United States
              Environmental Protection
              Agency
              Environmental Monitoring
              and Support Laboratory
              PO Box 15027
              Las Vegas NV 89114
EPA-600 4-79 061
September 1979
              Research and Development
xvEPA
Pesticides and
Polychlorinated Biphenyls
in the Atchafalaya Basin,
Louisiana

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                   RESEARCH  REPORTING SERIES

 Research reports of the Office of Research and Development, U.S. Environmental
 Protection Agency, have been grouped into nine series.  These nine broad categories
 were established to facilitate further development and application of environmental
 technology.   Elimination of traditional grouping was consciously planned to foster
 technology transfer and a maximum interface in related fields.  The nine series are:


       1.   Environmental Health Effects Research
       2.   Environmental Protection Technology
       3.   Ecological Research
       4.   Environmental Monitoring
       5.   Socioeconomic Environmental Studies
       6.   Scientific and Technical Assessment Reports (STAR)
       7.   Interagency Energy-Environment Research and Development
       8.   "Special" Reports
       9.   Miscellaneous Reports
 This report has been assigned to the ENVIRONMENTAL MONITORING series.This series
 describes research conducted to develop new or improved methods and instrumentation
 for the identification and quantification of environmental  pollutants at the lowest
 conceivably significant concentrations.  It also includes studies to determine the ambient
 concentrations of pollutants in the environment and/or the variance of pollutants as a
 function of time or meteorological factors.
This document is available to the public through the National Technical Information
Service, Springfield, Virginia  22161

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                                              EPA-600/4-79-061
                                              September 1979
   PESTICIDES AND POLYCHLORINATED BIPHENYLS
      IN THE ATCHAFALAYA BASIN, LOUISIANA

                      by

       Stephen C. Hern, Victor W. Lambou
        Monitoring Operations Division
Environmental Monitoring and Support Laboratory
           Las Vegas, Nevada  89114

                      and

                   Han Tai
        Pesticide Monitoring Laboratory
       Bay St. Louis, Mississippi  39520
ENVIRONMENTAL MONITORING AND SUPPORT LABORATORY
      OFFICE OF RESEARCH AND DEVELOPMENT
     U.S. ENVIRONMENTAL PROTECTION AGENCY
           LAS VEGAS, NEVADA  89114

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                                 DISCLAIMER


     This report has been reviewed by the Environmental  Monitoring and Support
Laboratory-Las Vegas, U.S. Environmental Protection Agency, and approved for
publication.  Mention of trade names or commercial products does not constitute
endorsement or recommendation for use.

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                                  FOREWORD
     Protection of the environment requires effective regulatory actions that
are based on sound technical and scientific information.   This information
must include the quantitative description and linking of pollutant sources,
transport mechanisms, interactions, and resulting effects on man and his
environment.  Because of the complexities involved, assessment of specific
pollutants in the environment requires a total systems approach that tran-
scends the media of air, water, and land.  The Environmental Monitoring and
Support Laboratory-Las Vegas contributes to the formation and enhancement of
a sound integrated monitoring data base through multidisciplinary, multimedia
programs designed to:

          •  develop and optimize systems and strategies  for monitoring
             pollutants and their impact on the environment

          •  demonstrate new monitoring systems and technologies by
             applying them to fulfill  special monitoring  needs of the
             Agency's operating programs

     This report presents the distribution and concentration of pesticides
and polychlorinated biphenyls in the Atchafalaya Basin.   These data can be
utilized to formulate plans to minimize the environmental impact of proposed
hydrological modifications.  This report was written for  use by Federal,
State, and local government agencies concerned with water quality analysis,
monitoring, and/or regulation.  Private industry and individuals similarly
involved with the Atchafalaya Basin will find the document useful.  For
further information contact the Water and Land Quality Branch, Monitoring
Operations Division.
Geojxje
                                  je B.  Morgan
                                  Director
               Environmental  Monitoring  and Support Laboratory
                             Las Vegas,  Nevada
                                   iii

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                                  CONTENTS


Foreword	iii
Figures	   vi
Tables	vii
Acknowledgments  	 viii
Introduction 	    1
Conclusions  	    2
Recommendations  	    3
Description of Basin 	    4
Materials and Methods  	   14
   Station Coding  	   14
   Station Selection and Sample Collection 	   14
   Water and Bottom Sediment Analysis  	   15
   Fish Analysis	   19
Results and Discussion 	   23
   General Findings	   23
   DDT	   25
   Polychlorinated Biphenyls 	   34
   "Chlordane	   39
   Aldrin-Dieldrin .	   41
   Distribution Patterns 	   42
References	   46
Appendix A	   48

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                                   FIGURES
Number                                                                   Page
   1     Base map of the Atchafalaya Basin	    6
 2-7     Station locations in the Atchafalaya Basin  	    7
   8     Discharge hydrograph of the Atchafalaya River at
         Simmesport, Louisiana, 1963-1971   	   13
   9     The location of stations where DDT,  including its
         derivatives (DDE, TDE), was detected  	   30
  10     The location of stations where PCB 1254 was detected   	   36
  11     Location of stations where chlordane was detected  	   40
  12     Location of the stations where dieldrin-aldrin was
         detected	43

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                                    TABLES
Number                                                                   Page
                                                     -«
   1      Number of water and bottom sediment samples collected
         at each sampling station ....................  16

   2     Organochlorine compounds with their minimum detection
         levels without Florisil  cleanup  ................  20

   3     Organophosphorus compounds with their minimum  detection
         levels without Florisil  cleanup  ................  21

   4     Pesticide and PCB content of fish collected from  Henderson
         Lake and the Courtableau area  .................  24

   5     Station, date, and sample type where DDT including  its
         metabolites (DDE, TDE)  was detected in water and  bottom
         sediment samples ........................  26

   6     The distribution of DDT in bottom sediment  samples  by
         geographical  area  .......................  29

   7     Geographical  area where DDT and its metabolites  (DDE,
         TDE) were detected in fish samples ...............  32
   8     Station,  date,  and  sample  type  where  PCB  1254 was detected
         in water  and bottom sediment  samples  ..............  35

   9     The distribution  of PCB  1254  in water and bottom sediment
         samples by geographical  area  ..................  37

  10     Geographical  area where  PCB 1254 was  detected in fish
         specimens  ...........................  38

  11     Station,  date,  and  sample  type  where  chlordane was detected
         in water  and bottom sediment  samples  ..............  41
  12     Geographical  area  where  dieldrin-aldrin was detected  in
         fish specimens  .........................  44
                                    VI1

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                               ACKNOWLEDGMENTS


     The authors wish to extend their appreciation tc C.  D.  Kennedy,
W. 6. Mitchell, G. J. Sand, and G. F. Gardner, who were charged with the
arduous task of analyzing the fish, water, and sediment samples.  Their
dedication and competency provided the data that is the backbone of this
report.

     Without the cooperation of Dan Tabberer (U.S. Department of the Interior,
Fish and Wildlife Service), who supervised the collection of the fish specimens
and authorized the release of the unpublished data, the valuable information
pertaining to fish would not be included in this report.
                                   vi i i

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                                INTRODUCTION
     The Atchafalaya Basin in south-central Louisiana is a large alluvial
basin that has national significance as a multiple resource.  It derives this
significance principally from possessing high-quality habitats for fish and
wildlife, being a semiwilderness area of high recreational value, and
functioning as a floodway for the lower Mississippi River.  To meet the need
for flood control, the Army Corps of Engineers has proposed hydrological mod-
ifications (channel training or channelization) for the Basin.  The Basin's
present hydrological cycle and complex water circulation patterns support
one of the world's most highly natural productive areas.

     In response to a request by the Governor of Louisiana and a joint U.S.
Congressional resolution, the U.S. Environmental Protection Agency, U.S. Army
Corps of Engineers, and U.S. Department of the Interior are conducting a wa-
ter and land quality study in the Atchafalaya River Basin.  The study's objec-
tives are to assess the potential impact of the proposed hydrological modifi-
cations and to develop alternative land and water management plans to accomo-
date floodflows and maintain an acceptable level of environmental quality
for the Atchafalaya Basin.

     The collection and analysis of samples for pesticides and polychlorinated
biphenyls (PCB's) were included in the Atchafalaya River Basin Water and Land
Study.  From 1974 to 1977, 743 samples were collected from 118 stations in the
Atchafalaya Basin.  Water, bottom sediment, and fish samples were analyzed for
9 organophosphorus compounds and 18 organochlorine compounds.  The purpose of
this report is to present the distribution and importance of pesticides and
polychlorinated biphenyls in the Atchafalaya Basin.

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                                 CONCLUSIONS


1.  The Atchafalaya Basin is relatively free of organophosphorus  pesticides.
    No organophosphorus compounds were detected in any fish,  water,  or  sedi-
    ment samples.

2.  Only a few organochlorine compounds—aldrin, dieldrin,  polychlorinated
    biphenyls (PCB's), chlordane, and DDT and its derivatives—are  present  in
    the bottom sediments of the Atchafalaya Basin.  With the  exception  of
    PCB's, none of the compounds was detected in water samples.   Aldrin,
    dieldrin, PCB's, and DDT and its derivatives were found in fish.

3.  The pattern of pesticide distribution that emerges in the Atchafalaya
    Basin reflects the agricultural  activity within or affecting  the  various
    hydrological units.  The majority of the samples in which pesticides were
    identified were collected from the Courtableau, West Atchafalaya  Floodway,
    Fausse Pointe Basin, Morganza Floodway, and the Fordoche  areas,  which
    contain agriculture or are directly impacted by agricultural  drainage.

4.  The highest concentration of PCB's was found in bottom  sediments  from
    waterways of an industrial area southeast of Morgan City, Louisiana.

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                               RECOMMENDATIONS
     Reductions in water level  fluctuations of the Atchafalaya Basin result
in increased agricultural  activities within the Basin.   The pattern of pesti-
cide distribution that, emerges  in the Atchafalaya Basin reflects agricultural
activity within the Basin  or drainage from agricultural areas  outside the
Basin.  Therefore, it is recommended that the natural  regime of water level
fluctuations that characterize  the Atchafalaya Basin Floodway  be maintained
and that increased drainage of  agricultural lands into the Basin be discour-
aged to avoid increased levels  of pesticide contamination.

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                            DESCRIPTION OF BASIN


     The Atchafalaya Basin is a large, shallow depression located within the
deltaic plain of the Mississippi River in southern Louisiana (Figures 1-7).
It comprises a 4,662-kilometerZ (km2) lowland area confined between natural
levee ridges that delineate the present and former courses of the Mississippi
River.  The overall dimensions of the study area are approximately 72 km by
193 km with elevation ranging from about 15 meters (m) to sea level.  Maximum
channel depths of approximately 49 m below mean sea level occur in scour pools,
but the trunk channel of the Atchafalaya River is more typically 12 to 24 m
below ground level with natural depths in lakes and bays being about 0.6 to
2.5 m.

     Water enters the Basin from two major sources.  Approximately 30 percent
of the Mississippi River water flows through the Old River control structure,
which eventually joins with the Red River to form the main stem of the
Atchafalaya River.  The Atchafalaya River discharge shows both seasonal and
annual variation.  The average monthly discharge hydrography for the Atchafalaya
River at Simmesport for the period 1963-71 indicates the seasonality of flows
(Figure 8).  The flow generally begins to increase in December or January and
crests in the period between April and early June.  The flows normally drop
off sharply to their seasonal lows in late summer and early fall.  The cyclic
nature of flow also describes the typical annual regime of flooding for the
entire Basin.

     In the upper one-third of the Basin, the channel-levee complex consists
of a single, well-defined trunk channel bounded by levee ridges.  Stage fluc-
tuations are great, but overbank flooding is infrequent in this area.  Basi-
cally, flooding of this area occurs at high-water periods when water "stacks
up" and then flows north between the outside east and west protection levees
and the inner levees associated with the main channel.

     In the middle Basin, the trunk channel and levees lose their identity as
they enter a very large depression where the flow and sediments of the system
are no longer confined.  This results in a decrease in velocity and an increase
in cross-sectional area where velocity losses occur.  The stream deposits sed-
imentary particles forming bars and shoals, forcing the river into a branching
pattern.  This portion of the Basin is an intricate mosaic of shallow-water
lakes of various sizes and shapes, bayous, sloughs, channels, and distribu-
taries.  In the lower portion of the Basin, the river channel regains its
identity.  The total river flow, combined with rainfall from within the Basin,
passes through two outlets, the lower Atchafalaya River outlet at Morgan City
and the Wax Lake outlet.

