xvEPA
          United States
          Environmental Protection
          Agency
          Robert S. Kerr Environmental Research
          Laboratory
          Ada OK 74820
EPA-6 00/2-78-131a
June 1978
          Research and Development
Sewage Disposal on
Agricultural Soils:

Chemical and
Microbiological
Implications
          (Volume I
          Chemical Implications)

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                RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology. Elimination of traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:

      1.  Environmental Health  Effects Research
      2.  Environmental Protection Technology
      3.  Ecological Research
      4.  Environmental Monitoring
      5.  Socioeconomic Environmental Studies
      6.  Scientific and Technical Assessment Reports (STAR)
      7.  Interagency Energy-Environment Research and Development
      8.  "Special" Reports
      9.  Miscellaneous Reports

This report has  been assigned  to the ENVIRONMENTAL PROTECTION TECH-
NOLOGY series. This series describes research performed to develop and dem-
onstrate instrumentation, equipment, and methodology to repair or prevent en-
vironmental degradation from point and non-point sources of pollution. This work
provides the new or improved technology required for the control and treatment
of pollution sources to meet environmental quality standards.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                                    EPA-600/2-78-131a
                                                    June 1978
           SEWAGE DISPOSAL ON AGRICULTURAL SOILS:
          CHEMICAL AND MICROBIOLOGICAL IMPLICATIONS
                          VOLUME I

                    CHEMICAL IMPLICATIONS
                             by

L. R. Hossner, Chun-Wei Kao, R. W. Weaver, and J. A. Waggoner
              Soil and Crop Sciences Department
                    Texas A&M University
                College Station, Texas 77843
                      Grant No. R803281
                       Project Officer

                       Lowell E. Leach
                Wastewater Management Branch
      Robert S. Kerr Environmental Research Laboratory
                     Ada, Oklahoma 74820
      ROBERT S. KERR ENVIRONMENTAL RESEARCH LABORATORY
             OFFICE OF RESEARCH AND DEVELOPMENT
            U.S. ENVIRONMENTAL PROTECTION AGENCY
                    ADA, OKLAHOMA 74820

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                                 DISCLAIMER

      This report has been reviewed by the Robert S. Kerr Environmental Research
Laboratory, U.S. Environmental Protection Agency, and approved for publication.
Approval does not signify that the contents necessarily reflect the views and policies
of the U.S. Environmental Protection Agency, nor does mention of trade names
or commercial products constitute endorsement or recommendation for use.
                                     ii

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                                  FOREWORD

      The Environmental Protection Agency was established to coordinate administra-
tion of the major Federal programs designed to protect the quality of our environ-
ment.

      An important part of the agency's effort involves the search for information
about environmental problems,  management techniques, and new technologies
through which optimum use of the nation's land and water resources can be
assured and the threat pollution poses to the welfare of the American people can
be minimized.

      EPA's Office of Research  and Development conducts this search through a
nationwide network of research facilities.

      As one of these facilities, the Robert S. Kerr Environmental Research
Laboratory is responsible for the management of programs to:  (a) investigate
the nature, transport,  fate and  management of pollutants in groundwater; (b)
develop and demonstrate methods for treating wastewaters with soil  and other
natural systems;  (c) develop and demonstrate pollution control technologies for
irrigation return flows; (d)  develop and demonstrate pollution control technologies
to prevent, control or abate pollution from the petroleum refining and petrochemical  '
industries; and.(f)  develop and demonstrate technologies to manage pollution re-
sulting from combinations of industrial wastewaters or industrial/municipal waste-
waters .

      This report contributes to the knowledge essential if the EPA is to meet the
requirements of environmental laws that it establish and enforce pollution
control standards which are reasonable, cost effective and provide adequate
protection for the American public.
                                                  *i
                                                   ft
William C. Galegar
Director
Robert S. Kerr Environmental Research Laboratory
                                     ill

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                                  ABSTRACT
     The city of San Angelo, Texas has used sewage effluent, which has
undergone primary treatment, for irrigation of the same agricultural land
since 1958.  The impact of 18 years of sewage effluent irrigation on the
soil and water quality of the 259 hectare sewage farm was studied from 1975
to 1977.  The volume of sewage arriving at the sewage treatment plant ranged
from 0.219 to 0.351 nrVsec.  In 1976, sewage effluent contributed from 3.4
to 31.2 percent of the total flow of the Concho River, which borders the
sewage farm.

     The fate of applied nitrogen, phosphorus, and selected heavy metals in
the so11-water-plant ecosystem was examined.  Nitrogen and phosphorus were
being applied to the farm in large amounts.  The average concentrations of
nitrogen and phosphorus in lagoon No. 4 effluent were 22.2 yg N/ml and
9.83 yg p/ml, respectively.  The concentration of Zn, Cu, Ni, Cd, Cr and
Pb in the incoming sewage effluent was low.
                          f
     There was evidence of some NO--N accumulation in the soil profiles and
in the Concho River.  No accumulation of NH.-N was found in the soil
profiles.  Some organic nitrogen accumulated at the surface of the soil.
After 18 years of continuous irrigation with sewage wastewater there was
a net loss of total nitrogen in the soil profile.  Phosphorus accumulated in
the soil profile.  It is apparent that most of the phosphorus was retained
in the upper 200 cm of soil.  Significant increases in water soluble
phosphorus in the upper 200 cm of soil indicates a buildup of soluble forms
to that depth.  There was a slight accumulation of certain heavy metals in
the surface of the soils.  Generally, heavy metal contamination is not a
serious problem on the sewage farm.

     Water from deep wells located on the sewage farm had a nutrient com-
position similar to wells located in the surrounding area of Tom Green
County.  The results indicate that land disposal of municipal sewage efflu-
ent combined with a forage and cattle production system can be considered a
practical long-term treatment scheme.

     This report was submitted in fulfillment of Grant No. R803281 by
Texas A&M University under the sponsorship of the U.S. Environmental
Protection Agency.  This report covers the period April 28, 1975, to
June 27, 1977, and work was completed as of June 27, 1977.
                                     xv

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                                  CONTENTS
Foreword                                                                 ill
Abstract                                                                  iv
List of Figures                                                          vii
List of Tables                                                            ix
Acknowledgments

Sections

     1.   Introduction 	  1
     2.   Summary and Conclusions 	  3
     3.   Recommendations 	  5
     4.   General Project Characteristics 	  6
            Topography 	  6
            Geology	  8
            Groundwater 	  8
            City Population and Industrial Input 	 10
            Wastewater Characteristics 	 10
            Vegetation on Farm	 11
            Animal Population and Crop Production	 13
     5.   Methods and Materials 	 16
            Hydrology and Meteorology	 16
            Water Analysis 	 16
               Water Sampling Locations 	 16
               Chemical Analysis 	 20
            Soil Analysis 	 20
            Field Plot Experiment and Plant Analysis 	 20
            Statistical Analysis 	 23
     6.   Results and Discussion 	 24
            Hydrology and Meteorology 	 24
               Meteorology 	 24
               Hydrology 	 24
            Water Analysis 	 30
               Temperature 	 33
               pH	 33
               Nitrogen 	 33
               Phosphorus	 61
               Heavy Metals 	 63
               BOD and COD 	 65
               TFR and TNFR	 65
               Statistical Relationships between Water Parameters  	 66
            Plant Analysis and Field Plot Experiment 	 70
                                             *                   (continued)

                                      v

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      Soil Analysis
         Introduction 	  74
         Initial Characterization of Physical and
            Chemical Properties 	„	  76
         Texture	  76
         Soil pH	  76
         Organic Matter 	  78
         Carbonate	  78
         Cation Exchange Capacity 	  78
         Soil Salinity	  80
         Statistical Relationships between
            Surface Soil Properties 	  80
         1 N HC1 Extractable Heavy Metals	  80
      Nutrient Loading of Soil Profiles 	  82
         Total Nitrogen	  86
         Exchangeable Inorganic Nitrogen 	  86
         Total Phosphorus	  97
         Water Soluble Phosphorus 	  98
         Total Heavy Metals	 101
            Cadmium	 102
            Chromium	 102
            Copper	 105
            Lead	 105
            Nickel	 107
            Zinc	 107
7. References	 112
                            vi

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                                  FIGURES
Number                                                              Page
                                                     /
   1     General topography and location of study site at
            San Angelo , Texas ....................................     7

   2     Cross section of the geologic strata of Tom Green
            County , Texas ........ . ...............................
   3     Cross sections of three different types of levees
            present on the San Angelo sewage farm ................   12

   4     San Angelo sewage farm with blow up on Coastal
            bermudagrass fenced plot area ........................   17

   5     Average monthly precipitation for the years 1936-1975
            and monthly precipitation in 1976 at the San Angelo
            sewage farm, Tom Green County, Texas .................   25

   6     Daily mean temperatures of air and soil at 10 cm and 40
         cm depths at the San Angelo sewage farm in 1976 .........   26

   7     Water flow rates of three seepage creeks monitored
            on the San Angelo sewage farm ........................   27

   8     Well water sampling locations in Tom Green County, Texas    31

   9     Surface and well water sampling locations at the
            San Angelo sewage farm and Concho River ..............   32

  10     Sampling locations for soil cores taken from the sewage
            farm and for soil cores taken from the control area
            outside the sewage farm (A = Angelo, M = Mereta,
            R = Rioconcho) .................................. .....   84

  11     The effect of sewage effluent irrigation on total
            nitrogen content of the top 200 cm of soil from
            the sewage farm compared to the control area .........   87

  12     The effect of sewage effluent irrigation on exchangeable
            soil ammonium nitrogen content of the top 200 cm
            of soil from the sewage farm compared to the control
            area ... ..............................................   94
                                                               (continued)

                                    vii

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Number                                                                Pagj

  13      The effect of sewage effluent irrigation on exchangeable
               soil nitrate nitrogen content of the top 200 cm of
               soil from the sewage farm compared to the control
               area	   95

  14      The effect of sewage effluent irrigation on exchangeable
               nitrite nitrogen content of the top 200 cm of soil
               from the sewage farm compared to the control area ...   96

  15      The effect of sewage effluent irrigation on total soil
               phosphorus content of the top 200 cm of soil from
               the sewage farm compared to control area	   99

  16      The effect of sewage effluent irrigation on water-soluble
               soil phosphorus content of 200 cm of soil taken from
               the sewage farm compared to the control area 	  100

  17      The effect of sewage effluent irrigation on total soil
               cadmium content of 200 cm of soil taken from the
               sewage farm compared to the control area 	  103

  18      The effect of sewage effluent irrigation on total soil
               chromium content of 200 cm of soil taken from the
               sewage farm compared to the control area	  104

  19      The effect of sewage effluent irrigation on total soil
               copper content of the top 200 cm of soil taken from
               the sewage farm compared to the control area	  106

  20 -     The effect of sewage effluent irrigation on total soil
               lead content of the top 200 cm of soil taken from the
               sewage farm comparted to the control area 	  108

  21      The effect of sewage effluent irrigation on total soil
               nickel content of the top 200 cm of soil taken from
               the sewage farm compared to the control area 	  109

  22      The effect of sewage effluent irrigation on total soil
               zinc content of the top 200 cm of soil taken from
               the sewage farm compared to the control area 	  Ill
                                   viii

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                                   TABLES


Number                                                                Page

   1     San Angelo city Population increase with time 	  10

   2     Cattle present on the San Angelo city sewage farm in 1975 ..  14

   3     Cattle present on the San Angelo city sewage farm in 1976 ..  14

   4     Gross income of San Angelo city sewage farm	  15

   5     Water sampling locations on the San Angelo sewage
            farm and Concho River	  18

   6     Location and depth of water sampling sites outside
            sewage farm	  19

   7     Methods used for chemical analysis of waters 	  21

   8     Equipment and methods used in soil analysis 	  22

   9     Water flow rates of Concho River upstream and downstream
            from the sewage farm in 1976 	  28

  10     Average water flow rates of the Concho River upstream
            and downstream from the sewage farm and of three
            seepage creeks at the sewage farm for 1976 	   29

  11     Chemical anlaysis of raw sewage from Feburary 18, 1976,
            to November 11, 1976, collected from the sewage
            farm treatment plant 	   34

  12     Chemical analysis of water samples from November 18, 1975,
            to November 11, 1976, taken from lagoon no. 1 located on
            the San Angelo sewage farm	   35

  13     Chemical analysis of water samples from November 18, 1975,
            to November 11, 1976, taken from lagoon no. 4 located on
            on the San Angelo sewage farm	   36
  14     Chemical analysis of water samples from November 18, 1975»
            to November 11, 1976, taken from seepage creek no. 1 on
            the San Angelo sewage farm	   37
                                                              (continued)

                                     ix

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Number                                                               Page

  15     Chemical analysis of water samples from November 18, 1975,
            to November 11, 1976, taken from seepage creek no. 2
            located on the Concho River adjacent to the San Angelo
            sewage farm	   38

  16     Chemical analysis of water samples from November 18, 1975,
            to November 11, 1976, taken from seepage creek no. 3
            located on private land on the Concho River adjacent
            to the San Angelo sewage farm	   39

  17     Chemical analysis of water samples from November 18, 1975}
            to November 11, 1976, taken from Concho River sampling
            site no. 1 located upstream from the San Angelo
            sewage farm  	   40

  18     Chemical analysis of water samples from November 18, 1975,
            to November 11, 1976, taken from Concho River sampling
            site no. 2 located downstream from the San Angelo
            sewage farm	;	   41

  19     Chemical analysis of water samples from domestic deep
            wells and shallow ground wells at the sewage farm	   42

  20     Chemical analysis of water samples taken from Tom Green
            County deep wells  	   55

  21     Average COB, BOD,  TFR,  and TNFR analysis  of water
             samples  from the  Concho River,  sewage  farm lagoons,
             seeps  and wells ,  and Tom Green County wells	   59

  22     Recommended surface and irrigation water quality
            criteria for heavy metals	   64

  23     Correlation coefficients and significant probability
            level of daily air and soil temperatures vs.
            concentrations of three seepage creek waters		   67

  24     Correlation coefficients of temperature and nutrient
            concentrations vs. nutrient concentrations of waters
            in the sewage farm  	   69

  25     Nutrient composition and yield for single cuttings of
            NK37 bermudagrass, forage sorghum, rescuegrass and
            Coastal bermudagrass at the San Angelo sewage farm	   ~j\

  26     Yield and nutrient composition of Coastal bermudagrass as
            influenced by N and P fertilization  	   73


                                                             (continued)

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Number                                                               Page

  27     Statistical analysis showing the effect of N and P
            fertilization on plant composition and yields of
            Coastal bermudagrass grown in the sewage farm	  75

  28     Chemical and physical properties of the top 40 cm
            of three soil series from the San Angelo sewage farm ...  77

  29     Statistical analysis of surface soil chemical
            characteristics by F test  	  79

  30     Average concentrations of IN HC1 extractable Cd, Cr,
            Cu, Pb, Ni, and Zn in three soil series from the
            San Angelo sewage farm and the control area  	  81

  31     Partial correlation coefficients and significant
            probability level of chemical characteristics of
            surface soils (0- 20 cm) in the sewage farm and
            the control area  	  83

  32     Distribution of bulk density with depth in three soil
            series from the San Angelo sewage farm and the
            control area	  85

  33     Statistical relationships between soil nutrients as
            related to soil series, treatment, core and depth
            as determined by F test  	  88

  34     Distribution of nutrients with depth in Rioconcho soil
            cores taken from the sewage farm and from an adjacent
            control area  	  89

  35     Distribution of nutrients with depth in Angelo soil cores
            taken from the sewage farm and from an adjacent control
            area  	  90

  36     Distribution of nutrients with depth in Mereta soil cores
            taken from the sewage farm and from an adjacent
            control area	  91

  37     Total nutrient contents of the top 20 cm of soil taken from
            the San Angelo sewage farm and their relative change
            after 18 years of irrigation with sewage effluent
            compared to an adjacent control area 	  92
                                      i.
                                    xi

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                              ACKNOWLEDGMENTS
     The full cooperation of the Environmental Protection Agency was
received throughout the study period.  Field installations and work plans
were thoroughly reviewed by EPA personnel and procedures for chemical
analyses of water, plants , and soil were periodically reviewed to insure
that the methods and quality control would fulfill the requirements of EPA.
Preparation of the report received full guidance and support of the EPA
office at Ada, Oklahoma.  Seventy-six percent of the funding for the
research study was provided by EPA.  The remainder was provided by the
Texas Agricultural Experiment Station.

     This report would not have been possible without the complete support
and cooperation of the city of San Angelo, Texas.  Free access to the farm
and assistance from surrounding landowners was obtained through the city
offices.  We wish to express our appreciation to Mr. Harry Behrend,  farm
manager, and to Mr. Bob Pryor, San Angelo Municipal Sewage Division,  for
their continued assistance and counsel during the study.  Mr. Clarence
Wiedenfeld, soil Scientist with the Soil Conservation Service, assisted in
classification and identification of major soil series on the farm.

     The cooperation of those landowners in Tom Green County who allowed
us to periodically sample their domestic wells is appreciated.
                                     xii

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                                 SECTION 1

                               INTRODUCTION
     Man and his environment must be protected from pollution caused by his
products and wastes.  Disposal of human waste has been one of the most
interesting and persistent problems since the beginning of civilization.
Recently, the practice of land disposal has received much attention as a
modern technique for advanced wastewater treatment.  Public Law 92-500
enacted on October 18, 1972, requires increased treatment of municipal
wastewater to reduce water pollution.  By 1977 all publicly owned wastewater
treatment plants are required to meet the secondary treatment effluent
standard.
                                        /
     The city of San An£p.lo, Texas, has been using the same agricultural
land for disposal of municipal wastewater since 1958.  A primary treatment
involving settling and screening of solids has been used before application
of the sewage wastewater to a 259 hectare farm.  The wastewater has never
been chlorinated or intentionally disinfected.  Much of the applied waste-
water reaches the Concho River, which borders the sewage farm, by underground
seepage.  Direct surface runoff of sewage wastewater from the farm does not
occur because the land has been terraced and borders built to retain the
wastewater.  The soils on the farm are rather shallow clays or clay loams
and have been used for forage and cattle production.  Some hay is removed
from the land and sold.  Much of the land has been planted to Coastal
bermudagrass and is grazed by cattle.

     Land has a great capacity for receiving and decomposing human wastes.
The soil-water-plant system plays an important role in the renovation of
wastewater.  To date, one of the unknown factors in land disposal is the
potential contamination and accumulation of wastes in the field, especially
the long-term effect.  The objectives of this study are as follows:

1.  Determine the fate of applied nitrogen, phosphorus, and selected
    heavy metals in the soil-water-plant ecosystem.

2.  Compare the concentrations of nitrogen, phosphorus, and heavy metals in
    soil, water, and plant samples taken from the sewage farm with those
    taken from adjacent control areas.

3.  Measure the chemical characteristics of sewage farm surface soils and
    evaluate their relationship to the concentrations of nitrogen, phospho-
    rus, and heavy metals in the soils.

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4.  Make recommendations for the disposal of sewage wastewater on the land
    in view of the effect of nitrogen, phosphorus, and heavy metals on the
    environmental quality.

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                                  SECTION 2

                          SUMMARY AND CONCLUSIONS
     The 259 hectare sewage farm had been in operation for 18 years and
received all the sewage from the city of San Angelo at the time this study
was conducted.  San Angelo is located in a semi-arid region of Texas and
receives an average of 53 cm of rainfall per year.  The average annual
temperature is 19.0°C.  The population in 1975 was 67,000.

     Gross yields of forage was much higher than on adjacent areas where
irrigation was not practiced.  Stocking rate of cattle on the sewage farm
was 2.7 head/ha in 1976.  This was twenty times higher than the stocking
rate of surrounding rangeland.  In addition, 21,600 bales of hay were sold.
Total gross income from the farm in 1976 was $57,576.

     Daily flow of sewage to the farm ranged from 0.22 to 0.35 m-Vsec.  The
effluent was separated from solids in a settling tank.  It was then either
pumped directly to the land or passed through one to three storage lagoons
before being distributed to various parts of the farm.  Water from the
lagoons was distributed through a gravity flow underground pipe system where
it was used for irrigation.  Surface irrigation, using bordered terraces, was
used to prevent surface runoff and provide even distribution of water.

     Water moved vertically through the soil until it reached relatively
impermeable soil layers.  The water then moved laterally and surfaced as
seepage creeks or seeps which eventually flowed into the Concho River which
borders the sewage farm.  Seeps along the bank of the Concho River were
observed in the soil profile above the Permian red clay beds.

     The San Angelo sewage farm contributed from 3.7 to 31 percent of the
total flow of the Concho River during 1976.  The average flow contribution
was 0.14 m^/sec (12.5 percent).

     Nitrogen and phosphorus in the effluent were being applied to the farm
in large amounts.  The concentration of N in the water (from lagoon no. 4)
was 29.8 yg N/ml, primarily as NH, and organic N.  The concentration of
phosphorus in the irrigation water was 18.45 yg P/ml and was in the ortho-
or organic phosphate forms.

     Nitrogen present in the seepage waters was in the NO., form.  Mean NO«-N
concentrations in three seepage creeks flowing into the Concho River ranged
from 10 to 21 yg/ml.

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     There was no accumulation of NHt-N or NC-I-N in the soil profiles.  Some
organic N accumulated at the surface of the soil, and there was evidence of
N07-N accumulation in the profiles of the Angelo and Mereta soils.  Oxida-
tion of NH, to NO- must have been very rapid to prevent appearance of NOl-
After 18 years of continuous irrigation with sewage effluent there was a
net loss of total N in the soil profile.

     Phosphorus accumulated in the soil profile.  There was a significant
increase in water soluble phosphorus in the upper 200 cm of soil.  Movement
of phosphorus into well waters and seepage creeks 2 and 3 was 0.06 yg P/ml.
The concentration of phosphorus in seepage creek no. 1, which runs through
the farm, reached a high of 3.80 yg P/ml during the month of June.  The
average concentration of P in seepage creek 1 was 0.79 "jag P/ml.  The average
concentration of P in the Concho River below the sewage farm was 0.078 yg/
ml.

     Application of fertilizer nitrogen and phosphorus to Coastal bermuda-
grass did not increase production.  The test was conducted on a replicated
field plot experiment on the Angelo soil series.  The quantity of nitrogen
and phosphorus in the irrigation water was adequate for maximum production.

     Heavy metal contamination was not a problem on the San Angelo sewage
farm.  There appeared to be some (accumulation of Cd, Cr, Cu, Pb,, and Zn in
the surface horizons of the Mereta soil which was located adjacent to the
effluent lagoons.  There was also a light accumulation of Pb in the surface
of the Angelo soil.  Consistently higher concentrations of Cr throughout
the Rioconcho profile was probably due to natural variation between sample
sites.  In general, the heavy metal concentration of the sewage was low
because of the general lack of industry in the San Angelo area that would
contribute heavy metals.

     Soils nearest to the sewage effluent lagoons have received significantly
more effluent than those located some further distance away.  The total soil
content of phosphorus, nitrogen, and heavy metals were considerably higher
in Mereta and Angelo soils.

     Water from wells located on the sewage farm had a nutrient composition
similar to wells located in Tom Green County.  The average nitrate concen-
tration in domestic deep wells at the sewage farm and surrounding Tom Green
County was 15.7 and 22.0 yg NOl-N/ml, respectively.  The average nitrate
concentration in shallow groundwater wells at the sewage farm was 5.8 yg
NO~-N/ml.  More than 60 percent of the well water samples analyzed had
nitrate concentrations greater than 10 yg NO»-N/ml.  High NCC-N concentra-
tions, exceeding 50 ppm in some wells, should be considered a serious
problem.  The unusually high N0» concentrations appear to be a result of
natural distribution and contamination of the aquifer and not due to the
sewage farm.

     The primary contributions to the Concho River from the San Angelo
sewage farm appear to be an increased flow rate of the river and a slightly
higher NOl-N concentration in the river below the sewage farm.

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                                 SECTION 3

                               RECOMMENDATIONS
     Land disposal of municipal wastewater at San Angelo, Texas, combined
with a forage crop and cattle production system, can be considered a prac-
tical long-term wastewater treatment scheme.  Minimal alteration of the
water quality of surrounding surface streams and' groundwaters has occurred
as a result of the sewage farm operation.  Increased levels of NO--N in the
Concho River appear to be the primary concern and will warrant continued
monitoring.

     Based on the current daily volume of incoming sewage wastewater to the
sewage plant and the present acreage of the sewage farm, it will be neces-
sary in the near future to expand the sewage farm acreage to effectively
dispose of the increasing volume of sewage wastewater.  Disposal of waste-
water over a larger acreage will also eliminate and/or reduce the chances
of direct discharge of sewage wastewaters into surrounding surface streams.
An expanded acreage would'lower the annual loading rate of the soil and
allow more recovery time between applications of sewage effluent.  Lower
annual loading rates of the soil would also extend the useful life of the
farm as a sewage effluent disposal site.

     Nitrogen and phosphorus inputs from sewage effluent are adequate to
meet the nutritional requirements of the crops.  Therefore, additional
applications of these elements as fertilizer are not recommended.  Chemical
and/or biological processes which can reduce the high nitrate concentration
in domestic deep wells at the sewage farm and surrounding Tom Green County
should be considered since these waters are used as a domestic water supply.

     Irrigation with wastewater effluent on the sewage farm for the past
18 years has significantly changed the concentrations of some of the
chemical elements in the soils.  Of those elements determined in this
study, none were present in what would be considered as toxic amounts.
However, as industry expands or the chemical composition of sewage effluent
changes, further investigations and periodic monitoring of the site are
recommended.

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                                SECTION 4

                      GENERAL PROJECT CHARACTERISTICS
 TOPOGRAPHY

     Hilly areas, plains, and river valleys are the three types of  topog-
 raphy present in Tom Green County where San Angelo, Texas is the county
 seat  (Willis, 1954).  Hilly remnants of a northward extension of the
 Edwards  Plateau cover much of the northern, western, and southern parts
 of  the county.  Plains lie east of San Angelo from the northern boundary
 of  the county southward  to the base of the hills of the Edwards Plateau.
 That portion of the plains south of the Concho River is known as Lipan
 Flat.  River valley flats lie along the Concho River and its tributaries—
 the North, Middle, and South Concho Rivers—which join before reaching
 the San  Angelo sewage farm land 8 km east of the city limits on Farm
 Road 380.  The highest point in the county is near the southwest corner,
 located  40 km southwest  of the sewage farm where the altitude is approxi-
 mately 778 m.  The lowest point is the bed of the Concho River at the
 Tom Green-Concho County  line, 19 km down river from the sewage farm where
 the altitude is approximately 503 m.  The location of the San Angelo area
 is  shown in Figure 1.

     The San Angelo sewage farm is located on the plains bordering  the
 north bank of the Concho River.  The land is slightly sloping from  0 to
 3 percent southward toward the river or to a drainage creek in the  northern
 area of  the farm which flows into the Concho River east of the sewage
 farm.

     Adjacent land to the west and north is cleared rangeland, typical of
 the arid region.  The land on the eastern border of the sewage farm is
 cultivated in wheat and irrigated with water pumped from the river.  This
 land gently slopes toward the river and has very productive soil.   The
 private  land on the river surrounded by the sewage farm is similar  to the
 rangeland located west and north of the sewage farm, except for that
 portion  that is productive river valley soil and is cultivated in grain
 sorghum  and wheat.

     The moderate annual rainfall, gently sloping terrain, and properly
managed  grasslands keep erosion in check on the lands adjacent to the
 sewage farm.  Flooding is not a problem due to the three large reservoirs—
Twin Buttes, 0.  C. Fisher, and Lake Nasworthy—which are located on the
river and its main tributaries west of San Angelo.

-------
 Scat'
Source: Adapted from Erwin Raisz, Land forms of the United States, 1957.

Figure  1.  General topography and location of  study  site  at San Angelo,
             Texas.

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     Rangeland adjacent  to the sewage farm has been chained and pushed with
bulldozers  for brush  control and optimum native forage production.  Culti-
vated  land  was root plowed and broken from mechanized row cropping.

     The  land on  the  San Angelo sewage farm, which was initially row
cropped,  has now  been established in permanent pasture except  for three
cultivated  fields which  are used to produce forage sorghum or  small
grains for  grazing and haying.  Due to water disposal requirements which
keep the  soil very wet,  the grass fields are seldom disturbed  by farming
equipment.  The cultivated fields are plowed in early spring and fall for
seedbed preparation prior to planting.
 GEOLOGY

      Rock  formations  exposed in Tom Green County are of sedimentary origin.
 The  oldest of  these belong to the Permian system and are exposed along the
 Concho River  (Barnes  1974).  The regional dip of the Permian rocks is
 westward at a  rate of approximately 9.5 m to the km.  The hilly remnants of
 the  Edwards Plateau are composed of rocks in the Cretaceous system which
 dip  southeastward at  a very low angle.  Older Quaternary alluvium (the
 Leona Formation) covers the Permian rocks in most of the plains area.
 Younger Quaternary alluvium is present in the stream valleys.  A cross
 section of the geologic strata of Tom Green County is presented in Figure 2.

      The land  in the  sewage farm area is located on the Leona formation
 which is typified by  the conglomerate along the Concho River.  The conglo-
 merate is  classified  as Pleistocene and Pleistocene alluvium and is derived
 from rocks of  the Edwards Plateau.  The alluvium was deposited on the
 eroded surfaces of the Permian rocks over about 1036 square km in the
 plains areas of the county.  The thickness of the alluvium ranges from
 1 m  to about 38 m.  The alluvium is composed of discontinuous beds of
 poorly sorted, rounded to subangular gravel, conglomerate, sand, silty
 clay, and  caliche.  The upper 20 m of soil is predominately clay and clay
 loam.  The particles  of gravel are composed of limestone and flint, some
 of which contain fragments of Cretaceous fossils.  Rocks in the county
 which are  older than  those exposed are not known to contain potable water.
 Water wells in the county do not penetrate these older rocks.  Most of
 the  irrigation wells  in the county draw water from the more permeable
 layers of  gravel and  creviced conglomerate.
GROUNDWATER

     Groundwater in the area is derived strictly from precipitation on the
land surface, except for the immediate sewage farm area and the Lipan
Flat where irrigation has contributed to water tables.  The non-precipita-
tion contribution to groundwater in the sewage farm area is the municipal
sewage effluent from the city of San Angelo which is used for irrigation
and, in the Lipan flat, the water from the irrigation canal from Lake
Nasworthy-

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VO
                                          SOUTH CONCHO RIVER
               CRETACEOUS, UNDIFFERENTIATED
                                                                   STANDPIPE LIMESTONE MEMBER
                                                         10
                                                        _J
                                                                                                                         400
                                                                                                                      METERS
                                         KILOMETERS
                      Figure 2.   Cross section of the geologic strata of Tom Green County, Texas

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      The Texas Board of Water  Engineers  in  cooperation with the  U.  S.
 Geological Survey have found that  most water wells  in the  area are  in  the
 Leona formation and  range  to about 30.5  m in depth.
 CITY POPULATION AND  INDUSTRIAL  INPUT

      The city of San Angelo  is  a  steadily growing  community with  a  1975
 population estimated at  67,000.-  Daily  sewage rates  to  the treatment
 plant are from 0.219 to  0.241 m /sec with a minimum  flow of 0.153-0.175
 and a maximum flow of  0.307  to  0.351 m  /sec.  Sewage inputs are
 dominately domestic.   Industrial  flow  in  1977 accounted  for  0.0162 m /sec
 according to  the  city water department.   Major  industries  involve a
 creamery with the greatest input, 4 packing plants, 2 large  commercial
 laundries,  1  bottling plant, and  2 foundaries which contribute recycled
 cooling water.

      Population growth of the  city of  San Angelo from 1910 to 1975 is
 listed in Table 1.

           TABLE 1.   SAN ANGELO CITY POPULATION  INCREASE  WITH TIME


                    Year                    Population
1910
1920
1930
1940
1950
1960
1970
1975a
10,321
10,050
25,308
25,802
52,093
58,815
63,884
67,000
 o
 estimated
WASTEWATER TREATMENT FACILITIES

     The sewage treatment plant of the city of San Angelo, Texas was designed
for primary treatment by screening, sedimentation, skimming, and separate
digestion of sludge.  Digested sludge was to be mixed into settled sewage
or placed on a designated area and plowed into soil by mechanized farm
equipment.  During this project a new sewage treatment plant was
under construction and the original method of handling had to be altered.
The method used during the time this project was underway was simply primary
treatments of sewage and piping both solids and liquids to lagoon .1, lagoon
2, or occasionally flooding land directly with the primary treated sewage.
                                                        3
     Presently the sewage plant handles 0.219 to 0.263 m /sec of incoming
sewage.  The sewage enters the plant by a 0.76 m diameter line.  After


                                     10

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passing through a bar screen the sewage was lifted to a clarifier  (18.3 m
in diameter x 0.61 m deep) by alternating 0.372 and 0.456 nrVsec electric
pumps.  Sometimes sediment from the clarifier was pumped into an adjacent
6.19^ha surface area sludge lagoon, and sewage effluent was pumped from the
clarifier to the first of a series of three lagoons located at the opposite
end of the farm.  Other times both effluent and sludge were pumped into
lagoon 1 or lagoon 2.  The total surface area of the three lagoons was 17.68
ha.  In these three lagoons the sewage was subjected to oxidation and further
settling.  Effluent overflowed from the second lagoon to the third lagoon
and from the third lagoon to the fourth lagoon.  Water for irrigation was
used directly from each lagoon.

     Irrigation was accomplished by distribution from underground mains into
level borders of approximately 0.2 ha each.  The borders were separated by
levees not less than 0.25 m high, and each field had a perimeter levee on
its lower side, built to a minimum height of 0.46 m, to insure that sewage
effluent did not flow directly from the field to a water course.  Specifica-
tions for the three types of levees present on the farm are shown in Figure
3.
VEGETATION ON FARM

     There were approximately 259 ha of the sewage farm that received sewage
effluent and were used for hay production and grazing.  Coastal bermudagrass,
Kentucky 31 fescue, and NK 37 bermudagrass were the forage grasses in the
permanent pastures.  There were approximately 127 ha of Coastal bermudagrass
(Cynodon dactylon).  The growing season at the sewage farm for Coastal ber-
mudagrass is from March through September.  Kentucky 31 fescue (cool season
perennial) grew from November through June and there were approximately 40
ha of the farm in this grass.  NK 37 is a hybrid bermudagrass variety of the
Northrup King seed company.  It is a warm season bermudagrass.  There were
approximately 77 ha of cultivated land on the sewage farm.  A warm season
annual forage sorghum was utilized for hay and grazing in the spring and
summer.  Following the last harvest of the forage sorghum in the early fall
the cultivated land was plowed and planted in oats.  The oats receive strong
competition from rescuegrass (Bromus wildenowii), a cool season annual
native, which was very prolific.  Rescuegrass could be found growing any-
where on the farm by late winter, and if the bermudagrass sod was plowed in
the fall, an excellent stand of rescuegrass occurred.

     All the grasses on the sewage farm were utilized for hay.  Hay fields
were cut frequently and provided a primary source of income for the farm.
Frequency of cutting depended on weather limitations, grazing intensities,
and flooding frequency as dictated by sewage accumulations.  In 1975 ap-
proximately 27,800 bales of hay were sold despite the yearly average grasing
intensity of 2.08 head of cattle per ha.  In 1976 approximately 21,600 bales
of hay were sold during a grazing intensity of 2.69 head of cattle per ha.
For native range the 508 mm rainfall average restricted stocking rates to
one animal unit per 8 ha on good cleared range and to one animal unit per
20 ha on poor brushy range.
                                     11

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                              1.22m
                                  1.83m
 compacted
 earth  fill
                              8.53m
               Typical section of holding pond levee
                              1.22m


                          Border levee
                                                  .25m (minimum)
                              2.44 m
                        Field perimeter levee
                                               t«
.46m (minimum)
Figure  3.  Cross sections of three different types  of levees present on
                     the San Angelo  sewage farm.
                               12

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     Natural vegetation in the area is greatly dependent on grazing
practices.  Although very little native vegetation occurred on the farm,
other than weeds such as pigweed (Atnaranthus retroflexus), nightshade
(Solanum sp.)> or mesquite trees (Prosopis sp.)> the immediate area sur-
rounding the farm is inhabited by grasses, brush, and short trees.  The
predominant warm season grasses are sideoats grama (Bouteloua cutipendula),
cane bluestem (Andropogon barbinodis), buffalo grass (Buchloe dactyloides),
vine mesquite (Panicum obtusum), plains bristlegrass (Stefai leucopila),
purple threeawn (Aristida purpurea), and other arid area species.  The
predominate cool season grasses are rescue grass (Bromus wildenosii),
Canada wildrye (Elymus canadensis), and Texas wintergrass (Stopa leuotricha)
Trees in the area are predominately mesquite (Prosopis juliflora) and are
seldom over 6 meters tall.
ANIMAL POPULATION AND CROP PRODUCTION

     Cattle are the only livestock grazed on the sewage farm.  All animals
are rotated from field to field to permit haying, flooding, planting^ or
any other operation and are not grazed specifically in any one field or
combination of fields.  All classes of cattle are grazed on the farm and
grazing records are kept according to animal grazing days (AGO).  One
animal grazing day is defined as the presence of one cow, cow and calf,
or a bull grazing on the sewage farm for one day.  Yearling cattle are
listed as cows in the farm records since they consume approximately the
same amount of forage.

