COMPARATIVE STUDY
                        on
                   THE EUTROPHICATION
                        of
                  LAKE SEBASTICOOK, MAINE
                    1965, 1971-1973
     United  States
     Environmental
     Protection  Agency
     Region I
JOHN F. KENNEDY FEDERAL BUILDING - GOVERNMENT CENTER - BOSTON, MASSACHUSETTS 02203

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        COMPARATIVE STUDY
                on
        THE EUTRQPHICATION
                of
      LAKE SEBASTICOOK, MAINE
          1965, 1971-1973
               by
          P. M. Nolan
         A. F. Johnson
U, S. ENVIRONMENTAL PROTECTION AGENCY
              REGION I
        BOSTON, MASSACHUSETTS

              APRIL 1975

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         LAKE SEBASTICOOK,  MAINE
U. S. ENVIRONMENTAL PROTECTION AGENCY




              REGION I




        BOSTON, MASSACHUSETTS

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                          TABLE OF CONTENTS

                                                                 Page

SUMMARY                                                           1

CONCLUSIONS                                                       3

INTRODUCTION                                                      4

  History                                                         4
  Conduct of Study                                                5
  Sampling Procedures                                             5

BIOLOGY                                                           8

  Benthic Invertebrates                                           8
  Algae                                                          10
  Chlorophyll a_                                                  12

NUTRIENTS                                                        13

  Summary (Nitrogen and Phosphorus)                              13
  Sediments                                                      18
  Nitrogen/Phosphorus ratios                                     19

  Discussion (Nutrients)                                         19

  vertical Profiles,. Cha, pH, Temp., D.O.                        24

RECOMMENDATIONS                                                  25

BIBLIOGRAPHY                                                     27,  28

TABLES                                                          - 29 - 40

APPENDIX                                                         41,  42-A-l-
                                                                 67 A-26

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                           LIST OF TABLES
Table                          Title                             Page^

  1               Lake Sebasticook,  Maine - Sampling                29
                   Stations 1965 - 1973

  2               Benthic Organisms  - Lake Sebasticook,             30,  31
                   Maine - May 31, 1972

  3               Benthic Organisms  - Lake Sebasticook,             32
                   Maine - May 30, 1973

  4               Benthic Organisms  - Lake Sebasticook,             33
                   and Lake Wassookeag, Maine
                   July 31 - Aug.  1, 1973

  5               Benthic Organisms  - Lake Sebasticook,             34
                   Maine - Nov. 17,  1971

  6               Midgeflies - Sludgeworms Average  Per Sq.          35
                   Ft. Lake Sebasticook, Maine 1965 -  1973

  7               Lake Sebasticook,  Maine - Algae Count             36
                   (Average) Per Milliter - 1965 -  1973

  8               East Branch Sebasticook River, Maine             37
                   Algae Count Per Milliter
                   1965, 1971 - 1973 (Comparable Stations)

  9               Lake Sebasticook,  Maine                          38
                   Chlorophyll a_,  Micrograms Per Liter

  10              Lake Sebasticook,  Maine                          39
                   Sediments Nitrogen/Phosphorus Ratios

  11              Lake Sebasticook,  Maine                          40
                   Sediments, Phosphorus and Nitrogen
                   %  Dry Weight
                                   11

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                        LIST OF FIGURES
Figure                       Title                              Page

  1                 Lake Sebasticook Sampling Station             6
                     Locations - 1965 and 1973

  2                 Lake Sebasticook Tributaries                  7
                     Sampling Station Locations
                     1965 and 1973

  3                 Seasonal Averages -  Lake  Sebasticook,         15
                     Maine - Total Phosphorus, Total
                     Kjeldahl Nitrogen

  4                 East Branch Sebasticook River  -               16
                     Seasonal Average Total Phosphorus

  5                 East Branch Sebasticook River                 17
                     Seasonal Average Total Kjeldahl
                     Nitrogen

  6                 Lake Sebasticook - July 31, Aug. 1,           26
                     1973 Mean Chlorophyll a_   - pH -
                     Temperature - Dissolved  Oxygen Vs.
                     Mean Water Depth
                                 111

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                             SUMMARY







     A technical study was conducted from November 1971 to August 1973




to assess eutrophication of Lake Sebasticook at Newport, Maine and to




measure progress versus work conducted in 1965.  Selected biological




and chemical parameters are compared on a seasonal and yearly basis.






     Average total phosphorus values are higher during the 1971-1973




survey than during 1965.  Station EB-01 at the outlet of Corundel Lake




(Station 3) is of special interest because of the higher total phos-




phorus average of 0.15 mg/1 compared to 0.07 mg/1 average of 1965.




This indicates the possibility of the discharge of additional municipal




and/or industrial waste from the Dexter vicinity or a non point dis-




charge since 1965.  Total phosphorus concentration has increased in




Lake Sebasticook to approximately 0.08 mg/1 in 1971-1972 and approxi-




mately 0.10 for 1973, up from 0.05 mg/1 in 1965.  This is considered




hypereutrophic according to recent data from the National Eutrophication




Survey.






     Less nitrogen is present in the inlet water of the East Branch of




the Sebasticook River and in the water column and sediment of Lake




Sebasticook now than in 1965.  Denitrification of aquatic systems is




known to occur when photosynthetic activity of algae causes high




alkaline levels in warm eutrophic lakes, and sediments have low con-




centrations of oxygen.  However, denitrification is a complex process




that is still inadequately understood and its explanation is not




attempted in this report.
                              -1-

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     Two kinds of pollution tolerant benthic fauna,  tubificid worms




and midgefly larvae, were dominant in 1972 and 1973  as in 1965.




Further degradation of bottom sediments is indicated by the smaller




number of pollution tolerant organisms present in 1972 (14 per sq.




ft.) and in 1973 (3 per sq. ft.) compared to 1965 (35 per sq. ft.).






     Excessive algal growths similar to the 1965 eutrophic conditions




for Lake Sebasticook are indicated when overall average chlorophyll a




values and algal counts for 1971 to 1973 of 11.26 ug/1 and 11,715




cells/ml respectively are compared with 11.63 ug/1 chlorophyll a_ and




11,354 cells/ml for 1965.  The extent of enrichment is apparent when




Sebasticook is compared to the nonfertilized water of Lake Wassookeag




with a 1973 chlorophyll -a value of 0.38 ug/1 and cell counts of




467/ml.
                                 -2-

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Conclusions




     1.  Phosphorus input to Lake Sebasticook from the East Branch of




the Sebasticook River is still too high to reverse Sebasticook  from  a




eutrophic state to a non-eutrophic state.






     2.  Lake bed sediments and riverbed sludges  are  a significant




source of phosphorus in Sebasticook.  -






     3.  Nitrogen overall is lower within the immediate  Sebasticook




system and the data suggests a denitrification process within the lake.






     4.  Benthic animal populations are sparse and consist only of




sludge worms and red midges.






     5.  Algae continues to be a problem,  however, some  seasonal




improvements are noted.






     6.  All parameters taken collectively describe Lake Sebasticook




as eutrophic.

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                          INTRODUCTION









History




     Lake Sebasticook at Newport, Maine was studied in 1965 by




Mackenthun 1966 Get al) to determine the extent and cause of




excessive fertilization of the lake.  Eutrophication of the lake was




confirmed by the volume of blue-green algae, noxious odors, low or




absent dissolved oxygen in the deeper waters, high phosphorus and




nitrogen concentrations and a lake bed associated animal population




composed dominantly of midge larvae and sludgeworms.  Fertilization




of Lake Sebasticook was determined to be caused principally by




nutrients contained in domestic and industrial wastes discharged to




the East Branch of the Sebasticook River at Dexter and Corinna,




Maine.<1} <2)






     Subsequent to the 1965 study one major source of phosphorus




pollution,  a potato processing plant, to Lake Sebasticook burned down.




The town of Corinna constructed and now has in operation a secondary




waste treatment plant  (WWTP) with a designed level of treatment




capable of 85-95% BOD and solids removal.  Phosphorus removal up to




30% can be expected from a plant of this design.  The WWTP is designed




to receive a volume of 1.2 mgd which breaks down to 1 mgd from East-




land Woolen Mills and 0.2 mgd from the town of Corinna.  The town of




Dexter upstream of Corinna on the East Branch of the Sebasticook River




does not have a waste treatment facility and domestic and industrial




wastes are discharged directly to the river.  Being considered are two




alternatives for waste water treatment for Dexter, either construction




of a new WWTP or a tie-in with the plant in Corinna, after modification.

