OFTS-TECHNICAL INFO-
&EPA
            Agency
Off ica of
Pesticides and Toxic Substances
Washington DC 20460
                                      August 1981
            Pesticides
             Naphthaleneacetic Acid its
             Salts, Ester, and Acetamide
             Pesticide Registration
             Standard


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             Naphthalcneacetic  /fcid
                its Salts, Ester,
                  and Azetamide

          Pesticide Registration Standard
Michael F. Branagan    Project Manager (SPRD)
William Airiia          Plant Sciences Specialist  (PSB/BFSD)
Gary Ballard           Economist (EAB/BFSD)
Hudson Boyd            Environmental Chemist  (EFB/HED)
Dan Dickson            Product Manager (FHB/RD)
Nancy Dodd             Residue Chemist (RCB/HED)
Linda Garczynski       Writer/Editor (SPRD)
Robert ftxLst           Plant Physiologist  (EEB/HED)
Thomas Johnston        Wildlife Ecologist  (EEB/HED)
Ellen Sullivan         Pharmacologist (TB/HED)

                  July 31, 1981

     Office of Pesticides and Toxic Substances

          Environmental Protection Agency

                 401 M Street, SW

             Washington, D.C.  20460

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                      - TABLE OP CONTENTS -

                                                                 page
Chapter I     How to Register under a Registration Standard	1


Chapter II    Regulatory Position and Rationale	9


Chapter III   Sunnary of Data Requirements and Data Gaps	19


Chapter IV    Product Chemistry	25


Chapter V     Environmental Pate...	27


Chapter VI    Toxicology	*	32


Chapter VII   Residue Chemistry.	43


Chapter VIII  Ecological Effects	45


Bibliography	47

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           CHAPTER I:  HOW TO REGISTER UMDER A REGISTRATION STANDARD

    A.  Organization of the Standard
    B.  Purpose of the  Standard
    C.  Requirement to  Re-register Under the Standard
    D.  "Product Specific" Data and "Generic* Data
    E.  Data Compensation Requirements under P1PRA 3(c) (1) (D)
    P.  Obtaining Data  to Pill " Data Gaps"; FIFRA 3(c) (2) (B)
    G.  Amendments to the Standard

A.  Organization of the Standard

This first chapter explains the  purpose of a Registration Standard and
summarizes the legal  principles  involved in registering or re-registering under
a Standard.   The second chapter  sets forth the requirements that must be met to
obtain or  retain registration for products covered by  this particular
Registration  Standard.   In the remaining chapters, the Agency reviews the
available  data by scientific discipline, discusses the Agency's concerns with
the identified potential hazards, and logically develops  the conditions and
requirements  that would reduce those hazards to acceptable levels.

B.  Purpose of the Standard

Section 3  of  the Federal Insecticide, Fungicide, and Rodenticide Act (PIFRA)
provides that "no person in any  State may distribute,  sell,  offer for sale,
hold for sale, ship,  deliver for shipment, or receive  (and having so received)
deliver or offer to deliver, to  any person any pesticide  which is not
registered with the Administrator [of EPA]."  To approve  the  registration of a
pesticide, the Administrator must find, pursuant to Section 3(c)(5)  that:

    "(A)  its  composition is such as to warrant the proposed claims for it;

    (B)  its  labeling and other  material required to be submitted comply
         with the requirements of this Act;

    (O  it will perform its intended function without unreasonable adverse
         effects on the environment; and

    (D)  when used in accordance with widespread and commonly recognized
         practice it  will not generally cause unreasonable adverse effects
         on the environment."

In making  these findings, the Agency reviews a wide range of data which
registrants are required to submit,  and assesses the risks and benefits
associated with the use of the proposed pesticide.  But the established
approach to making these findings has been found to be defective on two countsj

First, EPA and its predecessor agency, the United States  Department of
Agriculture (USDA), routinely reviewed registration applications on a "product
by product" basis,  evaluating each product-specific application somewhat
independently.   In the  review of products containing similar components, there

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 was little opportunity for a retrospective review of the full range of
 pertinent data available in Agency files and in the public literature. Thus the
 "product by product" approach was often inefficient and sometimes resulted in
 inconsistent or incomplete regulatory judgments.

 Second, over the years, as a result of inevitable and continuing advances in
 scientific knowledge, methodology, and policy, the data base for many
 pesticides came to be considered inadequate by current scientific and
 regulatory standards.  Given the long history of pesticide regulation in
 several agencies,  it is even likely that materials may have been lost from the
 data files.  When EPA issued new requirements for registration in 1975  (40 CFR
 162)  and proposed  new guidelines for hazard testing in 1978 (43 FR 29686, July
 10, 1978 and 43 FR 37336, August 22, 1978), many products that had already been
 registered for years were being sold and used without the same assurances of
 human and environmental safety as was being required for new products.  Because
 of this inconsistency, Congress directed EPA to re-register all previously
 registered products, so as to bring their registrations and their data bases
 into compliance with current requirements, [See FIFRA Section 3(g)].

 Facing the enormous job of re-reviewing and calling-in new data for the
 approximately 35,000 current registrations, and realizing the inefficiencies of
 the "product by product" approach, the Agency decided  that a new, more
 effective method of review was needed.

 A new review procedure has been developed.  Under it, EPA publishes documents
 called Registration Standards, each of which discusses a particular pesticide
 active ingredient.  Each Registration Standard summarizes all the data
 available to the Agency on a particular active ingredient and its current uses,
 and sets forth the Agency's comprehensive position on the conditions and
 requirements for registration of all existing and future products which contain
 that active ingredient.  These conditions and requirements, all of which must
 be met to obtain or retain full registration or reregistration under Section
 3(c)(5)  of FIFRA,  include the submission of needed scientific data which the
 Agency does not now have, compliance with standards of toxicity, composition,
 labeling, and packaging, and satisfaction of the data compensation provisions
 of FIFRA Section 3(c)(1)(D).

 The Standard will  also serve as a tool for product classification.  As part of
 the registration of a pesticide product, EPA may classify each product for
 "general  use"  or "restricted use" [FIFRA Section 3(d)].  A pesticide is
 classified  for "restricted use" when some special regulatory restriction is
 needed  to ensure against unreasonable adverse effects to man or the
 environment.   Many such risks of unreasonable adverse effects can be lessened
 if  expressly-designed  label  precautions are strictly followed. Thus the special
 regulatory  restriction for a "restricted use" pesticide is usually a
 requirement  that it be applied only by, or under the supervision of, an
applicator who has been certified by the State or Federal government as being
competent to  use pesticide safely, responsibly, and in accordance with label
directions.  A restricted-use pesticide can have other regulatory restrictions
 [40 CFR 162.11(c)(5)]  instead of, or in addition to, the certified applicator
requirement.   These  other regulatory restrictions may include such actions as
seasonal  or  regional limitations on use, or a requirement for the monitoring of

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residue levels after use. A pesticide classified for "general use," or not
classified at all, is available for use by any individual who is in compliance
with State or local regulations.  The Registration Standard review compares
information about potential adverse effects of specific uses of the pesticide
with risk criteria listed in 40 CFR 162.11(c), and thereby determines whether a
product needs to be classified for "restricted use."  If the Standard does
classify a pesticide for "restricted use," this determination is stated  in the
second chapter.

C. Requirement to Reregister Under the Standard

FIFRA Section 3(g), as amended in 1978, directs EPA to reregister all currently
registered products as expeditiously as possible.  Congress also agreed  that
reregistration should be accomplished by the use of Registration Standards.

Each registrant of a currently registered product to which this Standard
applies, and who wishes to continue to sell or distribute his product  in
commerce, must apply for reregistration.  His application must contain proposed
labeling that complies with this Standard.

EPA will issue a notice of intent to cancel the registration of any currently
registered product to which this Standard applies if the registrant fails to
comply with the procedures for reregistration set forth in the Guidance  Package
which accompanies this Standard.

D. "Product Specific" Data and "Generic" Data

In the course of developing this Standard, EPA has determined the types  of data
needed for evaluation of the properties and effects of products to  which the
Standard applies, in the disciplinary areas of Product Chemistry, Environmental
Fate, Toxicology, Residue Chemistry, and Ecological Effects.  These
determinations are based primarily on the data Guidelines proposed  in  43 FR
29696, July 10, 1978; 43 FR 37336, August 22, 1978; and 45 FR 72948, November
3, 1980, as applied to the use patterns of the products to which this  Standard
applies.  Where it appeared that data from a normally applicable Guidelines
requirement was actually unnecessary to evaluate these products, the Standard
indicates that the requirement has been waived.  On the other hand, in some
cases studies not required by the Guidelines may be needed because  of  the
particular composition or use pattern of products the Standard covers;  if so,
the Standard explains the Agency's reasoning.  Data guidelines have not  yet
been proposed for the Residue Chemistry discipline, but the  requirements for
such data have been in effect for some time and are, the Agency believes,
relatively familiar to registrants.  Data which we have found are needed to
evaluate the registrability of some products covered by the  Standard may not be
needed for the evaluation of other products, depending upon  the composition,
formulation type, and intended uses of the product in question.  The Standard
states which data requirements apply to which product categories.   (See  the
third chapter.)  The various kinds of data normally required for  registration
of a pesticide product can be divided into two basic groups:

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    1. Data that are product specific , i.e. data that relates only to  the
       the properties or effects of a product with a particular composition
       (or a group of products with closely similar composition); and

    2. Generic data that pertain to the properties or effects of a
       particular ingredient, and thus is relevant to an evaluation of
       the risks and benefits of all products containing that ingredient
       (or all such products having a certain use pattern), regardless of
       any such product's unique composition.

 The Agency requires certain "product specific" data for each product to
 characterize the product's particular composition and physical/chemical
 properties (Product Chemistry), and to characterize the product's acute
 toxicity (which is a function of its total composition).  The applicant for
 registration or reregistration of any product, whether it is a manufacturing-
 use or end-use product, and without regard to its intended use pattern, must
 submit or cite enough of this kind of data to allow EPA to evaluate the
 product.  For such purposes, "product specific" data on any product other than
 the applicant's is irrelevant,  unless the other product is closely similar in
 composition to the applicant's.  (Where it has been found practicable  to group
 similar  products for purposes of evaluating, with a single set of tests, all
 products in the group, the Standard so indicates.)  "Product specific" data on
 the efficacy of particular end-use products is also required where the exact
 formulation may affect efficacy and where failure of efficacy could cause
 public health problems.

 All other data needed to evaluate pesticide products concerns the properties or
 effects  of a particular ingredient of products (normally a pesticidally active
 ingredient,  but in some cases a pesticidally inactive, or "inert",
 ingredient).   Some data in this "generic" category are required to evaluate the
 properties and effects of all products containing that ingredient [e.g., the
 acute LD-50  of the active ingredient in its technical or purer grade; see
 proposed 40  CFR 163.81-1(a), 43 PR 37355].

 Other "generic" data are required to evaluate all products which both contain a
 particular ingredient and are intended for certain uses (see, e.g., proposed 40
 CFR 163.82-1,  43 FR 37363, which requires subchronic oral testing of the active
 ingredient with respect to certain use patterns only).  Where a particular data
 requirement  is use-pattern dependent, it will apply to each end-use product
 which is to  be labeled for that use pattern (except where such end-use product
 is  formulated  from a  registered manufacturing-use product permitting such
 formulations)  and  to  each manufacturing-use product with labeling that allows
 it  to be used  to make end-use products with that use pattern.  Thus, for
 example,  a subchronic oral dosing study is needed to evaluate the safety of any
manufacturing-use  product that  legally could be used to make an end-use, food-
 crop  pesticide.  But  if an end-use product's label specified it was for use
only  in ways that  involved no food/feed exposure and no repeated human
 exposure,  the  subchronic oral dosing study would not be required to evaluate
 the product's  safety;  and if a manufacturing-use product's label states that
 the product  is for  use only in  making end-use products not involving food/feed
 use or repeated human exposure,  that subchronic oral study would not be
 relevant  to  the evaluation of the manufacturing-use product either.

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If a registrant of a currently registered manufacturing-use or  end-use  product
wishes to avoid the costs of data compensation  [under FIFRA Section 3(c)(1) (D)]
or data generation [under Section 3(c)(2)(B)] for  "generic" data  that is
required only with respect to some use patterns, he may elect to  delete those
use patterns from his labeling at the time he reregisters  his  product.  An
applicant for registration of a new product under  this Standard may similarly
request approval for only certain use patterns.

E. Data Compensation Requirements under FIFRA 3(c)(1)(D)

Under FIFRA Section 3(c)(1)(D), an applicant for registration,  reregistration,
or amended registration must offer to pay compensation for certain existing
data the Agency has used in developing the Registration Standard.  The  data  for
which compensation must be offered is all data  which are described by all the
following criteria:

   1. The data were first submitted to EPA  (or  to  its predecessor
      agencies, USDA or FDA), on or after January  1, 1970;

   2. The data were submitted to EPA  (or USDA or FDA) by some other
      applicant or registant in support of an application for an
      experimental use permit, an amendment adding a new use to a
      registration, or for registration, or to  support or maintain in
      effect an existing registration;

   3. They are the kind of data which are relevant to the Agency's
      decision to register or reregister the applicant's product
      under the Registration Standard, taking into account the
      applicant's product's composition and intended use pattern(s);

   4. The Agency has found the data to be valid and usable in reaching
      regulatory conclusions; and

   5. They are not data for which the applicant has been exempted by
      FIFRA Section 3(c) (2) (D) from the duty to offer to pay
      compensation.  (This exemption  applies to the "generic" data
      concerning the safety of an active ingredient of the applicant's
      product, not to "product specific" data.  The exemption  is
      available only to applicants whose product is labeled  for end-
      uses for which the active ingredient  in question  is present in
      the applicant's product because of his use of another  registered
      product containing that active  ingredient which he purchases from
      another producer.)

An applicant for reregistration of an already registered product  under this
Standard, or for registration of a new product  under this Standard, accordingly
must determine which of the data used by EPA in developing  the  Standard must be
the subject of an offer to pay compensation, and must submit with his
application the appropriate statements evidencing  his compliance  with FIFRA
Section 3(c) (1) (D).

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 An applicant would never be required to offer to pay  for  "product specific"
 data submitted by another firm.  In many, if not in most  cases,  data  which is
 specific to another firm's product will not suffice to  allow EPA to evaluate
 the applicant's product, that is, will not be useful  to the  Agency in
 determining whether the applicant's product is registrable.   There may be
 cases, however, where because of close similarities between  the  composition of
 two or more products, another firm's data may suffice to  allow EPA to evaluate
 some or all of the "product specific" aspects of the  applicant's product.  In
 such a case, the applicant may choose to cite that data instead  of submitting
 data from tests on his own product, and if he chooses that option, he would
 have to comply with the offer-to-pay requirements of  Section 3(C)(1) (D)  for
 that data.

 Each applicant for registration or reregistration of  a  manufacturing-use
 product, and each applicant for registration or reregistration of an  end-use
 product, who is not exempted by FIFRA Section 3(c) (2) (D), must comply with the
 Section 3(c)(1)(D) requirements with respect to each  item of "generic" data
 that relates to his product's intended uses.

 A detailed description of the procedures an applicant must follow in  applying
 for reregistration (or new registration) under this Standard is  found in the
 Guidance Package for this Standard.

 F. Obtaining Data to Fill "Data Gaps"; FIFRA 3(c) (2) (B)

 Some of the kinds of data EPA needs for its evaluation  of the properties and
 effects of products to which this Standard applies have-never been submitted to
 the Agency (or, if submitted, have been found to have deficiencies rendering
 them inadequate for making reg 1strability decisions)  and  have not been located
 in the published literature search that EPA conducted as  part of preparing this
 Standard.   Such instances of missing but required data  are referred to in the
 Standard as "data gaps".

 FIFRA Section 3(c)(2)(B), added to FIFRA by the Congress  in  1978,  authorizes
 EPA to require registrants to whom a data requirement applies to generate (or
 otherwise  produce) data to fill such "gaps" and submit  those data to  EPA.  EPA
 must  allow a reasonably sufficient period for this to be accomplished.  If a
 registrant fails to take appropriate and timely steps to  fill the data gaps
 identified  by a section 3(c)(2)(B)  order, his product's registration  may be
 suspended  until the data is submitted.  A mechanism is  provided  whereby two or
 more  registrants  may agree to share in the costs of producing data for which
 they  are both  responsible.

 The Standard  lists,  in the third chapter, the "generic" data gaps  and notes the
 classes of  products to which these data gaps pertain.  The Standard also points
 out that to be  registrable under the Standard, a product must be supported by
certain required  "product specific" data.  In some cases, the Agency  may
possess sufficient "product specific" data on one currently  registered product,
but may lack such data on another.   Only those Standards which apply  to  a very
small number of currently registered products will attempt to state

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definitively the " product specific" data gaps on a "product by product"
basis.  (Although the Standard will in some cases note which data that EPA does
possess would suffice to satisfy certain "product specific" data requirements
for a category of products with closely similar composition characteristics.)

As part of the process of reregistering currently registered products, EPA will
issue Section 3(c)(2)(B) directives requiring the registrants to take
appropriate steps to fill all identified data gaps — whether the data in
question are "product specific" or "generic" — in accordance with  a schedule.

Persons who wish to obtain registrations for new products under this Standard
will be required to submit (or cite) sufficient "product specific"  data before
their applications are approved.  Upon registration, they will be required
under Section 3(c)(2)(B) to take appropriate steps to submit data needed  to
fill "generic" data gaps.  (We expect they will respond to this requirement  by
entering into cost-sharing agreements with other registrants who previously
have been told they must furnish the data.)  The Guidance Package for  this
Standard details the steps that must be taken by registrants to comply with
Section 3(c)(2)(B).

G. Amendments to the Standard

Applications for registration which propose uses or formulations  that  are not
presently covered by the Standard, or which present product compositions,
product chemistry data, hazard data, toxicity levels, or labeling  that do not
meet the requirements of the Standard, will automatically be considered by the
Agency to be requests for amendments to the Standard.   In  response  to  such
applications, the Agency may request additional data  to support  the proposed
amendment to the Standard, or may deny the application  for  registration  on the
grounds that the proposed product would cause unreasonable  adverse  effects to
the environment.  In the former case, when additional data  have  been
satisfactorily supplied, and providing that the data  do not indicate the
potential for unreasonable adverse effects, the Agency  will  then amend the
Standard to cover the new registration.

Each Registration Standard is based upon all data and  information available to
the Agency's reviewers on a particular date prior to  the publication date.
This "cut-off" date  is stated at the beginning of the second  chapter.   Any
subsequent data submissions and any approved amendments will be  incorporated
into the Registration Standard by means of addenda, which  are  available for
inspection at EPA in Washington, D.C., or copies of which may  be requested from
the Agency.  When all the present "data gaps" have been filled and the
submitted data have been reviewed, the Agency will revise  the  Registration
Standard.  Thereafter, when the Agency determines that  the internally
maintained addenda have significantly altered the conditions  for registration
under the Standard, the document will be updated  and  re-issued.

While the Registration Standard discusses only the uses and hazards of products
containing the designated active ingredient (s), the Agency is also concerned
with the potential hazards of some  inert ingredients  and  impurities.