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     Extensive swarrps and freshwater-to-saline marshes characterize the
Atchafalaya complex below Morgan City.  The marshes and swamps have an intri-
cate tidal-drainage'network dependent on local runoff and tidal conditions.

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                           Atchafalaya  Basin, LA
Fifjurc 1.  Case nap of the Atchafalaya Fasin.
                        6

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  -
 re
 ro
 • n
 , t
 Qj
  i

  3
  )
 n
 OJ
 i >
  -
 11
 ro

   •
 01
'   :
 ,  •;
 QJ
 1/1

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1

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           MONTHLY  AVERAGE  1963-1971 (since
            completion of Old River  structure)
   400 i—
   300
i  200
vt
O 16O5
   100
Annual
  Average
          i
                              i
                                     High Water
                                     Low  Water
                                                       12
                                       4.799
                                            Q

                                            I
M
        MJJ

          MONTH
                                                N
Figure 8.  Discharge hydrograph of the Atchafalaya  River at
          Simmesport, Louisiana, 1963-71 (Gagliano and
          van Beek, 1975).
                       13

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                            MATERIALS AND METHODS
STATION CODING
     A unique, four-place alphanumeric STORET code (e.g., E032)  was assigned
to each sampling station.  STORET (STOrage and RETrieval) is the U.S.  EPA
computer system that processes and maintains water quality data.  The  first
letter of the station code identifies a geographic area of the Basin as
follows:

     A - North of U.S. Highway 1 and Simmesport, Louisiana.

     B - The main stem of the Atchafalaya River between Simmesport and
         Krotz Springs, Louisiana.

     C - West Atchafalaya Floodway system, north of U.S. Highway 190,
         south of State Highway 1, and between the west protection levee
         and main stem levee.

     D - Morganza Floodway system, north of U.S. Highway 190 and between
         main stem levee and east protection levee.

     E - The Atchafalaya Floodway, bounded by the east and west protection
         levees and by U.S. Highway 190 on the north and U.S.  Highway  90
         on the south.

     F - Outside the west protection levee and north of U.S. Highway 90.

     G - All areas east of the east protection levee, north of
         U.S. Highway 90, and south of U.S. Highway 190.

     H - All areas of the Basin south of U.S. Highway 90.

     The next three digits designate the primary station number within a
given geographic area.  In Figures 2 through 7, abbreviated station numbers
were utilized because of space requirements (e.g., A-l instead of A001).


STATION SELECTION AND SAMPLE COLLECTION

     Stations were selected to define water quality in the Atchafalaya Basin,
including the distribution of pesticides, and to examine the productivity and
energy export of habitats and the contribution of these to the Basin and estu-
arine areas (Figures 1-7).  These stations provide representative data on dis-


                                    14

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tribution of pesticides and PCB's, because they are:

     1.  Geographically distributed throughout the Basin,

     2.  Located at the major inlet and outlets to the Basin,

     3.  Located at major water exchange points within the Basin,

     4.  Located in the Verret Basin adjacent to the east guide levee,

     5.  Located in the drainage basin to the west of the Atchafalaya
         River, and

     6.  Located in the freshwater swamps south of Morgan City.

     Water samples for analysis were collected at the surface while bottom
sediment samples were collected by using a grab-type bottom sampler.   Samples
were placed in 1-quart (0.95-liter) Mason jars equipped with Teflon lids.
Before sample collection, the Mason jars and Teflon lids were washed with  soap
and water and rinsed first with distilled water and then twice with reagent-
grade hexane.

     Fish specimens were collected using an electrofishing device.   Specimens
were collected in the Fordoche area from Henderson Lake and in the  Courtableau
area.

     A total of 721 samples (451 water and 270 bottom sediment) was collected
from 116 stations for analysis (Table 1), while 7 fish specimens were analyzed
from the Fordoche area and 15 from the Courtableau area.


WATER AND BOTTOM SEDIMENT SAMPLE ANALYSIS


Water Extraction

     Five hundred milliliters (ml) of the water sample were extracted by
shaking for 1 minute in a separatory funnel with three consecutive  100-ml
portions of methylene chloride.  The methylene chloride extracts were fil-
tered into a 500-ml Erlenmeyer flask through a filter tube containing anhy-
drous sodium sulfate and a prewashed glass-wool plug.  The sodium sulfate
was then rinsed with 50 ml of methylene chloride.  A Snyder column  was added,
and the apparatus was placed on an explosion-proof hotplate.  The extract  was
taken to near dryness, 50 ml of hexane were added, and the extract  was taken
to near dryness again.  This process was repeated three times, and  the extract
was concentrated to a final volume of approximately 5 ml.   This extract was
poured into a graduated centrifuge tube, and the volume was adjusted  to 10 ml.

     If cleanup was necessary, a portion of the 10 ml extract was cleaned  up
through Florisil® for"electron-capture gas chromatographic detection  of organo-
Florisil® - Trademark of   Floridin Company.

                                    15

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TABLE 1.  NUMBER OF WATER AND BOTTOM SEDIMENT SAMPLES COLLECTED AT EACH
                           SAMPLING STATION
Station
A001
A002
A003
A004
B001
C001
C002
C003
C004
C005
C007
COOS
D001
E001
E002
E003
E004
E005
E006
E007
E008
E009
E010
E011
EOT 2
EOT 3
E014
EOT 5
EOT 6

Water
4
5
1
4
5
5
3
3
7
5
4
4
4
7
6
4
4
3
4
1
6
3
3
8
1
4
1
3
3

Sediment Total
0
0
0
4
0
3
2
1
6
4
2
5
2
6
5
4
4
2
3
1
4
3
3
5
0
4
0
2
2

4
5
1
8
5
8
5
4
13
9
6
9
6
13
11
8
8
5
7
2
10
6
6
13
1
8
1
5
5
(Continued)
Station
E017
E018
E019
E020
E021
E022
E023
E024
E025
E026
E027
E028
E029
E030
E031
E032
E033
E034
E035
E036
E037
E038
E039
E040
E041
E042
E043
E044
E045

Water
3
1
5
5
2
0
2
2
3
2
5
4
1
2
3
6
3
3
4
3
1
2
2
3
4
3
6
4
3

Sediment Total
2
1
5
3
2
1
1
2
3
2
4
1
0
2
3
4
3
3
2
2
0
0
0
1
2
1
3
2
1

5
2
10
8
4
1
3
4
6
4
9
5
1
4
6
10
6
6
6
5
1
2
2
4
6
4
9
6
4
(Continued)
                                 16

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TABLE 1.  Continued
Station
E046
E047
E048
E049
E050
E051
E052
E053
E054
E055
E056
E057
E058
E059
E060
E061
E062
E063
E064
E065
E072
FOOT
F002
F003
F004
F005
F006
F007
F008
F009
Water
3
6
4
3
5
6
4
4
4
2
4
4
' 4
5
1
2
3
4
1
2
5
5
2
5
5
6
6
3
4
4
Sediment
2
2
0
2
3
4
1
2
1
1
2
2
3
2
0
0
0
2
0
0
6
4
1
3
1
4
3
2
3
3
Total
5
8
4
5
8
10
5
6
5
3
6
6
7
7
1
2
3
6
1
2
11
9
3
8
6
10
9
5
7
7
Station
F010
F011
6001
G002
G003
G004
G005
6006
6007
G008
6009
G010
6011
6012
6013
6014
H001
H002
H003
H004
H005
H006
H007
H008
H009
H010
H011
H012
HOI 3
HOI 4
Water
0
6
5
5
6
3
5
5
4
6
7
3
3
4
2
6
2
2
6
5
7
6
6
4
5
3
4
4
4
1
Sediment
1
6
3
3
4
2
3
4
3
3
4
2
2
2
1
4
0
1
3
3
4
4
4
1
3
2
2
2
2
0
Total
1
12
8
8
10
5
8
9
7
9
11
5
5
6
3
10
2
3
9
8
11
10
10
5
8
5
6
6
6
1

       17

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chlorine compounds.  The remaining extract was reserved for injection on a
flame photometric detector for organophosphorus compounds.  Flame photometric
detection normally did not require a cleanup.


Bottom Sediment Extraction

     A 100-g subsample was taken from a thoroughly mixed sample.  The sub-
sample was extracted with 200 ml of 3:1 hexane:isopropanol solvent by shaking
4 hours on a reciprocating shaker.  The isopropanol was removed by three dis-
tilled water washes, and the hexane extract was dried through anhydrous sodium
sulfate.  The sample extract was then stored at low temperature for subsequent
gas-liquid chromatographic analysis.

     If Florisil cleanup was necessary, a separate aliquot of the extract
without Florisil cleanup was reserved for analysis on the flame photometric
detector for detection of organophosphorus pesticides.  The remaining extract
was cleaned up on a Florisil column for electron capture detection of organo-
chlorine compounds.


Removal of Sulfur Interference from Bottom Sediment Samples

     Sulfur removal from bottom sediment samples was according to the method
of Goerlitz and Law (1971).


Florisil Cleanup

     An appropriate aliquot of the extract was fractionated through a 15-g
Florisil column into two fractions by use of 100 ml of 10 percent methylene
chloride in hexane and 100 ml of methylene chloride for fractions one and
two, respectively.

     Methylene chloride was removed by concentration of each extract to low
volume under a three-ball Snyder column, addition of 100 ml hexane, and con-
centration again to low volume.  After two additions of hexane, the methylene
chloride was essentially removed.  Each extract volume was adjusted to 2.5 ml
for separate injection on the gas-liquid chromatograph.


Gas-Liquid Chromatography

     Analyses were performed on gas chromatographs equipped with tritium foil
electron affinity detectors for organochlorine compounds and thermionic or
flame photometric detectors for organophosphorus compounds.  A multiple-column
system employing polar and nonpolar columns was utilized to identify and con-
firm pesticides.  The following equipment and specifications were used to
analyze the samples:

     Gas Chromatographs:  Hewlett-Packard 402A
                                      18

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     Columns:
     Carrier Gases:
     Temperatures:
Hewlett-Packard 402B
"Tracer" MT-220

Glass, 6 mm outside diameter by 4 mm inside diameter,
183 cm long, packed with one of the following:
5 percent OV-210 on 80/100 mesh chromasorb W, HP;
3 percent DC-200 on 100/200 mesh Gas-Chrom Q; or
1.5 percent OV-17/1.95 percent QF-1 on 100/200
mesh sepelcoport

5 percent methane-argon at a flow rate of approxi-
mately 80 ml/min; prepurified nitrogen at a flow
rate of approximately 80 ml/min

Thermionic detector housing    250°C
Detector electron capture and
   flame photometric
                          Injection port
                          Column OV-210
                          Column DC-200
                          Mixed column
    200°C

    250°C

    166°C

170-175°C

185-190°C
Recoveries

     Recovery values on water and bottom sediment ran from 80 to 100 percent
depending on the compound and substrate involved.

     Specific compounds for which analysis was made are listed in Tables  2
and 3 with their minimum detection levels.  The minimum detection levels  were
decreased approximately tenfold when Florisil  cleanup was utilized.


