EPA-600/3-78-013
January 1978
Ecological Research Series
            DISTRIBUTION OF PHYTOPLANKTON IN
                                      KENTUCKY LAKES
                                Environmental Monitoring and Support Laboratory
                                        Office of Research and Development
                                       U.S. Environmental Protection Agency
                                              Las Vegas, Nevada 89114

-------
                 RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology. Elimination  of  traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:

      1.   Environmental Health Effects Research
      2.   Environmental Protection Technology
      3.   Ecological Research
      4.   Environmental Monitoring
      5.   Socioeconomic Environmental Studies
      6.   Scientific and Technical Assessment Reports (STAR)
      7.   Interagency Energy-Environment Research and Development
      8.   "Special" Reports
      9.   Miscellaneous Reports

This report has been assigned to the ECOLOGICAL RESEARCH series. This series
describes research on the effects of pollution on humans, plant and animal spe-
cies, and materials. Problems are assessed for their long- and short-term influ-
ences. Investigations include formation, transport, and pathway studies to deter-
mine the fate of pollutants and their effects. This work provides the  technical basis
for setting  standards to minimize undesirable changes in living organisms in the
aquatic, terrestrial, and atmospheric environments.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

-------
                                            EPA-600/3-78-013
                                            January 1978
   DISTRIBUTION OF PHYTOPLANKTON IN KENTUCKY LAKES


                      by
W. D. Taylor, F. A. Hiatt*, S.  C. Hern, J.  W.  Hilgert*,
     V. W. Lambou, F. A. Morris*, M. K. Morris*,
                and L. R. Williams

           Monitoring Operations Division
   Environmental Monitoring and Support Laboratory
              Las Vegas, Nevada   89114

         *Department of Biological Sciences
         The University of Nevada, Las Vegas
              Las Vegas, Nevada   89154
   ENVIRONMENTAL MONITORING AND SUPPORT LABORATORY
         OFFICE OF RESEARCH AND DEVELOPMENT
        U.S. ENVIRONMENTAL PROTECTION AGENCY
              LAS VEGAS, NEVADA   89114

-------
                                   DISCLAIMER
     This report has been reviewed by the Environmental  Monitoring and Support
Laboratory-Las Vegas, U.S. Environmental Protection Agency,  and approved for
publication.  Mention of trade names or commercial  products  does not constitute
endorsement or recommendation for use.
                                     ii

-------
                                    FOREWORD


     Protection of the environment requires effective regulatory actions
which are based on sound technical and scientific information.   This infor-
mation must include the quantitative description and linking of pollutant
sources, transport mechanisms, interactions, and resulting effects on man
and his environment.  Because of the complexities involved, assessment of
specific pollutants in the environment requires a total  systems approach
which transcends the media of air, water, and land.   The Environmental
Monitoring and Support Laboratory-Las Vegas contributes  to the  formation
and enhancement of a sound integrated monitoring data base through multi-
disciplinary, multimedia programs designed to:

          •  develop and optimize systems and strategies for moni-
             toring pollutants and their impact on the environment

          •  demonstrate new monitoring systems and technologies
             by applying them to fulfill special monitoring needs
             of the Agency's operating programs

     This report presents the species and abundance of phytoplankton in the
5 lakes sampled by the National Eutrophication Survey in the State of
Kentucky, along with results from the calculation of several commonly used
biological indices of water quality and community structure.  These data
can be used to biologically characterize the study lakes, and as baseline
data for future investigations.  This report was written for use by Federal,
State, and local governmental agencies concerned with water quality analysis,
monitoring, and/or regulation.  Private industry and individuals similarly
involved with the biological aspects of water quality will find the document
useful.  For further information contact the Water and Land Quality Branch,
Monitoring Operations Division.
                                George B. Morgan
                                    Di rector
                 Environmental Monitoring and Support Laboratory
                                    Las Vegas
                                     iii

-------
                                    CONTENTS
                                                              Page
Foreword	iii
Introduction 	     1
Materials and Methods  	     2
     Lakes and Site Selection	     2
     Sample Preparation  	     2
     Examination 	     3
     Quality Control 	     4
Results  	     5
     Nygaard's Trophic State Indices 	     5
     Palmer's Organic Pollution Indices  	     5
     Species Diversity and Abundance Indices 	     7
     Species Occurrence and Abundance  	     8
Literature Cited 	     9
Appendix.  Summary of Phytoplankton Data 	    10

-------
                                INTRODUCTION
     The collection and analysis of phytoplankton data were included in the
National Eutrophication Survey in an effort to determine relationships between
algal characteristics and trophic status of Individual lakes.

     During spring, summer, and fall of 1973, the Survey sampled 250 lakes in
17 States.  Over 700 algal species and varieties were identified and enumerated
from the 743 water samples examined.

     This report presents the species and abundance of phytoplankton in the
5 lakes sampled in the State of Kentucky (Table 1).  The Nygaard's Trophic
State (Nygaard 1949), Palmer's Organic Pollution (.Palmer 1969), and species
diversity and abundance indices are also included.

          TABLE 1.  LAKES SAMPLED IN THE STATE OF KENTUCKY          	
STORET #
LAKE NAME
COUNTY
2101


2102


2103

2104
Lake Cumberland


Dale Hollow Reservoir


Herrington Lake

Kentucky Lake
Pulaski, McCreary, Russell,
Wayne, Clinton

Cumberland, Clinton (Clay,
Pickett, Overton in Tenn.)

Boyle, Mercer, Garrard

Marshall, Lyon, Trigg,
Livingston, Galloway (Henry,
Stewart, Benton, Houston,
Humphreys, Perry, Decatur
tn Tenn.)
2105
Barren River Reservoir
Allen. Barren

-------
                             MATERIALS AND METHODS

LAKE AND SITE SELECTION

     Lakes and reservoirs included in the Survey were selected through
discussions with State water pollution agency personnel  and U.S.  Environmental
Protection Agency Regional Offices (U.S. Environmental Protection Agency
1975).  Screening and selection strongly emphasized lakes with actual  or
potential accelerated eutrophication problems.  As a result, the  selection
was limited to lakes:

     (1) impacted by one or more municipal sewage treatment plant outfalls
         either directly into the lake or by discharge to an inlet tributary
         within approximately 40 kilometers of the lake;

     (2) 40 hectares or larger in size; and

     (3) with a mean hydraulic retention time of at least 30 days.

Specific selection criteria were waived for some lakes of particular State
interest.

     Sampling sites for a lake were selected based on available information
on lake mcrphometry, potential major sources of nutrient input, and on-site
judgment of the field limnologist (U.S. Environmental Protection  Agency
1975),  Primary sampling sites were chosen to reflect the deepest portion
of each major basin in a test lake.  Where many basins were present, selection
was guided by nutrient source information on hand.  At each sampling site,
a depth-integrated phytoplankton sample was taken.  Depth-integrated samples
were uniform mixtures of water from the surface to a depth of 15  feet
(4.6 meters) or from the surface to the lower limit of the photic zone
representing 1 percent of the incident light, whichever was greater.  If
the depth at the sampling site was less than 15 feet (4.6 meters), the
sample was taken from just off the bottom to the surface.  Normally, a lake
was sampled three times in 1 year, providing information on spring, summer,
and fall conditions.
SAMPLE PREPARATION

     Four milliliters (ml) of Acid-Lugol's solution (Prescott 1970) were
added to each 130-ml sample from each site at the time of collection for
preservation.  The samples were shipped to the Environmental Monitoring and
Support Laboratory, Las Vegas, Nevada, where equal volumes from each site
were mixed to form two 130-ml composite samples for a given lake.  One
composite sample was put into storage and the other was used for the
examination.

