-------
U.S. EPA - CLP
10
HOLDING TIMES
Nama:
Coda:
C*«a Ho
Contrmct:
SAS No.:
SDG No.:
EPA
Saapla No.
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.
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'
POFM X - IN
7/87
B-118
-------
U.S. EPA - CLP
11
INSTRUMENT DETECTION LIMITS (QUARTERLY)
Name:
Coda:
Analy-f-tca.!
Case No.:
CP ID Number:
lam« AA ID Number:
urnace AA ID Number: P&3O3O
Contract:
SAS No.:
Data:
SDG No.
1
1
1
lAnalyte
1
| AluaimiM_
j Antimony_
1 Ars«nic
1 Barium
| B^rylliuji
|rii/tBlirH
| Calcium
| Chromium
1 Cobalt
1 Copper
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| Magn««ium
|Mangan*s«
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1 Nickel
(Potassium
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200
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25
100
5
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5000
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nfflents:
FORM XI - IN
B-119
7/87
-------
WDOE LABORATORY
ANALYSES
-------
GREGOIRE
Director
STATE OF WASHINGTON ^j
DEPARTMENT OF ECOLOGY ""V- •"--lG!l 10
Posf Office Box 3-46 • Manchester, Washington 98353-0346 • (206) 895-4740
October 13, 1988
TO: Dunne Kama,
Environmental Protection Agency
FROM: Bob Car re 11
SUBJECT: Ketchikan Pulp Company.
Hydrocarbon Identification
Nine samples were received at the Manchester Environmental Laboratory
for hydrocarbon identification. The method used is only for semi-
qualitative determinations of mixtures rather than individual
components.
Samples 350004, 350005, 350014, 390010, and 390103 all contained similar
late eluting compounds with boiling points generally higher than #2
diesel oil (i.e. 680°). Samples 350004 and 390010's components were
attenuated with respect to the others mentioned. In addition to the
afore mentioned components, samples 350D06, 350015 and 390106 also
contained #2 diesl oil and a series of early eluting compounds with
estimated boiling points from approximately 100°F to 350°F.
Sample 350013 contained only what appeared to be a lubricating oil.
MM/cm
B-120
-------
m PROGRAMS SECTION
EM PiSiQN 10
WASHINGTON STATE DEPARTMENT OF ECOLOGY
ENVIRONMENTAL INVESTIGATIONS AND LABORATORY SERVICES
MANCHESTER LABORATORY
TO: Duane Kama, EPA Region 10
FROM: Bob Carrell, Chemist
SUBJECT: Hydrocarbon Identifications on Ketchikan Pulp/Ward
Cove Sediment Samples
Twenty one samples, collected on August 22 and September 20 and 21,
1988, were received at the Manchester Environmental Laboratory on August
24 and September 22, 1988 for hydrocarbon identification. All of these
samples contained a cluster of high molecular weight hydrocarbons,
approximately C2Q-C23' wni-cn was not able to be identified. Samples
350303 and 350302 contained very little of these compounds while the
amount in the rest of the samples varied greatly.
In addition to these samples, 390121, 390120, 350308, 350307 and 350305
also contained detectable quantities of #2 diesel oil.
Lab Number
350301
350302
350303
350305
350307
350308
350309
350310
350311
350312
Ward Cove
Station I.D.
5-1
5-3
5-5
4-2
6-1
6-2
3-1
3-2
3-3
2-3
Lab Number
350313
350314
350315
350316
390117
390118
390119
390120
390121
390123
Ward Cove
Station I.D.
1-2
1-1
2-1
10-1
10-0
20-1
30-1
60-1
20-1
BC:mb
B-121
-------
APPENDIX C
ADEC FIELD NOTES AND RAW DATA
-------
STEVE COWPER, GOVERNOR
DEPT. OF ENVIRONMENTAL CONSERVATION
Dr. Harvey Van Veldhuizen, Project Manager
Jones and Stokes Associates, Inc.
1808 136th PLace, N.E.
Bellevue, Washington 98005
CERTIFIED MAIL
RETURN RECEIPT REQUESTED
Dear Dr. Van Veldhuizen:
November 2, 1988
re: EPA Contract NO. 68-02-4381
Ward Cove Long Term Data Summary
Enclosed is a brief description of our sampling project, field
observations, equipment information and an original and backup
floppy disk containing raw data. Also, please find maps depicting
observed drogue flow patterns and copies of the Ketchikan
International Airport Flight Service Station's daily weather
observations.
The raw data collected during the long term study is on the
enclosed floppy in an ASCII delimited file named wardcove.del.
Commas are used as the field delimiters. The record length is 310
entries long. The column definitions are included on a table
which also gives the data type and the length of the characters.
R-Base was used to create the data tables and to converted them
into ASCII delimited format. The data has been cross checked with
field notes to ensure accuracy. Field note narrations for all
sampling events are included on the floppy in WordPerfect,
version 4.2. The file name is fieldwrk. A brief introduction to
the data, methods used for its collection, calibration
techniques, and additional field observations are contained in a
WordPerfect file called WCLT.
You are welcome to phone us with questions on the data or its
interpretation.
Sincerely,
Amy Kruse,
Ecologist
Alex Viter-i,
Environmental Engineer
cc: Duane Kama, EPA/Region 10
Dan Bodien, EPA/Region 10
C-l
-------
Table: wc88
Read Password: No
Modify Password: No
Column definitions
# Name Type
1 samdate DATE
2 samtime TIME
3 transnum TEXT
4 sitenum TEXT
5 sitekey TEXT
6 botdepth REAL
No lock(s)
7 tide
8 temps
9 tempi
10 temp5
11 templO
TEXT
REAL
REAL
REAL
REAL
12 tempelse TEXT
13 sals REAL
14 sail REAL
15 sa!5 REAL
16 sallO REAL
17 salelse TEXT
18 dos REAL
19 dol REAL
20 do5 REAL
21 dolO REAL
22 doelse TEXT
23 notes NOTE
Length
Key Expression
2 characters
2 characters
10 characters yes
10 characters
4 characters
4 characters
4 characters
Current number of rows:
305
C-2
-------
WARD COVE 1988
LONG TERM WATER QUALITY
DATA SUMMARY
INTRODUCTION
The Department of Environmental Conservation, Southeast Regional
Office completed a long term water quality study of Ward Cove
during the summer of 1988. This study was conducted as part of
the joint EPA/State Ward Cove Water Quality Assessment Work
Assignment No. 68-02-4381. Contract negotiations, study design
review and field sampling were completed by Amy Kruse and Alex
Viteri. Water quality sampling occurred during neap tides in the
months of July, August, and September. The dates for each
sampling session were: July 23-27, August 5-10, August 18-24, and
September 12-18.
METHODS
The long term study followed the Quality Assurance/Quality
Control program contained in the work plan prepared by Jones and
Stokes Associates Inc., dated July 6, 1988. The QA/QC was
reviewed and approved by both DEC and EPA before field work
began. This study included measurements of dissolved oxygen,
temperature, and salinity at 16 study sites. Study sites on all
transacts are numbered from North to South, low to high. Samples
were taken at four depths, surface, 1, 5, and 10 meters. We
attempted to measure hydrogen sulfide and pH but were unable to
obtain reliable results due to interference with the sampling
technique from significant amounts of rain.
t
Dissolved oxygen and temperature measurements were taken with a
YSI model 57 dissolved oxygen meter. Salinity measurements were
taken with a YSI model 33 salinity meter. During the first two
weeks of sampling, salinity measurements were not taken at the 10
meter depth because the cable was not long enough. The salinity
value from the 5 meter depth was used again at the 10 meter
depth. Salinity measurements were taken at all depths during the
third and fourth weeks of sampling.
The salinity and dissolved oxygen meters were calibrated each
morning before sampling began. The dissolved oxygen meter was air
calibrated at ambient air temperature right before sampling
started. The DO meter was not adjusted for altitude because all
work was done at sea level. For the first two. weeks of sampling,
dissolved oxygen was measured in a glass of tap water that had
been overfilled for two minutes. A modified Winklers titration
using Hach kit OX-2P was completed on tap water also. The BOD
bottle was overfilled for two minutes and checked for excess
bubbles. The results of the Winklers Hach kit were consistently
2.0-2.5 mg/1 higher than the YSI meter readings. We consulted
with the Hach Company technical staff about this discrepancy-
They said that the PAO reagent was very sensitive to temperature
C-3
-------
changes and could have been harmed by temperature fluctuations in
transport. This would cause over titration of the endpoint. They
advised us to discontinue the measurements until new reagent
could be shipped. We took their advice. The salinity meter was
calibrated every day using a conductivity standard prepared by
the DEC laboratory of 0.7456 grams of KCL/1000 ml double
distilled water. The probe was submerged in the standard and the
measurement was recorded.
We calibrated the dissolved oxygen meter against a winkler
titration and another YSI dissolved oxygen meter model 54A on
August 3, 1988. The YSI model 57A used a hand calculated salinity
value. The model 57 has a salinity correction dial in the meter.
We used aged distilled water, stream water and seawater of 18 ppt
salinity for the calibrations. All winkler measurements were
performed on split samples. The results follow:
DO CALIBRATION
a. Distilled water
MODEL 57A
temperature 22 C
salinity 0
DO 8.6
b. stream water
temperature
salinity
DO
r
c. seawater
temperature
salinity
DO
MODEL 57A
16 C
0
9.8
MODEL 57A
16.5 C
18 ppt
7.5
MODEL 57
22 C
0
8.7
MODEL 57
16 C
0
9.8
MODEL 57
16.5 C
17ppt
7.7
WINKLERS TITRATION
N/A
0
9.2, 9.2
WINKLERS TITRATION
N/A
0
10.55, 10.58
WINKLERS TITRATION
N/A
N/A
8.2, 8.2
The sampling procedure for all water quality measurements were as
follows; lower probe to sampling depth, measure water
temperature with DO meter, adjust temperature dial on salinity
meter, read salinity, adjust salinity on DO meter and read DO.
The DO probe is slowly moved up and down while measurements are
taken to assure that there is a mixing of the, water in contact
with the probe membrane. All measurements were recorded when the
meter needles stopped moving.
KPC assigned a field crew for the entire study to copy every one
of our water measurements. They duplicated our transacts and
depths except for the 10 meter readings. Their gear was not long
enough for that depth. They grabbed water samples at depth with
sample bottles and brought them to the surface to do the
C-4
-------
readings. They stated that they normally did not work at as fast
a pace as we were able to go, but tried to keep up with us. We
noted that there was agitation of the water samples while they
were measuring for salinity and oxygen values.
drogue study:
Drogues were constructed from 8 inch white styrofoam cylinders
purchased at a near by hardware store. The cylinders were cut
into eight inch wide by 2 inch deep rings. Two rings were tied
together with fish line so that they had about one foot
separation. Bright yellow field flagging tape was wrapped around
each ring to increase visibility. A 16 inch diameter toy punch
balloon was filled with fresh water and tied onto one of the
rings with sufficient line to allow two feet separation. Please
see attached photo. The drogues appeared to follow surface
currents with minimal wind interference. Maps depicting drop off
and pick up points for the drogues are attached.
weather:
Through out the majority of the study the weather was
predominately cold, with heavy rain fall, dense overcast and
changing high winds. Attached are copies of Ketchikan
International Airport FAA Flight Service Station's daily weather
observations for the months of June, July, August, and
September. Incorporation of the weather information is mandatory
for proper water guality assessment of Ward Cove. Over the first
sampling week, heavy rainfall created a cove freshet. We believe
that water quality data collected during the freshet can be used
as base line of the cove's surface water quality.
stream flow:
Lack of proper field instrumentation made it impossible for us to
measure Ward Creek stream flows. However, visual observations
were made as time and weather allowed. Please see field notes.
transect and study sites:
A map of transect and study sites used during the study is
attached. A video tape of transect end points is also available.
Sample sites used by KPC for their discharge monitoring reports
do not directly correspond to the study sites used in the long
term study. However, many of the sites are the same. See
attached transect/study site map.
log rafts:
The number and size of log rafts stored in Ward Cove changed
significantly during the course of the study^ Through out the
first week of the study, the number and size of rafts in the cove
appeared normal. Starting the third week of the long term study,
which was coincident with EPA's intensive study, the number and
size of rafts in the cove decreased significantly. A video tape
of log rafts contained in the cove during the intensive study is
available. The number or size of log rafts in Ward Cove at during
the last two weeks of our study were less than anytime in the
last eight years (per. comm. Alex Viteri).
C-5
-------
-------
WARD COVE
\
- Drogue Sites (Paired)
- Additional Hydrographic
Stations
- Existing Hydrographic
Stations
Figure 1. Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
C-7
-------
Vh
rti
WARD COVE
3-7,
Q /j .-
X
*
^
•^
o
^
Vlv
K
•
SI
I
- Drogue Sites (Paired)
- Additional Hydrographic
Stations
- Existing Hydrographic
Stations
•SOS
Figure 1, Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
C-8
-------
WARD COVE
- Drogue Sites (Paired)
- Existing Hydrographic
Stations
- Additional Hydrographic
Stations
Figure 1. Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
C-9
-------
•R*
•^
O
W
1
•
SI
/
- Drogue Sites (Paired)
- Additional Hydrographic
Stations
- Existing Hydrographic
Stations
%.
Viv
Figure 1, Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
c-io
-------
WARD COVE
- Drogue Sites (Paired)
- Additional Hydrographic
Stations
- Existing Hydrographic
Stations
Figure 1, Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
c-n
-------
WARD COVE
Q/i'.2£>f*i
- Drogue Sites (Paired)
- Additional Hydrographic
Stations
- Existing Hydrographic
Stations
Figure 1, Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
C-12
-------
- Drogue Sites (Paired)
- Additional Hydrographic
Stations
- Existing Hydrographic
Stations
•sea
Figure 1, Locations of drogue placement and hydrographic stations
in Ward Cove and Tongass Narrows,
C-13
-------
0
I
GENERAL DESCRIPTION
The YSI Model 57 Dissolved Oxygen Meter is intended for dissolved oxygen
and temperature measurement in water and wastewater applications, but is also
suitable for use in certain other liquids. Dissolved Oxygen is indicated in mg/l
(milligrams per liter) on 0-5. 0-10 and 0-20 mg/l scales. Temperature is indicated
in °C on a -5° to +45°C scale. The dissolved oxygen ranges are automatically
temperature compensated for solubility of oxygen in water and permeability of
the probe membrane, and manually salinity compensated.
The probes use Clark-type membrane covered polarographic sensors with
built in thermistors for temperature measurement and compensation. A thin.
permeable membrane stretched over the sensor isolates the sensor elements
from the environment, but allows oxygen and certain other gases to enter. When
a polarizing voltage is applied across the sensor, oxygen that has passed through
the membrane reacts at the cathode, causing a current to flow.
The membrane passes oxygen at a rate proportional to the pressure difference
across it. Since oxygen is rapidly consumed at the cathode, it can be assumed
that the oxygen pressure inside the membrane is zero. Hence, the force causing
the oxygen to diffuse through the membrane is proportional to the absolute
pressure of oxygen outside the membrane. If the oxygen pressure increases,
more oxygen diffuses through the membrane and more current flows through the
sensor. A lower pressure results in less current.
SPECIFICATIONS
I. Instrument
Oxygen Measurement
Ranges: 0-5, 0-10 and 0-20 mg/l (0-2.5, 0-5 and 0-10 mg/l with YSI
5776 High Sensitivity Membrane)
Accuracy: ±1% of full scale at calibration temperature (±0.1 mg/l on 0-10
scale), or 0.1 mg/l (whichever is larger).
Readability: .025 mg/l on 0.5 scale: .05 mg/l on 0-10 scale; 0.1 mg/l
on 0-20 scale.
Temperature Measurement
Range: -5° to f45°C
Accuracy: ±0.5°C plus probe which is ±0.1 °C
Readability: 0.25°C
Temperature Compensation
±1% of D.O. reading for measurements made within ±5°C of calibration
temperature.
±3% of D.O. reading over entire range of -5 to +45°C probe temperature.
System Response Time
Typical response for temperature and D.O. readings is 90% in 10 seconds
at a constant temperature of 30°C with YSI 5775 Membranes. D.O.
response at low temperature and low D.O. is typically 90% in 30 seconds.
YSI 5776 High Sensitivity Membranes can be used to improve response at
low temperature and low D.O. concentrations. If response time under any
operating conditions exceeds two minutes, probe service is indicated.
Operating Temperature Range
Instrument and probe operating range is -5° to +45°C. Large ambient
temperature changes will result in 2% loss of accuracy unless Red Line and
Zero are reset
Recorder Output
0 to 114-136 mV. Recorder should have 50.000 ohms minimum input im-
pedance.
Power Supply
The YSI Model 57 is powered by two disposable "C" size carbon zinc
batteries (Eveready 935C or equal) providing approximately 1000 hour
operation.
II. Probe
Cathode: Gold
Anode: Silver
Membrane: .001" FEP Teflon
( 0005" FEP Teflon available)
Electrolyte Half saturated KCI
Temperas Compensation: (See SPECIF.CATIONS, I. Instrument)
Pressure Compensation: Effective 1/2% of reading w.th pressures to 100
psi (230 ft. sea water)
Polarizing Voltage: 0.8 volts nominal
Probe Current: Air at 30°C = 19 microamps nominal
Nitrogen at 30°C = .15 microamps or less
III. Accessories and Replacement Parts
YSI 5720A — Self Stirring BOD Bottle Probe
YSI 5750— Non Stirring BOD Bottle Probe
YSI 5739 — Oxygen Temperature Probe for field use. Combine with one of
the following 4 cables for desired lead length:
Detachable leads for use with YSI 5739:
YSI 5740- 10 10' Cable
YSI 5740- 25 25' Cable
YSI 5740- 50 50' Cable
YSI 5740-100 100' Cable
YSI 5740-150 150' Cable
YSI 5740-200 200' Cable
YSI 5721 —Battery and charger pack operates YSI
Submersible Stirrers.
YSI 5791A — Submersible "Stirrer for field use.
YSI 5795A — Submersible Stirrer.
YSI 5075A — Calibration Chamber for use with field probe,
YSI 5890 — Carrying Case.
YSI 5775 " Membrane and KCI Kit, Standard — includes 2 each 15-
membrane packets (.001" thick standard membranes) and a
30 ml bottle KCI with Kodak photo flo.
YSI 5776 — Membrane and KCI Kit. High Sensitivity — includes 2 each
1 5-membrane packets (.0005" thick membranes) and a 30 ml
bottle KCI with Kodak photo flo.
YSI 5680 — Probe Reconditioning Kit
5791A and 5795A
-------
FIELD NOTES FOR THE LONG TERM STUDY
REVIEW OF NOTES IS IMPORTANT FOR PROPER DATA INTERPRETATION
NOTES FOR 7/23/88:
10:00 am The weather is cloudy, steady rain with approximately
20 mph winds from several directions at once. Wind gusts to 35-40
mph. Yesterday was partly cloudy with only minor showers. High
tides for 7/23/88 are 9.6 at 8:24 am, 13.2 at 8:11 pm. Low tides
are 3.1 at 2:04 am and 5.4 at 1:45 pm.
NOTES FOR 7/25/88;
12:00 pm. new batteries for both DO meter and salinity meter.
Calibration of the salinity meter; temperature of water is 18 C,
salinity is 1 pp thousand, conductivity read on the X 10 scale.
Meter reading is 130 x 10. Calibration of DO meter; new membrane,
air calibrated meter, measured DO in tap water with the YSI meter
and a Hach dissolved oxygen modified field kit. Tap water
temperature is 21.5. YSI reading on 0-10 scale - 7.8. Air
temperature is 20 C. Hach kit reading on tap water was 11.0.
2:15 pm Set the drogues out by the effluent outfall boom
approximately 30 feet from the boom. Drogue A is even with the
secondary treatment facility, drogue B is across from the
effluent pipe. Kurt Mueller and Steve Eilertson are sampling for
KPC again.
High tide is at 11:06 am low tide is at 4:14 pm.
(Rain fall for this period is very heavy and long in duration.
Please reference FAA's daily weather charts.)
NOTES FOR 7/27/88;
t
We are using the District office's salinity meter. Calibration of
salinity meter with standard; standard temperature 18 C, salinity
of standard 0.5, conductivity on X 10 sale is 130. Calibration of
DO meter; air temperature 21.5, meter reading is 9.3, calibrate
to 8.8. Compare DO readings of tap water with YSI meter and Hach
modified winklers test kit. Tap water temperature 17 C. YSI
reading is 10.1. Hach kit reading is 12.
Set drogues out at 10:45 am. Used same locations for A and B as
7/25 except they were approximately 40' out from effluent log
boom because a barge was being loaded. Its engines were running
and effecting the surface currents. Weather is dry, some sunshine
this a.m., about two hours. Now its overcast. Rain decreased in
last 24 hours. Wind is very gentle. Air temperature is 15 C.
Drogue C is dropped by mouth of creek in line with telephone pole
on transect one and the log boom by mill. Drogue B is at transect
three station one at 1:25. Drogue A probably went into log boom
area by north side of cove at effluent outfall. Took photos of
stream at 2:30 pm. No fish or fishermen observed. No rain this
pm. High tide at 12:47 pm 13.1', low tide at 6:10 pm 3.7'.
C-15
-------
NOTES FOR 8/5/88;
Air calibration of the DO meter to 22.5 C, meter reading is 8.2,
calibrated to TABLE I value of 8.6. Thermometer reading for room
temperature is 23 C. Calibration of salinity meter using
conductivity standard made up by Lab. Salinity of standard is 1.5
pp thousand, conductivity on the X 10 scale is 125 micro mhos.
Recalibrated the DO meter to the outside air temperature of 19 C.
Calibrated to 9.26 from TABLE I.
4:00 pm drogue A is released by effluent outfall at site 43.
Drogue B is released in line with the secondary treatment
facility. Gas bubbles are rising in this area a lot. Weather is
high overcast without rain about 60-65 F. Wind is blowing WNW.
4:30 pm drogue A about 70' off of the log boom by effluent
outfall. Drogue B is in middle of log rafts with the diesel tank
on it.
5:00 pm released drogue C at stream mouth. Wind blowing east.
6:00 pm no wind, water is smooth as glass. Drogue C is even with
the stairs going down to the boom shack, in the middle of the
cove.
8:20 pm Drogue A in center of cove midway between transect 2 and
3 and across from the diesel tank. Drogue C is caught on the
upstream side of a log boom across from the log bundle lift
approximately 30' inside the end of the boom. Can't find B.
Air calibrated the DO meter at end of sampling, 8:30 pm. Air
temperature is 13.5 C, meter reading 7.1, calibrated to 10.4 from
TABLE I.
7:30 pm talked to Kurt Muller and Steve Eilertson. They were also
getting low DO values on bottom by stream mouth (3.3 ppm) and the
surface by the effluent outfall and boom shack. They also skipped
some sites in order to keep up with us. The water from our boat
wake is really foaming and the foam is persistent for a long
time. Kurt said that he didn't have his map with him so his
sampling locations were different from the established ones.