FISH ANALYSIS

Extraction and Partition Procedures

     The whole fish specimen was blended into  a creamy homogeneous mixture  and
50 g were transferred into a 500-ml Erlenmeyer flask.  After  the addition of
200 ml of acetonitrile, the sample was agitated on an automatic  shaker  at
medium speed for 4 hours.  The sample was transferred into a  250-ml  separ-
atory funnel by filtering it through glass wool  with 50 ml  of hexane added
to the sample.  The sample was agitated for 3  minutes, and after the sample
settled, the lower layer (acetonitrile) was drained into a 500-ml  separatory
funnel.  One hundred ml of acetonitrile-saturated hexane was  added,  agitated,
the sample settled, and then the lower layer drained.  This process  was re-
peated three times, draining all the lower layers into the same  separatory
                                      19

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      TABLE  2.  OR6ANOCHLORINE COMPOUNDS WITH THEIR MINIMUM DETECTION
                       LEVELS WITHOUT  FLORISIL CLEANUP
Compound
Aldrin
Benzenehexachl ori de
(hexachlorocyclohexane, BHC)
Benzenehexachl ori de - technical
grade (hexachlorocyclohexane, BHC)
Captan
Chlordane technical
Dacthal
Dieldrin
Endosulfan
Endrin
Gamma - chlordane
Heptachlor
Heptachlor epoxide
Hexachlorobenzene (HCB)
0, P1 - DDE
0, P1 - DDT
PCB's (reported as PCB 1254)
P, P1 - DDE
P, P1 - DDT
P, P1 - TDE
Toxaphene
Treflan
Minimum Detection
Water
(yg/ liter)
0.1

0.1

0.5
0.2
0.5
0.2
0.1
0.2
0.1
0.1
0.1
0.1
0.1
0.2
0.4
0.5
0.2
0.4
0.3
0.5
0.1
Levels
Sediment
(yg/kg)
10

10

50
20
50
25
10
20
10
10
10
10
10
20
40
50
10
10
10
50
10
1
 Minimum detection levels were decreased approximately tenfold when  Florisil
 cleanup was utilized.
                                    20

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      TABLE 3.  ORGANOPHOSPHORUS COMPOUNDS WITH THEIR MINIMUM DETECTION
                       LEVELS WITHOUT FLORISIL CLEANUP
                                                Minimum Detection Levels
                                           Water                     Sediment
Compound                                (yg/liter)                    (ng/kg)


Abate                                       0.1                          10

DEF                                         0.1                          10

Diazinon                                    0.1                          10

Ethion                                      0.5                          50

Ethyl parathion                             0.5                          50

Malathion                                   0.25                        25

Methyl parathion                            0.1                          10

Phorate                                     0.1                          10

Trithion                                    0.5                          50
 Minimum detection levels were decreased approximately tenfold when Florisil
 cleanup was utilized.
funnel (300 ml total).  Acetonitrile-saturated hexane (25 ml)  was  added  and
the sample was agitated.  Then the acetonitrile layer (lower)  was  drained into
a 500-ml Erlenmeyer flask.  The acetonitrile was concentrated  to about 10 ml
on an explosion-proof hotplate.  One hundred ml of hexane were added to  the
sample, and it was concentrated to 10 ml  on an explosion-proof hotplate.  The
hexane addition and concentration processes were repeated.  A  Snyder column
was rinsed with a small amount of hexane, with the hexane rinse draining into
the flask containing the sample. . The contents of the flask were transferred
into a 15-ml centrifuge tube with a Teflon-lined screwcap and  diluted to 15 ml
with hexane.


Florisil Cleanup

     A Shell column (joint 24/40, 26 cm long) was prepared with 20 g of
Florisil, topped with 1.25 cm of sodium sulfate and vibrated to settle the
packing material.  Prior to utilization of a given lot of Florisil, the  lot
was verified with a mixture of pesticide standards to assure that  separation
was completed and the recoveries were quantitive.


                                    21

-------
     The Florisil column was washed with a 100 ml of hexane.  A 250-ml
Erlenmeyer flask equipped with a 24/40 joint was placed under the column,
and the contents of the 15-ml centrifuge tube were transferred to the Florisil
column.  The centrifuge tube was rinsed and its contents added to the column
once the sample had passed the sodium sulfate layer.  After all the extract
had passed through the sodium sulfate layer, it was eluted with 150 ml of
equal proportions of hexane and methylenedichloride.  After the elution of
the column had been completed, boiling beads were added to the collection
flask and Snyder column.  The sample was evaporated to 50 ml and then an
additional 50 ml of hexane were added to the sample.  The sample was evapor-
ated to 5 or 10 ml and transferred to a test tube.


Gas-Liquid Chromatography

     The fish samples were analyzed for chlorinated compounds on a Hewlett-
Packard 5710A/7671A system utilizing the following specifications:

                 Oven temperature - 175°C
                 Detector temperature - 300°C
                 Injection port - 250°C
                 Column used - 3 percent OV-210

                 Attenuation - 16 x 102
                 Gas flow - 25 cm /min of methane and argon
                 Detection limits - 10 parts per billion (ppb) (wet weight)

     The samples were analyzed for organophosphorus compounds utilizing a
flame photometer incorporated with a Tracer MT-220 using the following spec-
ifications:

                 Oven temperature - 180°C

                 Detector temperature - 190°C

                 Injection port - 275°C

                 Column used - 3 percent DC 200

                 Attenuation - 8 x 102
                 Gas flow - 25, 80, and 55 cm /min of oxygen, hydrogen, and
                     nitrogen, respectively

                 Detection limits - 10 ppb (wet weight)


Recoveries

     The specific compounds for which analysis was made in the fish specimens
were identical to the compounds for which analysis was made in the water and
bottom sediment samples (Tables 2 and 3).  However, the minimum detection limit
ranged from 10 yg/kg to 50 yg/kg (wet weight) for all compounds.

                                    22

-------
                           RESULTS AND DISCUSSION
GENERAL FINDINGS

     Appendix A summarizes the results of all  water and bottom samples  that
were collected in the Atchafalaya Basin and analyzed for pesticide and  PCB
content.  The appendix is organized by site-date combinations  and  is  subdi-
vided into the major categories of water and bottom sediment samples.   Under
the major subheadings, the classes of compounds—organochlorine and organo-
phosphorus—are listed.  Preceding the classes of compounds  is the treatment
method employed for the water or bottom sediment sample.   Treatment method  A
refers to analysis without initial Florisil cleanup, while treatment  method B
refers to analysis subsequent to Florisil  cleanup.   When a specific compound
was detected, it was listed under the appropriate class of compounds, and its
concentration was recorded.

     Organophosphorus pesticides are relatively short-lived  in the environ-
ment, and none (abate, DEF, diazinon, ethion,  ethyl  parathion, malathion,
methyl parathion, phorate, trithion) was detected in any of  the water or
bottom sediment samples.  It should be noted that intermittent sampling for
short-lived pesticides would have a very low likelihood of detecting  periodic
pesticide inputs.

     Organochlorine compounds—benzenehexachloride  (BHC),  captan,  dacthal,
endosulfan, endrin, heptachlor, heptachlor epoxide,  hexachlorobenzene (HCB),
toxaphene, and treflan—were not detected  in any of the water  or bottom sedi-
ment samples, even though they are relatively long-lived in  the environment.
DDT, including its metabolites (DDE, TDE), was found in 78 bottom  sediment
samples and was the most common compound detected.   PCB's  were found  in 16
water samples and 6 bottom sediment samples.  It was the next  most common
compound detected.  Other compounds detected include chlordane (found in
seven bottom sediment samples) and dieldrin (found  in one  bottom sediment
sample).

     Fifteen fish specimens were collected from the  Courtableau area  north  of
U.S. Highway 190, and seven specimens were collected from  Henderson Lake in
the Fordoche area.  Species collected were bluegill  (Lepomis macrockirus},
blue catfish (Ictalurus furcatus), bowfin  (Amia oalva), largemouth bass
(Mioropterus salmoides), river carpsucker  (Carp-Lodes carpio],  redear  sunfish
(Lepomis miarolophus), spotted gar (Lepisosteus productus),  and white crappie
(Pomoxis annulapi-s}.  Table 4 lists the pesticide or PCB content in yg/kg of
wet weight by species and collection area.
                                   23

-------
         TABLE 4.  PESTICIDE AND PCB CONTENT OF FISH COLLECTED FROM
                   HENDERSON LAKE AND THE COURTABLEAU AREA
Compound
(yg/kg wet weight)
Location
G
and T
•o
Species <
Henderson Lake:
Bluegill
Bowfin
Largemouth bass 10
Largemouth bass -
Redear sunfish
Spotted gar
Spotted gar
Courtableau Area:
Blue catfish
Bluegill
Largemouth bass -
Largemouth bass -
River carpsucker -
River carpsucker -
River carpsucker -
River carpsucker -
River carpsucker -
Spotted gar
Spotted gar
White crappie
White crappie
White crappie
White crappie** -
Chlordane
Dieldrin
i

2*
11
-
2*
5*
4*
8*
-
3*
-
-
6*
4*
10
8*
-
8*
4*
12
7*
-
&-
O LU
r- «* Q
.c m o
O CM
3-^-0.
O. CO

-------
      Compounds that were detected in fish specimens were PCB's, aldrin,
dieldrin, chlordane, and DDT and its metabolites.  These compounds were
also detected in water or bottom sediment samples.

      The organochlorine compounds benzenehexachloride (BHC), captan, dacthal,
endosulfan, endrin, heptachlor epoxide, hexachlorobenzene (HCB), toxaphene,
heptachlor, and treflan were not found in fish.

      Also, no organophosphorus compounds—abate, DEF, diazinon, ethion, ethyl
parathion, malathion, methyl parathion, phorate, and trithi on—were found in
the fish.

      The absence of endrin from fish, water, and bottom sediment samples is
notable since it was the agent responsible for the catastrophic fish kills
in the lower Mississippi River Basin in 1963-64 (U.S.  Department of the
Interior, 1969).  In the 1963-64 comprehensive study,  the endrin concentration
ranged from 0.0 to 70.0 yg/kg in fish blood and from 0.02 to 0.36 yg/liter
in water.

      Other compounds that were detected in the fish specimens,included PCB's,
aldrin, dieldrin, chlordane, and DDT and its metabolites (p, p  DDE, p, p  TDE,
o, p  DDE).  These pesticides and the PCB's were the only compounds detected in
the water or the sediment samples.  Their distribution and importance will be
discussed in subsequent sections.


DDT

      In this discussion DDT [1,1, l-trichloro-2, 2-bis(p-chlorophenyl)
ethane] refers to the sum total of DDT and its metabolites TDE and DDE.
Because DDT is a highly persistent chemical, bioconcentrates in  aquatic or-
ganisms used for human consumption, and is considered  a potential  human car-
cinogen, levels of DDT in water should be kept as low  as feasible.  The water
quality criterion is 0.001 ppb for the protection and  propagation of fish and
other aquatic life (EPA, 1976).

      Aquatic organisms generally exhibit acute toxicity to  DDT  at levels in
the 1-ppb range.  Some 96-hour LCSQ'S (the dose of a toxicant that is fatal

to 50 percent of the organisms under test conditions)  that have  been reported
are:  crayfish, Orconectes naisf 0.24 ppb (Sanders, 1972), and largemouth
bass, Micropterus salmoideej 2.0 ppb (Macek and McAllister,  1970).

      DDT will definitely bioconcentrate.  Reinert's (1970)  study of fish in
Lake Michigan demonstrated concentration factors of up to 2  million times
above background levels.  Other fish bioconcentration  factors demonstrated
under laboratory conditions are in the thousandfold category instead of the
millionfold range (Hansen and Wilson, 1970).

