United States " SR-101B
Environmental Protection
Agency .
Research and
Development
MDEX AND ABSTRACTS TO PUBLICATIONS
THE EPA-NCI PROJECT: EFFECTS OF
CARCINOGENS, MUTAOENS, AND
TERATOGENS ON NON- HUMAN SPECIES
(AQUATIC ANIMALS)
Prepared by
Environmental Research
Laboratory
Gulf Breeze FL 32561
FEBRUARY. .1984
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THIS PUBLICATION
THE NATIONAL CANCER INS1ITUT6 (NCI) FUNDED AN EPA STUDY ENTITLED:
"EFFECTS OF CARCINCGENS, MHAGENS AND TERATQGENS ON NON-HUMAN SPECIES
(ACUATIC ANIMALS)" FROM 1978 TO 1982. ASSOCIATED WITH THIS EFFORT WERE
OR. H. KRAYBILL (NCI) AND CR. JOHN CCUCH CEPA)f PRINCIPAL INVESTIGATOR
AND PROJECT COORDINATOR.
TO DATE, THE JOINT EFFORT HAS PRODUCED ABOUT 100 REPORTS, SYMPOSIA
SECTIONS OR PUBLICATIONS IN THE PEER-REVIEWED, JOURNAL LITERATURE.
CONTRIBUTIONS ARE LISTED, hITH ABSTRACTS (WHEN AVAILABLE) AND CROSS
REFERENCED BY TITLE-KEY WORDS (SEE PAG€ 56). ADDITIONALLY, AN AUTHOR
INDEX IS PROVIDED (SEE PAGE 42).
QUESTIONS CONCERNING THE PROJECT SHCULO 8E ADDRESSED TO OR. J. COUCH
(CCMMERCIAL 904-932-5311 OF FTS 686-9011). REPRINT OR REPORT REQUESTS
SHCULO 8E DIRECTED TO MS. AND8EE LOWRY (COMMERCIAL 904-932-5311 OR
FTS 686-9011). THIS PUBLICATION SUPERSEDES SR-103 AND SR-101A,
HENRY F. ENDS
LABORATORY DIRECTOR
PREPARATION DATE:
FEBRUARY, 1984
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TABLE OF CONTENTS PAGE
ABOUT THIS PUBLICATION i
TITLES AND ABSTRACTS 1
KEYWORD TITLE INDEX 41
AUTHOR INDEX 56
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ANDERSON, ROBERT S. 1978. B£NZO(A)PYRENE METABOLISM IN THE AMERICAN OYSTER
CRASSOSTRSA VIRGINICA. EPA-600/3-78-009, U.S. ENVIRONMENTAL PROTECTION
AGENCY, ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, FL. 19P.
THIS RESEARCH PROGRAM WAS INITIATED WITH THE OVERALL OBJECTIVE OF
DETERMINING THE ROLE OF NADPH-DEPENOENT MICROSOMAL MONQ-OXYGENASE IN
THE METABOLISM OF THE WIDESPREAD ENVIRONMENTAL CARCINOGEN
BENZO BY THE OYSTER CRASSOSTREA VIRGINICA. THIS ENZYME
SYSTEM IS IMPORTANT IN DETOXIFYING VARIOUS XSNOBIOTICS AND IN
ACTIVATING POLYCYCLIC AROMATIC HYDROCARBON ONCOGENS AS BP. A SENSITIVE
RAOIOISOTOPIC SYSTEM WAS DEVELOPED TO PERMIT THE QUANTIFICATION OF
ALKALI-SOLUBLE AND WATER-SOLUBLE BP METABOLITES PRODUCED BY OYSTER
MONO-OXYGENASE. AN NADPH- AND OXYGEN-DEPENDENT ARYL HYDROCARBON
HVDROXYLASE CAMH> WAS SHOWN TO BE LOCATED IN THE DIGESTIVE GLANDS OF
THESE BIVALVES ASSOCIATED WITH THE MICROSOMAL SU8CELLULAR FRACTION.
THE SPECIFIC ACTIVITY OF OYSTER AHH WAS CONSIDERABLY LOWER THAN THAT
OF LABORATORY MICE, BUT WAS CONSISTENTLY DEMONSTRABLE. THE BP
METABOLITES PRODUCED WERE PRIMARILY WATER-SOLUBLE DERIVATIVES. THERE
WAS SOME INDICATION THAT OYSTER AHH WAS INDUCED BY CHRONIC EXPOSURE OF
THE ANIMALS TO THE ENVIRONMENTAL CARCINOGENS BP AND
3-METHYL-CHOLANTHRENE. THERE WAS STRONG EVIDENCE THAT EXPOSURE TO
POLYCHLORINATEO 6IPHENYLS (PC3) CAUSED AHH INDUCTION. HIGH-PRESSURE
LIQUID CHROMATOGRAPHY WAS USED TO IDENTIFY BP METABOLITES PRODUCED BY
OYSTER AHH. THE GENERATION OF VARIOUS DIHYDRODIOL, QUINONE, AND
HVOROXY BP DERIVATIVES WAS SHOWN; THIS PRODUCTION WAS AUGMENTED IN
PCB-EXPOSED OYSTERS. THIS REPORT COVERS THE PERIOD FROM JULY 1, 1976
TO JUNE 30, 1977, AND WORK WAS COMPLETED AS OF AUGUST 31, 1977.
ANDERSON, ROBERT S., JESSICA E. ODDS, AND FRANCIS L. ROSE. 1982.
DIFFERENTIAL ABILITY OF AMBYSTOMA TIGRINUM HEPATIC NICROSOMSS FROM POLYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES. CANCER LETT. 160)133-42.
.
A NUMBER OF CARCINOGENIC AROMATIC AMINES, WHEN ACTIVATED BY LIVER
MXCROSOMES FROM A SALAMANDER, AMBYSTOMA TIGRINUM, ARE MUTAGENIC FOR
SALMONELLA TESTER STRAINS SENSITIVE TO FRAMESHIFT MUTAGENS. HOWEVER, 2
POLYCYCLIC AROMATIC HYDROCARBONS CPAH) CBENZOCA3PYRENE €6Af>> AND
PERYLENE) THAT ARE RENDERED MUTAGENIC BY MAMMALIAN MICROSOMES ARE NOT
ACTIVATED BY AMBYSTOMA MIXED-FUNCTION QXIOASES. BAP WAS CHOSEN FOR
STUDY BECAUSE IT IS A WELL-KNOWN ENVIRONMENTAL CARCINOGEN! PERYLENE,
AN ISOMER OF BAP, HAS BEEN IMPLICATED AS AN ETIOLOGICAL AGENT IN
CUTANEOUS NEOPLASIA IN AMBYSTOMA. THESE RESULTS SUPPORT THE
OBSERVATION THAT AMPHIBIANS ARE QUITE RESISTANT TO PAH CARCIN06ENESIS
AND SUGGEST THAT AROMATIC AMINES MAY BE MORE APPROPRIATE MODEL
CARCINOGENS.
PAGE
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BIBA, DIANE MAR*. 1983. EFFECTS OF AFLATQXIN ON THE BROWN BULtMEAO
ICTALURUS NE6ULOSIS. M.S. THESIS. AUBURN UNIVERSITY, AUBURN, AL. 53P,
(ERLtGB X357).
BROUN BULLHEADS HAD RENAL LESIONS 25 DAYS AFTER A ONE-HOUR EXPOSURE TO
1.0 MGG/LITER AFLATOXIN 8-1 CAFB) DURING THE FIFTH DAY OF EMBRYONIC
DEVELOPMENT, INDICATING A SIMILARITY TO THE EFFECTS OF AFB REPORTED
FOR RAINBOW TROUT. RENAL LSSIONS WERE NOT FOUND IN BROUN BULLHEADS
AFTER EMBRYO EXPOSURE TO 0.5 MG/LITER AFB. LIVER HISTOLOGY AND
ULTRASTRUCTURE OF CONTROL BROWN BULLHEADS WERE SIMILAR TO CHANNEL
CATFISH, ICTALURUS PUNCTATUS. NO LESIONS WERE OBSERVED IN BROWN
BULLHEAD LIVERS EXAMINED 25 DAYS TO EIGHT MONTHS AFTER EMBRYO EXPOSURE
TO 0.5 MG/LITER OR 1.0 MG/LITER AFB. THE LACK OF LIVER TUMOR INDUCTION
EIGHT MONTHS AFTER EMBRYO EXPOSURE TO 0.5 MG/LITER AFB OR 1.0 MG/LITER
AFB SUGGESTS THAT LONGER INDUCTION TIMES OR HIGHER DOSAGES OF AFB ARE
REQUIRED FOR TUMORIGENICITY IN BROWN BULLHEADS. A BENZCK A)PYRENE PYRENE METABOLISM IN AN
OYSTER COSTREA EDULIS)-8ACTERIA SYSTEM. M.S. THESIS. OREGON STATE
UNIVERSITY, CORVALLIS, OR. 121P. (ERL,GB X285).
THE OVERALL OBJECTIVE OF THIS RESEARCH PROJECT WAS TO EVALUATE THE
METABOLISM OF BENZOCA>PYRENE BY THE EUROPEAN FLAT OYSTER COSTREA
EOULIS>-BACTERIA SYSTEM. INDIVIDUAL OYSTERS EXIST IN NATURE AS
"SYSTEMS* WITH COMMENSAL MICROOGRANISMS AND SHOULD BE RECOGNIZED AS
SUCH WHEN THE METABOLISM OF A SPECIFIC ENVIRONMENTAL CONTAMINANT IS
BEING STUDIED. SINCE BIVALVES ARE EXPOSED TO ENVIRONMENTAL POLLUTANTS
THAT ENTER COASTAL WATERS, IT IS IMPORTANT TO DETERMINE IF THE
"NATURAL" OYSTER SYSTEM CAN DETOXIFY THOSE CONTAMINANTS LIKELY TO
CAUSE BIOLOGICAL DAMAGE.
PAGE
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COUCH. J.A., J.T. WINSTEAQ, D.J. HANSEN, AND L.R. GOODMAN. 1979. VERTEBRAL
OYSPLASIA IN YOUNG FISH EXPOSED TO THE HER8ICIOE TRIFLURALIN. J. FISH
DISEASES. 2
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COUCH, JOHN A. 1979. SHRIMPS
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COUCHt JOHN A., LEE A, COURTNEY, AND STEVEN S. FOSS. 1981. LABORATORY
EVALUATION OF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS. IN: PHYLETIC
APPROACHES TO CANCER: PROCEEDINGS OF THE 11TH INTERNATIONAL SYMPOSIUM OF THE
PRINCESS TAKAMATSU CANCER RESEARCH FUND, TOKYO, 1980. CLYDE J. DAWE, EDITOR,
JAPAN SCIENTIFIC SCIENCES PRESS, TOKYO, JAPAN. PP. 125-139. CERL,GB 171>.
THE U.S. EPA AND THE NATIONAL CANCER INST. CNCI) HAVE MAJOR
RESPONSIBILITIES FOR DETERMINING THE FATE AND RISKS OF CARCINOGENIC
AGENTS IN THE NATURAL ENVIRONMENT. UNDER THE AUSPICES OF EPA/NCI, THE
CARCINOGEN RESEARCH TEAM AT THE USEPA LAS., GULF BREEZE, HAS A MAJOR
ROLE IN INVESTIGATING THE FATE, EFFECTS, AND RISKS OF CARCINOGENIC
AGENTS IN THE AQUATIC PORTION OF THE BIOSPHERE. IN REGARD TO THIS
ROLE, THERE IS A NEED FOR PRACTICAL, EXPERIMENTAL EXPOSURE SYSTEMS FOR
THE SHORT TERM, AND LONG TERM EXPOSURE OF FISHES AND INVERTEBRATES IN
ORDER TO EVALUATE THEIR RESPONSES TO ENVIRONMENTALLY SIGNIFICANT
CARCINOGENS. WE HAVE DESIGNED AND TESTED AN ADAPTABLE, AQUATIC
LABORATORY SYSTEM FOR FLOWING WATER OR STATIC WATER ASSAYS OF
CARCINOGENIC OR SUSPECT CARCINOGENIC AGENTS AGAINST MARINE FISHES. WE
REPORT HERE THE DESIGN, RESULTS OF LONG TERM TESTS, AND THE FUTURE
USES OF THE SYSTEM FOR DETERMINING THE RISKS OF CARCINOGENIC AGENTS IN
THE AQUATIC ENVIRONMENT, AND AS A SYSTEM COMPLEMENTARY TO MAMMALIAN
ASSAY SYSTEMS* BUT WHICH PERMITS THE PHYLOGENETIC EXPANSION OF
CARCINOGEN ASSAY METHODOLOGY. A PILOT TEST OF THE DESCRIBED SYSTEM HAS
BEEN COMPLETED. THIS TEST UTILIZED FLOWING FILTERED, ESTUARINE WATER,
CONTROLLED WATER TEMPERATURE, CONTROLLED PHOTO PERIOD, CONTROLLED
NUTRITION OF TEST SPECIES, OXYGEN CONCENTRATION MONITORING, AND
VARIOUS LIFE CYCLE STAGES OF THE TEST FISH, THE SHEEPSHEAO MINNOW,
CYPRINODON VARIEGATUS, AND THE SUSPECT CARCINOGENIC HERBICIDE,
TRIFLURAL1N. CONTINUOUS EXPOSURES TO 1 TO 5 MG/L TRIFLURALIN WERE
CONDUCTED WITH ZYGOTE THRU EMBRYOGENESIS TO ADULT STAGES OF THE FISH.
COUCH* JOHN A., LEE A. COURTNEY, JAMES T. WINSTEAO, AND STEVEN S. FOSS.
1979. AMERICAN OYSTER CCRASSOSTREA VIRGINICA) AS AN INDICATOR OF CARCINOGENS
IN THE AQUATIC ENVIRONMENT. IN: ANIMALS AS MONITORS OF ENVIRONMENTAL
POLLUTANTS. NATIONAL ACADEMY OF SCIENCES, WASHINGTON, DC. PP. 65-84.
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COUCH, JOHN A., ANO CLYDE OftWE. IN PRESS. MOUSE VS MINNOW: THE FUTURE OF
FISH IN CARCINOGENICITY TESTING CA FRIENDLY DISCUSSION/DEBATE BY OR. JOHN
COUCH ANO OR, CLYDE Q4WE) - DEBATE: THE FISHY SIDE. IN: PROCEEDINGS OF THE
SYMPOSIUM ON THE USE OF SMALL FISH SPECIES IN CARCINOGENCITY TESTING,
BETHESOA, MARYLAND, DECEMBER 8-10, 1981. U.S. NATIONAL CANCER INSTITUTE.
CERL.GB 472).
THE TEXT IS PART OF A DEBATE ANO ADVOCATES THE USE OF SMALL FISH
SPECIES AS LABORATORY TEST ORGANISMS IN DETECTING AND DETERMINING
POTENCY ANO ACTIVITY OF SUSPECTED CARCINOGENS. THE USEFULNESS OF
FISH SPECIES IS COMPARED ANO CONTRASTED WITH THE USEFULNESS OF THE
MORE CONVENTIONAL ASSAY SPECIES* SUCH AS RODENTS.
COUCH, JOHN A., AND ELSAYEO ELNENAEY. IN PREP. COMPLEX CHROMATOPHOROMA IN A
MARINE TELEOST FISH, FUNDULUS GRANDIS: MORPHOLOGICAL AND BIOCHEMICAL
CHARACTERISTICS. CERL.GB 286).
THREE SPECIMENS OF FUNDULUS GRANOIS, THE GULF KILLIFISH, HAD PIGMENTED
TUMORS COMPRISED OF A CELL TYPE THAT HAD CHARACTERISTICS OF DIFFERENT
PIGMENT CELL PHENOTYPES. THE FISH WERE FROM A MARICULTURE ATTEMPT ON
THE GULF COAST OF ALABAMA USA, ANO WERE OLDER LARGER SPECIMENS OF OVER
6,000 F. GRANDIS EXAMINED. A DETAILED LILGHT MICROSCOPY, ELECTRON
MICROSCOPY, CHROMATOGRAPHIC, AND CELL CULTURE WAS CONDUCTED ON THE
TUMORS FROM THREE FISH. THE TUMOR CELL POSSESSED PTERINOSOMES AS THE
DOMINANT CYTOPLAMIC ORGANELLE, BUT ALSO HAD PREMELANOSOMES,
MELANOSOMES, ANO POSSIBLE REFLECTING PLATELETS. THE MAJOR PIGMENTS
ISOLATED AND IDENTIFIED WERE PTERIDINE PIGMENTS. NO CAROTENOID
PIGMENTS OR CAROTENOID VESTICLES WERE IDENTIFIED OR DETECTED. THE
TUMORS WERE INVASIVE, REPLACED AND ISOLATED NORMAL MUSCLE TISSUES, BUT
WERE NOT METASTATIC. ONE FISH WITH THE TUMORS BECAME MORIBUND WHILE
HELD IN AQUARIUM, ANO PRESENTED EVIDENCE THAT THE NEOPLASMS GREW AND
INCREASED IN NUMBERS. THE CAUSES OF THE NEOPLASM IN FUNDULUS ARE
UNKNOWN, BUT BOTH ENVIRONMENTAL ANO GENETIC FACTORS MAY PLAY ROLES IN
ITS GENESIS.
COUCH, JOHN A., FRANK G. LOWMAN, ANO FORD A. CROSS. 1980. 6IOMONITORING OF
COASTAL WATERS—AN OVERVIEW. IN: BIOLOGICAL MONITORING FOR ENVIRONMENTAL
EFFECTS. DOUGLAS L. WORF, EDITOR, D.C. HEATH AND COMPANY, LEXINGTON, MA.
PP. 93-95. CERL.GB X156).
ESTUARINE AND COASTAL WATERS OF THE UNITED STATES NOT ONLY SERVE AS
PRIME HABITAT FOR A SIGNIFICANT FRACTION OF COMMERCIAL AND
RECREATIONAL MARINE FISHERIES, BUT ALSO RECEIVE INDUSTRIAL ANO
MUNICIPAL WASTES FROM A RAPIDLY EXPANDING COASTAL ECONOMY. IN ORDER TO
ENSURE THAT LEVELS OF CONTAMINANTS IN COASTAL ECOSYSTEMS 00 NOT AFFECT
EITHER PUBLIC HEALTH OR FISHERIES RESOURCES ADVERSELY, BOTH STATE AND
FEDERAL AGENCIES ARE CONDUCTING A VARIETY OF BIOMONITORING PROGRAMS.
IN ADDITION, GENERIC RESEARCH PROJECTS ARE UNDERWAY AT SEVERAL
UNIVERSITY, STATE, ANO FEDERAL LABORATORIES TO DETERMINE WHICH
ENVIRONMENTAL AND PHYSIOLOGICAL FACTORS REGULATE THE BODY BURDEN OF
CONTAMINANTS IN MARINE ORGANISMS. THIS INFORMATION CAN THEN BE USED TO
HELP INTERPRET DATA OBTAINED IN BIOMONITORING PROGRAMS AND TO ALLOW
MORE PRECISE PREDICTIONS OF CONTAMINANT LEVELS IN BIOTA PRIOR TO
DISCHARGE.
PAGE
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COUCH, JOHN A., AND W. PETER SCHOOR. 1980. EFFECTS OF CARCINOGENS,
MUTAGENS, AND TERATOGeNS ON NON-HUMAN SPECIES (AQUATIC ANIHALS). IN: ANNUAL
REPORT ON NATIONAL CANCER INSTITUTE AND ENVIRONMENTAL PROTECTION AGENCY
PROJECTS, U.S. OEPT. OF HEALTH, EDUCATION, AND WELFARE, PUBLIC HEALTH
SERVICE, NATIONAL INSTITUTE OF HEALTH, BETHESDA, HO. 38P.
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COUCMt JOHN A., AMD W. PETER SCHOOR. 1982. EFFECTS OF CARCINOGENS*
MUTAGENS, AND TERATQGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS). IN:
PROCEEDINGS OF THE SECOND NCI/EPA/NIOSH COLLABORATIVE WORKSHOP: PROGRESS ON
JOINT ENVIRONMENTAL AND OCCUPATIONAL CANCER STUDIES, SEPTEMBER 9-11, 1981f
ROCKVILLE, MD. HERMAN F. KRAYBILL, INGEBORG C. BLACKWOOO, AND NANCY 8.
FREAS.EOITORS, U.S. GOVERNMENT PRINTING OFFICE, WASHINGTON, DC. PP. 688-726.
(ERL,GB 433).
AQUATIC SYSTEMS AND ORGANISMS ARE UNDER EXAMINATION IN ORDER TO
DEVELOP INDICATOR, SCREENING, AND MODELING CAPABILITIES FOR DETECTION
OF CARCINOGENS, MUTAGENS AND TERATOGENS. IN THE THIRD YEAR OF THE
PROGRAM, SEVERAL ADVANCES WERE MADE IN THE DEVELOPMENT OF ASSAYS,
UTILIZING SHEEPSHEAO MINNOWS CLIVER LESIONS VIA BENZIDINE AND
AFLATOXIN EXPOSURE), RAINBOW TROUT (LIVER TUMORS VIA BENZO
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COUCH, JOHN A., AND W.P. SCHOOR. 1981. EFFECTS OF CARCINOGENSt MUTAGENS*
AND TERATOGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS). IN: SECOND ANNUAL
REPORT ON NATIONAL CANCER INSTITUTE AND ENVIRONMENTAL PROTECTION AGENCY
PROJECTS. U.S. OEPT. OF HEALTH, EDUCATION, AND WELFARE, PUBLIC HEALTH
SERVICE* NATIONAL INSTITUTE OF HEALTH, 86THESDA, HO. PP. 57-103. (ERL,GB
X334).
THE SECOND ANNUAL REPORT OF THE GULF BREEZE LABORATORY AQUATIC
CARCINOGEN PROGRAM, CONTAINS INFORMATION ON ADVANCES AND PROGRESS IN
BOTH IN-HOUSE EFFORTS AND EXTRAMURAL COOPERATIVE AGREEMENT PROJECTS
THAT ARE SUPPLEMENTAL TO THE GOALS OF THE PROGRAM FOR FY 80. FLEXIBLE
FISH CARCINOGEN ASSAY SYSTEMS WITH OPTIONAL MOOES OF EXPOSURE OF BOTH
FRESHWATER ANO MARINE FISHES AND INVERTEBRATES HAVE BEEN DEVELOPED AND
TESTED FOR EFFICIENCY. THE SYSTEM AT GULF BREEZE IS VALID FOR EXPOSURE
OF FISHES THROUGH ENTIRE LIFE CYCLES TO ENVIRONMENTALLY REALISTIC
CONCENTRATIONS OF SUSPECT CARCINOGENS. RESULTS OF TRIFLURALIN
(HERBICIDE, SUSPECT CARCINOGEN) VERSUS SHEEPSHEAO MINNOW (TEST FISH)
INDICATE THAT THE SYSTEM IS VALID FOR CARCINOGEN TESTS. ANOTHER
EXPERIMENTAL ASSAY SYSTEM FOR RAINBOW TROUT HAS BEEN DEVELOPED AND
TESTED THROUGH THE COOPERATIVE AGREEMENT AT OREGON STATE UNIVERSITY,
WHILE FURTHER WORK HAS BEEN COMPLETED ON THE FRESHWATER CATFISH SYSTEM
AT THE UNIVERSITY OF SOUTHERN MISSISSIPPI. THE FIELD COLLECTION PHASE
OF THE EPIZOOTIOLOGY OF TUMORS AND CELLULAR DISEASES IN FISH ANO
SHELLFISH IN RELATION TO HUMAN POLLUTANT ACTIVITY HAS BEEN COMPLETED.
EARLY TRENDS INDICATE A POSITIVE RELATIONSHIP BETWEEN DISEASE
FREQUENCY ANO POLLUTANT ACTIVITY, BUT MUCH ANALYSIS OF DATA IS
INCOMPLETE AT THIS POINT. ANALYTICAL ADVANCES IN THE RESOLUTION ANO
IDENTIFICATION OF POLYCYCLIC AROMATIC HYDROCARBONS ANO THEIR
METABOLITES IN AQUATIC ORGANISMS HAVE BEEN MADE AT GULF BREEZE. WORK
IS PROGRESSING ON CHARACTERIZING THE RESPONSES OF LIVER ENZYME SYSTEMS
IN SEVERAL FISHES FROM BOTH FRESHWATER AND MARINE WATERS. NEW
COOPERATIVE AGREEMENTS ON STUDIES OF NATURAL POPULATIONS OF FISHES ANO
SALAMANDERS WITH RELATIVELY HIGH PREVALENCES OF TUMORS HAS BEGUN LATE
IN FY 80.
COUCH, JOHN A.t AND JAMES T. WINSTEAD. 1979. CONCURRENT NEOPLASTIC AND
PROTISTAN DISORDERS IN THE AMERICAN OYSTER (CRASSOSTREA VIRGINICA).
HALIOTIS. 8(2>:2*9-253. (ERL.GB 353).
ONE OF 373 OYSTERS EXAMINED AS PART OF A HISTOLOGICAL SURVEY OF
OYSTERS FROM APALACHICQLA BAY, FLORIDA, USA, HAD A CONCURRENT BLOOD
CELL PROLIFERATIVE DISORDER AND A PROTISTAN INFECTION. THE NEOPLASTIC
BLOOD CELLS (LEUKOCYTES) WERE FOUND THROUGHOUT THE VESICULAR
CONNECTIVE TISSUES AND BLOOD SPACES IN SECTIONS OF THE OYSTER. THESE
PROLIFERATING CELLS RESEMBLED THE NEQPLASTIC CELLS DESCRIBED BY COUCH
(1969) ANO FARLEY (1969) FROM OTHER SPECIMENS OF CRASSOSTREA VIRGINICA
FROM CHESAPEAKE BAY, MARYLAND, USA. MITOTIC FIGURES WERE ABUNDANT IN
FOCI OF THE NEOPLASTIC TISSUE. EPITHELIAL TISSUES OF THE GUT OF THIS
OYSTER WERE INFECTED BY SPORE AND SCHIZOGONIC STAGES OF OERMQCYSTIDIUM
MARIMUM, A PRESUMED PATHOGENIC PROTIST.OF OYSTERS. SPORES OF THIS
PROTIST AND OTHER PRQLIFERATIVE STAGES WERE OBSERVED IN CONNECTIVE
TISSUES ANO BLOOD SPACES. THERE WAS NO MORPHOLOGICAL EVIDENCE THAT THE
NEOPLASTIC BLOOD CELLS WERE RELATED TO THE PROTIST LIFE CYCLE STAGES.
PAGE
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COURTNEY, LEE A., ANO JOHN A. COUCH. IN PRESS. USEFULNESS OF CYPRINOOON
VARIEGATUS AND FUNOULUS GRANOIS IN CARCINOGENICITY TESTING: ADVANTAGES AND
SPECIAL PROBLEMS. IM: PROCEEDINGS OF SYMPOSIUM ON THE USE OF SMALL FISH
SPECIES IN CARCINOGEMCITY TESTING, BETHESDA, MARYLAND, DECEMBER 8-10, 1981.
U.S. NATIONAL CANCER INSTITUTE. (ERL,G8 442).
CYPRINOOON VARIEGATUS AND FUNOULUS GRANOIS, TWO SPECIES OF
CYPRXNQOQNTIO FISHES EXTENSIVELY STUDIED AND USED IN TOXICOLOGICAL AND
BIOLOGICAL INVESTIGATIONS, ARE COMPARED AS LABORATORY TEST ANIMALS.
THEIR ECOLOGY AND GENERAL BIOLOGY, ANO SUITABILITY FOR VARIOUS TYPES
OF EXPERIMENTATION ARE EXAMINED. A LABORATORY SYSTEM FOR EXPOSING
CRITICAL LIFE STAGES (E.G., EMBRYOS, FRY, JUVENILES) OF THESE SPECIES
TO SUSPECT CARCINOGENS IS DESCRIBED. A DISCUSSION OF THE USE,
FINDINGS* AND POTENTIAL OF THESE SPECIES IN ONCOLOGICAL STUDIES AND
CARCINOGEN ASSAYS IS PRESENTED* PARTICULARLY IN REGARD TO RESPONSES TO
THREE KNOWN OR SUSPECT CARCINOGENIC CHEMICALS (E.G., TRIFLURALIN,
BENZIOINE, ANO AFLATOXIN). FINALLY, ADVANTAGES ANO DISADVANTAGES OR
SPECIAL PROBLEMS IN USING THE SPECIES AS CARCINOGEN TEST ANIMALS ARE
REVIEWED.
ELNENAEY* ELSAYED A.* ANO W. PETER SCHOOR. 1981. SEPARATION OF THE ISOMERIC
PHENOLS OF BENZOCA)PYRENE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. ANAL.
BIOCHEM. 111C2):393~400. CERL,GB 046*).
