REPORT ON POLLUTION OF
    THE MERRIMACK RIVER
 AND CERTAIN TRIBUTARIES


       part m- Stream Studies
              Biological
          .'  MASS.
  U.S. DEPARTMENT OF THE INTERIOR
  FEDERAL WATER POLLUTION CONTROL ADMINISTRATION
Merrimack River Project-Northeast Region
       Lawrence, Massachusetts
            August 1966

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                  REPORT ON

      POLLUTION OF THE MERRIMACK RIVER

           AND CERTAIN TRIBUTARIES

   PART III - STREAM STUDIES - BIOLOGICAL
              Warren H. Oldaker
       U. S. Department of the Interior
Federal Water Pollution Contrql Administration
               Northeast Region
            Merrimack River Project
            Lawrence, Massachusetts
                  August 1966

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                        TABLE OF CONTENTS
INTRODUCTION ... 	        1

GENERAL OBSERVATIONS 	        2

     GRADIENT  	        2
     RIVER BOTTOM	        2
OBSERVATIONS FOR SPECIFIC REACHES
     CLEAN WATER CONTROL 	       4
     FEMIGEWASSET AND WINNIPESAUKEE RIVERS 	       6
     REACH 1 (115.70 to 114.04)	       7
     REACH 2 (113.53 to 102.84)	       9
     REACH 3 (100.71 to 86.80)	      10
     REACH 4 (86.80 to 81.05)	      12
     REACH 5 (81.05 to 73.14)	      14
     REACH 6 (73.14 to 55.75)  	      15
     REACH 7 (54.80 to 49.82)	      18
     REACH 8 (49.82 to 40.60)	      19
     REACH 9 (40.60 to 28.99)	      20
     REACH 10 (28.99 to 15.70)	      21
     REACH 11 (15.70 to 0.00)	      24

BOTTOM ORGANISMS OF SOUHEGAN RIVER	      27

PRODUCTIVITY OF THE MERRIMACK RIVER	      30

MICROSCOPIC PLANKTON IN MERRIMACK RIVER  	      32

SUMMARY AND CONCLUSIONS  	      34

REFERENCES	      37

APPENDIX	      39
                             - i -

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                              LIST OF FIGURES


FIGURE NO.                                                FOLLOWS PAGE NO.

    1       Merrimack River Basin 	 .  Appendix A-15

    2       Numbers and Kinds of Benthic Organisms -
                 1964-65, Merrimack River	         4

    3       Merrimack River Tributaries, Numbers and
                 Kinds of Benthic Organisms - 1964  ...         4

    4       Distribution of Benthic Organisms in
                 Merrimack River  	         4

    5       Benthic Organisms in Merrimack River
                 Estuary - 1964	        26

    6       Distribution of Benthic Organisms in
                 Souhegan River	        28

    7       Productivity of the Merrimack River -
                 August 1965	        30

    8       Standing Crop of Plankton - 1965, Merrimack
                 River at Lawrence	        32
                                  - ii -

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                               LIST OF TABLES
TABLE NO.                                                          PAGE NO.

    1       Biological Sampling Stations and Reference Points
                 Merrimack River and Tributaries	     A-l

    2       Number of Bottom Organisms Per Square Meter  ....     A-8

    3       Kinds of Bottom Organisms in Ke'rrimack River and
                 Numbers Per Square Meter	     A-9

    4       Souhegan River Miles	     A-ll

    5       Kinds of Bottom Organisms in Souhegan River and
                 Numbers Per Square Meter	     A-12

    6       Productivity of Merrimack River - August 1965  ...     A-13

    7       Most Abundant Genera  of Algae in Merrimack River
                 April-October, 1965	     A-14

    8       Most Abundant Genera of Zooplankton in Merrimack
                 River	     A-15
                                 - iii -

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                               INTRODUCTION







          In February 1964, the U. S. Department of Health, Education,



and Welfare established the Merrimack River Project to carry out a study  ,



in the Merrimack River Basin (Figure l).   The basic objectives of the



project were twofold:





1.        Evaluation of the adequacy of the pollution abatement measures



          proposed for the Merrimack River within Massachusetts.



2.        Development of adequate data on the water quality of the



          Merrimack River and its tributaries.  Waters in both New



          Hampshire and Massachusetts were to be studied.





          As part of the study of water quality, a detailed biological



survey of the Merrimack River, extending from Franklin, New Hampshire, to



the mouth at Newburyport, Massachusetts,  was conducted during the summer



months of 1964 and 1965.  Biological surveys were also carried out on


                                                                      (1)
several tributaries, including the Souhegan River and the Nashua River   .



The primary goal of these surveys was to  evaluate the effects of municipal



and industrial wastes on the benthic fauna.
                                  - 1  -

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                          GENERAL OBSERVATIONS








GRADIENT




          Where the general nature of the stream community was con-




sidered, an estimate of the gradient or longitudinal slope was obtained




from topographic maps.  This estimate did not assess "microstratifica-




tion."




          The Merrimack River drops 263 feet in the 116 miles between




Franklin, New Hampshire, and the mouth at Newburyport, Massachusetts.




However, much of the decrease in elevation occurs at points where dams




have been constructed, resulting in a relatively gentle  slope for most




of the  length of the river.  Gradient alone then was considered insigni-




ficant  in determining the distribution of benthic fauna  except below




dams or in the specific areas mentioned for each reach.








RIVER BOTTOM




          The physical characteristics of the benthic  sediments were




based on macroscopic  examination during field biological sampling opera-




tions with the Fetersen dredge.  The  river bed may be  conveniently




divided into  six zones based on  these observations.  River miles are the




distances upstream of the U. S.  Coast Guard  light at Newburyport,




Massachusetts.   A list  of sampling stations  and key points along the




Merrimack River  and their associated  river miles is presented in Table  1




 in the Appendix.




           1.   River miles 116 to 90.   This  zone extends  from Franklin





                                   - 2 -

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    to Concord, New Hampshire,  "he benthic sediments were



    primarily composed of rock, gravel and coarse sand.



2.  River miles 90 to 65.  This zone extends from Concord to



    Goff's Falls below Manchester, New Hampshire, and has



    sediments consisting mainly of fine sand and silty loam.



3.  River miles 65 to 55.  The benthic sediments from Goff !s



    Falls to Nashua, New Hampshire, were primarily coarse



    sand and gravel.



4.  River miles 55 to 45.  This zone extends from Nashua,



    New Hampshire, to Tyngs Island, upstream of Lowell, Mass-



    achusetts.  The benthic sediments were primarily coarse



    sand and silt with some sludge build-up.



5.  River miles 45 to 2.  The benthic sediments from Tyngs



    Island to Newburyport, Massachusetts, were primarily



    sludge and silt with some sand.



6.  River miles 2 to 0.  This portion of tl e  estuary had



    sediments composed of coarse sand with some silt and



    sludge.
                        - 3  -

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                    OBSERVATIONS FOR SPECIFIC REACHES








          Data obtained in the biological survey were grouped and dis-




cussed, as nearly as possible, for reaches having similar physical




characteristics.  Eleven reaches between Franklin, New Hampshire, and




the mouth of the river were selected, plus an additional station on



each of the Winnipesaukee and Pemigewasset Rivers.  Information for a




control station above any significant waste discharge is presented in




Table 2 to show the type of relatively clean-water associated bottom




fauna that may be expected in non-polluted waters*




          The number of bottom organisms per square meter and the




various kinds of organisms found in the Herrimack River and significant




tributaries near their confluence with the Herrimack are presented in




Table 3.  This information is illustrated in Figure 2 for the Merrimack




River and in Figure 3 for the tributaries.  The biological condition




of the Herrimack River is shown in Figure 4.








CLEAN WATER CONTROL




          An assemblage of bottom organisms commonly found in clean



water stream beds (mayflies, stoneflies, caddisflies, beetles and cer-




tain midgeflies) was difficult to find in the Herrimack River Basin.




No such area was found in the Herrimack River itself*



          The principle streams and smaller tributaries were found to






                                   - 4 -

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   I  .
Poluticn Sensitive

Intermediate

Pollution Tolerant
                                                                                                                                                                                                                              . 20
                                                                                                    TO               60


                                                                                                       MERRIMACK fWVER 'MILES
                                                                                           NUMBERS AND KINDS OF BENTHIC ORGANISMS' 1964-65

                                                                                                            MEWflMACK  RIVER
                                                                                                                                                                                                                              . 10
                                                                                                                                                                                                                              . 5
                                                                                                                                                                                                                              - 16
                                                                                                                                                                                                                           ''-,   . 15
                                                                                                                                                                                                                              _ 9
                                                                                                                                                                                                                              _ e
                                                                                                                                                                                                                              _ 7
                                                                                                                                                                                                                              - 6
                                                                                                                                                                                                                              _ 4
                                                                                                                                                                                                  _ 3
                                                                                                                                                                                                                              _ Z
                                                                                                                                                                                                                         FIGURE 2

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  LEGEND'
            Pollution Sensitive

            Intermediate

            Pollution Tolerant
04

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o
o
o
(O
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o
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o
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UJ
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                 223     €    2


                   KINDS OF ORGANISMS
           MERRIMACK RIVER TRIBUTARIES


     NUMBERS AND KINDS OF BENTHIC ORGANISMS-1964
                                             FIGURE  3

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               TOLERANT
    SENSITIVE
                                                                                     107      106
                                                                                  RIVER MILE
                                                                                                                                       101
                                                                                                                                                100
                  TOLERANT
  % SENSITIVE
\
             90       69
              RIVER MILE


DISTRIBUTION OF BENTHIC ORGANISMS
        IN MERRIMACK RIVER

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                            TOLERAN
               %  INTERMEDIATE



          X  SENSITIVE
                  82
  74       fa

RIVEN MILE
                                                                                                                                                        e's
                           % TOLERANT
          X SENSITIVE

                    100ft
I
\
                                                                                   RIVER MILE



                                                                      DISTRIBUTION OF BENTHIC ORGANISMS
                                                                              IN MERRIMACK RIVER

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                          TOLERANT
              %  INTERMEDIATE
          SENSITIVE
                 100
                49
                                                   45
                                                                    43
                                                                             42
                                                                                     41       40
                                                                                    RIVER MILE
                                                                                                       39
                                                                                                                38
                                                                                                                        37
                                                                                                                                 36
                                                                                                                                          35
                                                                                                                                                   34
                                                                                                                                                           33
                        % TOLERANT
              %  INTERMEDIATE
         SENSITIVE
|
                                                                                    RIVER MILE
                                                                     DISTRIBUTION OF BENTHIC ORGANISMS
                                                                              IN MERRIMACK RIVER

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              X TOLERANT
X SENSITIVE  _
        •00^
        16       IS       14      13      12       H
                                                           DISTRIBUTION OF BENTHIC ORGANISMS
                                                                  IN MERRIMACK RIVER

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be polluted not only in the general vicinity of the confluence with the



Merrimack River but also for many miles upstream.  Ihinicipal sewage and




wastes from pulping and tanning operations were discharged to the Pemi-



gewasset River.  Raw sewage from Franklin, New Hampshire, was discharged



to the Winnipesaukee River.  The Contoocook River received raw sewage,



paperboard and tannery wastes.  The Piscataquog River was mostly raw



sewage from Manchester, New Hampshire, at the time of sampling.  The



Nashua River received the wastes from paper manufacturers and from raw



and treated sewages.  Municipal wastes from the City of Lowell, Massachu-



setts, were discharged to the Concord River.  Industrial and municipal



wastes were discharged to the Splcket and Shawsheen Rivers.