      In this study DDT.was detected in 78 of 270 possible bottom sediment
samples and was found at 38 of 98 locations.  DDT was  never  detected in 451
water samples collected from 118 stations (Table 5).  Figure 9 details the


                                     25

-------
     TABLE  5.   STATION,  DATE
  METABOLITES  (DDE,  TDE), WAS
,  AND SAMPLE TYPE WHERE  DDT,  INCLUDING  ITS
 DETECTED IN WATER AND BOTTOM SEDIMENT  SAMPLES
Station
A004
C001


C002

C003
C004




C005
C007

COOS




D001

E001




E002

Date
12-18-75
6-10-75
11-18-75
12-17-75
11-18-75
12-17-75
11-18-75
12-09-74
4-17-75
6-11-75
9-10-76
9-30-76
6-11-75
11 -29-75
4-19-77
9-01-76
9-30-76
11-29-76
4-19-77
5-24-77
4-14-75
6-11-75
5-28-74
11-15-74
12-09-74
4-14-75
12-15-75
5-28-74
11 -15-74

Water
(yg/liter)
__
ND
ND
--
ND
—
ND
ND
—
ND
ND
ND
ND
ND
ND
—
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
—
ND
ND
Sample Type
Bottom Sediment
(uq/kg)
21
50
90
115
70
32
50
50
20
20
10
70
10
20
20
50
150
140
40
100
20
10
130
20
240
100
217
140
210
(continued)
ND = DDT and its metabolites not detected in sample.
                                    26

-------
TABLE 5.  (Continued)
Station
E002


E003



E004


E005

E006

E009
E011
E013
E020
E022
E025
E030
E031
E033
E044
E049
E050
E051
FOOT


Date
6-11-75
12-15-75
3-08-77
11-19-74
12-09-74
6-11-75
12-15-75
11-19-74
6-11-75
12-15-75
11-19-74
6-12-75
11-19-74
6-12-75
11-19-74
6-12-75
11-19-74
11-15-74
12-12-75
11-20-74
6-13-75
11-20-74
12-12-75
12-16-75
4-18-75
4-18-75
4-15-75
11-19-74
12-09-74
12-15-75

Water
(yg/ liter)
ND
—
—
ND
ND
—
ND
ND
ND
—
ND
ND
ND
ND
ND
ND
ND
ND
—
ND
ND
ND
—
ND
ND
ND
ND
ND
ND
ND
Sampje Type
Bottom Sediment
(vg/kg)
100
73
30
100
110
30
54
140
140
79
120
30
70
20
70
6
40
20
16
60
12
40
7
4
10
10
10
180
30
232
                                       (continued)




        27

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                            TABLE 5.   (Continued)
Station
F003

F005


F008
F009
F010
F011





G005
G011
GO! 3
G014
H007
Date
11-19-74
12-12-75
6-13-75
11-15-75
12-12-75
6-14-75
12-12-75
12-17-75
9-01-76
9-30-76
11-29-76
3-08-77
4-19-77
5-24-77
6-14-75
4-18-75
4-18-75
4-18-75
4-15-75

Water
(yg/ liter)
NO
ND
ND
ND
ND
ND
ND
—
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Sample Type
Bottom Sediment
(yg/kg)
80
179
20
40
20
20
6
76
40
90
140
20
40
40
20
10
10
10
10
locations where DDT was detected in fish and bottom sediment samples collected
in the Atchafalaya Basin (to accurately depict the geographical  distribution
of DDT in the Basin only one symbol per station was used in the  figure even
if multiple occurrences of DDT were detected at a given station).   The figure
also illustrates eight different geographical areas, which are shaded and
labeled with letter symbols:  West Atchafalaya Floodway north of U.S. High-'
way 190 (WAF); Morganza Floodway north of U.S. Highway 190 (MF); Courtableau
(CT); Fordoche (FD); Atchafalaya Basin Floodway south of U.S. Highway 190 and
exclusive of Fordoche (ABF); Fausse Pointe Basin (FPB); Verret Basin (VB);
and the area south of Morgan City (SMC).

     Table 6 lists the range and mean concentration of DDT in bottom sediments
for each geographical area shown in Figure 9.  The mean and range calculations

                                    28

-------
                TABLE  6.  THE  DISTRIBUTION  OF  DDT  IN  BOTTOM SEDIMENT SAMPLES BY GEOGRAPHICAL AREA
ro
10
Geographical Area
Symbol ,
WAF
MF
CT
FD
ABF
FPB
VB
SMC
Description
West Atchafalaya Floodway
Morganza Floodway
Courtableau
Fordoche
Atchafalaya Basin Floodway
Fausse Points Basin
Verret Basin
South of Morgan City
Concentration
ug/kg
Range
2.5 - 96.0
15.0 - 15.0
16.6 -110.5
0.0 -112.8
0.0 - 13.3
0.0 - 86.3
0.0 - 6.6
0.0 - 2.5
Mean
47.5
15.0
67.7
44.2
2.0
14.1
1.7
0.3
Number of Sampling
Stations Where
Detected/
Total Number of
Sampling Stations
7/7
1/1
3/3
8/12
11/43
4/8
4/14
1/9
Percent of
Sampling
Stations
Where Detected
100
100
100
66
25
50
27
11

-------
                                   Atchafalaya Basin, LA.
                                             Baton Rouge
                                               5   0   5  10
Figure 9.  Location of stations where DDT  (including DDE, TDE) was detected.
                                   30

-------
were derived by using station means from the appropriate geographical area.
The ratio of the number of sampling stations where DDT was detected to the
total number of sampling stations is presented along with the percentage rep-
resented by the ratio.  Fish specimens were collected from Henderson Lake in
the Fordoche area and the Courtableau area.  Table 7 lists the DDT (including
its metabolites) content in ug/kg of wet weight by species and denotes the
area of collection.

     The Courtableau area (CT), represented by three sampling stations,  had
the highest mean DDT bottom sediment concentration (67.7 ug/kg).   This area
receives water from an extensive agricultural  area north of U.S.  Highway 190.
This area is dewatered by Bayou Courtableau, which drains into the Fordoche
area, and also, to a minor extent, by water flowing south into the Fausse
Pointe Basin.  DDT was detected in all 15 fish specimens collected from  the
Courtableau area.  The mean DDT concentration in the specimens was 367 ug/kg,
which is not surprising when one considers the bioconcentration capacities of
fish and the relatively high DDT concentrations in the sediments  of this area.

     The West Atchafalaya Floodway (WAF), represented by seven stations, had
the second highest DDT bottom sediment mean of 47.5 ug/kg, and DDT was de-
tected at all sampling stations in the area.  The West Atchafalaya Floodway
is contained by levees on three sides and by high land on the fourth side.
The area receives only local  precipitation except during periods  of major
flooding along the lower Mississippi River.  The area supports agricultural
activities that are assumed to be the source of the contamination.

     The Morganza Floodway (MF) is the eastern counterpart of the West
Atchafalaya Floodway.  It is also a self-contained area that has  agricul-
tural activity and receives only local precipitation.  Only one sampling
station was located in this area, and bottom sediments from this  station
had a mean DDT concentration of 150
     Eight sampling stations were located in the Fausse Pointe Basin (FPB),
which is west of the Atchafalaya Basin and receives water from the Courtableau
area and from local drainage.  Agricultural  activity is present in this  area.
The mean DDT concentration in bottom sediments was 14.8 ug/kg  with a range  of
0.0 to 86.3 ug/kg.

     The Verret Basin (VB) ,  represented by 14 stations, is east of the
Atchafalaya Basin Floodway.   Local  precipitation is the only source of water
to the area.  The Verret Basin has  agricultural  activity within it, but  such
activity is much more limited than  in the Fausse Point  Basin.   This is reflected
in the lower mean of 1.7 ug/kg DDT  in bottom sediments.

     Local runoff and the Atchafalaya River water contribute to the annual
flooding of the Fordoche area (FD).   The flooding of the area  is a modified
backwater flooding regime.  Atchafalaya River water,  during high-water months,
enters across the southern boundary  through the West Atchafalaya Basin pro-
tection levee barrow pit after diversion from the river into Bayou Butte
La Rose (Bayou La Rose)  (Figure 4).   The water then flows north through
Henderson Lake and northern  swamps  in accordance with the river stage. In-
                                   31

-------
   TABLE  7.   GEOGRAPHICAL  AREA  WHERE  DDT AND  ITS METABOLITES  (DDE, TDE)
                       WERE DETECTED IN  FISH SAMPLES
Geographical
Area
Henderson
Henderson
Henderson
Henderson
Henderson
Henderson
Henderson
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Species of
Fish
Bluegill
Bowfin
Largemouth bass
Largemouth bass
Redear sunfish
Spotted gar
Spotted gar
Blue catfish
Bluegill
Largemouth bass
Largemouth bass
River carpsucker
River carpsucker
River carpsucker
River carpsucker
River carpsucker
Spotted gar
Spotted gar
White crappie
White crappie
White crappie
White crappie**
DDT and Its
Metabolites
(vg/kg wet weight)
19
3*
117
25
13
83
44
484
250
284
210
128
289
331
1517
162
486
630
163
222
99
250
 *  Unconfirmed result (below detection limit).
**  Homogenous mixture of four white crappie.
                                    32

-------
troduction of local runoff from outside the Fordoche area occurs across the
northern boundary (West Atchafalaya Floodway) and through the Bayou Courta-
bleau structure (Figure 9).  The Courtableau area contributes the greatest
percentage of water to the area.  Dewatering of the Fordoche area occurs by
flow through the West Atchafalaya Basin protection levee barrow pit at Butte
La Rose and is also dependent on river stages.

     Twelve stations were chosen to represent the Fordoche area (FD).   The
Fordoche area had a mean concentration of 44.2 yg/kg DDT in bottom sediments
with a range of 0.0 to 110.6 yg/kg.  The influence of water entering the area
from the Courtableau area is best represented by sampling station F001  where
a mean of 110.5 yg/kg DDT was found in bottom sediments, while water entering
from the WAF would be represented best by sampling stations COOS and C007
where mean values of 50 and 20 yg/kg DDT were found in bottom sediments,
respectively.  Therefore, considering both the relative contribution of water
to the Fordoche area and the higher mean concentrations of DDT in bottom sed-
iments from the Courtableau stations, it would appear that the majority of
DDT present in the Fordoche area originates from the Courtableau area.   DDT
was detected in all seven fish specimens collected from Henderson Lake  in the
Fordoche area.  The mean concentration of DDT in these specimens was 43.4 yg/kg.

     The Atchafalaya Basin Floodway (ABF) south of U.S. Highway 190 and exclu-
sive of the Fordoche area had a mean concentration of only 2.0 yg/kg DDT in
bottom sediment with a range of 0.0 to 13.3 yg/kg.  DDT was detected in bottom
sediments at only 11 of 43 stations.

     Gagliano and van Beek (1975), in August 1973, sampled water and bottom
samples from six sampling stations for pesticides in the lower Atchafalaya
Basin Floodway.  DDT was the only pesticide detected in their investigation.
The mean concentration was 30.2 yg/kg DDT in bottom sediment while the  mean
level in the water was 1.9 yg/liter.

     The area south of Morgan City (SMC) had the lowest mean concentration
(0.3 yg/kg) of DDT in bottom sediments, with a range of 0.0 to 2.5 yg/kg.
DDT was found at only one of nine stations.  Water enters this area basically
from the Atchafalaya River and, to a limited extent, from Verret Basin.

     Table 6 graphically illustrates the distribution and dispersion of DDT
in the bottom sediments of the Atchafalaya drainage system.   Bottom sediments
from the Courtableau area and the West Atchafalaya Floodway had relatively
high mean DDT levels of 67.7 and 47.5 yg/kg, respectively.   Waters from these
two areas drain into the Fordoche area and contribute to the high mean  bottom
sediment content of 44.2 yg/kg.  However, the Atchafalaya Basin Floodway,
exclusive of the Fordoche area, which is basically fed by the Mississippi
River system, had a low mean bottom sediment content of only 2 yg/kq.   The
area below Morgan City had the lowest mean concentration (0.3 yg/kg) of DDT
in bottom sediments of all the geographical areas sampled.

     Three other relatively discrete hydrological systems—the Morganza
Floodway, Fausse-Pointe Basin, and Verret Basin—were sampled and their mean
bottom sediment DDT values of 15.0, 14.1, and 1.7 yg/kg, respectively,  appear
to reflect the agricultural activity within each system.

                                    33

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POLYCHLORINATED BIPHENYLS


     Polychlorinated biphenyls (PCB's) are a class of compounds produced by
the chlorination of biphenyls and are registered in the United States under
the trade name Aroclor®.  Their chemical properties are dependent on their
degree of chlorination, which can be determined by their numerical nomencla-
ture, e.g., Aroclor 1242, Aroclor 1254.  The first two digits represent the
molecular type, while the last two digits represent the average percentage by
weight of chlorine.  PCB's are relatively nonflammable, have low heat exchange
and dielectric properties, and are basically used in capacitors and transfor-
mers in industries.  The polychlorinated biphenyl water quality criterion is
0.001 yg/liter for the protection and propagation of freshwater and marine
aquatic life (EPA, 1976).

     The acute effects of PCB's have been determined on a number of aquatic
organisms.  Studies indicate that freshwater invertebrates are generally more
susceptible to acute toxic effects of PCB's than fish (Stalling and Mayer,
1972; Nebeker and Puglisi, 1974).  Ninety-six-hour LC5Q values of 15 yg/liter

for Aroclor 1242 and 7.7 yg/liter for Aroclor 1254 were determined for newly
hatched fathead minnows, Pimephales promelas (Nebeker et al., 1974).  In
another study 15-day LCg_ values were determined for the bluegill, Lepomie

maeroahimsj using Aroclor 1242, 1248, and 1254 with the respective results
54, 76, and 204 yg/liter (Stalling and Mayer, 1972).  Nebeker and Puglisi
(1974) established a 3-week LCcn for the invertebrate Daphn-ia magna of
1.3 yg/liter.                 50

     In this study, all  polychlorinated  biphenyls are reported as PCB 1254.
PCB 1254 was detected in water or bottom sediment samples at 19 locations
(Table 8).  Sixteen out of 360 water samples collected at 112 stations and
6 out of 196 bottom sediment samples collected at 96 stations were positive.