Low tide is 12:55 pm at 4.3', high tide is 7:32 pm at 14.3'.
NOTES FOR 8/6/88
11:40 am Kurt Muller and Suong Yu(?) are sampling for KPC. Suong
has never sampled before. High overcast with very vague shadows.
About 65 F, very calm. Smoke from mill stacks is going straight
up. Air calibration of DO meter; temperature^of air is 20 C, DO
reading on 0-10 scale is 12.0. Let machine sit for 20 minutes to
equilibrate. Calibrate to 9.07 from TABLE I. New membrane for DO
meter.
Released drogue A at outfall at 12:05 pm. Only have two drogues
today. Gas bubbles are still coming up by outfall. Seagulls and
eagles feeding on fish on surface. Drogue C released across from
bulkhead in line with stream mouth at 12:15. Drogue C was found
C-16
-------
almost against the shore at bulkhead at 12:40. Drogue A is no
where to be found at 1:15.
3:30 Drogue float spotted hanging from dead tree in the boomed
off area of the effluent discharge area. They were hanging about
3' above the water at low tide. Don't know which drouge they are-
can 't get to the site. 4:30 drogue C found aground near bulkhead
towards the road.
Surveyed Ward Creek just above where the stream enters the tide
pool area. The stream bottom substrate was predominately bedrock
with cobble, rubble and boulders on top. The average depth was 3-
4" with some 6-8" areas. The small falls area has a drop of 10-
12". Three widths were taken in a 200' span moving upstream from
the small falls area. The widths were 65', 83' and 96'. The
stream temperature was 16 C. Three DO reading were taken across
the stream, they are 8.7,8.8 and 8.8. The stream flow has changed
dramatically since the last field trip in July. In July, after
heavy rains for several days straight, the small falls into the
tide pools had a drop of 2.5', and the stream was running bank
full. The July stream survey was done at low tide. At that time
stream flow was so fast that had a person fallen into the water
they could not have recovered in time to get to shore.
NOTES FOR 8/8/88
2:00 Air calibration of the DO meter; 15.5 C air temperature,
meter read 9.8, corrected to 9.9 mg/1 from TABLE I. New membrane
for the probe. Calibration for the salinity meter; meter reading
22 C, salinity of standard is 1.4 pp thousand, conductivity
reading is 145 micro mhos.
Weather on Sunday was very windy with lots of rain. Weather on
Monday is very similar, perhaps not as much wind and rain as
Sunday. 3:00 pm rain stopped. Wind is WSW at about 10 mph. Still
low overcast clouds.
Drogue A is released by effluent outfall 3:00 pm. Drogue B is
released by secondary treatment facility at 3:05 pm. Started
sampling at (KPC's) reference stations (50 and 51 outside Ward.
Cove) so that we could be by the stream mouth at low tide.
3:30 pm waves coming into Ward
Cove from Tongass Narrows 8-12" high. Dropped drogue C off at
stream mouth at 4:25 pm. Drogue A is along shore by pilings at
C-17
-------
stream mouth at 4:30 pm. Drogue B is along effluent boom heading
toward anchored barges at 4:30 pm. Drogue C is in raid-cove by
diesel tank across from grey warehouse at 5:00 pm. Drogue C was
found at the diesel shack in middle of cove. Drogue B is next to
effluent boom close to barges. Drogue A was caught under rocks by
dock next to outfall, it lost one of its floats.
NOTES FOR 8/9/88;
Weather is dryer - not much rain, moderately low hanging clouds.
Wind from SSW at 15-18 mph. Occasional (higher) gusts. Light
rain. While outside Ward Cove, at KPC's sample site 51, near
float plane ramp, currents were pushing boat out while wind was
heading in. New batteries for both salinity and DO meter, new
membrane for DO meter. Air calibrate DO meter; 16.5 C, meter
reads 11.2, calibrate to 9.75 mg/1 from Table I.
2:15 pm Drogue C is released by effluent outfall. 2:45 pm drogue
C is picked up close to dock by effluent outfall. It was about to
go under the dock face. 2:45 released drogue on line with
transect 3 perpendicular to study site two on transect two.
Drogue A from Saturday is found between the two barges along the
NW shore. Drogue A from last trip was found wrapped around a
cable inside of the effluent foam boom. 6:00 pm drogue C is
located at effluent outfall, it made a circle in last 1.5 hours.
Gas bubbles coming up by effluent outfall again, they seem more
prevalent at low tide.
Tides today; low 5:46 pm at 4.7 feet, high 12:20 pm at 12.2 feet.
NOTES FOR 8/10/88:
2/25 pm Weather is partly cloudy approximately 65 (degrees F). No
rain last night. Wind SW at 3-5 mph. Lots of brown color in the
water, even at the reference points. Our boat wake creates lots
of foam and is very persistent. Seems to be worse than yesterday.
New membrane for DO meter. Air calibrated the DO meter.
2:25 pm released drogue A at a point on transect 3, perpendicular
to transect 2, study site 2. 2:40 pm released drogue C at
transect 1 study site 1. Drogue C was found at 5:10 pm up on a
rock behind pilings at creek mouth by sawmill floating dock.
Drogue A was found at 5:15 pm about 200 feet north of first site
on transect 2 in between two barges. It moved counter clockwise
inside log booms and along side the big dock.
I think the DO values have dropped during the 4 hour sampling
period today. May be due to the sun's warmth?
Tides today; low at 6:28 pm, high at 1:01 pm 13.0 feet.
C-18
-------
NOTES FOR 8/20/88: JOINT LONG TERM & INTENSIVE STUDIES UNDERWAY;
11:30 am Weather is high clouds, very scattered showers, wind is
from NE. Water is flat calm. Deploying the productivity buoys to
measure primary productivity and the mini-ranger floats. We
sampled KPC reference sites (No. 50 and 51 outside Ward Cove),
then transect 4, transect 1, transect 2, transect 3. Sampling was
conducted in this order because of boat and barge activity in the
area. The KPC crew sampled transect 3 right after transect 4,
around 2:00-2:30 pm. KPC crew is taking samples off of the stern
of their big boat, the Tongacell. The samples may be effected by
the boat propellers- increase in DO?
At the fifth site on transect 3 we noticed that the particles on
the very surface were moving towards the creek while those
approximately five inches down were moving out of the cove. The
salinity on the top one inch was 15 ppt, and five inches down was
20.5 ppt.
Air calibration of the DO meter on 0-20 scale. Meter reading was
11.0 mg/1 at 15 C. Calibrated meter to 10.07 mg/1 from Table I.
We started sampling at the reference sites and moved in toward
the stream. Tides today; low at 11:50 am 5.0 feet, high at 6:14
pm 13.2 feet.
NOTES FOR 8/21/88:
Weather is high overcast and wind has picked up on outside
reference points. Started sampling at the reference stations at
11:15 am from the Curlew with the Kinnetics crew taking identical
samples with their CTD at the same time. The KPC Tongacell is
also running their samples along with ours. We (Amy Kruse and
Alex Viteri) started sampling from the 10 meter deep study site
to let the surface waters equilibrate as long as possible after
boat disturbance. We are sampling from the starboard bow of the
Curlew. (Alex Viteri is locating the Curlew over established
study sites)
DO meter calibration at 16 C, meter reading 9.2 calibrate to 9.85
mg/1 from Table I. We completed sampling KPC reference sites
(No.50 and 51 outside Ward Cove), transect 6, transect 4 and site
one on transect three. The DO meter is giving very low readings.
We had a lot of hydrogen sulfide gas bubbles at the effluent
outfall station, site one on transect 4. We changed the membrane
and re-calibrated the DO meter at 2:40 pm. at_ 15.5 C. The meter
was reading 7.7, calibrated to 9.9 mg/1. We re-sampled all study
sites again. We will not use the data from the first sampling
period.
Tides today; low 12:56 pm at 6.0 feet, high 7:22 pm at 12.9 feet.
C-19
-------
NOTES FOR 8/22/88;
Weather was high clouds with some blue patches. We (Amy Kruse and
Alex Viteri onboard the AFF&G skiff) started sampling at the
creek with the Tongacell right beside us. Beth Power from EVS
consultants is aboard the Tongacell to observe their procedures.
Alex and I had a meeting with several KPC people; Hagen, Higgins,
-Steve Eilertson, Soung Yu and Beth Powers. They asked about what
the intensive study was doing. We explained it. We talked further
about what our results had been so far for water quality. We
explained that we had some consistent depression of DO at the
surface and that there were locations by the stream that were
very depleted of oxygen. Hagen asked the KPC field crew if their
results were similar. Hagen asked to
meet with Kinnetics and Larry Larsen to discuss our studies. We
said we would pass the request along. Beth asked to be allowed to
come aboard the Curlew to assess the gear and techniques for
sampling. She was allowed to come aboard and help sample for
awhile.
We re-air calibrated the DO meter after the first transect
because we were worried about interference from hydrogen sulfate
and its effects on the membrane at the site by the stream with
the really low DO values. The DO meter was perfectly calibrated
with the Table I values. Our readings on the surface were very
depressed, mostly 5-6 mg/1. About 60 % of our DO values were
below 6 mg/1, violations of the Water Quality Standards.
Tides today; low 2:32 pm 6.3 feet, high 9:31 pm 9.7 feet.
NOTES FOR 8/23/88:
Easterly winds, clear skies today for most of the day. Air
calibrated the DO meter at 19.5 C, meter reading 8.7 mg/1,
calibrated to Table I value of 9.17 mg/1.
Tides today; low 3:58 pm 5.7 feet, high 10:48 am 10.9 feet.
NOTES FOR 8/24/88:
Weather is dry and partly cloudy- We did not sample all of the
study sites today because we had an early morning meeting with
KPC, Kinnetics and Larry Larsen, and we had to catch an early
afternoon plane.
Air calibration of the DO meter at 17 C, meter reading 9.0 mg/1
calibrated to 9.65 mg/1 from Table I.
Tides today; low 5:03 pm 4.3, high 11:41 am 12.4.
C-20
-------
NOTES FOR 9/13/88:
Weather is low, heavy overcast skies. Winds shifting from N to SE
and increasing from 15 to 20 mph. Heavy drizzle.
Air calibrated the DO meter at 13 C. Meter reading 15.2 mg/1,
calibrate to 10.52 mg/1 from Table I.
Tides today; low 8:28 am 0.6 feet, high 2:39 pm 15.6 feet.
NOTES FOR 9/14/88;
Weather was sunny in the morning, started to cloud up about noon.
Water is calm. We (Amy Kruse and Alex Viteri) sampled some study
sites out of order because there was a lot of log raft movement
today and they were in the way. KPC crew did not sample today
because they could not use the Tongacell and did not want to use
the generator in the rain.
Air calibrated the DO meter at 15.5 C. Meter is reading 9.1 mg/1,
calibrate to 9.9 mg/1 from Table I.
NOTES FOR 9/16/88;
Weather is clear with some high clouds. Wind blowing hard 20 mph
frpm the Northwest. Scot Menzies is running the boat today for
our crew, Alex Viteri is operating the instruments.
Air calibrated the DO meter at 15 C. Meter reading 9.9 mg.l re-
calibrated to 10.07 mg/1 from Table I.
FIELD NOTES FOR 9/17/88;
Weather is partly sunny today. Yesterday was sunny with strong
Northern winds. The inner cove is calm, the outer cove has wind
driven waves. We are sampling from the Tongacell today because
our salinity meter is broken and we are using their's. It is the
same model, a YSI 33. (Alex Viteri is guiding the Tongacell
skipper to our sample sites and Amy Kruse is operating the
instruments.)
Air calibration of the DO meter at 15.5 C. Meter reading 12.4
mg/1, re-calibrated to 9.95 mg/1 from Table I.
We did not sample the third site on transect one because the
Tongacell can't get in there.
C-21
-------
12:10 pm We are noticing a lot of grey pulp in the water
throughout the cove. We suspect the pulp mill emptied their high
density tanks into the cove. Also, there are a lot of gas bubbles
coming up by study site one on transect two.
12:50 pm Study site two, transect three. We re-air calibrated the
DO probe to see if the high concentration of pulp fibers in the
water has effected the probe. Air temperature is 16.8 C, meter
reading 9.8 mg/1, Table I value is 9.7 mg/1.
Tide today; low 10:29 am at 4.6 feet, high 4:41 pm at 14.0 feet.
C-22
-------
TEMPERATURE SALINITY DISSOLVED OXYGEN
MONTH
7
7
7
7
7
7
7
7
7
7
7
7
7
7
0 7
1 7
M 7
OJ 7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
DAY YEAR HOUR MINUTE STATION DEPTH
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
25
25
25
25
27
27
27
27
27
27
27
27
27
27
27
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
11
11
12
12
12
12
12
12
12
1
1
1
1
1
11
11
10
10
2
2
2
2
10
11
11
12
12
12
11
11
11
11
12
30
45
0
10
20
30
40
45
50
0
10
15
30
40
0
15
25
45
40
40
45
55
55
5
50
0
po
25
10
30
40
45
50
21
23
24
25
31
32
33
34
35
43
42
41
55
51
13
22
11
12
11
12
13
21
11
12
24
25
31
32
13
21
22
23
42
17
48
51
90
25
90
90
0
90
0
75
0
0
0
9
35
30
27
29
0
10
31
30
0
0
38
999
999
0
39
40
999
999
TIDE (surf.)
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
outgoing
14
14. 5
15
14.5
15
14 .5
14
14
14.5
14.5
14.5
14.5
14
14
15
14
15
15
13
13
13
12.5
13.5
13.5
13.5
14
14
14.5
13.5
13.5
13
13
14
(1 m)
14
14
14.5
14
14. 5
14 .5
14
14
14
14.5
14.5
14.5
14
14
14.5
14
15
14.5
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13.5
(5 m)
13
13
14
13.5
13
14
13.5
13.5
13.5
13
13.5
13.5
13.5
13.5
14
13
13
12.5
13
13
13
13
13
13
13
12
12.5
13
12.5
12.5
12.5
12
(10 m)
12
12
12.5
13
14
13
12
12
12
12
12
13.5
12
12
12.5
12
12
12
11.5
11.5
11.5
11.5
11.5
11.5
11.5
11
11.5
11.5
(surf.)
22.5
21
22.5
23
20.5
22
23
24
24
23
23
23
22
22
22
22.5
17
22
5
8
3
9
7
1
8
16
9
20
2
19
21
5
21
(1 m)
22
23
23
23.5
23
24
23.5
24.5
24
23.5
23.5
23
23
23
23
23
23
23
9
13
8
17
23
22
23
.23.5
23
22.5
22
22.5
23
22
23
(5 m)
24
24
23
24.5
25
25
24
24.5
24
25
24
24.5
23
25.5
23
23
24
25
23
23
21
23
23
23.5
24
23
23
23
23
24
24
23.5
(surf.)
6
6.1
6
6.6
6
6
5.8
5.9
6.2
6.3
6
6.5
6.4
7.4
5.7
6
7
5.7
9.2
8.9
9.1
7.9
8.8
10.1
7.3
7.5
7.7
7
10.2
8.3
7
8.8
6.7
(1 m)
6.7
6.5
6
7.1
6
5.8
6.3
6
6.7
6.5
5.8
6.5
6.5
7.4
6.4
6.4
5.7
6.2
9
8.2
3.5
7.5
8.1
8.3
8
8
7.1
7.4
8.3
7.5
7.4
7.9
6.8
(5 m) (10 m)
4.9
6.4
7.1
7.6
6.5
6.8
7.4
7.4
7.7
7.5
7.4
7.1
7.7
8
6.2
7.8
7.2
7.7
7.6
6.7
7.6
7.5
7.9
7.8
7.5
7.2
7.3
7.5
7.1
7.2
7.9
7.5
7.8
6.4
8.1
6.8
7.7
7.6
8
8.2
8.1
7.8
7.7
8.2
7.6
1.5
3.7
4
3.5
6.7
5.7
6.4
6.7
7.2
7.6
7.5
6.2
6.5
6.9
7.8
-------
TEMPERATURE SALINITY DISSOLVED OXYGEN
MONTH
7
7
7
7
7
7
7
8
8
8
8
8
8
8
0 8
1 8
M s
Jx^
^ 8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
DAY YEAR HOUR MINUTE STATION DEPTH
27
27
27
27
27
27
27
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
12
1
1
12
12
12
12
5
5
5
5
4
4
4
4
6
6
6
7
7
7
7
7
5
6
6
6
12
12
12
1
2
1
55
5
10
20
15
10
45
25
30
35
45
25
15
45
50
25
15
10
5
15
30
40
50
50
0
45
35
25
35
50
5
0
50
43
55
51
33
34
35
41
22
21
23
24
11
36
12
13
33
34
35
41
42
43
51
50
25
26
31
32
11
13
21
22
32
33
999
999
999
999
999
38
75
41
999
89
999
21
100
999
12
999
999
29
100
999
999
999
999
999
999
33
999
27
7
999
999
999
999
TIDE (surf.)
outgoing
outgoing
outgoing
incoming
incoming
incoming
slack
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
slack
slack
outgoing
incoming
incoming
incoming
incoming
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
13.5
14
14
14
14
14
14
15
15
15
15
15.5
16
15
15.5
15
16
16
15.5
16
15.5
15
15
15
15.5
15.5
15.5
15
15
15
15
15
15
(1 m)
13
13.5
14
13
13
13.5
13
15
15
15
15
15.5
15.5
15.5
15.5
15
15
15
15.5
15.5
16
15
15
15
15
15
15.5
14.5
14
14
13.5
14
14
(5 m)
12
13
12.5
12.5
12.5
12
12
14.5
15
14
15
14
14.5
14.5
14
14.5
14
14
14.5
14
13
14
14
14.5
14
14
13
13
12.5
13
12.5
(10 m)
11.5
12
11.5
11.5
11.5
11.5
11.5
12
12.5
12
12.5
12
12
12
13.5
12
12
12
12
12
12.5
12
12
12
12
12
12
12
11.5
12
(surf.)
20
22.5
21
22
17
17
14
12.5
20
19
19.5
9
19
19
10
20
20
17.5
20
19
20
20
21
20
20
20
20
20
19
19
19
20.5
20.5
(1 m)
23
23
23
22.5
24
23.5
23
20
20
20
20
20
19
20
20
20
20
20
20
21
20
21.5
21
20
20
20
20
21
21.5
22
22
21.5
21.5
(5 m) (surf.)
24
24
24
23.5
25
24
24
21.5
20
20
20
20
21.5
20
21
21
21
21
21
21
21.5
21.5
21
20
21
21
23.5
24.5
24
25
25
7.3
7.6
7.4
7.5
7
7
7.7
5.4
5.4
5.3
5.3
6.8
6.4
5.6
6
5.2
5.3
5.2
5.5
5.2
5.6
6
6
5.6
5
5.1
5.4
5.1
5.4
5.1
5.6
5.7
6
(1 m)
7.7
7.4
7.8
7.9
7.7
7.6
7.6
5.9
5.8
5.5
5.6
6.6
6.5
6
5.5
5.4
6.3
6.3
5.7
5.7
5
7
6.3
6.8
6.5
5.1
5.9
6
5.4
6
6.5
5.4
6.4
(5 m) (10 m)
7.4
7.8
8
7.9
7.8
8
7.4
6.6
6.2
6.7
8.1
7.2
7.2
7.4
7.7
7.6
7.5
6.1
8.3
6.8
7.5
7
7.8
7
5.8
6.3
6.8
7.1
8.3
7.7
8.2
7.7
8.2
8.2
6.6
8.2
7.5
7.9
7.3
6.4
6.6
6.9
7.4
3.8
7.1
7.1
8.5
6.1
9.1
8
8.4
8
7.7
6.7
5.1
7.4
2.7
4.5
4.4
7.5
6.3
-------
TEMPERATURE SALINITY DISSOLVED OXYGEN
MONTH
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
0 8
tli 8
S 8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
DAY YEAR HOUR MINUTE STATION DEPTH
6
6
6
6
6
6
6
6
6
6
6
6
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
1
1
2
2
3
3
12
2
1
2
1
1
4
4
5
5
5
5
5
5
3
3
4
4
6
4
4
5
5
5
3
3
3
45
40
50
55
10
20
30
55
30
5
15
20
15
45
15
20
30
40
45
50
50
55
5
10
0
30
,55
0
5
10
20
30
40
34
35
41
43
50
51
12
42
25
31
23
24
34
22
11
12
13
14
15
16
41
31
32
33
17
35
21
23
24
25
50
51
43
999
27
999
999
999
999
9
0
40
24
999
999
100
32
28
27
6
20
17
17
999
25
999
999
8
28
32
999
53
40
999
999
999
TIDE (surf.)
outgoing
outgoing
outgoing
incoming
incoming
incoming
outgoing
incoming
outgoing
outgoing
outgoing
outgoing
outgoing
slack
incoming
incoming
incoming
incoming
incoming
incoming
outgoing
outgoing
outgoing
outgoing
incoming
outgoing
slack
incoming
incoming
incoming
outgoing
outgoing
outgoing
15.5
15.5
15.5
15
14
15
15
15
15
15.5
13 .5
15
13.5
14
13.5
13
13.5
13.5
13.5
13
14.5
14
13.5
13.5
13.5
14
13. 5
13.5
13
13.5
14
13.5
14
(1 m)
14
13
15
14.5
14
14.5
14.5
14
14
14.5
13
14
13.5
13
13
13.5
13.5
13
13
13
14
13.5
13.5
13
13
13.5
13
13
13
13.5
13
13.5
14
(5 m)
13
12.5
13
13
12
13
12
12
13
12
12.5
13
13
13
13
13
13
13
13
12.5
13
13
13
13
13
13
13
13
13
13
(10 m)
11.5
12
11.5
11.5
12
12
12
11.5
12
11.5
11.5
13
13
13
13
12.5
13
13
13
13
13
13
(surf.)
20
20.5
21
21
22.5
21.5
17.5
21
19
20
20.5
19
11
14
10
7
8
7
7
7
21
12
16
15
10
12
11.5
8.5
8
10
19.5
23.5
19
(1 m)
22
24
21.5
21.5
22.5
21.5
21
21. 5
21.5
21
22
21.5
22
22.5
24
12
22
22
23
22
22
20.5
22
21.5
23.5
21
22.5
23
21.5
22
23
23.5
21.5
(5 m) (surf.)
