Unsaturated  Loamy Sand.
Input Parameters
      The data used as input for this example were obtained from virus transport
experiments  using  unsaturated  soil columns  conducted by  Powelson  at  the
University of Arizona, Tucson and reported  in Powelson et al. (1990).  The input
values  are  listed  in Table  9.    All  values  were  either measured  by  the
investigator or reported by him after model fitting.
Results
      The virus concentration profiles predicted by VIRTUS are compared with the
measured data of Powelson  in  Figure  18.  The model predictions were very close
to  the measured virus concentration profiles,  in all  cases  within  the  95%
confidence limits of the measured virus  concentrations.
                                       55

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Table 8.  Data used for model testing, example 1.  (from Grondin,  1987)
Property
Soil type
Soil bulk density
Hydrodynamic dispersion
Soil water content
Average water velocity
Soil column length
Soil adsorption coefficient
Virus type
Virus inactivation rate
Filtration coefficient
Input virus concentration
Simulation time
Input Value
gravelly sand
1.65 g cm'3
78 cm2 h'1
0.26 cm3 cm"3
48.3 cm h'1
100 cm
-0.054 ml g'1  soil
MS2 coliphage
0.082 loglo day'1
0 cm'1
6.3 x 103 pfu ml'1
48 min
                                      56

-------
      Depth (cm)
tn
                20
                40
                60   -
                80
               100
               120
                          I   95% C.L.
                              Measured
                         -0-  Predicted
                    0
       2           3
Concentration (log pfu/ml)
                             Figure 17.  Comparison of model predictions to experimental data,
                                       Example 1

-------
Table 9.  Data used for model testing, example 2. (from Powelson et al.  (1990))
Property
Soil type
Soil bulk density
Hydrodynamic dispersion
Soil water content
Average water velocity
Soil column length
Soil adsorption coefficient
Virus type
Virus inactivation rate
Filtration coefficient
Input virus concentration
Simulation time
Input Value
loamy fine sand
1.54 g cnf3
92.24 cm2 h'1
variable with depth
1.54 cm h'1
100 cm
0 ml g"1  soil
MS2 coliphage
2.00 Iog10 day'1
0 cm'1
105 pfu ml'1
4 days
                                      58

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Depth (cm)
          20
          40
          60
          80
         100
         120
                                                          I
95% C.L.
Measured
Predicted
                                   4                    5                   (
                                Concentration (log pfu/ml)
                       Figure 18.  Comparison of model predictions to experimental data,
                                 Example 2

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                                  DISCUSSION

      The ultimate measure of a model's usefulness as a predictive tool is its
ability  to  accurately predict field  observations  of virus transport  under  a
variety  of environmental  conditions.    However,  most models  that have  been
developed to  predict microbial transport have not been tested  using  field or
laboratory data.  There are a few exceptions to this (e.g. ,  Teutsch et al. , 1991;
Harvey and Garabedian, 1991).  However,  both of these models were developed for
use by the investigators in order to simulate their own data.  In the case of the
colloid  filtration model of Harvey  and Garabedian,  extensive  fitting  of the
required input parameters was performed by calibrating different solutions of the
transport equation to  the observed bacterial breakthrough curves.  Thus, while
these models  may be  able to  simulate the investigator's  data reasonably well,
they may not  be able to predict  the  results of other investigator's transport
experiments.  If a model is to be used for purposes  other than research, such as
for community planning or  for  making regulatory  decisions,  it  must  be able to
predict microbial transport using data obtained by anyone under  a wide range of
environmental conditions.
      In this research, a model to describe virus transport was  developed based
on the factors known to affect virus  fate  in the  subsurface.   A survey of the
literature was conducted to  locate data sets in which the investigators made
measurements  of  not  only virus  properties,  but  also   soil  and  hydraulic
properties.  Two data sets were located  and used to test VIRTUS.  No fitting or
calibration of the model was performed; the data and measurements as reported by
the  respective  investigators  were used  as model input.   Model predictions
compared favorably to measured experimental data.  However, only one example of
a comparison to one laboratory transport  study in unsaturated soil using a single
soil type and a single virus type was performed.
      In addition, the temperature-dependent inactivation rate capabilities of
the model could not be  tested.  This is due  to the fact that the experiments were
conducted under constant temperature conditions in the laboratory, thus  the virus
inactivation rate remained constant (theoretically) throughout the course of the
experiment.   In  order to test  the  model's capacity  to calculate new virus
inactivation  rates as  a  function of the changing soil temperature, data from a
laboratory study  in  which the  temperature  is allowed to change  (and is closely
                                      60

-------
monitored) or from a field study in which the temperature is monitored will be
required.  This will  allow an assessment of the model's capability to accurately
calculate heat  flow through the soil, which affects water flow (and thus virus
transport) as well as  the rate of virus  inactivation during  transport.   More
testing of the  nodel is required before using  it  for any purposes other than
research.
                                      61

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                                  CONCLUSIONS

      This research project has  resulted  in the  development of a mathematical
model that can be used to predict virus  (or bacterial) transport in unsaturated
soils.  The model  allows  the  user to  specify  the virus  inactivation rate as a
function of soil temperature.   It will also  allow the user to specify different
inactivation rates for adsorbed versus freely suspended virus particles, if that
information is available.
      A sensitivity analysis of the model indicated that the inactivation rate
of the virus has a large effect on model predictions. The adsorption coefficient
and dispersivity also affect model predictions, although to a smaller extent.
      Model predictions compared  favorably  to  two data  sets against which the
model was tested.  However, there is a  lack of data available  for extensive model
testing.  No complete  data sets from field transport experiments were found that
could be used to test VIRTUS.    Before  the  model can  be used for any purposes
other than research, it should be extensively tested using actual field data.
      In its present  condition,  the model requires the user  to  input several
pieces of information related  to  climatic  conditions.  It also requires a large
amount of information characterizing the physical properties of the soil, as do
most models of contaminant transport.  Before VIRTUS could be used for purposes
other than research,  a user interface, extensive  help facilities, and a library
of soil and virus properties would have to be added to the model.
                                      62

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                                  REFERENCES

Bales,  R. ,  C.  Gerba, G.  H.  Grondin,  and S. Jensen.   1989.   Bacteriophage
      transport  in sandy soil and  fractured  tuff.   Appl.  Environ.  Microbiol.
      11:2061-2067.

Bitton, G., and  C. P. Gerba.   1984.  Groundwater pollution microbiology:   The
      emerging  issue.  pp. 1-7.  In:  Groundwater  pollution  microbiology.   G.
      Bitton  and C.  P. Gerba, eds.  John Wiley & Sons, Inc.,  New York.

Brady,  N.  C.    1984.  The  nature and  properties  of soils.   Macmillan
      Publishing Company, New York.

California Department of Health  Services.  1990.  Initial statement of reasons
      for  proposed  changes  in  the  regulations  of  the  Department of  Health
      Services   pertaining  to  the  use of  reclaimed water other  than  for
      groundwater recharge and  pertaining to use  of household gray water  at
      residences and text  of proposed  regulations.   Sacramento.

Corapcioglu,  M.  Y. , and A.  Haridas.  1986.  Transport and  fate of microorganisms
      in porous  media: A theoretical investigation.  J. Hydrol.  72:149-169.

Craun,  G. F.  1986.  Vaterbome Diseases in the United  States.  CRC Press,  Boca
      Raton,  Florida.

Craun,  G. F.  1990.   Review of the  causes  of waterborne disease  outbreaks.  In
      G.F.  Craun (ed.),  Methods  for the  investigation and  prevention  of
      waterborne  disease  outbreaks,  USEPA,   Washington  D.C. publication  no.
      EPA/600/1-90/005a.

Frind,  M. 0., W. H.  Duynisveld,  0.  Strebel, and J. Boettcher.  1990.   Modeling
      of multicomponent  transport with microbial  transformation in groundwater:
      The Fuhrberg case.  Water  Resour. Res.  26:1707-1719.

Ghildyal, B. P., and R. P. Tripathi. 1987.   Soil physics.  John Wiley & Sons, New
      York.

Grondin, G. H.   1987.  Transport  of MS-2 and f2 bacteriophage through saturated
      Tanque  Verde Wash soil.    Master's thesis.   Department of  Hydrology and
      Water   Resources  Administration,  The   University  of  Arizona,   Tucson,
      Arizona.

Grosser, P. W.   1984.  A one-dimensional mathematical model of virus transport.
      Proc. Second International Conference  on Ground-Water  Quality Research,
      Tulsa,  Oklahoma.

Harvey. R. W., and S. P.  Garabedian.  1991.  Use of  colloid filtration theory in
      modeling  movement of  bacteria  through a  contaminated sandy  aquifer.
      Environ. Sci.  Technol.  21:178-185.

Hillel, D.    1982.   Introduction to  soil  physics.  Academic Press,  Orlando,
      Florida.

                                       63

-------
Keswick, B. H., and C. P. Gerba.  1980.  Viruses in groundwater.  Environ. Sci.
      Technol.  14:1290-1297.

Lance, J. C., and  C.  P.  Gerba.  1984.  Virus movement in soil during saturated
      and unsaturated flow.  Appl. Environ. Microbiol. 47:335-337.

Lindstrom, F. T. ,  L.  Boersma, and S. Yingjajaval.   1990.  CTSPAC: Mathematical
      model for coupled transport  of water, solutes,  and heat in the soil-plant-
      atmosphere continuum.  Vol.  1. Mathematical theory and transport concepts.
      Tech. Rept.   Dept. of Soil  Science,  Oregon  State University,  Corvallis,
      Oregon.

Lindstrom, F. T., and W. T.  Piver.  1985.  A mathematical model of the transport
      and the fate of toxic chemicals in a simple  aquifer.  Tech. Rept. No. 52,
      Oregon State University, Department of Mathematics,  Corvallis, Oregon.

Matthess, G., A. Pekdeger,  and J. Schroeter.  1988.   Persistence and transport
      of  bacteria  and  viruses in  groundwater-A  conceptual  evaluation.   J.
      Contain. Hydrol. 2:171-188.

McCoy,  E.  L. ,  L.  Boersma,  M.  L. Ungs and  S.  Akratanakul.    1984.   Toward
      understanding soil water uptake by plant roots.  Soil Sci. 137:69-77.

Moore, W. J. 1983.  Basic physical  chemistry.  Prentice-Hall,  Inc., Englewood
      Cliffs,  New Jersey.

Mualem, Y.   1976.   A new model for predicting the  hydraulic  conductivity  of
      unsaturated  porous media.  Water Resour. Res.   12:513-522.

Ouyang, Y.   1990.   Dynamic mathematical model of  oxygen and  carbon dioxide
      exchange  between  soil and atmosphere.   Ph.D.  Thesis.   Oregon  State
      University,  Corvallis, Oregon.

Park, N.,  T. N.  Blandford, M. Y. Corapcioglu, and P. S. Huyakorn.   1990.  VIRALT:
      A  modular  semi-analytical  and  numerical  model for  simulating  viral
      transport  in ground  water.   Report to  U.S.  Environmental  Protection
      Agency, Office  of Drinking Water, Washington,  D.C.

Powelson, D. K., J.  R.  Simpson, and C. P.  Gerba.    1990.   Virus transport and
      survival  in  saturated and  unsaturated  flow  through  soil columns.   J.
      Environ. Cnial. 1£:396-401.

Teutsch, G.f K.  Herbold-Paschke,  D.  Tougianidou,  T.  Hahn,  and  K.  Botzenhart.
      1991.   Transport of microorganisms in  the  underground -  processes,
      experiments, and  simulation models.   Wat.  Sci. Tech.  24:309-314.

Tim, U.S., and S. Mostaghimi.  1991.  Model for predicting virus movement through
      soils.  Ground Water  22:251-259.
                                      64

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Ungs, M.  J.,  L.  Boersma, and S. Akratanakul.   1985.  OR-Nature: The numerical
      analysis  of  transport  of water  and solutes  through soil  and  plants.
      Volume  IV.  Examples.   Special  report 754.   Agricultural  Experiment
      Station,   Oregon State University, Corvallis, Oregon.

U.S.  Environmental Protection Agency.   1989.  Standards  for  the  disposal  of
      sewage  sludge;  proposed  rule.   Fed. Regist.   54:5746-5902.

U.S.  Environmental Protection  Agency.   1991.   Possible  requirements  of  the
      ground-water disinfection rule. (6/20/91).    Office  of  Ground Water  and
      Drinking Water,  Washington,  D.C.

van Genuchten, M. Th.  1980. A close  form equation for predicting the hydraulic
      conductivity of unsaturated  soils.  Soil Sci. Soc.  Am. J. 44:892-898.

Varga, R. S.  1962.  Matrix iteration analysis.  Prentice-Hall, Englewood Cliffs,
      New Jersey.

Vilker, V. L. , and W. D. Burge.  1980.  Adsorption mass transfer model for virus
      transport  in soils.   Wat.  Res.  14:783-790.

Weast, R. C.  1986.  Handbook of Chemistry and Physics.   CRC Press, Boca Raton,
      Florida.

Yates, M. V., and S. R. Yates.   1988.  Modeling microbial fate  in the subsurface
      environment.   CRC Crit.  Rev.  Environ. Contr.       17:307-344.