     Approximately 259 ha of the farm are flood irrigated with sewage
effluent, grazed, and hayed.  The data in Tables 2 and 3 show livestock
numbers on the farm for 1976 and 1976.  The average number of cattle per
month and the livestock grazing intensity (head/ha) are also shown.

     Since 1976 was a wet year, it was not a good year for hay production
because cut hay was rained on, and rainfall interrupted other forms of
haying.  The sewage farm may realize more income by intensifying haying
operations and decreasing livestock numbers, especially during the warm
season of the year when hay production is greatest.  The gross income of
sewage farm in 1975 and 1976 are shown in Table 4.
                                      13

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Animal Grazing Days
Month
Jan.
Feb.
Mar.
Apr.
May
June
July
Aug.
Sept.
Oct.
Nov.
Dec.
Cows
7863
6120
7452
7500
12054
21146
19170
21644
20640
17874
17640
13524
Pairs
1830
1830
1830
1830
1740
1740
1740
1740
1740
1140
1140
780
Bulls
367
330
330
330
180
180
120
150
150
150
150
120
Total
10060
8280
9612
9660
13974
23066
21030
23534
22530
19164
18930
14424
Head per
Month
335
276
320
322
466
769
701
784
751
639
631
481
                        Total 6475
Average head per month (1975)  540
  Grazing intensity (head/ha) 2.08
TABLE 3. CATTLE PRESENT ON THE
SAN ANGELO CITY SEWAGE FARM IN 1976
Animal Grazing Days
Month
Jan.
Feb.
Mar.
Apr.
May
June
July
Aug.
Sept ,
Oct.
Nov.
Dec.
Cows
9210
12330
17220
34287
23640
23640
23640
22500
11570
21870
20566
15135
Pairs
780
780
780
780
780
780
780
780
780
1050
1050
300
Bulls
120
210
210
240
180
180
180
180
180
180
150
150
Total
10110
13320
18210
35307
24600
24600
24600
23460
16530
23100
21766
15585
Head Per
Month
337
444
607
1177
820
820
820
782
551
770
726
520
                         Total 8374
 Average head per month (1976)  698
   Grazing intensity (head/ha) 2.69
14

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TABLE 4-  GROSS INCOME OF SAN ANGELO CITY SEWAGE FARM
Year
Item
Unit Price
Income

1975


1976


27,828 bales
294,264 AGD

21,632 bales
251,188 AGD
$
1.50
0.10

1.50
0.10
$
41,742.00
29,426.40
Total 71,168.70
32,448.00
25,118.80
                                                   Total  57,567.30
   AGD  is  animal  grazing  days.
                                      15

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                                 SECTION 5

                           METHODS AND MATERIALS
HYDROLOGY AND METEOROLOGY

     Water flow of three seepage creeks in the sewage farm was continuously
monitored over a one year period by installation of weirs in conjunction
with a water level recording device (Stevens type F recorder, model 68).
A 90-degree V-notch weir was installed in seepage creek 1.  A 60-degree
V-notch was installed in seepage creeks 2 and 3.  This weir design permitted
accurate measurement over a wide range of water flow.  Measurement of
water flow of the Concho River was determined by the area-velocity method
with a F583 Pygmy-type water current meter on the Concho River at
sampling sites above and below the sewage farm.  These measurements pro-
vided an estimate of water contribution of the sewage farm to the Concho
River.  Irrigation flow of sewage' effluent to the fenced Coastal bermudar
grass area was determined with a commercial type Badger magnetic drive
irrigation meter.  The location of weirs, Concho River sample sitesy, and
the fenced plot area in relation to the effluent lagoons are shown in
Figure 4.

     A temperature recording device (Weathermeasure Corp. model T603, three
point thermograph) was installed in the fenced Coastal bermuda/grass area.
Air temperature and soil temperatures at 10 cm and 40 cm depths were
monitored hourly.  Precipitation was recorded at the fenced plot area with
a weighing rain gauge made by Belfort Instrument Company.
WATER ANALYSIS

Water Sampling Locations

Sewage Farm Locations—
     The on-farm water sampling sites were located across the  entire  area.
These locations were selected to permit monitoring of sewage effluent from
the time it entered the treatment plant until it flowed into the Concho
River adjacent to the sewage farm through seepage creeks.  Ground water
was sampled from shallow (1.5-9.1 m) wells and from deep (21.3-36.6 m)
wells located within the sewage farm boundary (Table 5).

Non Sewage Farm Locations—
     Water samples were collected from domestic wells in Tom Green County,
Texas on an irregular basis.  These sampling sites are located within a
                                     16

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                     Shallow grou
                      water wells
O
O
O
O
  •=}
 Fenced I
plot area
                                                 Weir
                        Pond
                   Seepage creek
                        No. I
     Sewage/
effluent lagoons
                    Seep No. 3
                      weir
             Figure 4.  San Angelo sewage farm with blow up
                of Coastal bermudagrass  fenced plot area.
                                  17

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                     TABLE 5.  WATER SAMPLING LOCATIONS
               ON THE SAN ANGELO SEWAGE FARM AND CONCHO RIVER
Sampling
 Number                               Location
    1         Upstream Concho River:  on the west bank of the river 0.64
              km south of the bridge over the river on farm road 380

    2         Downstream Concho River:  0.84 km down the river from the
              sewage farm

    3         Lagoon #1:  on the east side of lagoon directly west of the
              old clarifier

    4         Lagoon #4:  on the east side of lagoon where the water over-
              flows from the lagoon into the irrigation mains

    5         Seepage  creek #1:  the large seepage creek on the eastern
              border of the farm.  Sample was taken from the pond created by
              the weir and on the south side 30 feet upstream from the weir

    6         Seepage  creek #2:  The spring as it flows from the river bank
              into a pond created by the river weir

    7         Seepage  creek #3:  The spring that flows from the river bank
              on Mika private land on the north side of the river 2.01
              km down river from sampling site #1

    8         Shallow well (2.1 m) on the south side of the seepage creek
              #1 on the bank just above the creek about 180 meters above
              the weir at sampling site #5

    9         Deep well (29.0 m) at the sewage treatment plant

   10         Deep well £30.5 m) at the hay barn

   11         Deep well (33.6 m) on the west edge of the Carson Farm on the
              east border of the city farm

   12         Deep well (36.6 m) at the cattle pens just north of the sludge
              lagoon

   13         Deep well (27.5 m) at the southeast corner of lagoon #4

   14         Deep  well (21.4 m) at the earth dammed pond approximately
              0.8 km

   16        Deep  well (30.5 m) at the house 137 m west of sampling site
             # 11
                                                               (continued)

                                     18

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TABLE  5i (continued)
Sampling
Number
17

18

19

20

21

22
TABLE 6.
Sampling
JQ umber

31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48

Location
Shallow well (1.2 m) on the north side of seepage creek #1
36.6 m above sampling site #5
Shallow well (7.6 m) at the top of the Coastal bermudagrass
field in the northeast corner of the sewage farm
Shallow well (6.3 m) four borders below sampling site #18
and in the same field.
Shallow well (7.8 m) five borders below sampling site #19
and in the same field
Shallow well (9.3 m) six borders below sampling site #20 and
in the same field
The incoming sewage effluent at the sewage treatment plant
LOCATION AND DEPTH OF WATER SAMPLING SITES OUTSIDE SEWAGE FARM
Distance and direction
Location from sewage farm well depth
Air km m
Veribest NE 6.4 18
Veribest NE 5.3 24
Veribest SE 6.1 46
Veribest SW 1.6 61
San Angelo N 2.1 37
San Angelo N 2.4 37
Red Creek N 3.2 28
San Angelo NW 10.1 34
San Angelo SW 9.7 18
San Angelo SW 9.7 29
San Angelo SW 7.2 20
Wall S , 12.6 42
Wall SE 15.3 41
Eula SE 8.8 46
San Angelo S 4.8 64
Wall SE 14.5 41
Eula SE 11.3 43
Veribest E 5,6 43
                                       19

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16 km radius of the sewage farm.  The samples were analyzed and used
as background information to compare with that of the sewage farm (Table 6).

Chemical Analysis

     Beginning November 18, 1975 waters in the sewage farm were sampled
regularly over a one year period for chemical analysis.  Samples were
taken initially on a weekly schedule and later on a biweekly schedule.
Collecting, handling, and analysis of water samples conformed to the general
procedures outlined by the U. S. Environmental Protection Agency (197?,
1973, 1974).  Analytical procedures used for analysis of water samples are
presented in Table 7.

     Temperature and pH values were determined on site at the sewage farm.
Ammonia-N, nitrate-N, nitrite-N, total Kjeldahl-N, total phosphorus, dis-
solved orthophosphate, and total heavy metals (Cd, Cr, Cu, Ni, Pb, and Zn)
were determined at the College Station laboratory.  Forms of nitrogen and
phosphorus were determined within 24 hours of collection.  Water samples
were analyzed on two different dates for total filterable residue, total
nonfilterable residue, biochemical oxygen demand, and chemical oxygen
demand.
SOIL ANALYSIS

     Three major soil series (Angelo, Mereta, and Rioconcho) were identified
in the San Angelo sewage farm.  A total of 15 soil cores were taken from
each major soil series with a stainless steel auger (available from Arts
Machine Shop, American Falls, Idaho); 10 cores in the sewage farm and 5
cores in an adjacent control area.  Soils were taken to a depth of 200 cm,
except where a gravel or hard caliche layer existed in the soil profile.
The control areas have never been irrigated with sewage effluent.  Each core
was subdivided into 10 subsamples in varying depth increments.  A subsample
of each soil was stored in a freezer under field moisture conditions and
used immediately for chemical analysis of NH.-N, NO~-N, and NOl-N.  The rest
of the soil was air-dried and ground to pass a 1-mm sieve (2-mm for texture
analysis) using a mortar and pestle (Table 8).
FIELD PLOT EXPERIMENT AND PLANT ANALYSIS
                                                     2
     Twenty-seven experimental plots (1.830 x 4.575 m ) were established in
a fenced Coastal bermudagrass plot area in the sewage farm.  The area was
fenced to exclude animal movement into the plots but not to exclude
irrigation with sewage effluent.  These plots were used for biological and
chemical tests where recycling of organisms and nutrients through animals
was to be avoided.

     A factorial experiment (3x3x3) where combinations of three rates
of nitrogen (0, 112.2, and 224.4 kg N/ha as NH.NOJ and phosphorus (0, 56.1,
and 112.2 kg P_05/ha as 0-20-0) and three replications was designed.  These
plots were completely randomized in the field.  Forage was clipped from the


                                     20

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          TABLE 7.   METHODS USED FOR CHEMICAL ANALYSIS OF WATERS
Measurement
1.
2.
3.
4.
5.
Temperature
pH
NH. -N
4
NOg-N
NO;-N
Method
Thermometer
Corning model 7 pH meter
Distillation with MgO and
collected in HoB03
Brucine sulfate
Diazotation of sulfanilamide
Reference
EPA 1974
EPA 1974
Bremner 1965 b
EPA 1974
EPA 1974
6.  Total Kjeldahl
    nitrogen
Hot, concentrated
digestion and distillation
with NaOH
  EPA 1974
7.  Total
    Phosphorus
8.  Dissolved
    orthophosphate

9.  Heavy metals
HNQ3-HC104 digestion and
antimony-phospho-molybdate
complexation

Antimony-phospho-molybdate
complexation

HNO. digestion and HC1
dissolution
  APHA 1976
Murphy & Riley 1962
  EPA 1974
Murphy & Riley 1962

  EPA 1974
10. COD
11. BOD
12. Total
    filterable
    residue

13. Total
    nonfilterable
    residue
Digestion with potassium
dichromate and back titration
with ferrous ammonium sulfate

Incubation for 5 days at 20°C
followed by 02 analysis with
Yellow Springs model 57
dissolved oxygen meter

Filtering through 0.45 V-
millipore filter paper and
dried at 180°C

Filtering through 0.45 U
millipore filter paper and
dried at 105°C
  APHA 1976
  APHA 1976
  EPA 1974
  EPA 1974
                                      21

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           TABLE 8.  EQUIPMENT AND METHODS USED IN SOIL ANALYSIS
Measurement
            Method
                                                               Reference
1.
2.
3.

4.

Texture
Bulk density
pH

Carbonate

Bouyoucous hydrometer
Core method
Metrohm/Brinkmann model
pH-103 pH meter
Acid-neutralization

Day 1965
Blake 1965
Peech 1965

Allison and
Moodie 1965
5.  Organic matter

6.  Cation exchange
    capacity

7.  Soluble salts
8.  Total phosphorus
9.  Water soluble
    Phosphorus
10.  Total nitrogen

11.  NO;-N
12. NHj-N
13. N02-N
14. acid-extractable
    heavy metals
15.  Total heavy
     metals
Walkley-Black method

pH 7.0 IN  NH^OAc extraction
and Kjeldahl distillation

Industrial Instruments Inc.
Model RC 16B2 conductivity
bridge
       digestion and vanadate
molybdate color method
1:10 soil-water extraction
and ascorbic acid method
                                     Jackson 1958

                                     Keeney and
                                     Bremner 1969

                                     Bower and
                                     Wilcox 1965
                                     Kao and Blancher
                                     1975, Tandon,
                                     et al, 1968

                                     Olsen and Dean
                                     1965, Murphy
                                     and Riley 1962
                        Kjeldahl digestion and distillation  Bremner 1965 a.
                                     Bremner 1965 b.
                                     EPA 1974

                                     Bremner 1965 b.
1:10 soil-2N KC1 extraction
and brucine sulfate color method

1:10 soil 2N KC1 extraction
and kjeldahl distillation
with MgO

1:10 soil-2N KC1 extraction
and diazotation of sulfanilamide

1:2 soil-lN HC1 extraction and
determination by using a Perkin-
Elmer Model 403 atomic adsorption
spectrophotometer

HNO-j-HClO, digestion and determina-  Kao and Blanchar
tion using a Perkin-Elmer model 403        1973
atomic ab'sorption spectrophotometer	   	
              22
                                     Bremner 1965 b.
                                     EPA 1974

                                     Lagerwerff 1971

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experimental plots twice during the active growing season.  Yield of
forage grass was calculated from the plot weights.  Yield of major grass
crops in other areas of the sewage farm was also determined.  Once dry
matter yield was measured, the plant tissue was used for chemical analysis.
Plant tissue was dried at 75 C for 24 hours and ground with a T-Jiley Mill to
pass through a 40 mesh sieve prior to analysis.

     Total phosphorus was determined by the procedure reported by Kao and
Blanchar (1973) and Tandon et al.  (1968).  The semimicro Kjeldahl method
was used to determine the total nitrogen concentration (Bremner, 1965a).
Heavy metals were determined by digesting a suitable amount of plant tissue
in hot, concentrated HNO, and HC10, in a micro-Kjeldahl flask (Perkin-
Elmer Corp. 1971) and analyzed with a Perkin-Elmer Model 403 Atomic
Adsorption Spectrophotometer.
 STATISTICAL ANALYSIS

     All  field  and  laboratory  data were  processed and analyzed by the
 Statistical Analysis  System (Barr et  al.,  1976)  on an IBM 370 computer at
 Texas A & M University.
                                      23

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                                  SECTION  6

                           RESULTS AND DISCUSSION
HYDROLOGY AND METEROLOGY

Meteorology

      The climate of San Angelo, Texas is semiarid to subhumid.  The average
annual precipitation is 52.78 cm with about 85 percent of the precipitation
falling between mid-March and mid-November.  On the average, rain or snow
falls during all or parts of 42 days a year.  Freezing temperatures occur
at  intervals between mid-November and mid-March (Willis 1954).  The data
presented in Figure 5 show average monthly precipitation from 1936 to
1975  and monthly precipitation in 1976 at the sewage farm.  The precipitation
was 64.29 cm in 1976, about 22 percent higher than the average of the last
40  years.

      Temperatures of air, soil at 10 cm depth, and soil at 40 cm depth are
shown in Figure 6.  Soil temperatures fluctuated less than the air
temperature.  There was little difference between the soil temperature at
10  cm and at 40 cm.  Soil temperatures at 40 cm ranged from a high of 30 C
to  a  low of +5 C.  Air temperatures fluctuated between a high of 31 C to a
low of -1 C.  Maximum soil temperatures at 10 and 40 cm depths are about
50  days behind the maximum air temperature.  Both the soil temperature at
10  cm depth and soil temperature at 40 cm depth show the same pattern.

Hydrology

      Water flow rates of the Concho River measured on 32 separate days in
1976  upstream and downstream from the sewage farm are shown in Table 9.  The
mean  water flow rates upstream and downstream from the sewage farm were
U0007 and 1.1436 m /sec.  This indicates a net contribution of 0.1429
m /sec from the sewage farm to the Concho River.  It was calculated that, on
the average, the San Angelo sewage farm contributed about 12.5 percent of the
total water flow of the Concho River downstream from the sewage farm in
1976  (Table 10).  Data presented in Figure 7 show the water flow rates
of  the three seepage creeks monitored on the sewage farm.  Seepage creek
no. 1 had a daily mean flow rate of 1,009.5 liters per minute.  Flow rates
ranged from 53.0 to 3,225.1 liters per minute with a daily mean standard
deviation of 465.9 liters per minute.  Seepage creek no. 2 had a daily
mean  flow rate of 166.6 liters per minute and ranged from 2.5 to 550.0
liters per minute with a daily mean standard deviation of 33.5 liters per
minute.   The mean flow rate of seepage creek no. 3 was 97.6 liters per
minute with a range of 17.9 to 525.0 liters per minute and a daily mean

                                      24

-------
    20"
                        1976


                        avg. 1936-1975
E
u

Z
o
Q.


O

UJ

OC

Q.
15"
     5  •
             Jan  Feb  Mar  Apr  May  Jun  Jul  Aug  Sept Oct   Nov  Dec



                                       MONTH
 Figure 5,  Average monthly precipitation for the years 1936  - 1975 and monthly precipitation

               in 1976 at the San Angelo sewage  farm,  Tom Green County,  Texas.

-------
             30
             25
             20-
             15-
             10"
             5"
             30-
            25- •
            20-•
TEMPERATURE   15
    "C

             10- •
             S'-
            30-
            25-
            20-
             15-
             10"
             5"
                   AMBIENT
                  SOIL- 10 cm
                  SOIL -40 cm
             0-1	1	1	1	1	1-
                JAN   FEB   MAR   APR   MAY   JUN   JUL   AU6   SEP  OCT   NOV   DEC

                                            DATE

               Figure 6.   Daily mean temperatures  of air,
                   soil  at 10 cm and  40 cm  depths at
                    San  Angelo  sewage farm  in 1976.
                                     26

-------
         700-
         600' •
         500"
         400"
         300--
         200
          100-
           0

          90


          80


          70


          60


FLOW RATE   50
  GPM
          40 •


          30


          20


          10
              SEEPAGE CREEK NO. I
SEEPASE CREEK NO. 2
                        -I	1-
                                      H	H-
          80"
          70-
          so-
          50"
          40-
          30-
          20--
          10-
              SEEPAGE CREEK NO. 3
                                                              -H
            NOV  DEC   JAN   FEB  MAR   APR   MAY   JUN  JUL  AUS   SEP   OCT

                                      DATE
   Figure  7.  Water flow rates  of  three seepage creeks
         monitored  on  the San Angelo sewage farm.

                                   27

-------
TABLE 9.  WATER FLOW RATES OF CONCHO RIVER UPSTREAM AND DOWNSTREAM
                  FROM THE SEWAGE FARM IN 1976
Water flow rate
Date
760129
760205
760212
760219
760226
760304
760311
760316
760325
760401
760408
760415
760422
760430
760511
760527
760610
760623
760708
760719
760728
760813
760830
760908
760922
761001
761014
761022
761025
761103
761124
mean
Downstream

1.2899
0.7399
0.8899
1.0599
0.5900
0.7799
0.8999
0.8500
0.8059
0.6920
0.9570
1.1419
0.9590
2.3429
1.2659
0.9789
0.4910
0.3750
0.7500
2.1858
1.4569
0.7759
1.7859
1.5500
1.1930
0.8580
1.1790
1.2739
1.4619
1.6669
1.3289
2.0229
1.1436
Upstream
- m /sec —
1.2299
0.6700
0.7199
0.9499
0.5300
0.6300
0.7599
0.6999
0.7629
0.5870
0.8980
0.9699
0.8770
2.1548
0.9759
0.8170
0.3380
0.3510
0.6159
1.8399
1.1609
0.6669
1.5539
1.4901
0.9961
0.7520
1.0361
1.2159
1.1369
1.5079
1.1739
1.9548
1 . 0007
Difference

0.0600
0.0699
0.1700
0.1100
0.0600
0.1499
0.1400
0.1501
0.0430
0.1050
0.0590
0.1720
0.0820
0.1881
0.2900
0.1619
0.1530
0.0240
0.1341
0.3459
0.2960
0.1090
0.2320
0.0599
0.1969
0.1060
0.1429
0.0580
0.3250
0.1590
0.1550
0.0681
0.1429
Difference , ni
T. X 10'
Downstream
4.65
9.46
19.11
10.38
10.17
19.23
15.56
17.65
5.33
15.17
6.16
15.06
8.55
8.02
22.91
16.54
31.16
6.41
17.87
15.83
20.31
14.05
12.99
3.87
16.51
12.35
12.13
4.55
22.23
9.54
11.66
3.36
12.50
                               28

-------
TABLE 10.  AVERAGE WATER FLOW RATES OF THE CONCHO RIVER
         UPSTREAM AND DOWNSTREAM FROM THE SEWAGE FARM
    AND OF THREE SEEPAGE CREEKS AT THE SEWAGE FARM FOR 1976
Location

Concho River upstream
from sewage farm
Concho River downstream
from sewage farm
A
Sewage farm output
Seepage creek no. 1
Seepage creek no. 2
Seepage creek no. 3
Sum of three seepage creeks
Average percentage contribution
to sewage farm output.
Average percentage contribution
to downstream Concho River.

GPM
15861.0
18127.0

2266.0
266.7
44.0
25.7
336.4
of three
of sewage
Flow rate
MGD
22.840
26.103

3.263
0.384
0.063
0.037
0.484
seepage creeks
farm output

m /sec
1.0007
1.1436

0.1429
0.0168
0.0028
0.0016
0.0212
14.85%
12.50%
 *Difference from upstream to downstream
                                29

-------
 standard  deviation  of  24.1  liters  per  minute.   It  was  calculated that
 these  three  seepage creeks  contributed about  14.85 percent of the water
 flowing into the  Concho  River  from the sewage  farm (Table 10).   Approximately
 100 active seeps  have  been  observed discharging water  to the Concho River
 (Texas Water Quality Board  1973).

     The  wastewater that is being  constantly applied to  the sewage farm is
 apparently percolating to the  shallow  bedrock  layer and  moving  laterally
 to the Concho River.   Discharge measurements made  on October 24,  1973 in
 the Concho River  above and  below the sewage farm by Texas Water Quality-
 Board  indicated that on  that date  0.2747 m /sec entered  the river.   This
 volume made  up approximately 43 percent of the flow in the river downstream
 from the  sewage farm.  The  highest percentage  contribution of water from
 the sewage farm to  the Concho  River measured in 1976 was 31.2 percent on
 June 10,  whereas  the lowest value  was  3.4 on December  10 (Table 9).

     The  Concho River  originates in central Tom Green  County at the
 confluence of the north  and south  forks and flows  through Tom Green and
 Concho Counties in  an  eastward direction to its mouth  with the  Colorado
 River, a  distance of approximately 85  km.  The city of San Angelo's  sewage
 treatment plant has a  permitted discharge volume of 0.2234 m /sec (Texas
 Water  Quality Board 1973).  The discharge of treated sewage effluent from
 the city  of  San Angelo's sewage treatment plant is  significantly  increasing
 the flow  of  the Concho River.  At  the  present  time  a new activated  sludge
 wastewater treatment plant  that will discharge an average of 0.3198  m /sec
 of treated domestic sewage  to  the  farm is being constructed.
 WATER ANALYSIS

      One  of  the purposes of this portion of the study was  to evaluate  the
 environmental impact of the city of San Angelo's sewage treatment plant
 discharge on the water quality of the receiving Concho River.  At the
 present time no absolute water quality criteria values are available.
 Water quality criteria, published by U. S. Environmental Protection Agency
 in  1976,  were based on the concentration-effect relationship of a partic-
 ular  water quality constituent upon a particular aquatic species within
 the limits of experimental investigation.  However, those  water quality
 criteria  published by the U. S. Environmental Protection Agency (EPA 1976)
 will  be used to evaluate the waters in the sewage farm and in Tom Green
 County.   As  stated in that publication, those water quality criteria levels
 should provide for the protection and propagation of fish  and othe'r aquatic
 life  and  for recreation in and on water in accord with the 1983 goals  of
 Public Law 92-500.  Water quality criteria published by the National
 Technical Advisory Committee (NTAC) to the secretary of the Interior
 (NTAC, 1968) will also be used.  Furthermore, water quality criteria from
 states having the most stringent standards will be also used as a reference
 (Lykins and Smith, 1976).

     Water sampling locations at the sewage farm and in Tom Green County
are shown in Figures 8 and 9.
                                      30

-------
           SAN ANGELO CITY
                                                                   To Eden
Figure 8.  Well water  sampling locations in Tom Green County, Texas.

-------
 To Son
                                                                RocK
Figure 9.   Surface and well water sampling locations  at  the San Angelo
                      sewage farm and Concho River.
                                 32

-------
     Data presented in Tables  11  through 18  summarize water  analyses
of the incoming sewage effluent,  effluent lagoons  1 and 4, seepage  creeks
1, 2 and 3, and the concentration of nutrients  in  the Concho River  above
and below the sewage farm.  The chemical composition of water  from  shallow
and domestic wells on the sewage  farm is summarized in Table 19.  Eighteen
domestic wells were monitored  on  an irregular basis outside  the perimeter
of the sewage farm during 1976.   These  data  are presented in Table  20.
Finally, biological oxygen  demand (BOD)  and  chemical oxygen  demand  (COD)
are presented for all sampling sites in Table 21.
      \
Temperature

     Solution temperatures  varied with  the time of year of sampling.  Sample
temperatures were as low as 3.8°C on January 3,  1976 at lagoon number 1
and as high as 32.6°C on July  7,  1976 at lagoon no. 4.  Temperature
fluctuations of water from  seepage creeks 2  and 3  were very  small, ranging
from 18.9 to 21.5°C for  the duration of the  study  (Tables 15 and  16).
     The discharge of water  from  the  sewage  farm  into the Concho River had
no effect on the pH values of  the Concho River below the sewage farm
(Tables 17 and  18).  A r:s £.11 increase in pH  value and ammonia-nitrogen
concentration was observed in  the incoming sewage effluent at the sewage
treatment plant and lagoon no.  4.  Ammonification of organic nitrogen and
dissolution of  ammonia in wastewater  increase both  the pH and ammonia-
nitrogen in wastewater.  All three seepage creeks,  shallow ground wells,
domestic deep wells at the sewage farm, and  Tom Green County deep wells had
pH values of 7.2 ± 0.2.

Nitrogen

     Ammonium (Nilt) , nitrate (N0~) , nitrite  (N0~) ,  and organic nitrogen^
are the most important forms of nitrogen in  the nitrogen cycle.  The NH^
ion is either released from  proteinaceous organic matter and urea (Ammonifi
cation) , or is  synthesized in  industrial processes  involving atmospheric
nitrogen fixation.  The NOl  ion is formed by the  biochemical oxidation of
NH, to N0~ (nitrification);  NOg is an intermediate  produc£ of the
nitrification process.  The  NOl ion is formed from  the NH^ or the NO- ions
by certain biochemical processes  (nitrification) .   In an aerobic condition
N0~ is rapidly  oxidized to N0~.   Plants can  take  up both NH^ and N0~ ions
and convert them, to protein.   When nitrogen  in sewage effluent whicfi is
mostly in the NH, form is applied to  an aerobic soil, the NH^ will be
converted to N0«.  Some of the nitrogen may  be used by plants, some may
be lost by denitrification as molecular nitrogen  and nitrogen oxides or
volatilization as ammonia  (NHO, and  some may be leached down  to the
ground water as NO,.

     A limit of 10 yg/ml of nitrogen  as NOZ plus NOg  ion is recommended
as a domestic water supply standard (EPA 1976) .  Waters containing N03 plus
                                     33

-------
CO
        TABLE 11.   CHEMICAL ANALYSIS OF  RAW SEWAGE FROM  FEBRUARY  18,  1976  TO NOVEMBER 11,  1976, COLLECTED
                     FROM THE SEWAGE TREATMENT PLANT

Date


760218b
750225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
7C0722
760805
760331
760916
760928
761019
761103
761111
Mean
Maximum
Minimum
Standard
Annual ii

Time


850
903
920
935
1020
845
930
945
1000
820
825
915
855
948
940
1015
945
915
900
1012
1012
905
1005



Deviation
iput* 103

Temp

°C
19.0
18.4
20.8
14.0
19.3
20.4
19.3
20.5
22.7
21.8
23.0
25.0
26.0
27.8
26.5
26.8
28.2
23.0
27.9
26.0
23.9
19.8
20.6
22.9
28.2
14.0
3.9
Kg/year

PH


7.4
7.0
7.1
7.6
7.3
7.2
7.2
7.3
7.1
7.4
7.1
7.2
7.3
7.0
7.3
7.1
7.0
7.2
7.3
7.4
7.1
7.1
7.4
7.2
7.6
7.0
0.2


•NHj-N

/ig/nl
18.2
18.0
17.1
29.2
16.2
18.1
17.7
16.0
13.2
17.1
18.8
15.7
15.5
13.8
13.7
16.3
13.6
12.9
14.5
15.1
15.8
12.0
15.5
16.3
29.2
12.0
3.4
112.6

NOj-N

;ug/ml
0.1
0.1
ND
Nt>
ND
ND
ND
ND
0.1
ND
ND
1.5
0.2
0.1
0.2
0.3
0.1
0.5
0.3
0.1
0.4
0.2
0.2
0.2
1.5
<0.1
0.3
1.4

NOj-N

ng/ml
ND0
ND
7
9
ND
6
8
7
11
9
19
21
ND
19
7
10
11
8
30
53
32
11
11
13
53
< 5
12
0.090

Kjeldahl
N
Dissolved
Ortho-P

Total
P


Zn Cu

	 Tig/ml 	 	 	 •
24.6
27.0
27.4
36.4
23.8
24.9
23.7
23.0
20.0
23.9
26.6
20.7
22.5
19.2
17.0
19.2
22.2
16.9
16.6
17.6
20.2
17.2
19.6
22.2
36.4
16.6
4.6
153.3
5.25
5.48
5.25
13.30
5.63
5.50
5.75
5.38
4.25
7.33
5.58
5.00
4.75
4.13
4.37
4.37
4.25
3.75
3.75
3.13
3.55
1.28
3.75
5.08
13.30
3.13
2.05
35.1
12.00
11.25
10.25
22.25
11.25
14.00
31.63
12.38
8.00
14.13
1Q.75
9.63
7. 03
8.88
7.00
8.00
6.90
5.88
5.25
7.50
7.68
6.75
7.75
9.83
22.25
5.25
3.71
67.9
110 120
200 130
170 120
48 64
130 100
115 92
325 100
155 83
178 87
109 92
191 80
117 108
190 87
79 78
94 76
90 93
140 73
142 78
96 87
190 81
87 76
70 82
120 70
137 89
325 130
43 64
59 17
0.946 0.615

Hi Cd

	 ng/ml — ---
ND 8
ND 7
15 ND
IV ND
15 ND
15 ND
10 5
20 5
15 SD
20 8
15 ND
25 8
30 ND
12 5
10 ND
33 ND
19 4
«D 14
ND ND
ND ND
12 5
14 5
17 5
14 ^4
33 14
<10 <4
9 4
0.097 ~

Cr Pb


375 ND
320 80
225 230
80 100
140 ND
150 170
205 100
540 ND
440 50
420 ND
660 ND
400 ND
400 ND
140 ND
210 ND
200 KD
ND ND
330 50
ND 50
425 50
200 ND
200 ND
145 ND
272 <50
660 230
<. 5 <50
169 62
0.188 ~
          a Assume 5 mgd (6.908 x 10' liters/year) incoming sewage effluenc.
           Year, month, day
         0 Not detectable

-------
TABLE 12.  CHEMICAL ANALYSIS OF WATER SAMPLES FROM NOVEMBER 18,  1975 TO NOVEMBER 11,  1976
             TAKEN FROM LAGOON NO. 1 LOCATED ON THE SAN ANGELO SEWAGE FARM

Date

751118s
751125
751202
751209
751216
751222
760101
760103
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
75 1019
761103
761111
Mean
Maximum
Minimum
Standard

Time

415
845
1000
925
915
900
1014
1050
1245
1013
1100
900
845
840
930
855
905
950
830
945
1030
945
845
800
845
910
1015
905
940
920
850
845
950
948
845
915



deviation

Temp
°C ~
18.0
12.0
10.0
9.5
11.0
8.9
8.2
3.8
9.1
8.8
8.2
11.0
13.0
13.2
11.2
1S.O
14.2
13.0
15.9
15.8
18.0
22.2
20.0
19.0
24.4
24.3
25.8
26.6
26.2
27.8
26.0
24.7
22.1
15.8
13.0
19.2
16.3
27.8
3.8
6.6

PH

7.2
7.2
7.3
7.4
7.4
7.3
7.2
7.3
7.4
7.5
7.5
7.4
7.6
7.4
7.2
7.0
7.1
7.3
7.2
7.2
7.3
7.1
7.3
7.3
7.6
7.2
7.5
7.5
7.1
7.1
7.1
7.2
7.4
6.3
7.5
7.0
7.3
7.6
7.0
0.2
I
NHj-N
jug/ml
29.7
27.8
28.1
30 1
28.6
28.8
30.8
30.0
32.5
33.8
32.6
33.4
33.2
33.4
34.0
30.7
32.3
33.0
32.3
35.1
40.3
37.3
36.5
33.5
23.7
24.0
21.1
20.1
18.5
19.3
18.1
19.0
17.5
18.7
18.6
20.3
23.2
40.3
17.5
6.6

NO^-N
,ug/i»l
NDb
0.4
0.5
0.4
0.2
0.2
0.1
0.2
0.1
0.2
0.3
0.2
ND
ND
ND
0.1
NT)
0.2
0.1
0.1
0.1
0.1
0.1
0.2
0.7
0.3
0.3
0.7
0.3
0.3
0.4
0.5
0.2
0.2
0.3
0.2
0.2
0.7
< 0.1
0.2

NO~-N
ng/ml
5
NP
6
5
4
ND
9
23
ND
15
5
ND
ND
ND
8
S
8
ND
It
8
19
20
11
9
13
13
5
29
29
23
10
34
45
8
16
16
12
45
< 5
11

Kjeldahl


34.9
39.9
40.1
40.7
39.9
39.3
37.2
29.1
40.1
35.4
39.9
38.0
35.7
41.1
46.5
45.3
42.8
45.9
42.8
47.8
44.0
48.4
55.3
45.6
45.2
47.2
57.5
44.2
33.8
36.4
36.4
24.0
35.2
37.9
31.7
36.1
40.7
57.5
24.0
6.6
Dissolved
Ortho-P


11.25
11.50
11.98
10.20
13.00
13.30
14.00
15.30
14.50
15.50
15.75
16.25
15.75
14.75
16.48
16.00
15.50
16.03
15.00
13.75
15.00
13.75
13.25
9.00
8.00
6.63
1.25
2.00
4.75
6.63
7.75
7.50
6.45
7.75
5.50
6.75
. 11.35
16.48
1.25
4.36
Total
P