24.5
25
25
24
24
23
24
25
24
25
25
24
24
24
24
24
25
25
24
26
23.5
24
24.5
24
24.5
24
24
24
24
24
5.5
5.4
5
5.5
7.7
6.3
5.8
5
4.8
4.8
6.2
5.7
7
6.5
7.4
7.5
6.8
6.9
7.2
7.5
6.8
7.6
7.3
6.7
6.8
6.8
6.9
6.8
6.7
7.5
7.8
7.5
7.2
(1 m)
6.6
6.5
5.4
5.7
7.4
7
5.9
6.8
5.1
6.2
6.8
5.7
6.4
6.5
6.2
6.6
5.8
5.4
5.4
6.3
7.4
7.3
6.6
6.5
5.6
6.3
5.8
6.4
6.5
6.6
8.2
7.7
7.4
(5 m)
9
8.8
6.8
8.1
7.7
7.8
7.8
8
7
7.7
8.7
7.5
7.2
4.3
6.6
5.1
6.4
6.2
9.1
6.2
7.4
7.7
7.4
6.4
7
7
7
9.4
10
9.4
(10 m)
9.7
7.7
7.5
8.2
8.6
8.3
7.3
6.5
5.5
5.4
5.6
6.4
10.3
7.9
8.4
5.6
7.7
7.7
7.4
10.8
11.2
10.6
-------
TEMPERATURE
SALINITY
DISSOLVED OXYGEN
MONTH
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
0 8
1 8
NJ n
Ch 8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
DAY YEAR HOUR MINUTE STATION DEPTH
8
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
10
10
10
10
10
10
10
10
10
10
10
10
10
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
3
4
4
5
3
3
3
3
3
3
3
3
3
3
4
4
5
5
4
5
3
4
3
4
4
2
3
3
3
3
3
3
2
45
30
35
5
0
10
15
25
30
35
40
45
50
55
15
40
10
20
45
30
40
10
45
35
50
55
, o
10
15
20
25
50
45
42
22
23
12
51
50
41
42
43
35
34
33
32
31
21
25
13
14
24
15
21
34
31
41
50
12
13
25
24
23
22
32
11
999
32
54
19
999
999
999
999
0
28
999
999
999
25
32
999
6
8
44
4
32
100
27
100
39
999
11
45
36
999
999
100
29
TIDE (surf.)
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
slack
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
outgoing
15
14
14
13.5
13.5
14
14.5
14
15
14
14
14
14
15
13
13.5
14
13.5
14
14
15.5
14.5
15.5
16.5
15
14
14.5
14.5
15
15
15
16
14
(1 m)
13.5
13.5
13.5
13 .5
13.5
13 . 5
14
14.5
14
14
13.5
14
13 .5
13
13
13.5
13
13
13.5
14
14
14
14.5
14
14
14
14
14
14
14
14
13.5
(5 m)
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13
13.5
(10 m)
13
12.5
12.5
12.5
12.5
12.5
13
13
13
13
12.5
13
12.5
13
12.5
13
13
13
12.5
13
13
13
13
13
12
(surf.)
11
9
9
6
24
21.5
13
13 .5
20
11
12.5
11
10.5
10.5
10
11.5
8
11
10
1
13
17
21
15
23
5
11
12
16.5
12
15
20
5.5
(1 m)
22
13
22
15
24.5
22.5
22
22.5
22.5
22. 5
23
23
22.5
23
22
23
19
19
21.5
20
21
23
22
24
22
22
17
22
22
21.5
21.5
19
(5 m) (surf. )
24.5
25
24
25
25
25
25
24.5
25
25
24.5
25
25
25
25
24
24.5
25
24
24.5
24
25
24
24
24
24
24.5
24
22
7.4
7 .8
8.2
8.7
8
8.2
7.5
7.6
7.4
7.8
7.5
7.8
8.2
7.4
7.7
7.2
7.7
7.5
7.8
9.2
7.4
6.5
6.5
6.2
7.8
8.9
8.3
7
6.4
7.2
7.5
6.6
7.2
(1 m)
8
6.8
6.4
7.5
8.2
8.4
6.8
6.6
6.8
6.7
7.4
6.5
6.9
6.6
6
6.2
6.7
7
6.4
6.7
6
6.8
6
7.8
6.4
6.4
6.6
6.2
6.8
6.9
6.5
6.8
(5 m)
9.8
6.6
6.3
6.2
8.2
8.2
7.2
7.5
7.6
7.5
7.4
7.5
7.3
6.7
6.4
6.5
6.4
6.7
7.2
7
7.3
7.7
6.8
7.4
7.2
7.3
6.4
6.8
6.4
(10 m)
10.6
5.4
7
8.4
7.9
7.8
8.1
8
6.5
7.6
7.2
5.5
4.9
6.2
6.8
5
7.5
6.5
7.4
7.4
7.4
7
5.9
6.6
0.75
-------
TEMPERATURE SALINITY DISSOLVED OXYGEN
MONTH DAY YEAR HOUR MINUTE STATION DEPTH TIDE (surf.) (1 m) (5 m) (10 m) (surf.) (1 m) (5 m) (surf.) (1 m) (5 m) (10 m)
8 10 88 4 25 43 999 outgoing 15.5 15 13 13 17 21 24 5.2 5.4 7.2 6.8
8 10 88 4 0 33 999 outgoing 15 13.5 13 13 15 23 24 5.4 5.8 6.8 7
8 10 88 5 0 51 100 outgoing 15 14.5 13 12.5 22 22 24 5 7 7.4 7.7
8 10 88 4 15 35 31 outgoing 15 14 13 20.5 22 24 5.2 5.5 6.8
8 10 88 4 25 42 91 outgoing 16 15 13 13 21 22.5 24 6 6.2 7.4 7.6
8 20 88 1 35 42 999 incoming 15.5 15 14 12.5 23 23 25 6.5 6.6 8.4 9
8 20 88 3 20 32 999 incoming 16 14.5 13.5 12.5 22 23 25 5.8 5.5 6.4 5.7
8 20 88 3 45 45 33 incoming 16 15 14 13 20.5 23.5 25 4.5 6 6.2 6
8 20 88 2 15 13 8 incoming 15 14 23 24 6.2 4.9
8 20 88 2 40 23 59 incoming 15 14 14 13 21.5 24 25 5 6 7.2 6.3
8 20 88 2 50 22 35 incoming 15.5 14 13.5 13 18 23.5 24.5 5.8 5.6 7 6.3
8 20 88 3 0 21 38 incoming 15 14 14 13 20 24 25 5.3 5.7 6.6 5.3
8 20 88 1 30 41 80 incoming 15 15 13.5 12 23 23.5 25.5 5.8 5.6 9 8.5
8 20 88 2 10 12 18 incoming 15 14 13 21.5 24 25.5 5.5 5.2 6.2
8 20 88 1 0 51 999 incoming 15 14.5 14 13 24 24 25 8.2 10.2 13.2 14
O 8 20 88 2 25 25 35 incoming 15 14 14 13 22 24 24.5 6.8 5.8 7.6 7.4
1 8 20 88 3 40 44 80 incoming 15.5 15 14 12.5 22 23 25 5.6 6.1 6.4 5.9
^ 8 20 88 3 30 33 999 incoming 16 14.5 14 12.5 21 23.5 25 5 5.8 6.8 5.6
8 20 88 2 0 11 25 incoming 15 14.5 13 22 24.5 25.5 4.8 5.1 6.6
8 20 88 3 10 31 27 incoming 16.5 14.5 14 21 23.5 24 6.2 6.3 7
8 20 88 2 30 24 999 incoming 15 15 14 13 22.5 23 25 5.3 5.2 7.3 7.1
8 20 88 12 50 50 37 incoming 15 14 13.5 12.5 24 24.5 26.5 8.6 9.2 10.2 10.4
8 20 88 1 10 43 0 incoming 15.5 14.5 13 12 22.5 24 25.5 5.8 8.4 11 11
8 21 88 11 35 61 999 outgoing 15 14.5 13 12 23 23.5 26 4.4 5.5 6.9 6.1
8 21 88 4 35 50 999 incoming 15 14.5 14.5 12.5 23 23 24 8.6 9 10.1 9.1
8 21 88 4 45 51 999 incoming 14 14 14 13 23.5 23.5 25 9.7 9.8 9.7 9.4
8 21 88 12 ,30 65 150 outgoing 14.5 14.5 13.5 12 23 23.5 26 6.6 6.4 6.3 6.3
8 21 88 11 45 62 999 outgoing 14.5 14.5 13 12 24 24 26 6.5 6.5 6.9 7.9
8 21 88 3 40 22 999 incoming 15 15 14 12.5 22.5 22.5 24 6.5 6.1 7.7 6.9
8 21 88 4 0 23 999 incoming 15.5 15 14 12.5 21 23 24 6.8 6.8 7.5 8.2
8 21 88 6 25 25 39 incoming 16 15.5 14 12.5 21 22 25 5 5.3 6.7 6.5
8 21 88 7 15 11 36 slack 15 15 13 12 17 23 25 5.7 5.7 6.2 0.5
8 21 88 12 5 63 999 outgoing 14.5 14.5 14 12 23.5 23.5 25 6.6 6.1 7.1 7
-------
TEMPERATURE SALINITY DISSOLVED OXYGEN
MONTH DAY YEAR HOUR MINUTE STATION DEPTH TIDE (surf.) (1 m) (5 m) (10 m) (surf.) (1 m) (5 m) (surf.) (1 m) (5 m) (10 m)
8 21 88 12 20 64 999 outgoing 14.5 14.5 13.5 12 23.5 23.5 26 7 6.4 6.6 6.3
8 21 88 3 0 35 25 incoming 15.5 15 14.5 22.5 23 23.5 4.5 5 6
8 21 88 5 0 43 999 incoming 14.5 14.5 14 13 23.5 23.5 24 8.4 9.2 8.5 8.1
8 21 88 5 10 42 999 incoming 15.5 14.5 14 13 22 23.5 25 6.4 9.4 8.4 8.5
8 21 88 5 45 41 999 incoming 15.5 15 13.5 12.5 22.5 22.5 25 5.7 6.4 8.3 7.1
8 21 88 5 35 32 999 incoming 16 15.5 14 12.5 22 22 24 6.2 5.7 7.2 6.6
8 21 88 5 20 31 30 incoming 16 15 14 13 22 23 24.5 5.7 5.7 8 5.7
8 21 88 7 45 12 31 slack 15.5 15.5 13.5 7 20 23 8.8 5.2 6
8 21 88 5 45 33 999 incoming 15.5 15 14.5 12.5 22 23.5 24 6.7 5.8 7.8 6.7
8 21 88 3 20 24 40 incoming 15.5 15 14 12.5 21 22.5 24 7.1 6.6 7.9 7.5
8 21 88 8 0 13 999 outgoing 15.5 15 20.5 23 5.4 5.3
8 22 88 3 45 25 34 incoming 16 15 14 13 22 24.5 26 4.3 5 6.4 7
8 22 88 5 15 50 26 incoming 15 15 14 13.5 25 25 26 7.4 7.6 7.8 7.2
8 22 88 4 40 35 999 incoming 17 15.5 14 21.5 24 25.5 5.1 4.6 6.3
8 22 88 4 50 43 80 incoming 16 15 14 13 24 24.5 25.5 5.1 5.5 7.6 7
I 8 22 88 4 30 33 999 incoming 16 16 14 13 23 23.5 26 4.8 5.2 7.2 7.2
NJ 8 22 88 4 35 34 999 incoming 16.5 15 14 13 20.5 24 26 5.2 5.4 6.8 7.6
°° 8 22 88 4 10 22 999 incoming 15.5 15 14 13 22.5 24 26 4.9 4.4 6.2 6.4
8 22 88 5 40 51 999 incoming 15 15 14 13 25 24.5 26 8 7.8 8.1 7.6
8 22 88 5 30 71 999 incoming 14.5 14.5 14 13 25.5 26 26.5 8.2 8 8 7.8
8 22 88 3 35 13 7 incoming 16 15 20 24 4.5 4.3
8 22 88 4 0 23 999 incoming 16 15 14 13 22 24 26 5.2 4.6 7.1 8
8 22 88 3 55 24 999 incoming 16 15 14 13 22 24.5 26 5.4 5 6.6 7.4
8 22 88 3 30 12 25 incoming 15.5 15 14 10 23.5 25.5 5.6 5.5 4.8
8 22 88 4 5 21 0 incoming 15 14.5 14 13 23 24 26 4.6 4.3 5.8 6.2
8 22 88 5 0 42 999 incoming 16 15.5 14 13 23.5 24 26 5.3 6.2 7.8 7.2
8 22 88 4 20 32 999 incoming 16 15.5 14 13 24 24 26 5.5 5 6.7 7.2
8 22 88 5 ' 5 41 80 incoming 15.5 15 14 13 24 24.5 25.5 4.5 5 6.8 6.4
8 22 88 3 20 11 23 incoming 15.5 15 13 6 24 29.5 8 5.6 4.7
8 23 88 4 45 12 15 incoming 16 16 22.5 25 9 6.1
8 23 88 7 20 42 999 incoming 16 16 14 13.5 24 25 26 6.4 5.8 7.4 8.6
8 23 88 6 5 33 999 incoming 17 16 14 13 21 25 26.5 6.6 5.2 8 7.8
8 23 88 6 0 32 80 incoming 17 16 14 13 23 25 27 5.4 5 8.9 8.5
-------
TEMPERATURE SALINITY DISSOLVED OXYGEN
o
KJ
ITH DAY YEAR HOUR MINUTE STATION DEPTH
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
9
9
9
23
23
23
23
23
23
23
23
23
23
23
23
23
24
24
24
24
24
24
24
24
24
24
24
13
13
13
13
13
13
13
13
13
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
5
6
5
4
4
5
6
5
6
5
5
7
7
10
11
11
11
11
11
10
11
10
11
11
11
12
12
12
12
1
12
11
11
20
45
45
35
50
0
10
15
55
35
10
10
20
45
20
40
50
55
30
35
5
55
5
25
20
0
,30
45
45
0
40
10
35
22
34
31
11
13
25
11
23
35
21
24
43
41
71
13
23
22
21
24
50
11
51
12
25
51
31
35
23
24
25
22
71
42
33
80
15
25
6
34
27
60
31
33
999
999
80
80
12
999
39
999
39
33
31
80
31
39
999
28
32
999
999
39
37
999
999
TIDE (surf.)
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
slack
slack
outgoing
slack
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
16
17
17.5
16
16
17
16
16
16.5
16
16
16.5
16
15
16
15.5
16
16
16
15.5
16
15
16
16
12.5
14
13.5
13
13
13.5
14
12.5
13
(1 m)
15.5
16
16
16
15.5
15.5
15.5
15.5
16.5
15
16
16
16
14.5
15
15
15
15
16
14
15
14.5
15
16
12.5
13.5
13
13
13
12.5
12.5
12
12.5
(5 m)
13.5
14.5
13
14
14
13.5
14.5
13.5
13.5
14.5
14
14
13
13
13
13
13.5
13.5
14
13.5
13.5
12
12
12.5
12.5
12.5
12.5
12.5
12
12
(10 m)
12
13
12.5
12
13
12
12.5
13.5
13
13
12.5
12
12
12
12.5
12
13
12
12.5
12
12
12.5
12
12
12
12
12
(surf.)
23.5
22
22
5
24
23
7
10
24.5
23
20
24
25
25
14
24
24
24
24
25
20.5
25
21.5
23
27
17
23
22
24
22
16
27
26.5
(1 m)
24
25
24
25
25
25
25
25
24.5
24.5
25
25
24.5
26
25
26
25
24
24
25.5
25
25.5
25
23.5
27
22
27
22
25
26
27
27
27
(5 m) (surf.)
27.5
27
28.5
27
26
27
26
27
27.5
26
26
26.5
28
28
28
28
27
27.5
26.5
25
27.5
28.5
29
28
27
28
27.5
28
27
27.5
6
5.2
6
7.3
6.7
5.4
7.7
5.1
6.2
5.6
7.1
7
4.7
8.7
6.9
6.1
5.8
5.4
5.8
6.4
6.6
8.2
7.2
5.5
7.5
6.5
6. 1
5.8
5.6
7
6.5
7.9
6.7
(1 m)
4.5
6
5.5
5
5.1
6
6.3
5
5.3
5.2
6.1
6
4.5
8.7
5.4
6.2
6
5.3
5.8
6.7
7
8.2
6.1
5.7
7.4
6.1
6.5
6
5.6
5.9
6.1
7.8
7.1
(5 m) (10 m)
8.2
9.5
6.5
7.2
7.1
7.2
7.8
7.7
7.5
8.6
7.6
8.4
5.6
5.3
4.7
6
7.9
6.1
8.2
5.2
6
7.2
6.3
6.5
6.2
6.1
6.4
5.9
7.6
7.2
5.2
9.2
6.2
6.5
8.1
6.4
7
8.7
8.2
8.1
5.7
4.5
4.2
6
7.8
0.5
7.8
0.5
5.6
7.3
5.9
6.3
5.7
6.1
4.6
7.6
6.4
-------
MONTH
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
0 9
1 9
OJ
0 9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
DAY YEAR HOUR MINUTE STATION DEPTH
13
13
13
13
13
13
13
13
13
13
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
14
16
16
16
16
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
11
10
11
1
12
12
1
12
12
12
12
12
12
1
1
1
2
1
1
2
12
12
12
1
1
12
1
12
12
10
12
12
12
30
50
40
20
10
15
10
35
15
20
0
35
55
30
35
55
0
10
20
10
40
20
30
45
5
45
50
'10
50
17
45
2
15
43
51
41
11
32
33
13
21
12
34
50
42
41
22
23
13
12
31
21
11
35
51
43
24
32
34
25
71
33
12
43
32
33
999
999
80
35
999
999
14
40
999
999
43
80
80
40
63
14
30
39
42
34
32
999
80
43
80
80
40
999
80
22
999
80
80
TIDE (surf.)
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
12.5
13
14
13.5
14.5
14
13
14
13
13.5
13.5
15
14
14
14
14
13.5
14
13.5
13.5
14
13
14
14.5
15
14
14
13
14
12
13
14
14.5
TEMPE]
(1 m)
12.5
13
13.5
12.5
14
13
12.5
13
13
13
13
13.5
13.5
13
13.5
13
13
13
13
13
13
12.5
13.5
13.5
13
13.5
13
13
13.5
12.5
13
13
14
MATURE
(5 m)
12
12.5
12
12.5
12.5
12.5
12.5
12
12.5
12.5
12.5
12.5
13
13
13
12.5
12.5
13
12.5
12.5
12.5
12.5
12.5
13
12.5
12.5
12.5
12
12.5
12
12
(10 m)
12
12
12
12
12
12
12
12
12
12
12
12
12
12.5
12
12
12
12.5
12
12
12
12.5
12.5
12
12
12.5
12
12
12
Si
(surf.)
27
22
21
4
19
23
9
15.5
23
23
25
19.5
24
23
16
23
24
21
21.5
23
21
26
22 .5
20
18
22
22
25
22
11
24
25
24
MjINlTY
(1 m)
27
23.5
25
26
23
25
27
25
26
27
25.5
23
25
25
24.5
25
25.5
24
25
25
23
26.5
24
24
25
23
25
25
22.5
26
24
25.5
25
UJ.&
(5 m) (surf. )
27.5
23
28
27
27.5
28.5
28
27.5
28
28
24.5
27
25.5
26
26.5
27
26.5
26
23
27
26
27
27
24
27
27.5
24
28
26
28
27
6.3
8.3
5.4
8.1
6.2
5.8
8.3
6.5
6.8
5.6
8.1
6.4
6.2
7.1
8.8
9.1
8.9
6.5
6.4
7.8
7.2
7.7
7.9
7.2
7.1
7. 1
7.3
8.7
7.5
9. 6
8.1
7
6.8
it>UJjV£,U
(1 m)
6.9
6.9
4.8
5.6
5.6
5.8
5.9
5.8
5.8
6.2
7.9
7
6.3
7.1
7
8.4
7.8
6.3
6.5
7.6
7.6
7.4
7.7
7.9
6.8
7.7
7.8
8.3
7.8
7.6
8.1
7.5
6.8
uAHjr,r<
(5 m) (10 m)
6.9
7.4
6.4
5.7
6.3
6.3
5.7
5.6
6.6
7.4
7.4
6.7
7.1
7.7
7.4
6.8
6.5
7.6
7.3
7.2
7.2
7.4
7.2
7.4
7.5
7.5
7.4
6
8.5
7
6.5
6.5
7.2
5.9
0.5
6.2
6.1
4.7
0.5
6.4
6.7
7.3
6.3
6.1
6.9
6.2
6.5
5.2
7.2
7.3
7.4
7
7.2
7.2
7.1
7.6
6.5
7.5
7
6.5
-------
TEMPERATURE
MONTH
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
„ 9
? 9
W 9
H> 9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
DAY YEAR HOUR MINUTE STATION DEPTH
16
16
16
16
16
16
16
16
16
16
16
16
16
16
16
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
17
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
88
11
11
1
10
11
10
1
11
12
1
10
1
12
12
10
1
12
11
2
12
11
12
1
11
12
1
1
2
11
1
1
11
11
0
40
35
25
28
10
4
13
22
19
40
46
32
55
50
25
20
30
0
30
50
5
15
40
40
50
40
'15
10
5
35
20
55
23
31
71
11
22
13
41
21
34
50
25
51
35
42
24
43
31
25
71
32
23
22
35
24
33
50
41
51
11
34
42
12
21
60
24
90
32
24
3
80
32
999
999
999
999
24
999
44
999
999
999
999
999
999
999
999
999
999
999
999
999
999
999
999
999
999
TIDE (surf.)
incoming
incoming
incoming
incoming
incoming
slack-low
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
incoming
13
15
13
14.5
12.5
12.5
13 .5
13
14.5
13
12.5
13
13.5
13 .5
13
13.5
15
12.5
12.5
14
13
100
14.5
13
14.5
13
14
13
12.5
14
14 .5
13
14
(1 m)
13
13
13
14.5
12.5
12.5
13
12.5
14
13
12
13
14
13
13
13
13
13
12.5
13
13
13
14
13
13
13
13
13
13
13
13
13
13
(5 m)
12
12
12
13
12
12
12
12
12.5
12
13
12.5
12.5
12
12.5
12
12
12.5
12
12
12
12.5
12
12
12.5
12
13
12
12
12.5
12
12
(10 m)
12
12
12
12
12
12
12
12
12.5
12
12
12
12
12
12
12
12
12
12
12
12
12
11.5
SALINITY
(surf.)
22.5
24
26
17.5
25
12
25
25
23
26
13
25.5
22.5
24
14.5
24.5
22
12
25.5
24
15.5
22.5
12
23
25
24
25
6
24
23.5
7
22
(1 m)
24.5
26
26
23.5
26
25.5
25. 5
26
24.5
26
26
25.5
23
25
25
25
24.5
25
25.5
25
24
24.5
23.5
25
24.5
25
25
25
25
25
25
25.5
25
DISSOLVED OXYGEN
(5 m) (surf.)
