&EPA
             United States
             Environmental Protection
             Agency
             Environmental Sciences Research
             Laboratory
             Research Triangle Park NC 27711
EPA-600/2-78-197
September 1978
             Research and Development
Calibration Standards for
X-ray Spectrometers
Used for Pollution
Sample Analysis

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                RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology  Elimination of traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:

      1.  Environmental Health  Effects Research
      2.  Environmental Protection Technology
      3.  Ecological Research
      4.  Environmental Monitoring
      5.  Socioeconomic Environmental Studies
      6.  Scientific and Technical Assessment Reports (STAR)
      7.  Interagency Energy-Environment Research and Development
      8.  "Special" Reports
      9.  Miscellaneous Reports

This report has  been assigned  to the ENVIRONMENTAL PROTECTION TECH-
NOLOGY series. This series describes research performed to develop and dem-
onstrate instrumentation, equipment, and methodology to repair or prevent en-
vironmental degradation from point and non-point sources of pollution. This work
provides the new or improved technology required for the control and treatment
of pollution sources to meet environmental quality standards.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                               EPA-600/2-78-197
                                               September 1978
  CALIBRATION STANDARDS FOR X-RAY SPECTROMETERS
       USED FOR POLLUTION SAMPLE ANALYSIS
                      by

    Richard A.  Semmler and Ronald G.  Draftz
            NT Research Institute
                10 W.  35th St.
           Chicago, Illinois  60616
            Contract No.  68-02-173^
                Project Officer

                  Roy Bennett
Emission Measurement and Characterization Division
    Environmental  Sciences Research Laboratory
   Research Triangle Park, North Carolina  277H
   ENVIRONMENTAL SCIENCES RESEARCH LABORATORY
       OFFICE OF RESEARCH AND DEVELOPMENT
      U.S. ENVIRONMENTAL PROTECTION AGENCY
  RESEARCH TRIANGLE PARK, NORTH CAROLINA  27711

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                                DISCLAIMER

     This report has been reviewed by the Environmental  Sciences Research
Laboratory, U.S. Environmental  Protection Agency,  and approved for
publication.  Approval  does not signify that the contents necessarily
reflect the views and policies  of the U.S. Environmental  Protection Agency,
nor does mention of trade names or commercial  products constitute
endorsement or recommendation for use.
                                      i i

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                                 ABSTRACT

     A technique is described for making aerosol standards for x-ray
fluorescence analysis by depositing micron sized particles suspended in a
carrier solution onto the surface of a Nuclepore filter.  Size is controlled
by a separate sedimentation step following grinding in a boron carbide
mortar and pestle.  Binding of the deposition to the filter uses a collodion
film layer applied both before and after the particle deposition.  The
deposited mass is determined gravimetrically from a companion filter prepared
from a large volume of carrier solution and without collodion.  Standards
for 24 different elements have been prepared.
                                     i i i

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                                  CONTENTS
Abstract	iii
Figures	vi
Tables	vii
Acknowledgments	viii

     1.   Introduction 	  1
     2.   Recommendations and Conclusions  	  2
     3.   Procedure for Standards Preparation  	 .  	  3
               Preparing the suspension  	  3
               Filtering and coating the filters ... 	  4
               Mounting the completed filters  	  5
               Packaging the completed filters 	  6
     5.   Development of a Preparation Technique 	  	  7
               Preparation of suspensions  	  7
               Filtering the suspensions 	  7
     6.   Completed Standards	12
               Characterization of completed standards .	12
               Materials selection and target values	27

References	33
Appendices

     A.   Self absorption in a sphere	34
     B.   Serial number lists	38

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                                   FIGURES
Number


  1    Filter apparatus for 20 mm and 35 mm deposits prepared 	   9
         during Phase 1

  2    Filter apparatus for 35mm.deposits prepared during Phase 2  .  .  10

  3    Probability of escape from a uniform spherical source as a
         function of diameter/mean-free-path  ... 	  28

  4    Probability of escape from a uniform spherical source as a
         function of diameter and mean free path	29

  5    Geometry and notation used for describing escape of x-ray.
         from within a spherical particle .	36
                                     VI

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                                   TABLES
Number                                                                  Page





  1    Calibration Data for Stock Suspension  	 13




  2    Surface Density Calculation for Heaviest 35 mm Standard  	 15




  3    Elemental Deposits on the Supplied Aerosol Standards   	 17




  4    Analysis Data for .Compounds Used in Delivered Standards	19




  5    Particle Size Data for Light Elements	24




  6    X-ray .Characteristics for Elements and Compounds of Interest   .  . 25




  7    Chemical Forms Selected for Use in Deposited Particulates  .... 30




  8    Desired Density and Other Data for Compounds of Interest   .... 31




  9    Desired Density and Other Data for Compounds of Interest   ....  32
                                      VI1

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                               ACKNOWLEDGMENTS
     The invaluable assistance  of Joe  Puretz  and  George  Yamate for the
laboratory work is  gratefully appreciated.
                                   Vlll

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                                 SECTION 1

                               INTRODUCTION
     The need for reliable calibration standards is often not appreciated
until considerable time and effort has been expended on instrument
development.  X-Ray standards are no different and each investigator has
typically created his own special reference materials.  This program to
prepare x-ray standards has concentrated on the development of a technique
which could be used with a variety of compounds to produce thin layers of
finely divided particles on the surface of the supporting substrate so that
particle size effects, interelement effects, and substrate absorption would
be minimal.  The final technique which has been adopted is deposition of
previously sized particles from a suspension in isopropyl alcohol  (IPA) onto
a Nuclepore filter plus a collodion film binder.  Other binder techniques
have been attempted,  in particular, softening of the Nuclepore filter, but
these have proved unreliable  in practice.  Details of the final procedure
are given  in Section  3-  A discussion of the evolution of the procedure is
given in Section 4.

     Information on the specific compounds used for the standards is
collected within Section 5-   This includes available  information on purity,
gravimetric data on mass depositions, and comments on the effects of
particle size.

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                                 SECTION 2

                      RECOMMENDATIONS AND CONCLUSIONS
                                                             f

     A successful deposition technique for the preparation of thin aerosol
standards has been defined.

     The essential steps include (1) grinding and sedimentation to control
particle size to  1 micron and under, (2) suspension of particles  in  isopropyl
alcohol, (3) filtration of a large volume of suspension to gravimetrically
determine deposition rate, (4) deposition of a collodion film on  the
Nuclepore substrate prior to particle deposition, (5) particle deposition,
and  (6) deposition of a second collodion film to complete the binding.
Essential details of the steps are described in other sections.

     The problems encountered while developing the technique have been more
severe  than  anticipated, however, and the final procedure itself  is more
complex and  time consuming than the original estimates.  Roughly, one should
allow a man-week of effort for the production of 1 set of standards, i.e.,
a  total of  6 filters of one element with 2 deposition diameters and 3
different  thicknesses.  Proficiency and development of parallel processing may
eventually  alter the time  but experience has indicated that initially one
third of the time will be  sent on grinding, sedimentation, sizing, and
calibration; one third of  the time on dilution, deposition, and mounting
of the  35  mm deposits; and one third of the time on dilution, deposition,
and mounting of the 20 mm  deposits.  Any additional characterization such as
microscopic measurements on size and uniformity requires additional time.

      While grinding and suspension of each new compound presents  a new
 situation  and  possible problems, the procedure is now relatively  straight-
 forward.   Two  areas for potential problems should be kept in mind, however.
 One is  the filter holder design.  We have found variations in manufacture
 of the  supplied holders which cause wrinkling of the completed filter in the
 process of closing  the holder.  This can ruin a standard after the investment
 of considerable time and effort.  The other area  is compatibility with the
 isopropyl  alcohol used for suspension.   If a change  in liquids is required,
 the deposition characteristics may  be altered requiring a modification of the
 procedure.

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                                 SECTION 3

                    PROCEDURE FOR STANDARDS PREPARATION


     The current procedure for producing calibration standards can best be
described by dividing the entire process into four parts:

     A.  Preparing the Suspension

     B.  Filtering and Coating the Filters

     C.  Mounting the Completed Filters in Permanent Retainers

     D.  Packaging the Completed Filters

     The advantages and disadvantages of the current procedure and its
evaluation will be discussed  in Section 4.

PREPARING THE SUSPENSION

     Preparing  suspensions  involved essentially six  (6) phases:   (1) Micro-
scopical examination of pure  materials, (2) grinding,  (3) weighing^
(4) dispersal by sonification,  (5) sedimentation, and  (6) reweighing.

     1.  Powders are examined by optical microscopy  to determine whether
         the particle size  is suitable for immediate filtration or whether
         grinding  is necessary.

     2.  When grinding  is necessary, the powders are ground  in a boron
         carbide mortar and pestle for approximately 20 minutes and then
         transferred to a clean vial.

     3.  One hundred milligrams  (100 mg) of the ground sample  is weighed
         on an  analytical balance and transferred to a clean 150 ml beaker.
         Approximately  100 mis of  I PA is added to the  beaker.

     k.  The beaker containing the suspension  is placed  in an  ultrasonic
         bath for 20 minutes  to break up weakly bound  agglomerates.  The
         suspension is then cooled to room temperature and particles are
         allowed to settle for 20 minutes.  This cycle is repeated 3 or k
         times to de-agglomerate the suspension as completely as possible.