     Figure 10 shows the location where PCB 1254 was detected in fish, water,
and sediment samples (to accurately depict the geographical distribution of
PCB 1254, only one symbol per location was used even if multiple occurrences
of PCB were detected at that location).  The figure also illustrates nine
different geographical areas, which are shaded and labeled with letter symbols:
West Atchafalaya Floodway north of U.S. Highway 190 (WAF); Morganza Floodway
north of U.S. Highway 190 (MF); Courtableau (CT); Fordoche (FD); Atchafalaya
Basin Floodway south of U.S. Highway 190 and exclusive of Fordoche (ABF);
Fausse Pointe Basin (FPB); Verret Basin  (VB); the industrial area south of
Morgan City (IA); and south of Morgan City and exclusive of the industrial
area (SMC).

     Table 9 lists the range and mean concentration of PCB 1254 in bottom
sediments and water samples for each geographical area shown in Figure 10.
The mean and range calculations were derived by using station means from the
appropriate geographical area.  The ratio of the number of sampling stations
where PCB 1254 was detected to the total number of sampling stations is pre-
sented along with the percentage represented by the ratio.  Table 10 lists


                                   34

-------
TABLE 8.  STATION, DATE, AND SAMPLE  TYPE WHERE PCB  1254 WAS
          DETECTED IN WATER AND BOTTOM  SEDIMENT SAMPLES
Station
C004
E002
E004
E008
E010
EOT 3
E025
E044
E047
E049
E051
F004
F009
G006
G008
H003
H003
H004
H005

H006
Date
9-30-76
6-11-75
6-11-75
4-17-75
4-17-75
6-11-75
6-13-75
4-18-75
4-16-75
1-20-75
6-14-75
6-13-75
6-14-75
12-11-74
12-11-74
5-15-75
12-11-75
12-11-75
3-19-75
6-14-75
6-14-75
Sample Type
Water
(yg/liter)
0.1
1.3
2.8
0.1
1.2
0.6
0.6
0.1
0.1
0.1
0.7
1.8
5.4
0.7
0.4
ND
ND
ND
1.6
ND
ND

Sediment
(yg/kg)
ND
ND
ND
ND
ND
ND
ND
—
—
--
ND
ND
ND
ND
30
140
220
41
—
68
53
                           35

-------
                             Atchafalaya Basin, LA.
                      Krotz
                     Springs
                                        Baton Rouge

                                         5   O   5  10
       °/f of Mexico
                                                Bayou
                                       Bayou     Black
                                       Cherte
Atchafalaya
   River
      • Sediment
      DWater
      *Fish
Figure 10.   The location of stations where PCB 1254 was detected.

                              36

-------
          TABLE 9.  THE DISTRIBUTION OF PCB 1254 IN WATER AND BOTTOM SEDIMENT SAMPLES BY GEOGRAPHICAL AREA
CO
•-J
Water Samples
Concentration
(yg/liter)
Area
WAF
MF
CT
FD
ABF
VB
FPB
IA
SMC
Range
0.00-0.02
0.00-0.00
0.00-0.00
0.00-0.43
0.00-0.14
0.00-0.18
0.00-5.40
0.00-1.60
0.00-0.00
Mean
0.002
0.000
0.000
0.120
0.014
0.020
0.900
0.070
0.000
Sediment Samples
Stations That Percent of
Showed PCB/ Stations Concentration
Number of Showing Cyg/Hter)
Stations PCB
1/7
0/1
0/2
3/13
6/53
2/14
2/8
1/4
0/6
14
0
0
23
11
14
25
25
0
Range
0.0-0.0
0.0-0.0
0.0-0.0
0.0-0.0
0.0-0.0
0.0-10.0
0.0-0.0
20.5-180.0
0.0-0.0
Mean
0.0
0.0
0.0
0.0
0.0
0.7
0.0
62.4
0.0
Stations That Percent of
Showed PCB/ Stations
Number of Showing
Stations PCB
0/6
0/1
0/3
0/12
0/42
1/14
0/8
4/1
0/5
0
0
0
0
0
7
0
100
0

-------
concentration of PCB 1254 in yg/kg of wet weight by species and indicates
the area of collection.

     PCB 1254 was not detected in water or sediment samples in the Courtableau
area (CT).  However, PCB 1254 was detected in one out of seven fish samples
from this area (Table 10).  PCB 1254 was detected only once in water at seven
stations in the West Atchafalaya Floodway and was not detected in bottom
sediments.  PCB 1254 was not detected in any water or bottom sediment samples
from the one sampling station in the Morganza Floodway (MF).
              TABLE 10.  GEOGRAPHICAL AREA WHERE PCB 1254 WAS
                         DETECTED IN FISH SPECIMENS
                                                               Concentration
Location                          Species                   (vg/kg wet weight)


Henderson Lake                 Redear sunfish                      55

Courtableau                    River carpsucker                    43

Courtableau                    River carpsucker                   110

Courtableau                    River carpsucker                   171
Courtableau                    River carpsucker                   393
     The Fausse Pointe Basin, west of the Atchafalaya Basin, is represented
by eight sampling stations.  This area receives water from the Courtableau
area and from  local drainage.  PCB 1254 was detected in two of the water sam-
ples collected from this area, and the mean concentration was 0.90  yg/liter.
It was not detected in any of the bottom sediment samples.

     The Verret Basin, east of the Atchafalaya Basin Floodway, is represented
by 14 stations.  PCB  1254 was found in water at 2 of the 14 stations; however,
it was found only once in bottom sediments.

     The Fordoche area was represented by 13 water- and 12 sediment-sampling
stations.  The mean water content was 0.12 yg/liter, but PCB 1254 was not
detected in any bottom sediments.  The most probable source of this contami-
nation is the  Courtableau area.  Seven fish specimens were collected in
Henderson Lake in the Fordoche area, while 15 were collected in the Courtableau
area.  The mean PCB 1254 fish content was six times greater in the Courtableau
area than the  Fordoche area.


                                   38

-------
     The Atchafalaya Basin Floodway (ABF) had a mean PCB 1254 water concen-
tration of 0.01 yg/liter with a mean station range of 0.00 to 0.14 yg/liter.
Only 6 of 53 water stations sampled (11 percent) resulted in positive detec-
tion of PCB 1254.  PCB's were not detected in any bottom sediment samples
from the area.

     Extensive industrial activities are present in the Bayou Black and Bayou
Chenne areas.  Four stations (H003, H004, H005, H006) are representative of
this industrial area (IA).  The highest mean sediment content~62.4 yg/kg--
was recorded for this area and is approximately two orders of magnitude
greater than the second highest mean bottom sediment concentration for any
of the geographical areas.  PCB 1254 was detected only once in a water sam-
ple from this area.  However, the mean concentration for all water samples
was 0.07 yg/liter which exceeds the EPA water criterion.

     Fifteen water samples, collected from 15 different stations in 5 geo-
graphical areas, contained PCB 1254 above the EPA water quality criterion
of 0.001 yg/liter.  The overall frequency of occurrence was low (4 percent
for water samples and 0.5 percent for sediment samples), and the levels
detected ranged from 0.1 to 5.4 yg/liter.  Station values in excess of the
EPA criterion were single occurrences; none of the stations showed detectable
PCB levels in prior or subsequent sampling.

     The heaviest contamination of PCB's was in the industrial  area southwest
of Morgan City.  In this area, the mean bottom sediment concentration was
62.4 yg/kg, or approximately 100-fold greater than the next highest mean
level recorded.
CHLORDANE


     Because chlordane is a highly persistent chemical,  bioconcentrates  in
aquatic organisms used for human consumption, and is  considered a  human  car-
cinogen, chlordane concentrations should be kept as low  as  feasible.   The
chlordane water quality criterion is 0.001  ppb for the protection  and  prop-
agation of fish and other aquatic life (EPA, 1976).  EPA (1976) cites  several
authors in establishing the range for acute toxicity  of  chlordane  in fresh-
water fish of 5 to 3,000 ppb.   EPA (1976) cites Cardwell et al. (1977) to
demonstrate that fish can concentrate chlordane directly from the  water  by
a factor of 1,000 to 3,000 times and that invertebrate concentrations  may
double the fish estimates.

     Figure 11 details the locations where chlordane  was detected  in fish and
sediment samples in the Atchafalaya Basin.   Chlordane was detected in  7  out
of the 360 bottom sediment samples collected in the study.   The concentrations
ranged from 6 to 30 yg/kg with a mean of 18 yg/kg (Table 11).   The sites where
chlordane was detected .are located in four areas (Courtableau,  Fordoche, West
Atchafalaya Floodway, and the  Buffalo Cove).  Two stations  were located  in
both the Courtableau and the Fordoche areas, and one  station was located in
each of the areas that directly support agriculture or are  directly impacted


                                    39

-------
                                    Atchafalaya Basin, LA.
                                             map area '
                           \  Krotz
                           \ Springs^,
                                               Baton Rouge

                                                5   0   5  10
                                                  Morgan
                                                   City
               /f of Mexico
                                                       Bayou
                                                       Black
Bayou
Chene
                         Atchafalaya
                            River
               Legend
               ^•^^•^••B
               Sediment
Figure 11.   Location  of  stations where chlordane was detected.

                                    40

-------
         TABLE 11.  STATION, DATE, AMD SAMPLE TYPE WHERE CHLORDANE WAS
                    DETECTED IN WATER AND BOTTOM SEDIMENT SAMPLES
                                                    Sample Type
Station
C002
E001
E002
E022
E030
F001
F003
Date
12-17-75
12-15-75
12-15-75
12-12-75
6-13-75
2-15-75
12-12-75
Water
(yg/ liter)
_
-
-
-
ND
ND
ND
Sediment
(yg/kg)
6
32
19
8
9
30
20
ND = Chlordane not detected in sample.
by agricultural drainage.  Chlordane was also detected in  sediment samples
from the Buffalo Cove area.  The mean concentration of the positive chlordane
samples in the Buffalo Cove area was 8.5 yg/kg;  the mean concentration  of the
positive samples from the areas directly affected by agricultural  drainage
was 21.5 pg/kg.  Since there were no other positive chlordane sediment  samples
in any other portion of the Atchafalaya system,  it would appear likely  that
the source of chlordane was from the West Atchafalaya Floodway and the
Courtableau area.

     Chlordane was not detected in any of the water samples.   Also, chlordane
was not detected in any fish specimens.  Chlordane was detected in a spotted
gar collected from Henderson Lake but only at an estimated level  of 5 yg/kg.
Because of the persistence of chlordane in the environment,  its large biocon-
centration factor, and the low frequency of occurrence, it is concluded that
chlordane is not widely distributed in detectable amounts  in  the  water  and
bottom sediments of the Atchafalaya Basin.
ALDRIN-DIELDRIN
     Because dieldrin is a highly persistent chemical,  bioconcentrates  in
aquatic organisms used for human consumption, and is  considered  a  human car-
cinogen, dieldrin concentrations should be kept as low  as  feasible.   The
aldrin-dieldrin water quality criterion is 0.003 ppb  for the  protection and
propagation of fish and other aquatic life (EPA, 1976).  Aquatic organisms
                                   41

-------
metabolically convert aldrin to dieldrin (Gakstatter, 1968).  Because dieldrin
is as toxic or slightly more toxic than aldrin to aquatic organisms (Jensen
and Gaufin, 1966) and because of the metabolic conversion of aldrin to diel-
drin, the acceptable water concentration is based on the presence of either
aldrin or dieldrin.

     Ninety-six-hour LCgQ values for bluegill (Lepomis maaroehirus), fathead

minnow (Pimephalea promelas], and green sunfish (Lepomis cyanellus) are 7.9,
16.0, and 8.5 ppb, respectively, of dieldrin (Tarzwell and Henderson, 1956).
The 20-day LC5Q for Aoroneuria pacifioa (stonefly naiad) was only 0.2 ppb of
dieldrin (Jenson and Gaufin, 1966).