28
28
27.5
25
28
27
27.5
27.5
26.5
28
26
27
26
27.5
26
27
27
26. 5
27.5
27
27
26.5
27
27
26
27
25
27
27
26
27
27
7.5
7.6
9.6
9.6
6.5
9.4
7
6.4
7.1
9.1
9.2
9.2
7.5
7.8
9.3
7.7
7.6
9.4
7.6
7
8.7
6.9
10.2
7.4
8.3
5.4
7.5
9.2
6.1
7.2
9.2
7
(1 m)
7.1
7.4
9.6
6.6
6.3
7.3
7.6
6.4
6.7
8.8
6.2
9.4
7.2
7.8
7.5
7.4
6.8
7
7.5
7.4
6.7
6.8
6.5
7
6.4
8.3
. 6.3
7.6
6.7
6.6
6.8
6
6.5
(5 m) (10 m)
7.3
6.5
8.4
7
6
8
5.8
6.4
8.8
7.3
9.4
7.8
8.4
7.6
8.4
7.4
7
7.2
6.6
6.3
5.5
6.7
7.4
6.5
8.1
6.1
8
5.4
6.7
8.4
4.2
6.8
5
8.4
3.8
7.3
4
6.2
7.8
8.2
8.2
6.6
7.4
5.8
7.3
4.9
5.8
6.6
6.8
6.4
6.6
7
6.4
8.9
1.5
-------
APPENDIX D
BIOASSAY RESULTS
-------
TOXSCAN, INC.
-------
MARINE BIOASSAY LABORATORIES
1234 HIGHWAY ONE
WATSONVILLE, CA 95076
(408) 724-4522
PRELIMINARY ASSESSMENT OF THE EFFECTS OF TEMPERATURE AND
DISSOLVED OXYGEN UPON THE TOXICITY OF PULP MILL EFFLUENT TO
JUVENILE COHO SALMON (ONCORHYNCHUS KISUTCH)
Prepared for:
JONES AND STOKES ASSOCIATES, INC.
Bellevue, Washington
Prepared by:
TOXSCAN, INC.
MARINE BIOASSAY LABORATORIES DIVISION
Watsonville, California
DECEMBER, 1988
MBL
A DIVISION OF TOXSCAN, INC.
D-l
-------
Introduction
As part of Work Assignment No. 12 under EPA Contract No. 68-02-
4381 between EPA Region X and Jones and Stokes Associates, Inc.,
Kinnetic Laboratories, Inc., through it's affiliated firm ToxScan,
Inc., has performed toxicity studies using juvenile Coho Salmon
(Oncorhynchus kisutch). The studies were designed to yield preliminary
data on the relationship between sublethal stress (low dissolved oxygen
and high temperature) and observed toxicity of effluent from a pulp
mill. These salmonid tests represent one portion of a multi-species
toxicity testing program which, together with oceanographic and field
biology studies, comprised an investigation of repeated fish kills
observed in Ward Cove near Ketchikan, Alaska.
Background
Repeated fish kills have been observed in Ward cove, with affected
species including adult coho and pink salmon, adult herring and
juvenile salmonids. Most fish kills seem to occur during August and
September. Ward Cove is the site of a pulp mill owned by Ketchikan
Pulp company and a salmon processing facility owned by Ward Cove
Packing Company. It seems possible that fish kills may be related to
the operations of these two industries.
Historical data indicate that low levels of dissolved oxygen occur
regularly during summer within the top and bottom stratified layers of
Ward Cove water, and Kruse and Viteri (1988) hypothesize that fish
kills may be caused by a combination of low dissolved oxygen in concert
with toxicants in the pulp mill effluent. This study was designed to
investigate the relationship between dissolved oxygen, temperature and
effluent toxicity in affecting fish mortality.
Experimental Design
Juvenile coho salmon were exposed to a series of dissolved oxygen
concentrations ranging from the normal 8 ppm down to 3 ppm, to
determine an LC50 for dissolved oxygen, i.e., to quantify dissolved
oxygen levels which produced obvious stress and subsequent mortality.
Following identification of a critical dissolved oxygen concentration,
salmon were exposed to a concentration series of pulp mill effluent at
high, intermediate, and low dissolved levels to test the hypothesis
that dissolved oxygen and effluent stresses are additive (or perhaps
synergistic) in contributing to fish mortality. This series of tests
was performed at two different temperatures, e.g. at about 8°C
(corresponding to the temperature of offshore waters) and again at
about 18°C (well within the range of temperatures reported in nearshore
waters in the vicinity of Ward Cove).
D-2
-------
Methods
Juvenile coho salmon (Oncorhynchus kisutch) were purchased from
Silverking Oceanic Farms, Inc., Santa Cruz, California. All fish were
held in laboratory tanks for at least 2 weeks prior to testing.
Acclimation temperature was 12°C, and fish were fed commercial trout
chow pellets. Less than 2% mortality was observed during the
acclimation period, and all fish appeared healthy and vigorous. Size
range was 4-6 cm (0.4-0.6 g), and individuals were randomly withdrawn
from acclimated laboratory populations for assignment to test tanks.
Attempts to acclimate the juveniles to increased salinity resulted
in elevated mortality, so testing was done in freshwater.
Test tanks were 10-liter polycarbonate which had been cleaned
according to protocol-prescribed methods, including acid and acetone
rinses to remove adsorbed inorganic and organic contaminants. Dilution
and control water was dechlorinated municipal water, which was also
used as acclimation water for the fish.
Dissolved oxygen concentration in test ranks was controlled by
bubbling nitrogen and air simultaneously into each tank. The final
D.O. depended upon the rate of addition of each gas, and the
empirically-determined rates were controlled by valved precision flow-
meters (Cole-Parmer Model J-3216). Test temperatures were achieved in
two ways: for the 18°C test, room temperature was maintained by an air
conditioner linked to a precision mercury thermoregulator. A large fan
in the test room maintained air circulation adequate to prevent
vertical stratification. For the 8°C test, tanks were placed inside a
large commercial refrigerator modified to hold test temperature within
the specified +_ 1°C limits. Photoperiod was 16 hours light:8 hours
dark. Fish were not fed during testing. One week prior to initiation
of testing at 8°C and 18°C, fish were slowly brought to the appropriate
test temperature from their 12°C laboratory acclimation temperature.
Transition from acclimation to test temperature was done over two days,
and fish were held at the test temperature for five days before testing
began.
Test protocols generally followed the guidelines in EPA 600/4-
85/013, Methods for Measuring the Acute Toxicity of Effluents to
Freshwater and Marine Organisms.
Effluent Sampling
Grab samples of effluent were collected from three outfalls at the
pulp mill, designated main, primary and secondary, on 20 September,
1988. Samples were collected into 5 gallon collapsible plastic carboys
which were immediately placed in iced coolers for air shipment to the
ToxScan laboratory in Watsonville, CA. Samples arrived on 21 September
D-3
-------
and were placed in the laboratory 4°C cold room where they remained
until testing was initiated. Following EPA Region X instructions, the
three effluent samples were mixed in the proportions 38.5% main: 45%
primary: 16.5% secondary. The resulting effluent composite was used as
the test material for these experiments.
Results
The dissolved oxygen LCcQ test was performed on 12-16 September,
and the results are summarized in Table 1. Control of D.O. levels in
test tanks was reasonably good, but required frequent monitoring and
some compensatory adjustment of gas flow rates. Monitoring of D.O. is
reported as the mean of hourly measurements taken each day. pH was
measured daily. Temperature data are not reported, but all were +_
0.4°C of the target level. The LCjQ for dissolved oxygen was 4.65
mg/1, with 95% confidence limits on those data of 4.55 mg/1 and 4.76
mg/1.
The 18°C effluent test was done on 26-30 September (Tables 2, 4
and 6). The mean dissolved oxygen level in the low D.O. test was 4.72
mg/1, in the medium D.O. test the mean was 6.53 mg/1 and in the high
D.O. test the mean was 8.44 mg/1. The LCcQ for pulp mill effluent was
32% (confidence limits 28.4 and 35.8%), in low D.O., 28.1% (25.0 and
31.6%) in medium D.O. and 42.1% (0, infinity) in high D.O.
The 8°C effluent test was done on 3-7 October, (Tables 3, 5 and
7). Mean dissolved oxygen levels in low, medium and high D.O. tests
were, respectively, 4.92 mg/1, 6.63 mg/1 and 10.73 mg/1. Effluent
LC5Qs were 35.8% (27.1 and 47.7%) in low D.O., 35.1% (27.9 and 44.2%)
in medium D.O., and 44.5% (36.7 and 49.1%) in high D.O.
r
The results are tabulated in the text table below, and are
expressed both in terms of LC50 (* effluent) and in toxicity limits
(t.u.'s), calculated by dividing 100 by the LC50 in percent (100/LC50).
Pulp Mill Effluent Toxicity
Low D.O. Medium D.O. High P.O.
LCSO - 18°C 32.0% 28.1% 42.1%
8°C 35.8% 35.1% 44.5%
t.u. - 18°C 3.13 3.56 _ 2.38
8°C 2.79 2.85 2.25
D-4
-------
Discussion
The results of this very preliminary study suggest the following
conclusions.
- At each dissolved oxygen level tested, the toxicity of the pulp
mill effluent is lower at 8°C than at 18°C.
- At either experimental temperature, the mill effluent is more
toxic at very low dissolved oxygen than at high dissolved oxygen.
- The toxicity tests done at intermediate levels of dissolved oxygen
show no reduction of toxicity when compared with tests done at low
D.O. levels. Toxicity at 18°C, in fact, seems slightly increased
with a moderate increase in D.O.
Without replication of toxicity tests, no statistical evaluation
of these data can be done. A crude approach to quantification of the
comparison among tests can be made by expressing the toxicity reduction
in terms of percent of the higher toxicity. The calculation was done
according to the formula:
higher toxicity - lower toxicity
% reduction = — X 100
higher toxicity
For the 18°C data, toxicity at high D.O. is 24% less than toxicity
at low D.O.; for the 8°C data the corresponding reduction in toxicity
is 19.4%.
For the low D.O. data, toxicity is reduced by about 11% at 8°
compared with that at 18°C; for the medium D.O. data the toxicity
reduction is about 20%, and for the high D.O. data the toxicity is
reduced by about 6% with a 10°C temperature decrease.
If the intuitively worst-case situation (low D.O., high
temperature) is compared with the best-case situation (high D.O., low
temperature), the toxicity reduction is about 28%.
These results tend to support the hypothesis that the adverse
environmental conditions observed in Ward Cove during the summer months
can potentiate the toxic effects of pulp mill effluent.
D-5
-------
TABLE 1
DISSOLVED OXYGEN - 12°C
Mean Dissolved Oxygen
Tank # Concentration (mg/1)
1 3.05
2 4.20
3 4.62
4 5.48
5 6.30
6 7.24
7 8.60
Number of Survivors
0 hrs 24 hrs 48 hrs 72 hrs
96 hrs
10
10
10
10
10
10
10
0
1
8
10
10
10
10
0
1
7
10
10
10
10
0
1
7
10
10
10
10
0
0
7
9
10
10
10
LC50 (D.O.) = 4.65 mg/1
95% confidence limits = 4.55 mg/1, 4.76 mg/1
Tank
1
2
3
4
5
6
7
Tank
1
2
3
4
5
6
7
Dissolved Oxygen (mg/1)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
3.3
4.3
4.6
5.1
6.4
7.1
8.8
0 hrs
8.4
8.4
8.4
8.3
8.3
8.4
8.4
3.8
3.5 4.1
4.7 4.7
5.8
6.5
7.0
8.5
pH value
24 hrs 48
8.3
8.3
8.3
8.4
8.3
8.4
8.3
5.5
6.2
7.4
8.6
(pH
hrs
8.3
8.2
8.3
8.3
8.2
8.3
8.3
3.9
4.5
5.6
6.3
7.3
8.7
units)
72 hrs
8.3
8.2
8.2
8.2
8.2
8.2
8-. 3
3.8
4.6
5.4
6.1
7.4
8.4
96 hrs
8.2
8.1
8.2
8.1
8.2
8.2
8.2
D-6
-------
TABLE 2
PULP MILL EFFLUENT - 18°C
Low Dissolved Oxygen (Mean = 4.72 mg/1)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Number of Survivors
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
10
10
10
10
10
10
0
101
10
10
10
10
0
82
10
10
10
10
0
72
10
10
10
10
0
52
10
10
10
10
LC50 = 32%
95% confidence Hmits = 28.4%, 35.8%
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Dissolved Oxygen (mg/1)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
4.6
4.8
4.7
4.9
4.4
4.8
4.7
4.7
4.6
4.1
4.3
4.6
_
5.2
4.9
4.9
4.8
4.1
.
4.4
5.1
5.0
4.8
4.3
_
4.8
5.0
4.8
4.8
4.8
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
0 hrs
pH Value (pH units)
24 hrs 48 hrs 72 hrs 96 hrs
7.8
7.9
8.0
8.2
8.3
8.4
8.1
8.1
8.1
8.1
8.2
8.3
7.8
8.0
8.0
8.2
8.3
8.0
8.1
8.1
8.3
8.3
.
8.0
8.0
8.1
8.2
8.3
Behavioral observations
1. None Swimming; all twitching on bottom of tank
2. About 50% of fish swimming, all twitching
D-7
-------
TABLE 3
PULP MILL EFFLUENT - 8°C
Low Dissolved Oxygen (Mean = 4.92 mg/1)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
0 hrs
Number of Survivors
24 hrs 48 hrs 72 hrs 96 hrs
10
10
10
10
10
10
0
101'2
10
10
9
10
0
93
9
9
10
0
81
8
9
9
10
0
71
8
8
9
9
LC
50
= 35.1
95% confidence limits = 27.1%, 47.7%
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Dissolved Oxygen (mg/1)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
4.9
4.8
5.0
4.6
5.0
4.9
0 hrs
8.2
8.4
8.6
8.5
8.6
8.7
4.7
4.6
4.8
4.9
5.1
5.0
pH Value
24 hrs 48
8.3
8.4
8.5
8.6
8.6
8.5
4.7
5.0
4.9
5.0
5.2
(pH
hrs
_
8.3
8.5
8.4
8.5
8.6
4.9
5.1
5.1
4.8
5.3
units)
72 hrs
_
8.3
8.5
8.7
8.7
8.7
_
5.0
4.9
5.1
4.9
4.8
96 hrs
_
8.4
8.4
8.6
8.6
8.7
Behavioral observations
1. Activity level much reduced; slow swimming with a "rolling" motion
2. All twitching
3. 2 (of 9) twitching
D-l
-------
TABLE 4
PULP MILL EFFLUENT - 18°C
Medium Dissolved Oxygen (Mean = 6.53 mg/1)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Number of Survivors
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
10
10
10
10
10
10
10
101
10
10
10
10
0
8
9
10
10
10
0
7
9
10
10
10
0
5
8
10
10
10
LCcg = 28.1%
95% confidence limits = 25.0%, 31.6%
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Dissolved Oxygen (mg/1)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
6.2
6.3
6.4
6.4
6.3
6.2
0 hrs
7.9
7.9
8.1
8.1
8.2
8.4
7.2
6.7
6.8
6.2
6.5
6.8
pH Value
24 hrs 48
8.2
8.1
8.1
8.2
8.2
8.3
_
6.5
6.7
6.9
6.7
6.7
(pH
hrs
_
7.8
8.0
8.1
8.1
8.2
af
6.3
6.5
6.4
6.6
6.4
units)
72 hrs
_
8.0
8.0
8.1
8.2
8.2
—
6.6
6.8
6.3
6.4
6.4
96 hrs
__
8.0
8.1
8.1
8.1
8.1
Behavioral observations
1. Eratic, darting swimming behavior, all twitching,
D-9
-------
TABLE 5
PULP MILL EFFLUENT - 8°C
Medium Dissolved Oxygen (Mean = 6.63 mg/1)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Number of Survivors
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
10
10
10
10
10
10
0
101
10
10
10
10
0
102
93
10
9
9
0
82
8
10
9
9
0
72
8
9
9
9
LC
50
35.1%
95% confidence limits = 27.9%, 44.2%
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Dissolved Oxygen (mg/1)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
6.7
6.8
6.9
6.8
6.8
6.7
7.2
7.1
6.9
7.0
6.9
6.6
7.0
7.0
6.5
6.9
7.0
_
6.7
6.6
6.5
7.0
6.7
_
6.9
6.9
6.7
6.8
6.0
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
0 hrs
pH Value (pH units)
24 hrs 48 hrs 72 hrs 96 hrs
8.2
8.4
8.6
8.6
8.6
8.6
8.4
8.4
8.5
8.6
8.6
8.5
8.4
8.4
8.5
8.4
8.5
_
8.4
8.5
8.6
8.5
8.6
_
8.4
8.4
8.6
8.6
8.6
Behavioral observations
1. Activity level very low; fish quiet
2. All fish twitching
3. 2 of 9 twitching
D-10
-------
TABLE 6
PULP MILL EFFLUENT - 18°C
High Dissolved Oxygen (Mean = 8.44 mg/1)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Number of Survivors
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
10
10
10
10
10
10
0
101
10
10
10
10
0
101
10
10
10
10
0
101
10
10
10
10
0
102
10
10
10
10
LC5p = 42.1%
95% confidence limits = 0, infinity
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Dissolved Oxygen (mg/1)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
8.2
8.0
8.0
8.7
8.6
9.1
8.3
8.9
8.0
8.7
8.4
8.5
_
8.8
8.7
9.2
8.9
9.1
_
8.5
8.2
8.7
7.5
8.4
_
8.6
8.6
8.8
6.9
7.8
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
0 hrs
pH Value (pH units)
24 hrs 48 hrs 72 hrs 96 hrs
7.9
8.0
8.0
8.2
8.2
8.4
8.1
8.1
8.0
8.2
8.1
8.3
_
8.0
8.1
8.1
8.0_
8.1
_
8.2
8.0
8.2
8.0
8.1
__
8.1
8.0
8.2
8.1
8.0
Behavioral observations
1. Slow, rolling swimming motion.
2. 8 swimming as above; 2 twitching on bottom
D-ll
-------
TABLE 7
PULP MILL EFFLUENT - 8°C
High Dissolved Oxygen (Mean = 10.73 mg/1)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Number of Survivors
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
10
10
10
10
10
10
101
102
10
10
9
10
61
92
10
9
9
10
21
93
10
9
9
10
I1
94
10
9
9
10
LC50 = 44.5%
95% confidence limits = 36.7%, 49.1%
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Effluent Concentration (%)
56
32
18
10
5.6
0 (control)
Dissolved Oxygen (mg/l)
0 hrs 24 hrs 48 hrs 72 hrs 96 hrs
10.0
9.9
10.5
10.4
10.8
10.2
0 hrs
8.3
8.4
8.6
8.5
8.6
8.6
10.8
11.0
11.0
11.0
11.2
11.2
pH Value
24 hrs 48
8.4
8.5
8.5
8.6
8.6
8.6
10.5
10.8
10.6
10.6
10.6
10.6
(pH
hrs
8.4
8.5
8.4
8.5
8.5-
8.5
10.4
10.5
10.6
10.4
10.7
10.8
units)
72 hrs
8.4
8.5
8.5
8.6
8.6
8.6
10.8
11.0
11.2
11.2
11.2
11.3
96 hrs
8.4
8.4
8.4
8.6
8.6
8.5
Behavioral observations
1. All twitching
2. All quiet, not swimming
3. 2 swimming, 7 quiet
4. All quiet, 5 twitching
D-12
-------
EPA MANCHESTER LAB
-------
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
REGION 10
SEATTLE, WASHINGTON 98101
M/S Lab November 18, 1988
MEMORANDUM
SUBJECT: Ketchican Pulp and Paper (Ward Cove) Sediment Toxicity Test Results
FROM: Joseph M. Cummins, Aquatic Bi
Laboratory Branch
TO: Duane W. Kama
Water Permits and Compliance Branch
Attached are the results of the amphipod-sediment toxicity tests conducted
in conjunction with the water quality study of Ward Cove involving the Ketchican
Pulp and Paper Company. Also included are measurements of interstitial water
salinities, gross observations of selected sediment characteristics, and a
description of the water quality conditions characterizing representative sediment
test preparations.
The sediment toxicity tests were conducted according to the amphipod-sediment
toxicity test protocol described in the "Puget Sound Protocols" (Tetra Tech, 1986)0
A few modifications in the protocol were made to adapt the test to specific
conditions of the study. These modifications are included in the summary of the
test method presented below.
rFive replicates of the test and control sediments were included in the
evaluation. Each replicate consisted of a 2-cm layer of well-mixed sediment
overlayed with approximately 800 mL of seawater (Salinity 28 o/oo). Each test
preparation was randomly seeded with twenty marine amphipods and incubated at 15°C
for ten days with aeration and continuous, fluorescent illumination. Water quality
conditions characterizing representative sediment preparations were measured on Day
0 and Day 10 of the test. The response parameters used to assess sediment toxicity
were amphipod survival, emergence, and reburial. Sediment that was originally
inhabited by the amphipods used for testing served as the Control. This sediment,
together with the amphipods, was collected from West Beach, Whidbey Island,
Washington. A "particle size" Control was not included in the evaluation because
the particle size characteristics of the test sediments from Ward Cove were unknown
prior to testing. Cadmium chloride obtained from the EPA Environmental Monitoring
and Support Laboratory, Cincinnati, Ohio was used as a reference toxicant in a
sediment-free test system.
In summary, all of the sediments collected from Ward Cove were acutely toxic
to the amphipod, R. abronius (Table 1). Amphipod survival ranged from a high of
64 % in EPA Sample No. 88390120 to a low of 7 % in EPA Sample No. 88390121. Mean
amphipod survival for all of the Ward Cove samples tested was 43 %. No mortalities
were observed in the Control, and amphipod response to the reference toxicant was
considered acceptable (96-h LC50 = 1.34 mg Cd/L; 95 % Confidence Limits 1.16 -
1.55 mg Cd/L).
D-13
-------
The mean survival of 7 % was lower than the mean survival measured in any of
the sediments collected from 48 stations occupied in 1984 during a reconnaissance
survey of eight urban and baseline bays in Puget Sound (Battelle, 1986). However,
survival values similiar to, or lower than this have been reported by Swartz et
jil_ (1982) in Commencement Bay sediments subject to a variety of chemical
contaminants and by the National Marine Fisheries Service in sediments from
Eagle Harbor containing high concentrations of creosote (Personal Communication,
Dr. Michael Schiewe, NMFS Laboratory, Mukilteo, WA, 1984).
The cause of the toxicity measured in the Ward Cove sediments cannot be
determined based on the results of this limited toxicological evaluation. All
of the water quality characteristics measured on days 0 and 10 of the test
appeared to be within acceptable limits (Table 3). Interstitial water salinities
(26 o/oo - 31 o/oo) were also considered acceptable (Table 4). However, the fine
particle size of the sediments, coupled with elevated sulfide levels and relatively
low sediment pH values could possibly have contributed to one or more of the toxic
responses observed (Table 4). Correlation of these toxic responses with all of
the physical and chemical data you and others have collected in conjunction with
this study will be needed before more definitive cause and effect relationships
can be established.
Please let me know if you have any questions.