A TfCHNIQUE HAS BEEN DEVELOPED TO SEPARATE A MIXTURE OF THE TWELVE
ISOMERIC PHENOLS OF B£NZO
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ELNENAEY, ELSAYEO, AND W.P. SCHQOR. 1981. SIMPLE HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY METHOD FOR SEPARATION OF BENZO(A)PYRENE METABOLITES
(ABSTRACT)* CLIN. CHEM. 27€6):i099. (ERLtGS X344).
TO CONTINUE OUR STUDY TO DEVELOP A RELIABLE METHOD FOR QUALITATIVE AND
QUANTITATIVE SEPARATION OF BENZO(A)PYRENE CBAP) METABOLITES, WE REPORT
A SIMPLEt RAPID HPLC USING PERKIN ELMER DOS SIL-X COLUMN AND A MIXTURE
OF METHANOL AND HATER AS ISOCRATIC ELUANT. AS WE PREVIOUSLY REPORTED
(J. ANAL. 8IOCHEH., IN PRESS) BOTH THE FLOW RATE AND SOLVENT
COMPOSITION HAVE DIFFERENT EFFECTS ON SEPARATION PATTERN. THE USE OF
BOTH, FLUORESCENCE AND U.V. DETECTION SIMULTANEOUSLY WAS FOUND TO BE
OF GREAT IMPORTANCE CONCERNING THE FACT THAT SOME METABOLITES E.G.
OZONES MAY HAVE RELATIVELY GOOD ABSORPTION WHILE THE OTHER METABOLITES
(PHENOLS AND OIOLS) HAVE EXCELLENT FLUORESCENCE ACTIVITY AND
RELATIVELY POOR ABSORPTION. THE ABSORPTION WAS MONITORED AT 254 N« AND
FLUORESCENCE (EX. 263 NM AND EM. 370 FILTER). THE USE OF AN ISOCRATIC
SOLVENT (65* METHANOL IN WATER) ELIMINATES USE OF ANY DRIFT OF THE
BASELINE SPECIALLY FOR THE UV SIDE IF COMPARED TO A GRADIENT SYSTEM.
THE FLOW RATES RANGING FROM 0.3-0.6 ML/MIN. WERE TESTED AND THE 0.4
ML/MIN. RESULTED IN A GOOD SEPARATION. THE FOLLOWING BAP DERIVATIVES
WERE SEPARATED IN ONE CHROMATOGRAM (T)9,10-,
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GREGORY, PAULA £., PATRICIA N. HOWARD-PEEBLES, RUDOLPH 0. ELLENOER, AND BILLY
J. MARTIN. 1980. C-BANOING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE
FISH CELL LINES. COPEIA. C3):545-547. CERL.GB X199).
DIFFERENTIAL GIEMSA STAINING OF TH CONSTITUTIVE HETEROCHROMATIC IN
HUMAN CHROMOSOMES HAS BECOME A USEFUL ANO ACCEPTED TECHNIQUE CARRIGHI
AND HSU* 1971). SUCH A STAINING TECHNIQUE COULO BE OF GREAT VALUE WHEN
APPLIED TO THE CHROMOSOMES OF OTHER ORGANISMS, ESPECIALLY THOSE WITH
NUMEROUS MORPHOLOGICAL IDENTICAL CHROMOSOMES. MANY FISH KARYOTYPES
POSSESS A LARGE GROUP OF MORPHOLOGICALLY SIMILAR CHROMOSOMES. A
TECHNIQUE HAS BEEN DEVELOPED THROUGH MODIFICATION OF A C-BANDING
PROCEDURE THAT PRODUCES DISTINCT BANDS ANO THUS ALLOWS IDENTIFICATION
OF THE HETEROCHROMATIC REGIONS OF INDIVIDUAL PAIRS OF FISH
CHROMOSOMES. THIS TECHNIQUE HAS BEEN APPLIED TO THREE ESTABLISHED
MARINE FISH CELL LINES. C-BANDING HAS BEEN REPORTED ON SEVERAL TROUT
SPECIES (ABE ANO MURAMOTO, 1974; ZENZES AND VQICULESCU, 1975!
THORGAARD, 1976); HOWEVER, NONE OF THESE STUDIES EMPLOYED ESTABLISHED
CELL LINES.
GRIZZLE, J.M., T.E. SCHWEDLER, AND A.L. SCOTT. 1981. PAPILLOMAS OF BLACK
bULLHEADS, ICTALURUS MELAS CRAFINESQUE), LIVING IN A CHLORINATED SEWAGE POND,
J. FISH DISEASES. 4COS345-351. (ERL,GB X250).
PAPILLOMAS ON BROWN BULLHEADS, ICTALURUS NEBULOSUS
-------
HARSH3ARGER, JOHN C.v ELLIOT R. JACOBSON, CHARLIE E. SMITH, AND JOHN A.
COUCH. 1980. HEMATOPOIETIC NEOPLASMS IN INVERTEBRATES AND COLO-BLOODED
VERTEBRATES. IN: ADVANCES IN COMPARATIVE LEUKEMIA RESEARCH, 1979, OAVIO S.
YOHN, BORIS A. LAPIN, AND JAMES R. 3LAKESLEE, EDITORS,
ELSEVIER/NORTH-HQLLAND, NEW YORK, NY. PP. 223-225. CERL.GB X158).
MANY EXCELLENT EXAMPLES OF HEMATOPOIETIC NEOPLASMS OCCUR IN
INVERTEBRATE AND COLD-BLOOOED VERTEBRATE ANIMALS. SINCE THESE GROUPS
COMPRISE OVER 99% OF THE ANIMAL KINGDOM AND CONTAIN AN ENORMOUS
DIVERSITY COMPARED TO WARM-BLOODED ANIMALS, THEY COULD UNOOU8TLY
PROVIDE MODELS OPTIMALLY SUITED FOR TESTING SPECIFIC HYPOTHESES. I
RECOMMEND CANCER RESEARCHERS CONSIDER THIS POTENTIAL IN PLANNING
FUTURE EXPERIMENTS.
HENDRICKSt J. D.« T. R. MEYERS, AND D. W. SHELTON. IN PRESS. HISTOLOGIC
PROGRESSION OF HEPATIC NEOPLASMS IN RAINBOW TROUT CSALMO GAIRDNERI). IN:
PROCEEDINGS OF SYMPOSIUM ON THE USE OF SMALL FISH SPECIES IN CARCINOGENCITY
TESTING. U.S. NATIONAL CANCER INSTITUTE. (ERL,G3 X353).
HENORICKS, J.D., R.Q. SINNHUBER, H.M. LOVELANO, N.E. PAWLOWSKI, AND J.E.
NIXON. 1980. HEPATOCARCINOGENICITY OF GLANDLESS COTTONSEEDS AND REFINED
COTTONSEED OIL TO RAINBOW TROUT (SALMO GAIRDNERI). SCIENCE.
208(4440:309-310.
-------
HENORICKS, J.O., J.H. WALES, R.O. SINNHU3ER, J.E. NIXON, P.M. LQVELANO, AND
R.A. SCANLAN. 1980. RAINBOW TROUT CSALMO GAIRDNERI> EMBRYOS: A SENSITIVE
ANIMAL MODEL FOR EXPERIMENTAL CARCINOGENESIS. FED. PROC. 39C14)13222-3229.
(£RL,GB X359).
DIETARY EXPOSURES HAVE DEMONSTRATED RAINBOW TROUT TO BE THE MOST
SENSITIVE EXPERIMENTAL ANIMAL TO THE H=PATOCARCINOGENICITY OF
AFLATOXIN BU> CAFB). MORE RECENTLY THE DEVELOPMENT OF AN ALTERNATE
EXPOSURE METHOD HAS SHOWN TROUT TO BE EVEN MORE SENSITIVE TO AFBC1).
THIS METHOD INVOLVES THE SINGLE EXPOSURE OF FERTILE RAINBOW TROUT EGGS
(EMBRYOS) TO A 0.5 PPM AQUEOUS SOLUTION OF AFBC1) FOR 30 MINUTES*
RINSING THE EGGS IN WATER, AND ALLOWING HATCHING, SWIM-UP AND THE
ONSET OF FEEDING TO PROCEED AS USUAL. RESULTING FISH ARE FED A CONTROL
DIET FOR 1 YEAR AT WHICH TIME APPROXIMATELY TWO-THIRDS OF THE
POPULATION WILL HAVE DEVELOPED HEPATOCELLULAR CARCINOMA OF THE LIVER.
VARIABLES, SUCH AS EMBRYO AGE OF GREATEST SENSITIVITY, CARCINOGEN
CONCENTRATION, AND LENGTH OF EXPOSURE, HAVE BEEN DEFINED FOR AF8CD
EXPOSURES. SENSITIVITY AFBC1) INCREASED WITH INCREASING AGE OF THE
EMBRYO, THE MODEL SYSTEM SHOWED A DOSE-RESPONSE TO INCREASING
CARCINOGEN CONCENTRATION, AND ONE-HALF HOUR EXPOSURES APPEARED TO BE
OPTIMUM FOR AF8U). EXPERIMENTS WITH OTHER CARCINOGENS HAVE SHOWN THE
EMBRYO MODEL SYSTEM TO BE SENSITIVE TO THE HEPATOCARCINOGENICITY OF
AF8<1) METABOLITES (AFLATOXICOL, AFLATOXIN MCI), AND AFLATOXIN G(l»,
STERIGMATOCYSTIN, VERSICOLORIN A, OIMETHYLNITROSAMINE, AND N-METHYL-N"
-NITRO-N-NITROSOGUANIDINE. THE LATTER COMPOUND ALSO INITIATED
NEPHROBLASTOMAS OF THE KIDNEYS. THE RESULTS DEMONSTRATE THE POTENTIAL
FOR THE TROUT EMBRYO TO BE DEVELOPED INTO A CONVENIENT, ECONOMICAL,
AND SENSITIVE WHOLE ANIMAL MODEL SYSTEM FOR EXPERIMENTAL
CARCINOGENESIS.
HENORICKS, JERRY 0. 1982. CHEMICAL CARCINOGENESIS IN FISH. IN: AQUATIC
TOXICOLOGY, VOL. 1. LAVERN J. WEBER, EDITOR, RAVEN PRESS, NEW YORK. PP.
149-211. CERL.GB X251>.
MOST OF THE RESEARCH ON EXPERIMENTAL CARCINOGENESIS IN SALMONIDS HAS
BEEN CONDUCTED BY TWO RESEARCH GROUPS. EARLY STUDIES INTO THE ETIOLOGY
OF THE TROUT LIVER CANCER EPIZOOTIC WERE PERFORMED AT THE U.S, FISH
AND WILDLIFE SERVICE'S WESTERN FISH NUTRITION LABORATORY AT COOK,
WASHINGTON, UNDER THE DIRECTION OF DRS. JOHN E. HALVER AND LAURENCE M.
ASHLEY. A CONTINUING STUDY OF THE METABOLISM AND CARCINOGENICITY OF
AFB AMD ITS METABOLITES IN RAINBOW TROUT, AS WELL AS THE DEVELOPMENT
OF THE RAINBOW TROUT AS A DOCUMENTED RESEARCH ANIMAL FOR CARCINOGENIC
AND TOXICOLQGIC RESEARCH, HAS OCCURRED FOR THE PAST 16 YEARS AT THE
FOOD TOXICOLOGY AND NUTRITION LABORATORY CFTNL> IN THE DEPARTMENT OF
FOOD SCIENCE AND TECHNOLOGY AT OREGON STATE UNIVERSITY UNDER THE
DIRECTION OF PROFESSOR RUSSELL 0. SINNHUBER.
HENDRICKS, JERRY 0., THEODORE R. MEYERS, JOHN L. CASTEEL, JOE E. NIXON,
PATRICIA M. LOVELANO, AND GEORGE S. BAILEY. 1982. RAINBOW TROUT EMBRYOS?
ADVANTAGES AND LIMITATIONS FOR CARCINOGENESIS RESEARCH (ABSTRACT), AMNU.
MEET, AM, ASSOC. CANCER RES. PROC. 23:58. CERL.GB X279).
PAGE 14
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HENORICKS, JERRY 0.. THEODORE R. MEYERS, DENNIS W. SHELTON, AND RUSSELL 0,
SINNHUBER. 1982. LIVER NEOPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION
OXIOASE ENZYMES THE RAINBOW TROUT FOLLOWING DIETARY EXPOSURE TO
6ENZQCA)PYREN£ (ABSTRACT). ANNU. MEET. AM. ASSOC. CANCER RES. PRQC. 23:58.
(ERLtGB X258).
THE INFLUENCE OF BENZO
AND CYTOCHROME P-450 WERE MEASURED DURING THIS TIME. A FEEDING TRIAL
USING A MTD OF 1000 PPM WAS INITIATED IN DUPLICATE GROUPS OF 100
FINGERLING RAINBOW TROUT. TROUT WERE SAMPLED AT 6, 12 AND 18 MONTHS
FOR NECROPSY AND SUBSEQUENT GROSS AND HISTOLOGICAL EXAMINATION OF
INTERNAL ORGANS. MEAN MFO ENZYME LEVELS OF EROO, ECOD, AND AHH WERE
SIGNIFICANTLY
-------
KOENIG, CHRISTOPHER C.* DANIEL C. ABEL, COURTNEY W. KLINGENSMITH, AND MICHAEL
B. MAOOOCK. 1982, USEFULNESS OF THE SELF-FERTILIZING CYPRINOOONTID FISH,
RIVULUS MARMQRATUS AS AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING
CARCINOGENESIS, TERATQGENESIS AND MUTAGSNESIS. EPA-600/3-82-075, U.S.
ENVIRONMENTAL PROTECTION AGENCY, ENVIRONMENTAL RESEARCH LABORATORY, GULF
BREEZE, FL. 129P.
RIVULUS MARMORATUS IS A NATURALLY SELF-FERTILIZING CYPRINOOONTIO FISH
INHABITING MANGROVE MARSHES THROUGHOUT THE CARIBBEAN. AS A RESULT OF
INTERNAL SELF-FERTILIZATION THIS OVIPAROUS SPECIES IS COMPOSED OF A
NUMBER OF ISOGENIC, HOMOZYGOUS LINES (CLONES), SEVERAL OF WHICH HAVE
BEEN IDENTIFIED BY HISTOCOMPATIBILITY EXPERIMENTS AND MAINTAINED IN
LABORATORY CULTURE FOR OVER 30 YEARS. SIMPLIFIED CULTURE AND HANDLING
METHODS ARE GIVEN AND DATA ARE PRESENTED ON THE REPRODUCTION, GROWTH
AND DEVELOPMENT OF RIVULUS UNDER LABORATORY CULTURE AS A PRELUDE TO
THE EVALUATION OF ITS POTENTIAL AS A BIOASSAY ANIMAL. SEVERAL TYPES OF
BIOASSAYS WERE RUN AND EVALUATED USING RIVULUS: BEHAVIORAL,
CARCINQG£NICXTY,TERATOGENICITY, TOXICITY, AND MUTAGENICITY. ADVANTAGES
AND DISADVANTAGES OF USING RIVULUS FOR SUCH BIOASSAYS ARE DISCUSSED.
BEHAVIORALLY, RIVULUS IS CAPABLE OF DETECTING AND AVOIDING WATER
CONTAMINATED WITH H2S. THEY RESPOND (EC50 * 123.6 PPB H2S) BY LEAPING
FROM THE WATER AND REMAINING EMERGENT FOR VARIOUS PERIODS OF TIME
WHILE RESPIRING CUTANEOUSLY. HEPATOCELLULAR CARCINOMA AMONG OTHER
PATHOLOGIC CHANGES WERE OBSERVED IN LIVERS OF RIVULUS A YEAR AFTER
EXPOSURE OF ADULTS AND LARVAE TO DIETHYLNITROSAMINE C45, 30, AND 15
PPM IN WATER) FOR 5 WEEKS AND 12 WEEKS, RESPECTIVELY. NO PATHOLOGIC
CHANGES WERE FOUND IN EMBRYOS EXPOSED SIMILARLY. HIGH RATES OF VARIOUS
SKELETAL MALFORMATIONS RESULTED IN OFFSPRING OF ADULTS EXPOSED TO
DIBUTYL PHTHALATE CDBP) AND 2*3,4,6-TETRACHLOROPHENOL CTECP) AT
CONCENTRATIONS OF 20, 10 AND 51 CD3P - 0.740, 0.370, 0.185 MG/LJ TECP
• 0.220, 0.110, 0.055 MG/L) OF THE LARVAL 96-HOUR LC50. NO
DOSE-RESPONSE RELATIONSHIPS OF SKELETAL MALFORMATIONS WERE FOUND FOR
SIMILAR EXPOSURES TO PENTACHLOROPHENQL, 2,3,5-TRICHLOROPHENOL OR
BROMOFORM, HOWEVER, CHRONIC EXPOSURE OF DEVELOPING HATCHLINGS TO TECP
RESULTED IN FIN AND GILL EROSION AND CHRONIC EXPOSURE TO BROMOFORM
PRODUCED DORSAL FIN ABNORMALITIES. AS PART OF A MUTAGENESIS BIOASSAY
14 ENZYME SYSTEMS REPRESENTING 28 LOCI WERE SCREENED FOR THE THREE
LABORATORY CLONES AND ONE WILD-CAUGHT CLONE BUT NO 6LECTRQPHORETIC
DIFFERENCES WERE FOUND. ATTEMPTS TO CULTURE RIVULUS CELLS FAILED.
ALSO, THE KARVOTYPE OF RIVULUS IS NOT SUITABLE FOR SHORT-TERM
CYCTOGENETIC ASSAYS SUCH AS THE SISTER CHROMATID EXCHANGE CSCE) ASSAY.
ALTERNATIVELY, HOWEVER, THE TQADFISH
-------
LATOUCHE, Y. DAVID, CASEY W. 8ENNETT, AND MICHAEL C. MIX. 1981.
DETERMINATION OF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EXCHANGE
RESIN NEUTRON ACTIVATION. BULL. ENVIRON. CONTAM. TOXICOL. 26(2):224-227.
CeRLtGB X351).
STUDIES DESIGNED TO MEASURE CONTAMINANTS IN THE MARINE AND ESTUARINE
ENVIRONMENT HAVE GENERALLY NEGLECTED VANADIUM. ENVIRONMENTAL VANADIUM
HAS BEEN CONSIDERED AS A TRACE CONSTITUENT OF PETROLEUM WHICH HAS
LEVELS RANGING, FOR EXAMPLE* FROM 0.6 TO 1400 PPM IN DIFFERENT
VENEZUELAN CRUDE OILS CNAS 1974). VARIABLE VANADIUM CONCENTRATIONS
CHARACTERIZE DIFFERENT PETROLEUMS; SUCH "FINGERPRINTS" IN OIL SPILL
SAMPLES HAVE BEEN USEFUL IN ESTABLISHING RESPONSIBILITY FOR POLLUTION
INCIDENTS CGUINN ET AL. 1971).
LATOUCHE, Y. DAVID, AND MICHAEL C. MIX, 1981. SEASONAL VARIATION IN SOFT
TISSUE WEIGHTS AND TRACE METAL 8UROENS IN THE BAY MUSSEL, MYTILUS EDULIS.
BULL. ENVIRON. CONTAM. TOXICOL. 27(6):821-828. (ERL,GB X310).
THE PURPOSE OF THE PRESENT STUDY WERE TO MEASURE THE LEVELS OF 6 TRACE
METALS IN M. EDULIS SOFT TISSUES DURING A 9-MONTH PERIOD, ESTABLISH
PATTERNS OF SEASONAL VARIATION AND DETERMINE THE NORMAL RANGES OF
METAL BURDENS IN MUSSELS. THE METALS WERE VANADIUM (V), MANGANESE
» NICKEL CNDt COPPER CCU), ZINC (ZN), AND CADMIUM CCD); NI, CU,
ZN, AND CO ARE EPA PRIORITY POLLUTANTS.
LATOUCHE, Y. DAVID, AND MICHAEL C. MIX. 1982. EFFECTS OF DEPURATION, SIZE
AND SEX ON TRACE METAL LEVELS IN BAY MUSSELS. MAR. POLLUT. BULL.
13(1)827-29. (£RL,GB X412).
THE EFFECTS OF DEPURATION, SIZE AND SEX ON THE CONCENTRATIONS OF MN,
NI, CU, ZN AND CO WERE DETERMINED FOR GONAOAL AND SOMATIC TISSUES OF
MYTILUS EDULIS FROM YAQUINA BAY, OREGON. AFTER DEPURATION, MN LEVELS
DECREASED SIGNIFICANTLY IN BOTH TISSUES WHILE CU AND NI CONCENTRATIONS
INCREASED IN SOMATIC TISSUES. THE LATTER FINDING MAY HAVE BEEN
ASSOCIATED WITH A STRESS RESPONSE MANIFESTED BY THE APPEARANCE OF NEW
BYSSAL THREADS AND ATTACHMENT TO THE VESSEL. LARGER, AND PRESUMABLY
OLDER, MUSSELS CONTAINED HIGHER CONCENTRATIONS OF NI, CU AND CD IN
THEIR SOMATIC TISSUES WHILE SMALLER ANIMALS HAD GREATER SOMATIC LEVELS
OF MN. THERE WERE NO SIGNIFICANT DIFFERENCES IN GONAO CONCENTRATIONS
BETWEEN THE TWO SIZE GROUPS. MANGANESE AND ZN LEVELS WERE GREATER IN
THE GONADS OF FEMALE MUSSELS AND CD WAS MORE CONCENTRATED IN THE
SOMATIC TISSUES OF FEMALES.
LATOUCHE, Y. OAVIO, ANO MICHAEL C. MIX. 1982. SEASONAL VARIATIONS OF
ARSENIC ANO OTHER TRACE ELEMENTS IN BAY MUSSELS (MYTILUS EOULIS). BULL.
ENVIRON. CONTAM. TOXICOL. 29(6):665-670. (ERL,GB X418).
THE PURPOSES OF THIS STUDY WERE TO INVESTIGATE SEASONAL VARIATIONS OF
ARSENIC IN A POPULATION OF BAY MUSSELS (MYTILUS EDULIS) ANO TO COMPARE
RESULTS WITH VARIATIONS OF SEVERAL OTHER TRACE ELEMENTS MORE COMMONLY
MEASURED IN ENVIRONMENTAL STUDIES. SEASONAL VARIATIONS OF MANGANESE,
NICKEL, COPPER, ZlNC, AND CADMIUM WERE INVESTIGATED PREVIOUSLY
(LATOUCHE & MIX 1981), BUT ARSENIC AND ITS RELATIONSHIP WITH THOSE
METALS IS NOW REPORTED FOR THE FIRST TIME.
PAGE 17
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LATOUCHE, YVES 0. 1981. TRACE METAL LEVELS IN A POPULATION OF MYTILUS
EDULIS FROM YAQUINA BAY, OREGON. PH.D. THESIS. OREGON STATE UNIVERSITY,
CORVALLIS, OR. 1359. (ERL,G8 X408).
THE PRESENT STUDY IS CONCERNED WITH THE M. EDULIS REPRODUCTIVE CYCLE
AND ITS EFFECT ON SOFT TISSUE CONTENT OF SIX TRANSITION METALS THAT
ARE IMPORTANT IN BIOLOGICAL FUNCTION AND/OR AS POLLUTANTS IN ESTUARINE
ENVIRONMENTS. A NATURAL POPULATION WITH A SUITABLY LARGE NUMBER OF
INDIVIDUALS FROM A LOCATION RELATIVELY FREE FROM METAL POLLUTANTS OF
ANTHROPOGENIC ORIGIN, WAS CHOSEN FOR THE INVESTIGATION. THE SEXUAL
STATE OF THE MUSSELS WAS DETERMINED BY MEASURING THE WEIGHT OF GONAOAL
MATERIAL RELATIVE TO SOMATIC TISSUE WEIGHT. METAL BURDENS IN GONADAL
AND SOMATIC TISSUES WERE DETERMINED AT REGULAR INTERVALS FOR ANIMALS
WITHIN A SINGLE-SIZE RANGE. THIS PROCEDURE HAD THE SIGNIFICANT
ADVANTAGE OF EXCLUDING ANY EXTRINSIC FACTORS THAT MIGHT HAVE IMPINGED
ON A POPULATION OF ANIMALS MAINTAINED IN A LABORATORY FOR A RELATIVELY
LONG PERIOD (8 MONTHS). IT WAS ALSO ESSENTIAL THAT NO UNNATURAL
STIMULI THAT MIGHT HAVE INDUCED SPAWNING BE PRESENT, AND THAT NO
ORDINARY ENVIRONMENTAL CUES BE EXCLUDED. SEVERAL ENVIRONMENTAL
MONITORING PROGRAMS WILL BE DISCUSSED WITH RESPECT TO THE METHODS THAT
THEY EMPLOY, AND THE TYPE AND QUALITY OF TjiE DATA THAT THEY PROVIDE.
RESULTS OF THIS STUDY WILL INDICATE HOW SUCH DATA MAY 3E IMPROVED, AND
ERRORS REDUCED BY MODIFICATION OF EXISTING PROCEDURES. THIS WILL LEAD
TO DEFINING PROCEDURES THAT SHOULD BE USED IN MONITORING PROGRAMS. THE
METALS OF CHOICE WERE VANADIUM, MANGANESE, NICKEL, COPPER, ZINC, AND
CADMIUM. NICKEL, COPPER, ZINC, AND CADMIUM ARE EPA PRIORITY
POLLUTANTS.
LONG, R.L., AND 6.J. MARTIN. 1980. MORPHOLOGY OF PERIPHERAL BLOOD CELLS OF
CYPRINOOON VARIEGATUS (ABSTRACT). J. MISS. ACAD. SCI. 26(SUPPL):123.
-------
MARTIN, B.J. 1980. EFFECTS OF PETROLEUM COMPOUNDS ON ESTUARINE FISHES.
EPA-600/3-80-019, U.S. ENVIRONMENTAL PROTECTION AGENCY, ENVIRONMENTAL
RESEARCH LABORATORY, GULF BREEZE, FL. 31P.
EFFECTS OF THE CARCINOGENIC POLYCYCLIC AROMATIC HYDROCARBONS (PAH),
BENZO(A)PYREN£ (BAP), AND METHYLCHOLANTHRENE (MCA) WERE INVESTIGATED
WITH SHEEPSHEAD MINNOWS CCYPPINODON VARIEGATUS) AND CHANNEL CATFISH
(ICTALURUS PUNCTATUS). A CLOSED-CIRCULATING SYSTEM WAS DESIGNED TO
MAINTAIN UP TO 100 SHEEPSHE&O MINNOWS IN ARTIFICIAL SEAWATER FOR
LONG-TERM EXPOSURES. FISH WERE MAINTAINED IN THIS SYSTEM FOR UP TO 31
WEEKS WITH WEEKLY CONTAMINATIONS OP PAH. DUE TO THEIR CHEMICAL
PROPERTIES SIGNIFICANT LEVELS OF BAP AND MCA REMAINED IN THE WATER
COLUMN FOR ONLY CA. 24 HOURS EACH WEEK AND NO TUMORS WERE OBSERVED IN
THE EXPOSED FISH DURING THE PERIOD OF THE STUDY. THE INCIDENCE AND
TYPES OF LESIONS IN CONTROL AND EXPOSED FISH WERE BASICALLY SIMILAR
EXCEPT IN CATFISH THAT WERE FED PAH CONTAMINATED FOOD. HIGH LEVELS OF
CONTAMINATION (1MG/GM FOOD) APPEARED TO BE TOXIC AND LOWER LEVELS OF
CONTAMINATION (0.1 MG/GM FOOD) PRODUCED SUFFICIENT STRESS TO MAKE THE
CATFISH SUSCEPTIBLE TO FATAL PARASITE INFESTATIONS. BOTH SPECIES
ACCUMULATED RAOIOACTIVELY LABELLED PAH AT CONCENTRATIONS MUCH HIGHER
THAN THEIR NORMAL CONCENTRATIONS IN THE WATER. THESE RESLTS
DEMONSTRATE THAT SHEEPSHEAO MINNOWS FUNCTION WELL AS EXPERIMENTAL
ORGANISMS IN ARTIFICIAL SEAWATER IN A CLOSED SYSTEM MAINTAINED AT A
NONCOASTAL FACILITY. THUS* THEY PROVIDE AN EXCELLENT MODEL SYSTEM FOR
THE STUDY OF LONG-TERM EFFECTS OF CHRONIC EXPOSURE TO POLLUTING
AGENTS.
MARTIN, 3.J. 1982. DEVELOPMENT OF A CARCINOGEN ASSAY SYSTEM UTILIZING
ESTUARINi FISHES. EPA-600/3-82-091, U.S. ENVIRONMENTAL PROTECTION AGENCY,
ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, FL. SOP.
THE OBJECTIVE OF THIS PROJECT WAS THE DEVELOPMENT OF SYSTEMS TO ASSAY
THE EFFECTS OF CHEMICAL CARCINOGENS ON MARINE TELEOSTS. IT WAS
DETERMINED THAT LC-50 FOR BENZINDINE WITH RESPECT TO CYPRINODON
VARIEGATUS WAS CA. 64 PPM. WEEKLY CONTAMINATIONS OF 1 PPM BENZIOINE
CAUSED SOME INDIVIDUALS TO DEVELOP PROLIFERATIVE LIVER LESIONS.