-------
     Prior to examination, the composite samples were concentrated by the
settling method.  Solids were allowed to settle for at least 24 hours prior
to siphoning off the supernate.  The volume of the removed supernate and the
volume of the remaining concentrate were measured and concentrations determined.
A small (8 ml) library subsample of the concentrate was then taken.  The
remaining concentrate was gently agitated to resuspend the plankton and
poured into a capped, graduated test tube.  If a preliminary examination of a
sample indicated the need for a more concentrated sample, the contents of the
test tube were further concentrated by repeating the settling method.  Final
concentrations varied from 15 to 40 times the original.

     Permanent slides were prepared from concentrated samples after analysis
was complete.  A drop of superconcentrate from the bottom of the test tube
was placed in a ring of clear Karo®Corn Syrup with phenol (a few crystals of
phenol were added to each 100 ml of syrup) on a glass slide, thoroughly
mixed, and topped with a coverglass.  After the syrup at the edges of the
coverglass had hardened, the excess was scraped away and the mount was
sealed with clear fingernail polish.  Permanent diatom slides were prepared
by drying sample material on a coverglass, heating in a muffle furnace at
400  C for 45 minutes, and mounting in Hyrax®.  Finally, the mounts were
sealed with clear fingernail polish.

     Backup samples, library samples, permanent sample slides, and
Hyrax®.mounted diatom slides are being stored and maintained at the
U.S. Environmental Monitoring and Support Laboratory-Las Vegas.


EXAMINATION

     The phytoplankton samples were examined with the aid of binocular
compound microscopes.  A preliminary examination was performed to precisely
identify and list all forms encountered.  The length of this examination
varied depending on the complexity of the sample.  An attempt was made to
find and identify all of the forms present in each sample.  Often forms were
observed which could not be identified to species or to genus.  Abbreviated
descriptions were used to keep a record of these forms (e.g., lunate cell,
blue-green filament, Navicula #1).  Diatom slides were examined using a
standard light microscope.  If greater resolution was essential to accurately
identify the diatoms, a phase-contrast microscope was used.

     After the species list was compiled, phytoplankton were enumerated using
a Neubauer Counting Chamber with a 40X objective lens and a 10X ocular lens.
All forms within each field were counted.  The count was continued until a
minimum of 100 fields had been viewed, or until the dominant form had been
observed a minimum of 100 times.
®Registered Trademark

-------
QUALITY CONTROL

     Internal quality control checks on species identifications and counts
were performed on a regular basis between project phycologists at the rate
of 7 percent.  Although an individual had primary responsibility for analyzing
a sample, taxonomic problems were discussed among the phycologists.

     Additional quality control checks were performed on the Survey samples
by Dr.  G. W. Prescott of the University of Montana at the rate of 5 percent.
Quality control checks were made on 75 percent of these samples to verify
species identifications while checks were made on the remaining 25 percent
of the samples to verify genus counts.  Presently, the agreement between
quality control checks for species identification and genus enumerations is
satisfactory.

-------
                                   RESULTS


     The Appendix summarizes all of the phytoplankton data  collected  from
the State by the Survey.  It is organized by lake,  including  an  alphabetical
phytoplankton species list with concentrations for  individual  species given
by sampling date.  Results from the application of  several  indices  are
presented (Nygaard's Trophic State, Palmer's Organic Pollution,  and species
diversity and abundance).  Each lake has been assigned a four digit STORET
number.  [STORET (STOrage and RETrieval) is the U.S. Environmental  Protection
Agency's computer system which processes and maintains water  quality  data.]
The first two digits of the STORET number identify  the State;  the last two
digits identify the lake.


NYGAARD'S TROPHIC STATE INDICES

     Five indices devised by Nygaard (1949) were proposed under  the assumption
that certain algal groups are indicative of levels  of nutrient enrichment.
These indices were calculated in order to aid in determining  the surveyed
lakes' trophic status.  As a general rule, Cyanophyta, Euglenophyta,  centric
diatoms, and members of the Chlorococcales are found in waters that are
eutrophic (rich in nutrients), while desmids and many pennate diatoms
generally cannot tolerate high nutrient levels and  so are found  in  oligotrophic
waters (poor in nutrients).

     In applying the indices to the Survey data, the number of taxa in each
major group was determined from the species list for each sample.   The
ratios of these groups give numerical values which  can be used as a biological
index of water richness.  The five indices and the  ranges of values established
for Danish lakes by Nygaard for each trophic state  are presented in Table 2.
The appropriate symbol, (E) eutrophic and (0) oligotrophic, follows each
calculated value in the tables in the Appendix.  A  question mark (?)  was
entered in these tables when the calculated value was within the range of
both classifications.


PALMER'S ORGANIC POLLUTION INDICES

     Palmer (1969) analyzed reports from 165 authors and developed  algal
pollution indices for use in rating water samples with high organic pollution.
Two lists of organic pollution-tolerant forms were  prepared, one containing
20 genera, the other, 20 species (Tables 3 and 4).   Each form was assigned
a pollution index number ranging from 1 for moderately tolerant forms to 6
for extremely tolerant forms.  Palmer based the index numbers on occurrence
records and/or where emphasized by the authors as being especially tolerant
of organic pollution.
                                       5

-------
TABLE 2.  NYGAARD'S TROPHIC STATE INDICES ADAPTED FROM HUTCHINSON (1967)
Index
Myxophycean
Chlorophycean
Di atom
Euglenophyte
Compound
Calculation
Myxophyceae
Desmideae
Chlorococcales
Desmideae
Centric Diatoms
Pennate Diatoms
Euglenophyta
Myxophyceae + Chlorococcales
Myxophyceae + Chlorococcales +
Centric Diatoms + Euglenophyta
Desmideae
Oligotrophic
0.0-0.4
0.0-0.7
0.0-0.3
0.0-0.3
0.0-1.0
Eutrophic
0.1-3.0
0.2-9.0
0.0-1.75
0.0-1.0
1.2-25
TABLE 3.  ALGAL GENUS POLLUTION INDEX
          (Palmer 1969)
                                       TABLE 4.  ALGAL SPECIES POLLUTION
                                                 INDEX (Palmer 1969)
Genus
Anaaystis
Ankis trodesmus
Ch lamydomonas
Chlorella
Closteriian
Cyalotella
Euglena
Gomphonema
Lepocinalis
Uelosiva
Mtcvaeti-nium
Navieula
NitssoM-a
Oseillatoria
Pandorina
Fhaeus
Fhormid'i'um
Seenedesmus
Stigeootoniion
Synedva
Pollution
Index
1
2
4
3
1
1
5
1
1
1
1
3
3
5
1
2
1
4
2
2
                                       Species
                                                                    Pollution
                                                                      Index
                                       Ankistpodesmus falaatus
                                           Chlorella vulgapis
                                           Cyolotella menegTvin-iana
                                           Euglena gvaailis
                                           Euglena viridis
                                           Gomphonema parvulim
                                           Melosira varians
                                           Navieula aryptoaephala
                                           Nitssahia aeioulapis
                                           Nitzsahia palea
                                           Osaillatoria ahlorina
                                           Ose-illatovia limosa
                                           Oseillatoria prineeps
                                           Oscillatovia putrida
                                           Qscillatovia tennis
                                           Pandorina morion
                                           Soenedesmus quadiri-eauda
                                           Stigeoolon-ium tenue
                                           Syndva ulna
                                                                    2
                                                                    2
                                                                    2
                                                                    1
                                                                    6
                                                                    1
                                                                    2
                                                                    1
                                                                    1
                                                                    5
                                                                    2
                                                                    4
                                                                    1
                                                                    1
                                                                    4
                                                                    3
                                                                    4
                                                                    3
                                                                    3