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Independent of the development of any one Standard, the Agency has initiated
the evaluation of some inert pesticide ingredients.  Where the Agency has
identified inert ingredients of concern in a specific product to which the
Standard applies, these ingredients will be pointed out in the Guidance Package,
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                CHAPTER II: REGULATORY POSITION AND RATIONALE

  A. Introduction
  B. Description and Relationship of Chemicals
  C. Classification Under Minor Use
  D. Regulatory Position
  E. Regulatory Rationale
  F. Criteria for Registration Under the Standard
  G. Acceptable Ranges and Limits
  H. Required Labeling
  I. Tolerance Reassessment

A. Introduction

This chapter presents the Agency's regulatory position and rationale based  on
an evaluation of all registered products containing naphthaleneacetic acid,  its
sodium, potassium and ammonium salts, ester and acetamide as the sole active
ingredients. After briefly describing the naphthaleneacetic acid, the parent
chemical of this Standard, this chapter presents the regulatory position  and
rationale, and the criteria for registration of products containing this
chemical. These criteria include labeling considerations, and  tolerance
reassessment. A summary of data requirements is contained in chapter III. Data
supporting this regulatory position are discussed in each of the disciplinary
chapters, IV through VIII.

B. Description of Chemical

Naphthaleneacetic acid (NAA) , its sodium and potassium salts,  and its ester and
acetamide are plant growth regulators which are applied as either a ground  or
aerial foliar spray to apples, pears, olives, quinces, pineapples and
ornamentals .

The structural formula for these chemicals are:


                       0-H                               0~
                       I                                  I
                  H0-C-C=0                          H--C-CO
                   2 I                               2  I
               cAA
         Naphthaleneacetic Acid            Naphthaleneacetic Acid
                                                Sodium Salt

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                       0~  K                             0~
                        I                                  I
                   H0-C-C=0                          H0-C-00
                    2  |                               2  ,

               cAA                         
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The Agency estimates (Not derived from confidential business  information
estmiates) that the production of NAA and all its salts, ester and acetamide,
during the most recent five year period, is between 7,000 to  15,000 pounds of
active ingredient in all formulated products per year.
                                 11

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 Accordingly, these chemicals are determined to be minor use chemicals. As a
 result of this classification of minor use, many data requirements,
 particularly in the areas of Toxicology, Residue Chemistry, Environmental Fate
 and Ecological Effects have been waived in this Standard. Waiving these
 requirements is a result of a review of the use patterns and potential exposure
 to humans and wildlife, which indicates that exposure is likely to be minimal.

 D. Regulatory Position

 Based on a review of the available scientific data and other relevant
 information from the open literature and submitted by registrants as of June,
 1981 on naphthaleneacetic acid, its salts, ester and acetamide, the Agency has
 made the following determinations:

   1. Pesticide products containing these chemicals as active ingredients may
      be registered, subject to the terms and conditions specified in this
      Standard.

   2. None of the risk criteria found in section 162.11(a) of Title 40 U.S. Code
      of Federal Regulations has been met or exceeded for these active
      ingredients.

   3. No unreasonable adverse effects have been identified for these active
      ingredients when used in accordance with label directions and limitations.

   4. The registrant must develope or agree to develops additional data,
      specified in chapter III, to maintain the existing registrations or to
      permit new registrations.

   5. The existing tolerances cannot be reassessed at this time because of the
      limited residue data which are available. The limited residue data which
      are available indicate that the established tolerances will not be
      exceeded when use directions are followed.

 E.  Regulatory Rationale

 Product  chemistry,  ecological effects, toxicology, residue chemistry and
 environmental  fate  data available to support the registration of
 naphthaleneacetic  acid, its salts,  ester and acetamide are extremely limited.
 Therefore, no quantitative hazard or risk estimate could be performed for these
 chemicals.

 Exposure to  these chemicals may occur through consumption of residues retained
 in or on fruit and during  application and  mixing/loading of the formulated
product. Tolerances have been established  for NAA on apples, pears and quinces
at 1 ppm, on olives at  0.1 ppm and  on pineapples (as the sodium salt)  at 0.05
ppm. Tolerances have also  been established for the ethyl ester of NAA at 1 ppm
on apples and pears and 0.1 ppm on  olives.  Tolerance of  0.1 ppm have been
established for naphthaleneacetamide  on apples and pears.  These tolerances are
for NAA. Metabolites are not included in the  tolerance expression.
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Because residue data are insufficient to properly reassess these tolerances,
the Agency has decided to request residue data in plants. While existing
residue data are meager, the Agency currently has no reason to suspect that
exposure to NAA, via residues on food, will cause adverse effects. After
submission of the required residue data, the Agency will reexamine the current
tolerances.

Spray drift during application is not expected to be a major vehicle of
exposure because of the high use-dilutions of these chemicals. Mixing/loading
of these chemicals, because of contact with the concentrated product,  is
anticipated to result in significant respiratory, dermal and ocular exposure,
and is thus considered to be the major route of exposure. The use of goggles
and gloves will significantly reduce exposure during mixing/loading of end-use
products. Although dermal exposure to end-use products is high during
application to ornamentals, the methods of application and high use-dilutions
preclude significant exposure.

While data to assess the environmental fate of NAA do not meet the current
requirements, the Agency has determined that further environmental fate testing
is not required at this time because of the low application rates and  minor  use
status of these chemicals.

The Agency has not reviewed efficacy data for these chemicals because  their  use
does not affect the public health.

Although some data gaps remain, the Agency has concluded that  it should
continue the registration for this chemical for the following  reasons:

   1. No adverse effects data of regulatory concern have been  uncovered  in the
      review of the available studies which have been received, either in
      studies sufficient to meet data requirements or in those which fail to
      meet data requirements but which provide some basis  for a qualitative
      judgement.

   2. No significant effects have been reported  in the limited accident data
      for these chemicals.

   3. In accordance with FIFRA, the Agency's policy  is not to  routinely cancel
      the registration of products merely because we lack  data, or  to withhold
      registration merely for the lack of data.  (See sections  3(c)(2)(B)  and
      3(c)(7) of FIFRA.) Rather, publication of  the Standard provides a
      mechanism for identifying data needs, and  registration  under  the
      Standard allows for upgrading of labels during the period  in  which the
      required data are being generated. When these data are  received, they
      will be reviewed by the Agency. The Agency will then determine whether
      these data will affect the registration of this chemical.
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F. Criteria for Registration Under the Standard

To be subject to this Standard, products must meet  the  following  conditions:

  - contain naphthaleneacetic acid, its salts, ester  or acetamide;  as single
    active ingredients and

  - bear required labeling; and

  - conform to the acute toxicity limits, product composition  and use
    pattern requirements stated in section G below.

The applicant for registration or reregistration of products subject to this
Standard must comply with all terms and conditions  described in this Standard
including a commitment to fill data gaps on a time  schedule specified by the
Agency and, when applicable, offering to pay compensation  to the  extent
required by 3(c)(l)(D) of the Federal Insecticide,  Fungicide and  Rodenticide
Act (FIFRA), as amended, 7 U.S.C. 136(c) (1) (D). As  discussed in Chapter I,
applicants for registration under this Standard must  contact the  Registration
Division for specific instructions, including updated information contained in
the guidance package on data requirements and companies whose  data  must be
cited and to whom compensation must be offered.

G. Acceptable Ranges and Limits

  1.  Manufacturing-Use Products

     a. Product Composition Standard

     Currently the Agency has no information on acceptable ranges and limits
     for the product composition of manufacturing-use products containing these
     chemicals. To be covered under this Standard, manufacturing-use products
     containing these chemicals must propose acceptable  ranges and  limits for
     both active and inert ingredients.

    b.  Acute  Toxicity Limits

    The Agency will consider registration of manufacturing-use products
    containing  these chemicals regardless of the toxicity category,  provided
    that they bear  appropriate precautionary labeling.

    c.  Use  Patterns and  Application Methods

    To  be registered under  this standard, manufacturing-use products
    containing  these chemicals must be labeled to allow for formulation only
    into end-use products which are intended for use as:

        1) synthetic  plant growth  hormones for use on apples, pears, olives for
          thinning  of fruit,  and/or apples and pears to prevent  preharvest
          drop of fruit, and/or pinapples to induce flowering.
                                 14

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      2)  synthetic plant growth hormones for use on ornamentals to stimulate
         root growth, to delay leaf drop in holly, to prevent fruiting of
         ornamental olive, catalpa, elm, norsechestnut and maple trees, and
         to control resprouting from pruning wounds on certain deciduous
         trees.

2. End-Use Products

   a. Product Composition Standard

   Currently the Agency has no information on acceptable ranges and limits
   for the product composition of end-use products containing NAA, its salts
   ester  and acetamide. To be covered under this Standard, end-use
   products containing these chemicals must propose acceptable ranges and
   limits for both active and inert ingredients.

   b. Acute Toxicity Limits

   The Agency will consider registration of end-use products containing
   these  chemicals under a general-use classification, regardless of their
   toxicity category, provided that they bear appropriate precautionary
   labeling.
                               15

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      c.  Use Patterns and Application Methods

      Currently,  end-use products containing these chemicals are labeled as
      plant growth regulators for one or more of the uses specified in table 1.

                                  TABLE 1
               Use Sites for NAA,  its Salts, Ester and Acetamide
     PRODUCT TYPE

     DUSTS


     WETTABLE POWDERS
     EMULSIFIABLE
     CONCENTRATES

     SOLUBLE  CONCENTRATES/
     LIQUIDS
    SOLUBLE CONCENTRATES/
    SOLIDS

    CRYSTALLINE

    READY-TO-USE
    PRESSURIZED LIQUIDS
USE SITE

Apples
Ornamentals

Apples & Pears
Ornamentals

Apples & Pears
Apples

Pears

Olives
Ornamentals


Apples & Pears


Pineapples

Ornamentals
ACTIVE INGREDIENT

NAA only
Acetamide only

NAA, Sodium Salt, Acetamide
Sodium Salt only

NAA only
NAA, Ammonium & Potassium
Salts, Acetamide
NAA, Ammonium & Potassium
Salts
Potassium Salt only
NAA, Potassium & Ammonium
Salts

Sodium Salt only
Sodium Salt only

Ester only
Under this Standard, end-use products  containing  any of the chemicals covered
in this Standard may be  registered  for any of the sites listed in table 1,
provided that those uses are appropriately covered by existing tolerances.

H. Required Labeling

All manufacturing-use and end-use products' containing these chemicals must bear
labeling as specified in 40 CFR  162.10. The guidance package for this Standard
contains specific information  regarding label requirements. In addition, the
following specific labeling is required:
                                  16

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  1. Manufacturing-Use Products

  All manufacturing-use products containing these chemicals must bear one or
  both of the following statements on the label:

     "For reformulation into products intended as growth regulators of
     ornamentals."

     "For reformulation into products intended as growth regulators for
     apples, quinces, pears, pineapples, and/or olives."

  These statements are necessary to identify the applicability of  those  data
  requirements which are used to support manufacturing-use products intended
  for use on food crops.

  2. End-Use Products

  Except for those end-use products containing methanol or the ethyl  ester,
  there are no unique precautionary statements which must appear on labeling  of
  all presently registered end-use products containing the chemicals  covered  in
  this Standard.

     a. Methanol

  All end-use products containing methanol must bear the following statement:

                           "Contains methanol"

  All end-use products which contain greater than 4 percent methanol  must  bear
  the following additional statement:

                  "DANGER  (skull & crossbones) POISON"

          "Methanol may cause blindness. Harmfull or fatal  if
            swallowed. Vapor harmful. Avoid breathing spray,
                mist or vapors. Avoid contact with skin."

     b. Ethyl Ester

Based on data available on currently registered products,  soluble  concentrate
end-use products which presently contain 15.1% of the ethyl ester  of  NAA must
bear labeling indicating that the product  is corrosive to  the eyes and
irritating to the skin. Products presently containing this  chemical must bear
label recommendations for the use of goggles  (or face shield) and  gloves.

Refer to the guidance package for an updated list of all precautionary
statements which must appear on these types of products. The  Agency may, after
review of the data to be submitted under this Standard,  impose additional label
requirements.
                                  17

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I. Tolerance Reassessment

Tolerances have been established for NAA on apples,  pears,  and  quinces at 1
ppm; on olives at 0.1 ppm; and in or on pineapples at  0.05  ppm  (as the sodium
salt). Tolerances have also been established  for  the ethyl  ester  on apples and
pears at 1 ppm and on olives at 0.1 ppm. Tolerances  of 0.1  ppm  for the
acetamide of NAA have been established on apples  and pears. No  CODEX tolerances
exist for these chemicals.

Based on established tolerances, the theoretical  human exposure to residues is
calculated to be 0.0425 mg/dy/1.5 kg of diet. This figure is based on the
average adult eating patterns and on the assumption  that the foods contain
residues which meet the established tolerance levels.

Currently a tolerance of 1 ppm exists for NAA and its  ethyl ester. The limited
amount of residue data currently available in addition to theoretical estimates
of residues, indicate that residues of these chemicals are  significantly lower
than the present 1 ppm tolerance. The Agency has  determined that  some
additional residue data are required to confirm the  previously  measured
residues. If the requested data detect residues which  are higher  than those
previously measured additional toxicological data may  be required to support
the present uses. Any future requests for new uses and/or tolerances may result
in the requirement for additional toxicological,  environmental  fate, ecological
effects and residue chemistry data.
                                 18

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           CHAPTER III: SUf-MARY OF DATA REQUIREMENTS AND DATA GAPS

  A. Introduction
  B. Group A Charts: Generic Data Requirements
  C. Group B Charts: Manufacturing-Use Data Requirements
  D. Group C Charts: Ehd-Use Product-Specific Data Requirements

A. Introduction

Applicants for registration of end-use naphthaleneacetic acid, its  salts,  ester
and acetamide products must cite or submit the following information  on  the
physical/chemical properties, composition, fate, residue chemistry, ecological
effects and toxicity of the proposed product. Data in this Standard that
satisfy registration requirements may be cited,  if the applicant  establishes
that the proposed product is substantially similar to another product for  which
the Agency has received acceptable acute toxicity tests. Data may be  cited
provided compensation has been offered to the submitters of  these studies. The
Agency will consider both active and inert ingredients in the determination of
substantially similar products.  (See Chapter I for discussion of  substantially
similar products).

The section of the Proposed Guidelines which describes the type of  data  and
when it is required is listed before each requirement listed [43  FR,  29696 of
July 10, 1978; and 43 FR, 37336 of August 22, 1978]. Justification  for the
required tests is provided in the Guidelines. Areas where this Standard  differs
from the Guidelines are discussed in the Regulatory Rationale section of
Chapter II. Applicants for the reregistration of manufacturing-use  or end-use
products containing naphthaleneacetic acid, its  salts, ester and  acetamide must
submit all information identified as data gaps for manufacturing-use  and end-
use products, respectively (see charts). The footnotes to the charts  offer an
explanation of additional data requirements or data waivers  outside of the
guidelines. The data requirements specified are  the minimum  that  will be
required. Areas where additional data may be required as the result of tiered
testing are indicated.
                                  19

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                                                              DATA RTUHRfTIFWrS CHAW A
                                                    Hnplith.ilacctic Acid,  its s»Us, Ester i Anotnnldo
                                                    Oonnric mt» Rxjuircwnto: TOXIGOIflGY
Guiifr.'liiics
Citation
N.«TX- Oi Tt'St
Are mt.i Gompnaition
tequired?
Uccs Em have data
to pnrtially or
totally • tisfy
this roqutronmt?
Bibliographic"
Citation
Mint acllit i(*t if 
-------
                                                            DATA HFOuiwJiivrs CIIAJTT A
                                                  Nnphthalcntvicotlc Acid,  its Silts, Put or t
                                                   Generic Dttn R>qulrrmmts:RrSmir
"GulA'llnos NTC of Test
Citntion
Mctabdllan In riant*
Metabolism in Animals
Analytical ftthods
Residue Ditai
Crops- Apples
Are mta
Required?
yes
yes
yes
yos
Composition
Representative
formulations
Rnprntmntativc
Formulations
Rnpresontatlvo
Formulations
Rnpresentatlvr
Formulations
Docs nm have 
-------
                                                             DAW RRJUIREXmrS CHART B

                                                   NNphthalcncacotlc Acid, its Suits, Eater * Acctamidc

                                         Manufacturing-Use Data Requirements!  PRODUCT dimiSTRY
Guidel incs
Citation
163.61-3
163.61-4
163.61-5
163.61-6
163.61-7
163.64
Nome ol Test Arc Data
Required?
Product identity t yes
Disclosure of Ingredients
Description of yes
Hmuf.icturing Process
Discussion on Formulation yes
of Ui intentional Ingredients
Declaration t Certification yes
of mgrndicnts Limits
Product Analytical ftthods yes
t Data
Physical t Chemical yos
Properties
Composition
Bach Product
Bach Product
Ench Product
Rnch Product
Each Product
Itoch. Qrodo of A. I.
t F*ch Product
Docs BPA have data Bibliographic
to partially or Citation
totally sntlsfy
this requirement?
no
partial
no
no
no
no
Hist .-xWitlon.il d.11.1
be submitted ureVn.'
FIFRA 3(C)(2)(ni/
If so, clue wtun?
ycs/6 nmthfl i_ / £ /
yen/6 nantlis 2 / £ /
ycs/6 mnthn £ /
yos/6 «cntlis 2 / £ /
ycs/6 months
yes/ 4 /
those data requirements are current as of June.  1981.  Ifcfcr to tho guidance package fnc updated requirements.

 1.M1 impurities and Incrts in the technical  chemicals produced by or for Orocmeod Chemical  Gonpony,  Hillmnstcr Oiyx Cbrroratlon and Acrto
 Chemical Coupony must be identified.  Formulations of  all  other technical products and chemicals covered  by this Standard must be identified.

 Z.Oonplctc information must be supplied by each manufacturer  on the present manufacturing process,  including  data listed in 43 PR 29709.

 3.1he upper and low;r limits of each  active and inert Ingredient in manufacturing-use and cnd-uso products must be declared and cnrtifirxl. Ttv
 upper limit tor cnch impurity, reaction product nnd degradation product must be provided. If  not already cleared, all inert ingredients
 contained in products with food uses  must bo  cleared  by the registrant with the Food «nd nrug Administration.

 4.Those requirements must bo fulfilled by each  applicant. Data from other applicants may not  be cited. Therefore, even if tho roqulremmt hns
 been partially or completely fulfilled for some products, no  references are given. FScccpt for 163.61-7,  these requirements must be fulflllix!
 at the above dates.

                                                                                                                                        July. I"HI
                                                                     22

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                                                            DATA REcuiramn-s CHART c

                                                  Nnphthalenoacctic Acid.  its Salt*, Ester t Aorstomidc

                                            Bid-Use Product-Specific Data  Requirements: FRXUCT QIEMI.TmY
Guidelines
Citation
163.61-3

163.61-4

163.61-5

163.61-6

163.61-7

163.64

tl-no of TVst Arc Data Composition Docs EPA have data nihllogr.iphic Must txklitiai.il cMLj
Required? to partially or Citation be subroitt'-d urnV-r
totally satisfy FIFWV 3(c)(2)
-------
                                                           r«rm mcunvcms OIAW c

                                                 ttaffcthalcnoacatlc Arid, Its Salta,  Hrtcr ft Anotaakto

                                              BAd-Oas Raduct-flpnclflc tot* toqulroaentai
Guidelines Naao of lost Are Data
Citation ttoquirod?