EXPOSURE OF C. VARIEGATUS EARLY EMBRYOS PRODUCED THE FOLLOWING
ANOMALIES AT CONCENTRATIONS OF 50 PPM AND ABOVE: TUBED HEART SYNDROME
WITH DISTENDED PERICARDIA, POOR CIRCULATION, SPARSE DISTRIBUTION OF
MELANOPHORES* INABILITY TO HATCH, ABNORMAL HEAD MORPHOLOGY, SCQLIOSIS,
AND FAINT RBC PIGMENTATION. CHRONIC EXPOSURE OF A CELL LINE FROM
ARCHOSARGUS PROBATOCEPHALUS TO BENZIOINE AND 8ENZCKA)PYRENE PRODUCED
MUTAGENIC EFFECTS. TWO NOVEL TECHNIQUES WERE DEVELOPED TO STUDY THE
EFFECTS OF CARCINOGENS ON C. VARIEGATUS AT THE CELLULAR LEVEL — AN
ASEPTIC EMBRYO TECHNIQUE AND AN EMBRYO-PRIMARY CELL CULTURE TECHNIQUE.
STANDARD IMMUMOLOGICAL TECHNIQUES WERE MINIATURIZED TO STUDY THE
IMMUNE SYSTEM OF C. VARIEGATUS. SERUM ELECTROPHORESIS DISCLOSED THAT
TH6 SERUM PROTEINS OF BENEZIDINE-EXPQSED FISH DIFFERED FROM UNEXPOS6D
CONTROLS.
PAGE 19
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MARTIN, 3.J., R.O. ELLENDER, S.A. HILLEBERT, AND M.M. GUESS. IN PRESS.
PRIMARY CELL CULTURES FROM THE TELEOST, CYPRINOOON VARIEGATUS: CULTURE
ESTABLISMENT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES. IN:
PROCEEDINGS OF TH£ SYMPOSIUM ON THE USE OF SMALL FISH SPECIES IN
CARCINOGENICITY TESTING, 8ETHESOA, MARYLAND, DECEMBER 8-10, 1981. U.S.
NATIONAL CANCER INSTITUTE. (ERL.GB X260).
METHODS WER£ DEVELOPED TO MAINTAIN C. VARIEGATUS FRY ASEPTICALLY FOR
EXTENDED PERIODS. PRELIMINARY STUDIES INDICATED THAT UNDER OPTIMUM
CONDITIONS STERILE EMBRYOS DEVELOP NORMALLY FOR A SUFFICIENT TIME TO
FUNCTION AS CARCINOGEN/TERATQGEN ASSAY SYSTEMS. AN EMBRYO-PRIMARY CELL
CULTURE TECHNIQUE WAS DEVELOPED THAT INCORPORATES, IN A SINGLE SYSTEM,
CERTAIN CHARACTERISTICS OF 80TH INTACT EMBRYOS AND PRIMARY CELL
CULTURES. ALLOWING SIMULTANEOUS OBSERVATION OF THE EFFECTS OF
CARCINOGENS ON THE WHOLE ORGANISM AND PRIMARY CELL MONOLAYERS. THE
EFFECTIVE USE OF THESE SYSTEMS PROVIDES THE OPPORTUNITY TO STUDY THE
EFFECTS OF CARCINOGENS ON TELEOSTS AT THE CELLULAR AND ORGANISMIC
LEVEL.
MARTIN, B.J., AND W.W. GREENWICH. 1980. EXPOSURE OF TWO TELEOST SPECIES TO
POLYCYCLIC AROMATIC HYDROCARBONS (ABSTRACT). J. MISS. ACAO. SCI.
XXV(SUPPL.):120. (£RL,GB X19O.
SHEEPSHEAO MINNOWS, CYPRINODON VARIEGATUS, AND CHANNEL CATFISH,
ICTALURUS PUNCTATUS, WERE MAINTAINED IN WATER CONTAMINATED WEEKLY WITH
POLYCYCLIC AROMATIC HYDROCARBONS (PAH). IN OTHER EXPERIMENTS, FISH
WERE MAINTAINED ON PAH CONTAMINATED FOOD REGIMES. BECAUSE OF THE
SOLUBILITY PROPERTIES OF PAH, SIGNIFICANT LEVELS DID NOT REMAIN IN THE
WATER COLUMN. HIGH LEVELS OF FOOD CONTAMINATION APPEARED TO STRESS
CATFISH SUFFICIENTLY TO INCREASE THEIR SUSCEPTIBILITY TO PARASITIC
INFESTATION. EXPERIMENTS IN WHICH RADIOACTIVELY LABELLED PAH WAS
PLACED IN THE WATER COLUMN INDICATED A RATHER VARIABLE
BIOACCUMULATION. HIGHEST LEVELS OF LABEL WERE OBSERVED IN GILL AND
LIVER TISSUE, AN INTERMEDIATE LEVEL IN GI TRACT, AND A LOWER LEVEL IN
SKELETAL MUSCLE. THE VARIABILITY OF THESE DATA WAS REDUCED WHEN FISH
WERE FED LABELLED PAH. THE RESULTS SUGGEST THAT SIGNIFICANT EXPOSURE
OF FISH TO PAH IS MORE LIKELY TO OCCUR AS A RESULT OF THEIR INGESTING
CONTAMINATED MATERIAL, THAN FROM DIRECT BODY EXPOSURE TO PAH IN THE
WATER COLUMN.
PAGE 20
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iAOOR, C.B.t 8.L. MIOOLEBROOKS, AND B.J. MARTIN. IN PRESS. SEROLOGIC
LTERATIQNS IN CARCINOGEN-EXPOSED TELEOSTS: PROCEDURES FOR PREPARATION AND
NALYSIS OF SAMPLES FROM SMALL FISH. IN: PROCEEDINGS OF THE SYMPOSIUM ON
HE USE OF SMALL FISH SPECIES IN CARCINOGENICITY TESTING, BETHESDA, MARYLAND,
ECEM8ER 8-10, 1981. U.S. NATIONAL CANCER INSTITUTE. CERL.GB X261).
IN ORDER TO STUDY THE EFFECTS OF ENVIRONMENTAL CARCINOGENS ON THE
IMMUNE SYSTEM OF CYPRINQOON VARIEGATUS, IT WAS NECESSARY TO
MINIATURIZE OR MODIFY STANDARD IMMUNOLOGICAL PROCEDURES, DUE TO THE
SMALL SIZE OF THE FISH. MODIFICATIONS IN STANDARD BLEEDING PROCEDURES
ALLOWED COLLECTION OF SUFFICIENT SERUM TO PERFORM MOST SEROLOGICAL
PROCEDURES. SERUM ELECTROPHORESIS SHOWED CONSIDERABLE VARIATON BETWEEN
EXPOSED AND UMEXPOSED FISH AS DID QUALITATIVE IMMUNOELECTROPHORESIS
TECHNIQUES. A 8ACTERIOPHAGE NEUTRALIZATION PROCEDURE WAS SUCCESSFULLY
ADAPTED FOR USE WITH THE C. VARIEGATUS SYSTEM TO MEASURE ANTIVIRAL
ANTIBODIES. THE PRESENCE OF ANTIBODY-FORMING CELLS IN SPLEEN
SUSPENSIONS FROM FISH IMMUNIZED WITH HUMAN TYPE 0 ERYTHROCYTES WAS
DEMONSTRATED BY A MODIFIED IMMUNE ROSETTE PROCEDURE. A CAPILLARY TUBE
PROCEDURE WAS DEVELOPED FOR SEPARATION OF LEUCOCYTES FOM ERYTHROCYTES
IN BLOOD DEVELOPED FOR SEPARATION OF LEUCOCYTES FROM ERYTHROCYTES IN
BLOOD FROM C. VARIEGATUS.
ELIUS, P., 0. ELAN, M. KILGQRE, AND W.P. SCHOOR. 1979. INDUCTION OF
OLYAROMATIC HYDROCARBON OXIOASE IN MARINE ORGANISMS (ABSTRACT). INS
BSTRACTS: XITH INTERNATIONAL CONGRESS OF BIOCHEMISTRY, JULY 8-13, 1979,
ORONTO, CANADA. NATIONAL RESEARCH COUNCIL OF CANADA, TORONTO. PP. 691.
tRL.GB X277>.
CERTAIN KINDS OF DRUGS, SUCH AS PHENOBARBITAL, AND ENVIRONMENTAL
POLLUTANTS, SUCH AS POLYHALOGENATED 8IPHENYLS, ARE WELL-KNOWN TO
INDUCE CYTOCHROME P450 ENZYMES IN RAT LIVER. THE CYTOCHROME P450
ENZYME SYSTEMS ARE RESPONSIBLE FOR SUCH CONVERSIONS AS BENZOPYRENE EPOXIOE. OUR
REPORT IS CONCERNED WITH THE TREATMENT OF MULLET CMUGIL CEPHALUS) AND
KILLIFISH CFUNDALUS GRANDIS) WITH AROCLOR 1254 AT LEVELS FROM 25 TO
200 MG/K. THE INCREASE IN MIXED FUNCTION OXIOASES HAS BEEN MEASURED BY
USING THE AMES TEST ON MICROSOMAL FRACTIONS PREPARED FROM THE FISH
LIVERS WITH BENZOCAiPYRENE, 3-METHYLCHOLANTHRENE AND CRYSENE AS THE
SUBSTRATES. WE HAVE ALSO MEASURED THE BP OXIDASE, THE HEPATIC
MICROSQMAL REOUCTASES AND CYTOCHROME P450 ACTIVITIES IN THE LIVER
MICROSOMAL PREPARATIONS. THE AROCLOR INDUCED LIVERS WERE FOUND TO BE
CAPABLE OF ACTIVATING CERTAIN COMPOUNDS TO MUTAGENS.
PAGE 21
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MELIUS, PAUL. IN PRESS. COMPARATIVE BENZO(A)PYR£N£ METABOLITE PATTERNS IN
FISH AND RODENTS. IN: PROCEEDINGS OF THE SYMPOSIUM ON THE USE OF SMALL FISH
SPECIES IN CARCINQGENICITY TESTING, BETHESDA, MARYLAND, DECEMBER 8-10, 1981.
U.S. NATIONAL CANCER INSTITUTE. CERL,GB X365).
B£NZOPYR£NEDlHYDRQDIOL, 9,10-8ENZOPYREN£ TO POTENT
MUTAGENIC MTEBOLITES AS HAS BEEN DEMONSTATED USING THE AMES TEST,
ESPECIALLY WHEN THE FISH ARE INDUCED WITH AROCLOR OR
3-METHYLCHQLANTHRENE, THE METABOLITE PATTERNS OBTAINED AT DIFFERENT
SUBSTRATE CONCENTRATION LEVELS INDICATE THAT THE METABOLISM IS MORE
COMPLEX AT LOW CONCENTRATIONS WHERE RECYCLING OF METABOLITES IS
PRODUCED IN THE IN VITRO SYSTEM.
MELIUS, PAUL, DAVID ELAM, MELVIN KILGORE, BARRIE TAN, AND W.P. SCHOOR. 1979.
MIXED FUNCTION OXIOASE INDUCIBILITY AND POLYAROMATIC HYDROCARBON METABOLISM
IN THE MULLET, SEA CATFISH, AND GULF KILLIFISH. IN: POLYNUCLEAR AROMATIC
HYDROCARBONS: CHEMISTRY ANO BIOLOGICAL EFFECTS. A. 8JORSETH AND A.J.
DENNIS, EDITORS, BATELLE PRESS, COLUMBUS, OH. PP. 1059-1075. (£RL,6B X268).
OUR STUDIES HAVE SHOWN THAT THE MULLET (MUGIL CEPHALUS), THE SEA
CATFISH BAP IS A LESS EFFECTIVE INOUCER
THAT 30-MC OR (2) IN VIVO BAP METABOLITE MAY HAVE INDUCED THE
CONJUGATION SYSTEMS, WHICH WOULD RESULT IN ETHYL ACETATE INSOLUBLE ANO
NONMUTAGENIC IN VITRO BAP METABOLITES. THESE STUDIES SHOW THAT CERTAIN
SIMILARITIES EXIST IN THE MECHANICS OF FISH AND MAMMALIAN NFO SYSTEMS
EVEN THOUGH DIFFERENCES EXIST IN THE ACTIVITIES OF THESE SYSTEMS.
CHAMBERS ANO YARBROUGH SPECULATE THAT THE LESS ACTIVE MFO SYSTEMS OF
FISH REFLECT THEIR EVOLUTIONARY ADAPTATION TO AN ENVIRONMENT IN WHICH
XENOBIOTICS ARE NATURALLY DILUTED.
MEYERS, T.R., ANO J.D. HENDRICKS. 1983. HISTOPATHOLOGY OF FOUR SPONTANEOUS
NEOPLASMS IN THREE SPECIES OF SALMONID FISHES. J. FISH DIS. 6<5>:481-499.
<£RL,GB X358).
GROSS AND HISTOLOGICAL DESCRIPTIONS OF FOUR DIFFERENT SPONTANEOUS
NEOPLASMS IN THREE SPECIES OF SALMONIO FISHES ARE PROVIDED: THYMIC
LYMPHOMA AND DERMAL FIBROSARCOMA, RESPECTIVELY, IN TWO ARTIFICIALLY
REARED SOCKEYE SALMON, ONCORHYNCHUS NERKA CWAL8AUM), RENAL
PAPILLIFEROUS CYSTADENOMA IN A WILD CAUGHT CHINOOK SALMON, 0.
TSHAWYTSCHA
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MEYERS, THEODORE «., AND JERRY 0. HENDRICKS. 1982. SUMMARY OF TISSUE
LESIONS IN AQUATIC ANIMALS INDUCED BY CONTROLLED EXPOSURES TO ENVIRONMENTAL
CONTAMINANTS. CHEMOTHERAPEUTIC AGENTS, ANO POTENTIAL CARCINOGENS. MAR.
FISHERIES REV. 44M-17. (ERL.GB X368>.
THIS ARTICLE WAS WRITTEN TO PROVIDE A USEFUL REFERENCE FOR AQUATIC
TOXICOLOGISTS/PATHOLOGISTS BY CONSOLIDATING THIS DESCRIPTIVE MATERIAL
WITH KEY REFERENCES THROUGH 1981. THIS INFORMATION IS PRESENTED IN
FIVE TABLES. TABLE 1 IDENTIFIES AT LEAST 90 ENVIRONMENTAL CONTAMINANTS
USED AS PESTICIDES, HERBICIDES, OR AS INDUSTRIAL CHEMICALS WHICH ARE
CATEGORIZED IN THE FOLLOWING MANNER: ORGANOCHLORINES, PETROLEUM
COMPOUNDS* ORGANOPHOSPHATES, CARSAMATES, HEAVY METAL SALTS,
NITROGENOUS COMPOUNDS, MISCELLANEOUS COMPOUNDS, AND CHEMOTHERAPEUTIC
AGENTS. TABLE 4 LISTS AT LEAST 50 COMPOUNDS GROUPED AS MYCOTOXINS,
PLANT DERIVATIVES, AND OTHER CHEMICALS ALL OF WHICH HAVE BEEN TESTED
FOR CARCINOGENIC ACTIVITY. TABLES 2, 3 AND 5 DESCRIBE AND REFERENCE
MISTQLQGIC AND/OR ULTRASTRUCTURAL LESIONS OR ABNORMALITIES CAUSED BY
THESE COMPOUNDS, THEIR ROUTES OF EXPOSURE, AND AQUATIC SPECIES TESTED.
MIX, M.C. 1983. HAEMIC NEOPLASMS OF BAY MUSSELS, MYTILUS EOULIS, FROM
OREGON: OCCURRENCE, PREVALENCE, SEASONALITY, AND HISTOPATHOLOGICAL
PROGRESSION. J. FISH DIS. 6C3):239-248. (6RL.GB X3T9).
THE OCCURRENCE, PREVALENCE, SEASONALITY ANO HI STOPATHQLOGICAL
PROGRESSION OF A CELLULAR DISORDER, THOUGHT TO BE A HAEMIC NEOPLASM,
WERE STUDIED IN SUBPOPULATIONS OF MYTILUS EDULIS INHABITING DIFFERENT
SITES IN YAQUINA BAY. OREGON, FROM 1976-1981. THERE WERE SIGNIFICANT
DIFFERENCES IN THE OCCURRENCE OF THE DISORDER THAT WERE RELATED TO
GEOGRAPHICAL LOCATION. IN THE SUBPOPULATION WITH THE HIGHEST LEVELS OF
THE DISEASE, THE PREVALENCE RATES RANGED FROM 0 TO 20t WITH A 5-YEAR
MEAN OF 9,8*. THERE WAS A STATISTIC IALLY SIGNIFICANT RELATIONSHIP
BETWEEN PREVALENCE ANO SEASON. DURING THE 5-YEAR STUDY PERIOD, THERE
WAS A CONSISTENT PATTERN CHARACTERIZED BY HIGHEST PREVALENCES DURING
JANUARY TO MARCH FOLLOWED BY A PERIOD OF DECLINE TO LOWER LEVELS
DURING THE SUMMER ANO EARLY AUTUMN, AFTER WHICH THERE WAS AN INCREASE.
DATA ANALYSES REVEALED THAT THERE WAS NO SEASONAL HISTOPATHOLOGICAL
PROGRESSION OF THE DISORDER. NUMBERS OF STAGE 1 CEARLY), 2, 3 ANO 4
CADVANCED) CASES WERE NOT RELATED TO SEASON BUT OCCURRED IN A RANDOM
MANNER THROUGHOUT THE ENTIRE YEAR.
PAGE 23
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MIX, MICHAEL C. 1983. STUDIES ON POLYNUCLEAR AROMATIC HYDROCARBONS AND
METALS IN MOLLUSCS (ABSTRACT). IN: WORKSHOP ON SU8LETHAL EFFECTS OF STRESS
ON MARINE ORGANISMSt MARCH 30-31, 1982, ASILOMAR, PACIFIC GROVE, CALIFORNIA.
MICHAEL MARTIN AND FLORENCE HARRISON, EDITORS, NTIS, SPRINGFIELD, VA. PP.
55. CERL.GB X283).
DURING THE PAST 5 YEARS, OUR RESEARCH HAS FOCUSED ON MEASURING LEVELS
OF 15 UNSU3STITUTED POLYNUCLEAR AROMATIC HYDROCARBONS (PNAH) AND
CERTAIN METALS IN BIVALVE MOLLUSCS FROM INDIGENOUS POPULATIONS THAT
INHABIT OREGON ESTUARIES. THE PRIMARY OBJECTIVES OF SOME OF THE MAJOR
STUDIES WERE TO: CD MEASURE CONCENTRATIONS OF PNAH AND METALS IN
SHELL FISH FROM DIFFERENT LOCATIONS FOR AN EXTENDED PERIOD OF TIME IN
ORDER TO ESTABLISH A DEPENDABLE BASELINE, (2) DETERMINE WHETHER OR NOT
PNAH CONCENTRATIONS IN MOLLUSCS REFLECT THE DEGREE OF ENVIRONMENTAL
CONTAMINATION, (3) EVALUATE SEASONAL VARIATIONS IN PNAH AND METAL
CONCENTRATIONS IN MOLLUSCS, (4) DETERMINE WHETHER OR NOT THERE HERE
STATISTICAL CORRELATIONS BETWEEN THE PRESENCE OF CELLULAR
PROLIFERATIVE DISORDERS IN SUBPQPULATIQNS OF MUSSELS (MYTILUS EOULIS)
AND PNAH CONCENTRATIONS IN THEIR TISSUES, AND (5) DETERMINE WHETHER OR
NOT CERTAIN STATISTICAL RELATIONSHIPS COULO BE USED FOR PREDICTIVE
PURPOSES IN DETERMINING AND EVALUATING PNAH CONCENTRATIONS IN
INDIGENOUS SHELLFISH. OUR DATA SUGGEST THAT A TWO OR THREE YEAR PERIOD
IS REQUIRED TO ESTABLISH BASELINE LEVELS OF PNAH IN SHELLFISH. THERE
WERE SIGNIFICANT CORRELATIONS BETWEEN PNAH CONCENTRATIONS IN MUSSELS,
THE OCCURRENCE OF CELLULAR DISORDERS AND THE QUALITY OF THE
ENVIRONMENT THEY INHABIT. THERE WERE SIGNIFICANT SEASONAL VARIATIONS
IN BOTH PNAH AND METAL CONCENTRATIONS IN M. EDULIS. FINALLY, THE DATA
FROM OUR STUDIES INDICATE THAT IT MAY BE POSSIBLE TO IDENTIFY
SITE-SPECIFIC VARIABLES (INDIVIDUAL PNAH OR METALS) THAT CAN BE USED
FOR ASSESSING PNAH CONCENTRATION IN THE ENVIRONMENT.
MIX, MICHAEL C. 1979. CHEMICAL CARCINOGENS IN BIVALVE MOLLUSKS FROM OREGON
ESTUARIES. EPA-600/3-79-034, U.S. ENVIRONMENTAL PROTECTION AGENCY,
ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, FL. 33P.
THE RESEARCH UNDERTAKEN INVOLVED THE USE OF INDIGENOUS POPULATIONS OF
BIVALVE MOLLUSKS AS MONITORS FOR DETECTING AND QUANTIFYING
ENVIRONMENTAL BENZO(A)PYRENE
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MIXf MICHAEL C. 1982. PQLYNUCLEAR AROMATIC HYDROCARBONS AND CELLULAR
PROLIFERATIVE DISORDERS IN BIVALVE MOLLUSCS FROM OREGON ESTUARIES.
EPA-600/4-82-026, U.S. ENVIRONMENTAL PROTECTION AGENCY, ENVIRONMENTAL
RESEARCH LABORATORY, GULF BREEZE, FL. 49P.
THE RESEARCH PROJECT INVOLVED UTILIZING INDIGENOUS POPULATIONS OF
ECONOMICALLY-IMPORTANT BIVALVE MOLLUSCS AS MONITORS FOR DETECTING AND
QUANTIFYING ENVIRONMENTAL PNAH, INCLUDING 11 COMPOUNDS CLASSIFIED AS
CARCINOGENS, 11 EPA PRIORITY POLLUTANTS AND 11 TOXIC POLLUTANTS,
8ASELINE LEVELS OF PNAH WERE DETERMINED DURING A TWO-YEAR PERIOD FOR
MUSSELS CM. EDULIS), CLAMS CM. ARENARIA AND T. CAPAX) AND OYSTERS FROM DIFFERENT SITES, RANGING FROM RELATIVELY PRISTINE TO
MODERATELY POLLUTED, IN YAQUINA, COOS AND TILLAMOOK BAYS, OREGON.
TOTAL CONCENTRATIONS OF 15 UNSUBSTITUTSO PNAH WERE 30-60 UG/KG IN
SHELLFISH FROM UNCONTAMINATED WATERS TO GREATER THAN 1000 UG/KG IN
THOSE FROM SITES CLASSIFIED AS CONTAMINATED. A MAJOR EFFORT WAS MADE
TO DETERMINE AND EVALUATE CERTAIN RELATIONSHIPS BETWEEN PNAH AND THEIR
CONCENTRATIONS IN SHELLFISH. STUDIES WERE CONDUCTED TO: DETERMINE THE
EFFECTS OF DEPURATION ON PNAH CONCENTRATIONS; IDENTIFY SEASONAL
DIFFERENCES IN PNAH CONCENTRATIONS; AND MEASURE BAP UPTAKE AND
ELIMINATION. PRELIMINARY STUDIES INDICATED THAT MUSSELS MAY POSSESS A
LIMITED ABILITY TO METABOLIZE BAP. MULTIPLE REGRESSION AND MULTIPLE
CORRELATION TECHNIQUES WERE USED TO IDENTIFY AND EVALUATE
INTERRELATIONSHIPS BETWEEN PNAH. CERTAIN RELATIONSHIPS MAY BE USEFUL
FOR PREDICTIVE PURPOSES IN EVALUATING ENVIRONMENTAL PNAH. THE DATA
FROM THESE STUDIES INDICATE THAT IT MAY SE POSSIBLE TO IDENTIFY
SITE-SPECIFIC, SIGNIFICANT VARIABLES (INDIVIDUAL PNAH) AFTER A
SUITABLE PERIOD OF SAMPLING AND TO SUBSEQUENTLY MEASURE ONLY THOSE KEY
VARIABLES FOR AN ADEQUATE ASSESSMENT OF TOTAL PNAH. COMBINED WITH
OTHER APPROACHES, THIS MAY RESULT IN CONSIDERABLE COST REDUCTIONS FOR
LONG-TERM BIOLOGICAL MONITORING PROGRAMS. CELLULAR PROLIFERATIVE
DISORDERS, RESEMBLING NEOPLASTIC CONDITIONS IN VERTEBRATES, WERE FOUND
IN MUSSELS WITH THE GREATEST PNAH CONCENTRATIONS. FURTHER STUDIES WILL
BE NECESSARY TO DETERMINE THE SIGNIFICANCE OF THIS CORRELATION. THIS
REPORT WAS SUBMITTED IN FULFILLMENT OF CONTRACT NO. R806224020 BY
OREGON STATE UNIVERSITY, CORVALLIS, OREGON, UNDER THE SPONSORSHIP OF
THE U.S. ENVIRONMENTAL PROTECTION AGENCY. THIS REPORT COVERS THE
PERIOD OCTOBER 1, 1978 TO NOVEMBER 30, 1980.
MIX, MICHAEL C*, AND WILBUR P. BREESE. 1980. CELLULAR PROLIFERATIVE
DISORDER IN OYSTERS COSTREA CHILENSIS) FROM CHILOE, CHILE, SOUTH AMERICA.
INVERTEBR. PATHOL. 36(1):123-12*. CERL.GB X252).
BEGINNING IN THE LATE 1960S AND THROUGH-OUT THE PAST DECADE, UNUSUAL,
PERHAPS NEOPLASTIC, LARGE CELLS WITH RATHER CONSISTENT CHARACTERISTICS
WERE DESCRIBED IN MUSSELS, CLAM, AND OYSTERS FROM SEVERAL BAYS IN THE
UNITED STATES AND THE UNITED KINGDOM CM.C. MIX, J.W. HAWKES, AND A.K.
SPARKS* J. INVERTEBR. PATHOL. 34, 41-56, 1979> THE LARGE,
CHARACTERISTIC CELLS ARE THOUGHT BY MANY WORKERS TO BE ABNORMAL
LEUKOCYTES OR LEUKOCYTE PRECURSORS CE.G., C.A. FARLEY AND A.K. SPARKS,
BIBL. HAEMATOL. 36, 610-617, 1970). THIS NOTE DESCRIBES AN APPARENTLY
IDENTICAL CONDITION IN OSTREA CHILENSIS FROM CHILE.
PAGE 25
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MIX, MICHAEL C.t DIANE L. BUNTING, AND D.T. A8BOTT. 1979. PRELIMINARY
STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARVAL STAGES OF THE
GOOSE BARNACLEt POLLICIPES POLYMERUS FOR MARINE BIOASSAYS. IN: PROCEEDINGS
OF THE SECOND BIENNIAL CRUSTACEAN HEALTH WORKSHOP, TAMU-SG-79-114, D.H.
LEWIS AND J.K. LEONG, EDITORS, TEXAS A £ M UNIVERSITY, COLLEGE STATION, TX,
PP. 361-381. CERLtGB X166).
THE GENERAL PURPOSE OF THESE STUDIES WAS TO DETERMINE IF THE
DEVELOPMENTAL AND LARVAL STAGES OF THE GOOSE BARNACLE, POLLICIPES
POLYMERUS WOULD BE USEFUL ORGANISMS TO USE AS A MARINE BIOASSAY
SYSTEM. DIFFERENT CULTURE SYSTEMS AND CULTURING CONDITIONS WERE
DEVELOPED AND EVALUATED. WE DESIGNED A SIMPLE, INEXPENSIVE SYSTEM THAT
CAN BE USED TO CULTURE FERTILE EGGS UNDER NORMAL LABORATORY CONDITIONS
(ROOM TEMPERATURE AND AMBIENT LIGHT-DARK CYCLE). THE CULTURE MEDIUM
CONSISTS OF INSTANT OCEAN SEA SALTS MIXED IN TAP WATER TO A SALINITY
OF 28-30 PARTS PER THOUSAND. ANTIBIOTIC CONCENTRATIONS OF 10 MG/L
SEAUATER OF STREPTOMYCIN SULFATE AND 10 MG.L SEAWATER OF PENICILLIN G
ARE THEN ADDED TO THE ARTIFICIAL SEAWATER. AS A RESULT OF THESE AND
OTHER STUDIES, IT SEEMS LIKELY THAT P. PQLYMERUS MAY BE A VALUABLE
6IOASSAY ORGANISM. MORE COMPLETE STUDIES, NECESSARY TO GATHER LARGE
AMOUNTS OF DATA FOR DEVELOPINGAND STATISTICALLY EVALUATING DIFFERENT
CRITERIA TO DETERMINE DELETERIOUS EFFECTS, ARE REQUIRED BEFORE A FINAL
JUDGEMENT CAN BE MADE ABOUT THE FUTURE USE OF P. POLYMERUS FOR
BIOASSAYS.