References:
Swartz, R.C., W.A. DeBen, K.A. Sercu, and J.O. Lamberson. 1982. Sediment
toxicity and the distribution of amphipods in Commencement Bay, Washington,
U.S.A. Mar. Pollut. Bull. 13:359-364.
' Tetra Tech, Inc. 1986. Recommended protocols for measuring selected
environmental variables in Puget Sound. Final Report TC-3991-04., Tetra Tech,
Inc., Bellevue, WA.
D-14
-------
Table 1. Responses of the Marine Amphipod, Rhepoxynius abronius. to Sediments Collected
In Conjunction with the Ketchican Pulp
9/27 - 10/7/88.
1
10-Day
Sampling Replicate Amphipod
Station Number Emergence
West Beach
Control
88390117 **
f, X
k/ ' )
88390118 ***
(\0-d}
V.
88390119 ***
(to-f]
88390120 **
C5o-\}
^
88390121 **
f&o-n
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
1
2
3
4
5
** 10-day amphipod
0
0
0
0
0
34
58
33
53
22
55
57
61
50
67
54
27
69
46
61
35
50
34
31
51
33
45
39
26
28
emergence and
x =
Sx ••
x =
Sx ••
X =
Sx
X =
Sx
X =
Sx
X -
Sx
and Paper Co. Project and Tested
Amphipod Responses
1
10-Day Amphipod Survival
1
No.
0.0
= 0.0
39.8
= 15.1
58.0
= 6.4
51.2
= 15.9
40.2
= 9.5
= 34.2
= 7.9
survival determi
| Survivors Not
of Survivors Mean % Reburying
20
20
20 x - 20.0 100.0
20 Sx = 0.0
20
11
11
11 x = 10.6 53.0
14 Sx = 2.9
6
9
10
10 x = 9.6 48.0
7 Sx = 1.8
12
11
7
9 x = 9.0 45.0
8 Sx = 1.6
10
13
14
10 x - 12.8 64.0
12 Sx - 1.9
15
1
3
0 x = 1.4 7.0
2 Sx = 1.1
1
ned to be significantly
0
0
0
0
0
1
4
1
3
1
2
3
3
2
5
3
2
5
1
1
1
0
1
2
3
0
3
-
0
0
different
_ _ \
x =
Sx =
X =
Sx =
X =
Sx =
X =
Sx =
X =
Sx
X =
Sx
0.0
= 0
2.0
= 1.4
3.0
= 1.2
2.4
= 1.7
1.4
= 1.1
0.75
= I-.5
from control at alpha = 0.05 (1-sided) by Dunnett's test (EPA Version 1.1).
*** 10-day amphipod emergence, survival, and reburial determined to be significantly
different from control at alpha = 0.05 (1-sided) by Dunnett's test (EPA Version 1.1)
D-15
-------
Table 2. Observations of Amphipod Emergence During the Ketchican Pulp and Paper
Amphipod-Sediment Toxicity Tests 9/27 - 10/7/88.
No. of Amphipods Out of Sediment
(Floating, Swimming, or On Sediment Surface)
Replicate
Treatment No.
1
2
West Beach 3
Control 4
5
1
2
88390117 * 3
4
5
1
2
88390118 * 3
4
5
1
2
88390119 * 3
4
5
1
2
88390120 * 3
4
5
1
2
88390121 * 3
4
5
123
000
000
000
000
000
454
10 8 11
940
12 8 7
340
13 8 6
11 6 1
10 10 8
12 8 1
11 11 9
798
2 10 1
7 7 10
836
969
3 7 5
984
11 6 5
12 7 4
5 7 7
963
10 6 7
565
453
633
4
0
0
0
0
0
1
7
4
1
2
4
3
3
6
6
9
2
12
5
11
4
7
5
3
5
0
3
4
4
1
Days
5 6
0
0
0
0
0
2
3
3
5
0
4
9
6
2
7
2
4
3
5
6
2
4
0
1
4
2
2
5
2
3
0
0
0
0
0
1
5
6
4
3
4
6
5
6
5
3
1
3
3
5
3
4
0
0
5
2
5
2
3
5
7
0
0
0
0
0
1
1
2
4
1
2
4
5
3
5
5
1
7
3
5
5
1
0
0
3
2
3
4
1
2
8
0
0
0
0
0
3
4
2
4
4
4
5
6
4
4
3
2
7
5
4
1
3
4
2
4
2
3
2
1
2
9
0
0
0
0
0
5
3
0
3
2
7
8
3
2
3
3
3
8
4
3
1
3
1
0
3
3
2
3
1
1
10
0
0
0
0
0
7
6
3
5
3
3
4
5
6
6
5
1
5
4
2
4
7
2
2
8
4
4
3
2
2
| 10-Day
Total Treatment
Emergence Summary
0
0
0 x = 0.0
0 Sx = 0.0
0
33
58
33 x = 39.8
53 Sx = 15.1
22
55
57
61 x = 58.0
50 Sx = 6.4
67
54
27
69 x = 51.2
46 Sx = 15.9
60
35
50
34 x = 40.2
31 Sx = 9.5
51
33
45
39 x = 34.2
26 Sx = 7.9
28
* 10-day amphipod emergence determined to be significantly greater than control at
alpha = 0.05 (1-sided) by Dunnett's test (EPA Version 1.1).
D-16
-------
Table 3. Selected Water Quality Characteristics Measured at the Beginning (Day 0)
and End (Day 10) of the Amphipod-Sediment Tests Conducted 9/27 - 10/7/88.
Day 0
DAY 10
1
Sampling DO Temperature Salinity
Station pH (mg/L) (°C) (°/°°)
West Beach
Control 7.92 7.2 15.4 28
88390117 8.11 7.4 15.3 28
88390118 8.04 7.7 15.4 28
88390119 7.82 7.0 15.4 28
88390120 7.98 7.4 15.1 28
88390121 8.19 7.0 15.3 28
1 1 1
DO Temperature Salinity
pH (mg/L) (°C) (°/°°)
7.97 7.7 15.2 28
8.07 7.4 15.1 28
8.10 7.7 15.1 28
7.91 7.7 15.3 28
8.13 8.0 15.1 28
8.21 8.1 15.1 28
D-17
-------
Table 4. Characteristics of Ward Cove Sediments Collected In Conjunction With the Ketchican
Pulp and Paper Company Project and Tested 9/27 - 10/7/88.
Sampling
Station
West Beach
Control
Interstitial Water
Salinity (o/oo)
28
PH
7.21
Wet Weight (g)
Per 2 cm Sediment
Layer Tested
282
General
Observations
Fine clean sand.
88390117
30
6.99
169
88390118
29
6.98
170
Fine black sediment with
small wood fibers, slight
H?S odor, water slightly
cloudy, slight oil sheen
on surface.
Fine black sediment with
small wood pieces, strong
H2S odor, water cloudy,
slight oil sheen on surface.
88390119
26
6.92
169
88390120
28
7.05
169
88390121
31
6.58
170
Fine black sediment with
many small/large wood
fibers and pieces, slight
H2S odor, water clear,
slight oil sheen on surface.
Fine black sediment with
many large wood chips and
pieces, strong ^S odor,
water cloudy, slight oil
sheen on surface.
Fine black sediment with
pulp-like wood fibers,
strong H2S odor, water
clear.
D-18
-------
WESTEC SERVICES
BIOASSAY LABORATORY
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88126 Sample ID: Ward Cove - Main
Facility Name: Ward Cove, Alaska
Facility Location: c/oEPAX _.. ,.--
12006thAvenue '., ;:; .' •-
Seattle, WA 98101 fa."'
Bioassay Specifications
A "• '!• ;•> p
Effluent Type: Final, pulp mill effluent
Test Type/Duration: Chronic/Sperm Fertilization 1.5 hours
Test Organism; common name: purple sea urchin
scientific name: Strongylocentrotus purpuratus
Summary of Final Results
Test Starting Date: 8/23/88 Completion Date: 8/23/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: <4.8% LOEC: 4.8%
Calculation Method: LCso/ECso: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: N/A
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control fertilization was between 60 -
90%
Test Organisms Data
Organism Source: Collected Mission Bay, San Diego, CA
Acclimation Period: >14 days Acclimation Mortality: 0
Organism Age at beginning of Test: N/A
D-19
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
4
Sperm to egg ratio of 200:1
10ml
EPA X personnel
grab
8/22/88
24 hours
Federal Express
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45(im
Test Certification
Laboratory Manager:
Date: I
Analyst:
Date:
any Snyder
AlanMonji
D-20
-------
cone.
WESTEC Bioassay Laboratory
Echinoderm Sperm Fertilization Bioassay Summary
WBL Test #
88126
Sample ID. # Ward Cove Main
rep. # eggs fertilized # eggs unfertilized % unfertilized avg. unfertilized
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
tf
a
b
c
d
a
b
c
d
87
86
79
90
81
88
90
85
62
72
48
72
50
57
60
37
0
2
0
0
0
0
0
0
1
0
0
0
13
1 5
21
1 0
1 9
1 2
1 0
1 5
38
28
52
28
50
43
40
62
100
98
100
1 00
1 00
100
1 00
100
99
1 00
100
1 00
13.0%
15.0%
21.0%
10.0%
19.0%
12.0%
10.0%
15.0%
38.0%
28.0%
52.0%
28.0%
50.0%
43.0%
40.0%
62.0%
100.0%
98.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
99.0%
100.0%
100.0%
100.0%
14.8%
14.0%
36.5%
48.8%
99.5%
100.0%
99.8%
D-21
-------
REVISION 1.00 5/1/87
THE DUNNETT COMPARISON OF EACH CONCENTRATION
1EAN VS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS /•"• / ^ Tx -/
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES OChwO(&^'rl ' *
KAY BE USED.
A ONE SIDED 'T' AT 95V. IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
NUMBER TRANSFORMATION
0 NONE
1 SQUARE ROOT(X)
3 LOGNKX)
5 (ARCSIN(SQ. RT(X)/(N+1)+
ARCSIN(SQ. RT"0.5>
NOTE :
THE LIMITS OF SIN ARE +/- 1 .
7 ALGAL ANALYSIS - NO TRANSFORMATION
• E S U L T S OF SEQUENTIAL COMPARISONS
USING THE D U N N E T T ' S TEST
CALCULATED DUNNETT T VALUES
lONCENTRATION CALC DUNNETT T
2 .1989224
3 -5.108259
4 -7.623344
5 -21.94471
4 -22.40015
7 -22.15157
TABULATED DUNNETT T = 2.45
MTE: H TABULATED DUNNETT T is greater than the
CALCULATED DUNNETT T, THERE IS NO SIGNIFICANT
DIFFERENCE between that CONCENTRATION and the
CONTROL. H the TABULATED DUNNETT T is less
than the CALCULATED DUNNETT T, then THERE IS
SIGNIFICANT DIFFERENCE between that CONCEN-
TRATION and the CONTROL.
For this set of data, the MINIMUM SIGNIFICANT DIFFERENCE is 0.075.
This represents a 51 . 1.X reduction o-f the mean response from the CONTROL.
There is NO S I GN I F I CANT DIFFERENCE between CONCENTRATION 2 and -CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 3 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIHCAiv! DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE D_22
-------
0.19
0.38
0.50
1.00
1.00
0.99
U.12
0.28
0.43
0.98
1.00
1.00
U.1U
0.53
0.40
1.00
1.00
1 .00
U.I 3
0.28
0.63
1 .00
1.00
1 .00
RANSFORMED DATA TABLE
NOTEiTRANSFQRMATloN NUMBER~6~WAS
USED FOR THIS SET OF DATA.
0.37
0.45
0.66
0.79
1.52
1.52
0.40
0.35
0.56
0.72
1.43
1.52
0.48
0.32
0.82
0.68
1 .52
1 .52
0.32
0.40
0.56
0.92
1 .52
1 .52
1.47 1.52 1.52 1.52
A N 0 V A TABLE
surce DF Sum o-f Sq . Mean Sq . Calc F Tab F<0.05)
'iONn e, c.'^o 1.129 222.017 2.570
i~L"'- 2! O.IC^ 0.005
D-23
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88127 Sample ID: Ward Cove Primary
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101 f:>«'/ '.;_-..*
* .- i '*•"
Bioassay Specifications ;- ;
n ov o;<
Effluent Type: Final, pulp mill effluent . (,,....,...,
Test Type/Duration: Chronic/Sperm Fertilization 1.5 hours ;''" V
.;;.,- .-7; - :;
Test Organism; common name: purple sea urchin
scientific name: Strongylocentrotus purpuratus
Summary of Final Results
Test Starting Date: 8/23/88 Completion Date: 8/23/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: <4.8% LOEC: 4.8%
Calculation Method: LCsQ/ECso: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: N/A
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control fertilization was between 60 -
90%
Test Organisms Data
Organism Source: Collected Mission Bay, San Diego, CA
Acclimation Period: >14 days Acclimation Mortality: 0
Organism Age at beginning of Test: N/A
D-24
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
4
Sperm to egg ratio of 200:1
10ml
EPA X personnel
grab
8/22/88
24 hours
Federal Express
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45|im
Test Certification
Laboratory Manager:
Date: \\ \\
Analyst:
Date: ///i/^V
iv/W" t^OC'1- n o J
•'v"/(^ Bany Snyder
Alan Monji
D-25
-------
cone.
WESTEC Bioassay Laboratory
Echinoderm Sperm Fertilization Bioassay Summary
WBLTesttf 88127 Sample ID. # Ward Cove Primary
rep. # eggs fertilized # eggs unfertilized % unfertilized avg. unfertilized
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
dr
a
b
c
d
a
b
c
d
87
86
79
90
81
88
90
85
78
74
67
56
42
28
31
29
2
2
4
1
0
0
0
0
0
1
0
0
1 3
1 5
21
1 0
1 9
1 2
1 0
1 5
22
26
33
44
58
72
69
71
98
98
96
99
1 00
100
100
100
100
99
1 00
1 00
13.0%
15.0%
21.0%
10.0%
19.0%
12.0%
10.0%
15.0%
22.0%
26.0%
33.0%
44.0%
58.0%
72.0%
69.0%
71.0%
98.0%
98.0%
96.0%
99.0%
100.0%
100.0%
100.0%
100.0%
100.0%
99.0%
100.0%
100.0%
14.8%
14.0%
31 .2%
67.5%
97.8%
100.0%
99.8%
D-26
-------
;:CN 1.00 5/1/87
ANOVA-UITH THE DUNNETT COMPARISON OF EACH CONCENTRATION
MEAN MS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS ,
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES CAftAjb (7&\
MAY BE USED. ^-^
A ONE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
NUMBER TRANSFORMATION
0 NONE
1 SQUARE ROOT(X)
3 LOGIO(X)
5
NOTE :
THE LIMITS OF SIN ARE +/- 1.
7 ALGAL ANALYSIS - NO TRANSFORMATION
RESULTS OF SEQUENTIAL COMPARISONS
USING THE DUNNETT S TEST
CALCULATED DUNNETT T VALUES
CONCENTRATION CALC DUNNETT T
2 .2373651
3 -4.70918
4 -13,53716
5 -24.45033
6 -26.72909
1 -26.43247
TABULATED DUNNETT T = 2.45
NOTE: I-f TABULATED DUNNETT T is greater than the
CALCULATED DUNNETT T, THERE IS NO SIGNIFICANT
DIFFERENCE between that CONCENTRATION and the
CONTROL. If the TABULATED DUNNETT T is less
than the CALCULATED DUNNETT T, then THERE IS
SIGNIFICANT DIFFERENCE between that CONCEN-
TRATION and the CONTROL.
For this set o-f data, the MINIMUM SIGNIFICANT DIFFERENCE is 0.065.
This represents a 44. OX reduction o-f the mean response -from the CONTROL.
There is NO SIGNIFICANT DIFFERENCE between CONCENTRATION 2 and_CONT
=>There IS A SIGNIFI CANT DIFFERENCE between CONCENTRATION 3 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFI CANT DIFFERENCE between CONCENTRATION 6 and CONT
OThere IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE D-27
-------
u.i*
0.22
0.58
0.98
1.00
u.i^:
0.26
0.72
0.98
1.00
U . 1U
0.33
0.69
0.96
1.00
U. 13
0.44
0.71
0.99
1.00
i.OO 0.99 1.00 1.00
RANSFORMED DATA TABLE
NOTETTRANSFORMATlON NUMBER~6~WAS
USED FOR THIS SET OF DATA.
0.37 0.40 0.48 0.32
0.45 0.35 0.32 0.40
0.49 0.54 0.61 0.73
0.87 1.01 0.98 1.00
1.43 1.43 1.37 1.47
1.52 1.52 1.52 1.52
1.52 1.47 1.52 1.52
A NOVA TABLE
Source DF Sum o-f Sq . Mean Sq . Calc F Tab F(0.05)
ttONG 6 6.504 1.084 303.408 2.570
•IITHIN 21 0.075 0.004
TOTAL 27 6.579
D-28
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBL Test No.: 88128 Sample ID: Ward Cove - Secondary
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X ...--;" < ..
1200 6th Avenue , . , :..
Seattle, WA 98101 O/j.
Bioassav Specifications '" '' 'J
Effluent Type: Final, pulp mill effluent '""' '*'•''
Test Type/Duration: Chronic/Sperm Fertilization 1.5 hours ''
Test Organism; common name: purple sea urchin
scientific name: Strongylocentrotus purpuratus
Summary of Final Results
Test Starting Date: 8/23/88 Completion Date: 8/23/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: <4.8% LOEC: 4.8%
Calculation Method: LCso/ECsQ: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: N/A
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control fertilization was between 60 -
90%
Test Organisms Data
Organism Source: Collected Mission Bay, San Diego, CA
Acclimation Period: > 14 days Acclimation Mortality: 0
Organism Age at beginning of Test: N/A
D-29
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
4
Sperm to egg ratio of 200:1
10ml
EPA X personnel
grab
8/22/88
24 hours
Federal Express
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45|im
Test Certification
Laboratory Manager:
Date: ii 1*188
Analyst:
Date:
Barry Snyder
Alan Monji
D-30
-------
cone.
WESTEC Bioassay Laboratory
Echinoderm Sperm Fertilization Bioassay Summary
WBL Test # 88128
Sample ID. # Ward Cove Secc
rep. # eggs fertilized # eggs unfertilized % unfertilized avg. unfertilized
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
87
86
79
90
81
88
90
85
45
50
49
4 1
4
0
0
1 2
0
0
0
0
0
0
0
0
0
0
0
0
1 3
1 5
21
1 0
1 9
1 2
1 0
1 5
22
26
33
44
96
1 00
100
88
100
1 00
100
1 00
100
100
1 00
100
100
1 00
100
1 00
13.0%
15.0%
21.0%
10.0%
19.0%
12.0%
10.0%
15.0%
22.0%
26.0%
33.0%
44.0%
96.0%
100.0%
100.0%
88.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
14.8%
14.0%
31.2%
96.0%
100.0%
100.0%
100.0%
D-31
-------
#
REVISION 1.00 5/1/87
ANQVA-WITH THE DUNNETT COMPARISON OF EACH CONCENTRATION
MEAN VS THE CONTROL MEAN. UP TO SEMEN CONCENTRATIONS
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES
HAY BE USED.
A ONE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE L^^ ON THE DATA ARE:
NUMBER TRANSFORMAT I ON
0 NONE
i SQUARE ROOT(X)
3 LOGIO(X)
5 There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 3 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and CQNT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE D-32
-------
0.1?
0.50
0.96
1.00
1.00
1.00
0.12
0.50
1.00
1 .00
1.00
1.00
0.10
0.51
1.00
1 .00
1.00
1 .00
0.15
0.5?
0.88
1 .00
1 .00
1 .00
RANSFQRMED DATA TABLE
NUMBER6WAS
USED FOR THIS SET OF DATA.
0.37 0.40 0.48 0.32
0
0
1
1
1
1
.45
.79
.37
.52
.52
.52
0
0
1
1
1
1
.35
.79
.52
.52
.52
.52
0
0
1
1
1
1
.32
.80
.52
.52
.52
.52
0
0
1
1
1
1
.40
.88
.22
.52
.52
.52
AN QUA TABLE
ource DF Sum o-f Sq. Mean Sq . Calc F Tab FO3.05)
MONG 6 6.902 1.150 265.055 2.570
HTHIN 21 0.091 0.004
'DIAL 27 6.993
D-33
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88185 Sample ID: Ward Cove - 88390101
(Main 001)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue .-..-,,..-.>-
Seattle, WA 98101 ^.,.:"
BioassavSpecifications :; \ ,,-.,,,
'••'"'"" iiiiV H-^Mo'
Effluent Type: Final, pulp mill effluent
Test Type/Duration: Chronic/Sperm Fertilization - 1.5 hours , ••• r , '- ^
Test Organism; common name: purple sea urchin
scientific name: Strongylocentrotus purpuratus
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/22/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: 4.8% LOEC: 9.6%
Calculation Method: LC50/EC50: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: N/A
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control fertilization was between 60 -
90%
Test Organisms Data
Organism Source: Collected - Mission Bay, San Diego, CA
Acclimation Period: > 14 days Acclimation Mortality: 0
Organism Age at beginning of Test: N/A
D-34
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
4
Sperm to egg ratio of 200:1
10ml
EPA X personnel
composite
9/20/88
48 hours
Alaska Airlines
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45|im
Test Certification
Laboratory Manager.
Date:
Analyst:
Date: n / *• fa
'Barry Snyder
Alan Monji
' AA^^y
D-35
-------
WESTEC Bioassay Laboratory
Echinoderm Sperm Fertilization Bioassay Summary
WBLTest* 88185 Sample ID. # 88390101
cone. rep. # eggs fertilized # eggs unfertilized % unfertilized avg. unfertilized
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
cr
a
b
c
d
a
b
c
d
89
76
69
86
93
87
84
86
84
80
76
78
52
69
57
52
1 6
2 1
3 1
1 4
0
0
0
0
0
0
0
0
1 1
24
31
1 4
7
1 3
1 6
1 4
1 6
20
24
22
48
31
43
48
84
79
69
86
100
1 00
100
100
1 00
1 00
100
1 00
1 1 .0%
24.0%
31.0%
14.0%
7.0%
13.0%
16.0%
14.0%
16.0%
20.0%
24.0%
22.0%
48.0%
31.0%
43.0%
48.0%
84.0%
79.0%
69.0%
86.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
20.0%
12.5%
20.5%
42.5%
79.5%
100.0%
100.0%
D-36
-------
REVISION 1.00 5/1/87
ANtWA-WITH THE DUNNETT COMPARISON OF EACH CONCENTRATION ftPfiO ,n t
MEAN VS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS U(/J I U I
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES
MAY BE USED.
A ONE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
NUMBER TRANSFORMATION
0 NONE
1 SQUARE ROOT(X)
3 LOGIO(X)
5 ':ARCSIN/ +
ARCSIN(SQ. RTThere IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and CONT
=''There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIFI CANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE D-37
-------
o.u
0.48
0.84
1.00
1.00
0.20
0.31
0.79
1.00
1.00
0.24
0.43
0.69
1.00
1.00
0.22
0.48
0.86
1 .00
1 .00
RANSFORMED DATA TABLE
NOTETTRANSFORMATlON NUMBER~6~WAS
USED FOR THIS SET OF DATA.