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     With the elimination of one major source of phosphorus pollution




and the implementation and operation of waste treatment facilities,  a




technical study was undertaken by the Region I Division of Surveillance




and Analysis, Biology Section to make an up-to-date assessment of Sebas-




ticook to determine if the lake is demonstrating signs of recovery from




the advanced eutrophic state reported in 1965.  The program was initiated




in November 1971 (autumn) and continued on a quarterly basis for the




winter and spring quarters of 1972 and the spring and summer quarters of




1973.






Conduct of Study




     Sample station locations Figure 1 and 2 are the sampling locations




used for the 1971-1973 study.  These stations on Lake Sebasticook and




on the East Branch of the Sebasticook River are contiguous with stations




designated in 1965 by Mackenthun.  In the summer of 1973 Lake Wassookeag




was sampled as a control and samples of substrate were obtained upstream




of the mouth of the East Branch Sebasticook River to determine the




nutrient potential in the river bottom.  The 1965 reference stations are




listed below those symbols used in 1971-1973, Figures 1 and 2.  Table 1




indicates the approximate station locations in terms of longitude and




latitude.






Sampling Procedures




     Water samples were collected from boats, bridges or through drilled




ice holes using a Kemmerer water sampler.  The lake samples were collected




at three depths and the tributary samples were collected from one depth.




Benthic sediment samples for biological and chemical analysis were collected




using a Petersen grab sampler.  All samples were preserved and analyzed,




according to standard procedures. C3»4>~5)




                                   -5-

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   MULLIGAN
    STREAM
EAST BRANCH
  SEBASTICOOK RIVER
                                                                                       STETSON STREAM.
         1/2
        MILES


    AREA: 4,288 ACRES



 6 SAMPLE STATION



00 I DEPTH CONTOURS IN
                                                                            LAKE  SEBASTICOOK

                                                                          SAMPLING STATION  LOCATIONS
                                                                                  1965 AND 1973

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         LWOI
     LAKE
   WASSOOKCAG
LW03
LW02
                      \,\\co
                 MOOS£HF.
                 MILL POND
              LAKE  SEBASTICOOK TRIBUTARIES
                  SAMPLING STATION  LOCATIONS
                         1965 AND 1973
                         -7-
                                                      flOUftl *

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                              BIOLOGY




Benthic invertebrates




     Bottom muds were sampled in 1971, 1972 and 1973 (Table 1)  (Figure 1)




to make a comparison of benthic animals with the 1965 survey findings.




Fine, soft, black mud at Stations LS01 through LS05 (Tables 2 and 3)  had




sparse populations of midgefly larvae (Chironomus riparius) and sludge-




worms (Tubificidae).  An average of 35 organisms per square foot, 57%




midgefly larvae and 43% sludgeworms, were found during the May, 1965




survey.   Similar benthic populations existed six years later (Nov.  1971)




with an average of 44 organisms per square foot, 25% midgefly larvae and




72% sludgeworms, and May 1972 with an average of 14 organisms per square




foot, 54% midgefly larvae and 46% sludgeworms (Table 6).   Special charac-




teristics of the blood of both animals facilitate extraction of dissolved




oxygen from oxygen deficient environments.  The midgefly, Chironomus rip-




arius , has two pairs of finger-like anal gills for greater aid in the




respiratory process.





     Three stations were selected in May, 1972 (LS06, LS07, LS08) near




river and stream inlets' for benthic invertebrates analysis of shallow




areas (average depth 6 feet compared to 40 feet for Stations LS01 through




LS05).




     Soft mud at Station LS06 near the mouth of Mulligan  Stream supported




a variety of 6 kinds of life, pollution tolerant sludgeworms dominated




the benthic life on this polluted substrate (Table 3).  LS06 is in the




general area of the inlet of the E.B. Sebasticook River.




     Water of apparent poor quality flows over the hard,  gravel and sand




substrate at Station LS07 near the mouth of the East Branch of the
                                -8-

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Sebasticook River as it supported a scant population of only 18 animals




per square foot and was lacking the clean water mayflies and caddisflies.







     Living on the gravel and sand bottom of Station LS08 leading into




Stetson Arm and Stetson Stream were a great variety of benthic fauna,




14 kinds, and greater numbers, 280 bottom organisms per square foot,




including pollution sensitive forms such as caddisflies, mayflies,




planarians, hydrozoans, ostracods and cladocerans.






     Organic nutrients have not accumulated on this hard substrate as




flow from Pleasant Lake by route of Stetson Stream contributes water of




good quality which enables a diverse population of benthic animals to




survive.  The bottom fauna on the natural substrate of Stetson Arm




(Station LS08) contributes a food source that would support a sport




fishery and is the only area of the lake where fishermen were observed




during the May, 1972 survey.  In contrast, environmental degradation of




bottom sediments caused by excessive build-up of organic nutrients at




Stations LS01-LS06 and the poor water quality at Station LS07 is




indicated by the presence of pollution tolerant benthos and their paucity




of numbers.






     The hard substrate of Station LS07 (50% gravel, 50% sand)  with




7 feet of water is similar to the hard substrate of Station LS08 (50%




gravel, 50% sand)  with six feet of water.   A slow water current flows




over these stations.






     Benthos was sampled in 1973 (Stations LS01-LS05)  with the average




number of organisms for the month of May being 18 per sq.  ft.  and July




being 3 per square foot.   Two control stations in Lake Wassookeag




                                  -9-

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 (LW02 and LW03) in August 1973 yielded 137 organisms per square foot




 (Tables 4, 5 and 6).







     Strong hydrogen sulphide odors were detected in bottom sediments




and a depleted oxygen level of 0.6 parts per million in the lower depths




of Lake Sebasticook during the July-August 1973 survey which indicates




active biological decomposition and an environment that is close to




being saprobic thereby eliminating most aquatic animal life.







     A total of 17 pollution tolerant sludgeworms and midgefly larvae




 (Chironomus riparius) were collected in July from five stations in Lake




Sebasticook (Stations LS01-LS05)  which averaged 33 feet in depth, in




comparison, one control station in oligotrophic Lake Wassookeag  (LW03)




supported 103 organisms consisting of sludgeworms and clean water midge-




flies (Anatopynia sp.) in the profundal zone.  Mayflies and an alderfly




were found at a depth of approximately 30 feet.






     The soft mud substrate of control station LW02 (Aug. 1973) at a




water depth of 7 feet supported a variety of 13 clean water forms of




life.  A comparable station in Lake Sebasticook (LS06) with a soft mud




bottom at a water depth of 6 feet (May 1972)  had 6 kinds of life dominated




by pollution tolerant sludgeworms (Table 3 and 5).






Algae




     Increased availability of phosphorus resulted in algal blooms causing




the "green paint" covering the rock along the shoreline and the "pig-pen"




odors reported in the 1966 report.     Excessive algal growths have con-




tinued into the 1971-1973 survey periods and the rock along the shoreline

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were observed to have a dark green color; however,  odors of decaying




algae were not noticed.






     The seasonal averages for algal counts for all Lake Sebasticook




stations at the five foot, 15-30 foot and 30-55 foot depths for three




surveys in 1965 and for four surveys from February 1972 to July 1973




are listed in Table 7 for comparison purposes.  Averages were determined




at the five foot depth for all surveys rather than surface counts due




to piling up of surface algae from wind and wave action.  The effect of




piling is considered significant but not representative of uniform lake




conditions in this case.






     Climatological variation during the year, i.e., temperature, light




intensity, cloud cover, and wind can cause variation in algae 'populations




for different months at different vertical depths;  however, when compared,




algae counts for the spring and summer surveys at all depths in 1965 of




11,300 algae per milliter are similar to the spring and summer averages




for 1973 of 11,700 algae per milliter.






     To contrast again, the Lake Wassookeag control has a sparse phyto-




plankton population of 467 algae per milliter for the 1973 summer survey.




This reflects upon the eutrophism of Lake Sebasticook which is attributed




to the high phosphorus concentrations noted in the section on nutrients.