Yates, M. V.  and S. R. Yates.   1989.   Septic tank setback distances: a way to
      minimize virus contamination of drinking water.  Ground Water  27:  202-
      208.
                                       65

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              APPENDIX I: SOLVING THE VIRUS TRANSPORT EQUATIONS
      The finite difference formulations used for solving  the  virus transport
equation were:
                             A(z,t)dzdt *
                                      66
                                                                           (2)

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          APPENDIX II:  Definitions of Mathematical Symbols and Units

                        For  Equations  (28)  Through (36)
Symbol


 <*.ir


 a«oil


 °w«t«r


 °tort


 finrp
     Meaning

 Albedo of air

 Albedo of soil surface

 Albedo of water surface

 Tortuosity of the soil

 Vaporization of water due
 to wind speed

 Loss  of  heat  from soil due
 to wind speed

 Specific  heat of water

 Molecular diffusion
 coefficient of water
 vapor in air

 Maximum value  of the
 dispersion coefficient of
 water vapor in the boundary
 layer of the atmosphere
   Units

 dimensionless

dimensionless

dimensionless

dimensionless

 hr cm"1


 hr cm"1


cal g"1 "CT1

 cm2 hr"1



cm2 hr"1
  •'•to
  air
Logistic representation of
the boundary layer wind speed
dependent coefficient of
dispersion of water vapor
                     Saturated vapor pressure  of
                     of the air

                     Soil porosity
                      Emissivity of the air
                      above the soil
                                                       cm2 hr
                                                             -i
                                 mm Hg
                                 cm3 soil voids
                                  cm"3 soil

                                 dimensionless
                                       67

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Symbol
     Meaning
                                                           Units
 c»oil
Porosity of the soil
at  the  atmosphere-soil
interface  soil

Emissivity of the soil

Emissivity of the water

Stefan-Boltzman constant


Infiltration of rainwater

Latent  heat of evaporation
                                                      cm3 soil
                                                      voids  cm'3
dimensionless

dimensionless

cal hr"1 cm"2
°C"*

cm hr"1

cal g"1
 n,v
 Transfer of heat by
 conduction  through
 the  soil  particles

 Transfer of heat by conduction
 and  convection in the  liquid
 phase water

 Transfer of heat by
 conduction  in the vapor
 phase water and by transport
 in the  form of latent  heat

 Relative  humidity
 at the  atmosphere-soil
 interface

 Thermal conductivity of
 the  air

 Maximum value of the
 effective thermal
 conductivity of the air

Thermal conductivity of
 the  solid

 Thermal conductivity of
 the  water

 The  effective thermal
 conductivity of the air at
 the  boundary of atmosphere-soil
 interface
                                                       cal cm"2  hr"1
                                                       cal cm"2 hr"1
                                                       cal cm"2 hr"1
                                                       dimensionless
                                                       cal cm"1
                                                       hr"1 °C"1

                                                       cal cm"1
                                                       hr"1 "C"1
                                                      cal cm"1
                                                      hr"1 "C"1

                                                      cal cm"1
                                                      hr"1 °C"1

                                                      cal cm"1
                                                      hr"1 "C"1
                                       68

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Symbol
     Meaning

Relative  humidity at the
 atmosphere-soil interface

Water  content at the
 atmosphere-soil interface

Density of water
    Units

dimensionless
                                                        cm3 cm"3
                                                        g cm
                                                            -3
  •at
      (T0)
 Density of water vapor at
 saturation at Tn
 g cm
                                                             -3
 Qh«atin
Heat flux into the  soil
 surface by rainwater
cal cm'2 hr"1
                      Heat  flux out of the soil
                       surface due to evaporation
                       or heat flux into the soil
                       surface due to condensation
                                  cal cm"2 hr"1
 1htl»wr
Heat flux into the  soil
 surface by short wave
 radiation
                                                        cal cm"1 hr"1
 Qhtssl
Heat  flux through the soil
 surface via sensible heat
                                                        cal cm"2  hr"1
 QhUwra
Heat flux into the  soil
 surface by long wave
 radiation
                                                        cal cm"2 hr"1
 
-------
Symbol                    Meaning                         Units

 T0                   Temperature at the atmosphere    °C
                      -soil interface

 T,                   Temperature at the air           °C

 WS                   Wind speed                       cm hr'1

 6z                   Thickness of boundary layer      cm
                      at the atmosphere-soil
                      interface

 z                    Soil depth                       cm
                                      70

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                       APPENDIX III.  VIRTUS USER MANUAL
      This appendix  describes  the computer program, VIRTUS, developed from the
mathematical  model described  in the  main body of the report.   The  model was
written to be run on an IBM XT, IBM AT or equivalent computer, which has 6AOK
memory and an 8087 math co-processor.  This document begins with a description
of the  program.  A  listing of FORTRAN variables  for  input with mathematical
symbols, meanings, and units is given next.  This is followed by a section on the
preparation of input data  files.  The user is  then guided through a section on
the running of the program and a discussion of the output files.

PROGRAM DESCRIPTION
Program Organization
      The program is written  in ANSI Standard FORTRAN  77.   It implements the
mathematical  model described  in the main body of this  report.   The "include"
feature of  Microsoft FORTRAN  (version 5.0)  is used  for global Common Blacks.
There is  one  "include"  file name:  "COMMON.NEW"  in the  program.   There are 12
FORTRAN source files,  all  with the  extension ".FOR".
Flow Chart of the Program
      Figure  1 shows the flow chart  illustrating the modules in the execution of
the  program.    Each  module contains many helpful  comments  and  is  compiled
separately, then the resulting object codes are  linked together to create an
executable  file called MIXMAIN.EXE.  The  functions  of  each of the modules is
described below.
Module                         Functions
MIXMAIN.FOR                    MAIN  Program MIXMAIN
MIXREAD.FOR                    Reads in simulation input data
MIXUNCS.FOR                    Computes "universal porous media constants"
MIXWRTO.FOR                    Prints  out input  system  parameters,  calculated
                               system  parameters,  and initial distributions of
                               water,  temperature, and viruses so that the user
                               knows what  the run  parameters are
MIXLRZV.FOR                    Makes decisions  about  starting and ending water
                               application and  light  cycles
MIXING.FOR                     Makes approximate time integrations of the water,
                               heat, and virus  systems
                                      71

-------
MIXWATR.FOR
MIXHEAT.FOR
MIXTHOM.FOR
MIXBNDY.FOR
MIXVIR.FOR
MIXCUM.FOR
Approximates soil water content distributions
Approximates soil temperature distributions
Solves resulting  tri-diagonal systems  of  water
and  heat  transport  equations  (and later  virus
transport equation)
Calculates all water- and heat-related variables
Approximates virus concentration distributions
Stores and prints simulation results
PROGRAM VARIABLES
      This section  contains  a  list of the program variables that  are  used in
input.    The variables  are  listed  in  alphabetic  order  (Table  Al).    The
mathematical  symbol corresponding  to each variable  is  shown,  with  a  brief
description of the meaning of the variable and its units.
DATA FILES
Format Instructions
      To run the program, the data file must be created in proper format.   The
user can create and modify the data file using a text editor or word processor.
A data file must be  created in ASCII format using the following standard FORTRAN
formatting instructions.
  Record  1             FORMAT (all of data files use
                                free format).
              TO        Initial time for the simulation
                        (usually set to zero).
              TCUT      Ending time for the simulation.
              DTO       Time step for the simulation.
  Record  2   PRTIN(I)  Nonevent print interval.  The program
                        can print out the simulation results
                        at any time according to the data given
                        here.
  Record  3   NPRINT(I) Index for print out.  See example given
                        below for more information.
  Record  4   NNSTRZ(I) Number of storage node indexes.  These
                                      72

-------
Record  5   NSLZM1

Record  6   DELTAZ

Record  7   DZ(I)


Record  8   ALBAIR


            ALBWAT


            ALBSOI


Record  9   EMSAIR


            EMSWAT


            EMSSOI


Record 10   LAMBHT
Record 11

Record 12
Record 13
Record 14
LAHSLD

SHTSAN

SHTSIL

SHTCLA

SHTWAT

SHTAIR

RHOSND

RHOC1A

RHOSIL

GAMTLI
indexes are used to print out simulation
results at a given time and soil depth
for checking the program.

Number of internal vertical soil nodes.

Top boundary layer thickness.

Soil depth step.  See example given
below for more information.

Albedo of soil surface air which is used
for heat flux at the top boundary.

Albedo of water which is used for heat
flux at the top boundary.

Albedo of soil which is used for heat
flux at the top boundary.

Emissivity of air which is used for
heat flux at the top boundary.

Emissivity of water which is used for
heat flux at the top boundary.

Emissivity of soil which is used for
heat flux at the top boundary.

Coefficient due to wind speed affecting
on thermal conductivity of air.

Thermal conductivity of solids.

Specific heat of sand.

Specific heat of silt.

Specific heat of clay.

Specific heat of water.

Specific heat of air.

Density of sand.

Density of clay.

Density of silt.

Derivative of surface tension with
respect to temperature.
                                     73

-------
            BETATV
Record 15   NWVAIR
Record 16   DWVAR
Record 17
Record 18
RHOWAT

RHOAIR

WS
Record 19   NCFTHP
            NCFFRH
Record 20   ATEMP(I)
Derivative of saturation water vapor
density with respect to temperature.

Number of characterizing parameters
for the effective water vapor
diffusivity in the top boundary
layer.  See example given below for
aore information.

Effective water vapor diffusivity
parameters in the top boundary layer.
See example given below for more
information.

Density of water.

Density of air.

Vind peed at the atmosphere-soil
surface.

Number of coefficients in the Fourier
Series (equation 56) representing nth
term of air temperature.

Number of coefficients in the Fourier
Series (equation 57) representing nth
term of relative humidity.

Temperature coefficients in equation
(56).  These values are generated by
fitting the daily air temperature data
to equation (56).  See Table 1 to
identify the correspond mathematical
symbol in equation (56).

Temperature coefficients in equation
(56).  These values are generated by
fitting the daily air temperature data
to equation (56).
            OMEGTP(I) Temperature coefficients in equation
                      (56).  These values are generated by
                      fitting  the daily air temperature data
                      to equation (56).
            BTEMP(I)
Record 21   ARHIN(I)
          Relative humidity coefficients in
          equation (57).  These values are
          generated by fitting the daily relative
          humidity data to equation  (57).  See
          Table  1 to  identify the correspond
                                     74

-------
                      mathematical symbol in equation (57).

            BRHIN(I)  Relative humidity coefficients in
                      equation (57).  These values are
                      generated by fitting the daily relative
                      humidity data to equation (57).

            OMGRHI(I) Relative humidity coefficients in
                      equation (57).  These values are
                      generated by fitting the daily relative
                      humidity data to equation (57).

Record 22   TPINMU    Mean  air temperature in equation (56).

            RHINMU    Mean  relative humidity in equation (57).

            TPWAT1    Temperature in rainwater.

Record 23   CWIN      Concentration of virus in infiltration
                      water.

            CVGRD     Concentration of virus in ground water.

            DLO       Diffusion coefficient of virus.

            DISPLZ    Dispersivity coefficient of virus.

            KD        Virus adsorption coefficient.

            FILTRA    Filtration coefficient of virus.

            THTAST(J) Saturated soil water content at each
                      node  of soil.  These values are
                      generated by fitting the experimental
                      data  (water content vs. water potential)
                      to  equation (59).

Record 24   TORT(J)   Tortuosity factor of soil.

Record 25   EPS(J)    Soil  porosity.

Record 26   PCTSAN(J) Percentage of sand.

Record 27   PCTCLA(J) Percentage of clay.

Record 28   PCTSIL(J) Percentage of silt.

Record 29   ALPTH(J)  Parameter in equation (59).  The values
                      are generated by fitting the experimen-
                      tal data to equation (59).

Record 30   BETATH(J) Parameter in equation (59).  The values

                                    75

-------
Record 31   GAMCNS(J)
Record 32   KTHSTS(J)
Record 38
Record 39
Record 40
Record 41
Record 42
          are generated by fitting the experimen-
          tal data to equation (59).

          Parameter in equation (60).   The values
          are generated by fitting the experimen-
          tal data to equation (60).

          Saturated conductivity in equation (60).
          These values are generated by fitting
          the experimental data to equation (60).
Record 33   THTRES(J) Residual soil water content.

Record 34   DALPTZ    Derivative of at with respect to soil
                      depth z.  See example given below for
                      more information.
Record 35   DBETAZ
Record 36   DTHTSZ
Record 37   DTHREZ
THTAA

TEMPA

THTAS(J)

TEMPS(J)

CV(J)

QRAIN(J)



QSR(J)



NLIEV

NRAEV

TLION(I)

TLIOF(I)
Derivative of f)e with respect to soil
depth z.  See example given below for
more information.

Derivative of saturated water content
with respect to soil depth z.

Derivative of residual water content
with respect to soil depth z.

Initial air relative humidity.

Initial air temperature.

Initial soil water content.

Initial soil temperature.

Initial soil virus concentration.

Parameters for rainfall rate.  See
example given below for more
information.

Parameters for solar intensity.  See
example given below for more
information.

Number of light events.

Number of rain events.

Time at which the light is turned on.

Time at which the light is turned off.

              76

-------
  Record A3    TRAON(I)  Time at which the rain is turned on.
               TRAOF(I)  Time at which the rain is turned off.
Examples  of Data Sets
      Examples for input data files are presented and described in this section.
These  data files,  together with  the  executable program, are  included on the
distribution diskette.  It is recommended that these files be used as input to
the program to verify that the program is working properly.  These files can be
modified  to describe an actual scenario  for model simulation.
Example Input Data File: WTV1.DAT
      This section discusses  the run  control,  upper boundary,  and thermal
properties input data that are in  the  file: WTV1.DAT.  These data are  read  in by
the subroutine MIXREAD in nodule MIXREAD.FOR.   The data used by  the program are
numerical, in  either  real or  integer form.   Real numbers are  handled by the
program as double precision.  The data is read using FORTRAN'S  "list-directed"
input  format.   This  is  a free form  input:  numbers may  occupy any  number of
positions; they are separated by spaces.   The  following are the  descriptions of
the  actions  of MIXREAD for  the  input file WTV1.DAT.   The  input data and the
corresponding FORTRAN codes  are first listed, then discussed briefly.
      As  the program begins  to execute,  the following  message
    •E««din« IroB d«t« file WTV1.DAT'
is shown  on the screen.   It tells  the  user that the program is  beginning to read
in data in the file: WTV1.DAT.  Then it reads  in the  run control information in
the first line of WTV1.DAT as follows:
    o.o  2.1   o.i
    «EAD{2,*) TO.TCUT.DTO
It first  reads in the time  for beginning the simulation,  then the time  for ending
the simulation, and finally the time step for running the simulation.  The time
step may  vary for simulations. The user  should try several time steps to decide
which  is  required for the  purpose of  achieving  the desired accuracy.
     i.o 5.BO 11.ee i7.es 23.ee ze.ee 3S.ee *i.se *7.ee 7i.ee ei.ee
     lie.99 ise.ee i48.es iss.es ise.es i7s.se

                                        77

-------
     KEADC2,*) (F&TINU). 1-1,16)
The  program reads  in the number of  times for printing  the  simulation results.
The  first number in the above data file  is  the  time for printing the simulation
results in output file:  "test.out".   The  other numbers  are  times for printing the
simulation results in output file:  "plrnfl".  In order  to print the results at
6 hours, the number 5.99 is used.  This  is  to avoid possible numerical roundoff
which may cause the program to  print the results before or  after 6 hours.
    00000000101010000010
    SEADC2,*) (IPRIHTCI),1-1,20)
The  program reads in the  index for the  print  out.   The  user may  not have to
change these numbers.
    1  2  3 4 5  8  7  8  9 10 11 12 13 1* 13 16 17  16 IB 20 21 22
    23 24 25
    X£AD(2,«)(HKStR2(I),1-1.25)
The  program reads  in the  number  of  storage  node  indexes  for the  output file:
TEST.OUT.  In this example, the simulation results of the top 25 nodes for every
hour are saved in the file of TEST.OUT.
    89
    K£AD(2.*) HSLZM1
    HSLZZZ-NSLZMl+1
    XSLZF1-HSLZZZ+1
First, the number of  internal vertical soil nodes  is  read in.   Then, the upper
and  bottom nodes are  computed.
    i.o
    o.i
    i.o
    i.o
    i.o
    KEADC2.*) DELTA!
    DO 16, I-l.HSLZZZ
    EEAD(2,«) DZ(I)
 IB CONTINUZ
The program reads  in the upper boundary layer  thickness and  distance between
nodes.  The depth step  in this example is 1 cm although  it  is  not necessary to
have  equal space for all depth steps.  However, it is recommended that the first
•oil  layer  (DZ(l)) should  be  kept  small  (0.1)  for  the purpose  of obtaining
accurate  results.
    o.os     0.07    o.oe
    0.90     0.9$    0.50
                                        78

-------
    3.33000E-4
    3.43
    0.175    0.175    0.175     1.0      0.2*

    SEAD(2,«) AIAAIR.ALBWAT.ALBSOI
    XEADC2.*) mSAJR.EMSWAT.BCSOI
    HEADC2.*) LAMEST
    IEADC2.*) LAtCLD
    *£AD<2,«> SBTSAH.SETSIL.SBTCLA.SBnUT.SHTAIR


The program reads  In the albedos,  emissivities,  thermal  conductivities,  and

specific  heats of air,  water,  and soil.   All  these  coefficients  may  be kept

constant  except the albedo coefficients  of soil (ALBSOI). which may  change  for

different types of  soil.