     Biconcentration of aldrin-dieldrin in aquatic organisms (fish, inverte-
brates, and algae) is well documented under laboratory conditions.  In fact,
bioconcentrations of aldrin-dieldrin have been recorded up to 260,000 times
the initial theoretical water concentration (Kawatski and Schmulbach, 1971).

     Figure 12 details the location where aldrin-dieldrin was detected in
fish and bottom sediment samples in the Atchafalaya Basin.  Dieldrin was not
found in water samples and was found in only one bottom sediment sample.  The
one positive dieldrin bottom sediment sample was collected from the lower Old
River and contained 2 yg/kg.  Water movement in this area is restricted to
times when the navigational locks, which connect lower Old River to the
Mississippi River, are open.  However, approximately 66 percent of the fish
specimens examined contained dieldrin (Table 12).  The percentage of positive
aldrin-dieldrin fish specimens did not vary greatly as to geographical area.
Four of the 7 specimens collected in Henderson Lake were positive, while 10
of the 15 specimens were positive in the Courtableau area.  Also, it should
be noted that 11 of the 14 positive samples were below detection limits and
must be treated as unconfirmed results.  Because of the persistence of diel-
drin in the environment, the large bioconcentration factors associated with
dieldrin, and the low levels at which dieldrin was detected in the fish sam-
ples, it is concluded that the fish were exposed to only low levels of aldrin-
dieldrin and that dieldrin is not widely distributed in detectable amounts in
the water and bottom sediments of the Atchafalaya Basin.


DISTRIBUTION PATTERNS


     Two distribution patterns of contaminants in the Atchafalaya Basin are
evident.  The first is associated with industrial activities, and the second
is associated with agricultural activity.

     The heaviest contamination of PCB's was in the industrial area southeast
of Morgan City.  In this area the mean bottom sediment concentration was
62.4 yg/kg or approximately 100-fold greater than the next highest mean bot-
tom sediment level recorded.

     Historically, the construction of levees and canals has improved drainage
in several areas of the Atchafalaya Basin and allowed for the encroachment and

                                   42

-------
                 Old River
Atchafalaya Basin, LA.
           - 31
                                                           Miles
                                                         Kilometers
                                              Baton Rouge

                                               5   0   5  10
           -30
             • Sediment
             *Fish
Figure 12.    Location of the stations  where dieldrin-aldrin was detected.

                                    43

-------
           TABLE 12.   GEOGRAPHICAL  AREA WHERE  DIELDRIN-ALDRIN WAS
                            DETECTED IN FISH SPECIMENS
Geographical
Area
Fordoche
Fordoche
Fordoche
Fordoche
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Courtableau
Species
of Fish
Bowfin
Largemouth bass
Redear sunfish
Spotted gar
Blue catfish
Largemouth bass
River carpsucker
River carpsucker
River carpsucker
River carpsucker
Spotted gar
White crappie
White crappie
White crappie
Concentration
(yg/kg wet weight
Dielann A
2*
11
2*
4*
8*
3*
6*
4*
10
8*
8*
4*
12
7*
), ,
idrin
_
10
-
-
-
-
-
-
-
-
-
-
-
•"
* Unconfirmed result.
                                    44

-------
expansion of agriculture.   These areas—Courtableau,  West Atchafalaya  Floodway,
Fausse Polnte Basin, Morganza Floodway,  and the Fordoche  area, which is  di-
rectly impacted by agricultural  drainage—contained not only  the majority  of
positive pesticide samples but higher levels of concentration than  the other
areas studied.

     The Atchafalaya Basin (below U.S. Highway  190  and  exclusive of the
Fordoche area) and the area south of the Basin  contained  fewer positive  pes-
ticide samples, and those  were generally at lower levels  than in the agri-
culturally influenced areas.

     The pattern of pesticide distribution  that emerges in the Atchafalaya
system reflects the agricultural  activity within or affecting the various
hydrological units.  Hydrological modifications resulting in  lower  water
levels and favoring the encroachment by  or  expansion  of agricultural activi-
ties could be expected to  increase pesticide contamination of the Atchafalaya
system.
                                     45

-------
                                  REFERENCES
Cardwell, R. D., D. G. Foreman, T. R. Payne, and D. R. Wilbur.   1977.
     Acute and chronic toxicity of chlordane to fish and invertebrates.
     EPA-600/3-77-019.  U.S. Environmental Protection Agency, Duluth,
     Minnesota.

Gagliano, S. M., and J. L. van Beek.  1975.  Environmental  base and manage-
     ment study, Atchafalaya Basin, Louisiana.  EPA-600/5-75-006.   U.S.
     Environmental Protection Agency, Washington, D.C.

Gakstatter, J. H.  1968.  Rates of accumulation of   C - dieldrin  residues
     in tissues of goldfish exposed to a single sublethal  dose of    C  -
     aldrin.  J_. Fish. Res. Board Can.  25: 1747.

Goerlitz, D. F., and L. M. Law.  1971.  Note on removal  of sulfur  interfer-
     ences from sediment extracts for pesticide analysis.   Bulletin of
     Environmental Contamination and Toxicology.  6(1):9-10.

Hansen, D. J., and A. J. Wilson, Jr.  1970.  Residues in fish,  wildlife,  and
     estuarines.  Pesticides Monit. Jour.  4:  51.

Jensen, L. D., and A. R. Gaufin.  1966.  Acute and long-term  effects of
     organic insecticides on two species of storefly naiads.   Jour. Water
     Poll. Control Fed.  38:  1273.

Kawatski, J. J., and J. C. Schmulbach.  1971.  Accumulation of insecticide
     in freshwater ostracods exposed continuously to sublethal  concentrations
     of aldrin or  ieldrin.  Trans. Amer. Fish. Soc.  100:   565.

Macek, K. J., and W. A. McAllister.  1970.  Insecticide susceptibility of some
     common fish family representatives.  Trans. Amer. Fish.  Soc.   99:  20.

Nebeker, A. V., F- A. Puglisi, and D. L. DeFoe.  1974.  Effect of  polychlori-
     nated biphenyl compounds on survival and reproduction of the  fathead
     minnow and flagfish.  Trans. Amer. Fish. Soc.  103(3):562.

Nebeker, A. V., and F- A. Puglisi.  1974.  Effect of polychlorinated biphenyls
     (PCB's) on survival and reproduction of Daphnia, Gammarus, and Tanytarsus.
     Trans. Amer. Fish. Soc.  103(4):722.

Reinert, R. E.  1970.  Pesticide concentrations in Great Lakes fish.
     Pesticides Monit. Jour.  3:  233.


                                      46

-------
Sanders, H. 0.  1972.  The toxicities of some insecticides to four species of
     malacostracan Crustacea.  Tech. Paper No.  66.   U.S.  Bureau of Sport
     Fisheries and Wildlife.

Stalling, D. U, and F. L. Mayer, Jr.  1972.   Toxicities  of PCB's to fish and
     environmental residues.  Env. Health Perspectives.   1: 54.

Tarzwell, C. M., and C. Henderson.  1956.  Toxicity of dieldrin to fish.
     Trans. Amer. Fish. Soc.  86:  245.

U.S. Department of the Interior.   1969.   Endrin pollution in the lower
     Mississippi River Basin.  Federal  Water  Pollution Control  Administration,
     Dallas, Texas.

U.S. Environmental Protection Agency.  1976.  Quality  Criteria  for Water.
     EPA 440 Office of Water and  Hazardous Materials,  Washington,  D.C.   501  pp.
                                     47

-------
                                 APPENDIX A
     This appendix summarizes the results of all  water and bottom sediment
samples collected in the Atchafalaya Basin and analyzed for pesticides  and
PCB's (reported as PCB 1254).  It is organized by site-date combinations.
When a specific compound was detected, it was listed under the appropriate
class of compounds and its concentration recorded.  The symbol ND indicates
compounds not detected, while an asterisk (*) signifies that the sample was
not analyzed for PCB's.

     The specific compounds analyzed for their minimum detection levels when
treatment method A was employed are listed in Tables 2 and 3 in the text.
Treatment method A refers to analysis without initial Florisil cleanup;
treatment method B refers to analysis subsequent to Florisil cleanup.
                                     48

-------
                                            APPENDIX A

Sampling
Station
AOOl
AOOl •
AOOl
AOOl
A002
A002
> A002
A002
A002
A003
A004
A004
A004
A004
A004
B001


Treatment
Date Method
11/18/74
12/09/74
01/14/75
06/10/75
11/18/74
12/09/74
01/14/75
04/17/75
06/10/75
01/14/75
11/18/74
12/09/74
01/14/75
06/10/75
12/18/75
11/18/74
A*
A
A
A
A*
A
A
A
A
A
A*
A
A
A

A*
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

ND

Organo-
phosphorus
Compounds
(wg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (ug/kg)
_
- - -
_
_
_
_
_ _
_
- -
_
A* ND ND
A* ND ND
-
A ND ND
B Dieldrin=2.0 ND
p,p1-DDE=7.7
p,p1-TDE=13.4
-
(Continued)

-------
                                          APPENDIX A (Continued)
en
o
Sampling
Station
BOOl
BOOl
BOOl
C001
C001
, C001
C001
C001
C001
C002
C002
C002
C002



Treatment
Date Method
12/09/74
01/14/75
04/17/75
11/18/74
12/09/74
01/14/75
04/14/75
06/10/75
12/17/75
11/18/74
12/09/74
01/14/75
12/17/75

A
A
A
A*
A
A
B
A
-
A*
A
A
-

Water Samples
Organo-
chlorine
Compounds
(vg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
-

Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
-

Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (vg/kg)
_
_
-
A* p,p1-DDE=50
p,p1-TDE=40
-
-
A ND
A p,px-DDE=30
p,px-TED=20
A p.p'-DDE^S
p,pi-TDE=50
A* p,p1-DDE=30
p,p1-TDE=40
-
-
B p,p1-DDE=14.4
p.p'-TOE-lT.S
Tech Chlordane=6
-
-
-
ND
-
-
ND
ND
ND
ND
-
-
ND
.4
    (Continued)

-------
                                         APPENDIX A (Continued)
Ui
Sampling
Station
coos
C003
C003
C004
C004
C004
C004
C004
C004
C004
C004
C005
COOS
COOS


Treatment
Date Method
11/18/74
12/09/74
01/15/75
11/19/74
12/09/74
01/15/75
04/17/75
04/14/75
06/11/75
09/01/76
09/30/76
11/19/74
12/09/74
01/15/75
A*
A
A
A*
A
A
-
A
A
A
B
A*
A
A
Water Samples
Organo-
chlorine
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
PCB 1254=0.1
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (ug/kg) (ug/kg)
A* p,pl-DDE=20
p,p1-TDE=30
-
-
A* ND
A* p,p1-DDE=30
p,p1-TDE=20
-
A p,p1-DDE=10
p,pl-TDE=10
-
A p,p1-DDE=20
A p,p1-DDE=10
A p,p1-TDE=40
p,pl-DDE=30
A* ND
A* ND
- -
ND
-
-
ND

-
ND
-
ND
ND
ND
ND
ND
-
     (Continued)

-------
                                           APPENDIX A (Continued)
en
ro
Sampling
Station
C005
coos
C007
C007
C007
C007
coos
coos
coos
coos
coos
D001


Treatment
Date Method
04/14/75
06/11/75
11/29/76
03/08/77
04/19/77
05/24/77
09/01/76
09/30/76
11/29/76
04/19/77
05/24/77
11/19/74
A
A
A
B
B
B
-
A
A
B
B
A*
Water Samples
Organo-
chlorine
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/ liter)
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
Organo-
chlorine
Treatment Compounds
Method (ug/kg)
A ND
A p,p1-DDE=10
A p,p1-DDE=20
-
B p,p1-DDE=10
- -
A p,p1-DDE=30
p,p1-TDE=20
A p,p1-DDT=20
p,p1-DDE=80
p.p^TDE-SO
A p,p1-DDE=90
p,p1-TDE=50
B p,p1-DDE=20
p,p1-TDE=20
B p,p1-DDE=60
p,p1-TDE=40
_ _
Organo-
phosphorus
Compounds
(yg/kg)
ND
ND
ND
-
ND
-
ND
ND
ND
ND
ND
-
      (Continued)