          A relatively clean stream bed was found in the Whitman River



just upstream of the Route 2A bridge, west of Fitchburg, Massachusetts.



The Whitman River is a tributary to the North Nashua River.



          Samples of bottom sediments taken from the Whitman River re-



vealed a well-rounded population, with nineteen different kinds of



bottom organisms.  Organisms sensitive in their tolerance of pollution



included caddisflies, beetles, mayflies and stoneflies.  Six kinds of



organisms intermediate in their tolerance of pollution were found.  Kinds



of benthic fauna considered intermediate in their tolerance of pollution



are those commonly occurring in naturally enriched organic substrata.



These included beetles, mothflies, midgeflies and clams.  Pollution



tolerant sludgeworms were also found.  These data are presented in



Table 2.






                                  - 5 -

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PEMIGEWASSET AND WINNIPESAUKEE RIVERS



          The Merrimack River is formed by the confluence of the Pemi-



gewasset River, draining the northern mountainous region of New Hamp-



shire and the Winnipesaukee River which drains a large lake system in



the central portion of the state.



          A biological sampling site was established in the Pemigewasset



River O.A.6 miles upstream of its confluence with the Winnipesaukee River.



Raw and partially treated sewage was discharged to the stream by most of



the towns bordering the banks of the Pemigewasset.  These wastes suppor-



ted a lush growth of algae found covering the rocks and rubble in the



stream bed.



          In an unpolluted stream, a rocky stream bed such as this one




with its coating of algae and organic debris, potentially provides abun-



dant cover and nourishment to a large and varied population of benthic



fauna.  However, the actual numbers and types of fauna found here consis-



ted of only 254 individuals per square meter with just nine kinds of



bottom life, mostly herbivorous midge fly larvae.  In comparison to a



relatively unpolluted stream, such as the Whitman River, a tributary to



the North Nashua River in Massachusetts, a total of 3,047 individuals



per square meter and nineteen different kinds of benthic fauna were



found in the bottom sediments (Table 2).



          Especially noteworthy in these sediments from the Pemigewasset



River was the total absence of pollution sensitive insect predator



species, such as the mayflies.



          The meager diversity and paucity of species found here indicates



                                  - 6 -

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that the benthic community was affected by recent upstream organic




pollution.




          A biological sampling site was located in the Winnipesaukee




River, 0.19 miles upstream of its confluence with the Pemigewasset




River.  At this location the water was grey-green, very turbid and




sluggish.  The stream bed was quite rocky.  The bottom sediments con-




tained brown fibrous matter in abundance and sine lied like decomposing




sewage sludge.  Raw sewage discharged at Franklin produced septic condi-




tions in the stream bed and overlying waters.  Gases of anaerobic decomp-




osition bubbled up from the stream bed during dredging of the bottom




sediments.  Insect predator species, such as stoneflies,  which cannot




tolerate poisonous gases resulting from the breakdown of  sewage^2',  were




not found.  Mayflies'3', stoneflies, caddisflies and certain bettles




cannot withstand the low oxygen levels that occur here.  Other more  toler-




ant species, including the snails, leeches and certain midgefly larvae,




were found in large numbers.   A total of 2,033 individuals and seven




kinds of bottom fauna, mostly leeches, were found per square meter of




stream bed.  This large number of a few tolerant species  of bottom fauna,




gases of anaerobic decomposition rising from the bottom sediments, and




the abundance of raw sewage discharged to the stream from Franklin,  New




Hampshire, indicate that these headwaters were grossly polluted.
                                   -  7  -

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REACH I, FRANKLIN TO BOSCAWEN,  (115.70 to  114.04)




          At a biological sampling site, located 0.53 miles downstream



of the confluence, the stream bed was rocky and contained some sludge



in which there were many fine,  grey fibers.  These fibers blanketed the



benthic community and contributed to the reduction of the midgefly and



snail populations.  Respiratory body surfaces and gill structures may



have been clogged by these  fibers, resulting in suffocation.  A total



of 1,46? individuals and eight  different kinds of bottom fauna were found



per square meter of stream  bed. Most of these were leeches, with a total



of 1,120 individuals and four kinds per square meter.  This large leech



population,  tolerant of the pollution of the river and the septic condi-



tions, preyed upon the snail population and further depleted its number.



Any of the kinds of benthic fauna such as  the scuds, sowbugs, scavenger



beetles and  certain herbivorous midgeflies found upstream which may have



been  carried downstream to  this site were  either suffocated or unable to



withstand the septic conditions.  Further  evidence of gross pollution of



this  area was the huge numbers  of rotifers found clinging to the body



surfaces of  the midgefly larvae and leeches.  These rotifers (Conochiloides



sp.)  feed on the bacteria and microcrustacea in waters where active bac-



terial decomposition of organic sludge is  occurring.



          The stream was rapid, shallow and passed over a stream bed



primarily composed of  sand  with some rock  1.66 miles downstream of the



confluence.   This  same stream bed  under unpolluted  conditions would be



 suitable  for the development of many different kinds  of bottom  fauna,






                                   - 8 -

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especially certain mayflies, caddisflies and waterpennies.  However, only




216 individuals and three kinds of benthie fauna were found per square




meter of this stream bed.  Only certain midgeflies,  a few leeches and




sludgeworms could tolerate the grossly polluted environment.




          The Merrimack River from the confluence of the Pemigewasset and




Winnipesaukee Rivers to the end of this reach was grossly polluted and




represented a zone of active decomposition.








REACH 2, BOSCAWEN TO PENACOOK, (113.53 to 102.84)




          Dense growths of aquatic plants (Potomogeton sp.) covered the




stream bed 2.17 miles downstream of the Winnipesaukee and Pemigewasset




Rivers.  In relatively unpolluted streams, prolific  numbers of herbi-




vores such as certain midgeflies and mayflies may be found feeding on




the tissues of these plants.  Innumerable snails browse on the debris




near the roots, and predatory carnivores such as dragonflies and leeches




search for sludgeworms and insects burrowing into the substrate for food




or shelter.




          However,  such a community of bottom life did not exist at this




site.  The assemblage of bottom life found was impoverished both in kind




and number.  Only 615 individuals,  mostly snails and sowbugs,  and five




kinds of fauna were found per square meter of stream bed.   Sewage dis-




charges taking place at upstream locations contributed an abundance of




fertilizer, such as nitrogen and phosphorus,  causing a prodigeous growth




of aquatic plants.   Neither midgefly larvae  nor pollution sensitive in-




sect species such as mayflies were found.  The sparse population and



                                  - 9 -

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paucity of species further characterized this area as one of moderate




pollution.




          Pollution  sensitive caddisflies were found in the Merrimack




River 3.10 and 4.52 miles downstream of the confluence.  Herbivorous




midgeflies were also  found at these locations.  Snails and snail-leeches




were plentiful.  Death  and decay  of aquatic plants at upstream areas




apparently recycled  additional  fertilizer to this site, supplementing




that not used by the upstream plants and causing another abundant plant




growth  (Potomogeton  sp»).  The  Merrimack River showed signs of recovery




at these two locations.




          At 5.10 miles upstream  of the Sewalls Falls Dam, there were a




few clams  (Pisidium  sp.),  leeches, sludgeworms and many snails.  Midgefly




larvae  and the pollution sensitive caddisflies were not found.  Although




aquatic plants grew  in  abundance, providing food, cover and concealment




for the bottom life, only 970  individuals and six different kinds of




benthic fauna were found.  The  few kinds and numbers of bottom life and




the prolific aquatic plant growth indicated that moderate pollution still




existed in the stream.




           The  stream in this entire  section may be characterized as one




of moderate pollution but showing signs of recovery.  Most of this




river bed was  covered with a dense plant growth nourished and sustained




by the fertilizer  from  sewage  discharged upstream.









REACH 3,  PENACOOK  TO CONCORD,  (100.71  to 86.80)




          As a result of the raw  discharge of the Brezner Tanning




Corporation, Boscawen,  New Hampshire,  massive organic pollution




                                   -  10 -

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occurred in the Contoocook River one-half mile upstream of the confluence.




The stream was clogged with rafts of decomposing sludges four to six



inches in dimension, floating downstream to the Merrimack River.  When the



stream bed was disturbed, large volumes of decomposition gases and grey



fibrous matter rose to the surface.  The only benthic fauna found in the



bottom sediments were leeches.  Even these numbered only ninety-four



individuals per square meter of stream bed.  Other kinds of fauna which



may have been carried downstream from areas in the Contoocook faced suffo-



cation by clogging of respiratory surfaces with the fibrous matter dis-



charged from the tannery, as well as death by the septic environment.



          The Merrimack River was still in a zone of moderate pollution



5.41 miles downstream of the Contoocook River, although most of the



organic sludges originating in the Contoocook had settled out behind



Sewalls Falls Dam.  Only four kinds of benthic fauna and 127 individuals,



mostly sludgeworms, were found per square meter of stream bed.  There were



also a few leeches, snails and midgefly larvae in these bottom sediments.



Pollution sensitive fauna were not found.



          Farther downstream of Sewalls Falls Dam,  the river recovered



somewhat from the organic pollution very evident in upstream locations.



Nine kinds of bottom fauna and 173 individuals were found per square



meter of stream bed.  Sediments removed from this area contained a few



midgefly larvae, scuds, snails, leeches and sludgeworms.   Even a few



pollution sensitive caddisflies and riffle beetles  were found in these



sediments.




          Five hundred feet downstream of the Poute 4 bridge in Concord,



                                  - 11 -

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New Hampshire, floating sludge masses with a septic sewage odor occurred.




Bottom sediments dredged here were foul-smelling and were chiefly sewage




sludges discharged from Concord.  Large numbers of benthie fauna tolerant




of the organic pollution were found in the sludge.  Eight kinds of bottom




life and 1,356 individuals, mostly sludgeworms, were found per square




meter of stream bed.  Benthic fauna found included clams, mussels,




leeches, midgefly larvae and snails.  Although the stream bed was gravel




and potentially suitable for the case-making types of caddisflies found




upstream of Concord, these nymphs could not have tolerated the septic




environment.




          In  summary, this reach may best be described as one undergoing




active decomposition of the organic pollutants discharged to the Merrimack




River by the  Contoocook River, moderate recovery shortly downstream of




Sewalls Falls Dam, followed by another zone of gross organic pollution




caused by the municipal wastes of Concord, New Hampshire.









REACH 4, CONCORD TO HOOKSETT, (86.80 to 81.05)




          Dredging of the stream bed 0.20 and 0.50 miles, respectively,




downstream  of Garvins Falls Dam produced only an impoverished assemblage




of bottom fauna, consisting of a few sludgeworms and midgefly larvae.




At these locations, the river was still In a zone of moderate pollution




even though most of the sewage sludges discharged at Concord had settled




behind the  dam.




          In  the Soucook River, 0.04 mile upstream of its confluence with




the Merrimack River, and in the Merrimack River, one mile downstream of




                                  - 12 -

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this confluence, only a few kinds and numbers of bottom fauna, mostly




sludgeworms, were found in the bottom sediments.  These sediments also




contained snails, leeches and craneflies.