     2.66     2.64     2.65

     KEADC2.*) RHOSND.RBOCLA.RBOSIL


The program reads in soil particle densities, which are always  constant.

     -2.0900E-3 1.05600E-6
     3
     954.0     4770.0   3.33000E-4

     HEADC2,*) GAMTLI.BETATV
     KEAD(2.*) HWVAIR
     BEAD (2. • ) (DWVAR (I). 1-1, FWVAIR)


The program reads in liquid  and vapor phase water parameters.   These  parameters

may be  kept constant for  all the  simulations.

     i.o 0.0011

     SEADC2.*) RHOHAI.HBQAIR

The program reads in the densities  of water and air, which  are always constant.

     o.o

     SEAD(2.*> MS


The program reads in  wind speed  constant.   It  is assumed  to be  zero  in this

example.

     2  5
     -1.006722008  -2.356626766    0.261188
     -0.371635705  1.000000000     0.523598
     0.0371929656  0.0675873369    0.261198
     -.0052916350  -.0274241549    0.523598
     -.0094577665  0.0116668147    0.785367
     O.C0637S2911  -.0043302556    1.047196
     -.0061084983  0.0040792275    1.308895

     READ<2.*> BCFTMF.IICFFBB
     DO 13. I - l.HCFTKP
        HEAD(2.«) ATEMPCD.BIEMFCD.CHEGTPd)
   13 COHTimjE
     DO 23, I - l.KFFKB
        K£AD(2.*> ARHIN(I).BREIM(I),OMQRBI(I)
   23 COHTIHUE
                                             79

-------
The program reads in the number of air temperature coefficients (in equation 56)
and  relative humidity  coefficients  (in  equation 57).   These coefficients  are
generated by fitting the measurement data to equations (56) and  (57).
     8.7578      0.869        277.0
     BZAD<2,«) TPnWU.BBHWU.TFWATI
The  first two variables are the mean air temperature in equation  (56) and  mean
relative  humidity in equation (57), respectively.   The third variable is  the
temperature in the applied water.
Example Input Data File: WTV2.DAT
      This section discusses the virus transport properties input data in file:
VTV2.DAT.   It  is read  in by subroutine MIXREAD in module MIXREAD.FOR.    The
following are the  descriptions of  the  actions  of  MIXREAD for  the input  file
WTV2.DAT.
      As  the  program begins to execute,  the  following message
    •R»«ding from file WTV2.DAT1
is shown on the screen.  It tells the user that the program is  beginning  to  read
data in the UTV2.DAT file.  Then,  it reads in the virus transport  properties as
follows:
     l.OE+5  0.0 0.00032*  200.0  0.27 0.0
     SEADC3,*)  CHIN, CVGRD, DLO, DISPLZ, KD, FILTRA
Table 1A  lists the definitions  and units of the above  FORTRAN variables.   The
user may  need to change  the  values of  the  variables according to a specific
simulation.
Example Input Data File: WTV3.DAT
      This section discusses  the  soil  physical properties  input data in file:
VTV3.DAT.   It  is read  in by subroutine MIXREAD in module MIXREAD.FOR.    The
following are the  descriptions of  the  actions  of  MIXREAD for  the input  file
WTV2.DAT.
      As  the  program begins to execute,  the  following message
     •R«*din( Iron file  WTV3.DAT1
is shown on the screen.  It tells the user that the program is  beginning  to  read
data  in WTV3.DAT file.   Then,  it  reads in the soil physical properties  as
follows:
     0.55    0.55    0.55    0.55   0.55    0.55    0.55
     0.55    0.55    0.55    0.55   0.55    0.55    0.55
     0.55    0.55    0.55    0.55   0.55    0.55    0.55

                                       80

-------
0.55
O.SS
0.55
0.55
O.SS
0.55
0.55
0.55
O.SS
O.SS
0.55
O.SS
O.SS
0.55
O.SS
O.SS
O.SS
O.SS
O.SS
O.SS
O.SS
O.SS
e.ss
O.SS
O.SS
0.35
O.SS
O.SS
O.SS
O.SS
O.SS
O.SS
0.55
O.SS
O.SS
O.SS
0.55
0.55
O.SS
O.SS
0.55
0.55
0.55
0.55
O.SS
0.55
O.SS

0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55

0.55
0.55
0.55
O.SS
0.55
0.55
0.55
0.55
O.SS
0.55
0.55

0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55

     READ(*,*>(THTAST(J),J"1,ISL2P1)
The progran reads  In the saturated soil water content  at each node of the soil
profile.   The saturated  soil water content varies for different types of soil.
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6500
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
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.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600

.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600

.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600

.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600

  B£AD(4.«HTORICJ),J-1.HSLZ!>1)
The program reads in the  tortuosity factor at  each node of  the  soil profile
These  values nay  be  kept constant for most soils  (Hillel,  1982).
0.55
0.55
0.55
O.SS
O.SS
0.55
0.55
O.SS
0.55
O.SS
O.SS
O.SS
0.55
0.55
O.SS
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
e.
0.
0.
0.
0.
55
55
55
55
55
55
55
55
55
55
55
55
55
55
35
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
55
55
55
55
55
55
55
55
55
55
55
55
55
55
55
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.35
.55
.55
.55
.55
0
0
0
0
0
0
0
0
0
0
0
0
0
0

.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.35
.55

0
0
0
0
0
0
0
0
0
0
0
0
0
0

.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55

0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
O.SS
O.SS
0.55
0.55

    READ(4.•)CEPSCJ),J-1,HSLZP1)
The progran reads in the  soil porosity at each node of the soil profile.  These
values  vary for different types of soil.
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
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0.3
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0.3
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0.3
0.3
                                        81

-------
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3

0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3

0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3

    BEAD(4,•)(KTSAft (J),J-l.BSLZF1)
The program reads in the percentage  of sand at each node of  the soil profile.
These values are extracted from experimental measurements.  If  the values are not
available  for a specific soil, the user can estimate them easily through the soil
textural triangle diagram, which is commonly shown in the soil science text book
(Brady.  1984).
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
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.1000
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.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

  mEAD(*.*)(PCTCLA(J),J-l,HSLZPl)
The program reads in the percentage  of clay at each node of  the  soil profile.
As with  the percentage of sand, if the values  are not  available for a specific
soil,  the  user  can estimate  them easily  through the  soil  textural triangle
diagram.
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
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0.6
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0.6
0.6
0.6
0.6

0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6

0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6

    SEAD(*.«)(PCTSIL(J),J-
The program reads in the percentage  of silt at each node of  the soil profile.
                                        82

-------
Again, if the values are not available for a specific soil, the user can estimate
them easily  through the soil  textural triangle  diagram.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241.
     1241
     1241
     1241
39 1241.39
39 1241.39
39 1241.39
38 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.
1241.
1241.
1241.
1241.
1241.
1241.
1241.
1241.
1241
1241.
1241.
1241.
1241.
39 1241.
39 1241.
39 1241.
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
39 1241.39
.39
.39
.39
    BEAD(4,«)(ALPTH(J).J-l,HSLZP1)

The program reads  in the  parameter  in equation (59)  at  each  node  of the soil

profile.  These values are generated by fitting the experimental data to equation

(59).    The  user  can  identify  the   FORTRAN  variable  to  the   corresponding

mathematical symbol using Table  1A.
7079
7079
7079
7079
7079
7079
7079
7079
7079
7079
7079
7079
7079
7079
7079
.7079
.7079
.7079
.7079
.7078
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7078
.7079
.7079
.7079
.7079
.7079
.7078
.7079
.7076
.7079
.7079
.7079
.7079
.7078
.7078
.7079
.7079
.7079
.7079
.7079
.7079
.7078
.7078
.7078
.7078
.7079
.7079

.7079
.7079
.7079
.7078
.7078
.7078
.7078
.7078
.7078
.7078
.7078
.7078
.7079
.7078

.7079
.7078
.7079
.7073
.7078
.7078
.7078
.7078
.7078
.7079
.7078
.7079
.7078
.7078

.7078
.7078
.7078
.7078
.7078
.7078
.7079
.7079
.7078
.7079
.7079
.7079
.7078
.7078

  SEAD(4,«)(BrTATH(J).J-l.KSl,ZPl)
The program reads  in the parameter  in  equation (59)  at  each  node  of the soil
profile.  These values are generated by fitting the experimental data to equation

(59).
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.3
.5
.5
.5
.5
.5
.3
.5
.5
.5
.5
.5
.3
.5
.5
.5
.5
.3
.5
.5
.5
.5
.5
.5
.5
.5
.5
.3
.3
.5
.5
.3
.5
.5
.5
.5
.5
.5
.5
.5
.5
.3
.3
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.3
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
                                              83

-------
    6.S    6.5    6.5    6.5
    «EAD (*. • ) (GMC8S (J). J-l. HSLZP1)
The program reads  In the parameter  In equation  (60)  at each node  of the soil
profile.  These values are generated by fitting the experimental data to equation
(60).
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61 1
0.61 I
0.61 1
0.61 1
0.61 1
0.61 1
.61
.61
.61
.61
.61
.61
.61
.61
.61
3.61
).61
).61
3.61
3.61
3.61
0.61
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0.61

0.61
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0.61
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0.61
0.61
0.61
0.61
0.61
0.61
0.61

    «EAI>(4.*>(lCTHSTS(J),J-l,irSLZFl)
The program reads in the saturated conductivity at each node of the soil profile.
These values are generated by fitting the experimental data  to  equation (60).
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
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0.029
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0.029
0.029
0.029
0.029
0.029

0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
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0.029
0.029
0.029
0.029

0.029
0.029
0.029
0.029
0.029
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0.029
0.029
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0.029
0.029
0.029
0.029
0.029

0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029

  SEADC*.*)(IHTRES(J).J-l.HSLZP1)
The program reads in the residual soil water content at each  node of the soil
profile.  These values are generated by fitting the experimental data to equation
(59).
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
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0.0
0.0
0.0
0.0
                                        84

-------
0.0
0.0
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O.D
0.0
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o.o
0.0
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0.0
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0.0
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0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

    12AD(4,*)(DAI.PTZ(J).J-1,IISI.ZP1)
    BEAD(4,*)(DBtTAZ(J),J-l.HSLZP1)
    READ(4.*>(DTHTSZ(J).J"1,HSI.ZP1)
    BZAD(4.«)(DTHREZ(J),J-1,KSLZF1)


The program reads in the derivatives of  parameters in equations (59) and  (60)

with  respect to soil depth.  Since these parameters are  assumed to be constant

throughout the  soil  profile, the derivatives of parameters with respect  to  soil

depth should be all  zero.  The user may not have to change them.

Example Input Data File: WTV4.DAT

       This section discusses the initial distributions  of  water, temperature, and



                                         85

-------
virus Input  data in  file:  WTV4.DAT.   It  is  read in by subroutine  MIXREAD in

nodule MIXREAD.FOR.  The following are the descriptions of the actions of MIXREAD

for the input  file  WTV4.DAT.

       As the program begins to execute,  the following message

     •R»»dim frca til* HTV4.DAT'

is  shown on the screen.  It tells the user  that  the program is beginning to read

data in WTV4.DAT  file.

     0.092    281.72

     KEADCS.*) THIAA.TEMPA


The program reads in the initial  relative humidity  and air temperature  at the

upper boundary layer.  The user may change these values according to the location

and beginning  season.

     0.25     281.72  0.0
     0.25     281.72  0.0



     0.25     281.72  0.0
     0.25     281.72  0.0

     DO 127. J-l.KSLZPl
       READCS,*) THTAS(J>,TBC'S(J),CV(J)
  127 CONTINUE


The program reads in the initial  soil water  contents,  temperatures,  and virus

concentrations.

Example Input Data  File:  WTV5.DAT

       This section  discusses the rainfall rate, solar radiation, .rain and light

cycles input data in file:  WTV5.DAT.   It  is  read in by subroutine  MIXREAD in

module MIXREAD.FOR.  The following are the descriptions of the actions of MIXREAD

for the  input file  WTV5.DAT.

       As the program begins to execute,  the following message

     •lUadias free. til. WTV5.DAT1

is  shown on the screen.  It  tells  the  user that the program begins to read data

in  WTV5.DAT file.