MIX, MICHAEL C.t JOYCE W. HAWKES, AND ALBERT K. SPARKS. 1979. OBSERVATIONS
ON THE ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTATIVE NEOPLASTIC
DISORDERS OF MUSSELS, MYTILUS EOULIS, FROM YAQUINA BAY, OREGON. J.
INVERTEBR. PATHOL. 34Cl):41-56.
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MIX, MICHAEL C.t SUSAN J. HcMINGWAY, AND RANDY I. SCHAFFER. 1982.
8ENZO(A)PYREN£ CONCENTRATIONS IN SOMATIC AND GQNAD TISSUES OF BAY MUSSELS*
MYTILUS EOULIS. 8ULL. ENVIRON. CONTAM. TOXICOL. 28
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MIXt MICHAEL C., AND RANDY L. SCHAFFER. 1983. CONCENTRATIONS OF
UNSUBSTITUTEO POLYCYCLIC AROMATIC HYDROCARBONS IN SOFTSHELL CLAMS FROM COOS
BAY, OREGON* USA. MAR. POLLUT. BULL. l4(3>:94-97, (ERL,GB X338).
CONCENTRATIONS OF B£NZOCA)PYRENE COMPARED TO 76.3 UG KGC-1) FOR
CLAMS FROM A MORE REMOTE ENVIRONMENT. IN GENERAL, PNAH CONCENTRATIONS
WERE LOWEST IN THE FALL-WINTER AND HIGHEST DURING THE SPRING-SUMMER.
MIX, MICHAEL C., AND RANDY L. SCHAFFER. 1983. CONCENTRATIONS OF
UNSUBSTITUTEO POLYNUCLEAR AROMATIC HYDROCARBONS IN BAY MUSSELS CMVTILUS
EDULIS) FROM OREGON, USA. MAR. ENVIRON. RES. 9<4):193-209. <£RL,G8 X397>.
CONCENTRATION OF FIFTEEN UNSUBSTITUTED POLYNUCLEAR AROMATIC
HYDROCARBONS (PNAH) WERE MEASURED IN MYTILUS EDULIS FROM TWO SITES IN
YAQUINA BAY, OREGON, USA, DURING 1979-1980. THERE WERE SIGNIFICANT
DIFFERENCES IN PNAH LEVELS BETWEEN THE TWO POPULATIONS. THE AVERAGE
TOTAL CONCENTRATION IN MUSSELS INHABITING THE MORE INDUSTRIALIZED
BAYFRONT WAS 986 X 2 UG/KG COMPARED WITH 273 X 9 UG/KG IN MUSSELS FROM
A MORE REMOTE SITE ACROSS THE SAY. SUBSTANTIAL DIFFERENCES WERE FOUND
IN THE CONCENTRATIONS OF DIFFERENT PNAH IN M. EOULIS EXAMINED DURING
THIS STUDY. THE SMALLER, MORE WATER SOLUBLE, COMPOUNDS WERE
CONCENTRATED TO ONE OR TWO ORDERS OF MAGNITUDE ABOVE THE LARGER, LESS
SOLUBLE PNAH.
MIX, MICHAEL C., RANDY L. SCHAFFER, AND SUSAN J. HEMINGWAY. 1981.
POLYNUCLEAR AROMATIC HYDROCARBONS IN BIVALVE MOLLUSKS IN BAY MUSSELS CMYTILUS
EDULIS) FROM OREGON. IN: PHYLETIC APPROACHES TO CANCER: PROCEEDINGS OF THE
11TH INTERNATIONAL SYMPOSIUM OF THE PRINCESS TAKAMATSU CANCER RESEARCH FUND,
TOKYO, 1980. CLYDE J. DAWE, EDITOR, JAPAN SCI. SOC. PRESS, TOKYO. PP.
167-177.
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MIX, MICHAEL C., RANDY L. SCHftFFER, AND DAVID Y. LATOUCHE. 1980.
ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL
CONDITIONS (ABSTRACT). IN: PROCEEDINGS OF THE ANNUAL MEETING OF THE SOCIETY
OF INVERTEBRATE PATHOLOGY. <£RL,GB X259).
DURING THE PAST DECAOEt THERE MERE SEVERAL REPORTS OF
HISTOPATHOLOGICAL DISORDERS IN 9IVALVE MOLLUSKS THAT WERE TENTATIVELY
LINKED WITH INCREASED LEVELS OF ENVIRONMENTAL POLLUTANTS, WERE
ANALYZED USING REVERSE SOLUTION. MORE RECENTLY, "MUSSEL WATCH"
PROGRAMS HAVE UTILIZED SHELLFISH AS BIOMONITORS FOR MEASURING LEVELS
OF SPECIFIC ENVIRONMENTAL CONTAMINANTS. OUR RESEARCH HAS BEEN
CONCERNED WITH A POSSIBLE CORRELATION BETWEEN BODY BURDENS OF
CARCINOGENIC POLYNULCEAR AROMATIC HYDROCARBONS (PNAH) AND
PROLIFERATIVE DISORDERS IN M. EOULIS ANO OTHER BIVALVE SHELLFISH. WE
HAVE RECENTLY EXTENDED OUR STUDIES TO INCLUDE METALS ANO HAVE ALSO
INITIATED STUDIES DESIGNED TO TEST THE VALIDITY OF CERTAIN ASSUMPTIONS
ANO PRINCIPLES GENERATED FROM EARLIER MONITORING STUDIES. EXTRACTS OF
MUSSELSt CLAMS AND SEDIMENTS FROM OREGON ESTUARIES WERE ANALYZED FOR
PNAH BY METHODS DEVELOPED IN OUR LABORATORY (A MODIFICATION OF DUNN'S
TECHNIQUES). SAMPLES WERE PREPARED BY BASIC DIGESTION, LIQUID-LIQUID
EXTRACTION, COLUMN CHROMATGGRAPHY ON FLORISIL, OMSO EXTRACTION AND
COLUMN CHROMATOGRAPHY ON SEPHAOEX LH-20 PRIOR TO ASSAY. EIGHTEEN PNAH,
INCLUDING 12 EPA PRIORITY POLLUTANTS, WERE ANALYZED USING REVERSE
PHASE LIQUID CHROMATOGRAPHY (HPLC) WITH UV AND FLUORESCENT DETECTION.
LEVELS OF MN, NI, CU, ZN, CD, U ANO AL WERE DETERMINED WITH FLAME
ATOMIC ABSORPTION ANO/OR NUETRQN ACTIVATION ANALYSIS. WE HAVE
IDENTIFIED POTENTIAL RELATIONSHIPS BETWEEN PATHOLOGICAL CONDITIONS AND
CONCENTRATIONS OF POLLUTANTS IN BIVALVE MOLLUSKS. WE HAVE ALSO FOUND
THAT FLAWS IN CERTAIN ASSUMPTIONS USED IN OTHER STUDIES WILL
NECESSITATE A CRITICAL EXAMINATION OF MONITORING PROCEDURES.
MIX, MICHAEL C«, STEVE R. TRENHOLM, AND KEITH I. KING. 1979. BENZO
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MORENO, MARK, AND B.J. MARTIN. 1979. EM8RYQLOGIC DEVELOPMENT OF THE
SHEEPSHEAD MINNOW (CYPRINOOON VARIEGATUS) (ABSTRACT). J. MISS. ACAD. SCI,
XXIVSCSUPPLEMENT116. (ERL,GB X176).
SEXUALLY MATURE SPECIMENS COLLECTED FROM THE MISSISSIPPI GULF COAST
WERE MAINTAINED IN AQUARIA AT 15-20% SALINITY AND 30 DEGREES CELSIUS.
THE PHOTOPERIOO WAS MAINTAINED AT 14 HOURS LIGHT AND 10 HOURS DARK
THROUGHOUT THE EXPERIMENT. EGGS WERE OBTAINED BY TEMPERATURE INDUCED,
OR HUMAN CHORIQNIC GONAOOTROPIM INDUCED SPAWNING. DATA INCLUDE
MECHANISMS OF: FERTILIZATION, 5LASTULATION, GASTRULATION, SPIBOLY,
AND ORGANOGENESIS. THIS STUDY SUGGESTS A POSSIBLE SIGNIFICANCE OF
CERTAIN "WEDGE-LIKE" CELLULAR STRUCTURES SEEN IN EARLY CLEAVAGE.
PATTONt JOHN S., AND JOHN A. COUCH. IN PRESS. CAN TISSUE ANOMALIES THAT
OCCUR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT CHEMICALS?. IN:
PROCEEDINGS OF CONFERENCE ON MEANINGFUL MEASURES OF MARINE POLLUTION EFFECTS*
APRIL 26-29, 1982, PENSACOLA BEACH, FL. U.S. NATIONAL OCEANIC AND
ATMOSPHERIC ADMINISTRATION. CERLtGS 474).
THE ADVANTAGE OF USING TISSUE ABNORMALITIES IN WILD FISH AS A MEASURE
OF FISH HEALTH IS THAT THE ABNORMALITY, UNLIKE SENSITIVE BIOCHEMICAL
ANOMALIES, CANNOT BE SAID TO HAVE OCCURRED DURING CAPTURE OR TRANSPORT
TO THE LAB. IT USUALLY TAKES HOURS, DAYS, WEEKS, AND SOMETIMES EVEN
MONTHS FOR ABNORMAL TISSUE PATHOLOGIES TO DEVELOP. THE RESEARCHER CAN
BE CONFIDENT THAT SOME FACTOR IN THE FIELD CAUSED THE ABORMALITY. WHEN
AN ABNORMAL FISH IS CAPTURED, LOGICAL QUESTIONS APPEAR AT FOUR
DIFFERENT LEVELS: 1) WHAT IS THE STRUCTURE OF MORPHOLOGY OF THE
ABNORMALITY? MANY SCHOLARLY ARTICLES BY HISTOPATHQLOGISTS DESCRIBE IN
DETAIL TISSUE ANOMALIES OBSERVED IN FIELD SPECIMENS. AT THIS LEVEL THE
FOCUS IS ON THE PATHOLOGY ITSELF. 2) WHAT IS THE INCIDENCE OF THE
PATHOLOGY IN THE POPULATION? HOW MANY ARE AFFLICTED, OLD OR YOUNG,
MALE OR FEMALE? HERE THE FOCUS IS ON THE SPECIES POPULATION. 3) CAN
THE INCIDENCE OF A FISH DISEASE BE LINKED TO ENVIRONMENTAL POLLUTION?
HERE THE FOCUS IS ON CORRELATING PATHOLOGY WITH POLLUTION. 4) WHAT
FACTOR OR FACTORS
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PORTERt RONALD C., AND 6.J. MARTIN. 1980. HISTOLOGY OF THE POST-PHARYNGEAL
DIGESTIVE TRACT OF THE SHEEPSHSAD MINNOW, CYPRINODON VARIEGATUS CA8STRACT).
J. MISS. ACAO. SCI. 25.
IT HAS BEEN DEMONSTRATED THAT THE CARBON FLUX IN BIVALVES IS
STIMULATED BY LOW LEVELS OF NAPHTHALENE. THE EFFECT OF NAPHTHALENE ON
THE GLUCOSE TRANSPORT SYSTEM WAS STUDIED UTILIZING
C14-ALPHA-METHYLGLUCOSIOE CMGP) ANO H3-INSULIN. MGP TRANSPORT FOLLOWS
MICHAELIS-MENTON KINETICS ANO IS COMPETIVELY INHIBITED BY
ALPHA-0-GLUCOSE. DINITROPHENOL, PENTACHLOROPHENOL, CARBONYl-CYANIOE
ANO DICYCLOHEXYLCARBODIIMIDE ALL INHIBIT TRANSPORT. QUABAIN AND
IODOACETATE HAVE NO EFFECT. NAPHTHALENE STIMULATES TRANSPORT.
INHIBITORS STIMULATE OIFFUSIONAL LEAKAGE OF MGP. TRANSPORT SYSTEMS IN
THE GILL MAY FUNCTION TO MAINTAIN INTRACElLULAR OSMOTIC BALANCE. THE
MECHANISM BY WHICH NAPHTHALENE STIMULATES GLUCOSE TRANSPORT IS NOT
KNOWN. HOWEVER, IT IS HYPOTHESIZED THAT THE STIMULATORY EFFECT IS A
RESULT OF ALTERATIONS IN LIPIO-PROTEIN INTERACTIONS WITHIN THE LIPID
8ILAYER. A COROLLARY TO THIS IS THAT AT HIGHER TEMPERATURE THE
MEMBRANE 'WILL BECOME LEAKY DUE TO INCREASED DISORDER OF LIPID PACKING.
WHEN LEAKAGE BECOMES GREAT THE PART OF MGP ACCUMULATION WILL DROP.
NAPHTHALENE LOADING SHOULD REDUCE THE TEMPERATURE AT WHICH LEAKAGE
BEGINS. THE EFFECT OF TEMPERATURE ON MGP LEAKAGE IN NAPHTHALENE LOADED
GILLS IS CURRENTLY BEING INVESTIGATED.
RILEYt R.T., AND M.C. MIX. 1981. EFFECTS OF NAPHTHALENE ON GLUCOSE
METABOLISM IN THE EUROPEAN FLAT OYSTER, QSTREA EOULIS. COMP. BIQCHEM.
PMYSIOL. C COMP. PHARMACOL. 70C(1):13-20. CERLtGB X256).
(1). THE POOL SIZES AND REDISTRIBUTION OF C-14 LABEL FROM
DCU-C-1O6LUCOSE INTO ETHANOL INSOLUBLE POLYSACCHARIDES, TOTAL
PROTEIN, TOTAL POLAR LIPIDS, TOTAL NEUTRAL LIPIDS, FREE ALANINE,
ASPARTATE, GLUTAMATE AND TOTAL ORGANIC ACIDS WERE DETERMINED FOR
CONTROL AND NAPHTHALENE-TREATED OYSTERS OSTREA EDULIS. C2). THE
FOLLOWING EFFECTS WERE ATTRIBUTED TO NAPHTHALENE TREATMENT: A
DECREASE IN THE PERCENTAGE OF PROTEIN ANO POLAR LIPIO: AN INCREASE IN
THE TOTAL AMINO ACIDS CSUM DF ALANINE, ASPARTATE AND GLUTAMATE); AN
INCREASE ZN ORGANIC ACIDS! AND INCREASE IN THE MEAN SPECIFIC ACTIVITY
(GEOMETRIC) FOR ALL MEASURED POOLS. (3). THESE RESULTS SUGGESTED THAT
NAPHTHALENE STIMULATED PROTEIN AND POLAR LIPIO CATABOLISM, WHILE
SIMUTANEOUSLY STIMULATING THE FLOW OF GLUCOSE-CARBON INTO ALL MEASURED
POOLS.
PAGE 31
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RILEY, R.T.t M.C. MIX, R.L. SCHAFFER, AND O.L. BUNTING. 1981. UPTAKE AND
ACCUMULATION OF NAPHTHALENE 8Y THE OYSTER QSTREA EOULISt IN A FLOW THROUGH
SYSTEM. MAR. 8IOL. 61(45:267-276. (ERL,G8 X255).
A FLOW-THROUGH SYSTEM WAS USED TO FOLLOW NAPHTHALENE AND NAPHTHALENE
METABOLITE ACCUMULATION IN THE SEAWATER AND IN THE TISSUE OF THE
OYSTER OSTREA EOULIS. AFTER 72 H, 82.5% OF THE NAPHTHALENE CARBON HAS
RECOVERED FROM THE SYSTEM. GLUCOSE WAS ADDED TO SEAWATER TO STIMULATE
THE PATHWAYS OF GLUCOSE METABOLISM IN THE OYSTERS. STREPTOMYCIN C100
PPM> REDUCED MICROBIAL OXIDATION OF NAPHTHALENE AND GLUCOSE, AND
REDUCED BACTERIAL GROWTH. HOWEVER, EVEN IN THE PRESENCE OF
STREPTOMYCIN, MICROBIAL OXIDATION OF NAPHTHALENE WAS CONSIDERABLE. THE
MAIN OXIDATION PRODUCT RECOVERED FROM SEAWATER WAS 14C02.
RADIOACTIVITY WAS ALSO ASSOCIATED WITH COMPOUNDS WHICH SEPARATED BY
TLC WITH 2- AND 1- NAPHTHOL. THE PATTERN OF NAPHTHALENE UPTAKE AND
ACCUMULATION IN OYSTER TISSUES WAS RELATIVELY CONSTANT AFTER ONLY A
FEW HOURS OF EXPOSURE TO NAPHTHALENE. THE POTENTIAL OF TISSUES TO
ACCUMULATE NAPHTHALENE WAS SHOWN TO BE A FUNCTION OF MULTIPLE
VARIABLES SUCH AS NUTRITIONAL STATE, LIPIO CONCENTRATION, LENGTH OF
EXPOSURE TO NAPHTHALENE, AND THE EXTERNAL NAPHTHALENE CONCENTRATION.
CARBON-14-LABELEO METABOLITES DERIVED FROM 14C-NAPHTHALENE WERE
CONSISTENTLY RECOVERED FROM DIGESTS OF THE OYSTER TISSUES. NON-C02
ALKALINE-SOLUBLE SUBSTANCES WERE THE PRIMARY METABOLITES.
HEXANE-EXTRACTABLE SUBSTANCES* WHICH SEPARATED BY TLC WITH KNOWN
STANDARDS OF 2- AND 1- NAPHTHOL, WERE CONSISTENTLY RECOVERED FROM
SEAWATER AND TISSUE DIGESTS. IT WAS NOT POSSIBLE TO CONCLUDE THAT
THESE METABOLITES WERE A RESULT OF NAPHTHALENE METABOLISM BY OYSTER
ENZYME SYSTEMS.
RILEY* RONALD T., AND MICHAEL C. MIX. 1980. THIN-LAYER SEPARATION OF CITRIC
ACIO CYCLE INTERMEDIATES, LACTIC ACIO, AND THE AMINO ACID TAURINE. J.
CHROMATOGR. 189<2):286-288. . ALSO, SAMPLE
REMOVAL FROM SILICA GEL LAYERS CAN BE EXCEEDINGLY TEDIOUS. THIN-LAYER
SEPARATION METHODS HAVE ALSO BEEN DESCRIBED WHICH MADE USE OF SILICA
GEL-CELLULOSE MIXED LAYERS (2,3,6). THIS PAPER DESCRIBES A
TWO-DIMENSIONAL MIXED-LAYER METHOD FOR SEPARATING CITRIC ACID CYCLE
INTERMEDIATES, LACTIC ACID AND THE AMINO ACID TAURINE. THE METHOD
CLEANLY SEPARATES ALL CITRIC ACIO CYCLE INTERMEDIATES TESTED*
EXCEPTING CITRIC ACIO AND ISOCITRIC ACIO. THE SOLVENTS ARE INEXPENSIVE
AND THE METHOD ALLOWS FOR THE RAPID AND QUANTITATIVE REMOVAL OF
ISOLATED 14C-LABELED ACIDS BY UTILIZING A SIMPLE CELLULOSE ACETATE
STRIPPING MIXTURE(T).
PAGE 32
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RILEY, RONALD T.f AND MICHAEL C. MIX. 1981. ION-EXCHANGE TECHNIQUE FOR
CONCENTRATING AMMONIA FROM SMALL VOLUMES OF SEAWATER. MAR. CHEM,
10(2):i59-160. CERL.GB X205).
AN ION-EXCHANGE TECHNIQUE HAS USED TO CONCENTRATE AMMONIA FROM
SiAWATER. PRECIPITATION OF BIVALENT CATIOMSi PRIOR TO CONCENTRATIONt
REDUCED THE VARIABILITY OF AMMONIA RECOVERY FROM THE ION-EXCHANGE
COLUMN AND LENGTHENED COLUMN LIFE. ANALYSIS OF THE ELUATE WAS BY THE
PHENOLHYPOCHLORITE REACTION. THE METHOD MAS DESIGNED FOR USE WITH
SMALL SEAWATER SAMPLES 8399-40*. (ERL,GB X350).
TREATMENT OF YERSINIA RUCKERI-INFECTEO JUVENILE COHO SALMON WITH
TOBRAMYCIN REVEALED SIGNIFICANT TOXICITV OF THE DRUG TO THESE
FISH. IN A GROUP OF FISH RECEIVING ONLY 5 MG/KG/DAV A CUMULATIVE
MORTALITY OF 100* WAS OBSERVED IN 21 DAYS. TO VERIFY THE SITE OF
TOXICITY* A SECOND STUDY WAS CONDUCTED IN ORDER TO HISTOLOGICALLY
EVALUATE SPECIFIC TISSUES AND TO DETERMINE DOSE-RESPONSE
CHARACTERISTICS. TBM PRODUCED NEPHOTOXICITY IN JUVENILE COHO SALMON AT
DOSAGES ONLY HALF AS GREAT AS THOSE REQUIRED FOR AN EQUIVALENT EFFECT
IN MAMMALS AND DID SO OVER A MUCH SHORTER TREATMENT COURSE.
HISTOLOGICAL EXAMINATION REVEALED MODERATE TO COMPLETE PROXIMAL TUBULE
NECROSIS AT 7.5 MG/KG EVERY OTHER DAY FOR 5 DAYS. LETHALITY OCCURRED
AT DOSAGES AS LOW AS 2 MG/KG/DAY FOR 12 DAYS. THE DATA APPEAR TO
INDICATE THAT SALMON MAY BE A POTENTIAL MODEL FOR STUDYING THE
NEPHROTOXIC POTENTIAL OF CHEMICALS.
PAGE 33
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SCHOOR, W. PETER. IN PRESS, EXPOSURE OF FISHES TO B£NZOCA)PYR£NE AND SOME
ASPECTS OF ANALYSIS OF METABOLITES. IN: PROCEEDINGS OF THE SYMPOSIUM ON THE
USE OF SMALL FISH SPECIES IN CARCINOGENICITY TESTING, 8ETHESOA, MARYLAND,
DECEMBER 8-10, 1981. U.S. NATIONAL CANCER INSTITUTE. CERL,GB 45*).
THE DISPOSTION OF 6ENZOCA>PYRENE CBCA)PJ IN MOST ANIMAL SPECIES
DEPENDS LARGLEY ON THE EFFECT THAT THE MIXED FUNCTION OXYGENASE
-------
SCHOOR, W. PETER, AND MEERA SRIVASTAVA. 1983. EFFECTS OF TRICHLOROPROPENE
OXIOg ON THE METABOLISM OF 3ENZO(ft)PYRENE 8Y 3-METHVLCHOLANTHREME- AND
PHENOBARBITAL-TREATEO MULLET (MUGIL CEPHALUS), A MARINE FISH CA8STRACT). IN:
PROGRAM AND ABSTRACTS OF FIRST INTERNATIONAL SYMPOSIUM ON FOREIGN COMPOUND
METABOLISM, OCT. 30 THRU NOVEMBER 4, 1983, WEST PALM BEACH, FLORIDA. (ERL,GB
490).
MARINE FISH, MULLET (MUGIL CSPHALUS), MERE TREATED WITH
3-METHVLCHOLANTHRENE (3-MC) AND PHENOBAR8ITAL (PB) BY INTRAPERITONEAL
INJECTION, ANO THE EFFECTS OF TRICHLORQPROPENE OXIDE CTCPO), AN
INHIBITOR OF EPOXIOE HYDRATASE ACTIVITY, ON THE METABOLISM OF
BENZO(A)PYRENE (BAP) BY ISOLATED LIVER MICROSOMES WERE EVALUATED. THE
MOST SINGIFICANT EFFECT FOUND WAS AN INCREASE IN THE OXIDATION OF BAP
AT THE BAY REGION (POSITION 9 AND 10) IN FISH WHICH WERE PRETREATEO
WITH 3-MC ANO PB. NO SIGNIFICANT DIFFERENCES WERE FOUND IN THE
METABOLIC PATTERNS OF 3-MC- AND PS-TREATED FISH. WHEN METABOLITE
PATTERNS OF THE OIHYOROOIOLS IN THE PRESENCE AND ABSENCE OF TCPO WERE
COMPARED, THE PREDICTED SHIFTS WERE OBSERVED IN CONTROL AS WELL AS
3-MC- AND PB-TREATED ANIMALS. COMPARISON OF METABOLITE PATTERNS IN THE
PRESENCE OF TCPO SHOWED DISTINCT DIFFERENCES BETWEEN CONTROL ANO 3-MC-
AND PB-TREATEO ANIMALS. NO SIGNIFICANT DIFFERENCE IN METABOLITE
PATTERNS WERE FOUND BETWEEN 3-MC- ANO PB-TREATEO ANIMALS IN EITHER
PRESENCE OR ABSENCE OF TCPO. COMPARISON TO MAMMALIAN DATA SHOWED THE
INDUCTION-RELATED METABOLITE PATTERN IN FISH TO BE VERY SIMILAR TO
THAT FOUND IN RATS.
SCHOOR* W« PETER* ANO MEERA SRIVASTAVA. 1983. INDUCTION OF MFQ IN MULLET
LIVER MICROSOMESi EFFECT OF NADPH ON BENZO(A)PYRENE METABOLITE DISTRIBUTION
AT 25 DEGREES AND 37 DEGREES (ABSTRACT). PRESENTED AT THE SECOND
INTERNATIONAL SYMPOSIUM ON POLLUTANT RESPONSE IN MARINE ORGANISMS, APRIL
27-29, 1983, WOODS HOLE, MA. (ERL,GB 166).
MIXED-FUNCTION OXYGENASE ACTIVITIES IN MULLET (MUGIL CEPHALUS) WERE
INDUCED WITH 3-METHYLCHOLANTHRENE AND PHENQBARBITAL (PB) ADMINISTERED
INTRAPERITONEALLY TWICE OVER A THIRTY-DAY PERIOD. CONTROL ANO INDUCED
MFO ACTIVITIES WERE STUDIED AT 25 DEGREES AND 3? DEGREES CELSIUS USING
0*1 - 4.0 MM NADPH. INCREASES IN NADPH CONCENTRATIONS IN ACTIVITY
DETERMINATIONS OF CONTROL MULLET MFO AT BOTH TEMPERATURES SHOWED
CORRESPONDING ACTIVITY INCREASES AND RATHER BROAD ACTIVITY OPTIMA. NO
SIGNIFICANT CHANGES IN THE METABOLITE COMPOSITION WERE OBSERVED. MFO
ACTIVITIES INDUCED BY 3-MC ANO CONDUCTED AT 37 DEGREES CELSIUS SHOWED
INCREASES OF ALL METABOLITES EXCEPT THE 4,5-EPOXlOE ANO 5-PHENOL WITH
INCREASING NADPH CONCENTRATIONS. NO SUCH INCREASES WERE FOUND AT 25
DEGREES CELSIUS. INCREASES IN THE INDIVIDUAL METABOLITES WERE
DISPROPORTIONATELY HIGHER AT 37 DEGREES CELSIUS ANO HIGH NADPH
CONCENTRATIONS WHEN COMPARED AT 25 DEGREES CELSIUS. SIMILAR TRENDS
WERE FOUND WITH THE PB INDUCED MFO ACTIVITIES. THE DATA INDICATE THAT
WITH REGARD TO COMPARATIVE INDUCTION. PHYSIOLOGICAL TEMPERATURE
RANGES MAY MAKE COMPARISON OF ACTIVITIES DIFFICULT BETWEEN WARM- AND
COLO-BLOOOED SPECIES. PRELIMINARY DATA AT LOWER TEMPERATURES INDICATE
THAT THE ANOMALIES OF MFO ACTIVITY IN THE MULLET OCCUR AT 37 DEGREES C
RATHER THAN AT THE LOWER TEMPERATURES.
PAGE 35
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SCHOORt W.P. 1979. FLUORIMETRIC CONFIRMATION OF METABOLITES OF
8ENZO(A)PYRENE FROM A MARINE FISH (ABSTRACT). IN: FOURTH INTERNATIONAL
SYMPOSIUM ON POLYNUCLEAR AROMATIC HYDROCARBONS, COLUMBUS, OHIO, OCT. 2-4,
1979, BATELLE INSTITUTE. PP. 36. (ERL.GB X206).
SPECIMEN OF MULLET
-------
STRENGTH, D.R., O.V. SARADAM8AL, SHOO-LIH WANG, AND H.H. OARON. 1982.
GLUCURONSYL- AND SULFQ- TRANSFERASES IN FISH EXPOSED TO ENVIRONMENTAL
CARCINOGEN. FED. PROC. 41J1147. (ERL.GB X262>.
