Unmd States SR-101B
Environmental Protection
Agency
Research and
Development
INDEX AND ABSTRACTS TO PUBLICATIONS
THE EPA-NCI PROJECT: EFFECTS OF
CARCINOGENS, MUTAGENS, AND
TERATOGENS ON NON-HUMAN SPECIES
(AQUATIC ANIMALS )
Prepared by
Environmental Research
Laboratory
Gulf Breeze FL 32561
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THIS PUBLICATION
THE NATIONAL CANCER INSTITUTE CNd) FUNDED &N EPA STUDY EMTITLEO:
"EFFECTS OF CARCINOGENS, MUTAGENS AND TERAT03ENS ON NDN-HUMAN SPECIES
CA.JUATIC A-slIMAuS)" FROM 19?B TO 1932, ASSOCIATED WITH THIS EFFORT WERE
3R. H. K. ADDHIONALL Y , AN AUTHOR
INDEX IS PROVIDED CSEE PAGE S4).
QUESTIONS CDNCERNINi THE CRGJ£CT SHOULD B£ ADDRESSED TC OS. J. COUCH
CC3MMERCIAL 90*-332-5311 OR FTS 6£f-9011). REPRINT OR REPORT REQUESTS
SHOULD c~z DIRECTED TO MS, SUSAN M'ANS COMMERCIAL 904-932-5311 OR
FTS 636-5011). THIS °JBLICATION SUPERSEDES SR-103 AND S3-101A.
HENRY F. 5NQS
LABORATORY DIRECTOR
PREPARATION DATE:
DCTC3ER, 1984
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TABLE OF CONTENTS PAGE
ASOUT THIS PUBLICATION..., .. i
TITLES AND ABSTRACTS... 1
KEYWORD TITLE INDEX 48
AUTHOR INDEX........ 64
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ANDERSON, ROBERT S., JESSICA E. DOGS, AND FRANCIS L. 30SE. 1982..
DIFF£R£NTIAL ABILITY OF AMBYSTOMA TIGRINU"! HEMATIC MICRQSO.MES FROM PCLYCYLlC
AROMATIC ^DROCARBONS AMD AROMATIC AMINES. C*NCE» LETT. 16(l):33-42.
CERL.GB X
A NUM3ER OF CARCINOGENIC AROMATIC AMINES* WHEN ACTIVATED BY LIVER
MICRGSDMES rRDM A SALAMANDER, AMBYSTOMA TIGRINUM, ARE MUTAGENIC FOR
SALMONELLA TESTER STRAINS SENSITIVE TO FRAMESHIFT MUTAGENS. HOWEVER, 2
POLYCY:LIC ARDMATIC HYDROCARBONS CP&H) CBENZOCAJPYRENE CBAP) AND
PERYLE'JE) THAT ARE RENDERED MUTAGENIC SY MAMMALIAN MICRQSOMES ARE NOT
ACTIVATED BY AMBYSTOMA MIXED-FUNCTION OXID4SES. 3AP WAS CHOSEN FOR
STUDY BECAUSE IT IS A WELL-KNOWN ENVIRONMENTAL CARCINOGEN? PERYLENE,
AN IS3MER OF BAP, HAS BEEN IMPLICATED AS AN ETTOLOGICAL AGENT IN
CUTANE3US NE3PLASIA IN AMBYSTOMA. THESE RESULTS SUPPORT THE
OBSERVATION THAT AMPHIBIANS ARE QUITE RESISTANT TO PAH CARCINOGENESIS
AND SUGGEST THAT AROMATIC AMINES MAY 3E MORE APPROPRIATE MODEL
CARCINOGENS.
ANDERSON, ROBERT S. 1978. BENZCC AJPYRENE METABOLISM IN THE AMERICAN OYSTER
CXASSDSTREA VIRGINICA, EPA-6CO/3-73-Q09 , U.S. ENVIRONMENTAL PROTECTION
AGENCY, ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, FL. 19P.
THIS RESEARCH PRDGRAM WAS INITIATED WITH THE OVERALL OBJECTIVE OF
DETERMINING THE RDLE OF NAD?H -DEPENDENT MICROSDMAL MONQ-QXYGE^ASE IN
THE META3CLISM OF THE WIDESPREAD ENVIRONMENTAL CARCINOGEN
BENZOCA5PYRENE <8P> BY THE 3YSTER CRASSQSTREA VIRGINICA. THIS ENZYME
SYSTEM IS IMPORTANT IN DETOXIFYING VARIOUS X3NOBIOTICS AND IN
ACTIVATING POLYCYCLIC AROMATIC HYDRCCARBON ONCOGENS AS BP. A SENSITIVE
RADI3ISOTOPIC SYSTEM WAS DEVcLDPED TO PERMIT THE 3UANTIFIC ATION OF
ALKALI-SOLUBLE ANC WATER-SOLUBLE BP METABOLITES PRODUCED 3Y OYSTER
MQNQ-CXYGENSSE. 4N NADPH- AND OXYGEN-DEPENDENT ARYL HYDROCARBON
HYDRDXYLASE (AHH) WAS SHOWN TD 3E LOCATED IN THE DIGESTIVE GLANDS OF
THESE BIVALVES ASSOCIATED WITH THE MICROSDMAL SU3CELLULAR FRACTION.
THE SPECIFIC ACTIVITY OF OYSTER AHH was CDNSIDERABLY LOWER THAN THAT
OF LABORATORY MICE, 5UT WAS CONSISTENTLY DEMONSTRABLE. THE BP
META30LITES PRODUCED WERE PRIMARILY WATER-SOLUBLE DERIVATIVES. THERE
WAS SOME INDICATION THAT OYSTER AHH WAS INDUCED BY CHRONIC EXPOSURE OF
THE ANIMALS TO THE ENVIRONMENTAL CARCINOGENS BP AND
3-METhYL-CHOLANTHR=NE. THERE WAS STRONG EVIDENCE THAT EXPOSURE TO
POLYCHLOSINATED 3IPHENYLS CPCB) CAUSED AHH INDUCTION. HIGH-PRESSURE
LIQUID CHROMATDGRAPHY WAS USED TO IDENTIFY BP METABOLITES PRODUCED BY
OYSTER AHH. THE GENERATION OF VARIOUS DIHYDRODIQL, QUINONE, AND
HYDRDXY EP DERIVATIVES WAS SHOWN? THIS PRODUCTION WAS AUGMENTED IN
PC8-EXDDSEO OYSTERS. THIS REPORT COVERS TH= PERIOD FROM JULY 1, 1976
TO JUNE 30, 1977, AND WORK WAS COMPLETED AS OF AUGUST 31, 1977.
PAGE
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BIBA, DIANE MARY. IS83. EFFECTS OF AFLATOXIN ON THE B30WN BULLHEAD
ICTALURUS NE3UL03IS. M.S. THESIS. AUBURN UNIVERSITY, AU3URN, AL. 53P.
C£RL,GB X357).
BROWN 3ULLHEADS HAD RENAL LESIONS 25 DAYS AFTER A ONE-HOUR EXPOSURE TO
1.0 M3G/LITER AFLATOXIK B-l CAF3) DURING Th£ FIFTH DAY OP EMBRYONIC
DEVELOPMENT, INDICATING A SIMILARITY TO THE EFFECTS OF AFB REPORTED
FOR RAINBDW TRDUT. RENAL LESIONS WERE NOT FOUND IN BROWN BULLHEADS
AFTER EMBRYO EXPOSURE TD 0.5 MS/LITER AF8. LIV5R HISTOLOGY AND
ULTRASTRUCTURE OF CONTROL BROWN BULLHEADS WERE SIMILAR TO CHANNEL
CATFISH, ICTALURUS PUNCTATJS. NO LESIONS WERE OBSERVED - IN BROWN
3ULLHEAD LIVERS EXAMINED 25 OAYS TO 'EIGHT MONTHS AFTER EMBRYO EXPOSURE
TO 0.5 MG/LITE3 OR 1.0 MG/LITER AF3. THE LACK OF LIVER TUMOR INDUCTION
EIGHT MONTHS AFTER EMBRYO EXPOSURE TO 0.5 MG/LITER AFB OR 1.0 MG/LITER
AF3 SUGGESTS THAT LONGER INDUCTION TIMES OR HIGHER DOSAGES OF AFB ARE
REQUIRED FOR TJM3RIGENICITY IN BROWN BULLHEADS. A 3ENZQCADPYRENE CSAP)
ASSAY INDICATED MO INDUCTION OF THE MlXED-FuNCT ION OXIDASE CMFQ)
SYSTEM IN BROWN BULLHEAD MICROSOMES EIGHT MONTHS AFTER EMBRYO EXPOSURE
TO 0.5 MG/LITER A^B. LESIONS WERE NOT OBE3VSO IN LIVERS OR TRUNK
KIDNEYS OF 3ROWM BULLHEADS 25 DAYS AFTER * ONE-HOUR EXPOSURE DURING
THE FIFTH DAY OF DEVELOPMENT TO AN ORGANIC CONCENTRATE OF EFFLUENT
FROM THE SOUTH TUSKEGSE WATER POLLUTION C3NTROL PLANT, MACQN COUNTY,
ALABAMA. AFTER TH.REE INTRAPER ITCNEAL INJECTIONS OF 1.7 MG/KG AFB AT
10-DAY INTERVALS, LIVERS 0= 3RDWN BULLHEADS EXHIBITED LIMITED
INDUCTION OF THE N"=0 SYSTEM AFTER A 3AP METABOLISM ASSAY, THE MFC
INDUCTION WAS PRQ3A3LY RESTRICTED BY AFLATOXIN INHIBITION OF PROTEIN
SYNTHESIS. INJECTION OF AF3 CAUSED ACCUMULATION OF LIPG^UCHSIN AMD
HEMOSIDERIN wIThIN MiCROPHAGE CENTERS IN THE LIVER, AND
ULTRASTR'JCTURALL*, MITOCHOND.RIAL PfKNOSIS AND VESICULATION AND
OEGRANULATICN DF ROUGH ENDOPLASMIC RETICULJM OF HEPATOCYTES.
BUNTING, DIANE LEIGH. 1979. EVALUATION OF BENZOCA )P YRENE METABOLISM IN AN
OYSTER COSTREA EDULIS5-3ACTERIA SYSTEM, M.S, THESIS. OREGON STATE
UNIVERSITY, CORVALLIS, DR. 121^>.
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COUCH, JOHN A,, AND ELSAYED ELNENAEY.. IN PRE?. COMPLEX CHRCMATQPHORQMA IN A
MARINE TELECST FISH, «=UNDULUS GRANDTS: MORPHOLOGICAL AND BIOCHEMICAL
CHARACTERISTICS. CERL,GE 236).
TH.REE SPECIMENS OF FL^QULUS JRANDIS, THE GUL= KILLIFISH, HAD PIGMENTED
TUMORS COMPRISED OF A CELL TYPE THAT HAD CHARACTERISTICS Qp DIFFERENT
PIGMENT CELL PHENDTYPES. THE PI5H WERE FR3K A MARICULTURE ATTEMPT ON
THE GULF CQA5T 0= ALABAMA USA, AND WERE OLDER LARGER SPECIMENS OF OVER
6,000 F. GRANDIS EXAMINED, A DETAILED LILGHT MICROSCOPY, ELECTRON
MICROSCOPY, CHRQM4TOG3APHIC, AND CELL CULTURE WAS CONDUCTED ON THE
TUMORS FROM THRcE FISH. THE TUMOR CELL POSSESSED PTERINDSOMES AS THE
DOMINANT.CYTGPLAMIC ORGANELLE, BUT ALSO HAD PREMELANOSOMES,
MELANOSQMES, AND POSSIBLE REFLECTING PLATELETS. THE MAJOR PIGMENTS
ISOLATED AND IDENTIFIED WE3E PTERIDINE PIGMENTS. NO CAROTENOID
PIGMENTS OR CAROTENOID VESTICLES WERE IDENTIFIED OR DETECTED. THE
TUMORS WERE INVASIVE, REPLACED AND ISOLATED NORMAL MUSCLE TISSUES, BUT
WERE MOT YETASTATIC. ONE FISH WITH THE TUMORS BECAME MORIBUND WHILE
HELD IN AQUARIUM, AND PRESENTED EVIDENCE THAT THE NEOPLASMS GREW AND
INCREASED IN NUMBERS. THE CAUSES OF THE NEOPLASM IN FUNDULUS ARE
UNKNOWN, BUT BOTH ENVIRONMENTAL AND GENETIC FACTORS MAY PLAY ROLES IN
ITS GENESIS.
COUCH, JOHN A., AND CLYDE DAWE, IN PRESS. MOUSE VS MINNOW: THE FUTURE OF
FISH IN CARCINOGENICITY TESTING (A FRIENDLY DISCUSSION/DEBATE BY DR. JOHN
COUCH AND DR. CLYDE DAWE) - DEBATE: THE FISHY SIDE. IN: PROCEEDINGS OF THE
SYMPOSIUM ON THE USE OF SMALL FISH SPECIES IN CARCINOGENCITY TESTING,
BETHESDA, MARYLAND, DECEMBER 3-10, 1981. U.S. MATIONAL CANCER INSTITUTE.
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COUCH, J3HN A., w. PETER SCHQ3R, LEE C3URTNEY, ANO WILL DAVIS. 1984.
EFFECTS OF CARCINOGENS, MUTAGENS, AND TERATQGENS OF NONHUMAN SPECIES-AQUATIC
ANIMALS (ABSTRACT). IN: PR03RAM & ABSTRACTS THIRD NCI/EPA/NIQSH
COLLABORATIVE WORKSHOP: PROGRESS DM JOINT ENVIRONMENTAL AND OCCUPATIONAL
CANCER STUDIES, MARCH 22-23, 1984, BETHESDA, MO. U.S. NATIONAL CANCER
INSTITUTE, BETHESDA, MD. PP. IV-2-3. (ERL.G3 XV75).
AQUATIC SYSTEMS AND ORGANISMS HAVE BEEN JNDER BDTH LABORATORY AND
FIELD STUDY IN ORDER TO DEVELOP INDICATOR, SCREENING, AND MODELING
CAPABILITIES =OR DETECTION AND EVALUATION! OF RISKS 3F CARCINOGENS,
MUTAGENS, AND TERATOGENS. STUDIES INCLUDE ?3TH GULC BREEZE LABORATORY
PROJECTS AND COMPLEMENTARY, EXTRAMURAL COOPERATIVE AGREEMENTS. DURING
THE CCJRSE CF THE PROGRAM, SEVERAL ADVANCEMENTS HAVE BEEN HADE IN THE
DEVELOPMENT OF LABQRAT3RY AND FIELD CARCINOGEN ASSAY METHODS UTILIZING
FISHES SUCH AS THE SHEEPSHSAD MINNOW (LIVER LESI3NS VIA BEMZIDIN5 AND
AFLATGXIN EXPOSURES), 3IVULUS MARM3RATUS (LIVER LESIONS VIA AFLATOXIN
EXPOSURE), MtNJDIA PENIN3ULAE (LIVER TUMOR INDUCTION WITH AFLATOXIN
EXPOSURE), THE RAINBOW TROUT CTUMOR INDUCTION WITH BENZO(A)PYRENE
(B(A)P) AND METHYL AZOXY^ETHAMOL ACETATE EXPOSURES!, AND FRESHWATER
CATFISH (PAPILLCMAFOUS-LIKE LESIONS VIA CHLORINATED EFFLUENT
EXPOSURES). EMPHASIS HAS SEEN PLACED ON THE DEVELOPMENT AND
UTILIZATION GF CRITICAL LIFE STAGE EXPOSURES (E.G., EMBRYO AND NEWLY
HATCHED FRY EXPOSURES) IN ORDER TO EXPEDITE CARCINOGEN TESTS AND
MINIMIZE TIME REQUIRED FOR DETECTABLE TUMQROGENIC RESPONSES.
PRENEQPL4STIC "STATIC LESION DEVELOPMENT IN MENIDIA NOTED AT 12 WEEKS
AND FDLLOWEO BY HEPATIC TUMORS AT 11 MONTHS, HAS SHOWN PROMISE FOR
THIS SPECIES AMD EXPOSURE METHOD- THE FIRST HEPATIC TUMORS INDUCED IN
FISHES WITH 30LYCYCLIC AROMATICS (E.G., B(A)P) RESULTED IN THE FURTHER
ADVANCEMENT 3F THE RAINBOW TROUT CARCINOGEM ASSAY SYSTEM. BIOCHEMICAL
STUDIES INVOLVING METABOLISM OF PAH'S (E.G., PERYLENE) BY SKIN AND
LIVER TISSUES OF THE TIGER SALAMANDER (AMBYSTOMA TIG3INUM) HAVE
INDICATED INDUCTION 3F ENZYM? ACTIVITY (MF3 SYSTEM), HOWEVER,
EXPERIMENTAL PE?YLENE EXPOSURES HAVE FAILED TO ELICIT CARCINOGENIC
RESPONSES IN TnIS SPECIES. ADVANCEMENTS HAVE BEEN MADE IN
ELSCTROPHCRESIS AND SERQLCGICAL METHODOLOGY TO STUDY SERUM PROTEIN
CHANGES AND HUMORAL IMMUNE RESPONSES IN NOM-EXPOSEO AND EXPOSED (E.G.,
BENZIDINE) FISHES (E.G., CYPRINODQN VARIESATUS). THESE ADVANCES HAVE
SHOWN PROMISE IN DETECTION 0= PHYSIOLOGICAL RESPONSES TO CARCINOGENIC
EXPOSURE. FJTHERMORE, CONSIDERABLE FIELD MONITORING WORK ON MQLLUSKS
AND CARCINOGENIC PAH'S ALONG THE COAST OF OREGON HAS REVEALED A
POSITIVE CORRELATION BETWEEN PREVALENCE OF CELLULAR PROLIFERATION
DISORD8S IN SHELLFISH AND HIGHER CDNCSNTR4TIQMS OF CERTAIN PAH'S IN
NATURAL WATER. EMPHASIS IN BIOCHEMISTRY HAS SEEN DIRECTED MOSTLY
TOWARD THE ELUCIDATION 3F THE METABOLISM 0?= THE MIXED-FUNCTION
OXIDASES IN MARINE ORGANISMS.
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COUCH, J.A. 1984. HIST3PATHOLOGY AND ENLARGEMENT OF THE PITUITARY OF A
TELEOST EXPOSED TO THE HERBICIDE TRIFLURALIN. J, FISH DISEASES.
7(2):157-163. CERLtGB 438).
PITUITARY GLANDS OF SHEEPSHEAD MINNOWS, CYPSINOOON VARIEGATUS, EXPOSED
FOR 19 MONTHS TO 1-5 UG/L TRIFLURALIN WERE SIGNIFICANTLY ENLARGED AND
POSSESSED HIST3PATHQLOGIC CHARACTERISTICS (WHEN COMPARED TG GLANDS OF
CONTROLS) SUCH AS PSEUOQCYSTS, CONGESTION 3F BLOOD VESSELS AND EDEMA.
MOST OF THE FISH WI^H ENLARGED PITUITARIES ALSO HAD DIFFUSE VERTEBRAL
HYPdROSTOSIS AND OTHER DYS^LASTIC VERTEBRAL CHANGES. SEVERAL
- SPECULATIVE MECHANISTIC PATHS ARE SUGGESTED ?QR THE MODE OF EFFECT OF
TRIFLURALIN DN THE VERTEBRAL AND PITUITARY TISSUES- STUDY OF THE FORM
AND FU^CTIDN DP PITUITARY GLANDS OF TELEOSTS FROM NATURAL POPULATIONS
MIGHT PROVIDE INDICATIONS OF CHRONIC PHYSIOLOGICAL STRESS*
PARTICULARLY IN RELATION TD CHEMICAL POLLUTANT STRESS.
C3UCH, JOHN A., W. PETEP SCH03R, WILL DAVIS, AND LEE COURTNEY. 1983.
EFFECTS OF CARCINOGENS i MUTAGENS, AND TERATOGENS ON NQNHUMAN SPECIES CAQUATIC
ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM.
EPA-600/9-83-005, U.S. ENVIRONMENTAL PROTECTI3N AGENCY, ENVIRONMENTAL
RESEARCH LABORATORY, GULF BREEZS, FL. 46?,
AQUATIC SYSTEMS AND ORGANISMS ARE UNDER BOTH LABORATORY AND FIELD
STUDY IN 3RD5R TO DEVELOP INDICATOR, SCREENING, AND MODELING
CAPABILITIES FOR DETECTION AMD EVALUATION OF RISKS QF CACINOGENS,
MUTAGcMS, AND TERATOGENS. STUDIES INCLUDE BOTH GUL- 3REEZE LABORATORY
PROJECTS AND COMPLEMENTARY, EXTRAMURAL PROJECTS. IN THE FOURTH YEAR OF
THE PROGRAM, SEVERAL ADVANCES WERE MADE IN THE DEVELOPMENT OF
LABORATORY AND FIELD CARCINOGEN ASSAYS, UTILIZING FISHES SUCH AS THE
SHEEPSrlEAD MINNOW (LIVER LESIONS VIA AFLATOXIN EXPOSURES), AND
FRESHWATER CAT FISH CPAPILLOMATOUS-LIK? LESIONS VIA CHLORINATED
EFFLUEMT EXPOSURES), EMPHASIS IS STILL PLACED ON THE DEVELOPMENT AND
UTILIZATION OF CRITICAL LIFE STAGS EXPOSURES CE.G., EMBRYO AND NEWLY
HATCHED FRY EXPOSURES) IN ORDER TO EXPEDITE CARCINOGEN TESTS AND
MINIMIZE TIME REQUIRED FOR TUHOROGENIC RESPONSES. PRENEOPLASTIC
HEPATIC LESI3N DEVELOPMENT IN MENIDIA AT 12 WEEKS SUGGESTS PROMISE FOR
THIS SPECIES AND EXPOSURE METHOD. A NOVEL APPROACH HAS SHOWN THAT
TIGER SALAMANDERS MAY BE GOOD BIOCHEMICAL AND HISTQLOGIC INDICATORS OF
THE PRESENCE DF CERTAIN CARCINOGENS CPOLYCYCLIC AROMATIC HYDROCARBONS
- PAH'S). SKIN AND LIVER TISSUES -3F THE SALAMANDERS REVEALED INDUCED
ENZYME ACTIVITY (M=0 SYSTEM) FOLLOWING EXPOSURE TO THE PAH, PERYLEN5.
CONSIDERABLE FIELD MONITORING WORK ON MOLLUSKS AND CARCINOGENIC PAH'S
ALONG THE COAST OF OREGON HAS REVEALED A POSITIVE CORRELATION BETWEEN
PREVALENCE OF CELLULAR PROLIFERATION DISORDERS IN SHELLFISH AND HIGHER
CONCENTRATIONS OF CERTAIN PAH'S IN NATURAL WATER.
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COUCH, JOHN A. 1982. AQUATIC ANIMALS AS INDICATORS OF ENVIRONMENTAL
EXPOSURES. J. ENVIRON. SCI. HEALTH PART A ENVIRON. SCI. 6NG- 17<4):473-476.
CERL.GB 431*).
AQUATIC ANIMALS ARE USEFUL AS INDICATORS 3P MiNY KINDS OF POLLUTANTS
IN THE A3UATIC ENVIRONMENT, THE ORESSNCE 0= PO-.LJTANTS IN THE GENERAL
ENVIRONMENTS 0^ AIR, LAND AND WATER IS REFLECTED IN THEIR
ACCUMULATIONS IN AND EFFECTS QN AQUATIC OR3ANISMS BECAUSE THE AQUATIC
PORTION OF THE BIOSPHERE IS OFTEN THE "SINK" FOR HUMAN-GENERATED
POLLUTANTS. AQUATIC ANIMALS LENO THEMSELVES PARTICULARLY WELL TO THE
STUDY OF SPECIAL PHENOMENA, SUCH AS CARCINOGENESIS AND TERATQGENESIS.
THE USE OF AQUATIC ANIMALS AND SYSTEMS ADDS AN IMPORTANT DIMENSION TO
RESEARCH ON THOSE POLLUTANTS THAT MAY AFFECT WILDLIFE AND HUMAN
HEALTH.
COUCH, JOHN A., AND W. PETER SCHOOR. 1982. EFFECTS OF CARCINOGENS,
MUTAGEN5, AND TdRATOG5NS ON NON-HUMAN SPECIES CAQUATIC ANIMALS); IN:
PROCEEDINGS OF THr SECOND NCI/EPA/NIOSH COLLABORATIVE WORKSHOP: PROGRESS ON
JOINT ENVIRONMENTAL AND OCCUPATIONAL CANCER STUDIES, SEPTEMBER 9-11, 1981,
ROCKVILLE, MD. HERMAN F. KRAYSILL, INGE30RG C. BLACKWOCO, AND NANCY 8.
FREAS.EOITORS, U.S. GOVERNMENT PRINTING OFFICE, WASHINGTON, DC. ?P. 688-726.
CERL.GB 433*).
AQUATIC SYSTEMS AND ORGANISMS ARE UNDER EXAMINATION IN ORDER TO
DEVELOP INDICATOR, SCREENING, AND MODELING CAPABILITIES FOR DETECTION
OF CARCINOGENS, MUTAGEMS AND TERATQGENS. IN THE THIRD Y=AR OF THE
PROGRAM, SEVERAL ADVANCES WERE MADE IN THE DEVELOPMENT OF ASSAYS,
UTILIZING SHEEPSHEAD MINMCWS CLIVER LESIONS VIA 8ENZICINE AND
AFLATOXIN EXPOSURE), RAIN3CW TROUT (LIVER TUMORS VIA 8ENZOCA)PYREN£
EXPOSURES). RESULTS OF LONG-TERM EXPOSURES OF FISH TO THE HERBICIDE
TRIFLU3ALIN SHOW THAT INDUCED 3QNEY GROWTHS ARE ACCOMPANIED 9Y
HISTOPATHOLOGIC CHANGES OF THE PITUITARY. THESE AND OTHER STUDIES
CONTINUE TO REVEAL THAT FISH HAVE META30LIC PATHWAYS SIMILAR TO
MAMMALS FOR DISPOSITION OF CERTAIN CARCINOSENS. A SIGNIFICANT NUMBER
OF COOPERATIVE AGREEMENTS WITH PRINCIPAL INVESTIGATORS SUPPORT AN
EXTRAMURAL COM?LEMENTAL AND SUPPLEMENTAL EFFORT IN THE IDENTIFICATION
OF AQUATIC SPECIES AND SYSTEMS THAT MAY SERVE AS EARLY WARNING
MECHANISMS.
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C3UCH, JOHN A., AND W. PETER SCHODR. 1981. EFFECTS GF CARCINOGENSt HUTAGENS
AND TERATDGENS ON NON-HUMAN SPECIES-AQUATIC ANIMALS. IN: PROCEEDINGS OF THE
FIRST NCI/EPA/NIQSH CDLLA803ATIVE WORKSHOP: °RQGRESS CN JOINT ENVIRONMENTAL
AND OCCUPATIONAL CANCER 5TUDIE5. H.=. KRAY6ILL, INGE3DRG C. BLACKWOOD, AND
NANCY 3. FR.EAS, EDITORS, U.S. GOV'RNM^NT PRINTING OFFICE, WASHINGTON, DC-
PP. 333-376. CERLiSB X332).
A RESEARCH PROGRAM USING A2UATIC SYSTEM 4ND ORGANISMS TO* STUDY THE
FATE AND EFFECTS IF CARCIN035MS IM THE AQUATIC ENVIRONMENT HAS 3EEN
UNDERWAY DURING THE iFY'S 78 AND 79 AT THE 3ULF BREEZE EPA LABORATORY.
THE TW3 MAJOR INVESTIGATIVE, DISCIPLINARY 4REAS HAVE BEEN IN
PATHOBIOLOGY AMD BIOCHEMISTRY. A FISH-CARCINOGEN ASSAY SYSTEM HAS SEEN
DEVELOPED THAT INVOLVES LABORATORY CONTROLLED LQN3-TERM EXPOSURE OF
FISH TO SUSPECT CARCINOGENS FOLLOWED BY HISTTPATHCLQGY AND PHYSIOLOGY
OF EX=OSED CISH, INDUCED 3RJWTHS, AND RELATED DISORDERS. THIS SYSTEM
APPEARS ADEQUATE FDR CARCINOGEN TESTS =OR FISH. A TWO YEAR FIELD STUDY
IS UNDERWAY OF TUM03, DISEASE PREVALENCE, AND CARCINOGEN RESIDUE OR
METABOLITES IN FISH AND SHcLLFlSri IN VARIOUSLY POLLUTED ESTUARIES
ALCNG THE NORTHERN GULF OF MEXICO. IT IS TOO EARLY TO PREDICT THE
SIGNIFICANCE OF THE FINDINGS IN THIS STUDY-* 3UT SEVERAL NEW TUMOR
TYPES HAVE BEEN DISCOVERED IN FISH. BIOCHEMICAL STUDIES HAVE REVEALED
TH«T FISH MAY RESPOND IN ENZYMATIC REACTIONS IN WAYS SIMILAR TO
MAMMALS EXPOSED TO THE SAME CARCINOGENS, AMD THAT FISH MAY PROVE TO 3E
ADEQUATE SUPPLEMENTAL, BIOLOGICAL MONITORS DF CARCINOGENS IN THE
ENVIRONMENT,
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COUCH, J3HW 4., AND W.P. SCHGOR. 1931," EFFECTS OP CARCINOGENS, MUTAGENS,
AND TERATOGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS). IN: SECOND ANNUAL
REPORT ON NATIONAL CANCER INSTITUTE AND ENVIRONMENTAL PROTECTION AGENCY
PROJECTS. U.S. OEPT. 0~ HEALTH, EDUCATION, AND WELFARE, PUBLIC HEALTH
SERVICE, NATIONAL INSTITUTE OF HEALTH, BrTHESDA, MD- PP. 57-103.
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COUCH, JOHN A., LEE 4. COURTNEY, AND STEVEN S. FOSS. 1981. LABORATORY
EVALUATION OF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS. IN: PHYLETIC
APPROACHES TO CANCER: PROCEEDINGS OF THE 11TH INTERNATIONAL SYMPOSIUM OF THE
PRINCESS TAKAMATSU CAMCER RESEARCH =UMD, TOKYO, 1980. CLYDE J. DAWE, EDITOR,
JAPAN SCIENTIFIC SCIENCES P3ESS, TOKYO, JAPAN. P?. 125-139. CERL,GB 171*).
THE U.S. E=A AND THE NATICNAL CANCER INST. CNCI) HAVE MAJOR
RESPONSIBILITIES FOR DETERMINING THE 'ATc AND RISKS OF CARCINOGENIC
AGENTS IN THE NATURAL ENVIRONMENT. UNDER THE AUSPICES OF EPA/NCI, THE
CARCINOGEN RESEARCH TEAM AT THE USEPA LAB., GULF 3RF.EZE, HAS A MAJOR
ROLE IN INVESTIGATING THE FATE, EFFECTS, AND RISKS 3F CARCINOGENIC
AGENTS IN THE AQ'JATIC PORTION OF THE BIOSPHERE. IN REGARD TO THIS
RCLE, THERE IS A NEED FOR PRACTICAL, EXPERIMENTAL EXPOSURE SYSTEMS FOR
THE SHORT TERM, ANO LONG TERM EXPOSURE OF FISHES AMD INVERTEBRATES IN
ORDER TO EVALUATE THEIR RESPONSES TO ENVIRONMENTALLY SIGNIFICANT
CARCINOGENS. WE HAVE DESIGNED AND TESTED AN ADAPTABLE, AQUATIC
LABORATORY SYSTEM FOR FLOWING WATER OR STATIC WATER ASSAYS OF
CARCINOGENIC OR SUSPECT CARCINOGENIC AGENTS AGAINST MARINE FISHES. WE
REPORT HE3E THE DESIGN, RESULTS DF LING TE3M TESTS, SND THE FUTURE
USES OF THE SYSTEM FDR DETERMINING THE RISKS 0*= CARCINOGENIC AGENTS IN
THE AQUATIC ENVIRONMENT, AND AS A SYSTEM COMPLEMENTARY TO MAMMALIAN
ASSAY SYSTEMS, BUT WHICH PERMITS THE PHYLOGENETIC EXPANSION OF
CARCINOGEN ASSAY METHODOLOGY, A PILOT TEST OF THE DESCRIBED SYSTEM HAS
BEEN COMPLETED. THIS TEST UTILIZED FLOWING FILTERED, ESTUARINE WATER,
CONTROLLED «IATER TEMPERATURE, CONTROLLED PHOTO PERIOD, CONTROLLED
NUTRITION OF TEST SPECIES, OXYGEN CONCENTRATION MONITORING, AND
VARIOUS LIFE CYCLE STAGES OF THE TEST FISH, THE SHEEPSHEAD MINNOW,
CYPRINDDON VARIEGATUS, AND THE SUSPECT CARCINOGENIC HERBICIDE,
TRIFLURALIN, CONTINUOUS EXPOSURES TO 1 TO 5 MG/L T'RI^LURALIN WERE
CONDUCTED WITH ZYGOTE THRU ErtBRYOGENSSIS TO ADULT STAGES OF THE FISH.
COUCH, JOHN A. 1981. VIRAL DISEASES OF INVERTEBRATES OTHER THAN INSECTS.
IN: PATHDGENESIS 0= INVERTEBRATE MICROBIAL DISEASES, ELIZABETH W. DAVIDSON,
cOITOR, ALLENHELD, QSMUN, TOTCWS, NJ. PP. 121-160. CERL.G3 274).