     0.0      0.0
     0.1      35.607219
     0.0      6.6392*47
     0.0     2.5100518
     0.0      0.15

     00 15, 1-1,5
       KEADC6.*) ORAIHCI),QSRU>
  15 CONTINUE


The program reads  In  the  parameters  for the  rainfall rate and  the  solar


                                        86

-------
intensity.  The value in the second row of column one above is the rainfall rate.
The values  in the second to fourth rows of column two are for the parameters in
equation (58).  The value in the fifth row of column two is the solar intensity
factor  during the rainy period,  which is assumed to be  15%  of the total solar
intensity AS  compared to that of no rain.  Other zero values are control indexes
which the user nay not have to change.
     s       2
     KEADC6.*) K.IZV.NKAEV
The program reads in the numbers of sunrise  and sunset cycles  and rain events .
     e.o    le.o
     30.0    42.0
     54 . 0    66 . 0
     76.0    90.0
    102.0    114.0
     DO 16. I-l.Hl.IEV
       HZAD(6,*) TLIOH(I),TLIOF(I)
  16 CONTINUE
The program reads in the times for sunrise and  sunset.
     o.o     e.o
    130.0    140.0
     DO 17. I-l.KRAEV
       EEADC6.*) IRAON(I),TRAOF(I)
  17 CONTINUE
The program reads in the times for rain  start and rain stop.
RUNNING THE PROGRAM AND OUTPUT FILES
Instructions  for Running the  Program
      The program can be run  on  IBM PC,  IBM  AT  computer, or their equivalents,
which have  640K memory  and  an 8087 math co-processor.  A hard disk is desirable,
but not absolutely  necessary.   The  program was developed under DOS 4.0, using
Microsoft FORTRAN Version 5.0.  The distribution  diskette includes the executable
form of the program (MIXMAIN.EXE), input  data files,  and output files.
      There is one Batch file on the  distribution diskette:  MIX.INP, which is
used to run the  program. To start running the program, the user may just type:
If the user wants to type the input  files  from the screen,  s/he may just type:
C:\XGXMMH
at this point, the computer will ask  for one output file (see next section about
the output  files), five  input files,  and two other  output files consecutively by
displaying  on the screen.
                                        87

-------
      When the program Is run, it will display information on the screen.  First,
it will show  the messages  about  the  reading of input data,  the writing of all
input data, the convergence  checking (those ".  .  .  ."on the screen), and the
times.   If the program  should stop  due  to a  data  error,  or  for  some other
unexpected reason, the last  messages displayed will  give an indication of the
program that caused the error.  If the program  runs well,  it will show how much
computing time is used at the end of running.
Output Files
      File TEST.OUT shows the information read fromWTVl.DAT, WTV2.DAT, WTV2.DAT,
WTV3.DAT, WTV4.DAT, AND WTV5.DAT, together with computed data.  This information
should be examined to be  sure that the data were read  in correctly.   All the
numbers  are  labeled  to  indicate what  they represent.    Next, it  shows  some
simulation results  at each  hour,  including  simulation  time,  water  content,
temperature,   virus  concentration,  water  potential,  conductivity,  water  flow
velocity, and  the upper boundary parameters.   This  information should also be
examined to be sure the right simulation results were generated mathematically
and scientifically.
      File P1RNFL shows water content, temperature,  and virus concentration at
all of the node points in  the soil profile  at  selected  print times.  They are
used to draw graphs.
      File P2RNFL shows soil  temperature  and  surface evaporation  rate at each
hour at the selected soil depths.  They are also used to draw graphs.
                                      88

-------
Table Al.  Program Variables for Input Data
FORTRAN Hath
Variable Symbol
ALBAIR a.ir
ALBSOL o.oil
ALBWAT a,,,.,
ALPTH at
ARHIN A,,,

ATEMP At.

BATATH fig
BETATV dpj;1 /dT

BRHIN Brh

BTEMP BtM

CV G!

CVGRD Cg

CWIN C^

DALPTZ da,,/dz

DBETAZ d0v/dz

DELTAZ 6z
DISPLZ 0^.,,
DLO DXO
DTO At
Meaning
Albedo of surface air
Albedo of surface soil
Albedo of surface water
Parameter for equation
Unit



(59) cm
Relative humidity coefficient in
equation (57)
Temperature coefficient
(56)
Parameter for equation
Derivative of saturated
density with respect to

in equation

(59)
water vapor g/cm3 °K
temperature
Relative humidity coefficient in
equation (57)
Temperature coefficient
(56)
Concentration of virus

Concentration of virus
water
Concentration of virus
infiltration water

in equation

in soil water mass cm"3
water
in ground mass cm'3
water
in mass cm"3
water
Derivative of ae with respect to
soil depth z

Derivative of 0e with respect to
soil depth z

Upper boundary layer thickness cm
Dispersivity coefficient of virus cm
Diffusion coefficient of virus cm2/hr
Time step for running the model hr
                     89

-------
           Table Al.   Program Variables for Input Data (continued)
FORTRAN
Variable
          Math
          Symbol
     Meaning
Unit
DTHREZ
DTHTSZ
DWVAR
DZ
EMSAIR
EMSSOI
EMSWAT
EPS
FILTRA
GAMCNS
Az
«ar
c»oil
£w«t«r
c
f
7C
GAMTLI   d(surf.
      tension)/dT

KD        K^
KTHSTS

LAMBHT


LAMSLD


NCFFRH
NCFTMP
Derivative of 6t with respect  to
soil depth z

Derivative of 0, with respect  to
soil depth z

Effective water vapor diffusivity
parameters in boundary layer

Depth step of soil profile

Emissivity of air above soil

Emissivity of soil surface

Emissivity of water

Soil porosity

Filtration coefficient of virus

Parameter for equation (60)

Derivative of surface tension with
respect to temperature

Virus adsorption coefficient


Saturated conductivity of soil

Coefficient due  to  wind speed
effects on thermal conductivity of air

Thermal conductivity of solids
Number of  coefficients  in the
Fourier series  (equation 56)
representing n"1 term of air
temperature

Number of  coefficients  in the
Fourier series  (equation 57)
representing n** term of relative
humidity
                                                                 cm
                                                                 cm3/cm3

                                                                 I/cm
ml/g
 soil

cm/hr
cal/cm
 hr °K
                                     90

-------
Table Al.  Program Variables for Input Data (continued)
FORTRAN Math
Variable Symbol
NLIEV
NNSTRZ
NPRINT
NRAEV
NSLZK1
NWVAIR
OMEGTP u^
OMGRHI wjn
PCTCLA
PCTSAN
PCTSIL
QRAIN
QSR
RHINMU RH
RHAIR ,.ir
RHOCLA pcl.y
RHOSIL P«iit
RHOSND p>Kld
RHOWAT *W.r
SHTAIR c^r
SHTCLA ceUy
SHTSAN c.md

Meaning Unit
Number of light events
Storage node indexes
Indices for print out
Number of rain events
Number of internal vertical soil
nodes
Number of characterizing parameters
for the effective water vapor
diffusivity in the boundary layer
Parameter in equation (56)
Parameter in equation (56)
Percentage of clay
Percentage of sand
Percentage of silt
Parameter for rainfall rate cm/hr
Light flux charactering parameters cal/cm2
hr
Parameter in equation (57)
Bulk density of air g cm'3
Bulk density of clay g cm'3
Bulk density of silt g cm"3
Bulk density of sand g cm'3
Bulk density of water g cm"3
Specific heat of air cal/g °K
Specific heat of clay cal/g °K
Specific heat of sand cal/g °K
91

-------
           Table Al.  Program Variables for Input Data (continued)
FORTRAN Math
Variable Symbol
SHTSIL c.ut
SHTWAT cv.^
TO
TCUT
TEMPA T^r
TEMPS T
THTAA 0mir
THTAS 8
THTAST 6.
THTRES 6;
TLIOF
TLION
TORT otort
TPINMU T
TPWATI
TRAOF
Meaning
Specific heat of silt
Specific heat of water
Start time for simulation
End time for simulation
Initial air temperature
Initial soil temperature
Initial air relative humidity
Initial soil water content
Saturated soil water content
Residual soil water content
in equations (59) and (60)
Time at which the light is
turned off
Time at which the light is
turned on
Tortuosity factor of soil
Parameter in equation (56)
Temperature of rain water
Time at which the rain is
Unit
cal/g °K
cal/g °K
hr
hr
°K
°K

cm3/cm3
cm3/cm3
cm3/cm3
hr
hr

°K
°K
hr
TRAON
turned off

Time at which the rain is
turned on
hr
US
Vind speed at the atmosphere-
soil surface
                                                                 cm
                                     92

-------
                APPENDIX IV: LISTING OF INPUT AND OUTPUT FILES

Input Data File: vtvl.dat

0.0   6.1    0.1
1.0  2.99 5.99 17.99 23.99 29.99 35.99 41.99 47.99 71.99 95.99
119.99 139.99 149.99 159.99 169.99 179.99
 00000000101010000010
  1  2  3  4  5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22
23 24 25
99
1.0
0.1
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0

                                       93

-------
   1,
   1
   1.
   1.
   1.
   1,
   1.
   1.
   1.
   1,
   1.
   1.
   1.
   1.
   1.
   1.
  1.
  1.
  1,
  1,
  1.
  1.
  1.
  1.
  1.
  1,
  1.
  1.
  1,
  1
  1.
  1.
 1.
 1,
 1.
 1.
 1.
 1.
 1,
 1.
 1.
 1.
 1.
 1.
 1.
 1,
 1,
 1.
 1,
1,
    .0
    .0
    .0
    ,0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
    .0
   .0
   .0
   .0
   .0
   .0
   .0
   .0
   .0
   .0
   .0
   ,0
   .0
   .0
   .0
   .0
   .0
   .0
  .0
  .0
  .0
  .0
  .0
  .0
  .0
  .0
  .0
  ,0
1.0
1.0
                                       94

-------
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
0.05 0
0.90 0
3.33000E-4
3.43
0.175 0
2.66 2








.07
.95


.175
.64
                    0.09
                    0.50
                    0.175
                    2.65
954.0
1.0
0.0
 2  5
-1.006722008
-0.371835795
0.0371929656
-.0052916350
-.0094577865
0.0063752911
-.0061084983
8.7578
4770.0
0.0011
                     1.0
                     0.24
-2.0900E-3 1.05600E-6
 3
3.33000E-4
    -2.356626769
    1.000000000
    0.0675873369
    -.0274241549
    0.0116668147
    -.0043302556
    0.0040792275
    0.869
          0.261199
          0.523598
          0.261199
          0.523598
          0.785397
           .047196
           .308995
1.
1.
          277.0
                                      95

-------
Input Data File:  WTV2.dat




l.OE+5  0.0  0.000324  1.0  0.27  0.0
                                     96

-------
Input Data File; WTV3.dat
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.3
0.3
0.3
0.3
0.3
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.3
0.3
0.3
0.3
0.3
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.















0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.3
0.3
0.3
0.3
0.3
55
55
55
55
55
55
55
55
55
55
55
55
55
55
55
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
55
55
55
55
55
55
55
55
55
55
55
55
55
55
55
0.
0.
0.
0.
0.
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55

.
.
t
m
f
f
.
^
t
,
f
f
f
m

0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
3
3
3
3
3















6600
6600
6600
6600
6600
6600
6600
6600
6600
6600
6600
6600
6600
6600
















0.3
0.3
0.3
0.3
0.3
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
















0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55
0.55






0
0
0
0
0
0
0
0
0
0
0
0
0
0

.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600

0
0
0
0
0
0
0
0
0
0
0
0
0
0

0.3
0.3
0.3
0.3
0.3
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
















.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55
.55

0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.

.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600

0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.

3
3
3
3
3
55
55
55
55
55
55
55
55
55
55
55
55
55
55
















55
55
55
55
55
55
55
55
55
55
55
55
55
55





















.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600
.6600





















                                      97

-------









































1241

1241

1241

1241

1241

1241
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
1241.39
.39
1241.39
.39
1241.39
.39
1241.39
.39
1241.39
.39
1241.39
.39
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.















0.
0.
0.
0.
0.
0.
0.
0.
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0.
0.
0.
0.
0.
0.












3 0.3
3 0.3
3 0.3
3 0.3
3 0.3
3 0.3
3 0.3
3 0.3
3 0.3
3 0.3
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
6 0.6
1241.39

1241.39

1241.39

1241.39

1241.39

1241.39

0
0
0
0
0
0
0
0
0
0
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0












.3
.3
.3
.3
.3
.3
.3
.3
.3
.3















.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
1241.

1241.

1241.

1241.

1241.

1241.

0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3

.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6

39

39

39

39

39

39

0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3
0.3

.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6
0.6

1241.39

1241.39

1241.39

1241.39

1241.39

1241.39

0
0
0
0
0
0
0
0
0
















0
0
0
0
0
0
0
0
0
0
0
0
0
0













.3
.3
.3
.3
.3
.3
.3
.3
.3

.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000

.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6
.6

1241.39

1241.39

1241.39

1241.39

1241.39

1241.39











.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
.1000
















1241

1241

1241

1241

1241

1241









































.39

.39

.39

.39

.39

.39

98

-------
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
1241.39
1241.39 1241.39 1241.39
,7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079 .7079 .7079 .7079
.7079
6.
6.
6.
6.
6.
6.
6.
6.
6.
6.
6.
6.
6.
6.
6.
0.
0.
0.
0.
0.
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
61
61
61
61
61
.7079
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
0.61
0.61
0.61
0.61
0.61
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6





1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
1241.39
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
.7079
1241.39 1241.39
1241.39 1241.39
1241.39 1241.39
1241.39 1241.39
1241.39 1241.39
1241.39 1241.39
1241.39 1241.39
1241.39 1241.39
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079 .7079
.7079
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
0.61
0.61
0.61
0.61
0.61
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
0.61
0.61
0.61
0.61
0.61
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5
6.5






6
6
6
6
6
6
6
6
6
6
6
6
6
6

0.61
0.61
0.61
0.61
0.61
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5






6
6
6
6
6
6
6
6
6
6
6
6
6
6

0.61
0.61
0.61
0.61
0.61
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5
.5





















0.61
0.61
0.61
0.61
0.61
99

-------
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.0
0.0
0.0
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0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0.0
0.0
0.0
0.0
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0.0
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0.0
0.0
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0.0
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0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
61
61
61
61
61
61
61
61
61
61
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029



























0.
0.
0.
0.
0.
0.
0.
0.
0.
0.