A HIGH INCIDENCE OF ORAL LESION WAS OBSERVED IN FERAL FISH TAKEN FROM
HOLDING PONDS AT THE TUSKEGEE, AL SEWAGE TREATMENT PLANT, CJ.M.
GRIZZLE, ET AL., J. FISH DISEASE 4, 345, 1981). UDP-GLUCURQNOSYL
TRANSFERASE AND SULFQ7RANSFERASE WERE FOUND TO BE ELEVATED IN LIVERS
OF CHANNEL CATFISH AND BLACK BULLHEADS EXPOSED TO EFFLUENT IN SEVERAL
POND LOCATIONS. THE INDUCTION OF THE TRANSFERASES IN EXPOSED FISH,
COMPARED TO CONTROL, WAS REMARKABLY SIMILAR TO INDUCTION OF THE TWO
ENZYMES IN RATS AND FISH GIVEN ORAL OR INTRAPERITONEAL DOSAGES OF
KNOWN CARCINOGENS, 3-METHYLCHOLANTHRENE, 3£NZO(A)PYRENE CBP) OR THE
COMPOUNDS PHENANTHRENE AND PHEN08ARBITAL. FISH MICROSOMES FROM INDUCED
ANIMALS OXIDIZED 3P IN A MIXED FUNCTION OXIOASE SYSTEM. THE PRODUCTS
WERE EXTRACTED FROM THE REACTION MIXTURE INTO ETHVLACETATE FOR
ANALYSIS BY HPLC WITH BOTH UV AND FLUORESCENCE DETECTORS. A NUMBER OF
PHENOLS WERE IDENTIFIED. CONJUGATION OF THE OZIOATION PRODUCTS OF SP
WITH BOTH SULFATE AND GLUCURONIC ACID WAS OBSERVED. ADDITION OF EITHER
UDPGA OR PAPS WITH SULFOTRANSFERASE CONJUGATED THE OXIDATION PRODUCTS
WITH GLUCURONIC ACID OR SULFATE, RENDERING THEM MORE POLAR AND CAUSING
THEIR DISAPPEARANCE FROM ETHYLACETATE EXTRACTS. CONJUGATION WAS
ESSENTIALLY COMPLETE IN EITHER SYSTEM, INDICATING THAT THE TWO SYSTEMS
ARE COMPLEMENTARY.
SULLIVAN, TIMOTHY J., AND MICHAEL C. MIX. 1983. PYROLYTIC DEPOSITION OF
POLYNUCLEAR AROMATIC HYDROCARBONS DUE TO SLASH BURNING ON CLEAR-CUT SITES.
BULL. ENVIRON. CONTAM. TOXICOL. 31(2):208-215, :i27-128.
-------
TAN, 3ARRIE, MELVIN V. KILGORE, DftVlO L. ELAM, PAUL MELIUS, AND W.P. SCHOOR.
1981. METABOLITES OF BENZO(A)PYRENE IN AROCLOR 1254 TREATED MULLET. IN:
AQUATIC TOXICOLOGY AND HAZARD ASSESSMENT, ASTM STP 737. O.R. BRANSON AND
K.L. DICKSON, EDITORS* AMERICAN SOCIETY FOR TESTING AND MATERIALS*
PHILADELPHIA, PA. PP. 239-246. CERL.G8 X267).
THIS PAPER PRESENTS A SYSTEMATIC APPROACH TO THE SEPERATION AND
CHARACTERIZATION OF BCA)P METABOLITES USING THE EFFICIENT ANALYTICAL
TECHNIQUE OF HIGH PRESSURE LIQUID CHRQMATOGRAPHY CHPLO. HEPATIC S-9
PREPERATIONS FROH ARCLOR 1254 TREATED MULLET CMUGIL CEPHALUS) MERE
USED TO STUDY THE INVITRO METABOLISM OF 8(A)P, THE LIPID-SOLUBLE
HVDROXYLATED PRODUCTS WERE EXTRACTED IN ETHYL ACETATE PRIOR TO HPLC
ANALYSIS. THREE DIOLS <9,10; 7,8; AND 4*5) AND TWO PHENOLS <9 AND 3)
OF BCA)P WERE ISOLATED AND IDENTIFIED. FROM THEIR UNRESOLVABLE
QUINONES. THE RESULTS OBTAINED IN THE MULLET WERE COMPARED TO THOSE
OBTAINED IN THE RAT (RATTUS NORVEGICUS).
TAN, 8ARRIE, AND PAUL MELIUS. 1981. INDIRECT ATOMIC ABSORPTION
SPECTROMETRIC ASSAY FOR EPOXIDE HYDROLASE. ANAL. LETT. 14(85):311-322.
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TAN, BARRIE, AND PAUL MELIUS. 1982. SENZOCft3PYRENE METABOLISM IN HEPATIC
S-9 FRACTIONS OF AROCLOR 1254-TREATED MULLET CMUGIL CEPHALUS). IN:
POLYNUCLEAR AROMATIC HYDROCARBONS: PHYSICAL AND BIOLOGICAL CHEMISTRY. M.
COOKE ET AL., EDITOR, 8ATTELLE PRESS, COLUMBUS, OH. PP. 801-811. (ERL,G8
X354).
BIOTRAMSFORMATION OF XENOBIOTIC CHEMICALS IN AQUATIC SPECIES IS LESS
WELL KNOWN THAN THAT IN MAMMALIAN SPECIES. SOME EXAMPLE OF
8ENZOCA3PYRENE (BAP) METABOLITES WHICH HAVE BEEN DETECTED USING TISSUE
PREPARATIONS FROM MARINE FISH INCLUDE 3-HYDROXY8ENZOCA1PYRENE
<3-OH-8AP) €1,2), 8AP-3.6-QUINONE (2), AND
7,8-DIMYOROXY-DIHYDROBENZQCA3PYR£NE CBAP-7,8-DIOL) <3). RECENTLY,
BAP-4,S-OIOL, BAP-7,8-DIOL, BAP-9,10-OIOL, 3-QH-BAP, 9-OH-BAP, AND
QUINONES WERE IDENTIFIED IN TROUT (4,5), SCUP (6), AND SKATE <7) WHEN
LIVER MICROSOMES WERE INCU8ATEO WITH BAP. VARANASI ET AL. <8> HAVE
IDENTIFIED THE BAP METABOLITES IN FLOUNDER, SOLE AND SALMON WHILE WE
HAVE SEPARATED THESE METABOLITES IN MULLET, SEA CATFISH AND GULF
KILLIFISH C9»10). IN ORDER TO ASCERTAIN THE 8IQTRANSFORMATION PATHWAYS
OF XENOBIOTICS* IT IS NECESSARY TO OBTAIN PROFILES OF METABOLITES AND
THE FACTORS THAT INFLUENCE THEIR FORMATION. THIS PAPER PRESENTS THE
PRODUCT PATTERNS OF PHENOLS, OIOLS, AND QUINONES PRODUCED BY THE
AROCLOR-TREATEO MULLET (MUGIL CEPHALUS). EFFECTS OF INCUBATION TIME,
TEMPERATURE* PROTEIN CONCENTRATION AND ENZYME INDUCTION ON THE BAP
METABOLISM WERE INVESTIGATED.
TAN, BARRIE, PAUL MELIUS, AND JOHN GRIZZLE. 1981. HEPATIC ENZYMES AND TUMOR
HISTOPATHOLOGY OF SLACK BULLHEADS WITH PAPILLOMAS. IN: CHEMICAL ANALYSIS
AND BIOLOGICAL FATE: POLYNUCLEAR AROMATIC HYDROCARBONS. MARCUS COOKE AND
ANTHONY J. DENNIS, EDITORS, 3ATTELLE PRESS, COLUMBUS, OH. PP. 377-386.
(6RL.GB X276).
IN THIS STUDY, AN UNUSUALLY HIGH INCIDENCE OF PAPILLONAS C70+-5X) IN
BLACK BULLHEADS CICTALURUS MELAS) FOUND IN A TWO-ACRE FINAL OXIDATION
SEWAGE POND. THE UNIQUE FEATURE WAS THAT THE AERATION (MAINTAINED BY 4
LARGE AERATORS) AND CHLORINATION (3MG CHLORINE/LITER) PROCESSES WERE
ACHIEVED IN THIS FINAL OXIDATION PONO. THIS REPORT DESCRIBES A
BIOCHEMICAL (HEPATIC ENZYMES) COMPARISON OF THE BULLHEADS IN TWO PONDS
(POLLUTED SEWAGE TREATMENT POND AND POLLUTION-FREE NEARBY POND),
•LABORATORY** EXPERIMENTS THAT EMPLOYED SOME SILVER CARP
(HYPOPHTHALMICTHYS MOLITRIX) INTRODUCED INTO THE SEWAGE POND, A BRIEF
SUMMARY OF THE TUMOR HISTQPATHOLOGY, AND A DISCUSSION OF THE POSSIBLE
ROUTE/AGENT(S) RESPONSIBLE FOR THE HIGH TUMOR INCIDENCE.
TAN, BARRIE* PAUL MELIUS, AND KELVIN V. KILGORE. 1980. DETERMINATION OF
1,2-OIOLS BY INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAD PERIOOATE. ANAL.
CHEM, 52C3)«602-604. (ERL.GB X144).
TECHNIQUES ARE DESCRIBED FOR THE DETERMINATION OF 1,2,-DIOLS BY
INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAD PERIODATE. PRELIMINARY
EXPERIMENTS INDICATE THAT STYRENE GLYCQL CAN BE DETERMINED IN THE
PRESENCE OF STYRENE EPOXIOE. THIS IS OF PARTICULAR INTEREST BECAUSE
EPOXIOE HYDRASE CATALYZES THE HYDROLYSIS OF AN EPOXIOE TO A 1,2-DIOL.
THE PROCEDURE MAY LEND ITSELF TO THE DETERMINATION OF THE ENZYME
ACTIVITY OF EPOXIOE HYDRASE, KNOWN FOR ITS PIVOTAL ROLE IN THE
METABOLISM OF MANY CARCINOGENS AND TOXIC COMPOUNDS.
PAGE 39
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TAN, BARRIE, PAUL MELIUS, AND PAUL ZIEGLER. 1932. SIMPLE GAS
CHROMATOGRAPHIC METHOD FOR THE STUDY OF ORGANIC SOLVENTS: MOISTURE ANALYSIS,
HYGROSCOPICITY, AND EVAPORATION, j. CHROMATOGR. sci. 20C5):zu-2i7.
(ERL,G8 X278).
THIS PAPER PRESENTS TH£ BASELINE SEPARATION OF EIGHT COMMON LABORATORY
ORGANIC SOLVENTS BY GC WITH TCO AND SIMPLE ISOTHERMAL CONDITIONS. THE
INFLUENCE OF COLUMN TEMPERATURE ON THE CHROMATOGRAPHIC PARAMETERS,
CAPACITY FACTOR CO, AND RESOLUTION CR>, WAS STUDIED. RAPID ANALYSIS
OF MOISTURE IN ORGANIC SOLVENTS WAS DEMONSTRATED. A LINEAR DETECTION
RESPONSE OF 100 PPM TO 30«
-------
KEYWORD TITLE INDEX
41
-------
KEYWORD TITLE INDEX P I
A T
G E
g M
A
ABILITY OF AH8YSTOMA TIGRINUM HEPATIC MICROSOMES FROM POLYCYLIC AROMAT 1- 2
ABSORPTION WITH DIGESTED LcAO PERIODATE /.DETERMINATION OF 1,2-DlOLS B 39- 3
" SPECTROMETRIC ASSAY FOR EPOXIOE HYDROLASE /.INDIRECT ATOMIC 38- 2
ACCUNULATIflN OF NAPHTHALENE 8Y THE OYSTER OSTREA EDUtlS, IN A FLOW THR 32- 1
ACID TAUftlNE /,THIN-LAYER SEPARATION OF CITRIC ACID CYCLE INTERMEDIATE 32- 2
• CYCLE INTERMEDIATES, LACTIC ACIO, AND THE AMINO ACIO TAURIN6 /,TH 32- 2
" AND THE AMINO ACIO TAURINE /.THIN-LAYER SEPARATION OF CITRIC ACI 32- 2
ACTSVAYXW/•DETERMINATION OF VANADIUM IN A MARINE MOLLUSC USING A CHE 17- 1
AOWCTOR MUSCLE PROTEINS FROM 8IVALVE MOLLUSCS /,EFFECTS OF SODIUM CHL 36- 2
ADVANTAGES AMD SPECIAL PROBLEMS /.USEFULNESS OF CYPRINODON VARIE6ATUS 10- 1
APtATOXIII ON THg BROWN BULLHEAD ICTALURUS NE3ULOSIS. M.S. THESIS /,EF 2- 1
ALTERATIONS IN CARCINOGEN-EXPOSED TELEOSTS: PROCEDURES FOR PREPARATIO 21- 1
Attsvsvawr TIGRINUM HEPATIC MICROSOMES FROM POLYCYLIC AROMATIC HYOROCAR i- 2
AMERICA'/.CELLULAR PROLIFERATES DISORDER IN OYSTERS (OSTREA CHILENSIS 25- 2
AMERICA** OYSTER CCRASSOSTREA VIRGINICA) AS AN INDICATOR OF CARCINOGENS 5- 2
" OYSTER CRASSOSTREA VIRGINICA /.8ENZOCA)PYRENE METABOLISM IN T 1-1
" OYSTER CCRASSOSTREA VIRGINICA) /.CONCURRENT NEOPLASTIC AND PR 9- 2
AMINES /.DIFFERENTIAL ABILITY OF AMBYSTOMA TIGRINUM HEPATIC MICROSOMES 1- 2
AMINO ACID TAURINE /.THIN-LAYER SEPARATION OF CITRIC ACID CYCLE INTERN 32- 2
AMMONIA FROM SMALL VOLUMES OF SEAWATER /.ION-EXCHANGE TECHNIQUE FOR CO 33- 1
ANIMAL MODEL FOR EXPERIMENTAL CARCINOGENESIS /.RAINBOW TROUT CSALMO GA 14- 1
* IN STUDIES INVOLVING CARCINOGENESIS* TERATOGENESIS AND MUTAGENE 16- 1
ANIMALS AS INDICATORS OF ENVIRONMENTAL EXPOSURES /.AQUATIC 4- 3
" /.EFFECTS OF CARCINOGENS. MUTAGENS. AND TERATOGENS ON NON-HUM 8- 1
• /.EFFECTS OF CARCINOGENS. MUTAGENS AND TERATOGENS ON NON-HUMAN T- 2
* : FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM /.EFFECT 8- 2
* /.EFFECTS OF CARCINOGENS. MUTAGENS. AND TERATOGENS ON NON-HUM 8- 1
* INDUCED BY CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTAMINANTS. 23- 1
ANOMALIES THAT OCCUR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT CHEMI 30- 2
APPLICATION IN CARCINOGEN EXPOSURE STUDIES /.PRIMARY CELL CULTURES FRO 20- 1
AWATIC ENVIRONMENT /.AMERICAN OYSTER CCRASSOSTREA VIRGINICA) AS AN IN 5- 2
" ANIMALS AS INDICATORS OF ENVIRONMENTAL EXPOSURES 4- 3
* ANIMALS) /.EFFECTS OF CARCINOGENS. MUTAGENS. AND TERATOGENS ON 8- 1
• ANIMALS /.EFFECTS OF CARCINOGENS. MUTAGENS AND TERATOGENS ON N T- 2
* ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM 8- 2
* ANIMALS) /.EFFECTS OF CARCINOGENS. MUTAGENS. AND TERATOGENS ON 8- 1
* ANIMALS INDUCED BY CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTA 23- 1
AROCLOR 1254-TREATEO MULLET CMUGIL CEPHALUS) /.6ENZOCA3PYRENE METABOLI 39- 1
* 12S4 TREATED MULLET /.METABOLITES OF B6NZOCA)PYR£N£ IN 38- 1
ARWUTIC HYDROCARBONS IN BAY MUSSELS CMYTILUS EOULIS) FROM OREGON* USA 28- 2
» HYDROCARBONS IN SOFTSHELL CLAMS FROM COOS BAY, OREGON. USA /, 28- 1
« HYDROCARBONS AND AROMATIC AMINES /.DIFFERENTIAL ABILITY OF AM 1- 2
» AMINES /.DIFFERENTIAL ABILITY OF AMBYSTOMA TIGRINUM HEPATIC M 1-2
" HYDROCARBONS CABSTRACT) /.EXPOSURE OF TWO TELEOST SPECIES TO 20- 2
* HYDROCARBONS CABSTRACT) /.INDUCTION OF GLUCURQNIOE AND SULFA 36- 3
* HYDROCARBONS AND CELLULAR PROLIFERATIVE DISORDERS IN BIVALVE 25- 1
* HYDROCARBONS IN BIVALVE MOLLUSKS IN BAY MUSSELS CMYTILUS EDUL 28- 3
« HYDROCARBONS DUE TO SLASH BURNING ON CLEAR-CUT SITES /.PYROLY 37- 2
* HYDROCARBONS AND METALS IN MOLLUSCS CABSTRACT) /.STUDIES ON P 24- 1
ARSiHU AND OTHER TRACE ELEMENTS IN BAY MUSSSLS CMYTItUS EDULIS) /.SEA IT- 4
Mt?IHt9ff
-------
KEYWORD TITLE INOEX p I
A T
G E
E M
B
BANDING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CELL LIVES / 12- I
SARNACL6, POLLICIPES POLYMERUS FOR MARINE 8IOASSAYS /.PRELIMINARY STUD 26- I
BAY, ORfiftON DURING JUNE 1976 - MAY 1978 /, BENZ«XA)PYRENE CONCENTRATION 27- 3
" MUSSELS, MYTILUS EOULIS /,8ENZOCA)PYRENE CONCENTRATIONS IN SOMATIC 27- 1
" MUSSELS (MYTILUS EOULIS) FROM OREGON, USA /.CONCENTRATIONS OF UNSU 28- 2
" OREGON, USA /.CONCENTRATIONS OF UNSUBSTITUTED POLYCYCLIC AROMATIC 28- 1
" MUSSELS /.EFFECTS OF DEPURATION, SIZE ANO SEX ON TRACE METAL LEVEL 17- 3
« MUSSELS, MYTILUS EDULIS, FROM OREGON: OCCURRENCE, PREVALENCE, SEA 23- 2
11 OREGON! /,OBSERVATIONS ON TH£ ULTRASTRUCTURE OF LARGE CELLS ASSOCI 26- 2
* MUSSELS CMYTILUS EDULIS) FROM OREGON /.POLYNUCLEAR AROMATIC HYOROC 28- 3
« MUSSEL, MYTILUS EOULIS /.SEASONAL VARIATION IN SOFT TISSUE WEIGHTS 17- 2
" MUSSELS CMYTILUS EOULIS) /.SEASONAL VARIATIONS OF ARSENIC ANO OTHE 17- 4
" OREGON. PH.D. THESIS /.TRACE METAL LEVELS IN A POPULATION OF MYT 18- I
BENZIDINE fOXICITY (ABSTRACT) 18- 3
BENIPYRENE CONCENTRATIONS IN MUSSELS CMYTILUS EOULIS) FROM YAQUINA 27- 3
" A)PYREN£ CONCENTRATIONS IN SOMATIC AND GONAD TISSUES OF BAY MUSS 27- 1
" AJPYRENE METABOLISM IN THE AMERICAN OYSTER CRASSOSTREA VIRGINICA 1- 1
« A3PYRENE METABOLISM IN HEPATIC S-9 FRACTIONS OF AROCLOR 1254-TRE 39- 1
" AJPYRENE IN ECONOMICALLY-IMPORTANT BIVALVE MOLLUSKS FROM OREGON 27- 2
" A)PYRENE METABOLITE PATTERNS IN FISH AND RODENTS /.COMPARATIVE 22- 1
" A)PYREN£ BY 3-METHYLCHOLANTHRENE- AND PHENOBARBITAL-TREATEO MULL 35- 1
" A)PYREN6 METABOLISM IN AN OYSTER COSTREA EOULIS)-BACTERIA SYSTEM 2- 2
11 A)PYR6NE AND SOME ASPECTS OF ANALYSIS OF METABOLITES /.EXPOSURE 34- 1
" A)PYRENE FROM A MARINE FISH (ABSTRACT) /.FLUORIMETRIC CONFIRMATI 36- 1
* A)PYRENE METABOLITE DISTRIBUTION AT 25 DEGREES ANO 37 DEGREES ( 35- 2
" A)PYRENE (ABSTRACT) /.LIVER NEOPLASIA ANO INDUCTION OF HEPATIC M 15- 1
" A)PYRiNE IN AROCLOR 1254 TREATED MULLET /.METABOLITES OF 38- 1
* A)PYRENE FROM MULLET (MUGIL CEPHALUS): PROPERTIES AND DETECTION 34- 3
* A)PYRENE BY HIGH-PERFORMANCE LIQUID CHROXATOGRAPHY /.SEPARATION 10- 2
" A)PYRENE METABOLITES (ABSTRACT) /.SIMPLE HIGH PERFORMANCE LIQUI 11- 1
SIOASSAVS /.PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMB 26- 1
BIOCHEMICAL CHARACTERISTICS /.COMPLEX CHRQMATOPHOROMA IN A MARINE TELE 6- 2
8IOMOMITOIIM6 OF COASTAL WATERS—AN OVERVIEW 6- 3
BIVALVE MOLLUSKS FROM OREGON ESTUARIES /.CHEMICAL CARCINOGENS IN 24- 2
" MOLLUSKS FROM OREGON ESTUARIES /.CHEMICAL CARCINOGENS IN THE M 27- 2
* MOLLUSCS /.EFFECTS OF SOOIUM CHLORIDE CONCENTRATION ON ELECTRO 36- 2
• MOLLUSCS FROM OREGON ESTUARIES /.POLYNUCLEAR AROMATIC HYOROCAR 25- 1
• MOLLUSKS IN BAY MUSSELS (MYTILUS EOULIS) FROM OREGON /.POLYNUC 28- 3
BLACK BULLHEADS WITH PAPILLQMAS /.HEPATIC ENZYMES AND TUMOR HISTOPATHO 39- 2
• BULLHEADS, ICTALURUS MELAS (RAFINESQUE). LIVING IN A CHLORINATED 12- 2
BLOOD CELLS OF CYPRINOOON VARIcGATUS (ABSTRACT) /.MORPHOLOGY OF PERIPH 18- 2
BLOOOCO VERTEBRATES /.HEMATOPOIETIC NEOPLASMS IN INVERTEBRATES ANO COL 13- 1
BODY BURDENS AND THE PREVALENCE OF PROLIFERATIVE DISORDERS IN MUSSELS 29- 2
BtOWM BULLHEAD ICTALURUS NEBULOSIS. M.S. THESIS /.EFFECTS OF AFLATOXI 2- 1
BULLHEAD ICTALURUS NEBULOSIS. M.S. THESIS /.EFFECTS OF AFLATOXIN ON T 2-1
BULLHEADS WITH PAPILLQNAS /.HEPATIC ENZYMES ANO TUMOR HISTOPATHOLOGY 0 39- 2
« ICTALURUS MELAS (RAFINESQUE), LIVING IN A CHLORINATED SEWAG 12- 2
BURDENS ANO THE PREVALENCE OF PROLIFERATIVE DISORDERS IN MUSSELS (MYTX 29- 2
« IN THE BAY MUSSEL. MYTILUS EDULIS /.SEASONAL VARIATION IN SOFT 17- 2
BUtNINC ON CLEAR-CUT SITES /.PYROLYTIC DEPOSITION OF POLYNUCLEAR AROMA 37- 2
C
CAt€IMO€tN ASSAY SYSTEM UTILIZING ESTUARINE FISHES /.DEVELOPMENT OF A 19- 2
« /tCLUCURONSYL- AND SULFO- TRANSFERASES IN FISH EXPOSED TO E 37- 1
• ASSAY SUBJECTS /.LABORATORY EVALUATION OF MARINE FISHES AS 5- 1
« EXPOSURE STUDIES /,PRIMARY CELL CULTURES FROM THE TELEOST, 20- 1
* EXPOSED TELEOSTS: PROCEDURES FOR PREPARATION ANO ANALYSIS 21- 1
CAtCINOteitfSZS IN FISH /.CHEMICAL 14- 2
PAGE 43
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KEYWORD TITLE INDEX P I
A T
G E
s M
c
CARCIN06ENESIS /.RAINBOW TROUT (SALMO GAIRDNERI) EMBRYOS: A SENSITIVE 14- 1
" TERATGGENESIS AND MUTAGENESIS /.USEFULNESS OF THE SELF 16- 1
CARCIN06ENICITY TESTING (A FRIENDLY DISCUSSION/DEBATE BY OR. JOHN COUC 6- 1
* TESTING: ADVANTAGES AND SPECIAL PROBLEMS /.USEFULNESS 10- i
CARCINO&EMS IN THE AQUATIC ENVIRONMENT /.AMERICAN OYSTER (CRASSOSTRCA 5- 2
IN BIVALVE MOLLUSKS FROM OREGON ESTUARIES /.CHEMICAL 24- 2
IN THE MARINE ENVIRONMENT. B£NZO<4)PYRENE IN ECONOMICALLY 27- 2
MUTAGENS, AND TERATOGENS ON NON-HUMAN SPECIES (AQUATIC AN 8- 1
MUTAGENS ANO TERATOGENS ON NON-HUMAN SPECIES-AQUATIC ANIM 7- 2
MUTAGENS. AND TERATOGENS ON NONHUMAN SPECIES (AQUATIC ANI 8- Z
MUTAGENSf AND TERATOGENS ON NON-HUMAN SPECIES (AQUATIC AN 8- 1
/•EXPOSURE OF A TELEOST CELL LINE TO SUSPECTED 15- 2
/•SUMMARY OF TISSUE LESIONS IN AQUATIC ANIMALS INDUCED BY 23- 1
CATFISH,^AND GULF KILLIFISH /,MIX£0 FUNCTION OXIDASE IHDUCIBILITY AND 22- 2
CELL LINE FROM A MALE SHSEPSHEAD /.ANALYSIS OF A MARINE FISH 11- 2
" LINES /.C-BANOING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FI 12- 1
" LINE TO SUSPECTED CARCINOGENS /.EXPOSURE OF A TELEOST 15- 2
" CULTURES FROM THE TELECST, CYPRINODON VARIEGATUS: CULTURE ESTABL 20- I
CELLS OF CYPRINOOON VARIEGATUS (ABSTRACT) /.MORPHOLOGY OF PERIPHERAL B 18- 2
" ASSOCIATED WITH PUTATIVE NEOPLASTIC DISORDERS OF MUSSELS, MYTILU 26- 2
CELLULAR PROLIFERATIVE DISORDER IN OYSTERS (OSTREA CHILENSIS) FROM CHI 25- 2
" PROLIFERATIVE DISORDERS IN BIVALVE MOLLUSCS FROM OREGON ESTUA 25- 1
CEPHALUS) /«BEN20CAJPYRENE METABOLISM IN HEPATIC S-9 FRACTIONS OF ARQC 39- 1
" , A MARINE FISH (ABSTRACT) /.EFFECTS OF TRICHLOROPROPENE OXID 35- 1
* : PROPERTIES AND DETECTION (ABSTRACT) /.METABOLITES OF BENZO 34- 3
CERTAIN PATHOLOGICAL CONDITIONS (ABSTRACT) /.ENVIRONMENTAL CONTAMINANT 29- 1
CHARACTERISTICS /,COMPLEX CHROMATOPHOROMA IN A MARINE TELEOST FISH, FU 6- 2
CHELATINC ION EXCHANGE RESIN NEUTRON ACTIVATION /.DETERMINATION OF VAN 17- 1
CHEMICAL CARCXNOGENESIS IN FISH 14- 2
" CARCINOGENS IN BIVALVE MOLLUSKS FROM OREGON ESTUARIES 24- 2
w CARCINOGENS IN THE MARINE ENVIRONMENT. BENZO(A)PYRENE IN ECO 27- 2
• PERTURBATION BY NAPHTHALENE ON GLUCOSE METABOLISM IN THE EURO 33- 2
CHEMICALS? /,CAN TISSUE ANOMALIES THAT OCCUR IN MARINE FISH IMPLICATE 30- 2
CHEMOTHERAPEUTIC AGENTS, ANO POTENTIAL CARCINOGENS /.SUMMARY OF TISSUE 23- 1
CHILE. SOUTH AMERICA /.CELLULAR PROLIFERATES DISORDER IN OYSTERS COST 25- 2
CHILENSIS) FROM CHILOEt CHILE, SOUTH AMERICA /.CELLULAR PROLIFERATIVE 25- 2
CHILOE, CHILE* SOUTH AMERICA /.CELLULAR PROLIFERATIVE DISORDER IN OYST 25- 2
CHLORIDE CONCENTRATION ON ELECTROPHORETIC PATTERNS OF ADDUCTOR MUSCLE 36- 2
CHLORINATED SEWAGE EFFLUENT /.CAUSES OF PAPILLOMAS ON FISH EXPOSED TO 12- 3
" SEWAGE POND /.PAPILLOMAS OF SLACK BULLHEADS, ICTALURUS MEL 12- 2
CHROMATOSRAPttZC METHOD FOR THE STUDY OF ORGANIC SOLVENTS: MOISTURE AN 40- 1
CHROMATOGRAPHY /.SEPARATION OF THE ISOMERIC PHENOLS OF 6€NZO(A)PYRENE 10- 2
» METHOD FOR SEPARATION OF BENZO(A)PYRENE METABOLITES (A 11- 1
CHROMATOPHOROMA IN A MARINE TELEOST FISH. FUNOULUS GRANDIS: MORPHOLOG 6- 2
CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CELL LINES /.C-BANDING 12- 1
CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, ANO THE AMINO ACIO T&URI 32- 2
CLAMS FROM COOS BAY, OREGON, USA /.CONCENTRATIONS OF UNSUBSTITUTEO POL 28- 1