     High algal counts in the East Branch of the Sebasticook River are




noted downstream of Dexter at Station EB01 (8,500 per milliter) and below




Corinna at Station EB02 (20,600 per milliter).  A substantially higher




average was recorded in 1965 for Station EB02 (Station 5 in 1965) of
                                   -11-

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99,000 per milliter (Table 8).  This massive algal growth could have




resulted from the surface matting and subsequent shoreline decay of




algae observed during the 1965 survey of Lake Sebasticook, but was not




evidenced during the 1971-1973 surveys.







Chlorophyll a_




     In general, chlorophyll present in a lake increases as the lake




becomes more eutrophic and supports greater algal populations.   In Table




9, the averaged chlorophyll a^  values exhibit considerable variation at




different depths, from season to season during a year, and from season




to season for different years, due probably to thermal stratification,




temperature, light intensity, availability of nutrients, etc.;  but the




seasonal and yearly averages for all depths and stations show a similar




chlorophyll a.  average value during 1965 as in the period from 1971 to




1973.






     The chlorophyll a_  averages, from the upper, middle and lower depths




(0-5', 10-25', 30-55') from all stations for 1965 (summer and fall), of




11.63 ug/1 are similar to the summer (1973) and fall  (1971) chlorophyll a_




average of 11.68 ug/1 in Lake Sebasticook.  Overall, the average lake




chlorophyll a  concentration of 11.63 ug/1 is similar to the average Ch a_




concentration (11.26 ug/1) recorded for the 1971 to 1973 period.  From




this it is deduced that the relative amount of algal standing crop in




Lake Sebasticook shows no change in the period since 1965.






     The unfertilized waters of Lake Wassookeag, Lake Sebasticook1s




principle headwater source, has a summer 1973 chlorophyll a_  average




value of 0.38 ug/1.
                                    -12-

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                               NUTRIENTS







     Chemical parameters which are commonly associated with eutrophication




were sampled for five seasons; November 1971, February 1972, May 1972,




1973 and July 1973.  The results of the analysis for the samples are




listed in the Appendix.







     Two parameters, total phosphorus (TP) and total Kjeldahl nitrogen




(TKN) are discussed here as a means for comparison with data generated




in 1965.  Both Lake Sebasticook and the East Branch Sebasticook River are




assessed in this manner to determine changes and make evaluations relative




to eutrophication.  The TKN values as reported for 1965 are the sum of the




extrapolated organic nitrogen and ammonia (TKN=Org N+NH3).







Summary (Nitrogen and Phosphorus)




     The histograms (Figures 3, 4, 5) summarize the data for TKN and TP




for the fall, winter, spring and summer seasons.  Total phosphorus in




Lake Sebasticook for the fall of 1971 is approximately 2.5 times greater




than the comparable season in 1965.  In the winter of 1972 TP is 1.5




times greater than in 1965.  For the spring of 1973 the total phosphorus




is approximately 1.5 times greater than.those values reported for 1972




and 1965 with the 1972 and 1965 values being approximately equal.  The




1973 summer values for total phosphorus again exceed those values for




1965 by approximately 1.5 times.






     Total phosphorus for the East Branch of the Sebasticook River




(Figure 4) shows some variability from season to season and station to




station.  Station EB-01, (above Corinna) has higher phosphorus values
                                   -13-

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recently  (i. e., 1971-1973) than in 1965 with the summer of 1973




being the exception.  Station EB02, below corinna, demonstrates higher




values in 1965 than in 1971, 1972 or 1973 with the winter of 1972




being a major exception.  EB03, at the outlet of Lake Sebasticook,




exhibits total phosphorus concentrations which are higher overall for




1971-1973 than in 1965.  This reflects the higher TP concentrations




noted in Lake Sebasticook in Figure 3.






     Total Kjeldahl nitrogen, Figures 3 and 5, are compared in the same




fashion as total phosphorus.  For Lake Sebasticook for all seasons




from 1971 to 1973 the TKN values range from approximately two to




eighteen times less than in 1965.  Similarly, for the East Branch of the




Sebasticook TKN values are two to ten times less in 1971, 1972, 1973




than in 1965, the only exception being the winter 1972 value for Station




EB01 where the TKN's are only slightly higher in 1965.






     Overall, the average total Kjeldahl nitrogen values for both Lake




Sebasticook and the East Branch of the Sebasticook River are approximately




five times less for the five seasons from 1971-1973 compared to 1965.






     Values for other parameters in the nitrogen series, ammonia  (NH ),




nitrate (N03) and nitrite  (N02) appear in the Appendix.  These nitrogen




species are difficult to interpret because the concentrations contin-




ually change due to the many factors regulating the complex nitrogen




cycle in nature.  For this reason, a comparative analysis has not been




made, however, a general assessment of these parameters show a trend




similar to that for TKN but it is not as marked.
                                    -14-

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                                                4.6
                                                                   1965
FALL     WINTER     SPRING    * SUMMER             FALL     WINTER     SPUING



   TOTAL PHOSPHORUS (mg/l)                  TOTAL KJELDAHL NITROGEN (mg/\)



        SEASONAL AVERAGES-LAKE SEBASTICOOK, MAINE

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                 .34
    co
    D
    it
    O
    X
    •1
    n
    o
    a.  .1?
o
                                                                                         1965
            EBOI    EB02   EB03

           	  FALL 	
EBOI   EB02   EB03

	  WINTER 	
EBOI
 EBO2

SPRING
EBOS
EBOI   EB02   EBOS

	 SUIHIER 	
          EAST BRANCH SCBASTICOOK RIVER - SEASONAL  AVERAGE  TOTAL  PHOSPHORUS

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          IO.8i
4.3
                     5.9.
2.8
                                                                                 1965
     EBOI   EB02   EB03  j  EBOI   EB02   EB03
     	 FALL 	'	 WINTER 	
              EBOI
 EB02
SPRING
EB03
EBOI   EB02   EB03
	 SUMMER 	
  EAST BRANCH SEBASTICOOK  RIVER - SEASONAL A/ERAGE TOTAL KJELDAHL NITROGEN

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Sediments




     Sediment samples were collected from five lake stations (Figure 1)




for four seasons from November 1971 to July 1973.  The sediments were




analyzed for total Kjeldahl nitrogen and total phosphorus.  The results




of these analyses are presented as percent dry weight in Table 11.  For




comparative purposes the sediment data for the same parameters for




May 1965 is also included.






     Total phosphorus (TP) values for the sediments show some variability




from season to season.  The data for May 1972 compares favorably with




the data for May of 1965 in terms of lake average .100 vs. .106% dry




weight respectively, however, when the data for May 1973 is compared to




1965 and 1972 the data shows approximately a 25% decrease.  The data for




November 1971 and July 1973 also represent 20-25% TP reductions when




compared to May 1972 and 1965.  These differences can be partially ex-




plained by seasonal turnover during which time a recycling and restocking




of nutrient deficient lake water normally occurs with resultant decreases




in sediment concentrations.  The reduction of TP in the sediments as




shown in the data strongly suggests this phenomena to be a source of the




increased total phosphorus concentrations noted for the overlying waters




of Lake Sebasticook.






     Lake Sebasticook sediments data for nitrogen shows a marked decrease




in concentration from November 1971 to July 1973 when compared to the




1965 data.  Average values as shown in Table 11 represent approximately




a decrease of five times overall.
                                  -18-

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Nitrogen/Phosphorus Ratios



     Table 10 lists Nitrogen:  Phosphorus ratios for November 1971,



May 1972 and 1973 and for July 1973.  Table 10 also lists the May 1965



values.  The ratios show a substantial decrease for November 1971 -



July 1973 when compared to May 1965.  The literature does not offer a



definitive explanation as to the significance of high or low N:P ratios.


                      tfi 7 R 9)
Data in the literaturel ' ' '  for which phosphorus and nitrogen sediment



values are available show trends that suggest that low N:P ratios are



more commonly found in eutrophic lake systems and higher ratios are



more indicative of less fertile systems.  It appears that the high TP



concentrations found in eutrophic sediments are a factor in the low N:P



ratios.  The extremely low N:P ratios found for Lake Sebasticook in



1971 and 1972-1973 appear to be a result of the nitrogen values being



lower by a factor of approximately five.  TP in comparison is approxi-



mately the same or slightly less than in 1965.  Whether or not these



ratios indicate that Sebasticook is more eutrophic now than in 1965



cannot be determined unless the lower nitrogen can be explained.  Lake



Wassookeag, a noneutrophic lake at the headwaters of the East Branch



Sebasticook River has a N:P ratio of 7.5 for July 1973.