          In a ponded section of the Merrimack River,  2.63 miles upstream




of the Hooksett Dam, the sediments were composed mostly of silt and




organic sludge.  The small number of predatory leeches and the abundant




food supply favored the development of a large number  of omnivorous




snails—348 per square meter were found.




          Bottom sediments in the Suncook River, 0.2 miles upstream of




its confluence with the Merrimack River, contained large numbers of




snails and leeches, as well as a few clams and sludgeworms.  These kinds




of fauna flourish in quiescent,  ponded areas enriched  with dissolved




nutrients, especially where rooted aquatic plants are  available to




supply food, cover and concealment.  There was an extensive growth of




pondweeds (Potomogeton sp.) throughout this sampling area.  This loca-




tion was in the backwater of the Merrimack River.  Sewages supplied abun-




dant nutrients and fertilizing elements to nourish both the flora and the




fauna.




          Bottom sediments dredged from the stream bed 0.19 miles upstream




of the Hooksett Dam were black and had a septic odor,  and consisted




chiefly of sand, silt and organic sludges.  A few midgefly larvae and




dragonfly nymphs were found in these sediments.  Other insect species,




such as mayflies and certain caddisflies, could not tolerate the septic




condition of the sediments and overlying waters.  Although predatory




leeches were found, they were few in number.  Municipal discharges up-




                                  - 13 -

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stream contributed organic enrichment favoring development of the many



snails and mussels found.  A total of 352 snails and 120 mussels were



found per square meter of stream bed.



          Impoverished assemblages of benthic fauna in some areas, large



numbers of a few species in other areas, and prolific growths of pond-



weeds in backwater sections indicate that gross to moderate pollution



existed in this section of the Merrimack River.








REACH 5, HOOKSETT TO MANCHESTER, (81.05 to 73.14)



          This reach of the Merrimack River extends from the Hooksett



Dam to the Amoskeag Dam in Manchester, New Hampshire.



          Bottom sediments one-half mile downstream of the Hooksett Dam



had a foul septic sewage odor.  Anaerobic decomposition of the sewage,



blood and paunch manure discharged to this area rendered the stream bed



ineffectual as a habitat for most benthic fauna except for a few snails



and leeches.



          Bottom fauna in sediments dredged 3-45 miles downstream of



the Hooksett  Dam indicated that some improvement of the river had



taken place.  These fauna included many midgefly larvae, snails, leeches



and even a few pollution sensitive caddisfly larvae.  Eleven kinds of



bottom fauna  and 1,231 individuals were found per square meter of stream



bed.



          Conspicuous and favorable improvement of the benthic environ-



ment was found 4.26 miles downstream of the Hooksett Dam.  Large numbers



of individuals (1,845 per square meter) and sixteen kinds of caddisfly




                                  - 14 -

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larvae, midgefly larvae, cla'.is, snails, scuds, leeches and sludgeworms



were found.  The great diversity of benthic fauna found was not equalled



or surpassed in any other location sampled in the Merrimack River.



          Benthic sediments were black and had a septic odor near the



end of this reach, located 1.03 miles upstream of the Amoskeag Dam.  Muni-



cipal waste dumped into the river from northern Manchester contributed to




the septic environment.  Although certain species of caddisfly larvae can



tolerate low dissolved oxygen concentrations   , the septic environment



would kill any of these larvae, such as those found upstream, which may



have been dispersed to this area.  Other kinds of benthic fauna, such as



leeches and snails, apparently tolerated this type of environment as they



were found in large numbers.  Clams, midgefly larvae and mussels were



also found, since organic food was abundant in these sediments.



          Although there was some recovery evident in the central portion



of this reach, both the first and last portions were grossly polluted.








REACH 6, MANCHESTER TO NASHUA, (73.14 to 55.75)



          Massive organic pollution occurred in the first two miles of



the Merrimack River downstream of the Amoskeag Dam.  Sewage and industrial



wastes from the city of Manchester were discharged to this section.  The



flow of the Piscataquog River consisted chiefly of the sewage from Man-



chester.




          When the sediments were dredged from the stream bed 5.09 miles



downstream of the Amoskeag Dam, few benthic fauna were found.  There



were only 453 individuals "per square meter of stream bed.  Although ten



                                  - 15 -

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different kinds of bottom life were present, most of these were certain



pollution tolerant midgefly larvae and leeches.  A few mussels, snails



and sludgeworma were also found.  These  few individual representatives



of the several different kinds of benthic  fauna  found indicate that



population depletion may have occurred not only  as a result of the septic



environment but also by suffocation brought about through settling of



organic wastes discharged upstream.   Scouring  of the river bed occurred



downstream of the Amoskeag  Dam during peaking  power operations at the



dam.  Scouring in this area led  to mixing  and  resuspension of sewage and



slaughterhouse and  other industrial wastes, as well as settled organic



sludges.  The prolific growth of pondweeds (Potomogeton  sp.) observed



suggested the highly organic  nature'^) Of  the  soil, as well as attesting



to the  excessive  fertilization of this stretch of the river.  In addi-



tion, the body surfaces of  the midgefly  larvae and leeches taken from



the  sediments were  covered  with  rotifers.   These rotifers (Conochiloides



sp.) were often  found attached to benthic  fauna  found in areas of the



Merrimack River  known to receive gross organic pollution with sewage.



          Resuspension of sediments occurred in  the vicinity of Goffs



 Falls,,New Hampshire.   Deposition and decomposition of these sediments



 caused  the  sparse population of  benthic  fauna  found at river mile 65.11.



 Only two kinds  of bottom fauna,  certain  pollution tolerant midgefly



larvae  and  sludgeworms, totaling 516  individuals, were found per square



meter of stream bed.



          Organic pollutants  discharged  into the Souhegan and Merrimack



 Rivers  provide an ample food supply.  However, 3.0? miles downstream of



                                  - 16 -

-------
 their confluence, only five kinds of benthic fauna and 269 individuals




 were found per square meter of stream bed.  This fauna consisted of




 sludgeworms and a few midgefly larvae and leeches.  The lethal action of




 the New England Pole and Wood Treating Corporations' discharge of phenols




 (2.32 miles upstream) caused the small size of the population.




          The lethal action of phenol on fish has received considerable




 study.  Wuhrmann and Woker, in a review^°' of the literature on the toxi-




 city of phenol to fish, quote a number of limiting concentrations for




 various species ranging from 0.5 ppm to 20 ppm.   The mussel fauna may




 very well have been eradicated from this section of the river because of




 their dependence in their life cycle on fish hosts.  Concentrations of




 phenols in the river muds at river mile 61.18 were found to equal 8,000




 ppm.  Since phenols are also known to cause an intense irritant action




 on mucous membranes, mussels,  clams and snails would suffer starvation




 and respiratory failure.




          Benthic fauna found  in sediments farther downstream were




 chiefly sludgeworms, with 7,092 worms found per  square meter of stream




 bed.  Except for a few midgefly larvae and these sludgeworms, no other




 form of benthic fauna was found in these sediments.  Other forms of benthic




 fauna such as clams, mussels and snails may have been eradicated by pheno-




 lic substances or smothered by the large quantities of grease and oil




 found in the bottom sediments.




          Bottom sediments at  river  miles 58.10  and 57.91  also contained




only a few midgefly larvae and sludgeworms.




          Some improvement in  the stream bed took place 2.60 miles up-




                                  -  17 -

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stream of the confluence of the Nashua and Merrimack Rivers.  Four
different kinds of benthic fauna, including mussels, snails, sludge-
worms and even caddisfly larvae, were found.
          Reach 6 suffered gross organic and chemical pollution.  There
was some improvement at the end of the reach, however.

 REACH 7, NASHUA TO NEW HAMPSHIRE-MASSACHUSETTS STATE LINE, (54.80 to 49.82)
          Dredgings from the  stream bed in the Nashua River were black
and had  a septic sewage odor. Discharges from upstream paper manufac-
turing operations and municipal sewage from Nashua, New Hampshire, contri-
buted to the condition.  Only two kinds of benthic fauna were found in
these sediments—midgefly larvae and sludgeworms—and just sixty-four
individuals per square meter  of stream bed.  Other benthic fauna such as
snails and  clams found upstream in the Nashua were not found here.  These
fauna either could not tolerate the septic environment or  were  smothered
by the settling solids.
          Upstream in the Nashua River Canal, the bottom sediments con-
sisted chiefly of paper manufacturing sludges and contained a huge popula-
tion of  midgefly larvae, 6,856 larvae per square meter, and sludgeworms,
1,294 worms per square meter. The abundance of food and lack of predatory
 fauna favored development of  pollution tolerant life.
          No benthic fauna were found in sediments from the Merrimack
River 0.55  miles downstream of the confluence with the Nashua.  During
dredging of the stream bed, nauseous gases of anaerobic decomposition
bubbled  to  the surface.  This portion of the Merrimack River WAS in a
                                  - 18 -

-------
 state  of active decomposition.  Benthic fauna dispersed to this area




 from upstream locations would face death by exposure to this septic en-




 vironment or be smothered by fibrous matter found in abundance in these




 sediments.




          Few benthie fauna except certain midgefly larvae and sludge-




 worms were found in bottom sediments dredged at three additional down-




 stream locations at river miles 52.81, 52.72 and 52.53.  In addition to




 the limiting or lethal septic environment in these areas,  survival of




 these few benthic fauna was further endangered by oil and grease,  especi-




 ally noticeable in the sediments taken at river mile 52.72.  Oil and




 grease coat the respiratory surfaces of bottom fauna, causing death by




 suffocation.  Just as in other upstream locations receiving gross  organic




 pollution, sediments dredged at river mile 52.53 contained certain midge-




 fly larvae completely covered with rotifers.   Whatever oxygen resource




 was still available to the larvae in this septic environment  became even




 less available because of the decreased respiratory  surface area used




 as points of attachment by these rotifers.




          Throughout most of this reach,  the Merrimack River  was in a




 state of active decomposition.   With the exception of a few midgefly




 larvae and sludgeworms, no other kinds of benthic fauna were  found in




 sediments from the river bed.









REACH 8,  NEW HAMPSHIRE-MASSACHUSETTS STATE LINE TO LOWELL,  (49.82  to 40.60)




          Septic conditions were especially noticeable during dredging




 ol the bottom at river miles 48.37,  44.69 and  43.46,  just  downstream




 of the New Hampshire-Massachusetts state line.   Nauseous gases




                                  -  19 -

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bubbled out of the river bed and the sediments were black and odorous.



Grease and oil were found in sediments at river mile 46.82.  Fine grey



fibers were found in dredgings from the river bottom at river miles 43.46



and 42.52, downstream of two wool-scouring plants.



          Only one to four kinds of benthic fauna were found in this



reach of the river.  Sludgeworms ranged from 24-2,104 per square meter,



midgefly larvae 0-8 per square meter, mussels 0-16 per square meter and



snails 0-16 per square meter.  No other benthic fauna were found in



sediments dredged from the river bed.  Septic conditions suppressed or



killed most benthic fauna.  Others faced death by suffocation brought



about by clogging of respiratory surfaces with solids or by coating of



these surfaces with grease and oil.