-------
                                            APPENDIX A (Continued)
en
CO
Sampling
Station
DOOl
DOOl ,(
DOOl •
DOOl
E001
E001
E001
E001
E001
E001
E001
E001


Treatment
Date Method
12/09/74
01/14/75
04/14/75
06/11/75
03/19/74
05/28/74
11/15/74
11/18/74
12/09/74
01/15/75
04/14/75
06/11/75
A
A
A
-
A
A
A*
A
A
-
A
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
-
ND
ND
ND
-
ND
ND
ND
ND

Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
-
ND
ND
ND
-
ND
ND
ND
ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
_
_
A p,p1-DDE=10 ND
p,p1-DDE=10
A p,p1-DDE=10 ND
_ _ _
A p.p^DDE-SO ND
p,pi-DDT=40
p,px-TDE=60
A* p,px-DDE=10 ND
A* ND ND
A* p,p1-DDE=50 ND
p,pl-DDT=70
p,pl-TDE=120
-
A p,p1-DDT=60.0 ND
p.p'-DDE^lO.O
p,pl-TDE=30
- - -
       (Continued)

-------
                                          APPENDIX A (Continued)
en
•£»
Sampling
Stati on
EOOl
E002
E002
E002
E002
E002
E002
E002

E002
E003
Water Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Date Method (yg/Hter) (ug/Hter)
12/18/75 -
03/19/74 A ND ND
05/28/74 A ND ND
11/15/74 A* ND ND
12/09/74 A ND ND
01/15/75 A ND ND
06/11/75 A PCB 1254=1.3 ND
12/15/75 - -

03/08/77 -
11/14/74 A* ND ND
Bottom Sediment Samples
Organo- Organo-
chloMne phosphorus
Treatment Compounds Compounds
Method (yg/kg) (vg/kg)
B Tech. Chlordane=31
p,p1-DDE=48.5
p,p1-DDT=90.0
p,px-TDE=78.7
_
A p,p1-DDE=90
p,pl-TDE=50
A p,p1-DDE=120
p,pi-TDE=90
- _ '
-
A p,p1-DDE=60.0
p,p1-TDE=40.0
B Tech. Chlordane=19
p,p1-DDT=6.0
p,p1-DDE=34.3
p,p1-TDE=27.7
B p,pl-DDE=20
p,pi-TDE=10
A* p.p^DDE^SO
P,P1-TDE=50
.9
-
ND
ND
-
-
ND
.3


ND
     (Continued)

-------
                                         APPENDIX A (Continued)
Sampling
Station
E003 v
E003
E003
E003
Date
12/09/74
01/15/75
06/11/75
12/15/75

Treatment
Method
A
A
A
-
Water Samples
Organo-
chlorine
Compounds
(ug/liter)
ND
ND
ND
-
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
-
Treatment
Method
A*
-
A
A
Organo-
chlorine
Compounds
(ug/kg)
p,pl-DDE=60
p.p'-TDE'SO
-
p,p1-DDE=30
p,p1-DDT=4.9
Organo-
phosphorus
Compounds
(ug/kg)
ND
•mm fl
ND
ND
en
en
                                                                                       p,p1-DDE=43.5

                                                                                       p,pl-TDE=5.6
E004
E004
E004
E004
E004
E005
E005
E005
11/19/74
01/22/75
04/17/75
06/11/75
12/15/75
11/19/74
01/22/75
06/12/75
A*
A
A
A
—
A*
A
A
ND
ND
ND
PCB 1254=2.8
—
ND
ND
ND
ND
ND
ND
ND
—
ND
ND
ND.
A*
-
A
A
B
A*
-
A
p,pl-DDE=70
P,P1-TDE=70
-
ND
p,p1-DDE=100.0
p,pl-TDE=40.0
P,P1DDE=50.0
p,p1-TDE=28.6
p,p1-DDE=60
p,p1-TDE=60
-
p,px-DDE=30
ND
-
ND
ND
ND
ND
-
ND
     (Continued)

-------
                                          APPENDIX A (Continued)
01
Sampling
Station
E006
E006
E006
E006
E006
E007
E007
E008
E008
E008
E008
.E008
E008
E009
E009
E009


Treatment
Date Method
11/19/74
01/23/75
04/17/75
06/12/75
12/15/75
01/23/75
12/15/75
03/19/74
05/28/74
11/15/74
01/23/75
04/17/75
06/12/75
11/19/74
01/23/75
06/12/75
A*
A
A
A
-
A
-
A
A
A*
A
B
A
A*
A
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
-
ND
-
ND
ND
ND
ND
PCS 1254=0.1
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
-
ND
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo-
chlorine
Treatment Compounds
Method (yg/kg)
A*
-
-
A
A
-
A
-
A
A*
-
A
A
A*
-
A
p,p1-DDE=30
p,p1-TDE=40
-
-
p,px-DDE=20
ND
-
ND
-
ND
ND
-
ND
ND
p,p1-DDE=30
p,pl-TDE=40
-
ND
Organo-
phosphorus
Compounds
(yg/kg)
ND
-
-
ND
ND
-
ND
-
ND
ND
-
ND
ND
ND
-
ND
     (Continued)

-------
                                         APPENDIX A  (Continued)
en
Sampling
Station
E009
E010 '
E010
E010
E010
E011
E011
E011
E011
E011
E011
E011
E011
EQ12
E013
E013


Treatment
Date Method
12/15/75
11/19/74
01/23/75
04/17/75
12/15/75
11/19/74
01/23/75
06/12/75
09/01/76
10/26/76
11/29/76
03/09/77
04/20/77
01/23/76
11/19/76
01/15/75
-
A*
A
B
-
A*
A
A
A*
A
A
B
B
A
A*
A
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
-
ND
ND
PCB 1254=1.2
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/liter)
-
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (ug/kg) (wg/kg)
A ND
A* ND
-
A ND
A ND
-
_
B p,p1-DDE=2.7
p,px-TDE=3.8
-
A ND
A ND
B ND
B ND
-
A* p,p1-DDE=10
p,p1-TDE=30
- _
ND
ND
-
ND
ND
-
-
ND
-
ND
ND
ND
ND
-
ND
-
     (Continued)

-------
                                          APPENDIX A (Continued)
en
oo
Sampling
Station
E013
E013
E013
E014
E015
E015
E015
E016
E016
E016
E016
E017
E017
E017
E017
E018
E018
E019


Treatment
Date Method
04/17/75
06/11/75
12/17/75
01/23/75
11/19/74
01/23/75
06/12/75
11/20/74
01/23/75
06/11/75
12/10/75
11/20/74
01/23/75
06/12/75
12/10/75
01/23/75
12/10/75
03/19/74
A
A
-
A
A*
A
A
A*
A
A
-
A*
A
A
-
A
-
A
Water Samples
Organo-
chlorine
Compounds
dig/liter)
ND
PCS 1254=0.6
-
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
-
ND
-
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
-
ND
-
ND
Treatment
Method
A
B
A
-
A*
-
A
-
-
A
A
-
-
A
A
-
A
-
Organo-
chlorine
Compounds
(yg/kg)
ND
ND
ND
-
ND
-
ND
-
-
ND
ND
-
-
ND
ND
-
ND
-
Organo-
phosphorus
Compounds
(yg/kg)
ND
ND
ND
-
ND
-
ND
-
-
ND
ND
-
-
ND
ND
-
ND
-
     (Continued)

-------
                                    APPENDIX A (Continued)
Sampling
Station
E019
E019
E019
E019
E020
E020
S E020
E020
E020
E021
E021
E021
E022

E023
E023
E024


Treatment
Date Method
05/28/74
04/17/75
06/12/75
12/10/75
03/19/74
05/28/74
11/15/74
04/16/75
06/11/75
11/20/74
01/16/75
12/12/75
12/12/75

11/20/74
01/16/75
11/20/74
A
A
A
-
A
A
A
A
A
A
A
-
-

A*
A
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
-
-

ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
-
-

ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
A
A
A
A
-
A
A*
-
A
A*
-
A
B

A*
-
A*
ND
ND
ND
ND
-
ND
p,p1-DDE=10
p,p1-TDE=10
-
ND
ND
-
ND
P,P1-DDE=6.7
p,pl-TDE=9.7
Tech. Chlordane=7.
ND
-
ND
ND
ND
ND -
ND
-
ND
ND
-
ND
ND
-
ND
ND
6
ND
-
ND
(Continued)

-------
                                    APPENDIX A (Continued)
Sampling
Station
E024
E024
1025
1025
E025
E025
1026
1026
1026
1027
1027
1027
1027
1027
1027
1028
1028


Treatment
Date Method
01/16/75
12/12/75
11/20/74
01/16/75
06/13/75
12/12/75
11/20/74
01/16/75
12/12/76
03/19/74
05/28/74
11/15/74
01/16/75
06/13/75
12/12/75
11/20/74
01/16/75
A
-
A*
A
A
-
A*
A
-
A
A
A*
A
A
-
A*
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
-
ND
ND
PCS 1254=0.6
-
ND
ND
-
ND
ND
ND
ND
ND
-
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
{yg/liter}
ND
-
ND
ND
ND
-
ND
ND
-
ND
ND
ND
ND
ND
-
ND
ND
Treatment
Method
-
A
A*
-
A
A
A*
-
A
-
A
A*
-
A
A
-
-
Organo-
chlorine
Compounds
(yg/kg)
-
ND
p,px-DDl=20
p,p1-TDE=40
-
ND
ND
ND
ND
ND
-
ND
ND
-
ND
ND
-
-
Organo-
phosphorus
Compounds
(yg/kg)
-
ND
ND
-
ND
ND
ND
ND
ND
-
ND
ND
-
ND
ND
-
-
(Continued)

-------
                                         APPENDIX A  (.Continued)
CTl
Sampling
Station
E028
E028
E029
E030
E030
E030
E031
E031
E031
E031
E032
E032
E032
E032
E032
E032


Treatment
Date Method
04/16/75
06/13/75
01/16/75
01/16/75
06/13/75
12/12/75
11/20/74
01/16/75
06/13/75
12/12/75
03/19/74
05/25/74
11/15/74
01/20/75
04/16/75
06/13/75
A
A
A
A
A
-
A*
A
A
-
A
A
A*
A
A
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
-
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
-
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
-
A ND
-
-
B Tech. Chlordane=8.
p,p1-DDE=3.7
p,p1-TDE=8.1
A ND
A* p,p1-DDE=20
p,p1-TDE=20
-
A ND
A ND
-
A ND
A* ND
-
-
A ND
-
ND
-
-
8
ND
ND
ND
-
ND
ND
-
ND
ND
-
-
ND
    (Continued)

-------
                                          APPENDIX A (Continued)
CTl
ro
Sampling
Station
E032
E033
E033
E033
E034
E034
E034
E034
E034
E035
E035
E035
E035
E036
E036
E036
E036
E037


Treatment
Date Method
12/12/75
01/20/75
06/13/75
12/12/75
11/20/74
12/12/75
01/20/75
06/13/75
12/18/75
11/20/74
01/20/75
04/16/75
06/13/75
11/21/74
01/21/75
04/16/75
12/17/75
01/21/75
-
A
A
-
A*
-
A
A
-
A*
A
A
A
A*
A
A
-
A
Water Samples
Orga no-
chlorine
Compounds
(yg/liter)
-
ND
ND
-
ND
-
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
-
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(vig/liter)
-
ND
ND
-
ND
-
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
-
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (wgAg) (ng/kg)
A
-
A
B
-
A
-
A
A
A*
-
-
A
A*
-
-
A
-
ND
ND
ND
p,p1-DDE=7.4
-
ND
-
ND
ND
ND
-
-
ND
ND
-
-
ND
-
ND
ND
ND
ND
-
ND
-
ND
ND
ND
-
-
ND
ND
-
-
ND
-
      (Continued)

-------
                                         APPENDIX A (Continued)
CO
Sampling
Station
E038
E038
E038
E039
E039
E040
E040
E040
E041
E041
E041
E041
E042
E042
E042
E043
E043
E043