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     5.    The  suspension  is  then  allowed  to  settle for 20 minutes  to
          remove  the  large particles.   The fines  are then carefully
          decanted  into a 1000 ml  volumetric flask and brought to  volume
          with IPA.   Most of the  liquid is decanted leaving only the
          obvious sediment at the bottom.

     6.    The  coarse  fraction is  then  filtered  through a pre-weighed
          Nuclepore membrane.  After drying, the  filter is reweighed.
          The  difference  in  weight between the  original  100 mg and the
          coarse  fraction now on  the filter  gives the approximate  weight
          of fines.

FILTERING THE  SUSPENSIONS

     Filtering suspensions  involved (1) dilution, (2) filtration apparatus
set-up, (3) filtration and  coating, (4) drying, and (5)  mass concentration
determination.  The first four steps are  performed in a clean bench.
                                       *.
     1.   The approximate mass concentration of fines is used to estimate the
          suspension  volume  required to produce depositions in the desired
          range.   Two different techniques were used to vary the mass  being
          deposited.   In  Method I, the fines are  first diluted to  produce
          the suspension  volume needed to produce the heaviest desired standard.
          The second most concentrated standard is then prepared by diluting the
          first suspension  1:10.   The  least  concentrated standard  is prepared
          from a 1:10 dilution of the  second suspension.  The suspensions then
          have a concentration ratio of 100:10:1.  The same volume is  used for
          each suspension to prepare the standards, thus insuring  uniform
          precision.   The Method I dilution  technique was used during  Phase 1
          to prepare both the 20 mm and 30 mm standards, each size with 3
          different concentrations (the 30 mm standard actually has a  deposit
          over a 35 mm diameter).  The 20 mm standards were made using half the
          volume of suspension used for the  30  mm standard.  Subsequent doubts
          about the trace solubility of so-called "insoluble" compounds led
          to use of Method  II which completely  avoided multiple dilutions.
          Method II was used during the second  phase of the program when 6
          different deposits were prepared for  each compound using a 35 mm dia-
          meter and no 20 mm standards were  prepared.  In Method II, the fines
          are diluted to produce the total suspension volume needed for all
          depositions with  a concentration sufficiently dilute to permit use
          of at least 5 ml  of suspension for the  smallest deposit.

     2. ; The filter support and funnel are  thoroughly cleaned with IPA
          prior to filtration.  The preparation of a 30 mm standard requires
          an 0.8 ym Gelman  membrane filter as an  interface between the sintered
          metal support plant and the  0.4 urn Nuclepore membrane which
          receives the particles.  The Gelman filter produces a more
          uniform particle deposition.  The  two filters are rinsed in
          clean IPA and carefully positioned concentrically on the filter
          holder, Nuclepore on top, taking care that they are perfectly
          flat with no sign of wrinkling.  This is achieved by

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         simultaneously wetting the filter with I PA and applying a slight
         vacuum to the mounted filter.  The same procedure is used for the
         20 mm standard but no interfacial filter is required.

     3-   With a slight vacuum applied, the Nuclepore membrane is sprayed
         with a nebulized mist of 5% commercial collodion in amyl  acetate
         from  a DeVilbiss Glass Nebulizer AO.   The vacuum is gradually
         released and the filter funnel is carefully placed onto the
         mounted filter and clamped into place.  Some IPA is added to the
         empty filter assembly and then gradually the suspension is rinsed
         thoroughly and completely into the assembly.  A watch glass is
         placed over the funnel and vacuum is applied to the filter.
         During this stage of the process it is important to control
         the vacuum precisely, as too weak a vacuum will cause clogging
         of the filter and too strong a vacuum may cause non-uniform
         deposition, wrinkling or other problems.   Occasionally, the
         cover glass is removed and the funnel  wall  gently rinsed  with
         clean IPA to prevent sample loss, being careful not to disturb
         the suspension.  When the filtrate is  gone, revealing the
         substrate, the vacuum is made weaker,  the clamp is removed and
         the supper funnel is carefully lifted  off.   The filter is
         checked visually under a magnifying glass for uniformity  and
         boundary sharpness.   Vacuum is again applied and the filter
         is sprayed with a nebulized mist of 0.1% solution of collodion
         in amyl acetate.

     k.   The vacuum is released and the filter is placed on a flat
         square of Teflon and left to dry under a large, clean glass
         cover.

     5.   The exact mass concentration of fines  is determined by filtering
         a large volume of the most concentrated, suspension through a
         pre-weighed Nuclepore membrane.  The filter and residue are
         dried and reweighed to determine the precise weight of standard
         delivered to each filter standard.

MOUNTING THE COMPLETED FILTER

     The filter holders supplied by the EPA (1) are comprised of two parts:
(1) the  lower portion onto which the finished filter is placed and (2) the
upper retaining clip which holds the filter in place.

     The completed filter  is placed onto the bottom portion of the holder
and edge only is wetted carefully with a 0.11 collodion solution avoiding
the deposition entirely.  The filter is then manipulated until it lies flat
and virtually wrinkle free on the holder.

     Next a small crescent of Nuclepore membrane, onto which  is printed
(in ink) the element name, concentration ratio and deposit area size,  is
placed along the filter's edge, serving as a lavel.  The retaining clip  is

-------
compressed and placed over the filter and snapped into place in the bottom
holder.  This assembly is then permitted to dry under a watch glass In the
clean hood.

PACKAGING OF COMPLETED FILTERS

     Two clean 2" x 2" glass slides are assembled to form a sandwich into
which the standard is placed, thus serving to protect the upper and lower
surfaces of the filter.

DEPARTURES FROM STANDARD PROCEDURES

     Certain compounds required special  treatment.  Such problems as
solubility in I PA and distortion of the Nuclepore filter had to be overcome.

     Nad, Se02, As20. and KHCO, all  proved to.be soluble in IPA.  This
problem with NaCl was eliminatea by preparing a suspension using a
saturated solution of NaCl in water.

     The use of other solvents such as acetone was precluded because it
distorted the Nuclepore fiIter.

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                                 SECTION k

                   DEVELOPMENT OF A PREPARATION TECHNIQUE


     Initially a procedure for the preparation of the standards was attempted
according to a different work plan.  However, during the initial preparation
of filters, several unexpected problems arose.  These problems and our
solutions to them are listed below.

PREPARATION OF SUSPENSIONS

     Initially powders were selected on the basis of availability, purity,
and particle size according to the suppliers' specifications.   Under optical
microscopy, however, it was determined that many more powders  would require
grinding than was previously anticipated.  The pure metal powders
particularly fell outside the claimed size range.  Due to the  difficulty of
grinding metal 1ic. powders alternate sample selections were generally made.

     To avoid contamination In the grinding process a boron-carbide mortar
and pestle was used.  These low atomic number elements give no interference
for x-ray fluorescence studies.

FILTERING THE SUSPENSIONS

Pilution

     Early attempts at filter preparation involved the use of  volumetric
pipettes rather than graduated cylinders.  Pipettes seemed ideally suited
to the situation since small amounts of suspension could be withdrawn
accurately for the dilutions.  However, upon further consideration it was
realized that pipettes could not accurately nor economically be used for
these dilutions.

     Because only molecular "particle" sizes are encountered in a solution,
pipettes can be used accurately to deliver small quantities.  However,  in a
suspension, although mass concentration may be uniform,  particle sizes are
orders of magnitude larger and certain difficulties arise.

     A pipette is designed specifically so that  the delivered volume  is
correct when a small amount of liquid remains trapped  in the bottom as
implied by the designation TD  (to deliver).  This type pipette  should not be
blown out for accurate volume determination.  Consequently, the pipette can-
not be rinsed into the filtrate to remove particles adhering to the walls.

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     In order to avoid any cross contamination between suspensions, the
pipette had to undergo a thorough cleaning process  involving detergent
rinsing, distilled water rinsing, acid bath and distilled water rinsing
again.   The preparation of six filters per standard set and one additional
for mass determination rendering this method inaccurate and time consuming.

     As an alternative, graduated cylinders were used with a small sacrifice
in accuracy but with significant advantages due to ease in cleaning,  thereby
eliminating virtually all cross contamination in addition to the subsequent
saving of time.

Filtration System Set-Up

     The Delrin funnel shown in Figure 1 was used for both 35 mm and  20 mm
standards  (by  inverting the funnel) when Method I  was used to control mass
deposition.  The Delrin funnel in Figure 2 holds the larger volume required
for  the Method  II procedure of controlling mass deposition.

     As originally designed, the lower supports used specially fabricated
screens to support the filter under vacuum.  After several filters were
prepared  it was decided that they were unsatisfactory due to non-uniformity
of deposition  and indistinct outer boundary.  It was our  intention to
produce a  consistently uniform deposition with as sharp a boundary as
possible.  To  this end several modifications were made.  A sintered metal
backing plate  is used  in the 35 mm support and the original support screen for
the  20 mm  screen was modified to accommodate the screen in a slightly
recessed  position.

      In addition, for  the 35 mm standard,  it is necessary to use an 0.8 urn
Gelman  filter  underneath the Nuclepore membrane to assure a uniform filtration
surface and prevent the deposit from assuming the surface features of the
sintered  metal  backing plate.   It took many attempts to finally find  the
corrected  method for obtaining a uniform deposit.