Discussion (Nutrients)



     The report entitled "Fertilization and Algae in Lake Sebasticook,



Maine," serves as baseline information which describes Lake Sebasticook



as very eutrophic and which also describes the East Branch of the



Sebasticook River as the principle source of nutrification to the lake.



By use of this data and information generated in 1965 a comparison is



made which correlates the condition of the 1960's with those conditions
                                    -19-

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as of July and August 1973.







     Prior to the start of our studies in November 1971, the major source




of phosphorus, a potato processing plant, to the river and thus to Lake




Sebasticook burnt down.  This quirk of fate together with the start of




secondary treatment for the'Town of Corinna has not reflected any




significant improvement in stream conditions or in the eutrophic lake




conditions reported in 1966, particularly with respect to phosphorus.







     Above Corinna at Station EB01, the five season average for total




phosphorus for 1971-1973 of 0.14 mg/1 is approximately 30% greater than




the average value recorded in 1965 for a comparable time frame.  The




increase in phosphorus suggests greater and/or additional inputs to the




river upstream, probably from industrial and municipal sources in the




Dexter area or from a nonpoint discharge.






     Station EB02, at the mouth of the East Branch of the Sebasticook




River, (the inlet to Lake Sebasticook) the average total phosphorus value




for five seasons 1971-1973 is approximately the same as that for the four




seasons average in 1965.  EB02 is below the town of Corinna's municipal




discharge and below the location of the former potato processing plant.




Considering these averages compiled from seasonal data, the indication is




that the new secondary treatment plant at Corinna and the total elimination




of the major industrial source of phosphorus pollution (55% of all TP dis-




charged to the East Branch Sebasticook River from the Corinna area in




1965) has not resulted in a decrease in TP being contributed to Lake




Sebasticook.
                                   -20-

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The fact that no significant change has occurred suggests other causes




or sources of phosphorus.  Among these other possibilities are inefficient




waste treatment at Corinna, agricultural runoff and phosphorus in sludge




deposits between Corinna and Lake Sebasticook which resuspend with gas




bubbles from decomposition and which are scoured from the bottom during




periods of high flow.  Actual analysis of two sludge samples collected




from the stretch of river during August 1973, approximately 2.0 miles




upstream of the inlet to Sebasticook, Figure 2, yielded results of approxi-




mately .07% TP dry weight.  This is considered to be a relatively high




concentration.  The high TP coupled with the fact that sludge was seen




floating to the surface with gas bubbles and carried with the current




into Lake Sebasticook demonstrates that the stream bottom below Corinna is




a discrete source of phosphorus pollution to the lake.  Efficiency of the




WWTP at Corinna should be evaluated and the role of agricultural runoff




as a serious potential source of phosphorus to the system should also




receive study to further determine exact phosphorus sources.  In 1965, it




was estimated that less than 2% of the TP in Lake Sebasticook came from




agricultural drainage.  Today, with the tendency to increase arable land




and farm production phosphorus runoff could be substantially higher and




therefore represent a significant non-point source.






     In the report entitled "Fertilization and Algae in Lake Sebasticook,




Maine" 1966, a total phosphorus objective of .02 mg/1 for Lake Sebasticook




was proposed.  This objective is based on the phosphorus concentrations




found for samples collected from Lake Wassookeag, a lake at the heart of




the headwaters of the East Branch of the Sebasticook River.  In 1965 the
                                    -21-

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TP concentrations did not exceed 0.02 mg/1 and the lake did not demon-




strate the problems associated with eutrophication which characterized




Lake Sebasticook.  Furthermore, to attain the .02 mg/1 objective it was




suggested that an 80% reduction of TP would be required from all incoming




wastes, which means essentially the East Branch of the Sebasticook River.




It was estimated that ten years would be required to deplete the nutrient




rich Lake Sebasticook water with inflowing nutrient poor river water.




According to our data there are no gains in this direction.   In the eight




years elapsed since 1965, Lake Sebasticook demonstrates an overall average




phosphorus increase in the range of 30%.  For the East Branch of the




Sebasticook River, the burning of the snowflake Canning Co., and the




secondary treatment plant at Corinna should logically lower the amount of




phosphorus contributed to the river and thus to Lake Sebasticook.  This




apparently is not the case, as stream phosphorus loads are approximately




the same as in 1965 below Corinna at the inlet to Lake Sebasticook.






     It appears that .02 mg/1 TP for Lake Sebasticook may be an unrealistic




objective.  Preliminary results of the National Eutrophication Survey in-




dicate that about one-third of the 242 lakes studied probably will have




mean concentrations greater than .05 mg/1 phosphorus even- after advanced




treatment for phosphorus removal (assuming that a 50% reduction in TP is




accomplished).      Our data to date suggests placement of Sebasticook into




this category of lakes.  Perhaps the only means of achieving the .02 mg/1




phosphorus objective may be similar to that method observed by Edmundson




in his Lake Washington studies.      He observed that when phosphorus




concentrations were reduced by 72% from approximately .08 mg/1 to .02 mg/1,

-------
the lake reverted from a eutrophic state to a noneutrophic state.  This




was accomplished by total diversion of the inflow of sewage treatment




plant effluent.  Although the measures were drastic, the results were




positive.







     The lake bed of Sebasticook, taken alone, is a vast source of




phosphorus for fertilization and algal growth.  This is well demonstrated




during the spring seasons of May 1965, 1972 and 1973.  It can be seen that




for May 1965 and 1972 the average water column TP values are both .06 mg/1




and similarly the TP in the sediments are .106% dry weight and .100% dry




weight respectively.  However, in May 1973 the average TP in the water




column is .09 mg/1 or a 50% increase over the previous spring data.




Corresponding to this, the average sediment value for May 1973 is .075%




dry weight, or approximately a 25% decrease from that of the previous




spring data.  The sediment phosphorus reservoir as indicated appears to




be a major supplement to the enriched condition in Lake Sebasticook and




probably will be for many more years in the future.






     Contrary to the upward trend in total phosphorus in the Lake Sebasti-




cook system, the nitrogen in the water and sediments show a decrease which




is approximately five times less than in 1965.  It is difficult to




determine whether or not the nitrogen decrease in Lake Sebasticook is




entirely due to decreased input as exemplified by the lower tributary load




or whether a denitrification process is occurring within the lake.  It




could be a combination of these.factors.  Denitrification, although not




completely understood, is known to occur in eutrophic lakes in both sedi-




ments and in the water column.   '     The process is more likely to occur
                                   -23-

-------
in anaerobic conditions such as found in sediments and overlying waters




of the hypolimnion of a eutrophic lake during the summer stagnation.




General warming trends and pH shifts to alkaline are factors contribu-




tory to denitrification.






Vertical Profiles - Ch a_,  pH,, Temp., D.O.




     Figure 6 shows the curves for mean chlorophyll a_,  pH, temperature




and dissolved oxygen concentrations for mean lake depth.  The vertical




profile for temperature shows the lake to be stratified during late July




1973.  The mean temperature differential between surface waters and hypo-




limnial waters is approximately 10°C.  Corresponding to the thermocline




are distinct stratifications for Ch £,  pH and D.O.






     Algal standing crop in Lake Sebasticook expressed as chlorophyll a.




is highest in the lake epilimnion or euphotic zone.  The mean Ch a  con-




centration of approximately 25 ug/1 in the upper water column decreases




with depth to a mean of 3 ug/1 in the hypolimnial waters.






     In the euphotic zone of lakes, algae utilize  (carbon dioxide) CO2




in photosynthesis and produce oxygen (C>2).  Vigorous photosynthetic



activity can produce supersaturated dissolved oxygen concentrations in the




zone of activity in the epilimnion.  The vertical profile for oxygen in




Figure 6 shows supersaturated conditions approximately 180% of saturation




in the upper water column; with depth the D.O. is reduced to almost O close




to the bottom, creating nearly anaerobic conditions.