0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
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0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
61
61
61
61
61
61
61
61
61
61
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0
0
0
0
0
0
0
0
0
0
0
0
0
0

0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0










.029
.029
.029
.029
.029
.029
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.029
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0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61

0
0
0
0
0
0
0
0
0
0
0
0
0
0

0
0
0
0
0
0
0
0
0
0
0
0
0
0

0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0

.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029
.029

.0
.0
.0
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.0
.0
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.0
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.0
.0
.0
.61
.61
.61
.61
.61
.61
.61
.61
.61

0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61
0.61

029
029
029
029
029
029
029
029
029
029
029
029
029
029






































0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029
0.029




























100

-------
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
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0.0
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0.0
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0.0
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0.0
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0.0
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0.0
0.0
0.0
0.0
0.0
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0.0
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0.0
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0.0
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0.0
0.0

0.0
0.0
0.0
0.0
0.0
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0.0
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0.0
0.0
0.0
0.0
0.0
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0.0
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0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

101

-------
Input Data File: WTV4.dat
0.892
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72

0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
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0.0
0.0
0.0
0.0
                                      102

-------
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
281.72
0.0
0.0
0.0
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0.0
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0.0
0.0
103

-------
 Input  pqta  File! WTV5.dat

 0-0       0.0
 0.1       35.607219
 0.0       6.6392447
 0.0       2.5100518
 0-0       0.15
 5         2
 6.0       18.0
 30.0      42.0
 54.0      66.0
 78.0      90.0
102.0     114.0
0.0       6.0
130.0     140.0
                                    105

-------
Output Data File: P1RNFL
TIMEST- .3000E+01
LAYER

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
NODE POSITION
( CM )
.OOOOE+00
. 1000E+00
.1100E+01
.2100E+01
.3100E+01
.4100E+01
.5100E+01
.6100E+01
.7100E+01
.8100E+01
.9100E+01
.1010E+02
.1110E+02
.1210E+02
.1310E+02
.1410E+02
.1510E+02
.1610E+02
.1710E+02
.1810E+02
.1910E+02
.2010E+02
.2110E+02
.2210E+02
.2310E+02
.2410E+02
.2510E+02
.2610E+02
.2710E+02
.2810E+02
.2910E+02
.3010E+02
.3110E+02
.3210E+02
.3310E+02
. 3410E+02
.3510E+02
.3610E+02
.3710E+02
.3810E+02
.3910E+02
.4010E+02
.4110E+02
.4210E+02
.4310E+02
.4410E+02
.4510E+02
WATER
(CM3/CM3 SOIL)
.2931E+00
.2926E+00
.2889E+00
.2849E+00
.2809E+00
.2770E+00
.2731E+00
.2694E+00
.2660E+00
.2629E+00
.2601E+00
.2578E+00
.2558E+00
.2543E+00
.2531E+00
.2522E+00
.2515E+00
.2510E+00
.2507E+00
.2505E+00
.2502E+00
.2502E+00
.2501E+00
.2501E+00
.2501E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E-1-00
.2500E+00
. 2500E+00
.2500E+00
.2500E+00
.2500E+00
TEMP
(K)
.2772E+03
.2773E+03
.2781E+03
.2787E-K)3
.2793E+03
.2798E-t-03
.2802E+03
.2806E+03
.2809E+03
.2811E+03
.2813E+03
.2814E+03
.2815E+03
.2816E+03
.2816E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
TEMP
(K)
.2772E+03
.2773E+03
.2781E+03
.2787E-K)3
.2793E+03
.2798E-t-03
.2802E+03
.2806E+03
.2809E+03
.2811E+03
.2813E+03
.2814E+03
.2815E+03
.2816E+03
.2816E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
VIRUS
(PFU/ML)
.7458E+05
.6307E+05
.1059E+05
.1096E+04
.7991E+02
.4212E+01
.1636E+00
.4710E-02
.1003E-03
.1572E-05
.1799E-07
.1488E-09
.8829E-12
.3731E-14
.1119E-16
.2380E-19
.3610E-22
.3948E-25
.3167E-28
.1911E-31
.8955E-35
.3381E-38
.1070E-41
.2949E-45
.7331E-49
.1691E-52
.3702E-56
.7820E-60
.1612E-63
.3271E-67
.6564E-71
.1308E-74
.2593E-78
.5122E-82
.1009E-85
.1984E-89
.3893E-93
.7628E-97
.1493-100
.2917-104
.5693-108
.1110-111
.2160-115
.4201-119
.8161-123
.1583-126
.3069-130
RCV

.7458E+00
.6307E+00
.1059E+00
.1096E-01
.7991E-03
.4212E-04
.1636E-05
.4710E-07
.1003E-08
.1572E-10
.1799E-12
.1488E-14
.8829E-17
.3731E-19
.1119E-21
.2380E-24
.3610E-27
.3948E-30
.3167E-33
.1911E-36
.8955E-40
.3381E-43
.1070E-46
.2949E-50
.7331E-54
.1691E-57
.3702E-61
.7820E-65
.1612E-68
.3271E-72
.6564E-76
.1308E-79
.2593E-83
.5122E-87
.1009E-90
.1984E-94
.3893E-98
.7628-102
.1493-105
.2917-109
.5693-113
.1110-116
.2160-120
.4201-124
.8161-128
.1583-131
.3069-135
                                     106

-------
48
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62
63
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65
66
67
68
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70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
.4610E+02
.4710E+02
.4810E+02
.4910E+02
.5010E+02
.5110E+02
.5210E+02
.5310E+02
. 5410E+02
.5510E+02
.5610E+02
.5710E+02
.5810E+02
.5910E+02
.6010E+02
.6110E+02
.6210E+02
.6310E+02
.6410E+02
.6510E+02
.6610E+02
.6710E+02
.6810E+02
.6910E+02
.7010E+02
.7110E+02
.7210E+02
.7310E+02
.7410E+02
.7510E+02
.7610E+02
.7710E+02
.7810E+02
.7910E-I-02
.8010E+02
.8110E+02
.8210E+02
.8310E+02
.8410E+02
. 8510E+02
.8610E+02
.8710E+02
.8810E+02
.8910E+02
.9010E+02
.9110E+02
.9210E+02
.9310E+02
.9410E+02
.9510E+02
.9610E+02
.9710E+02
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
,2500E-K)0
.2500E+00
.2500E+00
.2500E-1-00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E-»-00
.2500E+00
.2500E+00
.2500E+00
.2500E-t-00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E+00
.2500E-t-00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E+00
.2500E-KX)
.2500E+00
.2500E+00
. 2500E+00
. 2500E+00
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-1-03
.2817E+03
.2817E+03
.2817E+03
.2817E-1-03
.2817E+03
.2817E+03
.2817E+03
.2817E-K)3
.2817E-K)3
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-1-03
.2817E+03
.2817E-K)3
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.5940-134
.1149-137
.2219-141
.4281-145
.8252-149
.1589-152
.3056-156
.5873-160
.1127-163
.2162-167
.4141-171
.7924-175
.1515-178
.2893-182
.5521-186
.1053-189
.2005-193
.3814-197
.7250-201
.1377-204
.2613-208
.4954-212
.9383-216
.1776-219
.3357-223
.6343-227
.1197-230
.2258-234
.4255-238
.8011-242
.1507-245
.2833-249 '
.5321-253
.9985-257
.1872-260
.3508-264
.6569-268
.1229-271
.2297-275
.4291-279
.8010-283
.1494-286
.2785-290
.5186-294
.9653-298
.1795-301
.3336-305
.6196-309
.1150-312
.2133-316
.3957-320
.OOOOE+00
.5940-139
.1149-142
.2219-146
.4281-150
.8252-154
.1589-157
.3056-161
.5873-165
.1127-168
.2162-172
.4141-176
.7924-180
.1515-183
.2893-187
.5521-191
.1053-194
.2005-198
.3814-202
.7250-206
.1377-209
.2613-213
.4954-217
.9383-221
.1776-224
.3357-228
.6343-232
.1197-235
.2258-239
.4255-243
.8011-247
.1507-250
.2833-254
.5321-258
.9985-262
.1872-265
.3508-269
.6569-273
.1229-276
.2297-280
.4291-284
.8010-288
.1494-291
.2785-295
.5186-299
.9653-303
.1795-306
.3336-310
.6196-314
.1150-317
.2124-321
.OOOOE+00
.OOOOE+00
107

-------
100       .9810E+02
101       .9910E+02
   TIHEST- .6000E+01
LAYER   NODE POSITION
          ( CM )
  1       .OOOOE+00
  2       .1000E+00
  3       .1100E+01
  4       .2100E+01
  5       .3100E+01
  6       .4100E+01
  7       .5100E+01
  8       .6100E+01
  9       .7100E+01
 10       .8100E+01
 11       .9100E+01
 12       .1010E+02
 13       .1110E+02
 14       .1210E+02
 15       .1310E+02
 16       .1410E+02
 17       .1510E+02
 18       .1610E+02
 19       .1710E+02
 20       .1810E+02
 21       .1910E+02
 22       .2010E+02
 23       .2110E+02
 24       .2210E+02
 25       .2310E+02
 26       .2410E+02
 27       .2510E+02
 28       .2610E+02
 29       .2710E+02
 30       .2810E+02
 31       .2910E+02
 32       .3010E+02
 33       .3110E+02
 34       .3210E+02
 35       .3310E+02
 36       .3410E+02
 37       .3510E+02
 38       .3610E+02
 39       .3710E+02
 40       .3810E+02
 41       .3910E+02
 42       .4010E+02
 43       .4110E+02
 44       .4210E+02
 45       .4310E+02
 46       .4410E+02
 47       .4510E+02
.2SOOE+00
.2500E+00
WATER
(CM3/CM3 SOIL)
.3059E+00
.3056E+00
.3026E+00
.2995E+00
.2964E+00
.2932E+00
.2899E+00
.2867E+00
.2835E+00
.2802E+00
.2771E+00
.2740E+00
.2710E+00
.2682E+00
.2655E+00
.2631E+00
.2609E+00
.2589E+00
.2572E+00
.2558E+00
.2545E+00
.2535E+00
.2527E+00
.2521E+00
.2516E+00
.2512E+00
.2509E+00
.2507E+00
.2505E+00
.2503E+00
.2503E+00
.2502E+00
.2501E+00
.2501E+00
.2501E+00
.2500E+00
.2500E-I-00
.2500E+00
.2500E+00
. 2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E+00
.2500E+00
.2500E+00
.2500E+00
.2817E+03
.2S17E+03
TEMP
(K)
.2763E+03
.2764E+03
.2770E+03
.2776E+03
.2781E+03
.2786E+03
.2791E+03
.2795E+03
.2798E+03
.2802E+03
.2804E+03
.2807E+03
.2809E+03
.2810E+03
.2812E+03
.2813E+03
.2814E+03
.2815E+03
.2815E+03
.2816E+03
.2816E+03
.2816E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-HD3
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.OOOOE+00
. OOOOE+00
VIRUS
(PFU/ML)
.1061E+06
.9794E+05
.3055E+05
.6254E+04
.9466E+03
.1097E+03
.1001E+02
.7306E+00
.4309E-01
.2063E-02
.8033E-04
.2540E-05
.6503E-07
.1342E-08
.2218E-10
.2918E-12
.3034E-14
.2478E-16
.1579E-18
.7813E-21
.2990E-23
.8835E-26
.2015E-28
.3559E-31
.4890E-34
.5274E-37
.4517E-40
.3119E-43
.1768E-46
.8399E-50
.3421E-53
.1222E-56
.3916E-60
.1149E-63
.3144E-67
.8153E-71
.2029E-74
.4893E-78
.1153E-81
.2672E-85
.6113E-89
.1386E-92
.3118E-96
.6981-100
.1557-103
.3460-107
.7669-111
.OOOOE+00
.OOOOE+00
RCV

.1061E+01
.9794E+00
.3055E+00
.6254E-01
.9466E-02
.1097E-02
.1001E-03
.7306E-05
.4309E-06
.2063E-07
.8033E-09
.2540E-10
.6503E-12
.1342E-13
.2218E-15
.2918E-17
.3034E-19
.2478E-21
.1579E-23
.7813E-26
.2990E-28
.8835E-31
.2015E-33
.3559E-36
.4890E-39
.5274E-42
.4517E-45
.3119E-48
.1768E-51
.8399E-55
.3421E-58
.1222E-61
.3916E-65
.1149E-68
.3144E-72
.8153E-76
.2029E-79
.4893E-83
.1153E-86
.2672E-90
.6113E-94
.1386E-97
.3118-101
.6981-105
.1557-108
.3460-112
.7669-116
                                   108

-------
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
.4610E+02
.4710E+02
.4810E+02
.4910E+02
. 5010E+02
.5110E-f02
. 5210E+02
.5310E+02
. 5410E+02
.5510E+02
. 5610E+02
. 5710E+02
.S810E-M32
.5910E-f02
. 6010E+02
.6110E+02
.6210E+02
.6310E+02
.6410E+02
.6510E+02
.6610E+02
.6710E+02
.6810E+02
.6910E+02
.7010E+02
.7110E+02
.7210E+02
.7310E+02
. 7410E+02
.7510E+02
.7610E+02
.7710E+02
.7810E+02
.7910E+02
.8010E-I-02
.8110E+02
.8210E-I-02
.8310E+02
.8410E+02
.8510E+02
.8610E+02
.8710E+02
.8810E+02
.8910E+02
.9010E+02
.9110E+02
.9210E+02
.9310E^02
. 9410E-I-02
.9510E+02
.9610E+02
.9710E+02
.2500E+00
,2500E-fOO
.2500E+00
.2500E+00
.2500E-I-00
.2500E+00
,2500E-fOO
o 2500E-J-00
.2500E-fOO
.2500E+00
. 2500E+00
. 2SOOE+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E+00
.2500E+00
. 2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E-fOO
.2500E-»-00
.2500E-I-00
.2500E-fOO
.2500E+00
. 2500E+00
.2500E+00
. 2500E+00
.2500E+00
.2500E-K)0
.2500E+00
.2500E+00
. 2500E+00
. 2500E+00
.2817E+03
.2817E+03
.2817E+03
.2817E-4-03
.2817E+03
.2817E+03
.2817E+03
.2817E-K)3
.2817E+03
.2817E+03
.2S17E+03
.2817E+03
.2817E-f03
.2817E-K)3
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-I-03
.2817E+03
.2817E+03
.2817E-»-03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-K)3
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.1696-114
.3745-118
.8255-122
.1817-125
.3992-129
.8760-133
.1920-136
.4202-140
.9185-144
.2005-147
.4372-151
.9520-155
.2071-158
.4498-162
.9759-166
.2115-169
.4577-173
.9895-177
.2136-180
.4608-184
.9925-188
.2136-191
.4589-195
.9851-199
.2112-202
.4524-206
.9677-210
.2068-213
.4413-217
.9408-221
.2003-224
.4261-228
.9054-232
.1922-235
.4074-239
.8628-243
.1825-246
.3857-250
.8142-254
.1717-257
.3616-261
.7609-265
.1599-268
.3358-272
.7045-276
.1476-279
.3090-283
.6461-287
.1350-290
.2817-294
.5873-298
.1223-301
.1696-119
.3745-123
.8255-127
.1817-130
.3992-134
.8760-138
.1920-141
.4202-145
.9185-149
.2005-152
.4372-156
.9520-160
.2071-163
.4498-167
.9759-171
.2115-174
.4577-178
.9895-182
.2136-185
.4608-189
.9925-193
.2136-196
.4589-200
.9851-204
.2112-207
.4524-211
.9677-215
.2068-218
.4413-222
.9408-226
.2003-229
.4261-233
.9054-237
.1922-240
.4074-244
.8628-248
.1825-251
.3857-255
.8142-259
.1717-262
.3616-266
.7609-270
.1599-273
.3358-277
.7045-281
.1476-284
.3090-288
.6461-292
.1350-295
.2817-299
.5873-303
.1223-306
109