-------
     In analyzing a water sample, any of the 20 genera or species of algae
present in concentrations of 50 per milliliter or more are recorded.  The
pollution index numbers of the algae present are totaled, providing a genus
score and a species score.  Palmer determined that a score of 20 or more for
either index can be taken as evidence of high organic pollution, while a
score of 15 to 19 is taken as probable evidence of high organic pollution.
Lower figures suggest that the organic pollution of the sample is not high,
that the sample is not representative, or that some substance or factor
interfering with algal persistence is present and active.


SPECIES DIVERSITY AND ABUNDANCE INDICES

     "Information content" of biological samples is being used commonly by
biologists as a measure of diversity.  Diversity in this connection means the
degree of uncertainty attached to the specific identity of any randomly
selected individual.  The greater the number of taxa and the more equal  their
proportions, the greater the uncertainty, and hence, the diversity (Pielou
1966).  There are several methods of measuring diversity, e.g., the formulas
given by Brillouin (1962) and Shannon and Weaver (1963).  The method which is
appropriate depends on the type of biological sample on hand.

     Pielou (1966) classifies the types of biological samples and gives  the
measure of diversity appropriate for each type.  The Survey phytoplankton
samples are what she classifies as larger samples (collections in Pielou's
terminology) from which random subsamples can be drawn.  According to Pielou,
the average diversity per individual for these types of samples can be
estimated from the Shannon-Wiener formula (Shannon and Weaver 1963):



                         H  =  J  P. logx P.
where P is the proportion of the ith taxon in the sample,  which is calculated
from n./N; n. is the number of individuals per milliliter  of the ith taxon;
N is the total number of individuals per ml; and S is the  total number of
taxa.

     However, Basharin (1959) and Pielou (1966) have pointed out that H
calculated from the subsample is a biased estimator of the sample H, and if
this bias is to be accounted for, we must know the total number of taxa
present in the sample since the magnitude of this bias depends on it.

     Pielou (1966) suggests that if the number of taxa in  the subsample falls
only slightly short of the number in the larger sample, no appreciable error
will result in considering S, estimated from the subsample, as being equal  to
the sample value.  Even though considerable effort was made to find and
identify all taxa, the Survey samples undoubtedly contain  a fair number of
rare phytoplankton taxa which were not encountered.

-------
     In the Shannon-Wiener formula, an increase in the number of taxa  and/or
an increase in the evenness of the distribution of individuals among  taxa
will increase the average diversity per individual from its minimal value  of
zero.  Sager and Hasler (1969) found that the richness of taxa was  of  minor
importance in determination of average diversity per individual for phytoplank-
ton and they concluded that phytoplankton taxa in excess of the 10  to  15 most
abundant ones bave little effect on H, which was verified by our own  calcula-
tions.  Our counts are in number per milliliter and since logarithms  to the
base 2 were used in our calculations, H is expressed in units of bits  per
individual.  When individuals of a taxon were so rare that they were  not
counted, a value of 1/130 per mililiter or 0.008 per mililiter was  used in
the calculations since at least one individual of the taxon must have  been
present in the collection.

     A Survey sample for a given lake represents a composite of all phytoplank-
ton collected at different sampling sites on a lake during a given  sampling
period.  Since the number of samples (M) making up a composite is a function
of both the complexity of the lake sampled and its size, it should  affect  the
richness-of-taxa component of the diversity of our phytoplankton collections.
The maximum diversity (MaxH) (i.e., when the individuals are distributed
among the taxa as evenly as possible) was estimated from log? S, the  total
diversity (D) was calculated from HN, and the evenness component of diversity
(J) was estimated from H/MaxH (Pielou 1966).  Also given in the Appendix are
L (the mean number of individuals per taxa per millilHer) and K (the number
of individuals per milliliter of the most abundant taxon in the sample).

     Zand (1976) suggests that diversity indices be expressed in units of
"sits", i.e., in logarithms to base S (where S is the total number of taxa in
the sample) instead of in "bits", i.e., in logarithms to base 2.  Zand points
out that the diversity index in sits per individual is a normalized number
ranging from 1 for the most evenly distributed samples to 0 for the least
evenly distributed samples.  Also, it can be used to compare different
samples, independent of the number of taxa in each.  The diversity in bits
per individual should not be used in direct comparisons involving various
samples which have different numbers of species.  Since MaxH equals log S,
the expression in sits is equal to logs S, or 1.  Therefore diversity in sits
per individual is numerically equivalent to J, the evenness component for the
Shannon-Wiener formula.

SPECIES OCCURRENCE AND ABUNDANCE

     The alphabetic phytoplankton species list for each lake, presented in
the Appendix, gives the concentrations of individual species by sampling
date.  Concentrations are in cells, colonies, or filaments (CEL, COL, FIL)
per milliliter.   An "X" after a species name indicates the presence of the
species on that date in such a low concentration that it did not show up in
the count.   A blank space indicates that the organism was not found in the
sample collected on that date.  Column S is used to designate the examiner's
subjective opinion of the five dominant taxa in a sample, based upon relative
size and concentration of the organism.  The percent column (%C) presents, by
abundance,  the percentage composition of each taxon.
                                      8

-------
                                LITERATURE CITED
Basharin, 6.  P.   1959.   On a statistical estimate for the entrophy of a
     sequence of independent random variables, pp. 333-336.   In N. Artin
     (ed.). Theory of Probability and Its Applications (translation of
     "Teoriya Veroyatnose i ee Premeneniya") 4.   Society for Industrial and
     Applied Mathematics, Philadelphia.

Brillouin, L.  1962.  Science and Information Theory (2nd ed.).  Academic
     Press, New York.  351 pp.

Hutchinson, G. E.  1967.  A Treatise on Limnology.  II.   Introduction to Lake
     Biology and the Limnoplankton.  John Wiley and Sons, Inc., New York.
     1,115 pp.