     As algae photosynthize in the water, free C0_ is used, calcium




bicarbonate is reduced by precipitation of calcium carbonate, and the pH



       (14)
rises. v     The pH profile in Figure 6 dramatizes this quite well with a



mean pH near 10 in the epilimnion and a neutral pH at the bottom.




                                 -24-

-------
     Taken collectively the profiles for dissolved oxygen,  pH,




temperature and chlorophyll ei  show a distinct congruency towards




describing Lake Sebasticook as eutrophic.






Recommendations




     l:  Evaluate treatment efficiency for the WWTP in Corinna  and




determine the total phosphorus contribution to the East Branch  of the




Sebasticook River from the Corinna area.






     2.  Determine significant point discharges of municipal and




industrial wastes from the Dexter area which presently have a bearing




on the phosphorus load in the East Branch Sebasticook River.






     3.  Closely evaluate the significance of non-point discharges




such as agricultural runoff and other bank sources and the impact they




have on Lake Sebasticook.






     4.  Enforcement of the State of Maine's water quality standards




which require phosphorus removal on all point discharges, together with




appropriate land management practices, are strongly urged in order to




return Lake Sebasticook to a viable resource.






     5.  The State of Maine should monitor lake conditions at least




once every year as a means of evaluating and assessing lake conditions




relative to eutrophlcation.
                                   -25-

-------
10
15
20
25
30
35
10
15
20
35
  10
      10   20    30   40
       Ch0 ug/l
15   20   25
 TEMP.  °C
30
                  8    12
                  pH
                                         16
20
                                 05    10   15
                                        DO mg/l
                   LAKE SEBASTICOOK
                    JULY 31,  AUG. I, 1973
                        MEAN
CHLOROPHYLL o - pH - TEMPERATURE - DISSOLVED OXYGEN
                          vs.
                   MEAN  WATER DEPTH
                   -26-
                                     FIGURE 6

-------
                               Bibliography
1.  Department of Health,  Education,  and Welfare,  FWPCA, January, 1966,
    "Fertilization and Algae in Lake  Sebasticook,  Maine," Technical
    Advisory and Investigations Activities,  Cincinnati, Ohio.

2.  Mackenthun, K. M. , Kemp, L. E., Stewart,  R. K., February 1968,
    Nutrients and Algae in Lake Sebasticook,  Maine.  Journal Water
    Pollution Control  Federation,  Vol.  40, No.  2,  Part  2.

3.  Environmental Protection Agency 1971,  "Methods For  Chemical Analysis
    of Water and Wastes,"  Water Quality Office, Analytical Quality
    Control Laboratory, Cincinnati, Ohio.

4.  American Public Health Association  1971,  Standard Methods, 13th
    Edition, AWWA, APHA, WPCF,  New York, N.  Y.

5.  U. S.  Environmental Protection Agency, 1973, "Biological Field and
    Laboratory Methods" EPA-670/4-73-001,  Environmental Monitoring Series,
    Office of Research and Development, Cincinnati, Ohio.

6.  Kemp,  A., Gray, C., Mudrochova, A., Nutrients  in Natural Waters,
    1972,  "Changes in  C, N, P,  and S  in the  Last 140 Years In Three Cores.
    From Lakes Ontario, Erie and Huron," John Wiley & Sons, N.Y.,
    pgs. 251-279.

7.  Gahler, A.R., Sanville, W.D.,  April 1971, "Lake Sediments - charac-
    terization of Lake Sediments and  Evaluation of Sediment-Water Nutrient
    Interchange Mechanisms in the Upper Klamath Lake System," Pacific
    Northwest Water Laboratory, Water Quality Office, U.S. EPA, Corvallis,
    Oregon, pgs. 26-32.

8.  Konrad, J.G., Keeney,  D.R., Chesters,  G., Chem., K.L. 1970,  "Nitrogen
    and Carbon Distribution in Sediment Cores of Selected Wisconsin
    Lakes." J. Water Pollution Control  Fed.  42,2096-7.

9.  Williams, J., Syers, J., Harris,  R., Armstrong, D., 1970, "Adsorption
    Desorption of Inorganic Phosphorus  by  Lake  Sediments in a O.lm Na Cl
    System."  Env. Sci. and Tech^ Vol 4, No.  6.519

10. U.S. Environmental Protection Agency,  1973, "Critique on Proposed
    Phosphorus Standards," Pacific Northwest Environmental Research Lab,
    Corvallis, Oregon  pg.  3.

11. Edmondson W. T., 1970, "Phosphorus, Nitrogen and Algae in Lake
    Washington after Diversion of-Sewage," Science 14,  690-1.
                                   -27-

-------
                             Bibliography
12.  Brezonik, P.  L.,  1972 "Nitrogen:   Sources  and Transformations  in
     Natural Waters"  in Nutrients in. Natural Waters,  John Wiley  & Sons
     N.  Y.  Pg. 26-31.

13.  Brezonik, P.L.,  Lee,  F.  G.,  "Denitrification as  a Nitrogen  Sink in
     Lake Mendota, Wis.",  Env.  Sci.  &  Tech.  Vol 2,  No.  2,  Pg.  120-5.

14.  Ruttner, F. 1963, Fundamentals  of Limnology, Third Ed., Univ.  of
     Toronto Press, Canada,  Pg.  70.
                                     -28-

-------
        TABLE I

Lake Sebasticook, Maine
   Sampling Stations
      1965 - 1973
1971 1973
Stations
LS-01
LS-02
LS-03
LS-04
LS-05
EB-01
EB-02
EB-03
LS-06
LS-07
LS-08
LS-09
LW-01
LW-02
LW-03
1965


Stations Longitude
A 69°
B 69°
C 69°
D 69°
E 69°
3 69°
5 69°
8 69°
69°
69°
69°
69°
69°
69°
69°
15'
13'
14'
14'
14'
15'
15'
16'
16'
15'
13'
15'
18'
20'
19'
25"
28"
06"
32"
35"
41"
01"
31"
08"
23"
11"
54"
34"
18"
37"




Latitude
44°
44°
44°
44°
44°
44°
44°
44°
44°
44°
44°
44°
45°
45°
45°
52'
53'
50'
51'
51'
55'
53'
50'
53'
52'
51'
52'
01'
01'
01'
12"
09
32
35
06
28
09
25
03
36
31
56
54
05
18
II
II
II
II
It
11
II
II
M
II
11
»

n
             -29-

-------
                 TABLE 2
Benthic Organisms - Lake Sebasticook, Maine

Organisms
Oligochaeta (aquatic worms)
Tubificidae (sludge worms)
Diptera (midgeflies)
Chironomus riparius (bloodworms)
UJ
o
i Hirudinea (leech)
Gastropoda (snails)
Pelecypoda (clams)
Copepoda (copepods)
Cladocera (water fleas)
Amphipoda (scuds)
Ostracoda (seed shrimp)
Hydracarina (water mites)
Hydro zoa (hydra)
Turbellaria (planarian)
May 31, 1972
Per Sq. Ft.
Stations
LS 01 LS 02 LS 03 LS 04 LS 05 LS 06 LS 07 LS 08
4 64
16 17 134
46 1 15
7 21 3 8
11 1
12 1 80
9 1 36
1 4 38
5 29
1
3
1 3
1
5

-------
                                                     TABLE 2 (Con't)
                                     Benthic  Organisms  - Lake Sebasticook,  Maine
                                                    May 31,  1972
                                                    Per Sq.  Ft.
                                                                          Stations
i
OJ
Organisms
Ephemeroptera (mayflies)
Trichoptera (caddisf lies)


LS 01 LS 02 LS 03 LS 04 LS 05 LS 06 LS 07 LS 08
1
3
Kinds 0 1 2 1 26 814
Total 0 7 37 3 25 203 18 280

-------
                                                       TABLE 3
                                      Benthic Organisms - Lake Sebasticook/ Maine
                                                     May 30, 1973
                                                     Per Sq. Ft.
                                                                       Stations
       Organisms
LS 01
LS 02
LS 03
LS 04
LS 05
10
NJ
I
       Oligochaeta (aquatic worms)
         Tubificidae (sludge worms)

       Diptera
         Chironomus riparius (bloodworms)
                                          Kinds
                                          Total
               19
                2