Zn


Cu Ni


Cd


Cr Pb


14.63
40.63
19.00
30.00
21.13
13.80
25.00
58.80
18.25
29.80
29.30
24.88
22.73
28.75
26.25
27.50
32.50
27.50
28.13
29.38
27.50
26.25
21.25
18.75
21.87
21.25
29.75
23.03
24.00
22.00
21.00
18.25
19.50
19.00
18.40
20.00
24.94
58.80
13.80
8.02
350
330
240
450
220
170
80
80
100-
150
140
120
180
100
150
230
246
140
165
105
111
111
245
130
111
135
130
73
1540
80
93
106
118
62
62
150
194
1540
62
246
110 ND
•140 40
200 ND
110 20
103 20
80 ND
60 ND
50 ND
50 20
50 20
60 ND
20 30
70 20
70 20
60 20
110 20
130 13
57 20-
92 45
70 20
65 25
58 15
95 20
49 20
51 ND
54 20
57 15
56 16
8060 2200
50 42
48 10
64 10
50 10
37 12
60 20
118 20
296 77
8060 2200
20 <10
1331 364
ND
ND
ND
ND
15D
ND
ND
ND
ND
ND
ND
ND
ND
12
8
5
ND
ND
ND
6
5
5
10
6
7
6
ND
ND
ND
4
ND
ND
5
5
ND
5
<4
12
<4
3
200 150
150 100
150 150
300 200
150 150
100 100
80 150
70 ND
70 KD
80 ND
100 ND
80 ND
100 ND
140 50
140 ND
210 230
190 230
80 70
120 170
95 70
102 ND
105 ND
230 120
88 70
90 ND
88 50
80 50
70 ND
65 ND
66 50
150 50
240 100
120 175
90 ND
95 ND
180 ND
124 69
300 230
65 <50
57 75
* Year, month, day
Not detectable

-------
           TABLE 13.  CHEMICAL ANALYSIS OF WATER SAMPLES FROM NOVEMBER  18,  1975 TO NOVEMBER 11,  1976
                        TAKEN FROM LAGOON NO.  4  LOCATED ON THE SAN ANGELO SEWAGE FARM
UJ

Date
7511188
751125
751202
751209
751218
751222
760101
760103
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760326
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Mean
Maximum
Minimum
Standard

Time
405
130
230
150
155
1225
155
315
415
310
415
1245
120
135
230
120
215
340
1000
1045
230
415
1115
925
1035
1000
1130
320
1135
140
1035
1100
1118
207
1030
1135



deviation

Temp
°C
18.0
10.5
17.0
14.5
14.4
10.8
8.8
6.5
9.2
11.0
11.2
15.0
15.0
19.5
14.5
21.0
17.0
21.7
17.0
17.0
22.0
26.0
22.8
20.3
23.0
24.8
27.9
32.6
27.8
30.0
28.0
26.3
22.2
17.3
15.0
16.0
18.7
32.6
6.5
6.5

PH
8.0
8.2
7.8
7.3
8.2
7.9
7.2
8.0
7.7
7.7
7.6
7.8
7.9
7.6
7.5
7.7
7.9
7.7
7.6
7.5
7.8
7.9
8.0
7.6
7.1
7.6
_..|_
7.6
7.2
7.4
8.2
7.5
7.8
7.4
8.0
7.8
7.7
8.2
7.1
0.3
4
NH4-N
;ug/ml
19.4
20.8
23.4
25.7
26.4
30.3
26.0
26.2
26.9
27.7
27.6
26.6
27.7
26.5
25.7
24.6
24.6
24.9
22.5
24.0
21.7
11.7
19.1
ia.fi
17.5
16.3
14.4
11.8
13.4
12.8
11.7
13.4
15.0
14.5
15.2
17.9
21.1
30.3
11.7
5.5

NOj-N
/ig/ml
M,"
0.3
0.5
0.7
0.2
0.7
0.2
0.3
0.1
0.1
0.1
0.2
ND
0.1
ND
0.1
ND
0.1
ND
ND
0.1
0.1
0.2
0.3
2.5
0.4
0.3
0.3
0.6
0.3
0.4
0.2
0.3
0.3
0.4
0.3
0.3
2.5
<0.1
0.4

NOj-N
ng/ol
5
5
16
5
13
6
6
8
9
9
ND
6
6
13
17
6
18
6
56
10
17
19
11
23
125
20
ND
50
26
34
5
19
14
18
15
14
18
125
<5
22

Kjeldahl
29.9
29.0
31.6
28.8
31.7
33.6
31.5
32.0
32.2
28.5
32.3
29.1
22.6
33.5
33.8
33.7
32.9
35.4
34.1
33.6
28.8
31.2
30.9
28.8
35.4
32.7
39.3
25.2
24.1
24.6
20.4
26.1
23.7
26.2
22.0
23.0
29.8
39.3
20.4
4.4
Dissolved
Ortho-P
3.34
8.75
8.13
8.60
10.00
11.00
10.50
11.30
12.30
12.00
9.50
12.75
12.75
12.25
12.50
12.00
12.00
11.00
11.00
11.50
9.38
4. ^0
2.00
1.50
5.50
4.88
4.00
0.13
2.15
6.00
5.00
0.17
3.75
1.88
3.45
5.38
7.58
12.75
0.13
4.16
Total
P
9.63
50.00
20.00
22.50
18.40
12.50
21.30
21.30
12.50
25.00
25.00
20.38
20.13
23.13
17.50
18.60
22.50
21.30
18.13
20.63
19.75
12.50
15.00
13.75
23.12
13.75
21.50
16.00
17.50
14.50
13.00
11.75
15.10
12.50
12.00
12.00
18.45
50.00
9.63
6.92

Zn
110
90
110
280
140
80
70
60
70
120
110
40
140
70
20
180
258
720
77
92
410
238
290
150
74
175
160
440
72
.320
120
85
92
207
53
160
163
720
20
138

Cu
120
80
80
80
60
60
50
ND
40
30
60
ND
70
70
50
50
54
54
51
54
46
42
46
40
40
42
37
38
76
160
25
31
36
31
58
46
53
160

-------
           TABLE 14.  CHEMICAL ANALYSIS OF WATER  SAMPLES FROM NOVEMBER 18,  1975 TO NOVEMBER 11,  1976
                     TAKEN  FROM SEEPAGE CREEK NO.  1  ON THE SAN ANGELO SEWAGE  FARM
U>
Date


751118*
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Mean
Maxijnuni
Minimum
Standard
Time


210
1125
1235
1200
1130
1030
1157
105
230
1215
140
1045
1050
1115
1225
1150
1150
115
200
100
1200
400
1000
1155
1110
1115
225
1155
110
130
1135
1145
100
1248
1230
120



deviation
Temp
__ 	
\j
21.0
12.5
16.0
14.0
13.0
14.0
14.7
13.0
16.2
13.0
13.8
14.5
14.5
13.5
14.8
20.2
16.4
19.5
19.0
20.0
18.8
28.8
17.2
23.5
21.7
21.6
26.9
24.4
23.1
30.7
26.5
25.8
20.9
7.0
zO.4
17.4
18.8
30.7
12.5
4.9
PH


7.5
7.4
7.6
7.6
7.3
7.4
7.4
7.5
7.6
7.7
7.4
7.7
7.3
7.5
7.0
7.4
7.3
7.5
7.6
7.7
7.2
7.6
7.3
7.7
7.5
8.0
7.5
7.6
7.1
7.6
7.7
7.4
7.5
7.7
7.5
7.6
7.5
8.0
7.0
0.2
T +.»

^Jg/ull
b
ND
8S
ND
ND
ND
ND
ND
2.7
ND
ND
ND
0.3
ND
ND
ND
2.1
1.7
1.4
0.6
ND
1.0
ND
3.5
0.7
ND
ND
3.6
0.6
ND
ND
ND
ND
ND
ND
ND
ND
0.5
3.6
< 0.3
1.0
NOj-N

^Jg/raJ.
6.9
10.3
9.4
12.8
7.4
11.2
4.6
15.4
11.8
11.8
12.4
12.0
9.6
9.4
8.6
8.0
10.2
9.8
9.4
10.0
9.8
10.2
11.4
15.6
12.6
11.2
6.4
11.6
12.8
9.8
J2.2
15,0
10.2
13.2
8.2
8.S
10.6
15.6
4.6
2.4
NOj-N

us/mi
55
44
18
41
58
51
90
73
101
75
115
148
148
160
160
275
193
33
163
225
188
36
1325
275
613
200
288
169
187
281
156
153
150
73
113
143
183
1325
18
224
Kjeldahl


1.1
1.2
1.9
0.9
1.1
2.0
0.6
4.3
0.8
0.6
1.3
0.7
0.9
0.9
2.8
2.7
3.2
2.7
1.7
1.0
1.0
1.2
4.9
1.6
2.1
1.0
6.3
2.2
1.3
1.4
0.6
0.7
0.8
0.7
-
0.4
1.7
6.3
0.4
L.3
Dissolved
Ortho-P


0.15
0.11
0.15
0.19
0.27
0.18
0,20
0.81
0.43
0.16
0.16
0.09
0.00
0.14
0.85
0.51
0.89
0.81
0.11
0.14
0.29
0.19
0.34
0.33
0.65
0.51
1.80
0.65
0.57
0.30
0.10
0.13
0.13
0.07
0.30
0.32
0.36
1.80
< 0.01
0.35
Total
P


0.19
0.21
0.37
0.20
0.33
0.18
0.28
0.48
0.48
0.63
0.50
0.24
1.03
0.45
1.43
1.90
1.90
0.81
0.14
0.48
0.60
0.43
2.60
0.60
1.83
1.00
3.80
1.30
0.95
0.55
0.15
0.13
0.23
0.76
0.37
0.58
0.79
3.80
0.13
0.78
Zn


30
20
90
70
110
30
70
ND
20
180
20
30
20
20
100
60
28
120
135
78
56
214
64
155
73
220
205
120
110
400
162
84
112
82
65
170
98
400
< 10
79
Cu


30
ND
30
20
30
30
20
20
20
20
20
ND
20
20
ND
ND
15
15
ND
11
16
20
18
10
16
13
14
14
30
20
10
13
14
31
20
20
17
31
<10
9
Nt


ND
110
20
ND
30
ND
ND
20
ND
30
ND
30
30
30
30
15
13
20
30
15
30
25
25
25
15
30
16
20
46
24
20
13
20
20
26
26
22
110
<10
19
Cd


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
8
14
10
ND
ND
ND
ND
5
5
6
6
6
a
10
ND
ND
ND
4
ND
5
ND
5
5
7
< 4
14
< 4
4
Cr


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
7
5
5
ND
ND
ND
ND
ND
ND
ND
5
11
ND
9
5
ND
ND
5
6
ND
10
ND
< 5
11
< 5
3
Pb


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
70
ND
ND
ND
ND
ND
ND
ND
ND
50
ND
ND
ND
ND
ND
ND
50
ND
ND
ND
< 50
70
< 50
16
           Year, month, day
           Not detectable

-------
           TABLE 15.  CHEMICAL ANALYSIS OF WATER SAMPLES  FROM NOVEMBER 18, 1975 TO NOVEMBER 11,  1976
       TAKEN FROM SEEPAGE  CREEK NO. 2 LOCATED ON THE  CONCHO RIVER ADJACENT TO THE SAN ANGELO SEWAGE FARM
oo

Date

751118*
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760i21
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Mean
Maximum
Minimum
Standard

Time

150
1245
215
1245
1225
1104
1245
200
310
125
215
1130
1135
1145
100
1035
1050
200
1245
1230
100
330
1015
1140
1215
120
310
130
220
220
1215
1245
1246
1235
1145
1240



deviation

Temp
°C
21.5
20.0
20.0
20.0
19.9
19.7
20.0
19.0
19.5
19.7
19.5
19.0
18.8
18.8
17.8
21.0
18.4
18.9
18.4
13.5
19.0
19.3
18.2
18.0
18.4
18.9
18.4
19.6
19.6
19.5
19.3
19.7
19.8
20.8
19.0
20.0
19.3
21.5
17.8
0.8

PH

7.9
7.1
7.1
7.1
7.2
7.7
7.1
7.0
7.2
7.3
7.6
7.4
7.3
7.3
7.0
7.3
7.3
7.3
7.4
7.2
7.0
7.2
7.1
7.3
7.6
7.5
7.1
7.3
7.2
7.4
7.5
7.2
7.1
7.2
7.5
7.1
7.3
7.9
7.0
0.2
-1-
NH ,-N
4
/is/ml
NT,"
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
< 0.3
<0.3
<0.3
0

NOj-N
,Ug/ml
10.5
10.5
13.6
14.8
12.0
16.2
16.2
30.0
17.0
10.6
18.4
17.4
15.4
16.5
16.0
14.0
17.5
16.0
15.5
14.0
15.5
15.5
15.0
15.0
14.5
16.5
17.5
18.0
21.0
14.5
20.0
20.5
16.0
20.0
19.0
16.5
16.3
30.0
10.5
3.5

N02-N
ng/ml
ND
ND
5
5
ND
ND
ND
ND
ND
8
ND
ND
ND
ND
ND
5
8
ND
6
13
ND
5
7
6
16
ND
5
5
9
6
ND
10
6
6
7
ND
< 5
16
< 5
4

Kjeldahl
N

ND
0.4
ND
0.4
ND
1.2
0.3
ND
ND
ND
0.3
ND
ND
0.4
ND
ND
ND
1.0
ND
ND
ND
ND
0.3
0.3
ND
0.3
0.4
0.4
ND
0.4
ND
0.4
0.5
0.6
ND
ND
< 0.3
1.2
< 0.3
0.3
Dissolved
Ortho-P
	 -/is/ml 	
0.02
0.05
0.04
0.04
0.04
0.04
0.05
0.06
0.05
0.06
0.06
0.04
ND
0.05
0.05
ND
0.05
0.04
0.03
0.03
0.04
0.04
0.04
0.03
O.C6
0.05
0.04
0.02
0.03
0.04
0.03
0.02
0.01
0.04
0.01
SO
0.04
0.06
< 0.01
0.02
Total
P

Zn

Cu

Ni

Cd

Cr Pb

0.05
0.05
0.05
0.06
0.06
0.04
0.05
0.06
0.05
0.07
0.06
0.04
0.04
0.05
0.05
ND
0.05
0.04
0.03
0.03
0.04
0.04
0.04
0.03
0.07
0.05
0.04
0.02
0.03
0.04
0.03
0.02
0.01
0.04
0.01
ND
0.04
0.07
< 0.01
0.02
40
30
20
70
60
20
10
20
30
40
20
40
20
10
90
20
10
330
80
120
96
188
66
150
108
315
215
315
175
350
222
163
75
68
54
140
105
350
10
100
ND
ND
ND
30
40
20
ND
30
ND
ND
20
ND
ND
30
ND
ND
ND
11
19
ND
ND
18
18
10
ND
10
11
17
32
14
10
13
14
SD
20
10
11
40
< 10
11
ND
ND
ND
ND
30
ND
ND
ND
40
20
ND
ND
20
ND
20
ND
17
20
15
10
20
25
20
25
15
20
15
16
37
24
10
ND
17
20
23
20
14
40
< 10
11
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
12
14
6
ND
ND
ND
ND
ND
ND
5
6
6
7
5
ND
ND
ND
ND
ND
ND
ND
5
ND
5
< 4
14
< 4
4
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
7 ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
m ND
ND ND
ND ND
ND ND
ND 50
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
< 5 < 50
7 50
< 5 < 50
1 8
        " Year, month, day
        ° Not detectable

-------
OJ
         TABLE 16.   CHEMICAL ANALYSIS  OF WATER SAMPLES FROM NOVEMBER  18,  1975 to NOVEMBER 11,  1976
           TAKEN FROM SEEPAGE CREEK NO.  3 LOCATED ON PRIVATE LAND ON  THE  CONCHO RIVER ADJACENT
                                         TO THE SAN ANGELO SEWAGE FARM

Date


7511183
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
76021S
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760525
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Mean
Maximum
Minimum
Standard

Time


1035
940
1040
1015
945
920
1050
1125
115
1040
1140
935
920
945
1000
950
1005
1050
930
1015
1030
200
905
900
955
935
1050
1030
1050
1030
1000
1000
1051
1042
940
1050



deviation

Temp

"C
19.5
18.5
19.0
19.0
18.2
18.0
18.9
18.6
18.0
18.8
18.8
19.0
19.0
19.0
13.9
19.0
19.0
19.9
18.2
18.5
18.1
20.2
18.8
19.0
18.0
19.1
19.0
19.5
19.6
19.4
19.8
19.6
19.2
18.9
19.3
18.8
18.9
20.2
18.0
0.5

pH


7.1
7.1
7.2
7.4
7.1
7.2
7.1
7.0
7.2
7.3
7.0
7.6
7.2
7.4
7.0
7.1
7.4
7.4
7.3
7.1
7.0
7.1
7.2
7.7
7.6
7.5
7.2
7.5
7.5
7.1
7.5
7.1
7.2
7.0
7.1
7.3
7.2
7.7
7.0
0.2

NH^-N
4
pg/ml
ND*
ND
ND
KD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
<0.3
<0.3
<0.3
0

NOj-N

jug/ml
17.6
13.6
17.0
20.8
15.2
19.4
20.5
6.0
24.0
24.5
22.5
20.0
21.5
22.5
20.5
21.0
24.5
20.5
22.5
22.5
24.0
22.5
23.0
21.5
25.0
24.0
24.5
22.5
23.5
20.0
23.0
21.0
19.0
25.5
19.0
21.5
21.0
25.5
6.0
3.7

M>2-N

ng/ml
9
ND
5
5
ND
ND
ND
ND
ND
5
ND
ND
ND
ND
ND
ND
ND
6
ND
10
7
8
9
9
6
5
ND
5
9
6
5
12
6
6
7
ND
< 5
12
< 5
4

K.ji:ldahl
M

ND
0.4
ND
0.4
ND
1.2
0.5
ND
ND
ND
ND
ND
0.5
0.4
0.5
ND
ND
ND
0.3
ND
SD
ND
ND
0.4
NH
0.3
ND
ND
SD
ND
SD
0.4
ND
0.4
0.5
ND
<0.3
1.2
<0.3
0.3
Dissolved
Ortho-P


0.02
0.03
0.02
0.03
0.03
0.03
0.04
0.02
0.02
0.03
0.04
0.02
0.02
0.03
C.03
0.02
O.i12
0.05
NP
0.05
0.02
• 0.03
0.03
0.03
0.04
0.03
XD
0.01
0.02
0.03
0.02
ND
ND
0.01
0.01
ND
0.02
0.05
<0.01
0.01
Total
P


0.02
0.03
0.02
0.03
0.03
0.03
0.04
0.03
0.04
0.04
0.04
0.02
0.02
0.03
0.07
0.02
0.04
0.05
ND
0.05
0.02
0.03
0.03
0.03
0.04
0.06
0.03
0.01
0.02
0.05
0.03
Nl>
ND
0.01
0.01
ND
0.03
0.07
<0.01
0.02

Zn


40
50
50
90
60
60
20
40
60
100
150
90
130
20
70
20
58
99
69
120
35
119
23
97
60
135
71
77
45
115
84
44
55
77
31
96
71
150
20
35

Cu


20
ND
30
40
50
30
ND
ND
ND
ND
20
ND
20
20
ND
ND
11
ND
ND
11
10
18
10
10
ND
ND
17
14
50
14
ND
13
18
ND
17
10
13
50
<10
14

Hi

	 Hj
ND
ND
20
ND
20
ND
ND
20
20
20
ND
ND
20
ND
20
10
10
ND
20
15
25
20
20
25
25'
30
16
20
37
15
13
10
10
17
26
20
15
37
<10
10

Cd

!/nl 	
ND
ND
ND
ND
ND
ND
ND
Ml
ND
ND
ND
ND
12
12
8
ND
ND
5
5
5
5
5
12
8
10
5
ND
ND
ND
4
ND
ND
ND
6
ND
7
<4
12
< 4
4

Cr


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
5
ND
ND
ND
ND
ND
ND
ND
ND
<5
6
<5
1

Pb


ND
HD
ND
ND
ND
ND
' ND
ND
SO
ND
HP
ND
ND
ND
ND
ND
ND
ND
ND
ND
SD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
<50
<50
<50
0
         Year, month, day
        b Not detectable

-------
TABLE  17.  CHEMICAL ANALYSIS OF WATER SAMPLES  FROM NOVEMBER 18,  1975 TO NOVEMBER 11,  1976 TAKEN
            FROM CONCHO  RIVER SAMPLING SITE NO.  1 LOCATED UPSTREAM  FROM THE SAN ANGELO  SEWAGE FARM

-Date

751118b
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760S05
760?31
760916
760928
761019
761103
761111
Mean
Maximum
Minimum
Standard

Time

945
815
920
830
840
750
940
1030
1030
940
1045
845
830
825
845
840
850
935
815
840
915
930
805
745
240
825
925
845
915
900
830
830
934
932
822
900



Deviation

Temp
°C
16.5
9.5
9.5
10.0
9.3
8.7
8.0
4.0
6.7
7.5
8.2
10.0
13.0
13.0
10.7
18.8
13.8
14.0
15.3
14.2
18.0
21.2
19.4
18.5
28.0
25.2
28.0
27.0
26.4
28.0
25.3
26.0
21.7
15.9
13.3
15.0
15.8
28.0
4.0
7.0

PH

8.1
8.2
8.1
8.1
8.2
8.2
8.2
8.1
8.0
8.2
8.1
8.2
8.2
8;2
7.9
8.1
8.'2
8.1
8.0
8.0
8.1
8.1
8.4
8.2
8.3
8.1
8.4
8.0
7.9
8.0
8.2
7.8
7.8
7.8
7.8
8.2
8.1
8.4
7.8
0.2

NH*-N
Aig/ral
NDC
ND
ND
ND
ND
ND
0.3
ND
ND
ND
ND
0.7
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
1.0
ND
ND
ND
<0.3
1.0
<0.3
0.2

NO~-N
.Ug/ml
1.0
2.4
2.6
2.4
1.9
2.8
2.1
4.8
2.8
2.6
1.6
2.0
1.9
1.5
2.8
2.5
1.3
1.3
1.3
1.3
0.8
0.9
0.7
0.6
0.7
0.6
0.8
0.3
0.7
0.9
0.8
0.8
0.9
1.5
1.1
1.4
1.6'
4.8
0.3
0.9

NO^-N
ng/nl
19
18
28
15
14
19
25
25
20
23
25
18
20
25
34
28
34
26
28
43
31
43
14
18
71
26
44
15
30
28
28
32
26
22
20
16
27
71
14
11

Kjeldahl
rl

0.5
0.4
0.6
0,7
0.4
1.7
0.8
!O
0.5
0.3
0.5
1.1
0.8
0.7
0.6
to
0.6
1,0
0.3
0.4
0.6
0.5
0.4
0.8
1,1
0.8
1.7
0.9
0.6
0.4
1.0
0.5
0.8
0.8
0.5
0.6
0.7
1.7
<0.3
0.4
Dissolved
Ortho-P
'--- /ig/ml — -
ND
0.03
0.02
0.01
0.02
0.02
0.01
0.02
ND
0.01
0.03
0.02
0.02
0.06
0.02
ND
0.02
0.03
0.03
0.01
0.02
0.02
0.02
0.02
0.01
0.01
ND
0.03
0.04
ND
0.01
0.01
ND
0.01
0.01
0.01
0.017
0.060
<0.010
0.013
Total
P

0.06
0.06
0.09
0.01
0.06
0.03
0.05
0.05
0.06
0.08
0.05
0.05
0.07
0.06
0.06
0.05
0.07
0.04
0.03
0.06
0.07
0.14
0.12
0.07
0.09
0.10
0.10
6.04
0.04
0.08
0.03
0.01
0.03
0.06
0.04
0.02
0.060
0.140
<0.010
0.029

Zn

40
50
30
70
80
30
10
20
30
120
10
90
30
20
80
10
23
10
12
35
24
64
29
76
380
63
27
24
30
42
32
22
19
23
18
40
48
380
10
63

Co

130
20
ND
50
40
20
30
30
ND
20
20
30
20
20
ND
ND
15
ND
21
11
32
12
18
21
ND
10
17
ND
66
14
ND
10
12
ND
23
ND
20
130
< 10
24

Ni
	 ng,
40
ND
40
20
ND
ND
ND
ND
30
ND
ND
ND
ND
ND
ND
ND
13
ND
ND
ND
15
ND
10
20
ND
15
ND
ND
87
ND
NI)
ND
ND
ND
10
10
<10
87
{ 10
18

Cd
/ml 	
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
5
5
5
5
ND
ND
ND
5
ND
ND
ND
ND
ND
ND
<4.0
5.0
<4.0
1.8

Cr Pb

ND ND
20 ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND 70
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND' ND
ND ND
ND ND
5 ND
ND ND
ND ND
ND ND
<5.0 <50
20.0 70
<5.0 <50
3.4 12
  a Significantly different from downstream water samples
  b Year, month, day
  c Not detectable

-------
TABLE  18.   CHEMICAL ANALYSIS  OF WATER  SAMPLES FROM NOVEMBER  18,  1975 TO  NOVEMBER  11,  1976 TAKEN
            FROM CONCHO RIVER  SAMPLING  SITE NO. 2 LOCATED DOWNSTREAM FROM THE SAN  ANGELO SEWAGE FARM

Date


751118b
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Mean
Maximum
Minimum
Standard

Time


310
115
150
130
1240
1117
100
215
325
140
230
1145
1215
1220
120
1205
1205
205
1115
1145
115
345
1030
1045
125
135
325
155
235
240
1245
100
110
103
1255
135



Deviation

Temp

°C
18.0
12.0
13.0
13.0
13.0
10.0
10.9
7.2
10.9
10.3
11.0
13.0
15.0
16.5
14.8
21.0
16.2
17.1
17.8
16.9
20.0
25.0
20.6
19.9
25.8
26.9
27.4
28.8
26.4
30.8
26.6
27.2
22.1
17.2
17.8
15.8
18.2
30.8
7.2
6.3

pH


8.0
7.9
8.0
8.0
8.2
8.0
7.9
8.0
7.9
8.1
8.2
8.1
8.0
7.9
7.9
7.8
8.0
8.1
7.9
8.0
7.9
8.0
8.2
8.1
8.2
8.1
8.0
7.8
7.9
8.1
8.0
7.8
7.3
7.7
7.9
7.9
8.0
8.2
7.7
0.1.



/ug/ml
NDC
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
0.4
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
0.3
ND
ND
ND
ND
0.7
ND
ND
ND
<0.3
0.7
<0.3
0.1

NOJj-N

jug/ml
3.9
6.0
6.6
6.0
5.7
7.3
6.3
14.0
8.2
8.2
5.6
6.6
6.0
5.1
6.3
5.8
6.5
5.9
6.3
6.1
3.3
3.4
5.1
3.4
4.3
5.0
7.1
3.1
3.6
6.3
3.5
4.5
4.5
5.4
3.9
5.1
5. f
14.0
3.1
2.0

NOI-N

rig/ml
24
33
38
16
19
23
29
31
31
31
24
25
31
38
45
50
59
44
50
80
50
78
75
80
185
•75
120
45
65
93
30
56
60
29
24
33
51
185
16
33

KJeldahl
H

ND
0.6
0.6
0.6
0.5
1.3
0.5
0.5
0.7
0.7
0.6
0.9
0.9
0.8
0.6
ND
0.6
1.4
1.0
0.7
0.5
0.7
0.7
0'.9
1.0
1.8
1.7
1.0
0.9
0.3
ND
0.6
0.7
0.9
ND
0.4
0.7
1.8
<0.3
0.4
Dissolved
Ortho-P

	 jug/ml— — •
0.02
0.02
0.19
ND
0.02
0.01
0.01
0.05
0.01
0.01
0.02
0.02
0.02
0.02
0.05
0.03
0.03
0.05
0.02
0.03
0.02
0.03
0.02
0.02
0.03
0.01
ND
0.01
0.02
ND
0.01
ND
ND
0.02
0.04
0.01
0.024
0.190
< 0.010
0.031
Total
P


0.06
0.06
0.19
0.10
0.06
0.04
0.06
0.06
0.14
0.14
0.07
0.06
0.08
0.07
0.06
0.08
0.07
0.05
0.03
0.09
0.08
0.11
0.18
0.14
0.10
0.12
0.12
0.05
0.05
0.06
0.03
0.03
0.05
0.04
0.07
0.01
0.078
0.1SO
0.010
0.042

Zn


40
50
60
80
60
40
20
20
30
50
20
40
40
20
110
90
119
290
60
125
157
215
89
185
150
235
275
200
140
500
236
181
95
82
102
210
123
500
20
101

Cu


20
ND
40
30
30
ND
ND
ND
ND
20
30
20
ND
ND
ND
ND
ND
ND
11
15
15
15
13
13
10
10
19
12
20
17
10
16
18
11
15
ND
12
40
4.10
11

Nl


20
20
ND
ND
ND
ND
ND
ND
20
ND
ND
ND
ND
ND
ND
KD
ND
20
ND
10
15
15
15
20
ND
20
10
10
24
19
ND
ND
ND
ND
ND
14
<10
24
< 10
9

Cd

-ng/ml--
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
8
ND
ND
ND
ND
5
ND
ND
ND
5
12
5
10
ND
ND
7
ND
ND
ND
ND
5
ND
ND
< 4.0
12.0
<4.0
3.2

Cr


ND
ND
ND
ND
ND
10
ND
ND
ND
ND
ND
ND
ND
ND
ND
5
TO
5
ND
ND
ND
ND
ND
ND
ND
7
ND
ND
5
ND
ND
ND
5
ND
ND
ND
<5.0
10,0
<5.0
2.5

Pb


KD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
<50
<50
<50
ND
     Significantly different from upstream water samples.

    b Year, month, day
    c Not detectable

-------
          TABLE 19.   CHEMICAL ANALYSIS OF WATER SAMPLES FROM DOMESTIC DEEP WELLS
                        AND SHALLOW GROUND WELLS AT THE SEWAGE FARM
Date

Time

Temp.
°C
pH

NHt-N
/ig/ml
NO~-N
Jig/ml
NOj-N
ng/ml
Shallow Ground Well -
751118 a
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760521
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
230
1055
1200
1120
1105
1005
1138
1240
150
1145
115
1025
1020
1045
1055
1120
1120
1205
115
130
1130
1030
945
1220
1050
1050
155
1140
1245
105
1115
1130
1152
1137
1115
100
18.5
16.0
16.5
15.5
15.0
14.5
14.1
12.3
13.7
12.2
13.2
11.3
13.5
14.0
12.3
16.4
16.6
16.5
17.2
16.0
16.6
23.1
16.8
19.7
21.9
20.4
21.8
23.9
23.2
22.0
23.0
25.3
22.0
17.9
17.0
15.3
7.4
7.3
7.6
7.6
7.3
7.1
7.5
7.5
7.4
7.8
8.1
7.7
7.5
7.6
7.4
7.4
7.2
7.4
7.1
7.2
7.1
7.0
7.3
7.0
7.9
7.7
7.3
7.6
7.1
7.2
7.7
7.5
7.6
7.1
7.5
7.3
NDb
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
0.6
1.0
ND
ND
ND
6.2
8.4
1.7
1.1
0.8
4.4
0.9
1.3
0.2
0.7
0.7
0.5
1.9
0.2
0.3
0.1
1.5
0.9
3.4
2.8
3.0
12.8
4.6
0.8
6.7
2.2
2.2
6.9
6.4
4.2
0.6
0.5
0.3
2.2
2.1
4.1
26
8
5
8
ND
28
ND
10
13
6
ND
ND
17
ND
ND
5
725
20
363
40
88
43
700
125
575
150
215
323
537
75
10
20
15
111
75
78
Kjeldahl
N

Dissolved
Ortho-P
..*. /~i
Total
P

Zn

Cu

Nl
«« /
Cd
'_1
Cr

Pb


Location No.
1.5
0.7
ND
0.5
ND
1.2
0.7
ND
ND
SD
ND
ND
NP
0.4
ND
IID
ND
ND
0.3
ND
ND
ND
ND
ND
0.7
0.7
ND
0.3
ND
ND
ND
ND
1.3
ND
0.7
ND
8
0.04
ND
0.01
0.01
0.01
0.01
0.04
ND
0.02
ND
0.02
0.02
0.02
0.01
0.01
0.02
ND
0.04
0.03
0.02
0.02
0.03
0.02
0.03
0.03
0.02
ND
0.01
0.02
ND
ND
H>
ND
0.01
0.02
0.01

0.59
0.22
0.14
0.01
0.19
0.30
0.08
0.07
0.03
0.06
0.07
0.04
0.20
0.02
0.02
0.03
0.08
0.04
0.03
0.02
0.05
0.03
0.02
0.03
0.04
0.04
0.03
0.01
0.02
0.02
0.02
to
ND
0.01
0.02
0.01

50
280
500
200
150
90
110
30
120
240
250
200
240
70
140
120
228
160
235
275
155
105
165
355
195
295
341
255
220
470
286
166
190
157
48
352

20
230
100
120
120
90
70
30
80
120
100
50
140
80
70
210
145
120
70
212
150
36
340
177
165
280
242
275
102
110
158
92
230
120
120
116

ND
ND
20
20
30
ND
40
20
30
ND
ND
ND
20
ND
20
ND
ND
25
10
10
25
25
20
25
25
20
18
16
46
19
13
10
10
20
26
20

ND
ND
10
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
12
7
ND
ND
ND
5
5
•6
5
8
8
10
6
ND
ND
ND
5
ND
ND
ND
8
ND
5

ND
ND
20
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
8
6
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
TO
ND
ND
ND
ND

ND
ND
150
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Year, month, day
Not detectable
                                                                                     (continued)

-------
          TABLE 19.   (continued)
UJ
Date


751118s
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
Tine


245
1110
1220
1135
1120
1021
1147
115
220
1200
130
1035
1035
1100
1110
1135
1135
100
100
115
1145
1100
955
1205
1100
1105
215
1210
100
120
1125
Temp.
°C

18.5
16.2
15.5
16.0
15.1
15.0
16.0
13.2
16.1
14.6
19.2
15.6
16.1
11.2
15.3
18.8
16.6
16.8
17.3
19.3
18.0
23.5
18.3
20.0
19.8
21.0
21.9
22.9
22.0
24.0
22.5
pH


6.9
7.3
7.7
7.1
7.7
7.0
7.2
7.7
7.5
8.0
7.6
7.5
7.4
7.2
7.4
7.1
7.1
7.2
7.1
7.1
7.1
7.2
7.1
7.1
7.6
7.5
7.0
7.3
7.2
7.2
7.5
NH+-N
Jig /ml

NDb
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6.3
NOj-N
/ig/ml
Shallow
8.1
9.2
2.6
12.7
11.8
13.8
9.2
14.4
6.2
2.0
4.9
8.6
5.5
4.0
5.0
5.5
10.8
10.0
2.6
11.1
10.2
12.6
U.4
3.8
14.0
12.2
13.8
13.0
10.6
10.0
6.6
NOj-N
ng/ml
Ground Well
25
25
10
10
14
23
20
33
36
8
9
28
26
37
21
250
46
10
50
63
60
50
75
300
238
350
350
400
525
388
30
Kjel.iahl
N


- Location
ND
0.7
1.1.
0.3
0.4
1.1
0.7
1.0
0.5
ND
0.3
BD
0.5
1.3
M)
iV
ND
i..l
ND
IID
HD
ND
0.4
NT*
HD
KD
0.3
:ro
:JD
3.5
6.6
Dissolved
Ortho-P


No. 17
0.08
0.10
0.04
0.06
0.05
0.04
0.05
0.06
0.01
0.03
0.02
0.03
0.03
0.02
0.01
ND
0.02
0.02
0.03
0.01
0.05
0.06
0.05
0.05
0.04
0.03
0.01
0.03
0.05
0.02
0.06
Total
P



0.09
0.35
0.07
0.07
0.08
0.04
0.24
0.15
0.05
0.04
0.04
0.05
0.16
0.03
0.03
0.02
0.04
0.02
0.03
0.01
0.05
0.15
0.08
0.05
0.04
0.07
0.09
0.03
0.05
0.04
0.07
Zn



60
240
10
270
130
50
210
70
120
190
270
110
180
50
100
80
156
210
165
125
112
140
180
340
180
225
325
225
190
480
232
Cu



40
230
ND
140
280
100
310
40
100
60
100
190
340
60
60
60
94
290
92
162
125
71
350
238
190
195
347
115
130
160
142
Nl



ND
40
ND
ND
20
ND
20
ND
30
ND
ND
20
20
ND
ND
10
ND
10
20
10
25
25
25
25
38
15
22
20
46
24
ND
Cd
no /ml ...