Nygaard, G.  1949.  Hydrobiological studies of some Danish ponds and lakes.
     II.  (K danske Vidensk. Selsk.)  Biol. Sci. 7:293.

Palmer, C. M.  1969.  A composite rating of algae tolerating organic pollution.
     J. Phycol.   5:78-82.

Pielou, E. C.  1966.  The measurement of diversity in different types of
     biological  collections.  J. Theor.  Biol.  13:131-144.

Prescott, G. W.   1970.   How to Know the Freshwater Algae.  William C. Brown
     Company, Dubuque.   348 pp.

Sager, P. E. and A.D. Hasler.  1969.  Species diversity in lacustrine phyto-
     plankton.  I.  The components of the index of diversity from Shannon's
     formula.  Amer. Natur.  103(929):51-59.

Shannon, C. E. and W. Weaver.  1963.  The Mathematical Theory of Commun-
     ication.  University of Illinois Press, Urbana.  117 pp.

U.S. Environmental Protection Agency.  1975.  National Eutrophication Survey
     Methods 1973-1976.  Working Paper No. 175.   Environmental Monitoring and
     Support Laboratory.  Las Vegas, Nevada, and Corvallis Environmental Re-
     search Laboratory, Corvallis, Oregon.  91 pp.

Zand, S. M.  1976.  Indexes associated with information theory in water
     quality.  Journal  WPCF.  48(8)=2026-2031.

-------
                     APPENDIX.   SUMMARY OF PHYTOPLANKTON DATA


     This appendix was generated by computer.   Because it was only possible
to use upper case letters in the printout, all  scientific names  are printed
in upper case and are not italicized.

     The alphabetic phytoplankton lists include taxa without species names
(e.g., EUNOTIA, EUNOTIA #1, FLAGELLATE, FLAGELLATES, MICROSYSTIS INCERTA ?,
CHLOROPHYTAN COCCOID CELLED COLONY).   When species determinations  were not
possible, symbols or descriptive phrases were  used to separate taxa for
enumeration purposes.  Each name on a  list, however, represents  a  unique
species different from any other name  on the same list,  unless otherwise
noted, for counting purposes.

     Numbers were used to separate unidentified species  of the same genus.   A
generic name listed alone is also a unique species.   A question  mark (?) is
placed immediately after the portion of a name which was assigned  with uncer-
tainty.  Numbered, questioned,  or otherwise designated taxa were established
on a lake-by-lake basis; therefore NAVICULA #2 from lake A cannot  be compared
to NAVICULA #2 from lake B.  Pluralized categories (e.g., FLAGELLATES, CENTRIC
DIATOMS, SPP.) were used for counting  purposes when taxa could not be properly
differentiated on the counting  chamber.
                                      10

-------
LAKE NANE: LAKE CUMBERLAND
STCPET NUMBER: 2101
                                              NYGAARD TROPHIC STATE INGICES

                                        DATE   05 25 73  08 21 73  10 25 73
MYXOPHYCEAN
CHLOROPHYCEAN
EUGLENCPHYTE
DIATOM
COMPOUND
02/0 F
01/0 E
0/03 ?
0.30 E
07/0 E
3.00 E
1.00 E
0.25 E
0.25 ?
6.00 E
03/0 E
Cl/0 E
0.25 E
0.33 E
07/0 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   05 29 73  08 21 73  10 25 73
                                       GENUS
                                     SPECIES
00
00
03
00
03
00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES

                                        DATE   05 29 73  08 21 *3  10 25 73
                      AVERAGE DIVERSITY
                         NUMBER OF TAXA
           NUMBER OF SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                        TOTAL DIVERSITY
         TOTAL NUMBER OF INDIVIDUALS/ML
                      EVENESS COMPONENT
        MEAN NUMBER OF INDIVIDUALS/TAXA
       NUMBER/ML OF MOST ABUNDANT TAXON
H
S
M
H
0
N
J
L
K
2.29
17.00
7.00
4.09
2665.32
1169.00
0.56
68.76
447.00
1.13
15.00
7.00
3.91
7133.69
6313.00
0.29
420.37
5132.00
2.34
17.00
7.00
4.09
2686.32
1148.00
0.57
67.53
628.00

-------
            LAKE NAME: IAKE CUMBERLAND
            STCRET NUMBER: 2101
                                   CONTINUED
ro
                                                              05  29  73
                                                                  08 21 73
                                                                                               10 25 73
TAXA

ANABAENA
ANABAEKA #1
ANABAENA #2
ANCMCECNEIS
APHANOTHECE
ASTERICNELLA FORMOSA
CERATIUM H1RUNOINELLA
CCSMARIUM
CRYPTOMONAS
CYANOPHYTAN FILAMENT
CYCLOTELLA
CYMBELLA
DINCBRYCN
DINOBRYON DIVERGENS
DINCFLAGELLATE #1
DINOFLAGELLATE #2
FLAGELLATES
FPAGILARIA CROTCNENSIS
MALLCMCNAS PSEUOOCORONATA
MELOSIRA #2
MELOSIRA 01STANS
MELOSIRA VAR1ANS
NAVTCULA
MAVICULA SALINAR1UM
 V. INTERMEDIA
NITZSCHIA
OSCILLATORIA
CSCILLATORIA #1
PANDOR1NA MQRUM
PHACLS
SCENEDESMUS
STEPHANOOISCUS
FORM
F!L
FIL
FIL
CEL
COL
CEL
CEL
CEL
CEL
FIL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
FIL
FIL
COL
CEL
COL
CEL
ALGAL
UNITS
S tC PER ML








4




5


2
1

















1.1


6.8




9.0


38.2
33.7



2.2




1.1



X




13
X

79




105


447
394

X
X
26
X
x ^


13
X


3| 5.6! 66
ALGAL
UNITS
S ?C PER ML



1



3


4





2
5















0.31 19
\
81.31 5132
0.31 19


0.31 19
3.9| 248
1
0.31 19
2.11 133





9.1
0.9















X
572
57
X



X






0.31 19


ALGAL
UNITS
S *C PER ML



I






4





3
5














X
X
54.7 628






5.3| 61

X
1.31 15
1
2.7! 31
1
9.31 107
8.01 92
i.3| 15
4.01 46





1.3









X
15


X



-------
LAKE NANE: LAKE CUMBERLAND
STORET NUMBER: 2101
CONTINUED
TAXA

SYNECRA #1
SYNEDPA DELICATISSIKA
 V. ANGUSTISSIMA ?
TETRAEDRCN KINIMUM
TRACHELOMONAS
                                                  05 29 73
                                08  21  73
                                                                                    10  25


FORM
CEL

CEL
CEL
CEL


S *C
1 1.11
1 1
1 1
1 1.11
1 1
ALGAL
UNITS
PER ML
13


13



S *C
1 0.91
1 1
! 1
1 1
1 0.31
ALGAL
UNITS
PER ML
57



19


S
21
1
1
1
1
ALGAL
UNITS
%C PER ML
9.31 107
1
1 x
2.71 31
1
  TOTAL
                 1169
6313

-------
LAKE NAME: DALE HOLLOW RE*.
STCRET NUMBER: 2102
                                              NYGAARD TROPHIC STATE INDICES