          This reach showed very little improvement, continuing through-



out most of its length in a zone of active decomposition.







REACH 9, LOWELL TO LAWRENCE, (40.60 to 28.99)



          Reach 9 extends from the Pawtucketville Dam at Lowell, Massa-



chusetts, to the Essex Dam at Lawrence.  Except for a rapids area extend-



ing about three miles downstream of the Pawtucketville Dam, the remaining



portion of this reach is in quiet water as a result of the backwater of



the Essex Dam.  The reach was found to be grossly polluted by the dis-



charge of organic wastes.



          Decomposition of bottom sediments was especially remarkable at



two locations, river miles 36.36 and 36.30, downstream of the confluence



of. the Concord and Merrimack Rivers.  Gat-lifted fecal matter and putrid



                                  - 20 -

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 sludge* floated about the water surface.  During dredging of the  stream




 bed, decomposition gases bubbled to the surface.




          An extensive and varied assemblage of benthic fauna such as




 certain burrowing mayflies, caddisflies, mussels and clams would under




 unpolluted conditions occupy a stream bed of this type.  However, only




 sludgeworms (8-299 per square meter), midgefly larvae (0-347 per square




meter) and, in one location, leeches (42 per square meter) were found.




 These few kinds and numbers were the only benthic fauna surviving in




 the polluted sediments.  The septic sludge and overlying water markedly




 reduced available oxygen.  A further hazard to survival of the midgefly




 larvae were the numerous rotifers found attached to their body surfaces,




 thereby reducing the available respiratory surface area.  These rotifers




were especially noticeable on the fauna found in the sediments at  river




miles 36.36, 35.11 and 31.92.




          As in the reach upstream,  this reach was in a zone of active




decomposition throughout most of its length.   Only a few pollution




tolerant leeches,  midgefly larvae  and sludgeworms  were found.









REACH 10,  LAWRENCE TO HAVERHILL,  (28.99  to 15.70)




          The reach is broken down into  three sections.   Section 1  is




that portion of the river between  the cities  of Lawrence and Haverhill.




Section 2  is that  portion through  Haverhill and extending downstream




to Buoy 61.  Section 3 extends from  Buoy 61 to the  Groveland Bridge.




          Gases of anaerobic  decomposition bubbled  up from the  stream bed.
                                   -  21  -

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Gas-lifted fecal matter and islands of decomposing organic filth floated



throughout the sampling area.  Just prior to dredging, samples of water



taken within a foot overlying the stream bed were found to be acid



(pH 6.3-6.9) with concentrations of dissolved oxygen ranging from 1.2 to



4.1 mg/1 (T = 20°C).  Stream bed sediments were primarily organic and



had a strong sewage odor.



          The only benthic fauna found in section 1 were midgefly larvae



and sludgeworms, except for a  few leeches found at river mile 28.50.  No



benthic fauna were found at river mile 25.35.  In general, snails are



uncommon in streams whose surface waters are more acid than pH 6.2 and



require rather high concentrations of dissolved oxygen'''.  The acid



waters and septic conditions prevailing in this section would limit, if



not prevent, the development of snail populations.  Also, leeches which



do not appear to be able to tolerate  gases of anaerobic decomposition



at low oxygen tensions''' were not found, nor could they survive in



this section of the river where decomposition gases as well as low con-



centrations of dissolved oxygen occurred.  An abundance of dissolved



oxygen also appears to be an environmental necessity'?) to scuds.  Scuds



were not found in this section of the river.  Most of the midgefly lar-



vae and all of the  sludgeworms contained red blood pigments which enabled



them to survive the low dissolved oxygen levels common to this section.



           The  second  section resembled the first in that decomposition



gases rapidly  rose  to the river surface during dredging.  Gas-lifted



islands of fecal matter and decomposing sludge up to four inches in



dimension  were  abundant.  Sludge  formed accumulations up to six inches



                                  -  22 -

-------
deep along the river banks at river mile 17.30 downstream of Haverhill.



Dissolved oxygen concentrations in this section ranged from 1 to 2.5



mg/1 (T = 20°C) in water immediately above the stream bed.  The pH ranged



from 6.4 to 6.?.  Bottom sediments in this section were mostly organic,



black and had the stench of septic sewage.



          There were a few leeches, snails and even a few marine clams



in the sediments dredged at river mile 19.62.  Midge fly larvae and



sludgeworms were the predominant benthic fauna found in this section.



As had been observed in other areas of the Kerrimack which were grossly



polluted with organic matter, certain rotifers were attached in great



numbers to the body surfaces, especially the gills, of the midgefly



larvae, thereby reducing the respiratory surface area and making it



even more difficult for these larvae to survive.



          This section, subjected to tidal action,  is a mixohaline region.



Very few species can survive in this region; therefore,  one would not



expect to find either very many or much diversity.   However, several kinds



of marine fauna can adapt to salinities less than those found in the sea,



such as certain sowbugs (Cvathura carinata) and scuds (Gammarus sp.)



Neither of these marine forms were found here, but  they did appear at



the next downstream stations.  Although certain fresh-water animals will



tolerate variations in salinity, such as sludgeworms and certain midgefly



larvae, most find tidal waters uninhabitable because the organisms do not



contain structures or mechanisms for maintaining a  proper salt balance.



It is important to note that at river miles 19.35 and 17.75, no benthic



fauna of either fresh or marine origin were found.   Without doubt, gross



                                  - 23 -

-------
organic pollution in this section was responsible for the lack of either




fresh or marine benthic fauna found here.




          In section 3, bottom sediments were composed mostly of sand




and rock with some organic sludge.  Septic conditions existed at river




mile 16.51 where the dissolved oxygen level a foot over the stream bed




was 1.9 mg/1 (T = 21°C).  Some gas bubbled to the surface in this same




area during dredging.  Leeches,  clams, midgefly  larvae, scuds and sludge-




worms were found.  Rotifers, abundant on the body surfaces of the midge-




fly larvae and leeches, were nourished by the bacteria and microcrustacea




supplied through decomposition of the bottom sediments.  Low oxygen




levels  and septic conditions are known to favor  certain kinds of benthic




fauna,  such as sludgeworms, resulting in great numbers of them.  The




sludgeworm population at  river mile  15.68 was very  large, with 14,972




worms per square meter.   The  stream  bed did  support  greater numbers of




both marine and  fresh water fauna, but these were forms of bottom life



that  could tolerate the gross  organic pollution  in  this section.








REACH 11, HAVERHILL TO ATLANTIC OCEAN,  (15.70 to 0.00)




           This reach is divided into two  sections.   Section 1 extends




 from the Grove land Bridge to the Route  1 Bridge, river mile 2.91.




 Section 2 extends from the Route 1 Bridge  to the ocean.



           Throughout the first section,  the  sediments were composed of




 silt and sand.  Dissolved oxygen concentrations  ranged  from 2.1  to 3.6




 mg/1 in the water one foot above the stream bed. For the first  8.42






                                    - 24 -

-------
miles of this section the temperature one foot above the stream bed was
20°C.  The remaining portion of 4.37 miles reflected tidal excursion up-
stream with temperatures dropping to 17.5°C at river mile 4.10.  Through-
out this section, decomposition gases bubbled to the surface during
dredging.
          Benthic fauna of freshwater origin in the sediments from Sec-
tion 1 consisted of midgefly larvae and sludgeworms.  Marine fauna in
these sediments included scuds, sowbugs and marine worms.  Downstream of
river mile 7.80, freshwater populations of midgefly larvae and sludge-
worms markedly declined in number.  Neither group existed at the end of
the section.
          Several factors led to the demise of the freshwater fauna
and the absence of saltwater fauna.  Although organic matter of sewage
origin was especially noticeable in the silty bottom sediments upstream
of river mile 7.80, bottom sediments at downstream locations were com-
posed of relatively clean sand with enough organic matter to support
anaerobic bacterial decomposition.  Availability of food for life sup-
port* ' apparently was not a limiting factor in this area.  The unstable
stream bed brought about by tidal action was the more probable cause for
the decline in fresh and saltwater benthic fauna.  Only a small number
of marine scuds and sowbugs penetrated the polluted waters in this
section.
          Section 2 of this reach encompasses the estuarine portion of
the Merrimack River.  The partially treated sewage from the towns of
Salisbury and Newburyport, as well as^ wastes carried to this area by the
                                  - 25 -

-------
Merrimack River, nourish an abundant benthic fauna and flora.  At river



mile 1.73> about one-half mile downstream of the Newburyport sewage out-



fall, marine worms numbered 6,399 per square meter.  Even freshwater



sludgeworms in sediments dredged at this location numbered 2,459 per



square meter.  Large numbers of clams, mussels, scuds and sowbugs were



also found in the sediments.  Sea lettuce flourished in the estuary,



especially just west of Woodbridge Island and Black Rock Creek.  A sum-



mary of total tolerant and total organisms found in the estuary is shown



in Figure  5.-
                                   -26 -

-------
LEGEND
5627
7062,
         Total Toltrant Organisms/
                                               Black Rock
                                                 Creek
    Totol Organisms/
 *
Inter tidal Area
                                                                            ATLANTIC
                                                                               OCEAN
      Newburyport
                                                                              The Basin
                      BENTHIC ORGANISMS IN MERRIMACK RIVER ESTUARY- 1964

-------
                    BOTTOM ORGANISMS OF SOUHEGAN RIVER








          In late May and early June, 1965, a biological survey was



carried out on the lower Souhegan River, a tributary which discharges



into the Merrimack River 12.5 miles upstream of the New Hampshire-



Massachusetts state line.  The section studied extended from just up-



stream of Wilton, New Hampshire, to the mouth of the Souhegan.  A list



of the sampling stations and reference points is presented in Table 4-



          Three locations were sampled upstream of Wilton—two in the



Souhegan River and one in Stony Brook.  The only known source of pollu-



tion occurs at Greenville, New Hampshire,  about 8.4 miles upstream of



Wilton, where raw sewage from approximately 500 persons is discharged.



In each of these three locations, pollution sensitive organisms were



found to be predominant both in total numbers and in their diversity of



species (Table 5 and Figure 6).  The river at mile 21.46 was cool



(T = 15 C) and shallow, with a rocky bed and fast current.  The water



was soft (Hardness = 12 mg/1 as CaCO^), low in alkalinity (5 mg/1 as



CaCO-j) and well oxygenated (Dissolved Oxygen = 10.8 mg/1).



          The Souhegan River between Wilton and Milford, New Hampshire,



deteriorated considerably, with pollution tolerant leeches and sludge-



worms making up most of the benthic fauna found.  Textile operations



and raw sewage from Wilton accounted for the polluted condition in the



river.  The dissolved oxygen dropped to 7.1 mg/1 but there was little



change in hardness, alkalinity and temperature from upstream.  Through-



out most of this reach, the stream bed was rocky and the current moderate,




                                  - 27 -

-------
          Moderately polluted conditions continued to exist in the stream



for several miles downstream of Milford, which discharges the raw sewage



of approximately 3,000 persons.  Gases from decomposition of sludge de-



posits were noted at river mile 8.42.  The stream meandered throughout



this section and had a moderate current and shallow depth.  The stream



bed was mostly sandy with some gravel and loam.  Dissolved oxygen contin-



ued high.