THIRTEEN EXAMPLES OF VIRUS OR VIRUSLIKE RELATED PATHOSES IN NON-INSECT
INVERTEBRATES ARE DESCRIBED. FROM CONSIDERATION OF THESE EXAMPLES, IT
BECOMES 03VIQUS THAT DETAILED DESCRIPTIONS OF PATHOGENESIS OF VIRUS
DISEASES IN NON-INSECT INVERTEBRATES HAS NOT KEPT PACE WITH THE
FREQUENCY OF NEW REPORTS OF VIRUSES FROM THESE INVERTEBRATES. WHEN
POSSIBLE, IT IS Or UPMOST IMPORTANCE FOR AUTHORS REPORTING VIRAL
ENTITIES -ROM INVERTEBRATES TO INCLUDE AS MUCH INFORMATION AS CAN BE
COLLECTED ON THE DISEASE SYNDROME, CELLULAR PATHOGENESIS,
PHYSIOLOGICAL AND BEHAVIORAL EFFECTS AND TISSUE DISTRIBUTION OF THE
VIRUS. IN SOME CASES, INFORMATION HAS BEEN SO SCANT THAT READERS ARE
NOT EVEN CERTAIN A3 TO THE PREVALENCE OF THE VIRUS IN REPORTED HOSTS.
BASIC INFORMATION ON THE MATURE C<= NON-INSECT INVERTEBRATE VIRUS
DISEASES IS N-EDEO FQ3 SEVERAL REASONS. IMSIGHTS INTO MECHANISMS OF
PATHOGZNCSIS ANG ^ATHOGENIC EFFECTS IM INVERTEBRATE VIRUSES MAY GIVE
BETTER UNDERSTANDING Zf VERTEBRATE VIRUS DISEASES, FURTHER, A MAJOR
OPP3RTJNITY EXISTS WITH INVERTEBRATE VIRUS DISEASE TO INVESTIGATE THE
FUNDAMENTAL ASPECTS OF CELLULAR IMMUNITY,.
PAGE
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COUCH, JOHN A., FRANK G. LOWHAN, AND FORD A. CROSS, 1950. BIOMONITQRING OF
COASTAL WATERS—AN OVERVIEW. IN: BIOLOGICAL MONITORING «=OR ENVIRONMENTAL
EFFECTS. DOUGLAS L. KCRFi EDITOR, 0.C. HEATH AND COMPANY, LEXINGTON, MA.
PP. 93-95. (ERL,3B X155*).
fcSTUARINE AND COASTAL WATERS OF THE UNITED STATES NOT ONLY SERVE AS
PRIME HASITAT FOR A SIGNIFICANT FRACTION OF COMMERCIAL AND
RECREATIONAL MARINE FISHERIES, BUT ALSO RECEIVE INDUSTRIAL AND
MUNICIPAL WASTES FROM A RAPIDLY EXPANDING COASTAL ECONOMY. IN ORDER TO
ENSURE THAT LEVELS OF CONTAMINANTS IN COASTAL ECOSYSTEMS DO NOT AFFECT
EITHER PUBLIC HEALTH OR FISHERIES RESOURCES ADVERSELY, BOTH STATE AND
FEDERAL AGENCIES ARE CONDUCTING A VARIETY OF BIOMONITORING PROGRAMS.
IN ADDITION, GENERIC RESEARCH PROJECTS ARE UNDERWAY AT SEVERAL
UNIVERSITY, STATE, AND FEDERAL LABORATORIES TO DETERMINE WHICH
ENVIRONMENTAL AND PHYSIOLOGICAL FACTORS REGULATE THE 3QDY 8URDEN OF
CONTAMINANTS IN MARINE ORGANISMS. THIS INFORMATION CAN THEN SE USED TO
HELP INTERPRET DATA OBTAINED IN BICMONITORING PROGRAMS AND TO 'ALLOW
MORE PRECISE PREDICTIONS OF CONTAMINANT LEVELS IN BIOTA PRIOR TO
DISCHARGE.
COUCH, JOHN A., AMD W. PETER SCHOOR. 1980. EFFECTS OF CARCINOGENS,
MUTAGENS, AND TESATOGENS ON NON-HUMAN SPECIES CAQUATIC ANIMALS). IN: ANNUAL
REPORT ON NATIONAL CANCER INSTITUTE AND ENVIRONMENTAL PROTECTION AGENCY
PROJECTS. U.S. OE?T. QF HEALTH, EDUCATION, AND WELFARE, PUBLIC HEALTH
SERVICE, NATIONAL INSTITUTE OF HEALTH, BETHESDA, MD. 38P. CERL.GB XQQ2*>.
A RESEARCH PROGRAM USING AQUATIC SYSTEMS AND ORGANISMS TQ STUDY THE
FATE AND EFFECTS C-= CARCINOGENS HAS BEEN UNDERWAY DURING FY 79 AT THE
GULF BREEZE EPA LABORATORY. THE TWO MAJOR INVESTIGATIVE, DISCIPLINARY
AREAS HAVE BEEN IN PATHC3IOLOSY AND BIOCHEMISTRY. A FISH-CARCINOGEN
ASSAY SYSTEM HAS 3EEN DEVELOPED THAT INVOLVES LABORATORY, CONTROLLED
LONG-TERM E RESPONSIBLE FOR PRODUCING ULTIMATE OXIDIZED
METABOLITES.POSU^E 0= PISH TO SUSPECT CARCINOGENS FOLLOWED BY
HISTOPATHOLOGY OF EXPOSED FISH AND THE FINDING QF INDUCED GROWTHS.
THIS SYSTEM APPEARS ADEQUATE FOR CARCINOGEN TESTS FOR FISH. A FIELD
SURVEY IS UNOERWAY FOR TUMOR AND DISEASE PREVALENCE IN FISH AND
SHELLFISH IN VARIOUSLY POLLUTED ESTUARIES ALOMG TH£ NORTHERN GULF OF
MEXICO. IT IS TOO EARLY TO PREDICT THE SIGNIFICANCE OF THE FINDINGS IN
THIS STUDY. BIOCHEMICAL STUDIES HAVE REVEALED THAT FISH MAY RESPOND IN
ENZYMATIC REACTIONS IN WAYS SIMILAR TO MAMMALS EXPOSED TO THE SAME
CARCINOGENS, AND THAT FISH MAY PROVE TO Be ADEQUATE SUPPLEMENTAL,
BIOLOGICAL MONITORS Of CARCINOGENS IN THE ENVIRONMENT. IT WAS SHOWN
THAT ASIDE FRO* INDUCTION OF OXYGENASE ACTIVITY, TRANSFERASES
RESPONSIBLE -0* DETOXIFICATION REACTIONS A3E ALSO INDUCED, THE LATTER
BY META30LITE5 RATHER THAN *A!?£NT COMPOUND. THE SIGNIFICANCE OF THIS
IS THAT WHILE THE OXYGENASES APE RESPONSI3LE FO* PRODUCING ULTIMATE
CARCINOGENS FROM PROCARCINDGENS, THE TRANSFERASES ARE INVOLVED IN THE
EXCRETION AMD HENCE DETOXIFICATION OF THE OXIDIZED METABOLITES.
PASE 10
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COUCH, JDHN A., LES A, COURTNEY, JAMES T, WINSTEAD, AND STEVEN S, FOSS.
1979. AMERICAN OYSTER CCRASSOSTREA VIRGINICA) AS AN INDICATOR OF CARCINOGENS
IN THE AQUATIC ENVIR3NMENT. IN: ANIMALS A3 MONITORS OF ENVIRONMENTAL
POLLUTANTS. NATIONAL ACADEMY OF SCIENCES, WASHINGTON, DC. PP. 65-84.
CERL.GS 338).
THE AMERICAN OYSTER CC. VIRGINICA) WAS USED AS THE EXPERIMENTAL ANIMAL
FOR CHRONIC EXPOSURE TO 3-METHYLCH3LANTHRENE C3-MC) AND 3EMZ3CA3PYRENE
CBP) I.M AN EXPOSURE SYSTEM IN WHICH THE CARCINOGENS CAN 8E
CONTZNJOUSI.Y INJECTED INT3 FREE FLOWING W*T£R AT FIXED RATES RANGING
FROM 1 TO 5 MG/L. EXPERIMENTS DESIGNED TO DETERMINE UPTAKE AND
DISTRIBUTION 3F H3MC AND C14BP SHOWED THAT THESE ARE CONCENTRATED IN
OYSTER TISSUES IN DIRECT PROPORTION TD THE DOSAGE OF CARCINOGEN
INJECTED INTO THE SYSTEM. RESIDUAL CONCENTRATIONS AS HIGH AS 34.4
MG/KG OF MC AND 36,4 MG/KG OF 3P WERE PRESENT IN OYSTERS AS LONG AS 6
MONTHS FOLLOWING EXPOSURE. AUTQRADIOGRAPHY SHOWED INTENSE LOCALIZATION
OF C14BP IN DISTAL PORTIONS 3F THE TUBULES OF THE DIGESTIVE GLAND AND
TO A LESSER EXTENT IN THE GONADAL TISSUES. ARYL HYDROCARBON
HYDRQXYLASE CAHH) ACTIVITY WAS PRESENT IN HQMO&ENATES OF
HEPATQPANCREAS AFTER 5.5 M3NTHS 3r EXPTSURE TC THE CARCINOGENS, IN
CONTRAST TC C3NTRDL ANIMALS IM WHICH AHH ACTIVITY WAS QUITE LOW. IN
EIGHT OYSTERS EXPOSED TO MC, AN INFILTRATION DF CELLS BELIEVED TO BE
OF h£MATQPOI£TlC ORIGIN WAS ENCOUNTERED IN THE MANTLE. SOME APPEAR TO
BE IDENTICAL IN TYPE TO THOSE WHICH CONSTITUTE SARCOMA-LIKE LESIONS
ENCOUNTERED IN FERAL OYSTER POPULATIONS. HOWEVER, IT WOULD BE
PREMATURE AT THIS STAGE TO ASSIGN ANY ETIQLOGICAL SIGNIFICANCE TO THE
EXPERIMENTAL FINDINGS.
C3UCH, JDHN A., AND J4MSS T. WINSTEAD. 1979, CONCURRENT NEOPLASTIC AND
PROTISTAN DISORDERS IM THE AMERICAN OYSTER (CRASSDSTREA VIRSIMICA5.
HALIOTIS. 3C2):2'49-253. (ERL,G3 353*).
AVAIL. FR3M NTIS, SPRINGFIELD, VA: P380-199706.
ONE OF 373 OYSTERS EXAMINED AS PART OF A HISTOLOGICAL SURVEY OF
OYSTERS FROM APALACHICCLA BAY, FLORIDA, USA, HAD & CONCURRENT BLOOD
CELL PROLI-ERATIVE DISORDER AND A PROTISTAN INFECTION. THE NEOPLASTIC
BLOOD CELLS (LEUKOCYTES) WERE FOUND THROUGHOUT THE VESICULAR
CONNECTIVE TISSUES AND 3LOOD SPACES IN SECTIONS OF THE OYSTER. THESE
PROLIFERATING CELLS RESEMBLED THE NEQPLASTIC CELLS DESCRIBED BY COUCH
(1969) AND FARLEY C1369) FRO!J! 3THE3 SPECIMENS 3F CRASS3STREA VIRGINICA
FROM CHESAPEAKE BAY, MARYLAND, USA. MITOTIC FIGURES WERE ABUNDANT IN
FOCI DF THE NEOPLASTIC TISSUE. EPITHELIAL TISSUES OF THE GUT OF THIS
OYSTER WERE IN=ECT5D BY SPORE AND SCHIZOGONIC STAGES OF DERMOCYSTIOIUM
MARINUM, A PRESUMED PATHOGENIC PRCTIST 0? 3YSTERS. SPORES OF THIS
PROTIST AND 3THER PRQLIFERATIVE STAGES WERE OBSERVED IN CONNECTIVE
TISSUES AND 3L30D SPACES, THERE WAS NO MORPHOLOGICAL EVIDENCE THAT THE
NEQPLASTIC BLODD CELLS WERE RELATED TO THE PRQTIST LIFE CYCLE STAGES.
PAGE 11
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COUCH, JOHN A. 1979. SHRIMPS (ARTHROPOD*: CRUSTACEA: PENAEIDAE). IN:
POLLUTION ECOLOGY OF ESTUARINE INVERTEBRATES. C.W. HART, JR. AND L.H.
SAMUEL, EDITORS, ACADEMIC PRESS, NEW YORK, NY. PP. 235-258. (£RL,G5 X046*).
THE MAJORITY OF REPORTS AVAILABLE ABOUT POLLUTION AND PENAEID SHRIMPS
CONCERNS STUDIES INVOLVING THE COMMERCIALLY VALUAPLE PENAEID SHRIMPS
OF THE U.S. ATLANTIC STATES AND GULF COAST. THEREFORE, MOST OF THE
INFORMATION PRESENTED HF8E WILL 3E RELATED TO THE FOLLOWING THREE
SPECIES: PE-MA5US DJ03ARUM CPINK SH5IMP), PENAEUS AZTECUS CBRQWN
SHRIMP), AND PENAEUS SETIFERUS CWHITE SHRIMP), ALL ATLANTIC AND GULF
OF MEXICO SPECIES. REFERENCE TO UTHE3 SPECIES OF PENAEID AND SOME
NONPENAEID CRUSTACEA WILL BE MADE WHEN SPECIFIC STUDIES CONTRIBUTE
SIGNIFICANTLY TO OUR UNDERSTANDING OF POLLUTION ECOLOGY OF SHRIMPS.
THIS CHAPTER WILL COVER THE FOLLOWING POLLUTANT CATEGORIES AND
SITUATIONS: ORGANIC CHEMICALS OTHER THAN PETROLEUM, PETROLEUM AND
RELATED COMPOUNDS, HEAVY METALS, 3IOL3GICAL A3ENT5, AND INTERACTIONS
OF POLLUTANTS AND OTHER FACTORS. UNDER EACH OF THESE DIVISIONS
TOXICITY AND SPECIFIC TISSUE, ORGHNISMIC, POPULATION, AND ECOLOGICAL
£FCECTS WILL BE REVIEWED WHEN KNOWN. FURTHER, THE UPTAKE, TRANSPORT,
AND FATE Or POLLUTANTS WILL 3E DISCUSSED AS THEY MAY AFFECT THE
ECOLOGY OF PENAEID SHRIMPS
COUCH, J.I., J.T. WINSTEAD, D.J. HANSEN, AND L-R. GOODMAN. 1979. VERTEBRAL
DYSPLASIA IN YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN. J. FISH
DISEASES. 2(l):35-42.
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COURTNEY, IE' A., AND JOHN A. COUCH. IN PRESS. USEFULNESS OF CYPRINODON
VARIESATUS AND FUNOULUS GRANDIS IN C ARCINOGEMICI TY TESTING: ADVANTAGES AMD
SPECIAL PROBLEMS. IN: PROCEEDINGS 0= SYMPOSIUM ON THE USE OF SMALL PISH
SPECIES IN CARCINOGENCITY TESTING, SFTHESDA, MARYLAND, DECEMBER 8-10, 1981.
U.S. NATIONAL CANCER INSTITUTE. (ERL,G3
CYPRINDDQN VARIEGATUS AND FUNDULUS GRANDIS, TWO SPECIES OF
CYPRINDDONFID CISHES EXTENSIVELY STUDIED AMD USED IN TOXICOLQGICAL AND
BIOLOGICAL INVESTIGATIONS, ARE COMPARED AS LA30RATORY TEST ANIMALS.
THEIR ECOLOGY AND GENERAL BIOLOGY, AND SUITABILITY FOR VARIOUS TYPES
OF EXPERIMENTATION ARE EXAMINED. A LABORATORY SYSTEM FOR EXPOSING
CRITICAL LIFE STAGES (E.G., EMBRYOS, FRY, JUVENILES) OF THESE SPECIES
TO SUSPECT CARCINOGENS IS DESCRIBED. A DISCUSSION OF THE USE,
FINDINGS, AND POTENTIAL Cc THESE SPECIES IN ONCOLOGICAL STUDIES AND
CARCINOGEN ASSAYS IS PRESENTED, PARTICULARLY IN REGARD TO RESPONSES TO
THREE KNOWN OR SUSPECT CARCINOGENIC CHEMICALS (E.G., TRIFLURALIN,
BENZIDINE, AND AFLATQXIN). FINALLY, ADVANTAGES AND DISADVANTAGES OR
SPECIAL PROBLEMS IN USING THE SPECIES AS CARCINOGEN TEST ANIMALS ARE
REVIEWED.
ELNENAEY, ELSAYED A,, AMD W. PETER SCHOOR, 1931. SEPARATION OP THE ISOMERIC
PHENOLS OF 3ENZOCA)PYRENif 3Y HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. ANAL,
BIOCHEM. lll(2)i393-4QQ. CERL,G3 046*).
AVAIL. FROM NTIS: PB31-233637.
A TECHNIQUE -IAS BEEN DEVELOPED TO SEPARATE A MIXTURE 0= THE TWELVE
ISOMERIC PHENOLS OF BENZOCA)PYRENE BY USING HIGH-PERFORMANCE LIQUID
CHROMATQSRAPHY -IITH FLUORESCENCE DETECTION AND VARIOUS SEQUENCES OF
METHANOL/WATER GRADIENTS. DETECTION BY FLUORESCENCE HAS INCREASED THE
SENSITIVITY OF THE METHOD WHEN COMPARED WITH UV DETECTION. THE EFFECTS
OF FLOW RATE, TEMPERATURE, AND VARIOUS TYPES OF GRADIENTS ON THE
SEPARATION OF THE ISOMERIC PHENOLS ARE DISCUSSED. IT HAS BEEN FOUND
THAT UN.DER THE CONDITIONS NORMALLY EMPLOYED TO SEPARATE THESE PHENOLS
FROM OTHER METABOLITES OF BENZOC A)PYRENE, SEVERAL DF THE PHENOLS ARE
NOT SEPARATED POSSIBLY LEADING TO INCORRECT IDENTIFICATION AS WELL AS
QUANTITATIQN. DIOLS AND OIONES OF BENZO(A) PY RENE ARE ALSO SEPARATED 8Y
THIS PROCEDURE.
PAGE 13
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ELNENAEY, ELSAYEO, AND W.P. SCHOOR. 1981. SIMPLE HIGH PERFORMANCE LIQUID
CHRQMATOGRAPHY METHOD FDR SEPARATION OF BENZOCA)PYRENE.METABOLITES
(ABSTRACT). CLIN. CHEM. 27(6):i099. (ERL.GB X344),
TO CONTINUE OUR STUDY TO DEVELOP A RELIABLE METHOD FOR QUALITATIVE AND
QUANTITATIVE SEPARATION OF BENZQCA)PYRENG (BAP) METABOLITES* US REPORT
A SIMPLE, RAPID HPLC USING PERKIN ELMER QD3 SIL-X COLUMN AND A MIXTURE
OF METHANOL AMD WATER AS IS3C3ATIC ELUANT. AS WE PREVIOUSLY REPORTED
(J. ANAL. 3IQCHEM., IN PRESS) BOTH THE FLOW RATE ANO SOLVENT
COMPOSITION HAVE DIFFERENT EFFECTS ON SEPARATION PATTERN. THE USE OF
30THt FLUORESCENCE AND U.V. DETECTION SIMULTANEOUSLY WAS FOUND TO BE
OF GREAT IMPORTANCE CONCERNING THE FACT THAT SOME METABOLITES E.G.
DIONtS MAY HAVE RELATIVELY GOOD ABSORPTION WHILE THE OTHER METABOLITES
(PHENOLS AND DIOLS) HAVE EXCELLENT FLUORESCENCE ACTIVITY AND
RELATIVELY PODS ABSORPTION. THE ABSORPTION WAS MONITORED AT 254 NM AND
FLUORESCENCE (EX. 363 NM AND EM. 370 FILTER). THE USE OF AM ISOCRATIC
SOLVENT (65? METHANOL IN WATER) ELIMINATES USE OF ANY DRIFT OF THE
BASELINE SPECIALLY F03 THE UV SIDS IF COMPARED TO A GRADIENT SYSTEM.
TH£ FLOW SATES RANGING FROM 0.3-0.6 ML/MIN. WERE TESTED AMD THE 0.4
ML/MIN. RESULTED IN A GOOD SEPARATION. THE FOLLOWING BAP DERIVATIVES
WERE SEPARATED IN ONE CHRQMATOGRAM (T)9,1Q-, (T)7,3-DIHYDRODIOL, 1,6-,
6,12- AND 3,6-DIONE, 4,5-EPOXIOE, 5-OH, 6-3H, 9-OH, 10-OH, 7-QH, 1-OH,
AND 3-OH BAP. /JH = N AM IN VITRO REACTION OF BAP AND LIVER MICROSOMES OF
A TREATED FISH, FUNQULUS GRANOIS, WAS SEPARATED BY THIS METHOD, ALL
THE ABOVE DERIVATIVES, BUT 10-OH, ISOLATED AND IDENTIFIED AS
METABOLITES. THE METHOD IS SIMPLE, EFFICIENT IN RESOLUTION OF THE
METABOLITES WITH N3 POSSIBILITY OF OVERLAPPING PEAKS THAT MAY LEAD TO
INCORRECT IDENTIFICATION AND QUANTITATIDN.
ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, FL . 1984. INDEX AND
ABSTRACTS TO PUBLICATIONS, THE EPA-NCI PROJECT: EFFECTS OF CARCINOGENS,
MUTAGENS, AND TERATDGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS). U.S.
ENVIRONMENTAL PROTECTION AGENCY, ENVIRONMENTAL RESEARCH LABORATORY, GULF
BREEZE, FL. 70?. (ERL,G3 SR-1013),
THE NATIONAL CANCER INSTITUTE (NCI) FUNDED AN EPA STUDY ENTITLED:
"EFFECTS 0= CARCINOGENS, MUTAGENS AND TERATOGENS ON NON-HUMAN SPECIES
(AQUATIC ANIMALS)" FROM 1978 TO 1932. ASSOCIATED WITH THIS EFFORT WERE
OR. H. KRAYBILL (NCI) AND DR. JOHN COUCH (EPA), PRINCIPAL INVESTIGATOR
AND PROJECT COORDINATOR. TO DATE, THE JOINT EFFORT HAS PRODUCED ABOUT
103 REPORTS, SYMPOSIA SECTIONS OR PUBLICATIONS IN THE PEER-REVIEWED,
JOURNAL LITERATURE. CONTRIBUTIONS ARE LISTED, WITH ABSTRACTS (WHEN
AVAILABLE) AND CROSS REFERENCED BY TITLE-KEY WORDS. ADDITIONALLY, AN
AUTHOR IMDEX IS DROVIOED.
GREGORY, PAULA £., ^ATRICIA N. HOWARD-PEEBLES, RUDOLPH D. ELLENOER, AND BILLY
J. MARTIN. 1980. ANALYSIS OF A MARINE FISH CELL LIN- FROM A MALE
SHEEPSHEAD. J. HERtD. 71(3): 209-211. (ERL,GS X193*),
CHROMOSOMES FROM CONSECUTIVE CULTURE PASSAGES OF A DEVELOPING CELL
LINE FROM FIN FI3ROBLASTS OF A MALr SHEEPSHEAD (ARCHOSARGUS
PROBATDCEPHALUS) WERE ANALYZED. IT WAS DEMONSTRATED THAT THE MODAL
CHROMOSOME NUMBER IS 43. THE CHROMOSOME TYPES FOUND IN THESE CELLS
INCLUOcO 8 SU8METACENTRICS, 3-12 ACRQCENTRICS, AND 23-32 TELOCENTRICS.
EVIDENCE SUGGESTS THAT THE PRIMARY TYPE OF CHROMOSOMAL ALTERATION THAT
OCCURRED IN CULTURED 5HF-1 CELLS IS ANEUPLOIDYJ A LARGS MET^CENTRIC
CHROMOSOME 0* UNDETERMINED ORIGIN MAS OBSERVED.
PAGE 14
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GREGORY, PAULA E., =>ATRICIA N. HQWASD-PEE3LES, RUDOLPH 3. ELLENDER, AMD BILLY
J. MARTIM. 1980. C-BANDING OF CHR3MQSDM5S FROM THREE ESTABLISHED MARINE
FISH CELL LINES. COP
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HARSH8ARGER, JOHN C,, 2LLIGT R. JAC05S3N, CHARLIE £. SMITH, AND JOHN A.
COUCH. 1980. HHMATO^OIETIC NEOPLASMS IN INVERTEBRATES AMD COLD-BLOODED
VERTEBRATES. IN: ADVANCES IN COMPARATIVE LEJKEMIA RESEARCH, 1979. DAVID S.
YOHN, BORIS A. LAPIN, AND JAM-S R. 5LAKESLEE, EDITORS,
ELSEVIER/NORTH-HOLLAND, NEW YORK, NY. »P. 223-225.
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HENDRICKS, JERRY 0., THEODORE R. MEYERS, DENNIS W. SHELT3N, AND RUSSELL 0.
SlNNHUStR. 1982. LIVER NEOPLA5IA ANC INDUCTION Dp HEMATIC MIXED FUNCTION
OXIOASE ENZYMES THE RAINBOW TROUT FOLLOWING DIETARY EXPOSURE TO
8ENZOCA)PYRENE (A3STRACT). ANNU. MEET. AM. ASSOC. CAMCER RES. PRCC. 23:58.
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HENDRICKS, J.D., J.H. WALES, R.Q. SINNHU3ER» J.S. NIXON, P.M. LQVELAND, .AND
R.A. SCANLAN. 1980. RAIN30W TROUT CSALMO GAIRDMERI) EM3RYOS: A SENSITIVE
ANIMAL MODEL FOR EXPERIMENTAL C4RCINQGENESIS. FED. PR3C. 39C14):3222-3229.
(ERL,G8 X359).
DIETARY EXPOSURES HAVE DEMONSTRATED RAINBOW TROUT TD BE THE MOST
SENSITIVE EXPERIMENTAL ANIMAL TO THE HEPAT1CARCIN03ENICITY OF
AFLATOXIN BCD CA~B). MORE RECENTLY THE DEVELOPMENT OF AN ALTERNATE
EXPOSURE METH3D HAS SHOWN TROUT TO 3E EVEN MORE SENSITIVE TO AFB<1>.
THIS METHOD INVOLVES THE SINGLE EXPOSURE OF = £RT.ILE RAIN30W TROUT EGGS
(EMBRYOS) TO A 0,5 PPM AQUEOUS SOLUTION OF APS(l) FOR 30 MINUTES,
RINSING THE EGGS IN WATER, AND ALLOWING HATCHING, SWIM-UP AND THE
ONSET DF FEEDING TO PROCEED AS USUAL, RESULTING FISH ARE FED A CONTROL
DIET FOR 1 YEAR AT WHICH TIME APPROXIMATELY TWO-THIRDS OF THE
POPULATION WILL HAVE DEVELOPED HFPATOCSLLULAR CARCINOMA OF THE LIVER,
VARIABLES, SUCH AS EMSRYD AGE OF GREATEST SENSITIVITY, CARCINOGEN
CONCENTRATION, AND LENGTH 0* EXPOSURE, HAVE BEEN DE=INED FOR AFB(i>
EXPOSURES. SENSITIVITY A<=B(1) INCREASED WITH INCREASING AGE OF THE
EMBRYO, THE MODEL SYSTEM SHOWED A DOSE-RESPONSE TO INCREASING
CARCINOGEN CONCENTRATION, AND ONE-HALF HOU2 EXPOSURES APPEASED TO BE
OPTIMUM FOR AFSC1). EXPERIMENTS WITH OTHER CARCINOGENS HAVE SHOWN THE
EMBRYO MODEL SYSTEM T3 BE SENSITIVE TO THE HEPAT3CARCINOSENICITY OF
AFBC1) METABOLITES ,
i STERIGNATOCYSTIN, VERSICGLORIN A, DIMETHYLMITRQSAMINE, AND N-METHYL-N"
-NITRO-N-NITROSOGUANIDINE. THE LATTER COMPOUND ALSO INITIATED
NEPHR03LASTCMAS OF THE KIDNEYS- THE RESULTS DEMONSTRATE THE POTENTIAL
FOR THE TROUT EMBRYO TO BE DEVELOPED INTO A CONVENIENT, ECONOMICAL,
AND SENSITIVE WHOLE ANIMAL MODEL SYSTEM FQ3 EXPERIMENTAL
CARCINUGENESIS.
HILLE3ERT, SUSAN A., 3.J, MARTIN, AND R.D. ELLENDER. 1980. EXPOSURE OF A
TELEOST CELL LIN~ TD SUSPECTED CARCINOGENS. J. MISS. ACAD. SCI. 25:71-75.
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KQENIG, CHRISTOPHER C., DANIEL C. A3EL, COURTNEY W. KLINGcNSMITH, AND MICHAEL
B. MADDO:K. 1932, USEFULNESS OF THE SELF-FERTILIZING CYPRINODONTIO FISH,
RIVULUS MARMORATUS AS AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING
CARCINOGE^IESIS, TERATOGENESIS AND MUTAGENESIS. EPA-600/3-82-073, U.S.
ENVIRONMEMTAL PROTECTION AGENCY, ENVIRONMENTAL RESEARCH LABORATORY, GULF
BREEZS, FL. 129P.
RIVULUS MARMQRATUS IS A NATURALLY SELF-PERTILIZING CYPRINQQONTID <=ISH
INHABITING MANGROVE MARSHES THROUGHOUT THE CARIBBEAN, AS A RESULT OF
INTERNAL SELF-FERTILIZATION THIS OVIPAROUS SPECIES IS COMPOSED OF A
NUMBER OF ISQGENIC, HOMOZYGDUS LINES (CLONES), SEVERAL OF WHICH HAVE
SEEN IDENTIFIED BY HISTOCQM?ATI3ILITY EXPERIMENTS AND MAINTAINED IN
LABORATORY CULTURE FDR OVER 30 YEARS, SIMPLIFIED CULTURE AND HANDLING
METHODS ARE GIVEN AND DATA ARE °RESENTED ON THE REPRODUCTION, GROWTH
ANO DEVELOPMENT OF RIVULUS UNDER LABORATORY CULTURE AS A PRELUDE TO
THE EVALUATION 0= ITS POTENTIAL AS A BIOASSAY ANIMAL, SEVERAL TYPES OF
3IQASSAYS WERE RUN AND EVALUATED USING RIVULUS: BEHAVIORAL,
CARCIN2GENlClTY,TtRATQGENlCITY» TOXICITY, AND MUTAGENICITY. ADVANTAGES
AND DISADVANTAGES OF USING RIVULUS FOR SUCH BICASSAYS ARE DISCUSSED.
BEHAVI3RALLY, RIVULUS IS CAPABLE OF DETECTING AND AVOIDING WATER
CONTAMINATED WITH H2S. THEY RESPOND (EC50 = 123.6 PP3 H2S) BY LEAPING
FROM THE WATER AND REMAINING EMERGENT FOR VARIOUS PERIODS OF TIME
WHILE RESPIRING CUTAMEQUSLY, HrPATOCELLULAR CARCINOMA AMONG OTHER
PATHOLOGIC CHANGES WERE DESERVED IN LIVERS OF RIVULUS A YEAR AFTER
EXPOSURE OF ADULTS AND LARVAE TO OIETHYLNITROSAMINE (45, 30, AND 15
PPM I,M WATER) FOR 5 WEEKS AND 12 WEEKS, RESPECTIVELY, NO PATHOLOGIC
CHANGES WERE FOUND IN EMBRYOS EXPOSED SIMILARLY. HIGH RATES OF VARIOUS
SKELETAL MALFORMATIONS RESULTED IN OFFSPRING OF ADULTS EXPOSED TO
DliUTYL PHTHALATE (DBP) AND 2,3,4,S-TETRACHLQRQPHENOL (TEC?) AT
CONCENTRATIONS 0= 20, 10 AND 5? (D3P - 0.740, 0.370, 0.135 MG/L? TECP
- 0.220, 0.110, 0.055 MG/L) OF THE LARVAL 96-HOUR LC50. NO
DOSE-RESPONSE RELATIONSHIPS 0? SKELETAL MALFORMATIONS WERE FOUND FOR
SIMILAR EXPOSURES TO P6NTACHLOROPHSNOL, 2,3,5-TRICHLDROPHENQL OR
3ROMQFDRM, HOWEVER, CHRONIC EXPOSURE OF DEVELOPING HATCHLINGS TO TECP
RESULTED IN FIN AND GILL EROSION AND CHR3NIC EXPOSURE TO 8RQMOFQRM
PRODUCED DORSAL FIN ABNORMALITIES. AS PART OF A MUTAGENESIS 8IQASSAY
14 ENZYME SYSTEMS REPRESENTING 28 LOCI WERE SCREENED FOR THE THREE
LABORATORY CLONES AND ONE WILD-CAUGHT CL3NE BUT NO ELECTROPHORSTIC
DIFFERENCES WERE FOUND. ATTEMPTS TO CULTURE RIVULUS CELLS FAILED.
ALSO, THE KA3YOTYPE OF RIVJLUS IS NOT SUITABLE FOR SHORT-TERM
CYCTQGENETIC ASSAYS SUCH AS THE SISTER CHR3MATIO EXCHANGE CSCE) ASSAY,
ALTERNATIVELY, HOWEVER, THE TOAD-ISH COPSANUS TAU) POSSESSES A
SUITABLE KARYDTYPE FOR SCE ANALYSIS AND WE HAVE 3EEN SUCCESSFUL IN
CULTURING T3ADFISH CELLS TO =GURTH PASSAGE. INCREASED RATES QF SCE
WERE OBTAINED WHEN TOAOFISH CELLS WERE EXPDSED IN VITRO TO THE MUTAGEN
ETHYL McTHANESULFONaTE BUT NOT 3ROMOFORM. ANOTHER SET OF EXPERIMENTS
IS PRESENTED WHICH INVOLVES CHARACTERIZATION OF THE NATURE OF THE
TOADFISH CYTOCHROME P450 SYSTEM.