CLIAt-CWT SITES /.PYROLYTIC DEPOSITION OF POLYI4UCLEAR AROMATIC HYOROCA 37- 2
CLYDE O*WE> - DEBATE: THE FISHY SIDE /.MOUSE vs MINNOW: THE FUTURE o 6-1
COASTAL WATERS—AN OVERVIEW /.BIOMONITORING OF 6-3
COMO SAL*G* /,T08RAMYCIN NEPHROTOXICITY AND LETHALITY IN 33- 3
COLLAtMATIVE PROGRAM /.EFFECTS OF CARCINOGENS, MUTAGENS, ANO TERATOGE 8- 2
COMPARATIVE 8ENZO(A)PYRENE METABOLITE PATTERNS IN FISH AND RODENTS 22- 1
COMPLEX-CttROHATOPHOROMA IN A MARINE TELEOST FISH, FUNDULUS GRANDIS: M 6-2
COMPOUNDS ON ESTUARINE FISHES /.EFFECTS OF PETROLEUM 19- 1
CONCENTRATING AMMONIA FROM SMALL VOLUMES OF SEAWATER /.ION-EXCHANGE TE 33- 1
CONCeWTRATIOM ON ELECTRQPHORETIC PATTERNS DF ADDUCTOR MUSCLE PROTEINS 36- 2
COKCdfTRATIONS IN MUSSELS (MYTILUS EDULIS) FROM YAQUINA SAY, OREGON OU 27- 3
PAGE 44
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KEYWORD TITLE IN0EX p I
A T
G E
E M
C
CONCENTRATIONS IN SOMATIC AND GONAD TISSUES OF BAY MUSSELS, MYTILUS 50 27- 1
" OF UNSUBSTITUTED POLYNUCLEAR AROMATIC HYDROCARBONS IN B 28- 2
" OF UNSU3STITUTEO POLYCYCLIC AROMATIC HYDROCARBONS IN SO 28- I
CONCURRENTtIEOPLASTIC AND PROTISTAN DISORDERS IN THE AMERICAN OYSTER C 9-2
CONDITIONS (ABSTRACT) /.ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE 29- I
CONFIRMATION OF METABOLITES OF BSNZOCA)PYREN£ FROM A MARINE FISH (ABST 36- I
CONTAMINANTS AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL CONDITIONS CAB 29- I
" CHEMOTHERAPEUTIC AGENTS. AND POTENTIAL CARCINOGENS /.SUM 23- 1
CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTAMINANTS. CHSMOTHERAPEUTIC A 23- 1
COOS BAY, OREGON, USA /.CONCENTRATIONS OF UNSU3STITUTEO POLYCYCLIC ARO 28- I
CORRELATION OF MIXED-FUNCTION OXIOASE ACTIVITY WITH ULTRASTRUCTURAL CM 34- 2
COTTONSEED OIL TO RAINBOW TROUT (SALMO GAIRDNERI) /.HEPATQCARCINOGENIC 13- 2
COTTONSEEDS AND REFINED COTTONSEED OIL TO RAINBOW TROUT (SALMO GAIRONE 13- 2
COUCH AND OR. CLfOE OAWE) - DEBATE: THE FISHY SIDE /.MOUSE VS MINNOW: 6- 1
CRASSOSTREA VIRGINICA) AS AN INDICATOR OF CARCINOGENS IN THE AQUATIC E 5-2
" VIRGINICA /»8£NZO(A)PYRENE METABOLISM IN THE AMERICAN OYST 1- I
" VIRGINICA) /.CONCURRENT NEOPLASTIC AND PROTISTAN DISORDERS 9- 2
« GIGAS. M.S. THESIS /.EFFECTS OF X- AND GAMMA IRRADIATION 40- 3
» GIGAS) /.REGENERATION OF RADIATION-DAMAGED DIGESTIVE TISSU 40- 2
CRUSTACEA: PENAEIDAE) /.SHRIMPS CARTHROPOOA: 4- i
CULTURE ESTABLISHED AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES /, 20- 1
CULTURES FROM THE TELEOST, CVPRINOOON VARIEGATUS: CULTURE ESTABLISMEN 20- 1
CUT SITES /.PYROLYTIC DEPOSITION OF POLYNUCLEAR AROMATIC HYDROCARBONS 37- 2
CVCLI INTERMEDIATES, LACTIC ACID, AND THE AMINO ACID TAURINE /,THIN-LA 32- 2
CYPRINOOQN VARIEGATUS) (ABSTRACT) /,EM8RYOLOGIC DEVELOPMENT OF THE SHE 30- I
" VARIEGATUS (ABSTRACT) /.HISTOLOGY OF THE POST-PHARYNGEAL DI 31- 1
" VARIEGATUS (ABSTRACT) /.MORPHOLOGY OF PERIPHERAL BLOOD CELL 18- 2
" VARIEGATUS: CULTURE ESTABLISHED AND APPLICATION IN CARCIN 20- i
« VARIEGATUS AND FUNOULUS GRANDIS IN CARCINOGENIC ITY TESTING: 10- 1
CVPRINQOONTIO FISH, RIVULUS MARMORATUS AS AN EXPERIMENTAL ANIMAL IN ST 16- 1
0
DAMAGED DIGESTIVE TISSUES IN JUVENILE PACIFIC OYSTERS (CRASSOSTREA GIG 40- 2
DAWE) - DEBATE: THE FISHY SIDE /.MOUSE vs MINNOWS THE FUTURE OF FISH 6- i
DEBATE: THE FISHY SIDE /.MOUSE vs MINNOW: THE FUTURE OF FISH IN CARC 6- i
» BY OR, JOHN COUCH AND OR. CLYDE DAWE) - DEBATE: THE FISHY SIDE 6- 1
DEGREES AND 17 DEGREES (ABSTRACT) /.INDUCTION OF MFQ IN MULLET LIVER 35- 2
* (ABSTRACT) /.INDUCTION OF MFD IN MULLET LIVER MICROSOMES2 EF 35- 2
DEPOSITION OF POLYNUCLEAR AROMATIC HYDROCARBONS OUE TO SLASH BURNING 0 37- 2
DEPURATION, SIZE AND SEX ON TRACE METAL LEVELS IN BAY MUSSELS /.EFFECT 17- 3
DETECTION (ABSTRACT) /.METABOLITES OF 8ENZO(A)PYRENE FROM MULLET (MUGI 34- 3
DETERMINATION OF 1,2-OIOLS BY INDIRECT ATOMIC ABSORPTION WITH DIGESTED 39- 3
" OF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EX 17- 1
DEVELOPMENT Of A CARCINOGEN ASSAY SYSTEM UTILIZING ESTUARINE FISHES 19- 2
« OF THE SHESPSHEAD MINNOW (CYPRINOOON VARIEGATUS) (ABSTRACT 30- I
DIETARY EXPOSURE TO BEMZOCA)PYRENE (ABSTRACT) /.LIVER NEOPLASIA AND IN 15- 1
DIFFERENTIAL ABILITY OF AMBYSTOMA TIGRINUM HEPATIC MICROSOMES FROM POL 1- 2
DIGESTED LEAO PERIOOATE /.DETERMINATION OF 1,2-DIOLS BY INDIRECT ATOMI 39- 3
DIGESTIVE TRACT OF THE SHEEPSHEAO MINNOW, CYPRINOOON VARIEGATUS (ABSTR 31- 1
« TISSUES IN JUVENILE PACIFIC OYSTERS (CRASSOSTREA GIGAS) /,RE 40- 2
OIOLS SY INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAO PERIOOATE /,DET 39- 3
DISCUSSION/DEBATE BY OR. JOHN COUCH AND OR. CLYDE DAWE) - DEBATE: THE 6- 1
DISEASES OF INVERTEBRATES OTHER THAN INSECTS /.VIRAL 4- 2
DISORDER IN OYSTERS (OSTREA CHILENSIS) FROM CHILOE, CHILE* SOUTH AMERI 25- 2
DISORDERS IN MUSSELS (MYTILUS EOULIS) IN OREGON /,B€NZO(A)PYREM£ BODY 29- 2
« IN THE AMERICAN OYSTER (CRASSOSTREA VIRGINICA) /.CONCURRENT 9- 2
* OF MUSSELS, MYTILUS EDULIS. FROM YAQUINA BAY, OREGON /.OBSER 26- 2
" IN BIVALVE MOLLUSCS FROM OREGON ESTUARIES /,POLYNUCLEAR ARQM 25- 1
DISSERTATION /.EFFECTS OF CHEMICAL PERTURBATION BY NAPHTHALENE ON GLUC 33- 2
PAGE 45
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KEYWORD TITLE INDEX p I
A T
G E
E M
D
DISTRIBUTION AT 25 DEGREES AND 37 DEGREES (ABSTRACT) /.INDUCTION OF M 35- 2
DYSPLASIA IN YOUNG FISH EXPOSED TO THE H6R3ICIOE TRIFLURALIN /.VERTEBR 3- 1
E
EOULIS) IN OREGON /,BENZOP 27- 2
" /.POLYNUCLEAR AROMATIC HYDROCARBONS AND CELLULAR PROLIFERATI 25- 1
ESTUARINE FISHES /.DEVELOPMENT OF A CARCINOGEN ASSAY SYSTEM UTILIZING 19- 2
* FISHES /.EFFECTS OF PETROLEUM COMPOUNDS ON 19- 1
EUROPEAN FLAT OYSTER COSTREA EDULIS): AN IN VIVO KINETIC. PH.D. DISSE 33- 2
* FLAT OYSTER. OSTREA EOULIS /.EFFECTS OF NAPHTHALENE ON GLUCOS 31- 3
EVAPORATION /.SIMPLE GAS CHROMATOGRAPHIC METHOD FOR THE STUDY OF ORGAN 40- 1
EXCHANGE RESIN NEUTRON ACTIVATION /.DETERMINATION OF VANADIUM IN A MAR 17- 1
« TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES OF SEA 33- 1
EXPOSURE OF A TELEOST CELL LINE TO SUSPECTED CARCINOGENS 15- 2
« OF FISHES TO 3ENZO(A)PYRENE AND SOME ASPECTS OF ANALYSIS OF M 34- 1
* OF TWO TELEOST SPECIES TO PQLYCYCLIC AROMATIC HYDROCARBONS
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KEYWORD TITLE INDEX p I
A T
6 £
E M
F
FISH IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /.CAN TISSUE ANOMALIES TH 30- 2
EXPOSED TO CHLORINATED SEWAGE EFFLUENT /.CAUSES OF PAPILLOMAS ON 12- 3
/tCHEMICAL CARCINOGENESIS IN 14- 2
AND RODENTS /.COMPARATIVE BENZOCA)PYRSNE METABOLITE PATTERNS IN 22- 1
FUNOULUS GRANOIS: MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS 6- 2
/.CORRELATION OF MIXED-FUNCTION OXIOASE ACTIVITY WITH ULTRASTRUCT 34- 2
(ABSTRACT) /f£FFECTS OF TRICHLORQPROPENS OXIDE ON THE METABOLISM 35- 1
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KEYWORD TITLE INDEX P I
A T
G E
E M
H
HIGH PERFORMANCE LIQUID CH80MATOGRAPMY METHOD FOR SEPARATION OF 8ENZOC II- I
HISTOLOGY OF THE POST-PHARYNGEAL DIGESTIVE TRACT OF THE SHEEPSHEAD MIN 31- 1
HISTOPATHOL06IC EFFECTS IN THE PITUITARY OF A TELEOST EXPOSED TO THE H 3-2
HISTOPATHOLOGICAL PROGRESSION /,HA£MIC NEOPLASMS OF BAY MUSSELS, MYTIL 23- 2
HISTOPAT*OL06Y OF SLACK BULLHEADS WITH PAPILLOMAS /.HEPATIC ENZYMES AN 39- 2
« OF FOUR SPONTANEOUS NEOPLASMS IN THREE SPECIES OF SALMO 22- 3
HUNAN SPECIES (AJUATIC ANIMALS) /.EFFECTS OF CARCINOGENS, MUTAG6NS. AN 8- 1
" SPECIES-AQUATIC ANIMALS /.EFFECTS OF CARCINOGENS. MUTAGENS AND T 7-2
M SPECIES (AQUATIC ANIMALS) /.EFFECTS OF CARCINOGENS, MUTAGENS. AN 8- 1
HYDROCARBON OXIOASE IN MARINE ORGANISMS (ABSTRACT) /.INDUCTION OF POLY 21- 2
" METABOLISM IN THE MULLET, SEA CATFISH, AND GULF KILLIFISH 22- 2
HVOftOCARSONS IN SAY MUSSELS CMYTILUS EOULIS) FROM OREGON, USA /.CONCEN 28- 2
IN SOFTSHELL CLAMS FROM COOS SAY, OREGON, USA /.C3NCENTRA 28- 1
AND AROMATIC AMINES /.DIFFERENTIAL ABILITY OF AMBYSTOMA T 1-2
CABSTRACT) /.EXPOSURE OF TWO TELEOST SPECIES TO POLYCYCLI 20- 2
(ABSTRACT) /.INDUCTION OF GLUCURONIDE AND SULFATE TRANSFE 36- 3
AND CELLULAR PROLIFERATIVE DISORDERS IN BIVALVE MOLLUSCS 25- 1
IN BIVALVE MOLLUSKS IN SAY MUSSELS (MYTILUS EDULIS) FROM 28- 3
DUE TO SLASH BURNING ON CLEAR-CUT SITES /.PYRQLYTIC DEPOS 37- 2
AND METALS IN MOLLUSCS (ABSTRACT) /.STUDIES ON POLYNUCLEA 24- 1
HVOROtASE /,INDIRECT ATOMIC ABSORPTION SPECTROMETRIC ASSAY FOR EPOXIOE 38- 2
HVGROSCOPICITY, AND EVAPORATION /.SIMPLE GAS CHROMATQGRAPHIC METHOD FO 40- 1
I
ICTALURUS NE8ULOSIS, M.S. THESIS /,EFFECTS OF AFLATQXIN ON THE BROWN 2- 1
" MELAS (RAFINESQUE), LIVING IN A CHLORINATED SEWAGE POND /,PA 12- 2
IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /.CAN TISSUE ANOMALIES THAT OC 30- 2
IMPORTANT BIVALVE MOLLUSKS FROM OREGON ESTUARIES /.CHEMICAL CARCINOGEN 27- 2
INDICATOR OF CARCINOGENS IN THE AQUATIC ENVIRONMENT /.AMERICAN OYSTER 5- 2
INDICATORS OF ENVIRONMENTAL EXPOSURES /.AQUATIC ANIMALS AS 4- 3
INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAD PERIOOATE /,DETERMINATE 39- 3
" ATOMIC ABSORPTION SPECTROMETRIC ASSAY FOR EPOXIOE HYDRQLASE 38- 2
INDUCED BY CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTAMINANTS, CHEMOTH 23- 1
INOUCIBILITY AND POLYAROMATIC HYDROCARBON METABOLISM IN THE MULLET. SE 22- 2
INDUCTION OF GLUCURONIDE AND SULFATE TRANSFERASES BY PHENOBARBITAL AND 36- 3
* OF MFO IN MULLET LIVER MICROSOMES: EFFECT OF NAOPH ON BENZ 35- 2
N OF POLYAROMATIC HYDROCARBON OXIOASE IN MARINE ORGANISMS CABS 21- 2
" OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE RAINBOW TROUT 15- 1
INEXPENSIVE METHOD FOR MEASURING INTEGRATED LIGHT ENERGY /.SIMPLE AND 37- 3
INSECTS /.VIRAL DISEASES OF INVERTEBRATES OTHER THAN 4- 2
INTEGRATED LIGHT ENERGY /.SIMPLE AND INEXPENSIVE METHOD FOR MEASURING 37- 3
INTERMEDIATES, LACTIC ACID, AND THE AMINO ACID TAURINE /.THIN-LAYER SE 32- 2
INVERTEBRATES AND COLD-BLOODED VERTEBRATES /.HEMATOPOIETIC NEOPLASMS I 13- 1
» OTHER THAN INSECTS /.VIRAL DISEASES OF 4- 2
INVOLVING CA8CINOGENESIS, TERATOGENESIS AND MUTAGENESIS /.USEFULNESS 0 16- 1
ION EXCHANGE RESIN NEUTRON ACTIVATION /.DETERMINATION OF VANADIUM IN A 17- 1
M EXCHANGE TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES OF 33- 1
IRRADIATION ON THE JUVENILE PACIFIC OYSTER, CRASSOSTREA GIGAS. M.S. T 40- 3
ISOMERIC PHENOLS OF BENZO(A)PYRENE BY HIGH-PERFORMANCE LIQUID CHROMATO 10- 2
J
JOHN COUCH AND OR. CLYDE DAWE) - DEBATE: THE FISHY SIDE /.MOUSE VS MI 6- 1
JUVENILE PACIFIC OYSTER, CRASSOSTREA GIGAS. M.S. THESIS /.EFFECTS OF 40- 3
" PACIFIC OYSTERS (CRASSOSTREA GIGAS) /.REGENERATION OF RAOIATI 40- 2
K
KILLIFISM /.MIXED FUNCTION OXIDASE INDUCIBILITY AND POLYAROMATIC HYDRO 22- 2
KINETIC. PH.D. DISSERTATION /.EFFECTS OF CHEMICAL PERTURBATION BY NAP 33- 2
L
LABORATORY EVALUATION OF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS 5-1
LACTIC ACID, AND THE AMINO ACID TAURINE /.THIN-LAYER SEPARATION OF CIT 32- 2
PAGE 43
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KEYWORD TITLE INQEX p I
A T
6 E
£ M
L
LARVAL STAGES OF THE GOOSE BARNACLE, POLLICIPES PQLYMERUS FOR MARINE B 26- 1
LAYER SEPARATION OF CITRIC ACIO CYCLE INTERMEDIATES, LACTIC ACID. AND 32- 2
LEAD PERIQOATE /,DETERMINATION OF 1,2-DIOLS BY INDIRECT ATOMIC ABSORPT 39- 3
LESIQMS IN AQUATIC ANIMALS INDUCED BY CONTROLLED EXPOSURES TO ENVIRONM 23- 1
LETHALITY IN COHO SALMON /.TOBRAMYCIN NEPHROTOXICITY AND 33- 3
LEVELS IN BAY MUSSELS /.EFFECTS OF DEPURATION, SIZE AND SEX ON TRACE M 17- 3
« IN A POPULATION OF MYTILUS EDULIS FROM YAQUlNA 3AY, OREGON. PM 18- 1
LIGHT ENERGY /.SIMPLE AND INEXPENSIVE METHOD FOR MEASURING INTEGRATED 37- 3
LIME FROM A MALE SHEEPSHEAD /.ANALYSIS OF A MARINE FISH CELL 11- 2
" TO SUSPECTED CARCINOGENS /.EXPOSURE OF A TELEOST CELL 15- 2
LIMIS /,C-BANDING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CE 12- 1
LIQUID CHROMATOGRAPHY /.SEPARATION OF THE ISOMERIC PHENOLS OF BENZQ(A) 10- 2
" CHROMATOGRAPHY METHOD FOR SEPARATION OF BENZOCA)PYRENE META80LI 11- 1
LIVER OF A MARINE FISH /.CORRELATION OF MIXED-FUNCTION OXIOASE ACTIVIT 34- 2
" MICRQSOMES2 EFFECT OF NADPH ON BENZOCAJPYRENE METABOLITE OISTR 35- 2
" NEOPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASE ENZYME 15- 1
LIVING IN A CHLORINATED SEWAGE POND /.PAPILLOMAS OF BLACK BULLHEADS, I 12- 2
M
MALE SHEEPSHEAD /.ANALYSIS OF A MARINE FISH CELL LINE FROM A 11- 2
MARINE FISH CELL LINE FROM A MALE SHEEPSHEAD /.ANALYSIS OF A 11- 2
FISH CELL LINES /.C-BANDING OF CHROMOSOMES FROM THREE ESTABLISH 12- 1
FISH IMPLICATE SP6CIFIC POLLUTANT CHEMICALS? /.CAN TISSUE ANOMA 30- 2
ENVIRONMENT. B£NZO(A)PYRENE IN ECONOMICALLY-IMPORTANT BIVALVE 27- 2
TELEOST FISH* FUNOULUS GRANOIS: MORPHOLOGICAL AND BIOCHEMICAL 6- 2
FISH /.CORRELATION OF MIXED-FUNCTION OXIDASE ACTIVITY WITH ULTR 34- 2
MOLLUSC USING A CHELATING ION EXCHANGE RESIN NEUTRON ACTIVATION 17- 1
FISH (ABSTRACT) /.EFFECTS OF TRICHLOROPROPENE OXIDE ON THE META 35- I
FISH (ABSTRACT) /.FLUORIMETRIC CONFIRMATION OF METABOLITES OF B 36- 1
ORGANISMS (ABSTRACT) /.INDUCTION OF PQLVAROMATIC HYDROCARBON OX 21- 2
FISHES AS CARCINOGEN ASSAY SUBJECTS /.LABORATORY EVALUATION OF 5- 1
POLLUTION EFFECTS /.PATHOBIOLQGICAL MEASURES OF 15- 3
BIOASSAYS /.PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF US 26- 1
MARMORATUS AS AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING CARCINOGENES 16- 1
MEASURES OF MARINE POLLUTION EFFECTS /.PATHOBIGLOGICAL 15- 3
MEASURING INTEGRATED LIGHT ENERGY /.SIMPLE AND INEXPENSIVE METHOD FOR 37- 3
MELAS (RAFINESQUE). LIVING IN A CHLORINATED SEWAGE POND /.PAPILLOMAS 0 12- 2
METABOLISM IN THE AMERICAN OYSTER CRASSOSTREA VIRGINICA /,BENZO(A)PYRE 1- 1
« IN HEPATIC S-9 FRACTIONS OF AROCLOR 1254-TREATED MULLET (MU 39- 1
" IN THE EUROPEAN FLAT OYSTER (OSTREA EDULIS): AN IN VIVO KIN 33- 2
" IN THE EUROPEAN FLAT OYSTER. OSTREA EOULIS /.EFFECTS OF NAP 31- 3
M OF BENZO(A)PYRENE BY 3-METHYLCHOLANTHRENE- AND PHENOBARBITA 35- 1
« IN AN OYSTER (OSTREA EDULIS)-BACT£RIA SYSTEM. M.S. THESIS 2- 2
» IN THE MULLET, SEA CATFISH, AND GULF KILLIFISH /.MIXED FUNC 22- 2
METABOLITE PATTERNS IN FISH AND RODENTS /.COMPARATIVE BENZO(A)PYRENE 22- 1
• DISTRIBUTION AT 25 DEGREES AND 37 DEGREES (ABSTRACT) /.IND 35- 2
METABOLITES /,EXPOSURE OF FISHES TO 8ENZO(A)PYRENE AND SOME ASPECTS OF 34- 1
" OF §ENZO(A)PYRENE FROM A MARINE FISH (ABSTRACT) /.FLUORIME 36- 1
" OF B£NZO(A)PYRENE IN AROCLOR 1254 TREATED MULLET 38- 1
• OF B£NZO(A)PYRENE FROM MULLET (MUGIL CEPHALUS): PROPERTIE 34- 3
" (ABSTRACT) /.SIMPLE HIGH PERFORMANCE LIQUID CHROMATOGRAPH 11- 1
METAL LEVELS IN BAY MUSSELS /.EFFECTS OF DEPURATION, SIZE AND SEX ON T IT- 3
« BURDENS IN THE BAY MUSSEL, MYTILUS EDULIS /.SEASONAL VARIATION I 17- 2
" LEVELS IN A POPULATION OF MYTILUS EDULIS FROM YAQUINA BAY, OREGO 18- 1
METALS IN MOLLUSCS (ABSTRACT) /.STUDIES ON POLYNUCLEAR AROMATIC HYOROC 24- 1
METHOD FOR MEASURING INTEGRATED LIGHT ENERGY /.SIMPLE AND INEXPENSIVE 37- 3
« FOR THE STUDY OF ORGANIC SOLVENTS: MOISTURE ANALYSIS. HYGROSCO 40- 1
* FOR SEPARATION OF BcNZOCA)PYRENE METABOLITES (ABSTRACT) /.SIMP 11- 1
METHYLCHetAHTHRENE- AND PHENDBARBITAL-TREATED MULLET (MUGIL CEPHALUS)* 35- 1
PAGE 49
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MIXED-FUNCTION OXIOASE
" FUNCTION OXIDASE
" FUNCTION OXIDASE
MODEL FOR EXPERIMENTAL
KEYWORD TITLE INDEX
M
NFO IN MULLET LIVER MICROSOMES: EFFECT OF NADPH ON BENZO(A)PYR£NE ME
MICROSOMES FROM PQLYCYLIC AROMATIC HYDROCARBONS AND AROMATIC AMINES /,
H EFFECT OF NADPH ON BeNZOCA)PYR£N€ METABOLITE OISTRIBUTIO
HINNOW (CVPRINODON VARIEGATUS) (ABSTRACT) /,EM8RYOLOGIC DEVELOPMENT OF
• CYPRINOOON VARIEGATUS (ABSTRACT) /.HISTOLOGY OF THE POST-PHARY
* THE FUTURE OF FISrI IN C ARCINOGENICITY TESTING (A FRIENDLY DIS
ACTIVITY WITH ULTRASTRUCTURAL CHANGES IN THE LI
ENZYMES THE RAINBOW TROUT FOLLOWING DIETARY EXP
INOUCIBILITY ANO POLYAROMATIC HYDROCARBON METAB
CARCINOGENESIS /.RAINBOW TROUT (SALMQ GAIRONERI
MOISTURE ANALYSIS, HYGROSCOPICITY, AND EVAPORATION /,SIMPLE GAS CHROMA
MOLLUSC USING A CHELATING ION EXCHANGE RESIN NEUTRON ACTIVATION /,DET£
MOLLUSCS /.EFFECTS OF SODIUM CHLORIDE CONCENTRATION ON ELECTROPHORETIC
• FROM OREGON ESTUARIES /.POLYNUCLEAR AROMATIC HYDROCARBONS AND
" (ABSTRACT) /.STUDIES ON POLYNUCLEAR AROMATIC HYDROCARBONS AND
MOLLUSKS FROM OREGON ESTUARIES /.CHEMICAL CARCINOGENS IN BIVALVE
« FROM OREGON ESTUARIES /.CHEMICAL CARCINOGENS IN THE MARINE EN
« IN BAY MUSSELS (MYTILUS EOULIS) FROM OREGON /.POLYNUCLEAR ARO
MORPHOLOGICAL ANO BIOCHEMICAL CHARACTERISTICS /.COMPLEX CHROMATOPHOROM
MORPHOLOGY OF PERIPHERAL BLOOD CELLS OF CYPRINODON VARIEGATUS (ABSTRAC
MOUSE vs MINNOW: THE FUTURE OF FISH IN CARCINQGENICITY TESTING (A FRI
NUGIL CEPHALUS) /,BENZOCAJPYRENE METABOLISM IN HEPATIC S-9 FRACTIONS 0
CEPHALUS), A MARINE FISH (ABSTRACT) /,EFFECTS OF TRICHLORQPROPEN
CEPHALUS): PROPERTIES AND DETECTION (ABSTRACT) /.METABOLITES OF
MU LET CMU&IL CEPHALUS) /,8ENZOCA3PYRENE METABOLISM IN HEPATIC S-9 FRA
(MUSIL CEPHALUS), A MARINE FISH (ABSTRACT) /.EFFECTS OF TRICHLO
LIVER MICROSOMES: EFFECT OF NADPH ON BENZO(A)PYRENE METABOLIT
/.METABOLITES OF BENZO(A)PYRENE IN AROCLOR 1254 TREATED
(MUGIL CEPHALUS): PROPERTIES AND DETECTION (ABSTRACT) /,METABO
SEA CATFISH, AMD GULF KILLIFISH /.MIXED FUNCTION OXIDASE INDUC
PROTEINS FROM BIVALVE MOLLUSCS /.EFFECTS OF SODIUM CHLORIDE CON
MYTILUS EDULIS /.SEASONAL VARIATION IN SOFT TISSUE WEIGHTS ANO
MUSSELS (MYTILUS EDULIS) IN OREGON /*B£NZO(A)PYR£NE BODY BURDENS AND T
(MYTILUS EDULIS) FROM YAQUINA BAY, OREGON DURING JUNE 1976 - M
MYTILUS EOULIS /,8ENZQ(A)PYRENE CONCENTRATIONS IN SOMATIC ANO
(MYTILUS EOULIS) FROM OREGON, USA /.CONCENTRATIONS OF UNSUBSTI
/,EFFECTS OF DEPURATION, SIZE AND SEX ON TRACE METAL LEVELS IN
MYTILUS EDULIS, FROM OREGON: OCCURRENCE, PREVALENCE, SEASONA
FROM YAQUINA BAY, OREGON /.OBSERVATIONS ON TH
FROM OREGON /.POLYNUCLEAR AROMATIC HYOROCARBO
/.SEASONAL VARIATIONS OF ARSENIC AND OTHER TR
OF THE SELF-FERTILIZING CYPRINODONTID FISH, R
ON NON-HUMAN SPECIES (AQUATIC ANIMALS) /,EFFE