                                        DATE   05 18 73  08 18 73  10 24 73
                                 MYXOPHYCEAN
                               CHLOROPHYCEAN
                                EUGLENOPHYTE
                                      DIATOM
                                    COMPOUND
04/0 E
01/0 E
0.20 ?
0.40 E
08/0 E
0/0  0
02/0 S
0/02 ?
1.00 E
06/0 E
01/0 E
04/0 E
0/05 ?
0/05 ?
05/0 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   05 IS 73  08 18 73  10 24 73
                                       GENUS
                                     SPECIES
    00
    00
    01
    00
    00
    00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES

                                        DATE   05 18 73  08 18 73  10 24 73
                      AVERAGE DIVERSITY    H
                         NUMBER OF TAXA    S
           NUMBER OF SAMPLES COMPOSITED    M
                      MAXIMUM DIVERSITY MAXH
                        TOTAL DIVERSITY    D
         TOTAL NUMBER OF INDIVIDUALS/ML    N
                      EVENESS COMPONENT    J
        PEAN NUMBER OF INDIVIDUALS/TAX A    L
       NUMBER/ML OF MOST ABUNDANT TAXON    K
2.12
16. 00
6.00
4.00
493.56
233.00
0.53
14.56
112.00
2.28
17.00
6.00
4.09
2948.04
1293.00
0.56
76.06
817.00
2.13
14.00
6.00
3.81
1433.49
673.00
0.56
48.07
4U.OO

-------
LAKE NAME: DALE HOLLCH RES.
STCRET NUMBER:  2102
CONTINUED
                                                  05 18 73
                                08 18 73
                                                                                    10 24 73
TAXA

ACHMANTHES MICROCEPHALA ?
ANA6AENA
ANCMCECNEIS VITREA
ASTERICNELLA FORMOSA
CEMTRIC DIATOM ^~


2








*



3




I
5









1
1.31 3
1
6.M 15
1
2.11 5
1 X
1
1
1
1
1
4.31 10
1 X
l.3| 3
1
30.51 71
1
1
1 X
1
48.11 112
2.11 5
1. 3 1 3



X
1.3 3
1.3 3


ALGAL
UNITS
S *C PER ML


1
2







4





3



5










1
1
63.21 817
4.61 59
0.9| 12
1
1.91 24


1.9

6.4



1.9

3.6
1.9


5.5


0.9
1.9






24

S3



24

47
24


71


12
24
X



1.91 24
0.9 1 12
ALGAL
UNITS
S *C PER ML


3




4












5
I










3.9| 26
1
7.3f 49




7.3
1.3

2.5









5.2
61.2


1.3









49
9

n









35
412
X

9





1.31 9
1.3f 9

-------
LAKE NAME: DAL* t-OLLCW RES,
STRET NUMBER: 2102
CONTINUED
TAXA

SCENEDESMUS #3
SYNECPA
SYNEORA #1
SYNECPA #2

  TOTAL
                                                   05  18  73
                                08 18 73
                                                                                     10  24  73


FORM
COL
CEL
CEL
CEL

1 ALGAL
1 UNITS
IS *C PER ML
T 1 1
1 1 1
1 1 1
II 1 X
233
ALGAL
UNITS
S ?C PER ML
1 1
1 1
1 1.91 24
1 0.91 12
1293
1 ALGAL
I UNITS
IS %C PER ML
III X
|2| 7.3| 49
III X
1 1 1
673

-------
LAKE NAME: HERRINGTON LAKE
STCRET NUMBER: 2103
                                              NYGAARC TROPHIC STATE  INDICES

                                        DATE   05 26 73  08  20 73  10 23  73
                                 MYXCPHYCEAN
                               CHLOROPHYCEAN
                                EUGLENOPHYTE
                                      DIATCM
                                    COMPOUND
04/0 E
05/0 E
0/09 ?
0.60 E
12/0 E
10.0 E
3.00 E
0.03 ?
0.25 ?
15.0 E
03/0 E
OS/0 E
0.27 E
0.40 E
18/0 E
                                            PALMER'S ORGANIC POLLUTION  INDICES

                                        DATE   05 26 73  08 20 73  10 23 73
                                       GENUS
                                     SPECIES
    11
    00
    04
    10
    00
                                         SPECIES DIVERSITY AND ABUNDANCE  INDICES

                                        DATE   05 26 73  08 20 73  10 23  73
                      AVERAGE DIVERSITY
                         NUMBER OF TAXA
           NUMBER OF SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                        TOTAL DIVERSITY
         TOTAL NUMBEP OF INDIVIDUALS/ML
                      6VENESS COMPONENT
        MEAN NUMBER CF INDIVIDUALS/TAXA
       NUMBER/ML OF POST ABUNDANT TAXCN
H
S
M
H
D
N
J
L
K
1.48
21.00
4.00
4.39
15696.88
10606.00
0.34
505.05
4970.00
2.90
24.00
4.00
4.58
16173.30
5577.00
0.63
232.38
1711.00
1.70
32.00
4.00
5.00
17715.70
10421.00
0.34
325.66
7501.00

-------
           LAKE NAME: HARRINGTON  LAKE
           STCRET NUMBER: 2103
                                   CONTINUED
00
TAXA

ACHNANTHES MICROCEPHALA ?
ACTINASTRUM HANTZSCt-II
AMFHCRA
ANABAENA
ANABAENA #1
ANAEfAENCPSIS
ASTERICNELLA FORMOSA
COELASTRUM NICRCPORUM
COELASTRUM SPHAERICUI*
COSMARIUM
CYCLOTELLA
CYCLOTELLA STELLIGERA
CACTYLOCOCCOPSIS
DINCFLAGELLATE
DINOFLAGELLATE #1
CINOFLAGELLATE t2
EUGLENA #1
EUGLENA #2
FLAGELLATES
FRAGILARIA CRDTOMENSIS
GCNIUM PECTORALE
HANTZSCHIA
KIRCHNERIELLA
LYNGBYA LIMNETICA
fELCSIRA #2
MELOSIRA #A
t'ELOSIRA DISTAMS
MERISMOPEOIA HAPSSONII
MERISMOPEOIA TENUISSIMA
MESOSTIGMA
fICRGCYSTIS INCERT»
NAVICULA #1
                                                             05 26 73
                                                                   08 20 73
10 23 73
FORM
CEL
COL
CEL
Fit
FIL
FIL
CEL
COL
COL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CCL
CEL
CEL
FIL
CEL
CEL
CEL
CCL
COL
CEL
COL
CEL
S






4











I
2




5







*C



0.2


0.8



0.3

0.2
0.2




46.9
46.9




0.6







ALGAL
UNITS
PER ML



22


89
X


89

22
22




4970
4970
X



67







S




5













1













*C




4.3
0.5


0.3
0.3


1.5

0.8


0.3
26.9




0.5




1.3

0.5

ALGAL
UNITS
PER ML



X
2«2
29


14
14


86

43
X

14
1498




29




71

29

S











4






I







2
5




*C

1,7









2.4
1.7



0.7
0.3)
72.0



0.3


1.4
9.3
2.0
1
1.0

1
ALGAL
UNITS
PER ML
X
1*5
X





X


246
176

X
X
70
35
7501


X
35


141
1021
211

106
1 X
X

-------
LAKE NAME: HERRTNGTON LAKE
SECRET NUMBER: 2103
CCNTINUED
                                                  05 26 73
                                CB 20 73
                                                                                    10 23 73
TAXA