Filtration and Coating

      Ideally,  our intention was to produce a high quality standard whose
surface could  be protected from dust and debris by a thin film which  could
withstand  gentle brushing with a soft brush.  However, due to the  require-
ment for a  very thin  film, so as not to cause appreciable beam attenuation,  v
problems arose as to choice of film and method of application.  Altogether,
four different  methods of application were tried utilizing (l) colloidion
which  is a film former,  (2) polyvinylpyrrolidone (PVP) a  film former, and
(3)  the combination of collodion and PVP.  Comments on the use of  these
materials  are  as follows:

      (1)   Collodion mixed directly into the filtrate for  simultaneous
           deposition sometimes tended to clog the filter.

      (2)   Collodion of PVP dropped gently onto the  substrate after
           filtration destroyed the uniformity by forming  spots.

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r- 	
1
I
1
\
I
\
\
\
\
i
I
l
1
1
1
1
1
1




^~-~~-



                                           FILTERING FUNNEL
                                           20 MM OPENING

                                           ^7 MM NUCLEPORE FILTER

                                           20 MM STAINLESS STEEL SCREEN



                                           FILTER HOLDER
                                          TAP FOR 3/8-IN. TUBE TO SUCTION
Figure 1.   Filter apparatus for 20 mm and 35 mm deposits prepared during
           Phase 1

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                             - 3 3/4"-
                             •3" 	
3"

                                1 1/2"
                                  \<
                           (^	35 mm—^|
                          	  2 1/4°—
Figure 2.   Filter apparatus for 35 mm deposits prepared during Phase 2
                                  10

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          An approximate calculation also showed the film to be
          too thick.

     (3)   Collodion sprayed on the substrate only after filtration
          was an improvement but did not provide adequate protection.

     (b)   The technique eventually selected involves a collodion spray
          before and after the deposition and was described in
          Section 3-

     The theory behind this technique is that the first spraying with 5%
collodion solution deposits a thin film of collodion on the filter onto which
the particles will rest.  The 0.]% collodion spray serves primarily to wet
the particles and bind them to the heavier collodion layer below the
particles.

     The concentration of the first collodion spray is not crucial but it
cannot be so great as to clog the filter.  On the other hand the second spray
concentration is important since it must meet the criterion of minimum x-ray
attenuation.  Experimentation and rough calculation showed that our use of
a 0.1% collodion solution could not deposit more than a few hundreths of a
micron thick film.  Since a thickness of 0.1 ym was acceptable, the film
thickness deposited was well within tolerable limits.

Mass Concentration
     Mass concentration of fines  was initially obtained using a Cahn Electro-
balance.  The only problem has been  the charging of the Nuclepore filters
which makes accurate weighing difficult.  This persistent problem with
microbalance measurements was partially overcome using a Polonium-210
radiation source  to discharge the membrane.  However, this has not been
entirely successful and great care is required to make a good measurement.
The final procedure adopted  is to filter a large volume of suspension and
weigh the residue on a semi-micro balance  (+0.05 mg sensitivity).  See
Section 5 for further details.

Mounting the Completed Filter

     During the process of mounting  the filters, there  is a tendency for
them to wrinkle.  This occurs because the upper retaining clip exerts
stresses on the filter and bottom holder which don't exist before the clip
is inserted.  Apparently this difficulty cannot be surmounted with the
filter holders presently in  use.

Packaging the Completed Filter

     There  is no  problem associated  with packaging the  standards.
                                      11

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                                 SECTION 5

                            COMPLETED STANDARDS


     A total  of 2k standards have been prepared.   These compounds are
Cr203, Mn02,  RhO,, Fe20_,  V205,  Sb203, CaF2,  AKO.,  As20o,  Ti02,  CuO, NiO,
BaSO/p Co Olj, PtO, ZnO,  PdO, Sn02,  Ag, Si02>  Mo03? HgO, W03 and NaCl.  The
calibration of these standards is purely gravimetric as described in the
next section.

CHARACTERIZATION OF COMPLETED STANDARDS

Mass

     The correlation between deposited mass  and volume of the suspension is
first determined by filtering and weighing a  large (approximately 1000 ml)
volume and weighing the  resulting deposit. This  correlation is used for both
size standards since the same stock suspension is used throughout.   Table 1
gives the gross and tare weights determined  from multiple measurements of the
calibration deposit.

     The volume of stock solution used for the heaviest deposit is  tabulated
in Table 2 along with the resulting compound  and elemental  mass per unit
area.  The larger deposition diameter is 35  mm.  The surface density for the
20 mm deposit can be obtained from the 35 mm deposit with allowance for the
reduced area and the fact that only half as  much solution is used for the 20
mm deposits.

     Based on the deposits in Table 2, the reported mass values for the
filters of each size are given in Table 3-

     The largest source  of error is introduced by the dilutions and this
depends on the size of the graduate used and  the number of dilutions.  The
maximum error in a 35 mm deposit would be for Mn02 for which a 5 ml cylinder
was used for 3 measurements.  This introduces, in conjunction with 2
measurements  of 50 ml, an uncertainty of about 3.6% for the maximum dilution.
Dilution errors for other cases will  be less.

Chemical Purity

     Table 4  summarizes  the available data on the chemical  compounds used
for the standards.
                                     12

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Table 1.    CALIBRATION DATA  FOR  STOCK SUSPENSION

Compound
Group A
CaF2
A12°3
SiO,
AS2°3
TiO,
CuO
NiO
BaSO^
C°3°4
Pto2
ZnO
MoO.
Group B
PdO
Sn02
Ag
HgO
WO
NaCl
Cr2°3
Mn02
RhO,
Gross
Weight

.02211
.01756
.01524
.01438
.01600
.01478
.01598
.01868
.01485
.01835
.01675
.01723

.01694
.01622
.02442
. 01604
.01790
.01937
.05797
.03804
.05011
Tare
Weight

.01423
.01418
.01343
.01264
.01409
.01385
.01422
.01453
.01386
.01466
.01413
.01416

" .01421
.01421
.01386
.01428
.01464
-01394
.01362
.01378
.01401

Net Deposit, g

.00788
. 00338
.00181
.00174
.00191
.00093
.00176
. 0041 5
.00099
.00369
. 00262
.00307

.00273
.00201
.01056
-00176
.00326
.00543
.04434
.02426
.03610
Vo 1 ume
Filtered, ml

1000
1000
500
1000
1000
1000
1000
1000
500
1000
1000
1 GG-0

500
1000
1000
1000
1000
1000
500
500
400
                         13

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Table 1  (cont.)

Compound
Fe2°3
V2°5
Sb2o3
Gross
Weight
.02485
.01778
.02333
Tare
Weight
.01515
.01508
.01400

Net Deposit, g
.00970
.00270
.00933
Vo 1 ume
Fi 1 tered,
100
500
500

ml




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Table 2.   SURFACE DENSITY CALCULATION FOR HEAVIEST 35 MM STANDARD

Compound
Group A
CaF2

A1203
Si02
As203
Ti02
CuO
NiO
BaSOit
Co30/,
Pt02
ZnO
Mo03
Group B
PdO
Sn02
Ag
HgO
wo3
NaCl

Cr203
Mn02
Suspension
Concentration,
mg/ml

0.00788

0.00338
0.00362
0.00174
0.00191
0.00093
0.00176
0.00415
0.00198
0.00369
0.00262
0.00307

0.00546
0.00201
0.01056
0.00176
0.00326
0.00543

0.08868
0.04851
Volume Used for Mass Delivered to
Heaviest 35 mm
Deposit, (ml)

500

500
500
500
500
500
500
500
500
500
500
500

500
500
500
500
500
500

10
5
Heaviest 35 mm
Depos i t , ug

3,940

1,690
1,810
870
955
465
880
2,075
990
1,845
1,310
1,535

2,730
1,005
5,280
880
1,630
2,715

886.8
242.6
Compound
Densi ty

409

176
188.1
90.4
99.3
48.3
91.5
215-7
102.9
191.8
136
159.6

283.8
104
549
91-5
. 169
282

92.2
25-2

Gravimetric
Factor

0.513 
-------
Table 2 (cont.)