0.34 0.51 0.59 0.38
0.27
0.41
0.77
1.16
1.52
1.52
0.37
0.46
0.59
1.09
1.52
1.52
0.41
0.51
0.72
0.98
1.52
1.52
0.38
0.49
0.77
1 .19
1 .52
1.52
A N 0 V A TABLE
Source DF Sum o-f Sq. Mean Sq. Calc F Tab F<0.05)
WONG 6 6.090 1.015 205.893 2.570
4ITH1N 21 0.104 0.005
TOTAL 27 6.194
D-38
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88186 Sample ID: Ward Cove - 88390104
(Primary 002A)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101 . , \ :f : :
'"'••"> v •.'.'.>'.-.'••
Bioassay Specifications (!
Effluent Type: Final, pulp mill effluent ' ! ! w :-' ' H? '
Test Type/Duration: Chronic/Sperm Fertilization - 1.5 hours
Test Organism; common name: purple sea urchin : '
scientific name: Strongylocentrotus purpuratus
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/22/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: 4.8% LOEC: 9.6%
Calculation Method: LCso/ECsQ: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: N/A
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control fertilization was between 60 -
90%
Test Organisms Data
Organism Source: Collected Mission Bay, San Diego, CA
Acclimation Period: >l4days Acclimation Mortality: 0
Organism Age at beginning of Test: N/A
D-39
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
4
Sperm to egg ratio of 200:1
10ml
EPA X personnel
composite
9/20/88
48 hours
Alaska Airlines
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45}im
Test Certification
i -j -
Laboratory Manager: £) L-iAAw
• , ^'
Date: /,/; /^
Analyst:
Date:
I
^-
Barry Snyder
Alan Monji
D-40
-------
WESTEC Bioassay Laboratory
Echinoderm Sperm Fertilization Bioassay Summary
WBLTesttf 88186 Sample ID. # 88390104
cone. rep. # eggs fertilized # eggs unfertilized % unfertilized avg. unfertilized
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
89
76
69
86
93
87
84
86
57
73
86
81
52
51
42
63
3
4
9
1 6
0
0
0
0
0
0
0
0
1 1
24
31
1 4
7
13
1 6
14
43
27
1 4
1 9
48
49
58
37
97
96
9 1
84
100
100
100
1 00
1 00
100
100
1 00
1 1 .0%
24.0%
31.0%
14.0%
7.0%
13.0%
16.0%
14.0%
43.0%
27.0%
14.0%
19.0%
48.0%
49.0%
58.0%
37.0%
97.0%
96.0%
91.0%
84.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
20.0%
12.5%
25.8%
48.0%
92.0%
100.0%
100.0%
D-41
-------
REVISION 1.00 5/1/87
NOYA-UITH THE DUNNETT COMPARISON OF EACH CONCENTRATION Q Q Q fll ftd-
1EAN VS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS U O 5 / ^
'INCLUDING THE CONTROL) AND UP TO TEN REPLICATES x- / /
IAY BE USED . zr£ k^y^O C&*
\ WE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES. X Q, (.0*4!$-
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
1UM6ER TRANSFORMATION
0 NONE
1 SQUARE ROOT(X)
3 LOGIO(X)
5 (ARCSIN(SQ. RT':X)/(N+i) +
ARCSIN(SQ. RTCXM )/(N-H)))/2
6 ARCSIN(O<) -0.5)
NOTE :
THE LIMITS OF SIN ARE +/- 1.
7 ALGAL ANALYSIS - NO TRANSFORMATION
ESULTS OF SEQUENTIAL COMPARISONS
USING THE DUNNETT'S TEST
CALCULATED DUNNETT T VALUES
lONCENTRATION CALC DUNNETT T
2 1.53299
3 -i.06286
4 -4.830256
5 -13.156
4 -16.63912
7 -16.63912
TABULATED DUNNETT T = 2.45
WTE: If TABULATED DUNNETT T is greater than the
CALCULATED DUNNETT T. THERE IS NO SIGNIFICANT
DIFFERENCE between that CONCENTRATION and the
CONTROL. I-f the TABULATED DUNNETT T is less
than the CALCULATED DUNNETT T, then THERE IS
SIGNIFICANT DIFFERENCE bet^esr. that CONCEN-
TRATION and the CONTROL.
For this set o-f data, the MINIMUM SIGNIFICANT DIFFERENCE is 0.107.
This represents a 53.57. reduction o-f the mean response -from the CONTROL.
There is NO SI Gf 41FI CANT DIFFERENCE between CONCENTRATION 2 and-COT-IT
There is NO SIGNIFICANT DIFFERENCE between CONCENTRATION 3 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE D-42
-------
U .4J
0.48
0.97
1.00
1.00
U .£.(
0.49
0.?6
1 .00
1 .00
u . 11
0.5S
0.91
1 .00
1 .00
u . i y
0.37
0.84
1 .00
1 .00
= FORMED DATA
ABLE
NOTE:TRANSFORMATION NUMBER 6 WAS
USED FOR THIS SET OF DATA.
0.77
1.40
1.52
1.52
0.51
0.37
0 . 55
0.78
1 .37
1 .52
1 .52
0.59
0.41
0.38
0.87
1 .27
1 .52
1 .52
0.38
0.38
0.45
0.65
1.16
1 .52
1 .52
A N 0
OF
WONG 6
.-ilTHIN 21
TOTAL 27
Sum o-f Sq.
6.307
0.172
6.479
TABLE
Mean Sq .
1.051
0.008
Calc F Tab F(0.05)
128.344 2.570
D-43
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88187 Sample ID: Ward Cove - 88390107
(Secondary 002B)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101
Bioassay Specifications ._..
.-- - " u
Effluent Type: Final, pulp mill effluent •• "] -,' y -
Test Type/Duration: Chronic/Sperm Fertilization - 1.5 hours ',;(.""
vw* uoV
Test Organism; common name: purple sea urchin
scientific name: Strongylocentrotus purpuratus ..... -,~ ? •-_.;;• ',-.;> f
1 1 • - -
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/22/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: <4.8% LOEC: 4.8%
Calculation Method: LCso/ECsQ: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: N/A
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control fertilization was between 60 -
90%
Test Organisms Data
Organism Source: Collected Mission Bay, San Diego, CA
Acclimation Period: > 14 days Acclimation Mortality: 0
Organism Age at beginning of Test: N/A
D-44
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Sperm to egg ratio of 200:1
10ml
EPA X personnel
composite
9/20/88
48 hours
Alaska Airlines
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45jim
Test Certification
Laboratory Manager:
Date: ////
Analyst:
Date: /
Barry Snyder
Alan Monji
^ T
D-45
-------
WESTEC Bioassay Laboratory
Echinoderm Sperm Fertilization Bioassay Summary
WBL Test # 88187 Sample ID. # 88390107
cone. rep. # eggs fertilized # eggs unfertilized % unfertilized avg. unfertilized
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
a
b
c
d
89
76
69
86
93
87
84
86
61
60
42
39
28
20
1 8
36
0
0
0
1
0
0
0
0
0
0
0
0
1 1
24
31
1 4
7
1 3
1 6
1 4
39
40
58
61
72
80
82
64
100
1 00
1 00
99
1 00
100
100
100
1 00
100
100
1 00
1 1 .0%
24.0%
31.0%
14.0%
7.0%
13.0%
16.0%
14.0%
39.0%
40.0%
58.0%
61.0%
72.0%
80.0%
82.0%
64.0%
100.0%
100.0%
100.0%
99.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
20.0%
12.5%
49.5%
74.5%
99.8%
100.0%
100.0%
D-46
-------
0&/0
THE CONTROL) AND UP TO TEN REPLICATES , .
,Y BE USED. ^™
ONE SIDED 'T-' AT 95X IS ASSL1MED IN ALL CASES. $$3*?
M/-
'HE
TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
(UMBER TRANSFORMATION ,
0 NONE / ${.CO*IS-
j SQUARE ROOKX) L
- LOG1 OCX)
5 (ARCSIN(SQ. RTThere IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 3 and CONT
:>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and "CONT
:>There IS A SIGNIFI CAT4T DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE
D-47
0.11
0.07
0.39
0.24
0.13
0.40
0.31
0.16
0.58
0.14
0.14
0.61
-------
i.oo i.oo i.oo i.oo
1.00 1.00 1.00 1.00
RANSFORMED DATA TABLE
NOTETTRANSFORMATlON NUMBER~6~WAS
USED FOR THIS SET OF DATA.
0.34
0.27
0.67
1.01
1.52
1.52
1.52
0.51
0.37
0.68
1 .11
1.52
! .52
1.52
0.59
0.41
0.87
1 .13
1.52
1 .52
1 .52
0.38
0.38
0.90
0.93
1 .47
r.52
1 .52
A N 0 I.' A TABLE
:urce DF Sum o-f Sq . Mean Sq. Calc F Tab FvO.OS)
10N6 6 6.21c. 1.036 179.004 2.570
ITHIN 2: 0.122 0.006
3TAL 27 6.338
D-48
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88182 Sample ID: Ward Cove - 88390105
(Primary 002A)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101
BioassaySpecifications •'•"; ; -.; ;,::, ;
Effluent Type: Final, pulp mill effluent •-•••- y,^-- O'Mr
Test Type/Duration: Static acute - 96hours "*'-''•' '-''' '3
Test Organism; common name: rainbow trout " ''''',
scientific name: Salmo gairdneri
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/26/88
LC50/EC50: 35.3% effluent 95% confidence intervals: 27.8% - 46.9%
NOEC: N/A LOEC: N/A
Calculation Method: LC^Q/EC^Q: Moving Average
NOEC/LOEC: N/A
Quality Control Summary
Control Mortality: 5 %
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control mortality less than 10%
Test Organisms Data
Organism Source: Thomas Fish Farm, Novato, CA
Acclimation Period: 7 days Acclimation Mortality: <1.0%
Organism Age at beginning of Test: approx. 30 days
D-49
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Mortality Data (percent mortality)
Test Concentration
control
6.25%
12.5%
- 25.0%
50.0%
100%
rep.
a
b
a
b
a
b
a
b
a
b
a
b
%Moi
10
0
10
0
0
10
0
10
70
100
100
100
5
2
10
2 liters
EPA X personnel
grab
9/20/88
48 hours
Alaska Airlines
WBL laboratory grade freshwater
charcoal filtered and aerated
Avg. Mortality (%}
5
5
5
5
85
100
Test Certification
Laboratory Manager:
Date: ///2
Analyst:
Date: /' A
Snyder
MonJi
D-50
-------
DATA SHEET FOR EFFLUENT TOXICITY TEST.
Indullly •To.iclnl ^"Pft X /. Jnrr.l f'^.lf H ft* .^ ^ p 7/0
Add....
Tlm"
Elllucnt Stiiil Number
NPDES fermil Nurnbci
Beginning:
Ending: n... !l/lf- — T I m e
Of9»ni»m
Ten Tempeiiture fling « li ° - 3.°?^
^onc.
Ot
%
A/*-"
(.•i'S'f.
li.
\Q
Io
l«
I"
lu
K?
10
24
(O
/O
/O
/o
^
/O
&
O
48
1
1°
*\
to
10
°l
\o
*\
4
7-
—
-r
72
1
/"
1
/o
1°
1
/o
1
4
I
—
—
90
1
1 ^
1
io
10
1
Ic.
«\
^
O
-
—
Oiisolved Oiygen
(mg/l)
0
nJ.>
"M
^f
r^u
•I.*
'.-TT*
%
n
24
t2i_C
10,0
•I'p;
1C. 6
1-tf
IPi'i
•>.6
'i.fr
O.'i
1-H
i,^r
q,(r
48
IP' Ir*
(0.0
lp.4-
•||.o
ll-o
l».e
/i.o
(0,6
|C.^
/o.e
—
—
72
l°'1
|0l
|o(.
IO)
(02
(O-t
l»l
li.'O
PJ
/^
—
—
90
f.?
?.
it
<,?*>
\.1o
(fOO
ls)0
io 00
(jV>
',10
koc
&
tfO
bCO
to
6rt>
—
—
96
i^
MTJ
^'fl
vw
^05
H?=-
tfr-V
•f*
(fPT
'7^
—
[YifyX-'ruLure ( (!)
0
"j-y^o
lift
/?,[
//•<»
M,n
/?,^
24
(t-^
k?,6.
10-1
h,f
fa'^r
loM
*7>'^
fr>^
^•f
W
^•T
^ri^
48
/J-Z-
//.-?
/^.^
fl.^
/».
//>
ti.lf
!!•*>
ll. t.
II. u
—
72
/#y
/^c
p-f
(l-l
121
HI
U4
u-i
M-o
ft.'
—
—
96
D.fi
io n
o.r,
ti
!%•
to-'
1*}
fa-l
/p,
f?if*
—
a
i
r-,,i.s
^ t/ (T-'c^ / V . i
m
-------
C. NUMBER NUMBER PERCENT BINOMIAL
EXPOSED DEAD DEAD PROB.(X)
30 20 20 100 9.536743E-05
0 20 17 85 .1288414
5 20 1 5 2.002716E-03
2.5 20 1 5 2.002716E-03
.25 20 1 5 2.002716E-03
E BINOMIAL TEST SHOWS THAT 25 AND 50 CAN BE
ED AS STATISCALLY SOUND CONSERVATIVE 95 PERCENT
NFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL
SOCIATED WITH THESE LIMITS IS 9?.86916 PERCENT.
APPROXIMATE LC50 FOR THIS SET OF DATA IS 37.53657
»»»RESULTS CALCULATED USING THE MOVING AVERAGE METHOD
AN G LC50 95 PERCENT CONFIDENCE LIMITS
6.572942E-02 35.2711 27.33709 46.908
»>»RESULTS CALCULATED USING THE PROBIT METHOD
ERAT10NS G H GOODNtSC CF FIT PROBABILITY
3.18085 10.81941 0
PROBABILITY OF 0 MEANS THAT IT IS LESS THAN 0.00!
NCE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED
iING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED.
.OPE = 3.946269
i PERCENT CONFIDENCE LIMITS =-3.091877 AND 10.98442
i50 = 33.89604
i PERCENT CONFIDENCE LIMITS = 0 AND t INFINITY
:i = 8.720297
3 PERCENT CONFIDENCE LIMITS = 0 AND 27.82212
D-52
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
S an Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88183 SamplelD: Ward Cove - 88390102
(Main 001)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue ,- - ,: "' '• /; -: --.. -
Seattle, WA 98101 '•'' 'Y -'.'••• :':-'. .
Bioassav Specifications ' Jl'lv/' i")
Effluent Type: Final, pulp mill effluent .. '•' >'--.\*iK •: ''/•>.;
Test Type/Duration: Static acute - 96hours : .•, .'/ ^.;. ."••'.
Test Organism; common name: rainbow trout
scientific name: Salmo gairdneri
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/26/88
LC50/EC50: 3 1.9% effluent 95% confidence intervals: not available
NOEC: N/A LOEC: N/A
Calculation Method: LC^Q/EC^Q: Linear interpolation
NOEC/LOEC: N/A
Quality Control Summary
Control Mortality: 5 %
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control mortality less than 10%
Test Organisms Data
Organism Source: Thomas Fish Farm, Novato, CA
Acclimation Period: 7 days Acclimation Mortality: <1.0%
Organism Age at beginning of Test: approx. 30 days
D-53
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Mortality Data (percent mortality)
Test Concentration rep.
5
2
10
2 liters
EPA X personnel
grab
9/20/88
48 hours
Alaska Airlines
WBL laboratory grade freshwater
charcoal filtered and aerated
% Mortality
control
6.25%
12.5%
' 25.0%
50.0%
100%
a
b
a
b
a
b
a
b
a
b
a
b
10
0
0
0
0
0
30
0
100
100
100
100
Avg. Mortality (%)
5
0
0
15
100
100
Test Certification
Laboratory Manager:
Date:
Analyst:
Date: / •
Barry Snyder
Alan Monji
D-54
-------
DATA SHEET FOR EFFLUENT TOXICITY TEST.
Industry
Addrtss
Coniaei
To.irani -ffYlX C,J|1-£D (tOLIf "rC&y^O
Seafyls , tut/ tl.
flttb lf'<'&&*'S&si
ElMuent Serial Numb
NPOES Permit Numbi
O
1
Ui
U1
^onc.
or
%
/^•/
£-2*-
n .*r
45"
Ctt
/O£>
Test
Containei
Numbei
„{
6
ri
A
/3
ft
/2
&
4 f
B
A t
/?
., UJP>L & H&IVS
t
Number ol
Live Organisms
0
((>
to
(0
fA
(n
to
(O
to
IQ
/o
1C?
(0
24
(<)
/O
(0
10
1°
10
|0
If)
p
itf
10°
fi>*
48
f
1°
to
/o
/o
/o
3-
/o
—
—
—
—
72
q
/^
It?
l»
l«?
r<>
1
/o
—
—
—
—
36
1
11?
10
\t~,
(f\
If'
7*
/0_
—
—
—
1C. 3. (5;^: 2J
Beginning:
Ending:
Test Orgini
Test Tempe
Dissolved OiyQcn
(mg/l)
0
/o-c>
|o
11
1-i
Vt
7ft'
/7/r
7<^P
7.«?
P
S '
go
^
So
Bo
—
—
—
—
96
W
fir5*
7-^
TW
T? 5
],-|'i
Mr.
If/I
—
—
—
Toul Alkalinity
|mg/l as CaCO 3 I
0
|1
,1
tti1
24
)
48
72
90
O A II
•J/30 Tim« ^.'00/3.^-
n,,. fe/Aft rim. /^'rfC'X^
$m _
P^{; „ A--K.J ^^^ , /
raluie Ran
9. /J0^* i ^V
>
Total Hardness
0
47?
/
^
(
V
s'r?
"TV?
iot>
/
llfin
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-------
Tn)ui
ONC.
100
50
25
12.5
6.25
NUMBER
EXPOSED
20
20
20
20
20
NUMBER
DEAD
20
20
3
0
0
HE BINOMIAL TEST SHOWS THAT 25 AND 50
SED AS STATISCALLY SOUND CONSERVATIVE 95
PERCENT
DEAD
100
100
15
0
0
CAN BE
PERCENT
BINOMIAL
PROB.CX)
9.536743E-05
9.536743E-05
.1288414
9.536743E-05
ONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL
iSSOCIATED WITH THESE LIMITS IS 99.87106 PERCENT.
N APPROXIMATE LC50 FOR THIS SET OF DATA IS 31.89303
IHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT
•EAO IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NOR THE
'ROBIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS.
D-56
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88184 Sample ID: Ward Cove - 88390108
(Secondary 002B)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th A venue
Seattle, WA 98101 ---• 7""V<9;,.
Bioassay Specifications ;.'.'
' ' '••''. :'•,' 0 '
Effluent Type: Final, pulp mill effluent
Test Type/Duration: Static acute - 96hours ,: , r,:,:,.,: x ,; . ,. , -
^ . . fi-'i- •';' -''' :r'
Test Organism; common name: rainbow trout
scientific name: Salmo gairdneri
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/26/88
LC50/EC50: 77.5% effluent 95% confidence intervals: 56.0% 100%
NOEC: N/A LOEC: N/A
Calculation Method: LC50/EC5Q: Moving Average
NOEC/LOEC: N/A
Quality Control Summary
Control Mortality: 5 %
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control mortality less than 10%
Test Organisms Data
Organism Source: Thomas Fish Farm, Novato, CA
Acclimation Period: 7 days Acclimation Mortality: <1.0%
Organism Age at beginning of Test: approx. 30 days
D-57
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Mortality Data (percent mortality)
Test Concentration
5
2
10
2 liters
EPA X personnel
grab
9/20/88
48 hours
Alaska Airlines
WBL laboratory grade freshwater
charcoal filtered and aerated
% Mortality
control
6.25%
12.5%
' 25.0%
50.0%
100%
— • a -
a
b
a
b
a
b
a
b
a
b
a
b
10
0
0
10
10
0
0
0
20
30
90
40
Avg. Mortality (%)
5
5
5
0
25
65
Test Certification
Laboratory Manager:
Date: />
Analyst:
Date: /'A
Barry Snyder
Alan Monji
D-58
-------
DATA SHEET FOR EFFLUENT TOXICITY TEST.
Indulll. To.icanl
Contact
Elllueni Serial Number
NPDES Permit Number
eginning: fi«i« 7/3.3. Time,
ndlng: n.i. ci/3-L T'Tt
I til-
8
Ending
Ten Temperature Rings.
Ten
Container
Number
Number ol
Live Organisms
0 24
48
72 96
Oi isolved Oiygen
Img/l)
24
48
72
96
PH
24
72
96
Total Alkalinity
Img/l ai CaCO3 I
24
48
72
96
T
btal Hardness (Conductivity
24
48
72
96
24
48
72 96
( (!)
24
48
72 96
JL
lo
0.4
j£i
111
liA
Mo
la-
11;
in
10.
(0
to i>
(p.4-
7m
M-n
tt/
l.p
in.
10
<£L
0 0
lo
D
I
Ol
a -sr
1LL
$3-
10.4-
flop
SO?
JL
/O.i
1LL
Ii2.
1A
lot a
(0-30
We
Hi
lo
/0.4-
10 0
m&
ID.
10'!
/410
IflO
te*
2.
n_a
*
77
10-0
81
JisriZ^o
2200
//.(x
ll-
ifij
M
JiL
B.'b
7to-asp
[l-t
^i
j
-
-------
NUMBER NUMBER PERCENT BINOMIAL
EXPOSED DEAD DEAD PROB.(X)
100 20 13 65 13.1588 -?
50 20 5 25 2.069473
25 20 0 0 9.536743E-05
12.5 20 1 5 2.002716E-03
6.25 20 1 5 2.002716E-03
THE BINOMIAL TEST SHOWS THAT 50 AND + INFINITY CAN BE
USED AS STATISCALLY SOUND CONSERVATIVE 95 PERCENT
CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL
ASSOCIATED WITH THESE LIMITS IS 97.93053 PERCENT.
AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 77.45697
»»»»RESULTS CALCULATED USING THE MOVING AVERAGE METHOD
SPAN G LC50 95 PERCENT CONFIDENCE LIMITS
1 .6047553 77.45697 55.9507 141.7173
>»»»RESULTS CALCULATED USING THE PROBIT METHOD
ITERATIONS 6 H GOODNESS OF FIT PROBABILITY
6 1.633447 2.871438 3.488326E-02
SINCE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED
USING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED.