SuUJHJK OONCEWIWITE8
A. Naphthalcneacctic Acid
GuafjuBition Dooa Cm navo data
to partially or
totally aatlafy
this raquiraaont?

Bibliographic taat •
Cltntion bo sub
PIPPA
If so,

rid it Una 1 Unta
•ittcrf under
3(c)(2)(n>?
duo «hm?

 163.81-4     Friaury eya Irritation      yas




 B. tetaaalua Salt of N«rfrth»JonoacoUc Acid

 163.81-4     Primary lyo Irritation      yea
C. Naphthalcncaoctic Acid. Ethyl Eater

 163.81-1    Acute Oral ttxiclty          yoa



 163.81-2    Acute Dcraal Ibxlclty        yoa



 163.81-3    Acute Inhalation Tt»icity    yea



 163.81-4    PrlHary Eye Irritation       yos
0. riaphthalcnoacctaaido
  163.81-4    rrlmnry FVD Irritation
                                                         •onulatlen
                                                    or flubatantially
                                                  Hteilar nraulatlona
                                                Containing >50% OUcetono
                                                    or ftitroloiai Oil
    Boob Mnulation
    or AlMtantially
  Similar nnulatlona
Ontalninq >25% laopropanol
                                                                           no
    Bieh Rjnnulation
    or flttetantially
  Siailar Itraulatlona

    Bach nraulation
    or eUatantlally
          •araulatlons
    Each Knulation
    or Sktatantially
  Stellar Ibnulatlona

    Bach Rrwlation
    or ftJMtantlally
  liaiilar tabulation*
                                                                           ye«
                                                                           yoa
                                                                           yoa
          Knwdation
    or adbatantlAlly
  SiaiUflr Rnulationa
ODntaining >50%  laooracanol
   and Mrtillacr
                                                                        yoa/t aantha
                                                                                                                        yoa/t aantha
                                                                                                    OB002303*
                                                                                                    OB0023032
                                                                                                    GB0023034
                                                                                                    (90023033
                                                                                                                        no
                                                                                                                        no
                                                                                                                         yoa/l aantha
These data requirements arc current an of Juno, IW1. Itofor to tho quidanao pocw»9O Cor updated roqulrcautta.
                                                                                                                                  July, mi

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                        CHAPTER IV: PRODUCT CHEMISTRY

  A. Product Chemistry - Manufacturing-Use Products
  B. Product Chemistry - End-Use Products
  C. Physical and Chemical Properties
  D. Analytical Methods
  E. Summary of Data Gaps

FIFRA 3(c)(2)(A) requires the Agency to establish guidelines for  registering
pesticides in the United States. We require registrants to provided
quantitative data on all added ingredients, active and inert, which are equal
to or greater than 0.1% of the product by weight.

To establish the composition of products proposed for registration, the Agency
requires data and information not only on the manufacturing and formulation
processes, but also a discussion on the formation of manufacturing  impurities
and other ingredients, intentional and unintentional, further, to assure  that
the composition of the product as marketed will not vary  from the composition
evaluated at the time of registration, applicants are required to submit  a
statement certifying upper and lower limits for the added ingredients, and
upper limits only for some unintentional ingredients. Subject D suggests
specific precision limits for ingredients based on the percentage of  ingredient
and the standard deviation of the analytical method.

In addition to the data on product composition, the Agency also requires  data
to establish the physical and chemical properties of both the pesticide active
ingredient and its formulations. For example, data are needed concerning  the
identity and physical states of the active ingredient  (e.g., melting  point  and
boiling point data, ambient vapor pressure and solubility). Data  are  also
required on the properties of the formulated product to establish labeling
cautions  (e.g., flammability, corrosivity or storage stability).  The  Agency
uses these data to characterize each pesticide and to determine its
environmental and health hazards.

A. Product Chemistry Manufacturing-Use Product

The technical grade of naphthaleneacetamide contains 98 percent of  the
acetamide.  The other technical grades contain 98 or 98.5 percent of
naphthaleneacetic acid or its sodium salt. The lack of identification of
impurities constitutes a data gap which must be filled. The physical/chemical
properties for technical 1-naphthaleneacetic acid and related chemicals covered
by this Standard are not available.  In addition, the  inert ingredients  in  many
manufacturing-use products containing these chemicals are not available and
constitute data gaps.

Several related chemicals (the ammonium, potassium and sodium salts,  the  ethyl
ester of naphthaleneacetic acid, and naphthaleneacetamide) are considered a
part of this Standard.  The lack of product chemistry data on these chemicals
constitutes a data gap. Product chemistry data must be submitted  on each
individual chemical.
                                  25

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 B. Product Chemistry - End-Use Products

 There is little product chemistry data on many end-use products. The lack of
 product chemistry data for these pesticides constitutes a data gap. Product
 chemistry data must be submitted for all end-use products containing these
 chemicals. The ingredients of all formulations must be identified to 0.1% in
 accordance with the proposed guidelines. Those ingredients which have been
 added intentionally to a food-use formulation must also be cleared under 40 CFR
 180.1001. The identification of the inert ingredients in many end-use products
 containing these chemicals are either not available or these ingredients have
 not been cleared as inert ingredients in pesticides used on food crops.
 Theseconstitute data gaps.

 C.  Physical and Chemical Properties

 The physical and chemical properties can serve as a means of chemical
 identification or as a confirmation of the reliability of other composition
 data. The physical and chemical properties of technical, manufacturing-use and
 formulated products containing NAA, its salts, ester and acetamide are
 generally not available and, hence, constitute data gaps.

 D.  Analytical Methods

 In  order to ensure that products contain only those percentages of active
 ingredients which they properly claim to contain, it is necessary to have
 analytical methods to determine the concentration of active ingredients in each
 formulation.  In addition, the commercial production of pesticide chemicals,
 reactions between pesticide ingredients, reactions with packaging materials, as
 well  as  degradation during the long period over which some products may be
 stored before use, can result in the formation of chemical impurities. Because
 of  the potential toxicity of these impurities, the Agency may require subroittal
 of  analytical methods for their assessment, both to improve the reliability of
 the Agency's hazard assessment and to ensure that marketed products conform to
 the standards of purity agreed upon by the pesticide producer.

 Analytical methods for the determination of the percentage of NAA in Fruit Fix
 Superconcentrate 800 (Crosby et al., MRID 000004943) and the percentage of the
 ethyl ester of NAA in Amchem Sprout Inhibitor A112 (Amchem Products, MRID GS-
 0023-012;  Amchem Products, MRID GS-0023-019; Amchem Products, MRID GS-0023-036)
 are inadequate by present scientific standards. The Agency has determined that
 validation data (e.g.  background values, recoveries, and method of sensitivity
 for the  active ingredient)  for all formulations and analytical methods for the
 identification and quantification of the active ingredients in all other
 formulations,  normally required for these chemicals, will not be required
because  of the minor use status of these chemicals.

E. Summary of Data Gaps

A summary of  the data  gaps for product chemistry are listed in Chapter III.
                                  26

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                         CHAPTER V: ENVIRONMENTAL FATE

  A. Use Profile
  B. Environmental Fate Profile - Manufacturing-Use Products
  C. Exposure Profile - End-Use Products
  D. Summary of EBta Gaps

A. Use Profile

Naphthaleneacetic acid, its salts, ester and acetamide are plant growth
regulators registered to (1) prevent pre-harvest drop of apples, pears,
quinces, olives, (2) thin fruit trees, (3) induce flower induction in
pineapples, (4) stimulate root growth on ornamentals and (5) delay leaf drop on
ornamentals. 7,000 to 15,000 pounds of active ingredient are produced per year
with the major portion used on apples and pears. Pineapples and ornamentals are
a small portion of the sites for these chemicals.

The abcission or lessening of uninjured fruits on a tree is a natural part of
the maturation process in fruit. The formation of a callous abcission
(separation) layer at the juncture of the fruit stem and the spur allows the
weakly held fruit to fall at maturity. NAA reduces release of fruit from trees
by slowing the formation of the abscission layer.

Because of its low solubility in water (0.042 g/100 ml), naphthaleneacetic acid
is usually sold in the form of a salt, ester or acetamide. Even though these
forms of NAA increase its solubility, NAA is reformulated with various inert
ingredients (alcohol, acetone, etc.) to promote even dispersal in use dilutions
of end-use products. These chemicals are formulated into dusts, wettable
powders, emulsifiable concentrates, soluble concentrates/liquid, soluble
concentrates/solids, granulars, pressurized liquids and ready-to-use liquids.
The percentage of active ingredient in these formulations varies from 0.002 to
98 percent.

These chemicals may be applied by either ground and aerial application
equipment at a concentration of approximately 10 ppn, but it may be necessary
to vary the concentration of these chemicals to obtain good drop control of
certain varieties of fruit. Application is made approximately 10 days prior to
picking or at the first sign of sound, fully seeded fruit dropping from the
tree. longer periods of drop control may be obtained in apples by spraying 14
to 20 days prior to picking with a repeat application seven to ten days later.

Coverage of the entire fruit tree is essential because these chemicals are
absorbed through the foliage and translocated only short distances within the
tree stems to the fruit. Application of these chemicals is usually accomplished
by ground rigs from air-blast sprayers. Approximately 1500 to 2000 gallons of
spray solution is applied on three to five acres per hour. A maximum of 70
acres of mature fruit bearing trees can be covered in a ten hour day. The
timing and rate of application is very critical because these chemicals require
approximately three days to become effective and their effectiveness lasts from
                                 27

-------
 10 to 14 days.  Premature  application will not prevent pre-harvest drop because
 their effect  will  dissipate  before drop begins. Late application will deliver
 little or no  benefit.  To  maintain effectiveness, application should be made at
 temperatures  above 70  F and  when rain will not occur for several hours.

 The thinning  of fruit  from trees is an essential part of orchard management.
 The process of  thinning involves the removal, by hand or chemical spraying, of
 immature fruit.  Thinning  results in larger, more uniform fruit size, reduces
 the size of fluctuations  between harvests for biennial bearing trees, reduces
 limb breakage,  and maintains tree vigor.

 Historically, thinning was performed by removing, by hand, of immature fruit
 after a natural drop.  Depending  on the anticipated crop size, growers attempted
 to strive for a uniform number of fruit per tree, or per cluster on a tree.

 Thinning with chemical agents, such as NAA, provides a means to partially or
 completely overcome the necessity of hand thinning and has now become a
 standard commercial practice in  many fruit growing areas of the U.S.

 Chemical thinning  agents  are usually sprayed while the fruit is still
 susceptable to  the thinning  effect, but after the leaves have reached a point
 in their development where injury to foliage would be least likely to occur.
 Because the results of thinning  are highly variable, directions for use will
 depend on the weather, amount of bloom, pollination, rate of application, time
 of application  and region of the country where the chemical is used.

 The effectiveness  of chemical thinning also depends upon the rate of absorption
 by the leaves of the fruit tree. Absorption of thinning agents will vary
 between fruits  and between varieties of a fruit as well as whether a wetting
 agent is used during application. Concentrations of these chemicals may be
 lowered by 50%  the recommended rates when a wetting agent is used in the spray
 solutions. Finally,  insufficient thinning one year does not necessarily warrent
 an increase in  the concentration the next year.

 Thinning may be  performed with either high or low volume equipment. The main
 objective  of  effective spraying  is calculation of proper dosage rates and
 application of  these chemicals to each tree so that each tree is thouroughly
 covered.  Failure to perform  proper dosing and uniform coverage may result in
 low thinning activity.  Spray concentrations of up to 20 ppm are used for
 thinning  apples, up to 50 ppm for pears, and up to 150 ppm for thinning olives.

 NAA is  also used to  induce flowering of pineapples at a rate of 25 grams A.I.
 per  acre applied as  a  spray  solution. It is applied when most of the crop is
 still  in the vegetative stage. No more than two applications are made to the
 crop.

 B.  Environmental Fate  Profile

 Limited  data are available to assess the environmental fate of
naphthaleneacetic  acid  (NAA).
                                  28

-------
Asperqillus niger appears to metabolize NAA to 5-hydroxy NAA.  Other  possible
products are the 4- and 6-hydroxy NAA isomers, as well as other phenolic or
naphtholic compounds (Clifford & Woodcock, MRID 005011538). Because the
metabolites identified in these studies are structurally related/ hydroxylation
thus appears to be a major route of degradation of NAA.

A coliform bacterium and two animal pathogens  (Staphylococcus aureus  and
human type tubercle bacilli) are inhibited by NAA at 50 to 300 ppm, where  as
avian type tubercle bacilli are not inhibited by NAA at 50 ppm  (Loveless et
al., MRID 005005277) (Ukita et al., MRID 005010797). A yeast, Saccharomyces
cerevisiae, is inhibited 13 to 58 percent by NAA at 100 to 500 ppm.   In
addition, large-cell mutants result from the induction of polyploidy  (Doi  et
al., MRID 005008826). Two mutants of Neurospora crassa are inhibited  12 to 68
percent by NAA at 10 to 50 ppm, but a wild type control strain was not studied
(Klein, MRID 005010966). An algae, Chlorella pyrenoidosa, is  inhibited 40  to
80 percent by NAA at 46 to 372 ppm (Grandich & Frans, MRID 005009027).

Thus, a wide range of microorganisms are inhibited by NAA at  10 to 500 ppm
which are concentrations far in excess of the highest expected level  in the
soil (0.08 ppm). Most studies dealt with mutants, animal pathogens, or soil
microorganisms and therefore are of questionable importance. While none of the
studies reported the source and purity of the NAA used, these studies are
sufficient to provide an indication that high concentrations of NAA will
inhibit microorganisms.

In summary, although very little data are available to form a profile of  the
environmental fate of NAA, 5-hydroxy NAA is a suspected fungal metabolite  of
NAA.  At very high concentrations, NAA is capable of inhibiting bacteria,
fungi, and algae and also of inducing polyploid mutants.  The available data
suggest that currently approved NAA application rates will not have an effect
on soil microorganisms.'

Based on the minor use status of these chemicals and because  the  Agency  would
not normally require the above data on microorganisms, the above  studies  are
for information only and need not be cited or  submitted by applicants.

No other data exist on the environmental fate of these chemicals. Because these
minor use chemicals, are applied at very low application  rates  (25 to 35 grams
per acre), and are applied only once or twice  per year, the Agency has
determined that exposure to humans and wildlife is  likely to  be minimal.
Accordingly, further environmental fate data requirements have been waived.

C. Exposure Profile

 1. Introduction

1-Naphthaleneacetic acid  (NAA) is registered for use on orchard  crops and
ornamentals as a growth regulator.  Its total  annual use  is between 7,000 to
15,000 pounds. Thirty to fifty percent of this production is  used primarily on
apples and 20 to 30 percent of this production is used on pears.  Ten  to
thirtypercent of the yearly production account for  ornamental uses, with use on
olive and pineapple crops each accounting for  less  than 10 percent of the


                                 29

-------
pounds of  this  production.   On apples and pears, NAA is applied to chemically
thin the fruit  or  to  prevent preharvest drop.  Ornamental uses are mainly for
root growth  stimulation and for delaying leaf drop. The present formulations of
these chemicals are listed  in table 2.

                                   TABLE 2

        REGISTERED FORMULATIONS OF NAA, ITS SALTS,  ESTER & ACETAMIDE

   CHEMICAL                            FORMULATION  TOPE
                   D        EC         SC/L      SC/S   WP   RTU   CR    PrL
                  (%ai)  (Ib/gal ai)  (Ib/gal ai)  (%ai)  (%ai) (%ai)  (%ai)  (%ai)

  NAA              072   oTol6       0.04-0.28          375

  Sodium Salt                                 3.5-98  2.0 &        98
                                                       7.11
  Potassium  Salt

  Ammonium Salt                     0.44 &
                                     1.76
  Ethyl Ester                                               1.0         1.0

  Acetamide        0.4               0.176              8.4

D=dust; EC=  emulsifiable concentrate; SC/L= soluble concentrate/liquid; SC/S=
soluble concentrate/solid;  WP= wettable powder; RTU= ready-to-use; CR=
crystalline;  PrL=  pressurized liquid; IM= impregnated material
NAA formulations (D,  WP, EC, SC/L, SC/S, and Cr)  are applied as foliar sprays
to fruit crops, either  aerially or using ground equipment that directs the
spray up into the  trees. Applications for ornamental uses include soil drench
(SC/L), manual  spraying (WP and SC/L), and dipping  plant cuttings  (D and SC/L).

 2. Foliar Applications

No data are  available to assess the environmental fate of NAA and subsequent
potential  for exposure  to humans and wildlife. Similarly, no data are available
to quantify  the exposure that applicators and their helpers may experience in
the handling  and application of NAA.

All formulations,  except the RTU and PrL formulations, are registered for use
as foliar  sprays.  While spray drift from high-pressure spray equipment and
aerial applications may expose humans, livestock, and wildlife outside of the
application site,  the major exposure will be to workers mixing, loading, and
applying NAA. Respiratory and dermal exposure may result from "puff back" of
the dry formulations  during mixing; ocular exposure could occur during mixing
of the liquid formulation.  The use of protective clothing should eliminate any
hazard associated  with  this route of exposure.
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These chemicals are usually applied by a  single  person  using  air  blast
equipmentcontrolled from a tractor for 8  to 10 hours per day. Mixing/loading  of
the chemicals and refueling of the tractor occurs approximately 12  to  15 times
per day, averaging 10 minutes per stop. A second individual,  driving a supply
truck, may be involved in application. This second  individual may be exposed  to
the spray drift or solution for not more  than 5  to  10 minutes for each hour of
spraying.

The air blast equipment used to with these chemicals will  apply 200 to 300
gallons per acre, with spray particles reaching a height of up  to 25 feet,
depending on the orientation of the upper bank of nozzles, air  direction vanes
on the top of the sprayer, wind conditions, etc.

Regular work clothes are usually worn by  the tractor driver and the refilling
helper. Coveralls may be worn by some workers and rubber gloves may be worn by
individuals handling the concentrated product. Approximately  25%  of the tractor
operators work in enclosed cabs.

 3. Soil Drench and Dip Applications

Ornamental uses of NAA include a soil drench to  stimulate  rooting and  a dip for
plant cuttings.  Dermal exposure would result to workers during these
procedures.  However, such exposure will  be extremely small considering the
small amount of active ingredient found in use dilutions of these chemicals.
The half-life of NAA in potting soil and  on cut  plant surfaces  is not  known.

D. Summary of Data Gaps

Because of the low application rate and the minor  use status  of these  chemicals
the Agency has determined that further environmental  fate  testing is not
required.
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                             CHAPTER VI: TOXICOLOGY

   A. Toxicology Profile
   B. Human and Domestic Animal Hazard Assessment
   C. Summary of Data Gaps

 A. Toxicology Profile

   1. TECHNICAL CHEMICALS

      a. Napthaleneacetic Acid  (NAA)

         1) Acute Effects

 No data were available to assess the acute oral, acute dermal and acute
 inhalation toxicities of technical NAAf nor are data available to assess the
 primary eye irritation, primary dermal irritation, and dermal sensitization
 potential of technical NAA. The above acute toxicity tests must be performed on
 technical NAA and are so identified in Chapter III of this Standard.