ND
10
ND
ND
ND
ND
ND
ND
ND
ND
ND
HD
8
ND
8
ND
ND
ND
ND
6
6
6
8
6
12
6
14
ND
5
4
ND
Cr



10
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
8
ND
6
5
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Pb



ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
70
ND
ND
ND
ND
ND
50
ND
           a Year, month, day
           b Not detectable
                                                                                                       (continued)

-------
 TABLE 19.   (continued)
Date


751216s
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Time


110
1140
120
230
335
155
300
1200
1235
1235
135
1220
115
230
1015
1100
130
1130
1045
950
145
150
340
220
250
255
100
115
200
121
115
150
Temp
°C

19.0
18.5
12.6
12.0
18.1
11.8
16.5
16.8
16.2
18.5
17.6
17.8
16.0
16.3
16.8
16.5
17.5
20.2
17.5
17.3
19.0
19.2
19.7
21.5
21.0
20.1
19.8
21.6
20.0
20.7
20.0
18.5
pH


7.4
7.4
7.1
7.0
7.4
7.3
7.0
7.6
7.0
7.0
7.0
7.1
7.1
7.1
7.2
7.0
7.0
7.4
7.3
7.6
7.2
7.3
7.1
7.2
7.2
7.0
7.4
7.0
7.1
7.0
7.0
7.0
NH+-N
>ig,'«il

ND*
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO~-N
ml
Shallow
0.8
1.4
0.7
1.4
0.7
1.3
1.6
1.8
0.9
1.3
1.0
0.8
10.8
2.0
0.3
1.6
0.8
0.8
0.2
0.8
1.9
1.1
0.7
1.1
2.1
1.3
0.8
0.5
0.5
1.3
0.4
0.3
SO^-N
ng/ml
Ground
5
8
20
7
13
28
ND
15
11
5
8
11
47
9
12
14
13
29
37
45
26
5
9
9
8
11
ND
12
14
11
7
ND
Kjeldahl
N

Well - Location
0.6
1.4
0.5
0.4
0.4
ND
0.3
0.5
0.4
0.5
0.5
N3
NO
O.o
ND
ND
ND
1.0
0.4
0.6
0.8
0.4
0.5
NO
N3
NO
N'3
0.4
NO
S3
0.5
N3
Dissolved
Ortho-P
— -/jg/ml 	
No. 18
0.05
0.09
0.10
0.10
0.13
0.15
0.11
0.10
0.09
0.10
0.10
.14
0.02
0.13
0.09
0.08
0.14
0.16
0.11
0.12
0.15
0.16
0.13
0.10
0.13
0.11
0.14
0.07
0.08
0.05
0.10
0.01
Total
P


0.05
0.09
0.12
0.15
0.14
0.15
0.13
0.10
0.09
0.10
0.10
0.14
0.04
0.13
0.09
0.08
0.14
0.17
0.11
0.12
0.16
0.16
0.13
0.10
0.13
0.11
0.14
0.07
0.08
0.13
0.10
0.10
Zn


180
60
70
60
90
110
120
80
110
70
180
120
132
310
125
225
202
275
258
248
245
550
465
370
370
650
400
320
192
197
206
330
Cu


310
90
110
80
130
100
90
60
160
150
90
90
87
92
140
173
135
234
490
257
165
670
147
160
180
102
122
142
180
100
145
140
Nl
	 ng/ml

20
ND
20
20
40
ND
ND
ND
ND
ND
ND
15
ND
10
15
15
20
25
15
15
25
30
16
10
42
15
ND
101
10
29
29
26
Cd


ND
ND
ND
ND
ND
10
ND
ND
10
ND
ND
ND
ND
ND
ND
5
5
9
6
6
8
5
ND
ND
5
ND
ND
ND
ND
6
7
7
Cr


ND
ND
ND
ND
ND
ND
ND
ND
6
ND
7
ND
ND
ND
5
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Pb


BD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
b Year, month, day
  Not detectable
(continued)

-------
TABLE 19..   (continued)
Date


7512168
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
Time


120
1155
125
240
345
1200
310
1210
1245
1245
150
1235
130
245
1030
1110
145
1145
1050
1000
1155
200
350
230
305
310
115
130
205
130
130
Temp
°C

19,0
17.0
16.3
11.5
17.4
14.6
16.0
15.0
15.7
18.7
16.2
17.2
17.7
16.8
16.8
16.2
16.9
21.2
16.8
17.0
18.2
17.7
18.4
20.1
21.0
21.7
19.2
20.3
19.9
20.3
18.7
PH


7.1
6.9
7.0
7.0
7.1
7.2
7.0
7.3
7.0
7.0
7.0
6.9
7.0
7.0
7.0
7.0
6.3
7.2
7.0
7.3
7.4
7.0
7.0
7.2
7.0
7.0
7.0
7.0
7.0
7.0
7.1
NH+-N
jig/ml

**
ND
ND
ND
m
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO^-N
^Jg/ral
Shallow
1.6
4.0
1.3
2.3
0.8
1.7
1.7
1.5
1.6
1.1
1.2
1.1
1.1
0.4
0.4
1.4
1.5
2.0
2.2
2.4
3.7
3.9
4.2
2.9
4.7
6.0
6.0
8.5
6.6
7.4
1.4
NOj-N
ng/ral
Ground Well
ND
ND
10
5
9
5
14
ND
ND
ND
ND
5
39
11
6
16
7
11
15
43
10
6
14
9
12
13
13
12
11
9
19
Kjeldahl
S

- Location I
ND
0.9
0.7
ND
ND
ItD
0.3
ND
tro
0.4
;ro
ND
ND
0.5
1JD
:n>
iffi
KD
HD
0.4
KD
:m
ai>
SD
ND
D.5
SC
D.3
:XD
TO
0.5
Dissolved
Ortho-P
---- *ig/ml-----
(o. 19
0.03
0.03
0.03
0.18
0.02
0.04
0.04
ND
0.02
0.02
0.02
0.02
0.03
ND
0.02
0.01
0.03
0.02
0.03
0.04
0.03
0.04
0.02
0.03
0.02
0.01
0.03
ND
0.01
0.03
0.18
Total
P


0.03
0.03
0.05
0.18
0.04
0.04
0.04
0.02
0.03-
0.02
0.02
0.02
0.07
ND
0.03
0.01
0.03
0.02
0.03
0.04
0.03
0.05
0.02
0.03
0.02
0.01
0.03
0.01
0.01
0.05
0.18
Zn


150
140
90
50
100
120
130
70
110
60
150
120
218
410
86
130
257
215
270
247
170
580
475
435
390
970
446
273
181
187
246
Cu


170
400
90
40
240
150
120
50
100
60
70
60
83
150
135
200
157
74
420
210
125
400
3J2
150
215
102
122
142
180
100
145
Nl


30
ND
40
20
50
ND
ND
ND
ND
20
20
25
10
10
20
20
25
25
20
20
38
35
18
16
50
15
ND
13
ND
26
35
Cd
ng/ml--— •

ND
ND
ND
ND
ND
ND
ND
ND
12
ND
9
ND
ND
5
ND
6
5
6
6
6
12
6
8
ND
5
4
ND
KD
ND
6
11
Cr


ND
ND
ND
ND
ND
10
ND
ND
7
6
7
5
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
Pb


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
50
ND
50
ND
ND
 a Year, month, day
 b Not detectable
                                                                                             (continued)

-------
TABLE 19 .    (continued)
Date


76Ulf
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
76092S
761019
761103
761111
Time


205
130
1200
130
250
355
230
320
1220
1255
100
155
1250
145
310
1045
1120
200
1200
1055
1010
205
210
400
245
320
325
130
145
211
133
145
220
Temp
°C

18.0
19.0
19.0
18.6
17.0
18.6
18.3
17.7
18.0
18.1
17.9
19.2
19.1
18.0
19.8
16.8
17.0
18.7
20.4
15.9
16.8
18.1
17.9
18.0
22.1
19.8
20.2
20.8
20.1
19.0
20.9
20.0
18.0
pH


7.0
7.0
6.9
7.0
7.1
7.1
7.1
7.0
7.3
7.0
7.0
6.9
7.0
7.0
7.1
7.0
6.9
6.8
7.0
7.0
7.0
7.2
7.0
7.0
7.0
7.0
7.2
7.1
7.0
7.0
7.0
7.3
7.0
NH*-N
(Jg/ml

b
ND
ND
ND.
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO"-N
.ug/ml
Shallow
4.4
4.4
6.6
5.2
7.2
5.1
4.3
4.9
5.0
4.3
3.8
3.9
3.8
4.1
2.4
0.7
4.2
3.9
5.0
5.1
4.2
8.0
7.3
7.4
7.0
10.1
6.8
10.0
9.5
7.6
10.8
8.2
7.6
NO"-N
ng/ml
Ground
11
11
6
6
8
5
8
ND
ND
ND
ND
13
5
8
6
7
13
7
14
16
23
16
5
20
7
15
21
6
13
10
13
5
ND
KJeldahl
N

Well - Location
ND
0.3
0.7
0.4
ND
ND
HD
ND
0.5
0.4
0.4
0.4
KD
ND
0.6
ND
ND
ND
J;D
ND
ND
ND
ND
NO
ND
ND
ND
ND
0.3
ND
0.3
ND
ND
Dissolved
Ortho-P
	 /ig/ro.l 	
No. 20
0.01
0.02
0.03
0.03
0.02
0.01
0.03
0.02
ND
0.01
0.03
0.02
KD
0.02
0.04
0.02
0.03
0.02
0.02
0.04
0.04
0.04
0.04
ND
0.01
0.02
0.01
0.02
0.01
KD
0.02
0.03
KD
Total
P


0.01
0.02
0.03
0.03
0.05
0.03
0.03
0.02
0.01
0.01
O.Q3
0.02
ND
0.05
0.04
0.07
0.03
0.02
0.02
0.04
0.04
0.08
0.07
0.01
0.01
C.02
0.03
0.02
ND
0.03
0.02
0.07
ND
Zn


525
180
50
90
50
70
150
190
80
100
80
180
120
168
540
125
170
275
178
270
235
330
515
465
600
340
660
402
360
235
207
216
365
Cu


140
190
100
120
70
70
150
170
70
100
130
90
60
74
60
120
185
120
36
350
210
320
235
187
120
81
225
128
104
166
105
110
175
Ni
	 ng/ml

26
20
ND
40
20
40
ND
ND
ND
ND
20
20
25
20
15
20
20
40
30
30
20
38
35
18
20
42
15
ND
16
10
20
20
26
Cd


8
ND
ND
ND
ND
ND
ND
ND
ND
12
8
10
ND
ND
8
ND
8
6
6
11
6
10
ND
5
ND
5
ND
ND
ND
ND
6
11
8
Cr


ND
10
ND
ND
ND
ND
10
ND
ND
8
6
7
5
ND
ND
5
ND
ND
tJD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Pb


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
70
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
50
ND
ND
ND
ND
Year, month, day
Not detectable
(continued)

-------
TABLE 19,  (continued)
Kjeldnhl Dissolved
Date


751216*
751222
760101
760108
760112
760121
760128
760X04
760211
760218
760225
760103
760110
760U5
760324
760331
760'.07
760414
760121
760110
760526
760f,016
760928
761019
761103
761111
Time


140
1215
140
300
405
250
345
1230
105
115
210
105
200
325
1100
1130
215
1215
1100
1020
215
220
410
300
335
340
145
200
222
144
200
235
Temp
o
C

18.0
18.0
18.8
18.5
18.1
17.7
17.8
18.0
17.7
18.8
18.6
21.2
17.2
17.2
16.8
16.8
18.1
21.1
16.5
17.2
19.3
18.9
19.5
21.0
20.8
19.8
19.7
20.5
19.3
20.9
19.5
16.6
pH


7.0
7,0
7,0
7.3
7.1
7.3
7. 1
7.2
7.0
7.3
7.0
7.0
7.1
7.1
7.1
7.2
7,2
6,8
6.9
7.2
7.2
7.2
7.0
7.1
7.1
7.1
7.1
7.1
7.1
7.1
7.4
7-2
NH+-N
fig/ml

b
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
KD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NOj-N
/Jg/nii
Shallow
10.2
12.0
8.4
18.0
14.4
15.6
12.0
8.4
11.2
13.0
13.6
16.0
8.8
4.8
8.2
11.6
12.2
12.2
13.2
15.6
17.4
18.8
15.0
16.0
17.4
10.8
16.2
20.0
16.5
25.0
11. 0
14.0
NOj-N
ng/ml
Ground
6
6
6
8
8
9
ND
ND
ND
ND
6
8
8
6
ND
8
9
20
15
16
31
11
6
36
238
ND
20
13
24
14
5
SD
N
• •— -- .
Ortho-P
	 . — jUg/ml 	 •
Total
p
	

Zn


Cu


Ni


Cd
ng/ml---

Cr


Pb

Well - Location no. 21
ND
1.0
0.5
ND
ND
ND
0.3
ND
ND
0.7
ND
ND
ND
ND
ND
NO
SD
(TO
SD
0.7
ND
0.5
ND
ND
0.6
ND
NO
0.3
0.4
1.5
ND
ND
~ SIJAUJOW r.F.OlINTT
All Sainpl«s (N
Mean
Maxinum
MinlTTMm
Standard
* Vear.



deviation
month, day
18.1
25.3
11.2
2.6

7.2
8.1
6.8
0.2

<0.3
6.3
<0.3
0.5

5.8
25.0
0.1 <
5.2

49
725
5
118

< 3.3
6.6
<: 0.3
0.6

0.01
0.03
0.02
ND
0.02
0.02
0.02
ND
0.01
0.02
0.02
ND
0.02
ND
0.01
0.03
0.03
0.03
0.02
0.04
0.02
0.03
ND
ND
0.02
0,01
0.02
0.01
ND
0.03
0.01
ND
'WEES " ~~~
• 195)
0.04
0.18
<0.01
0.04

0.02
0.03
0.03
0.04
0.03
0.03
0.02
0.02
0.01
0.02
0.02
ND
0.06
ND
0.01
0.03
0.02
0.03
0.02
0.04
0.02
0.09
0.02
ND
0.02
0.02
O.'.O
0.01
0.01
0.04
0.01
ND

0.06
0'.59
<0.0i
0.07

180
40
70
50
100
110
120
70
100
80
170
140
208
740
115
125
310
175
310
210
335
920
465
740
500
710
400
445
210
402
316
3 HO

233
970
10
165

20
50
70
60
130
70
100
50
110
120
60
70
64
90
115
150
180
31
565
225
230
310
222
94
150
295
78
224
144
94
181
145

149
670
< 10
97

20
ND
40
ND
30
ND
ND
Nn
20
20
30
25
13
10
20
15
20
20
25
15
38
35
18
16
42
29
ND
16
10
20
23
23

17
50
<10
13

ND
ND
ND
ND
ND
ND
ND
ND
12
6
8
ND
ND
ND
ND
8
6
6
8
6
12
ND
6
ND
7
ND
5
ND
ND
8
7
7

<4
14
<4
4

20
ND
ND
ND
ND
10
SD
ND
u
6
8
NO
ND
ND
5
ND
ND
NO
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

<5
20
<5
3

ND
NP
ND
ND
ND
ND
ND
ND
ND
ND
ND
70
70
ND
ND
ND
ND
NT)
ND
ND
ND
ND
ND
ND
ND
ND
ND
K>
ND
ND
ND
ND

<50
150
<50
16

Not detectable /___*-,•„ 	 i\

-------
         TABLE  19.   (continued)
oo
Date


751118"
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Time


1010
835
945
905
900
852
1004
1045
1230
955
1115
915
855
900
915
910
920
1005
900
930
1015
855
815
900
840
1000
955
955
1000
930
915
1026
1022
915
1025
Temp.
°C

22.0
13.0
19.0
19.5
18.5
19.6
21.0
18.2
17.2
8.3
19.0
19.0
14.5
20.5
13.0
20.3
14.0
19.6
21.5
16.8
20.5
19.0
20.2
2A.O
25.0
25.4
26.0
24.2
26.3
25.0
26.8
21.2
19.0
20.5
20.7
PH


7.3
7.3
7.3
7.2
7.4
7.3
7.2
7.1
7.4
7.7
7.2
7.4
7.0
7.4
7.0
7.3
7.3
7.3
7.2
7.1
7.2
7.6
7.5
7.6
7.6
7.4
7.5
7.5
7.3
7.4
7.3
7.2
7.0
7.2
7.5
NH+-N
/ig/ml

ND»
ND
ND
•ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO~-N
/ig/ml
Domestic
4.8
9.3
7.6
9.0
5.8
11.2
3.6
14.0
9.6
10.0
9.4
8.4
8.6
8.4
8.0
6.8
8.6
7.8
8.2
6.2
8.8
8.8
8.0
9.6
8.4
9.2
7.4
10.8
8.2
10.2
10.8
8.6
14.4
9.0
10.4
NO~-N
ng/ml
Deep Well
ND
ND
5
5
ND
ND
ND
5
ND
13
ND
ND
11
ND
ND
ND
5
ND
ND-
ND
5
40
9
6
ND
10
ND
5
ND
ND
12
8
8
9
5
Kjeldahl
N

- Location
1.1
ND
ND
ND
0.4
1.0
0.3
ND
ND
ND
ND
ND
0.5
ND
ND
ND
ND
ND
ND
ND
ND
ND
0.3
ND
ND
ND
ND
ND
ND
ND
0.3
0.3
ND
ND
ND
Dissolved
Ortho-P
.... — pg/iol- 	 --
No. 9
ND
ND
ND
ND
0.01
0.01
0.02
ND
0.01
0.03
0.02
0.01
ND
0.03
ND
ND
ND
0.02
0.03
0.01
0.02
0.02
ND
0.02
0.01
ND
0.02
0.02
0.01
0.03
ND
ND
0.01
ND
ND
Total
P


ND
ND
ND
ND
0.02
0.01
0.03
ND
0.03
0.04
0.02
0.01
0.03
0.03
0.01
ND
0.01
0.02
0.03
0.01
0.02
0.02
0.03
0.03
0.01
0.01
0.02
0.02
0.01
0.03
ND
ND
0.01
ND
ND
Zn


120
120
100
140
90
110
60
310
70
120
140
160
180
70
150
70
48
117
93
118
200
175
160
155
200
120
140
110
250
190
180
262
377
66
138
Cu


50
ND
60
40
30
ND
20
»
ND
20
20
ND
20
30
ND
20
15
15
21
30
28
13
13
16
45
35
30
66
40
16
34
30
15
24
22
Hi
	 ng/ml

40
ND
40
ND
ND
ND
170
ND
30
20
ND
ND
ND
ND
ND
ND
10
15
20
25
20
20
25
45
15
18
16
42
24
10
10
17
20
20
20
Cd


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
10
6
6
ND
ND
5
ND
9
5
6
6
8
6
19
ND
ND
8
ND
ND
ND
6
ND
5
Cr


10
ND
10
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
5
5
ND
ND
ND
ND
ND
ND
ND
m
Pb


ND
ND
ND
SD
ND
ND
ND
ND
ND
ND
150
ND
ND
ND
ND
ND
70
SO
70
ND
ND
ND
70
ND
50
ND
ND
50
50
ND
ND
ND
ND
ND
ND
         * Year, month, day
         b Not detectable
                                                                                                      (continued)

-------
         TABLE 19.  (continued)
-o
VO
Date

Time

Temp
°C
PH

<*
/ig/ml
NOj-N
Jig /in I
NOJ-N
ng/ml
Domestic Deep Well
751118 a
751125
751202
751209
751216
751222
760101
760108
760112
760121
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
h Year,
1025
920
1025
1000
935
825
1035
1115
105
1030
925
910
930
945
935
950
1035
915
1000
1015
145
900
850
940
925
1035
1015
1030
1015
950
940
1044
1032
920
1040
month, day
20.0
18.5
19.5
19.5
19.2
19.4
19.7
13.0
19.5
17.7
19.1
19.6
19.8
19.2
18.6
19.2
20.6
19.0
19.5
18.7
20.5
19.3
20.0
21.0
19.0
20.8
20.5
20.5
21.1
21.0
21.7
19.8
19.1
19.0
19.0

7.0
6.9
7.0
7.1
7.0
7.0
7.1
7.1
7.2
7.3
7.3
7.0
7.0
7.0
7.2
7.2
7.3
7.0
7.2
7.0
7.1.
7.0
7.5
7.2
7.2
7.2
7.5
7.8
7.2 ,
7.1
7.1
7.1
7.2
7.1
7.0

NDb
ND
ND
ND
ND '
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND.
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

15.8
13.6
15.4
18.0
15.0
22.0
21.0
22.0
24.0
21.5
19,0
21.0
23.5
23.5
24.0
23.0
23.0
24.5
22.0
24.5
25.0
23.5
19.0
25.0
23.5
24.0
23.0
27.0
20.0
26.0
30.0
22.5
30.5
20.0
25.4

5
ND
5
5
ND
ND
5
8
ND
11
ND
ND
ND
13
10
9
ND
6
7
9
11
9
11
16
8
9
5
7
12
ND
12
10
6
4
ND

Kjeldahl
N

- Location No
0.4
ND
ND
0.3
ND
1.1
0.3
ND
ND
ND
ND
ND
0.3
0.3
ND
NO
NO
0.3
ND
ND
ND
ND
ND
0.4
ND
ND
ND
ND
ND
ND
ND
0.5
ND
NO
NO

Dissolved
Ortho-P
— -- jig/ml----
. 10
0.03
0.03
0 02
0 04
0.03
0.03
0.02
6.01
0.02
0.03
0.02
0.02
ND
0.01
0.03
0.02
0.03
ND
0.01
0.03
0.05
0.04
0.02
0.04
0.03
ND
0.02
0.02
0.02
0.02
ND
ND
0.01
0.01
0.01

Total
P
Zn
Cu
Ni
Cd
Cr
Pb
... 	 — , 	 . 	 ng/ml 	 	

0.03
0.03
0.02
0.04
0.04
0.03
0.05
0.05
0.03
0.04
0.02
0.03
0.01
0.01
0.03
0.02
0.03
N)
0.01
0.03
0.05
0.04
0.02
0.05
0.04
0.02
0.02
0.02
0.02
0.02
ND
ND
0.01
0.01
0.01


0.5
150.0
80.0
270.0
200.0
190.0
280.0
20.0
190.0
300.0
280.0
300.0
180.0
300.0
200.0
241.0
350.0
225.0
240.0
255.0
275.0
145.0
255.0
250.0
295.0
102.0
182.0
190.0
225.0
162.0
163.0
180.0
147.0
123.0
255.0


50
70
20
20
20
20
20
20
ND
ND
ND
ND
ND
ND
ND
24
ND
ND
ND
10
23
13
10
ND
13
14
17
91
22
19
ND
20
ND
17
20


ND
ND
ND
ND
20
ND
20
ND
40
20
ND
ND
ND
20
10
10
10
10
20
20
20
20
20
25
15
16
16
46
19
10
13
17
20
25
23


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
10
10
ND
ND'
5
5
5
ND
5
8
5
8
5 .
5
ND
ND
4
ND
ND
ND
6
5
ND


ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
5
ND
ND
ND
ND
ND
ND
ND
ND


ND
ND
ND
100
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO
ND
ND
ND
ND
ND
50
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

0 Not detectable /„„„*. ,'«.,^J\

-------
         TABLE 19.   (continued)
Ul
o
Date


751118°
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
Time


200
1210
130
110
1140
1041
1210
130
245
1255
155
1100
1105
1130
1140
1105
1205
130
1130
1200
1215
300
1005
1100
1145
1125
240
1225
1230
145
1230
1200
1202
1152
1200
Temp
°C

19.5
18.0
19.0
19.0
19.1
19.0
19.1
17.3
19.8
18.9
18.8
19.5
18.5
18.5
18.6
19.8
19.8
20.1
19.6
16.9
18.2
19.7
18.7
17.9
20.2
19.0
19.8
13.8
19.0
19.8
19.0
19.8
19.3
18.8
19.5
PH


7.0
7.1
7.0
7.2
7.1
7.0
7.0
7.1
7.2
7.0
7.1
7.3
7.0
7.1
6.9
7.0
7.2
7.1
7.0
7.0
7.0
7.1
7.0
7.2
7.3
7.3
7.1
7.4
7.2
7.1
7.3
7.0
7.3
7.1
7.0
NH£-N
pg/ml

NDb
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO
ND
SD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NOJ-N
*ig/ml
Domestic
8.1
9.6
11.8
12.0
8.8
13.6
11.6
18.6
15.6
15.6
13.8
13.8
13.0
12.6
12.2
13.2
14.2
12.6
13.6
13.6
14.4
15.0
14.2
17.8
16.4
15.8
15.0
14.0
16.4
12,4
14.8
17.0
10.8
16.6
11.2
NOJ-N
ng/ml
Deep Well
6
ND
5
5
ND
ND
13
7
ND
8
ND
ND
ND
ND
ND
11
8
8
8
11
ND
6
17
11
29
9
5
ND
9
11
ND
13
11
9
5
Kjeldahl
N
____..«___..
- Location
0.5
0.6
NI>
NH
KI>
1.1
0.4
Nt
Nt'
NT'
0.3
0.4
NT
0.4
NT
Nt
KC
RE
NE
NE
Nt
NE
NE
KC
NC
NE
NE
ND
ND
NE
1C
0.5
0.3
0.4
ND
Dissolved
Ortho-P
	 pg/ml 	 	
No. 11
0.02
0.02
0.02
0.03
0.03
0.03
0.03
0.02
0.01
0.03
0.04
0.03
0.03
0.02
0.02
0.01
0.04
0.02
ND
0.02
0.04
0.04
0.04
0.03
0.04
0.05
0.01
0.01
0.02
0.02
0.02
Nil
0.01
0.03
0,02
Total
P
*"*"*""" -

0.07
0.06
0.02
0-03
0.03
0.03
0.04
0.09
0.04
0.04
0.04
0.03
0.03
0.02
0.02
0.02
0.04
0.02
SD
0.02
0.04
0.06
0.08
0.04
0.04
0.05
0.01
0.01
0.02
0.02
0.02
ND
0.01
0.04
0.03
Zn
*.___

100
120
80
170
80
30
40
30
30
50
60
90
10
30
100
80
91
330
88
97
81
215
81
285
83
225
215
182
88
380
290
107
67
67
63
Cu
"*"""""*"

30
20
40
40
40
ND
ND
ND
ND
ND
20
ND
ND
KD
ND
ND
11
ND
NH
11
22
15
18
18
13
13
17
17
30
25
10
16
18
ND
20
Hi


KD
ND
ND
ND
30
ND
ND
20
30
20
ND
ND
ND
ND
20
15
10
10
10
15
25
20
20
20
32
15
16
10
37
15
10
13
20
20
20
Gd
ng/ml 	

ND
ND
ND
TO
KD
ND
ND
ND
SD
ND
ND
ND
11
14
6
ND
ND
ND
ND
5
5
6
6
6
12
8
ND
ND
ND
4
ND
ND
ND
5
9
Cr
	

ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
6
6
ND
ND
ND
ND
ND
ND
SD
ND
ND
ND
ND
ND
9
ND
ND
ND
ND
ND
ND
ND
Pb
	

ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
KD
ND
SD
ND
70
ND
ND
KD
1TD
ND
KD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
          Year, month, day
          Not detectable
(continued)

-------
TABLE 19,   (continued)
Date

Time

Temp
°C
pH

NH*-N
4
jig/ml
N05-N
/ig/ml
NOJ-N
ng/ml
Domestic Deep
751118a
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
1100
955
1100
1030
1000
935
1104
1140
125
1100
1220
950
935
1000
1015
1005
1020
1120
945
1030
1045
215
915
915
1005
945
1115
1050
1105
1045
220
1015
1104
1055
955
1105
20.0
20.0
21.0
20.5
21.0
20.0
20.9
19.2
20.3
19.7
20.3
20.0
19.9
19.3
19.1
18.8
19.0
19.1
19.0
18.9
19.0
19.9
18.7
18.8
18.2
18.8
18.8
19.6
19.2
20.2
20.5
20.0
19.0
19.4
19.9
19.6
7.1
7.0
7.2
7.1
7.1
7.2
7.2
7.1
7.2
7.4
7.1
7.6
7.0
7.0
7.0
7.2
7.2
7.2
7.3
7.2
7.1
7.0
7.1
7.1
7.6
7.3
7.0
7.4
7.3
7.2
7.1
7.2
7.2
7.1
7.1
7.2
NDb
ND
ND
;JD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
5ID
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
10.8
15.5
23.0
20.5
21.0
26.0
26.0
37.0
25.5
28.5
24.5
21.4
24.0
22.5
23.5
24.5
24.5
21.5
24.5
21.5
23.0
23.0
21.5
16.5
20.5
19.0
20.0
21.0
25.5
21.0
25.2
27.5
22.0
31.0
19.0
22.0
5
ND
5
5
ND
SD
9
ND
5
5
ND
ND
ND
ND
ND
ND
8
ND
6
11
7
11
ND
18
6
6
5
ND
9
6
ND
12
10
9
9
ND
Kjeld.ihl
N

Well - Location
ND
0.6
ND
0.4
0.4
1.0
0.3
ND
ND
ND
ND
ND
ND
0.3
ND
ND
ND
ND
ND
SD
ND
MD
ND
ND
ND
ND
0.4
ND
ND
0.3
ND
0.3
0.5
0.3
0.3
ND
Dissolved
Ortho-P
	 ^g/ml 	
No. 12
0.03
0.02
0.02
0.03
0.06
0.01
0.04
0.01
0.02
0.03
0.02
0.02
0.02
0.62
0.01
ND
ND
0.03
0.02
0.02
0.02
0.01
0.02
0.01
0.03
0.03
SD
0.01
0.02
0.02
0.02
ND
ND
0.01
0.02
ND
Total
P


0.03
0.02
0.05
0.03
0.06
0.01
0.04
0.04
0.02
0.03
0.02
0.02
0,02
0.02
0.01
ND
ND
0.03
0.02
0.02
0.02
0.01
0.02
0.01
0.03
0.05
ND
0.01
0.02
0.02
0.02
ND
ND
0.01
0.03
ND
Zn


20
90
80
110
80
70
40
60
40
140
180
60
120
30
110
40
10
85
41
52
40
208
33
93
77
101
88
74
55
155
540
50
39
55
29
170
Cu


ND
30
40
20
30
ND
ND
ND
ND
20
20
ND
20
ND
ND
ND
11
15
11
ND
22
18
13
10
10
10
11
14
40
22
10
22
18
13
17
15
Ni


ND
ND
ND
ND
ND
ND
ND
20
20
30
ND
ND
ND
ND
20
10
13
10
15
15
20
20
20
25
32
KD
17
16
42
24
10
13
ND
20
23
20
Cd
ng/ml 	

ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
10
7
4
ND
ND
ND
ND
5
5
5
100
8
10
ND
ND
ND
ND
4
6
ND
ND
5
ND
7
Cr


ND
ND
HI)
ND
ND
ND
ND
KD
ND
ND
ND
ND
ND
6
7
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Pb


ND
ND
ND
ND
KD
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
KD
  Year, month, day
b Not detectable
(continued)

-------
         TABLE 19_   (continued)
Ui
ho
^ Kjeldahl Dissolved
Date

751118"
751125
751209
751216
751222
760101
760108
760112
7C-0121
760128
760204
760211
760218
760225
760303
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
' Year,
Time

1130
1015
1045
1030
945
1112
1215
130
1115
1240
1005
950
1015
1030
1020
1035
1135
145
200
1100
230
925
1250
1020
1020
1145
1105
1120
1115
1015
1040
1111
1110
1035
1120
month, day
Temp
°C
20.0
19.0
19.5
17.2
19.5
12.1
12.5
19.9
19.1
19.4
20.0
19.1
19.3
17.9
20.7
18.3
19.9
19.5
19.3
17.3
20.1
18.4
19.3
19.0
19.7
19.4
22.2
21.6
24.7
20.0
19.5
18.3
19.2
19.1
18.7

pH

7.0
7.0
7.0
7.0
7.2
7.1
7.0
8.0
7.0
7.1
7.1
7.0
7.0
7.0
6.9
7.4
7.2
7.0
6.9
6.9
7.0
7.0
7.0
7.1
7.3
7.0
7.5
7.2
7.0
7.0
7.0
7.2
7.1
7.0
7.1

NHA-N
Jttg/nl
NDb
ND
ND
ND
M>
ND
ND
ND
ND
ND
ND
ND
ND
NI>
ND
ND
ND
KD
ND
ND
ND
ND
ND
W
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

N03-N
/Jg/ml
Domestic
5.1
9.8
14.8
8.2
11.8
13.6
16.2
14.4
17.2
16.6
16.0
8.8
13.8
7.8
8.4
11.4
12.2
12.0
10.2
7.2
15.2
10.2
16.6
19.0
17.5
14.0
16.0
20.0
14.5
10.0
12.5
20.5
22.0
13.0
20.5

N02-N
ng/ml
Deep Well
10
ND
5
9
6
103
35
ND
23
13
22
6
8
9
9
36
14
16
29
39
14
23
23
54
26
16
89
42
46
36
38
8
53
16
8

H
- Location No.
HD
0.4
0.5
KD
1.0
0.7
M>
fD
M)
>D
fiD
0.3
0.4
KD
ND
KD
ND
ND
ND
ND
0.4
ND
ND
ND
ND
0.3
0.3
N3
ND
N3
0.4
NO
10
Hi)
n>

Ortho-P
•-yug/ml-- — •
13
0.04
0.02
0.01
0.01
0.04
0.06
0.02
0.01
0.03
0.03
0.02
0.03
0.03
0.02
0.03
0.02
0.04
0.02
ND
0.02
0.02
0.03
0.04
0.03
0.03
ND
0.04
0.02
0.02
0.02
ND
ND
ND
0.03
KD

Total
P

0.04
0.05
0.01
0.03
0.04
0.06
0.10
0.04
0.04
0.03
0.02
0.03
0.03
0.02
0.03
0.03
0.12
0.04
ND
0.02
0.02
0.03
0.04
0.03
0,07
0.02
0.05
0.02
0.02
0.02
ND
ND
ND
0.03
ND


Zn

50
10
110
90
10
120
2250
40
150
180
170
130
20
90
40
44
62
250
90
68
173
50
305
75
175
104
160
120
240
125
49
92
80
96
238


Cu

30
20
20
40
20
40
ND
ND
30
20
20
20
ND
ND
ND
19
31
360
58
35
39
49
18
20
22
14
38
46
59
25
16
30
140
22
20


Ni

ND
ND
ND
20
ND
20
ND
40
ND
ND
ND
ND
ND
ND
15
ND
10
10
10
20
20
20
20
32
15
18
16
33
29
ND
13
10
23
26
23


Cd
•ng/ml---
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
8
8
7
ND
ND
ND
ND
ND
5
6
6
5
8
5
ND
5
ND
4
ND
ND
ND
5
ND
7


Cr Pb

ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND 100
ND 40
7 ND
6 ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND 70
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND ND
ND 50
ND NE
ND ND
ND ND
ND ND

b Not detectable .
                                                                                          (continued)

-------
             TABLE 19.   (continued)
01
Date



751118s
751125
751202
751209
751216
751222
760101
760108
760112
760121
760128
760204
760211
760218
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
Time



1155
1030
1125
1100
1050
957
1125
1225
140
1130
1255
1015
1005
1030
1150
130
145
1115
245
935
1235
1035
1035
'140
1125
1150
1245
1055
1115
1140
1120
1100
1150
Temp
n
°C

19.5
19.0
19.5
19.0
18.0
18.6
18.0
14.0
18.8
18.4
18.6
18.5
18.2
19.3
19.1
18.6
18.2
19.0
20.3
17.6
21.0
20.3
19.5
21.2
23.7
20.5
21.0
22.0
20.3
18.8
17.8
18.9
18.3
pH



6.9
7.0
7.1
7.0
7.3
7.0
7.0
7.7
7.2
7.1
7.0
7.2
7.0
7.2
7.0
7.0
6.9
7.0
6.9
7.2
7.2
7.4
7.5
7.1
7.6
7.2
7.3
7.3
7.2
7.2
7.0
7.1
7.2
NHj-N



TOb
ND
TO
ND
ND
TO
ND
TO
TO
ND
TO
TO
ND
TO
TO
ND
ND
ND
ND
TO
ND
TO
TO
TO
TO
TO
TO
ND
TO
ND
TO
ND
ND
NO"-N
I1O" /Tftl
A* (5 '
Domestic
10.8
10.8
10.6
9.6
9.2
11.4
12.2
24.0
14.8
14.4
11.6
9.6
11.2
11.6
8.4
2.6
11.8
9.8
11.0
11.0
18.0
19.0
19.5
16.5
17.0
20.0
16.0
17.5
18.5
13.0
18.0
13.0
14.0
NO"-N
f\n /inl
Ug/ HlL
Deep Well
24
13
63
15
19
20
31
105
26
34
23
36
23
20
18
16
40
19
23
16
60
69
13
13
18
19
9
8
12
8
9
13
TO

-------
           TABLE  19.   (continued)
Ui
+ Kjeldahl Dissolved
Date

Tine

Temp
°C
pH

NH. -N
4
Jig/ml
NO"-N
*lg/ml
NOj-N
ng/ral
Domestic Deep
751118*
751125
751202
751209
751216
751222
170108
760112
760121
760128
760i04
760211
7C0218
760225
760103
760310
760315
760324
760331
760407
760414
760421
760510
760526
760609
760624
760707
760722
760805
760831
760916
760928
761019
761103
761111
125
1150
125
1230
1200
1050
145
300
110
400
1115
1120
1235
1210
10SO
1105
145
1145
1245
1230
315
1010
1110
1200
105
250
1240
125
200
1150
1230
1235
1221
1130
1205
20.5
19.5
20.0
20.0
19.7
19.0
18.0
19.9
19.6
19.7
19.5
19.2
18.5
18.7
20.5
20.5
19.0
19.5
17.2
18.9
20.9
19.0
18.9
21.7
19.3
19.9
20.1
19.6
19.5
19.4
19.5
20.0
18.8
19.0
18.6
6.9
7.0
7.0
7.0
7.0
7.0
7.0
7.0
7.5
7.0
7.2
7.0
7.4
7.0
7.0
7.1
7.1
7.0
7.0
7.1
7.0
7.3
7.1
7.6
7.2
7.0
7.4
7.0
7.0
7.2
7.1
7.1
7.0
7.1
7.1
NDb
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
V)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NO
ND
ND
5.4
8.8
11.8
12.0
11.0
13.2
22.0
15. u
16.4
15.8
14.0
13.4
13.6
13.0
15.8
16.6
14.0
8.8
15.0
14.2
16.0
14.2
17.8
17.0
15.0
13.0
17.5
21.0
14.5
17.0
15.5
13.5
18.0
10.0
12.0
10
ND
5
5
ND
ND
10
6
8
ND
6
ND
ND
ND
9
19
6
9
16
11
14
ND
13
14
9
5
7
11
6
ND
12
6
6
0
ND
N

Well - Location no
2.6
0.4
NH
0.3
NP
0.9
W
HD
NH
ND
ND
0.5
0.3
0.4
HI'
NIi
0.5
KD
NTi
HD
NIi
ND
NIi
ND
HI'
MI
NT.
ND
HI'
Nl.'
0.4
NC
0.6
NC
NT
Ortho-P
«/•!
. 16
0.06
0.02
0.02
ND
0.02
0.02
0.02
0.01
0.04
0.03
0.03
0.02
0.02
0.03
0.01
0.03
ND
0.03
0.02
0.02
0.03
0.01
0.03
0.03
0.05
ND
0.02
0.02
0.01
0.02
ND
ND
0.02
0.01
MD
Total
P


0.06
0.02
0.02
ND
0.02
0.02
0.07
0.07
0.04
0.03
0.03
0.02
0.02
0.03
0.02
0.03
ND
0.03
0.02
0.02
0.03
0.01
0.03
0.05
0.07
0.03
0.02
0.02
0.02
0.02
ND
0.04
0.04
0.01
ND

Zn


60
30
50
100
70
60
60
20
50
100
80
30
30
110
60
33
220
130
103
109
133
67
180
150
285
265
225
205
510
252
190
63
102
136
225

Cu


20
ND
20
40
30
50
50
20
20
30
30
40
20
30
ND
19
15
15
19
19
23
18
10
23
10
17
17
50
25
12
16
14
11
20
15

Ni


20
ND
ND
ND
ND
ND
ND
40
ND
ND
ND
30
ND
ND
15
10
10
10
10
20
20
20
20
32
15
18
16
42
15
10
13
ND
23
23
23

Cd.