          By the time the Souhegan River reached the Amherst-Merrimack



town line, the river showed signs of recovery from a biological stand-



point.  Bottom organisms generally found in moderately polluted environ-



ments, such as certain midgeflies and snails, assumed dominance both in



species diversity and in percentage of total organisms.  There was a



marked decline in the percentage of pollution tolerant individuals com-



pared to the section Just downstream of Milford (Figure 6).  The stream



bed was sandy with some sandy loam.  The shallow depth and moderate cur-



rent continued.  Dissolved oxygen increased from 7.3 mg/L at river mile



6.51 to 9.0 mg/1 at river mile 3.12 at a temperature of 15°C.



          Sampling of the river in a riffle area just upstream of Wild-



cat Falls, river mile 1.15, showed the continued dominance of benthic



fauna generally found in moderately polluted streams.  However, there



was an increase in the proportion of tolerant forms.  Similar conditions



were found in the sample taken just downstream of the Everett Turnpike



and upstream of the waste discharges of Merrimack, New Hampshire.



          Bottom organisms that were sensitive to pollution were found



at all sampling sites except at river mile 14*49 at Milford.  Where



                                  - 28 -

-------
                         WILTON
                      MILFORD
        % TOLERANT
  % INTERMEDIATE/^
% SENSITIVE/7
    100 -
-------
these organisms were found, those occurring most frequently were the



caddisflies, mayflies and riffle beetles.



          The greatest numbers of tolerant organisms were found between



river miles 18.1? and 8.42, forty-seven per cent of the length of stream



studied.  Sludge worms were the tolerant kind most frequently found.



          From a biological standpoint, the river was moderately polluted



from Wilton, New Hampshire, to the confluence with the Merrimack River,



a distance of twenty miles,
                                 - 29 -

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                    PRODUCTIVITY OF THE MERRIMACK RIVER

          A productivity study of short duration of the Merrimack River
between Manchester, New Hampshire and Lowell, Massachusetts, was initia-
ted in August 1965.  Three sampling stations were selected at river
miles 65.11, 48.76 and 43.47-  The data were plotted downstream of the
Queen City Bridge in Manchester, New Hampshire, (river mile 71.G?) to
indicate the productivity of the stream after passing through the major
cities of Manchester and Nashua in New Hampshire.
          Algae are reported^) '*™,.to D6 adversely affected in culture..
media when the concentration of inorganic nitrogen falls below 0.2 mg/L
and that of phosphorus below 0.05 mg/1.  Sawyer reported in the Madison
Lakes survey'^ *' that nuisance algae conditions were expected when in-
organic phosphorus was found in excess of 0.01 mg/1 and an inorganic
nitrogen level of 0.30 mg/1*  Recently, Maloney'^' reported that algal
growth was exponential in concentrations of detergent phosphorus above
0.1 mg/1 as phosphorus.  Reference to Table 6 indicates that the nitrogen
and phosphorus levels found in the Merrimack River were obviously not
limiting to potentially abundant growths of phytoplankton at any of
the stations.
          As indicated in Figure 7, photosynthetic oxygen production,
concentration of chlorophyll a, and the total number of phytoplankton
increased downstream of the Queen City Bridge.   The inflow of nutrient
phosphorus and nitrogen was potentially capable of supporting an abundant

                                  - 30 -

-------
  •8
  2
  -g
•s E
2? *>
C o
It
  ro
   E
  o
  zi
  o
100

90

80

 6
 I
 4

 2

 0

30

20

10
          800
          600

          400
          200
                      PRODUCTIVITY OF THE
                 MERRIMACK RIVER-AUGUST 1965
                   (Data taken from I- foot depth)
                          FLOW
                          OXYGE
                          CHLOROPHYLL a
                ALGAE
                     K>
                     J5.
25-
            Miles Downstream of Queen City Bridge, Manchester, N.H.
                                                    FIGURE 7

-------
growth of phytoplankton in any of the three reaches.  The backwater


effect of the dam at Lowell, Massachusetts, caused an environment in


the two downstream stations more favorable to the growth of algae.


Chlorophyll a was measured in accordance with the procedure outlined by

                 ( »0\
Creitz & Richards^ J' and proved to be a less time-consuming method for


the estimation of standing crop than that of the identification and


enumeration of algae.  Photosynthetic oxygen production was measured by


the light and dark bottle technique,


          In this study, the use of several tools—photosynthetic oxygen


production, measurement of chlorophyll a, enumeration of algae—appear


necessary to fully interpret productivity especially where nutrient


levels were sufficient to cause an abundant growth in any of the stations


studied.  These tools adequately reflected a relative increase in produc-


tivity in the Merrimack River downstream of the  Queen City Bridge to the


City of Lowell, Massachusetts.
                                 - 31  -

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                 MICROSCOPIC HJLHKTON IN MERRIMACK RIVER








          The surface water of the Merrimack River at the entrance to



the Essex Canal in Lawrence was monitored periodically for phyto»- and



zooplankton during April through October 1965.  Samples were hand dipped



and then brought to the laboratory where the microscopic plankton were



concentrated by the Sedgewick-Rafter method.  The algae were identified



as to genera and the concentration reported in areal standard units per



ml of the sample (ASU/ml).  The data are shown in Figure 8 and Tables



7 and 8.




          The diatoms gradually increased from a low average of 348



ASU/ml in April to a maximum of 931 ASU/ml in July.  In order of decrea-



sing occurrence, those genera of diatoms found were Melosira, Synedra,



Asterionella, Navicula and Fragilaria.  Except for Asterionella, all of



the other four most abundant diatoms were listed by Palmer^  ' as most



tolerant of pollution*



          The green algae rapidly increased from a low average of twenty



ASU/ml in April to a maximum of 3,285 ASU/ml in July, after which the



average count fell to 1,289 ASU/ml in October.  Again, of the five most



abundant genera found, four of the five genera were included'1'4-' among



the fifty-two most tolerant genera of algae.  These were, in order of



decreasing occurrences  Scenedesmus, Eudorina, Pediastrum and Fandorina.



          The blue-green algae were not found to any significant extent



except in July, when the average count was 1,210 ASU/ml.  Only species



of Coelosphaerium, Anabaena, Oscillatoria and Polycystis were found.



                                  - 32 -

-------
                     STANDING CROP OF PLANKTON - 1965
                       MERRIMACK RIVER AT LAWRENCE
o *°
V 30
S 20
£ 1°
5  0
 £
 to
o
<  6000
o
z
 CO
 Ul
 qc 4000
 O
 o
 0 2000
 Q.

 O*
                                          5987
               728
                                  1736

                                           Wjv:v
                                           13
               APRIL
                        MAY
JUNE
JULY
                                                    Blue-Greens
                                                    Flagellates
                                                    Green Algae
                                                    Diatoms
                                                    1464
                                                    I
                                                            1832
SEPT.
OCT.

-------
Of these four genera, Oscillatoria and Anabaena are most tolerant of,



pollution.  Blue-greens were not found in September and October.



          The flagellated protozoa found most frequently were Chlsmydo-



monas, Dinobryon, Syhura, Mallomonas and Euglena.  Both Euglena and



Chlamydomonas are considered the genera most tolerant of pollution.



          Early summer showed a marked rise of the zooplankton.  Codo-



nella and Vorticella species were the most common ciliates found.  The



genera of rotifers which were found most frequently were Anuraea, Syn-



chaeta, Polyarthra and Triarthra.  Daphnia, Cyclops and Bosnri.na.were



the most common Crustacea found.  Both the rotifers and Crustacea ap-



peared to be more abundant during the summer and autumn than in the



spring period.
                                  -33 -

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                         SUMMARY AND CONCLUSIONS








          The biological conditions, with few exceptions, show that the




Merrimack River is grossly polluted from Franklin, New Hampshire, to its




mouth at Newburyport, Massachusetts.




          Benthic organisms sensitive to pollution were absent from the




samples taken in the lower fifty-seven miles of the Merrimack River.




In only four extremely short portions of the river, consisting of less




than fifteen miles out of the total river mileage of 116, did the river




recover enough from its despoiled condition to permit a small number of




sensitive organisms to exist before additional wastes reduced the quality




of the river.  These four areas were: four miles below the confluence of




the Pemigewasset and Winnipesaukee Rivers) above Concord, New Hampshire,




in the reservoir behind Amoskeag Dam) and just above the Nashua River




confluence.




          Organisms intermediate in their response to pollution were




predominant from Franklin, New Hampshire, to the confluence of the




Contoocook River.  Additional waste discharges between the Contoocook




River and the Suncook River resulted in an increase in the proportion




of pollution tolerant forms.  Between Hooksett and Manchester, New




Hampehire, the majority of bottom organisms again were of the types




intermediate in their resistance to pollution.  From Manchester to




Amesbury, Massachusetts, a distance of sixty-six miles, pollution tol-




erant organisms constituted the entire benthic population or the majority




of the forms found.



                                 - 34 -

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          The i-umber of species found in the Merrimack River was far




below the levels desired in a benthic community.  Pollution sensitive




benthic fauna, such as mayflies, stoneflies and certain beetles, were




not found in the river from Manchester, New Hampshire, to the Atlantic




Ocean.



          A number of tributaries were sampled near their confluences




with the Merrimack River.  Results show that all of the sampled areas




were polluted.  In most cases, wastes were discharged into the lower




part of the tributary and affected the bottom fauna.




          A biological survey was carried out on the lower Souhegan




River, a tributary which discharges into the Merrimack River 12.5 miles




upstream of the New Hampshire-Massachusetts state line.   Between Wilton




and Milford, New Hampshire, the Souhegan deteriorated considerably,




with pollution tolerant leeches and sludgeworms making up most of the




benthic fauna.  This polluted condition of the river continued for




several miles downstream of Milford.  From a biological  standpoint, the




river was moderately polluted from Wilton,  New Hampshire, to its conflu-




ence with the Merrimack River, a distance of twenty miles.




          A productivity study of the Merrimack River was conducted




between Manchester, New Hampshire,  and Lowell,  Massachusetts,  that




reflected a relative increase in productivity as the river flowed down-



stream.




          The surface water of the Merrimack River at the entrance to



the Essex Canal*in Lawrence was monitored periodically for phyto- and






                                   - 35 -

-------
cooplankton from April through October 1965.  Most of th* kinds of



phytoplankton found v«r« tolerant of pollution.
                                    -  36  -

-------
                                REFERENCES








1.       Report on Pollution of the Meirimack River and Certain Tribu-



         taries - Part V - Nashua River, U. S. Department of the Inter-



         ior, Merrimack River Project, Lawrence, Massachusetts, August



         1966.





2.       Gaufin, A. R., Environmental Requirements of Plecoptera,



         Third Seminar, 1962 - Biological Problems in Water Pollution,



         USDHEW,. Cincinnati, Ohio.





3.       Ibid., Leonard, J. W., Environmental Requirements of Ephemop-



         tera.





4.       Ibid., Roback, S. S., Environmental Requirements of Trichop-



         tera.





5.       Macan, T. T., Freshwater Ecology, John Wiley and Sons, Inc.,



         pp. 213 and 255, 1964-





6.       Jones, J. R. E., Fish and River Pollution, Butterworths,



         London, pp. 144-151, 1964.