PAGE 19
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LATOUCHE, Y. DAVIDt AND MICHAEL C. MIX. 1982. EFFECTS OF DCPURATIQN, SIZE
AND SEX ON TRACE XETAL LEVELS IN SAY MUSSELS. MA?. POLLUT. BULL.
13C1K27-29, CeRLtGB X412).
THE EFFECTS OF OE?URATION, SIZE AND SEX DM THE CONCENTRATIONS 0?= MN,
Nit CU, ZN AND CD WfRfc DETERMINED FOP GGNADAL AND SOMATIC TISSUES OF
MYTILUS EDULIS CR3M YAQUINA BAY, OREGON. AFTER DEPUTATION, MN LEVELS
DECREASED SIGNIFICANTLY IN BOTH TISSUES WHILE CU AND NI CONCENTRATIONS
INCREASED IN SOMATIC TISSUES. THE LATTER FINDING MAY HAVE SEEN
ASSOCIATED WITH A STRESS RESPONSE MANIctSTED BY THE APPEARANCE OF NEW
8YSSAL THREADS AND ATTACHMENT TO THE VESSEL, LAS>G = R, AND PRESUMABLY
QLQERt MUSSELS CONTAINED HIGHER CONCENTRATIONS OF NI, CU AND CD IN
THEIR SOMATIC TISSJES WHILE SMALLER ANIMALS HAD GREATER SOMATIC LEVELS
OF MN. THERE WERE NC SIGNIFICANT DIFFERENCES IM G3NAD CONCENTRATIONS
BETWEEN THE TWO SIZE GROUPS- MANGANESi AND ZN LEVELS WERE GREATER IN
THE G3NADS OF FEMALE MUSSELS AND CD WAS MORE CONCENTRATED IN THE
SOMATIC TISSUES OF FEMALES.
LATOUCHE, Y. DAVID, AND MICHAEL C, MIX, 1982. SEASONAL VARIATIONS OF
ARSENIC AND OTHER TRACE ELEMENTS IN BAY MUSSELS , ZINC CZN), AND CADMIUM (CD); NI, CU,
ZN, AND CD ARE EPA PRIORITY POLLUTANTS.
PAGE 20
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LATOUCHE, YV-S D. 1981, TRACE METAL LEVELS IN A POPULATION OF MYTILUS
EDULIS FROM Y4QUINA BAY, OREGON. PH.D. THESIS. OREGON STATE UNIVERSITY,
CDRVALLIS, OR. 135?. CERL.G9 X408).
THE PRESENT STUDY IS CONCERNED WITH THE M. EDULIS REPSOOUCTIVE CYCLE
AND ITS EFFECT ON SOFT TISSJE CONTENT OF SIX TRANSITION METALS THAT
ARE IMPORTANT IN BIOLOGICAL FUNCTION AND/OR AS POLLUTANTS IN ESTUARINE
ENVIRONMENTS. A MATURAL POPULATION WITH \ SUITA3LY LftRSE NUMBER OF
INDIVIDUALS FROM
-------
MARTIN, 3.J., R.D. ELL5NDER, S.A. HILLSBERT, AND M.M. GUESS. IN PRESS.
PRIMARY CELL CULTURES. FROM THE TPLEOST, CYPRINQDON VARIEGATUS: CULTURE
ESTABLISMENT AND APPLICATION IN CARCINOGEN EX?CSUR= STUDIES- IN:
PROCEEDINGS OF THE SYMPOSIUM DN THE USE CF SMALL FISH SPECIES IN
CARCINOGENICITY TESTING, BETHESDA, MARYLAND, DECEMBER 8-10, 1981. U.S.
NATIONAL CANCER INSTITUTE. <£RL,GB X26D).
METHODS WERE DEVELOPED TO MAINTAIN C. VARIEGATUS FRY SCEPTICALLY FOR
EXTENDED PERIODS. PRELIMINARY STUDIES INDICATED THAT UNDER OPTIMUM
CONDITIONS STERILE EMBRYOS DEVELOP NORMALLY FOR A SUFFICIENT TIME TO
FUNCTION AS CARCINQGFN/TERATOGEN ASSAY SYSTEMS. AN EMBRYO-PRIMARY CELL
CULTURE TECHNIQUE WAS DEVELOPED THAT INCORPORATES, IN A SINGLE SYSTEM,
CERTAIN! CHARACTERISTICS OF BOTH INTACT EMBRYOS AND PRIMARY CELL
CULTURES, ALLOWING SIMULTANEOUS OBSERVATION OF THE EFFECTS OF
CARCIN3GENS ON THE WHOLE ORGANISM AND PRIMARY CELL MDNQLAYERS. THE
EFFECTIVE USE OF THESE SYSTEMS PROVIDES THE OPPORTUNITY TO STUDY THE
EFFECTS OF CARCINOGENS ON TtLEOSTS AT THE CELLULAR AND ORGANISMIC
LEVEL.
MARTIN, 3.J. 1982- DEVELOPMENT OF * CARCINOGEN ASSAY SYSTEM ^UTILIZING
fcSTUARINE FISHES. 5PA-63Q/3-82-091, U.S. ENVIRONMENTAL PROTECTION AGENCY,
ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, FL. 5QP,
THE OBJECTIVE OF THIS PROJECT WAS THE DEVELOPMENT OF SYSTEMS TO ASSAY
THE EFFECTS 3F CHEMICAL CARCINOGENS ON MARINE TELEOSTS. IT WAS
DETERMINED THAT LC-50 FDR BENZINDINE WITH RESPECT TO CYPRINOOON
VARIEGATUS WAS CA. 64 PPM, WEEKLY CONTAMINATIONS OF 1 PPM BENZIDINE
CAUSED SOME INDIVIDUALS T1 DEVELOP PRQLIFERATIVE LIVER LESIONS,
EXPOSURE "F C. VARItGATUS EA3LY EMB3Y3S PRODUCED THE FOLLOWING
ANOMALIES AT CONCENTRATIONS OF 50 PPM AND *BOV£: TUBcO HEART SYNDROME
WITH DISTENDED PERICARDIA, PO-3P CIRCULATION, SP4RSE DISTRIBUTION OF
MELANOPHORES, INABILITY TO HATCH, ABNORMAL HEAD MORPHOLOGY, SC3LIOSIS,
AND FAINT R3C PIGMENTATION. CHRONIC EXPOSURE OF A CELL LINE FROM
ARCHOSARGUS PROBATOCE?HALUS TO BENZIDINE AND SENZQ(A)PYRENE PRODUCED
MUTAGENIC EFFECTS. TWO NOVEL TECHNIQUES WERE DEVELOPED TD STUDY THE
EFFECTS Or CARCINOGENS ON c. VARIEGATUS AT THE CELLULAR-LEVEL — AN
ASEPTIC EMoRYO TECHNIQUE AND AN EM8RYO-PRIHARY CELL CULTURE TECHNIQUE.
STANDARD IMMUNOLOGICAL TECHNIQUES WERE MINIATURIZED TO STUDY THE
IMMUNE SYSTEM OF C. VARIEGATUS. SERUM ELECTROPHORESIS DISCLOSED THAT
THE SERUM PROTEINS OF 3ENEZIOINE-EXPOSEO FISH DIFFERED FROM UNEXPQSED
CONTROLS.
MARTIN, B. J., AMD W. W. GREENWICH. 1931. BENZIDINE TQXICITY (ABSTRACT),
J. MISS. ACAD. SCI. 26(SUPPL.):127.
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MARTIN, 3.J. 1980, EFFECTS OF PETROLEUM COMPOUNDS DN 5STUARIN? FISHES.
EPA-600/3-80-019, U.S. ENVIRONMENTAL PROTECTION AGENCY, ENVIRONMENTAL
RESEARCH LABORATORY, GULF BSEcZE, FL. 31P,
EFFECTS OF TH= CARCINOGENIC POLYCYCLIC ARDMATIC HYDROCARBONS CPAH),
3ENZOCA)PYS£Nfc OAP), AND METHYLCHOLA'JTHRENE (MCA) WERE INVESTIGATED
WITH SHEEPSHEAD MINNOWS (CYPRINDDDN VARTEGATUS) AND CHANNEL CATFISH
CICTALUR'JS PUNCTATUS). A CLOSED-CIRCULATING SYSTEM WAS DESIGNED TO
MAINTAIN UP TO 100 SHEEPSHEAD MINNOWS IN ARTIFICIAL SHATTER FOR
LONG-TERM EXPOSURES. FISH WERE MAINTAINED IN THIS SYST=M FOR UP TO 31
WEEKS WITH WEEKLY CONTAMINATIONS OF PAH. DUE T3 THEIR CHEMICAL
PROPERTIES SIGNIFICANT LEVELS OF BAP AND MCA REMAINED IN THE WATER
COLUMN FDR ONLY CA. 24 HOURS EACH WEEK AND NO TUMORS WERE OBSERVED IN
THE EXPOSED FI5H DURING THE PERIOD 0" THE STUDY. THE INCIDENCE AND
TYPES OF LESIONS IN CONTROL AND EXPOSCD FISH WERE BASICALLY SIMILAR
EXCEPT IN CATFISH THAT WERE FED PAH CONTAMINATED FOOD, HIGH LEVELS OF
CONTAMINATION C1MG/3M FOOD) APPEARED TO BE TOXIC AND LOWER LEVELS OF
CONTAMINATION (0,1 MG/GM POOD) PRODUCED SUFFICIENT STRESS TO MAKE THE
CATFISH SUSCEPTI3LE TO FATAL PARASITE INFESTATIONS. BOTH SPECIES
ACCUMULATED RADIQACTIVELY LABELLED PAH AT CONCENTRATIONS MUCH HIGHER
THAN THEIR NDRMAL CONCENTRATIONS IN THE WATER. THESE ReSLTS
DEMONSTRATE THAT SHESPSHEAD MINNDWS FUNCTION WELL AS EXPERIMENTAL
ORGANISMS IN ARTIFICIAL SEAWATER IN A CLOSED SYSTEM MAINTAINED AT A
NONCOASTAL FACILITY. THUS, THEY PROVIDE AN EXCELLENT MODEL SYSTEM FOR
THE STUDY OF LOMG-TERM EFFECTS OF CHRONIC EXPOSURE TO POLLUTING
AGENTS.
MARTIN, B.J., AND W.W. GREENWICH. 1980. EXPOSURE OF TWO TELEOST SPECIES TO
POLYCYCLIC ARDMATIC HYDROCARBONS (ABSTRACT). J- MISS. ACAD- SCI.
XXVCSUPPL.):120. (ERL,GS X194*).
SHEEPSHEAD MINNOWS, CYPRINODDN VARIEGATUS, AND CHANNEL CATFISH,
ICTALURUS PU'4CTATUS, WERE MAINTAINED IN WATER CONTAMINATED WEEKLY WITH
POLYCYCLIC ARDMATIC HYDROCARBONS (PAH). IN OTHER EXPERIMENTS, FISH
WERE MAINTAINED ON PAH CONTAMINATED FOOD REGIMES. BECAUSE OF THE
SOLUBILITY PROPERTIES 3F PAH, SIGNIFICANT LEVELS DID NOT REMAIN IN THE
HATER COLUMN. HIGH LEVELS OF FOOD CONTAMINATION APPEARED TO STRESS
CATFISH SUFFICIENTLY TO INCREASE THEIR SUSCEPTIBILITY T3 PARASITIC
INFESTATION. EXPERIMENTS IN WHICH RADIQACTIVELY LABELLED PAH WAS
PLACED IN THE WATER COLUMN INDICATED A RATHER VARIABLE
8IOACCUMULATIQM, HIGHEST LEVELS 3F LABEL WERE OBSERVED IN GILL AND
LIVE* TISSUE, 4N INTERMEDIATE LEVEL IN GI TRACT, AND A LOWER LEVEL IN
SKELETAL MUSCLE. THE VARIABILITY OF THESE DATA WAS REDUCED WHEN FISH
WERE FED LA3ELL-D PAH. TH'E RESULTS SUSGrST THAT SIGNIFICANT EXPOSURE
OF FISH TO PAH IS MORE LIKELY TC OCCUR AS A RESULT OF THEI3 INGESTING
CONTAMINATED MATERIAL, THAN FROM DIRECT BDDY EXPOSURE TO PAH IN THE
WATER COLUMN.
PAGE 23
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McADOR, C.3., B.L. MIDQLEBRQQK3, AMD 3.J. MARTIN, IN PRESS. SEROLOGIC
ALTERATIONS IN CARCINOGEN-EXPOSED TELEQSTS: PROCEDURES FOR PREPARATION AND
ANALYSIS Or SAMPLES *=ROM SMALL FISH. IN: PROCEEDINGS OF THE SYMPOSIUM ON
THE USE 0= SMALL SISH SPECIES I E?'JE QUINONES BY POST MI TOCHONDRIAL SUPERNATANT 3R
MICROSQMES IM SUCH FISH AS THE RAINBOW TROUT FLOUNDER, SALMON, MULLET,
LITTLE SKATE, FUNDULUS GRANOIS AND SEA CATFISH. IT IS ALSO NOW
WELL-ESTABLISHED THAT MANY FISH CONVERT 3ENZOCA)PYRENS TO POTENT
MUTAGENIC MTEBOLITES AS HAS 3ESN 05MHN3TATED USING THE AMES TEST,
ESPECIALLY WHEN THE =ISH A3E INDUCED WITH AROCLOR OR
3-METHYLCHOLANTHSEME, THE METABOLITE PATTERNS 03TAINEO AT DIFFERENT
SUBSTRATE CONCENTRATION LEVELS INDICATE THAT THE METABOLISM is MORE
COMPLEX AT LDW CONCENTRATIONS WHERE RECYCLING OF METABOLITES is
PRODUCED IN THE IN VITRO SYSTEM.
PAGE
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HELIUSt P., 0. ELAM, M. KILGORE, AND W.P. SCHOOR. 1979- INDUCTION OF
PQLYAROMATIC HYORQCAR3CN DXIDASS IN MARINE ORGANISMS (ABSTRACT). IN:
ABSTRACTS: XITH INTERNATIONAL CONGRESS o<= aiocnEMiSTRY, JULY 3-13, 1979,
TORONTO, CANADA. NATIONAL RESEARCH COUNCIL 0= CANADA. TORONTO. P°. 691.
(ERL.G3 X277*).
CERTAIN KINDS OF DRUGS, SUCH AS PHENOBAR3ITAL, AND ENVIRONMENTAL
POLLUTANTS, SUCH AS POLYHALDGENAT£D BIPHSNYLS, ARE WELL-KNOWN TO
INDUCE CYTOCHROME P450 ENZYMES IN RAT LIVER. THE CYTOCHRCME P450
ENZYME SYSTEMS ARE RESPONSIBLE FOR SUCH CONVERSIONS AS BENZOCA)PYRENE
(BP) TO A MUTAGENIC AND CARCINOGENIC BENZO(A)PYRENE EPOXIDE. OUR
REPORT IS CONCERNED *)ITH THE TREATMENT OF MULLcT (MUGIL CEPHALUS) AND
KILLIFISH (FUNDALUS GRANDIS) WITH AROCLOR 1254 AT LEVELS FROM 25 TO
200 MG/K. THE INCREASE IN MIXED FUNCTION OXIOASES HAS BEEN MEASURED 3Y
USING THE AMES TEST ON MTCROSOMAL FRACTIONS PREPARED FROM THE FISH
LIVERS WITH 3ENZO(A)PYRENE, 3-MSTHYLCHOLANTHRENE AND CRYSENE AS THE
SUBSTRATES. W? HAVt ALSO MEASURED THE 3P DXIDASE, THE HEPATIC
MICROS3MAL RcDUCTASES AND CYTQCH^OME P450 ACTIVITIES IN THE LIVER
MICROS3MAL PREPARATIONS- THE AROCLOR INDUCED LIVERS WERE FQUNO TO BE
CAPABLE OF ACTIVATING CERTAIN COMPOUNDS TO MUTAGENS.
MELIUS, PAUL, DAVID ELAM, MELVIN , META30LITIES THAN DID 3-MC-TREAT5D GULF KILLIFISH.
THIS MAY HAVE RESULTED BECAUSE (1) BAP IS A LESS EFFECTIVE INDUCER
THAT 30-MC OR (2) IN VIVO 3AP METABOLITE MAY HAVE INDUCED THE
CONJUGATION SYSTEMS, WHICH WOULD RESULT IN ETHYL ACETATE INSOLUBLE AND
NONMUTAGENIC IN VITRO BAP METABOLITES. THESE STUDIES SHOW THAT CERTAIN
SIMILARITIES EXIST IN THE MECHANICS OF FISH &NO MAMMALIAN MFO SYSTEMS
EVEN THOUGH DEFERENCES EXIST IN THE ACTIVITIES OF THESE' SYSTEMS.
CHAMBERS AND VARBROUGH SPECULATE THAT THE LESS ACTIVE MFO SYSTEMS OF
FISH REFLECT THEIR EVOLUTIONARY ADAPTATION TO AN ENVIRONMENT IN WHICH
XEN03IOTICS ARE NATURALLY DILUTED.
PAGE 25
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MEYERS, THEODORE R.f AND JERRY D. H5NORICKS. 1984. LIMITED EPIZOOTIC OF
NEUROBLASTOMA IN COHD SALMON REARED IN CHLQ2IN4T£D-0CCHLORINATED WATER. J.
HAT. CANCER INST. 72C2):299-310. CERL,G3 X458*>.
DURING THE 1976-77 SROOD YEAR, APPROXIMATELY 12 CASES OF NEUR03LASTQMA
WERE OBSERVED IN A CAPTIVE GROUP Of 100,003 =ING:RLING COHO SALMON
(ONCQRHYMC4US KISUTCH) REARED IN A COMMERCIAL HATCHERY. THE TUMORS
WERE LA^GE, OCCURRING I»M THE SKELETAL KJSCLE NEAR THE DORSAL FIN
CAUSING CONSPICUOUS BULGING 0= THE .OVERLYING INTEGUMENT. TUMORS
EXAMINED 'RDM 3 FISH EACH CONSISTED OF NEUR03LASTS IN TRA5ECULAR
PATTERNS INJTERSPE3SED BY GLIAL FIBRILLAR MATERIAL AND LINEAR CAVITIES
RESEMBLING CENTRAL NEURAL CANALS LINED BY EPENOYMA-LIKE CELLS.
GANGLION-LIKE CELLS ALSO WERE APPARENT MORPHOLOGICALLY AND BY SPECIAL
STAIN, CANCER OF THE TUMOR WAS CHARACTERIZED BY AM ABUNDANCE OF
MITOTIC FIGURES WITH OCCASIONAL ABNORMAL DIVISIONS, LOCAL INVASION OF
NQSMAL TISSUES, AMD POTENTIALLY METASTATIC TUMOR CELL AGGREGATES IN
ORGAN VASCULATURS. THE ETIOLOGY OF THIS TUMOR MAY HAVE BEEN RELATED TO
MUTAGENIC-CARCINDGcNIC HALOGENATED COMPOUNDS POSSIBLE FORMED IN THE
HATCHERY WATER SUPPLY DURING CONTIGUOUS CHLQRINATION OF INCOMING RIVER
WATER
MEYERSt T.R., AND J.C. HENDRICKS. 19^3. HISTOPATHOLOGY OF FOUR SPONTANEOUS
NEOPLASMS IN THR5S SPECIES C= SALMONID FISHES. J. FISH DIS. 6C5):481-499.
(ERL.GB X358).
GROSS AND -USTOLQGICAL DESCRIPTIONS OF "OUR DIFFERENT SPONTANEOUS
NEOPLASMS I'-l THREE SPECIES OF SALMONID FISHES ARE PROVIDED: THYMIC
LYMPHC^IA AND DERMAL FIBRGSARCOMA, RESPECTIVELY, IN TWO ARTIFICIALLY
REARED SDCKEYE SALMON, ONCORHYNCHUS NERKA CWALBAUM), RENAL
PAPILLIFER3US CYSTiDENQMA IN A WILD CAUGHT CHINOOK SALMON, 0.
TSHAWYTSCHi C*AL8AUM)J CAPILLARY HAEMAN3IOMA OF THE DERMIS IN AN
ARTIFICIALLY SEARED RAINBOW TROUT, SALMD GAIRDNERI RICHARDSON. THE
FREQUENCY OF OCCURRENCE OF THESE AND RELATED TUMOUR TYPES, AS REPORTED
IN THE LITERATURE, A^E COMPARES IN SALMONID AND NON-SALMONID FISH.
MEYERS, THEODORE R., AND JER5Y 3. HENDRICKS, 1932. SUMMARY OF TISSUE
LESIONS IN AQUATIC ANIMALS INDUCED BY CONTROLLED EXPOSURES TO ENVIRONMENTAL
CONTAMINANTS, CH6MOTHERAPEUTIC AGENTS, AND POTENTIAL CARCINOGENS. MAR.
FISHERIES REV. 44Ci2):i-i7. CERL.GB X368).
THIS ARTICLE WAS WRITTEN TO PROVIDE A USEFUL REFERENCE FOR AQUATIC
TOXICDLOGISTS/^ATHOLGGISTS BY CONSOLIDATING THIS DESCRIPTIVE MATERIAL
WITH' KEY R-FSRENCPS THROUGH 1981. THIS INFORMATION IS PRESENTED IN
FIVE TABLES. T4BLE 1 IDENTIFIES AT LEAST 90 ENVIRONMENTAL CONTAMINANTS
USED AS PESTICIDES, HERBICIDES, OR AS INDUSTRIAL CHEMICALS WHICH ARE
CATEGORIZED IN THE FOLLOWING MANNER: QRGAMOCHLORINES, PirTROLEUM
COMPOUNDS, 03GANJ-PHQSPHATES, CARBAMATESt HEAVY METAL SALTS,
NITROGENOUS COMPOUNDS, .MISCELLANEOUS COMPOUNDS, AND CHEM3THERAPEUTIC
AGENTS. TABLE 4 LISTS AT LEAST 50 COMPOUNDS GROUPED AS MYCQTOXINS,
PLANT DERIVATIVES, AND OT.HE* CHEMICALS ALL OF WHICH HAVE BEEN TESTED
FOR CARCINOGENIC aCTIVITY. TABLES 2, 3 ANO 5 DESCRIBE AND REFERENCE
HISTOLOGIC ANO/OR ULTRASTRUCTURAL LESIONS OR ABNORMALITIES CAUSED BY
THESE COMPOUNDS, THEIR ROUTES OF EXPOSURE, AND AQUATIC SPECIES TESTED.
26
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MIX, MICHAEL C,, AND SANDY L. SCHAFPEPi. 1933. CONCENTRATIONS OF
UNSUBSTIT'JTfC POLYCYCLIC AROMATIC HYOROCAR3DNS IV SO-TSHELL CLAMS FROM COOS
BAY, 3REG3N, USA. MAR. P3LLUT. BULL. 14C3K34-97. C£RL,G3 X338).
CONCENTRATIONS OF BENZCCA)PYRENE (3AP) WERE MEASURED IN SU3POPULATIONS
3F SOFTSHEL;. CLAMS, MYA ARENA*!*, FROM FOUR IMTERTIOAL SITES IN coos
3AY FROM JUNE 1976 TO JUNE 1978. SUBSEQUENTLY, CONCENTRATIONS OF 15
UNSUBSTITUTED PQLYNUCLEAR AR3MATIC HYDROCARB3NS
-------
MlXt MICHAEL t. 1983. STUDIES ON POLYNUCLEAR AROMATIC HYDROCARBONS AND
METALS IN MOLLUSCS (ABSTRACT), IN: WORKSHOP CM SU8LETHAL EFFECTS OF STRESS
ON MARINE ORGANISMS, MARCH 30-31, 198Z, ASILOMAR, PACIFIC GROVE, CALIFORNIA.
MICHAEL MARTIN AMD FLORENCE HARRISON, EDITORS, NTIS, SPRINGFIELD. VA. PP.
55. CERLtG3 X233*).
DURING THE PAST 5 YEARS, OUR R=S^ARCH HAS -OCUSED ON MEASURING LEVELS
OF 15 UNSU3STITUTEO PCLYNJCLEAR AROMATIC HYDROCARBONS CPNAH) AND
CERTAIN METALS IN BIVALVE MOLLUSCS = ROM INDIGENOUS POPULATIONS' THAT
INHABIT OREGON ESTUARIrS. THE PRIMARY OBJECTIVES OF SOME OF THE MAJOR
STUDIES WERE TO: CD MEASURE CONCENTRATIONS Or PNAH AND METALS IN
SHELL FISH F*OM DIFFERENT LOCATIONS =0tj AN EXTENDED PERIOD OF TIME IN
ORDER TO ESTABLISH A DEPENDABLE BASELINE, C2) DETERMINE WH-THER OR NOT
PNAH CONCENTRATIONS IN MOLLUSCS REFLECT THE DEGREE OF ENVIRONMENTAL
CONTAMINATION, (3) EVALUATE SEASONAL VARIATIONS IN PNAH AND METAL
CONCENTRATIONS IN MOLLUSCS, C4) DETERMINE WHETHER OR NOT THERE WERE
STATISTICAL CORRELATIONS BETWEEN THE PRESENCE OF CELLULAR
PROLIFERATES DISORDERS IN SUBPQPULATIONS OF MUSSELS
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MIX, MICHAEL C. 1982. P3LYNUCLEAR AROMATIC HYDROCARBONS AND CELLULAR
PROLIFERATXVE DISORDERS IN BIVALVE MOLLUSCS FROM OREGON ESTUARIES.
EPA-600/4-82-Q26, U.S. ENVIRONMENTAL PROTECTION AGENCY, ENVIRONMENTAL
RESEARCH LABORATORY, GULF 6REEZ?, FL. 49?,
THE RESEARCH PROJECT INVUVED UTILIZING INDIGENOUS POPULATIONS OF
ECONOMICALLY-IMPORTANT BIVALVE MOLLUSCS AS MONITORS FOR DETECTING AND
QUANTISING ENVIRONMENTAL PNAH, INCLUDING 11 COMPOUNDS CLASSIFIED AS
CARCINOGENS, 11 EPA PRIORITY POLLUTANTS AND 11 TOXIC POLLUTANTS.
BASELINE LEVELS OF PNAH WERE DETERMINED DJRING A TWO-YEAR PERIOD FOR
MUSSELS CM. EDULIS), CLAMS CM. ARENARIA AN3 T. CAPAX) AND OYSTERS CC.
GIGAS) FROM DIFFERENT SITES, RANGING FROM RELATIVELY PRISTINE TO
MODERATELY POLLUTED, IN YA3UINA, COOS AND TILLAMQOK BAYS, OREGON,
TOTAL CONCENTRATIONS 0= 15 UNSU3STITUT5D PNAH WERE 30-60 UG/KG IN
SHELLFISH FROM UNC3NTAMINATED WATERS TO GREATER THAN 1000 UG/KG IN
THOSE FROM SITES CLASSIFIED AS CONTAMINATED. A MAJOR EFFORT WAS MADE
TO DETERMINE AMD EVALUATE CERTAIN RELATIONSHIPS BETWEEN PNAH AND THEIR
CONCENTRATIOMS IN SHELLFISH. STUDIES WERE CONDUCTED TO: DETERMINE THE
EFFECTS OF DEPURATION ON PNAH CONCENTRATIONS; IDENTIFY SEASONAL
DIFFERENCES IN PMAH CONCENTRATIONS; AND MEASURE BAP UPTAKE AND
ELIMINATION. PRELIMINARY STUDIES INDICATED THAT MUSSELS MAY POSSESS A
LIMITED ABILITY T3 METABOLIZE 3AP. MULTIPLE REGRESSION AND MULTIPLE
CORRELATION TECHNIQUES WERE USED TO IDENTIFY AND EVALUATE
INTERRELATIONSHIPS BETWEEN ?NAH. CERTAIN RELATIONSHIPS MAY 3E USEFUL
FOR PREDICTIVE PURPOSES IN EVALUATING ENVIRONMENTAL PNAH. THE DATA
FROM THESE STUDIES INDICATE THAT IT MAY BE POSSIBLE TO IDENTIFY
SITE-SPECI'IC, SIGNIFICANT VARIABLES (INDIVIDUAL PNAH) AFTER A
SUITABLE PERIOD OF SAMPLING AND TO SUBSEQUENTLY MEASURE ONLY THOSE KEY
VARIABLES F3R AN ADEQUATE ASSESSMENT Or TOTAL PNAH. COMBINED WITH
OTHER APPROACHES, THIS MAY RESULT IN CONSIDERABLE COST REDUCTIONS FOR
LONG-TERM BIOLOGICAL MONITORING PROGRAMS. CELLULAR PROLIFERATIVE'
DISORDERS, RESEMBLING NEOPLASTIC CONDITIONS IN VERTEBRATES, WERE FOUND
IN MUSSELS WIT-I THE GREATEST PNAH CONCENTRATIONS. CURTHER STUDIES WILL
BE NE:ESSARY TO DETERMINE THE SIGNIFICANCE OF THIS CORRELATION. THIS
REPORT WAS SUBMITTED IN FULFILLMENT OF CONTRACT NO. R806224020 8Y
OREGON STATE UNIVERSITY, CDRVALLIS, OREGON, UNDER THE SS>ONSORSHIP OF
THE U.S. ENVIRONMENTAL PROTECTION AGENCY. THIS REPORT COVERS THE
PERIOD OCTOBER 1, 1978 TO NOVEMBER 30, 1980.
MIX, MICHAEL C., RANDY L. SCHAFFER* AND SUSAN J. HEMINGWAY. 1981.
POLYNUCLEAR AROMATIC HYDROCARBONS IN BIVALVE MCJLLUSKS IN 3&Y MUSSELS CMYTILUS
EDULIS) FROM OREGON. IN: PHYLETIC APPROACHES TO CANCER: PROCEEDINGS OF THE
11TH INTERNATIONAL SYMPOSIUM OF THE PRINCESS TAKAMATSU CANCER RESEARCH FUND,
TOKYO, 1980. CLYDE J. D4WE, EDITOR, JAPAN SCI. SOC. PRESS, TOKYO. PP.
167-177. (ERL,G3 X355).
TOTAL CONCENTRATIONS OF 15 UNS'JBSTITUTED P3LYNUCLEAR AROMATIC
HYDROCARBONS
-------
MIX, MICHAEL C., AND WILBUR P. 3REESE. 1980. CELLULAR PRQLIFERATIVE
DISORDER IN OYSTERS OSTREA CHILENSIS) FR3M CHILOr, CHILE, SOUTH AMERICA. J.
INVERTE3R. PATHOL. 36C1):123-124. CERL,GB X252*),
BEGINNING IN THE LATE 1950S AND THRCUGH-OUT THE PAST DECADc, UNUSUAL,
PERHAPS NEOPLASTIC, LARGE CELLS WITH RATHER CONSISTENT CHARACTERISTICS
WERE DESCRIBED IN MUSSELS, CLAM, AND OYSTERS FROM SEVERAL BAYS IN THE
UNITED STATES AND THE JNITcD KINGDOM
-------
MIX, MICHAEL C., STEVE R. TRENHDLM, AND KEITH I. KING. 1979. BENZQCA)PYRENE
BODY BURDENS AND THE PREVALENCE OF PROLIFERfiTlVE DISORDERS IN MUSSELS
(HYTILUS EDULIS) IN OREGON. IN: ANIMALS AS MONITORS OF ENVIRONMENTAL
POLLUTANTS. NATI3NAL ACADEMY OF SCIENCES, WASHINGTON, DC. PP. 52-62.
<£RL,GB XL454O.
LEVELS OF BENZ3EA3PY3ENE (3P> IN ECONOMICALLY IMPORTANT SHELLFISH <=ROM
OREGON ESTUARIES WERE STUDIES. SHELLFISH FROM THE MORE POLLUTED AREAS
OF YAQUINA EAY, DREG. CSITES Y2M, Y4M, FIGURE 1), CONTAINED
SIGNIFICANT LEVELS OF BP CLESS THAN 15 UG/KG>, WHILE THOSE FROM
CLEANER AREAS CSITES Y1M, Y12M, FIGURE 1) CONTAINED VERY LOW OR
UNDETECTABLE LEVELS OF BP. HISTQLQGICAL STUDIES HAVE SHOWN THAT NEARLY
10% OF TH£ MUSSELS WITH SIGNIFICANT 3P SIDY 3UROEMS HAVE APPARENT
PRQLIFERATIVE DISORDERS COMPARED TO NONE F3R TrlOSE SAMPLED FROM CLEAN
SITES.