ND
10
ND
ND
ND
ND
ND
ND
ND
ND
ND
8
7
7
ND
ND
ND
ND
5
5
5
6
5
12
6
6
ND
ND
4
ND
ND
ND
5
5
7

Cr


10
ND
ND
ND
10
ND
ND
ND
ND
ND
ND
ND
6
6
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

Pb


ND
ND
ND
100
ND
ND
ND
SD
ND
SD
ND
ND
ND
ND
ND
70
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
50
ND
ND
SD
~ " DOMKSTIC DKF,:? WE11S

Mean
Maxlnura
Mini*™
Standard
h Year'




deviation
month, day

19.4
26.8
8.3
2.0


7.2
8.0
6.9
0.2


<0.3
<0.3
<0.3
<0.3


15.7
37.0
2.6
5.9

All
11
105
< 5
15

Samples (N - 249)
<0.3
2.£
<0.2
0.3


0.02
0,06
<0.01
0.01


0.03
0.12
<0.01
0.02


199
2250
< 10
264


21
360
<10
28


15
170
<10
16


<4
100
<4
7


<5
10
<5
2


<50
150
<50
21

Not detectable

-------
             TABLE 20.   CHEMICAL ANALYSIS  OF WATER SAMPLES  TAKEN FROM TOM GREEN COUNTY DEEP WELLS
Ui
Oi
Date



7603293
760520
760628
760714
760812
760921
761005
761122

760329
760520
760628

760329
760520
760628
760714
760812
760921
761005
761122

760329
760520
760628
760714
760812
760921
761005
761122

760329
760520
760628
760714
760812
760921
761005
761122
Tine



825
930
825
840
725
805
810
830

900
950
855

915
1005
910
910
800
835
835
900

940
255
920
950
815
900
849
930

1100
1125
945
1010
840
935
1001
950
Tenp.



18.4
21.0
23.3
22.7
24.5
21.5
19.0
16.0

21.3
21.8
21.5

17.2
20.8
22.0
24.2
25.8
20.8
20.5
18.4

19.6
23.8
24.5
24.1
26.0
23.0
21.9
15.6

22.0
28.8
24.8
22.6
22.5
22.2
21.5
19.0
PH



7.0
7.1
7.4
7.2
7.0
7.3
7.3
7.2

7.0
7.1
7.7

7.0
7.0
7.5
7.2
7.1
7.1
7.4
7.6

7.0
7.2
7.4
7.1
7.5
7.2
7.3
7.3

7.1
7.0
7.7
7.1
7.2
7.2
7.3
7.4
«£*

f-SI

N3Jb
ND
ND
ND
ND
ND
ND
ND

ND
ND
ND

ND
ND
ND
ND
ND
ND
ND
ND

ND
ND
ND
ND
ND
ND
ND
ND

ND
ND
ND
ND
ND
ND
ND
ND
NO^-N

/Ig/Ettl

7.0
4.7
4.9
3.8
4.2
6.5
10.5
8.8

22.5
25.0
26.5

35.5
26.5
23.5
34.5
35.5
45.5
80.0
45.0

10.0
26.5
11.5
22.5
12.5
29.5
38.0
29.5

2.4
2.5
2.4
1.3
2.9
4.1
7.0
5.6
NOj-fc

rig/ in j

ND
ND
ND
ND
ND
KD
ND
ND

ND
ND
ND

ND
9
41
39
8
ND
ND
ND

KD
ND
ND
ND
ND
8
5
18

ND
9
45
ND
20
ND
ND
ND
I Kjeldahl
N


LOCATION NO. 31
ND
ND
ND
ND
ND
ND
ND
ND
LOCATION NO. 32
ND
ND
ND
LOCATION NO. 33
ND
ND
0.5
ND
ND
ND
ND
ND
LOCATION NO. 34
ND
0.6
ND
ND
ND
0.4
ND
ND
LOCATION NO. 35
ND
ND
ND
ND
ND
ND
ND
ND
Dissolved
Ortho-P



0.05
0.04
0.02
0.02
ND
ND
0.01
ND

0.04
0.02
0.03

KD
0.03
0.01
0.01
ND
ND
0.01
ND

0.02
0.02
0.01
ND
ND
ND
0.01
ND

ND
0.02
0.01
0.01
0.01
ND
0.01
ND
Total
P



0.05
0.06
0.02
0.02
KD
ND
0.01
ND

0.04
0.02
0.03

ND
0.03
0.01
0.01
0.03
,ND
0.03
ND

0.03
0.03
0.01
ND
ND
ND
0.02
ND

ND
0.02
0.01
0.02
0.05
ND
0.03
ND
Zn



105
77
58
118
56
57
47
140

130
77
58

320
340
225
643
240
240
283
285

680
1600
438
783
302
1020
503
1210

460
1190
850
413
594
515
483
790
Cu



15
20
12
22
27
16
ND
14

15
20
12

11
13
12
11
ND
ND
ND
10

15
23
15
19
12
28
14
20

15
10
17
14
12
16
16
20
Ni



20
25
15
16
10
10
14
14

10
25
15

30
38
18
20
20
10
17
17

15
58
15
20
15
33
23
30

30
38
18
16
20
28
23
21
Cd
/ml ' .»
fmi •""

ND
ND
5
ND
ND
ND
5
7

ND
ND
5

ND
5
5
6
ND
ND
6
5

5
10
ND
6
ND
6
6
10

6
5
10
5
6
5
5
7
Cr



5
ND
5
ND
KD
5
ND
ND

ND
ND
5

5
ND
ND
5
ND
ND
ND
5

5
5
ND
ND
ND
9
9
5

5
5
ND
ND
ND
9
ND
5
Pb



ND
ND
ND
ND
ND
ND
ND
ND

ND
ND
ND

ND
100
50
ND
ND
ND
ND
ND

ND
120
ND
ND
ND
ND
ND
ND

70
100
50
ND
ND
50
ND
ND
         Year, month, day
         Not detectable
                                                                                                   (continued)

-------
TABLE 20.   (continued)
Date


760329 a
760520
760628
760714
760312
760921
761005
761122

760329
760520
760628
760714
760812
760921
761005
761122

760329
760520
760628
760714
760812
760921
761005
761122

760329
760520
760628
760714
760812
760921
761005
761122
Time


1125
1140
1010
1030
850
955
954
1005

1150
1155
1020
1050
910
1015
945
1025

1215
1220
1045
1115
925
.1040
916
1045

125
115
1125
1115
955
1110
1124
1115
Temp
"C

20.8
24.0
26.5
24.0
28.0
22.8
17.9
10.1

21.5
25.8
27.3
24.0
27.0
22.4
20.2
13.0

20.8
21.8
26.8
22.9
25.0
22.4
16.2
9.3

20.4
22.0
27.5
22.9
27.0
21.0
19.9
8.2
PH


7.0
7.0
7.7
7.1
7.0
7.1
7.4
7.2

7.1
7.0
8.0
7.8
7.6
7.5
7.8
7.8

7.0
7.1
7.3
7.6
7.3
7.2
7.5
7.3

6.5
6.9
7.3
7.6
7.0
7.1
7.0
7.1
NH+-M
jug/ml

NDb
ND
ND
ND
ND
ND
ND
ND

ND
ND
ND
ND
ND
ND
ND
ND

ND
ND
NO
ND
ND
ND
ND
ND

ND
ND
ND
ND
ND
ND
ND
ND
NO~-N
^Jg/ml

27.0
26.5
26.5
25.5
25.0
18.5
20.0
33.0

1.7
1.8
2.2
1.9
1.7
0.3
2.5
1.7

7.2
6.0
10.0
7.8
7.8
7.6
13.8
7.0

11.5
7.5
12.5
7.8
13.0
12.5
18.0
11.0
NOl-N Kjcldahl
N
ng/ml — • --- -----
LOCATION NO. 36
ND ND
5 ND
ND ND
10 ND
8 ND
350 0.7
33 0.6
SD ND
LOCATIOB KO. 37
ND ND
ND ND
ND ND
ND ND
ND ND
5 ND
8 ND
ND ND
LOCATION NO. 38
ND ND
ND ND
6 ND
ND ND
6 0.4
5 ND
11 ND
ND NO
LOCATION NO. 39
ND ND
ND ND
ND ND
KD ND
90 ND
240 0.7
22 0.6
119 SD
Dissolved
Ortho-P
— /jg/ml — —

ND
0.02
0.01
0.02
0.01
0.04
ND
ND

0.05
0.01
0.01
0.01
ND
0.01
0.01
0.01

0.02
0.02
0.01
0.01
0.02
HD
0.01
ND

ND
0.01
ND
0.01
0.01
ND
ND
ND
Total
P Zn


ND 140
0.03 75
0.01 49
0.02 168
0.01 98
0.08 61
0.05 49
ND 90

0.05 460
0.02 315
0.01 266
0.01 618
ND 300
0.01 750
0.02 653
0.01 520

0.02 720
0.03 820
0.09 544
0.01 2083
0.02 800
ND 2310
0.01 1870
0.02 5170

0.02 290
0.13 410
0.01- 6809
0.01 16940
0.02 9202
ND 19118
0.01 21175
ND 12100
Cu


44
74
41
86
30
26
22
22

15
ND
ND
11
ND
ND
ND
ND

31
ND
ND
11
ND
10
ND
ND

15
16
17
30
21
22
16
26
Ni
	 ng

15
45
22
25
20
44
29
23

15
ND
ND
ND
ND
ND
10
17

10
ND
10
ND
10
ND
17
14

55
70
40
56
51
88
63
54
Cd
/ml 	

5
5
8
7
4
9
6
9

ND
ND
ND
ND
ND
ND
ND
5

7
ND
5
ND
ND
ND
5
7

ND
10
12
12
10
18
13
16
Cr


5
5
9
ND
ND
9
5
5

ND
ND
ND
ND
ND
ND
ND
5

5
ND
ND
ND
ND
5
ND
ND

ND
7
15
11
10
15
14
8
Pb


70
100
50
80
ND
100
ND
ND

ND
80
ND
ND
ND
50
ND
ND

ND
80
ND
ND
ND
ND
ND
ND

130
100
100
105
100
175
100
100
  ? Year, month, day
   Not detectable
(continued)

-------
TABLE 20.   (continued)
Date

«
760329
760520
760628
760714
760812

760329
760520
760628
760714
760812
760921
761005
761122

760329
760520
760628
760714
760812
760921
761005
761122

760329
760520

760329
760520
Time


145
140
1130
1255
1010

215
200
1105
120
945
1050
1135
1100

240
215
1140
135
1020
1130
1100
1145

305
235

320
255
Temp
°C

20.0
21.0
21.4
22.8
25.0

21.5
21.6
20.8
21.0
21.5
22.4
20.9
10.8

20.8
20.7
21.0
20.5
21.0
23.0
21.9
12.8

22.7
22.3

20.5
21.0
pH


6.7
7.0
7.0
7.2
7.2

7.0
7.0
7.5
7.0
7.4
7.2
7.3
7.2

7.0
7.1
7.2
7.2
7.2
7.6
7.4
7.4

7.4
7.0

7.0
7.2
NrfJ-N
>ig/ml

NDb
ND
ND
ND
ND

ND
ND
ND
ND
ND
N£
ND
ND

ND
ND
ND
ND
ND
ND
ND
ND

ND
ND

ND
ND
NO'-N
^g/ml

10.0
15.5
15.0
13.5
10.5

24.0
27.0
29.5
27.5
24.5
25.5
37.5
24.0

18.0
24.0
24.0
26.0
27.5
31.5
42.0
27.0

2.1
2.3

46.5
25.0
Dissolved
NOl-N Kjeldahl Ortho-P
M


ND
9
ND
6
6

6
11
6
10
ND
8
ND
11

5
21
6
35
6
23
ND
ND

10
5

13
44
LOCATION NO. 40
ND
0.4
ND
ND
ND
LOCATION NO. 41
ND
ND
ND
ND
ND
ND
ND
ND
LOCATION NO. 42
ND
ND
ND
ND
ND
ND
ND
ND
LOCATION NO. 43
ND
ND
LOCATION NO. 44
ND
1.1
- /ig/ml --

ND
ND
ND
0.01
ND

ND
0.01
0.01
0.01
0,03
KD
0.01
ND

ND
0.02
0.01
0.01
0.01
ND
0.01
ND

0.02
0.01

' ND
0.01
Total
P
Zn
Cu
Hi
Cd
Cr
Fb


ND
ND
0.01
0.01
ND

ND
'0.03
0.01
0.01
0.03
ND
0.01
ND

ND
0.02
0.01
0.01
0.01
ND
0.01
ND

0.03
0.11

ND
0.01

175
100
59
19
140

230
275
50
213
99
145
273
370

210
121
88
283
107
412
383
610

390
515

255
160

11
13
25
47
12

19
29
28
37
12
16
ND
10

11
13
12
ND
12
10
ND
NS

84
135

19
13

45
52
45
383
42

25
32
22
25
24
17
26
23

15
15
15
16
20
17
20
17

10
25

25
88

11
7
13
10
7

KD
5
6
6
4
5
8
9

ND
7
6
5
4
ND
8
10

ND
ND

ND
10

ND
7
12
ND
KD

5
5
ND
ND
ND
8
ND
5

ND
5
9
ND
ND
8
ND
5

ND
ND

ND
5

100
100
70
80
50

ND
60
50
ND
ND
50
ND
250

ND
ND
50
ND
ND
50
ND
ND

ND
ND

ND
ND
    Tear, month, day
    Not detectable
                                                                                                (continued)

-------
         TABLE 20.    (continued)
Ln
00

Date


760329 a
760520
760628
760714
760812
760921
761005
761122

760714
760312
760921
761005
761122

760714
760812
760921
761005
761122

760312
760921
761005
761122

Mean
Minimum
Maximum
Standard

Time


335
325
1225
235
1005
100
1020
1245

155
1035
1145
1045
1205

215
1050
1230
1037
1230

745
820
830
845




deviation

Temp
°C

19.8
19.5
21.5
23.0
23.0
23.6
21.2
11.6

23.7
31.0
22.5
20.5
9.0

22.7
23.0
23.0
17.3
16.4

23.2
18.2
20.2
9.3






pH


7.1
7.1
7.6
7.3
7.3
7.4
7.5
7.3

7.1
7.5
7.5
7.5
7.3

7.2
7.3
7.3
7.4
7.1

7.4
7.2
7.4
7.2

7.3
6.5
8.0
0.2
^
NH^-N
^E/ml
t
NDb
ND
ND
ND
ND
ND
ND
ND

ND
ND
ND
ND
ND

ND
ND
ND
ND
ND

ND
ND
ND
ND

< 0.3
<0.3
<0.3
0

NO~-N
>.g/ml

39.0
49.5
54.0
61.0
47.0
57.0
67.0
45.0

1.9
1.8
2.6
3.2
2.3

36.5
45.0
38.0
57.0
36.0

60.0
48.5
84.0
49.0

22.0
0.3
84.0
18.4

NO~-N
ng/ml

ND
103
13
625
75
38
23
23

10
ND
ND
ND
ND

10
6
5
5
ND

6
13
ND
14
AI:
20
< 5
625
72

Kjeldalil
10

LOCATION NO. 45
ND
0.4
HD
ND
ND
KD
ND
0.5
LOCATION NO. 46
iro
HD
ND
KD
HD
LOCATION NO. 47
ND
ND
SD
ND
ND
LOCATICM NO. 48
:SD
tfD
HD
•JTO
L Samples CN=114)
< 0.30
< 0.30
1.10
0,19
Dissolved
Ortho-P


0.02
0.01
0.01
0.01
0.01
ND
0.01
ND

0.01
ND
0.01
0.01
ND

0.01
ND
ND
0.01
ND

ND
ND
0.01
ND

< 0.010
< 0.010
0.050
0.011
Total
P


0.02
0.03
0.01
0.11
0.05
ND
0.03
ND

0.02
ND
0.01
0.01
0.02

0.09
ND
ND
0.01
ND

ND
ND
0.01
0.02

0.018
< 0.010
0.130
0.024

Zn


650
4950
1848
1753
570
2101
1595
2772

5273
4125
4400
4735
5555'

7583
330
630
483
1000

350
1540
203
700

1572
19
21175
3497

Cu


11
ND
15
14
ND
ND
ND
ND

ND
ND
10
ND
ND

ND
ND
19
ND
ND

15
34
10
12

16
<10
135
19

Ni
— ng/ml

15
38
10
10
ND
10
14
11

10
ND
ND
10
ND

10
10
17
14
ND

10
10
14
14

25
< 10
383
38

Cd


ND
8
5
KD
ND
ND
6
7

5
4
ND
5
5

5
ND
ND
6
ND

ND
ND
5
5

5
< 4
18
4

Cr


ND
ND
ND
ND
ND
8
ND
5

ND
ND
ND
ND
5

ND
ND
6
ND
5

ND
5
ND
ND

< 5
< 5
15
4

Pb


ND
60
ND
ND
ND
ND
ND
ND

ND
ND
ND
ND
ND

ND
ND
ND
ND
ND

ND
ND
ND
ND

< 50
< 50
250
46
            Year, month, day
            Not detectable

-------
TABLE  21.  AVERAGE COD, IOD, TFR, AND TNFR ANALYSIS OF WATER
        SAMPLES FROM THE CONCHO RIVER, SEWAGE FARM LAGOONS, SEEPS
                 AND WELLS AND TOM GREEN COUNTY WELLS
Sample
Number

Location

BOD


Total
Filterable
COD Residue




Total
Nonf ilterable
Residue


SEWAGE FARM
1
2
3
4
5
6
7
8
9
10
12
13
14
16
18
19
20
21
22
Concho River - upstream
Concho River - downstream
Effluent Lagoon 1
Effluent Lagoon 4
Seepage Creek no. 1
Seepage Creek no. 2
Seepage Creek no. 3
Shallow Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Shallow Well
Shallow Well
Shallow Well
Shallow Well-
Sewage Treatment Plant
3.8
3.1
148.0
89.0
9.2
0.6
0.6
0.7
1.6
1.3
0.1
0.6
0.9
0.5
1.0
1.3
0.7
1.6
146.0
65
58
400
280
80
58
62
100
64
38
38
48
52
38
102
62
74
54
502
965
975
1298
1382
1630
1438
2200
1648
2245
1660
1691
1625
1763
1673
1803
1222
1933
2110
1528
30
25
968
1060
70
33
30
40
73
57
68
63
72
93
92
83
110
102
605
TOM GREEN COUNTY WELLS
31
33
34
35
36
37
38
39
41
42
45
46
>47
48
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well "
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
Domestic Well
1.6
1.2
1.3
0.5
0.8
1.8
1.5
0.1
2.3
0.1
2.1
1.1
0.6
1.5
46
6
15
11
<10
<10
<10
34
28
11
<10
<10
42
25
1528
1240
3200
2770
2445
485
808
5088
1603
1485
1025
470
1198
1035
78
105
100
115
130
100
152
43
<23
20
<21
20
21
30
                                  59

-------
NO™ nitrogen higher than 10 yg/ml may result in a high potential risk of
methemogloginemic. to bottle-fed infants.  Waters with a NO~-N concentration
over 1 yg/ml should not be used for infant feeding.  A limit of 0.02 yg/ml
of nonionized NH_ has been recommended as the water quality criteria for
freshwater aquatic life (EPA 1976).  A chemical equilibrium exists between
nonionized NH3 and NH4 ion.  At 20°C and a pH of 8.0 only 3.8 percent of the
nitrogen is present as nonionized NH_ in aqueous solution.  The other 96.2
percent is present as NH,  ions.  There is no water quality criteria for
N0~ or NH_ for livestock water supplies.  Campbell and others (NTAC, 1968)
have reported methemoglobinemia in cattle receiving water containing
2,790"yg/ml of N03-N.

     In ruminant animals,  NO,, may be reduced to tK^ by microflora in the
rumen.  Nitrite may then exert toxic effects on the animal.  High concen-
trations of NO- and/or NH, in waters may also stimulate growth of undesir-
able plants and decrease recreation uses.  The toxicity of ammonia is
dependant upon pH, temperature, the concentration of total ammonia
(NH- + NH,), and ionic strength.  The concentration of NH~ increases with
increasing temperature and decreases with increasing ionic strength.
Lykins and Smith (1976) reported that Missouri has the most stringent
standard of NH_ (0.1 yg/ml) for streams.  Nevada and California have the
lowest NO--N value (1.0 yg/ml), and Delaware has the lowest total nitrogen
value (3.0 yg/ml).

     Nitrogen entered the sewage treatment plant primarily as NH,-N (Table
11).  The 12 month mean was 16.3 yg NH,-N/ml but fluctuated between 12.0 and
29.2 yg/ml over the year.   In addition, an average of 5.9 yg/ml organic N
entered the treatment plant (difference between Kj eldahl-N and NH,-N).  A
trace of NOj-N and small but variable quantities of NO--N were present in
the incoming effluent.

     Ammonium nitrogen was the predominant form in the sewage effluent
lagoons (Tables 12 and 13) with mean values of 28.2 and 21.1 yg NH,-N/ml
found in effluent lagoons 1 and 4, respectively.  Total Kjeldahl-N also
tended to increase in concentration in the lagoons while NOl-N and NO--N
concentration remained fairly constant.

     Once sewage effluent water was applied to the soils, oxidation of
NH.-N occurred, and the nitrogen species found in seepage creeks was
N0~—N (Tables 14, 15, and 16).  The exception was seepage creek no. 1 where
significant quantities of NH.-N and Kjeldahl-N were measured.  Seepage
creek no.  1 originates within the boundary of the farm and flows through it.
Cattle drink and excrete into the stream.  On several occasions effluent
was detected flowing directly from the irrigation lines into seepage creek
no. 1.  These two factors probably largely account for the levels of NH.-N,
Kjeldahl-N and NO--N measured in the seepage creek no. 1 compared to
seepage creeks 2 and 3 (Tables 15 and 16).  The latter two seeps were
discharging from the bank of the Concho River.  They were fenced to exclude
cattle from drinking from them.  There was no possibility of surface flow
into these seeps, so all of the water had to pass through the soil profile.
Consequently,  NH.-N,  NO»-N and Kjeldahl-N were detected sporadically and in


                                      60

-------
small quantities.  Except  for  the high NO.--N concentration  (16.3 and 21.0
yg/ml) in seeps  2 and  3, the water was of good quality.

     The mean NO,-N value  of water from all three seepage creeks is higher
than 10 yg/ml.   The lower  the  water flow rate of  these three  seepage creeks,
the higher  the nitrate value found in the waters  (Tables 14,  15 and 16).
The average value of  183 ng NO^-N/ml in seepage creek no.  1 was much higher
than values in seepage creeks  no. 2 and 3.

     There  was a significant increase in the nitrate concentration of Concho
River waters below compared to that above the sewage farm (Tables 17 and  18).
The discharge of treated sewage effluent from the sewage farm into the
Concho River  (via seepage  and  some surface runoff) increased  the nitrate
concentration of river waters  from a mean value of 1.6 - 1.0  to 5.7 - 2.0
yg/ml NO~-N.  Because the  NO,-N value of downstream waters is less than
the standard of  10 yg/ml  (except on January 8, 1976,  a value  of 14.0 yg/ml
was observed), a minimal environmental impact is  expected. No significant
increase  in Kjeldahl-N, NH.-N, and NOl-N concentrations  was observed in the
Concho River below the sewage  farm.

     The  nitrate values of shallow ground wells and domestic  deep wells
in the sewage farm are 5,8 ± 5.2 and 15.7 ± 5.9 yg N03-N/ml,  respectively
 (Table 19). Both the NH.-N and Kjeldahl-N concentrations are below the
detection limit. Waters of high concentration of NOv-N  are not suitable
for drinking, but they do  make a good source of plant nutrients.   Most
deep well water  in Tom Green County has a high concentration  of NQ,-N.
At sampling locations no.  33,  45, 47, and 48, the concentration^
NO~-N is  higher  than  50 yg/ml  (Table 20).  Comparison of the  NO~-N
concentration of deep wells in the sewage farm and that  of Tom Green county
wells indicated  that  application of sewage wastewater to the  farm did not
increase  the NO~-N concentration.  The average NO.,-N concentration of deep
well water from  the  sewage farm was 15.7 (Table 19) while that of the deep
wells located  in Tom Green county was 22.0 (Table 20).

     In a study  to determine the source  of  N0_  in  ground water  using  N
isotope,  Kreitler (1975) found that the  major  source  of nitrate in ground
water in the San Angelo area is  from natural  soil  nitrate, which was derived
from the mineralization of  organic  nitrogen in the soil.   He  indicates  that
the Permian carbonate  aquifers and  the tertiary gravel aquifer  have no
downdip portion  so the entire  aquifer has been polluted.   He  concluded  that
the oxidation of part  of the humus  of semiarid  grassland soils  and  the
subsequent leaching of the  N0_ to the saturated zone  by extensive  terracing
in the 1950fs resulted in the  high  NO, concentration  in the ground waters
in this area.

Phosphorus

     Phosphorus is one of the  major  essential elements for plant growth.
In general, evidence indicates that  high phosphorus concentrations are
associated with accelerated  eutrophication  of waters, when other growth-
promoting factors are  present.   Phosphorus  is not  the sole cause of
eutrophication but there is  substantiating  evidence that frequently  it  is

                                      61

-------
the key element required by freshwater plants.  Because it is present in
the least amount relative to need, phosphorus is one of the most easily
controlled elements required for plant growth by man's activity.  Under
normal soil and water conditions plants take up phosphorus in the H^PO^
forms.  It is well-known that phosphorus concentrations critical to noxious
plant growth vary, and nuisance growths may result from a particular
phosphorus concentration in one geographical area but not in another.

     A level of 100 yg/1 total P has been suggested by MacKenthum (1973) as
the desired water quality criteria for the prevention of plant nuisance in
streams or other flowing waters not discharging directly into lakes or
impoundments.  At the time of the spring turnover total phosphate concentra-
tions in excess of 25 yg P/l may occasionally stimulate excessive or
nuisance growths of algae and other aquatic plants and interfere with water
treatment (EPA 1976).  Lykins and Smith (1976) reported that Hawaii has
the most stringent total phosphate phosphorus standard (20 yg P/l) for
streams.  At present no national criterion is presented for phosphate
phosphorus for the control of eutrophication (EPA 1976).

     The sewage farm is receiving about 67,900 kg total P/year from the city
of San Angelo if an average value of 0.219 m /sec of raw sewage is assumed.
The phosphorus is present as about 50 percent dissolved orthophosphate and
50 percent organic phosphorus (Table 11).   The average orthophosphate
concentration in 1976 was 5.08 yg/ml and the average total phosphorus
concentration was 9.83.  Considerable fluctuation in phosphate concentration
was apparent during the year ; however, the ratio of ortho- to total phospho-
rus remained about ^he same.  As the sewage effluent passed through the
lagoons the ortho- and total phosphorus concentration tended to increase
somewhat.  The average total phosphate concentration of effluent water at
the sewage treatment plant, lagoon no. 1 and lagoon no. 4 were 9.83 ± 3.71,
24.94 ± 8.02, and 18.45 ± 6.92 yg P/ml, respectively (Tables 11, 12 and 13).
The dissolved orthophosphate concentrations of the effluent at these three
locations were 5.08 ± 2.05, 11.35 ± 4.36,  and 7.58 ± 4.16 yg P/ml,

     Both the dissolved orthophosphate and total phosphorus of water
sampled at seepage creek no. 1 exceeded 100 yg P/l (Table 14).  From the
middle of February to early July, the total phosphorus concentration of
seepage creek no. 1 water often exceeded 1,000 yg P/l.   In the spring of the
year, total phosphorus concentrations of seepage creeks no. 2 and 3 were
higher than 25 yg P/l (Tables 15 and 16).   All three seepage creeks had
high total phosphorus concentration in the spring time and produced thick,
dense algae growth.  Algal growth interfered with water flow through weirs
installed at the three seepage creeks and  required frequent cleaning of the
weirs to obtain accurate data.

     Comparison of the dissolved orthophosphate and total phosphate between
domestical deep wells at the sewage farm and Tom Green County wells indica-
ted that no significant difference in phosphorus concentrations is found
(Table 19 and 20).   Apparently the application of sewage effluent to the
soil does not increase the dissolved orthophosphate or total phosphorus
concentrations in underground water.  Because no downdip portion existed in
the carbonate and gravel aquifers, water moves laterally rather than

                                      62

-------
vertically in the soil profile.  This water movement  apparently results  in
higher phosphorus concentrations in  surface flow waters  (Concho River and
seepage creeks), but not in deep well groundwater.  Control measures to
prevent the development of excessive vegetative growth within  the water ways
are necessary for the long-term operation of  the sewage  farm.

     A slight increase was found in  the  total phosphate  in the Concho River
waters below the San Angelo sewage farm  (Tables 17 and 18).  Total phos-
phorus concentration above the sewage farm was 0.060  yg  P/ml.  The concen-
tration below the farm was 0/078 yg  P/ml.  Dissolved  orthophosphate
increased from 0.017 to 0.019 yg P/ml.   Waters flowing from the seepage
creeks are probably representative of those flowing from the sewage farm
in terms of P concentration.  Total  phosphorus ranged from 0.03 yg P/ml
in seepage creek 3 to 0.79 yg P/ml in seepage creek 1.   Dissolved ortho-
phosphate ranged from 0.02 to 0.36 yg P/ml for the same  two sites.

Heavy Metals

     Excessive concentrations of heavy metals in water may constitute a
very serious form of pollution.  Heavy metals form relatively  stable
compounds and are not readily removed by natural processes.  A character-
istic feature of heavy metal pollution is its persistence in time as well
as in space for years after the pollution practices have ceased (NTAC 1968).
Living organisms can take up heavy metals from a solution against a concen-
tration gradient and concentrate them above that level normally found in
the medium.  The order of affinity of cations for plankton generally is
Zn>Pb>Cu>Mn>Ni>Cdi and for algae is  Pb>Mn>Zn>Cu,Cd>Co>Ni.  It  is clear that
the heavier metals tend to be more readily taken up than the lighter ones,
which have a higher degree of hydration.  The uptake  of  heavy metals by
living organisms is clearly linked with  metabolic activities within the
cell.  Many environmental factors (such  as temperature,  oxygen, light)
can affect the active uptake process of  heavy metals  by  living organisms.

     Heavy metals cause toxic action on  the growth and metabolism of an
organism even at very low concentration.  Many mechanisms of toxic action
have been observed; such as the poisoning of  enzyme systems, formation of
antimetabolites catalyzing the decomposition  of essential metabolites,
alteration of cell membrane permeability, and replacing  important elements
in the cell which affect their ability to function.   Living organisms
exhibit rather marked differences in tolerance to levels of heavy metals
in its environment.  One of the potential hazards in  applying  sewage
effluent to: land is the contamination of groundwater  and receiving streams
by heavy metals, and the subsequent  uptake of heavy metals by  aquatic
organisms.

     The data presented in Table 22  show the  water quality criteria for
heavy metals for domestic water supply and irrigation use.  The values of
the National Academy of Science (NAC 1973) refer to recommended maximum
concentrations of trace elements in  irrigation waters used for sensitive
crops on coarse-textured soils with  low  capacities to retain heavy metals.
Lykins and Smith (1976) listed states having  most stringent water quality
criteria of streams and the concentration of  each heavy  metal.

                                     63

-------
Table 22-  RECOMMENDED SURFACE AND IRRIGATION WATER QUALITY CRITERIA FOR
                              HEAVY METALS

                Stream        Domestic Water     Irrigation Water	
Elements   (Lykins and Smith)    Supplies     (NTAC 1968)   (NAS 1973)
                 1976           (EPA 1976)


Cd
Cr
Cu
Ni
Pb
Zn


0.
0.
0.
1.
0.
—


005
010
010
000
040



(Ohio)
(Ohio)
(Minn.)
(111.)
(Ohio)

1 I rt
— yg
0.
0.
i.
-
0.
5.
/ml

01
05
00

05
00


0.
5.
0.
0.
5.
5.


005
000
200
500
000
000


0.
0.
0.
0.
5.
2.