-------
100       .9810E+02     .2500E+00     .2817E+03      .2545-305      .2545-310
101       .9910E+02     .2500E+00     .2817E+03      .1435-305      .1435-310
                                  110

-------
Output Data File: P2RNFL

  TIME    WATER EVAP.    TEMPI    TEMP5    TEMP10    TEMP20    TEMP50
   .1000E+01-.6399E-03  .2784E+03  .2814E+03 .2817E+03 .2817E+03 .2817E+03
   .2000E+01-.3555E-03  .2777E+03  .2808E+03 .2816E+03 .2817E+03 .2817E+03
   .3000E+01-.2015E-03  .2772E+03  .2802E-1-03 .2814E+03 .2817E+03 .2817E+03
   .4000E+01-.9010E-04  .2769E+03  .2798E+03 .2812E+03 .2817E-I-03 .2817E+03
   .5000E+01-.1567E-04  .2766E+03  .2794E+03 .2809E+03 .2817E+03 .2817E+03
   .6000E+01  .3717E-04  .2763E+03  .2791E+03 .2807E+03 .2816E+03 .2817E+03
                                       111

-------
Output Data File: TEST.OUT

                    RUN CONTROL INFORMATION.
          T0«     .0000  TOUT-    6.1000  DTO-     .1000

          PRTIN(I)-     1.0000     2.9900     5.9900    17.9900    23.9900

    29.9900    35.9900    41.9900    47.9900    71.9900

              119.9900   139.9900   149.9900   159.9900


                  000000101010000010
   169.9900
NPRINT(I)-  0
NNSTR2(I)«  1  2  3  4  5  6  7   8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
24 25

          LAYER, THICKNESS(CM),  NODAL POSITION(CM).
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
. 1000E+00
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
. lOOOE-f-01
.1000E+01
.1000E+01
. 1000E+01
. 1000E+01
. lOOOE-f 01
. 1000E+01
. 1000E+01
.1000E+01
. 1000E+01
.1000E+01
.OOOOE+00
. 1000E+00
.1100E+01
.2100E+01
.3100E+01
.4100E+01
.5100E+01
.6100E+01
.7100E+01
.8100E+01
.9100E+01
.1010E+02
.1110E+02
.1210E+02
.1310E+02
.1410E+02
.1510E+02
.1610E+02
.1710E+02
.1810E+02
.1910E+02
.2010E+02
.2110E+02
.2210E+02
.2310E+02
.2410E+02
.2510E-t-02
.2610E-J-02
.2710E-V02
.2810E+02
.2910E+02
.3010E+02
.3110E+02
                                      112

-------
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
. 1000E+01
.1000E+01
.1000E+01
. 1000E+01
. 1000E+01
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
. 1000E+01
.1000E+01
. 1000E+01
. 1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
.1000E+01
.1000E+01
. 1000E+01
.1000E+01
.1000E+01
. 1000E-I-01
.1000E+01
. 1000E+01
. 1000E+01
.1000E+01
. 1000E+01
. 1000E+01
.1000E-K)!
. 1000E+01
. 1000E+01
.lOOOE-t-01
.1000E+01
. 1000E+01
.1000E+01
. 1000E-K)!
. 1000EH-01
. 1000E+01
.1000E+01
.3210E+02
.3310E+02
.3410E+02
.3510E+02
.3610E+02
.3710E-I-02
.3810E+02
.3910E+02
.4010E+02
.4110E+02
.4210E-K)2
.4310E+02
.4410E+02
.4510E+02
.4610E+02
.4710E+02
.4810E+02
.4910E+02
.5010E+02
.5110E+02
.5210E+02
.5310E+02
.5410E+02
.5510E+02
.5610E+02
.5710E-t-02
.5810E+02
.5910E+02
.6010E+02
.6110E+02
.6210E+02
.6310E+02
.6410E+02
.6510E+02
.6610E+02
.6710E+02
.6810E+02
.6910E+02
.7010E+02
.7110E-I-02
.7210E+02
.7310E+02
.7410E+02
.7510E+02
.7610E+02
.7710E+02
.7810E+02
.7910E+02
.8010E+02
.8110E+02
.8210E+02
.8310E+02
                          113

-------
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
.1000E+01
.1000E+01
.1000E+01
. 1000E+01
. 1000E+01
. 1000E+01
. 1000E+01
. 1000E+01
. 1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.1000E+01
.8410E+02
.8510E+02
.8610E+02
.8710E+02
.8810E-I-02
.8910E-I-02
.9010E+02
.9110E+02
.9210E-I-02
.9310E+02
.9410E+02
.9510E+02
.9610E+02
.9710E+02
.9810E+02
NUMBER OF INTERNAL SOIL NODES-  99   SOIL THICKNESS (CM)-

      BOUNDARY LAYER THICKNESS-    1.0000
                                    .2640E+01  RHOSIL-

                                    .7000E-01  ALBSOI-

                                    .9bOOE+00  EMSSOI-
RHOSND-      .2660E+01  RHOCLA-

ALBAIR-      .5000E-01  ALBWAT-

EMSAIR-      .9000E+00  EMSWAT-

LAMBHT-      .3330E-03

LAMSLD -     .3430E+01
 SHTSAN-     .1750E+00  SHTSIL-     .1750E+00  SHTCLA-
  . lOOOE-t-01  SHTAIR-     . 2400E+00
   99.1000



.2650E+01

 .9000E-01

 .5000E+00
                                                          .1750E+00  SHTWAT-
 GAMTLI-    -.2090E-02  BETATV-     .1056E-05

 DWVAR(I)-      .9540E+03     .4770E+04     .3330E-03

 RHOWAT-    .1000E+01  RHOAIR-    .1100E-02  WS  -     .OOOOE+00


           DRIVING FUNCTION PARAMETERS.


   FOURIER COEFFICIENTS

 NCFTMP -  2   NCFFRH -  5
 INDEX   ATEMP
   1   -1.006722
                     BTEMP
                  -2.356627
                                      114

-------
       -.371836
1.000000
INDEX
1
2
3
4
5
ARHIN
.037193
-.005292
-.009458
.006375
-.006108
BRH1N
.067587
-.027424
.011667
-.004330
.004079
TPINMU -   8.757800   RHINMU -
               .869000   TPWATI - 277.000000
INDEX
1
2
INDEX
1
2
3
4
5
INDEX
1
2
3
4
5
INDEX
1
2
3
4
5
INDEX
1
2
OMEGTP
.261199
.523598
OMGRHI
.261199
.523598
.785397
1.047196
1.308995
QRAIN QSR
.OOOOE+00
.1000E+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
TIME LIGHT ON
.6000E+01
. 3000E+02
. 5400E+02
.7800E+02
.1020E+03
TIME RAIN ON
. OOOOE+00
.1300E+03










OOOOE+00
3561E+02
6639E+01
2510E+01
1500E+00
TIME LIGHT OFF
.1800E+02
.4200E+02
.6600E+02
.9000E+02
.1140E+03
TIME RAIN OFF
.6000E+01
. 1400E+03
         VIRUS  PARAMETERS
                                     115

-------
    DISPLZ-  .1000E+01  DLO-  .3240E-03  CWIN-  .1000E+06  CVGRD-  .OOOOE+00
  AIR R.H.
 (PERCENT)
   AIR TEMP-
(DEC.  KELVIN)
     .8920E+00
         .2817E-4-03
    INITIAL FIELD DISTRIBUTIONS

LAYER          WATER
       (CM3 WATER/CM-3 SOIL)
   1          .2500E+00
   2          .2500E+00
   3          .2500E+00
   4          .2500E+00
   5          .2500E+00
   6          .2500E+00
   7          .2500E+00
   8          .2500E+00
   9          .2500E+00
  10          .2500E+00
  11          .2500E+00
  12          .2500E+00
  13          .2500E+00
  14          .2500E+00
  15          .2500E+00
  16          .2500E+00
  17          .2500E+00
  18          .2500E+00
  19          .2500E+00
  20          .2500E+00
  21          .2500E+00
  22          .2500E+00
  23          .2500E+00
  24          .2500E+00
  25          .2500E+00
  26          .2500E+00
  27          .2500E+00
  28          .2500E+00
  29          .2500E-I-00
  30          .2500E+00
  31          .2500E+00
  32          .2500E+00
  33          .2500E+00
  34          .2500E+00
  35          .2500E+00
  36          .2500E+00
  37          .2500E-I-00
  38          .2500E-K)0
  39          .2500E+00
  40          .2500E+00
                      TEMPERATURE
                      (DEC.  KELVIN)
                       .2817E+03
                       .2817E+03
                       .2817E-I-03
                       .2817E+03
                       .2817E+03
                       .2817E-I-03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E-K)3
                       .2817E+03
                       .2817E-I-03
                       .2817E-K)3
                       .2817E+03
                       .2817E+03
                       .2817E+03
  VIRUS
(PFU/ML)
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
                                     116

-------
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
.2500E+00
.2500E+00
.2500E4-00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
. 2500E-I-00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E-I-00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-H03
.2817E+03
.2817E-K)3
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-f03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-I-03
.2817E+03
.2817E+03
.2817E+03
.2817E-J-03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E-KJ3
.2817E+03
.2817E+03
.OOOOE+00
.OOOOE+00
.OOOOE-l-00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
                                   117

-------
  93
  94
  95
  96
  97
  98
  99
 100
 101
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2500E+00
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
     UNIVERSAL CONSTANTS

GRAV -     .1271E+11  R -     .5981E+14  SIGMA

         TABLES OF SOIL PROPERTIES
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
.OOOOE+00
                                       .4896E-08
         THTAST(I)
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
. 5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
. 5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
. 5500E+00

.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00

.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
.5500E+00
. 5500E+00
.5500E+00
.5500E+00

TORT(I)
.6600E+00
6600E+00
.6600E+00
6600E+00
.6600E+00
6600E+00
.6600E+00
6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00

                                                       .5500E+00
                                                       .6600E+00

                                                       .6600E+00

                                                       .6600E+00

                                                       .6600E+00

                                                       .6600E+00
                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00

                                                      .5500E+00
                                                      .6600E+00

                                                      .6600E+00

                                                      .6600E+00

                                                      .6600E+00

                                                      .6600E+00
                                     118

-------
6600E+00
.6600E+00
6600E+00
.6600E+00
6600E+00
. 6600E+00
6600E+00
.6600E+00
6600E+00
.6600E+00
6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00

.6600E+00
.6600E+00
. 6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E-f-00
.6600E+00

.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
.6600E+00
. 6600E+00
.6600E+00


.6600E+00

.6600E+00

.6600E+00

. 6600E+00

.6600E+00



.6600E+00

.6600E+00

.6600E+00

.6600E+00

.6600E+00


        EPS(I)
   .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  . 5500E-KOO
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
   .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E-I-00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
.5500E+00
  .5500E+00
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        PCTSAN(I)
   .3000E-I-00
.3000E+00
  .3000E+00
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5500E+00
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5500E+00
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5500E+00
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                                   119

-------
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PCTCIA(I)
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PCTSIL(I)
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120

-------
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        RHOSOL(I)
   .2652E-K)!
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        RHOB(I)
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                                   121

-------
        SHTSOL(I)
   .1750E+00
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        LAMSOL(I)
   .3A30E+01
.3430E+01
  .3430E+01
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        ALPTH(I)
   .1241E+04
.1241E-M34
   .1241E+04
.1241E+04
   .1241E+04    .1241E+04
.1241E+04     .1241E+04
.1241E+04
.1241E+04
                                   122

-------
- 1241E+04
1241E+04
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BETATH(I)
.7079E+00
7079E+00
.7079E+00
7079E+00
.7079E+00
7079E+00
.7079E+00
7079E+00
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.7079E+00
.7079E+00
.7079E+00

GAMCNS(I)
.6500E+01
6500E+01
.6500E+01
6500E+01
.6500E+01
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.7079E+00




.7079E+00




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123

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.6500E-K)!
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  .6500E+01
.6500E+01
  .6500E+01
.6500E+01
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        KTHSTS(I)
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  .6100E+00
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  .6100E+00
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  .6500E+01
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  .6500E-t-01
.6500E+01
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. 6500E-I-01
  .6500E+01
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.6500E+01

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   .2900E-01
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eiooE-t-oo
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6100E+00
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6100E+00
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6100E+00
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6100E+00
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.2900E-01
                                   124

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2900E-01
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2900E-01
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2900E-01
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.2900E-01

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.2900E-01


        DALPTZ(I)
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                                   125

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        DTHTSZ(I)
   .OOOOE+00
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                                   126

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      OUTPUT DATA FOR DYNAMIC SYSTEM

*** WATER, TEMP.,  AND VIRUS CONC. , RESPECTIVELY, AT TIME T -1.0000 ***
  (DTLAST-       .1000)
 LAYER        WATER
          (CH3/CM3 SOIL)
    1         .2780E+00
    2         .2774E+00
    3         .2717E+00
    4         .2663E+00
    5         .2616E+00
    6         .2578E+00
    7         .2550E+00
    8         .2531E+00
    9         .2518E+00
   10         .2510E+00
   11         .2506E+00
   12         .2503E+00
   13         .2502E+00
   14         .2501E+00
   15         .2500E+00
   16         .2500E+00
   17         .2500E+00
   18         .2500E+00
   19         .2500E+00
   20         .2500E+00
   21         .2500E+00
   22         .2500E+00
   23         .2500E+00
   24         .2500E+00
   25         .2500E+00
              FIELD DISTRIBUTIONS

                     TEMPERATURE
                       (DEC. KELVIN)
                       .2784E+03
                       .2785E+03
                       .2795E+03
                       .2803E+03
                       .2808E+03
                       .2812E+03
                       .2814E+03
                       .2816E+03
                       .2816E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                       .2817E+03
                               VIRUS
                            (PFU/ML)
                              .4090E+05
                              .2580E+05
                              .1508E+04
                              .4823E+02
                              .9113E+00
                              .1024E-01
                              .6823E-04
                              .2687E-06
                              .6259E-09
                              .8731E-12
                              .7494E-15
                              .4150E-18
                              .1588E-21
                              .4559E-25
                              .1070E-28
                              .2203E-32
                              .4196E-36
                              .7646E-40
                              .1360E-43
                              .2386E-47
                              .4159E-51
                              .7219E-55
                              .1250E-58
                              .2162E-62
                              .3734E-66
NODE
PS I
KONTHS
VLZZ
1
2
3
4
5
6
7
8
9
10
11
-.8527E+03
-.8563E+03
-.8905E+03
-.9246E+03
- . 9554E+03
-.9811E+03
- . 1001E+04
- . 1014E+04
-.1023E+04
- . 1029E+04
- . 1032E+04
.5014E-02
.4935E-02
.4254E-02
.3677E-02
.3230E-02
.2903E-02
.2679E-02
.2532E-02
.2441E-02
.2387E-02
.2355E-02
.1873E+00
.1833E+00
.1495E+00
.1230E+00
.9445E-01
.6859E-01
.4730E-01
.3136E-01
.2029E-01
.1305E-01
.8550E-02
                                     127