SLOPE = 2.091501
95 PERCENT CONFIDENCE LIMITS =-.5815709 AND 4.764572
LC50 = 88.99493
95 PERCENT CONFIDENCE LIMITS = 28.80873 AND + INFINITY
LCI = 6.868585
95 PERCENT CONFIDENCE LIMITS = 0 AND 24.11993
D-60
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBL Test No.:
Facility Name:
Facility Location:
88188
Ward Cove, Alaska
c/o EPA X
1200 6th Avenue
Seattle, WA 98101
Sample ID:
Bioassay Specifications
Effluent Type: Final, pulp mill effluent
Test Type/Duration: Chronic/Bivalve Larvae - 48 hours
Test Organism;
common name:
scientific name:
Summary of Final Results
9/22/88
N/A
<4.8%
Test Starting Date:
LC50/EC50:
NOEC:
Calculation Method:
NOEC/LOEC:
Quality Control Summary
Control Mortality: 24.3%
Temperature maintained:
Ward Cove - 88390101
(Main 001)
Pacific oyster
Crassostrea gigas
Completion Date:
95% confidence intervals:
LOEC:
N/A
Dunnett's Procedure
9/24/88
N/A
4.8%
yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: no - control mortality should be less than 20%
Test Organisms Data
Organism Source: Brood stock from Brezina and Associates, Dillon Beach, CA
Acclimation Period: >14days Acclimation Mortality: 0
Organism Age at beginning of Test: larvae less than 3 hours
D-61
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
3
25 larvae per ml
100ml
EPA X personnel
composite
9/20/88
48 hours
Alaska Airlines
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45(im
Test Certification
Laboratory Manager
Date:
Analyst:
Date:
Barry Snyder
Alan Monji
/
D-62
-------
WESTEC Bioassay Laboratory
Bivalve Larvae Bioassay Summary
WBLTesttf 88188 Sample ID. # 88390101 (Main 001)
cone. rep. # normal # abnormal total % abnormal avg. abnormal % survival
1.0% 75.7%
6.0% 77.5%
32.3% 56.9%
43.0% 23.4%
0.0% 0.0%
0.0% 0.0%
0.0% 0.0%
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
r
a
b
c
142
191
175
1 16
179
210
79
142
66
3
9
1 7
0
0
0
0
0
0
0
0
0
0
2
1
4
0
1 4
32
26
39
42
23
64
0
0
0
0
0
0
0
0
0
142
1 93
176
1 20
179
224
1 1 1
1 68
105
45
32
8 1
0
0
0
0
0
0
0
0
0
0.0%
2.0%
1 .0%
4.0%
0.0%
14.0%
32.0%
26.0%
39.0%
42.0%
23.0%
64.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
D-63
-------
REVISION 1.00 5/1/87
ANWA-WITH THE DUNNETT COMPARISON OF EACH CONCENTRATION (Jj&A'ct £0*
MEAN VS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES M
MAY BE USED. '
A ONE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
NUMBER TRANSFORMATION
Q NONE
1 SQUARE ROOT*0.5>
NOTE :
THE LIMITS OF SIN ARE +/- 1.
7 ALGAL ANALYSIS - NO TRANSFORMATION
RESULTS OF SEQUENTIAL COMPARISONS
USING THE DUNNETT' S TEST
CALCULATED DUNNETT T VALUES
CONCENTRATION CALC DUNNETT T
2 -1.276255
3 -5.71658
4 -6.914319
5 -16.14711
6 -16.14711
7 -16.14711
TABULATED DUNNETT T = 2.53
NOTE: H TABULATED DUNNETT T is greater than the
CALCULATED DUNNETT T, THERE IS NO SIGNIFI CANT
DIFFERENCE between that CONCENTRATION and the
CONTROL. H the TABULATED DUNNETT T is less
than the CALCULATED DUNNETT T, then THERE IS
SIGNIFICANT DIFFERENCE between that CONCEN-
TRATION and the CONTROL.
For this set o-f data, the MINIMUM SIGNIFICANT DIFFERENCE is -0.010.
This represents a-96.3X reduction o-f the mean response -from the CONTROL.
There is NO SI GN 1FI CAT-IT DIFFERENCE between CONCENTRATION 2 and CONT
~>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 3 and-CONT
=>There IS A SIGNIFI CANT DIFFERENCE between CONCENTRATION 4 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A S1GNIFI CANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE
D-64
0.00 0.02 0.01
-------
0.42 0.23 0.64
i.OO 1.00 1.00
1.00 1.00 1.00
1.00 1.00 1.00
TRANSFORMED DATA TABLE
NUMBER6WAS
USED FOR THIS SET OF DATA.
0.03
0.20
0.60
0.71
1.54
1.54
1.54
0.14
0.03
0.54
0.50
1 .54
1 .54
1 .54
0.10
0.38
0.67
0.93
1 .54
1 .54
1.54
A N 0 V A TABLE
Source DF Sum o-f Sq. Mean Sq. Calc F Tab F(0.05)
AMONG 6 7.430 1.238 102.990 2.850
WITHIN 14 0.168 0.012
TOTAL 20 7.598
D-65
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88189 Sample ID: Ward Cove- 88390104
(Primary 002A)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue , ... ,,.._.
Seattle, WA 98101
Bioassay Specifications v-'° . _
W)v o:'i9g
Effluent Type: Final, pulp mill effluent /Tr, ,,.,,,,
Test Type/Duration: Chronic/Bivalve Larvae - 48 hours ""' ' ~: ;;' • " ' • ••' ••'• •..-•
•• L;''! '"U.:'Jfv :"•
Test Organism; common name: Pacific oyster
scientific name: Crassostrea gigas
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/24/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: <4.8% LOEC: 4.8%
Calculation Method: LC5Q/EC50: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: 24.3%
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptability requirements met: no - control mortality should be less than 20%
Test Organisms Data
Organism Source: Brood stock from Brezina and Associates, Dillon Beach, CA
Acclimation Period: >14 days Acclimation Mortality: 0
Organism Age at beginning of Test: larvae less than 3 hours
D-66
-------
Test Desin
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
3
25 larvae per ml
100ml
EPA X personnel
composite
9/20/88
48 hours
Alaska Airlines
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45(im
Test Certification
Laboratory Manager:
Date: y
Analyst:
Date:
Barry Snyder
Alan Monji
~^ /
D-67
-------
WESTEC Bioassay Laboratory
Bivalve Larvae Bioassay Summary
WBLTest* 88189
cone. rep. # normal # abnormal
Sample ID. # 88390104 (Primary 002
total % abnormal avg. abnormal % survival
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
r
a
b
c
142
191
175
1 16
179
210
9 1
1 9
51
0
4
0
0
0
0
0
0
0
0
0
0
0
2
1
4
0
1 4
43
41
1 7
27
1 7
42
0
0
0
0
0
0
0
0
0
142
1 93
176
120
179
224
134
60
68
27
21
42
0
0
0
0
0
0
0
0
0
0.0%
2.0%
1 .0%
4.0%
0.0%
14.0%
43.0%
41.0%
17.0%
27.0%
17.0%
42.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
1.0%
6.0%
33.7%
28.7%
0.0%
0.0%
0.0%
75.7%
77.5%
38.8%
13.3%
0.0%
0.0%
0.0%
D-68
-------
PEUISIQN 1 00 5/1/87
ANoOA-UITH THE DUNNETT COMPARISON OF EACH CONCENTRATION
MEAN VS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES
A ONE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
NUMBER TRANSFORMATION
0 NONE
1 SQUARE ROOT(X)
3 LOG10/2
6 ARCSIN«X>"0.5>
NOTE :
THE LIMITS OF SIN ARE +/- 1.
7 ALGAL ANALYSIS - NO TRANSFORMATION
RESULTS OF SEQUENTIAL COMPARISONS
USING THE DUNNETT'S TEST
CALCULATED DUNNETT T VALUES
CONCENTRATION CrtLC DUNNETT T
2 -1.312028
3 -5.969578
4 -5.362445
5 -16.59972
6 -16.59972
7 -16.59972
TABULATED DUNNETT T = 2.53
NOTE: H TABULATED DUNNETT T is greater than the
CALCULATED DUNNETT T, THERE IS NO SIGNIFICANT
DIFFERENCE between that CONCENTRATION and the
CONTROL. H the TABULATED DUNNETT T is less
than the CALCULATED DUNNETT T, then THERE IS
SIGNIFICANT DIFFERENCE between that CONCEN-
TRATION and the CONTROL.
For- this set o-f data, the MINIMUM SIGNIFICANT DIFFERENCE is -0.008.
This represents a-80.3X reduction o-f the mean response -from the CONTROL,
There is NO SIGNIFICANT DIFFERENCE between CONCENTRATION 2 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 3 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 =md CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and COhr
:3 A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE
-------
0.43 0.41 0.17
0.27 0.17 0.42
1.00 1.00 1.00
1.00 1.00 1.00
1.00 1.00 1.00
TRANSFORMED DATA TABLE
" NOTETTRANSFORMATlON NUMBER~
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88190 Sample ID: Ward Cove - 88390107
(Secondary 002B)
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101
Bioassay Specifications
Effluent Type: Final, pulp mill effluent
Test Type/Duration: Chronic/Bivalve Larvae - 48 hours
Test Organism; common name: Pacific oyster
scientific name: Crassostrea gigas
Summary of Final Results
Test Starting Date: 9/22/88 Completion Date: 9/24/88
LC50/EC50: N/A 95% confidence intervals: N/A
NOEC: <4.8% LOEC: 4.8%
Calculation Method: LCso/ECsQ: N/A
NOEC/LOEC: Dunnett's Procedure
Quality Control Summary
Control Mortality: 24.3%
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: no - control mortality should be less than 20%
Test Organisms Data
Organism Source: Brood stock from Brezina and Associates, Dillon Beach, CA
Acclimation Period: > 14 days Acclimation Mortality: 0
Organism Age at beginning of Test: larvae less than 3 hours
D-71
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
5
3
25 larvae per ml
100ml
EPA X personnel
composite
9/20/88
48 hours
Alaska Airlines
WBL seawater collected from Mission Bay at
Hubbs Research Center
filtered to 0.45p.m
Test Certification
Laboratory Manager
Date: /
Analyst:
Date: /
Barry Snyder
Alan Monji
D-72
-------
WESTEC Bioassay Laboratory
Bivalve Larvae Bioassay Summary
WBLTest* 88190 Sample ID. # 88390107 (Secondary 002
cone. rep. # normal # abnormal total % abnormal avg. abnormal % survival
control
salinity
control
4.8%
9.6%
19.2%
38.5%
76.9%
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
a
b
c
142
191
175
1 16
179
210
149
73
91
0
0
0
0
0
0
0
0
0
0
0
0
0
2
1
4
0
1 4
61
43
29
0
0
0
0
0
0
0
0
0
0
0
0
142
1 93
176
1 20
179
224
210
1 16
1 20
0
0
0
0
0
0
0
0
0
0
0
0
0.0%
2.0%
1 .0%
4.0%
0.0%
14.0%
61.0%
43.0%
29.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.0%
1.0%
6.0%
44.3%
75.7%
77.5%
66.1%
0.0% 0.0%
0.0% 0.0%
0.0% 0.0%
0.0%
0.0%
D-73
-------
REVISION 1.00 5/1/87
ANOVA-WITH THE DUNNETT COMPARISON OF EACH CONCENTRATION
MEAN VS THE CONTROL MEAN. UP TO SEVEN CONCENTRATIONS
(INCLUDING THE CONTROL) AND UP TO TEN REPLICATES
MAY BE USED.
A ONE SIDED 'T' AT 95X IS ASSUMED IN ALL CASES.
THE TRANSFORMATIONS WHICH MAY BE USED ON THE DATA ARE:
NUMBER TRANSFORMATION
0 NONE
1 SQUARE ROOT(X)
3 LOGIO(X)
5
NOTE :
THE LIMITS OF SIN ARE +/- 1.
7 ALGAL ANALYSIS - NO TRANSFORMATION
RESULTS OF SEQUENTIAL COMPARISONS
USING THE DUNNETT' S TEST
CALCULATED DUNNETT T VALUES
CONCENTRATION CALC DUNNETT T
2 -1.504011
3 -8.357164
4 -19.02868
5 -19.02868
6 -19.02868
7 -19.02868
TABULATED DUNNETT T = 2.53
NOTE: If TABULATED DUNNETT T is greater than the
CALCULATED DUNNETT T, THERE IS NO S1GNIF1 CANT
DIFFERENCE between that CONCENTRATION and the
CONTROL. If the TABULATED DUNNETT T is less
than the CALCULATED DUNNETT T, then THERE IS
SIGNIFICANT DIFFERENCE between that CONCEN-
TRATION and the CONTROL.
For this set of data, the MINIMUM SIGNIFICANT DIFFERENCE is -0.002
This represents a-16.47. reduction o-f the mean response from the CONTROL
There is NO SIGNIF1 CANT DIFFERENCE between CONCENTRATION 2 and CONT
= >There IS A SIGNIFICANT DIFFERENCE between CONCENTRATI Of4 3 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 4 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 5 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 6 and CONT
=>There IS A SIGNIFICANT DIFFERENCE between CONCENTRATION 7 and CONT
INPUT DATA TABLE D_74
-------
0.61
1.00
1.00
1.00
1.00
0.43
1.00
1.00
1.00
1.00
0.29
1.00
1.00
1.00
1 .00
TRANSFORMED DATA TABLE
' NOTETTRANSFORMATlON NUMBER~
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88191 Sample ID: Ward Cove - 0118
Facility Name: Ward Cove, Alaska
Facility Location: c/oEPAX
1200 6th Avenue .•'- ;: :'-" •'•'''"...
Seattle,WA 98101 ;'; ': - -
Bioassav Specifications ^OV 0 ,'; jQp
Test Material: sediment ; • '-:i ;•'; ,t r :; ,
Test Type/Duration: Suspended Paniculate Phase, static acute - 96 hours' •' ! • n
Test Organism; common name: mysid shrimp
scientific name: Acanthomysis sculpta
Summary of Final Results
Test Starting Date: 9/29/88 Completion Date: 10/3/88
LC50/EC50: >100% elutriate 95% confidence intervals: N/A
NOEC: N/A LOEC: N/A
Calculation Method: LCsQ/ECso: N/A
NOEC/LOEC: N/A
Quality Control Summary
Control Mortality: 11.7%
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control mortality less than 20%
Test Organisms Data
Organism Source: Brezina and Associates, Dillon Beach, CA
Acclimation Period: 48 hours Acclimation Mortality: <10.0%
Organism Age at beginning of Test: juvenile
D-76
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Mortality Data (percent mortality)
Test Concentration rep.
3
3
20
2 liters
EPA X personnel
grab
9/20/88
9 days
Alaska Airlines
WBL laboratory grade saltwater
filtered to 20 |im and aerated
% Mortality
control
10.0%
- 50.0%
100%
a
b
c
a
b
c
a
b
c
a
b
c
15
10
10
0
10
20
20
25
20
40
40
10
Avg. Mortality (%)
11.7
10.0
21.7
30.0
Test Certification
Laboratory Manager:
Date:
Analyst:
Date:
: £)Cl>/l/u7\ 9*^^.Barry Snyder
i-> 'J
Alan Monji
D-77
-------
DATA SHEET FOR EFFLUENT TOXICITY TEST.
., .
Toicanl
c&t oiid
' v
AM,...
3 AC*. -M-f ^ /J~~
D/)in Pkdi?$t,(A
llllutM Stilal Numb
HTDtS Ptmiit Numbi
Cone.
CH
n
tttflbvl
j00iQ
6~0°h
100%
Ttti
C oniainer
Numbei
a
b
c
a.
fc
c
a
b
a
^
b
c
^^ V!T &&l^l
Number of
liv t Organisms
0
^0
1>v
10
10
10
10
1$
•10
^Q
v>
U)
w
24
48
'
72
SC
7
rf
•S
\tp
is-
/<«
$
i^
/9
B t glnn Ing:
Ending:
It it Orginl
Ttti Tempt
Diisolvcd ORYQ<
U
02-
S2-
72
$ |
g /
8-1
^•/
$(
/?/
8-J
i?2-
^^
#3
#}
96
,r.
^
40-
^
iii
Salinity (ppt)
0
iL
It
H
H
i
!
24
j9
?4
H
54-
41
"\S"
^
35"
3?
72
T
IS"
If
7f
6
rr
Ji,'
3»"1
*r
out
im
' ' .. Tim. '
/(?/? TiT. f ft™
n~CUvi-t-H£)tnlf}>s SCU/prt
•luit n«n
1 /) f 5 «7f
gt Xf-* ^ »- .
i
Conductivity *
0
24
48
72
96
Temperature ( C)
0
W
c,./
/«,.J
/c/t
/1-4
/^4
/H
iVi
/tj»
^•f
^4
24
|-f
&»•*
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zo-f
^.1
48
IH
/VJ
1-2-
IW
^f-
KM
\\-i
H,.,
ho
/
ft-'
/
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88192 Sample ID: Ward Cove- 0119
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101
Bioassay Specifications
Test Material: sediment
Test Type/Duration: Suspended Paniculate Phase, static acute - 96 hours
Test Organism; common name: mysid shrimp
scientific name: Acanthomysls sculpta
Summary of Final Results
Test Starting Date: 9/29/88 Completion Date: 10/3/88
LC50/EC50: >100% elutriate 95% confidence intervals: N/A
NOEC: N/A LOEC: N/A
Calculation Method: LCsQ/ECsQ: N/A
NOEC/LOEC: N/A
Quality Control Summary
Control Mortali ty : 11.7%
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control mortality less than 20%
Test Organisms Data
Organism Source: Brezina and Associates, Dillon Beach, CA
Acclimation Period: 48 hours Acclimation Mortality: <10.0%
Organism Age at beginning of Test: juvenile
D-79
-------
Test Desien
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Mortality Data (percent mortality)
Test Concentration
control
10.0%
- 50.0%
100%
rep.
a
b
c
a
b
c
a
b
c
a
b
c
3
3
20
2 liters
EPA X personnel
grab
9/20/88
9 days
Alaska Airlines
WBL laboratory grade saltwater
filtered to 20 Jim and aerated
% Mortality
15
10
10
5
25
10
5
20
20
15
10
30
Avg. Mortality
11.7
13.3
15.0
18.3
Test Certification
Laboratory Manager:
Date: /
Analyst:
Date: /' A A*
^<-^>£^fearry Snyder
Alan Monji
7
D-80
-------
Oe
•!...«.«.
DATA SHEET FOR EFFLUENT TOXICITY TEST.
CMC Qlt
io
^o
>o
J6
w
w
10
U>
M>
24
48
,»
72
3G
'I
Jj
W
W
h
/S"
/ft
T^1
14
i'*'
n
)7?
H
Oil Jolvtd Oiygtn
Img/l)
0
8t
8-1
e-fc
17
7--B
7-il
77
7-1?
7-7
72,
n
77
24
74
7-i
73
/l
n
7Z
7i
11
11
70
70
71
48
Tl
7-1
7-o
H
?t
7-1
/I
/•i
7-1
n
7-1
7.0
72
71
7t
li>
7.i?
7-0
7?
7<>
7-f
7-1
70
7-<
7-1
9G
A^J
•7-6)
7-'
7r?
7-a-
?,>•
7 '
7.1
7-1-
7- J
7, /
?-7-
pM
0
81
V°
%»
BL
%l
9>-t
fri
^
«•«
go
B-o-
8-3
24
80
8'
f-
6'
8-i
fl-i
^
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8-1
80
^J
46
ao
e°
&
fri
8-i
«i
«-3
ei
8-0
Si
01
fi-o
72
?i
«•«
8/
8i
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8'
g.t
&/
8-0
^1
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96
/•'I
tli
ijj
ff'l
ff:>
•^.(
2s
?-i
a. J
ia.
ST
&••{
Salinity (ppt)
0
3r
ii
H
7^
i
!
2<
3T
vf
H
^
48
3r
3^"
^
^
72
3r
^c
?>"
5^
96
3i>
36
^
3t
i
Conductivity *
0
24
48
72
96
Temperature ( C)
0
IU
rt-»
^3
/H
ii/
y^
\W
M
IV
n-s
\w
M
24
M
lW
itf
IU
/9-^
/
-------
BIOMONITORING REPORT FORM
WESTEC SERVICES BIOASSAY LABORATORY
10477 C Roselle Street
San Diego, CA 92121
(619) 458-1644
WBLTestNo.: 88193 Sample ID: Ward Cove - 0121
Facility Name: Ward Cove, Alaska
Facility Location: c/o EPA X
1200 6th Avenue
Seattle, WA 98101
Bioassay Specifications
Test Material: sediment
Test Type/Duration: Suspended Paniculate Phase, static acute - 96 hours
Test Organism; common name: mysid shrimp
scientific name: Acanthomysis sculpta
Summary of Final Results
Test Starting Date: 9/29/88 Completion Date: 10/3/88
LC50/EC50: 40.0% elutriate 95% confidence intervals: 31.5% 50.8%
NOEC: N/A LOEC: N/A
Calculation Method: LC5Q/EC50: Moving Average
NOEC/LOEC: N/A
Quality Control Summary
Control Mortality: 11.7%
Temperature maintained: yes
Dissolved oxygen maintained above minimum for test type: yes
All test acceptibility requirements met: yes - control mortality less than 20%
Test Organisms Data
Organism Source: Brezina and Associates, Dillon Beach, CA
Acclimation Period: 48 hours Acclimation Mortality: <10.0%
Organism Age at beginning of Test: j uvenile
D-82
-------
DATA SHEET FOR EFFLUENT TOXICITY TEST.
. tufr
Beginning: n«i«
Tlm«
ClllutM Stilil Numbti
NPOCS Ptrrall Numbti
U)t>Ltt
Ending: n.i. J^/O/S T'™«
Te.i n.n •"'••- frcbn-tttenuj
Ted Tempenlui* Ring*.
J.Q
Cooc.
CH
S
(JPfl/>W|
10%
S0°lo
/^°/o
Tin
Conliinei
Numbei
a.
b
C
a
j)
C
<*
1)
c
a
b
c
Number ol
live Organijmi
0
20
ZO
W
20
10
10
W>
IX)
10
10
00
Jo
24
48
1
72
90
17,,
7/
nf
^
^
t?-
e
9
*y
3
d
Dissolved 0»yg«n
(mg/l)
0
6-z
to
8-t
7-^
7-9
7^
(v/
t-?
6-9
7/
7-'
^
24
74
72
7-5
7l
70
7-o
7/
7.0
*•*
^>5
67
(,5
48
72
7-i
70
7f
7D
fr<
72
7-1
71.
7i
7-
^^
72
^•^
^^
71
TO
?/
70
7-<
'•?
7-f
?'0
?./
71
96
7-t>
7,^
7-1
?.x
7-p
T.X
?i
7-i
7,^
7.)
?.o
?•/
pll
0
8-1
£0
fl°
81
fH
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fl°
6-1
61
61
g«
8-0
24
go
0,1
6)
81
8-1
^>
&>
B-4
»,i
64
fts"
§4
48
80
&o
80
fi-i
8-1
01
?-^
8-1
fti
B-i
8-'
B3
72
go
9-1
?'