ON NON-HUMAN SPECIES (AQUATIC ANIMALS) /,SFFE
ON NON-HUMAN SPECIES-AQUATIC ANIMALS /.EFFECTS
MUSCLE
MUSSEL<
MYTILUS EDULIS,
(MYTILUS EDULIS)
(MYTILUS EOULIS)
MUTAGENESIS /.USEFULNESS
MUTAGEHS, ANO TERATOGENS
ANO TERATOGENS
ANO TERATOGENS
AND TERATOGENS ON NONHUMAN SPECIES (AQUATIC ANIMALS): FOURT
ANO TERATOGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS) /,EFFE
MYT LUS EOULIS) IN OREGON /,BENZO(A)PYRENE BODY BURDENS ANO THE PREVAL
EOULIS) FROM YAQUINA BAY* OREGON DURING JUNE 1976 - MAY 1978 /
EOULIS /,BENZO(A)PYRENE CONCENTRATIONS IN SOMATIC ANO GONAD TI
EOULIS) FROM OREGON, USA /,CONCENTRATIONS OF UNSUBSTITUTED POL
EDULIS, FROM OREGON: OCCURRENCE, PREVALENCE, SEASONALITY, ANO
EOULIS, FROM YAQUINA BAY, OREGON /,OBSERVATIONS ON THE ULTRAST
EOULIS) FROM OREGON /.POLYNUCLEAR AROMATIC HYDROCARBONS IN BIV
EDULIS /,SEASONAL VARIATION IN SOFT TISSUE WEIGHTS AND TRACE M
EOULIS) /.SEASONAL VARIATIONS OF ARSENIC AND OTHER TRACE ELEME
EOULIS FROM YAQUINA BAY, OREGON. PH.D. THESIS /,TRACE METAL L
N
NAWM ON BENZO(A)PYRENE METABOLITE DISTRIBUTION AT 25 DEGREES ANO 37
P I
A T
G E
E M
35- 2
1- 2
35- 2
30- I
31- 1
6- 1
34- 2
15- 1
22- 2
14- 1
40- 1
17- 1
36- 2
25- 1
24- 1
24- 2
27- 2
28- 3
6- 2
18- 2
6- 1
39- 1
35- 1
34- 3
39- 1
35- 1
35- 2
38- 1
34- 3
22- 2
36- 2
17- 2
29- 2
27- 3
27- 1
28- 2
17- 3
23- 2
26- 2
28- 3
17- 4
16- 1
8- 1
8- 1
7- 2
8- 2
8- 1
29- 2
27- 3
27- I
28- 2
23- 2
26- 2
28- 3
17- 2
17- 4
18- 1
35- 2
-------
KEYWORD TITLE INDEX p I
A T
G E
E M
N
NAPHTHALENE ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER COSTREA 33- 2
• ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER, OSTREA 31- 3
* ON GLUCOSE TRANSPORT IN THE OYSTER (ABSTRACT) /.STIMULATOR 31- 2
" 8Y THE OYSTER OSTREA EOULIS, IN A FLOW THROUGH SYSTEM /.UP 32- 1
NEBULOSIS. M.S. THESIS /,EFFECTS OF AFLATOXIN ON THE 3ROWN BULLHEAD I 2-1
NEQPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE 15- 1
NEOPLASMS OF BAY MUSSELS, MYTILUS EDULIS, FROM OREGON: OCCURRENCE, PR 23- 2
* IN INVERTEBRATES AND COLO-BLOODED VERTEBRATES /,HEMATOPOIETI 13- 1
" IN THREE SPECIES OF SALMONID FISHES /,HISTOPATHQLOGY OF FOUR 22- 3
NEOPLASTIC AND PROTISTAN DISORDERS IN THE AMERICAN OYSTER CCRASSOSTREA 9- 2
" DISORDERS OF MUSSELS, MYTILUS EOULIS, FROM YAQU1NA BAY, ORE 26- 2
MEPHROTOXICITY AND LETHALITY IN COHO SALMON /.TOBRAMYCIN 33- 3
NEUTRON ACTIVATION /,DETERMINATION OF VANADIUM IN A MARINE MOLLUSC USI 17- 1
NONHUMAN SPECIES (AQUATIC ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLL 8- 2
0
OBSERVATIONS ON THE ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTA 26- 2
OCCUR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /,CAN TIS 30- 2
OCCURRENCE OF CERTAIN PATHOLOGICAL CONDITIONS (ABSTRACT) /,ENVIRONMENT 29- 1
• PREVALENCE, SEASONALITY, AND HISTOPATHOLOGICAL PROGRESSION 23- 2
OIL TO RAINBOW TROUT (SALMO GAIRONERI) /.HEPATOCARCINOGENICITY OF GLAN 13- 2
ORGANIC SOLVENTS: MOISTURE ANALYSIS* HYGROSCOPICITY, AND EVAPORATION to- i
ORGANISMS CABSTRACT) /.INDUCTION OF POLYAROMATIC HYDROCARBON OXIDASE I 21- 2
OSTREA CHILENSIS) FROM CHILOE, CHILE, SOUTH AMERICA /,CELLULAR PROLIFE 25- 2
" EDULIS>S AN IN VIVO KINETIC. PH.D. DISSERTATION /.EFFECTS OF C 33- 2
" EOULIS /*EFFECTS OF NAPHTHALENE ON GLUCOSE METABOLISM IN THE EU 31- 3
" EOULIS>-8ACTE«IA SYSTEM. M.S. THESIS /.EVALUATION OF BENZOCA)P 2- 2
" EOULIS, IN A FLOW THROUGH SYSTEM /.UPTAKE AND ACCUMULATION OF N 32- 1
OVERVIEW /.BIOMONITORING OF COASTAL WATERS—AN 6- 3
OXIOASE ACTIVITY WITH ULTRASTRUCTURAL CHANGES IN THE LIVER OF A MARINE 34- 2
M IN MARINE ORGANISMS (ABSTRACT) /.INDUCTION OF POLYAROMATIC HYO 21- 2
* ENZYMES THE RAINBOW TROUT FOLLOWING DIETARY EXPOSURE TO BENZOC 15- 1
" INDUCI8ILITY AND POLYAROMATIC HYDROCARBON METABOLISM IN THE MU 22- 2
OXIOE ON THE METABOLISM OF 8ENZO(A)PYRcNE BY 3-METHYLCHOLANTHRENE- AND 35- 1
• TREATMENT /.RESPONSES OF THE HEPATIC ENZYMES OF A TELEOST FISH T 38- 3
OYSTER (CRASSOSTREA VIRGINICA) AS AN INDICATOR OF CARCINOGENS IN THE A 5-2
CRASSOSTREA VIRGINICA /,BENZO(A)PYREN5 METABOLISM IN THE AMERIC 1- 1
CCRASSOSTREA VIRGINICA) /.CONCURRENT NEOPLASTIC AND PROTISTAN 0 9-2
-BACTERIA SYSTEM. M.S. THESIS /.EVALUATION OF B 2-2
CABSTRACT) /.STIMULATORY EFFECT OF NAPHTHALENE ON GLUCOSE TRANS 31- 2
OSTREA EOULlSt IN A FLOW THROUGH SYSTEM /.UPTAKE AND ACCUMULATI 32- 1
OYSTERS COSTREA CHILENSIS) FROM CHILOE, CHILE, SOUTH AMERItA /.CELLULA 25- 2
* CCRASSOSTREA GIGAS) /.REGENERATION OF RADIATION-DAMAGED DIGEST 40- 2
p
PACIFIC OYSTER, CRASSOSTREA GIGAS. M.S. THESIS /.EFFECTS OF X- ANO GA 40- 3
" OYSTERS CCRASSOSTREA GIGAS) /.REGENERATION OF RAOIATIQN-OAMAGE 40- 2
PAPILLQMAS ON FISH EXPOSED TO CHLORINATED SEWAGE EFFLUENT /.CAUSES OF 12- 3
" /.HEPATIC ENZYMES ANO TUMOR HISTOPATHOLOGY OF BLACK BULLHEA 39- 2
- OF BLACK BULLHEADS. ICTALURUS MELAS CRAFINESQUE). LIVING IN 12- 2
PATHOBIOLOCICAL MEASURES OF MARINE POLLUTION EFFECTS 15- 3
PATHOLOGICAL CONDITIONS CABSTRACT) /.ENVIRONMENTAL CONTAMINANTS ANO TH 29- 1
PATTERNS IN FISH ANO RODENTS /.COMPARATIVE BENZOCA)PYRENE METABOLITE 22- 1
« OF ADDUCTOR MUSCLE PROTSINS FROM BIVALVE MOLLUSCS /.EFFECTS 0 36- 2
PCNAEIDAE) /.SHRIMPS (ARTHROPODA: CRUSTACEA: 4- 1
PERFORMANCE LIQUID CHROMATOGRAPHY /.SEPARATION OF THE ISOMERIC PHENOLS 10- 2
« LIQUID CHRONATOGRAPHY METHOD FOR SEPARATION OF BENZOCA)PYR 11- 1
PAGE 51
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KEYWORD TITLE INDEX p I
A T
G E
5 M
P
PERIODATE /,DETERMINATION OF 1,2-DIOLS BY INDIRECT ATOMIC ABSORPTION W 39- 3
PERIPHERAL 8LOOO CELLS OF CYPRINODON VARIEGATUS (ABSTRACT) /.MORPHOLOG 18- I
PERTURBATION BY NAPHTHALENE ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT 33- 2
PETROLEUM COMPOUNDS ON ESTUARIME FISHES /,EFFECTS OF 19- 1
PMARYN6EAL DIGESTIVE TRACT OF THE SH€£PSHEAD MINNOW, CYPRINODON VARIEG 31- 1
PMENOBARilTAL-TREATED MULLET CMUGIL CEPHALUS), A MARINE FISH (ABSTRACT 35- 1
" AND POLYCYCLIC AROMATIC HYDROCARBONS (ABSTRACT) /.INOUC 36- 3
PHENOLS OF 8ENZO(A)PYRENE 8Y HIGH-PERFORMANCE LIQUID CMROMATOGRAPHY /, 10- 2
PITUITARY OF A TELEOST EXPOSED TO THE HERBICIDE TRIFLURALIN /.ENLARGSM 3- 2
POLLICIPES POLYMgRUS FOR MARINE 8IOASSAYS /,PRELIMINARY STUDIES TO EVA 26- 1
POLLUTANT CHEMICALS? /tCAN TISSUE ANOMALIES THAT OCCUR IN MARINE FISH 30- 2
POLLUTION EFFECTS /.PATHOBIOLOGICAL MEASURES OF MARINE 15- 3
POLVAROMATIC HYDROCARBON OXIDASE IN MARINE ORGANISMS (ABSTRACT) /,INOU 21- 2
* HYDROCARBON METABOLISM IN THE MULLET, SEA CATFISH, AMD GU 22- 2
POLYCfCilC AROMATIC HYDROCARBONS IN SQFTSHELL CLAMS FROM COOS BAY, ORE 28- 1
" AROMATIC HYDROCARBONS (ABSTRACT) /,EXPOSURE OF TWO TELEOST 20- 2
" AROMATIC HYDROCARBONS (ABSTRACT) /.INDUCTION OF GLUCURONID 36- 3
POLYCYLIC AROMATIC HYDROCARBONS AND AROMATIC AMINES /,DIFFERENTIAL ABI 1- 2
POLYMERUS FOR MARINE BIOASSAYS /.PRELIMINARY STUDIES TO EVALUATE THE P 26- 1
POLYNUCLEAR AROMATIC HYDROCARBONS IN BAY MUSSELS (MYTILUS EOULIS) FROM 28- 2
• AROMATIC HYDROCARBONS ANO CELLULAR PROLIFERATIVE DISORDERS 25- 1
<• AROMATIC HYDROCARBONS IN BIVALVE MOLLUSKS IN BAY MUSSELS ( 28- 3
« AROMATIC HYDROCARBONS DUE TO SLASH BURNING ON CLEAR-CUT SI 37- 2
* AROMATIC HYDROCARBONS AND METALS IN MOLLUSCS (ABSTRACT) /, 24- 1
PONO /tPAPILLOMAS OF BLACK BULLHEADS, ICTALURUS MELAS (RAFINESQUE), LI 12- 2
POPULATION Of MYTILUS EOULIS FROM YAQUINA BAY, OREGON. PH.D. THESIS / 18- 1
POST-PHARVNGEAL DIGESTIVE TRACT OF THE SHEEPSHEAO MINNOW, CYPRINODON V 31- 1
POTENTIAL OF USING EMBRYO ANO LARVAL STAGES OF THE GOOSE BARNACLE, POL 26- 1
" CARCINOGENS /.SUMMARY OF TISSUE LESIONS IN AQUATIC ANIMALS I 23- 1
PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARV 26- 1
PREPARATION ANO ANALYSIS OF SAMPLES FROM SMALL FISH /,SEROLOGIC ALTERA 21- 1
PREVALENCE OF PROLIFERATIVE DISORDERS IN MUSSELS (MYTILUS EDULIS) IN 0 29- 2
* SEASONALITY, AND HISTOPATHQLOGICAL PROGRESSION /.HAEMIC NE 23- 2
PRIMARY CELL CULTURES FROM THE TSLEOST, CYPRINODON VARIEGATUS: CULTUR 20- 1
PROBLEMS /,USEFULNESS OF CYPRINODON VARIEGATUS ANO FUNOULUS GRANOIS IN 10- 1
PROCEDURES FOR PREPARATION AND ANALYSIS OF SAMPLES FROM SMALL FISH /,S 21- 1
PROGRAM /,EFFECTS OF CARCINOGENS* MUTAGENS* AND TERATQGENS ON NONHUMAN 8- 2
PROGRESSION /,HA£MIC NEOPLASMS OF 3AY MUSSELS, MYTILUS EOULIS, FROM OR 23- 2
PROLIFERATIVE DISORDERS IN MUSSELS (MYTILUS EDULIS) IN OREGON /,BENZO( 29- 2
" DISORDER IN OYSTERS (OSTREA CHILENSIS) FROM CHILOE, CHIL 25- 2
« DISORDERS IN BIVALVE MOLLUSCS FROM OREGON ESTUARIES /,PO 25- 1
PROPERTIES AND DETECTION (ABSTRACT) /.METABOLITES OF BENZO(A)PYREN£ FR 34- 3
PROTEINS FROM BIVALVE MOLLUSCS /.EFFECTS OF SODIUM CHLORIDE CONCENTRAT 36- 2
PROTISTAN DISORDERS IN THE AMERICAN OYSTER (CRASSOSTREA VIRGINICA) /,C 9- 2
PUTATIVE NEOPLASTIC DISORDERS OF MUSSELS, MYTILUS EDULIS. FROM YAQUINA 26- 2
PYRENE BODY BURDENS AND THE PREVALENCE OF PROLIFERATIVE DISORDERS IN M 29- 2
CONCENTRATIONS IN MUSSELS (MYTILUS EDULIS) FROM YAQUINA BAY, OR 27- 3
CONCENTRATIONS IN SOMATIC AND GONAO TISSUES OF BAY MUSSELS, MYT 27- 1
METABOLISM IN THE AMERICAN OYSTER CRASSOSTREA VIRGINICA /.BENZO 1- 1
METABOLISM IN HEPATIC S-9 FRACTIONS OF AROCLOR 1254-TREATEO MUL 39- 1
IN ECONOMICALLY-IMPORTANT BIVALVE MOLLUSKS FROM OREGON ESTUARIE 27- 2
METABOLITE PATTERNS IN FISH AND RODENTS /.COMPARATIVE BENZOCA) 22- 1
BY 3-NETHYLCHOLANTHRENE- ANO PHENQBARBITAL-TREATEO MULLET (MUGI 35- 1
METABOLISM IN AN OYSTER (OSTREA EOULIS)-BACTERXA SYSTEM. M.S. 2- 2
ANO SOME ASPECTS OF ANALYSIS OF METABOLITES /.EXPOSURE OF FISHE 34- 1
FROM A MARINE FISH (ABSTRACT) /,FLUORIMETRIC CONFIRMATION OF ME 36- 1
METABOLITE DISTRIBUTION AT 25 DEGREES ANO 37 DEGREES (ABSTRACT 35- 2
(ABSTRACT) /.LIVER NEOPLASIA ANO INDUCTION OF HEPATIC MIXED FUN 15- 1
PAGE 52
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KEYWORD TITLE INOEX p I
A T
c e
£ M
P
PVteNf IN ARQCLOR 1254 TREATED MULLET /.METABOLITES OF BENZO(A) 38- 1
" FROM MULLET (MU6IL CEPHALUS): PROPERTIES AND DETECTION CABSTRA 34- 3
* BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY /.SEPARATION OF THE I 10- I
" METABOLITES (ABSTRACT) /.SIMPLC HIGH PERFORMANCE LIQUID CHROMA 11- 1
PYtOLfTIC SQEPOSITION OF POLYNUCLEAR AROMATIC HYDROCARBONS DUE TO SLASH 37- 2
..*•' ft
RA»IA?I«H*OAMAGEO DIGESTIVE TISSUES IN JUVENILE PACIFIC OYSTERS CCRASS 40- 2
RAFIMESOtff), LIVING IN A CHLORINATED SEWAGE POMO /.PAPIL10HAS OF BLACK 12- 2
MI«Wtl--4fiOUT (SALMO GAIRONERI) /.HEPATOCARCINOGENICITT'UF GLANDLfSS C 13- 2
» TROUT FOLLOWING DIETARY EXPOSURE TO 8£NZQ(A)PYRENE (ABSTRACT) 15- 1
M TROUT CSALMQ GAIRDNERI) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR 14- 1
REPINE* COTTONSEED OIL TO RAINBOW TROUT (SALMO GAIRDNERTD /.HEPATOCARC 13- 2
RE6EVCRATION OF RADIATION-DAMAGED DIGESTIVE TISSUES IN JUVENILE PACIFI 40- 2
RESI* NEUTRON ACTIVATION /9DETERMINATION OF VANADIUM IN A MARINE MOLLU 17- 1
RESPONSES OF TH€ HEPATIC ENZYMES OF A TELEOST FISH TO TRANS-STILBENE 0 38- 3
RIVULUS MARMORATUS AS AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING CARC 16- 1
ROOCNTS /,COMPARATIVE BENZO(A)PYRENE METABOLITE ^PATTERNS IN FISH AND 22- 1
S
SALMO GAIRDNffRI) /tHEPATOCARCINOGENICITY OF GLANOtESS COTTONSEEDS AND 13- 2
• GAIRONERI) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR EXPERIMENTAL c 14- i
SALMON /,?BBRAMYCIN NEPMRQTOXiCITY AND LETHALITY IN COHO 33- 3
SALNOJU0."FISHES /.HISTOPATHOLOGY OF FOUR SPONTANEOUS NEOPLASMS IN THRE 22- 3
SAMPLES ^FKQM SMALL FISH /.SEROLOGIC ALTERATIONS IN CARCINOGEN-EXPOSED 21- 1
SEA CATPfcSM, AND GULF KILLIFISH /.MIXED FUNCTION OXIOASE INDUCIBILITY 22- 2
SEASONAL VARIATION IN SOFT TISSUE WEIGHTS AND TRACE METAL BURDENS IN T 17- 2
« VARIATIONS OF ARSENIC AND OTHER TRACE ELEMENTS IN BAY MUSSELS 17- 4
SEASONALITV. AND HISTOPATHOLOGICAL PROGRESSION /.HAEMIC NEOPLASMS OF B 23- 2
SEAMATIt/,ION-EXCHANGE TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL 33- 1
SELF-FERTILIZING CYPRINOOONTIO FISH, RIVULUS MARMORATUS AS AN EXPERIME 16- 1
SENSITIVf ANIMAL MODEL FOR EXPERIMENTAL CARCINQGENESIS /.RAINBOM TROUT 14- 1
SEPAtATION OF THE ISOMERIC PHENOLS OF BENZOCA)PYREME BY HIGH-PERFORMAN 10- 2
- OF BENZO(A>PYRENE METABOLITES (ABSTRACT) /.SIMPLE HIGH PER 11- 1
• OF CITRIC ACID CYCLE INTERMEDIATES* LACTIC ACID. AND THE AM 32- 2
SEIOtOCIC ALTERATIONS IN CARCINOGEN-EXPOSED TELEOSTS: PROCEDURES FOR 21- 1
SEMACE EFFLUENT /.CAUSES OF PAPILLQMAS ON FISH EXPOSED TO CHLORINATED 12- 3
• PONO /.PAPILLOMAS OF BLACK BULLHEADS. ICTALURUS MELAS (RAFINESQ 12- 2
StX ON TRACE METAL LEVELS IN BAY MUSSELS /.EFFECTS OF DEPURATION* SIZE 17- 3
SMftFSNBAD /i
-------
KEYWORD TITLE INDEX P I
A T
G £
E M
S
SPECIES OF SALMONID FISHES /,HISTOPATHOLOGY OF FOUR SPONTANEOUS NEOPLA 22- 3
SPECIFICiPOLLUTANT CHEMICALS? /,CAN TISSUE ANOMALIES'' TffA'T OCCUR IN MAR 30- 2
SPECTRDMETRIC ASSAY FOR EPOXIOE HYOROLASE /, INDIRECT At'OMI C ABSORPTION 38- 2
SPONTANEOUS NEOPLASMS IN THREE SPECIES Of SALMONID FSSffe /,HISTOPATHO 22- 3
STAGES 0*F-i'THE GOOSE BARNACLE, POLLICIPES POLYMERUS Ft^lMARlNE BIOASSAY 26- 1
STIL8ENE*OXIOE TREATMENT /, RESPONSES OF THE HEPATIC !E'N®YMES OF A TtLEO 38- 3
STIMULATORY EFFECT OF :NAPHTHALENr ON GLUCOSE TRANSPORWIN* TH€ OYSTER ( 31- 2
SULFATE^TRANSFERASES BY PHENOBAR8ITAL AND POLYCYCLIC $R:OMA1TIC HYDROCA 36- 3
SULFO-WTRANSFERASES IN FISH EXPOSED TO ENVIRONMENT AL *%RC>I NOGEN X.GLUC 37- 1
SUSPECTED CARCINOGENS /.EXPOSURE OF A TELEOST CELL LIS^TO* < 15- 2
'
TAURINE /, THIN-LAYER SEPARATION OF CITRIC ACID CYCLE iWTRMEOIATfS^ LA 32- 2
TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES ' Ol^SSJVWATER^/, I 33- 1
TELEOST FISH, FUNDULUS GRANOIS: MORPHOLOGICAL AND BIOCHEMICAL CHA^RACT 6- 2
" EXPOSED TO THE HERBICIDE TRIFLURALIN /, ENLARGEMENT • OF *;AND HIST 3- 2
" CELL LINE TO SUSPECTED CARCINOGENS /, EXPOSURE OF A *, > 15- 2
n SPECIES TO POLYCYCLIC AROMATIC HYDROCARBONS (ABSTRACT) 1 //fEXPQS 20- 2
" CYPRINOOON VARIEGATUS: CULTURE ESTA8LISMENT AND AP. PKMAWON 20- 1
" FISH TO TRANS-STILBENE OXIOE TREATMENT /, RESPONSES : Of 5TB£SIHERA 38- 3
TELEOSTS: PROCEDURES FOR PREPARATION AND ANALYSIS OF SAMPLES FROMPSMA 21- 1
TERATOGENESIS AND MUTAGENESIS /.USEFULNESS OF THE SELF-FER TILIBINGWYP :16- 1
TERATOGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS) /.EFFECTS OF GARjCIJiO '8- 1
w ON NON-HUMAN SPECIES- AQUATIC ANIMALS /» EFFECTS OF CARGI C0NTRiOLIiE0«iE»PQlSO:RES TO E 23- 1
TISSUES OF BAY MUSSELS, MYTILUS tDULIS /,81M'20( A;)P;YRieN6 G.ONSIENTRATIONS 27- 1
" IN JUVENILE PACIFIC OYSTERS (CRASS0SIRSATG$G^S>> ft/ fftEQEN'ER ATION 40- 2
TOBRAHYCIN NEPHROTQXICITY AND LETHALITY IN COHB 'S'AtMON 33-3
TOXICITY (ABSTRACT) /.BEMZIDINE 18- 3
TRACE METAL LEVELS IN BAY MUSSELS /.EFFECTS OF DEPUTATION. SIZ£ AND S6 17- 3
M METAL BURDENS IN THE BAY MUSSEL, MYTILUS EOULIS /.SEASONAL VARIA 17- 2
M ELEMENTS IN BAY MUSSELS (MYTILUS EOULIS) /.ScASONAL VARIATIONS 0 17- 4
" METAL LEVELS IN A POPULATION OF MYTI.LU-S E'DTJLIS FROM VAQUINA BAY, 18- 1
TRACT OF THE SHEEPSHEAO MINNOW* CYPiRINaDON VARl^GJATUS (ABSTRACT) /,HIS 31- 1
TRANS-STILBENE OXIOE TREATMENT /, Rc,SPOWS£S OF ™* HEPATIC ENZYMES QF A 38- 3
TRANSFERASES IN FISH EXPOSED TO ENVIRONMENTAL 'CARCINOGEN /,GLUCURONSYL 37- 1
« 8Y PHENOBAR8ITAL AND POLYCYCLIC AROM:ATIC HYDROCARBONS
-------
KEYWORD TITLE INDEX P I
A T
G E
5 M
T
TROUT (SALMO GAIRDNERI) EM8RYOS: ft SENSITIVE ANIMAL MODEL FOR EXPERIM 14- 1
TUMOR HPSTOPATHOLOGY OF BLACK BULLHEADS WITH PAPILLOMAS /,HEPATIC y€NZY 39- 2
•-,•-• u ., ''
ULTRASTRUCTURAL CHANGES IN THE LIVER OF a MARINE FI'SN /.CORRELATION OF 34- 2
ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTATIVE NEDPLA$Tie*DISO 26- 2
UNSUBSTMUTED POLYNUCLEAR AROMATIC HYDROCARBONS IN BAY MUSSELS (MYTILU 28- 2
" POLYCYCLIC AROMATIC HYDROCARBONS IN SQFTSHELL CLAMS FROM 28- 1
UPTAKE AND ACCUMULATION OF NAPHTHALENE BY TH£ OYSTER"OSTREA EDULIS", IN 32- 1
USA /.CONCENTRATIONS OF UNSU8STITUTEO POLYNUCLEAR AROMATIC HYDROCARBON 28- 2
. " /.CONCENTRATIONS OF iJNSUSSTI TUTEO POLYCYCLIC AROMATIC HYDROCARBONS 28- 1
USEFULNESS OF CYPRINOOON VARIEGATUS AND FUNDULUS GRANDIS IN CARCINOGEN 10- 1
11 OF THE SELF-FERTILIZING CYPRINODONTIO FISH, RIVULUS MARMORA 16- 1
UTILIZING ESTUARINE FISHES /tDEVELOPMENT OF A CARCINOGEN ASSAY SYSTEM 19- 2
v ' ";- •'
VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EXCHANGE RESIN NEUT 17- 1
VARIATION IN SOFT TISSUE WEIGHTS AND TRACE MET&L BURDENS IN THE.BAY MU 17- 2
VARIATIONS OF ARSENIC AND OTHER TRACc ELEMENTS IN BAY MUSSELS CMYTILUS 17- 4
VARIEGATUS) (ABSTRACT) /*EMBRYOL06I.C DcVELOPMENT OF TH£ SH6EPSHEAO MIN 30- 1
" (ABSTRACT) /,HISTOLOGY OF THE POST-PHARYNGEAL DIGESTIVE TRA 31- 1
" (ABSTRACT) /,MORPHOLOGY OF PERIPHERAL BLOOD CELLS OF CYPRIN 18- 2
" CULTURE eSTABLISMENT AND APPLICATION IN CARCINOGEN EXPOSU 20- 1
" AND FUNDULUS GRANDIS IN CARCINOGENICITY TESTING: ADVANTAGE 10- 1
VERTEBRAL DYSPLASIft IN YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN 3- 1
VERTEBRATES /iHEMATOPOIETIC NEOPLASMS IN INVERTEBRATES AND CQLD-BLOOOE 13- 1
VIRAL DISEASES OF INVERTEBRATES OTHER THAN INSECTS V 4- 2
VIRGINICA) AS AN INDICATOR OF CARCINOGENS IN THE AQUATIC ENVIRONMENT'/ 5- 2
n /,BENZO(A>PYRENE METABQLISM IN THE AMERICAN OYSTER CRASSOSTR 1- 1
" /,CONCURRENT NEOPLASTIC AND PROTISTAN DISORDERS IN THE AMER 9- 2
VIVO KINETIC. PH.D. DISSERTATION /,.EFFECTS OF CHEMICAL PERTURBATION 8 33- 2
VOLUMES OF SEAWATER /,ION-EXCHANGE TECHNIQUE FOR CONCENTRATING AMMONIA 33- 1
W
WATERS—AN OVERVIEW /, BIQMONITORING OF COASTAL 6- 3
WEIGHTS AND TRACE METAL BURDENS IN THE BAY MUSSEL, MYTILUS EOULIS /»S£ 17- 2
Y
YAQUINA BAY, OREGON DURING JUNc 1976 - MAY 1978 /,BENZQ(A>PYR£NE CONCE 27- 3
M BAY, OREGON /.OBSERVATIONS ON TH£ ULTRASTRUCTURE OF LARGE CELL 26- 2
" BAY, OREGON. PH.D. THESIS /,TRACE METAL LEVELS IN A POPULATIO 18- 1
YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN /.VERTEBRAL DYSPLASIA 3- 1
PAG5 55
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AUTHOR INDEX
PAGE 56
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AUTHOR INDEX
A
ABBOTT, O.T., JOINT AUTHOR.
PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARVAL
STAGES OF THE GOOSE BARNACLE, PQLLICIPES PQLYMERUS FOR MARINE
SIOASSAYS/,26-1
ABEL, DANIEL C.v JOINT AUTHOR.
USEFULNESS OF THS SELF-FERTILIZING CfPRINOOOMTID FISH, RIVULUS MARMQRATUS AS
AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING CARCINOGENESIS, TERATOGENESIS
AND MUTASENESIS/,16-1
ANDERSON, ROBERT S.
8ENIO(A)PYRENE M£TA80LISM IN THE AMERICAN OYSTSR CRASSOSTREA VIRGINICA/,1-1
DIFFERENTIAL ABILITY Of AMBYSTOMA TIGRINUM HEPATIC MICRQSOMES FROM PQLYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES/,1-2
AULL, J.L., JOINT AUTHOR.
INDUCTION OF GLUCURONIOE AND SULFATE TRANSFERASES 8Y PHEN08ARBITAL AND
POLYCYCLIC AROMATIC HYDROCARBONS
-------
AUTHOR INDEX
C
COUCH, JOHN A.
COMPLEX CHROMATOPHORQMA IN A MARINE TELEOST FISH, FUNOULUS GRANDIS:
MORPHOLOGICAL AND SIOCHEMICAL CHARACTER1STICS/,6-2
CONCURRENT NEOPLASTIC ANO PRQTISTAN OISOROrRS IN THE AMERICAN OYSTER
CCRASSQSTREA VIRGINICA)/,9-2
EFFECTS OF CARCINOGENS, MUTAGENS AND TSRATOGSNS ON NON-HUMAN SPECIES-AQUATIC
ANIMALS/,7-2
EFFECTS DP CARCINOGENSt MUTAG6NS, ANO TERATOGENS ON NON-HUMAN SPECIES
(AQUATIC ANIMALS)/,7-1
EFFECTS OF CARCINOGENS. MUTAGENS, ANO TERATOGENS ON NON-HUMAN SPECIES
-------
AUTHOR INDEX
c
COURTNEY. LEE A., JOINT AUTHOR.
EFFECTS OF CARCINOGENS, MUTAGENS, AND TERATQGENS ON NONHUMAN SPECIES
CAQUATIC ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM/,8-2
LABORATORY EVALUATION OF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS/,5-1
CROSS, FORO A.» JOINT AUTHOR.
8IQMONITORING OF COASTAL WATERS—AN QVSRVIEW/, 6-3
0
OARON, H.H., JOINT AUTHOR.
GLUCURQNSYL- AND SULFQ- TRANSFEREES IN FISH EXPOSED TO ENVIRONMENTAL
CARCINOGEN/,37-1
INDUCTION OF GLUCURONIQE AND SULFATE TRANSFERASES 3Y PHEN08AR8ITAL AND
POLYCYCLIC AROMATIC HYDROCARBONS (ABSTRACT)/,36-3
DAVIS, WILLIAM P.* JOINT AUTHOR.
EFFECTS 3F CARCINOGENS, MUTAGENS, AND TERATOGENS ON NONHUMAN SPECIES
CAQUATIC ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM/,8-2
DAME* CLYDE, JOINT AUTHOR.
HOUSE vs MINNOW: THE FUTURE OF FISH IN CARCINOGENICITY TESTING CA FRIENDLY
OlSCUSSION/OEBATc BY OR. JOHN COUCH AND OR. CLYDE OAWE) - DEBATE: THE FISHY
SIDE/,6-1
OOOSt JESSICA E.t JOINT AUTHOR.
DIFFERENTIAL ABILITY OF AM8YSTQMA TIGRINUM HEPATIC MICROSDMES FROM PQLYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES/,1-2
E
ELAM, 0., JOINT AUTHOR.
INDUCTION OF POLVAROMATIC HYDROCARBON OXIOASS IN MARINE ORGANISMS
(ABSTRACT)/,21-2
ELAM, OAVID L,, JOINT AUTHOR.
METABOLITES OF BENZOCA)PYRENE IN AROCLOR 1254 TREATED MULLET/,38-1
MIXED FUNCTION OXIDASE INDUCIBILITY AND PQLYARQMATIC HYDROCARBON METABOLISM
IN THE MULLET, S£A CATFISH, ANO GULF KILLlFISH/,22-2
ELLCNOER, RUDOLPH 0., JOINT AUTHOR.
ANALYSIS OF A MARINE FISH CELL LINE FROM A MALE SHEEPSHEAO/,11-2
C-BANDING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CELL LINES/,12-1
EXPOSURE OF A TELEOST CELL LINt TO SUSPECTED CARCINOGENS/,15-2
PRIMARY CELL CULTURES FROM THE TELEOST, CYPRINOOQN VARIEGATUS: CULTURE
6STABLISMENT AND APPLICATION IN CARCINOGEN EXPOSURE STUOIES/,20-1
ELNENAETt ELSAYEO A.
SEPARATION OF THE ISOMERIC PHENOLS OF 3ENZO(A)PYRENE BY HIGH-PERFORMANCE
LIQUID CHROMATOGRAPHV/,10-2
SIMPLE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SEPARATION OF
8EN20PYRENE McTABQLITES (ABSTRACT)/,11-1
PAGE 59
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AUTHOR INDEX
E
ELNENAEY, ELSAYEO A., JOINT AUTHOR.
COMPLEX CHROMATOPHOROMA IN A MARINE TELEnST FISH, FUNDULUS GRANOISS
MORPHOLOGICAL ANQ BIOCHEMICAL CHARACTER 1ST 1CS/,6-2
METABOLITES OF BENZOCA)PYREN= FROM MULLET (MUGIL CEPHALUS): PROPERTIES AND
DETECTION (ABSTRACT)/,34-3
F
FOSS, STEVEN S., JOINT AUTHOR.
AMERICAN OYSTER CCRASSOSTREA VIRGINICA) AS AM INDICATOR OF CARCINOGENS IN
THE AQUATIC ENVIRONMENT/,5-2
LABORATORY EVALUATION OF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS/,5-1
G
GOODMAN, LARRY R.* JOINT AUTHOR.
VERTEBRAL DYSPLASIA IN YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN/,3-1
GREENWICH, W. W., JOINT AUTHOR.
aENZIDINE TOXICITY (ABSTRACT)/,18-3
GREENWICH* M.U.t JOINT AUTHOR.
EXPOSURE OF TWO TELEOST SPECIES TO POLYCYCLIC AROMATIC HYDROCARBONS
(ABSTRACT)/,20-2
GREGORY* PAULA E.
ANALYSIS OF A MARINE FISH CELL LINE FROM A MALE SHEEPSHEAD/,11-2
C-BANDING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CELL LINES/,12-1
GRIZZLE* JOHN N.
CAUSES OF PAPILLOMAS ON FISH EXPOSED TO CHLORINATED SEWAGE EFFLUENT/,12-3
PAPILLOHAS OF SLACK 8ULLHEAOS, ICTALURUS MELAS (RAFINESQUE), LIVING IN A
CHLORINATED SEWAGE PONO/,12-2
GRIZZLE* JOHN N.* JOINT AUTHOR.
HEPATIC ENZYMES AND TUMOR HISTOPATHOLOGY OF SLACK BULLHEADS WITH
PAPILLOMAS/,39-2
GUESS* M.M.* JOINT AUTHOR.
PRIMARY CELL CULTURES FROM THE TSLSOST, CYPRINQDON VARIEGATUS: CULTURE
ESTABHSMENT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES/,20-1
H
HANSEN, OAVIO J.* JOINT AUTHOR.
VERTEBRAL OYSPLASIA IN YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN/,3-1
HARSHBARGEi, JOHN C.
HEMATOPOIETIC NEOPLASMS IN INVERTEBRATES AND COLD-BLOODED VERTEBRATES/,13-1
HAMKES* JOYCE W., JOINT AUTHOR.
OBSERVATIONS ON THE ULTRASTRUCTU*E OF LARGE CELLS ASSOCIATED WITH PUTATIVE
NEOPLASTIC DISORDERS OF MUSSELS, MYTILUS EDULIS, FROM YAQUINA BAY,
OREGON/,26-2
NEMINCMAV* SUSAN J., JOINT AUTHOR.
8ENZO
-------
AUTHOR INDEX
H
HEMINGWAY, SUSAN J., JOINT AUTHOR.
POLYNUCLEAR AROMATIC HYDROCARBONS IN BIVALVE MOLLUSKS IN BAY MUSSELS
CMYTILUS EOULIS) FROM OREGON/,28-3
HENORICKS* JERRY D.
CHEMICAL CARCINOGENESIS IN FISH/,14-2
HEPATOCARCINOGENICITY OF GLANDLESS COTTONSEEDS AND REFINED COTTONSEED OIL TO
RAIN80W TROUT (SALMO GAIRDNERI)/,13-2
LIVER NEQPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXtOASE ENZYMES THE
RAINBOW TROUT FOLLOWING DIETARY EXPOSURE TO 3EN20(A)PYRENe CABSTRACT)/,15-1
RAINBOW TROUT
-------
AUTHOR INDEX
K
KING* KEITH I., JOINT AUTHOR.
3EN20
-------
AUTHOR INDEX
M
MARTIN. B. J.
8ENZIOINE TOXICITY CABSTRACT)/,18-3
MARTIN* BILLY J.
DEVELOPMENT OF A CARCINOGEN ASSAY SYSTEM UTILIZING ESTUARINE FISHES/,19-2
EFFECTS OF PETROLEUM COMPOUNDS ON ESTUARINE *=I SHES/, 19-1
EXPOSURE OF TWO TELEOST SPECIES TO POLYCYCLIC AROMATIC HYDROCARBONS
(ABSTRACT)/,20-2
PRIMARY CELL CULTURES FROM TH£ TELEOST, CYPRINQOON VARIEGATUS: CULTURE
ESTA8LISMENT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES/,20-1
MARTIN* BILLY J., JOINT AUTHOR.
ANALYSIS OF A MARINE FISH CELL LINE FROM A MALE SHEEPSHEAD/,11-2
C-8ANOING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CELL LINES/,12-1
EMaRYOLOGIC DEVELOPMENT OF THE SHEEPSHEAO MINNOW
-------
AUTHOR INDEX
M
KELIUS, PAUL* JOINT AUTHOR.
INDIRECT ATOMIC ABSORPTION SPECTSQMETRIC ASSAY FOR EPQXI0E HYDROLASE/,38-2
META60LITES OF 8ENZOC A )PYR£N= IN AROCLOR 1254 TREATED MULLET/,38-1
RESPONSES OF THE HEPATIC ENZYMES OF A TELEOST FISH TO TRANS-STIL8ENE OXIDE
TREATMENT/,38-3
SIMPLE GAS CHRQMATQGRAPHIC METHOD FOR THE STUDY OF ORGANIC SOLVENTS:
MOISTURE ANALYSIS, HYGROSCOPICITY, AND EVAPORATION/,40-1
MEYERS, T.R.
HISTOPATMOLOGY OF FOUR SPONTANEOUS NEOPLASMS IN THREE SPECIES OF SALMONIO
FISHES/,22-3
MEYERS, THEODORE R.
SUMMARY OF TISSUE LESIONS IN A3UATIC ANIMALS INDUCED BY CONTROLLED EXPOSURES
TO ENVIRONMENTAL CONTAMINANTS, CHEMOTHERAPEUTIC AGENTS, ANO POTENTIAL
CARCINOGENS/,23-1
MEYERS, THEODORE R,, JOINT AUTHOR.
LIVER NEOPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE
RAINBOW TROUT FOLLOWING QIETARY EXPOSURE TO 3ENZO(A)PYRENE (ABSTRACT)/,15-1
MIOOLEBROOKS. B.L.» JOINT AUTHOR.
SEROLOGIC ALTERATIONS IN CARCINOGEN-SXPOSEO TELEOSTS: PROCEDURES FOR
PREPARATION AND ANALYSIS OF SAMPLES FROM SMALL FISH/,21-1
MIX* MICHAEL C
STUDIES ON PQLYNUCLEAR AROMATIC HYDROCARBONS AND METALS IN MOLLUSCS
CAaSTRACT)/,24-i
MIX, MICHAEL C.
B£NZO(A)PYRENE BODY 8UROENS ANO THE PREVALENCE OF PROLIFERATIVE DISORDERS IN
MUSSELS CMYTILUS EOULlS) IN OREGON/,29-2
8ENZOCA>PYR£NE CONCENTRATIONS IN MUSSELS CMYTILUS EDULIS) FROM YAQUINA BAY,
OREGON DURING JUNE 1976 - MAY 1978/,27-S
BENZO(A)PYRENE CONCENTRATIONS IN SOMATIC AND GONAO TISSUES OF BAY MUSSELS,
MYTILUS EDULIS/,27-1
CELLULAR PROLIFERATIVE DISORDER IN OYSTERS CQSTREA CHILENSIS) FROM CHILOE,
CHILE, SOUTH AMERICA/,25-2
CHEMICAL CARCINOGENS IN BIVALVE MOLLUSCS FROM OREGON ESTUARIES/,24-2
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT. BENZOCA)PYREN€ IN
ECONOMICALLY-IMPORTANT BIVALVE MDLLUSKS FROM OREGON ESTUARIES/,27-2
CONCENTRATIONS OF UNSUBSUTUTEQ POLYCYCLIC AROMATIC HYDROCARBONS IN
SOFTSHELL CLAMS FROM COOS BAY, OREGON, USA/,28-1
CONCENTRATIONS OF UNSU8STITUTEO POLYNUCLEA* AROMATIC HYDROCARBONS IN BAY
MUSSELS CMYTILUS EOULIS) FROM OREGON, USA/,28-2
ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL
CONDITIONS
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AUTHOR INDEX
M
MIX, MICHAEL C.
HAEMIC NEOPLASMS OF SAY MUSSELS, MYTILUS EDULIS, FROM QREGQNS OCCURRENCE,
PREVALENCE, SEASONALITY, ANO HISTOPATHOLOGIC4L PROGRESSION/,23-2
OBSERVATIONS ON THE ULTRASTRUCTURE OF LASGE CELLS ASSOCIATED WITH PUTATIVE
NEOPLASTIC DISORDERS OF «USS€LS, MYTILUS EOULIS, FROM YAQUIN4 BAY,
OREGON/,26-2
POLYNUCLEAR AROMATIC HYDROCARBONS ANO CELLULAR PROLIFERATIVE DISORDERS IN
8IVALVE MOLLUSCS FROM OREGON ESTUARI5S/,25-l
POLYNUCLEAR AROMATIC HYDROCARBONS IN BIVALVE MOLLUSKS IN 8AY MUSSELS
(MYTILUS EOULIS) FROM OR€GON/,28-3
PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO ANO LARVAL
STAGES OF THE GOOSE 3ARNACLE, PQLLICIPSS POLYMERUS FOR MARINE
6iaA$SAYS/.26-l
MIX, HICMAEL C.t JOINT AUTHOR.
DETERMINATION OF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EXCHANGE
RESIN NEUTRON ACTIVATION/,17-1
EFFECTS OF DEPURATION, SIZE ANO SEX ON TRACE METAL LEVELS IN BAY
MUSSELS/,17-3
EFFECTS OF NAPHTHALENE ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER,
OSTREA EOULIS/,31-3
EFFECTS OF SODIUM CHLORIDE CONCENTRATION ON ELECTROPHORETIC PATTERNS OF
ADDUCTOR MUSCLE PROTEINS FROM 3IVALVE MOLLUSCS/,36-2
ION-EXCHANGE TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES OF
SEAWATER/,33-1
PYROLYTIC DEPOSITION OF POLYNUCLEAR 4ROMATIC HYDROCARBONS DUE TO SLASH
BURNING ON CLEAR-CUT SITES/,37-2
REGENERATION OF RADIATION-DAMAGEO DIGESTIVE TISSUES IN JUVENILE PACIFIC
OYSTERS CCRASSOSTREA GIGAS)/,40-2
SEASONAL VARIATION IN SOFT TISSUS WEIGHTS ANO TRACE METAL BURDENS IN THE BAY
MUSSEL, MYTILUS EOULIS/,17-2
SEASONAL VARIATIONS OF ARSENIC AND OTHER TRACE ELEMENTS IN BAY MUSSELS
(MYTILUS EDULIS)/,17-4
SIMPLE AND INEXPENSIVE METHOD FOR MEASURING INTEGRATED LIGHT ENERGY/,37-3
THIN-LAYER SEPARATION OF CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, AND
THE AMINO ACID TAURINE/,32-2
UPTAKE ANO ACCUMULATION OF NAPHTHALENE BY THE OYSTER OSTREA EDULIS, IN A
FLOW THROUGH SYSTEM/,32-1
MORENO, MARK
EM8RYOLOSIC DEVELOPMENT OF THE SHEEPSHEAO MINNOW (CYPRINOOON VARIEGATUS)
(ABSTRACT)/,30-1
PAGE 65
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AUTHOR INOEX
N
NIXON, J.E.t JOINT AUTHOR.
HEPATOCARCIN06ENICITY OF GLANOLESS COTTONSEEDS AND REFINED COTTONSEED OIL TO
RAIN30W TROUT CSALMO GAIRDNESI)/,13-2
RAINBOW TROUT (SALMQ GAIRQNERI) rMflRYOS: A SENSITIVE ANIMAL MODEL FOR
EXPERIMENTAL CARCINOGENS SIS/,1*-1
P
PATTON, JOHN S.
CAN TISSUE ANOMALIES THAT OCCUR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT
CHEMICALS?/,30-2
PAWLOWSKI, N.E., JOINT AUTHOR.
HEPATOCARCINOGENICITY OF GLANDLESS COTTONSEEDS AND REFINED COTTONSEED OIL TO
RAINBOW TROUT CSALMO GAIRDNERI)/,13-2
PORTER, RONALD C.
HISTOLOGY OF THE POST-PHARYNGEAL DIGESTIVE TRACT OF THE SHEEPSHEAO MINNOW,
CYPRINOOON VARIEGATUS CA8STRACT)/,3l-l
R
RILEY, RONALD T.
EFFECTS OF CHEMICAL PERTURBATION BY NAPHTHALENE ON GLUCOSE METABOLISM IN THE
EUROPEAN FLAT OYSTER COSTREA EDULIS): AN IN VIVO KINETIC. PH.O.
DISSERTATION/,33-2
EFFECTS OF NAPHTHALENE ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER,
OSTREA EDULIS/,31-3
ION-EXCHANGE TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES OF
SEAUATER/,33-1
STIMULATORY EFFECT OF NAPHTHALENE ON GLUCOSE TRANSPORT IN THE OYSTER
CA8STRACT>/,3l-2
THIN-LAYER SEPARATION OF CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACIO, AND
THE AMINO ACID TAURINE/,32-2
UPTAKE AND ACCUMULATION OF NAPHTHALENE BY THE OYSTER OSTREA EDULIS, IN A
FLOW THROUGH SYSTEM/,32-1
RILEY, RONALD T,, JOINT AUTHOR.
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT. 8ENZOCA)PYRENE IN
ECONOMICALLY-IMPORTANT BIVALVE MQLLUSKS FROM OREGON ESTUARIES/,27-2
ROSE, FRANCIS L,t JOINT AUTHOR.
DIFFERENTIAL ABILITY OF AM8YSTOMA TIGRINUM HEPATIC MICRQSOMES FROM POLYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES/,1-2
S
SARAOANSAL, D,V,, JOINT AUTHOR.
GLUCURQNSYL- AND SUL»=0- TRANSFERASES IN FISH EXPOSED TO ENVIRONMENTAL
CARCINOGEN/,37-1
SCANLAN, R.A., JOINT AUTHOR.
RAINBOW TROUT CSALMO GAIRDNERI) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR
EXPERIMENTAL CARCINOGENESIS/,14-1
PAGE 66
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AUTHOR INDEX
S
SCHAEFFE*, RANDY L.t JOINT AUTHOR,
8EN20CA)PYR£NE CONCENTRATIONS IN MUSSELS CMYTILUS EDULIS) FROM YAQUINA BAY,
OREGON DURING JUNE 1976 - MAY 1978/.27-3
BENZOCA)PYR£NE CONCENTRATIONS IN SOMATIC AND GONAO TISSUES OF SAY MUSSELS,
MYTILUS EDULIS/,27-1
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT. BENZOCA)PYRENS IN
ECONOMICALLY-IMPORTANT BIVALVE MQLLUSKS FROM OREGON 5STUARIES/»27-2
CONCENTRATIONS Or UNSU8STITUTEO POLYCYCLIC AROMATIC HYDROCARBONS IN
SOFTSHELL CLAMS FROM COOS BAY, OREGON USA/,28-1
CONCENTRATIONS OF UNSU3STITUTEO POLYNUCL6AR AROMATIC HYDROCARBONS IN BAY
MUSSELS CMYTILUS EQULIS) FROM OREGON, USA/,28-2
ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL
CONDITIONS CA8STRACT)/,29-l
POLVNUCLEAR AROMATIC HYDROCARBONS IN BIVALVE MQLLUSKS IN BAY MUSSELS
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AUTHOR INDEX
s
SCNOOR, W. PETERt JOINT AUTHOR.
EFFECTS OF CARCINOGENS, MUTAGENS, ANO TERATOGENS ON NON-HUMAN SPECIES
(AQUATIC ANIMALS)/,9-1
EFFECTS OF CARCINOGENS, MUTAGENS, ANO TERATOGENS ON NONHUMAN SPECIES
CAQUATIC ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM/,8-2
INDUCTION OF GLUCURONIOE ANO SULFATE TRANSFERASES 3Y PHEN09ARBITAL AND
POLYCYCLIC AROMATIC HYDROCARBONS (ABSTRACT)/,36-3
INDUCTION OF PQLYAROWATIC HYDROCARBON OXIOASS IN MARINE ORGANISMS
(ABSTRACT)/,21-2
METABOLITES OF B£NZO(A)PYR£NE IN AROCLOR 125* TREATED MULLET/,38-1
MIXED FUNCTION OXIQASE INDUCIBILITY ANO POLYAROMATIC HYDROCARBON METABOLISM
IN THE MULLET, ScA CATFISH, ANO GULF KILLIFISH/,22-2
SEPARATION OF THE ISOMERIC PHENOLS OF SENZQCA)PYRENE BY HIGH-PERFORMANCE
LIQUID CHROMATOGRAPHY/,10-2
SIMPLE HIGH PERFORMANCE LIQUID CNRQMATOGRAPHY METHOD FOR SEPARATION OF
d£NZOCA)PY8EN£ METABOLITES (ABSTRACT)/,!1-1
SCHUfiOLfft* T.i.t JOINT AUTHOR.
PAPILLOMAS OF BLACK BULLHEADS, ICTALURUS MELAS (RAFINESQUE), LIVING IN A
CHLORINATED SEWAGE POND/,12-2
SCOTT* A.L.* JOINT AUTHOR.
PAPILLOMAS OF BLACK BULLHEADS, ICTALURUS MSLAS (RAFINESQUE), LIVING IN A
CHLORINATED SEWAGE PQNO/,12-2
SHELTON, DENNIS U.« JOINT AUTHOR.
LIVER NEOPLASIA ANO INDUCTION OF HEPATIC MIXED FUNCTION QXIOASc ENZYMES THE
RAINBOW TROUT FOLLOWING DIETARY EXPOSURE TO 8ENZOCA)PYRENE (ABSTRACT)/,15-1
SINNHIiBCR* R.O-, JOINT AUTHOR.
HEPATOCARCINOGENICITY OF GLANDLESS COTTONSEEDS ANO REFINED COTTONSEED OIL TO
RAINBOW TROUT (SALMO GAIRDNERI)/,13-2
RAINBOW TROUT CSALHO GAIRDNERI) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR
EXPERIMENTAL CARCINQGENESIS/,14-1
SINNHUBER, RUSSELL O.t JOINT AUTHOR.
LIVER NEOPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIOASE ENZYMES THE
RAINBOW TROUT FOLLOWING DIETARY EXPOSURE TO 3ENZOCA)PYRENE (ABSTRACT)/,15-1
SMITH* A.C.
EFFECTS OF SODIUM CHLORIDE CONCENTRATION ON ELECTRQPHORETIC PATTERNS OF
ADDUCTOR MUSCLE PROTEINS FROM BIVALVE MOLLUSCS/,36-2
SMITH* CHARLIE i.* JOINT AUTHOR.
HEMATQPOIETIC NEOPLASMS IN INVSRTE8RATES AND COLD-BLOODED VERTEBRATES/,13-1
SMtKS* ALBERT K.* JOINT AUTHOR.
OBSERVATIONS ON THE ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTATIVE
NEOPLASTIC DISORDERS OF MUSSELS, MYTILUS EOULIS, FROM YAQUINA BAY,
OREGON/,26-2
PAGE 68
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AUTHOR INDEX
S
SRIVASTAVA, MEERA, JOINT AUTHOR.
EFFECTS OF TRICHLQRQPRQPENE OXlOe ON THE METABOLISM OF B*NZOCA)PYREN5 BY
3-METHYLCHOLANTHRENE- AND PHSNQQARBITAL-TREATED MULLET CMUGIL CEPHALUS), A
MARINE FISH CABSTRACT)/,35-1
INDUCTION OF MFO IN MULLET LJVSR MIC40SOMES: cFFECT OF NADPH ON
8ENZO(A)PYRENE METABOLITE DISTRIBUTION AT 25 DEGREES AND 37 DEGREES
(ABSTRACT)/,35-2
STRENGTH, 0.R.
GLUCURONSYL- AND SULFO- TR4NSF5RASES IN FISH EXPOSED TO ENVIRONMENTAL
CARCINOGSN/,37-1
INDUCTION OF GLUCURONIOE AND SULFATE TRANSFERASES 8Y PHENQ8ARBITAL AND
POLYCYCLIC AROMATIC HYDROCARBONS CA9STRACT)/,36-3
SULLIVAN, TIMOTHY J.
PYROLYTIC DEPOSITION OF PQLYNUCLSAR AROMATIC HYDROCARBONS DUE TO SLASH
BURNING ON CLEAR-CUT SITES/,37-2
SIMPLE AND INEXPENSIVE METHOD PQR MEASURING INTEGRATED LIGHT ENERGY/,37-3
T
TAN, BARRIE
6EN20CA3PYRENE METABOLISM IN HEPATIC S-9 FRACTIONS OF AROCLQR 1254-TREATEO
MULLET /»40-2
TRfNHOLH, STEVEN R., JOINT AUTHOR.
BENZOCA)PYRENE BODY BURDENS AND THE PREVALENCE OF PROLIFeRATIVE DISORDERS IN
MUSSELS CMYTILUS EOULIS) IN OREGON/,29-2
PAGs 69
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AUTHOR INDEX
T
TRENHOLM, STEVEN R.» JOINT AUTHOR.
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT. BENZOCA>PYRENE IN
ECONOMICALLY-IMPORTANT BIVALVE M3LLUSKS FROM OREGON ESTUARIES/,27-2
U
WALES* J.H., JOINT AUTHOR.
RAINSOU TROUT
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