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12
13
14
15
16
17
18
19
20
21
22
23
24
25
-.1034E+04
- . 1035E+04
-.1036E+04
-.1036E+04
- . 1036E+04
-.1037E+04
- . 1037E+04
-.1037E-K>4
- . 1037E+04
- . 1037E+04
-.1037E+04
-.1037E+04
-.1037E+04
-.1037E+04
.2336E-02
.2326E-02
.2321E-02
.2318E-02
.2316E-02
.2315E-02
.2315E-02
.2315E-02
.2314E-02
.2314E-02
.2314E-02
.2314E-02
.2314E-02
.2314E-02
                                    .5848E-02
                                    .4275E-02
                                    .3382E-02
                                    .2886E-02
                                    .2616E-02
                                    .2472E-02
                                    .2395E-02
                                    .2355E-02
                                    .2335E-02
                                    .2325E-02
                                    .2319E-02
                                    .2317E-02
                                    .2316E-02
                                    .2315E-02
          IEVENT(1)- 0
 IEVENT(2)-
     RELATIVE HUMIDITY OF THE OUTER BOUNDARY LAYER AIR- .1000E+01
     RELATIVE HUMIDITY AT THE SOIL SURFACE- .9993E+00
     SATURATED WATER VAPOR DENSITY IN AIR- .6124E-05
     SATURATED WATER VAPOR DENSITY AT AIR-SOIL INTERFACE- .6857E-05
     EVAPORATIVE(-) OR CONDENSIVE(+) FLUX--.6399E-03
     AIR TEMP. AT OUTER BOUNDARY LAYER EDGE- .2767E+03
     SURFACE INFILTRATION RATE(CM/HR)- .1000E+00
     TEMP, PAIN WATER- .2770E+03
     SOLAR RADIATION- .OOOOE+00
     EVAPORATIVE(-) OR CONDENSIVE(+) HEAT TRANSFER—.3794E+00
     SENSIBLE HEAT TRANSFER--.3552E+00
     TRANSFER OF HEAT OF RAIN WATER INTO SOIL- .4000E+00
*** WATER, TEMP., AND VIRUS CONC.,  RESPECTIVELY,  AT TIME T - 2.0000***
  (DTLAST-      .1000)
 LAYER        WATER
          (CM3/CM3 SOIL)
    1        .2871E+00
    2        .2866E+00
    3        .2822E+00
    4        .2776E+00
    5        .2732E+00
    6        .2689E+00
    7        .2650E+00
    8        .2615E+00
    9        .2586E+00
   10        .2562E+00
   11        .2543E+00
   12        .2529E+00
   13        .2520E+00
   14        .2513E+00
   15        .2508E+00
   16        .2505E+00
FIELD DISTRIBUTIONS

       TEMPERATURE
        (DEC. KELVIN)
        .2777E+03
        .2778E+03
        .2786E+03
        .2793E+03
        .2799E+03
        .2804E+03
        .2808E+03
        .2811E+03
        .2813E+03
        .2814E+03
        .2815E-I-03
        .2816E+03
        .2817E-f03
        .2817E+03
        .2817E+03
        .2817E+03
   VIRUS
(PFU/ML)
  .5940E+05
  .4629E+05
  .5336E+04
  .3677E+03
  .1700E+02
  .5377E+00
  .1175E-01
  .1768E-03
  .1818E-05
  .1266E-07
  .5921E-10
  .1850E-12
  .3862E-15
  .5429E-18
  .5222E-21
  .3530E-24
                                      128

-------
   17
   18
   19
   20
   21
   22
   23
   24
   25

NODE

   1
   2
   3
   4
   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
  16
  17
  18
  19
  20
  21
  22
  23
  24
  25
     .2503E+00
     .2502E+00
     .2501E+00
     .2501E+00
     .2500E+00
     .2500E+00
     .2500E+00
     .2500E+00
     .2500E+00
   PS I

.8001E+03
.8029E+03
.8276E+03
.8544E+03
.8813E-I-03
.9079E+03
.9331E+03
.9561E+03
.9761E+03
.9927E+03
.1006E+04
.1015E+04
.1022E+04
.1027E+04
.1031E+04
.1033E+04
.1034E+04
.1035E+04
.1036E+04
.1036E+04
.1036E+04
.1036E+04
.1037E+04
.1037E+04
.1037E+04

 lEVENT(l)- 0
KONTHS

.6333E-02
.6255E-02
.5601E-02
.4977E-02
.4426E-02
.3949E-02
.3547E-02
.3220E-02
.2963E-02
.2768E-02
.2625E-02
.2522E-02
.2451E-02
.2403E-02
.2371E-02
.2350E-02
.2336E-02
.2328E-02
.2322E-02
.2319E-02
.2317E-02
.2316E-02
.2315E-02
.2315E-02
.2315E-02
.2817E+03
.2817E-I-03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03

    VLZZ

  .1826E+00
  .1786E-J-00
  .1499E+00
  .1387E+00
  .1228E+00
  .1062E+00
  .8912E-01
  .7247E-01
  .5707E-01
  .4363E-01
  .3249E-01
  .2372E-01
  .1710E-01
  .1230E-01
  .8914E-02
  .6599E-02
  .5050E-02
  .4035E-02
  -3381E-02
  .2967E-02
  .2709E-02
  .2550E-02
  .2454E-02
  .2396E-02
  .2361E-02
                              .1742E-27
                              .6576E-31
                              .2003E-34
                              .5187E-38
                              .1196E-41
                              .2550E-45
                              .5164E-49
                              .1012E-52
                              .1943E-56
                          IEVENT(2)-
    RELATIVE HUMIDITY OF THE OUTER BOUNDARY LAYER AIR- .1000E+01
    RELATIVE HUMIDITY AT THE SOIL SURFACE- .9994E+00
    SATURATED WATER VAPOR DENSITY IN AIR- .6131E-05
    SATURATED WATER VAPOR DENSITY AT AIR-SOIL INTERFACE- .6528E-05
    EVAPORATIVE(-) OR CONDENSIVE(+) FLUX—.3555E-03
    AIR TEMP. AT OUTER BOUNDARY LAYER EDGE- .2767E+03
    SURFACE INFILTRATION RATE(CM/HR)- .1000E-*-00
    TEMP. RAIN WATER- .2770E+03
    SOLAR RADIATION- .OOOOE+00
    EVAPORATIVE(-) OR CONDENSIVE(+) HEAT TRANSFER— . 2109E+00
    SENSIBLE HEAT TRANSFER-- .1974E+00
    TRANSFER OF HEAT OF RAIN WATER INTO SOIL- .4000E+00
                                    129

-------
*** WATER, TEMP. ,  AND VIRUS CONC. , RESPECTIVELY, AT TIME T - 3.0000***
    .1000)
                                                            (DTLAST-
 LAYER

    1
    2
    3
    4
    5
    6
    7
    8
    9
   10
   11
   12
   13
   14
   15
   16
   17
   18
   19
   20
   21
   22
   23
   24
   25

 NODE
    WATER
(CM3/CM3 SOIL)
   .2931E+00
   .2926E+00
   .2889E+00
   .2849E+00
   .2809E+00
   .2770E+00
   .2731E+00
   .2694E+00
   .2660E+00
   .2629E+00
   .2601E+00
   .2578E+00
   .2558E+00
   .2543E+00
   .2531E+00
   .2522E+00
   .2515E+00
   .2510E+00
   .2507E+00
   .2505E+00
   .2503E+00
   .2502E+00
   .2501E+00
   .2501E+00
   .2501E+00
    FIELD DISTRIBUTIONS

           TEMPERATURE
            (DEC. KELVIN)
            .2772E+03
            .2773E+03
            .2781E+03
            .2787E+03
            .2793E+03
            .2798E+03
            .2802E+03
            .2806E+03
            .2809E+03
            .2811E+03
            .2813E+03
            .2814E+03
            .2815E+03
            .2816E+03
            .2816E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
            .2817E+03
  PSI
KONTHS
VLZZ
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
-.7673E+03
--7697E+03
-.7900E+03
-.8124E+03
-.8352E+03
-.8585E+03
-.8818E+03
- . 9047E+03
-.9267E-KJ3
-.9471E+03
-.9655E+03
-.9815E-I-03
-.9950E+03
-.1006E-K)4
- . 1014E+04
-.1021E+04
-.1026E+04
.7348E-02
.7269E-02
.6628E-02
.5994E-02
.5416E-02
.4889E-02
.4418E-02
.4003E-02
.3646E-02
.3345E-02
.3097E-02
.2898E-02
.2742E-02
.2623E-02
.2533E-02
.2468E-02
.2420E-02
.1798E+00
.1758E+00
.1481E+00
. 1414E+00
.1302E+00
.1188E+00
.1066E+00
.9383E-01
.8095E-01
.6834E-01
.5641E-01
.4556E-01
.3605E-01
.2803E-01
.2150E-01
.1635E-01
.1240E-01
              VIRUS
           (PFU/ML)
             .7458E+05
             .6307E+05
             .1059E+05
             .1096E+04
             .7991E+02
             .4212E+01
             .1636E+00
             .4710E-02
             .1003E-03
             .1572E-05
             .1799E-07
             .1488E-09
             .8829E-12
             .3731E-14
             .1119E-16
             .2380E-19
             .3610E-22
             .3948E-25
             .3167E-28
             .1911E-31
             .8955E-35
             .3381E-38
             .1070E-41
             .2949E-45
             .7331E-49
                                     130

-------
   18   -.1029E+04     .2387E-02     .9440E-02
   19   -.1031E+04     .2363E-02     .7276E-02
   20   -.1033E+04     .2347E-02     .5722E-02
   21   -.1034E+04     .2336E-02     .4626E-02
   22   -.1035E+04     .2328E-02     .3863E-02
   23   -.1036E+04     .2323E-02     .3341E-02
   24   -.1036E+04     .2320E-02     .2987E-02
   25   -.1036E+04     .2318E-02     .2751E-02

          lEVENT(l)- 0     1EVENT(2)- 1

     RELATIVE HUMIDITY OF THE OUTER BOUNDARY LAYER AIR- .1000E+01
     RELATIVE HUMIDITY AT THE SOIL SURFACE- .9994E+00
     SATURATED WATER VAPOR DENSITY IN AIR- .6131E-05
     SATURATED WATER VAPOR DENSITY AT AIR-SOIL INTERFACE- .6355E-05
     EVAPORATIVE(-) OR CONDENSIVE(-l-) FLUX--.2015E-03
     AIR TEMP. AT OUTER BOUNDARY LAYER EDGE- .2767E+03
     SURFACE INFILTRATION RATE(CM/HR)- .1000E+00
     TEMP. RAIN WATER- .2770E+03
     SOLAR RADIATION-  .OOOOE+00
     EVAPORATIVE(-) OR CONDENSIVE(+) HEAT TRANSFER—.1196E+00
     SENSIBLE HEAT TRANSFER--.1126E+00
     TRANSFER OF HEAT OF RAIN WATER INTO SOIL- .4000E+00
*** WATER, TEMP., AND VIRUS CONC., RESPECTIVELY, AT TIME T - 4.0000***  (DTLAST-
    .1000)
                                      131

-------
                         FIELD DISTRIBUTIONS
LAYER

   1
   2
   3
   4
   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
  16
  17
  18
  19
  20
  21
  22
  23
  24
  25

NODE

   1
   2
   3
   4
   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
  16
  17
  18
  19
  20
     WATER
 (CM3/CM3 SOIL)
    .2980E+00
    .2976E+00
    .2942E+00
    .2906E+00
    .2870E+00
    .2833E+00
    .2797E+00
    .2761E+00
    .2726E+00
    .2693E+00
    .2662E+00
    .2633E+00
    .2608E+00
    .2586E+00
    .2567E+00
    .2551E+00
    .2539E+00
    .2529E+00
    .2521E+00
    .2515E+00
    .2511E+00
    .2508E+00
    .2505E+00
    .2504E+00
    .2503E+00
   PSI

.7410E+03
.7430E-I-03
.7608E+03
.7804E+03
.8005E+03
.8212E-1-03
.8424E+03
.8637E+03
.8849E-H33
.9057E+03
. 9255E+03
.9442E+03
.9611E+03
.9762E+03
.9892E+03
, 1000E+04
.1009E-KJ4
. 1016E+04
.1022E+04
.1026E+04
KONTHS

.8296E-02
.8218E-02
.7570E-02
.6922E-02
.6321E-02
.5762E-02
.5247E-02
.4778E-02
.4358E-02
.3987E-02
.3663E-02
.3386E-02
.3154E-02
.2963E-02
.2808E-02
.2685E-02
.2589E-02
.2516E-02
.2460E-02
.2419E-02
TEMPERATURE
(DEC. KELVIN)
.2769E+03
.2770E+03
.2777E-»-03
.2783E+03
.278-9E+03
.2794E+03
.2798E+03
.2802E+03
.2805E+03
.2808E+03
.2810E+03
.2812E-f03
.2813E+03
.2814E+03
.2815E+03
.2816E+03
.2816E+03
.2816E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
.2817E+03
VLZZ
.1775E+00
.1740E+00
.1493E+00
.1443E+00
.1353E+00
.1263E-t-00
.1166E-1-00
. 1064E+00
.9578E-01
.8496E-01
.7416E-01
.6365E-01
.5370E-01
.4453E-01
.3634E-01
.2923E-01
.2323E-01
.1831E-01
.1435E-01
.1125E-01
VIRUS
(PFU/ML)
.8707E+05
.7689E+05
.1678E+05
.2310E+04
.2290E+03
.1687E+02
.9463E+00
.4087E-01
.1364E-02
.3514E-04
.6964E-06
.1054E-07
.1210E-09
.1044E-11
.6718E-14
.3206E-16
.1129E-18
.2930E-21
.5609E-24
.7967E-27
.8481E-30
.6870E-33
.4323E-36
.2167E-39
.8914E-43





