               28
             27


             32



              2

             59
               2

               4
              0

              0

-------
                                                      TABLE 4
                           Benthic Organisms - Lake Sebasticook and Lake Wassookeag, Maine
                                               July 31 - Aug. 1, 1973
                                                    Per Sq. Ft.
     Organisms
                                          LS 01
LS 02    LS 03
LS 04
LS 05
LW 02
LW 03
U>
LJ
Oligochaeta (aquatic worms)
  Tubificidae (sludge worms)
Diptera (midgeflies)
  Chironomus riparius
  Anatopynia sp.
  PolypedilumTophiodes
  Unidentified
Ephemeroptera (mayflies)
  Hexagenia bilineata
  Tricory thidae
Megaloptera (alderflies)
  Sialis sp.
                                                          5

                                                          3
                                     29
                                     47
                                     11

                                     19
                                      1
                                                56
                                                                                                       38
Trichoptera (caddisflies)
Psychomyia sp.
Pelecypoda (clams)
Cladocera (water fleas)
Copepoda ( copepods )
Amphipoda ( s c uds )
Ostracoda (seed shrimp)
Bryozoa (moss animalcules)
Kinds 021
Total 087
8
13
19
8
1
11
1*
1 1 13
1 1 172

4
103
     *Colonial Form

-------
                                                      TABLE 5
                                 Benthic Organisms - Lake Sebasticook,  Maine
                                                Nov. 17,  1971
                                                 Per Sq.  Ft.
                                                                        Stations
       Organisms
LS 01
LS 02
LS 03
LS 04
LS 05
I
OJ
       Oligochaeta (aquatic worms)
         Tubificidae (sludge worms)

       Diptera (midgeflies)
                         100
                                     44
Chironomus Riparius
Copepoda (copepod)


(bloodworms)

Kinds 1
Total 1
5
1
3
13
20
5
3
125
3

2
11
29

2
73

-------
                            TABLE 6
Midgeflies - Sludgeworms
Average Per Sq. Ft.
Year
1965*
1965*
1971
1972
1973
1973
Month
May
July
Nov
May
May
July
Lake Sebasticook,
1965-1973
Average
Per Sq. Ft.
35
57
44
14
18
3
Maine
Midgefly Larvae
% Scj. Ft.
57%
62%
25%
54%
45%
24%
Sludgeworms
% Sq. Ft.
43%
38%
72%
46%
55%
76%
*Fertilization and Algae in Lake Sebasticook,  Maine
 Technical Advisory and Investigations Activities,
 Robert A. Taft Sanitary Eng.  Center,  Cincinnati,  Ohio
 January, 1966
                                 -35-

-------
                               TABLE 7

                        Lake Sebasticook, Maine
                         Algae Count (Average)
                             Per Milliter
                              1965 - 1973
                                                  Middle
                               Lower
Lake Sebasticook
  May 11-27, 1965*
  July 28-Aug. 1, 1965*
  Nov. 2, 1965*
  Feb. 15-16, 1972
  May 31, 1972
  May 31, 1973
  July 31, 1973

Lake Wassookeag  (Control)
  Aug. 1, 1973
5 ft.
17,875
27,684
 4,090
 4,852
 6,940
16,527
14,969
   283
15-30 ft.
10,416
 4,467
11,727
 9,576
 6,554
15,817
 2,863
   723
Seasonal and yearly averages for comparable seasons
  Lake Sebasticook
    Spring - Summer 1965           22,780
    Spring - Summer 1973           15,748

    1965     11,354 per milliter
    1973     11,715 per milliter

  Lake Wassookeag (Control)
    1973 (Summer) 467 per milliter
                7,441
                9,340
 30-55 ft.
 6,959
   725
 3,850
13,369
 7,778
16,594
 3,520
   396
                3,842
               10,057
*Fertilization and Algae in Lake Sebasticook,  Maine
 Technical Advisory and Investigation Activities
 Robert A. Taft Sanitary Engineering Center, Cinn., Ohio
 January 1966
                                  -36-

-------
                              TABLE 8
                 East Branch Sebasticook River/ Maine
                             Algae Count
                       Per Milliter (Average)
                 1965, 1971-1973 (Comparable Stations)
Station                          3                   58

May 11-27, 1965*               3,537              39,512             30,123
July 26-31, 1965*                610 -            224,150              7,275
Nov. 2, 1965*                    -                33,400              2,700

                     Average   2,073              99,020             13,366
Station                         EB01                EB02               EB03

Nov. 17, 1971                    -                10,358            .   -
Feb. 15, 1972                  6,000              28,979              5,830
May 31, 1972                   7,075               6,395              9,735
May 31, 1973                  16,987              39,620             26,205
July 31, 1973     .             4,245              17,716              4,641

                     Average   8,576              20,613             11,602
*Fertilization and Algae in Lake Sebasticook, Maine
Technical Advisory and Investigation Activities
Robert A. Taft Sanitary Engineering Center, Cinn. Ohio
January 1966.
                                   -37-

-------
                               TABLE 9
                      Lake  Sebasticook, Maine
                            Chlorophyll a_
                   Micrograms Per Liter (Average)
                              Upper (0-5')    Middle (10-25')    Lower (30-55')

July 28-Aug. 1, 1965*             21.97             3.67              2.99
Oct. 30-Nov. 2, 1965*             13.20            14.38             13.61
Nov. 17, 1971                      8.67             7.40              8.90
Feb. 15, 1972                      8.95             9.12              8.73
May 31, 1972                       5.29             5.32              5.53
May 30, 1973                      21.34            16.14             18.57
July 31, 1973                     24.63            17.45              3.04
Aug. 1, 1973   (Lake Wassookeag)   <0.10             0.94            <0.10

Seasonal and Yearly Averages for Entire Lake

July 28-Aug. 1, 1965   <9.54 \    <11.63
Oct. 30-Nov. 2, 1965

Nov. 17, 1971           8.32)     11.68
July 31, 1973
May 30, 1973           18.68^     16.86
July 31, 1973

Feb. 15, 1972           8.93^      7.15
May 31, 1972
All surveys Nov. 17, 1971 - July 31, 1973  11.26
All surveys July 28, 1965 - July 31, 1973  11.37
Aug. 1, 1973 (Lake Wassookeag)             <0.38
*Fertilization and Algae in Lake Sebasticook, Maine
Technical Advisory and Investigation Activities
Robert A. Taft Sanitary Engineering Center, Cinn.,  Ohio
January 1966
                                     -38-

-------
                                        TABLE 10
                               Lake Sebasticook,  Maine
                                      Sediments
                                Ni trogen/Phosphorous
                                       Ratios
Station Reference Station May 65*
LS 01
LS 02
LS 03
LS 04
LS 05
L. Wassookeag
A
B
C
D
E

8.8
8.7
5.6
7.5
6.4
20.0
Nov. 71
2.7
1.8
1.5
1.2
2.1

May 72
1.1
1.8
1.7
1.2
1.5

May 73
1.9
2.1 ,
1.3
1.3
2.1

July 73
1.8
2.3
1.5
1.7
1.8
8.9
*Seasonal Average 2 Values.
                                           -39-

-------
                                    TABLE
                           Lake Sebasticook,  Maine
                                  Sediments
                           Phosphorus and Nitrogen
                                % Dry Weight
Phosphorus
1971 - 1973
1965
May 65
Nov. 71
May 72
May 73
July 7!
Station Stations
LS01
LS02
LS03
LS04
LS05
Average
A
B
C
D
E
Lake Wassookeag
East Branch Sebasticook R.
Lagoon Upstream of
Inlet to Lake

LS01
LS02
LS03
LS04
LS05
Average

A
B
C
D
E
Lake Wassookeag
East Branch Sebasticook
.095
.120
.070
.120
.125
.106
.05

.85
.90 •
.40
.90
,80
.77
1.0
.075
.074
.080
.101
.082
.082

Nitrogen
.204
.130
.118
.120
.174
.149

. 114 '
.079
.083
.125
.101
.100


.133
.148
.147
.148
.157
.146

.090
.063
.062
.088
.074
.075


.173
.129
.083
.116
.154
.131

.084
.055
.077
.084
.078
.076
.027
.073

.152
.125
.114
.143
.141
.135
0.24
0.41
R. Lagoon Upstream of
Inlet to Lake
                                      -40-