7.       Pennak, Robert W., Fresh Water Invertebrates of the United



         States, Ronald Press Company, New York, 1953.





8.       Brinkhurst, R. 0., The Biology of the Tubificidea with Special



         Reference to Pollution, Third Seminar 1962 - Biological Prob-



         lems in Water Pollution, USDHEW, Cincinnati, Ohio.




                                 - 37 -

-------
 9.       Chu, S. P., The Influence of the Mineral Composition of the
          Medium on the Growth of Planktonic Algae, Part I.  Methods
          and Culture Media, Journal of Ecology, Vol. 30, No. 2, pp.
          284-325, 1942.
10.       Ibid., Part 11.  The Influence of the Concentration of Inor-
          ganic Nitrogen and Phosphate Phosphorus, Vol. 31, pp. 109-148,
          1943.
11.       Sawyer, C. N., Some New Aspects of Phosphates in Relation to
          Lake Fertilisation, Sewage and Industrial Wastes, Vol. 24,
          No. 6, pp. 768-776, 1952.
12.       Maloney, T. £., Detergent Phosphorus Effect on Algae, Journal
          Water Pollution Control Federation, Vol. 38, No. 1, pp. 38*45,
          January 1966.
13.       Creiti, 0. I., and Richards, F. A., The Estimation and Charac-
          terization of Plankton Populations by.PifwntJ Analysis.  III.
       .   A Note on the Use of Millipore Membrane Filters in the Estima-
          tion of Plankton Pigments, Journal of Marine Research, Vol. 14,
          No. 3, PP. 211-216, 1965.
14.       Palmer, C. N., The Effect of Pollution on Rive? Algae, New
          York Academy of Sciences, Vol. 108, Article 2, pp. 389-395,
          June 29, 1963*
                                  -38-

-------
APPENDIX
 - 39 -

-------
                              TABLE 1

          BIOLOGICAL SAMPLING STATIONS AND REFERENCE POINTS

                  MERRIMACK RIVER AND TRIBUTARIES


STATION    RIVER MILE    DESCRIPTION

   1       115.70-0.U6  Pemigewasset River, k ft. depth off east bank-
                        1000 ft. upstream of large rock near confluence
                        with Winnepesaukee River.

   2       115.70-0.19  Winnepesaukee River, 3 ft. depth off south bank-
                        1000 ft. upstream of big rock near confluence
                        with Pemigewasset River.

           115.70       Confluence of Pemigewasset and Winnepesaukee Rivers.

   3       115.53       Merrimack River, 3 ft. depth off east bank- 1000  ft.
                        downstream of confluence of Pemigewasset and
                        Winnepesaukee Rivers.

   k       Ilk.Ok       1 ft. depth off west bank downstream of Franklin, N.H.

   5       113.53       k ft. depth in  midstream channel at Daniel Webster
                        Island.

   6       112.60       3 ft. depth off west bank 1 mile downstream of
                        Daniel Webster  Island.

   7       111.18       k ft. depth off west bank under high tension wires.

   8       102.Ok       1 ft. depth off east bank 1000 ft. upstream of
                        White Tower.

   9       100.71-0.5    Contoocook River, 6 ft. depth, off south bank 500 ft.
                        downstream of R.R. bridge below tannery.

           100.71       Confluence with Contoocook River.

            97.83       Sewells Falls Dam.

  10        95.30       k ft. depth off north bank 1 mile upstream of Iron
                        bridge above Concord.

  11        93.38       2 ft. depth, 1  mile downstream Rt. 3B bridge.


                               - A-l -

-------
                         TABLE  1 (Continued)


STATION     RIVER MILE   DESCRIPTION

             91.60       U.  S.  Route k & 202  Bridge,  Concord,  N.  H.

  12         91.51       10  ft. depth off west bank,  100 ft. downstream
                         k ft.  diameter outfall.

             87.61       Confluence with Turkey River.

             86.80       Garvins Palls Dam.

  13         86.60       8 ft.  depth,  1/5 mile downstream from Garvins
                         Falls  Dam.

  Ik         86.30       1 ft.  depth in midstream,  off  sandbar 1/2 mile
                         downstream of Garvins Falls  Dam.

  15         85.80-0.Ok  Soucook River, 2 ft.  depth off north  bank 200  ft.
                         upstream of confluence with  Merrimack River.

             85.80       Confluence with Soucook River.

  16         Qk.QO       10  ft. depth off east bank,  1  mile downstream  of
                         Soucook River.

  17         83.68       k ft.  depth off west bank  near Bow Bog Brook.

  18         82.90-0.2   Suncook River, 3 ft.  depth,  midstream, 100  ft.
                         downstream of 5 ft.  cement outfall.

             82.90       Confluence with Suncook River.

  19         81.2k       k ft.  depth off east bank, 1000 ft. upstream of
                         HooksetbDam.

             81.05       Hooksett Dam.

  20         80.55       10  ft. depth off west bank,  100 ft. downstream
                         of  R.R. bridge.

  21         77.60       8 ft.  depth off east bank, 3 miles downstream
                         of  Hooksett,  N. H.

  22         76.79       8 ft.  depth under 1st high tension wires downstream
                         of  Hookset Dam.

  23         7^.17       8 ft.  depth off east bank, 20  ft. downstream of
                         outfall opposite k radio towers.


                                - A-2 -

-------
                         TABLE 1 (Continued)


STATION     RIVER MILE   DESCRIPTION

             73.Ik       Amoskeag Dam, Manchester,  N. H.

             71.30       Confluence with Piscataquog River.

  2k         68.05       1 ft. depth off east bank, 200 ft. upstream of
                         R.R. bridge.

  25         65.11       1 ft. depth off east bank  under  high tension
                         wires, about 3 miles downstream of Goffs Falls.

             62.35       Confluence with Souhegan River.

  26         59.28       k ft. depth off west -bank, 1 mile  below Nesenkeag
                         Brook.

  27         58.29       5 ft. depth off east bank, 0.36  mile below Little
                         Nesenkeag Brook.

  28         58.10       6 ft. depth in midstream at Rodonis1  Farm.

  29         57.91       6 ft. depth, 1000  feet below Rodonis1 Farm.

  30         57.10       k ft. depth, midstream,  0.65 mile  below Pennichuck
                         Brook.

  31         55.75       k ft. depth off east bank  at high  tension wires
                         about 1 mile upstream of Hudson  Bridge.

  32         5^.80-0.01  Nashua River, 1 ft. depth, midstream, 50 feet
                         upstream of confluence with Merrimack River.

  33         $k.QO-k.O   Nashua River, k ft. depth  off south bank of canal,
                         10 ft. upstream of Rt. 3 bridge.

             5*1.80       Confluence with Nashua River.

  3k         5^.25       5 ft. depth off east bank, 100 ft.  downstream  of
                         Twin Piers below Hudson.

  35         52.81       7 ft. depth, midstream 500 ft. upstream of  high
                         tension wires.

  36         52.72       Under high tension wires.

  37         52.53       5 ft. depth, 1000  feet downstream  of high tension
                         wires.


                                - A-3 -

-------
TABLE 1 (Continued)
STATION
-
38
39
to
1*1
1*2
1*3
Ul*
1*5
-
1*6
1*7
1*8
-
-
!*9
50
51
52
53
54
RIVER MILE
1*9.82
1*8.95
1*8.76
U8.57
1*7.51*
1*7.35
1*7.16
1*6.82
1*4.69
1*3.1*7
1*3.1*7
1*2.52
1*2.22
1*0.60
38.75
37.1*5
36.89
36.36
36.30
35.11
31*. 48
DESCRIPTION
New Hampshire -Massachusetts state line.
7 ft. depth, 1000 ft. upstream of Lakeview Avenue.
7 ft. depth, at Lakeview Avenue.
8 ft. depth, 1000 ft. downstream of Lakeview Avenue.
10 ft. depth, 1000 ft. upstream of Tyngsboro Bridge.
10 ft. depth, Tyngsboro Bridge.
10 ft. depth, 1000 feet downstream of Tyngsboro Bridge
10 ft. depth, below power lines.
9 ft. depth, 200 feet downstream of Tyngsboro
Island and small channel.
Lowell Water Intake.
1* ft. depth, 50 feet downstream of Deep Brook.
10 ft. depth, 50 feet downstream of power line.
10 ft. depth, off north bank, near Lowell Drive -In.
Pawtucketvllle Dam, Lowell, Mass.
Confluence with Concord River.
Below Duck Island.
1* ft. depth, off south bank 100 ft. downstream
of gas line crossing.
7 ft. depth, off north bank- 15 ft. downstream of
Richardson Creek culvert.
9 ft. depth, at midstream 300 ft. downstream of
culvert-Richardson Creek-near golf course.
200 yards upstream of power lines.
150 yards upstream of Dracut-Methuen line.
       - A-l* -

-------
TABLE 1 (Continued)
STATION
55
56
-
57
58
59
60
-
-
61
-
-
62
63
64
65
66
67
-
68
RIVER MILE
3^.39
33.93
33.03
32.37
31-92
31.7^
31.66
29.81
28.99
28.50
27.85
27.^5
25.35
23.^3
21.85
21.15
19.62
19-35
18.85
17.75
DESCRIPTION
Dracut-Methuen line.
100 yards upstream of used car lot near Wheeler St.
Confluence with Fish Brook.
Drive-In Theater, Methuen.
At Mill Pond Brook (Bartlett Brook) off north bank.
Upstream end of Pine Island.
100 yards upstream of Interstate 93 bridge.
Lawrence Water Intake.
Essex Dam, Lawrence, Mass.
3 ft. depth, off east bank, 1/2 mile downstream
of Essex Dam.
Confluence with Spickett River.
Confluence with Shawsheen River.
k ft. depth, off north bank, opposite Western
Electric outfall.
k ft. depth, off east bank, upstream of Kimball
Island.
k ft. depth, off south bank, opposite Creek Brook.
k ft. depth, off south bank, opposite Stanley
Island.
k ft. depth, off south bank, opposite Moody School.
5 ft. depth, off north bank, 1000 ft. upstream of
Washington St. and Rt. 113 bridge.
Confluence with Little River.
5 ft. depth, off north bank, 200 ft. below outfall
of Hale Hospital.





       - A-5 -

-------
                      TABLE  1  (Continued)
STATION
69
70
71
72
73
74
75
76
77
78
79
80
81
.82
83
RIVER MILE
17.30
16.56
16.51
16.17
16. lit
16.03
15.87
15.68
14.00
12.50
8.81
7.80
7.28
5.00
4.10
DESCRIPTION
3 ft. depth, off upstream end of Porter Island.
8 ft. depth, midway between buoys 60 & 6l.
5 ft. depth, off south bank, opposite downstream
tip of Porter Island.
6 ft. depth, line between Johnson's Creek &
Buoy #60-upstream.
5 ft. depth, line between Johnson's Creek &
Buoy #60-downstream.
3 ft. depth, on line between dry creek and Buoy #58
10 ft. depth, midway between Groveland Bridge
& Buoy #57.
300 yards downstream of Groveland Bridge.
5 ft. depth, off south bank, about 2 1/4 miles
upstream of Rocks Village Bridge.
4 ft. depth, off south bank, 3/4 mile upstream
of Rocks Village Bridge.
5 ft. depth, off south bank, 500 ft. upstream of
confluence with Indian River.
6 ft. depth, off south bank, 200 ft. upstream
of confluence with Artichoke River.
5 ft. depth, off south bank, 2000 ft. upstream
of Bailey Pond.
Off west bank Eagle Island.
4 ft. depth, off north bank, opposite mid-point
                       Carr Island.