01
10
20
20
00
00
   Data in Tables 11, 12, and 13 show the heavy metals concentration of
incoming sewage effluent at the sewage treatment plant lagoon no. 1 and
lagoon no. 4, respectively.  The heavy metals concentration of lagoon no. 4
waters, which is irrigated directly to the land, is less than that
suggested by the National Technical Advisory Committee (1968) and by the
National Academy of Science (1973) for irrigation water (Table 13).  The
chromium concentration of water decreases from 0.272 ± 0.169-yg/ml at the
sewage treatment plant to 0.064 ± 0.021 yg/ml at lagoon no. 4;  whereas no
significant change was observed in the Cd, Cu, Ni, Pb, and Zn concentra-
tions.  The heavy metal concentration of three seepage creeks,  the shallow
ground wells, and the deep wells for domestic use located within the
sewage farm are below the water quality criteria for either domestic water
supply or irrigation use (Tables 14, 15, 16 and 19).  Most Tom Green
County deep well waters have low concentrations of heavy metals for
drinking and irrigation purposes (Table 20).  Waters sampled at Tom Green
County well locations no. 38, 39, 45, 46, and 47 have high zinc concen-
trations which exceeded the water quality standard for either domestical
drinking or irrigation.  The zinc concentration of water at sampling site
no. 39 increased from 0-29 yg Zn/ml in March to 21.18 yg Zn/ml in October,
then dropped to 12.10 yg Zn/ml in November in 1976.  It is not clear what
causes this high zinc concentration in these waters.  Corrosion and con-
tamination of the water by galvanized well pipe is probably the main
factor.

   No significant difference in Cu, Ni, Cd, Cr, and Pb concentrations in
Concho River waters above and below the sewage farm was found (Tables 17
and 18).  The average zinc concentration (0.123 ± 0.101 yg/ml)  of Concho
River water below the sewage farm is higher than that (0.050 ± 0.065 yg/ml)
above the sewage farm.  The concentrations of heavy metals in the Concho
River above and below the sewage farm do not exceed the water quality
criteria recommended by the U. S. Environmental Protection Agency  (1976)
for domestic water supplies.  It is clear that the discharge of sewage
farm water into the Concho River does not change the water quality of  the
Concho River with respect to the heavy metals.

                                     64

-------
BOD and COD

     The biochemical oxygen demand  (BOD)  indicates  the relative oxygen re-
quirements of wastewaters, effluents, and polluted waters.  It also can be
used in evaluating the efficiency  (BOD  removal) of wastewater treatment
systems.  The measurement of  chemical oxygen  demand  (COD) provides the
oxygen equivalent of that portion  of the  organic matter  in water  that is
susceptible to oxidation by potassium dichromate.  With  wastewater con-
taining toxic substances, the COD  test  may  be the only measurement for
evaluating the organic load.  The  BOD and COD values of  water at  the sewage
farm and Tom Green County are listed in Table 21.

     The BOD values of incoming  sewage  wastewater at the sewage treatment
plant and effluent lagoon no. 1  are almost  the same, 146 and 148  yg/ml,
respectively.  It drops to 89 yg/ml at  effluent lagoon no. 4.  The COD
value of incoming sewage wastewater is  502  yg/ml.   It decreases to 400 and
280 yg/ml at the effluent lagoon no. 1  and  4,  respectively.  There was
essentially no difference between  the BOD and COD values of Concho River
waters above and below the sewage  farm.   Therefore, it can be inferred that
sewage farm operation has no  effect on  the  BOD arid  COD values of  the Concho
River on the dates measured.  Considerable  reduction in  BOD and COD values
are apparent between the applied wastewater and the three seepage creek
waters.  The BOD values of well  waters  in the sewage farm are similar to
those of well waters in Tom Green  County.   However, well waters in the
sewage farm generally had higher values of  COD than domestic wells in
Tom Green County.  Infiltration  of  applied  sewage wastewater into and
through the sewage farm soils decrease  the  BOD and  COD values of  the
wastewater.  The soils act as a  living  filter.  In  the United States,
Nevada has the most stringent BOD  criteria  level of 1.0  yg/ml for streams
(Lykins and Smith 1976).  The highest BOD values for deep well waters in
this study were 2.1 and 2.3 yg/ml  and came  from domestic wells located in
Tom Green County.

Total Filterable Residue  (TFR) and Total Ndnfiltefable Residue (TNFR)

     Waters yielding a high value  of residue  are generally inferior with
respect to palatability, or they may induce an unfavorable physiological
reaction in the transient consumer. A  limit  of 500 yg/ml total filterable
residue (TFR) is the permissible criteria^,  and a limit of less than 200
yg/ml is the desirable level  for public drinking water  (NTAC 1968;.  The
value of 500 yg/ml TFR is also the recommended level for farmstead use.
The state of Illinois has the most stringent  total  nonfilterable  residue
(TNFR) level of 5.0 yg/mlj whereas  Nevada has the most stringest  TFR level
of 60 yg/ml (Lykins and Smith 1976).  High  TNFR values are objectionable
because of physiological effects,  abnormal  taste, or economic effect
(corrosion damage in water systems by high  concentrations of sulfates
and chlorides).

     The data presented in Table 21 show  TFR  and TNFR values of waters
from the sewage farm and Tom  Green County wells.  All waters in the
sewage farm and most well waters in Tom Green County have values  of TFR
greater than 1000 yg/ml.  The Concho River  waters above  and below the

                                     65

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sewage farm have values of 965 and 975 yg/ml, respectively.  The deep well
located near the treatment plant had the highest value  (2245 yg/ml) of TFR
in the sewage farm while water sampling site no. 39, located in the city of
San Angelo, has the highest value (5088 yg/ml) of TFR in the Tom Green
County wells sampled.  Comparison of TFR and TNFR values between sewage
farm well waters and those from Tom Green County wells  indicates that
application of sewage wastewater to the soil does not increase the TFR and
TNFR values in ground waters in the sewage farm.  The high values of TFR in
Tom Green County are probably the result of dissolution of salts from
underground rocks which are sedimentary origin.  The principal ions in
the well waters are calcium, bicarbonate, and sulfate,  and, in general, the
water is excessively hard (Willis, 1954).

     There was no apparent difference in TFR and TNFR in Concho River waters
sampled upstream or downstream from the sewage farm (Table 21).

Statistical Relationships between Water Parameters

     Correlation coefficients between the air and soil  temperatures and
nutrient concentrations of three seepage creeks are calculated and listed
in Table 23.  At seepage creeks no. 1 and 2 the zinc concentration is
linearly correlated with the air and soil temperatures.  The location of
the temperature measuring equipment was at the fenced bermudagrass area,
adjacent to seepage creek no. 1.  No linear correlation is observed between
the zinc concentration of seepage creek no. 3 and the measured air and
soil temperatures.  Waters sampled at seepage creek no. 3 are located in a
dense mesquite tree area along the Concho River bank.   The temperature of
the water at seepage creek no. 3 was usually cooler than that of seepage
creeks no. 1 and 2 at the same time of the day.  The air and soil tempera-
tures measured at the fenced bermudagrass area are not representative of
the air and soil temperature of seepage creek no. 3.  A highly significant
linear correlation existed between the NO^-N concentration of seepage creek
no. 3 and the soil temperature at 10 and 50 cm depth.

     Linear correlation coefficients between the nutrient concentrations
and the temperature of water at the sampling location at the sewage farm
were calculated.  Furthermore, linear correlation coefficients between
nutrient species were also calculated.  Only those linear correlation
coefficients with an absolute value greater than 0.600 are listed in Table
24.  Highly significant linear correlation coefficients between total
phosphorus and dissolved orthophosphorus are found at the three seepage
creeks and most of the shallow and deep wells at the sewage farm.  Water
temperature and nitrate concentration of Concho River waters were negatively
correlated.  Water temperature and dissolved orthophosphate concentration
of incoming sewage effluent at the sewage treatment plant, lagoon no. 1,
and lagoon no. 4 are also negatively correlated.  At the sewage treatment
plant the water temperature is negatively correlated with the total
phosphorus, total Kjeldahl nitrogen, NH.-N, and dissolved orthophosphorus
concentrations.  Highly significant positive linear correlation coefficients
between total phosphorus, dissolved orthophosphorus, NH.-N and total
Kjeldahl nitrogen are found in water samples taken at the sewage treatment
                                      66

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TABLE 23.  CORRELATION COEFFICIENTS AND SIGNIFICANT PROBABILITY LEVEL OF DAILY AIR AND SOIL
            TEMPERATURES VS. NUTRIENT CONCENTRATIONS OF THREE SEEPAGE CREEK WATERS
Temperature

Air
Soil at 10 cm
Soil at 40 cm
<-»
	 yg/n
-0.002
0.992
-0.008
0.967
-0.050
0.798
N03~-N
,1

-0.251
0.188
0.086
0.656
0.151
0.434
N02~-N
ng/ml
See
0.188
0.328
0.225
0.241
0.194
0.314
Kjl-N
Ortho-P
Total-P
Zn
Cu
Ni

page creek
0.081
0.685
0.045
0.820
0.012
0.952
Seepage creek
Air
Soil at 10 cm
Soil at 40 cm
0.000
1.000
0.000
1.000
0.000
1.000
-0.227
0.236
-0.100
0.605
-0.011
0.095
0.223
0.245
0.378
0.043
0.385
0.039
0.170
0.378
0.269
0.158
0.302
0.111
no. 1
0.199
0.300
0.135
0.485
0.120
0.534
no. 2
-0.357
0.058
-0.250
0.191
-0.235
0.219

0.287
0.132
0.191
0.322
0.140
0.468

-0.404
0.030
-0.315
0.096
-0.315
0.096
0.540
0.003
0.577
0.001
0.583
0.001

0.667
0.000
0.675
0.000
0.693
0.000
-0.251
) 0.189
-0.037
0.848
0.057
0.770

0.031
0.874
0.125
0.520
0.181
0.348
0.210
0.275
0.164
0.397
0.141
0.467

0.150
0.436
0.171
0.376
0.176
0.361
                                                                                 (continued)

-------
        TABLE 23. (continued)
ON
QO
Temperature
NH4+-N
N03~-N
	 yg/ml 	
Air
Soil at 10 cm
Soil at 40 cm
0.000
1.000
0.000
1.000
0.000
1.000
-0.227
0.236
-0.100
0.605
-0.011
0.095
N02~-N
ng/ml
See
0.223
0.245
0.378
0.043
0.385
0.039
Kjl-N

Ortho-P

page creek no. 3
0.170
0.378
0.269
0.158
0.302
0.111
-0.357
0.058
-0.250
0.191
-0.235
0.219
Total-P


-0.404
0.030
-0.315
0.096
-0.315
0.096
Zn


0.667
0.000
0.675
0.000
0.693
0.000
Cu


0.031
0.874
0.125
0.520
0.181
0.348
Ni


0.150
0.436
0.171
0.376
0.176
0.361

-------
TABLE 24.  CORRELATION COEFFICIENTS OF TEMPERATURE AND NUTRIENT CONCENTRATIONS
           VS. NUTRIENT CONCENTRATIONS OF WATERS IN THE SEWAGE FARM a

Sampling Sampling
Location Location
Number Correlation Coefficient Number Correlation Coefficient
1
2


3


4




5







6

7
9
10
NO~-N
Zn
N02-N
NO^-N
NH4-N
Temp
NO^-N
Ortho-P
NHJ-N
NO^-N
Ortho-P

NH4-N
Total-P
Ortho-P
Total-P
total-P
Temp
Ortho-P

Total-P

Total-P
Total-P
Total-P
vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.

vs.
vs.
vs.
vs.
vs.
vs.
vs.

vs.

vs.
vs.
vs.
Temp
Temp
Temp
Temp
Ortho-P
Ortho-P
Ortho-P
NH4-N
Temp
NOji-N
Temp

Kjl-N
Kjl-N
Kjl-N
Ortho-P
NHj-N
Zn
NHj-N

Ortho-P

Ortho-P
Ortho-P
Ortho-P
-0
0
0
-0
0
-0
-0
0
-0
0
-0

0
0
0
0
0
0
0

0

0
0
0
.800 12
.754 13
.624
.601 14
.845 17
.718
.616
.875 18
.777
.743 19
.645
20
.896
.800 22
.796
.778
.730
.683
.657

.852

.774
.755
.784
Total-P
Total-P

Total-P
Kjl-N
N02-N
Total-P
Total-P

Total-P

Total-P

Total-P
Ortho-P
Total-P
Kjl-N
Total-P
Ortho-P
Total-P
Kjl-N
NH^-N
Ortho-P


vs.
vs.

vs.
vs.
vs.
vs.
vs.

vs.

vs.

vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.
vs.


Ortho-P
Ortho-P

Ortho-P
NH4-N
Temp
Ortho-P
Ortho-P

Ortho-P

Ortho-P

Ortho-P
NH4-N
NH^-N
NH-4--N
Kjl-N
Kjl-N
Temp
Temp
Temp
Temp


0.
0.

0.
0.
0.
0.
0.

0.

0.

0.
0.
0.
0.
0.
0.
-0.
-0.
-0.
-0.


857
607

661
933
683
663
797

972

671

924
917
898
896
882
866
772
735
677
644


 a Correlation coefficients of NH4-N,  N03-N,  N02-N,  Kjl-N,  Total-P, Ortho-P
   Zn,  Cu,  and Ni versus NO^-N, NO^-N,  Kjl-N, Ortho-P,  Total-P,  and
   temperature were calculated for all water  sampling locations  at the
   sewage farm.   Any coefficient not listed in this  table has  an absolute
   value of less than 0.600.
   Significant  at  1% level
                                      69

-------
plant and seepage creek no.  1.  It is apparent that water  temperature
influences the concentration of certain nutrients in waters.
PLANT ANALYSIS AND FIELD PLOT EXPERIMENT

     One of the major concerns of land disposal of sewage wastes is  the
cycling of chemical elements in sewage treated soils by the soil-plant
system.  Sixteen elements have been shown to be essential for plant  growth.
Four other elements are essential for some plants.  Some elements may have
little or no effect on plant growth, whereas others may be toxic.  Many
factors affect the toxicity of an element to a plant including plant
species and variety, plant growth stage, soil solution concentration, soil
availability and competition^ and balance of other elements.  Generally, the
concentration of a chemical element in plant tissue increases as the
concentration and availability of that element in the soil increases if
other growth factors are the same.  Plant species, even varieties within
species, exhibit natural variability and vary markedly in their tolerance
to concentrations of specific elements.  Balance and competition of other
elements are also important in determining plant requirements and toxicity
for a particular element.  Whenever the concentration of a given element in
a plant or the amount of that element supplied by the soil exceeds that
needed for sufficient and optimum growth, accumulation or toxicity of that
element may occur.  The accumulation of toxic elements will in turn deter-
mine the long-term loading limits of sewage waste disposal on a land.  One
of the objectives of this study is to examine the effect of long-term
sewage wastewater irrigation on plant growth and crop quality.

     The data in Table .   show the chemical composition and yield of four
forage crops grown at tJ    ^ewage farm.  Each set of data comes from a
sample taken from a dif    nt part of the field.  Coastal bermudagrass was
harvested on two diffen    dates.  The nitrogen concentration of individual
plant tissues ranged fr'    .80 percent for Coastal bermudagrass on the first
cutting to 1.62 percent    the second cutting.  This corresponds to  17.50
and 10.13 percent crude protein in plants, respectively.  The phosphorus
concentrations of plant tissue range from an average of 0.199 percent for
the first cutting of Coastal bermudagrass to an average of 0.343 percent
for forage sorghum.  The yield of the second harvest of Coastal bermuda-*-
grass was the highest (6000 Kg/ha),  whereas the yield of NK37 bermudagrass
was the lowest (2146 Kg/ha).   Generally, the nutrient concentration in .the
first cutting of Coastal bermudagrass is higher than that of the second
cutting.

     Both zinc and copper are required for normal plant growth.   The con-
centration of copper in plant tissues varies from 3.8 Ug/g for the second
cutting of the Coastal bermudagrass to 9.1 Ug/g for forage sorghum.  Allaway
(1968)  indicated that the normal range of copper in plants is 4-15 Ug/g and
the toxicity level is 20 ug Gu/g.   All the plants1  tissues contain less than
200 Ug/g  Zn,  the toxicity level suggested by Allaway (1968).  Melstead (1973)
considered 300 Ug/g Zn in plant tissues as the maximum concentration value
for plants.   Cadmium,  chromium,  and nickel are not considered as essential
for plant growth.   Melstead (1973)  suggested that the maximum .concentrations


                                     70

-------
TABLE 25.  NUTRIENT COMPOSITION AND YIELD FOR SINGLE CUTTINGS OF NK37 BERMUDAGRASS,
 FORAGE SORGHUM, RESCUEGRASS AND COASTAL BERMUDAGRASS AT THE SAN ANGELO SEWAGE FARM
Sampling
Crop Acreages Data
ha
KK37 bermudagrass 14.2 750902


Forage Sorghum 75.9 750902


Pvescuegrass 40.5 760513


Coastal 127.5 760728
beraiudagrass

Coastal 127.5 760810
bermudagrass

Cd


0.2
0.3
0.5
0.2
0.3
0.2
0.4
0.3
0,3
0.4
0.3
0.3
0.4
0.5
0.3
Cr


*
0.5
1.2
0.8
0.8
0.8

-------
of Cd and Cr in plants are 3 and 2 yg/g, respectively.  None of the plant
tissue taken from the sewage farm had concentrations of Cd and Cr greater
than that.  The concentration of Ni of plant tissues was far less than  50
yg/g, the toxicity level suggested by Allaway  (1968).

     Several studies have shown the accumulation of trace elements in plants
grown in sewage waste treated soils.  In his investigation of trace element
concentrations of plants grown on soil treated with sewage sludge over  a
period of 19 years, Le Riche (1968) found no adverse effects on crop yields,
but the plants accumulated abnormally large amounts of certain trace
elements.  John et al. (1972) investigated the factors affecting plant  up-
take and phytotoxicity of Cd added to soils.  They found that Cd may reaaily
be taken up from the soil and may result in potentially hazardous accumula-
tion of Cd in plants.  Page et al. (1972) reported that plant leaves were
capable of accumulating excessive amounts of Cd when the culture solution
concentration was a few tenths of 1 ppm.  Day and Kirkpatrick (1973) showed
that wastewater generally produced equal or somewhat higher yields of grain
or forage than well water with N, P, and K added equal to the N, P, and K of
the wastewater.  Hodgson (1970) found that the ratio of cadmium concentration
in plants to that in the corresponding soil was about 10 to 1.  He also
showed that the ratio of total chromium in plants to that in soil was 0.02.
Allaway (1968) indicated that plants may grow normally but contain levels of
Cd, Mo, Ni, and Se which are toxic to animals.  Plants may exclude As, Be, I,
F, Zn, Ni, and others by only minimal adsorption of these trace elements
from soils.

     Sewage wastewaters contain certain amounts of nitrogen, phosphate,
carbon, and other chemical species as well as heavy metals.  The inter-
actions between the heavy metals and all other chemical species play an
important role in the fate of applied chemical elements in the soil.  Agboala
and Corey (1973) stated that soil phosphate level is one of four major
factors which affect the availability of zinc to plants.  Giordano and
Mortvedt (1976) found that application of nitrogen fertilizer did not affect
the downward movement of heavy metals in soil but enhanced uptake of these
metals by plants.  Miller et al. (1976) investigated the relationship
between uptake of cadmium by soybeans and available soil phosphorus and
found cadmium accumulation increased with increasingly available soil
phosphorus.  Williams and David (1976) reported that in a field experiment
uptake by wheat of both native soil cadmium and cadmium residue from super-
phosphate was substantially increased by the application of ammonium nitrate.
They also showed that at high levels of application, phosphate significantly
affected the uptake of cadmium by plants.  Caro (1974) showed the normal
range of concentration of several trace elements formed in average super-
phosphate as follows:  Cd, 50-170 ppm; Cr, 66-243 ppm; Cu, 4-79 ppm; Ni, 7-32
ppm; and Zn, 50-1430 ppm.  Ozanne et al. (1976) found that phosphatic fer-
tilizers contain appreciable amounts of zinc and play an important part in
meeting the zinc requirements of plants on some Zn-deficient Australian
soils.

     The effects of nitrogen and phosphate fertilization and cutting on the
chemical composition and yields of Coastal bermudagrass grown in the
fenced plot area are listed in Table 26.  The date of the first cutting of


                                      72

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    TABLE 26.  YIELD AND NUTRIENT COMPOSITION OF COASTAL BERMUDAGRASS AS INFLUENCED BY N AND P FERTILIZATION
CO
PloC
Mo.
Bate of
fertilizer
Cutting
H
S
CH
Cr
PUnt
Cu
Composition
HI
Zn
N
	 Xg/lia 	 	 PK'g 	 	 	
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
f ^
if
18
19
20
21
22
23
24
25
26
27
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2.
2
2
2
2
224.4
224.4
0.0
0.0
112.2
112.2
112.2
0.0
224.4
224.4
0.0
112.2
0.0
0.0
224.4
112.2
*t"JL A
£ ***. *t
112.2
224.4
0.0
0.0
224.4
112.2
0.0
112.2
224.4
112.2
224.4
224.4
0.0
0.0
112.2
112.2
112.2
0.0
224.4
224.4
0.0
112.2
0.0
0.0
224.4
112.2
224.4
112.2
224.4
0.0
0.0
22'.. 4
112.2
0.0
112.2
224.4
112.2
112.2
56.1
0.0
56.1
112.2
56.1
0.0
112.2
o o
112.2
112.2
112.2
56.1
0.0
0.0
56.1
56 1
JO . 1
0.0
56.1
0.0
112.2
0.0
0.0
56.1
112.2
112.2
56.1
112.2
56.1
0.0
56.1
112.2
56.1
0.0
112.2
0.0
112.2
112.2
112.2
56.1
0.0
0.0
56.1
56.1
0.0
56.1
0.0
112.2
0.0
0.0
56.1
112.2
112.2
56.1
0.3
0.4
0.4
0.2
0.3
0.3
0.3
0.4
0.4
0.3
0.3
0.2
0.3
0.3
0.2
0.3
0.2
0.2
0.2
0.3
0.3
0.3
0.2
0.3
0.3
0.3
0.4
0.1
0.1
0.1
0.2
0.1
0.
0.
0,
0.
0.
0.
0.
0.
0.
0.2
0.1
0.0
0.2
0.2
0.2
0.1
0.4
0.2
0.5
0.1
0.1
0.1
0.7
0.3
0.4
0.7
0.6
0.6
0.6
0.7
0.7
0.4
0.3
1.0
1.0
1.0
1.0
1.3
1.8
1.8
1.8
1.6
1.8
1.8
1.6
1.2
1.4
0.7
0.8
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.3
0.3
0.5
0.8
0.5
0.5
0.1
0.1
0.5
0.3
0.8
6.7
5.6
5.6
5.6
6.4
7.0
6.0
6.4
6.7
5.4
10.0
5.0
6.7
5.6
5.0
5.4
5.6
6.0
7.0
5.6
6.0
6.4
6.8
5.4
8,2
8.2.
7.0
6.7
6.7
6.3
6.3
5.7
5.9
6.5
5.9
6.1
5.9
5.9
7.1
5.7
5.7
5.7
5.7
7.9
8.9
5.7
7.6
5.7
5.7
7.1
7.1
7.5
7.6
2.8
2.0
1.2
1.2
1.6
1.2
2.0
1.2
2.0
1.2
1.2
1.2
2.4
2.0
2.4
1.2
1.2
1.2
1.2
4.0
3.2
2.8
3.0
2.8
4.0
2.8
3.0
3.4
3.7
3.4
2.8
2.8
4.0
3.1
3.1
3.1
2.5
3.7
3.4
3.1
4.0
3.1
4.0
3.1
3.1
3.1
4.0
4.3
3.1
4.0
3.4
4.0
3.4
35
28
30
26
31
35
31
27
40
36
35
32
37
21
37
30
30
28
33
28
26
24
25
21
33
33
60
40
37
34
56
38
38
48
62
44
51
40
44
40
40
38
36
48
47
37
33
33
86
93
45
42
66
2.690
2.834
2.683
2.583
2.753
2.695
2.650
2.624
2.352
2.578
2.476
2.588
3.003
2.655
2.683
2.593
2£ 1 •)
• Ol*
2.767
2.841
2.869
2.650
2.593
2.514
2.962
2.576
2.979
2.151
2.451
2.051
2.354
2.236
1.995
1.883
1.976
2.054
1.941
1.952
2.113
2.057
2.067
2.531
2.526
2.464
2.585
2.577
2.612
2.365
2.378
2.665
2.337
2.494
2.553
2.467
2.644
P
1, 	
0.278
0.278
0.243
0.256
0.270
0.316
0.313
0.266
0.313
0.300
0.323
0.280
0.233
0.266
0.280
0.260
0.2SO
0.280
0.300
0.266
0.260
0.260
0.300
0.266
0.350
0.330
0.286
0.256
0.264
0.233
0.233
0.233
0.249
0.276
0.236
0.256
0.263
0.256
0.268
0.279
0.276
0.276
0.286
0.316
0.303
0.276
0.286
0.268
0.279
0.276
0.299
0.286
0.303
Protein
*
16.81
17.71
16.77
16.14
17.20
16.84
16.56
16.40
14.70
16.11
15.47
16.17
13.77
16.59
16.77
16.20
1 f -If
lu . $£.
17.29
17.75
18.05
16.56
16.20
15.71
18.51
16.10
18.62
13.44
15.32
12.82
14,71
13.98
12.47
11.80
12.35
12.84
12.13
12.20
13.20
12.85
12.92
15.82
15.79
15.40
16.15
16.10
16.32
14.78
14.86
16.66
14.31
15.58
15.95
15.42
16.52
Yield
Kg/h«
9913
14085
10970
12202
11268
15656
12907
15263
14139
15087
11647
12893
12040
9101
8S57
10401
10266
10130
11078
7855
10469
7882
11444
9792
12717
7787
5647
6121
5959
6677
5893
8694
7841
7286
7950
8193
6365
7367
7625
5638
2532
4103
3724
4239
4117
5146
4469
6162
4753
5078
3914
4347
3927

-------
Coastal bermudagrass was July 20, 1976 and the second cutting September
15, 1976.  The nitrogen concentration of plant tissues of Coastal bermuda-
grass ranged from 1.89 to 2.98 percent.  This corresponds to 11.8 and  18.6
percent crude protein in plants.  The phosphorus concentration of Coastal
bermudagrass varied from 0.233 to 0.350 percent.  Fertilization with
nitrogen and/or phosphate has no significant effect on the chemical compo-
sition and yield of Coastal bermudagrass grown in the sewage farm plots
(Table 27).

      San Angelo  city  employees  have annually applied  40,000  pounds  of
N  (as NILNO-) to the forage crops.  In view of the total nitrogen in the
sewage effluent and high fertility of the soil from past effluent appli-
cations the practice of applying additional fertilizer N would seem highly
questionable.  Applications as high as 224 kg N/ha and 112 kg P-O^/ha did
not significantly increase yield or nutrient composition of Coastal
bermudagrass.

     The concentrations of heavy metals (Cd, Cr, Cu, Ni, and Zn) in plants
are all in the normal range reported by Allaway (1968) as discussed before.
No evidence of accumulation of heavy metals in Coastal bermudagrass is
observed  (Table 26).  There are highly significant (at 1 percent level)
correlation coefficients between cutting and Cd, Cr, Ni, Zn,  and N
concentrations and between cutting and yields (Table 27).  Cutting has a
significant effect on the Cd, Cr, Ni, Zn, and N concentrations, and yields
of Coastal bermudagrass (Table 27).  Application of average amounts of N
and P fertilizers on the sewage farm do not increase the forage yield.
There was no evidence to show that accumulation of heavy metals in plants
was related to impurities in the N and P fertilizers.
SOIL ANALYSIS

Introduction

     Soils on the San Angelo sewage farm are from three major soil series
(Angelo, Mereta, and Rioconcho).  These soils belong to the great group
Calciustolls which are characterized by a distinct lime (CaCO~) accumu-
lation within 1.3 m of the soil surface.  Appreciable amounts of mont-
morillonite and mixed clay minerals are found in the soils.  These red to
brown soils, formed in outwash, clayey to silty red bed, or over limestone
in the Rolling Plains in Central Texas, are classified as Mollisols
(suborder).

     The Angelo soil is well drained, and surface runoff is slow.  This
calcareous soil has a distinct zone of calcium carbonate accumulation at
a depth of about 70 cm.  The permeability is moderately slow, and the
available water capacity is high.  The soil profile can be distinguished
by the following horizons:  Ap, B21, B22, B23  , and C.  The Angelo
solum thickness is 150 to 300 cm.  Angelo soils are deeper than the Mereta
soils but lack an indurated zone of calcium carbonate accumulation.  These
are productive soils when used for crops and as rangeland.
                                     74

-------
                   TABLE 27.  STATISTICAL ANALYSIS SHOWING THE EFFECT OF N AND P FERTILIZATION

                 ON PLANT COMPOSITION AND YIELDS OF COASTAL BERMUDAGRASS GROWN IN THE SEWAGE  FARM
-•j
Ui
Effect

N fertilizer
P fertilizer
Cutting
N
A.
0.0236
0.865
-0.0944
0.497
—0.6293
0.000
P
r
Correlation
0.1556
0.261
0.0724
0.602
-0.2215
0.107
Cd
coefficients
0.0198
0.886
0.15S5
0.252
-0.5985
0.000
Cr
Cu
Ni
Zn
Yield
and significant probability level
0.0048
0.972
-0.1151
0.407
—0.5561
0.000
B. Values of F
Cutting
N fertilizer
P fertilizer
N and P fertilizers
Cutting and N
Cutting and P
Cutting and N and P
29.47*
0.68
0.53
0.63
0.40
0.49
0.55
2.39
0.67
0.20
1.19
0.28
0.18
0.72
25.35a
o.::4
1.19
O.L9
0.30
0.52
0.80
18.313
0.00
0.55
0.18
0.03
0.70
0.40
-0.0934
0.501
0.2325
0.090
0.1173
0.398
test
0.67
0.33
1.49
0.77
0.36
0.22
1.04
0.0985
0.478.
0.0022
0.986
0.7019
0.000

43.82a
0.85
0.24
0.97
0.02
0.61
0.45
0.0182
0.895
-0.0066
0.961
0.6041
0.000

25.34a
0.12
0.01
0.81
0.58
0.06
0.82
-0.0248
0.858
0.1002
0.470
-0.8143
0.000

77.S43
0.08
0.60
0.36
0.19
0.14
0.01
         significant at 1% level

-------
     The Mereta soils are well drained, and the surface runoff is  slow.   The
available water capacity is low and limits vegetative production.  They have
indurated caliche at less than 50 cm depth.  The surface layer is dark
brown or dark grayish brown clay loam about 30.48 cm thick.  The Mereta
series is located in the level to gently sloping parts of the outwash
plains of Tom Green County.  The soil profile can be distinguished by the
following horizons:   Ap, A12,  A13,  CI^san,  C2Ca, and C3.  The indurated
caliche is located in the C,    horizon (45 to 54 cm depth). . The indurated '
caliche horizon may have roots and a little soil in the cracks and between
plates.  It contains a few to 10 percent by volume of caliche fragments
less than 2.54 cm in diameter.  The Mereta solum thickness ranges from
35 to 50 cm.  Land leveling, terracing, and depth of plowing are limited
by the shallow depth of hard caliche.

     The Rioconcho soils are well drained, and surface runoff is slow.  The
permeability is moderately slow, and the available water capacity is high.
The Rioconcho series is located in the alluvial flood plain of streams
draining limestone areas.  The surface layer is dark grayish brown clay
loam about 95 cm thick.  The thickness of Rioconcho soils over gravel, sand,
or limestone ranges from 180 to 600 cm.  Only three soil horizons (All,
A12, and C) can be distinguished in the Rioconcho soil profile.   The
thickness of the Dark A horizon ranges from 51 to 127 cm.   These soils are
well suited for cropland and are frequently irrigated.  Flooding occurs
once in 3 to 20 years but seldom lasts for more than 1 day.

Initial Characterization of Physical and Chemical Properties

     Initially a total of 180 surface soil samples were analyzed for organic
matter, carbonate, cation exchange capacity, total phosphorus, water
soluble phosphate, total nitrogen,  and IN HC1 extractable Cd, Cr, Cu, Ni,
Pb, and Zn.  These surface samples were taken from the 3 soil series
located on the sewage farm.

     The chemical and physical properties of these three sewage farm soil
series compiled from samples taken to a depth of 30 cm are listed in
Table 28.

Texture

     Rioconcho and Angelo soils had more than 38 percent clay in the upper
40 cm of the soil profile.  Mereta soil had a clay content ranging from
27 to 35 percent.  Angelo and Mereta soils are classified as clay loams
and Rioconcho as clay.

Soil pH

     The pH value of all three soils in 1:1 soil-water solution is greater
than 7.6.
                                     76

-------
TABLE 28.  CHEMICAL AND PHYSICAL PROPERTIES OF THE TOP 40 CM OF THREE MAJOR SOIL SERIES
                          FROM THE SAN ANGELO SEWAGE FARM

Soil
Series Depth
en
Aloconcho 0-5
5-10
10-20
20-30
30-40
Angelo 0-5
5-10
10-20
20-30
30-40
Har.t. 0-5
5-10
10-20
20-30
30-40

Sand

20.8
19.7
17.2
18.8
18.3
36.2
37.5
36.2
33.7
36.2
45.8
43.1
45,9
43.2
39.2

Silt
" * 	
36.2
34.4
36.4
34.1
34.4
26.0
24.7
26.1
26.3
24.5
27.1
28,5
25.2
25.9
26.2

Clay

42.9
45.8
46.3
46.9
47.3
37.8
37.8
37.7
40.0
39.3
27.1
28.4
28,9
30.9
34.6

Texture
Clan

clay
clay
clay
clay
clay
clay loan
clay loam
clay loan
clay loam
clay loam
clay loan
clay loan
clay loan
clay loan
clay loaa

Bulk
Density
gin/cor*
1.37
1.31
1.20
1.21
1.22
1.32
1. 15
1.23
1.23
1.21
1.37
1.58
1.45
1.44
1.43
P
V

7.64
7.70
7.67
7.75
7,84
8.17
8.34
8.54
8.75
8.33
7.87
8.02
8.10
8.06
«. 30
H
0,01 M
CaClj

7.20
7.08
7.05
7.05
7.11
7.65
7.67
7.75
7.82
7.88
7.45
7.53
7.85
7.59
7.71

Carbonate
COj-C
X
2.59
2.72
2.76
1.94
2.09
1.81
1.90
2.01
2.54
4.22
1.33
1.55
1.50
1.79
5.50

Organic
Matter
*
3.16
1.91
1.39
1.18
1.18
2.53
2.44
1.94
1.40
1.13
3.36
2.25
1.85
1.67
1.16
Cation
Exchange
Capacity
B«J./100g
17.43
16.25
15.87
16.61
16.49
23.12
24.22
21.50
17.26
15.52
20.90
17.29
16.00
16.53
10.91

Soluble
Salt
Jlmhot/ca
720
620
770
710
660
273
252
200
179
183
399
315
273
252
252

-------
Organic Matter

     The organic matter content of all three soils in the upper 5  cm ranges
from 2.5 to 3.4 percent and drops to about 1.1 percent below 30 cm depth.
Prasad et al.  (1976) found that in alkaline and calcareous soils natural
chelating agents play an important role in the self-diffusion of zinc.  In
his study of 75 pasture soils of Spain, Macias (1973) found a significant
positive correlation (5 percent level) between organic matter and  total
zinc and between organic matter and IN NH.OAc extractable Cu.  He  found a
negative correlation, significant at the 5 percent level, between  organic
matter and 0.1 Ni HC1 extractable Cu.  Martens (1968) suggested that Cu
bonded to organic matter is the main source of plant available Cu.
John (1972) reported that extractable zinc in soil is increased with higher
organic matter and is one of the important soil properties in predicting the
zinc content of corn and oat plants.

Carbonate

     All three sewage farm soils have free CaCO- throughout the profile.
Angelo and Mereta soils have high carbonate content (4.22 and 5.50 percent
CO--C, respectively) at a soil depth between 30 to 40 cm.  The average
carbonate content of 120 sewage farm surface soils is 1.78 ± 0.68 percent
as CO~-C.  In their study of zinc adsorption by calcareous soils,  Udo et al.
(1970; found that the carbonate equivalent and organic matter content of
the soil influenced the Langmuir adsorption maxima of zinc by calcareous
soils.  Griffin and Shrimp (1976) reported that both cation exchange
capacity and carbonate precipitates were the principal mechanisms
affecting metal attraction by clay minerals in landfill leaching.  Ellis
(1974) stated that after prolonged wastewater applications most of the
heavy metals will be expected to precipitate as a hydroxide or a car-
bonate in the soil with a pH greater than 7.0.