         2) Subchronic Effects

 In a range-finding study (Huntingdon Research Center, MRID GS-0023-001) male
 and female Sprague-Dawley rats were given 250, 1000 or 4000 mg/kg/day technical
 NAA by gavage for 10 consecutive days. All the animals at the highest dose and
 one female in the 1000 mg/kg/day dose group died after two to three days.
 Clinical signs of toxicity which preceded death, included dyspnea, ataxia,
 lethargy and prostration. Dose related depressions in body weight gain and food
 consumption were observed.  The incidence of gross pathological alterations,
 which included discolored lungs, liver or kidneys, distended bladder and G.I.
 tract,  was greater in the treated groups. From the doses tested, 250 mg/kg/day
 appeared to be the maximum tolerated dose.

 In another range finding study (Verschuuren et al., MRID 005008106) male and
 female  Wistar rats were fed diets containing 0, 50, 250, 1,250 or 7,500 ppm
 technical 1-NAA for two weeks. Evidence of toxicity included liver glycogen
 depletion at the two highest doses, accompanied by a slight increase in
 relative liver weight at 7500 ppm and a nonsignificant increase in serum SGPT
 activity at this dose.  In addition, an increase in the relative kidney weight
 at 1250 and 7500 ppm was significant in males. Histological examination
 demonstrated some changes in the tubular epithelial cells of the kidneys in
 rats  at the 2 highest doses. These changes in the renal tubules were indicative
 of proteinosis which is caused by the leakage of proteins from the glomeruli
 and subsequent resorption in the proximal tubes.

 Verschuuren et al.  (MRID 005008106)  also investigated the effect of feeding
 technical  1-NAA (% A.I.  unspecified)  in the diet at 0,  200, 1000 or 5000 ppm to
male and  female  rats  for 90  days.  This study provided supplemental information
 about the  subchronic  toxicity of 1-NAA in rats. A reduction in growth and food
consumption was  observed  in  males  at the  highest dose and was accompanied by an
 increase  in the  relative  liver weight  and an induction of liver microsomal
enzymes  in  these same animals.  These results demonstrated a dose related


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reduction in liver glycogen depletion  (which was more severe  in  females)  and  a
centrolobular decrease in liver glucose-6-phosphatase at all  levels. Because  of
these findings, a NOEL could not be determined from this study.  The authors
indicated that the renal tubular changes observed in the two  week range  finding
study were not observed at 200, 1000 or 5000 ppm in the 90 day study.

The subchronic oral toxicity of technical NAA in rats could be adequately
assessed in a 90-day feeding study (CDC RESEARCH, MRID GS-0023-002) in which
male and female Sprague-Dawley rats were given 0, 50, 150 or  500 mg/kg/day of
technical NAA in the diet. In this study, body weight gain depression
(approximately 12 to 13%) was observed in males and females at 500 mg/kg/day.
In addition, a significant increase in both relative and absolute liver  weight
was observed in females at the highest dose. The data indicated  a NOEL of 150
mgAg/day. The authors reported that there was no evidence microscopically of
hepatic centrolobular glycogen depletion as reported previously  (Veschuuren et
al., MRID 005008106). This study fulfills the subchronic oral toxicity data
requirement in rats.

In a six-month subchronic oral toxicity study (Elars Bioresearch Laboratories,
MRID GS-0023-031) technical NAA (in gelatin capsules) was given  to male  and
female beagle dogs at doses of 0, 50,  150 and 300 mg/kg/day.  Glycogen
depletion and degenerative changes in  the liver were observed at 150 and 300
mg/kg/day.  Also, two of eight dogs at 50 mg/kg/day showed slight evidence of
pericholangitis that was similar in nature to lesions in the  livers of dogs
given 150 and 300 mg/kg/day.  This study indicates that the dog  is more
sensitive to the toxic effects of NAA  than the rat. A NOEL of 50 mg/kg/day  is
established from this study based on the following considerations:

          - pericholangitis at the lowest dose level was slight  and  reflects  a
           very low to no effect on liver function,

          - the lesion is reversible and reflects a slight inflamation process
           resticted to the portal areas of the liver

          - while dose related, the lesion is considered to be mild.

No further subchronic oral testing in  the dog is required.

An adequate study was available to assess the teratogenic potential  of
technical NAA in rats.   (Huntington Research Center, MRID GS-0023-044).   In
this study technical NAA at concentrations of 0, 10, 50 or 250 mgAg  (in
supension with 0.05% sodium carboxymethylcellulose) was given (by gavage) to
pregnant CD rats on gestation days 6 through 15.  No teratological  effects  were
observed.  A decrease in maternal body weight was observed at 250 mg/kg,
however this was not accompanied by fetotoxicity.  The data  indicated  that  the
NOEL for teratology for NAA technical  in rats is greater than 250 mg/kg.  The
NOEL for maternal toxicity was 50 mgAg.  This study fulfills the  teratology
data requirement for NAA in one mammalian species. Testing  in a second species
is required.
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         3)  Chronic Effects

 In a 19 month oncogenicity study (Bionetics Research Labs, MRID 005010016) NAA,
 at 100 mg/kg by a single subcutaneous injection (in DMSO) or at 215 mg/kg by
 oral intubation (in 0.5% gelatin)  for 21 days and in the diet at 517 ppm for
 the remainder of 18 months, was evaluated for carcinogenicity in two hybrid
 strains of mice, B6C3F1 and B6AKF1.  No significant difference was observed
 between the tumor incidence of mice treated with NAA and mice in the negative
 control groups.  However, the number of animals tested in each group was too
 small to adequately evaluate the carcinogenic potential of NAA in mice.  In
 addition, the sensitivity of this assay is diminished since, one of the
 chemicals used as a positive control, isosafrole, gave equivocal evidence of
 oncogenicity. While this study does not meet the Agency's current requirements
 for oncogenicity testing, it does provide an initial screen of the oncogenic
 potential of NAA. Since positive results were not demonstrated and since these
 chemicals are minor use chemicals, the Agency has determined that further
 oncogenicity testing will not be required at this time.

         4)  Mutagenic Effects

 Adequate data were available to assess the mutagenic potential of technical 1-
 NAA in bacterial assays.  In a study, (Pharmakon Laboratories, MRID GS-0023-
 041)  1-NAA at concentrations of 0.5, 2, 8, 40, 200, 1000 or 5000 ug/plate was
 non-mutagenic in Salmonella typhimurium  strains TA 1535, TA 1537, TA 1538, TA
 98 and TA 100 when tested with and without metabolic activation.  This assay
 detects reverse mutations resulting from base pair substitution or frameshift
 events.  The data can be considered sufficient to fulfill the mutagenicity data
 requirement for microbial point mutation testing.

 In another  study, (Pharmakon Labortories, MRID GS-0023-040)  technical 1-NAA (in
 water or 10% DMSO)  at concentrations of 10, 20 and 40 ug/plate did not produce
 a  zone of inhibition when added to cultures of Escherichia coli DMA repair
 deficient strain P 3478 or DMA repair competent strain W3110, with and without
 metabolic activation.  Positive controls, ethylmethanesulfonate and
 diethylnitrosamine did cause zones of inhibition under similar testing
 conditions.   The lack of a zone of inhibition with 1-NAA in this assay should
 be interpreted as demonstrating that NAA does not interact with DMA in
 Escherichia  coli.

 Studies were available to assess the effects of technical 1-NAA in yeast.
 (Pharmacon  Laboratories, MRID GS-0023-037; Pharmacon Laboratories, MRID GS-0023-
 038;  Pharmacon Laboratories, MRID GS-0023-039).  In these assays, technical 1-
 NAA was evaluated  for its effects  on mitotic crossing over,  reverse mutation
 and gene conversion in Saccharomyces cerevisiae strain D-7.
                                                              —6     —2
 In the  mitotic crossover assay, 1-NAA at concentrations of 10  to 10   M
did not  have mutagenic activity in heteroallelic ade 2-40/ade 2-119 diploid
strain D-7 Saccharomyces cervisiae,  when tested without metabolic activation.

In a  reverse mutation assay,  1-NAA at concentrations of 10  to 10  n did
not have mutagenic  activity in homoallelic ilv - I - 92/ilv - I- 92 diploid
strain D-7 Saccharomyces cervisiae,  when tested without metabolic activation.


                                 34

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In the mitotic gene conversion assay 1-NAA at concentrations of 10  to 2.1
xlO  M did not have mutagenic activity in heterallelic diploid trp 5-12/trp 5-
27 strain D-7 Saccharomyces cervisiae, when tested without metabolic
activation.

The above assays indicated that 1-NAA was nontoxic to the yeast at
concentrations as high as 10  M. This finding and the lack of genetic
activity in all three assays indicate that 1-NAA is inactive in Saccharomyces
cervisiae strain D-7 yeast.

In another study (Doi et al., MRID 005008826), NAA was evaluated for its
ability to induce large cell mutants in Saccharomyces cerevisiae haploid strain
H 40. This study suggests that NAA, at 500 ug, induced changes in the ploidy of
these cells. These data cannot be interpreted as indicating that NAA has
genetic activity in this strain of yeast because a change in ploidy does not
necessarily reflect an effect on DNA.

Supplementary data were available to evaluate the ^n vivo cytogenetic effects
of technical 1-NAA  (Pharmakon Laboratories, MRID GS-0023-042). In a study, male
and female mice were pretreated for two days with 60 or 125 mg/kg of NAA  (in
water) by intraperitoneal injection. Six hours after the second dose the
animals were killed, their femurs were removed and the bone marrow aspirated.
Polychromatic erythrocytes were counted for the presence of micronuclei  (an
indication of chromosomal breaking effects of the chemical).  The results  for
NAA were negative while TEN  (triethylenemelamine), the positive control, gave  a
statistically significant increase (over vehicle control) in the number of
micronuclei per 1000 polychromatic erythrocytes. Since no cell counts were
made, the sensitivity of this assay is diminished. It is possible that NAA was
toxic to the cells; in this event, an increased number of micronuclei may not
have been, detected.

A dominant lethal study with technical 1-NAA  is available  (Pharmakon
Laboratories, MRID GS-0023-043). In this study male  rats were given 1-NAA in
0.25% methylcellulose by oral intubation at concentrations  of  125,  250  or 500
mgAg for fiye consecutive days. Subsequently the males were mated with virgin
females. Ma tings were repeated  for a  total of eight  weeks.  Vehicle  and
positive control (triethylenemelamine  (TEN) groups were run under similar
testing conditions. The results indicated that NAA did not  produce  dominant
lethal effects in male rats as measured by preimplantation  and post-
implantation losses. Post-implantation fetal  deaths  were significantly
increased after the first four matings in dams mated to males given TEN,  the
positive control. This study is adequate to assess  the dominant  lethal effect
of NAA. It can be concluded that NAA, at concentrations as  high  as  500 mg/kg,
does not produce dominant lethal effects in rats.

The above identified studies are adequate to  evaluate NAA  for  its mutagenic
potential. Although some of the studies, by themselves, were insufficient to
assess the mutagenic potential of NAA, as a whole the data indicate that NAA
has a low potential for inducing genetic effects. Further  mutagencity testing
with NAA, its salts, ester and  acetamide  is not required.
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         5) Metabolic Effects

An adequate  metabolism study is available with carboxyl-labeled
naphthaleneacetic  acid Ci4(l-NAA-C14)  in rats. (Letcho and Brouwer, MRID
000004942).  In  this  study,  male rats were given single oral doses of 0.1, 1.0,
100 or 250 mg of 1-NAA-C   (in sodium hydroxide).  Radioactivity in the urine
and feces was monitored daily for three days. The  results indicated that 71 to
90% of the radiolabel was excreted in the urine and 3 to 21% was excreted in
the feces within three days. The two major urinary metabolites (70 to 93%)  were
naphthaceturic  and naphthacetyglucosiduronic acids. It was observed that at the
lower doses, approximately 90% of the radioactivity was excreted in the urine
in the first 24 hours.

In this  study,  rats  with cannulated bile ducts were given single oral doses of
0.1 or 100 mg of 1-NAA-C  . The radioactivity of the urine and bile was
monitored every two  hours (up to six hours). The results showed that 4 to 21%
of the radiolabel  collected during this time was biliary, while 1 to 53% of it
was urinary. At the  high dose, the major metabolite was the glucuronic acid
conjugate  (i.e. naphthylglucosiduronic acid) while at the low dose the major
metabolite was  the glycine conjugate (i.e. napthylaceturic acid). In summary,
this study indicated that 1-NAA and its metabolites are rapidly excreted in the
urine and feces of rats* In addition, it was shown that rats have a greater
capacity for glucuronic acid conjugation.

Additional information concerning the metabolism of 1 NAA-C   in man,
primates, rodents, cat and bat was available  (Dixon et al., MRID 005008052). In
this study 100  mg/kg of  1-NAA-C   (in sodium hydroxide) was given
intramuscularly to 6 primate species, intraperitoneally to the cat, rat and
bat, and orally to the rabbit. In addition,  5 mg of 1-NAA-C   (orally) was
given to a man.  In most species tested 60 to 100%  of the radioactivity was
excreted in  the urine by 48 hours. The glucuronic  acid conjugate was the major
urinary  metabolite in man,  rhesus monkey, marmoset, rabbit, rat and fruit bat.
In the cat,  no  glucuronic acid conjugate was excreted; taurine and glycine
conjugates were the  major excretion products.

Also, in this.study  female rats with cannulated bile ducts were given 5 to 500
mg/kg 1-NAA-C    intraperitoneally. After three hours, 10 to 44% of the
radioactivity was  present in the bile and 0.6 to 32% was present in the urine.
At the highest  doses, glucuronic acid conjugation  predominated whereas at the
lower doses  there  appeared to be a similar amount  of glycine and glucuronic
acid conjugation.

The above data  are adequate to characterize the metabolism of 1-NAA. It is
expected that the  ethylester of 1-NAA and naphthaleneacetamide will be
metabolized  similarly once  they are degraded to their respective alcohol and
salt, and NAA by gastro-intestinal esterases and amidases. No additional
metabolism studies are required with these chemicals.
                                  36

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     b. Salts

        1) Acute Effects

No data were available to assess the acute oral, acute dermal and acute
inhalation toxicities of the technical sodium, potassium or ammonium  salts of
NAA.  Since it is expected that the acute toxicities of the sodium and
potassium salts of NM will be comparable to those of the acid, testing with
these chemicals is not required. The acute toxicity of the ammonium salt will
be assessed once the data are available for the acid.

No data were available to assess the primary eye irritation, primary  dermal
irritation and dermal sensitization potentials of the technical sodium,
potassium or ammonium salts.  It is expected that sodium and potassium salts of
NAA will have similar eye and dermal irritation potentials and dermal
sensitization potentials as the acid; therefore, testing of these chemicals  is
not required.

The ammonium salt is not expected to cause dermal irritation or dermal
sensitization greater than that resulting from exposure to the acid;  therefore,
these tests are not required.  However, the ammonium salt may have eye  irritant
properties that are greater than those caused by exposure to the acid;
therefore, an eye irritation study with this salt is required.

        2) Subchronic/Chronic/Mutgenicity Effects

Subchronic, chronic and mutagenicity toxicity testing with the technical
sodium, potassium or ammonium salts is not required, since the subchronic,
chronic and mutagenic toxicity of these chemicals is expected to result  from
exposure to the acid.

     c. Ester

        1) Acute Effects

An adequate acute oral toxicity study in rats  (Affiliated Medical Research,
MRID GS-0023-048) was available with technical NAA- ethyl ester.   In  this
study, NAA-ethyl ester in water, was given to three groups of male  rats by oral
intubation.  The LD5Q was determined to be 3580 + 333.3 mg/kg.  Gross autopsy
findings from the animals that died during the study  included hemorrhagic
stomach, intestines and lungs, fluid and gas filled intestines and  stomach,
discolored spleen and kidneys, mottled liver, dialated mesenteric  blood
vessels, wet genital area and bloody nose.  These data are sufficient to
fulfill the acute oral toxicity data requirement for  the  technical  ethyl ester
of NAA and place this product in Toxicity Category III.

An adequate acute dermal toxicity study in rabbits with  technical NAA ethyl
ester was available.  (Affiliated Medical Research, MRID  GS-0023-047).  In this
study the acute dermal LD5Q in male albino rabbits was determined  to be
greater than 5 g/kg.  No mortality was observed when  four male  albino rabbits
were given 24 hour dermal exposure to 5 gAg of technical NAA-ethyl ester.  The
                                  37

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 test material was applied to the shaved, unabraded skin under an occlusive
 rubber wrap.  No signs of toxicity or gross pathological alterations were
 observed.  These data are sufficient to fulfill the acute dermal toxicity data
 requirement for the technical ethyl ester of NAA and place this product  in
 Toxicity Category III.

 Sufficient data were available to assess the acute inhalation toxicity of
 technical NAA ethyl ester in rats.  (Affiliated Medical Research, MRID GS-0023-
 046).  In this test, the LC5Q was determined to be greater than 206.5 mg/L.
 No mortalities were observed when six male albino rats were given one hour
 inhalation exposure to a nominal concentration of 206.5 mg/L of aerosol
 technical ethyl ester of NAA in a 50% suspension with water.  Following
 exposure, signs of toxicity were irritation and lethargy.  The animals appeared
 normal by the second day.  No gross pathological alterations were observed.
 These data are adequate to fulfill the acute inhalation toxicity data
 requirement and place technical ethyl ester of NAA in toxicity category  IV.

 The above data indicate a low acute toxicity potential for the ethyl ester in
 humans.

 Adequate data were available to evaluate the primary eye irritation potential
 of technical NAA-ethyl ester.  (Affiliated Medical Research, MRID GS-0023-
 045). In this study, 0.1 ml of the test chemical was instilled into the  left
 conjunctival sac of the eyes of nine male albino rabbits. Six eyes were  washed
 two or four seconds after instillation.  No irritation was observed to the
 cornea, iris or conjunctiva in washed and unwashed eyes.  The primary eye
 irritation score was 0 (out of a possible 110).  These data are sufficient to
 fulfill the primary eye irritation data requirement for the technical ethyl
 ester of NAA.   The chemical should not be considered a primary eye irritant and
 it should be placed in Toxicity Category IV.

 Since the ethyl ester did not produce eye irritation in rabbits and since no
 gross pathological skin alterations were observed in rabbits in an acute dermal
 toxicity study with this chemical, it is expected that the ethyl ester will
 have  a low potential for dermal irritation in humans. No further dermal
 irritation testing is required.

 No data were available to assess the dermal sensitization potential of the
 ester.   It is  expected that the dermal sensitization potential of this ester
 will  be similar to that of the acid; therefore, additional testing is not
 required.