-------
APPENDIX
 -41-

-------
                    Lake Sebasticook,  Maine
                         Chlorophyll a_
                     Micrograms Per Liter
                        Nov.  17, 1971
                                                                  A-l
Station

LS01

LS02

LS03

LS04

LS05
             Average
Upper

 8.18

 6.75

 6.04

 5.74



 8.67
Middle

 9.22

 8.34

 5.27

 6.75

 7.47

 7.40
Lower

14.54

 6.75

12.95

 9.61

10.65

 8.90
 EB01

 EB02

 EB03
  4.45

 40.81

  4.46
                              -42-

-------
                                              A-2
Lake Sebasticook, Maine
     Chlorophyll £
Micrograms Per Liter
   Feb. 15-17, 1972
Station

LS01
LS02
LS03
LS04
LS05
Average
EB01
EB02
EB03
Upper
5'
7.08
15.18
6.92
10.25
5.34
8.95
10.61
8.21
4.61
Middle
10-25'
11.84
4.61
11.13
10.25
7.80
9.12



Lower
30-55'
4.62
5.85
26.11
2.32
4.77
8.73



             -43-

-------
                                              A-3
Lake Sebasticook, Maine
     Chlorophyll a,
 Micrograms Per Liter
     May 31, 1972
Station

LS01
LS02
LS03
LS04
LS05
LS06
LS07
LS08
Average
EB01
EB02
EB03
Upper
5'
6.11
5.41
5.98
6.55
A. 26
6.11
5.53
2.41
5.29
3.12
10.13
8.07
Middle Lower
10-25' 30-55'
4.26 5.41
5.53 7.50
6.49 9.22
6.10 4.39
4.26 4.26
-
3.12
4.83
5.32 5.53



           -44-

-------
                                                                      A-4
                       Lake Sebasticook,  Maine
                            Chlorophyll a_
                    Micrograms Per Liter of Water
                    July 31, 1973 - August 1, 1973
Station
Upper Middle
0-5' 10-25'
LS
LS
LS
LS
LS
LS
01
02
03
04
05
09
11
7
3
2
13
108
.91 2.55
.81 5.37
.86 19.33
.63 52.11
.37 7.88
.25
Lower
30-55'
0.39
0.01
11.69
2.65
0.47
-
            Average          24.63          17.45            3.04
EB 01                         6.10

EB 02                        36.54

EB 03                         4.79

LS 01 (Lake Wassookeag)     <0.10           0.94          < 0.10
                                  -45-

-------
                                                                   A-5
                    Lake Sebasticook,  Maine
                         Chlorophyll a_
                     Micrograms Per Liter
                         May 31, 1973
Station                       Upper          Middle          Lower

 LS01                         29.07           15.31          17.39

 LS02                         22.34           22.95          16.92

 LS03                         19.32           16.92          25.52

 LS04                         17.31           18.24          18.21

 LS05                         18.70            7.31          14.65

             Average          21.34           16.14          18.57



 EB01                          5.18

 EB02                          7.00

 EB03                         21.62
                                  -46-

-------
                                                               A-6
                   Lake Sebasticook, Maine
                        Algae Count
                       Nov.  17,  1971
                       Per Milliter
Station                        Upper

 LS01                           906

 LS02                         1,075

 LS03                           962

 LS04                           792

 LS05                           912


              Average           929


 EB01

 EB02                        10,358

 EB03
                              -47-

-------
                       Lake Sebasticook, Maine
                            Algae Count
                          Feb. 15-16, 1972
                           Per Milliter
                                                                     A-7
Station

LS01

LS02

LS03

LS04

LS05
 Upper

 3,000

 3,396



 4,302

 8,716
 Middle

 6,735

22,300

 8,000

 9,735

 3,113
 Lower

14,716

 9,339

23,376

 9,622

 9,792
              Average
EB01

EB02

EB03
 4,852


 6,000

28,979

 5,830
 9,576
13,369
                                  -48-

-------
                                                                   A-8
Station




 LS01




 LS02




 tS03




 LS04




 LS05




 LS06




 LS07




 LS08
 EB01




 EB02




 EB03
                Average
Lake Sebasticook, Maine
Algae Count
May 31,1972
Per Milliter
Upper Middle
3,283 8,150
7,415 3,622
7,811 8,320
13,301 6,113
8,830 6,566
6,905
4,358
3,622
ge 6,940 6,554
7,075
6,395
9,735

Lower
6,622
6,452
8,547
13,641
8,999
-
6 , 169
4,019
7,778



                                 -49-

-------
                                                                    A-9
Station

 LS01

 LS02

 LS03

 LS04

 LS05
 EB01

 EB02

 EB03
                      Lake Sebasticook,  Maine
                            Algae Count
                            May 31,1973
                            Per Milliter
                Upper              Middle

                16,357             16,659

                19,470             21,111

                23,376             14,829

                14,150             18,282

                 9,282              8,207


Average         16,527             15,817


                16,987

                39,620

                26,205
Lower

11,829

22,017

25,696

13,018

10,414


16,594
                                -50-

-------
                                                                   A-10
                    Lake Sebasticook, Maine
                          Algae Count
                          Per Milliter
                   July 31 and Aug. 1, 1973
Station
LS01
LS02
LS03
LS04
LS05
LS09
Average
EB01
EB02
EB03
Upper
2,264
1,585
1,302
2,660
2,207
79,800
14,969
4,245
17,716
4,641
Middle
283
2,038
3,339
4,471
4,188
-
2,863



Lower
1,698
1,868
4,641
2,434
6,962
-
3,520



LW01 (Lake Wassookeag)              283                723            396
                              -51-

-------
                                                                    A-ll
                      Lake Sebasticook,  Maine
                   Total Kjeldahl Nitrogen as  N
                          Feb.  17, 1972
                              MG/L
Station

 LS01

 LS02

 LS03

 LS04

 LS05

                Average


 EB01

 EB02

 EB03
Upper
0.7
0.2
0.4
0.3
0.4
0.4
1.8
0.7
0.2
Middle
0.2
0.3
0.3
0.3
0.4
0.3



Lower
0.5
0.3
1.2
0.5
0.3
0.6



                                 -52-

-------
                   Lake Sebasticook, Maine
                Total Kjeldahl Nitrogen as N
                            MG/L
                                                                A-12
                        June  1,  1972
Station

 LS01

 LS02

 LS03

 LS04

 LS05
 EB03

 EB02

 EB01
 LS01

 LS02

 LS03

 LS04

 LS05
           Average
           Average
Upper
0.9
0.2
0.7
1.0
0.8
0.7
0.6
1.9
0.2
Nov. 17, 1971
0.8
0.4
0.3
0.4
0.4
0.5
Middle
0.6
0.8
0.7
0.8
0.6
0.7




0.8
0.4
0.3
0.3
0.6
0.5
                                     Lower

                                      0.8

                                      0.9

                                      0.7

                                      0.7.

                                      0.9

                                      0.8
                                       0.4

                                       0.5

                                       0.5

                                       0.3

                                       0.3

                                       0.4
 EB01

 EB02

 EB03
0.5

2.5

0.2
                             -53-

-------
                                               A-13
Lake Sebasticook, Maine
   Ammonia (NH_ - N)
         MG/L
                    May 31, 1972
Station
LS01
LS02
LS03
LS04
LS05

EB01
EB02
EB03

LS01
LS02
LS03
LS04
LS05

EB01
EB02
EB03
Upper
.01
.01
.01
.05
.01
Average 0.02
.01
.83
.05

.37
.22
.07
.11
.03
Average • 16
1.48
.75
.07
Middle Lower
.01 .12
.04 .52
.19 -20
.04 .03
.19 	
0.09 0.22



Feb. 15, 1972
.10 5.36
.27 1.62
.37 .06
.03. -42
.03 .33
.16 1-56



            -54-

-------
                                                                    A-14
                    Lake Sebasticook, Maine
                       Total Phosphorus
                             MG/L
                         May 31, 1973
Station