Cation Exchange Capacity

     The cation exchange capacity of Mereta soil ranges from 20.90 meq/100 g
at the surface to 10.95 meq/100 g at the 30-40 cm depth.  Angelo soils
have the highest average cation exchange capacity (ranging from 24.22 to
15.52 meq/100 g) of the three soils.  The cation exchange capacity of
Rioconcho soils ranges from 17.43 to 16.49 meq/100 g.  The cation exchange
capacity of 120 surface soils at the sewage farm was 18.4 ± 4.1 meq/100 g.
The cation exchange capacity is significantly different at the 1 percent
level from that of 60 surface soils from the control area (20.2 ± 3.6
meq/100 g) as shown in Table 29.  Haghiri (1974) found the plant uptake of
cadmium could be influenced by several factors such as organic matter and
cation exchange capacity.  Korte et al. (1976) studied the influence of
soil chemical properties on trace element movement in soils of 7 major
orders.  They found significant correlations between cation exchange
capacity and adsorption of Ni and Zn by soils.  No significant correlation
between cation exchange capacity and adsorption of Cd and Cr was observed.
They concluded that the value of cation exchange capacity for predictive
purposes with natural soils is limited.  There was a highly significant
difference at the 1 percent level in cation exchange capacity of surface

                                     78

-------
TABLE 29.  STATISTICAL ANALYSIS OF SURFACE SOIL CHEMICAL CHARACTERISTICS BY  F TEST
                               Cation
            Organic           Exchange  Water
  Effect    matter  Carbonate Capacity Soluble  Total  Total  	IN HC1  extractable	
                                          P       P      N      Cd    Cr    Cu    Pb      Ni    Zn


Soil
Series  (A)   0.26    18.52C    9.32C    6.69°   2.54a   1.45  10.86C 5.63C 3.89  1.77   23.24C  2.38a

Wastewater
treatment (B)0.03      3.30a    9.20C  417.78C 118.23°   0.27  14.28C 4.46  0.15  1.89    0.46   1.54

Soil
Depth  (C)    11.83      7.32°    2.91b    1.06   18.58C  10.49°  3.62b 5.82° 2.46a  2.37a   1.82   1.78

A,  B
interaction  2.61a   40.23°    0.01     0.69   12.17°   3.04a  8.89° 4.15b 2.21   0.71   16.44°  1.10

A,  C
interaction  0.49      1.73     0.40     0.62    0.93    0.62   1.43  0.96  0.99   1.25    0.29   1.50

B,  C
interaction  2.57&     0.12     1.73     0.17    8.50°   2.63a  1,32  2.49a 0.75   1.06    0.89   0.86

A,  B,  C
interaction  0.55      0.46     0.84     0.28    0.79    0.62   1.44  0.50  0.42   0.56   0.97    0.73

Significant at 10  percent level

  significant at 5 percent level
Significant at 1 percent level
  18C  surface soil samples are used for calculation

-------
soils among three soil series.  The difference in cation exchange capacity
at varying soil depths is significant at the 5 percent level.

Soil Salinity

     The amount of soluble salt in all three soils is low, and no salinity
effect on plants is expected.  The conductivity of a saturated paste
extract was less than 1 mmho/cm.

Statistical Relationships Between Surface Soil Properties

     The effects of soil series, wastewater treatment, and soil depth on
the soil chemical characteristics were analyzed statistically and are
listed in Table 29.  Partial correlation coefficients among the 12 soil
chemical parameters are calculated and listed in Table 30.  These partial
correlation coefficients will be used to evaluate the best relationships
among all 12 soil characteristics.

     It was found that wastewater treatment and soil series have no effect
on the soil organic matter content; whereas soil depth has a significant
effect at the 5 percent level.  Partial correlation coefficients among
soil organic matter and total nitrogen, total phosphorus, and cation
exchange capacity are highly significant at the 0.1 percent level (Yp =
0.985, 0.585, and 0.569, respectively).

     There was a significant difference at the 10 percent level between
the carbonate content of soils in the sewage farm and that of the control
area.  It was found that soil series, soil depth, and interaction of soil
series and wastewater treatment have a highly significant (at the 1 percent
level) effect on the carbonate content of these surface soils (Table 29).
A highly significant negative partial correlation at the 1 percent level
between carbonate content and HCl extractable Ni, Cu, Cr (Yp = -0.504,
-0.233, and -0.337^ respectively), and between carbonate content and cation
exchange capacity (Yp = -Q.213) was found.

IN HCl Extractable Heavy Metals

     Lagerwerff (1971) showed that a chemical ind6x using IN HCl as an
extractant could be used to examine the availability of heavy metals to the
plants.  He found that increasing the soil pH from 5.9 to 7.2 resulted in
decreases in yield and heavy metal content of the radish plant.  Average
concentrations of 111 HCl extractable Cd, Cr, Cu, Ph, Ni and Zn are presented
in Table 30 for the San Angelo sewage farm and the control area soil.

     Data presented in Table 29 show that irrigation of land with sewage
wastewater can significantly affect the acid extractable Cd and Cr in the
surface soils.  No significant effect of wastewater irrigation on IN HCl
extractable Cu, Pb, Ni, and Zn of soils is observed.  The average IN HCl
extractable Cd and Cr concentration of 120 surface soils at the sewage farm
was 0.48 ± 0.22 and 0.49 ± 1.09 yg/g, respectively, and that of 60 surface
soils in the control area was 0.38 ± 0.07 and 0.21 ± 0.22 yg/g, respectively.
Application of sewage wastewater on land significantly increased the acid


                                     80

-------
              TABLE  30.   AVERAGE CONCENTRATIONS OF  IN  HC1 EXTRACTABLE Cd, Cr, Cu, Pb,  Ni,  and Zn
                   IN  THREE SOIL SERIES FROM THE  SAN ANGELO SEWAGE FARM AND THE CONTROL AREA
CO
Rioconcho v
Elements
Cd Mean
Standard Deviation
Maximum
Minimum
Cr Mean
Standard Deviation
Maximum
Minimum
Cu Mean
Standard Deviation
Maximum
Minimum
Pb Mean
Standard Deviation
Maximum
Minimum
Hi Mean
Standard Deviation
Maximum
Minimum
Zn Mean
Standard Deviation
Maximum
Minimum
Sewage*1
farm

0.38
0.07
0.50
0.28
0.13
0.21
0.81
0.00
0.55
0.07
0.78
0.47
4.76
1.44
8.67
3.09
2.04
0.27
2.89
1.79
0.73
0.63
2.89
0.37
Control
farm

0.38
0.06
0.45
0,17
0.11
0.05
0.14
0.00
0.50
0.06
0.52
0.25
4.68
0.76
5.69
2.28
1.71
0.20
1.80
0.87
0.41
0.07
0.47
0.21
.Angelo ,_
Sewage"
farm
• *••• *tm* Ml* » w ••••• 1 I Cf i
0.46
0.05
0.54
0.36
0.90
1.40
7.05
0.00
1.13
1.09
5.22
0.47
17.75
34.17
208.64
3.12
2.46
0.58
3.33
1.62
5.48
8.26
39.02
0.33
Control
farm
fa — -
0.41
0.04
0.48
0.36
0,09
0.11
0.26
0.00
0.52
0.12
1.02
0.47
5,38
1.07
8.99
4.71
2.01
0.42
3.12
1.66
0.48
0.39
1.97
0.31
Mereta
Sewage "*
farm

0.60
0.34
2.40
0.36
0.43
1.15
6.56
0.00
1.04
2.45
15.83
0.45
36.81
133.90
833.36
4.09
2.38
0.67
5.05
1.74
45.72
178.56
1096.60
0.35
Control15
farm

0.35
O.C8
0.46
0.21
0.42
0.26
0,65
0.00
1.48
0.74
2.51
0.46
7.03
2.76
16.67
4,15
3.00
0.72
4.19
1.37
1.64
1.06
4.C4
0.29
        from a total of 40 samples.

        from a total .of 20 samples.

-------
extractable Cd and Cr concentrations in these soils.  There is a highly
significant partial correlation at the 1 percent level between any one of
these acid extractable heavy metals (Cr, Cd, Cu, Pb, Ni and Zn) and other
heavy metals (Table 31).  The partial correlation coefficients (a total  of
15 Yp) between these six IN HC1 extractable heavy metals ranges from
0.427 to 0.970.  The IN HCl extractable Zn, Pb, and Cd are highly corre-
lated with total soil nitrogen.  There is significant partial correlation
(at 5 percent level) between acid extractable Cd and total soil phosphorus,
and between acid extractable Cu and total soil nitrogen.   Water soluble
phosphorus is significantly correlated at the 1 percent level with acid
extractable Cd, Cr,. Cu, Ni, Pb, and Zn.

     A highly significant partial correlation was found between organic
matter content and IN HCl extractable Zn and Cd concentrations (Yp - 0.206
and 0.290, respectively).  Some heavy metals are expected to be retained
in the soil by organic matter.  Many organic materials are capable
chelating the heavy metals and increase their concentration in soil
solution.  This will result in increased uptake by plants and increased
leaching through the soil profile into the groundwater (Ellis 197'

     There was a highly significant positive partial correlation ^. the
1 percent level between cation exchange capacity and HCl-extractable Ni
(Yp = 0.205), total N(Yp = 0.546), total P(Yp = 0.440), and water
soluble phosphorus (Yp = 0.260 (Table 31).
NUTRIENT LOADING OF SOIL PROFILES

     Soil cores were taken from the sewage farm and from a control area
adjacent to the farm.  Sampling areas were chosen that represented the
major s-oil series (Angelo, Mereta and Rioconcho) .  Ten cores were taken
on the farm where sewage effluent has been applied for the past 18 years.
Five cores were taken from the control area which had been grazed but had
not been cultivated or received sewage effluent.  Soils were taken to a
depth of 200 cm, except where a gravel or hard  caliche layer existed  in
the soil profile.  The location of cores from the sewage farm and from
the control area is shown in Figure 10.  The cores were sectioned and
each section analyzed for total nitrogen, NH,-N, NOl-N, NO~-N, total P,
water-soluble P and total heavy metals (Cd, Cr, Cu, Pb, Ni, and Zn).  A
comparison of the two sets of profile samples were used to assess the
nutrient loading which occurred as a result of sewage effluent irrigation.
The total nutrient contents were calculated using the concentration of
total nutrients and soil bulk density at each depth.  Because only two
soil profiles, one in the sewage farm and another one in the control
area, from each soil series have been used for the determination of bulk
density and that value of bulk density has been used for all soil profiles
of the same soil series, the total soil nutrient contents are only an
estimate.  The bulk densities of three soil series from the San Angelo
sewage farm and the control area are shown in Table 32.
                                     82

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           TABLE  31.   PARTIAL CORRELATION COEFFICIENTS AND SIGNIFICANT PROBABILITY LEVEL OF CHEMICAL
              CHARACTERISTICS  OF SURFACE SOILS .(0 - 20  cm)  IN THE SEWAGE FARM AND THE CONTROL AREA *
00

HCl-Zn
HC1-S1
HCl-Pb
HCl-Cu
HCl-Cr
HCl-Cd
Total-N
Total-P
H,0-P
Cation
Exchange
Capacity
Carbonate
Organic
Matter
0.20625
0.009
0.07681
O.J38
0.16246
0.042
0.15778
0.048
0.12401
0.121
0.28989
0.000
0.9S547
0.000
0.53451
0.000
0.24841
0.001
0.56912
0.000
-0.02237
0.780
Carbonate
—0.09328
0.245
-0.50388
0.000
-0.13023
0.104
-0.23309
0.003
-0.33724
0.000
0.03603
0.654
-0.02288
0.776
0.15205
0.057
-0.11978
0.135
—0.21312
0.007

Cation
Exchange
Capacity
-0.04209
0.600
0.20542
0.009
-0.03111
0.69S
0.05980
0.456
0.16261
0.041
-0.07230
0.368
0.54626
0.000
0.44030
0.000
0.26001
0.001


H20-P
0.38285
0.000
0.27360
0.000
0.39035
0.000
0.40938
0.000
0.33240
0.000
0.30061
0.000
0.27291
0.000
0.25942
0.001



Total-P Total-N HCl-Cd HCl-Cr HCl-Cu HCl-Ri HCl-Pb
0.06J.23 0.26073 0.90283 0.54849 0.90141 0.96953 0.46173
0.42.'. 0.001 0.000 0.000 0.000 0.000 0.000
-0.10004 0.08934 0.42692 0.63813 0.6123t- 0.49921
0.213 0.265 0.000 0.000 0.000 0.000
0.05179 0.20327 0.85783 0.69533 0.94989
0.51S 0.010 0.000 0.000 0.000
0.04108 0.19166 0.76194 0.78387
0.60S 0.016 0.000 0.000
0.07566 0.11919 0.44750
0.346 0.137 0.000
0.19S27 0.33983
0.012 0.000
0.60671
0.000




         'From 180 soil samples

-------
Figure 10.  Sampling locations for soil cores taken from the
 sewage farm and for soil cores taken from the control area
outside the sewage farm (A = Angelo,  M = Mereta, R = Rioconcho)
                              84

-------
TABLE 32.   DISTRIBUTION OF BULK DENSITY WITH DEPTH IN THREE SOIL
           SERIES FROM THE SAN ANGELO SEWAGE FARM AND THE CONTROL
           AREA

Location





Sewage
farm






Control
area




Depth


0-5
5-10
10-15
15-20
20-30
30-40
40-50
50-100
0-5
5-10
10-15
15-20
20-30
30-40
40-50
50-100

Rioconcho


1.37
1.31
1.23
1.18
1.21
1.22
1.31
1.31
1.18
1.26
1.20
1.26
1.25
1.28
1.38
1.21
Bulk density3
Angelo
3

1.36
1.58
1.39
1.50
1.44
1.43
1.42
1.38
1.25
1.07
1.10
1.01
1.20
1.14
1.13
1.23

Mereta


1.32
1.15
1.37
1.08
1.23
1.21
1.22
—
1.36
1.34
1.42
1.43
1.49
1.50
1.62

  from a single measurement
                                85

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Total Nitrogen

     The total nitrogen (N) concentration ranges from 0.06 to 0.5 percent  in
the top 15 cm of most cultivated soils.  Most of the total N is in  the
organic form.  Ammonification and nitrification of organic N may signifi-
cantly increase the NH.-N, N02~N, and NOo-N concentrations in the soil
solution.  Irrigation with wastewater on land may become a potential
environmental hazard if the crop removal of applied N is small, and the
applied N leaches , into the surface or ground waters.

     A study by Johnson et al. (1975) indicated that growing soybeans
resulted in a net removal of N from the soil, especially when the soil
contained residual inorganic N from a preceding crop.  Stanford (1973)
reported that with good management the grain and stover of corn could
recover 50 to 70 percent of the applied N.  He showed that if optimum rates
of N were applied on corn, all residual N would be immobilized.  Immobiliza-
tion of applied N by microorganisms and plants will reduce the potential of
leaching of N and removals of N on cropland in 8 Great Plains and Western
States in the United States.  They found that the weighted average  of the
ratio N fertilizer applied to nitrogen removed was 0.82 for this region.
This ratio for other states in Central, East, and Southeast regions of  the
United States ranged from 0.59 to 4.90.

     The distribution of total N concentration with depth in the sewage
farm soils compared to the control area is shown in Figure 11.  The
maximum total N concentration was found in the top 0-5 cm layer and
generally decreased with increased soil depth in all soils.  There  is a
significant difference (at the 1 percent level) in total N concentrations
among the three soil series and among soils at varying depths (Table 33).
No significant difference is found in total N concentration between the
sewage farm soils and the control area soils or among the soil cores
sampled.  Irrigation with sewage wastewater did not significantly change
the total N concentration of Rioconcho, Angelo, and Mereta soils.   In
Rioconcho soils removal of applied N is greater than the N applied  to the
soil.  In Angelo and Mereta soils there is an accumulation of total N in
the upper 0-5 cm of surface soil.  The total N content at varying soil
depths in the soil profile in Rioconcho, Angelo, and Mereta soils is listed
in Tables 34 , 35 , and 36 , respectively.  The average total N content-of
the upper 20 cm of soil increased 38.9 percent in Angelo soils, and 26.6
percent in Mereta soils after 18 years of wastewater irrigation and crop
production (Table 37).  This increase was due primarily to an accumulation
in the top 0-5 cm.  There was a decrease of 19.6 percent of total N
content in the top 20 cm soil column in Rioconcho soils.  Total N content
was less for the Rioconcho soils in the sewage farm at all depths.  The
overall average total N content of the top 20 cm of soil in these
three soil series increased 13.4 percent.

Exchangeable Inorganic Nitrogen

     Ammonium (NH,)  and nitrate (NO,) are the most important forms  of N
utilized for plant growth.  Generally, only a small proportion of total
soil N is in the inorganic forms, if the nonexchangeable NH. .fixed  by soil

                                     86

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                  RIOCONCHO
             ANGELO
                              MERETA
     DEPTH
        cm
00
                      1250
2500
  TOTAL-N,^g/g
0       12.50    2500
I £50
2500
                                     SEWAGE FARM SOIL
                                     CONTROL  SOIL
         Figure 11.  The effect of sewage effluent irrigation on total nitrogen content of the top
                   200 cm of soil from the sewage farm compared to the control area.

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  TABLE 33.   STATISTICAL RELATIONSHIPS BETWEEN SOIL NUTRIENTS  AS RELATED TO SOIL SERIES,

                       TREATMENT,  CORE AND DEPTH AS DETERMINED BY ? TEST
Effect Total-N NO~-N NOj-N
Soil Series 7.08° 7.86° 1.66
Treatment 0.09 12.05° 0.46
c
00 Core 0.82 5.80 1.28
00
Depth 17.42° 1.04 0.86
KH^-N Total-:?
2.19 5.26':
0.10 127.37°
b ,.
2.14 3.06'-

0.82 22.60°
H20-P Cd Cr Cu Ni Pb Zn
2.25 12.99° 1.19 2.28 126.55° 28.58° 5.31°
528.16° 2.10 5.99b 3.29a 57.61° 11.46° 2.19
c c a c c c a
7.30 2.76 1.71 5.29 3.96 2.49 1.73

13.48° 19.44 1.67* 0.59 24.33° 0.80 1.72*
"significant at 107. level

b
 Significant at 57, level


Significant at 1% level


dFrom 408 Soil Samples

-------
     TABLE 34.   DISTRIBUTION OF NUTRIENTS WITH DEPTH IN RIOCONCHO SOIL CORES TAKEN FROM THE
                SEWAGE FARM AND FROM AN ADJACENT CONTROL AREA
oo
Depth

0-5
5-10
10-15
15-20
20-30
30-40
40-50
50-100

0-5
5-10
10-15
15-20
20-30
30-40
40-50
50-100
Total-P

763.77
569.19
486.46
433.65
S61.52
824.72
839.71
3668.00

401.79
412.02
376.20
377.37
696.25
661.76
673.44
3043.15
V*

15.99
14.47
13.06
11.76
22.13
20.38
20.18
63.79

2.25
1.48
0.86
0.72
1.47
1.33
1.25
4.29
Total-N

1298.76
831.85
698.02
600.62
1090.21
883.28
877.70
3530.45

1227.20
1255.59
915.60
869.40
1585.00
1288.96
1188.18
5541.80
NOj-N

7.69
6.39
7.06
5.95
16.28
13.84
11.59
50.56

4.55
5.24
3.27
6.91
10.80
4.50
3.56
17.54
NO~-N

0.07
0.05
0.06
0.04
0.09
0.09
0.09
0.48

0.13
0.24
0.11
0.14
0.22
0.19
0.19
0.87
NH+-N
4
•" 	 Ks/1
SEWAGE
0.54
0.85
0.86
1.00
0.60
0.00
0.52
2.62
CONTR01
0.41
0.00
0.00
1.07
1.62
3.20
0.00
0.00
Cd
la ------
FARM
0.73
0.71
0.65
0.63
1.34
1.41
1.53
8.18
L AREA
0.80
0.84
0.82
0.86
1.73
1.71
1.84
8.77
Cr.

15.35
13.78
12.86
12.40
25.05
24.96
26.10
131.00

9.92
11.17
10.12
10.59
21.31
20.31
20.17
97.22
Cu

8.12
7.75
7.76
8.04
16.15
18.62
21.47
219.29

6,33
7.06
6.54
7.16
13.81
13.35
13.09
66.24
Nl

9.35
8.86
8.25
8.02
16.69
17.05
19.00
98.44

8.64
9.75
9.33
9.78
19.63
19.96
20.87
98.61
Pb

15.20
13.87
12.34
11.12
21.93
22.70
25.02
122.94

12.70
13.41
12.83
13.60
27.55
25.12
25.84
122.45
Zn

30.14
26.57
24.65
23.64
46.25
46.61
50.50
303.65

27.09
29.85
26.37
27.09
48.85
53 . 64
54.42
250. S4

-------
TABLE  35.   DISTRIBUTION OF NUTRIENTS WITH  DEPTH IN ANGELO SOIL CORES  TAKEN FROM  THE
            SEWAGE  FARM AND FROM AN ADJACENT CONTROL AREA
 Depth
Total-P
H20-P
Total-N
N03-H
NOj-N
                                    Cd
                                            Cr
                                                                       Cu
                                                                                          Pb
                                                                                                   Zn
                                                           Kg/ha
                                                          SEWAGE FARM



VD
o



0-5
5-10
10-15
•15-20
20-30
30-40
40-50
50-100
1024.76
842.93
548.35
513.75
915.84
898.04
866.20
3346.50
11.27
12.91
12.22
13.62
24.71
22.95
18.80
80.17
1723.12
1231.61
987.59
967.50
1696.32
1468.61
1324.86
4319.40
14.17
7.77
5.11
5.07
9.70
7.65
7.51
26.97
0.10
0.08
0.07
0.08
0.18
0.18
0.15
0.71
1.29
0.23
0.00
1.05
1.58
0.71
2.1.3
6.21
0.55
0.69
0.63
0.67
1.42
1.63
1.86
11.79
16.23
15.71
14.57
14.40
27.87
24.78
25.27
102.60
8.16
8.80
7.51
7.88
14.84
14.85
14.73
66.17
9.73
11.82
10.73
11.56
24.04
26.21
26.83
138.82
25.34
25.58
19.78
18.89
26.79
26.52
28.44
142.55
37.58
39.69
32.40
33.36
58.63
55.02
52.44
226.66
                                                          CONTROL AREA
0-5
5-10
10-15
15-20
20-30
30-40
40-50
50-100
247.50
219.88
206.80
181.29
423. 60
384.18
360.47
1906,50
0.50
0.32
0.26
0.33
0.74
0.49
0.35
0.73
1093.75
859.21
799.70
783.25
1452.00
1295.04
1111.92
4040.55
2.66
2.16
2.29
2.85
6.84
7.22
7.07
52.02
0.21
0.23
0.14
0.13
0.38
0.26
0.26
1.46
4.37
3.63
2.42
3.28
8.76
6.15
7.57
27.67
0.74
0.62
0.65
0.64
1.57
1.53
1.71
11.80
9.43
8.20
9.27
9.27
25.06
24.48
22.37
88.31
7.78
6.72
6.12
5.67
13.46
12.22
11.96
62.11
10.59
9.25
9.71
9.46
22.99
22.68
23.53
134.43
11.25
9.15
9.42
8.96
22.04
21.32
21.78
132.77
29.28
23.74
23.75
21.46
51.19
49.12
48.90
218.57

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TABLE 36.  DISTRIBUTION OF NUTRIENTS WITH DEPTH IN MERETA SOIL CORES TAKEN FROM THE
           SEWAGE FARM AND FROM AN ADJACENT CONTROL AREA
Depth


0-5
5-10
10-15
15-20
20-30
30-40
40-50

0-5
5-10
10-15
15-20
20-30
30-40
40-50
Total-P


1180.74
733.70
669.93
438.48
836.40
655.82
642.94

212.84
214.40
250.63
255.97
505.11
535.50
596.16
H20-P


14.66
10.49
12.02
9.04
15.32
14.37
14.56

0.33
0.14
0.19
0.1R
0.4b
0.42
0.37
Total-N


2333.10
980.95
940.50
695.52
2277.96
975.26
810.08

1024.08
982.22
959.92
945.23
1737.34
1638.00
1814.40
NO^-N


20.98
15.08
35.66
11.94
23.37
23.58
24.32

0.88
0.57
1.10
1.24
2.17
2.07
3.28
N02-N


1.37
0.08
8.29
0.08
0.13
0.13
0,16

0.05
0.07
0.22
0.08
0.06
0.14
0.18
NflJ-N

SEWAGE I
26.26
0.46
19.52
0.54
0.00
0.60
1.22
CONTROL
0.40
0.00
0.63
0.00
1.19
1.05
2.43
Cd

'ARM
1.00
0.73
0.80
0.72
2.17
2.27
2.54
AREA.
0.53
0.51
0.57
0.65
1.63
1.89
2.13
Cr


42.27
13.98
13.45
9.48
23.03
16.26
15.15

10.15
10.31
11.16
11.57
24.68
26.38
28.80
Cu


21.33
9.49
9.16
6.81
14.77
10.75
9.79

6.70
7.14
7.40
7.60
14.91
15.51
17.82
Ni


9.39
8.17
10.40
8.62
22.69
22.66
23.42

9.22
9.82
10.74
11.93
25.01
26.34
30.52
Pb


268.75
118.62
135.63
115.50
225.09
438.38
230.58

9.87
8.64
8.97
9.76
21.96
23.20
26.70
Zn


204.07
57.28
41.40
27.01
67.28
40.02
34.41

28.85
26.51
27.48
27.34
53.95
55 77
** ^ " i /
62.03

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         Table 37.   TOTAL NUTRIENT CONTENTS OF THE TOP 20 CM SOIL SOLUMS FROM THE SAN ANGELO SEWAGE
                    FARM AND THEIR RELATIVE CHANGE AFTER 18 YEARS OF IRRIGATION WITH SEWAGE EFFLUENT
                    COMPARED TO AN ADJACENT CONTROL AREA
to
RIOCONCHO
Nutrient
Total-P
H20-P
Total-N
KO~-N
SO~-N
K-"
Total-Cd
lotal-Cr
Total-Cu
Total-Ni
Total-Pb
Total-Zn
Sewage
farm

2253.09
55.30
3429.26
27.11
0.24
3.26
2.73
54.41
31.69
34.51
52.56
105.02
Control
area
_« !f«.1
221.9
-52.7
-81.2
-4.5
65.7
22.9
12.3
130.9
45.6
Sewage
farm

3022.85
46.23
4950.08
83.68
9.85
46.79
3.28
79.20
46.81
•36.61
638.51
329.77
MERETA
Control
area
L'o/fia __.
933.84
0.86
3911.45
3.81
0.44
1.05
2.29
43.22
28.85
41.73
37.24
110.20
Difference

2089.01
45.37
1038.63
79.87
9.41
45.74
0.99
35.98
17.96
-5.12
601.27
219'.57
223.7
5275.6
26.6
2096.3
2138.6
4356.2
43.2
83.2
62.3
-12.3
1614.6
199.2
ALL SOILS
Difference
Kg/ha
4849.04
143.94
1574.01
109.14
8.61
36.38
0.26
72.72
28.58
-3.32
652.07
258.97
144.5
1886.5
13.4
323.1
470.5
223.9
3.1
59.7
34.7
-2.8
507.1
81.2

-------
is not included.  The  amount  o£ nitrite (N0~)  is generally very small
compared with the amount  of NH4 or N0~   Application of  high rates  of M*
may result in N02 accumulation in alkaline soils (Bremner  1965b).

     Wb,en sewage wastewater is applied to a predominantly  aerobic soil,
fhe NH4 in the  sewage  effluent will be converted to  N0~  by the  nitrification
process.  The applied  N can be removed by plant  uptake;  denitrification,
leaching, and ammonia  (NH ) volatilization.  Denitrification is the most
important process whereby the applied N in excess of plant uptake can be
removed from the soil  (Lance  1972).  Long periods of flooding with  sewage
wastewater can  stimulate  the  denitrification process in  the  soil.   Stefanson
(1973) showed that  crops  may  increase N removal  by stimulating  denitri-
fication in the root zone due to exudation of  organic carbon and the
creation of low oxygen levels.   Nitrate,  which was neither denitrified nor
taken up by plants, will  move down to the ground water.  Thomas et  al.
(1974) found that a well  operated overland flow  system could achieve 90
percent N removal in the  summer, but N removal may drop  substantially in
the winter.                         1

     The distribution  of  2N KCl exchangeable NH4-N,  NO~-N, and  N02-N with
soil depth in the sewage  farm and the control  area soil  are  shown in
Figures  12, 13, and 14, respectively.  In Angelo soils the NH.-N concen-
tration sampled from the  sewage farm is less than that from  the control
area.  In Rioconcho and Mereta soils the difference  in NH.-N concentration
between the sewage  farm soils  and the control  area soils varies with soil
depth.  There is an accumulation of NH.-N in the upper 15  cm soil in Mereta
soils of the sewage farm. There is no significant difference in NH4~N
concentration among three soil series and with soil  depth (Table 33).
Irrigation with sewage wastewater did not significantly  change  the  NHt-N
concentration of Rioconcho, Mereta, and Angelo,soils.  There is a signifi-
cant difference (at the 5 percent level)  in NH.-N concentration among soil
cores sampled.  The total exchangeable NH4~N content at  varying soil depth
in the soil profile of Rioconcho, Angelo, and  Mereta soils is shown in
Tables 34, 35,  and  36, respectively.   Irrigation with wastewater increased
by  120 and  4356 percent the total NH,-N content  in the soil  profile of
Rioconcho and Mereta soils (Table 37)+-  However, in  Angelo soils the total
NH.-N content on the soil profile decreased 81 percent.  The overall
average NH/-N content  of  the  top 20 cm soil column of these  three soil
series increased 224 percent.  Reneau  (1977)  studied  the  changes in
inorganic nitrogen  compounds  from septic tank+effluent in  a  Virginia coastal
plain soil.  He found  that the decrease in NH4~N was due to  the processes
of adsorption and nitrification.

     In both Rioconcho and Angelo soils the N02  concentration of sewage
farm soils is less  than that  of the control area soils (Figure  14) .   The
N0~ concentration of Mereta sewage farm soils  is greater than that  of the
control area.   There are  large amounts of N02  in the 0-15  cm layer  in the
Mereta soils.   There was  no statistically significant difference in N02
concentration among_the three soil series, soil  cores, or  soil  depths.
No difference in NO, concentration between the sewage farm soils and the
control area soils  is  found  (Table 33}.  The total exchangeable NO  -N
content at varying  soil depth in the soil profiles in Rioconcho, Angelo

                                      93

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                    RIOCONCHO
                       ANGELO
MERETA
       DEPTH
         cm
V£>
JS-
468  10    02468   10
i	i	.	.
                                                                  024   6   8   10

                                                                              '/ 39.75 1
                                    0SEWAGE FARM SOIL
                                    IB CONTROL SOIL
             Figure 12.  The effect of sewage effluent irrigation on exchangeable ammonium
         nitrogen content of the top 200 cm of soil  from the sewage farm compared to the control area.

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                   RIOCONCHO
      ANGELO
MERETA
      DEPTH
         cm
VO
Ui
                       10   15  20 25
   N03 -
05    10   15  20  25
                                   QSEWAGE FARM  SOIL
                                   • CONTROL  SOIL
           Figure 13.  The effect of sewage effluent irrigation on exchangeable soil nitrate nitrogen
            content of the top 200 cm of soil from the sewage farm compared  to the control area.

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             RIOCONCHO
            AN6ELO
                               MERETA
DEPTH
  cm
                 250
500
    N02-N,ng/g
0       250       500
250
500
                            QSEWAGE  FARM  SOIL

                            •[CONTROL  SOIL
  Figure 14.  The effect of sewage effluent irrigation on exchangeable nitrite nitrogen

     content of the top 200 cm of soil from the sewage farm compared to the control area.

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and Meireta soils is shown  in  Tables  34,  35,  and 36,  respectively.
Irrigation with wastewater decreases the total N0~ content  in  the  soil
profile of Rioconcho and Angelo  soils 63 and 53 percent,  respectively
(Table 37).  In Mereta  soils,  an increase of 2139  percent in total N0~
content is found after  wastewater irrigation.   Irrigation with wastewater
can change the NH^-N or NO^-N content in a soil.   However,  the change may
be positive or negative.   There  is an increase of  471  percent  of NO~-N in
the upper 20 cm soil column of all soils in the sewage farm after  18 years
of wastewater irrigation.

     The N03 concentration of Mereta soils at  the  sewage  farm  is much
greater than that  of the control area soils.  There  is an accumulation of
N0.j in the upper 50 cm  soil in the Mereta soils after  sewage irrigation.
In Rioconcho and Angelo soils the difference in NO~-N  concentration between
the sewage farm soils and  the control area soils varies with soil  depth.
Generally, the NO,, concentration of  sewage farm soils  decreases with in-
creased soil_depth.  There is a  significant difference .(at.the 1 percent
level) in N03 concentration among three  soil series  and among  soil cores.
No significant difference  in  NO«-N concentration is  found with varying soil
depth.  Irrigation with wastewater significantly (at the  1  percent level)
changed the NO- concentration of Rioconcho,  Angelo,  and Mereta soils (Table
33).  Data presented in Tables 34, 35, and 36  show the total NO~--N content
at varying soil depths  in  the soil profile of  Rioconcho,  Angelo,_and Mereta
soils, respectively.  In Mereta  soils the difference in total  NO,,  content
of the upper 20 cm of soil between the sewage  farm and the  control area
soils is  79.87 Kg/ha, an increase of 2096 percent  based on  the control area
soils (Table 37) .   In Rioconcho  soil the difference  in total NO,, content
in the upper 20 cm soil solum between the sewage farm  and the  control area
is 7.12 Kg/ha, an  increase of 36 percent.  In  Angelo soils  an  increase of
222 percent (22.2  Kg/ha) of total NO- content  in the upper  20  cm soil
solum is found.  Frere  (1976) reported that soils  cultivated for 100 years
can have drainage  waters with 5  to 10 ppm NO^-N.   Kreitler  (1975)  found
high NO, concentration  in  the ground water in  central  West  Texas.  His
study snowed that  the main source of NO, in this region was the mineral-
ization of organic soil N. Reneau (1977) observed N0~ accumulation in the
plinthic horizon beginning at a  distance of 1.27 m from the drainfield
when septic tank effluent  is  applied.

Total Phosphorus

     Total phosphate  (P) concentration in soils ranges from 100 to 1300 ppm.
Because of the low soil solution P (ranges from 0.01 to 1 ppm  P) and high
degree of fixation, soil P tends not to  leach i^_soils.=  The applied P
can be precipitated in  soils  as  Fe   , Al   ,  Ca   , C03> and  OH   phosphate
compounds.  In calcareous  soils  the  calcium phosphate  precipitate  may
be the main mechanism for  P removal  from wastewater  (Lindsay and Moreno
1960).

     Kao and Blanchar  (1973)  found that  soil fertilized with P for 82 years
had nearly double  the total P content, but the ability of the  soil to
adsorb additional  P was the same. No fertilizer P was found below 137  cm
in the soil profile.  Beek et al. (1977) studied distribution  of soil P


                                      97

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in soil from a sewage farm in the Netherlands treated with domestic and
industrial wastewaters.  They showed that applied P accumulated in the top
50 cm layer of the soil.

     The distribution of total P concentration with depth in the sewage
farm soils and in the control area soils is shown in Figure 15.  It is
clear that the maximum total P concentration is found in the top 0-5 cm
layer and decreases with increased soil depth in all three soil series in
the sewage farm.  No significant difference in total soil P with soil depth
was found in the soil profiles of the control area.  A comparison of the
distribution of total soil P concentration with depth between the sewage
farm and the control area indicates that applied P from sewage wastewaters
had accumulated in the upper 150 cm in both the Rioconcho and Angelo soils
and the upper 100 cm in Mereta soil.  The application of sewage wastewater
on land significantly increased (at the 1 percent level) the total P
concentration in the soil profile of these three soil series (Table 33).
There was a significant increase (at the 1 percent level) in total soil
P concentration among three soil series, soil depth, and soil cores sampled.