         2)  Subchronic/Chronic/Mutagenic Effects

 In an inadequate  study by- Finch and Hartzell (MRID 005022160), female mice were
 fed diets  containing 0,  0.95,  9.5,  29 or 86 ppm of alpha naphthalene acetic
 acid  methyl ester  for 1-1/2 to 2 years. No significant differences in body
 weight  gain or survival  and no pathological alterations attributed to the test
material were  reported.   The study was insufficient to assess the chronic
                                  38

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toxicity of NAA; the number of animals treated at each dose level was low (20
mice/dose) and only females were tested.  Animals were housed in group cages
and weighed by group; individual body weights were not determined. There  was no
food consumption data, clinical testing was not performed, and
histopathological examinations were not made.

Finch and Hartzell (MRID 005022160) also investigated the reproductive effects
of alpha naphthalene acetic acid methyl ester in the diet at concentrations of
0, 0.95, 9.5 29 or 86 ppm in mice. No reproductive toxicity, through the  third
generation was observed however, the study is insufficient to evaluate the
reproductive toxicity of NAA. The number of animals bred was low and females
were bred to yield only one litter. Inadequate information was available  for
fertility, lactation and viability. No body weight data were available for the
pregnant dams, and no data were available for gestation. There were no
observation data for the pups; there were no data to indicate the number  of
pups found dead at birth. The data for pup body weight was inadequate and there
were no pathology data for the pups. In addition, the authors indicated  that no
toxicity was observed even at the highest doses.

While the above studies were performed with the methyl ester and do not  meet
with current scientific standards, the Agency has determined that additional
reproductive and chronic feeding testing with technical NAA will not be
required at this time, since these chamicals are minor use chemicals and
available data do not indicate major concerns in these areas.

Subchronic, chronic and mutagenicity toxicity testing with the  technical ethyl
ester of NAA is not required, since the subchronic, chronic  and mutagenic
toxicity of this chemical is expected to result  from exposure to  the  acid,
through ^n vivo metabolism.

     d. Acetamide

        1) Acute Effects

Because these are minor use chemicals no acute  testing with  technical
naphthaleneacetamide is required at this time.

        2) Subchronic/Chronic/Mutagenic Effects

Subchronic, chronic and mutagenicity toxicity testing  with the technical
naphthaleneacetamide is not required, since  the subchronic,  chronic and
mutagenic toxicity of this chemical is expected to  result from exposure  to the
acid, through ^n vivo metabolism.

  2. FORMULATED PRODUCTS

     a. Soluble Concentrates

      1) NAA

Because these are minor use chemicals,  except  for the primary eye irritation
study mentioned below, no acute  toxicity  testing will be required at this time,


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 Primary eye irritation testing with soluble concentrates containing greater
 than 50% petrolium oil or diacetone is required.

       2) Salts

           Sodium Salt

 Because these are minor use chemicals, no acute toxicity testing will be
 required at this time.

           Potassium Salt

 Because these are minor use chemicals, except for the primary eye irritation
 study mentioned below, no acute toxicity testing will be required at this time.

 Primary eye irritation testing with soluble concentrates containing the greater
 than 25% isopropanol is required.

           Ammonium Salt

 Because these are minor use chemicals no acute toxicity testing will be
 required at this time.

       3)  Ester

 An  adequate acute oral toxicity study in rats with a soluble concentrate
 containing 15.1% NAA-ethyl ester was available.  (Affiliated Medical Research,
 MRID GS-0023-035).  In this study, the test material (in water)  was given to
 three groups of male rats by oral intubation.  The LD5Q was determined to be
 5585 + 760 mg/kg.   Signs of toxicity included hypoactlvity in one rat at the
 highest dose (lOg/kg), from one hour after dosing until death.  Gross autopsy
 findings from the animals that died during the study included hemorrhagic
 stomach,  intestines and lungs, fluid and gas filled intestines and stomach,
 blanched  and slightly pitted kidneys, mottled spleen, bloody nose and stained
 genital area.   These data are sufficient to fulfill the acute oral toxicity
 data requirement for the 15.1% soluble concentrate of NAA-ethyl ester and place
 the  product in Toxicity Category IV.

 An  adequate acute dermal toxicity study in rabbits was available with a soluble
 concentrate containing 15.1% NAA-ethyl ester (Affiliated Medical Research, MRID
 GS-0023-032).   In  this study, the LD_Qwas determined to be greater than 5
 g/kg.   No  mortality was observed when four male albino rabbits were given 24
 hour dermal  exposure to 5 gAg of the product.   The test material was applied
 to the  shaved,  unabraded skin of the rabbits under an occlusive rubber wrap.
No signs of  toxicity were observed.   Gross pathological examination indicated
eschar  formation  at the site of application.  These data are sufficient to
 fulfill the  acute  dermal toxicity data requirement for the 15.1% soluble
concentrate  of NAA-ethyl ester and place this product in toxicity Category III.
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Adequate data were available to assess the acute  inhalation  toxicity of  a
soluble concentrate containing 15.1% NAA ethyl ester  in  rats.   (Affiliated
Medical Research, MRID GS-0023-034).  In this study,  the LC5Q was determined
to be greater than 217.1 mg/L.  No mortalities were observed* when 6  male albino
rats were given one hour inhalation exposure to a nominal concentration  of
217.1 mg/L of the soluble concentrate in a 50% w/v emulsion  with water.
Following exposure, signs of toxicity were irritation and lethargy.  The
animals appeared normal by 8-24 hours after exposure.  No abnormal gross
pathological alterations were observed.  These data are  sufficient to  fulfill
the acute inhalation toxicity data requirement for the 15.1% soluble
concentrate of NAA ethyl ester and place this product in Toxicity Category  IV.

the above data indicate a low acute toxicity potential for the  15.1% ethyl
ester in humans.
Adequate data were available to evaluate the primary  eye irritation  potential
of a soluble concentrate containing 15.1% of NAA-ethyl ester.   (Affiliated
Medical Research, MRID GS-0023-033).  In this study the  test chemical  was
determined to be a primary eye irritant.  When 0.1 ml of the test material  was
instilled into the left conjunctival sac of the eyes  of  nine male albino
rabbits (with six of the eyes being washed two to four seconds  after
instillation) corneal opacity that persisted for  at least seven days was
observed in eight out of nine eyes.  In addition, moderate to severe irritation
of the conjunctiva and iris persisted for at least seven days in some  animals.
At 14 days, damage that was described as irreversible by the pathologist was
observed.  These data are sufficient to evaluate  the  primary eye  irritation
potential of the 15.1% soluble concentrate of NAA-ethyl  ester and place  the
product in Toxicity Category I; indicating that the product  is  corrosive and
may cause irreversible eye damage in humans.

No data were available to evaluate the primary dermal irritation or  dermal
sensitization potential of products containing 15.1%  of  the  ethyl ester.
Testing is not required at this time.

      4) Acetamide

Because these are minor use chemicals, except for the primary eye  irritation
study mentioned below, no acute toxicity testing  will be required at this time.

Primary eye irritation testing with soluble concentrates containing  greater
than 50% isopropanol and greater than 50% fertilizer  is  required.

     b. Wettable Powders/Dusts

Because these are minor use chemicals no acute  toxicity  testing will be
required at this time.

     c. Pressurized Liquids

Because these are minor use chemicals no acute  toxicity  testing will be
required at this time.
                                  41

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B. Human and Domestic Animal Hazard Assessment

The present uses of these chemicals indicate that the actual  residues on  foods
would be extremely low. Actual  residues and theoretical  calculations of
residues, presented in the Environmental Pate chapter, confirm  that residues
will be extremely low.

Despite the fact that the method of application will result in  high exposure  to
the spray, the very low amounts of active  ingredient in  the spray will result
in a low exposure.
 Acute exposure of applicators  to the  concentrated  form  of these chemicals
during mixing/loading constitutes a scenario of concern  to the  Agency. Data
which demonstrate the acute toxicity category associated with the technical
chemicals are the Agency's primary concern and these concerns are reflected in
the Agency's data requirements.

C. Summary of Major Toxicology  Data Gaps

Many of the studies which have  been reviewed meet the Agency's  current
requirements. Some studies  (e.g., oncogenicity, reproduction) are  inadequate
but do not indicate major concerns in  these areas.  Because the  available  data
do not indicate major toxicological concerns and because these  are minor  use
chemicals with low dietary and  applicator  exposure, the  Agency  has determined
that no further testing, except for some acute and  irritation studies and a
teratogenicity study listed in  Chapter III, will be required at this time.
                                  42

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                        CHAPTER VII: RESIDUE CHEMISTRY

  A. Residue Chemistry Profile
  B. Summary of Data Gaps

A. Residue Chemistry Profile

Naphthaleneacetic acid is marketed in the U.S. as a plant growth regulator to
control fruit drop or thinning on apples, pears, quinces, and olives and to
control flower induction on pineapples. Tolerances have been established for
NAA on apples, pears, and quinces at 1 ppm; on olives at 0.1 ppm; and in or on
pineapples at 0.05 ppm (as the sodium salt) (40 CFR 180.155). Tolerances have
also been established for the ethyl ester on apples and pears at 1 ppn and 0.1
ppn on olives (40 CFR 180.309). Tolerances have also been established for the
acetamide on apples and pears at 0.1 ppn (40 CFR 180.309). No tolerances are
pending, fto CODEX tolerances exist for these chemicals.

The major residue in olives is NAA, the parent compound.  Metabolites in olives
are 1-naphthaleneacetylaspartic acid (NAAsp) and B-D-glucose-1-
naphthaleneacetate (NAG) (Crosby et al., MRID 000004943). The major residue in
apples and pears is B-D-glucose-1-naphthaleneacetate, followed by 1-
naphthaleneacetylaspartic acid, and then by NAA (Bukovac et al., MRID
005009196). Although no metabolism data are available on pineapples, data on
other fruits indicate that NAG, NAAsp, and NAA would be the residues of
concern, although not necessarily in that order. Metabolism data on apples can
be applied to quinces since these two fruits are related and quinces are a
minor crop use for these chemicals. The metabolism of NAA in plants is
adequately defined and no further testing is required. Metabolism data on
related compounds (i.e. the salts, ester, and amide of NAA) are not needed if
data showing rapid dissociation or hydrolysis to the acid are submitted.

The two major metabolites in rat urine are the conjugates of NAA with
glucuronic acid and with glycine (Lethco et al., MRID 000004942; French et al.,
MRID 005007966; Dixon et al., MRID 005008052; Dixon, MRID 005008057).
Metabolites in excrement of the hen are conjugates of NAA with taurine and
ornithine (Idle et al., MRID 005008070). The major and minor metabolites in
human urine are 1-napthylacetylglucuronide and 1-naphthylacetyl taurine,
respectively (Dixon ct al., MRID 005008052). No ruminant metabolism  study  is
available. It is concluded that the metabolism of NAA in animals is adequately
defined, provided residues do not occur in feed items.

Adequate analytical methods are available for determination of residues and
enforcement of tolerances for 1-naphthaleneacetic acid on olives (Crosby et
al., MRID 000004943; Zwaig et al., MRID 000004916), for the ethyl ester on
olives, and for 1-naphthaleneacetamide on apples (Sigrist, MRID 005007967;
Cochrane et al., MRID 005020083), pears and olives. Adequate analytical methods
are available for the determination of NAA itself on apples  (Randall, MRID
005002768; Cochrane et al.f MRID 005008121; Bache et al.r MRID 005008068),
pears, and pineapple (Young et al., MRID 000004946), and  for the ethyl  ester
itself (plus NAA) on pears (Anchem Products, MRID GS-0023-012;  Anchem Products,
MRID GS-0023-019; Amchem Products, MRID GS-0023-036) and  apples. No analytical
methods are available for quinces. Available residue data indicate  that


                                 43

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residues in olives would be  less  than 0.1 ppm after treatment with Fruit Fix
Super Concentration 800. No  detectable residues are expected to occur in
apples, pears, and olives  after treatment with Tre-Hold Sprout Inhibitor A112.
Lack of residue data on representativeformulations and uses constitutes a data
gap. The limited data which  are available indicate that no residues will result
in meat, milk, poultry, or eggs from the  registered uses.  There are no records
in EPA or FDA of incidents involving the  enforcement of the tolerances.

B. Summary of Data Gaps

  1. Dissociation or hydrolysis data on all  chemicals.

  2. Data indicating whether residues in  plants of esters, salts and
     acetarnides of NAA degrade rapidly to NAA.

  3. Additional residue data reflecting the  registered uses for apples and
     pears representative  of the  chemical forms of NAA and registered
     formulations.

  4. Residue data on pineapples and  olives are requested which reflect
     currently registered  uses and formulations.

If the data required in 1  and 2 indicate  that the various  chemical forms of NAA
are similar from a residue standpoint, then  the additional residue data
required in 3 and 4 in this  summary  can be kept to a minimum with specific
study requests.
                                 44

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                       CHAPTER VIII: ECOLOGICAL EFFECTS

  A. Plant Effects Profile
  B. Animal Effects Profile
  C. Summary of Data Gaps

A. Plant Effects Profile

The effects of NAA to nontarget aquatic plants is not known.  NAA at 620 ppb
caused a 50 percent reduction in root growth of soaked pea roots. There was
only a 10 percent reduction in root growth when NAA was applied to the
hypocotyl or young stem of peas at 500 ppm. (Elliasson, MRID 005007254) NAA
reduces growth in cucumber seedlings with a NOEL of 98 ppb (Hilton & Nomura,
MRID 005007255). The germination of lettuce, tomato, sugarbeet or dwarf bean
was unaffected in their yields when the seeds were treated with 100,000 ppm NAA
applied in talc (Croxall & Ogilvie, MRID 005012526). Concentrations of 0.1 ppm
in a seed soak, however, delayed or inhibited germination of cotton.  NAA at
100 ppm inhibited germination of cotton for 10-14 days without affecting yields
(Coats, MRID 005010399).

The concentration of NAA in the spray solution ranges from 10 to 50 ppm for
registered fruit crops and also for decidious trees.  The extent of exposure  to
nontarget terrestrial plants is minimal given the low concentration and,
therefore, the hazard is judged to be minimal.

B. Animal Effects Profile

Naphthalene acetic acid  (NAA acid) possesses a very low toxicity to Bobwhite
Quail when taken as a single oral dose. The acute oral LD^for Bobwhite Quail
is greater than 2510 mg/kg (Truslow Farms, MRID GS-0023-024). The acute oral
        Mallard Ducks cannot be determined because the use of  immature test
      s has compromised the validity of this study  (Truslow  Farms, MRID  GS-
0023-023).

Naphthalene acetic acid  (NAA acid) possesses a very low toxicity to Bobwhite
Quail and Mallard Ducks, when taken ingested along with their  regular  diet. The
subacute oral LC^for Bobwhite Quail and Mallard Ducks are both greater  than
10,000ppm (Truslow Farms, MRID GS-0023-025; Truslow Farms, MRID GS-0023-026).

While the LCSOvalues for the two freshwater fish and the  aquatic invertebrate
show that a formulated product, Amchem Tre-Hold Sprout Inhibitor A112,  is
slightly toxic to aquatic life  (Union Carbide Environmental  Services,  MRID GS-
0023-022; Union Carbide Environmental Services, MRID GS-0023-050; Union  Carbide
Environmental Services, MRID GS-0023-051).
                                 45

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C. Summary of Major Data Gaps

The Agency cannot presently estimate the hazards to aquatic life posed by the
technical chemicals because the proper toxicity tests have not been performed
on technical NAA. Before a hazard evaluation can be completed, the Agency must
have proper data on the acute toxicity of technical NAA to warmwater and
coldwater fish, an aquatic invertebrate, aquatic and terrestrial macrophytes,
and algae.
                                 46

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                            CASE BIBLIOGRAPHY

                       Guide to Use of This Bibliography


    Content £f Bibliography.  This bibliography contains citations of all the
    studies reviewed by EPA in arriving at the positions and conclusions stated
    elsewhere in this standard.  The bibliography is divided into two sections:
    (1)  citations in numerical order that contributed information useful to
    the review of the chemical and are considered to be part of the data base
    supporting registrations under the standard, and (2) an alphabetical
    listing of all documents reviewed by the Agency.  Primary sources for
    studies in this bibliography have been the body of data submitted to EPA
    and its predecessor agencies in support of past regulatory decisions, and
    the published technical literature.
2.  Units of Entry.  The unit of entry in this bibliography is called a
    "study".  In the case of published materials, this corresponds closely to
    an article.  In the case of unpublished materials submitted to the Agency,
    the Agency has sought to identify documents at a level parallel to a
    published article from within the typically larger volumes in which they
    were submitted.  The resulting "studies" generally have a distinct title
    (or at least a single subject), can stand alone for purposes of review,  and
    can be described with a conventional bibliographic citation.  The Agency
    has attempted also to unite basic documents and commentaries upon them,
    treating them as a single study.

3.  Identification of Entries.  The entries in this bibliography are sorted
    by author, date of the document, and title.  Each entry bears, to the left
    of the citation proper, a nine-digit numeric identifier.  This number is
    unique to the citations and should be used at any time specific reference
    is required.  This number is called the "Master Record Identifier" or
    "MRID".  It is not related to the six-digit "Accession Number", which has
    been used to identify volumes of submitted data; see paragraph 4(d)(4)
    below for a further explanation.  In a few cases, entries added to the
    bibliography late in the review may be preceded by a nine-character
    temporary identifier.  This is also to be used whenever a specific
    reference is needed.

4.  Form of the Entry.  In addition to the Master Record Identifier  (MRID),
    each entry consists of a bibliographic citation containing standard
    elements followed, in the case of materials submitted to EPA, by a
    description of the earliest known submission.  The bibliographic
    conventions used reflect the standards of the American National Standards
    Institute (ANSI), expanded to provide for certain special needs.  Some
    explanatory notes of specific elements follow:

    a.   Author.  Whenever the Agency could confidently  identify one,  the
         Agency has chosen to show a personal author.  When no  individual was
         identified, the Agency has shown an identifiable laboratory or  testing
         facility as author.  As a last resort, the Agency has  shown the first
         known submitter as author.
                                 47

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b.   Document Date.  When the date appears  as  four digits with no
     question marks, the Agency took  it directly  from  the document.  When  a
     four-digit date is followed by a question mark, the bibliographer
     deduced the date from evidence in the  document.   When  the date appears
     as  (19??), the Agency was unable to determine or  estimate the date of
     the document.

c.   Title.  This is the third element in the  citation.  In some cases it
     has been necessary for Agency bibliographers to create or enhance a
     document title.  Any such editorial insertions are contained between
     square brackets.

d.   Trailing Parentheses.  For studies submitted to us in  the past, the
     trailing parentheses include  (in addition to any  self-explanatory
     text) the following elements describing the  earliest known
     submissions:

     (1)  Submission Date.  Immediately following the  word  'received1
          appears the date of the earliest  known  submission, at the time
          that particular document was processed  into  the Pesticide
          Document Management System.

     (2)  Administrative Number.  The next  element, immediately following
          the word 'under1, is the registration number, experimental permit
          number, petition number, or other administrative  number
          associated with the earliest known submission, at the time that
          particular document was processed into  the Pesticide Document
          Management System.

     (3)  Submitter.  The third element is  the submitter, following the
          phrase 'submitted by1.  When authorship is defaulted to the
          submitter, this element is  omitted.