Suspension
Volume Used for
Concentration, Heaviest 35 mm
Compound
RhO,
Fe263
V205
Sb»0«
mg/ml
0.09024
0.09698
0.00540
0.01866
Deposit, (ml)
10
10
10
20
Mass Delivered to Compound
Heaviest 35 mm
Depos it, yg
902.4
969.8
54.0
373.2
Density
yg/cm2
93.8
100.8
5.61
38.8
Gravimetric
Factor
0.763
0.699
0.555
0.835
Elemental
Density,
yg/cm2
71 .6
70.5
3.12
32.4

-------
Table 3.   ELEMENTAL DEPOSITS ON THE SUPPLIED AEROSOL  STANDARDS
Compound
CaF2(Ca)
(F)
Al.O
^m ^
sio2
AS2°3
Ti02
CuO
NiO
Ba SO^Ba)
Co 0,
j *i
Pto2
ZnO
MoO-
Group B
PdO
Sn02
Ag
HgO
WO-
NaCl (Na)
(Cl)
Elemental
210 ,
199 ,
92.9,
87-9,
68.5,
59.6,
38.6,
71-9,
127 ,
75.5,
165 ,
109 ,
106 ,

247 ,
82.3,
549 ,
84.7,
134 ,
111 ,
171 ,
Mass on the 35 rnm
105
99
46
43
34
29
19
35
63
37
82
54
53

123
41
274
42
67
55
85
9
.5,
.5,
-9,
.2,
.8,
.3,
.9,
.4,
• 8,
.4,
.7,
.2,

,
.2,
,
• 3,
.2,
-5,
.6,
21
19
9
8
6
5
3
7
12
7
16
10
10

24
8
54
8
13
11
17
.0 ,
.9 ,
.29,
.79,
.85,
-96,
.86 ,
.19,
.7 ,
.55,
.5 ,
• 9 ,
.6 ,

-7 ,
.23,
.9 ,
.47,
.4 ,
.1 ,
.1 ,
10.
9.
4.
4.
3.
2.
1.
3.
6.
3.
8.
5.
5.

12.
4.
27.
4.
6.
5.
8.
Depos i ts ,
5 ,
95,
65,
39,
42,
98,
93,
59,
34,
78,
24,
47,
32,

3 ,
12,
4 ,1
23,
72,
55,
56,
4
3
1
1
1
1
0
1
2
1
3
2
2

4
1
1
1
2
2
3
.20 ,
.98 ,
.86 ,
• 76 ,
• 37 ,
.19 ,
.772,
-44 ,
.54 ,
.51 ,
.29 ,
• 19 ,
.13 ,

.94 ,
.65 ,
,0 ,
.69 ,
.69 ,
.22 ,
.43 ,
. 2
yg/cm
2
1
0
0
0
0
0
0
1
0
1
1
1

2
0
5
0
1
1
1
.10
• 99
.929
.879
.685
.596
.386
.719
.27
.755
.65
.09
.06

.47
.823
.49
.847
.34
.11
.71
                               17

-------
                           Table 3 (Cont'd)
              Elemental Mass Density  „       Elemental Mass Density „
Compound   on the 35 mm Deposits,  g/cm    on the 20 mm Deposits, g/cm
Cr203          63.1  ,   6.31 ,  0.631,           96.6 ,   9-66 ,  0.966
Mn02           15.9 ,   1.59 ,  0.159,           24.4 ,   2.44 ,  0:244
Rh02           71.6 ,   7-16 ,  0.716,          109-6 ,  10.96 ,  1.096
Fe203          70.5 ,   7-05 ,  0.705,          107-9 ,  10.79 ,  1.079
V2°5            3.12,   0.312,  0.312,            4.77,   0.47.7,  0.0477
Sb0           32.4  ,   3.24 ,  0.324,           49-6 ,   4.96 ,   .496
                                  18

-------
Table 4.   ANALYSIS DATA .FOR COMPOUNDS USED IN DELIVERED STANDARDS
Compound Supplier
CaF. Spex
Al-0, Johnson-Matthay (JMC) 345
^ J




SiO, JMC 425
£i


As00, JMC 642
2 3



Ti00 JMC 435
2

CuO JMC 40







NiO JMC 895







BaSO. Spex




Lot No. Analysis Data
99.999S5
S.52652E Si 3 Ppm
Ca 2
Fe 2
Mg 1
Cr <1
Cu <1
S. 53797 Ca 3 Ppm
Fe 2
Mg <1
Ag <1
S.52136C Sb 6
Si 2
Fe 1
Cu <1
Mg <1
S. 52138B Si 5
Na 2
Mg <1
S.52378A Bi 6
Si 1
B <1
Cd <1
Ca <1
Fe <1
Mg <1
Ag <1
S.53375/b Al 3
Bi 3
Si 3
Ca <1
Cr <1
Cu <1
Fe <1
Mg <1
10741 Ba 57-5%
Mg 1-5 ppm
Si 1-5
Ca 0.5-2
Na 0.5-2
Fe 0.5-2
                                  19

-------
Table 4 (cont.)
Compound Supplier
Co.O. JMC 875
3 4






PtO- Research Organic/Inorganic
ZnO JMC 155







MoO. JMC




PdO Spex







SnO JMC




HgO JMC 191





Lot No. Analysis Data
S. 5095 ID 1 Si 7 ppm
Ni 5
Fe 2
Al <1
Ca <1
Cu <1
Mg <1
Ag <1
99-9%
S.52971B Fe 3 ppm
Cd 2
Mg 2
Si 1
Ca <1
Cu <1
Mn <1
Na <1
S. 52534 Mg 5 ppm
Cu 3
Fe 3
Ca <1
Si <1
11741 Fe 5-20 ppm
Sn 5-20
pd 87-9%
pt 10-30 ppm
Si 10-30
Bi 5-20
Pb 5-20
Mg 5-20
S53523C C Si 10 ppm
Ca 3
Al 1
Mg <1
Na <1
S 53625 Si 7
Cr 2
Cd 1
Cu <1
Mg <1
Ag <1
       20

-------
Table k (cont.)
Compound Suppl ier
Ag Aremco Prod.
WO. JMC
Sb20 Spex
Cr20 JMC 705
Fe20- Pfizer
Mn02 JMC 815
Rh02 Spex
Lot No.
Analysis Data
99-9%
S.5M34D Mo 1
Mg <1
077^1 Sb 82.2%
Si 2-5 ppm
Mg 0.5-1 ppm
Cu 0.5-1
Fe 0.5-1
Bi 0.5-1
S.51597F Si 4 ppm
Na 3
Cu <1
Mg <1
R3098R ' Fe20 99-5%
Lot 55 Si 62 200 ppm
CaO 110
MgO 50
Na 50
In 70
Mn 660
Pb 12
As <1
Hg <0.1
Water soluble
salts 0.0k%
S.51800A Si 5 ppm
Mg 2
Ca 1
Cu 1
10691 Ni 50-100 ppm
Pt 5-20
Pd 5-20
Fe 1-5
Mg 1-5
Cu 1-5
Pb 1-5
Ru 5-20
       21

-------
                             Table k (cont.)
Compound
V2°5
         Supplier
JMC
  Lot No.
S53459B
Al
Ca
Cu
Fe
Mg
Si
Analysis Data

1  ppm
                                   22

-------
Size and Uniformity

     Visual  inspection of  the  completed  standards  by optical microscopy
indicated most particles were  1 micron or  under  in size.  An occasional  larger
particle, probably an agglomeration  of smaller particles, up to  10 micron
can be seen.  The particle distribution  presents a visually uniform
appearance.

      Initially,  an analysis of size  and  uniformity was  planned using  an
electronic  image analyzer.  The confused image created  by the numerous
pores  in  the Nuclepore  filter, however,  has  prevented using this quick but
quantitative technique.   Data  from visual  microscopy are given  in Table  5.

X-Ray  Characterization

      The  x-ray  absorption  characteristics  for all  the compounds  of  interest  ,
are given in Table  6.   This includes the absorption coefficient  and  the  mean
free path for the fluorescent x-rays  in  the specified compound.   Most  of  the
data are  for the K  lines  but data for L  and  M lines is  included  if  these
are likely to be used.

Particle  Size Effects

      In thin deposits such as these  standards, allowance for the effect  of
 particle  size on response depends on the x-ray absorption characteristics,
 i.e., self-shielding properties,  of  the  particles. For a complete  des-
 cription  of the effect,  the energy distribution  of both the  incident  and
 emitted x-rays as well  as the typical geometry of  the particle must  be well
 specified.  In practice,  the particle geometry  is  often poorly or only
 approximately determined.

      If information on the approximate particle  size  is available, one can
 estimate the expected effects using  formulas such  as  reviewed  in
 Reference  (2).

      Alternatively, one can determine the effective particle size by making
 two separate fluorescence measurements on each  sample  using  two  different
 energies of excitation radiation  (3).  Since the sample mass  is  the  same
 in both cases,  any observed difference in response can  generally be
 ascribed to particle size effects.  Corrections  for the effect  can  then  be
 made.

      A first order estimate of the significance  of particle  size or  self
 shielding effects can be made by comparing the particle diameter with the
 radiation mean free path.  A  large ratio of diameter to mean  free path
 obviously  implies significant self absorption within  the  sample.