     Data in Tables 34, 35,  and 36  show the total P content at varying
soil depths in the soil profile in Rioconcho, Angelo, and Mereta soils,
respectively.  The average total soil P content in the whole soil profile
of Rioconcho, Angelo, and Mereta soils is listed in Table 37.   An increase
of 44 to 243 percent of total soil P in the soil profile was found in
these three soils after 18 years of sewage wastewater treatment.
Water Soluble Phosphorus

     Water soluble P is a useful index of soil P availability for plant
growth.  Adsorption of P and growth of plants increase as water soluble
P increases in soils low in P.  Many studies have shown significant corre-
lation between soluble soil water P and P uptake by plants (Olsen and Dean
1965).

     The distribution of water soluble P content with depth in the sewage
farm soils and in the control area soils is shown in Figure 16.  The
Rioconcho and Mereta soils inside the sewage farm have the highest water
soluble P concentration in the top 0-5 cm layer.  The P concentration
then decreases with increasing soil depth.  The highest water soluble P
concentration is found in the 15-20 cm layer in Angelo soil, located
inside the sewage farm.  The water soluble P concentration is about the
same in the whole soil profile of the Angelo and Mereta soils in the
control area.  Rioconcho soils from the control area have higher water
soluble P concentration in the surface and decreases with increased soil
depth.

     Comparison of the distribution of water soluble P concentration with
depth for the soils located in the sewage farm and control area indicates
that there is an active pool of water soluble P in the soil profile inside
the sewage farm.   Irrigation with sewage wastewater significantly increased
(at the 1 percent level) the water soluble P concentration in Rioconcho,


                                     98

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                    RIOCONCHO
      ANGELO
MERETA
       DEPTH
         cm
VO
                                15Op
TOTAL- P,  Mg/g

Q       750      I50C
                                       SEWAGE  FARM SOIL

                                       CONTROL SOIL
            Figure 15.  The effect of sewage effluent irrigation on total soil phosphorus

          content of the top 200 cm of soil from the sewage farm compared to control area.

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                    RIOCONCHO
                               AN6ELO
MERETA
       DEPTH
         cm
o
o
                       WATER SOLUBLE P,  >ug/g
0   5   1.0   1.5  2.0  35    05   10  15  2p  2.5    0  $   1.0  15  2.0  %5
                                       SEWAGE  FARM SOIL
                                       CONTROL SOIL
            Figure 16.  The effect of sewage effluent irrigation on water-soluble soil phosphorus
            content of 200 cm of soil taken from the sewage farm compared to the control area.

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Angelo, and Mereta soils  (Table 33).   There is a significant  difference
(at the 1 percent level)  in water soluble soil P concentration  among soil
depth and soil cores sampled.

     The water soluble P  content  at varying soil depths  in  Rioconcho,
Angelo, and Mereta soils  is shown in  Tables 34,  35,  and  36, respectively.
After 18 years of wastewater  irrigation,  the  average total  amount of
water soluble P in the soil profile increased 936  percent in  the Rioconcho
soil, 3399 percent in the Angelo  soil and 5276  percent in the Mereta" soil
The water soluble P increased 1887 percent in the top 20 cm of  all soils
in the sewage farm after  18 years of  wastewater irrigation.   The water
soluble P is greater than 15 yg/g P in the upper 20  cm layer  of Rioconcho,
Angelo, and Mereta soils  (Figure  16).   The percentage of water soluble P
based on the total P content was  also increased  in all three  soils in the
sewage farm.

     The distribution pattern with depth  and  the high concentrations of
water soluble P in the soil profile indicate  that the upper 40  cm layer
of Angelo soil may have reached its maximum adsorption potential with
regard to applied P in wastewater.  The upper 20 cm  layer has been
saturated with respect to water soluble P,  in Rioconcho  and Mereta soils.
It is clear that the upper 200 cm soil column has been affected to some
degree by the applied P in wastewater.  When  the P fixation capacity of
the soil is not sufficient to handle  the  desired volume  of  wastewater,
the P-containing water will be filtered through the  soil profile and
reach the groundwater.

Total Heavy Metals

     Heavy metals can influence environmental quality by their  potential
hazard and toxicity to man and other  living organisms.   Heavy metals in
soils and plants are the  major link in the heavy metal cycle  in the food
chain.  One of the unknown factors of land application of wastewater is
the contamination and accumulation of heavy metals in the soil, especially
in regard to long-term effects.   Lisk (1972)  warned  that using  sewage
wastes for plant fertilizer might be  a dangerous practice.  He  pointed out
that some edible plants may cpncentrate toxic heavy  metals  present in
wastes to a level which would not harm plants but which  could be toxic to
animals.  Reefer and Estepp  (1971) found  most of the labeled  Zn-65 applied
to soils became acid-soluble  and/or fixed on  the clays.  Fuller et al.
(1976) reported that the  municipal landfill leachate was relatively low
in heavy metals and that  those present did not migrate through  the soils
in significant quantities.  They  found that all soils examined  did not
release heavy metals at the same  rate. A study at the sewage farm in
Melbourne, Australia showed that  large amounts of metals have accumulated
during 70 years of application of raw and settled sewage (Bouwer and
Chaney 1976).  It is clear that soil-plant systems play  an  important
role in the renovation of wastewater.
                                     101

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Cadmium

     Cadmium  (Cd) levels in non-polluted  soil are usually below 1  ppm
(Haan and Zwerman 1976).  Buchaver  (1973)  found  1,700  ppm Cd  in topsoil
sampled near  a zinc smelter.  The Cd concentration  in  phosphate fertilizers
ranges from 1-2 ppm for tertiary calcium  phosphate  to  50-170  ppm for
superphosphate.  Miller et al.  (1976)  investigated  the relationship between
uptake of Cd  by soybean and soil available P and found that Cd  accumulation
increased with increasing available soil  P.  Williams  and David (1976)
reported that at least 80 percent of the  Cd impurities in P fertilizers
applied could be accounted for  in the  cultivated layers of soil.   The
sources of soil Cd are varied but may  come from  the application of wastes
and P fertilizer, fallout, precipitation,  decomposition of plant residues
and microorganisms, and weathering  of  soil parent materials.

     The distribution of total  Cd concentration  with soil depth in the
sewage farm soils and the control area soils is  shown  in Figure 17.  There
is a highly significant difference  in  total soil Cd concentration among
the three soil series, among soil cores sampled, and at different  soil  depths
 (Table 33).   However, comparison of the total Cd concentration  of  soils
in the sewage farm and the control  area indicates no significant change
in total soil Cd concentration  after 18 years of wastewater irrigation.  The
average total amount of Cd content  in  the upper  20  cm  soil solum of the
sewage farm changes -19.3 percent in Rioconcho soils,  -4.5 percent in
Angelo soils, and +43.2 percent in  Mereta soils  (Table 37).   This amounts
to a 3.1 percent increase of all top 20 cm soils of the sewage  farm after
 18 years of wastewater treatment.

Chromium

     Chromium (Cr) has been considered nonessential for plant growth;
whereas it is essential to animals  and man.  Chromium  plays an  important
role in carbohydrate metabolism and prevention of diabetes.   Chromium
reaches the soil through sanitary landfills, urban  air,  snow  melting salts,
industrial and sewage wastes, and weathering of  soil parent material.   The
Cr content of most soils is usually limited to trace amounts.   In  soils
treated with  sewage sludges the Cr  concentration can reach up to 50,000
ppm (Walsh et al. 1976).  Dowdy and Larson (1975) found  that  Cr from sewage
sludges is less available to barley plants grown in alkaline  soils compared
to those grown in acid soils.   Lindsay (1973) reported that Cr  is  generally
oxidized from Cr(II) or reduced from Cr(VI) to Cr(III) and precipitated as
an insoluble  hydroxide.  Bartlett and  Kimble (1976)  showed that the presence
of soil organic matter brought  about spontaneous reduction of Cr(VI)  to Cr
(III) even at pH's above neutrality.

     The distribution of total  Cr with soil depth in the sewage farm and
the control area soils is shown in  Figure  18.  In Mereta soils  sampled
at the sewage farm,  a small amount  of  Cr  (32.12  Kg/ha) was accumulated  in
the top 0-5 cm (Table 36).   There is a significant  difference (at  the
5  percent level)  in total Cr concentration between  the sewage farm soils
and the control area soils.   A significant difference  (at the 10 percent
                                     102

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             RIOCONCHO
                      ANGELO
             MERETA
DEPTH
  cm
        o.oo
1.25
       TOTAL-Cd,
250    0.00      1,25
2,50  0.00
2.50
                             O SEWAGE FARM SOIL
                             • CONTROL SOIL
          Figure 17.  The effect of sewage effluent irrigation on total soil cadmium
       content of 200 cm of soil taken from the sewage farm compared to the control area.

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             RIOCONCHO
      ANGELO
   MERETA
DEPTH
  cm
                    15  20  25
TOTAL-Cr, flg/g

0   5   10   15  2,0  2,5
5   10  15  2,0 25
                            QSEWAGE FARM  SOIL

                            •B CONTROL SOIL
        Figure 18.  The effect of sewage effluent irrigation on total soil chromium

      content of 200 cm of soil taken from the sewage farm compared to the control area.

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level) in total Cr concentration among soil cores sampled and among  soil
depth was found (Table  33).   The average total Cr content in the soil
profile of the sewage farm changed 30.1 percent in Rioconcho soil, 65.7
percent in Angelo soil  and 83.2  percent in Mereta soil after 18  years of
sewage wastewater irrigation (Table 37).   The average increase of total
Cr content of all soils in the top 20 cm was 59.7 percent.

Copper

     Copper  (Cu) is  essential to plant growth and has a normal concentration
in plant tissue ranging from 5 to 20 ppm (Walsh et al.  1976).  The con-
centration of Cu in  soils  ranges from 2 to 100 ppm with an average of 20
ppm (Haan and Zwerman 19 J6.).   In neutral and alkaline soils  Cu exists in
soil solution as the Cu   ,  and Cu(OH)  ions.   The Cu in  soils may originate
from fertilizer, manure, mine effluents,  industrial and sewage wastes, and
soil parent materials.   Copper sulfate has been used as either a fungicide
for fruit crops and  potatoes or as an additive in swine and  poultry feed.
The mobility of Cu in soils  is low and Cu tends to accumulate  in the top-
soil.  Jenne (1968)  postulated that the hydrous oxides  of Mn and Fe control
the concentration of Cu, Ni,  and Zn in soils and water.   Stevenson (1977)
studied the nature of the  binding of divalent metal ions  by  soil humic acid.
He showed the stability constant of metals in soils followed the order
Cu>Pb»Cd>Zn.

     The distribution of total Cu with soil depth in the  sewage  farm soils
and the control area soils is shown in Figure 19.   In Mereta soils in the
sewage farm, an accumulation of Cu in the 0-5 cm of top soil was observed.
This is a significant increase (at the 10 percent level)  in  total Cu con-
centration between the  sewage farm soils and the control  area  soils  (Table
33 ).  Irrigation with wastewater on the sewage farm increased the average total
Cu content in the upper 20 cm soil solum in Rioconcho soils, Angelo soils,
and Mereta soils by  16.8,  22.9,  and 62.3 percent,  respectively.   This
amounts to an average of 34.7 percent increase of total Cu content in
these three  soil series (Table 37 )•

Lead

     Lead (Pb) is a  biologically nonessential heavy metal.   The  total Pb
concentration of soils  ranges from 2 to 200 ppm with an average  of about
10 ppm (Allaway 1968).   The sources of Pb in soils include pesticides,
insecticides, fertilizer impurities, mining and smelting  operations,
engine exhausts,,and industrial and sewage wastes.  One of the potential
hazards associated with Pb contamination of soil is animal ingestion of
plants with abnormally  high concentrations of Pb, which may  be toxic to
the animal.  Lagerwerff and Specht (1970) reported that Pb concentrations
reached as high as 24,000  ppm in soils located directly along  a  roadside
in the Los Angeles metropolitan area.  Singer and Hanson  (1969)  studied
the Pb accumulation  in  soils near highways and found that PbC03> Pb»(PO^),,
and PbSO. were the insoluble Pb compounds formed in the soil.  Walsfi et -al.
(1976) suggested that in calcareous soils in the arid and semi-arid  region,
lead carbonate may form and the calcareous soil would then act as a  sink
for applied Pb.


                                      105

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             RIOCONCHO
     ANGELO
MERETA
DEPTH
  cm
                1.0  1.5  2,0  2$
 TOTAL-Cu,>ug/g

0  $   Ip  1.5  2.0 2.5
 IP
                            dlSEWAGE FARM SOIL

                                CONTROL SOIL
         Figure 19.  The effect of sewage effluent irrigation on total soil copper

   content of the top 200 cm of soil taken from the sewage farm compared to the control area.

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     The distribution of total Pb with  soil  depth in the  sewage  farm and the
control area is shown in Figure  20.   An accumulation of total Pb in the
upper 50 cm of soil in Mereta soils  at  the sewage farm was  observed.  There
is a significant difference at the 1 percent level for total Pb  concentra-
tion among three soil series and among  soil  cores sampled (Table 33)
Irrigation of wastewater on the  sewage  farm  significantly increased (at the 1
percent level) the total lead concentration  in  the three  soil series.  The
average increase in total Pb content in soil profiles  of  the sewage farm are
0.0, 130.9 and 1614.6 percent in Rioconcho,  Angelo,  and Mereta soils,
respectively (Table 37).  The average increase  of total Pb  content of these
three soil series is 507.1 percent after 18  years of wastewater  irrigation.

Nickel

     To date nickel (Ni) has no  essential function in  plant growth.  It is
readily absorbed by plants.  The total  Ni concentration of  soil may vary
from 5-500 ppm, with mean values commonly around  40  ppm (Allaway  1968).
Most of the Ni in soils comes from weathering products of basic rocks,
impurities in superphosphate fertilizer, automobile  exhausts, and industrial
and sewage wastes.  Nickel is considered 8 times  as  toxic as Zn to plant
growth in the calculation of the "Zn equivalent factor" (Chumbley 1971).
The U. S. Environmental Protection Agency suggests that Ni  is 4 times as
toxic to plants as Zn in the calculation of  their "metal equivalent".
Hinesly et al. (1972) found 42 to 56 percent of the  Ni applied as sewage
sludge is retained in the surface 15 cm of- soil.   Anderson and Nilsson
(1972) showed a 140 percent recovery of Ni in the surface 20 cm of soil
treated with 84 metric tons of sewage sludge over a  period of 12 years.

     The distribution of total Ni with  soil  depth in the sewage farm soils
and the control area soils is shown  in  Figure 21.  Generally, the total Ni
concentration increased with increased  soil  depth, and reached a maximum
at the 50-100 cm depth.  There is a  significant difference  (at the 1
percent level) in total Ni concentration among  three soil series, among
soil cores sampled, among soil depth, and between the  sewage farm soils
and the control area soils (Table 33).   Irrigation of  sewage wastewater
over a period of 18 years increased  the average total Ni content in the
upper 20 cm Angelo soil solutn by 12.3 percent (Table 37) .   In Rioconcho
soils and Mereta soils a decrease of 8.0 to  12.3  percent of average total
Ni content in the upper 20 cm soil solum was  observed.  This amounts to a
decrease of 2.8 percent of total Ni  content  of  these tnree  soil  series.

Zinc

     Zinc (Zn) is an essential element  for both plants and  animals.  It is
an important component of a number of enzyme systems.  Zinc reaches the
soils in various chemicals and wastes.   The  total Zn concentration of
soils under normal conditions ranges from 10-300  ppm,  with  an average
value of 50 ppm (Allaway 1968).  Lindsay (1973) showed that the  predominant
Zn series in soils below pH 7.7  is Zn   and  at  higher  pH  values  is Zn(OH)  .
Toxic Zn symptoms usually start  at about 400 ppm  and up for most plant
species.  If the Zn content of the animal diet  exceeds about 1000 ppm, Zn
toxicity will occur in animals (Haan and Zwerman  1976).   Chaney  (1973)


                                     107

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                   RIOCONCHO
                                      ANGELO
                           MERETA
o
oo
DEPTH
  cm
    0-5
    5-10
  10-15
  15-20
  20-30
  30-40
  40-50
 50- 100
100-150
150-200
                  1.0  2.0 3.0  4.0  5.0
TOTAL Pb,>ug/g
10  20 30 40  5.0
1.0  20 3.0  4.0 50
        /I07.22J
                                 C3SEWAGE  FARM SOIL
                                 • CONTROL SOIL
                 Figure 20.  The effect of sewage effluent irrigation on total soil lead
         content of the top 200 cm of soil taken from the sewage farm compared to the control area.

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                    RIOCONCHO
      AN6ELO
MERETA
       DEPTH
         cm
o
VO
                              2025
TOTAL-Ni, xtg/g
0   5   10 15  20  25
 10  15   20 25
                                       SEWAGE FARM SOIL
                                       CONTROL  SOIL
                Figure 2J..  The effect of sewage effluent irrigation on total soil nickel
          content of the top  200 cm of soil taken from the sewage farm compared to the control area.

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recommended that the toxic metal addition should not exceed Zn equivalent
levels equal to 5 percent of the CEC at p'H<6.5.  Decreasing the soil pH will
increase the total Zn concentration and its availability to plants.
Cunningham (1975).found that enhancement of soil total Zn to 1,300 ppm by
sludge treatment would cause plant Zn concentrations in excess of 900 ppm
and result in severe reduction of yield.

     The distribution of total Zn concentration with soil depth in the
sewage farm soils and the control area soils is shown in Figure 22.  There
is an accumulation of total Zn in the upper 10 cm soil in Mereta soil series.
Irrigation with sewage wastewater does not change the Zn concentration of
soils in the sewage farm (Table 33).  There is a significant difference in
total Zn concentration among the three soil series, among soil cores
sampled, and among soil depths.  The differences in total Zn content between
the sewage farm and the control area in the upper 20 cm soil profile of
Rioconcho, Angelo, and Mereta soils are -4.9, +45.6, and H-199.2 percent,
respectively.  Irrigation of wastewater increases 81.2 percent of total Zn
content in the top 20 cm soil profile of these soil series of the sewage
farm.
                                     110

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             RIOCONCHO
            ANGELO
                               MERETA
DEPTH
  cm   0
100
  TOTAL- Zn,>qg/g
0        50       100
100
                            QSEWAGE  FARM SOIL
                            • CONTROL SOIL
          Figure 22.  The effect of sewage effluent irrigation on total soil zinc
   content of the top 200 cm of soil taken from the sewage farm compared to the control area.

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                             SECTION  7

                            REFERENCES
Agboola, A. A., and R. B. Corey.  The Relationship Between Soil
pH, Organic Matter, Available Phosphorus, Exchangeable Potassium,
Calcium, Magnesium, and Nine Elements in the Maize Tissue.  Soil
Science, 115:367-375, 1973.

Allaway, W. H.  Agronomic Controls Over Environmental Cycling of
Trace Elements.  In N. C. Brady ed. Advances in Agronomy, Academic
Press, N.Y., N.Y., 20:235-274, 1968.

Allison, L. E., and C. D. Moodie.  Carbonate.  In:  Methods of
Soil Analysis, part 1, ed. by C. A. Black et al., American Society
of Agronomy, Madison, Wis., 1965.  pp. 1387-1388.

American Public  Health Association, American Water Works Association,
Water Pollution Control Federation.  Standard Methods for Examination
of Water and Wastevrater. 14th ed., Washington, B.C., 1976.  900 pp.

Anderson, A. and K. 0. Nilsson.  Enrichment of Trace Elements from
Sewage Sludge Fertilizer in Soils and Plants.  Ambio 1:176-179, 1972.

Barnes, V. E.  Geologic Atlas of Texas, San Angelo Sheet.  Bureau
of Economic Geology, The University of Texas at Austin, 1974.

Barr, A. J., J. H. Goodnight, J. P. Sail, J. T. Helwig.  A User's
Guide to SAS - 76.  SAS Institute, Inc., Raleigh, North Carolina, 1976.

Bartlett, R. J., and J. M. Kimble.  Behavior of Chromium in Soils:
II Hexavalent Forms.  J. Environ. Qual.  5:383-386, 1976.

Beek, J.y F.,A. M. de Haan, and W. H. van Riemsdijk.  Phosphates in
Soils Treated with Sewage Water:  1.  General Information on Sewage
Farm, Soil, and Treatment Results.  J. Environ. Qual., 6:4-7, 1977.

Blake, G. R.  Bulk Density.  In:  Methods of Soil Analysis, part 1,
ed. by C. A. Black et al., American Society of Agronomy, Madison,
Wis., 1965.  pp. 374-390.

Bouwer, H., and R. L. Chaney.  Land Treatment of Wastewater.  In:
N. C. Brady ed. Advances in Agronomy, Vol. 26, p. 133-176, Academic
press, New York, N.Y.  1973.
                                 112

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Bower, C. A., and L. V. Wilcox.   Soluble Salts.   In:   Methods of
Soil Analysis, part  2,  ed.  by C.  A.  Black et al.  American Society
of Agronomy, Madison, Wis.,  1965a.   pp.  933-951.

Bremner, J. M.  Total Nitrogen.   In:   Methods of  Soil Analysis, part
1, ed. by C. A. Black et  al.   American Society of Agronomy, Madison,
Wis., 1965a.  pp. 1149-1178.

Bremner, J. M.  Inorganic Forms of Nitrogen.   In:   Methods of Soil
Analysis, part 2, ed. by  C.  A. Black et  al.   American Society of
Agronomy, Madison, Wis.,  1965b.   pp.  1179-1237.

Buchauer, M. J.  Contamination of Soil and Vegetation Near a  Zinc
Smelter by Zinc, Cadmium, Copper  and Lead.   Envir.  Sci.  Technol.
7:31, 1973.

Cairo, J. H.  Characterization of  Super Phosphate.   In:   Superphosphate:
Its History, Chemistry  and  Manufacture.   Agri. Res. Serv., U.  S.
Dept. of Agri., Washington,  D. C., 1964.

Chaney, R. L.  Crop  and Food Chain Effects of Toxic Elements  in
Sludges and Effluents.  In:   Recycling Municipal  Sludges and  Effluents
on Land, Champaign,  111., 1973.   pp.  129-141.

Chumbley, C. G.  Permissible Levels  of Toxic  Metals in Sewage used on
Agricultural Land.   A.  D. A.  S. Advis. paper  10,  1971.   12 pp.

Cunningham, J. D., D. R.  Kenney,  and J.  A. Ryan.   Phytoxicity and
Uptake of Metals Added  to Soils as Inorganic  Salts or in Sewage
Sludge.  J. Environ. Qual.   4: 460-462,  1975.

Day, A. D., and R. M. Kirkpatrick.   Effects of Treated Municipal
Wastewater on Oat Forage  and Grain.   J.  Environ.  Qual.,  2: 282-284, 1973.

Day, P. R.  Particle Fractionation and Particle-size  Analysis.  In:
Methods of Soil Analysis, part 1, ed.  C.  A. Black et  al., American
Society of Agronomy, Madison,  Wis.,  1965.  pp. 545-567.

de Haan, F. A. M., and  P. J.  Zwerman.  Pollution  of Soil.  In:
Soil Chemistry, A Basic Elements, ed.  by G. H. Bolt and  M. G.  M.
Bruggenwert, Elsevier Sci.  Publ.  Comp.,  N. Y., 1976.   pp.  192-271.

Dowdy, R. H. and W.  E.  Larson.  Metal Uptake  by Barley Seedlings
Grown on Soils Amended  with Sewage Sludge.   J. Environ.  Qual.
4: 229-233, 1975.

Ellis, B. G.  The Soil  as a Chemical Filter.   In:   Conference on
Recycling Treated Municipal Waste Water  Through Forest and Cropland,
ed. by Sopper, W. E. and  L.  T. Kardos.  EPA-600/2-74-003. U. S.
Environmental Protection  Agency,  Washington,  D. C., 1974. pp. 47-72.
                                 113

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Frere, M. H.  Nutrient Aspects  of  Pollution from Cropland.   In:
Control of Water Pollution  from Cropland,  Volume II-An overview.   U.  S.
Environmental Protection Agency, Washington,  D.  C.,  1976.   pp.  59-90.

Fuller, W. H., N. E. Korte, E.  E.  Niebla,  and B.  A.  Alesii.   Contribu-
tion of the Soil to the Migration  of  Certain Common  and Trace Elements.
Soil Sci.  122:223-235, 1976.

Giordano, P. M., and J. J.  Mortvedt.  Nitrogen Effects on Mobility
and Plant Uptake of Heavy Metals in Sewage Sludge Applied to Soil
Column.  J. Environ. Qual.  5:165-168,  1976.

Griffin, R. A. and N. F. Shimp.  Leachate  Migration  Through Selected
Clays.  In:  Gas and Leachate from Land Fills:   Formation,  Collection,
and Traetment.  ed. by Genetelli,  E.  J. and J. Cirello,  EPA-600/9-76-
004, U. S. Environmental Protection Agency,  Cincinnati,  Ohio, 1976.
pp. 92-95.

Hagiri, F.  Plant Uptake of Cadmium as  Influenced by Cation  Exchange
Capacity, Organic Matter, Zinc, and Soil Temperature.   J. Environ.
Qual.  3:180-183, 1974.

Hinesly, T. D., R. L. Jones and B. Sosewitz.   Use of Waste  Treatment
Plant Solids for Mined Land Reclcmation.   Mining  Congress Journal.
58:66-73, 1972.

Hodgson, J. F.  In Trace Substances in  Environmental Health.  Ed.  by
D. D. Hemphill, Vol. Ill, University  of Missouri  Press,  Columbia,  Mo.,
1970.  pp. 45-58.

Jackson, M. L.  Soil Chemical Analysis.  Presntice-Hall  Inc., Englewood
Cliffs, New Jersey., 1958.  507 pp.

Jenne, E. A.  Control of Mn, Fe, Co,  Ni, Cu,  and  Zn  Concentrations in
Soils and Water:  The Significant  Role  of  Hydrous Mn and Fe  Oxides.
In:  Trace Inorganic in Water, Advances in Chem.  Series, Am.  Chem.
Soc., Washington, D. C., 73:337-387,  1968.

John, M. K.  Influence of Soil Properties  and Extractable Zinc on
Zinc Availability.  Soil Sci. 113:222-227,  1972.

John, M. K., C. J. Van Laerhoven,  and H. H.  Chuah.   Factors  Affecting
Plant Uptake and Phytotoxicity of  Cadmium  Added  to Soils.  Environ.
Sci. Techn. 6:1005-1009, 1972.                                    ,:,

Johnson, J. W., L. F. Welch, and L. T.  Kurtz.  Environmental
Implications of N Fixation  by Soybean.  J.  Environ.  Qual. 4:303-306,
1975.

Kao, C.  W., and R. W. Blanchar.  Distribution and Chemistry  of
Phosphorus in an Albaqualf  Soil After 82 years of Phosphate  Fertiliza-
tion.  J.  Environ. Qual. 2:237-240, 1973.


                                 114

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Reefer, R. F., and R. Estepp.   The  Fate  of  Zinc-65 Applied to Two
Soils as  Zinc Sulfate and  Zinc-EDTA.  Soil Sci.  112:325-329, 1971.
Keeney, D. R., and J. M. Bremner.  Determination of Soil Cation
Exchange Capacity 1
107:334-336,  1969.
Exchange Capacity by^ a Simple Semimicro Technique.  Soil Sci.
Korte, N. E., J. Skopp, W. H. Fuller, E. E. Niebla, and B. A. Alesii.
Trace Element Movement in Soils:   Influence of Soil Physical and
Chemical Properties.  Soil Sci.   122:350-359, 1976.

Kreitler, C. W. Determining  the Source of Nitrate in Ground Water
by Nitrogen Isotope Studies.  Report of Investigation no. 83,
Bureau of Economic Geology,  The University of Texas at Austin,
Austin, Texas,  1975.  62 pp.

Lagerwerff, J.  V.  Uptake of Cadmium, Lead and Zinc by Radish from
Soil and Air.   Soil Sci., 111:129-133, 1971.

Lagerwerff, J.  V., and A. W. Specht.  Contamination of Roadside
Soil and Vegetation with Cadmium,  Nickel, Lead, and Zinc.
Environ. Sci. Tech.  4:583-586, 1970.

Lance, J. C. Nitrogen Removal by  Soil Mechanisms.  J. Water Pollut.
Contr. Fed.  44:1352-1361, 1972.

Lindsay, W. L.  Inorganic Reactions of Sewage Wastes with Soils.
In:  Proc. Joint Conf. Recycling  Municipal Sludges and Effluents
on Land.  Sponsored by EPA,  U.S.D.A. and NASULGC, Champaign, 111.,
1973.  pp.  91-96.

Lindsay, W. L., and E. C. Moreno.  Phosphate Phase Equilibrium
Soils.  Soil Sci. Soc. Amer. Proc.  24:177-182, 1960.

Lisk, D. J.  Land Disposal of Sewage Sludge Termed Hazardous.
Environ. Sci. Techn. 6:397,  1972.

Lykins, B. W. Jr., and J. M. Smith.  Interim Report on the Impact
of Public Law 92-500 on Municipal Pollution Control Technology.
EPA-600/2-76-018.  U. S. Environmental Protection Agency,
Cincinnati, Ohio, 1976.  320 pp.

Macias, F. D.   Copper and Zinc Status in Pasture Soils of Salamanca,
Spain.  Soil Sci.   115:276-283, 1973.

MacKenthun, K.  M.  Toward a  Cleaner Aquatic Environment.  Office of
Air and Water Programs, U.S. Environmental Protection Agency,
Washington, D.C., 1973.  290 pp.

Martens, D. C.  Plant Availability of Extractable Boron, Copper,
and Zinc as Related to Selected Soil Properties.  Soil Sci.
106:  23-28, 1968.

                                115

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Melsted,  S. W.  Soil-plant  Relationships  (Some Practical  Consid-
erations  in Waste Management),  In:   Proc.  Joint.  Conf. on
Recycling Municipal  sludges and Effluents  on  Land,  Sponsored by
EPA, U.S.D.A.,  NASULGC,  Champaign,  Illinois,  1973.   pp.   121-128.

Miller, J. E.,  J. J. Hassett, and D. E.  Koeppe.   Uptake  of  Cadmium
by  Soybeans as  Influenced  by Soil Cation Exchange Capacity,  pH,
and Available Phosphorous.  J.  Environ.  Qual.  5:157-160,  1976.

Murphy, J. and  J. Riley.   A Modified Single Solution for the
Determination of Phosphate in Natural Waters.  Anal.  Chem.  Acts
27:31,  1962.

National  Academy of  Sciences-National Academy of  Engineering.
Water Quality Criteria,  1972.   U.S.  Government Printing  Office,
Washington, B.C., 1973.  613 pp.

.National  Technical Advisory Committee to the  Secretary of  the
Interior. Water Quality Criteria.   Federal Water Pollution
Control Administration,  Washington,  D.C.,  1968.   244 pp.

Olsen,  S. R. and L.  A. Dean.  Phosphorus.  In:  Methods  of  Soil
Analysis, part  2,'ed. by C. A.  Black et  al.,  American Society of
Agronomy, Madison, Wis., 1965.   pp.  1035-1049.

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Traces  of Zinc  in Phosphate Fertilizers.   Aust. J.  Exp.  Agri.
Anim. Husb. 5:29-33, 1975.

Page, A.  L., F. T. Bingham, and C.  Nelson.  Cadmium Absorption and
Growth  of Various Plant  Species as  Influenced by  Solution Cadmium
Concentration.  J. Environ. Qual. 1:288-291,  1972.

Peech,  M. Hydrogen-ion Activity.  In:  Methods of Soil Analysis,
part 2, ed. by  C. A. Black et al. American Society  of Agronomy,
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Sect. AY-5.,  1971.   pp.  1-2.

Prasad, B., M.  K. Sinha, and N.  S.  Randhawa.   Effect of  Mobile
Chelating Agents on  Diffusion of Zinc in Soils.   Soil Sci.,
122:260-266,  1976.

Reneau, R. B. Jr. Changes  in Inorganic Nitrogenous  Compounds from
Septic  Tank Effluent in  a  Soil  with a Fluctuating Water  Table.
J.  Environ. Qual.  6:173-178, 1977.
                                116

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Le Riche, H. H.  Metal  Contamination of  Soil  in the Woburn Market
Garden Experiment Resulting  from the Application of Sewage Sludge.
J. Agr. Sci. Cam.,  71:205-208,  1968.

Singer, M. J. and L. Hanson.  Lead Accumlation  in Soils near
Highways in the Twin Cities  Metropolitan Area.   Soil Sci. Soc.
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Stanford, C. T.  Rationale for  Optimum Nitrogen Fertilization in
Corn Production.  J. Environ. Qual.   2:159-166,  1973.

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Fertilizer on Denitrification from Four  South Australian Soils.
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Stevenson, F. J.  Nature  of  Divalent Transition Metal Complexes
of Humic Acids as Revealed by a Modified Potentiometric Titration
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                                117

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Walsh, L. M., M. E. Stunner, and R. B. Corey.  Consideration of Soils
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101 pp.
                                 118

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                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before completing)
 REPORT NO.
    EPA-600/2-78-131a
2.
                              3. RECIPIENT'S ACCESSION-NO.
 TITLE AND SUBTITLE
     SEWAGE DISPOSAL  ON AGRICULTURAL  SOILS:   CHEMICAL
     AND MICROBIOLOGICAL IMPLICATIONS  (VOLUME I CHEMICAL
     IMPLICATIONS)
                              5. REPORT DATE
                                June 1978 issuing date^
                              6. PERFORMING ORGANIZATION CODE
 AUTHORCS)
           L> R_ Hossner
           Chun-Wei  Kao
           R. W. Weaver
   J.  A. Waggoner
                              8. PERFORMING ORGANIZATION REPORT NO,
 PERFORMING ORGANIZATION NAME AND ADDRESS
     Texas A&M University
     Department of  Soil & Crop Sciences
     College Station, Texas  77843
                              10. PROGRAM ELEMENT NO.
                                    1BC611
                              11. CONTRACT/GRANT NO.

                                    R803281
12. SPONSORING AGENCY NAME AND ADDRESS
    Robert  S.  Kerr Environmental Research Lab.
    Office  of  Research and Development
    U.S.  Environmental Protection Agency
    Ada,  Oklahoma  74820
                                                  - ADA,  OK
                              13. TYPE OF REPORT AND PERIOD COVERED
                                 Final - 1975-1977
                                                            14. SPONSORING AGENCY CODE

                                                              EPA/600/15
15. SUPPLEMENTARY NOTES
 16. ABSTRACT
   The  city of San Angelo,  Texas, has used sewage effluent which has undergone
   primary treatment for  irrigation of the same  259-hectare sewage farm since 1958.
   The  impact of 18 years of sewage effluent  irrigation on the soil and water quality
   was  studied from 1975  to 1977.  The volume of sewage arriving at the sewage  treat-
   ment plant ranged from 0.219 to 0.351 m^/sec.  and contributed from 3.4 to 31.2
   percent of the total flow of the bordering Concho River in 1976.  'The fate of
   applied nitrogen, phosphorus, and selected heavy metals in the soil-water-plant
   ecosystem were examined.  There was evidence  of some NO-j-N accumulation in the
   soil profile and in the Concho River while no accumulation of NH^ -N was found
   in the soil profiles.   Some organic nitrogen  accumulated at the soil surface,
   but  after 18 years of  operation there was  a net loss of total nitrogen in the
   soil profile.  Phosphorus accumulated in the  soil profile to depths of 200 cm
   along with a slight build-up of certain heavy metals in the surface soils.   Deep
   wells appeared to be uncontaminated by the sewage farm operation.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                              b.lDENTIFIERS/OPEN ENDED TERMS
                                                                         c. COS AT I Field/Group
  Land  use
  Sewage treatment
  Nutrient
  Removal
                                              Land pollution abatement
                                              San Angelo,  Texas
                                              Land application
                                              Municipal  wastewater
                                              Rural land use
                                              Farmlands
                                              Sewage effluents
                                                    pffliipnt-  t-ommral
                                              68D
                                              91A
                                              43F
18. DISTRIBUTION STATEMENT
   RELEASE TO PUBLIC
EPA Form 2220-1 (9-73)
                                               19. SECURITY CLASS (ThisReport)
                                                131
                                               20. SECURITY CLASS (Thispage)
                                                      UNCLASSIFII
                                                                          22. PRICE
                                                                      ft U.S. OOVBNMENTPMHTlhG OfFTCL 1978—757 -140/1356

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