84          3.40       6 ft. depth,  off east bank 1/2 mile upstream of
                       R.R. bridge.

85          2.28       2 ft. depth,  off north bank 100 yards downstream
                       of power lines.
                              - A-6 -

-------
                         TABLE 1 (Continued)


STATION    RIVER MILE    DESCRIPTION
86
87
88
89
90
91
92
93
2.20
2.1?
2.15
1.84
1.73
0.98
0.90
0.46-0.5
10 ft. depth, 50 yards downstream of Newburyport
sewage outfall.
10 ft. depth, in channel, on line between
Buoys 13A and 14.
5 ft. depth, off south bank, 500
of Newburyport sewage outfall.
3 ft. depth, off south bank, 700
of Newburyport sewage outfall.
6 ft. depth, 1/2 mile downstream
sewage outfall.
ft. downstream
yards downstream
of Newburyport
5 ft. depth, 100 feet offshore, opposite Shad Creek
5 ft. depth, just west of Woodbridge Island.
3 ft. depth in Black Rock Creek.

              0.46       Confluence with Black Rock Creek.

  94          0.15-1.15  Plum Island River, 5 ft. depth, off east bank of
                         little  island between Woodbridge and Seal Island.

              0.15       Confluence with Plum Island River and the Basin.

  95          0.15-0.53  In Basin, 5 ft. depth, 200 ft. from south bank.
                                - A-7  -

-------
oo
                                                       TABLE 2

                                     NUMBER OF BOTTOM ORGANISMS PER SQUARE METER

                                                    WHITMAN RIVER
                                                     (June
KINDS      t

Beetles
     Elmidae
          Stenelmis sp.
          Promoresia sp.
     Psephenidae
          Psephenus herricki

Caddis flies
     Leptoceridae
          Leptocerus sp.
     Limnephilidae
          Limnephilus sp.
          Neophylax sp.
     Hydropsychidae
          Macronemum sp.
          Smicridea sp.
     Rhyacophilidae
          Rhyacophila  sp.
                               SENSITIVE ORGANISMS
              SUBTOTAL ORGANISMS
             SUBTOTAL KINDS
                                       129
                                        11

                                        11
                                        32

                                        11
                                        5^

                                        ^3
                                        11

                                        5l6
    Stone flies - Taeniopteryginae
              Brachyptera &p.            172

    May flies - Heptageniidae
              Iron sp.                   65
              Stenonema sp.              22
                                   1077
                                     12
KINDS

Beetles - Haliplidae
          Haliplus sp.

Moth flies - Psychodidae
          Pericoma sp.

Midge flies - Tendipedidae
          Pentaneura sp.
          Procladius sp.
          Cryptochironomus sp,

Clams - Sphaeriidae
          Pisidium sp.

          SUBTOTAL ORGANISMS
          SUBTOTAL KINDS
INTERMEDIATE ORGANISMS
                                                                                                     11
           850
           850
           161
            11
            65

          19^8
             6
                                                    Sludge worms - Tubificidae
                                                              tubificids without gills

                                                              SUBTOTAL ORGANISMS
                                                              SUBTOTAL KINDS
                                    TOLERANT ORGANISMS
                                             22

                                             22
                                               1
          GRAND TOTAL ORGANISMS
          GRAND TOTAL KINDS
          30^7
             19

-------
                                                                                                                                                                  TABLE 3

                                                                                                                                               KINDS OP BOTTOM ORGANISMS IN MERMMACK RIVER

                                                                                                                                                       AND NUMBERS PER SQUARE METER
STATION NO.
                                                                                                             11
                                                                                                                         13 .   1U    15    16    17    18    19
                                                                                                                                                                         21
23    24
            25
26    27    28    29    30    31    32    33   34    35    36    37    38    39    l»o    la    Ita    It3    Wi    45    46
                                                                                                                                                                                                                                                                                                                                    47
SKNSlTlVli ORGANISE


     Caddiafliea - Tricbpptera
          Helicopsychidae sp.
          Leptoceridae «pi
          T.^^p«aph< Hdae SP.

     Riffle beetles - Elmldae
          Steneljaia ap.

               Subtotal Organis
               Subtotal Kinds
INTERMEDIATE ORGANISMS


      Biting midges  - Releinae
           BeKia sp^

      Clan - Sphaeriidae
           Piaidim  ap.
      Cranefliea - Tlpnlidae
                            ap.
      Dragon fllea - Anisoptera



      Midge fllea - Tendipedidae
           Brillia, ap.
           Calopaeetra ap.

           Glyototendipea aenllla
                        IBs.
           	
           Pentaneura nelanopa
           Polypediliaa convletua
                 ""
           Tanytarsua nigricana
           Tanytaraua aubtendena

      Hnaaela - Unionidae
           Blllptio ap.
           Unioaems ap.

      Scavenger Beetles - Bydrophllldae


      Scuds - Aaphipoda
           Hyalella aiteca

      Sow Bugs - Isopoda
      Snails
           BnllMldae
                AmicolA ap.
           Phyaldae
                Phyma ap.
                Bellsoaa ap.
                Ojraulua sp»
           TlTlparldae
                Caapeloaa ap.
                     Subtotal Organlaaa
                     Subtotal Kinds
 TOIZRANT OBGAmSMS
      Leeehea - Sloaaipbonlldae
           Qlosaiphonia heterocllta
           Helobdella
           Helobdella punetata - lineata
           Helobdella s^fp-n-
           Plaeobdella paraaitlea

      Nidge fides - Tendipedidae
           Glyptotendipee lobiferua
                  a antliraclnua
      Slodgevom - Tubifieidae
           Tubificida nitbont gllln

                     Subtotal Organiaaa
                     Subtotal Kinds

                          Grand Total Organiaaa
                          Grand Total Kind*




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635
                                                                                                                                                                                                                                                            - A-9-

-------
                                                                                                                                                       TABLE  3  (Continued)

                                                                                                                                           KINDS OF BOTTOM ORGANISMS IN MEKUMACK IUVEF

                                                                                                                                                   AND NUMBERS  PER SQUARE METER
STATION NO.
50    51    52    53    5U     55     56    57    58    59    60    61    62    63    6U    65    66    67    68    69    70    71    72    73    71*    75    76    77    78    79    80    81    82    83    81*    85    86    87    88    89    90    91    92    93    9"<    95
SENSITIVE ORGANISMS
     None
IMTERMEDIATE ORGANISMS
     Barnacles  - Balanonorpha
      Clams
          Myacidae
                Mya arenaria
          Sphaerlidae
                Pisidiun sp.

      Midge  flies - Tendipedidae  ..
          Cryptochironomus sp.
          Kndochironoms subtendens
          Gljptotendipes aeniUs
          Polypedllxm sp.
          ProelBdius sp.

      Mudcrabs - Pilvramidae
          Bhithropanopeus h»i-ri «<

      Mussels - Mytilidae
          Mytilus edulls

      Scuds
           Ganmaridae
                Ganmarus fasciatus
                Gammarus locusts

           Pontogeneiidae
                Pontogeneia inermis

      Shrink-like animals - Callianassidae
           Callianassa atlantica

      Sow Bugs
           Anthuridae
                Cyathura carinata

           Janiridae
                Jaera marina

      Snails
           Bulimidae
                Amnicola sp.
           Rissoidae
                    obia minuta
                     Subtotal Organisms
                     Subtotal Kinds
 TOIfflANT ORGANISMS

      Leeches - Glossiphoniidae
           Helobdella fusca
           Helobdella stagnalis

      Marine Worms - Nereidae
           Nereis sp.

      Midge flies - Tendipedidae
           Glyptotendlpes lobiferus
           Tendipes anthraeinus

      Sludgewonns - Tubificidae
           Tubificids without gills

                     Subtotal Organisms
                     Subtotal Kinds
                          Grand Total Organ!s
                          Grand Total Kinds




—
—










0
0






21OU
2110
2
2110
2




~
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0
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71
79
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79
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8
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115
173
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197
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16
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299
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281*
331
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331
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8
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77
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614
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146
61
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1939
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1182
1332
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1955
3
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173
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630


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32

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11*972
15508
3
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107
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168






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123
507
2
675
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31
31
1
92
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92
92
1
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1»30
l4l»5
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16
16
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169






230







107
1
337
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693






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536


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1153
lj
                                                                                                                                                                                                                                                                         - A-10 -

-------
                              TABLE k

                        SOUHEGAN RIVER MILES
BIOLOGICAL
  SAMPLE
  NUMBER
   S-l
   S-2

   S-3

   S-lf
   S-5
   S-6
   S-7

   S-8
   8-9
   S-10
   S-ll

   S-12

   S-13

   S-lU
RIVER
MTT.E

28.58
21. k6
21M
21.1*2
20. 15-1.
20.15
18.17
15.58
13.31
11.82
10.60
 8.te
 8.1*0
 6.80
 6.53
 6.51
 3. 11*
 3
   12
 1.15
 0.73
 0.70
 0.3U
 0.00
              LOCATION
                           Rte.  31 Bridge, Greenville
              Rte. 31-101 Bridge, Wilton
              Stony Brook
              Confluence vdth Stony Brook, Wilton
              North Purgatory Road Bridge, Milford
              Confluence with Tucker Brook, Milford
Rte. 13-101 Bridge, Milford
Riverside Cemetery, Milford
Ponemah Bridge, Amherst

Honey Pot Pond Bridge, Amherst
Amherst-Merrimack Town line
Severns Bridge, Merrimack

Turkey Hill Bridge, Merrimack

USCG Gaging Station, Merrimack

Everett Turnpike Bridge, Merrimack

U. S. Route 3 Bridge, Merrimack
Confluence with Merrimack River (mile 62.35)
                                - A-ll -

-------
                                                                             TABU 5
                                                                    KZHDS OP BOTTOM



                                                                      IN SOtHBQAK RIW ABB




                                                                    AUMBBtS PB 89MB KBIM
STATION NO.
                                         8-1   B-2   B-3   S-l»   8-5   8-6   8-7   8*8  8-9  8-1O  8-11  8-12  8-13  8-
Beetles
Elald
, Pseph
t
Caddis fli
a:
M
R
I
•oaor
vpho
eg
mil
a**"7|i-<-
snidae
es - Trie
ilossosei
lelicovn
a gpj.
i bnrieki
JMptara
ftflU.
rcb« SP.
ids*
L; Ban £ , ; dae
Molanna gp.
Fish flies - Megoloptera
Chauliodes sp.
May flies - Bphemoptera
Aneletus gp.
Par"! •ptophlebia gp.
Stoneflies - Plecoptera
Isoperla gp.
Subtotal Organigns
Subtotal Kinds
CTTB1MPIAIE ORQAHIfflffi

Beetles
Haliplldae
Brychius gp.
Hydrophilidae
Bjfdrochus gp.
Clams - Sphaeriidae
Museuliua gp.
Fialiina gp.
Crane flies - Tipulldae
Antocha gp.
Damsel flies - Zygoptera
Igchimra SP.
Dragon flies - Anisoptera
(kmpnus sp.
Midge flies - Tendipedidae
Brillia gp.
Cricotopus gp.
1
]
nooenii^o
Ql?nitoteni
I
in
iroba
«a

lipes lobiferus
OM SP.
Metrioenamug fuscipea
KlerotendJ-pes gp.
Polraedllum f allaz
Proeladius gp.
Mussels - Unionidae
Lamp sills gp.
Scuds - Amphipoda
Hyallela azteca
Snails
Bulimidae
Fl&norbidae
Heliscam SB.
TiTipariidae
Campeloaa gp.
Sow Bugs - Isopoda
Asellus militaris
Water Boatmen - Corixldae
Sigora sp.
Subtotal Organisms
Subtotal Kinds
TOiatAHT ORSAinSK
B



Leeches - Qlossiphoniidae
QlosBiphonia heteroclita
Helobdel " Tnmetata-lineata
Helobdel
Placobde

Midge flies - Tendipedidae
Tendipes anthraeinua
Sludgeworms - Tubificidae
Tubiflcids
Subtotal Organisms
Subtotal Kinds
Grand Total, Organisms
Grand Total Kinds
183
97
6k
11
118
5k
22
108
711
9
„_

22
~




-.