NAVICULA #2
NITZSCHI* 7 02
NITZSCH1A #1
NITZSCHIA HOLSATICA ?
OSCILLATORIA
OSCILLATOR I A LIMNETICA
PAKDORINA NCRUM
PHACUS ALATUS ?
PAPHIDIOPS1S
SCENEOESMUS #1
SCENEDESHUS #2
SCENEDESMUS #3
SCENEOESMUS DIMORPHIS
SCENEDESMUS QUACRICAUDA
STEPHANCOISCUS
STEPHANOOISCUS ?
SUPIRELLA
SYNECRA #1
SYNEDRA #2
SYNE DP A #3
SYNEDRA DELICAT1SS1MA
TETRAEDRCN MINTMUM
TETPAEOPCN MUT1CUM

  TCTAL
FORM
CEL
CEL
CEL
CEL
CEL
FIL
FIL
COL
CEL
FIL
COL
COL
COL
COL
CCL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
ALGAL
UNITS
S *C PER ML















3














0.6



0.8
0.4

0.2

1.3








X


X

X
67
X


39
44

22

133





X

X
ALGAL
UNITS
S K PEP ML






2


4






3







1
1
1
1
1.51 S6
1 X
10.51 585
1 X
1
30.71 1711
1
I
1
1
1.8) 100
1
11.51 642
1
4.3| 242
1
1.01 57
0.31 14
1.31 71
1
ALGAL
UNITS
S %C PER ML















3








1 X
0.71 70
I X





C.3


0.3



5,4













35

X
35
X
X

563

X
X
X


X

10606 5577 10421

-------
LAKE NAME: KENTUCKY LAKE
STCPET NUMBER: 2104
                                              NYGAARD TPQPHIC STATE INDICES

                                        DATE   05 16 73  08 13 73  10 20 73
                                 MYXOPHYCEAN
                               CHLOROPHYCEAN
                                EUGLENOPHYTE
                                      DIATOM
                                    COMPOUND
 4.00 E
 8.00 E
 0.33 E
 1.00 E
 24.0 E
1.33 E
2.67 f
0.25 £
1.20 E
6.00 e
1.43 E
1.57 E
0.14 ?
1.17 E
4.43 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        CATE   05 16 73  08 13 73  10 20 73
                                       GENUS
                                     SPECIES
     04
     00
    15
    02
    17
    01
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE
                      AVERAGE DIVERSITY
                         NUMBER OF TAXA
           NUMBER OF SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                        TOTAL DIVERSITY
         TOTAL NUMBER OF INDIVIDUALS/ML
                      EVENESS COMPONENT
        MEAN NUKBER CF INOIVIDUALS/TAXA
       NUMBER/ML OF MOST ABUNDANT TAXON
05 16 73  08 13 73  10 20 73
H
S
M
;H
D
N
J
L
K
2.37
39.00
17.00
5,29
13049.22
5506.00
0.45
141.18
2225.00
3.64
52.00
10.00
5.70
21752.64
5976.00
0.64
114.92
1002.00
3.42
46.00
9.00
5.52
26555.72
7766.00
0.62
168.83
2943.00

-------
LAKE NAME: KENTUCKY LAKE
STORET NUMBER:  210*
CONTINUED
                                                  05 16 73
                                09 13 73
                                                                                    10  20  73
TAXA

ACHNANTHES MICROCEPHALA ?
ANABAENA #1
AKABAENA #2
ANABAENA #3
ANABAENOPSIS
ANABAENOPSIS PHIL!PPINENS1S
ANKISTP.QDESMUS
APHAMZCPENCN
APHANIZCMENCN ?
ASTERICNELLA FORMOSA
CENTRITRACTUS ?
CERATIUM HIRUNDINELLA
CLCSTERIOPSIS ?
CLCSTERIUM ? #1
CLCSTERIUH 7 #3
CLCSTERIUM #2
COPLASTRUM RETICULATUM
COELASTRUM SPHAERICUM
CCSMARIUM
CCSMARIUM #1
CCSHARIUM «2
CRUCIGEMA APICULATA
CRYPTOMCNAS
CYCLOTELLA fENEGHINIANA
CYCLOT^LLA STELLIGERA
CYMBELLA
DACTYLPCOCCOPSIS
DICTYOSPHAERIUM PULCHELLUM
DINCBRYON
DINCBRYCN EAVARICUM
DINOFLAGELLATE
DINOFLAC-ELLATES
FORM
CEL
FIL
FIL
FIL
FIL
FIL
CEL
COL
FIL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
COL
COL
CEL
CEL
CEL
COL
CEL
CEL
CEL
CEL
CEL
COL
CEL
CEL
CEL
CEL
S
































*c









0.8













0.4
0.2

0.5

0.1



ALGAL
UNITS
PER ML
"








42
X

X




X





20
11
X
30

6
X

X
S
































%c

1.5



5.4

0.5











0.5



3.0






0.5)

ALGAL
UNITS
PEP ML

I 88



324
X
29



X

X

X
X
X

29
X
X
X
177



X


29

S

4






























*C
1.6
6.6
1.1
0.6
0.6
0.6
0.6

2.2








0.6





0.6
1.1







ALGAL
UNITS
PER ML
1 128
512
1 35
1 43
I 43
1 43
1 43

1 171





X
X

43
X


X

43
85


X


X


-------
           LAKE  NAME:  KENTUCKY  LAKE
           STOPET  DUMBER:  2104
                                   CONTINUED
                                                            05 16 73
                                                                  08  13 73
10 20 73
ro
ro
TAXA

EUASTRUM DENTICUIATUM
EUASTRUM DENTICULATUM ?
EUCLENA
EUGLENA #1
EUGLENA #2
EUGLENA #3
FLAGELLATES
GYRC SIGMA
GYPOSIGNA 7
LYNGBYA HZ
LYNGBYA CCNTORTA
MALLCMONAS PSEUCCCCRONATA ?
MELOSIRA #2
MELOSIRA #3
MELOSIPA #4
KELCSIRA #5
MELOSIRA OISTANS
MELOSIRA VARIANS
MERISMOPEDIA
MEPISMOPEDIA MARSSONI!
MICRACTINIUM
MICROCYSTIS AERUGINOSA
NAVICULA
NITZSCHIA
NITZSCHIA #2
NITZSCHIA ACTCULARIS
OSCILLATCRIA
OSCILLATORIA LIMNETICA
PANDORINA MORUM
PEOIASTRUM BlRACtATUf
 V. LCINGECORNUTUN
PEDIASTPUI" SIMPLEX
 V. DUOOENARIUM
FORM
COL
COL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
FIL
FIL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
COL
COL
COL
COL
CEL
CEL
CEL
CEL
FIL
FIL
COL
COL
COL
s