?/
fi-/
/f'
t>)
8)
t*
Vi
&y
^}
96
7-'(
?•(?
?.o
?•*
S. 1
?(•>-
*•>
83
fr''
S^
*-V
1-5^
Salinity (ppt)
0
3^
34
H
34
1
1
'
1
24
3^
54
H
^
41
^
3^
^<
V
72
3T
^
3<
^
96
v,
1<
K!
tf
i
Conductivity *
0
24
48
72
96
Temperature ( C)
0
IW
A-'
/W
^
/f4
»H
rt-5
IU
!<}.J
/U
rt-<"
IH
24
|H
/^
/?9
201
70-3
201
102
ID.?
70-2.
V*
zar
Z»2^
48
^*
*;
^
w
IS-i
I«H)
Kfi
I1.i
fr3
l^f
frf
(<;-<
72
M-'
«•;
fty
h-f
fi.o
iVi
R-l
/i-0
fry
ll-i
d./
Ci-»
96
11ft
'vTr
•^•7
IW
(V#
M-7
ti-7
Cr.7
|CT-'-
fr.t
/7-7
fr.t
o
I
oo
•^Conductivity values X 10~
-------
Test Design
Number of Effluent Concentration:
Number of replicates/test concentration:
Number of Organisms per replicate:
Volume of test material per chamber:
Effluent Collection
Effluent Collector:
Type of sample (eg. grab, compsite):
Collection Dates:
Maximum Holding Time:
Method of Shipping:
Dilution Water
Dilution Water Source:
Method of Preparation:
Mortality Data (percent mortality')
Test Concentration rep.
3
3
20
2 liters
EPA X personnel
grab
9/20/88
9 days
Alaska Airlines
WBL laboratory grade saltwater
filtered to 20 |im and aerated
% Mortality
control
10.0%
- 50.0%
100%
— ' a.
a
b
c
a
b
c
a
b
c
a
b
c
15
10
10
10
20
25
40
35
60
80
85
100
Avg. Mortality
11.7
18.3
45.0
88.3
Test Certification
Laboratory Manager: /ji
Date: H/1/0&
Analyst:
Date: I'/-* <^
-t^Barry Snyder
Alan Monji
/f JLi-~-^. I
D-84
-------
88 W
NUMBER NUMBER PERCENT BINOMIAU l^O^oC (
EXPOSED DEAD DEAD PROB.OO
!00 60 53 88.33334 3.835959E-08 /H^j j
50 60 27 45 3.785926
10 60 11 18.33333 3.78064E-05 C1) \
THE BINOMIAL TEST SHOWS THAT 10 AND 100 CAN BE
USED AS STATISCALLY SOUND CONSERVATIVE 95 PERCENT
CONFIDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL
ASSOCIATED WITH THESE LIMITS IS 99.99996 PERCENT.
AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 53.71409
»»»»RESULTS CALCULATED USING THE MOVING AVERAGE METHOD
SPAN G LC50 95 PERCENT CONFIDENCE LIMITS
2 5.444088E-02 39.99059 31.49181 50.81626
»>»»RESULTS CALCULATED USING THE PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT PROBABILITY
4 30.20642 9.220016 2.393305E-03
SINCE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED
USING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED.
SLOPE = 1.861044
95 PERCENT CONFIDENCE LIMITS =-8.367324 AND 12.08941
LC50 = 37.04299
95 PERCENT CONFIDENCE LIMITS = 0 AND + INFINITY
LCI = 2.081832
95 PERCENT CONFIDENCE LIMITS = 0 AND + INFINITY
D-85
-------
APPENDIX E
INTERCALIBRATION DATA
-------
Table E-l. Intercalibration Results
M
I
Station
KLI 5-1
ADEC 61
KPC 48
Station
KLI 5-2
ADEC 62
KPC
Station
KLI 5-3
ADEC 63
KPC
Station
KLI 5-4
ADEC 64
KPC 49
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Temperature
KLI ADEC
14.45 15
ND 14.5
13.27 13
12.0 12
Temperature
KLI ADEC
14.31 14.5
14.32 14.5
13.36 13
11.72 12
Temperature
KLI ADEC
14.36 14.5
14.36 14.5
13.65 14
11.87 12
Temperature
KLI ADEC
14.27 14.5
14.26 14.5
13.36 13.5
12.14 12
KPC
ND
ND
ND
ND
KPC
ND
ND
ND
ND
KPC
ND
ND
ND
ND
KPC
ND
ND
ND
ND
Salinity
KLI ADEC
23.73 23
ND 23.5
26.17 26
27.85 ND
Salinity
KLI ADEC
24.2 24
24.2 24
26.03 26
28.25 ND
Salinity
KLI ADEC
24.11 23.5
24.12 23.5
25.57 25
28.03 ND
Salinity
KLI ADEC
23.87 23.5
24.10 23.5
26.08 26
27.88 ND
KPC
ND
ND
ND
ND
KPC
ND
ND
ND
ND
KPC
ND
ND
ND
ND
KPC
ND
ND
ND
ND
Dissolved Oxygen
KLI ADEC KPC
7.59 4.4 ND
ND 5.5 ND
7.32 6.9 ND
7.58 6.1 ND
Dissolved Oxygen
KLI ADEC KPC
8.38 6.5 ND
8.26 6.5 ND
8.07 6.9 ND
8.01 7.9 ND
Dissolved Oxygen
KLI ADEC KPC
7.79 6.6 ND
7.77 6.1 ND
7.71 7.1 ND
7.75 7 ND
Dissolved Oxygen
KLI ADEC KPC
8.65 7 ND
8.56 6.4 ND
8.15 6.6 ND
8.1 6.3 ND
-------
Table E-l, cont.
I
NJ
Station
KLI 5-5
ADEC 65
KPC
Station
KLI 4-1
ADEC 41
KPC 14
Station
KLI 4-2
ADEC 42
KPC 15
Station
KLI 4-3
ADEC 43
KPC 16
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
KLI
14.17
14.13
ND
12.10
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
14.81
14.75
13.32
12.28
Temperature
ADEC
14.5
14.5
13.5
12
Temperature
ADEC
15.5
15
13.5
12.5
Temperature
ADEC
15.5
14.5
14
13
Temperature
ADEC
14.5
14.5
14
13
-
KPC
ND
ND
ND
ND
KPC
16.5
15.9
14.7
12.9
KPC
16. 1
16.1
15.2
13 .2
KPC
16.3
15.4
15
13.4
Salinity
KLI ADEC
24.12 23
24.24 23.5
ND 26
28 ND
Salinity
KLI ADEC
ND 22.5
ND 22.5
ND 25
ND ND
Salinity
KLI ADEC
ND 22
ND 23.5
ND 25
ND ND
Salinity
KLI ADEC
23.67 23.5
23.76 23.5
26.13 24
27.6 ND
KPC
ND
ND
ND
ND
KPC
24.2
25.3
27.3
29.4
Dissol
KLI
8.48
8.24
ND
7.86
Dissol
KLI
ND
ND
ND
ND
.ved
Oxycren
ADEC KPC
6.6
6.4
6.3
6.3
.ved
ADEC
5.7
6.4
8.3
7.1
Dissolved
KPC
24.4
24.3
24.2
29.1
KLI
ND
ND
ND
ND
ADEC
6.4
9.4
8.4
8.5
Dissolved
KPC
25.1
25.3
25.4
27.5
KLI
7.57
7.44
7.07
7.26
ADEC
8.4
9.2
8.5
8.1
ND
ND
ND
ND
Oxygen
KPC
5.3
5.3
7.2
4.7
Oxygen
KPC
5.4
5.4
6.4
4.6
Oxygen
KPC
7.5
7.6
6.7
4.6
-------
Table E-l, cont.
M
I
u>
Station
KLI 3-1
ADEC 31
KPC 9
Station
KLI 3-2
ADEC 32
KPC 10
Station
KLI 3-5
ADEC 35
KPC 13
Station
KLI 2-2
ADEC 22
KPC 5
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
10 m
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
Temperature
ADEC
16
15
14
13
Temperature
ADEC
16
15.5
14
12.5
Temperature
ADEC
15.5
15
14.5
ND
Temperature
ADEC
15
15
14
12.5
"•
KPC
16.8
16.1
14.5
13.6
KPC
16.5
16. 1
15.4
13.4
KPC
17.9
15.6
15.2
13.6
KPC
16.8
15.9
15.2
14.5
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
Salinity
ADEC
22
23
24.5
ND
Salinity
ADEC
22
22
24
ND
Salinity
ADEC
22.5
23
23.5
ND
Salinity
ADEC
22.5
22.5
24
ND
Dissolved
KPC
25.
25.
27.
27.
1
2
1
3
KLI
ND
ND
ND
ND
Oxygen
ADEC KPC
5.7
5.7
8
5.7
Dissolved
KPC
24.
24.
25.
29.
7
5
6
1
KLI
ND
ND
ND
ND
6.0
5.9
7
7.2
Oxygen
ADEC KPC
6.2
5.7
7.2
6.6
Dissolved
KPC
25.
25.
26.
30.
1
2
1
1
KLI
ND
ND
ND
ND
ADEC
4.5
5
6
ND
Dissolved
KPC
23.
24.
25.
27.
4
3
2
2
KLI
ND
ND
ND
ND
ADEC
6.5
6.1
7.7
6.9
5.2
5.1
6.1
5.0
Oxygen
KPC
5.7
6.0
6.5
5.4
Oxygen
KPC
5.4
5.1
6.1
4.5
-------
Table E-l, cont.
M
I
Station
KLI 2-3
ADEC 23
KPC 6
Station
KLI 2-4
ADEC 24
KPC 7
Station
KLI 50
ADEC 50
KPC 50
Station
KLI 51
ADEC 51
KPC 51
Depth
sf c
1 m
5 m
7 m
Depth
sf c
1 m
5 m
8 m
Depth
sf c
1 m
5 m
7-10 m
Depth
sfc
1 m
5 m
10 m
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
14.46
14.37
13.47
12.91
KLI
14.18
14.18
12.52
12.06
Temperature
ADEC
15.5
15
14
12.5
Temperature
ADEC
15.5
15
14
12.5
Temperature
ADEC
15
14.5
14.5
12.5
Temperature
ADEC
14
14
14
13
KPC
16.2
16.8
15.9
15
KPC
16.3
15.6
14.5
20.1
KPC
15.6
15.4
15.2
14.7
KPC
15.4
15
14.5
12.5
KLI
ND
ND
ND
ND
KLI
ND
ND
ND
ND
KLI
24.04
24.17
25.92
26.72
KLI
23.88
23.89
27.13
28.56
Salinity
ADEC
21
23
24
ND
Salinity
ADEC
21
22.5
24
ND
Salinity
ADEC
23
23
24
ND
Salinity
ADEC
23.5
23.5
25
ND
KPC
23.1
24.4
25.7
28.3
KPC
25.6
25.1
27.2
23.3
KPC
26.1
25.2
25.4
26.1
KPC
25.2
25.1
26.1
29.2
Dissolved Oxygen
KLI ADEC KPC
ND 6.8 5.1
ND 6.8 5.0
ND 7.5 5.8
ND 8.2 5.2
Dissolved Oxygen
KLI ADEC KPC
ND 7.1 5.9
ND 6.6 6.2
ND 7.9 7.2
ND 7.5 5.0
Dissolved Oxygen
KLI ADEC KPC
8.32 8.6 7.3
8.25 9 7.4
8.15 10.1 7.4
8.25 9.1 7.3
Dissolved Oxygen
KLI ADEC KPC
7.96 9.7 7.8
7.5 9.8 7.9
6.86 9.7 7.4
6.59 9.4 5.5
-------
Table E-l, cont.
Station
KLI 2-5
ADEC 25
KPC 8
Station
KLI 1-1
ADEC 11
KPC
Station
KLI 1-2
ADEC 12
KPC
Station
KLI 1-3
ADEC 13
KPC
Depth
sfc
1 m
5 m
10 m
Depth
sfc
1 m
5 m
b
Depth
sfc
1 m
5 m
b
Depth
sfc
1 m
b
KLI
12.16
15.2
13.03
12.2
KLI
14.87
14.86
13
11.85
KLI
14.88
14.73
12.88
11.96
KLI
14.81
14.75
13.98
Temperature
ADEC
16
15.5
14
12.5
Temperature
ADEC
15
15
13
12
Temperature
ADEC
15.5
15.5
13.5
ND
Temperature
ADEC
15.1
15
ND
KPC
16.0
15.4
14.5
20.9
KPC
14
14
ND
13.3
KPC
14.0
14
13.2
ND
KPC
14
12
ND
Salinity
KLI ADEC
27.44 21
22.37 22
25.97 25
27.35 ND
Salinity
KLI ADEC
13.35 17
23.35 23
26.1 25
28.09 ND
Salinity
KLI ADEC
5.61 7
23.53 20
26.31 23
28.01 ND
Salinity
KLI ADEC
17.2 20.5
23.7 23
24.41 ND
KPC
24.8
25.1
27.2
22.1
KPC
8.2
25.1
ND
28.3
KPC
9.0
26.4
28.5
ND
KPC
25.2
24.4
ND
Dissolved Oxygen
KLI ADEC KPC
5.86 5 5.6
4.84 5.3 6.5
5.7 6.7 7.0
5.99 6.5 5.5
Dissolved Oxygen
KLI ADEC KPC
6.41 5.7 8.2
4.95 5.7 5.1
5.46 6.2 ND
0.25 0.5 .9
Dissolved Oxygen
KLI ADEC KPC
8.74 8.8 7.7
5.48 5.2 6.4
5.47 6 5.4
0.25 ND ND
Dissolved Oxygen
KLI ADEC KPC
8.21 5.4 6.5
5.1 5.3 5.4
5.75 ND ND
-------
Table E-2. Intercalibration Sampling Matrix August 21, 1988
Station
50
51
5-1
5-2
5-3
5-4
5-5
4-1
4-2
4-3
3-1
3-2
3-3
3-4
3-5
2-1
2-2
2-3
2-4
2-5
1-1
1-2
1-3
KLI
11:25
11:10
11:45
12:00
12:10
12:25
12:35
-
-
12:45
-
-
-
-
-
-
-
-
-
18:25
19:15
19:45
20:00
KPC
11:25
11:10
NS
NS
NS
NS
NS
12: xx
12: xx
12:45
13: xx
13: xx
13: xx
13 :xx
15:00
15: xx
15:40
16:00
15:20
18:25
19:15
19:45
20:00
ADEC
16:35
16:45
11:35 (T,
11:45 (T,
12:05 (T,
12:20 (T,
12:30 (T,
17:45
17:10
17:00
17:20
17:35
17:45
-
15:00
-
15:40
16:00
15:20
18:25
19:15
19:45
20:00
S)
S)
S)
S)
S)
xx - Exact Time not available
NS - No Sampling
(T,S) - Valid data for temperature and salinity only
E-6
-------
APPENDIX F
EPA ENVIRONMENTAL RESEARCH LABORATORY
(DULUTH) DIOXIN STUDY
-------
NDS PHASE II: 5I04CCUMULATIVE
Sample "Tracking System
EPISODE s
3245
SCC #:
\[\\ '••'•' ; {,~:*f.c."-'-'sS?!ff"™
U Vl , ,', p .v"-:V--10:QQ';;-
... %- 'J' I" • .,-._ •-/•
. ERL-D 'Lpc
OJ02362A : '•• .'"••
Sampling Information:
Sampling Office: U.S. FISH AND WILDLIFE SERVICE
Stste i City: £ K JUNEAU
Sampling Contact: EVERETT ROr I MS ON ~ ^J^^OK; tp^
Oata Sampled: 5/13/37
Sit.2 Location: i K WARD COVE NEAR KITCHIKAN
552S'^5" Longitude:
Date Recei
Rerun:
I
" ""1
LetiTuds: N
Analysis Lc-,t>:
.•Utnx Type:
Sa.-nple Composite:
W 131
ved: 1
' 44V20"
1/11/87.
fincllytic^l:
extraction Dat?
G C / # 5 I J
Lie I J
.-/eight
%'- i c i d
-P£ InciCction
2/ 2/? ?
••' A T i 5 0 4 5 0
G 0 2 0 2 8 c C S
20.0?
4.2
Pesticide & Industrial Chemicals
O/ O/ 0
.BSE lipic on GPC:
0.00
0.0
0.000
Comments :
F-l
-------
NDS PHASE ii: BIOACCUMULATIVE POLLUTANT STUDY
EPISCQc.a: 3245 SCC *: DJ023624 ERL-D Loc.: 235 '
DATA for al0 SIGN ICICANT PCLYCHLORIN ATEO DI5ENZODIDXINS AND FURANS: '
Analyte CfiS No.: Ion Qatio S/N S&REC Det. Lim. Amo'untCpg/g)
2,3
2,2
3,4
2,3
1 ,2
2,3
2,3
1 , -
1,2
1 ,2
1,2
2 , :.
i,:
1,2
1,2
1,2
1,2
,7,5- TCDF
,o,7- TCOF
,6,7- TCO=
,7,d- TCDD
,5,7,i- ? e C D F
,4,7,3- P e C 5 = #
,-,o,7- P '- C D F
,^,7,-i- r 3 C J _
,3, ,.6, 7- HxCDF ,=#
, 5 ,-»,?, 8 - -i x C 0 F *
, 3 , e , 7 , o - r, x L 3 =
, a , 6 , 7 , 3 - -• x C D F "#
, j , 7 , 6 , ? - - x C D .-
,5,4,7,8- nxCDD
,3,6,7,5- n x C D D
,3,7,3,9- rlxCDD
, 3 , t , 6 , 7 , 3 - h j C D F -=
51207-31-9 0.
17-.6-01-S 0.
57117-41-5 2.
5 7117-31-4 0.
0.
40311-76-4 2.
7JD4S-23-9 0.
Q _
19405-74-3
f 7 -5 3-5 5-7 0.
^7: £-2-39-4 0 .
55.C-72-39-7 0.
i7-: 71-00-- D.
32598-13-3 0.
1.
0.
0.
95
00
54
58
00
46
00
^
00
00
CO
00
00
04
00
s 5
niiWv^s-^o* o.oo
-U2
.". •
V •
,3»4,6i7»8- HoGOD
Coeiute s with 123
Indication of OPE
0..9.7,.
478 rlxCOF on D35
interfersnce at.
30
this
1.
1.
1.
1.
1.
1.
1.
** •
1.
1 .
1.
1 .
1.
1.
1.
1
1.
22.
00
00
00
00
00
00
00
77
00
00
00
00
00
00
00
00
00
,10
80
80
80
84
82
82
32
89
35
85
85
85
89-
39 .
39
75
-J-5
77
1.
0.
u.
•1.
0.
1.
0.
0.
2.
2.
1.
2.
- -:>2.
". :: "-"^ *
— n
i.
,2.
&$
2500
49
00
4900
1.1
77
08
82
00
83
84
00 .
00
00
00
00
00
00
9600
77
00
4.600
01
rS p
45
Al
00
o n :
00
0.0.,,
i.OB
ND
NO
ND
ND
ND
ND
ND
(
ND
ND
ND
ND
ND
ND
:V NO
ND
L^.*JiP
'ispmer .
Toxicity Equivalency ConcentrationJ
Percent Contribution of 23.78"TCDF»
2378 TCDD and 12378 PeCDD to total
T£F value: '9,9,
0.24
F-2
-------
EPISODE #.
NDS PHASE II: 8IOACCUMULATIVE POLLUTANT STUDY
Sample Tracking System ERL-D
3245
SCC *'
DJ023624
Loc
235
Sampling Information:
Sampling Office: U.S. FISH
State L City: AK. JUNEAU
Sampling Contact: EVERETT ROBINSON-uj
Date Sampled: 6/18/87
Site Location: AK WARD COVE NEAR
Latitude: N 55 23'45"
Analysis Lab: D
Matrix Type: F W3 FLATHEAD
Sample Composite: 10
AND WILDLIFE SERVICE
SOLE
Longitude: W 131 44'20M
Date Received: 11/11/87
Species Code:
Analytical:
Extraction Date:
GC/MS ID:
LAB ID:
Weight:
%Lipid
DPE Indication
PCDD/PCDF Pesticide I Industrial Chemicals
2/ 2/88 9/21/88-
MAT880450 DR88868
G020288CS J092188JJ
20.03 20.07
4.2 3.9
Y Mass lipid on GPC: 0.790
Comments:
F-3
-------
tPlSODE #: 3245
SCC *: DJ023624
ERf-D Loc.:
235
Target Analyte
I»3f5- Trichlorobenzene
1»2»4- Trichlorobenzene
1»2»3- Trichlorobenzene
Hexachlorobutadiene
1,2,4,5- Tetrachlorobenzene
1*2,3»5- Tetrachlorobenzene
Siphenyl
1,2,3,4- Tetrachlorobenzene
Pentachlorobenzene
Trifluralin
Alpha-BHC
Hexachlorobenzene
Pentachloroanisole
Gamma-BHC CLindane)
Pentachloronitrobenzene
Diphenyl Disulfide
Heptachlor
Chlorpyrifos
I sopropalin
Octachlorostyrene
Heptachlor epoxide
Qxychlordane
Chlordane, trans
Chlordane» cis
Nonachlor, trans
p,P '- DOE
Dieldrin
Nitrofen
End rin
Perthane
Chlorbenzilate
Nonachlor, cis
Methoxychlor
Dicofol CKelthane)
Mirex
Total Monochlorobipheny1
Total Diehlorobiphenyl
Total Trichlorobiphenyl
Total Tetrachlorofaiphsny1
Tot.al Pentachlorobiphenyl
total Hexachlorobiph.nyl
TotaJL Heptachlorobiphenyl
T o t at" Oct ach'lo rbbi pn^rfyl:
Total Nonachlorobiphenyl
Total Oecachlorobipheny1
Jotal Polvchlorinated Biphenyls
Mercury ( AA analysis)
CASRN
108-70-3
120-82-1
87-61-6
87-68-3
95-94-3
634-90-2
92-52-4
634-66-2
608-93-5
1582-09-8
319-84-6
118-74-1
1825-21-4
58-89-9
82-68-8
882-33-7
76-44-8
2921-88-2
33820-53-0
29082-74-4
1024-57-3
26880-48-8
5103-74-2
5103-71-9
39765-80-5
72-55-9
60-57-1
1836-75-5
72-20-8
72-56-0
510-15-6
3734-49-4
72-43-5
115-32-2
2385-85-5
27323-18-8
25512-42-9
25323-68-6
26914-33-0
25429-29-2
2A60.l-.64-4
28655-71-2
QA Flag
53742-07-7
2051-24-3
7439-97-6 NO
CONCN
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
ND
ND
ND
ND
NO
Cng/g)
0.27
ND
NO
ug/g
7.48
5.29
1.99
5.02
10.-1
47.3
51.3
0.85
129
Iod obenzene
lodonaphthalene
4»4'-Diiodobiphenyl
C ommen t s•
F-4
-------