MIX, MICHAEL C, , AND SANDY L. SCHAFFER. 1979. SENZQCA)PYREN£ CONCENTRATIONS
IN MUSSELS CMYTILUS EDULIS) FROM YAQUINA BAY, OREGON DURING JUNE 1976 - MAY
1978. BULL. ENVIRON. CONTAM. TOXICOL. 23C4/5):677-684. (ERL,GB X148*).
B£NZOCA)PYRENE C3AP) AND SEVERAL OTHER POLYNUCLEAR AROMATIC
HYDROCARBONS CPNAH), MANY WHICH ARE CARCINOGENIC IN VERTEBRATES, ARE
FOUND IN L3W LEVELS IN CRUDE OIL, PARTICULARLY REFINED OILS, AND
THOUSANDS OF KG 3F THESE COMPOUNDS ENTER THE SEA EACH YEAR. WEATHERED
AND PARTIALLY DEGRADED OILS MAY ALSO CONTAIN ADDITIONAL OXIDATION
PRODUCTS OF POTENTIAL CARCINOGENICITY. MORE RECENTLY, SEVERAL REPORTS
INDICATE THAT, IN GENERAL, PNAHS FOUND AT PP3 LEVELS IN MARINE ANIMAL
TISSUES ARE DERIVED FROM COMMON COMBUSTION SOURCES ANS NOT DIRECTLY
FROM PETROLEUM CONTAMINATION, THE USE OF BIVALVE MOLLUSKS FOR
MONITORING MARINE ENVIRONMENTS IN ORDER TO DETECT AMD QUANTITATE
VARIOUS POLLUTANTS, INCLUDING CHEMICAL CARCINOGENS, HAS BEEN ADVOCATED
BY MANY INVESTIGATORS. INDIGENOUS POPULATIONS 3F SHELLFISH SEEM TO BE
IDEAL SUBJECTS ?Q* EVALUATING CARCINOGENIC PNAH LOADS IN THE MARINE
ENVIRONMENT, THE PURPOSES OF THIS STUDY WERE TO + MEASURE BAP
CONCENTRATIONS IN INDIGENOUS POPULATIONS OF MUSSELS FOR A TWO-YEAR
PERIOD, DETERMINE SEASONAL FLUCTUATIONS IN 3AP BODY BURDENS, AND
ANALYZE =ACTORS THAT MAY INFLUENCE TEMPORAL CONCENTRATION PATTERNS-
PAGE 31
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MICHAEL C. 1979. CHEMICAL CARCINOGENS IN BIVALVE MOLLUSKS FROM OREGON
ESTUARlcS. EPA-6QO/3-79-Q34, U.S. ENVIRONMENTAL PROTECTION A5ENCY,
ENVIRONMtNTftL RESEARCH LABORATORY, GULF BREEZE, FL. 33P,
THE RESEARCH UNDERTAKEN INVOLVED THE USE Oc INDIGENOUS POPULATIONS 'OF
BIVALVE MOLLUSKS AS MONITORS FOR DETECTING AND QUANTIFYING
ENVIRONMENTAL 3ENZO<4>PY*ENE (SAP) IN OREGDN ESTUARIES, SHORT-TERM AND
LONG-TERM STUDIES WERE CONDUCTED IN ORDER TO ESTABLISH BASELINE LEVELS
OF BA? AND TO IDENTIFY SEASONAL VARIATIONS IN BAP CONCENTRATIONS IN
SHELLFISH. A PRESUMPTIVE CELLULAR PROLI=EQATIVE DISORDER, THOUGHT
POSSIBLY TO 3E VEOPLASTIC. WAS ALSO STUDIED IN MUSSELS, MYTILUS
EDULIS, FROM YAQUINA 3AY. HlSTQLQGlCAL STUDIES REVEALED THAT MUSSELS
INHABITING POLLUTED ENVIRONMENTS, AND WITH HIGH 3AP 500Y BURDENS, HAD
AN AVERAGE 6-82 PREVALENCE OF THE CELLULAR PROLIFERATIVE DISORDER
WHILE THOSE FROM CLEAN ENVIRONMENTS AND WITH LOW OR UNDETECTA8E
LEVELSt DID NOT HAVE THE DISORDER. THE CELLULAR CONDITION SHOWED A
DEFINITE SEASONAL ?ATTERN» THERE WAS A LOW PREVALENCE DURING THE
SUMMER AND FALL FOLLOWED BY AN INCREASE DU3ING THE EARLY WINTER AND A
PEAK PREVALENCE OCCURRED IN JANUARY-FEBRUARY, THE ATYPICAL, LARGE
CELLS THAT CHARACTERIZE THE DISORDER IV M. EDULIS POSSESS MANY
ULTRASTRUCTURAL PROPERTIES IN COMMON WITH MALIGNANT VERTEBRATE CELLS.
FURTHER STUDIES ARE REQUIRED TO EVALUATE THE PUBLIC HEALTH
SIGNIFICANCE OF THESE RESULTS.
MIX, MICHAEL C., JOYCE W. HAWKES, AND ALBERT K. SPARKS. 1979. OBSERVATIONS
ON THE ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTATIVE NEOPLASTIC
DISORDERS OF MUSSELS, MYTILUS EDULIS, FROM YAQUINA BAY, OREGON. J.
INVERTE3R. PATHOL. 3U 1) : 41-56.
-------
MJXf MICHAEL C., DIANE L. 3UNTING, AND C.T. A333TT. 1979, PRFLIMINARY
STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARVAL STAGES OF THE
GOOSE BARNACLE, POLLICIPES PQLYMERUS FOR MARINE SIQASSAYS. IN: PROCEEDINGS
OF THE SECOND BIENNIAL CRUSTACEAN HEALTH WORKSHOP, TAMU-SG-79-114. O.H.
LEWIS AND J.K. LEONG, EDITORS, TEXAS A £ M UNIVERSITY, COLLEGE STATION, TX.
PP. 361-331. (ERL,GB X166*}.
THc GENERAL PURPOSE OF THESE STUDIES WAS TO DETERMINE IF THE
DEVELOPMENTAL AND LARVAL STAGES OF THE 3GOSE BARNACLE, POLLICIPES
PQLYMERUS WOULD 3E USEFUL ORGANISMS TO JSE AS A MARINE 3IOASSAY
SYSTEM. DIFFERENT CULTURE SYSTEMS AND CULTURING CONDITIONS WERE
DEVELOPED AND EVALUATED. WE DESIGNED A SIMPLE, INEXPENSIVE SYSTEM THAT
CAN BE USED TO CULTURE FERTILE EGGS UNDER MDRMAL LABORATORY CONDITIONS
CRDOM TEMPERATURE ANC AMBIENT LIGHT-DARK CYCLE). THE CULTURE MEDIUM
CONSISTS OF INSTANT OCEAN SEA SALTS MIXED IN TAP WATER TO A SALINITY
OF 28-30 PARTS PER THOUSAND. ANTIBIOTIC CONCENTRATIONS OF 10 MG/L
SEAWATER OF STREPTOMYCIN SULFATE AND 10 rtG.L SEAWATER 3F PENICILLIN G
ARi TrtEN ADDED TO THE ARTIFICIAL SEAWATER. AS A RESULT OF THESE AND
OTHER STUDIES, Iy SEEMS LIKELY THAT 5. P3LYMERUS MAY BE A VALUABLE
8IOASSAY ORGANISM. MG?.E COMPLETF STUDIES, NECESSARY TO GATHER LARGE
AMOUNTS OF DATA «QR DEVELOPING AND STATISTICALLY EVALUATING DIFFERENT
CRITERIA TD DETERMINE DELETERIOUS EFFECTS, ARE REQUIRED BEFORE A FINAL
JUDGEMENT CAN 3£ MAD = ABOUT THE «=UTURE USE OF P. POLYMERUS FOR
BIQASSAYS.
MIX, MICHAEL C., RCMALD T. RILEY, KEITH I. XING, STEVEN R. TRENHOLM, AND
RANDY L. SCHAFFER. 1977, CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT.
BENZOCA>PYRENE IN ECONOMIC ALLY-IMPORT ANT 3IVALVE MOLLUSKS FROM OREGON
ESTUARIES. IN: FATE AMD EFFECTS OF PETR-OLEUM HYDROCARBONS IN MARINE
ORGANISMS ANO ECOSYSTEMS. DOUGLAS A. WOLFE, EDITOR, ^ERGAMON PRESS, NEW
YORK, NY. PP. 4-21-431.
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MORENO, MARK, AND 3.J. MARTIN. 1979. EM3RYOLOGIC DEVELOPMENT OF THE
SHEEPSHEAD MINNOW CCYPRINOOON VARI6GATUS) (A3STRACT). J, MISS. ACAQ. SCI.
XXIV:(SUPPLEMENT116, (£RL,G8 X176).
SEXUALLY MATURE SPECIMENS COLLECTED FRQ'1 THE MISSISSIPPI GUL = COAST
WERE MAINTAINED IN AQUARIA AT 15-20% SALINITY AND 30 DEGRF^S CELSIUS.
THE PHQTOPERIDD VlAS MAINTAINED AT 14 HOU3S LIGHT AND 10 HOURS DARK
THROUGHOUT THE EXPERIMENT. E3GS WERE OBTAINED BY TEMPERATURE INDUCED,
OR HUM*N CH03IONIC GON4DCTRQPIN INDUCED SPAWNING. DATA INCLUDE
MECHANISMS OF: FERTILIZATION, BLASTULATI IN, G4STRULATION, EPIBOLY,
AND ORGAN3GENESIS. THIS STJDY SUGGESTS A POSSIBLE SIGNIFICANCE OF
CERTAIN "WcDG£-LIKE» CELLULAR STRUCTURES SEEN IN EARLY CLEAVAGE.
PATTON, JOHN S.» AND JOHN A. COUCH, IN PSESS. CAN TISSUE ANOMALIES THAT
OCCUR IN MA3IME FISH IMPLICATE SPECIFIC POLLUTANT CHEMICALS?. INt
PROCEEDINGS Oc CONFERENCE ON MEANINGFUL MEASURES OF MARINE POLLUTION EFFECTS,
APRIL 25-29, 1932, ?ENSACOLA 3EACH, FL. U.S. NATIONAL OCEANIC AND
ATMOSPHERIC ADMINISTRATION. (ERL.GB 474).
THE ADVANTAGE 3F USING TISSUE ABNORMALITIES IN WILD FISH AS A MEASURE
OF FISH HEALTH IS THAT .THE ABNORMALITY, UMLIKE SENSITIVE BIOCHEMICAL
ANOMALIES, CANMOT 3E SAID TO HAVE OCCURRED DURING CAPTURE 3R TRANSPORT
TO THE LA3. IT USUALLY TAKES HOURS, DAYS, WEEKS, AND SOMETIMES EVEN
MONTHS FOR A3N3RMAL TISSUE PATHOLOGIES TO DEVELOP. THE RESEARCHER CAN
BE CONFIDENT THAT SOME FACTOR IN THE FIELD CAUSED THE ABORMALITY. WHEN
AN ABNORMAL ^=ISH IS CAPTURED, LOGICAL QUESTIONS APPEAR AT FOUR
DIFFERENT LEVELS: 1) WHAT IS THE STRUCTURE OF MORPHOLOGY OF THE
ABNORMALITY? M4NY SCHOLARLY ARTICLES 3Y HISTDP.ATHOLOGI STS DESCRIBE IN
DETAIL TISSUE ANOMALIES OBSERVED IN CIELD SPECIMENS. AT THIS LEVEL THE
FOCUS IS ON THE PATHOLOGY ITSELF. 2) WHAT IS THE INCIDENCE OF THE
PATHOLOGY IM THE POPJLATION? HOW MANY ARE AFFLICTED, OLD OR YOUNG,
MALE OR FcMALr? HERE THE FOCUS IS ON THE SPECIES POPULATION. 3> CAN
THE INCIDENCE 3? A FISH DISEASE 3E LINKED TO ENVIRONMENTAL POLLUTION?
HERE THE FOCUS IS ON CCS2-LATING PATHOLOGY WITH POLLUTION. 4) WHAT
FACTOR OR FACTORS (CHEMICAL, PHYSICAL, AND/OR SIQLOGICAL) CAUSED THE
PATHDLOGY IN THE P1LLUTED WATERS? THIS IS PROBABLY THE MOST DIFFICULT
QUESTION TO ANSWER AND THE SUBJECT 0= THIS PAPER. ANSWERS TO THE FIRST
THREE QUESTIONS MUST BE FOUND BEFORE ATTEMPTS CAN 3E MADE AT ANSWERING
THE F3URTH. IF A SPECIFIC FISH DISEASE C&N BE LINKED TO A SPECIFIC
XENOBI3TIC, THEN A ANOTHER TIER 0= QUESTI3NS ARISES. 5) WHAT IS THE
SIGNIFICANCE 0= THIS TO HUMAN HEALTH AND WELL 3EING? IS A FOOD SOURCE
DIMINISHED, ARE HUMANS INGESTING FISH CONTAINING T3XIC CHEMICALS? WHAT
IS THE AESTHETIC/ECONOMIC COST VERSUS THE INDUSTRIAL/ECONOMIC GAIN OF
HAVING CONTINUED P3LLUTIDN? ANSWERS TO THESE QUESTIONS ARE BEYOND THE
SCOPE 3F THIS PAPER. THIS PAPER WILL FOCUS ON THE QUESTION - ARE THERE
POLLUTANT SPECIFIC PATHOLOGIES IN MARINE FISH?
"AGE 34
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FORTERf RONALD C., AND 3.J. MARTIN, 1980. HISTOLOGY OF THE POST-PHARYNGEAL
DIGESTIVE TRACT OF THE SHEEPSHEAD MINNOW, CY'RINODON VARIEGATUS (ABSTRACT).
j. MISS. ACAD. SCI. 25CSUPPL):122. (ERL.G3 X196*).
CYPRINDDCN VARIEGATUS IS A C3MMCN CYPRINQDONTI^ORM MINNOW OF THE FAST
COAST ANJ GULF OF MEXICO. THE DIGESTIVE TRACT WAS STUDIED BOTH GROSSLY
AND MICROSCOPICALLY. IT IS DIVIDED INTO ESOPHAGUS, INTESTINAL
SWELLING, INTESTIKJE PROPER, AMD RECTUM, EACH REGION HAVING A MUCOSA,
SUSMUC3SA, MUSCULARIS, AND S5R3SA. TISSUES FOR MICROSCOPY WERE
ROUTINELY STAINED dlTH HEWATQXYLIN AND EOSIN, MALLORY'S TRIPLE STAIN,
OR PAS AND EXAMINED USING BRIGHT FIELD AND PHASE CONTRAST MICROSCOPY.
CELLULAR AND NUCLEAR DIMENSIONS OF COLUMNAR EPITHELIAL CELLS, GRANULAR
CELLS, AND MJCUS-SECRETING CELLS *J5RE DETERMINES USING AN OCULAR
MICROMETER.
8ILEY, R.T., AND M.C. MIX. 1981. EFFECTS OF NAPHTHALENE ON GLUCOSE
METABOLISM i\ THE EUROPEAN FLAT OYSTER, OSTREA EDULIS. COMP. BIOCHEM.
PHYSIOL. C COMP. PHARMACOL. 7QC<1):13-20. C5RL,GB X256$).
(1). THE POOL SIZES AND RE3IST3I3UTION QF C-14 LABEL FROM
DLU-C-143GLUCQSE INTO 5THAMOL INSOLUBLE POLYSACCHASIDES, TOTAL
PROTEIN, TOTAL P3LAR LIPIDS, TOTAL NEUTRAL LIPIQS, FREE AlANINE,
ASPARTAT5, GLUTAM^TE AND TOTAL ORGANIC iCIDS WERE DETERMINED FQR
CONTROL AND NAPHTHALENE-TREATED OYSTERS DSTREA SDULIS. <2). THE
FOLLOWING EFFECTS WERE ATTRI3UTED TO NAPHTHALENE TREATMENT: A
DECREASE IN THE PERCENTAGE OF PROTEIN AND POLAR LIPID; AN INCREASE IN
THE TDTAL AMINO ACIDS CSUM IF ALANINE, ASPA3TATE AND GLUTAMATE); AN
INCREASE IN ORGANIC ACIDS? AND INCREASE IN THE MEAN SPECIFIC ACTIVITY
CGEOMETRIC) FOR ALL MEASURED POOLS. C3). T^ESE RESULTS SUGGESTED THAT
NAPHTHALENE STIMULATED PROTEIN AN3 POLAR LIPID CATABQLISM, WHILE
SIMUTAMEDU5LY STIMULATING THE =LOW 0= GLUCOSE-CARBON INTO ALL MEASURED
POOLS.
RILEY, RDNAL3 T., AND MICHAEL C. MIX. 1381. ION-EXCHANGE TECHNIQUE FOR
CONCENTRATING AMMONIA FROM SMALL VOLUMES OF ScAWATER. MAR. CHEM,
10C2):i59-160. CERL,GB X205*).
AN ION-EXCHANGE TECHNIQUE WAS USED TO CONCENTRATE AMMONIA FROM
SEAWATER. PRECIPITATION OF BIVALENT CATIONS, PRIOR TO CONCENTRATION,
REDUCED THE VARIABILITY OF AMMONIA RECOVERY FROM THE ION-EXCHANGE
COLUMN AND LENGTHENED COLUMN LIFE, ANALYSIS OF THE ELUATE WAS BY THE
PHtNOLHYPCCHLORITE REACTION. THE METHOD WAS DESIGNED FOR USE WITH
SMALL SEAWAT-R SAMPLES CLESS THAN 10 ML) AMD HAS THE ADVANTAGE THAT IT
CAN 3E ADAPTED FOR USE WITH SMALL VOLUMES OF SEAWATER WITH NO LOSS IN
SENSITIVITY. IN A 10 ML SEAWATER EXAMPLE NH(3)-N CONCENTRATIONS OF
10-150 UG LC-1) *ERE ACCURATELY DETERMINED.
z>AGE 35
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RILEY, R.T., M.C. MIX, P.L. SCHA-FrR, AND O.L. BUNTING. 1931. UPTAKE AND
ACCUMULATION OF NAPHTHALENE BY THE OYSTER OSTREA EDULIS, IN A FLOW THROUGH
SYSTEM. MAR, BIOL. 51(4):267-276.
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RILEY, RONALD T., AND MICHAEL C. MIX. 1930. THIN-LAYER SEPARATION OF CITRIC
ACID CYCLE INTERMEDIATES, LACTIC ACID, AND THE AVINO ACID TAURINE. J.
CHROMATOGR. 189(25:286-288. CERL.58 X146*).
THE IM VIVO REDISTRIBUTION OF THE C-LABSL FROM D-CU-14C3GLUCOSE HAS
8EEN STUDIED IN THE GILL TISSUE OF MARINE MOLLUSCS IN THIS LABORATORY.
ONE PHASE OF THE STUDY REQUIRED THE SEPARATION OF ORGANIC ACIDS
ISOLATED BY AN IQN-EXCHANG5 TECHNIQUE. THE METHOD 3F SEPARATION NEEDED
TO SEPARATE CLEANLY THE CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID
AND THt AMIN3 ACID TAURINE, THi SEPARATION TECHNIQUE NEFDED TO 8E
INEXPENSIVE, CAPA3LE OF HANDLING 4 FAIRLY LARGE SAMPLE VOLUME UO-20
UL), RELATIVELY RAPID, AND NEEDED TO ALL3W FOR THE EASY AND
QUANTITATIVE? RECOVERY OF SEPARATED 14C-LA3ELEO COMPOUNDS. SEVERAL
THIN-LAYER METHODS FOR SEPARATING ORGANIC ACIDS HAVE BEEN DESCRIBED
PREVIOUSLY(l-S). ONE-DIMENSIONAL METHODS SUFFER FROM LACI OF
RESOLUTION (1-3). TWO-DIMENSIONAL METHODS WHICH UTILIZED CRYSTALLINE
CcLLULDSE LAYERS <4> WERE DIFFICULT TO HANDLE AN3 EXHIBITED
CONSIDERABLE TAILING. SEPARATION OF UNDERIVATIZED 4CIDS ON SILICA GEL
LAYERS RESULTED IN CROWDING DF ACIDS NEAR THE 3RIGIN C5). ALSO, SAMPLE
REMOVAL FROM SILICA GEL LAYERS CAN BE EXCEEDINGLY TEDIOUS. THIN-LAYER
SEPARATION METHODS HAVE ALSO ?EEN DESCRIBED WHICH MADE USE OF SILICA
GEL-CELLULOSE MIXED LAYERS C2,3,S>. THIS PAPER DESCRIBES A
TWO-DIMENSIONAL MIXED-LAYER METHOD COR SEPARATING CITRIC ACID CYCLE
INTERMEDIATES, LACTIC ACID AMD THE AMINO ACID TAURINE. THE METHOD
CLEANLY SEPARATES ALL CITRIC ACID CYCLE INTERMEDIATES TESTED,
EXCEPTING CITRIC ACID AND ISOCITRIC ACID, THE SOLVENTS ARE INEXPENSIVE
AND THE METH3D ALLOWS FOR THE RAPID AND QUANTITATIVE REMOVAL OF
ISOLATED 14C-LA3ELED ACIDS BY UTILIZING A SIMPLE CELLULOSE ACETATE
STRIPPING MIXTUREC7).
RILEY, R.T. 1979. STIMULATORY EFFECT OF NAPHTHALENE ON 3LUCOSE 'TRANSPORT IN
THE OYSTER (ABSTRACT). PHARMACOL, 21:251. (ERL.GB X257*).
IT HAS BEEN DEMONSTRATED THAT THE CARBON FLUX IN BIVALVES IS
STIMULATED BY LOW LEVELS OF NAPHTHALENE. THE EFFECT OF NAPHTHALENE ON
THE GLUCOSE TRSNSP3RT SYSTEM WAS STUDIED UTILIZING
C14-ALPHA-METHYL5LJCQSIDE CMGP) AND H3-INSULIN. MGP TRANSPORT FOLLOWS
MICHAELIS-HENTON KINETICS AND IS CDMPETIVELY INHIBITED 3Y
ALPHA-3-GLUCOS'=. -I «TR DPHE MOL, PENTJCHLORQPH6NOL, CARBDNYL-CYANIDE
AND OICYCLOHSXYLCAR63DIIMI3E ALL INHIBIT TRANSPORT. qUABAIN ^ND
IODOACETATE HAVE N3 EFFECT. NAPHTHALENE STIMULATES TRANSPORT.
INHIBITORS STIMULATE DIFFUSIONAL LEAKAGE CF MGP. TRANSPORT SYSTEMS IN
THE GILL MAY -UNCTION TO MAINTAIN INTRACELLULaR DSMTTIC BALANCE. THE
MECHANISM "3Y WnlCH NAPHTHALENE STIMULATES GLUCOSE TRANSPORT IS NOT
KNOWN. HOWEVE?, IT IS HYPOTHESIZED THAT THE STIMULATORY EFFECT IS A
RESULT OF ALTERATIONS IN LIPID-PROTEIN INTERACTIONS WITHIN THE LIPIO
BILAYER. A COROLLARY TO THIS IS THAT *T HIGHER TEMPERATURE THE
MEMBRANE WTLL BECOME Li=AKY DUE TO INCREASED DISORDER 0= LIPIO PACKING.
WH=N LEAKAG5 BeC"HES GRE^T THE PART OF MSP ACCUMULATION WILL DROP.
NAPHTHALENF "LOADING SH3ULD REDUCE THE TEMPERATURE AT WHICH LEAKAGE
BEGINS. THE EFFECT OF TEMPERATURE ON MGP LEAKAGE IN NAPHTHALENE LOADED
GILLS IS CURRENTLY BEING INVESTIGATED.
PAGE 37
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-M,™< - h«!?S?J!' I8* "FECTS CP CHEMICAL PERTURBATION 3Y NAPHTHALENE
ON uLUCOSc META30LISM IN THE EUROPEAN FLAT OYSTER CQSTREA EDULIS): AN IN VIVO
KINETJE"V ™-D' DISSERTATION. OREGON STATE UNIVERSITY, CORVALLIS, OR. 173P.
(£RL,GB X053*).
THE PJRPOSF OP THIS STUDY WAS TD EVALUATE THE POTENTIAL Q* UTILIZING
AN IN VIVG KINETIC ANALYSIS 0= GLUCOSE METABOLISM AS AN APPROACH FOR
ASSESSING THc EFFECTS OF CHEMICAL POLLUTANTS ON BIVALVE MOLLUSKS. THE
RESULTS 0? THE PRESENT STUDY SUGGEST THAT THIS APPROACH COULD BE A
VALUABLE TOOL FOR EVALUATING THE LOW LEVEL EFFECTS OF CHEMICAL
PERTURBANTS ON MARINE ORGANISMS. THERE WERE THREE TYPES OF EFFECTS
EVIDENT: EFFECTS ATTRIBUTABLE TO STARVATION, EFFECTS ATTRIBUTABLE TO
EITHER REDUCED OXYGEN CONCENTRATIONS IN THE FLOW-THROUGH SYSTEM OR
DIFFERENCE IN THE GLUCOSE CONCENTRATIONS IN THE =LOW-THR3UGH SYSTEM
AND GLUCOSE INCUBATION VESSELS, AND EFFECTS DUE TO THE NAPHTHALENE
TREATMENT.
SCHNEIDER, S.R., J,D. HEMDRICKS, G.H. CONSTANTINE, AND R.E. LARSON. 1980.
TOBRAMYCIN NEPHROTOXICITY AND LETHALITY IN COHC SALMON. TOXICOL. APPL.
PHARMACJL. 54C3):399-4Q4. C5RL,GB X350),
TREATMENT Or YERSINIA RUCKERI-INFECTEQ JUVENILE CO'HQ SALMON WITH
TOBRAMYCIN CTSM) REVEALED SIGNIFICANT TOXICITY OF THE DRUG TO THESE
FISH. IN A GROUP OF FISH RECEIVING ONLY 5 ^G/KG/OAY A CUMULATIVE
MORTALITY OF 100?o WAS OBSERVED IN 21 DAYS. TO VERIFY THE SITE OF
TOXICITY, A 5ECCMD STUDY WAS CONDUCTED IN ORDER TO HI STQLOGICALLY
EVALUATE SPECIFIC TISSUES AN3 TO DETERMINE DOSE-RESPONSE
CHARACTERISTICS. T5M PRODUCED NEPHOTQXICITY IN JUVENILE COHO SALMON AT
DOSAGES ONLY HALF AS GREAT AS THOSE REQUIRED FOR AN EQUIVALENT EFFECT
IN MAMMALS AMD DID SO OVER A MUCH SHORTER TREATMENT COURSE.
HISTOLOGICAL EXAMINATION REVEALED MODERATE TQ COMPLETE PROXIMAL TUBULE
NECROSIS AT 7,5 MG/KG EVERY OTHER DAY FOR 5 DAYS. LETHALITY OCCURRED
AT DOSAGES AS LDW AS 2 MG/KG/DAY FOR 12 D4YS. THE DATA APPEAR TO
INDICATE TH'iT SALMON MAY BE A POTENTIAL MODEL FOR STUDYING THE
NEPHROTOXIC POTENTIAL OF CHEMICALS.
SCHOOR, *l. PETER. IN PRESS. EXPOSURE OF FISHES TO BENZ DC A) PYRENE AND SOME
ASPECTS OF ANALYSIS OF METABOLITES. IN: PROCEEDINGS OF THS SYMPOSIUM ON THE
USE OF SMALL FISH SPECIES IN CARC JNOGENICITY TESTING, 3ETHESDA, MARYLAND,
DECEMBER 8-10, 1931. U.S. NATIONAL CANCER INSTITUTE. CERL,GB 454).
THE DISPOSTICN OF BENZOC A)PYR ENE £B(A>P] IN MOST ANIMAL SPECIES
DEPENDS LARGL-Y ON THE EFFECT THAT THE MIXED FUNCTION OXYGENASE (MFO)
AND TRANSFERASE SYSTEMS EXERT ON IT. SINCE THESE ENZYMES ARE INDUCIBLE
BY COMPOUNDS SUCH AS PHENDBAR3ITAL C?B) AND 3-METHYLCHOLANTHRENE
U-MC), MUCH EFFORT WAS SPENT TO DETERMINE THEIR EFFECTS ON THE ENZYME
SYSTEMS PR-S=NT IN SOME AQUATIC SPECIES. IN THE SPECIES TESTED SO FAR
CMULLET, SEA CAT-ISH, AN3 KILLIFISH), INDUCTION WAS FOUND WHEN THE
INDUCER Was ADMINISTERED INTRAPERITDN? ALLY. FLOW-THROUGH EXPOSURE TO
PB ALS3 IN3UCE3 MFQ ACTIVITY IN MULLET AND KILLIFISH. MFO ACTIVITY WAS
DETERMINED USING BCA)P AS SU3STRATE AND THE RESULTING METABOLITES HAVE
BEEN S^PAR^D. TH= PROBLEMS ASSOCIATED WITH THESE DETERMINATIONS ARE
DISCUSSED IN LIGHT 3F USING DATA ON AN INTER-LA 30?? ATORY BASIS.
PAGE 38
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SCH30R, H. P-T=R, AND MEERA SRIVASTAVA. 1983. EFFECTS DF TRICHLQROPRQPENE
OXIDE ON THr METABOLISM DF 3F.NZDC O?YRENE 8Y 3-METHYLCHOLANTHRENE- AND
PHENOSARBITAL-TREUED MULLET (MUGIL CEPHALUS), A MARINE FISH (ABSTRACT). IN:
PROGRAM AND ABSTRACTS DF FIRST INTERNATIONAL SYMPOSIUM ON FOREIGN COMPOUND
METABOLISM, OCT. 30 THRU NOVEMBER 4, 1983, WEST PALM 3EACH, FLORIDA. 3Y ISDLAT5D LIVER MICROSOMES WERE EVALUATED. THE
MOST SINGIFICANT EFFECT FCUNO WAS AN INCREASE IN THE OXIDATION OF BAP
AT THE 3AY REGION (POSITION 9 AND 10) IN =ISH WHICH WERE PRETREATED
WITH 3-MC AND P3. NO SIGNIFICANT DIFFERENCES WERE FOUND IN THE
METABOLIC PATTERNS DF 3-MC- AND PB-TR5ATED FISH. WHEN METABOLITE
PATTERMS Or THE DHYDRODIOLS IN THE PRESENCE AND ABSENCE OF TCPO WERE
COMPARED, THE PREDICTED SHIFTS WERE OBSERVED IN CONTROL AS WELL AS
3-MC- AND PB-TREATED ANIMALS. COMPARISON OF METABOLITE PATTERNS IN THE
PRESENCE OF TC°0 SHOWED DISTINCT DIFFERENCES BETWEEN CONTROL AND 3-MC-
AND P3-TREATEJ ANIMALS. NO SIGNIFICANT DIFFERENCE IN METABOLITE
PATTERNS WERE FCJND 3ETWSEN 3-MC- AND PS-TREATED ANIMALS IN EITHER
PRESENCE DR A3SENCE C= TCPO. COMPARISON Tl MAMMALIAN DATA SHOWED THE
PATTERN IN FISH TO aE VERY
INDUCTION-RELATED METABOLITE
THAT FDUMD IN RATS.
SIMILAR TO
SCHQOR, rf. PETER, AND MEERA
LIVER MICROSOMES: EFFECT 0=
AT 25 DEGREES AND 37 DESREES
INTERNATIDNAL SYMPOSIUM ON
27-29, 1983, WQDDS HOLE, MA.
SRIVASTAVA. 1983. INDUCTION OF MFO IN MULLET
NAOPH ON 8cNZO(A)PYREN= METABOLITE DISTRIBUTION
(ABSTRACT). PRESENTED AT THE SECOND
POLLUTANT RESPONSE 1*1 MARINE ORGANISMS. APRIL
(ERL,GB 166*).
MIXED-FUNCTIDN QXYGENASc ACTIVITIES IN MULLET CMUGIL CEPHALUS) WERE
INDUCED WITH 3-METHYLCHDLANTHREN£ AND PHENQBAR3ITAL (PB) ADMINISTERED
INTRAPERITONtALLY TWICE OVER A THIRTY-DAY PERIOD, CONTROL AND INDUCED
MFD ACTIVITIES W~RE STUDIED 4T 25 DEGREES AND 37 DEGREES CELSIUS USING
0.1 - 4.0 MM NADPH. INCREASES IN NAOPH CONCENTRATIONS IN ACTIVITY
DETERMINATIONS CF CO.MTRCL MULLET MFO AT 30TH TEMPERATURES SHOWED
CORRESPONDING ACTIVITY INCREASES AND RATHER BROAD ACTIVITY OPTIMA. NO
SIGNIFICANT CHANGES IN THE METABOLITE COMPOSITION WERE OBSERVED. MFQ
ACTIVITIES INDUCED BY 3-MC AND CONDUCTED AT 37 DEGREES CELSIUS SHOWED
INCREASES OF ALL METABOLITES EXCEPT THE 4,5-EPOXIDS AND 5-PHENOL WITH
INCREASING NADPH CONCENTRATIONS. NO SUCH INCREASES WERE FOUND AT 25
DEGREES CELSIUS. INCREASES IN THE INDIVIDUAL METABOLITES WERE
DISPROPORTIONATELY HISH'ER AT 37 DEGREES CELSIUS AND HIGH NADPH
CONCENTRATIONS WH=N COMPARED AT 25 DEGREES CELSIUS. SIMILAR TRENDS
WERE F3UND WITH THC PB INDUCED MFQ ACTIVITIES. THE DATA INDICATE THAT
WITH R-GARD TD CTMPARATIVE INDUCTION, PHYSIOLOGICAL TEMPERATURE
RANGES" MAY MA
-------
ELNENAEY- 1^30. METABOLITES 0- SENZ0(A>PYRENE
PROPERTIES AND DETECTION (ABSTRACT).
5-CTICN D-p TH? AMERICAN CHEMICAL SOCIETY, TAMPA, FL,
INDIVIDUAL METABOLITES OF BENZO(A)?YRSN£ WERE SEPARATED 3Y HIGH
PRESSURE LIQUID CH?OMATOGRAPHY WITH METHANIL/WAT^R GRADIENTS.