2





3

1

5














*C






31.3




1.0
9.1

40.4

4.5
0.4
0.5




1.3



0.1



ALGAL
UNITS
PER ML

X
X



1753
X



1 56
1 503
1
1 2225

I 250
1 21
1 28

X

X
73


X
6



S






2





3
5
I












4



?C
0.5


0.5


15.3


12.3)
0.51
1
7.9
3.91
IS. 8
1
5.4


0.5

1.0
0.5

1.5


14.8



ALGAL
UNITS
PER ML
29


29
X
X
913

X
737
29

471
236
1002

324


29

59
29

88
X

884
X
X
X
s






3





2












5

1



*C






11.0


0.6
0.6
1
8.8

4.4
1.6
i.r


0.6


1.1

4.4
6.6

37.9



ALGAL
UNITS
PER ML
X



X

953


43
43

683

341
128
85


43


85

341
512

2943


X

-------
           LAKE  NAME:  KENTUCKY LAKE
           STCRET  NUMBER:  2104
                                   CONTINUED
r\s
                                                             05 16 73
                                                                   03  13  73
                                                                                               10 20 73
TAXA

PEDIASTRUM TETRAS
 V. TFTRAOCON
PENNATE CIATOM
PHACUS
PHACUS PYRUM
PHACUS 7CRTUS
RHIZOSOLENIA
SCENEDESMUS
SCENEDESMUS #1
SCENEDESMUS #2
SCENEDESML'S A8UNCANS
SCENEDESMUS BIJUGA
 V. ALTERNANS
SCENEDESKUS DIMCPPHUS
SCENEDESMUS DISPAR
SCENEDESPUS PROTUBERANS
SCENEDESMUS QUADRICAUDA
SCENEDFSMUS QUADRICAUDA  ?
 V. ALTERNANS
SCHROEDERIA SETIC-ERA
STAURASTRUM #1
STAURASTRU* #2
STAURASTRUM #3
STEPHANODISCUS
SURIRELLA ANGUSTATA
SYNEDRA
SYNEDRA #1
TETRAEOPCN VINIMUM
 V. SCROBICULATUH
TETRAEDRCN TRIGCNUM
 V. G"ACUE
TETRASTRUM ELEGANS
FORM
COL
CEL
CEL
CEL
CEL
CEL
COL
COL
COL
COL
COL
COL
COL
COL
COL
COL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
COL
S




















*






%C

1.5




0.3








0.1




6. A


0.5



ALGAL
UNITS
PER ML

93

X

X
14


X



X

6




354
X

25


X
S



























?c







1.0
0.5





0.5

0.5



4.9





1
ALGAL
UNITS
PEP ML
X

X




59
29

X
X
X

29

29

X

295


X

X

S



























%c











1.1


0.6





2.7


0.6
0.6


ALGAL
UNITS
PEP ML




X


X
X

X
65


43


X
X
X
213

X
43
43



-------
ro
            LAKE NAME: KENTUCKY LAKE
            STCPET NUMBER: 2104
                                   CONTINUED
TAXA

TRAC^LOMONAS #1
TRACHELCMOKAS <*2
                                                              05 16 73
                                                                   08 13 73
10 20 73


FORM
CEL
CEL
1
1
IS
1 1
1 1
ALGAL
UNITS
*C PER ML
1 X
1 X
1
1
IS
1 1
1 1
ALGAL
UNITS
*C PER ML
1 X
1 x
1 ALGAL
1 UNITS
IS *C PER ML
III X
1 1 1
1
1
1
1
1
              TOTAL
                                                                5506
                                                                     5976
  7766

-------
                   LAKE  NAPE:  BARPEK  RIVER  RES.
                   STORET  NUMBER:  2105
                                                                 NYGAARO TRCPHIC STATE INDICES

                                                           DATE   05 18 73  08 11 73  10 23 73
                                                    MYXOPHYCEAN
                                                  CHLORCPHYCEAN
                                                   EUGLENCPHYTE
                                                         DIATOM
                                                       COMPOUND
02/0 E
07/0 E
0.11 ?
0.80 E
18/0 E
04/0 E
01/0 E
0.80 E
0.50 E
14/0 E
2.00 E
4.67 E
0.20 ?
0.71 E
9.67 E
ro
en
                                                               PALMER'S ORGANIC POLLUTION INDICES

                                                           DATE   05  18 73  08 11 73  10 23 73
                                                          GENUS
                                                        SPECIES
    07
    00
    16
    00
    11
    04
                                                            SPECIES DIVERSITY AND ABUNDANCE INDICES

                                                           DATE   05 18 73  08 11 73  10 23 73
                                         AVERAGE DIVERSITY
                                            NUMBER OF TAX A
                              NUMBER OF SAMPLES  COMPOSITED
                                         MAXIMUM DIVERSITY MAXH
                                           TOTAL DIVERSITY
                            TCTAL  NUMBER OF INDIVIDUALS/ML
                                         EVENESS COMPONENT
                           MEAN NUMBER OF INDIVIDUALS/TAXA
                          NUMBER/ML  OF MOST ABUNDANT TAXON
H
S
M
;H
D
N
J
L
K
2.68
34.00
11.00
5.09
23043.52
10464.00
0.53
307.76
4402.00
1.71
25.00
11.00
4.64
24871.95
14545.00
0.37
581.80
9775.00
3.25
45.00
10.00
5.49
10959.00
3372.00
0.59
74.93
968.00

-------
            LAKE NAME:  BARREN  RIVER R=S.
            SECRET NUMBER: 2105
                                   CONTINUED
tv
cn
TAX A

ACHNANTHES
ACHNANTHES LANCECLATA
ACHNANTHES MICRCCEPHAkA ?
ACTINASTRUP HANTZSCHII
ANABAENA
ANABAENA ?
ASTERIONELLA FORMOSA
CARTERIA
CERATIUM HIRUNDINELLA
 F. BRACHYCERAS
CHLOROGONIUM
CHPOOCOCCUS
COCCONEIS
COELASTRUM MICROPORUM
COELASTPUM RETICULATUM
CCSHARIUM
CRUCIGENIA APICULATA
CRUCIGEMA TETRAPEDIA
CRYPTOMONAS
CYCLOTELUA ATOMUS
CYCLOTELLA PENEGHINIANA
CYCLOTELLA MENEGHINIANA ?
CYCLOTELLA STELLIGERA
CYPBELLA
CYMBELLA AMPHICEPHALA
CACTYLOCCCCOPSIS
DINOBRYON BAVARICUM
DINOBRYON OIVERGENS
CINOBRYCN SOCIALE
 .V. AMfRICANUM
CINOFLAGELLATE
CIW3FLAGELLATE #2
                                                              05 18 73
                                                                  08  11 73
                                                                                                10 23 73
FORM
CEL
CEL
CEL
COL
F!L
FIL
CEL
CEL
CEL
CEL
COL
CEL
COL
COL
CEL
COL
COL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL

CEL
CEL
CEL
S































%c



0.3


0.3









0.3
4.4



2.7




0.6

3.0

1
ALGAL
UNITS
PER ML

X

32

X
32
X



X




32
255
X

X
287

X


1 64

319
1

S


3


















5









*C


9.2










0.2







1.4









ALGAL
UNITS
PER ML

X
1333

X








30





X

207
X








S





















4









*C
0.4







0.4





2.3
0.41
0.4




5.4


5.4
0.4





ALGAL
UNITS
PER HL
15



X



15
X
X

X

76
15
15




181
X

181
15



X
X

-------
            LAKE  NAPE:  BARRFN RIVER  RES.
            STORE!  NUMBER:  2105
                                   CCNTINUED
ro
                                                              05  18  73
                                                                   08 11 73
10 23 73
TAXA

EUASTRUN
EUGLENA
EU6LENA #1
EUGLENA 92
EUNOTIA
FLAGELLATES
GYRCSIGMA #1
GYROSIGMA #2
LYNGBYA LIMNETICA
HELOS1RA #2
MELOSIRA #4
PELOSIRA #5
MELOSIRA DISTANS
MELOSIRA VARIANS
PICRACTINIUM ?
NAVICULA #1
NAVICULA ANGLICA
 V. SU8SALSA
NAVICULA TRIPUNC7ATA
NITZSCHIA
NITZSCHIA #2
NITZSCHIA HOLSATICA ?
OSCILLATCRIA
CSCILLATORIA GEHINATA
OSCILLATQRIA LIMNET1CA
PAKDORINA f»ORUM
PEDIASTRUM 6IRAOIATUK
PECIASTRUH SIMPLEX
PEDIASTRUM SIMPLEX
 V. DUODENARIUM
PENNATE DIATOM
PENNATE DIATOM #3
FORM
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
FIL
CEL
CEL
CEL
CEL
CEL
COL
CEL
CEL
CEL
CEL
CEL
CEL
FIL
FIL
FIL
COL
COL
COL
COL
CEL
CEL
S





1



2
5







«











*C



0.3

42. 1



12.2
4.0

3.7





6.1


0.3








ALGAL
UNITS
PER ML



32
X
4402



1276
415

383
X
X


X
638


32


X



X

S





4


2





















*C

0.4



6.1


12.2



0.2





0.6



0.4







ALGAL
UNITS
PER ML
1
1 59



839
X
X
1777
X


30


X
X

89



59







S





2



3


1







5









*C


0.4


27.8


0.4
5.4


28.7







3.1


0.9





1.8
ALGAL
UNITS
PER HL
X

15
X

938


15
181

X
963






X
106


30

X
X
X

61

-------
             LAKE NAME:  BARREN RIVER RES.
             STORET  NUMBER:  2105
                                   CONTINUED
1X3
CO
                                                               05 18 73
                                                                   08 11 73
W 23 73
TAXA

PHACLS
PAPHIDIOPS1S ?
SCENEDESMUS #1
SCENEOESMUS #2
SCENEDESMUS ABUNCANS
SCENEDESfUS OENTICULATUS
SCENEDESMUS DIMORPHUS
SCENEDESMUS GUADRTCAUCA
STAURASTRUM
STEPKANODISCUS
SYNEDRA ? #1
SYNEDRA #1
SYNEDRA OELKATISSIMA
SYNECRA CELICATISSIMA ?
TE7RAEDRON
TETRAEDPON MINIMUM
TETRAEDRCN TRIGOf-UM
 V. GRACILE
TETPASTRUM STAUPQGENIAEFORME
TPACHELCMONAS
TRACHELCMONAS #1
TRACHELCMONAS SCHAUINSLANDII
TRACHELCMONAS VOLVOCINA
FORM
CEL
FIL
COL
COL
COL
CCL
COL
COL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL

CEL
COL
CEL
CEL
CEL
CEL
ALGAL
UNITS
S *C PER ML









3















0.3






20.1

0.6
0.6












32
X



X

2105

64
64





X




ALGAL
UNITS
S S5C PER ML



1|67.2| 9775




























0.6

0.6

0.4






0.2







89

89

59






30
0.21 30
1 X
ALGAL
UNITS
S ?C PER ML























1 X
4.51 151
1
1 x
1 x
0.41 15
1 x
2.31 76
1 X
0.41 15
1.81 61
1
2.7) 91
1
1 x
3.61 121
1
1 x
1
0.4| 15
1
1
1
               TOTAL
                                                                10464
                                                                    14545
                                                                                                  3372

-------
                                    TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before completing}
  . REPORT NO.
   EPA-600/3-78-013
                             2.
                                                           3. RECIPIENT'S ACCESSION NO.
 4. TITLE AND SUBTITLE


   DISTRIBUTION OF  PHYTOPLANKTON  IN  KENTUCKY LAKES
                                                           5. REPORT DATE
                                                             January 1978
                                                           6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
  W.D. Taylor,  F.A.  Hiatt, S.C.  Hern, J.W. Hilgert,
  V.W. Lambou,  F.A.  Morris, M.K.  Morris, L.R. Williams
                                                            8. PERFORMING ORGANIZATION REPORT NO.
 9. PERFORMING ORGANIZATION NAME AND ADDRESS
                                                            10. PROGRAM ELEMENT NO.
   Environmental  Monitorina and Support Laboratory
   Office of  Research and Development
   U.S. Environmental  Protection Agency
   Las Vegas,  NV  89114
                                                           11.
                                                                  ACT/GRANT NO.
 12. SPONSORING AGENCY NAME AND ADDRESS
   U.S. Environmental  Protection Agency-Las Vegas, NV
   Office of  Research  and Development
   Environmental  Monitoring and Support Laboratory
   Las Vegas,  NV   89114
                                                           13. TYPE OF REPORT AND PERIOD COVERED

                                                             03-07-73 to 11-14-73
                                                           14. SPONSORING AGENCY CODE
                                                              EPA/600/7
 15. SUPPLEMENTARY NOTES
   Previously  released in limited  distribution as No. 683  in  the Working Paper  Series
   for the  National  Eutrophication Survey.
 16. ABSTRACT
        This  is  a data report  presenting the species and  abundance of phytoplankton
   in the  5  lakes sampled by the  National Eutrophication  Survey in the State  of
   Kentucky.   Results from the calculation of several  water quality indices are
   also included (Nygaard's Trophic State Index, Palmer's Organic Pollution Index,
   and species diversity and abundance indices).
 7.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                               b.lDENTIFIERS/OPEN ENDED TERMS
                                                                         c. COSATI Field/Group
   *aquatic  microbiology
    lakes
   *phytoplankton
    water quality
                                                Kentucky
                                                lake eutrophication
                                                Nygaard's trophic  indice
                                                Palmer's organic pollu-
                                                tion indices
                                                Species diversity  and
                                                abundance indices
   06 C, M
   08 H
   13 B
 8. DISTRIBUTION STATEMENT


        RELEASE TO PUBLIC
                                              19. SECURITY CLASS (ThisReport)

                                                 UNCLASSIFIED
21. NO. OF PAGES

    36
                                              20. SECURITY CLASS (Thispage)
                                                 UNCLASSIFIED
22. PRICE
EPA Form 2220-1 (9-73)

-------