     (4)  Volume Identification.  The final element in the  trailing
          parenthesis identifies the  EPA accession number of the volume in
          which the original submission of  the study appears.  The six-
          digit accession number follows the symbol 'CDL1,  standing for
          "Company Data Library".  This accession number is in turn
          followed by an alphabetic suffix which  shows the  relative
          position of the study within the volume.  For example, within
          accession number 123456, the first study would be 123456-A; the
          second, 123456-B;  the 26th, 123456-Z; and the 27th,123456-AA.
                             48

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                         OFFICE OF PESTICIDE PROGRAMS
                 REGISTRATION STANDARD NUMERICAL BIBLIOGRAPHY
          Citations Considered to be Part of the Data Base Supporting
                       Registrations Under the Standard
CASE   GS0023   Napthaleneacetic Acid

MRID        CITATION

000004916   Zweig, G.; Gutnick, D.L.; Gulli, R.; Archer, T.E.; Hartmann, H.T.
                (1962) Residue Determination of Naphthaleneacetic acid in
               Olives.  Undated method.  (Unpublished study received Jan 25,
               1963 under 642-149; prepared by Univ. of California—Davis,
               Dept. of Pomology, and Agricultural Toxicology and Residue
               Research Laboratory, submitted by Thompson Chemicals Corp.,
               Carson, Calif.; CDL:119788-B)

000004942   Lethco, E.J.; Brother, E.A. (1966) The metabolism of naphthalenea-
               cetic acid-l-C  in rats.  Journal of Agriculture and Food
               Chemistry 14(5):532-535.  (Also ^n unpublished submission re-
               ceived Nov 25, 1970 under 1E1099; submitted by Interregional
               Research Project No. 4, New Brunswick, N.J.; CDL:090859-A)

000004943   Crosby, D.G.; Bowers, J.B.; Shindy, W.W. (1970) 1-Naphthaleneacetic
               acid in Olives.  Includes two undated methods.  (Unpublished
               study received Nov 25, 1970 under 1E1099; prepared by University
               of California at Davis, Dept. of Environmental Toxicology and
               Agricultural Experiment Station, submitted by Interregional
               Research Project No. 4, New Brunswick, N.J.; CDL:090859-B)

000004946   Young, H.Y.; Shimabukuro, S.; Aono, L.  (1963) Spectrophotometric
               microdetermination of 1-Naphthaleneacetic acid in pineapple.
               Agricultural and Food Chemistry 11(2):132-133.  (Pineapple
               Research Institute of Hawaii, Technical paper no. 284; also  In
               unpublished submission received Nov 25, 1970 under 1E1099; suE-
               mitted by Interregional Research Project No. 4, New Brunswick,
               N.J.; CDL:090859-F)

005002768   Randall, R.C. (1970) UV determination of naphthaleneacetic  acid  in
               apples and potatoes.  Journal of the Association of Official
               Analytical Chemists 53(1):149-151.

005003760   Bache, C.A.; Llsk, D.J.; Loos, M.A.  (1964) Electron affinity
               residue determination of nitrated MCP, MCPB, and NAA; conversion
               of MCPB to MCP in bean plants.  Journal of the Association of
               Official Agricultural Chemists 47(2):348-352.

005005277   Loveless, L.E.; Spoerl, E.; Weisman, T.H.  (1954) A survey of
               effects of chemicals on division and growth of yeast  and
               Escherichia.coli .  Journal of Bacteriology 68:637-644.

005007254   Ellasson, L. (1961) Responses of pea roots to growth substances.
               Physiologia Plantarum 14:803-812.

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 005007255   Hilton, H.W.; Nomura, N. (1964) Phytotoxicity of herbicides as
                measured by root absorption.  Weed Research 4(3):216-222.

 005007264   Migrdichian, V., inventor;  American Cyanamid Co., assignee (1943)
                Method of refining crude 1-naphthylacetic acids.  U.S. patent
                2,336,364.  Dec 7.  3 p. Cl. 260-525.

 005007284   Southwick, P.L., inventor;  Pood Machinery and Chemical Corp.,
                assignee (1953)  Method of making naphthaleneacetic acid.  U.S.
                patent 2,655,531.  Oct 13.  2 p. Cl. 260-515.

 005007966   French, M.R.; Bababunmi, E.A.; Golding, R.R.; Bassir, 0.; Caldwell,
                J,; Smith, R.L.; Williams, R.T. (1974) The conjugation of phenol,
                benzoic acid, 1-naphthylacetic acid and sulphadimethoxine in the
                lion, civet and genet.  FEES Letters 46(1):134-137.

 005007967   Sigrist, R.; Temperli, A.;  Hurter, J. (1974) A fluorometric method
                for the determination of residues of 1-naphthaleneacetamide and
                1   ttithaleneacetic acid on apples.  Journal of Agricultural
                anu food Chemistry 22(4):568-570.

 005008052   Dixon, P.A.F.;  Caldwell, J.; Smith, R.L. (1977) Metabolism of
                arylacetic acids: 1: The fate of 1-naphthylacetic acid and its
                variation with species and dose.  Xenobiotica 7(11):695-706.

 005008057   Dixon, P.A.F.;  Caldwell, J.; Woods, C.J.; Smith, R.L. (1976)
                Influence of chemical structure on the pattern of conjugation ^in
                vivo and in vitro of some arylacetic acids in the rat.
                Biochemical  Society Transactions 4(1):143-145.

 005008068   Bache, C.A.; Bdgerton, L.J.; Lisk, D.J. (1962) Determination of
                naphthaleneacetic acid in apples.  Journal of Agricultural and
                Food Chemistry 10(5):365-366.

 005008070   Idle,  J.R.; Millburn, P.; Williams, R.T.; Zini, G. (1976) The
                conjugation  of arylacetic acids in the pigeon compared with the
                hen.  Biochemical Society Transactions 4(1):141-143.

 005008106   Verschuuren, H.G.;  Kroes, R.; Tonkelaar, E.M. den; Esch, G.J. van;
                Helleman, P.W. (1976) Short-term toxicity of 1-naphthaleneacetic
                acid in rats. Toxicology 5(3):371-378.

 005008121   Cochrane,  W.P.;  Lanouette,  M. (1979)  High pressure liquid
                chromatographic  determination of naphthaleneacetic acid residues
                in  apples.   Journal of the Association of Official Analytical
                Chemists 62(1):100-106.

 005008826   Doi, S.; Takahashi, T.; Yanagishima, N. (1973) Auxin-induced large
                cell mutants in  Saccharomyces cerevisiae   I-  Induction, and
                biochemical  and  genetic  characters.   Japanese Journal of
               Genetics. A  translation  of:  Idengaku Zasshi. 48(3):185-195.

005009027   Gramlich,  J.V.;  Frans, R.E.  (1964)  Kinetics of Chlorella
                inhibition by herbicides.  Weeds 12(3):184-1871

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005009196   Bukovac, M.J.; Flore, J.A.; Goren, R. (1976)  Conjugation of foliar
               absorbed NAA by selected fruit crops.  Hort. Science 11(4):389-
               390

005010016   Bionetics Research Laboratories (1968) Evaluation of carcinogenic,
               teratogenic, and mutagenic activities of selected pesticide and
               industrial chemicals, vol I: carcinogenic study. Bethesda, Md.:
               National Cancer Institute, Division of Cancer Cause and
               Prevention. (NationalCancer Institute report no. NCI-OCCP-CG-
               1973-1-1; available from: NTIS, Springfield, VA; PB-223 159)

005010399   Coats, G.E. (1966) Growth Regulator Effects on Cottonseed
               Treatment.  State College, Miss.:  Mississippi State University,
               Agricultural Experiment Station. (Mississippi Agricultural
               Experiment Station information sheet 935)

005010797   Ukita, T.; Tamemasa, 0.; Motomatsu, H. (1951) Antibacterial action
               of fatty acids.  VIII-  Syntheses of fatty acids with naphthyl,
               naphthomethyl and benzyl groups in alpha-position and their
               antibacterial action.  Yakugaku Zasshi.  Journal of
               Pharmaceutical Society of Japan. 71(4):289-297.

005010966   Klein, D.T. (1962) Effect of growth-regulators on mutants of
               Neurospora.crassa.  Physiologia Plantarum 15:239-245.

005011538   Clifford, D.R.; Woodcock, D.  (1968) Fungal detoxication—IX:
               Metabolism of 1-naphthaleneacetic acid by Aspergillus niger Van
               Tiegh.  Phytochemistry 7(9):1499-1502.

005012526   Croxall, H.E.; Ogilvie, L.  (1940) The effect of seed dressings
               containing growth-promoting substances on lettuce, tomato, sugar
               beet and dwarf bean.  Pages 29-34, In Long Ashton Research
               Station, University of Bristol, Report 1940.  Bristol, England:
               Long Ashton Agricultural and Horticultural Research Station.

005020083   Cochrane, W.P.; Lanouette, M.; Grant, R.  (1980) High pressure
               liquid chromatographic determination of naphthaleneacetamide
               residues in apples.  Journal of the Association of Official
               Analytical Chemists 63(1):145-148.

005022160   Finch, N.  (1945) Effects on mice of a diet containing methyl  ester
               of alph naphthalene acetic acid. Contributions  from Boyce
               Thompson Insitute 14:69-78.

GS0023001   Huntington Research Center  (1976) Ten-day range-finding  study with
               NAA by gavage to rats.  (Amchem Products; CDL:009029, EPA Reg.
               No. 246-336; PPf OF2277)

GS0023002   Burchfield, H.P.; Johnson, D.E.  (1965) Guide to the analysis  of
               pesticide residues - volume I.  [U.S.Deparment of Health,
               Education and WeHrre, Public Health Service, Bureau of State
               Services (Environmental Health) Office of PPfsJ   (PPf  1E1099,
               pages 64 to 99)

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 GS0023012   Amchem Products (1978) Determination of residues of 1-
                naphthaleneacetic acid (NAA) and 1-naphthaleneacetic acid, ethyl
                ester (EtNAA) in olives. Apr, 1978 [Document f 478A3; PPI OF2277
                OF2277, CDL:099028; Sec D-3; pages 1 through 16.]

 GS0023019   Amchem Products (1978) Analysis of pear samples for possible
                residues of NAA and NAA ethyl ester. [PP! OF2277; CDL:099028;
                EPA Reg. Do. 264-336; Sec D-4.]

 GS0023022   Union Carbide Environmental Services (1979) The acute toxicity of
                72-A112 to rainbow trout Salmo gaidneri Richardson [Mar 2, 1979;
                USCE Project No. 11506-24-10] (Amchem Products; CDL:099029, EPA
                Reg. No. 264-336; PPf OF2277)

 GS0023023   Truslow Farms (1976) Acute oral LDen-mallard ducks [Project I 113-
                124; May 27, 1976] (Amchem Products; CDL:099029, EPA Reg. No.
                264-336; PPf OF2277)

 GS0023024   Truslow Farms (1976) Acute oral LD5Q-bobwhite quail [Project f
                113-153; May 30, 1976] (Amchem Products; CDL:099029, EPA Reg.
                No. 264-336;  PP! OF2277)

 GS0023025   Truslow Farms (1976) 8 Day dietary LCcn-bobwhite quail [Project f
                113-122; May 30, 1976] (Amchem Products; CDL:099029, EPA
                Reg. No. 264-336; PPf OF2277)

 GS0023026   Truslow Farms (1976) 8 Day dietary LC,.n-mallard ducks [Project t
                113-123; May 27, 1976] (Amchem Products; CDL:099029, EPA
                Reg. No. 264-336; PPI OF2277)

 GS0023027   Truslow Farms (1976) 8 Day dietary LCen-mallard ducks [Project I
                113-119; May 5,  1976]  (Amchem Products; CDL:099029, EPA
                Reg. No. 264-336; PP! OF2277)

 GS0023028   Truslow Farms (1976) 8 Day dietary LC«.n-bobwhite quail [Project f
                113-118; May 5,  1976]  (Amchem Products; CDL:099029, EPA
                Reg. No. 264-336; PP! OF2277)

 GS0023029   Truslow Farms (1976) Acute oral LDen-bobwhite quail [Project f
                113-154; May 30, 1976] (Amchem Products; CDL:099029, EPA Reg.
                No.  264-336;  PPf OF2277)

GS0023031    Elars  Bioresearch Laboratories (1979) Six  month oral toxicity study
                of  naphthalene acetic acid in beagle dogs. [Amchem Products;
                CDLj  099035;  Project f 1395]

GS0023032    Affiliated  Medical  Research  (19*7)  Acute dermal LD.Q in
                rabbits  (formulated  product). [Contract! 120-2148-113; Undated]
                (Amchem  Products;  CDL:099029, EPA Reg.  No. 264- 336; PPf OF2277)

GS0023033   Affiliated  Medical  Research  (197?)  Primary eye irritation of Amchem
                72-A112  emulsifiable  concentrate 79068. [Contract!  120-2148-113;
                Undated]  (Amchem Products; CDL:099029,  EPA Reg.  No. 264-336; PP!
                OF2277)

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GS0023034   Affiliated Medical Research  (19??) Acute inhalation of Amchem
               72-A112 emulsifiable concentrate in rats. (Contract* 120-2148-
               113? Undatedl (Amchem Products; CDL:099029, EPA Reg. No. 264-
               336; PP* OF2277)

GS0023035   Affiliated Medical Research  (19??) Acute oral in rats (formulated
               product). [Contract* 120-2148-113; Undated] (Amchem Products;
               CDL:099029, EPA Reg. No. 264- 336; PP* OF2277)

GS0023036   Amchem Products  (1978) Analysis of apple samples for possible
               residues of NAA and NAA methyl ester. [PP* OF2277; CDL:099028;
               EPA Reg. No. 264-336; Sec D-5]

GS0023037   Pharmakon Laboratories (1978) Yeast ( Saccharomyces cerevisiae  )
               Strain D-7 mitotic crossing over assay on: 1-naphthalene acetic
               acid, Lot GN-2095  [Amchem Products; Jul 17, 1978; CDL:
               099029, EPA Reg. No. 264-336; PPf OF2277]

GS0023038   Pharmakon Laboratories (1978) Yeast ( Saccharomyces cerevisiae  )
               Strain D-7 reverse assay on: 1-naphthalene acetic acid, Lot GN-
               2095 [Amchem Products; Jul 17, 1978; CDL:099029, EPA Reg. No.
               264-336; PP# OF2277]

GS0023039   Pharmakon Laboratories (1978) Yeast ( Saccharomyces cerevisiae  )
               Strain D-7 mitotic gene conversion assay on: 1-naphthalene
               acetic acid, Lot GN-2095  [Amchem Products; Jul 17, 1978; CDL:
               099029, EPA Reg. No. 264-336; PP* OF22771

GS0023040   Pharmakon Laboratories (1978)  Eschericia coli  DNA polymerase  I
               deficient  assay on: 1-naphthalene acetic acid, Lot GN-2095
               336; PP* OF2277]

GS0023041   Pharmakon Laboratories (1978) Ames Salmonella/microsomal  plates
               test (with and without metabolic activation) on: 1-naphthalene
               acetic acid, Lot GN-2095  fAmchem Products; May 11, 1978; CDL:
               099029, EPA Reg. No. 264-336; PP# OF2277]

GS0023042   Pharmakon Laboratories (1979) Perform the micronucleus test
               according to s.o.p. ph 309 on: 1-naphthalene acetic acid, Lot GN-
               2095 [Amchem Products; Jan 30, 1979; CDL:099029, EPA Reg. No.
               264-336; PP* OF2277]

GS0023043   Pharmakon Laboratories (1979) Dominant lethal study  (1-
               naphthalene acetic acid - 16338)  [Amchem Products; Feb 28,
               1979; CDL:099029, EPA Reg. No. 264-336; PP* OF2277]

GS0023044   Huntington Research Center  (1977) Teratology study with NAA acid
               (technical) by gavage in  the albino rat.  [Study * R-4216-4  (1-
               350); Jan 14, 1977] (Amchem Products; CDL:099029, EPA  Reg. No.
               264-336; PP* OF2277)

GS0023045   Affiliated Medical Research  (197?) Primary eye  irritation of  tech
               ethyl ester of NAA. [Contract* 120-2148-113; Undated]  (Amchem
               Products; CDL:099029, EPA Reg. No. 264- 336; PP* OF2277)

-------
GS0023046
GS0023047
GS0023048
GS0023049
Affiliated Medical Research  (19??) Acute inhalation of tech
   ethyl ester of NAA in rats. [Contract! 120-2148-113; Undated]
   (Amchem Products; CDL:099029, EPA Reg. No. 264- 336; PPf OP2277)

Affiliated Medical Research  (19??) Acute dermal LD-Q in
   rabbits (technical chemical).  [Contract! 120-2158-113; Undated!
   (Amchem Products; CDL:099029, EPA Reg. No. 264- 336; PP! OF2277)

Affiliated Medical Research  (19??) Acute oral IA-0 in
   rats (technical chemical). [Contract! 120-2148-113; Undated]
   (Amchem Products; CDL:099029, EPA Reg. No. 264- 336; PP! OF2277)

Truslow Farms (1976) Acute oral LD50-mallard ducks [Project ! 113-
   155; Mar 6, 1976] (Amchem Products; CDL:099029, EPA Reg. No.
   264-336; PP! OF2277)
GS0023050   Union Carbide Environmental Services  (1979) The acute  toxicity of
               72-A112 to the water  flea Daphnia  magna Stratus  [Apr 20,  1979;
               USCE Project No. 11506-24-11]  (Amchem Products; CDL:099029, EPA
               Reg. No. 264-336; PP! OF2277)

GS0023051   Union Carbide Environmental Services  (1979) The acute  toxicity of
               72^A112 to the bluegill sunfish Lepomis machochirus Rafinesque
               CDL:099029, EPA Reg. No. 264-336;  PP! OF22T7}

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                          OFFICE OF PESTICIDE PROGRAMS
                REGISTRATION STANDARD ALPHABETICAL BIBLIOGRAPHY
         Listing of All Documents Identified in the Literature Search
CASE   GS0023   Naphthaleneacetic Acid

MIRD        CITATION

005018584   Aaron, J.J.; Kaleel, E.M.; Winefordner, J.D. (1979) Comparative
               study of low-temperature and room-temperature phosphorescence
               characteristics of several pesticides.  Journal of Agricultural
               and Food Chemistry 27(6):1233-1237.

005018580   Aaron, J.J.; Winefordner, J.D. (1979) Heavy atom effect on the
               room-temperature phosphorimetry of some aromatic pesticides.
               Analusis 7(4):168-171.

005009921   Abdel-Rahman, M.; Isenberg, F.M.R.  (1974) Effects of growth
               regulators and controlled atmosphere on stored carrots.
               Journal of Agricultural Science 82(2):245-249.

005015450   Abraham, P.D.; Boatman, S.G.; Blackman, G.E.; Powell, R.G. (1968)
               Effects of plant growth regulators and other compounds on flow
               of latex in Hevea brasiliensis .  Annals of Applied Biology
               62(1): 159-171:

005017649   Abueva, A.A. (1965) Deistvie razlichnykh gerbitsidov na tomaty  i
               zarazikhu-  The effect of various herbicides on tomatoes and
               broom rape.  Izvestiya Timiryazevskoi Sel'skokhozyaistvennoi
               Akademii.  Bulletin of the Timiryazev Agricultural Academy.
               (4):156-165.