EXCITATION AND EMISSION SPECTRA WERE OBTAINED FOR EACH PEAK AFTER
STOPPING THE HPLC FLOW AMD COMPARED TO THQS? 3F PUR? STANDARDS. ALL
TWELVE PHENOLS Or 3ENZCCAJPYRfcNE SHOWED DISTINCTLY DIFFERENT
EXCITATION SPECTRA WHILE SHIFTS IN THE EMISSION SPECTRA 'WERE NOT
UNIQUELY DIFFERENT. THE PHENOXIDES DID NOT YIELD DISTINCT SPECTRA. THE
QUINONES AS WELL AS THE DIOLS SHOWED DIFFERING SENSITIVITIES TOWARDS
LIGHT. CERTAIN! REVERSE PHASE COLUMNS APPEAR TO HAVE THE TENDENCY TO
REACT WITH PHENOLS CAUSING EITHER 3INDING OR OTH=R CATALYZED
REACTIONS.
SCHOQR, W. PETcR, AND JOHN A, COUCH. 1979. CORRELATION OF MIXED-FUNCTION
OXIDASE ACTIVITY rflTH ULT.
SPECIMEN Cp MULLET (MUGIL CEPHALUS), A MARINE FISH, W£*E INDUCED WITH
A SINGLE DOS£ OF 3-MrTHYLOCHOLANTHRENE (30 MG/KG) INJECTED
INTRAPERITONEALLY AMD SACRIFICED AT DIFFERENT TIMES. THE ISOLATED
LIVER MIC^OSOMAL FACTION WAS ALLOWED TO METABOLIZE 8ENZQPYRENE?
THE RESULTING PRODUCTS WERE ISOLATED BY EXTRACTION, AND 3U&NTITATED BY
HPLC COUPLED WITH ULTRAVIOLET AND FLUORESCENCE DETECTCRS. EXICITATION
AND EMISSION SPECTRA OF A NUMBER OF 8A? METABOLITE STANDARDS WERE
OBTAINED WHICH WERE USED IN IDENTIFYING PEAKS OBTAINED FROM THE
METABOLISM EXPERIMENTS,
PAGE 40
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SMlTHf A.C., AND M.C. MIX. 1978- EFFECTS Q- SODIUM CHLORlD^ CONCENTRATION
ON ELECTRDPHQRETIC PATTERNS 0- ADDUCTOR MUSCL- PRGTrlNS FROM BIVALVE
MOLLUSCS. COM?. BIOCHEM. PHYSI3L. 8 COMP. 3IDCHEM. 513C1):169-171.
-------
STRr ,GLc. * °'V* SAR4DAM8AL, SHOO-LIH WANG, AND H.H. OARON. 1982.
GLUCURONSYL- AND SULFQ- TRANSFERA5ES IN rISH EXPOSED TO ENVIRONMENTAL
CARCINOGEN!. -ED. P3QC. 4l:iU7.
-------
SULLIVAN, TIMOTHY J. , AND MICHAEL C. MIX. 1983. PYROLYTIC DEPOSITION OF
PQLYNUCLEAR AR3MATIC HYDROCARBDNS DUE TO SLASH BURNING ON CLEAR-CUT SITES.
BULL. ENVIRON. CQNTAM. TOXICOL. 31C2>1208-215. CERL,G? X445).
THE C3JECTIVES OF THIS RESEARCH WERE TO IDENTIFY QUALITATIVELY AND
QUANTITATIVELY THE DEPOSITION, PERSISTENCE AND FATE OF SELECTED
UHSUBSTITUTED »NAH COMPOUNDS QM 3URNED CLEAR-CUT SITES IN CREGON. THE
PRESENT PAPER PRESENTS DATA ON THE INITIAL DEPOSITION OF PNAH AFTSR
SLASH 3U3NING. A SUBSEQUENT PAPER WILL CONSIDER THE FATE AND
PERSISTENCE OF THE PNAH PRODUCED.
SULLIVAN, TIMOTHY J.t AND MICHAEL C. MIX. 1983. SIMPLE AND INEXPENSIVE
METHOD FO-? MEASURING INTEGRATED LIGHT ENERGY. ENVIRON. SCI. TECHNQL.
17(2):127-128, CERL.GB X461).
THE QZALID TECHNIQUE IS A SIMPLE AND INEXPENSIVE METHOD FOR MEASURING
INTEGRATED SUNLIGHT ENERGY IN THE FIELD FQ3 PERIODS UP TO A MAXIMUM OF
1 DAY. THIS 3APER DESCRIBES A MODIFICATION OF THE OZALID TECHNIQUE
THAT MAKES IT SUITABLE FOR L?NG-TESf LIGHT MEASUREMENTS. DATA FROM THE
MODIFIED OZALID METSR WERE CALIBRATED AGAINST AN EPLEY PRECISION
SPECTRD PYRANOMETER, YIELDING A STRONG POSITIVE CORRELATION
CRCSQUARED) = 0.97).
TAN, BARRIc, AND PAUL MELIUS. 1982. BENZ3CA3PVRENE METABOLISM IN HEPATIC
S-9 FRACTIONS Or AROCLCR 1254-TREATrD MULLET CMUGIL CcPHALUS). IN:
POLYNUCLEAR AROMATIC HYDROCARBONS: PHYSICAL AND BIOLOGICAL CHEMISTRY, M.
COQKE ET AL. , EDITOR, BATTELLE PRESS, COLUMBUS, OH. PP. 801-811.
-------
TAN, 3ARRIE, PAUL McLIUS, AND PAUL ZIEGLER. 1982. SIMPLE GAS
CHROMATOGSAPHIC METHOD FOR THE STUDY OF ORGANIC SOLVENTS: MOISTURE ANALYSIS,
HYGROSCOPICI7Y, AND EVAPORATION. J, CH3CNATC3R. SCI. 20(5):213-217.
(ERL,G8 X278*).
THIS PAPER PRESENTS THz 3ASELI«5 SEPARATION OF CIG-!T COMMON LABORATORY
ORGANIC SOLVENTS BY GC WITH TCO AND SIMPLE ISOTHERMAL CONDITIONS, THE
INFLUENCE OF COLUMN TEMPERATURE ON THE CH3QMATCSRAPHIC PARAMETERS,
CAPACITY FACTOR (K), AND RESOLUTION (R), WAS STUDIED. RAPID ANALYSIS
OF MOISTURE IN DR3A.MIC SOLVENTS WAS DEMON ST3ATED. A LINEAR DETECTION
RESPONSE OF 100 PPM TO 30% (V/V) WAS SHOWN FCR WATER IN ETHANOL-
SOLVENT SAMPLES PLACED IN OPEN BEAKERS ABSORBED WATER AT VARYING
RATES, THri RELATIVE RATE 3F HYGROSCQPICITY WAS ETHANOL, 2-PROPANOL,
ACETONITRILE ETHYL ACETATE, 4CETONS GREATER THAN MHTHAMQL GREATER THAN
CHLOROFORM, SOLVENT SAMPLES, STORED IN STOPPERED VOLUMETRIC FLASKS OR
SEPTUM-CAPPEO VIALS ANO PERIODICALLY SAMPLED, GAVE NO SIGNIFICANT
MOISTURE ABSORPTION, A MIXTURE OF ORGANIC SOLVENTS IN A SLOWLY STIRRED
OPEN 3EAKER INDICATED DIFFERENCES IN ATMOSPHERIC EVAPORATION. THE
RELATIVE RATE OF EVAPORATION WAS ETHER » ACETONE, CHLOROFORM,
ACETONITRILE GREATER THAN METHANQL, ETHYL ACETATE GREATER THAN
ETHANOL, 1-PROPANOL »WATER.
TAN, BASRIE, PAUL MELIUS, AND JOHN GRIZZLE. 1981. HEPATIC ENZYMES AND TUMOR
HISTQPATHOLOGY OF BLACK BULLHEADS WITH PAPILLQMAS. IN: CHEMICAL ANALYSIS
AND BIOLOGICAL FATE: POLYNUCLEAR AROMATIC HYDROCARBONS. MARCUS COOKE AND
ANTHONY J. DENNIS, EDITORS, 8ATT5LLE PRESS, COLUMBUS, OH. PP. 377-386,
(cRL,G8 X276*).
IN THIS STUDY, AN UNUSUALLY HIGH INCIDENCE OF PAPILLOMAS (70+-5S) IN
BLACK 3ULLHEADS CICTALURUS MELAS) FOUNO IN A T.JO-ACRE FINAL OXIDATION
SEWAGE POND. THE UNIQUE FEATURE WAS THAT THE AERATION RESPONSIBLE FOR THE HIGH TUM05? INCIDENCE.
TAN, BASRIE, AND PAUL MELIUS. 1981. INDIRECT ATOMIC ABSORPTION
SPECTROMETRIC ASSAY FOR gPOXIDE HYDROLASE, ANAL, LETT. 14(85):311-322.
{ERLjGB X282*).
AN INDIRECT ATOMIC ABSORPTION SPECTROMETRIC ASSAY FOR EPOXIDE
HYDROL4SE WAS REPORTED. THE ENZYMATIC PRODUCT 1,2-OIOL WAS OXIDIZED BY
AN EXCESS 3F POTASSIUM PERIOOATE, AND THE UNCDNSUMED PERIOOATE WAS
PREFERRENTIALLf PRECIPITATED 3Y LEAD PERI30AT5. THE PRECIPITATE WAS
ACID DIGESTED FOLLW=D BY ATOMIC ABSORPTION DETERMINATION OF LEAD IN
THE RESULTING SOLUTION. TRANS-STIL8ENE OXIDE CTSO) INDUCED RABBIT AND
TILAPIA EPOXIDE HYDROLASE ACTIVITIES BY 350* »ND 210% AT THE 490 MG
TSO/KG TREATMENT WHILE AROCL3R 1254 CAR) ONiLY SLIGHTLY INCREASED THEIR
ACTIVITIES AT THE 200 MG AR/KG TREATMENT, THE
l,2-ePOXY-3,3-TRICHLOROPROPANE CTCPO) AT 3.1 MM COMPLETELY INHIBITED
TILAPIA (CONTROL) AND RAB3IT (490 MS TSO/KG) EPOXID5 HYOROLASE
ACTIVITIES.
PAGE 44
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TAN, BAJ«;» "fLVlN V. KILGORE, DAVID L. :LAM, PAUL MELIUS, AND W,P. SCHOOR.
1981. METABOLITES Or BENIG(A)PYREN= IN AROCLDR 1254 TREATED MULLET. IN:
AQUATIC TOXICOLOGY AND HAZARD ASSESSMENT, ASTM ST? 737. D.R. BRANSON AND
K.L. DICKSON, EDITORS, AMERICAN SOCIETY F?R TESTING AND MATERIALS,
PHILADELPHIA, PA. PP. 239-246. (E3L,G3 X267«O,
THIS PAPER PRESENTS A SYSTEMATIC APPROACH TO THE S-PcRATION AND
CHARACTERIZATION OF 3(A)P METABDLITES USING Ti£ E-FICIENT ANALYTICAL
TECHNIQUE OF HIGH PRESSURE LIQUID CHROMATDGRAPHY (H<>LC). HEPATIC S-9
PREPARATIONS = RQM ARCLDR 1254 TREATED MULLET (MUSIL CEPHALUS> WERE
USED TO STUDY THE INVITRO MrTABOLISM OF 3(A)P. THE LIPID-SOLUBLE
HYDRCXYLATED PRODUCTS WERE EXTRACTED IN ETHYL ACETATE PRIOR TO HPLC
ANALYSIS. THREE DIOLS (9,10; 7,3; AND 4,5) AND TWO PHENOLS (9 AND 3)
OF B(A)P WERE ISOLATED AND IDENTIFIED. FROM THEIR UNRSSOLVABLE
QUINGNES. THE RESULTS OBTAINED IN THE MULLET WERE COMPARED TO THOSE
OBTAINED IN THE RAT (RATTUS NQRV5GICUS).
TAN, BAR3IE, AND PAUL MELIUS. 1981. RESPONSES OF THE HEPATIC ENZYMES OF A
TtLEOST FISH TO TR ANS-STIL3ENE OXIDE TREATMENT. 3ULL. ENVIRON. CONTAM.
TOXICOL. 26(6):801-806. (ERL,38 X325).
THE MICRDSDMAL MIXED FUNCTION 3XIDASE F STYREME EDOXIDE. THIS IS DF PARTICULAR INTEREST BECAUSE
EPOXIDE HYORASE CATALYZES THE HYDROLYSIS Oc AN E?CXIDE TO A 1,2-DlDL.
THE PROCEDURE MAY LEND ITSELF TO THE DETERMINATION OF THE ENZYME
ACTIVITY OF EPDXIDE HYCRA3E, KNOWN FOR ITS PIVOTAL ROLE IN THE
META30LISM OF 'WJY CARCINOGENS AND TOXIC COMPOUNDS,
PAGE 45
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TRENHQLM, STEVE R., AND MICHAEL C. MIX. 1973, REGENERATION 3F
RADIATION-DAMAGED DIGESTIVE TISSUES IN JUVENILE PACIFIC OYSTERS CCRASSOSTREA
GIGAS). J. INVERTEBR, PATHQL. 32O) : 249-257. C?RL,GB X154*).
JUVENILE PACI-IC 3YSTERS, CRASSOSTRSA GIG4S, WERE IRRADIATED WITH 16
AND 40 KRAD AND THEIR TISSUES EXAMINED HiSTOLQGICALLY. DEGENERATIVE
SYNDROMES AMD TISSUE REGENERATI3N PROCESSES WERE DETERMINED FOR THE
STQMAC-it GUT, COLLECTING DUCTS, AND DIGESTIVE TUBULES- FOLLOWING
DEGENERATION, TISSUE REGENERATION WAS OBSERVED IN THE DIGESTIVE
TISSUES OF MOST CYSTSRS EXPOSED TO 15 KRAD AND IN A LIMITED NUMBER
EXPOSED T3 40 K'RAD. REGENERATION WAS CIRST OBSERVED IN THE DIGESTIVE
TUBULES AND SUBSEQUENTLY IN T^E STOf^ACH, GUT, AMD COLLECTING DUCTS.
CELLULAR R5P3PULATION 3F THE DIGESTIVE TUBULES INVOLVED
EPITHELIALIZATION WITH LARGE, UNDIrFERENTIATED CRYPT CELLS WHICH THEN
DIFFERENTIATED IMTQ FUNCTIONAL SECRETORY AMD ABSORPTIVE CELLS.
REGENERATION IN T^ STOMACH, SUT, AND COLLECTING DUCTS WAS INITIATED
BY PROLIFEPATIVE ISLANDS OF SMALL BASOPHILIC CELLS. MIQTIC DIVISION OF
THOSE CELLS &NO THEIR SUBSEQUENT DIFFERENTIATION INTO FUNCTIONAL
EPITHELIAL CELLS RESULTED IN THE RAPID RESTDRATION AND APPARENT
RECOVERY OF THE AFFECTED TISSUES. THE RESULTS DF THESE STUDIES
INDICATE THAT RADIDRESISTANCS OF JUVENILE C. GIGAS MAY IN PART BE DUE
TO THE REMARKABLY EFFICIENT REGENERATIVE MECHANISMS INVOLVED IN
REPLACING INJURED 3R LOST DIGESTIVE TISSUES.
PAGE 46
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TRENHOLM, STrVE RAY. 1977, EFFECTS 0* x- AND GAMMA IRRADIATION ON THE
JUVENILE PACIFIC OYSTER, CRASSOSTREA GIGAS. M.S. THESIS. OREGON STATE
UMIVERSITY, CORVALLIS, OR. 75P. (ERL,GB
TWO SEPARATE STUDIES WSR£ CDMDJCTED DURIMG THIS RESEARCH PROJECT.
OYSTERS dERE IRRADIATED WITH 500 AND 1000 RADS AND 5, 10, 20, 50, 75,
100, AND 200 KRADS IN THE FIRST STUDY IN ORDER TO DETERMINE THE
EFFECTS Qr IONIZING RADIATION ON SURVIVAL AND GSOWTH RATES. TWO
PERIODS Cr HIGH MORTALITY WERE NOTEO? THE FIRST OCCURRED FROM 2 TO 7
DAYS POSTIR3AOIATION IN OYSTERS DECEIVING 75 TO 200 KRADS ANO WAS
ASSOCIATED UITH AN "ACUTE LETHAL TISSUE DEGENERATIVE SYNDROME." THE
SECOND MORTALITY PERIOD OCCURRED FROM 40 TO 60 DAYS PC STIRRADIATIQN IN
OYSTERS EXPOSED TO 10 KRADS OR MORE AND »JAS CAUSED BY A "LETHAL TISSUE
DEGENERATIVE SYNDROME." THE LD-50 DOSE rfAS FQUNO TO 3E A COMPLEX
FUNCTION C:- TIME F*OM THE MOMENT OF IRRADIATION UNTIL APPROXIMATELY 80
DAYS POSTIRRIDIATION. THE 238-DAY LD-50 VALUE WAS 16.5 KRADS. THE MEAN
WET WEIGHT OF OYSTERS EXPOSED TC 20 KRADS WAS SIGNIFICANTLY LESS THAN
THAT OF THE COMTRDLS FROM 167 TO 238 CAYS =>OSTIR« AOIATIQN. ANALYSIS OF
THE RESULTS SUGGEST A DOSE DEPENDENT WET-WEIGHT RELATIONSHIP IN THE 5
AND 10-KRAD QYSTE?S; THEY DID NOT WEIGH SIGNIFICANTLY LESS THAN THE
CONTROLS. ALTHOUGH NOT STATISTICALLY SIGNIFICANT, THE MEAN WET WEIGHTS
OF OYSTERS EXPOSED TO 500 AND 1000 RADS EXCEEDED THAT 0*= THE CONTROLS
FROM 43 TO 238 DAYS PC STIRRAOI ATION, IN THE SECOND STUDY, OYSTERS MERE
IRRADIATED WITH 200 R, 1000 3, 5KR
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TITLE INDEX
PAGE 43
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KEYWORD TITLE INDEX P T
A T
G 5
E M
A
ASILITY OF AMBYSTOMA TIGRlNUa HEPATIC MICROSDMES FROM POLYCYLIC AROMAT 1- 1
ABSORPTION WITH DIGESTED LEAD PE3I3DATE /, DETERMINATION OF 1,2-DIOLS B 45- 3
SPECTROMETRTC ASSAY FDR EPCXIDS HYDROLASE /.INDIRECT ATOMIC 44- 3
ACCUMULATION OF MAPHTHALENS BY THE GYSTES '3STREA EOULIS, IM A FLOW THR 36- 1
ACID TAURIME /.THIN-LAYER SEPARATION OF CITRIC AGIO CYCLE INTERMEDIATE 37- 1
« AND THE AMINO ACID TAURINE /,THIN-LAYER SEPARATION OF CITRIC ACI 37- 1
» CYCLE INTERMEDIATES, LACTIC ACIO, AND THE AMINQ ACID TAURINS /,TH 37- 1
ACTIVATION /.DETERMINATION OF VANADIUM IN 4 MARINE MOLLUSC USING A CHE 20- 3
ADBUCTOR MUSCLE PROTEINS FROM BIVALVS MOLLUSCS /•EFFECTS Op SODIUM CHL 41- 1
ADVANTAGES AND S»SCISL PR03LSMS /.USEFULNESS Oc CYPRINOD1N VARIEGATUS 13- 1
AH.ATQXIN ON THE BROWN BULLHEAD ICTALURUS NE3ULOSIS. M.S. THESIS /,EF 2- 1
ALTERATIONS IN CARCINOGEN-EXPOSED TELEOSTS: PROCEDURES FOR PREPARATIO 24- 1
AMBYSTQMA TIGRINUM HEPATIC MIC30S01ES = ROM PQLYCYLIC AROMATIC HYDROCAR 1- 1
AMERICA /.CELLULAR PROLIFERATES DISORDER IN OYSTERS OSTREA CHILENSIS 30- 1
AMERICAN OYSTER CCRASS^STREA VIRGINICA) AS AN INDICATOR OF CARCINOGENS 11- 1
" OYSTER CRASSOSTREA VIRGINICA /,BENZO(A)PYR£NE MET&30LISM IN T 1-2
«« OYSTER (CRASSOSTREA VIRGINICA) /, CONCURRENT" NEOPLASTIC ANO PR 11- 2
AMINES /.DIFFERENTIAL ABILITY JF AM3YSTQMA TIGRINUM HEPATIC MICROSOMES 1- 1
AMINO ACID TAURINE /.THIN-LAYER SEPARATION OF CITRIC ACID CYCLE INTERM 37- 1
AMMONIA FROM SMALL VOLUMES OF SEAWATER /,I3N-EXCHAMG£ TECHMIQUS FOR CO 35- 3
ANIMAL MODEL FOR EXPERIMENTAL CARCINOGENESIS /iRAINBOW TROUT CSALMO GA 18- 1
" IN STUDIES INVOLVING CARCIN3GENESIS, TERAT05ENESIS AND MUTAGEME 19- 1
ANIMALS AS INDICATORS OF ENVIRONMENTAL EXPOSURES /.A3UATIC 6- 1
11 /.EFFECTS 0? CARCINOGENS, MUTAGENS, AMD TERATOGENS ON NON-HUM 6- 2
11 /.EFFECTS OF Ca.RCIMOGENS. MUTAGENS AND TERATOGENS ON NON-HUMAN 7- 1
11 : FQUR^ ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM /.EFFECT 5- 2
" /.EFFECTS OF CARCINOGENS, MUTAGENS, AND TERATOGENS ON NON-HUM 6- 2
11 (A3STRACT) /.EFFECTS OF CARCINOGENS, MUTAGENS, AND TER«TOGENS 4- 1
" /.EFFECTS C- CARCINOGENS, MUTAGENS, AMD TERATOGENS ON'NON-HUM 6- 2
" /.INDEX AND ABSTRACTS TO PUBLICATIONS, THE EPA-NCI PROJECT: 14- 2
" INDUCED 3Y CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTAMINANTS, 26- 3
ANOMALIES THAT OCCJR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT CHEMI 34- 2
APPLICATION IN CARCINOGEN EXPOSURE STUDIES /.PRIMARY CELL CULTURES FRO 22- 1
AQUATIC ENVIRONMENT /.AMERICAN OYSTER CCRASSOSTREA VIRGINICA) AS AN IN 11- 1
" ANIMALS AS INDICATORS QF ENVIRONMENTAL EXPOSURES 6- 1
" ANIMALS) /.EFFECTS OF CARCINOGENS, MUTAGENS, AND TERATOGENS ON 6- 2
11 ANIMALS /.EFFECTS OF CARCINOGENS, MUTAGENS AND TERATOGENS ON N 7-1
" ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM 5- 2
11 ANIMALS) /, EFFECTS OF CARCINOGENS, MUT5G5NS, AND TERATOGENS ON 6- 2
" ANIMALS (ABSTRACT) /.EFFECTS 0?^CARCINOGENS, MUTAGENS, AND TER 4- 1
11 ANIMALS) /.EFFECTS Or CARCINOGENS, MUTAGENS, AND TERATOGENS ON 6- 2
11 ANIMALS) /,INDEX AMD ABSTRACTS TO PUBLICATIONS, THE EPA-NCI PR 14- 2
" ANIMALS INDUCED 3Y CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTA 26- 3
ARDCLOR 1254-TREATED MULLET (MUGIL CEPHALUS) /,BENZOCA3PYRENE META30LI 43- 3
11 1254 TREATED MULLET /.METABOLITES OF BENZO
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KEYWORD TITLE INDEX ? i
A T
3 E
5 M
A
ASSAY FOR EPOXIDi HYDROLASE /.INDIRECT ATOMIC ABSORPTION S°-CTROMETRIC 44- 3
» iUoJrCTS /.LABORATORY EVALUATION DF MARINE FISHES AS CARCINOGEN 9- 1
ATOHIC ABSORPTION WITH DIGESTED LEAD PSRICOATE /, D =TFRHI,NATlQN OF 1,2- 45- 3
« ABSORPTION SPECTRGMETRIC ASSAY FDR EPOXIDE HYDRQLASE /.INDIRECT 44- 3
B
BACTERIA SYSTEM. M.S, THESIS /..EVALUATION OF 3ENZO(A)PYRENE METABQLIS 2- 2
BANDING OF CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH C=LL LINES / 15- 1
BAP METABOLISM IM MULLET CABSTRACT) /,EFFECT 0^ 2,3-OXIDP-3,3,3-TRICHL 41- 2
BARNACLE, POLLICIPES POLYMERUS FOR MARINE 3IOASSAYS /.PRELIMINARY STUD 33- 1
BAY, OREGON OURING JUNE 1976 - MAY 1973 /,8ENZO(A)PYRENE CONCENTRATION 31- 2
» MUSSELS, MYTILUS EOULIS /,BENZOCA)?YRENE CONCENTRATIONS IN SOMATIC 28- 2
« MUSSELS CMYTILUS EDULIS) FROM OREGON, USA /.CONCENTRATIONS OF UNSU 27- 2
» CREGOM, USA /.CONCENTRATIONS OF UMSUBSTITUTED POLYCYCLIC AROMATIC 27- 1
« MUSSELS /,EFFECTS OF DEPURATION, SIZE AND SEX ON TRACE METAL LEVEL 20- 1
• MUSSELS, MYTILUS EDULIS, FROM OREGON: OCCURRENCE, PREVALENCE, SEA 27- 3
» OREGON /.OBSERVATIONS ON THE ULTRASTRUCTURE OF LARGE CELLS ASSOCI 32- 2
• MUSSELS (MYTILJS EOULIS) FROM OREGON /,POLYNUCLEAR AROMATIC HYDROC 29- 2
« MUSStL, MYTILUS EDULIS /,SEASONAL VARIATION IN SOFT TISSUE WEIGHTS 20- 4
11 MUSSELS (MYTILJS EOULIS) /.SEASONAL VARIATIONS OF ARSENIC AND OTHE 20- 2
" OREGON. PH.D. THESIS /.TRACE METAL LEVELS IN A POPULATION! OF MYT 21- 1
BESZIDINE TQXICITY (ABSTRACT) 22- 3
BENZO(A)PYRENE BODY 3URDEKS AND THE PREVALENCE OF PROLIFERATIVE OISORD 31- 1
» A)PYRENE CONCENTRATIONS IN MUSSELS CMYTILUS EDULIS) FROM YAQUINA 31- 2
11 A)PYR£NE CONCENTRATIONS IN SOMATIC AMD G3NAD TISSUES OF 3AY MUSS 28- 2
" A)PYRENE METABOLISM IN THE AMERICAN OYSTER CRASSOSTREA VIRGINICA 1- 2
* A3PYRENE METABOLISM IN HEPATIC S-9 FRACTIONS OF 4ROCLOR 1254-TRE 43- 3
" A)PYRENE IN ECONOMICALLY-IMPORTANT BIVALVE MOLLUSKS FROM OREGON 33- 2
" A)PYRENE METABOLITE PATTERNS IN FISH AND RODENTS /.COMPARATIVE 24- 2
H A)PYRENE BY 3-METHYLCHOLANTHRENE- AND PHENOBARBITAL-TREATED MULL 39- 1
" A)PYRENE METABOLISM IN AN OYSTrR COSTREA EOULIS>-3ACT£RIA SYSTEM 2~ 2
" A)PYRENE AND SOME ASPECTS OF ANALYSIS OF METABOLITES /.EXPOSURE 38- 3
" A)PYRENE FROM A MARINE FISH CA8STRACT) /,FLUORIMSTSIC CO^FIRMATI 40- 3
fl A)^YRENE METABOLITE DISTRIBUTION AT 25 DEGREES AND 37 DEGREES C 39- 2
11 A)PYRENE (ABSTRACT) /, LIVER NEQPLASIA AND INDUCTION OF HEPATIC M 17- 1
" A)PYRENE FSC'1 MULLET (MU5IL CEPHALU5): PROPERTIES AND DETECTION 40- 1
11 A)PYRENE INi AROCL03 1254 TREATED MULLET /.METABOLITES OF 45- 1
11 A)PYREME BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY /.SEPARATION ' 13- 2
" A)PYRENE METABOLITES (ABSTRACT) /.SIMPLE HIGH PERFORMANCE LIQUI 14- 1
BIQASSAYS /.PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMB 33- 1
BIOCHEMICAL CHARACTERISTICS /.CCf^PLEX CHROMATOPHOROMA IN A MARINE TELE 3- 1
BIOMONITORING OF COASTAL WATERS—AW OVERVIEW 10- 1
BIVALVE MOLLUSKS FROM OREGON ESTJA3IES /.CHEMICAL CARCINOGENS IN 32- 1
" MOLLUSKS FROM OREGON ESTJARIES /.CHEMICAL CARCINOGENS IN THE M 33- 2
" MOLLUSCS /,EFFECTS OF SCDIUM CHLORIDE CONCENTRATION ON ELECTRO 41- 1
11 MOLLUSCS FROM CR-GON ESTUARIES /.POLYNUCLEAR AROMATIC HYDROCAR 29- 1
11 MOLLUSKS IN ".AY MUSSELS (MYTILUS EDULIS) FROM OR6GON /,POLYNUC 29- 2
BUCK BULLHEADS WITH PAPILLQMAS /.HEPATIC ENZYMES AND TUMOR HISTOP4THQ 44- 2
" BULLHEADS,* ICTALURUS MELAS (RAFINESQUE), LIVING IN A CHLORINATED 15- 3
BLOOD CELLS OF CYP*INOOQN VARIEGATUS (ABSTRACT) /.MORPHOLOGY OF PERIPH 21- 2
BUDDED VERTEBRATES /.HEMATQPOIETIC NEOPLASMS IN INVERTEBRATES AND COL 16- i
BODY BURDENS AND THE PREVALENCE OF PROLIFERATIVE DISORDERS IN MUSSELS 31- 1
BROHM BULLHEAD ICTALURUS NEBULOSIS. M.S. THESIS /.EFFECTS OF AFLATOXI 2- 1
BULLHEAD ICTALURUS NEBULOSIS. M.S. THESIS /.EFFECTS DF AFLATOXIN ON T 2-1
BULLHEADS WITH PAPILLOMAS /,HEPATIC ENZYMES ANO TUMOR rilSTQPATHOLOGY 0 44- 2
ICTALJRUS MELAS (RAFINESQUE), LIVING IN A CHLORINATED SEWAG 15- 3
BURDENS ANO THE PREVALENCE OF PROLIFERATIVE DISORDERS IN MUSSELS (MYTI 31- 1
" IN THE BAY MUSSEL, MYTILUS EDULIS /,SEASONAL VARIATION IN SOFT 20- 4
ON CLEAR-CUT SITES /.PYROLYTIC DEPOSITION 0?= PDLYNUCLEAR AROMA 43- 1
PAGE 50
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KEYWORD TITLE INDEX p I
A T
G e
e M
c
CARCINOGEN ASSAY SYSTEM UTILIZING ESTUARINE FISHES /.DEVELOPMENT 0* A 22- 2
« /.GLUCURONSYL- AND SULFO- TRANS^ERASES IN FISH =XPOSED TO E 42- 1
» ASSAY^SLJBJECTS /.LABORATORY EVALUATION OF MARINE FISHES AS 9- 1
» EXPOSURE STUDIES /.PRIMARY CELL CULTURES FRCM THE TELFQST, 22- 1
" EXPOSED TELcQSTS: 3ROCEDURES FOR PREPARATION AND ANALYSIS 24- 1
CARCINOGENESIS IN FISH /.CHEMICAL 16- 2
M /.RAINBOW TROUT CSALMO GAIRDNERI) EMBRYOS: A SENSITIVE 18- 1
" TERATQGENESIS AND MUTAGENESIS / .USEFULNESS OF THE SELF 19- 1
CARCINOGENICITY TESTING CA FRIENDLY DISCUSSION/DEBATE 8Y DR. JOHN COUC 3- 2
" . •* TESTING: ADVANTAGES AND SPECIAL PROBLEMS /.USEFULNESS 13- 1
CARCINOGENS IN THE A1UATIC ENVIRONMENT /.AMERICAN QYSTCR CCRASSQSTREA 11- 1
" IN BIVALVE M3LLUSKS FROM OREGON ESTUARIES /.CHEMICAL 32- 1
" IN THE MARINE ENVIRONMENT. 3EMZ3CA5PYRENE IN ECONOMICALLY 33- 2
» MUTAGENS, AND TERiTOGENS ON NON-HUMAN SPECIES (AQUATIC AN 6- 2
« MUTAGENS AND TERATOGENS ON NON-HUMAN SPECIES-AQUATIC UNIM 7- 1
* MUTAGENS, AND TERATOGENS ON NONHUMAN SPECIES CAQUATIC ANI 5- 2
MUTAGENS, AND TERSTOGENS ON NON-HUMAN SPECIES CAQUATIC AN 6- 2
MUTAGENS, AND TERATOGENS OF NONHUMAN SPECIES-AQUATIC ANIM 4- 1
MUTAGENS, AND TERATOGENS DN NON-HUMAN SPECIES CAQUATIC AN 6~ 2
/.EXPOSURE OF A TELEOST CELL LINE TO SUSPECTED 18- 2
MUTAGENS, AND TERATDGENS 3N NON-HUMAN SPECIES CAQUATIC AN 14- 2
" /,SU?J'M$PY IF TISSUE LESIONS IN AQUATIC ANIMALS INDUCED BY 26- 3
CATFISH, AND GULF