      The approximate losses can be put on a quantitative basis by calculating
 the fraction of x-rays escaping from a spherical  particle,  (radius  = R),
 with x-rays generated uniformly throughout the volume.   This  assumes
                                       23

-------
Table 5.    PARTICLE  SIZE  DATA  FOR  LIGHT ELEMENTS

Compound
CaF,
2



BaSO,
T



MoO,
J



NaCl





Al 0
*- J



Size Range
<2 ym
2-5
5-10
>10

<2 ym
2-5
5-10
>10

<2 ym
2-5
5-10
>10

<5-3 ym
5.3-15-9
15.9-26.5
26.5-53
>53

<5.3 ym
5.3-10.6
10.6-21.2
>21.2
Number
of Particles
185
293
39
3
520
37
291
69
3
400
177
227
10
2
416
71
146
135
120
31
503
238
156
33
6
Number
Percent
35. 6£
56.3
7-5
0.6
100.0%
9.22
72.8
17.2
0.8
100.0%
42.5%
54.6
2.4
.5
100.0%
14.1%
29.0
26.8
23.9
6.2
100.0%
55.0%
36.0
7-6
1.4
                           433           100.0%
                       24

-------
                                     Table 6

          X-RAY CHARACTERISTICS  FOR ELEMENTS AND COMPOUNDS OF  INTEREST
(E = X-Ray Energy,  p - Mass Absorption  Coefficient, and A = X-Ray Mean Free  Path)

Element
Al

Sb
As
Ba

Br


Ca

Cd
Cl
Cr


Co

Cu

Au
F
Fe

Pb


Mg


Mn


Compound
Al
A12°3
Sb2°3
AS2Q3
BaC03
BaSO,
4
PbBr2
NaBr
RbBr
CaCO
Ca(OH)2
CdO
LiCl
Cr
Cr 0
2 3
Co
C°3°4
Cu
CuO
Au2°3
LiF
Fe
Fe2°3
Pb
Pb02
Pb3°4
Mg
MgF
MgO
Mn02
MnCO_
Density
gin/cm-^
2.702
3.965
5.2
3.738
4.43
4.50
6.66
3.203
3.35
2.710
2.24
6.95
2.068
7.20
5.21

8.9
6.07
8.92
6.4
it
2.635
7.86
5.24
11.34
9.375
9.1
1.74
*
3.58
5.026
3.125



Line
Al
Al

As


Br
Br
Br
Ca
Ca

Cl
Cr
Cr

Co
Co
Cu
Cu

Ka
Ka
-
Ka
-
-
Ka
Ka
Ka
Ka
Ka
-
Ka
Ka
Ka

Ka
Ka
Ka
Ka
-
F Ka
Fe
Fe



Mg
Mg
Mg
Mn
Mn
Ka
Ka
-


Ka
. Ka
Ka
Ka
Ka
1
1

10


11
11
11
3
3

2
5
5

6
6
8
8

0
6
6



1
1
1
5
5
E
keV
.487
.487

.53


.91
.91
.91
.691
.691

.622
.412
.412

.926
.926
.040
.040

.679
.401
.401



.254
.254
.254
.895
.895
U
X
cm /gm
385.
912.

30.


62.
27.
35.
.116.
125.

175.
88.
72.

64.
52.
53.
45.

832.
7
1

1


0
2
0
4
4

1
2
8

9
8
7
5

2
71.4
57.



463.
2051.
1245.
61.
52.
3



6
1
4
6
1
Micron
9.
2.

88.


24.
114.
85.
31.
35.

27.
15.
26.

17.
31.
20.
34.

4.
17.
33.



12.
*
2.
32.
60
77

9


2
8.
3
7
6

6
7
4

3
2
9
3

56
8
3



4

24
3
E 2V X E 2y X
Line keV cm /gm Micron Line keV cm /gm Micron
-
-
Sb Lai 3.605 365.6 5.26
As Lai 1.282 1737.6 1.54
Ba Lai 4.465 239.5 9.43
Ba Lai 4.465 265.3 8.38
Br Lai 1.480 2103.6 0.714
Br Lai 1.480 1759.4 1.77
Br Lai 1.480 1413.8 .2.11
-
-
Cd Lai 3.133 453.0 3.18
-
.-
_

-
-
-
-
Au Lai 9.712 114'.4 * Au Ma 2.12 1068.1 *
-
-
-
Pb Lai 10.55 116.6 7.56 Pb Ka 2.342 983.0 .897
Pb Lai. 10.55 98.5 10.83 Pb Ma 2.342 890.0 1.20
Pb Lai 10.55 106.3 10.3 Pb Ma 2.342 930.2 1.18
-
-
-
-
61.4

-------
                                                                    Table 6  (cont.)
M
Density
Element Compound era/cm3
Hg
Mo


Ni

Pd
P
Pt
K

Rh
• Se
Si
Ag

Na

S

Sn

Ti
W

U'
V
Zn

_ _, -f- T 	 „ 	
HgO
Mo
Mo03
MoS2
Ni
NiO
PdO
P3N5
Pto2
KHC03
KN03
Rh02
Se02
Si02
Ag
Ag20
Na2SO,
Na2C03
S
(NH ) SO
Sn
SnO.
Ti02
W
wo3
U3°8
V2°5
Zn
ZnO
_^__ »~t • 	 i —
11.1
10.2
4.692
4.80
8.90
6.67
8.70
*
10.2
2.17
2.109
*
3.95
2.65
10.5
7.143
2.68
2.532
1.92
1.769
ft
7.28
6.95
4.26
19.35
7.16
8.3
3.357
7.14
5.606
Line
_
Mo Ka
Mo Ka
Mo Ka
Ni Ka
Ni Ka
-
P Ka
-
K Ka
K Ka
-
Se Ka
Si Ka
-
-
Na Ka
Na Ka
S Ka
S Ka
-
-
Ti Ka
-
-
-
V Ka
Zn Ka
Zn Ka
E
keV

17.46
17.46
17.46
7.473
7.473

2.014

3.313
3.313

11.21
1.740


1.041
1.041
2.307
2.307


4.512



4.951
8.631
8.631
U
2

19.1
13.2
15.65
58.9
49.7

322.2

143.9
148.5

26.2
667.8


2565.8
2349.6
239.4
323.6


92.6



77.3
49.0
41.4
X
Micron

51.
161.
133.
19.
30.

*

32.
31.

96.
5.


1.
1.
21.
17.


25.



38.
28.
43.

3
.5
1
1
2



0
9

6
65


45
68
8
5


4



5
6
1
Line
Hg Lai
Mo Lai
Mo Lai
Mo Lai
-
-
Pd Lai
-
Pt Lai
-
-
Rh Lai
Se Lai
-
Ag Lai
Ag Lai
-
-
-
-
Sn Lai
Sn Lai
-
W Lai
W Lai
U Lai
-
-
.
E
keV
9.987
2.293
2.293
2.293


2.838

9.441


2.696
1.379

2.984
2.984




3.443
3.443

8.396
8.396
13.612



2 X
cm /gm Micron
115.1 7.83
728 1.35
633.1 3.37
533.7 3.90


514.9 2.23

114.4 8.57


519.1 *
1543.4 1.64

521.9 1.82
500.4 2.80




437.4 3.14
374.5 3.84

150.8 3.43
121,9 11.5
80.7 14.9



E „ X
Line keV cm /gm Micron
Hg Ma 2.195 1043.2
-
-
-
-
-
-
-
Pt Ma 2.048 1109.6 0.884
-
-
-
-
-
-
-
-
-
-
-
-
-
-
W Ma 1.774 1465.6 0.353
W Ma 1.774 1351.0 1.03
U Ma 3.165 615.0 1.96
-
-


-------
negligible attenuation of  the  stimulating  radiation  within  individual
particles.  The fraction,  f, of  x-rays which  escape  in  any  given  direction
is given by (see Appendix):


     f	^     _J_^  (l-e~2ER)(l+ER)
          (ER)Z   2(ER)3


where E  is the macroscopic x-ray absorption cross  section  (=  1/mean-free-
path).   Figure 3  is  a  plot of  f  versus the size  parameter D/A,  where
D= diameter and X  =  mean  free  path.

     An  alternative  way  to plot  the  same equation  is given  in  Figure k.
Explicit values of the mean  free path, A,  such as  given for the calibration
standards, can be  used to enter  the  graph.  The  emission rate  for different
size  spherical particles  can then be read  out directly.

      Generally,  if the emission  is to be greater than 90% for  1 micron
particles, then  the  mean  free  path should  be  greater than 4-7  micron.

      The tabulated values for  the mean free path In  the standards provided
 indicate that they are all greater than  5  micron except for AKO  ,  PbO, SnCL,
Ag and  MoO^.   Losses should  therefore be less than 10%  for  the  deposits
since they are generally under 1 micron  in size.

MATERIALS SELECTION  AND  TARGET VALUES

      Catalogs from about 100 chemical  supply  houses  were examined to
determine sources  of material.  The  initial list of  possible compounds was
 based on chemical  simplicity,  purity available,  particle size,  certification,
etc.   A second list  of simple  compounds  which are  likely to be  present in
aerosol  samples  was  also prepared as a useful alternative compound.  The
 initial  screening  of these lists produced  a list which  contained  generally
acceptable  compounds.  Table 7 contains  the list of  chemical forms  finally
 selected for  deposition.