 LS01

 LS02

 LS03

 LS04

 LS05

               Average


TOTAL PHOSPHORUS CONCENTRATION = 0.094 MG/L


 EB01                                .23

 EB02                                .06

 EB03                                .05
Upper
.21
.06
.06
.04
.05
.084
Middle
.05
.04
.07
.04
.07
.054
Lower
.28
.13
.08
.16
.07
.144
                              -55-

-------
                                                                   A-15
Station




 LS01




 LS02




 LS03




 LS04




 LS05
 EB01





 EB02




 EB03










 LS01




 LS02




 LS03




 LS04




 LS05
  EB01





  EB02





  EB03
Lake Sebasticook, Maine
Nitrate - Nitrite
MG/L
May
Upper
.01
.01
.01
.02
.02
ige .01
.01
.04
.04
Feb
.01
.03
.06
.02
.08
age . 04

31, 1972
Middle
.01
.07
.06
.06
.03
.05



. 15-16, 1972
.06
.07
.02
.03
.03
.04


Lower
.12
.07
.14
.02
.02
.07




.04
.02
.07
.15
.08
.07
 .21




 .26




1.45
                                -56-

-------
                      Lake Sebasticook, Maine
                          Nitrite, N02~N
                              MGA
                          May  31,  1973
                                                                    A-16
Station


 LS01


 LS02

 LS03

 LS04

 LS05
              Average
Upper

 0.01

 0.01

 0.01

 0.01

 0.01

 0.01
Middle


 0.01

 0.01

 0.01

 0.01

 0.01

 0.01
Lower


 0.01

 0.01

 0.01

 0.01

 0.01

 0.01
AVERAGE NITRITE  0.01
 EB01


 EB02


 EB03
 0.01


 0.01


 0.01
                                -57-

-------
                      Lake Sebasticook, Maine
                           Nitrate  NO..-N
                               MG/L
                           May 31,  1973
                                                                    A-17
Station

 LS01

 LS02

 LS03

 LS04

 LS05
               Average
Upper

 0.05

 0.05

 0.05

 0.05

 0.05

 0.05
Middle

 0.19

 0.05

 0.05

 0.05

 0.05

 0.08
Lower

 0.08

 0.05

 0.37

 0.05

 0.05

 0.12
TOTAL NO -N CONCENTRATION =0.08
 EB01

 EB02

 EB03
 0.05

 0.05

 0.13
                              -58-

-------
                    Lake Sebasticook, Maine
                         Ammonia NH3-N*
                              MG/L
                          May 31, 1973
                                                                   A-18
Station

LS 01

LS 02

LS 03

LS 04

LS 05
                Average
Upper

0.20

0.20

0.20

0.20

0.20

0.20
Middle

 0.20

 0.20

 0.20

 0.20

 0.20

 0.20
Lower

0.20

0.20

0.20

0.20

0.20

0.20
Total NH.,-N Concentration = 0.20
EB 01                           0.20

EB 02                           0.20

EB 03                           0.20



*A11 Values Less Than Recorded
                                 -59-

-------
                                                                   A-19
S tatioji

LS 01

LS 02

LS 03

LS 04

LS 05
                   Lake Sebasticodk,  Maine
                   Total Kjeldahl Nitrogen
                             MG/L
                         Aug. 1,  1973
Upper

 0.67

 1.60

 0.27

 0.93

 0.54
              Average
 0.80
Middle

•0.67

 0.70

 0.61

 0.21

 0.26

 0.49
Lower

0.40

0.34

0.44

0.42

0.54

0.43
Average TKN = 0.57
EB 01

EB 02

EB 03
 0.35

 0.85

 0.38
                               -60-

-------
                                                               A-20
                 Lake Sebasticook, Maine
                       Algae Count
                       Nov.17,  1971
                       Per Milliter
Station                      Upper

 LS01                         906

 LS02                        1,075

 LS03                         962

 LS04                         792

 LS05                         912

           Average            929


 EB01

 EB02                       10,358

 EB03
                             -61-

-------
                                                                   A-21
Station




 LS01




 LS02




 LS03




 LS04




 LS05




 LS06




 LS07




 LS08
 EB01




 EB02




 EB03
            Average
Lake Sebasticook, Maine
Algae Count
May 31, 1972
Per Milliter
Upper
3,283
7,415
7,811
13,301
8,830
6,905
4,358
3,622
6,940
7,075
6,395
9,735

Middle
8,150
3,622
8,320
6,113
6,566
-
-
_
6,554



 Lower




 6,622




 6,452




 8,547




13,641




 8,999









 6,169




 4,019




 7,778
                                 -62-

-------
                     Lake Sebasticook,  Maine
                           Algae Count
                        Feb.  15-16,  1972
                           Per Milliter
                                                                    A-22
Station

 LS01

 LS02

 LS03

 LS04

 LS05
              Average
 EB01

 EB02

 EB03
Upper

 3,000

 3,396



 4,302

 8,716

 4,852


 6,000

28,979

 5,830
Middle

 6,735

22,300

 8,000

 9,735

 3,113

 9,576
Lower

14,716

 9,339

23,376

 9,622

 9,792

13,369
                                -63-

-------
                   Lake Sebasticook,  Maine
                         Algae Count
                        May 31, 1973
                        Per Milliter
                                                                   A-23
Station

 LS01

 LS02

 LS03

 LS04

 LS05
              Average
 EB01

 EB02

 EB03
Upper

16,357

19,470

23,376

14,150

 9,282

16,527


16,987

39,620

26,205
Middle

16,659

21,111

14,829

18,282

 8,207

15,817
Lower

11,829

22,017

25,696

13,018

10,414

16,594
                                 -64-

-------
                                                                      A-24
                     Lake Sebasticook,  Maine
                           Algae Count
                           Per Milliter
                     July 31 and Aug. 1,  1973
Upper
2,264
1,585
1,302
2,660
2,207
79,800
14,969
4,245
17,716
4,641
Middle
283
2,038
3,339
4,471
4,188
_
2,863



Lower
1,698
1,868
4,641
2,434
6,962
_
3,520



S tation

LS 01

LS 02

LS 03

LS 04

LS 05

LS 09

               Average


EB 01

EB 02

EB 03
LW 01   (Lake Wassookeag)         283            723             396
                                   -65-

-------
                                                 A-25
Lake Sebasticook, Mai,ne
       Nutrients
  7/31, 8/1, 8/2, 1973
Station #
LS01S
LS01M
LS01B
LS09S
LS02S
LS02M
LS02B
LS03S
LS03M
LS03B
LS04S
LS04M
LS04B
LS05S
LS05M
LS05B
EB01
EB02
EB03
Depth
2.0
18.0
35.0
2.0
2.0
21.0
42.0
2.0
11.0
22.0
2.0
18.0
32.0
2.0
17.0
32.0
2.0
2.0
2.0
TKN
0.2
0.2
1.4

0.3
0.5
2.0
0.3
0.2
1.2
0.4
0.2
1.5
0.4
0.3
0.8
0.3
2.1
0.3
NO^-N
K.02
K.02
K.02
K.02
.04
K.02
K.02
0.05
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
N03-N
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
K.02
HH3^N
0.02
0.07
0.34
.06
.02
.04
1.4
.02
.06
.01
.01
.03
1.4
.02
0.02
0.78
0.10
1.5
.06
TP
0.06
0.08
0.05
K.05
K.05
0.41
K.05
K.05
.05
K.05
K.05
0.38
K.05
K.05
0.22
0.14
.05
.05
0.15
             -66-

-------
                                                                   A-26
                   Lake Sebasticook, Maine
Station

 LS01
 LS02
 LS03
 LS04
 LS05
 EB01
 EB02
 EB03
 LS01
 LS02
 LS03
 LS04
 LS05
 EB01
 EB02
 EB03
 LS01
 LS02
 LS03
 LS04
 LS05
 EB01
 EB02
 EB03
          Average
          Average
          Average
Total Phosphorus
MG/L
Nov. 17, 1971
Upper
.10
.08
.06
.06
.20
.10
.14
.18
.06
Feb. 15-17, 1972
_
.04
.04
.06
.04
.04
.20
.50
.10
May 31, 1972
.04
.03
.03
.08
.02
.04
.11
.11
.57



Middle
.10
.10
.06
.06
.06
.08




.16
.06
.05
.34
.06
.13




.02
.06
.04
.07
.09
.06



Lower
                               -67-

-------