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KEYWORD TITLE INDEX p I
A T
G E
E M
C
CtYDE DAWE) - DEBATE: THE FISHY SIDE /,MOUSE VS MINNOW: THE FUTURE 0 3-2
COISTAL WATERS —AM OVERVIEW /, 3IQMONITORING OF 10- 1
CQHO SALMON! REARED IN CHLORINATED-DECHLORINATED WATER /.LIMITED EPIZOQ 26- 1
« SALMON /.TC3RAMYCIN NEPHR3T3XICITY AND LETHALITY IN 38- 2
COLLABORATIVE aROGRA* /,EFFECTS OF CARCINOGENS, MUTAGENS, AND TERATOGE 5- 2
COWARATIVE B£NZO(A)PYRENE METABOLITE PATTERNS IN FISH AND RODENTS 24- 2
COMPLEX CH3QMATOPHOR3MA IN A MARINE TELEDST PISH, FUNOULUS GRAMDIS: M 3-1
COMPOUNDS ON E3TUARINE PISHES /.EFFECTS OF PETROLEUM 2*- 1
CONCENTRATING AMMONIA = RDM SMALL VOLUMES OF SEAW5TER /,ION-EXCHANGE TE 35- 3
CONCENTRATION ON ELECTRQ^HDRETIC PATTERNS 3F ADDUCTOR MUSCLE PROTEINS 41- 1
CONCENTRATIONS IN MUSSELS (MYTILUS EDULIS) FROM YAQUINA BAY, OREGON DU 31- 2
" IN SOMATIC AND GONAD TISSUES OF BAY MUSSELS, MYTILUS ED 28- 2
" °'~ UNSU3STITUTEO ^OLYNUCLJA^ AROMATIC HYDROCARBONS IN B 27- 2
M OF UNSUBSTITUTED POLYCYCLIC AROMATIC HYDROCARBONS IN SO 27- 1
CONCURRENT NEOPLASTIC AND PR3TISTAN DISORDERS IN THE AMERICAN OYSTER C 11- 2
CONDITIONS (ABSTRACT) /.ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE 30- 2
CONFIRMATION 0= METABOLITES OF SENZOCA)PYRrNc ^RQM A MARINE FISH (ABST 40- 3
CONTAMINANTS*AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL CONDITIONS (AB 30- 2
11 CHEMOTHERA^EUTIC AGENTS, AND POTENTIAL CARCINOGENS /.SUM 26- 3
CONTROLLED EXPOSURES TO ENVIRONMENTAL CONTAMINANTS, CHEMOTHERAPEUTIC A 26- 3
COOS*BAY, OREGON, USA /, CONCENTRATIONS DF UNSU3STITUTF. 3 POLYCYCLIC ARO 27- 1
CORRELATION OF MIX ED-F'JNCTIO.N QXIDASe ACTIVITY WITH ULTRASTRUCTURAL CH 40- 2
COTTONSEED OIL TO RAINBOW TROUT CSALMO GAI3DNERI) /.HEPATQCARCINQGENIC 17- 2
COTTONSEEDS AND REFINE!) COTTONSEED OIL TO RAINBOW TROUT CSALMO GAlRDNE 17- 2
COUCH AND DR. CLYDE DAME) - DEBATE: THE FISHY SIDE /,MOUSE VS MINNOWS 3- 2
CRASSOSTREA VIRGINICA) AS AN INDICATOR Or CARCINOGENS IN THE AQUATIC E 11- 1
" VIRGINICA /,3ENZ3(A)PYRENE METABOLISM IN THE AMERICAN 3YST 1- 2
H VIRGINICA) /.CONCURRENT NEOPLASTIC AND PROTISTAN DISORDERS 11- 2
" GIGAS. M.S. THESIS /.EFFECTS OF X- AND GAMMA IRRADIATION 47- 1
11 GIGAS) /,REGENERATION DF RADIATION-DAMAGED DIGESTIVE TISSU 46- 1
CRUSTACEA: PENAEICAS) /.SHRIMPS CARTHROPODA: 12- i
CULTURE ESTA3LISMENT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES /, 22~ 1
CULTURES FROM THE TELE3ST, CYPRIN030N VARIEGATUS: CULTURE ESTABLISMEN 22- 1
CUT SITES /.PYROLYTIC DEPOSITIIN op ^OLYMUCLEAR AROMATIC HYDROCARBONS 43- i
CYCLE INTERMEDIATES, LACTIC ACID, AND THE AMIN3 ACID TSURINE /,THIN-LA 37- 1
CYPRINODON VA^IEGATUS) (ABSTRACT) /,EM3RYOLOGIC DEVELOPMENT OF THE SHE 34- 1
11 VARIE3ATUS CA3STRACT) /.HISTOLOGY 0= THE POST-PHARYNGEAL 01 35- 1
" VARIEGATUS (ABSTRACT) /.M3RPHOLOGY OF PERIPHERAL BLOOD CELL 21- 2
" VA2IEGATUS: CULTURE 5STA3LISMENT AND APPLICATION IN CARCIN 22- 1
" VARIEGATUS AND FUNDULUS G3ANDTS IN C/J^CINOGENICITY TESTING: 13- 1
CYPRINODONTID ?ISH, RIVULUS MARMORATUS AS AN EXPERIMENTAL ANIMAL IN ST 19- 1
D
DAMAGED DIGESTIVE TISSUES IN JUVENILE PACIFIC 3YSTERS CCRASSOSTREA GIG 46- 1
DAKE) - DEBATE: THE FISHY SIDE /.MOUSE vs MINNOW: THE FUTURE OF FISH 3- 2
DEBATE: THE FISHY SIDE /.MOUSE VS MINNOW: THE FUTURE OF FISH IN CARC 3- 2
" BY DR. JOHN COUCH AND DR. CLYDE DAW5) - DEBATE: THE FISHY SIDE 3- 2
OECHLORINATED WATER /.LIMITED EPIZOOTIC DF NEUROBLASTOMA IN COHO'SALMO 26- 1
DEGREES (ABSTRACT) /.INDUCTION OF MFQ IN MULLET LIVER MICRQSOMES: EF 39- 2
" AND 37 DEGREES (ABSTRACT) /.INDUCTION 0= MFQ IN MULLET LIVER 39- 2
DEPOSITION OF POLYNUCL~Ai? AROMATIC HYDROCARBONS DUE TO SLASH BURNIMG 0 43- 1
DEPURATION, SIZE AND SEX ON TRACc METAL LEVELS IN BAY MUSSELS /.EFFECT 20- 1
DETECTION (ABSTRACT) /.METiBOLITES OF 8ENZO(A)'Y*ENE FROM MULLET (MUGI 40- 1
DETERMINATION DF 1,2-DIOLS BY INDIRECT ATOMIC ABSORPTION WITH DIGESTED 45- 3
" OF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EX 20- 3
DEVELOPMENT OF A CARCINOGEN ASSAY SYSTtM UTILIZING ESTUARINE FISHES 22- 2
" OF THE SHEEPSH'AO MINNOW (CYPRINODON VARIEGATUS) (ABSTRACT 34- 1
WETART EXPOSURE TO SENZD(A)PYRENE (ABSTRACT) /,LIVER NEOPLASIA AND IN 17- i
WFFERENTIAL ABILITY OF AMSYSTOMA TIGRINUM HEPATIC MICROSOMES FROM POL i- i
PAGE 52
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KEYWORD TITLS INDEX P I
A T
G 5
E M
0
DIGESTED LEAD PE5IODAT5 ^DETERMINATION OF 1,2-DICLS BY INDIRECT ATOHI 45- 3
DI6ESTIVE TRACT OF THE SHEEPSHEAD MINNOW, CYPRIN3D3N VARIEGATUS CA3STR 35- 1
TISSUES IN JUVENILE PACIFIC 3YSTERS CC3ASSOSTREA GIGAS) /,RE 46- 1
DI8LS BY INDIRECT ATOMIC A3SQRPTIQN WITH DIGESTED LEAD PERIODATE /,DET 45- 3
BISCUSSION/DESATt 3Y DR. JOHN COUCH ANO DR. CLYDE DAWE) - DEBATE: THE 3- I
DISEASES Or INVERTEBRATES OTHER THAN INSECTS /,VIRAL 9- 2
DISORDER IN OYSTERS COST3EA CHILENSIS) FROM CHILOE, CHILE, SOUTH AMERI 30- 1
DISORDERS IN MUSSELS CMYTILUS EDULIS) IN OREGON /, BENZO
-------
KEYWORD TITLC INDEX P I
A T
G E
E M
E
EVAPORATION /.SIMPLE GAS CHR1MATQGRAPHIC METHOD PQR TH= STUDY OF ORGAN 44- 1
EXCHANGE RESIN NEUTRON ACTIVATION /.DETERMINATION QP VANADIUM IN A MAR 20- 3
« TECHNIQUE FOR CONCENTRATING AMMONIA P2QM SMALL VOLUMES OF SEA 35- 3
EXPOSURES- A TELE3ST CELL LINE TO SUSPECTED CARCINOGENS 18- 2
« Cr FISHES TG 3E.MZ DC A)PYRE ME AND SOME ASPECTS DF ANALYSIS OF M 38- 3
» Or TW3 TELECST SPECIES TO POLYCYCLIC AROMATIC HYDROCARBONS CA 23- 2
• TO 3ENZO(A)PY^ENE (ABSTRACT) /.LIVER NEOPLASIA AND INDUCTION 17- 1
• STUDIES /.PRIMARY CELL CULTURES FROM THE TELE3ST, CYPRINODON 22- 1
EXPOSURES /.AQUATIC ANIMALS AS INDICATORS DF ENVIRONMENTAL 6~ 1
' " TO ENVIRONMENTAL CONTAMINANTS, CHEMQFHERAPEUTIC AGENTS, AND 26- 3
F
FERTILIZING CY^RINODONTIO FISH, 3IVULUS MARMORATUS AS AN EXPERIMENTAL 19- 1
FISH CELL LINE FROM A MALE SHEEPSHEAD /.ANALYSIS OF A MARINE 14- 3
« CELL LINES /, C-BANjDING OF CHROMOSOMES FROM THREE ESTABLISHED MARI 15- 1
• IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /.CAN TISSUE ANOMALIES TH 34- 2
" EXPOSED TO CHLORIMATE3 SEWAGE EFFLUENT /.CAUSES OF PAPILLOMAS ON 15- 2
« /.CHEMICAL CARCIN3GENESIS IN 16- 2
« AND RODENTS /,COMPARATIVE 2ENIO(A)PYRENF METABOLITE PATTERNS IN 24- 2
» FUNDULUS GRANDIS; MOPPHQLOGICAL AND BIOCHEMICAL CHARACTERISTICS 3- i
" /.CORRELATION OF MIXED-FUNCTION OXIOA5E ACTIVITY WITH ULTRASTRUCT 4Q- 2
" (ABSTRACT) /.EJECTS OF TRICHLORDPROPENE OXIDE ON THE METABOLISM 39- 1
" (ABSTRACT) /,FLUO^IMETR1C CONFIRMATION OF METABOLITES OF 3£NZO(A) 40- 3
" EXPOSED TO ENVIRONMENTAL CARCINOGEN /.GLUCURONSYL- AND SULF3- TRA 42- 1
" IN CARCINOGENICITY TESTING (A FRIENDLY DISCUSSION/DEBATE 8Y DR. J 3-2
• TO TRANS-STIL3ENE OXIDE TREATMENT /.RESPOMSES OF THE HEPATIC ENZY 45- 2
M /.SEROLOGIC ALTERATIONS IN CARCINOGEN-EXPOSED TELEQSTS: PROCEOUR 24- 1
H RIVULUS MARVORATUS AS AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVIN 19- 1
11 EXPOSED TO THE HERBICIDE TRIFLU^ALIN /.VERTEBRAL DYSPLASIA IN YOU 12- 2
FISHES /.DEVELOPMENT CF A CARCINOGEN ASSAY SYSTEM UTILIZING ESTUARINE 22- 2
";*•• /.EFFECTS OF PETROLEUM COMPOUNDS ON ESTUARINJE 23- 1
11 TO 3ENZO(A)PYRENE AND SOME ASPECTS OF ANALYSIS OF METABOLITES / 38- 3
" /.HISTOPATHOLOGY OF FOUR SPONTANEOUS NEOPLASMS IN THREE SPECIES 26- 2
tt AS CARCINOGEN ASSAY SUBJECTS /.LABORATORY EVALUATION OF MARINE 9- 1
FISHY SIDE /.MOUSE VS MINNOW: THE FUTURE OF FISH IN CARCINOGEMlCIfY T 3-2
FLOW THROUGH SYSTEM /.UPTAKE AND ACCUMULATION OF NAPHTHALENE BY THE OY 36- 1
FLMORIMETRIC.CONFIRMATION OF METABOLITES OF 8FMZQ(A}PYRENE FROM A MARI 40- 3
FRACTIONS OF ARQCLCR 1254-TREAT5D MULLET (MUGIL CEPHALUS) /.BENZOCAOPY 43- 3
FRIENDLY. DISCUSSIQN/OE3ATE BY DR, JOHN COUCH AND DR, CLYDE OAWE) - DEB 3- 2
FUNDULUS GRANDIS: MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS /.COM 3- 1
" GRANDIS IN CASCINOGENICITY TESTING: ADVANTAGES AND SPECIAL P 13- 1
FUTURE OF FISH IN CARCINOGENICITY TESTING (A FRIENDLY DISCUSSION/DEBAT 3- 2
G
GAIRONERI) /.H'PATOCARCINOGENICITY OF GLANDLESS COTTONSEEDS AND REFINE 17- 2
" EMBSYOS: A SENSITIVE ANIMAL MOOEL FOR EXPERIMENTAL CARCINO 18- 1
GAMMA IRRAOIATION ON THE JUVENILE PACIFIC OYSTER, CRASSOSTREA GIGAS, 47- 1
GAS CHROMAT03RAPHIC METHOD FOR THE STUDY OF ORGANIC SOLVENTS: MOISTUR 44- 1
GISAS. M.S. THESIS /,'FFtCTS OF X- AND GAMMA IRRADIATION ON THE JUVEN 47- 1
11 /.REGENERATION OF RADIATION-DAMAGED DIGESTIVE TISSUES IN JUVENI 46- 1
GLANDLESS CDTTONSE=DS AND REFINED COTTONSEED OIL TO RAINSOW TROUT
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KEYWORD TITLE INDEX P I
A T
G E
E M
G
6ULF KILLI-=ISH /.MIXED FUNCTION OXIDASE INDUCIBILITY AND POLYAROMATIC 25- 2
H
HJEMIC NEOPLASMS OF BAY MUSSELS, MYTILUS EDULIS, FROM OREGON: OCCURRE 27- 3
HENATOPOIETIC NEOPLASMS IN INVERTEBRATES AND COLD-FLOODED VERTEBRATES 16- 1
HEPATIC S-9 FRACTIONS OF AROCLOR 125*-TREATEO MULLET (MUGIL CEPHALUS) 43- 3
« MICROSOMES FROM POLYCYLIC AROMATIC HYDROCARBONS AND AROMATIC A 1- 1
" ENZYMES AND TUMOR HISTDPATHDLOGY QF 3LACK BULLHEADS WITH PAPIL 44- 2
MIXE3 FUNCTION OXIDASE ENZYMES THE RAINBOW TRCUT FOLLOWING DIE 17- 1
'« ENZYMtS OF A TEL50ST FISH TO TRANS-STILBENE OXIDE TREATMENT /, 45- 2
HEPATOCARCINOGENICITY QF GLANDLESS COTTONSEEDS AND REFINED COTTONSEED 17- 2
HERBICIDE TRIFLURALIN /,HISTDPATHDLOGY AND ENLARGEMENT OF THE PITUITAR 5- 1
" TRIFLURALIN /.VERTEBRAL DYSPLASIA IN YQUMG FISH EXPOSED TO T 12- 2
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY /.SEPARATION OF THE ISQMERIC PH 13- 2
« PERFORMANCE LIQUID CHRQMATOGRAPHY METHOD FOR SEPARATION OF SE^ZQC 14- 1
HISTOLOGY OF THE POST-PHARYNGEAL DIGESTIVE TRACT OF THE SHEEPSHEAO MIN 35- 1
HISTOPATHOLOGICAL PROGRESSION /tHAEMIC NEOPLASMS OF 3AY MUSSELS, MYTIL 27- 3
HISTOPATHOLOGY OF 3LACX BULLHEADS WITH PAPXLLQM4S /.HEPATIC ENZYMES AN 44- 2
" AND ENLARGEMENT OF THE PITUITARY OF A TELEOST EXPOSED T 5-1
" 0= FOUR SPONTANEOUS NEOPLASMS IM THREE SPECIES OF SALMO 26- 2
(WHAN SPECIES (A3UATIC ANIMALS) /,EFFECTS OF CARCINOGENS, MUTAGENS, AN 6- 2
" SPECIES-AQUATIC ANIMALS //EFFECTS OF CARCINOGENS; MUTAGENS AND T 7-1
" SPECIES (AQUATIC ANIMALS) /,EFFECTS OF CARCINOGENS. MUTAGENSf AN 6- 2
11 SPECIES CAQUATIC ANIMALS) /.INDEX AND ABSTRACTS TO PUBLICATIONS. 14- 2
HYDROCARBON OXIDASE IN KARINE ORGANISMS (ABSTRACT) /.INDUCTION OF POLY 25- 1
11 METABOLISM IN THE MULLET, SEA CATFISH, AND GUL= KILLIFISH 25- 2
HYDROCARBONS IM SAY MUSSELS CMYTILUS EDULIS) FROM OREGON, USA /.CONCEN 27- 2
" IN SOFTSHELL CLAMS FROM CCOS BAY, OREGON, USA /,CONCENTRA 27- 1
» AND AROMATIC AMINES /.DIFFERENTIAL ABILITY 3F AMBYSTOMA T 1- 1
M (ABSTRACT) /.EXPOSURE OF TWO TELEOST SPECIES TO POLYCYCLI 23- 2
" (ABSTRACT) /.INDUCTION OF GLUCURONIOE AND SULFATE TRANSFE 42- 2
" AND CELLULAR PROLIFSRATIVE DISORDERS IN BIVALVE .MOLLUSCS 29- 1
11 IN BIVALVE MOLLUSKS IN BAY MUSSELS (MYTILUS EDULIS) FROM 29- 2
" DUE TJ SLASH BURNING ON CLEAR-CUT SITES /.PYROLYTIC DEPOS 43- 1
" AMD METALS IN MOLLUSCS (ABSTRACT) /.STUDIES ON POLYNUCLEA 28- 1
HYDROLASE /.INDIRECT ATOMIC ABSORPTION SPECTRO^E-TRIC ASSAY FOR EPOXIOE 44- 3
HY68QSCOPICITY, AND EVAPORATION /.SIMPLE GAS CHROMATOGRSPHIC METHOD FQ 44- 1
I
ICTALURUS ME3ULOSIS. M.S. THESIS /.EFFECTS OF AFLATOXIN ON THE 3ROWM 2- 1
" MELAS (RAFINES-3UE), LIVING IN A CHLORINATED SEWAGE POND /,PA 15- 3
IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /.CAN TISSUE ANOMALIES THAT QC 34- 2
IMPORTANT 3IVALVE MOLLUSKS FROM OREGON ESTUARIES /.CHEMICAL CARCINOGEN 33- 2
MOEX AND ABSTRACTS TO PUBLICATIONS, THE EPA-NCI PROJECT: EFFECTS OF 14- 2
INOICATOR OF CARCINOGENS IN THE AQUATIC ENVIRONMENT /.AMERICAN OYSTER 11- 1
INDICATORS OF.ENVIRONMENTAL EXPOSURES /.AQUATIC ANIMALS AS 6- 1
INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAD PERIODATE /, DETERMINATE 45- 3
11 ATOMIC ABSORPTION S PECTROMETSIC ASSAY FOR EPOXIDE HYDROLASE 44- 3
INDUCED 3Y CONTROLLED =XPDSU??ES TO ENVIRONMENTAL CONTAMINANTS, CHEMOTH 26- 3
INDUCIBILITY AND POLYA30*ATIC HYDROCARBON METABOLISM IN THE MULLET, SE 25- 2
INDUCTION OF GLUCURDNIDE AMD SULFATE TRANSFERASES 3Y PHEN03ARBITAL AND 42- 2
" OF MFO IN MULL-T LIVER MICSOSOHES: EFFECT OF NADPH ON BENZ 39- 2
11 OF POLYAROMATIC HYOR1CAR30N OXIDISE IN MARINE ORGANISMS (A8S 25- 1
11 OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE RAINBOW TROUT 17- 1
INEXPENSIVE METHOD FOR MEASURING INTEGRATED LIGHT ENERGY /.SIMPLE AND 43- 2
INSECTS /.VIRAL DISEASES OF INVERTEBRATES OTH£S THAN 9- 2
JNTEGRATED LIGHT ENF3GY /,SIMPLE AND INEXPENSIVE METHOD =OR MEASURING 43- 2
JNTERMEDIATES, LACTIC ACID, AND THE AMINQ ACID TAURINE /.THIN-LAYER SE 37- 1
^VERTEBRATES flND COLD-BLOODED VERTEBRATES /,HEMATOPOIETIC NEOPLASMS I 16- 1
11 OTHER THAN INSECTS /,VIRAL DISEASES OF 9- 2
PAGE 55
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KEYWDRD TITLE INDEX P I
A T
G E
E M
I
flWOLYING CARCINQGENESIS, TERATCGENESIS ANO MUTAGENESIS /.USEFULNESS 0 19- 1
ION EXCHANGE RESIN NEUTRON ACTIVATION /.DETERMINATION 3? VANADIUM IN A 20- 3
H EXCHANGE TECHNIQUE FDR CONCENTRATING AMMONIA FROM SMALL VOLUMES OF 35- 3
IRRADIATION ON THE JUVENILE PACIFIC OYSTER, CRSSSOSTREA GIGAS. M.S. T 47- 1
ISOHERIC,PHENOLS 0? BENZ3CA)PYRENE BY HIGH-PERFQRMANCE LIQUID CHROMATQ 13- 2
J
JOHN'COUCH AND DR. CLYDE DiWE) - DEBATE: THE FISHY SIDE /.MOUSE VS MI 3- 2
JUVENILE PACIFIC OYSTER, CRASSQSTRrA GIGAS. M.S. THESIS /.EFFECTS OF 47- 1
" PACIFIC OYSTERS (CRASSOSTREA GIGAS) /.REGENERATION OF RADIATI 46- 1
K
KILLIFISH /.MIXED FUNCTION OXICA5E INDUCIBlLlTY AND PO^YAROMATIC HYDRO 25- 2
KIIETZC. PH.D. DISSERTATION /.EFFECTS OF CHEMICAL PERTURBATION 3Y NAP 38- 1
L
LABORATORY EVALUATION 3F MARINE DISHES AS CARCINOGEN ASSAY SUBJECTS 9- 1
LACTIC ACID, AND THE AMINQ ACID TAURINr /.THIN-LAYER SEPARATION OF ClT 37- 1
LA1VAL STAGES OF THE GOOSE BARNACLE, POLLICI?ES POLYMERUS COR MARINE B 33- 1
LATER SEPARATION 0= CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, AND 37- 1
LEAD PERICQATE /.DETERMINATION 0~ 1,2-OIOLS 3Y INDIRECT ATOMIC A8SORPT 45- 3
LESIONS IN AQUATIC ANIMALS INDUCED BY CONTROLLED EXPOSURES TO ENVIRONM 26- 3
LETHALITY IN CDHO SALM3N /.T38RAMYCIN NEPHROTCXICITY ANO 38- 2
LEVELS IN 3AY MUSSELS /.EFFECTS OF DEPURATION, SIZE AND SEX ON TRACE M 20- 1
» IN A POPULATION OF MYTILUS EDULIS PROM YAQUINA BAY, OREGON. .- PH 21- 1
LISHT ENERGY /.SIMPLE AND INEXPENSIVE METHOD FDR MEASURING INTEGRATED 43- 2
LIMITED EPIZCOTIC 3F NEUR03LASTOMA IN COHO SALMON REARED IN CHLORINATE 26- 1
LINE FROM A MALE SHEEPSHEAG /ANALYSIS C* A MARINE FISH CELL 14- 3
" TO SUSPECTED CARCINOGENS /.EXPOSURE Oc A TELFOST CELL 18- I
LIHES /.C-3ANDING ^ CHROMCSDMSS FRO^ THRES ESTA3LISHED MARINE FISH CE 15- 1
LIQUID CHRJMAT3G3APHY /.SEPARATION OF THE ISOMERIC PHENOLS OF BSNZO(A) 13- 2
" CHROMATQGRAPHY METHOD FOR SrPAR^TID^ OF SENZOCA)PYRENE META30LI 14- 1
LIVER DF A MARINE FISH /.CORRELATION OF MIXED-FUNCTION OXIDASE ACTIVIT 40- 2
" .S1ICROSOMHS: EFFECT OF NADPH ON BENZOC A )PYRENE META30LITE DISTR 39- 2
" NEOPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASS ENZYME 17- 1
LIVING IN A CHLORINATED SEWAGE POND /.PAPILLDMAS 0= BLACK BULLHEADS, I 15- 3
M
HALE SHEEPSH5AD /.ANALYSIS OF A MARINE FISH CELL LINE FROM A 14- 3
MARINE FISH CELL LINE CRQM A MALE SHEE°SHEAD /.ANALYSIS OF A 14- 3
" FISH CELL LINES /,C~BANDING Or CHROMOSOMES FROM THREE ESTABLISH 15- 1
• FISH IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /.CAN TISSUE ANOMA 34- 2
" ENVIRONMENT. 3ENZO
-------
METABOLITES
n
HETAL
n
KEYWORD TITLE INDEX
M
HETABOLISM IN AN OYSTER CC3TREA :DJLIS)-3ACTcRIA SYSTEM. M.S. THESIS
IN THE MULLET, SEA CATFISH, AND GUL^ KILLIFISH /.MIXED FUNC
METABOLITE PATTERNS IN FISH AND RODENTS /.COMPARATIVE 3ENZO(A)PYRENE
DISTRIBUTION AT 25 DEGREES AND 37 DEGREES (A8STRACT) /.IND
/•EXPOSURE OF FISHES TO BENZO(A)5YRENE AND SOME ASPECTS OF
OF BENZO(A)PYRENH PRQM A MARINE =ISH (ABSTRACT) /.FLUORIME
OF BSNZO(A)PYRENE FROM MULLET (MUGIL C5PHALUS): PRQPERTIE
OF BENZC(A>PYRSNE IN AROCLQR 1254 TREATED MULLET
(ABSTRACT) /.SIMPLE HIGH PERFORMANCE LIQUID CHROMATOGRAPH
LEVELS IN BAY MUSSELS /.EFFECTS OF D-PURftTION, SII5 AND SEX ON T
3URDENS IN THE BAY MUSSEL, MYTILUS EDULIS /.SEASONAL VARIATION I
• LEVELS IN A POPULATION OF MYTILUS EDULIS FROM YAQUINA BAY, OREGO
HETALS IN MQLLJSCS (ABSTRACT) /.STUDIES ON POLYNUCLEAR AROMATIC HYDROC
METHOD FOR MEASURING INTEGRATED LIGHT ENERGY /.SIMPLE AND INEXPENSIVE
• FOR THE STUDY OF ORGANIC SOLVENTS: MOISTURE ANALYSIS, HYGROSCQ
" FOR SEPARATION CF BENZC(A)PYRENE METABOLITES (ABSTRACT) /,SI«P
METHYLCHQLANTHRENE- AND PHEN3BARBITAL-TP=ATED MULLET (MUGIL CEPHALUS),
MFO IN MULLET LIVER MICROSOMES: EFFECT OF NADPH ON 8£NZO(A)PYRENE ME
HICROSOMES*FRCM PCLYCYLIC AROMATIC HYDROCARBONS AND AROMATIC AMINES /,
11 EFFECT CF NADPH CN 8ENZQ(A)PYRENE METABOLITE DISTRIBUTIO
MINNOW (CYPRINDDON VARIEGATUS) (ABSTRACT) /,EMBRYQLQGIC DEVELOPMENT OF
" CYPRINODON VARIEGATUS (ABSTRACT) /.HISTOLOGY 0= THE PQST-PHARY
CISH IN CARCINOGENICITY TESTING (A FRIENDLY DIS
ACTIVITY WITH ULTRASTRUCTURAL CHANGES IN THE LI
ENZYMES THE RAINBOW TROUT FOLLOWING DIETARY EXP
INDUCIBILITY AND »QLYAROMATIC HYDROCARBON METAB
CARCINOGENESIS /.RAIN8CW TROUT (SALMO GAIRDMERI
MOISTURE ANALYSIS, HYGRQSCOPICITY, AND EVAPORATION /.SIMPLE GAS CHROMA
MOLLUSC USING A CHELATING ION EXCHANGE RESIN NEUTRON ACTIVATION /,OET£
MOLLUSCS /.EFFECTS Oc SODIUM CHLORIDE CONCENTRATION ON ELECTROPHORETIC
" FROM OREGDN ESTUARIES /.POLYNUCLEAR AROMATIC HYDROCARBONS * AND
" (ABSTRACT) /.STUDIES ON POLYNUCLEAR AROMATIC HYDROCARBONS AND
FROM OREGON ESTUARIES /.CHEMICAL CARCINOGENS IN BIVALVE
FROM OREGON ESTUARIES /.CHEMICAL CARCINOGENS IN THE MARINE EN
11 IN 3AY MUSSELS (MYTILJS EDULIS) FROM OREGON /, POL YNUCLEAR ARO
MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS /.COMPLEX CHROMATOPHOROM
MORPHOLOGY OF PERIPHERAL BLOOD CELLS Oc CYPRINODON VARIEGATUS (ABSTRAC
MOUSE vs MINNOW: THE FUTURE OF FISH IN CARCINOGENICITY TESTING (A FRI
MUSIL CEPHALUS) /,3ENZ3CA3PYRENE METABOLISM IN HEPATIC S-9 FRACTIONS 0
" CEPHALUS), A MARINE FISH (ABSTRACT) /,ECFECTS OF TRICHLOROPROPEN
" CEPHALUS): PROPERTIES AND DETECTION (ABSTRACT) /.METABOLITES OF
MULLET (MUGIL CEPHALUS) /,3ENZQCAOPYRENE METABOLISM IN HEPATIC S-9 FRA
" (ABSTRACT) /.EF-ECT OF 2.3-OXIDE-3,3,3-TRICHLOROPROPANE ON BAP
" (MUGIL CEPHALUS), A MARINE =ISH (ABSTRACT) /.EFFECTS OF TRICHLO
" LIVER MICROSOMES: EFFECT oc NAOPH ON 3ENZO(A)PYRENE METABQLIT
* (MUGIL CEPHALUS): PROPERTIES AND DETECTION (ABSTRACT) /.METABO
" /.M-TABOLITES OF SENZO(A)PYRENE IN AROCLCR 1254 TREATED
SEA CATFISH, AND GULF XILLIFISH /.MIXED FUNCTION OXIDASE INDUC
PROTEINS FROM BIVALVE MOLLUSCS /.EFFECTS OF SODIUM CHLORIDE CON
MYTILUS EDULIS /.SEASONAL VARIATION IN SO-T TISSUE WEIGHTS AND
(MYTILUS EDULIS) IN OREGON /,BENZO(A)PYRENE 300Y BURDENS AND T
(MYTILUS EDULIS) FROM YA3UINA 3AY, OREGON DURING JUNE 1976 - M
MYTILUS EOULIS /,3CNZO(A)PYRENE CONCENTRATIONS IN SOMATIC AND
CDULIS) PROM OREGON, USA /.CONCENTRATIONS QF JNSU3STI
"iF DEPURATION, SIZE AND SEX ON TRACE METAL LEVELS IN
FROM OREGON: OCCURRENCE, PREVALENCE, SEASONA
FRDM YAQUINA SAY, OREGON /.OBSERVATIONS ON TH
FROM OREGON /.POLYNUCLEAR AROMATIC HYDROCARBO
PAGE 57
" THE FUTURE OF
MIXED-FUNCTION 'OXIDASE
11 FUNCTION OXIDASE
" FUNCTION OXIDASE
MODEL FO.R EXPERIMENTAL
MOLLUSKS
n
MUSCLE
HUSSEL,
MUSSELS
n
(MYTILUS
/,EFFECTS
MYTILUS EDULIS,
MYTILUS EDULIS,
(MYTILUS EDULIS)
P I
A T
G E
E M
2- 2
25- 2
24- 2
39- 2
38- 3
40- 3
40- 1
45- 1
14- 1
20- 1
20- 4
21- 1
28- 1
43- 2
44- 1
14- 1
39- 1
39- 2
1- 1
39- 2
34- 1
35- 1
3- 2
40- 2
17- 1
25- 2
18- 1
44- 1
20- 3
41- 1
29- 1
28- 1
32- 1
33- 2
29- 2
3- 1
21- 2
3- 2
43- 3
39- 1
40- 1
43- 3
41-2
39- 1
39- 2
40- 1
45- 1
25- 2
41- 1
20- 4
31- 1
31- 2
28- 2
27- 2
20- 1
27- 3
32- 2
29- 2
-------
KEYWORD TITLE INDEX P I
A T
G e
E H
M
NUSSELS CMYTILUS EOULIS) /,SEASONAL VARIATIONS OF ARSENIC AND OTHER TR 20- 2
KUTAGENESIS /.USEFULNESS OF THE SELF-FERTILIZING CYPRINOOONTID FISH, R 19- 1
NUTA6ENS, AND TERATOGENS ON NON-HUMAN SPECIES (AQUATIC ANIMALS) /.EFFE 6- 2
» AND TERATOGENS ON NON-HUMAN SPECIES-AQUATIC ANIMALS /.EFFECTS 7- 1