      The formula  weights, gravimetric factors, and desired  surface  density
for  each of  these  compounds  is given in  Tables 8 and 9.
 ^Density not available.
                                      27

-------
ho
oo
          0.8  -
          0.6 -
      o
      o
V
Q.
      UJ
                                                                1   I  I  I  1  1 I 1 I
          0.4 -
          0.2 -
           0.01
                      Figure 3-
                                      Diameter/ Mean-Free-Path    D/X

                           Probability of escape  from a uniform spherical source as a
                           function  of diameter/mean-free path
                                                                                                100

-------
WO
             §
             u
            2E
            £
            o
            5
               0.01
                  Q!
10
                                                Mean Free Path X (Micron)
100
1000
                               Figure ^».  Probability of escape from a uniform spherical  source
                                         as a function of diameter and mean-free-path

-------
Table 7.   CHEMICAL FORMS SELECTED FOR USE
         AS DEPOSITED PARTICULATES
Element
F
Na
Mg
Al
Si
P
S
Cl
K
Ca
Ti
V
Cr
Mn
Fe
Co
Ni
Cu
Forms
LiF
Na2CO
MgO, Mg, MgF2
A120 , Al
SiO,
P3N5
S, (NHit)2SOil
LiCl
KHCO-, KNO_
CaCO , Ca(OH)2
. Ti02
V2°5
Cr, Cr20_ .
MnCO,, Mn02
Pp Ppa A
1 C J 1 CM V A
Co, Co^
Ni, NiO
Cu, CuO
E1emen t
  Zn
  As
  Se
  Br
  Mo
 .Rh
  Pd
  Ag
  Cd
  Sn
  Sb
  Ba
  W
  Pt
  Au
  Hg
  Pb
  U
                                       Forms
                                 Zn,  ZnO
                                 Se02
                                 PbB>2>  NaBr,  RbBr
                                 Mo,  MoO
                                 Rh02
                                 PdO
                                 Ag20
                                 CdO
                                 Sn,  SnO
                                 Sb2o3
                                 BaCO.
                                 W, WO.
                                 PtCL
                                 HgO
                                 Pb,  PbO,
                                 U3°8
3
                     30

-------
                                   Table 8
ARF FIITFK
          DESIRED  DENSITY AND OTHER DATA FOR COMPOUNDS OF INTEREST
Fl ! HINT
SO UG 1 IT
AL

SI-
AS
R'

BR


Cfl

CD
Cl
CR
CR
CO

cu

AH
F
Ft

PS


HC


MN

HC
MO


Ml

PD
p
pr
K

RM
sr.
ii
(. ,

•i£




-,-••

r ,



V
IH
;N
FORHLILA
AL
AL203
SII203
AS203
B1C03
BAS04
PBBR2
NABR
RBB»
C«C03
CA(OH)2
cno
LICI
CR
CR203
CO
C0304
CU
cuo
AU203
LIF
F,
FE203
PI'
PB02
pp.3.04
Mi.
MCF7
MGO
MN02
MNC03
Ht.O
MO
M001
MOS2
Ml
NIO
P! 0
P SN5
PIO'
KHC03
KN01
Rl'02
sr o>
5IO,>
Al.
AG.-'O
N''.' S04
N/-7COJ
S
(Mil , >,'S04
N/1-/ Si)-,
•>:i
srm.
1 1 0 '
J
w.
•'
v>0',
'.U
/NO
FORMULA
WEIGHT
26.982
101.960
291.500
197.840
197.390
233.400
367.010
102.900
165.380
100.1190
74.094
128.400
42.392
51.996
15 1 .990
58.933
240.800
63.540
79.539
441.930
25.937
55.847
159.690
207.190
239.190
685.570
24.312
62.308
40.311
86.936
114.950
216.590
.95*1)40
143.940
160.070
58.710
74. /09
122.400
162.960
227.090
100.120
101*110
134.900
110.960
60.084
107.8/0
231.740
142.042
105.990
32.064
132.140
147.04?
1 1 R.690
! ^0»6">0
79.1: (•
'Ml. ',!l
! .
•i • .
im. no
65.370
81.169
ELEMENT
WEIGHT
26.982
93.464
243.900
149.840
137.340
137.340
199.820
79.909
79.904
40.080
40.080
112.400
39.493
51.996
103.990
58.933
176.799
63.940
63.540
393.<<30
18.998
55.847
111.640
207.190
207.190
621.570
24.312
24.312
24.312
54.938
94.938
200.590
95.')40
95., 140
95.940
58.710
58.710
106.400
92.0J2
195.090
39.102
39.102
102.905
78.960
28.0116
107.810
215.740
45.982
45.982
32.064
32.064
1,? .'164
i i n • i , 9 1
' 1 H . 1 <> 1
',7. "01

I s

- 1-00.1,80
6.5.370
65.170
USING .90 OF VOL. COMPOUND MASS
GRAVIMETRIC RESULTING ELEMENT (UG) ON SMALL
FACTOR
1.000
0.924
0.835
0.797
0.646
0.588
0.435
0.777
0.413
0.400
0.941
0.875
0.836
1.000
0.684
1.000
0.734
1.000
0.749
0.891
0.732
1.000
0.699
1.000
0.866
0.907
l.OOO
0.390
0.603
0.632
0.478
0.926
1.000
0.667
0.599
1.000
0.786
0.869-
0.570
0.859
0.391
0.387
0.763
0.712
0.467
1.000
0.931
0.324
0.434
1.000
0.2'.!
0.?26
1 .0 0
.76 «
.6 0
.0 :•!
..'•'!
'' "''.
.555
1 .0' U
• a i
OBNIITY
0.04W
0.0499
0.7656
4.9«3*
O.T696
0.76*6
0.7*16
0.7656
0.7616
0.7656
0.7656
0.0766
O.T696
0.7696
0.7656
0.7696
0.7696
0.7616
0.76)6
0.7656
0.7656
0.7656
0.7696
0.4994
0.4594
0.4594
0.7696
0.7656
0.7696
0.4944
0.4994
0.7696
0.7696
0.7656
0.7656
0.7656
0.7656
0.7656
0.1531
0.7656
O.0459
0.0459
0.7696
0.7656
0.153)
0.7656
0.7656
1.5312
1.5312
0.1531
0.1531
0.153
1. 76r>6
0.7656
••76",6
-. 7//',/
1 . . 6-i<
'•"' '''-
0.7.656
0.7656
0.7656
ttw/iacH) « i uor*i«e*
0.45*
0.454
7.656
41.937
7.616
7.616
7.656
7.656
7.696
7.696
7.656
0.766
7.696
7.656
7.656
7.696
7.696
7.696
7.696
7.696
7.656
7.656
7.656
4.944
4.594
4.594
7.656
7.656
7.656
4.594
4.594
7.656
7.656
7.656
7.656
7.656
7.656
7.656
1.531
7.656
0.454
0.459
7.656
7.656
1.531
7.656
7.656
15.312
15.312
1.531
1.531
1 .531
7. ,.',6
/..Ah
' . ' '^ 6
~ . • >, f
'. •>(,
• '•'•
7.656
7tf>56
7.^.56

Sit*
76.16
153.13
76.lt :
76.96
76.96 <
74.96
76.96
76.56
•76.96
7.66
76.16
76.56
76.56
76.96
76.96
76.16
76.96
76.56
76.56
76.56
76.56.
45.94
49.94
45.94
76.56
76.56
76.56
45.44
45.44
76.56
76.56
76.56
76'.56
76.56
76.56
76.56 '
15.31
76.56
4.99
4.59
76.96
.416
.761
.148
• II*
.334
.214
.Ml
.102
.84!
.BOB
.589
.716
.142
.142
.142
.27.4
.142
.433
.524
.2*4
.142
.4*2
.142
.627
.465
.142
.051
.209
.971
.573
.392
.142
.713
• 242
.142
.448
.614
.510
.657
.044
.124
.118
76. 56 4.415
15.31 6.721
76.56 3.142
76.56 3.375
1S3.13 4.705
153.1! 7.241
15.31 3.142
15.31 12.947
t'i.31 11.917
V6.1 3.142 .
76. '' i.98<)
76. *> >.240
V,.,1; . 3. 14.'
. .'- l.af ••
' .-. ' 3.7-)',
76.5 5.664
76.5. 3.142
76.5 3.910
TOTAL COMPOUND MASS
(UOI WITHIN SMALL AREA
AT RESULTING DENSITY
0.144
0.273
2.879
19.019
3.496
4.088
5.123
3.047
.4.478
6.007
4.447
0.279
2.876
2.409
3.516
2.409
3.276
2.409
3.011
2.698
3.284
2.405
3.439
1.443
1.666
1.592
2.405
6.164
3.988
2.284
3.020
2.997
2.405
3.609
4.013
2.405
3.061
2.767
0.844
2.800
0.370
0.373
3.153
3.380
1.029
2.405
2.584
14.860
11.089
0.481
1.983
2 . U 1
.•-.',,15
' .o-,4
., .01. >
. • )r>
', . <
.i ^ 1,
.',.337
2.405
2.994
1.44
2.73
28.74
140.91
34.96
40.68
55.23
30.47
49.76
60.07
44.47
2.79
28.76
24.09
39.16
24.05
32.76
24.05
30.11
26.98
32.84
24.05
34.39
14.43
16.66
15.92
24.05
61.64
39.88
22.84
30.20
25.97
24.05
36.09
40.13
24.09
30.61
27.67
8.44
28.00
3.70
3.73
31.93
33.80
10.29
24.05
25.84
148.60
110.89
',.81
19.83
2' .3
2'. .05

4 • . 1 .'
,-, , 1^
t •
> . w
4', .37
24.05

14.4
27.3
287.9
635.2
349.6
408. 6
552.3
109.7
447.8
600.7
444.7
27.5
287.6
240.5
351.6
240.5
327.6
240.5
301.1
269.8
328.4
240.5
343*9
144.3
166.6
159.2
240.5
616.4
398.8
228.4
302.0
259.7
240.5
360.9
401.3
240.5
306.1
276.7
84.4
280.0
37.0
37.1
315.3
338.0
102.9
240.5
25f).4
1486.0
1100.9