GS0023032   Affiliated Medical Research (19??) Acute dermal LD-Q in
               rabbits (formulated product).  [Contract* 120-2ll8-113; Undated!
               (Amchem Products? CDL:099029f EPA Reg. No. 264- 336; PP* OF2277)

GS0023047   Affiliated Medical Research (19??) Acute dermal LD,Q in
               rabbits (technical chemical).  [Contract! 120-2148-113; Undated]
               (Amchem Products; CDL:099029, EPA Reg. No. 264- 336; PP* OF2277)

GS0023034   Affiliated Medical Research (19??) Acute inhalation of Amchem
               72-A112 emulsifiable concentrate  in rats. [Contract* 120-2148-
               113; Undated] (Amchem Products; CDL:099029, EPA Reg. No. 264-
               336; PP* OF2277)

GS0023046   Affiliated Medical Research (19??) Acute inhalation of tech
               ethyl ester of NAA in rats. [Contract* 120-2148-113; Undated]
               (Amchem Products; CDL:099029, EPA Reg. No. 264- 336; PP* OF2277)
                                                   r
GS0023048   Affiliated Medical Research (19??) Acute oral LDu0 in
               rats (technical chemical).  [Contract* 120-2148-113;  undated]
               (Amchem Products; CDL:099029, EPA Reg. No.  264- 336;  PP* OF2277)

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 GS0023035   Affiliated Medical Research (19??) Acute oral in rats (formulated
                product). [Contract* 120-2148-113;  Undated]  (Amchem Products;
                CDL:099029, EPA Reg. Mo. 264- 336;  PP# OF2277)

 GS0023033   Affiliated Medical Research (19??) Primary eye irritation of Amchem
                72-A112 emulsifiable concentrate 79068. [Contract* 120-2148-113;
                Undated!  (Amchem Products;  CDL:099029, EPA Reg. No. 264-336;  PP#
                OF2277)

 GS0023045   Affiliated Medical Research (19??) Primary eye irritation of tech
                ethyl ester of NAA.  [Contract* 120-2148-113;  Undated]  (Amchem
                Products; CDL:099029, EPA Reg. No.  264- 336;  PP# OP2277)

 005009195   Agnihotri, J.P.;  Prasad, N. (1966) Effect of growth regulating
                substances on growth of Colletotrichum
                capsici F- Cyamopsicola Prasad.  Labdev 4(4):260-261.

 005012105   Akai,  S. (1955)  Chemotherapeutic application of  some compounds to
                rice  plants and the  outbreak of Helminthosporium leaf  spot.
                Shokubutsu Byogai Kenkyu.   Plant Diseases Research.]  5:45-56.

 005007064   Allen, H.P.; Brian, R.C.; Downes, J.E.;  Mees, G.C.; Springett,
                R.H.  (1978) Selective herbicides.  Pages 35-41, In Jealott's
                Hill: 50  Years of Agricultural Research 1928-1978.  Edited by
                F.C.  Peacock.   Bracknell, Berks, England:  Jealott's Hill
                Research  Station.

 005016580   Alleweldt, G.; Hifny, H.A.A. (1972) Zur  Stiellaehme der Reben: II.
                Kausalanalytische Untersuchungen.  On the stiellaehme  of  the
                grape vine: II-  Investigations on  the causes of the disease
                Vitis 11(1): 10-28.

 005012283   Ambroz,  Z.  (1963)  Prispevek k  poznani  proteolyzy v pude.   Notes
                on  the proteolysis in the soil.  Rostlinna Vyroba.  Plant
                Production. 9(7/8):802-806.

 GS0023036   Amchem Products (1978)  Analysis of apple samples for possible
                residues  of NAA and  NAA methyl ester. [PP# OF2277; CDL:099028;
                EPA Reg.  No. 264-336;  Sec D-5]

GS0023019   Amchem Products (1978)  Analysis of pear  samples  for possible
                residues  of NAA and  NAA ethyl ester.  [PP# OF2277; CDL:099028;
                EPA Reg.  No. 264-336;  Sec D-4.]

GS0023012    Amchem Products  (1978)  Determination of  residues of 1-
                naphthaleneacetic acid (NAA)  and 1-naphthaleneacetic acid, ethyl
                ester (EtNAA)  in olives.  Apr,  1978  [Document  #  478A3;  PP* OF2277
                OF2277, CDL:099028;  Sec D-3;  pages  1  through  16.]

000004925   Amchem Products,  Incorporated  (1952)   Chemical Thinning:  The Influ-
                ence  of Timing  and Concentration on Mature Appl6 Trees].   (Un-
                published study received Jan 29,  1960 under 264-137; submitted
               by  Union Carbide Agricultural  Products Co., Ambler, Pa.;
               CDL:002080-O)

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000004937
000004924
000004936
000004938
000004929
000004926
000004945
000004920
005008063
005002575
005002576
Amchem Products, Incorporated (1954) Apple Set Records.  (Unpub-
   lished study received Jan 29, 1960 under 264-137;  submitted by
   Union Carbide Agricultural Products Co., Ambler, Pa.; CDL:
   002080-K)

Amchem Products, Incorporated (1954?)  Results of Chemical Thinning
   of Several Varieties of Apple Trees].  (Unpublished study re-
   ceived Jan 29, 1960 under 264-137; submitted by Union Carbide
   Agricultural Products Co., Ambler, Pa.; CDL:002080-C)

Amchem Products, Incorporated (1955) Set Records--! 955.  (Unpub-
   lished study received Jan 29, I960 under 264-137;  submitted by
   Union Carbide Agricultural Products Co., Ambler, Pa.; CDL:
Amchem Products, Incorporated (1955) York, Tbnoloway Orchard, Han-
   cock— 1955.  (Unpublished study received Jan 29, 1960 under 264-
   137; submitted by Union Carbide Agricultural Products Co., Am-
   bler, Pa.; CDL:002080-L)

Amchem Products, Incorporated (1957) Some Results in Chemical Thin-
   ning Apples— 1952 to 1955.  (Unpublished study received Jan 29,
   1960 under 264-137; prepared in cooperation with Univ. of Mary-
   land, Dept. of Horticulture, submitted by Union Carbide Agricul-
   tural Products Co., Ambler, Pa.; CDL:002080-H)

Amchem Products, Incorporated (1958) Chemical Thinning — 1957.   (Un-
   published study received Jan 29, 1960 under 264-137; prepared  in
   cooperation with Pennsylvania State Univ., Agricultural and Home
   Economics Extension Service, submitted by Union Carbide Agricul-
   tural Products Co., Ambler, Pa.; CDL:002080-E)

Amersham/Searle Corporation  (1968) Radiochemical Data.   (Unpub-
   lished study received Nov 25, 1970 under 1E1099; submitted by
   Interregional Research Project No. 4, New Brunswick, N.J.; CDL:
   090859-E)

Anderson, H.H.; Shimkin, M.B.; Leake, C.D. (1936)  Acute  intra-
   peritoneal toxicity of some plant growth substances for mice.
   Page 138, In Proceedings of Society for Experimental Biology and
   Medicine; Jan 25, 1936, Pomona College.   The Society.
   (Abstract only; also In unpublished submission received Mar 18,
   1968 under 239-1884; submitted by Chevron Chemical Co.,
   Richmond, Calif.; CDL: 124527-B)

Anon.  (1968) Legislation: United States, United Kingdom,  European
   Economic Community, Canada, France, Italy, Japan, Netherlands,
   Norway, Venezuela, West Germany.  Pood and Cosmetics Toxicology
   6 (6): 761-777.

Anon.  (1968) Legislation-  United States-  Amendments  to  Title  21,
   Chapter 1 of the Code of Federal Regulations.  Food and
   Cosmetics Toxicology 6 (6): 761-767.

Anon.  (1968) U.S. pesticide production again tops billion pound
   level.  Agricultural Chemicals 23 (12): 36-37.

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 005004172


 005009043



 005007911



 005011993




 005016110
 Anon. (1973)  Common names of pesticides.
    1973.   PANS 19 (2):287-306.
Revised list—February
 000004934
 005008068
000004953
005003760
000004915
005009970
 Archer,  T.E.  (1966)  Preparation and identification of
    2-chloroethyl 1-naphthyl acetate.  Bulletin of Environmental
    Contamination and Toxicology 1(4):172-174.

 Armarego,  W.L.F.; Canny,  M.J.;  Cox, S.F.  (1959)  Metal-chelating
    properties of plant-growth substances.   Nature
    183 (4669):1176-1177.

 Ashton,  F.M.;  Penner, D.; Hoffman,  S.  (1968) Effect of several
    herbicides on proteolytic activity of squash  seedlings.   Weed
    Science 16 (2):169-171.

 Bache, C.A. (1967) Naphthalene  acetic acid. Pages
    455-467, ^n Additional Principles and Methods of Analysis.
    Edited  by G. Zweig and J. Sherma.  New  York:   Academic Press.
    (Analytical  methods for pesticides, plant growth regulators,
    and food additives,  vol.  5)

 Bache, C.A.;  Edgerton,  L.J.; Lisk,  D.J.  (1961) Determination of
    Naphthaleneacetic acid in Apple.  Includes  undated method.   (Un-
    published  study received Nov 27, 1961 under 264-142;  prepared by
    Cornell  Univ., submitted by  Union Carbide Agricultural Products
    Co.,  Ambler, Pa.; CDL:002117-B)

 Bache, C.A.;  Edgerton,  L.J.;  Lisk,  D.J.  (1962) Determination of
    naphthaleneacetic acid in apples.  Journal  of Agricultural  and
    Food  Chemistry 10(5):365-366.

 Bache, C.A.;  Edgerton,  L.J.;  Lisk,  D.J.  (19??) Determination of
    Naphthaleneacetic acid in apples.   Agricultural  and Food  Chem-
    istry ?(?):365-366.  (Also In unpublished submission received
    Feb 17,  1971 under IE1094; submitted by Interregional Research
    Project  No.  4, New Brunswick, N.J.; CDL:093406-F)

 Bache, C.A.; Lisk, D.J.;  Loos,  M.A. (1964)  Electron affinity
    residue  determination  of  nitrated MCP,  MCPB,  and NAA; conversion
    of MCPB  to MCP in bean plants.   Journal  of  the Association  of
    Official Agricultural  Chemists 47(2):348-352.

 Baker, D.  (1963)  Evaluation  for MR  Use of  Alpha-Naphthalene  acetic
    acid  on  Olives Submitted  by  Thompson Chemicals:  9-4-63.   Summary
   of study 119788-B.   (Unpublished study  received  Jun 25, 1963
   under 642-149; submitted  by  Thompson Chemicals Corp., Carson,
   Calif.; CDL:119788-A)

Bamberger, E.S.;  Sharabani, M.  (1969) The  effect  of plant growth
   regulators on DNA.  Israel Journal of Chemistry  7:122.

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005019382   Bana, D.S.; Srivastava, R.P.;  Om, H. (1976)  Studies on the
               thinning of the fruits by hand and with plant growth substances
               and their effects on the final retention and quality of apple
               cv. Red Delicious.  Indian Journal of Horticulture
               33 (3/4): 227-232.

005018620   Banwell, M.G. (1973) The role of herbicides in modern fruit
               management.  Pages 1002-1011, In Proceedings of the llth
               British Weed Control Conference; Nov 13-16, 1972, Brighton,
               England.  London, England:   British Crop Protection Council.

005015470   Barlow, H.W.B. (1947) Intensive experimental methods in abscission
               studies.  Pages 121-125, In Annual* Report, East Mailing
               Research Station, Kent.  Maidstone, England:  East Mailing
               Research Station.

005009104   Barlow, H.W.B.; Dicker, G.H.L.; Briggs, J.B. (1955) Studies on
               control of apple sawfly, Hoplocampa testudinea (Klug).  II.
               Effect of sprays on fruit drop and yield.Pages
               107-114, In East Mailing Research Station Annual Report, 1954.
               Maidstone, England:  East Mailing Research Station.

005011969   Baskakov, Y.A.j Mel'nikov, N.N. (1955) O poluchenii
               alpha-naftiluksusnoi kisloty po reaktsii Vil'gerodta.
               reaction.]  Zhurnal Prikladnoi Khimii (Leningrad).   Journal of
               Applied Chemistry.1 28:1016-1018.

005011539   Beachy, R.N.; Murakishi, H.H. (1971) Local lesion formation in
               tobacco tissue culture.  Phytopathology 61(7):877-878.

005020176   Beck, D.L.; Sosebee, R.E.; Herndon, E.B. (1975) Control of honey
               mesquite by shredding and spraying.  Journal of Range
               Management 28 (6):487-490.

005011859   Beerh, O.P.; Krishnamurthy, G.V.; Narasimham, P.; Giridhar, N.;
               Raghuramaiah, B. (1976) Effect of pre- and post-harvest
               treatments to control some common disorders in Anab-e-Shahi
               grapes ( Vitis vinifera Linn).  Journal of Food Science and
               Technology, India 13(3):129-132.

005014945   Bernhard, K.; Caflisch-Weill, H. (1949) Das Schicksal der
               Decahydro-naphtylessigsaeuren und der Naphtylessigsaeuren  im
               Tierkoerper.   The fate of decahydro-naphthylacetic  acids  and
               naphthaleneacetic acids in the animal body.]  Helvetica Chimica
               Acta XXXII(III):625-631.

005008076   Bhatt, J.G.; Verma, S.S.  (1958) Effect of growth regulating
               substances on virus affected tomato plants.  Science and
               Culture 23(11):610-611.

005015356   Bhattacharyya, R.; Sharma, A.K. (1976) Differential action of
               hormones in inducing abnormalities in chromosome behaviour and
               cell growth.  Pages 332-347, In Recent Advances  in Botany.
               Professor P.N. Mehra Commemorative Volume.  Edited by P.   ,
               Kachroo.  Dehra Dun, India:  Bishen Singh  Mahendra Pal Singh.

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 005019041    Bilkey,  P.C.;  McCown,  B.H.  (1979)  In vitro  culture  and  propagation
                of Eplscia  sp.  (flame violets).   Journal of the  American
                Society for Horticultural  Science 104 (1):109-114.

 005010016    Bionetics  Research Laboratories  (1958)  Evaluation of  carcinogenic,
                teratogenic,  and mutagenic activities of selected  pesticide  and
                industrial  chemicals, vol  I:  carcinogenic study. Bethesda, Md.:
                National  Cancer Institute, Division  of Cancer Cause  and
                Prevention. (NationalCancer Institute report no. NCI-DCCP-CG-
                1973.1-1; available from:  NTIS,  Springfield, VA; PB-223 159)

 005008014    Blackman,  G.E. (1945)  Recent  developments in chemical methods for
                the selective control of weeds.   Journal of the  Royal
                Agricultural  Society of England  106:137-150.

 005018821    Blanpied,  G.D.;  Forshey, C.G.; Styles,  W.C.;  Green, D.W.; Lord,
                W.J.; Bramlage, W.J.  (1975) Use  of ethephon to stimulate  red
                color without hastening ripening of  "Mclntosh" apples.  Journal
                of the  American Society for Horticultural Science
                100 (4):379-381.

 005019042    Blazich, F.A.; Heuser, C.W. (1979)  A histological study of
                adventitious  root initiation  in  mung bean cuttings.  Journal of
                the American  Society for Horticultural Science 104(1):63-67.

 005020384    Blazich, F.A.; Heuser,  C.W. (1979)  The  mung bean rooting bioassay:
                a  re-examination.   Journal of the American Society for
                Horticultural Science 104 (1):117-120.

 005015704    Borkowski, J.  (1973) The influence  of NAA-sprays on flowering and
                earliness of  fieldcucumber.   Acta Horticulture  27:249-252.

 005019569    Boswell, S.B.; Bergh,  B.O.; Whitsell, R.H.; Kumamoto, J.  (1979)
                Reduction of  rootstock sprouts on young  grafted  avocados  with
                NAA.  HortScience 14(1):57-59.

 005017239    Bourke, J.B.;  Butts, J.S.; Fang, S.C. (1964)  Effect of  various
                herbicides  on glucose metabolism in  root tissue  of garden peas.
                II.  Plant  growth regulators  and other herbicides.  Weeds
                12 (4): 272-276.

005015706    Bowers, J.B.;  Crosby,  D.G. (1971) Determination of
                1-naphthaleneacetic acid in olives.  Abstracts of  Papers,
               American Chemical Society  161:77.

005009745    Brenner, M.L.  (1969) Mechanism of naphthaleneacetic acid
                accumulation  by soybean ( Glycine max Merr.) leaf  cells with
               special emphasis on tissue preparation and viability.
               Dissertation  Abstracts International B 31(5):2399-2400.

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005009108   Brenner, M.L.; Tonkinson, T.R.C. (1974) Extraction and
               identification of L-N-(d-napthyl)  acetyl]  glutamic acid, a
               major product of naphthaleneacetic acid metabolism.  Pages
               759-764, In Mechanisms of Regulation of Plant Growth.  Edited
               by R.L. Bieleski, A.R. Ferguson and M.M. Cresswell.
               Wellington, New Zealand:  Royal Society of New Zealand. (Royal
               Society of New Zealand bulletin no. 12; Minnesota Agricultural
               Experiment Station scientific journal series paper no. 8409)

005013069   Brill, K.E. (1972) Effect of ultraviolet radiation on
               auxin-induced elongation and the mechanical properties of
               Alaska pea stem segments.  Dissertation Abstracts International
               B 32 (11):6211-6212.

005010175   Buggeln, R.G. (1976) Auxin, an endogenous regulator of growth in
               algae  Journal of Phycology 12(3):355-358.

000004963   Bukovac, M.J. (1962) A Summary of Results on the Effect of Naph-
               thylacetamide for Fruit Thinning of Pears.  (Unpublished study
               including letter dated Dec 18, 1962 from M.J. Bukovac to Mel
               Suterland, received Dec 4, 1970 under 1E1094; prepared by
               Michigan State Univ., Dept. of Horticulture, submitted by Inter-
               regional Research Project No. 4, New Brunswick, N.J.; CDL:
               090854-M)

000009625   Bukovac, M.J. (1963) Apples: Chemical promotion of flowering.
               American Fruit Grower 83(3):24.  (Also ^n unpublished submission
               received Jul 24, 1969 under 264-184; submitted by Union Carbide
               Agricultural Products Co., Ambler, Pa.; CDL:002156-C)

000004962   Bukovac, M.J-. (1963) Chemical Thinning of Pears with Naphthylacet-
               amide: Summary of Results Obtained During the 1963 Growing
               Season.  (Unpublished study including letter dated Apr 9, 1964
               from M.J. Bukovac to E.A. Walker, received Dec 4, 1970 under
               1E1094; prepared by Michigan State Univ., Dept. of Horticulture,
               submitted by Interregional Research Project No. 4, New
               Brunswick, N.J.; CDL:090854-L)

005018575   Bukovac, M.J.; Flore, J.A.; Baker, E.A.  (1979) Peach leaf
               surfaces: changes in wettability, retention, cuticular
               permeability, and epicuticular wax chemistry during expansion
               with special reference to spray application.  Journal of the
               American Society for Horticultural Science 104(5):611-617.