« *ND TERATOSENS ON NCNHUMAN SPECIES (AQUATIC ANIMALS): FOURT 5- I
AND TESATOGENS DM NON-HUMAN SPECIES (AQUATIC ANIMALS) /.EFFE 6- 2
AND TERATOGENJS 0= NQNH'JMAN SPECIES-AQUATIC ANIMALS (ABSTRACT 4- 1
«« AND TE3ATOG£MS CN NON-HUMAN SPECIES (AQUATIC ANIMALS) /,5FFE 6- 2
AND TE3ATOGEMS ON NON-HUMAN SPECIES (AQUATIC ANIMALS) /.INDE 14- 2
NTTILUS EDULIS) IN CRESON /,BENZO(A)PYRENE BODY 3URD5NS AND TH* PREVAL 31- 1
« EDJLIS) -RIM YAQUINA BAY, OREGON DURING JUNE 1976 - MAY 1978 / 31- 2
" EDJLIS /tBENZQCA)PYRENE CONCENTRATIONS IM SOMATIC AND GONAD TI 28- 2
" ED'JLIS) FROM OREGON, USA /.CONCENTRATIONS OF UNSU8STITUTED POL 27- 2
» EDULIS, FROM OREGON: OCCURRENCE, PREVALENCE, SEASONALLY,' AND 27- 3
» ED'JLIS, FROM YAQUINA BAY, OREGON /.OBSERVATIONS ON THE ULTRAST 32- 2
« EDULIS) FROM OREGON /,POLYNUCLrAR AROMATIC HYOROCARBONS IN 8IV 29- 2
" EDJLIS./.SEASONAL VARIATION IN SOFT TISSUE WEIGHTS AND TRACE M 20- 4
11 EDULIS) /.SEASONAL VARIATIONS OF ARSENIC AND OTHER TRACE ELEME 20- 2
« EDULIS FROM YAQUINA BAY, "OREGON. PH.D. THESIS /.TRACE METAL L 21- 1
N
NAOPH 3N 3ENZO(A)?YRENE METABOLITE DISTRIBUTION AT 25 DEGRESS AND 37 39- 2
NAPHTHALENE ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER COSTREA 38- 1
M ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER, OSTREA 35- 2
" ON GLUCOSE TRANSPORT IN THE OYSTER (ABSTRACT) /.STIMULATOR 37- 2
* 3Y THE OYSTER OSTREA EOULIS, IN A -LOW THROUGH SYSTEM /.UP 36- 1
NEBULOSIS. M.S. THESIS /.EFFECTS OF Af=L4TOXIN ON THE SRDWN BULLHEAD I 2-1
MEQPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE 17- 1
NEOPLASMS OF BAY MUSSELS. MYTILUS EDULIS. FROM OREGON: OCCURRENCE, PR 27- 3
11 IN INVERTEBRATES AND COLD-BLOODED VERTEBRATES /, HEMATQPQIETI 16- 1
n IN THREE SPECIES OF SALMONID FISHES / .HISTOPATHOLOSY OF FOUR 26- 2
MEOPLASTIC AND PRQTISTAN DISORDERS IN THE AMERICAN OYSTER (CRASSQSTREA 11- 2
11 DISORDERS OF MUSSELS, MYTILUS EDULIS, F3QM YAQUINA BAY, ORE 32- 2
NEPHROTOXICITT.AND LETHALITY IN COHQ SALMON /.TOBRAMYCIN 38- 2
NEUSOBLASTQHA IN COHC SALMON REARED IN CHLORINATED-DECHLQRINATED WATER 26- 1
NEUTRON ACTIVATION /.DETERMINATION Op VANADIUM IN A MARINE MOLLUSC USI 20- 3
WO«HUHAN SPECIES (AQUATIC ANIMALS): FOURTH ANML'AL REPORT NCI/EPA COLL 5- 2
" SPECIES-AQUATIC ANIMALS (ABSTRACT) /.EFFECTS OF CARCINCGEMS, *- 1
0
OBSERVATIONS'ON THE ULTRASTRUCTU^E OF LARGE CELLS ASSOCIATED WITH PUTA 32- 2
OCCU8 IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT CHEMICALS? /.CAN TIS 34- 2
OCCURRENCE 0? CERTAIN PATHOLOGICAL CONDITIONS (ABSTRACT) /.ENVIRONMENT 30- 2
11 PREVALENCE, SEASONALITY, AND HISTO^ATHOLOGICAL PROGRESSION 27- 3
OIL TO RAINBOW T^OUT (SALMO GAIRDNERI) /.HEPAT3CARCIN03ENICITY OF GLAN 17- 2
ORGANIC SOLVENTS: MOISTURE ANALYSIS. HYGROSCOPICITY. AND EVAPORATION 44- i
ORGANISMS (ABSTRACT) /.INDUCTION OF POLYAROMATIC HYDROCARBON OXIDASE I 25- 1
OSTREA CHILENSIS) FROM CHILOE, CHILE, SOUTH AMERICA /.CELLULAR PROLIFE 30- 1
" PDULIS): AN IN VIVO KINETIC. PH.D. DISSERTATION /.EFFECTS OF C 38- 1
• EDULIS /,=FPECTS OF NAPHTHALENE ON GLUCOSE METABOLISM IN THE EU 35- 2
* EOULISJ-BACTERIA SYSTEM, M.S. THESIS /.EVALUATION OF BENZOCA)P 2- 2
" EDULIS, IN A FLOW THROUGH SYSTEM /.UPTAKE AND ACCUMULATION OF N 36- 1
OVERVIEW /.3IOMONITORIMG OF COASTAL WATERS —&N 10- 1
OXIDASE ACTIVITY WITH ULTRASTRUCTURAL CHANGES IN THE LIVER QP A MARINE 40- 2
" IN MARINE ORGANISMS CASSTRACT) /.INDUCTION OF PQLYARQMATIC HYD 25- 1
11 ENZYMES TH= RAINBOW TROUT FOLLOWING DIETARY EXPOSURE T3 B£NZO( 17- 1
* INOUCISILITY AND POLYAROMATIC HYDROCARBON META30LISM*IN THE MU 25- 2
&XlDE-3,3,3-TRICHLORDP*OPANE ON SAP METABOLISM IN MULLET (ABSTRACT) /, 41- 2
" ON THE M-T*80LISM OF B6NZO(A)PYR3NE 3Y 3-METHYLCHOLANTHRENE- AND 39- 1
* TREATMENT /.RESPONSES OF THE HEP&TTC ENZYMES OF A TELEOST FISH T 45- 2
PAGE 53
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KEYWORD TITLE INDEX P I
A T
G E
E M
0
8TSTER CCRASSOSTREA VIRGINICA) AS AN INDICATOR 0~ CARCINOGCNS IN THE A 11- 1
« CRASSOSTREA VIRGINICA /,B?NZO(A)PYR~NE METABOLISM IM THE AMERIC 1- 2
CCRASSCSTREA VIRGINICA) /.CONCURRENT NEDPLASTIC AND PROTISTAN D 11- 2
(QSTREA EDULIS): AN IN VIVO KINETIC. PH.D. DISSERTATION /.EFFE 38- 1
« OSTRcA EDULIS /.EFFECTS OF NAPHTHALENE OM GLUCOS= METABOLISM I 35- 2
» CR4SSOST3EA GIGAS. M.S. THESIS /.EFFECTS OF X- AND GAKMA IRRA 47- 1
COSTREA EDULIS)-SACTERIA SYSTEM, M.S. THESIS /.EVALUATION OF B 2-2
« (ABSTRACT) /.STIMULATORY EFFECT OF NAPHTHALENE ON GLUCOSE TRANS 37- 2
« OSTREA EDULIS, IN A FLOW THROUGH SYSTEM /.UPTAKE AND ACCUMULATI 36- 1
OYSTERS COSTREA CHILENSIS) FROM CHILOE, CHILE, SOUTH AMERICA /.CELLULA 30- 1
" (CRASSQSTREA GIGAS) /iREGENERATION OF RADIATION-DAMAGED DIGEST 46- 1
P
PACIFIC OYSTER, CRASSCSTREA GIGAS. M.S. THESIS /,EFFECTS OF'X- AND GA 47- 1
" OYSTERS (CRASSOSTREA GIGAS) /.REGENERATION OF RADIATION-DAMAGE 46- 1
PAPILLOMAS ON FISH EXPOSED TO CHLORINATED SEWAGE EFFLUENT /.CAUSES OF 15- 2
" /,HEPATIC ENZYMES AND TUMOR hISTQPATHOL3GY DF BLACK 3ULLHEA 44- 2
" OF BLACK 3ULLHEADS, ICTALURUS HELAS (RAFINESQUE), LIVING IN 15- 3
PATHQBIOLOSICAL MEASURES OF MARIME POLLUTION EFFECTS 18- 3
PATHOLQGICALtCONDITIONS CABSTRACT) /,ENVIRONMENTAL CONTAMINANTS AND TH 30- 2
PATTERNS-IN FISH AND.RODENTS /.COMPARATIVE BENZO(A)PYRENE META80LITE 24- 2
" OF ADDUCTOR MJSCLE PROTEINS FROM BIVALVE MOLLUSCS /.EFFECTS 0 41- 1
PEMAEIDAE) /.S-iRIM'S CAPTHROPODA: CRUSTACEA: 12- 1
PERFORMANCE LIQUID CHROMATQGRAPHY /.SEPARATION OF THE ISQMERIC PHENOLS 13- 2
" LIQUID CHROMATOGRAPHY METHOD FOR SEPARATION OF BENZD
-------
KEYWORD TITL5 INDEX P I
A T
G E
£ M
P
PROBLEMS /.USEFULNESS DP CYPRINQ3CN VARIrG^TUS AND FUNDULUS GRANDIS IN 13- 1
PROCEDURES FOR PREPARATION AND ANALYSIS OF SAMPLES FROM SMALL FISH /,S 24- 1
PROGRESSION /.HASMIC NEOPLASMS OF SAY MUSSELS, MYTILUS EOULIS, FROM OR 27- 3
PROLIFERATIVE DISORDERS IN MUSSELS CMYTILUS SDULIS) IN OREGON /,3ENZO< 31- 1
" DISORDER IN OYSTERS COSTREA CHILSNSIS) FROM CHILQE, CHIL 30- 1
" DISORDERS IN BIVALVE MOLLUSCS FROM OREGON ESTUARIES /,PO 29- 1
PROPERTIES AND DETECTION (ABSTRACT) /,METABOLITES 0? BENZDCA)PYRENE FR 40- 1
PROTEINS FROM BIVALVE MOLLUSCS /,EFFECTS OF SODIUM CHLORIDE CONCENTRAT 41- 1
PROTISTAN DISORDERS IN THE AMERICAN OYSTER PYRESE BY HIGH-PERFQRMAN 13- 2
" OF 3E.MZ3CA>?>Y'?ENE METABOLITrS (ABSTRACT) /.SIMPLE HIGH PER 14- 1
" OF CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, AND THE AM 37- 1
P4GE 60
-------
KEYWORD TITL= INDEX P I
A T
G E
E M
S
SEROLOGIC ALTERATIONS IN CARCINOGEN-EXPOSED TELEQSTS: PROCEDURES FOR 24- 1
SEWGE EFFLUENT /,CAUSES 0* PA^ILLOMiS ON FISH EXPOSED T3 CHLORINATED 15- 2
POND /.PAPILLOMAS Or 3LACK BULLHEADS, I3TALURUS McLAS (RAFINESQ 15- 3
SEX ON TRACE M^TAL LEVELS IN SAY MUSSELS /,ECF5CTS OF DEPURATIDN, SIZE 20- 1
SHEEPSHEAD /.ANALYSIS 3F A MARINE FISH CELL LIME FROM A MALE 14- 3
" MINNOW (CYPRTMOOQN VA3ItGATUS) (ABSTRACT) /, EMBRYQLOGIC DEV 34- 1
" MINNOW, CY^IMODQN VARIEGATUS (ABSTRACT) /.HISTOLOGY OF THE 35- 1
SHRIMPS (ARTHRDP3DA: CRUSTACEA: :=>£NA;IDA£) 12- 1
SIDE /,MOUSE VS MINNOW: THE FUTURE 3F FISH IN CARCINCGENICITY TESTING 3- 2
SITES /.PYROLYTIC DEPOSITION OF POLYNUCLEAR AR3MATIC HYDROCARBONS DUE 43- 1
SIZE.AND SEXTON TRACE METAL LEVELS IN 3AY MUSSELS /,EFFECTS OF DEPURAT 20- 1
SUSH BURNING ON CLEAR-CUT SITES /,PYRQLYTIC DEPOSITION OF POLYNUCLEAR 43- 1
SODIUM CHLORIDE CONCENTRATION ON ELECTROPHQRETIC PATTERNS OF ADDUCTOR 41- 1
SOFT TISSUE WEIGHTS 4N3 TRACE METAL BURDENS IN THE BAY MUSSEL, MYTILUS 20- 4
SOFTSHELL CLAWS FR3M COOS 3AY, OREGON, USA /,CONCENTRATIONS OF UNSUBST 27- 1
SOLVENTS: MOISTURE ANALYSIS, HYGRCSCOPICITY, AND EVAPIRATION /,SIMPLE 44- i
SOMATIC AND GONAO TISSUES OF BAY MUSSELS, MYTILUS EOULIS /,8ENZO(A)PYR 28- 2
SPECIAL PR3BLEMS /,USEFULNESS QF CYPRINODON VARIEGATUS AND FUNDULUS GR 13- 1
SPECIES (AQUATIC ANIMALS) /,EFFECTS OF CARCINOGENS. MUTAGENS, AND TERA 6- 2
11 AQUATIC ANIMALS /,EFFECTS OF CARCINOGENS. MUTAGENS AND TERATOG 7- 1
» (AQUATIC ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE 5- 2
" CA3UATZC ANIMALS) /,EFFECTS OF CARCINOGENS, HUTAGEMS, AND TERA 6- 2
" AQUATIC ANIMALS CA3STRACT) /,EFFECTS 0= CARCINOGENS, MUTAGENS, 4- 1
11 (A3UATIC ANIMALS) /, EFFECTS OF CARCINOGENS, MUTAGENS, ANO TERA 6- 2
M TO PDLYCYCLIC AROMATIC HYDROCARBONS CA3STRACT) /,EXPOSURE QF T 23- 2
" OF SALMONID DISHES /,HISTQPATHQLQGY OF FOUR SPONTANEOUS NEOPLA 26- 2
H CA3UATIC ANIMALS) /,INDEX ANO ABSTRACTS TO PUBLICATIONS, THE E 14- 2
SPECIFIC POLLUTANT CHEMICALS? /,CAN TISSUE ANOMALIES THAT OCCUR IN MAR 34- 2
SPECTROMETRIC ASSAY FOR EP3XIDE HYDR3L4SE /,INDIRECT ATOMIC ABSORPTION 44- 3
SPONTANEOUS NEOPLASMS IN THREE SPECIES OF SALMONID FISHES /,'HISTDPATHO 26- 2
STAGES OF THc SOOSE BARNACLE, »DLLICIP€S PQLYMERUS FDR MARINE 3IOASSAY 33- 1
STILBENE OXIDE TREATMENT /,RESPONSES OF THE HEPATIC ENZYMES OF A TELEO 45- 2
STIMULATORY E = "ECT Of NAPHTHALENE DN GLUCOSE TRAiMS^ORT IN THE 3YSTER C 37- 2
SULFATE TRANSFERASES BY PH-^NOBARBITAL AND PQLYCYCLIC AROMATIC HYDROCA 42- 2
SULF8- TRA'-JS-ERASES IN FISH EXPOSED TO ENVIRONMENTAL CARCINOGEN /,GLUC 42- 1
SUSPECTED CARCINOGENS /,EXPOSURE OF A TELEQST CELL LINE TO 18- 2
T
TAUBINE /,THIN-LAYER SEPARATION 3F CITRIC ACID CYCLE INTERMEDIATES, LA 37- 1
TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES Or SEAWATER /,I 35- 3
TEiEOST FISH, rUXDULUS GRANDIS: MDRPHQLDGICAL AND BIOCHEMICAL CHARACT 3- 1
" CELL LINE TO SUSPECTED CARCINOGENS /.EXPOSURE DF A 18- 2
" SPECIES TO POLYCYCLIC AR3MATIC HYDROCARBONS (ABSTRACT) /.EXPOS 23- 2
11 =X?CSED TO THE H6R3ICIDE TRIFLURALIN /.HISTOPATHDLOGY AND ENLA 5- 1
" CYPRINODOM VARIEGATUS: CULTURE ESTABLISMENT AND APPLICATION 22- i
11 FISH TO TRANS-STIL3ENE OXIDE TREATMENT /.RESPONSES OF THE HEPA 45- 2
TELEOSTS: PROC^DU'ES =OR PREPARATION ANO ANALYSIS OF SAMPLES FROM SMA 24- 1
TERATOGENESIS AND MUTA5ENESIS /.USEFULNESS OF THE SELF-FERTILIZING CYP 19- 1
TERATOGENS CN NON-HUMAM SPECIES (AQUATIC ANIMALS) /.EFFECTS OF CARCINQ 6- 2
" ON NON-HUMAN SP-CIES-AQUATIC ANIMALS /.EFFECTS DF CARCI^OGE 7- 1
" ON NONHJMAN S°FCIES (AQUATIC ANIMALS): FOURTH ANNUAL REPOR 5- 2
" ON NON-HUMAM SPECIES CA3UATIC ANIMALS) /.EFFECTS OF CARCINO 6- 2
11 Or NOHHUMAN SPECIES-AQUATIC ANIMALS (ABSTRACT) /.EFFECTS OF 4- 1
" ON NON-rtUMAM SPECIES (AQUATIC ANIMALS) /.EFFECTS OF CARCINO 6- 2
" ON NON-HUMAN SP'ECIES (AQUATIC ANIMALS) /, INDEX AND ABSTRACT 14- 2
TESTING (A FSI^NDLY QTSCUSSION/DEBATE BY DR. JOHN COUCH AND DR. CLYDE 3- 2
" ADVANTAGES AND SPECIAL P303LEMS /.USEFULNESS OF CYPRINQDON V 13- 1
THESIS /.EFFECTS OF AFLATOXINJ ON THE B30WN 3ULLHEAD ICTALURUS NESULOSI 2- 1
* /.EFFECTS OF X- AND GAMMA IRRADIATION ON THE JUVENILE PACIFIC 0 4?- 1
PAGE 61
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KEYWORD TITLE INDEX p I
A T
G E
E M
T
THESIS /.EVALUATION OF BENZO(A)PYR:NE METABOLISM IN AN OYSTER (QSTREA 2- 2
» /.TRACE METAL LEVELS IN A POPULATION) OF MYTILUS -DULIS FROM YAQ 21- 1
THIN-LAYER SEPARATION 3F CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, 37- 1
TISRINUM HEPATIC MICRDSOMES FROM P3LYCYLIC AROMATIC HYDROCARBONS AND A 1-1
TISSUE ANOMALIES THAT 3CCUR IN MARINE rISH IMPLICATE SPECIFIC POLL'JTftN 34- 2
« WcIGHTS AND TRACE METAL 5JRDENS IN THE SAY MUSSEL, MYTILUS EDUL 20- 4
• LESIONS IN AQUATIC ANIMALS INDUCED 3Y CONTROLLED EXPOSURES TO E 26- 3
TISSUES OF BAY MUSSELS, MYTILUS EDULIS /,BENZO(A)PYREN= CONCENTRATIONS 28- 2
» IN JUVENILE PACIFIC OYSTERS CCRASSOSTR1-A GIGAS) /.REGENERATION 46- 1
TOBRAHYCIN NEPHROTOXICITY AND LETHALITY IN CQH3 SALMON 38- 2
TOXICITY (ABSTRACT) /.3ENZIDINE 22- 3
TRACE METAL LEVELS IN BAY MUSSELS /.EFFECTS CF DEPURATION, SIZE AND SE 20- 1
« METAL BURDENS IN THE BAY MUSSEL, MYTILUS EDULIS /.SEASONAL VARIA 20- 4
" ELEMENTS IN 3AY MUSStLS (MYTILUS EDULIS) /,SEASONAL VARIATIONS 0 20- 2
» METAL LEVELS IN A POPULATION OF .1YTILU3 =OULIS FROM YAQUINA SAY, 21- 1
TRACT OF THE SHEEPSHEA3 MINNOW, CYPRINODGN VARIEGATUS (ABSTRACT) /,HIS 35- 1
TRANS-STIL3ENH OXIDE TREATMENT /,RESPONSES OF THE HEPATIC SNZYMES OF A 45- 2
TRANSFERASES IN FISH EXPOSED T3 ENVIRONMENTAL CARCINOGEN /.GLUCURQNSYL 42- 1
» BY PHFN3BAR8ITAL AN3 POLYCYCLIC AROMATIC HYDROCARBONS CA 42- 2
TRANSPORT IN THE- OYSTER (ABSTRACT) /.STIMULATORY EFFcCT OF NAPHTHALENE 37- 2
TREATED MULLET (MU3IL Cg^HALUS) /, SENZ3CA3PYRENE METABOLISM IN HEPATIC 43- 3
* MULLET (MUGIL CEPHALUS), A MARINE FISH (ABSTRACT) /.EFFECTS OF 39- 1
" MULLET /.HETAB3LIT5S OF Bt*JZ3< A)P YRENE IN AROCLOR 1254 45- 1
TREATMENT /.RESP3NSES OF THE HEPATIC ENZYMES OF A TELEOST FISH TO TRAN 45- 2
TRICHLOROPROPANE 0\ 3AP META30LISM IN /ULLrT (ABSTRACT) /.EFFECT OF 2, 41- 2
TRICHLOROPROPENE QXICE ON THE METABOLISM OF 3ENZD(A)PYRENE 3Y 3-METHYL 39- 1
TRIFLURALIN /.HISTDFATHOLOGY AND ENLARGEMENT OF THE PITUITARY OF A TEL 5- I
" /.VERTrB^AL 3YSPLASIA IN YOUNG FISH! EXPOSED TO THE H?R8ICI 12- 2
TROUT (SAmO GAIRDNE?!) /.HEPATOCARCINOGENICITY 3F GLANDLESS COTTONSEE 17- 2
n FOLLDWING DIETARY EXPOSURE T3 R£NZO
-------
KEYWORD TITLE INDEX ? I
A T
G E
5 M
W
/.LIMITED EPIZOOTIC IF NEUR03L4STCMA IN CQHO SALMON REARED IN CH 26- 1
HATERS--AN OVERVIEW /, 313M3NITQRINS OF COASTAL 10- 1
WEIGHTS AND TRACE METAL 3URD5NS IN THE BAY MUSSEL, MYTILUS EOULIS /,SE 20- 4
Y
3fiY, OREGON CUBING JUNE 1976 - MAY 1973 /, 3ENZ!XA)PYRENE CONCE 31- 2
SAY, OREGON /,DBSERVATIOMS ON THE ULTRaSTRUCTURE DF LARGE CELL 32- 2
« BAY, ORESON. PH.D. THESIS /,TRACE METAL LEVELS IN A PQPULATIQ 21- 1
fQUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN /,VERTEBRAL DYSPLASIA 12- 2
PAGE 63
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AUTHOR INDEX
PAGE 64
-------
AUTH02 INDEX
A
IktlQTT* D.T. , JOINT AUTHOR.
PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARVAL
STAGES OF THE GOOSE BARNACLE, POLLICIPES PQLYM = RLiS *=OR MARINE
3IOASSAYS/,33-l
ABEL, DANIEL C., JOINT AUTHOR.
USEFULNESS OF THE SELF-FERTILIZING CYPRINODOMTID FISH, RIVULUS MARMQRATUS AS
AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING CARCINOGENESIS, TERATQGENESIS
ftND MUTASENESIS/,19-1
ANDERSON, ROBERT S.
BEN20CA)?YRENE METABOLISM IN THE AMERICAN QYSTSR CRASSOSTREA VIRGlNICA/,1-2
DIFFERENTIAL ABILITY OF AMBYSTOMA TIGRINSJM HEPATIC MICROSOMES FROM POLYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES/,1-1
AULL, J.L.i JOINT AUTHOR.
INDUCTION OF GLUCURONIOE AND SULFATE TRANSFE3ASES BY PHENQ3ARBITAL AND
POLYCYCLIC ARCMATIC HYDROCARBONS CABSTRACT)/,42-2
8
BENNETT, CASEY W., JOINT AUTHOR.
DETERMINATION OF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EXCHANGE
RESIN ME'JTRQN ACTIVATION/, 20-3
BIBA, OIANE MARY
EFFECTS OF A=LATOXIN ON THE 2ROWN BULLHEAD ICTALURUS NE8ULOSIS. M.S.
THESIS/,2-1
3REESE, WILBUR P.* JOINT AUTHOR.
CELLULAR PROLIFE2ATIVE DISORDER IN OYSTERS OSTREA CHILENSIS> FROM CHILOE,
CHILE, SOUTH AMERICA/,30-1
BUNTING,'DIANE L.
EVALUATION OF BE^JZOC A )P YRENE METABOLISM IN AN OYSTER COSTREA
EDULIS)-3ACTERIA SYSTEM. M.S. THESIS/,2-2
BUNTING, OIANE L., JOINT AUTHOR.
PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARVAL
STAGES OF THE GOOSE 3A?N^CLE, POLLICIPSS POLYM-RUS FOR MARINE
BIQASSAYS/,33-1
UPTAKE AND ACCUMULATION OF NAPHTHALENE BY THE OYSTER OSTREA EDULIS, IN'A
FLOk THROUGH SYSTEM/,36-1
C
CONSTANTINE, G.H., JOINT AUTHOR.
TOBRAMYCIN NEPHRITOXICITY AND LETHALITY IN COHO SALMON/,38-2
COUCH, JOHN A.
AMERICAN OYSTER CCRASSQSTRcA VIR3INICA) *S AM INDICATOR OF CARCINOGENS IN
THE AQUATIC ENVIRONMENT/,11-1
AQUATIC ANIMALS AS INDICATORS OF ENVIRONMENTAL EXPOSURES/,6-1
3IOMONITORING OF COASTAL WATERS--AN OVERVIEW/,10-1
PAGE 65
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AUTHOR INDEX
C
COUCH, JOHN A.
COMPLEX CHRDMATQPHDR3MA IN A MARINE TELEQST =ISH, =U«JOULUS GRANDIS:
MORPHOLOGICAL AND 3IDCHEMICAL CHARACTERISTICS/,3-1
CONCURRENT NEOPLASTIC ANO PRQTISTAN DISORDERS IN THE AMERICAN OYSTER
(CRASSQSTREA VIRGINICA)/,!1-2
EFFECTS OF CARCINCGENSt MUTAGENS AND TERATOGtNS ON NON-HUMAN SPECIES-AQUATIC
ANIMALS/.7-1
EFFECTS 3F CARCINOGENS, MUTA3ENS, AND TERATOSENS OP MONHUMAN SPECIES-AQUATIC
ANIMALS (ABSTRACT)/,4-1
EFFECTS OF CARCINOGENS, MUTAGENS, AND TE^ATQSENS ON NON-HUMAN SPECIES
(AQUATIC ANIMALS)/,6-2
EFFECTS OF CARCINOGENS, MUTAGEMS, AND TE3ATQGENS ON NON-HUMAN SPECIES
(AQUATIC ANIMALS)/,8-1
EFFECTS DF CARCINOGENS, MUTAGEMS, AND TERATOGENS ON NON-HUMAN SPECIES
(AQUATIC ANI'1ALS>/,10-2
EFFECTS 3F CARCINOGENS, MUTAG^NS, AND TERATOGENS ON NONHUMAN SPECIES
(AQUATIC ANIMALS): PQJRTH ANNUAL REPORT NCI/EPA COLLABORATIVE'PROGRAM/,5-2
HISTQPAT.-10LOGY AND ENLARGEMENT OF THc PITUITARY DF A TEL'OST EXPOSED TO THE
HERBICIDE TRIFLURALIN/,5-1
LABORATORY EVALUATION 3F MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS/,9-1
MOUSE vs MINNOW: THE FUTURE OF FISH IN CASCINQGENICITY TESTING CA FRIENDLY
DISCUSSION/DEBATE BY DR. JOHN COUCH AND OR. CLYDE DAWE) - OE3ATE: THE FISHY
SIDE/,3-2
SHRIMPS CARTHRQPDDA: CRUSTACEA: p5NAriDA€)/,i2-i
VERTEBRAL DYSPLASIA IN YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN/,12-2
VIRAL DISEASES C? INVERTEBRATES OTHER THAN INStCTS/,9-2
COUCH, JOHN A.I JOINT AUTHOR.
CAN TISSUE ANOMALIES THAT OCCUR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT
CHEMICALS?/,34-2
CORRELATION OF MIXED-FUNCTION QXIDASS ACTIVITY WITH ULTRASTRUCTURAL CHANGES
IN THE LIVER Cc A MARINE FISH/,40-2
HEMATOPOIETIC NE3PLASMS IN INVERTEBRATES AND COLD-3L30DED VERTEBRATES/,16-1
PATHOBIOLOGICAL MEASJRES C? MARINE POLLUTION EFFECTS/,18-3
•>
USEFULNESS Or CYPRIN3D3N VARIEGATUS AND FUNDULUS GRANDIS IN CARCINOG=NICITY
TESTING: ADVANTAGES AND SPECIAL PROBLEMS/,is-i
COBRTNEYf LEE A.
USEFULNESS 0" CYPRINDDON VAPIEGATUS AND =UMDULUS GRANDIS IN CARCINQGENICITY
TESTING: ADVANTAGES AND SPECIAL PROBLEMS/,13-1
P4GE 65
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AUTHOR INDEX
C
COURTNEYt LEE A., JOINT AUTHOR.
AMERICAN OYSTER (C3A3SQSTREA VlRGINICA) AS AM 'INDICATOR OF CARCINOGENS IN
THE AQUATIC ENVIRONMENT/,11-1
EFFECTS 3F CARCINOGENS, MUTAGENS, AND TERATOGENS OF MONH'JMAN S»=CIES-AQUATIC
ANIMALS CASSTRACT)/,4-l
EFFECTS 3F CARCINOGzMS, MUTSGENS, AND TERATQ3rNS DN NONHUMAN SPECIES
(AQUATIC ANIMALS): <=QURTH ANNUAL RE^Oi^T NCI/EPA COLLARS CRATIVE'PROGRAM/,5-2
LABORATORY EVALUATION >DF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS/,9-1
CROSS, FORD A., JOINT AUTHOR.
BIOMONITDRINS OF COASTAL WATERS—AN OVERVI = W/»10-1
D
DARON, H.H.t JOINT AUTHOR.
GLUCURONSYL- AND SULFQ- TRANSFERASrS IN FISH EXPOSED TO ENVIRONMENTAL
CARCINuGrN/,42-1
INDUCTION OF GLUCURQNIDE AND SUL^ATE TRANSFf l?A SrS BY PHE^308ARBITAL AND
POLYCYCLIC AROMATIC HYC^OCARBONS CABSTRACT}/,42-2
DAVIS, WILLIAM P., JOINT AUTHOR.
EFFECTS 3F C4RCIMOGSNS, MUTAGENS, AND TERATOGENS CF NONHUMAN SPECIES-AQUATIC
ANIMALS (ABSTRACT)/,4-1
EFFECTS OF CARCINOGENS, MUTASENS, AND TERAT03ENS ON NONHUMAN SPECIES
(AQUATIC ANIMALS): FOURTH ANNUAL REPORT NCI/EPA COLLABORATIVE PROGRAM/,5-2
DAVE, CLYDE, JOINT AUTHOR.
MOUSE VS MINMOW: THE FUTURE 0^ PISH IN CARCIH3G-~NICITY TESTING CA FRIENDLY
DISCUSSION/DEBATE 3Y DR. JOHN COUCH AND OR. CLYDE OAWE) - DEBATE: THE FISHY
SIDE/,3-2
OOQSt JESSICA E-, JOINT AUTHOR.
DIFFERENTIAL A3ILITY 05 AMBYSTOM5 TIGRINUM HEPATIC MICROSOMES FROM POLYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES/,1-1
E
ELAM, D,, JOINT AUTHOR.
INDUCTION OF P3LYASOMATIC HYDROCARBON OXIDASE IN MARINE ORGANISMS
CA3STRACT)/,25-l
ELAM, OAVID L.» JOINT AUTHOR.
METABOLITES OF 3ENZOCA)PYRENE IN AROCLOR 125* TREATED MULL5T/.45-1
HIKED FUNCTION OXIDA5E INOUCI3ILITY AND POLYAROMATIC HYDROCARBON METABOLISM
IN THE MJLLST, SEA CATCI3H, AND 5ULF KILLIFISH/,25-2
ELLEMDER, RUDOLPH o., JOINT AUTHOR.
ANALYSIS OF A MARINE FISH CELL LINE FROM A MALE SHEESSHEAO/,14-3
C-BANDINS QF CHR3M3SOM.= S FRCM THREE ESTABLISHED MARlME FISH CELL LIN€S/,1S-1
EXPOSURE OF A TELEOST CELL LINE TO SUSPECTED CiRCINOGENS/,18-2
PAGE 6T
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AUTHOR INDEX
E
RUDOLPH 0,, JOINT AUTHOR.
PRIMARY CELL CULTURES FROM THE TELtQST, CYPRINQDQN V4RIEGATUS: CULTURE
ESTABLISMENT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES/,22-1
••<<*>
ELNEHAEY, ELSAVEO A.