19IS.3
211.
?', -. ',
M-..4
4.1-.,'
•-. ., . .
.
' ••
4.3 1 . 7
240.5
299.4
THICKEST
COMPOUND
(UG/5QCM)
4.14
9.611
91.65
202.18
110.02
130.11
171.82
98.59
158.45
191.20
141.54
8.75
91.55
76.56
111.90
76.56
104.28
76.56
95.84
85.1-9
104.53
76.^1
109.47
45.94
53.03
50.67
76.56
196.22
126.95
72.69
96*12
82*67
76.5..
114.87
127.74
76.56
97.43
88*0"
26*85
119* 12
1 1 .76
11.88
100.37
107.59
32-76
76.5-
82*24
473 .02
352.96
15.11
63.10
67 -H '.
7 6 « '

' ' 7 • " 1
i

1 .
1 >8 . 04
76 . ','


-------
           L/ KG!  ARC  I II TEI
                                                   Table  9
                     E7~  DESIRED  DENSITY AND OTHER DATA  FOR COMPOUNDS OF  INTEREST
ro
Fl I Ml NT
SOUGHT FORMULA
Al

SI
AS
B'

BR


C.«

CD
CL
CR
C»
CO

CU

flu
r
fp

pp


M(


MM

Hf.
MO


Nl

PP
P
pr
K

RM
sr
SI
AC

NA

',


SN

TI
•4

II
V
ill
ZN
Al
Al 203
S. 201
A',203
BAC03
BAS04
PBBR2
NABR
RBBR
C/iCOl
CA ( OH 1 2
ciio
LICL
ci;
CR203
CO
Q0304
en
cuo
A1I203
LIF
f>
FE203
PP
PP02
PP304
M(
MGP2
MGO
MM02
MNC03
nro
MO
M003
M052
Nl
NIO
fr.O
P3N5
PI02
KHC03
KNOT
RN02
Sr02
S 102
A'
AC20
N "•? S04
NA2C03
S
INH4 l.'SO'i
NJVSIJ4
SM
SNOJ
rici '
w
<*c
u »•
V:>0',
^M
•/MO
FORMULA
WEIGHT
26.982
101.960
291.500
197.840
197.390
233.400
367.010
102.900
165.380
100.090
74.094
128.400
42.392
51.996
151.990
58.033
240.800
63.540
79.539
441.030
25.937
55.847
159.600
207.190
239.190
685.570
24.312
62.308
, 40.311
86.936
114.050
216.590
95 .^)40
143.940
160.070
58.710
74 . , 09
122.400
162.960
227.090
100.120
101.1 10
134.900
110.960
60.084
107.8,0
231.740
142.042
105,990
32.064
132.140
142.042
1 18.6QO
1'50. h'lO
79.11. 8
. 1 !l3. '.1
' * 1 - ' •
- ' '• !"
iKl. ftj
65 - 1 70.
n 1.360
ELEMENT
WEIGHT
26.912
S3. 964
243.500
149.840
117.1140
137.340
159.820
74.909
79.909
40.080
40.080
112.400
35.453
51.996
103.990
58.933
176.Y99
63.540
63.540
393.') 30
18.098
55.847
111.690
207.190
207.190
621.570
24.312
24.312
24.312
54.038
54.938
200.590
05. )40
05.V40
95.1:40
58.710
S8.710
106.400
02. "22
195.090
39.102
39. .102
1O2.005
78.060
28.11116
107. RVO
215.740
45.982
45.082
32.064
12.064
32. '64
( Ifl.f.QO
118, f>0(l
47.QOO
( . r "
, 1, V
; ' ., • ij
100.il Hi
••1,5. 1711
65, 570
COMPOUND MA 8 8
GRAVIMETRIC DEI I RED tLEMINT 2f>
1 .O'"0
,78fl
,6 0
1 .0 0
.751
. M-lfl
.555
•l.O'.O
. S 1
O.O300 0.3OO
0.0)00 O.SOO
O.SOOO 9.000
3.0000 50.000
0.1000
0.1000
o.sooo
0.5000
o.sooo
o.sobo
o.sooo
0.0500
0.5000
o.sooo
o.sooo
o.sooo
o.sooo
o.sooo
o.sooo
o.sooo
0.9000
0.9000
o.sooo
0.3.000
0.3000
0.31)00
0.5 100
0.5000
.000
•000
.000
.000
.000
.000
.000
.500
.000
.000
.000
.000
.000
.000
.000
.000
,000
,000
.000
.000
.000
.000
.000
.000
O.SOOO 5.000
0.3000 3.00O
0.3000 3.0OO
O.SOOO 5.000
0.5000 5.000
O.SOOO 5.000
O.SOOO 5.000
0.5000 5.000
O.SOOO 5.000
0.5000 5.000
0.1 1)00 1.000
0.5000 5.000
0.0300 0.-300
0.0300 0.3 0
0.5000 5.000
0.5000 5.000
0.1 000 1.000
0.5000 5.000
0.5.00 5.000
1.0000 10.0 0
1.0000 10.0 0
o.i oo 1.010
O.I 00 l.'MO
0.1 00 I.O.JO
"1.5 00 5, ''10.
0.5 -00 5." n
1.5 00 5, 'HI
'-.r, m •>. ' ,-,
,).'- -P) <-,. MI
1,' '1 l • , :
D.'I.OO 5.0,0
1 •« 5.i 00 5 . l ' 10
0.5 >Qi) 5,,'iOO
3.00
3.00 '
90.00
100. on
SO. 00
so.oo
so.oo
90,00
so.oo
90.00
90.00
5.00
90.00
90.01)
so.oo
50.00
so.oo
90.00
90.00
90.00
90.0')
SO.OO
50.00
30.00
30.00
30.00
50.00
SO.OO
50.00
30.0(1
30.00
SO.OO
50.00
50.00
90.00
50.00
So.oo
sn.Oii
10.0 )
50.00
3.00
3.00
50.00
50,0.1
10. On
50.0
50 , 0'fl
100,'! )
100. '1 '
10.0
1 ,.!>
1 ,1)
5, '.il
5 •• ) :
5' -,'J
5 .C
r,
'•.-••
5 i.'n
.Sii.O
5'i. 0 t -
».*tl
11.178
11.S18
12.TO3
\ S«IMW
• • £ AA 4tfA
" : W~» V*
lit 094
12.384
19.912
24.026
17.7*6
1O4941
11.S04
9.621
14.062
9.621
13.104
' 9,621
12.044'
10.793
13.135
9.621
13.796
9,621
11.107
10.612
9.621
24.658
IS. 453
15.225
20.131
10.389
9.621
14.435
16.052
9.621
12.243
11.068
16.873
11.199
24.635
24.878
12.613
13.520
20.582
9.621
10.33S
29.720
22.177
9.621
39.650
42.6 1
9.621
i2.2l5
16.04(1
0.621
1 1- . 13.
1 1 . 14r.
17.346
9.621
11.976
rOTAL COMPOUND MASS
US) WITHIN LARGE AREA
IT OEIIRED OBNI1TY
0.214
0.549
5.759
31.110
S.413
.17*
11>047
6.195
9.9S6
12.013
8.143
O.SSO
5.752
4.811
7.031
4.811
6.552
4.811
6.022
5.397
6.9r>8
4.811
' 6.878
2.886
3.332
3.184
4.811
12.329
7.976
4.567
6.039
5.194
4.811
7.217
8.026
4.811
6.121
5.534
1..687
5.600
0.739
0.746
6.306
6.760
2.058
4.811
5.167
29.720
22.177
0.062
3.065
4,262
4,81 1
6.108
li,024
4.81 1
6.11 7
',.'.75
8.673
4.811
5.988
2*1*
5.4!
S7.99
381.10
69.1!
Si. 75
110.47
61.99
99.56
120.13
18.93
S.SO
S7.S2
48.11
70.31
48.11
65.52
48.11
6O.22
S3. 97
65.68
48.11
68.78
28.86
33.32
31.84
48.11
123.29
79.76
4S.67
60.39
91.94
48411
72.17
80.26
48.11
61.21
95.34
16.87
56.00
7.39
7.46
63.06
67.60
20.58
48.11
51.67
297.20
221.77
0.62
39.65
42.6 '
4li.ll
61.08
80.24
4li. 1 1
6 •• 7
5 ,7i
. 8I-.73
4n. 11
59,88
28.9
54.5
57S.9
1270.3
691. J
•17.9
1104*7
614. S
095.6
1201.3
889.3
55.0
S75iZ
481.1
703.1
481.1
695.2
481.1
602.2
539.7
696.8
481.1
687,0
288.6
333.2
318.4
481.1
1232*9
797.6
4S6.7
603.9
519.4
481.1
721.7
802.6
481.1
612.1
553.4
168.7
560.0
73.9
74«6
630.6
676.0
205.8
481.1
516.7
297. '.0
2217.7
96.2
30f»5
426.
481.1
610.8
803.4
481.1
60|,.7
H .5
867.3
481,1
59 n, a
THICKEST
COMPOUND
(UOVSQCM)
3.00
S.67
59.86
132.03
71. 8S
84.97
114.82
64.39
103.48
124.86
92.43
5.71
59.79
50.00
73.08
50.00
68.10
50.00
62.59
56.09
68*26
50. OU
71.49
30.00
34.63
33.09
50.00
128.14
82.90
47.47
62.77
53.09
50.00
75.02
83.42
•50.00
63-63
57.1,2
17-54
58.20
7.68
7.76
65-5S
70.26
21.30
50.0,
53.71
3 18.01
230.5
10.0
41-.'
44. 30
50.0
(> 3 . 4 8
83. '.0
60,0
f 3 ,••'
',«.-,.,
00-15
50.0
62,24

-------
                                 REFERENCES

1.   Wagman, J., R. L. Bennett and K. T. Knapp.  Simultaneous Multiwave
    Length Spectrometer for Rapid Elemental Analysis of Particulate
    Pollutants.  X-Ray Fluorescence Analysis of Environmental Samples,
    Ann Arbor Publishers,  Inc., Ann Arbor, Michigan, 1977-

2.   Berry, P. F. , T. Furuta and J. R.  Rhodes, Particle Size Effects in
    Radioisotope X-Ray Spectrometry, pp. 612-632  in Charles S. Barrett,
    J. B. Newkirk and Gavin R. Mallett, Eds., Advances in X-Ray Analysis,
    12 (1969).