                                     132

-------
   21   -.1029E+04
   22   -.1031E+04
   23   -.1033E+04
   24   - . 1034E+04
   25   -.1035E+04

          lEVENT(l)-  0
.2389E-02
.2367E-02
.2351E-02
.2340E-02
.2332E-02
.8861E-02
.7053E-02
.5707E-02
.4717E-02
.4000E-02
     IEVENT(2)-
     RELATIVE HUMIDITY OF THE OUTER BOUNDARY LAYER AIR-  .1000E+01
     RELATIVE HUMIDITY AT THE SOIL SURFACE- .9994E+00
     SATURATED WATER VAPOR DENSITY IN AIR- .6111E-05
     SATURATED WATER VAPOR DENSITY AT AIR-SOIL INTERFACE-  .6213E-05
     EVAPORATIVE(-) OR CONDENSIVE(+) FLUX—.9010E-04
     AIR TEMP. AT OUTER BOUNDARY LAYER EDGE- .2766E+03
     SURFACE INFILTRATION RATE(CM/HR)- .1000E+00
     TEMP. RAIN WATER- .2770E+03
     SOLAR RADIATION- .OOOOE+00
     EVAPORATIVE(-) OR CONDENSIVE(+) HEAT TRANSFER—.5350E-01
     SENSIBLE HEAT TRANSFER—.5151E-01
     TRANSFER OF HEAT OF RAIN WATER INTO SOIL- .4000E+00
*** WATER, TEMP., AND VIRUS  CONC. , RESPECTIVELY, AT TIME T - 5.0000***
  (DTLAST-      .1000)
 LAYER        WATER
          (CM3/CM3 SOIL)
    1         .3022E+00
    2         .3019E+00
    3         .2987E+00
    4         .2954E-t-00
    5         .2920E+00
    6         .2886E+00
    7         .2852E+00
    8         .2818E+00
    9         .2784E+00
   10         .2751E+00
   11         .2719E+00
   12         .2688E+00
   13         .2660E+00
   14         .2633E+00
   15         .2610E+00
   16         .2589E-I-00
   17         .2571E+00
   18         .2556E+00
   19         .2543E+00
   20         .2533E-I-00
   21         .2525E+00
   22         .2519E+00
   23         .2514E-I-00
   24         .2510E+00
   25         .2507E+00
    FIELD DISTRIBUTIONS

           TEMPERATURE
            (DEC.  KELVIN)
            .2766E+03
            .2767E+03
            .2773E+03
            .2779E+03
            .2785E+03
            .2790E+03
            .2794E+03
            .2798E+03
            .2802E+03
            .2804E+03
            .2807E+03
            .2809E+03
            .2811E+03
            .2812E-1-03
            .2814E-K03
            .2814E+03
            .2815E-I-03
            .2816E-K)3
            .2816E-t-03
            .2816E-K)3
            .2817E-K)3
            .2B17E+03
            .2817E-I-03
            .2817E+03
            .2817E+03
                VIRUS
              (PFU/ML)
                .9741E+05
                .8834E+05
                .2353E+05
                .4036E+04
                .5056E+03
                .4790E+02
                .3525E+01
                .2043E+00
                .9399E-02
                .3440E-03
                .1001E-04
                .2306E-06
                .4185E-08
                .5946E-10
                .6563E-12
                .5587E-14
                .3642E-16
                .1808E-18
                .6808E-21
                .1940E-23
                .4183E-26
                .6854E-29
                .8589E-32
                .8321E-35
                .6327E-38
                                      133

-------
NODE
PSI
KONTHS
VLZZ
   1   -.7193E+03
   2   -.7211E+03
   3   -.7371E+03
   4   -.7546E+03
   5   -.7726E+03
   6   -.7913E+03
   7   -.8105E+03
   8   -.8301E+03
   9   -.8499E+03
  10   -.8698E+03
  11   -.8895E+03
  12   -.9086E-K)3
  13   -.9270E+03
  14   -.9442E+03
  15   -.9599E+03
  16   -.9740E+03
  17   -.9864E+03
  18   -.9969E+03
  19   -.1006E+04
  20   -.1013E+04
  21   -.1019E+04
  22   -.1023E+04
  23   -.1027E+04
  24   -.1029E+04
  25   -.1031E+04

         lEVENT(l)- 0
          .9178E-02
          .9101E-02
          .8446E-02
          .7790E-02
          .7172E-02
          .6590E-02
          .6045E-02
          .5540E-02
          .5075E-02
          .4653E-02
          .4273E-02
          .3936E-02
          .3641E-02
          .3386E-02
          .3170E-02
          .2989E-02
          .2841E-02
          .2720E-02
          .2624E-02
          .2548E-02
          .2489E-02
          .2444E-02
          .2409E-02
          .2384E-02
          .2364E-02
              .1758E+00
              .1725E+00
              .1501E+00
              .1462E+00
              .1387E+00
              .1313E+00
              .1233E+00
              .1148E+00
              .1059E+00
              .9667E-01
              .8722E-01
              .7769E-01
              .6828E-01
              .5917E-01
              .5054E-01
              .4256E-01
              .3537E-01
              .2904E-01
              .2360E-01
              .1903E-01
              .1527E-01
              .1223E-01
              .9824E-02
              .7939E-02
              .6485E-02
               IEVENT(2)-
   RELATIVE HUMIDITY OF THE OUTER BOUNDARY LAYER AIR- .1000E+01
   RELATIVE HUMIDITY AT THE SOIL SURFACE- .9994E+00
   SATURATED WATER VAPOR DENSITY IN AIR- .6069E-05
   SATURATED WATER VAPOR DENSITY AT AIR-SOIL INTERFACE- .6091E-05
   EVAPORATIVE(-) OR CONDENSIVE(+) FLUX—.1567E-04
   AIR TEMP. AT OUTER BOUNDARY LAYER EDGE- .2765E+03
   SURFACE INFILTRATION RATE(CM/HR)- .lOOOE-MDO
   TEMP- RAIN WATER-  .2770E+03
   SOLAR RADIATION- .OOOOE+00
   EVAPORATIVE(-) OR CONDENSIVE(-l-) HEAT TRANSFER—.9309E-02
   SENSIBLE HEAT TRANSFER—.1051E-01
   TRANSFER OF HEAT OF RAIN WATER INTO SOIL- .4000E+00
   WATER, TEMP., AND VIRUS CONC., RESPECTIVELY, AT TIME T -6.0000 ***
(DTLAST-      .1000)
LAYER        WATER
         (CM3/CM3  SOIL)
   1         .3059E+00
   2         .3056E-I-00
   3         .3026E+00
              FIELD DISTRIBUTIONS

                     TEMPERATURE
                      (DEC. KELVIN)
                      .2763E+03
                      .2764E+03
                      .2770E+03
                               VIRUS
                            (PFU/ML)
                              .1061E+06
                              .9794E+05
                              .3055E+05
                                     134

-------
   4
   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
  16
  17
  18
  19
  20
  21
  22
  23
  24
  25

NODE
  .2995E+00
  .2964E+00
  .2932E+00
  .2899E+00
  .2867E+00
  .2835E+00
  .2802E+00
  .2771E-I-00
  .2740E+00
  .2710E-K)0
  .2682E+00
  .2655E+00
  .2631E+00
  .2609E+00
  .2589E+00
  .2572E+00
  .2558E+00
  .2545E+00
  .2535E+00
  .2527E+00
  .2521E+00
  .2516E+00
PSI
KONTHS
.2776E+03
.2781E+03
.2786E+03
.2791E+03
.2795E+03
.2798E-»-03
.2802E+03
.2804E+03
.2807E+03
.2809E+03
.2810E+03
.2812E+03
.2813E+03
.2814E+03
.2815E-I-03
.2815E+03
.2816E+03
.2816E+03
.2816E+03
.2817E+03
.2817E+03
.2817E+03

     VLZZ
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
-.7009E+03
-.7025E+03
-.7171E+03
-.7330E+03
- . 7494E+03
-.7663E+03
-.7839E+03
-.8019E+03
-.8203E-f-03
-.8390E+03
-.8578E+03
-.8766E+03
-.8950E+03
-.9129E+03
-.9299E+03
-.9459E+03
-.9606E+03
-.9738E+03
-.9855E+03
-.9955E-K03
- . 1004E+04
-.1011E-K)4
- . 1017E+04
-.1022E+04
-.1025E-K)4
.1001E-01
.9930E-02
.9272E-02
.8608E-02
.7980E-02
.7381E-02
.6815E-02
.6282E-02
.5786E-02
.5325E-02
.4903E-02
.4519E-02
.4173E-02
.3866E-02
.3596E-02
.3362E-02
.3162E-02
.2992E-02
.2852E-02
.2736E-02
.2642E-02
.2567E-02
.2507E-02
.2461E-02
.2424E-02
.1743E+00
.1713E+00
.1505E+00
.1475E+00
.1411E+00
.1347E-t-00
.1280E+00
.1207E+00
.1131E+00
.1052E+00
.9693E-01
.8848E-01
.7992E-01
.7138E-01
.6299E-01
.5491E-01
.4728E-01
.4022E-01
.3383E-01
.2816E-01
.2324E-01
.1904E-01
.1554E-01
.1266E-01
.1032E-01
                              .6254E+04
                              .9466E+03
                              .1097E+03
                              .1001E+02
                              .7306E+00
                              .4309E-01
                              .2063E-02
                              .8033E-04
                              .2540E-05
                              .6503E-07
                              .1342E-08
                              .2218E-10
                              .2918E-12
                              .3034E-14
                              .2478E-16
                              .1579E-18
                              .7813E-21
                              .2990E-23
                              .8835E-26
                              .2015E-28
                              .3559E-31
                              .4890E-34
         lEVENT(l)- 0
               IEVENT(2)-
                                     135

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RELATIVE HUMIDITY OF THE OUTER BOUNDARY LAYER AIR- .1000E+01
RELATIVE HUMIDITY AT THE SOIL SURFACE- .9995E+00
SATURATED WATER VAPOR DENSITY IN AIR- .6016E-05
SATURATED WATER VAPOR DENSITY AT AIR-SOIL INTERFACE- .5983E-05
EVAPORATIVE(-) OR CONDENSIVE(+) FLUX- .3717E-04
AIR TEMP. AT OUTER BOUNDARY LAYER EDGE- .2764E+03
SURFACE INFILTRATION RATE(CM/HR)- .1000E-1-00
TEMP. RAIN WATER- .2770E+03
SOLAR RADIATION- .OOOOE+00
EVAPORATIVE(-) OR CONDENSIVE(+) HEAT TRANSFER- .2208E-01
SENSIBLE HEAT TRANSFER- .1920E-01
TRANSFER OF HEAT OF RAIN WATER INTO SOIL- .4000E+00
                                 136

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                     APPENDIX V: DISTRIBUTION OF SOFTWARE
The VIRTUS model software may be  obtained  from the Robert S. Kerr Environmental
Research Laboratory. Please  send a written  request with  either a 3.5 inch low
density (720 KB) or a 5.25 inch low density (360 KB)  diskette to the following
address. The diskette needs to be preformatted and MS-DOS compatible.


      VIRTUS Distribution
      Robert S. Kerr Environmental Research Laboratory
      U. S. Environmental Protection Agency
      P. 0. Box 1198
      Ada, OK 74821-1198
      USA
                                      137

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                                TECHNICAL REPORT DATA
                         (Please read Instructions on the reverse before completing]
. REPORT NO.
EPA/600/2-91/062
                  3. RECIPIENT'S ACCESSION NO.
                  PB92- 119 957
 TITLE AND SUBTITLE

 A MODEL OF VIRUS TRANSPORT IN UNSATURATED SOIL
                                                      5. REPORT DATE
                                                      6. PERFORMING ORGANIZATION CODE
 AUT.HORISI

  M.V. Yates,  S.R. Yates,  and XY.-Ouyang
                                                      I. PERFORMING ORGANIZATION REPORT NO.
. PERFORMING ORGANIZATION NAME AND ADDRESS
  Dept. of Soil  & Environmental Sciences, University of
  California,  Riverside,  CA   92501

 ^fSDA/ARS, U.S. Salinity Laboratory, Riverside, CA  92501
                  10. PROGRAM ELEMENT NO.

                   CBPC1A
                  11. CONTRACT/GRANT NO.

                   DW12933820
12. SPONSORING AGENCY NAME AND ADDRESS
 Robert S. Kerr Environmental Research Laboratory
 U.S.  Environmental Protection Agency
 P.O.  Box 1198, Ada, OK  74820
                  13. TYPE OF REPORT AND PERIOD COVERED
                   Research Report	
                  14. SPONSORING AGENCY CODE
                   EPA/600/15
15. SUPPLEMENTARY NOTES

 Project Officer:  David M. Walters
FTS:  743-2261
16. ABSTRACT

      As a  result of the  recently-proposed mandatory ground-water
 disinfection  requirements  to inactivate viruses  in potable water supplies;
 there has  been increasing  interest  in virus fate and transport in the
 subsurface.   Several models have been developed  to predict the fate of
 viruses in ground water, but few include transport in the unsaturated  zone,
 and all require a constant virus inactivation  rate.  These are serious
 limitations in the models,  as it has  been shown  that considerable virus
 removal occurs in the unsaturated  zone, and inactivation rate of viruses is
 dependent  on  environmental conditions.  The purpose of this  research was to
 develop a  predictive model of virus  fate and transport in unsaturated  soils
 that allows the virus inactivation  rate to vary  based on changes in soil
 temperature.   The model  was developed based on the law of mass conservation
 of  a contaminant in porous media and  couples the flow of water,  viruses,
 and heat through the soil.   Model predictions  were compared  to measured
 data of virus transport  in laboratory column studies,  and were within  the
 95% confidence limits of the measured concentrations.   Model simulations
 were performed to identify variables  that have a large effect on the
 results.
                             KEY WORDS AND DOCUMENT ANALYSIS
                DESCRIPTORS
                                          b.lDENTIFIERS/OPEN ENDED TERMS
                                 COSATi Field,Group
 Virus Transport
 Unsaturated Zone Modeling
 Pathogens
 Virus
 Heat Transport
18. DISTRIBUTION STATEMENT

  RELEASE TO PUBLIC
      19. SECURITY CLASS iTIm Report)
          UNCLASSIFIED
21. NO. OF PAGES
    148
                                          20. SECURITY CLASS fTliu page-

                                               UNCLASSIFIED
                                                                   22. PRICE
EPA Form 2220-1 (R«T. <-77)  PREVIOUS COITION n OBSOLETE
                   GOVERNMENT PJUNTlNGOmCE:! 9 92 Ji i a -003*0677

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