SEPARATION OF TH5 ISDMcRIC PHENOLS Cc 3ENZQ(A)PYRENE BY HIGH-PERFORMANCE
LIQUID CHROMATOGRAPHY/,13-2
V
• fy
SIMPLE HIGH PERF3RMANCE LIQUID CHRQMATOGRAPHY METHOD FOR SEPARATION OF
BENZO(A)?YRENE META3DLITPYRENE PROM MULLET CMUGIL CEPHALUS): PROPERTIES AND
DETECTION! (ABSTRACT)/, 40-1
ENVIRONMENTAL RESEARCH LABORATORY, GULF BREEZE, Ft
INDEX AND ABSTRACTS TO PUBLICATIONS, THE EPA-NCI PROJECT: EFFECTS 0"=
CARCINOGENS, MUTAGcNS, AND T£RAT3GENS ON NON-HUMAN SPECIES (AQUATIC
ANIMALS)/,14-2
>
F
FOSS. STEVEN S.,-JOINT AUTHOR.
AMERICAN OYSTER CCRASS1STREA VIR5INICA) AS AM INDICATOR OF CARCINOGENS'IN :
THE AQUATIC ENVIRONMENT/,11-1
LABORATORY EVALUATION OF MARINE FISHES AS CARCINOGEN ASSAY SUBJECTS/,9-1
G
GOODMAN,.LARRY R-, JOINT AUTHOR.
VERTEBRAL DYSPLASIA IN YOUNG FISH rXPOSED TO THE HERBICIDE T^IFLURALIN/,12-2
GREENWICH, W.*H., JOINT AUTHOR.
BENZIDINE TOXICITY CABSTSACT)/,22-3
GREENWICH, U.U.» JOINT AUTHOR.
EXPOSURE OF TW3 TELEOST SPECIES TO POLYCYCLIC AROMATIC HYDROCARBONS
(ABSTRACT)/,23-2
GREGORY, PAULA E.
ANALYSIS OF A MARINE =ISH CELL LINE FROM A MALE SHEEPSHEAD/,14-3
C-3ANDIN3 3F CHROMOSOMES FROM THREE ESTABLISHED MARINE FISH CELL LINES/,15-1
GRIZZLE, JOHN M. *
CAUSES OF PAPILLDMAS ON FISH EXPOSED TO CHLORINATED SEWAGE EFFLUENT/,15-2
PAPILL3MAS OF BLACK BULLHEADS, ICTALURUS McLftS (RAFINESQUE5, LIVING IN A
CHLORINATED SEWA3E P3ND/,15-3
MIZZLE, JOHN M-, JOINT AUTHOR.
HEPATIC ENZYMES AND TUMOR HISTOPATHOLOGY OF BLACK BULLHEADS WITH
PAPILLQMAS/,44-2
GUESS, M.M.,S JOINT AUTHOR.'
PRIMARY :E'L CULTURES FROM THE T5LEOST, CY?RINODON V4RIEGATUS: CULTURE
£STABLISM5NT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES/,22-1
PAGE 68
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AUTHOR INDEX
H
H»HSEN, OAVIO J., JOINT AUTHOR.
VERTEBRAL DYS^LASIA IN YOUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN/, 12-2
HARSHBARGER, JOHN C.I
HEMATOPQIETIC NEOPLASMS IN INVERTS 3R VTcS AND COLO-BLOODED VERTEBRATES/,16-1
KAUKES» JOYCE U., JOINT AUTHOR.
OBSERVATIONS ON THE ULTR ASTRUCTURE Oc LARGz CELLS ASSOCIATED WITH PUTATIVE
NEOPLASTIC DISORDERS Oc MUSSELS* MYTILUS EDULIS, FROM YA2UINA SAY,
OREGON/,32-2
HEMINGWAY, SUSAN J., JOINT AUTHOR.
BENZOCAJPY.^ENE CONCENTRATIONS IN SlMATIC AND GDNAD TISSUES OF BAY MUSSELS,
MYTILUS =DULIS/,28-2
POLYNUCLEA? AROMATIC HYDROCARBONS IN BIVALVE MOLLUSKS IN BAY" MUSSELS
(MYTILUS EOULI3) F3QM OREGON/,29-2
HENDRICKS, JERRY D.
CHEMICALtCARClN03ENESIS IN FISH/,16-2
HEPATOCARCINOGcNICITY 3F GLANOLESS COTTONSEEDS AMD REFINED C3TTONSSEO OIL TO
RAINBOW TROUT CSALMO GAIRDNERI)/, 17-2
LIVER NEQPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION DXIDASS ENZYMES THE
RAIN30W TROUT FOLLOWING DIETARY EXPOSURE TO 3ENZ3C OPYREME (ABSTRACT)/, 17-1
RAINBOW TROUT CSALMO GAI3DNE3I) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR
EXPERIMENTAL CARCINOGENESI S/, 18-1
HENDRICKS* JERRY D. , JOINT. AUTHOR.
HISTOPATHDLOGY OF =OJR SPONTANEOUS NEOPLASMS IN THREE SPECIES OF SALMONID
FISHES/,26-2
LIMITED EPIZOOTIC OF N EUR03LASTDMA IN CDHO SALMON REaRED IN
CHLORINATEO-3ECHLORIMATED WATER/,26-1
SUMMARY OF TISSUE LE5I3NS IN AQUATIC AMIM^LS INDUCED BY CONTROLLED EXPOSURES
TO ENVIRDNMENTAL CONTAMINANTS, CHEMOTHERAPcUTIC AGENTS, AND POTENTIAL
CARCINOGENS/,26-3
TOBRAMYCIN NSPHRITOXICITY AND LETHALITY IN CDHO SALMON/, 18-2
HILLEBERT, S.A., JOINT AUTHOR.
PRIMARY CELL CULTURES =ROM THE TELEOSTt CYPRINDD3N VARTESATUS: CULTURE
cSTABLISMENT AMD APPLICATION IN CARCINOGEN EXPOSURE STUDIES/ ,22-1
HILLEBERT, -SUSAN A.
EXPOSURE 0? A T2LECST CELL LINE TC SUSPECTED CARCINOGENS/,18-2
DAVID E.
PATHOBIOLOGICAL MEASURES Or MARINE POLLUTION EFFECTS/, 18-3
HOHARD-PEEBLES* PATRICIA N.t JOINT AUTHOR.
ANALYSIS OF A MARINE FISH CELL LINE FROM A M9L5 SH5EPSHE AD/ , 14-3
C-BANDINS OF CHRD^QSOMES FRO" THREE -ESTABLISHED MARINE FISH CELL LINES/,15-1
PAG; 69
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AUTHOR INDEX
j
ELLIOT R., JOINT AUTHOR.
HEMATOPOIETIC NEOPLASMS IN INV ERTE3R ATES AND COLD-3LOODEO VERTEBRATES/,16-1
K
KILGQRE, M., JOINT. AUTHOR.
INDUCTION OF P3LYAROMATIC HYDROCARBON OXIDASE IN MARINE ORGAHISMS
(ABSTRACT)/, Z5-1
KILGQRE, MEL VIM ¥., JOINT AUTHOR.
DETERMINATION OF 1,2-DIOLS BY INDIRECT ATOMIC A3SQRPTION WITH DIGESTED LEAD
PERl3DATE/,45-3
METABOLITES OF BEN 1C CA)PYRSNE IN A^OCLOR 1254 TREATED MULLET/, 45-1
MIXED FUNCTION OXIDASE INDUCIBTLITY AND °QLYARQMATIC HYDROCARBON METABOLISM
IN THE MULLET, SEA CATCISH, AND 3ULF ^KILLI'FISH/, 25-2
KING, KEITH I., JOINT AUTHOR.
3ENZOCA)PYRENE EDDY 3URDEN5 AND THE PREVALENCE OF PR3LIF ERATIVE DISORDERS IN
MUSSELS CMYTILUS EDULIS) IN OREGON/, 31-1
CHEMICAL CARCINCSENS IN THE MARINE ENVIRONMENT. SENZQCAJPYRENE IN
ECONOMICALLY-IMPORTANT BIVALVE MOLLUSKS FROM OREGON ESTUARIES/,33-2
KLINGENSHITH, COURTNEY W., JOINT AUTHOR.
USEFULNESS OF THE SELF-FERTILIZING C YPRINODQNiTID FISH, RIVULUS MARMORATUS AS
AN EXPERIMENTAL SNINUL IN STUDIES INVOLVING CARCINOGEN3SIS , TERAT03ENESIS
AND MUTASENESIS/,19-1
KOENIG, CHRISTOPHER C.
USEFULNESS Of THE SELF-FERTILIZING CYPRINOOONTID FISH, RIVULUS MARMORATUS AS
AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING 3ARCINOGHNESIS, TERATOGENESIS
AND HUTASENESIS/,19-1
L
LARSON, R.E.. JOINT AUTHOR.
T08RAMYCIN NEPHR3TOXICITY AND LETHALITY IN CDHO SALMON/, 38-2
LATOUCHE, DAVID Y,, JOINT AUTHOR.
ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL
CONDITIONS CA8STRACT)/,30-2
LATQUCHE, YVES D.
DETERMINATION DF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EXCHANGE
SESIN NEJTRON ACTIVATION/,20-3
EFFECTS 3F DEP'JRATICN, SIZE AND SEX ON TRACE METAL LEVELS IN SAY
HUSScLS/,23-1
SEASONAL VSRIATI3N IN SOFT TISSUE WEIGHTS AND TRACE METAL BURDENS IN THE BAY
MUSSEL, MYTILUS EDULIS/, 20-4
SEASONAL VARIATIONS OF ARSENIC AMD OTHER TRACE ELEMENTS IN BAY MUSSELS
(MYTILUS EOL'LI5)/,20-2
TRACE METAL LEVELS IN A POPULATION 0? MYTILUS EDULIS FROM YAQUINA BAY,
OREGON. PH.D. THESIS/. 21-1
PAGE 70
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AUHOR ENDfX
L
R«L«
HORPHOL03Y QF PERIPHERAL BLC3D CELLS OF CYPRINQD3N VSRIEGATUS
(ABSTRACT)/,21-2
LO»ELAND, H.N,, JOINT AUTHOR.
HEPATOCARCIN3GENICITY OF GLA<\iOLeSS COTTONSEEDS AND REFINED COTTONSEED OIL TO
RAINBOW TRDUT CSALMC GAIRONERI)/,17-2
LOVELAND, P.M., JOINT AUTHOR.
RAINBOW TROUT CSALNO GAIRDNERI) EMBRYOS? A SENSITIVE ANIMAL MODEL FOR
EXPERIMENTAL CARCIN03ENESIS/,18-1
LOWHAN, FRANK 5., JOINT AUTHOR.
8IDMONITORING OF COASTAL WATFRS--AN OVERVIEW/,10-1
M
HADDOCK, .MICHAEL B., JOINT AUTHOR.
USEFULNESS 0= TH2 SELF-FERTILIZING CYPRINDOCNTID FISH, RIVULUS MARMORATUS AS
AN EXPERIMENTAL ANIMAL IN STUDIES INVOLVING CARCINOGENSSIS, TERATOGENESIS
AND MUTA3ENE5I5/.19-1
MARTIN* B. J.
8ENZIDINE TOXICITY CABSTRACT)/,22-3
MARTIN, BILLT J.
DEVELOPMENT OF A CARCINOGEN ASSAY SYSTEM UTILIZING ESTUARINE FISHES/,22-2
EFFECTS DF PETROLEUM COMPOUNDS ON ESTUARINE "ISHES/,23-1
EXPOSURE 0>= TW3 TELE3ST SPECIES TO P'lLYCYCLIC AROMATIC HYDROCARBONS
(ABSTRACT)/,23-2
PRIMARY CELL CULTURES FRDM THE TSLE03T, CYPRINOD3N VSRIEGATUS: CULTURE
cSTABLIS:4ENT AND APPLICATION IN CARCINOGEN EXPOSURE STUDIES/,22-1
MARTIN, BILLY J., JOINT AUTHOR.
ANALYSIS Q? A MARINE FISH CELL LINE FRO!* A MALE SHEEPSHEAD/f 14-3
C-BANDINb OF CHROMOSOMES FROM TH3EE ESTABLISHED MARINE FISH CELL LINES/,15-1
EM8RYOLOSIC DEVELOPMENT OF THE SHEEPSHEAD MINNOW (CYPRINODON VARIEGATUS3
(ABSTRACT)/,3^-1
EXPOSURE OP A TELEOST CELL LINE TO SUSPECTED CARCINOGENS/,18-2
HISTOLOGY OF THE PCST-PHARYN5EAL DIGESTIVE TRACT OF THE SHEEPSHEAD MINNOW,
CYPRINOOON VARI5SATUS (ABSTRACT)/,35-1
MORPHOLOGY OF PERIPHERAL BLOOD CELLS OF CYPRINODON VARIE3ATUS
(ABSTRACT)/,21-2
SEROLOGIC ALTERATIONS IN CARCINOGEN-EXPOSED TELE3STS: PROCEDURES FOR
PREPARATION AND ANALYSIS OF SAMPLES FROM SMALL FISH/,24-1
NEADOR, C.B.
SERQLOGIC ALTERATIONS IN CARCIN03EN-EXPOSED TELEOSTS: PROCEDURES COR
PREPARATION AND ANALYSIS 0*= SAMPLES FPDM SMALL FISH/,24-1
PAGS 71
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AUTHOR IND'X
M
IELIUS, PAUL
COMPARATIVE BENZ'OC A)P YRENE METABOLITE PATTYS IN FISH AND ROD5NTS/,24-2
INDUCTICNi OF POLYARO?1ATIC HYDROCARBON QXIDASc IN MARINE ORGANISMS
(ABSTRACT)/, 25-1
MIXED FUNCTION OXIDASE INCUCIBILITY AND POLY^KOMATIC HYDROCARBON METABOLISM
IN THE MJLLET, SEA CATFISH, AfO GULF KILLI-I5H/, 25-2
»
HELlUSi PAUL, JOINT AUTHOR.
8ENZDCA3PYRENS METABOLISM IN HEPATIC S-9 FRACTIONS OF 4ROCLO* 1254-TREATED
MULLET CMUGIL CEPHAL JS)/»43-3
CAUSES OF PAPILLOMAS DN FISH EXPOSED TO CHLORINATED SEWAGE EFFLUENT/, 15-2
DETERMINATION UF 1,2-OIOLS BY INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAD
PERIODATE/,45-3
HEPATIC ENZYMES AND TU*OR HISTOPATHOLOGY OF 3LACK BULLHEADS w'lTH
PAPILLOMAS/,44-2
INDIRECT ATOMIC ABSORPTION S3ECT ROMETRIC ASSAY FOR EPOXIDE HYDRQLASE/,44-3
METABOLITES OF BEN2GC A)P YR2N5 IN AROCLOR 1254 TREATED MULLET/,45-1
RESPONSES OF THE HEPATIC ENZYMES 0= A TELEOST FISH TO TRANS-STILBENE OXIDE
TREATMENT/,45-2
SIMPLE GAS CHROM4TOG3APHIC MSTHCO FO?? THE STUDY QF ORGANIC SOLVENTS:
MOISTURE ANALYSIS, HYG^G SCOPICIT Y, AND EVAPORATION/,44-1
MEYERS* THEODORE ; R.
HISTQPATHOLOGY 0= FOUR SPONTANEOUS NEOPLASMS IN THREE SPECIES OF SALMONIO
FISHES/,26-2
LIMITED EPIZOOTIC OF NEURO 3LASTOMA IN COHO SALMON REARED IN
CHLQRINA IE D-DE CHLORINATED WATER/,26-1
SUMMARY OF TISSUE LESIONS IN AQUATIC ANIMALS INDUCED 3Y CONTROLLED EXPOSURES
TO ENVIRONMENTAL CONTAMINANTS, CHEMOTHERA?EUTIC AGENTS, AND POTENTIAL
CARCINOGENS/,26-3
HEYERSt THEODORE R., JOINT ^AUTHOR.
LIVER NEOPLASIA AND INDUCTION 0s HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE
RAIN30W TRDUT FOLLOWING DIETARY EXPOSURE TO BENZ 0
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AUTHOR INDEX
M
MICHAEL C.
BENZ3(A)*YSEME CONCENTRATIONS IN MUSSELS (MYTILUS EDULIS) FROM YAQUINA BAYt
OREGON DURING JUNE 1976 - HAY 1978/V31-2
3ENZO(A)?YREN£ CONCENTRATIONS IN SOMATIC ANC GONAD TISSUES OF BAY MUSSELS,
MtTILUS EDULIS/, 28-2
CELLULAR PROLIFERATIVE DISORDER IN OYSTERS OSTRfA CHIL-NSIS) FROM CHILOE,
CHILEt SOUTH A1E3ICA/ ,30-1
CHEMICAL CARCINOGENS IN BIVALVE MOLLUSKS FROM OREGON ESTUARIES/,32-1
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT, B£NZDCA>PYRENE IN
ECONOMICALLY-IMPORTANT BIVALVE M3LLUSKS FROM OREGON ESTUARIES/,33- 2
CONCENTRATIONS C= UNSU3STITUTED POLYCYCLIC AROMATIC HYDROCAR3QNS IN
SOFTSHELL CLAMS -ROM COOS BAY, OREGON, USA/,27-1
i
CONCENTRATIONS 0= UNSUBSTITUTED POLYNUCLEAR AROMATIC HYDROCARBONS IN BAY
MUSSELS CMYTILUS EDULIS) FRCM OREGON, USA/,27-2
ENVIRONMENTAL CONTAMINANTS A"^D THE OCCURRENCE OF CERTAIN PATHOLOGICAL
CONDITIOMS (ABSTRACT)/, 30-2
HAEMIC NEOPLASMS OF 3AY MUSSELS^ MYTILUS EDULISt F»0^ OREGON: OCCURRENCE,
PREVALENCE, SE ASDNALITY, AND HISTOPATHQLQGIC AL PRCGR SSSIQN/, 27-3
OBSERVATIONS ON THE ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTATIVE:
NEOPLASTIC DISORDERS Op MUSSELS, MYTILUS EDULIS, FROM YAQUINA BAY,
OREGON/, 32-2
POLYMUCLEAR AROMATIC HYDROCARBONS AND CELLULAR PROLIFERATIVE DISORDERS IN
BIVALVE MOLLUSCS FROM OREGON ZST'JARISS/t 29-1
POLYNUCLEAR AROMATIC HYDROCARBONS IN BIVALVE MOLLUSKS IN 3AY MUSSELS
CMYTILUS EDULIS) FROM OREGCN/,29-2
PRELIMINARY STUDIES TO EVALUATE THE POTENTIAL OF USING EMBRYO AND LARVAL
STAGES OF THE GOOSE 3ASNACLE, POLLICIPES PDLYMERUS FOR MARINE
aiOASSAY3/,33-l
MIX, MICHAEL C.t> JOINT AUTHOR.
DETERMINATION OF VANADIUM IN A MARINE MOLLUSC USING A CHELATING ION EXCHANGE
RESIN NEUTRON ACTIVATION/, 20-3
EFFECTS OF DEPUPATIOM, SIZE AND SEX ON TRACE METAL LEVELS IN BAY
MUSSELS/,20-1
EFFECTS OF NAPHTHALENE ON GLUCOSE METABOLISM IN THE EUROPEAN FLAT OYSTER,
OSTREA EOULIS/,35-2
EFFECT* 3F SODIUM CHLORIDE CONCENTRATION ON ELECTROPHORETIC PATTERNS OF
ADDUCTOR MUSCLE PROTEINS FROM 3IVALVE MOLLUSCS/,41-1
ION-EXCHANGE TECHNIQUE FOR CONCENTRATING AMMONIA FROM SMALL VOLUMES OF
SEAWATER/,35-3
PAGE 73
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AUTHOR INDEX
M
MX* MICHAEL C., JOINT AUTHOR,
PYROLYTIC DEPOSITION CF POLYNUCLEAR AROMATIC HYDROCARBONS DU = TO SLASH
BURNING IN CLEAR-CUT SITES/,43-1
REGENERATION OF * ADI AT IQN-DAMA SED DIGESTIVE TISSUES IN JUVENILE PACIFIC
OYSTERS (CRASSQSTREA GIGAS)/,46-l
SEASONAL VARIATION IN SOFT TISSUE WEIGHTS AND TRACE METAL BURDENS IN THE SAY
MUSSEL, MYTILUS EDULIS/,20-4
SEASONAL VARIATIONS OF ARSENIC AND OTHER TRACE ELEMENTS IN BAY'MUSSELS
(MYTILUS EDULIS)/,20-2
SIMPLE AND INEXPENSIVE METHOD =OR MEASURING TNTEoRATzD LIGHT ENERGY/,43-2
THIN-LAYER SEPARATION! DF CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, AND
THE AMINO ACID TAURINE/,37-1
UPTAKE AND ACCJMJLATICN 3F NAPHTHALENE BY THE OYSTER OSTREA EDULIS, IN A
FLOW THROUGH SYSTEM/,36-i
MORENO, MARK
EMBRYOLOGIC DEVELOPMENT OF THF SHEEPSHEAD MINNOW (CYPRINODON VARIEGATUS5
(ABSTRACT)/,34-1
N
NIXON, J.E., JOINT AUTHOR.
HEPATOCA3CINDG5MCITY OF GLANDLESS COTTONSEEDS AMD REFINED COTTONSEED OIL TO
RAINBOW TROUT (SALMO G4IRDNERI)/,17-2
RAINBOW TROUT CSALMO GAIRDNERI) EM3RYQS: A SENSITIVE ANIMAL'MQOEL FOR
EXPERIMENTAL CARC INOGENESIS/,18-1
P
PATTQN, JOHN S.
CAN TISSUE ANOMALIES THAT OCCUR IN MARINE FISH IMPLICATE SPECIFIC POLLUTANT
CHEMICALS?/,34-2
PAHLOHSKI, N.E.,*JOIMT AUTHOR.
HEPATOCARCINOG=NICITY OF GLANDLESS C3TTONSEE3S AND REFINED COTTONSEED OIL TO
RAINBOW TROUT
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AUTHOR INDEX
R
RILEY, RONALD T.
STIMULATORY EFFtCT OF NAPHTHALENE ON GLUCOSE TRANSPORT IN THE OYSTER
(ABSTRACT)/,37-2
THIN-LAYER SEPARATION OF CITRIC ACID CYCLE INTERMEDIATES, LACTIC ACID, AND
THE AMINO ACID TAURI^E/,37-1
UPTAKE AMD ACCUMULATION JF NAPHTHALENE SY THE OYSTER OSTREA EDULIS, IN A
FLOW THROUGH S YSTE »V, 36-i
RILEY, RONALD T,, JOINT AUTHOR.
CHEMICAL CARCINOGENS IN THE MA3IME ENVIRONMENT, BENZGCA)PYRENE IN
ECONOMICALLY-IMPORTANT BIVALVE M3LLUSK5 FROM OREGON ESTUARIES/,33-2
ROSE, FRANCIS L., JOINT AUTHOR.
DIFFERENTIAL ABILITY 0= AMBYSTQMA TIGRINUM HEPATIC MICROSOMES CROM PJ3LYCYLIC
AROMATIC HYDROCARBONS AND AROMATIC AMINES/,1-1
S
SARADAMBAL, D.V., JOINT AUTHOR.
SLUCURONSYL- AND SULFD- TRANSFEREES IN FISH EXPOSED TO ENVIRONMENTAL
CARCINOGEN/,42-1
SCANLAN.JR.A., JOINT AUTHOR.
RAINBOW TROUT CSALMO GAIRDNERI) EM3RYOS: ft SENSITIVE ANIMAL MODEL F3R
EXPERIMENTAL CA»CIN03ENESIS/,18-1
SCHAEFFER, RANDY L., JOINT AUTHOR.
BENZOCA)PYRENE,CONCENTRATIONS IN MUSSELS (MYTILUS EDULIS) ?ROM YAQUINA BAY,
OREGON CJRING JUNE 1976 - MAY 197B/»31-2
BENZDCA)?YRENE CONCENTRATIONS IN SOMATIC AND GQN5D TISSUES Oc BAY MUSSELS,
MYTILUS EDULIS/,28-2
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT, 8£NZOCA)PYR£NE IN
ECONjMICALLY-IMPJRTAMT 3IVALVE MQLLUSKS PROM OREGON ESTUARIES/,33-2
CONC5NTRATICNS OP UNSU3STITUTED POLYCYCLIC AROMATIC HYDROCARBONS IN
SOFTSHELL CLAMS =R3M CC05-BAY, OREGON, USA/,27-1
CONCENTRiTIC^S 0? UNSU3STITUTEO PQLYNUCLEA5 AROMATIC HYDROCARBONS IN BAY
MUSSELS CMYTILUS EDULIS) FROM OREGON, USa/,27-2
ENVIRONMENTAL CONTAMINANTS AND THE OCCURRENCE OF CERTAIN PATHOLOGICAL
CONDITIONS (ABSTRACT)/,30-2
PQLYNUCLEAR AR3MATIC HYDROCARBONS IN BIVALVE MQLLUSKS IN BAY MUSSELS
(MYTILUS EDULIS) FROM OREGON/,29-2
UPTAKE AND ACCUMULATION OF NAPHTHALENE BY THE OYSTER OSTREA EDULIS, IN A
FLOW THROUGH SYSTEM/,36-1
SCHNEIDER, S.R.
T03RAMYCIN NEPHR3TOXICITY AND LETHALITY IN COHO SALMON/,38-2
CORRELATION1" MIXED-FUNCTION JXIDASE ACTIVITY WIT^ ULTRASTR'JCTURAL CHANGES
IN THE LIVER Or A MARINE FISH/,40-2
PAG€ 75
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AUTHOR INDEX
S
SCROORt «- PETER
EFFECTS DF TRICHLOROPROPENE OXIOE ON THE METABOLISM OF BENZOC /OPYRENE BY
S-HETHYL:HOLANTHRENE- AND PHENOBARBITAL-TREATED MULLET CMUGIL CEPHAtusJt A
MARINE FISH (A3STRACT)/, 39-1
EXPOSURE 0<= FISHES T 3 3E.MZOYRENE BY HIGH-PERFORMANCE
LIQUID C,HROMATGGRAPHY/,13-2
SIMPLE HIGH PERFORMANCE LIQUIO CHROPATOGRAPHY METHOD FDR SEPARATION OF
BENZO(A)PYREME METABOLITES ( ABSTRACT)/ , 14-1
PAG: 75
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AUTHOR INDEX
S
SCHHEDLER, I.E., JOINT AUTHOR, N , TUTM_ TM .
PAPILLOMAS OF BLACK BULLHEADS, ICTALURUS MELAS (RAFIMESQUE) f LIVING IN A
CHLORINATED SEWAGE PDND/,15-3
SCOTT, A.L., JOINT AUTHOR.
PAPILLOMAS OF BLACK BULLHEADS, ICTALURUS MELAS C RAFIMESQUE), LIVING IN A
CHLORINATED SEWASE POND/,15-3
SHELTON,, DENNIS M, , JOINT AUTHOR.
LIVER NEOPLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE
RAIN30W TROUT FOLLOWING DIETARY EXPOSURE TO BSNZOCA)PYR£NE C ABSTRACT)/, 17-1
SINNHUBER, R.O., JOINT AUTHOR.
HEPATCCARCINOGENICITY OF GLANDL'ESS COTTONSEEDS AND REFINED COTTONSEED OIL TO
RAIN30W TRCUT CSftLMO GAIRCN5RI)/ , 17-2
RAINBOW TR3UT CSALMO GAIRDNERI) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR
EXPERIMENTAL CiR-INOGENESIS/ , 13-1
SINNHUBER, RUSSELL 0., JOINT AUTHOR.
LIVER NE3PLASIA AND INDUCTION OF HEPATIC MIXED FUNCTION OXIDASE ENZYMES THE
RAINSQd TROUT FOLLOWING DIETARY EXPOSURE TO 3ENZO< A)PYR£N£ {ABSTRACT)/ , 17-1
SMITH, A.C.
EFFECTS OF SODIUM CHLORIDE CONCENTRATION ON ELECTROPHQRETIC PATTERNS OF
ADDUCTOR MUSCL" 3RQTEINS FROM BIVALVE MOLLUSCS/,41-1
SMITH, CHARLIE E.v JOINT AUTHOR.
HEMATOPGIETIC NEOPLASMS IN INVERTEBRATES AND COLD-3LOODED VERTEBRATES/, 16-1
SPARKS, ALBERT K. , JOINT AUTHOR.
OBSERVATIONS OM THE ULTRASTRUCTURE OF LARGE CELLS ASSOCIATED WITH PUTATIVE
NEOPLASTIC DISORDERS 0- MUSSELS, MYTILUS EOULlSt FROM YAQUINA SAY,
OREGON/, 32-2
SRIVASTAVA, M.
EFFECT OF 2, 3-OX ID5-3, 3 , 3-TRICHLOROPRQPANE ON BAP METABOLISM IN MULLET
(ABSTRACT)/, 41-2
SRIVASTAVA, MEERA, JOINT AUTHOR.
EFFECTS OF TRICHLQSQ?RQP£NE OXIDE ON THE METABOLISM OF BENZQC A)PYRENE 3Y
3-METHYLCHOLANTH.RENS- AND PHENOBARBITAL-TREATED MULLET
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AUTHO* INDEX
S
$«LLIVAN» TIMOTHY J.
SIMPLE AND INEXPENSIVE METHOD FOR MEASURING INTEGRATED LIGHT ENERGY/,43-2
T
TAN, BARRIE
B£NZOCA3?YRE*E M-TABUISM IN HEPATIC S-9 FRACTIONS OF AR3CLCR 1254-TREATED
MULLET C^UGIL CE 3HALUS)/,4 3-3
• e
DETERMINATION DF 1,2-DIOLS 3Y INDIRECT ATOMIC ABSORPTION WITH DIGESTED LEAD
PERIQDATE/,45-3
HEPATIC ENZYMES AND TUMOR HISTOPATHOLOGY OF 3LACK BULLHEADS WITH
PAPILLOMAS/,44-2 ,
INDIRECT ATOMIC 15S03PTICN SPECTROMETRTC ASSAY FOR EPQXIDE HYDROLASE/,44-3
METABOLITES OF 33NZOCA)PYR £NE IN ARCCLOR 1254 TREATED MULLET/,45-1
RESPONSES OF THE HEP4TIC ENZYMES 0~ 4 TELEOST FISH TC TRANS-STIL3ENE OXIOE
TREATMENT/,45-2
SIMPLE GAS CHROMATOGRAPHIC METHOD FOR THE STUDY 3F ORGANIC SOLVENTS:
MOISTURE ANALYSIS, HYGR OSCOPICIT Y, AND EVAPORATION/, 44-1
TAN, BARRIE* JOINT AUTHOR.
MIXED FUNCTION OXIOASE INDyCIBILITY ANO POLYAROMATIC HYDROCARBON METABOLISM
IN THE MJLLET, SEA CATFISH, AND GULF KILLICISH/, 25-2
TRENHOLM, STEVEN R.
EFFECTS OF X- AND GAMMA IRRADIATION 3N THE JUVENILE PACIFIC 3YSTER,
CRASSOSTREA GI3AS- '^.S. THESIS/,47-1
REGENERATION 0= RADIATION-DAMAGED DIGESTIVE TISSUES IN JUVENILE PACIFIC
OYSTcRS CCRA5S3STREA GIGAS)/,46-l
TRENHOLM, STEVEN R., JOINT AUTHOR.
8ENZQ(A)?YRENE BODY BURDENS AND THE PREVALENCE OF PRCLIFERATI VE DISORDERS IN
MUSSELS CMYTILUS EDULIS) IN OREGON/,31-1
CHEMICAL CARCINOGENS IN THE MARINE ENVIRONMENT. 5ENZOCA )PYRENE IN
ECONOMICALLY-IMPORTANT BIVALVE MOLLUSKS FROM OREGON ESTUARIES/, 33-2
W
MALES, J.H.,1 JOINT AUTHOR.
RAINBOW TROUT CSAL^.C GAIRDMERI) EMBRYOS: A SENSITIVE ANIMAL MODEL FOR
cXPERIMEMTAL CARCINOGENcSIS/. 1 3-1
WANG, SHOO-LIH, JOINT AUTHOR.
GLUCURONSYL- A'JD SUL=0- TR ANSP£RAS = S IN BISH EXP3SEO T3 ENVIRONMENTAL
CARCINOGEN/, 42-1
WILSON* J.F. , JOINT AUTHOR.
INDUCTION OF CLUCURONIDE AND SULFATE TRANS-ERASES BY PHENQBARBITAL AND
POLYCYCLIC AROMATIC HrCUDCARBONS CA3STRaCT>/, 42-2
AS AN INDICATOR OF CARCINOGENS IN
THE AQUATIC ENVI^ONMcMT/, 11-1
PAGE 73
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AUT'HOS INDEX
w
yINSTEAD, JAMES T.t JOINT AUTHOR.
CONCURRENT NEDPIASTIC *ND PRQTTSTAN DISORDERS IN THE AMERICAN OYSTER
(CRASSQSTREA VIRJINICA)/f11-2
VERTtBRAL OYSPLASI4 IN YUUNG FISH EXPOSED TO THE HERBICIDE TRIFLURALIN/t 12-2
Z
ZIEGLER, PAUL* JOINT AUTHOR.
SIMPLE GAS CHROMaT^G^AOHIC METHOD =0^? THC STUDY OF ORGANIC S^LVEMTS:
MOISTURE ANALYSIS, HYG30SCQPICITY, SND EVAPORATION/,44-1
PAGE 79
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