3.   Giauque, R. G.,  L. Y.  Goda and R.  B. Barrett, X-Ray Induced X-Ray
    Fluorescence Analysis  of  Suspended Air Particulate Matter, LBL-2951
    (June  197*0.
                                       33

-------
       APPENDIX A




SELF ABSORPTION IN A SPHERE

-------
                                 APPENDIX A

                        SELF ABSORPTION  IN A SPHERE
    The fraction of radiation escaping from a uniform spherical source in a
specific direction can be obtained by direct  integration of the attenuation
equation.  Figure 5 shows a volume element, with an attenuation path 1, in
a sphere with radius R.  Using Z for the macroscopic absorption cross section,
the total radiation escaping from the disc element is for unit source
strength.
    Total out
    from disc
                 r=/R2-X2
27ire~Z1 dr
                  r=o
2ire
                       r=/R2-x2
  -Z/R2-r2  „
re         dr
                                         r=o
                                                 2   2
After changing  the  integration  variable  to y = /R -r  and  integrating by
parts, one gets
    Total out  m  2   £XL  R e"ER
    ,-     i.    —  £ lit  I   V c
    from di sc         j.   2-
                             -ZR
 Integrating  the  disc contribution over the entire sphere  and factoring the
 final  form gives
     Total  out   _
     from sphere
  X=R

  Total out
  from disc


  X=-R
                                       35

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Figure 5-   Geometry and notation used for describing escape of
           x-ra.v from within a spherical particle
                             36

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For small values of £, this can be simplified as follows:
    Total out for = k R3  (]
    small £         3


The fraction of  radiation  escaping  compared to a non absorbing sphere is
obtained by dividing either of  the  last  two equations by 4irR3/3.
                                       37

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  APPENDIX B




SERIAL NUMBER LISTS
       38

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           IIT RESEARCH INSTITUTE
            Element

Compound    Sought



COoO,       Co
	-J H-
            Ti
ZnO         Zn
NiO         Ni
 Pt02
Serial #
001-1
002-2
003-5
004-10-
005-50
006-100
007-1
008-2
009-5
010-10
011-50
012-100
013-1
014-2
015-5
016-10
017-50
018-100
019-1
020-2
021-5
022-10
023-50
024-100
031-1
032-2
033-5
034-10
035-50
036-100
Weight
(ug/cm )
0.755
1.51
3.78
7.55
37.8
75.5
0.595
1.19
2.98
5.95
29.8
59.5
1.09
2.18
5.45
10.9
54.5
109
0.718
1.44
3. .59
7.18
35.9
71.8
1.65
3.. 30
8.25
16.5
82.5
165
                        39

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           IIT RESEARCH INSTITUTE

            Element                 Weight
Compound    Sought     Serial #    (ug/cm2)

CaF0        Ca         037-1          2.10
	2                   038-2          4.20
                       039-5         10.5
                       040-10        21.0
                       041-50       105
                       042-100      210

CaF9        F          037-1          1,75
	L                   038-2          3.50
                       039-5          8.75
                       040-10        17.5
                       041-50        87.5
                       042-100      175

MoO-        Mo         055-1          1.07
— 3                   056-2          2.14
                       057-2          5.35
                       058-10        10.7
                       059-50        53.5
                       060-100      107

BaSO,       Ba         061-1          1.27
	 4                  062-2          2.54
                       063-5          6.35
                       '064-10  .      12.7
                       065-50        63.5
                       066-100      127

BaSO,       S         061-1            .296
    *                  062-2            .592
                       063-5          1.48
                       064-10         2.96
                       065-50        14.8
                       066-100       29.6

A100,       Al         067-1          1.08
   L J                  068-2          2.16
                       069-5          5.40
                       070-10        10.8
                       071-50        54.0
                       072-100      108.

PdO         Pd         073-1          2.47
                       074-2          4.94
                       075-5         12.4
                       076-10        24.7
                       077-50       124
                       078-100      247

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IITRI  X-RAY FLUORESCENCE STANDARDS
Compound
iL°9
£•




Cup





MaP





wo.
— 3




SnO.
— 2




As00_
—2 3




Element
Sought Serial #
Si 043-1
044-2
045-5
046-10
047-50
048-100
Cu 049-1
050-2
051-5
052-10
053-50
054-100
Hg 079-1
080-2
081-5
082-10
083-50
084-100
w 091-1
092-2
093-5
094-10
095-50
096-100
Sn 097-1
098-2
099-5
100-10
101-50
102-100
As 103-1
104-2
105-5
106-10
107-50
108-100
WeighJ
(ug(cm )
.879
1.76
4.40
8.79
44.0
87-9
.386
.772
1.93
3.86
19-3
38.6
.847
1.69
4.23
8.47
42.3
84.7
1.34
2.69
6.72
13.4
67-2
134
.832
1.66
4.16
8.32
41.6
83-2
.765
1.53
3.82
7.65
38.2
76.5
                   41

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Compound




  NaCl
  Na
Element
Sought
Na





Cl





Ag






Serial #
085-1
086-2
087-5
088-10
089-50
090-100
085-1
086-2
087-5
088-10
089-50
090-100
109-1
110-2
111-5
112-10
113-50
114-100
Weight
(yg/cm )
1.11
2.22
5.55
11.1
55.5
111
1.71
3-43
8.56
17.1
85.6
171
5.5
11.0
27.4
54.8
274
549

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TECHNICAL REPORT DATA "1
(f leave read Instructions on the reverse before completing) I
1 REPCRT NO. !2 " ' 	 ' 	 " 	
EPA-600/2-78-197 !
4, T T^E AND SUBTITLE
CALIBRATION STANDARDS FOR X-RAY SPECTROMETERS USED
FOR POLLUTION SAMPLE ANALYSIS
7. ACTHOR(S)
F.H. Jarke, 0. Puretz, R.A. Semmler, and R.G. Draftz
9 PERFORMING ORGANIZATION NAME AND ADDRESS
I IT Research Institute
10 West 35th Street
Chicago, Illinois 60616
12. SPONSORING AGENCY NAME AND ADDRESS
Environmental Sciences Research Laboratory - RTP, NC
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
3. RECIPIENT'S ACCESSION NO. 1
5. REPORT DATE
September 1978
6. PERFORMING ORGAN 1 ZATI ON CODE
8. PERFORMING ORGANIZATION REPORT NO.
10. PROGRAM ELEMENT NO.
1AD712 BD-07(FY-76)
11. CONTRACT/GRANT NO.
68-02-1734
13. TYPE OF REPORT AND PERIOD COVERED
Final 8/75 - 3/77
14. SPONSORING AGENCY CODE
EPA/600/09
15. SUPPLEMENTARY NOTES
6. ABSTRACT
      A technique  is  described for making aerosol standards for x-ray fluorescence
  analysis by depositing  sized particles suspended in a carrier solution onto  the
  surface of a  polycarbonate filter.  Size is controlled by a separate sedimentation
  step following  grinding in a boron carbide mortar and pestle.  Binding of  the
  deposition to the filter is accomplished by a collodion film layer applied both
  before and after  the particle deposition.  The deposited mass is determined
  gravimetrically from a  companion filter prepared from a large volume aliquot of
  carrier solution  and without collodion.  Standards for 18 different elements have
  been prepared.
17. KEY WORDS AND DOCUMENT ANALYSIS ~~ |
a. DESCRIPTORS
Air pollution
Aerosols
Calibrating
*Stcmdards
Xray spectrometers
Xray fluorescence
18. DISTRIBUTION sTATEMtiN i
RELEASE TO PUBLIC
b. IDENTIFIERS/OPEN ENDED TERMS

19. SECURITY CLASS (This Report)'
UNCLASSIFIED
20. SECURITY CLASS (This page)
UNCLASSIFIED
c. COSATI Field/Group 1
13B
07D
14B
20F
21. NO. OF PAGES
51 1
22. PRICE
    rm 2220-1 (Rev. 4-77)   PREVIOUS EDITION is OBSOLETE

                                          43

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