005009196   Bukovac, M.J.; Flore, J.A.; Goren, R.  (1976) Conjugation of foliar
               absorbed NAA by selected fruit, crops.  Hort. Science 11 (4):389-
               390

005010625   Bukovac, M.J.; Norris, R.F. (1966) Foliar penetration of plant
               growth substances with special reference to binding by
               cuticular surfaces of pear leaves.  Agrochimica 10:296-309.

005008582   Bukovac, M.J.; Norris, R.F. (1968) Foliar penetration of plant
               growth substances with special reference to binding by
               cuticular surfaces of pear leaves.  Agrochimica
               XII(2/3):217-230.

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 GS0023021   Burchfield, H.P.; Johnson, D.E. (1965) Guide to the analysis of
                pesticide residues - volume I.  [U.S.Deparment of Health,
                Education and Welfare, Public Health Service, Bureau of State
                Services (Environmental Health) Office of PPls]   (PP# 1E1099,
                pages 64 to 99)

 005008062   Caldwell, J.;  French, M.R.; Idle,  J.R.; Renwick, A.G.; Bassir, 0.;
                Williams, R.T., (1975)  Conjugation of foreign compounds in the
                elephant and hyaena.  FEBS Letters 60(2):391-395.

 000004917   California Department of Agriculture, Division of Chemistry (1966)
                Report To Sender on Information Sample as Received:  Pear
                Samples.  (Unpublished study including undated letter from E.P.
                Delfino to William Wade, received Aug 8,  1969 under 279-2131;
                submitted by FMC Corp., Philadelphia, Pa.; CDL:002443-A)

 005008120   Campbell, R.A.; Durzan, D.J. (1975)  Induction of multiple buds and
                needles in tissue cultures of Picea glauca  .  Canadian Journal
                of Botany 53 (16):1652-1657.

 005008812   earlier, A.; Van Assche, J. (1968) Estimation of respiration
                pathways, including corrections for glucuronic acid
                decarboxylation and label randomization;  effect of
                1-naphthy]acetic acid and cobalt chloride.  Zeitschrift fuer
                Pflanzenphysiologie 59 (4):353-363.

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005014518   Charykov, A.K.; Talnikova, T.V. (1974) pH-Metricheskii metod
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005019968   Chen, W.S.; Uemoto, S. (1977) Sosai no calcium-kyushu nikansuru
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 005008058   Coggins, C.W., Jr.;  Jolliffe, V.A.;  Shindy,  W.W.;  Knapp,  J.C.F.
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005008053   Coleman, W.K.; Thorpe, T.A.  (1978)  In vitro  culture of western red
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005009496


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005010518   Flerov, A.F.;  Kovalenko, E.I. (1952) Vliyanie alpha-naftiluksusnoi
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005009197   Flore, J.A.; Bukovac, M.J. (1976) EPTC-induced changes in
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005009003   Fonnesbech, M. (1974) The influence of NAA, BA and temperature  on
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005009001   Foster, C.A. (1969) Efficacy of selective gametocides on Lolium
                perenne L.  Annals of Botany 33(133):947-950.

005015703   Franke, G.; El-Hamid Hassanein, A.E.  (1976) 7um Einfluss von GS,
               CCC, MH und NES auf Keimung und Jugendentwicklung von Zea
                mays L. bei unterschiedlicher NaCl-Substratversalzung.    On
               the effect of GS, CCC, MH and NES on the germination and
               initial development of Zea mays L. at different NaCl substrate
               salting.]  Beitraege zur Tropischen Landwirtschaft und
               Veterinaermedizin 14(4):361-367.

00501507*2   Franzia, A.P.; Menendez, R.A. (1978) Water sprout control  in  apple
               cultivars.  Compact Fruit Tree 11:120-124.

005007966   French, M.R.;  Bababunmi, E.A.; Golding, R.R.; Bassir, 0.;  Caldwell,
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               benzole acid, 1-naphthylacetic acid and sulphadimethoxine  in the
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005015071   French, R.C.;  Beevers, H.  (1953) Respiratory and growth  responses
               induced by growth regulators and allied compounds.   American
               Journal of Botany 40:660-666.

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 000009495   Gambrell, C.E., Jr.; Rhodes, W.H.j Sims, E.T., Jr. (1967) Some Ef-
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                turity of Ranger Peaches When Applied for One Year and Two  Y^ars
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 000009494   Gambrell, C.E., Jr.; Rhodes, W.H.; Suber, R.D., Jr. (1965) The Ef-
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                of Ranger Peaches and Their Effect on Vegetative Growth of  Trees
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 005008083   Georgieva, M.;  Vellnov, G.; Budevsky, 0. (1978) Acid-base
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                the conditions for tltration  of some aromatic carboxylic acids
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005016578    Gondo,  M.  (1953)  Shokubutsu hormone no tabako mozaiku byosho
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005015357   Gondo, M.  (1954)  Shokubutsu hormone no  tabaco mosaic
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Gorter, C.J. (1957) Abscission as a bio-assay for the
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Gorter, C.J. (1960) Oplosbaarheid van groelstoffen.   Solubility
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Gortner, W.A. (1952) Water of crystallization In the plant-growth
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Gortner, W.A.; Leeper, R.W. (1969) Studies on the relation of
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Gourley, L.M.; Keim, W.F.; Stob, M. (1969) Influence of
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Cowing, D.P.; Leeper, R.W.  (1960) Studies on the relation of
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Cowing, D.P.; Leeper, R.W.  (1960) Studies on the relation of
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Goza, S.W. (1972) Infrared analysis of pesticide formulations.
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Grace, N.H. (1937) Physiological curve of response  to  phyto hor-
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Gramlich, J.V.; Frans, R.E. (1964) Kinetics of  Chlorella
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 005016240   Grashuis, J.; Man, T.J. de (1945) Over de mogelijke beinvloeding
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 005008711   Greene, D.W. (1969) Factors influencing the foliar penetration of
                naphthaleneacetic acid and naphthaleneacetamide into leaves of
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                International B 30(12):5384-5385.

 005009028   Greene, D.W.; Bukovac, M.J. (1972) Penetration of
                naphthaleneacetic acid into pear ( Pyrus communis L.)  leaves.
                Plant and Cell Physiology 13(2):321-336.

 005010171   Greene, D.W.; Bukovac, M.J. (1977) Foliar penetration of
                naphthaleneacetic acid:  enhancement by light and role of
                stomata.   American Journal of Botany 64(1):96-101.

 005019084   Greene, D.W.; Lord, W.J.; Bramlage, W.J.; Southwick, F.W. (1974)
                Effects of low ethephon concentrations on quality of "Mclntosh"
                apples.  Journal of the  American Society for Horticultural
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 005019936   Greene, D.W.; Lord, W.J.; Southwick, F.W.; Bramlage, W.J. (1973)
                Effects of SADH (Alar),  ethephon and preharvest drop control
                chemicals on quality of  Mclntosh apples.  Pages 112-119, In New
                England Fruit Meeting: Proceedings of the Annual Meeting cT the
                Massachusetts Fruit Growers' Association.  Vol. 79.  North
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 005012928   Griehl, W. (1947) Ueber eine  neue Methode zur Herstellung von
                alpha-Naphthylessigsaeure.   A new method for the preparation
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 005019385   Grochowska,  M.J.; Lubinska, A.  (1973)  Comparative studies on
                physiological and morphological features of bearing and
                non-bearing  spurs of the apple tree: II.  The effect of
                fruiting  and growth  regulator  sprays on respiration rate of
                leaves.  Physiologia Plantarum 29(3):425-429.

 005018473    Gubbels, G.H. (1979)  Yield  and weight  per seed in buckwheat after
                foliar  applications  of growth  regulators and antitranspirants.
                Canadian  Journal  of  Plant  Science 59 (3):857-859.

005007766   Gunther, F.A.,  ed. (1971) Residue Reviews.  Vol. 35.
                Residues  of  Pesticides and Other Foreign Chemicals in Foods
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005015639   Haccius, B.  (1955) Versuche zur somatischen Beeinflussung der
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               XI(4):149-152.

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005007775



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005019571


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005013616



005020235



005008903
005019578
005008532


005005070



005007255


005018828
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Hallock, H.C.; Deen, O.T. (1956)  Greenhouse  tests  on  control  of
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Hammerton, J.L. (1975)  Experiments with Cyperus rotundus L.t  II.
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Handley, L.W.; Chambliss, O.L. (1979)  In vitro  propagation
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Hardison, J.R. (1977) Chemical control of ergot in field plots
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Barney, P.M.; Knap, A. (1979) A technique for the  in  vitro
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Harries, F.H. (1966) Reproduction and  mortality of the two-spotted
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Harrison, M.A.; Klein, R.M. (1979) Role of growth  regulators in
   initiation of secondary xylem and phloem cells.  Botanical
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Hasman, M.; Inane, N. (1958) Investigations upon the mechanism of
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Heng, D.A. (1974) Physiological role of succinic
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Herregods, M. (1971) Storage of tomatoes.
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Hilton, H.W.; Nomura, N.  (1964) Phytotoxicity of herbicides as
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Himelrick, D.G.; Pollard, J.E.; Estes, G.O.  (1976) Effect of
   daminozide and NAA on Ca uptake and accumulation in "Mclntosh"
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Hoffman, M.B.; Edgerton, I. (1951) Results of Commercial Orchard
   Tests with ACP: Amid Thin, New York State, 1951.   (Unpublished
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 000004941
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 Holdsworth, R.P.  (1973)  Realities of  integrated insect control.
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 Hopf,  P.P.; Hey,  G.L. , inventors; Ward Blenkinsop &  Co.,  assignee
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 Huntington  Research Center (1977)  Teratology  study with  NAA acid
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 Idle, J.R.; Millburn, P.;  Williams, R.T.  (1976) Taurine
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Idle, J.R.; Millburn, P.; Williams, R.T.;  Zini, G.  (1976) The
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Ireland,  D.S.;  Brown, J.R. (1977) Novel tetracycline analogs.
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Jerebzoff-Quintin, S. (1963)  Les substances secretees par Rhizopus
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Jolliffe, V.A.; Coggins, C.W., Jr. (1970) A fluorometric method
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Joshi, R.C.; Shukla, T.C. (1974) Effect of hormones on  germination
   of Phaseolus aureus RoxB.   PKV Research Journal 3(1):80-81.

Joshi, R.D.; Dubey, L.N. (1975) Studies on the inhibition of
   cucumber mosaic virus in chilli ( Capsicum annuum L.): III.
   Effect of growth regulators.  Science and Culture
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Kamada, H.; Harada, H. (1979) Influence of several growth
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Kanazawa, J.; Masuda, T.; lizuka, H.; Yamada, T.; Suzuki, T.
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Kato, Y. (1978) Induction of adventitious buds on undetached
   leaves, excised leaves and leaf fragments of Heloniopsis
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Kaveriappa, K.M.; Safeeulla,  K.M.  (1975) Nuclear behaviour  and
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Kazemie, M.; Klaembt, D.  (1969) Untersuchungen zur Aufnahme von
   Naphthalin-1-essigsaeure  und ihres Asparaginsaeure-Konjugates
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   naphthalene-1-acetic acid  and  its aspartic  acid conjugate  into
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Khan, S.U.  (1975) Chemical derivatization  of herbicide  residues
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   New York:  Springer.

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 005010966   Klein, D.T. (1962) Effect of growth-regulators on mutants of
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 005015903   Kosinski,  J.;  Nikonorow, M.  (1959) Spektrofotometryczne ilosciowe
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 005015499   Kovina, I.M.;  Tsukervanik, I.P.  (1964) Karboxyalkilirovanie
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 005006184   Kozinka, V. (1967) Water uptake during rapid changes of
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 005008904   Kozinka, V. (1967) Water uptake  during rapid changes of
                transpiration induced by the  presence of high concentration of
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 005005839   Kozinka, V. (1970) Inhibition of water uptake by high
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 005008578   Kraus,  E.J.; Mitchell,  J.W. (1947) Growth-regulating substances as
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 005019959   Krause, R.T.  (1979)  Resolution,  sensitivity and selectivity of a
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005018676   Kroeller, E. (1974)  Eine  Moeglichkeit rvc Restmengenbestimmung von
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005003123   Kulkarni, A.P.; Mailman,  R.B.?  Hodgson,  E.  (1975) Cytochrome P-450
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005014523   Kuraishi, S. (1977) Shokubutsu seicho choseizai  no  genjo to
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005008067   Kutsky, R.J.; Rawlins, T.E. (1950)  Inhibition of virus
               multiplication by naphthalene acetic acid in  tobacco  tissue
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005010968   LaBrecque, G.C.; Adcock,  P.H.;  Smith, C.N.  (1960) Tests  with
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005010627   LaRue, J.H.; Sibbett, G.S.; Bailey, M.S.;  Fitch, L.B.; Yeager,
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005015497   Laibach, P.; Kribben, F.I. (1950) Der Einfluss  von VAjchsstoff auf
               die Bildung maennlicher und weiblicher  Blueten bei einer
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005014528   Laibach, F.; Kribben, F.J. (1950) Der Einfluss von Wuchsstoff auf
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 005011543   Norris, R.F.; Bukovac, M.J.  (1969)  Some  physical-kinetic
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 005009038   Norris, R.F.; Bukovac, M.J.  (1972)  Influence  of cuticular waxes on
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 005017901   Notten, W.R.F.; Henderson, P.T. (1977) The interaction of chemical
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GS0023040   Pharmakon  Laboratories (1978)  Eschericia coli  DMA polymerase I
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                336;  PPf OF2277]

GS0023041   Pharmakon  Laboratories (1978) Ames Salmonella/microsomal plates
                test (with and without metabolic activation) on:  1-naphthalene
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GS0023039   Pharmakon Laboratories (1978) Yeast ( Saccharotnyces cerevisiae )
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GS0023037
GS0023038
GS0023043
GS0023042
005007272


000004960
005015070


005018836



005013428


005015503
005015371
005002768
Pharmakon Laboratories (1978)  Yeast (  Saccharomyces cerevisiae )
   Strain D-7 mitotic crossing over assay on:  1-naphthalene acetic
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                                       •

Pharmakon Laboratories (1978)  Yeast (  Saccharomyces cerevisiae )
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Pharmakon Laboratories (1979)  Dominant lethal  study (1-
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Pharmakon Laboratories (1979)  Perform the micronucleus  test
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 005015358   Ravazzoni, C.  (1951) Azione di sostanze di crescita ed erbicidi
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 005013264   Ray, P.M.; Dohrmann, U.; Hertel, R. (1977) Characterization of
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 005010075   Ray, P.M.; Dohrmann, U.; Hertel, R. (1977) Specificity of
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005013864   Roblinr R.O.,  Jr.;  Hechenbleikner,  I.,  inventors;  American
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005008092   Rock, G.C.; Yeargan,  D.R. (1973)  Toxicity of  apple orchard
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005015086   Ross, M.; Salisbury,  F.B. (1962)  The  effect of herbicides on high
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000009447   Rubatzky, V.E.; Sciaroni, R.H.  (1970) Chemical Topping of Brussels
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005014529   Ruge, U. (1978) Anwendungsmoeglichkeiten von Wachstumsregulatoren
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005008103   Sanders, L.B.; Winefordner,  J.D. (1972) Phosphorescence
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005007283   Scherr, G.H.,  inventor;    (1966)  Anti-microbial potentiatlon with
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               May 17.  8  p.  Cl.  167-65.

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 005010628   Schneider, G.W.  (1973) Effects of NAA and Sevin on the structure
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 005018034   Schneider, G.W.  (1977) Studies on the mechanism of fruit
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 005009046   Schneider, G.W.; Lasheen, A.M. (1973) NAA and Sevin on
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 005013425   Schumacher, R.;  Fankhauser, P.;  Stadler, W. (1977)
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 005007285   Schwenk,  E.;  Papa,  D., inventors; Schering Corp., assignee (1950)
                Alpha-beta-substituted aliphatic acids and their salts and
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005007976    Scott, P.C.; Norris,  L.A. (1970)  Separation of effects of auxin
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005019220    Sedgley,  M.  (1979)  Ovule  and seed growth in pollinated and
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                Botany (London)  43 (2):135-140.

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005012279
005009200   Sen Gupta, P.K.;  Das,  C.R.  (1964)  Effect of vitamins,  hormones,
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005007091   Shetiya, R.S.; Rao, K.N.; Shankar, J. (1972) Determination  of rate
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005014866   Shiga, N.; Matano, 0.; Goto, S. (1976)  l^Naphthaleneacetic  acid no
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005007570   Shindy, W.W.; Jordan,  L.S.; Jolliffe, V.A.; Coggins, C.W.,  Jr.;
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005008090   Shindy, W.W.; Jordan,  L.S.; Jolliffe, V.A.; Coggins, C.W.,  Jr.;
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005009035   Shindy, W.W.; Lyon, J.L.; Gauer, W.O.;  Crosby, D.G.; Addicott,
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005007967   Sigrist, R.; Temperli, A.;  Hurter, J.  (1974) A  fluorometric  method
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005008298   Simard, A. (1971) Initiation of DMA synthesis by  kinetin and
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005011549   Simola, L.K.; Sopanen, T.  (1971) Effect of  alpha-naphthalene- and
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 005005399   Stahl, E. (1969)  TAS-Verfahren zur schnellen Abtrennung von
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 005020381   Tajima, K.; Shimizu, N.  (1977) Effect of membrane stabilizers and
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Truslow Farms (1976) Acute oral LD50-bobwhite quail  [Project I
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Truslow Farms (1976) Acute oral LDcn'bobwhite quail  [Project f
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Truslow Farms (1976) Acute oral LDcn-mallard ducks  [Project f  113-
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Truslow Farms (1976) Acute oral LD50-mallard ducks  [Project f  113-
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005012164   Wu, M.T.; Singh, B.; Theurer, J.C.; Olson, L.E.;  Salunkhe, O.K.
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005008906   Yadava, R.B.R.;  Pachauri, V.C.; Rekib, A.  (1978)  Nutritive value
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005014980   Yanagishima, N.; Shimoda, C. (1967) Production of yeast variants
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005013756   Yoshida, K.; Nishizaki, Y.  (1976) Sushosuiseidobutsu  ni  taisuru
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 005007281   Zellner, H.,  inventor; Danube-Pharmazie G.m.b.H., assignee (1964)
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005008814    Zweig, G.; Archer, T.E.; Raz, D.  (1962) Residue determination of
               naphthaleneacetic acid and its  methyl  ester  in potatoes by a
               combination of gas chromatography  and  ultraviolet
               spectrophotometry.  Journal of  Agricultural  and Food  Chemistry
               10 (3):199-203.

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000004916   Zweig, G.;  Gutnick, D.L.; Gulli, R.;  Archer, T.E.; Hartmann,  H.'
                (1962?)  Residue Determination of Naphthaleneacetic acid  in
                Olives.   Undated method.   (Unpublished study received Jan
                1963  under 642-149; prepared by Univ.  of California—Dav,a,
                Dept. of Pomology, and Agricultural  Toxicology and Resid ie
                Research Laboratory, submitted  by  Thompson Chemicals Corp.,
                Carson,  Calif.; CDL:119788-B)
                                                             *OA GOVERNMENT PRINTING OFFICE:  1911  341-082/250
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