.t3

22
n

*
—

75
75
1
8oU
lU
64
172
:*
129
u.
1*3
83e9
—
97
—




—


~

8

«


3
—

366
366
1
1*09
12
U
75
iko
s
237
5k
97
11
226
183
22
850
11
U
n
2036
16
32
22
97
5k
22




32


~

22
~

~

281
7
<••

U3
1*3
1
2360
24

22
—
-.



32
—
—

97
3
__

—









—

—
~

11
32

2
11
2190

333
3
267k
8

"•

~
~



172
—

11


183
2
„

— —
11









11

~
~

11
22
32

130
6

2280

881
3l6l
2
10

11

~
_



—
—





n
i
_—

»
~













~
—

—

0
0

118

MB
118
1
129
2

-

—
«



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—





0
0



32
~









5k


—
—

118
22
366

592
5

—

iiuo
nUo
1
1732
6

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—
32

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1*3
2



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~









22


k3
—

11
32

108
k
108
2*7

151
506
3
657

~

~
108



11
~





119
2



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~

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75
183
11

269
3
6k
730
32
387
1013
k
1601
9
11
22
11

11
32
11


86
—





I8lf
7



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n

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11


301


388
5

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323
387
753
3
1325
15
— —

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5k


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—





65
2



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—

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u


—


11
*


891*
5

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1*3-
1
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11

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32


22
—



^ , _
65
3


22
U
11
--



129
~


~
~
215
388
5

11
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1
*$
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-
32

32
11
—



— ^
75
3


11
-
--

^
U
11
22
11
75
22
5*
U
22
250
10

~
150
366
516
Z
15
—


H3

U
—



..
2


75
22
—
22
32
191*
32
376

—
22
75
U
86
1033

32
75
365
ktz
3
1559
18

-------
                                                                      TABLE 6

                                                   EROBUCTIVrrY OF MERRIMACK RIVER - AUGUST 1965
  Date
Aug. 1965
   18
   19
   20
   A
   25
   26

AVERAGE
   18
   19
   20

   25
   26
   27

AVERAGE
   18
   19
   20
   2k
   25
   26
   27

AVERAGE
Op Produced
 gm/nP/day
   0.576
   0.0 -
   1.140
     979
   0.1*50
   0.629
               5.33
               3.72
               3.55
               6.53
               7.82
               2.18
               1.25

               4.34
   2.83
   3.16
   4.34
   6.05
   7.57
   4.52
   4.15

   4.66
7.30
3.65
3.05
1.00
0.70
1.20

2.82
                  40.8
                  21.4
                  12.1
                  15.6
                  11.2
                  14.6
                  19.3
32.7
32.0
17.3
17.8
25.2
23.9
24.8
               Algae
            No. x 10b/n>3
 233.4
 241.2
 206.8
 109.5
 116.4
  60.2

 161.2
              1230
               938
               507
              1252
               475
               186
               765
 731
 494
 555
1488
1815
 779
 977
                                  N03 + NO,
             River Flow   NHo-N   Nitrogen"
             106 CF/day   mg/1      mg/1
                                                                                          Total N
82.7
                                                                  RIVER MILE 65.11
0.48
0.64
                                                                  RIVER MILE 48.76
95.0
103.7
99.4
96.8
93.3
86.4
0.44
0.66
0.6l
0.66
0.86
0.82
0.40
0.50
0.50
0.60
0.80
0.70
                95.8      0.67      0.58

                      RIVER MILE 43.47
1.67
                                                  35
                                                  69
                                                  45
                                                  86
                                                  20
                                                2.10
                                1.77
                                        Ortho-POU
                                          mg/1
87.3
78.6
72.6
82.9
88.1
86.4
0.51
0.49
0.30
0.53
0.53
0.49
0.60
0.50
0.55
0.80
0.80
0.60
1.55
1.41
1.46
2.13
1.92
1.58
0.36
0.42
0.32
0.30
0.29
0.25
                                                                                                      0.32
                                          0.61
                                          0.63
                                          0.64
                                          0.54
                                          0.64
                                          0.51
                                0.60
                                          Total
                                            mg/1
0.77
0.53
0.37
0.46
0.39
0.34

0.48
                                            0.74
                                            0.73
                                            0.78
                                            0.72
                                            0.77
                                            0.52
                                  0.71
99-3
108.9
106.3
95.9
86.4
86.4
0.36
0.49
0.49
0.69
0.74
0.66
0.50
0.40
0.45
0.60
0.80
0.60
1.37
1.17
1.95
—
1.90
1.86
0.44
0.55
0.53
0.49
0.50
0.50
0.58
0.73
0.69
0.59
0.59
0.64
97.2
0.57
0.56
1.65
                                                                                                      0.50
0.64
                                            Turbidity
                                              mg/1
6.0
1.8
2.0
2.5
2.3
1.6

2.7
                                              4.3
                                              3.9
                                              2.5
                                              3.0
                                              2.8
                                              2.6
                                                        3.2
                                                                  4.5
                                                                  3.6
                                                                  2.7
                                                                  3.5
                                                                  6.0
                                                                  2.0
                                                                                                                              3.7
                                            Solar Radiation
                                              gm cal/cm2
314
220
338
464
488
298

354
                                                 314
                                                 220
                                                 338
                                                 464
                                                 488
                                                 298
                                                 354
                                                                       314
                                                                       220
                                                                       338
                                                                       464
                                                                       488
                                                                       298
               354
   NOTES:  Nitrogen and phosphorus represent soluble forms.
           All samples taken at one foot depth.

-------
                                                             TABLE 7

                                        MDST ABUNDANT GENERA OF ALOA1 IN MSRIMACK RIVER

                                                       APRIL-OCTOBER, 1965
DIATOMS
     Asterionella
     Melosira
     Synedra
     I ivicula
     Other
          TOTAL ASU/ml

BLUE-GREEN
     Anabaena
     Polycystis
     Coelosphaeiun.
     Cscillatoria
     Other
          TOTAL ASU/ml

GREEH
     Protococoua
     Pediaatrum
     Scened«smus
     Eudorina
     Pandorina
     Other
          TOTAL ASU/ml

FLAGELLATES
     Dinobryon
     Chlamydomonas
     Synura
     Mallononas
     Eugiena
     Other
          TOTAL ASU/ml

9
455
—
40
Kf\
J\J
5U3


.-
--
0
„

20
—
—
—
20
375
1*5
200

.60
APRIL
16
100
40
30
50
10
230


_.
--
0
„

30
—
—
—
30
30
20
30
in
xw
20

23
130
70
10
30
on
j*-'
270


20
--
20
..

10
--
—
—
10
20
60
50

40

14
240
20
395
60
90
805


__
--
0
„

40
100
100
20
260
400
1*5
150

~
MAY
21
50
90
3K>
30
10
490


__
--
0
..

?0
—
—
80
130
285
50
225

~

28
160
325
250
20
__
755


20
50
70
550

60
—
-.
130
740
200
150
140

20

7
40
130
210
—
30
530


__
--
0
360
Ort
40
—
20
170
620
80
55
50

20
JUNK
11
150
260
90
10
i fin
±\j\j
30
640

'
__
—
250
820
on
30
..
_.
360
1300
„
80
30

10

181
90
40
190
40
._
500

en
?w
~
50
325
ion
j~c.\j
1*0
—
—
80
665
250
75
—

~

6
46
—
1277
—
__
1323


342
—
342
„
1 1 ll
410
1300
—
1915
3739
„
548
342
n4
J_LH
JD
12
..
700
40
—
— „,
740
Ron
(_nju
3400
300
4500
„
liAft
*tw
1370
300
1000
340.
3410
__
260
—

9£r>
LY
14
40
630
—
—
20
690


—
--

..
Aiin
570
—
300
1100
2610
..
40
200
on
c\J

23
„
870
100
—
__
970


.-
--
c
1280
T2r\
490
100
—
790
338o
„
340
—
On
O\J
40
SEP
29
„
97
--
281
19
397


__
--
0
194

272
291
—
174
931
„
97


•so
OCT
29
58
—
19
19
_„
96


—
--
0
..

281
97
-.
911
1289
78
39
194
1 3.1*
~
'780   110   170   695   560   510   205   120   325   1004   520    260   460   136   447
     GRAND TOTAL
     ASU/ml
1345   370   470  1760  1180  2075  1355   2310  1540 64o8   9170  3560   4810  1464  1S32
                                                               - A-14 -

-------
                                                             TABLE 8

                                     MOST ABUNDANT GENERA OF ZOOPLANKTON IK MERRIMACK RIVER

                                                       APRIL-OCTOBER, 1965
CILIATES
     Codonella
     /orticella
     Other
          TOTAL #/20 ml

ROTIFERS
     Polyarthra
     Anuraea
     Synchaeta
     Triarthra
     Other
          TOTAL #/20 ml

CRUSTACEA
     Bosmina
     Cyclops
     Daphnia
     Nauplius
     Other
          TOTAL #/20 ml

          CSRAND TOTAL, #/20 ml
          ZOOPLANKTON

          AMORPHOUS MATTER,
          ASU/ml x 103

          WATER TEMP. °C         —    —  9.5    9.2   17.2   20.1*     20   23.3   20.2   2l*.2    28    25    Zk    19  9-7
AmU MAY
Q 16 23 11* 21
— 100
0 0 0 0 100

1



00001





00001
0 0 0 0 102
1.2 1 2.25 10 10

28
—
0

6



8





0
8
1.5
JUNE
7 11 18 6
5 - 2 -
25 2 2 0

10 10 25



10 10 28 18



,

0 k 0 1
35 16 30 19
352 2.85

12
—
0

10
12
k

27
7


2

9
36
2
JULY
Ifc 23
__
0 0

1»0
— — 1
2
12 --
12 43

5



5 12
17 55
2 1.25
SEP
29
1
9
10
8
13
3

Q
33

1

2

3
1*6
7.8
XT
29
1*008
1*008

2
_—
••
1
3





0
U011
3.49
                                                             - A-15 -

-------
MERRIMACK  RIVER  BASIN
                                       4	4	t

-------