Treatment
Date Method
01/20/75
04/18/75
12/17/75
01/18/75
12/16/75
03/19/74
05/28/74
04/18/75
11/21/74
01/21/75
04/18/75
12/16/75
03/19/74
01/21/75
12/16/75
03/19/74
05/28/74
11/15/74
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A*
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (ug/kg)
_
_
_
- -
_
_
A ND ND
_
A* ND ND
_
-
A ND ND
-
-
A ND ND
-
A ND ND
A* ND ND
     (Continued)

-------
                                    APPENDIX A  (Continued)
Sampling
Statioji
E043
E043
E043
E044
E044
E044
2 E044
E045
E045
E045
E046
E046
E046
E047
•:r,E047
E047
E047
E047


Treatment
Date Method
01/21/75
04/16/75
12/16/75
11/21/74
01/21/75
04/18/75
12/16/75
11/21/74
01/21/75
12/16/75
11/21/74
01/21/75
12/16/75
03/19/74
05/28/74
11/14/74
01/21/75
04/16/75
A
A
A
A*
A
B
A
A*
A
A
A
A
A
A
A
A
A
B
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
PCB 1254=0.10
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
PCB 1254=0.1
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
-
-
A
A*
-
-
B
-
-
A
A*
-
A
-
A
A*
-
-
_
_
ND ND
ND ND
_
_
p,p1-DDE=4.2 ND
_
_
ND ND
ND ND
_
ND ND
_
ND ND
ND ND
_
_
(Continued)

-------
                                          APPENDIX A (Continued)
cn
Sampling
Station
E047
E048
E048
E048
E048
E049
E049
E049
E050
E050
E050
E050
E050
E051
E051
E051
E051


Treatment
Date Method
12/16/75
03/19/74
11/15/74
01/20/75
12/15/75
01/20/75
04/18/75
12/15/75
03/19/74
11/14/74
01/20/75
04/18/75
12/15/75
03/19/74
05/28/74
11/14/74
01/18/75
A
A
A*
A
A
B
A
A
A
A*
A
A
A
A
A
A*
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
PCB 1254=0.1
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (ug/kg) (ug/kg)
-
-
A
A
-
A*
-
A
A
-
A
A*
-
-
-
p,p1-TDE=10.0
ND
-
ND
-
p.p'-TDE^O.O
ND
-
ND
ND
-
-
-
ND
ND
-
ND
-
ND
ND
-
ND
ND
-
      (Continued)

-------
                                         APPENDIX A (.Continued)
en
en
Sampling
Station
E051
E051
E052
E052
E052
E052
E053
E053
E053
E053
E054
E054
E054
E054
E055
E055
E056


Treatment
Date Method
04/15/75
06/14/75
11/21/74
01/20/75
04/18/75
12/15/75
11/22/74
01/20/75
04/18/75
12/16/75
11/22/74
01/20/75
04/16/75
12/16/75
01/21/75
12/16/75
11/22/74
A
A
A*
A
A
A
A*
B
A
A
A*
B
A
A
B
A
A*
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
PCB 1254=0.7
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND

Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
A p,p1-TDE=10.0 ND
A ND ND
_
_
- - -
A ND ND
A* ND ND
_
_
A ND ND
A* ND ND
_
- - -
- -
_ _
A ND ND
A* ND ND
    (Continued)

-------
                                    APPENDIX A (.Continued)
Sampling
Station
E056
E056
E056
E057
E057
E057
E057
E058
E058
E058
E058
E059
E059
E059
E059
E059
E060


Treatment
Date Method
01/21/75
04/18/75
12/16/75
11/22/74
01/20/75
04/18/75
12/16/75
11/22/74
01/20/75
04/16/75
12/16/75
11/22/74
12/11/74
01/20/74
04/15/75
06/14/75
11/21/74
B
A
A
A*
B
A
A
A*
B
A
A
A*
A
A
A
A
A*
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Treatment
Method
-
-
A
A*
-
-
A
A*
-
A
A
-
-
-
A
A
-
Organo-
chlorine
Compounds
(yg/kg)
-
-
ND
ND
-
-
ND
ND
-
ND
ND
-
-
-
ND
ND
-
Organo-
phosphorus
Compounds
(ug/kg)
-
-
ND
ND
-
-
ND
ND
-
"D
:ID
-
-
-
ND
ND
-
(Continued)

-------
                                         APPENDIX A (.Continued)
CTl
Sampling
Station
E061
E061
E062
E062
E062
E063
E063
E063
E063
E063
E064
E065
E065
E072
E072
E072
E072


Treatment
Date Method
01/25/75
12/15/75
01/18/75
04/15/75
06/14/75
12/11/74
01/18/75
04/18/75
12/15/75
12/16/75
01/18/75
01/18/75
04/18/75
09/01/76
10/26/76
11/29/76
03/09/77
A
A
A
A
A
A
A
A
A
-
A
A
A
A
A
-
B
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
-
ND

Organo-
phosphorus
Compounds
(vg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
-
ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (vg/kg)
-
- -
_
_
_
A* ND ND
_
_
_
A ND ND
_
_
_
A ND ND
A ND ND
A ND ND
B ND ND
    (Continued)

-------
                                         APPENDIX A  (Continued)
CTl
10
Sampling
Station
E072
E072
F001
F001
F001
F001
F001
F002
F002
F002
F003
F003
F003


Treatment
Date Method
04/20/77
05/25/77
11/19/74
12/09/74
01/15/75
06/11/75
12/15/75
01/23/75
12/15/75
12/17/75
11/19/74
01/23/75
04/12/75
B
B
A*
A
A
A
A
B
A
-
A*
B
A
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND

Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
-
ND
ND
ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
B ND ND
B ND ND
A* p,pl-DDE=0.100 ND
p,p1-TDE=0.80
A* p,p1-DDE=20 ND
p,px-TDE=10
_
A ND -
B p,pl-DDE=101.9 ND
p,pl-TDE=58.6
p,p1-DDT=71.3
Tech. Chlordane=29.6
-
_
A ND ND
A* p,pl-DDE=70 ND
p.p^TDE^LO
-
- - -
     (Continued)

-------
                                    APPENDIX A  (Continued)
Sampling
Station
F003
F003
F004
F004
F004
F004
F004
F005
F005
F005
F005
F005
F005
Date
06/12/75
12/12/75
11/20/74
01/20/75
04/16/75
06/13/75
06/14/75
03/19/74
05/28/74
11/15/74
01/20/75
06/13/75
12/12/75

Treatment
Method
A
A
A*
B
A
A
A
A
A
A*
A
A
I
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
PCB 1254=1.8
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (ng/kg) (wg/kg)
A ND
B p,pJ-DDE=63.9
p,p1-TDE=75.1
p,p1-DDT=39.6
Tech. Chlordane=20
-
-
-
A ND
-
-
A ND
A* p,p1-DDE=20
p,pl-TDE=20
-
A p,p1-DDE=10.0
p.p^TDEKLO.O
A p,p1-DDE=10.0
p,p1-TDE=10.0
ND
ND
.03
-
-
-
ND
-
-
ND
ND
-
-
ND
(Continued)

-------
                                    APPENDIX A (Continued)
Sampling
Station
F006
F006
F006
F006
F006
F006
F007
F007
F007
F008
F008
F008
F008
F009
F009
F009


Treatment
Date Method
03/19/74
11/15/75
01/20/75
04/16/75
06/13/75
12/12/75
11/21/74
01/20/75
12/12/75
11/21/74
01/20/75
06/14/75
12/12/75
11/21/74
04/16/75
06/14/75
A
A*
A
A
A
A
A*
A
A
A*
A
A
A
A*
A
A
Water Samples
Organo-
chlorine
Compounds
(ug/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
PCB 1254=5.40
Bottom Sediment Samp!
Organo-
, phosphorus
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
es
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (ug/kg)
-
A*
-
-
A
A
A*
-
A
A*
-
A
A
A*
-
A
-
ND
-
-
ND
ND
ND
-
ND
ND
-
p,p1-DDE=10.0
p,pl-TDE=10.0
ND
ND
ND
ND
-
ND
-
-
ND
ND
ND
-
ND
ND
-
ND
ND
ND
ND
ND
(Continued)

-------
                                         APPENDIX A  (Continued)
ro
Water Samples
Sampling
Station
F009
F010

FOll
FOll
FOll
FOll
FOll
FOll
G001
G001
G001
Treatment
Date Method
12/12/75
12/17/75

09/01/76
09/30/76
11/29/76
03/08/77
04/19/77
05/24/77
03/19/74
11/20/74
01/22/75
A
-

A
A
A
B
B
B
A
A*
A
Organo-
chlorine
Compounds
(yg/liter)
ND
-

ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo-
phosphorus
Compounds
(yg/liter)
ND
-

ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
B p,pl-DDE=2.9
p,p1-TDE=3.5
B p,pl-DDT=7.1
p,p1-TDE=10.8
A p,p1-DDE=30
p,p1-TDE=20
A p,p1-DDE=50
p,plTDE=40
A p,px-DDE=90
p,p1-TDE=50
B p,pl-DDE=10
p,p1-TDE=10
B p,p1-DDE=20
p,pl-TDE=20
B p,pl-DDE=30
p,p1-TDE=10
-
A* ND
-
ND


ND
ND
ND
ND
ND
ND
-
ND
-
     (Continued)

-------
                                         APPENDIX A (Continued)
CO
Sampling
Station
GOOl
GOOl
G002
G002
G002
G002
G002
G003
G003
G003
G003
G003
G003
G004
G004
G004
G005


Treatment
Date Method
06/11/75
12/12/75
03/19/74
11/15/74
01/21/75
06/11/74
12/12/75
03/19/74
05/23/74
11/15/74
01/21/75
04/15/75
12/12/75
11/20/74
01/21/74
12/11/75
05/28/74
A
A
A
A*
A
A
A
A
A
A*
A
A
A
A*
A
A
A
Mater Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Treatment
Method
A
A
-
A*
-
A
A
-
A
A*
-
A
A
A*
-
A
A
Organo-
chlorine
Compounds
dig/kg)
ND
ND
-
ND
-
ND
ND
-
ND
ND
-
ND.
ND
ND
-
ND
ND
Organo-
phosphorus
Compounds
(ug/kg)
ND
ND
-
ND
-
ND
ND
-
ND
ND
-
ND
ND
ND
-
ND
ND
     (Continued)

-------
                                    APPENDIX A  (Continued)
Sampling
Station
G005
G005
G005
G005
G006
G006
G006
G006
G006
G007
G007
G007
G007
G008
G008
G008
G008


Treatment
Date Method
12/11/74
01/17/75
04/18/75
06/14/75
05/28/74
12/11/74
01/18/75
04/15/75
06/14/75
05/28/74
12/11/74
01/18/75
12/11/75
03/19/74
11/14/74
12/11/74
01/18/75
A
A
A
A
A
B
A
A
A
A
A
A
A
A
A*
B
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
ND
ND
ND
ND
ND
PCB 1254=0.7
ND
ND
ND
ND
ND
ND
ND
ND
ND
PCB 1254=0.4
ND
Bottom Sediment Sampl
Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Treatment
Method
A*
_
-
A
A
A*
-
A
A
A
A*
-
A
-
A*
B
-
es
Organo- Organo-
chlorine phosphorus
Compounds Compounds
(yg/kg) (wg/kg)
ND
-
-
p,p1-DDE=20
ND
ND
-
ND
ND
ND
ND
-
ND
-
ND
PCB 1254=30.2
-
ND
-
-
ND
ND
ND
-
ND
ND
ND
ND
-
ND
-
ND
ND
-
(Continued)

-------
                                         APPENDIX A (Continued)
en
Sampling
Station
G008
G008
G009
G009
G009
G009
G009
G009
G009
G010
G010
G010
G011
G011
G011
G012
G012


Treatment
Date Method
06/14/75
12/11/75
03/19/74
05/28/74
11/14/74
01/18/75
04/15/75
12/11/75
12/11/75
01/18/75
06/14/75
12/11/75
01/17/75
04/18/75
06/14/75
11/23/74
12/10/74
A
A
A
A
A*
A
A
A
A
A
A
A
A
A
A
A*
A
Water Samples
Organo-
chlorine
Compounds
(pg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Sampl
Organo-
phosphorus
Compounds
(ug/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
es
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (yg/kg)
A
A
-
A
-
-
A
A
A
-
A
A
-
A
A
-
—
ND
ND
-
ND
-
-
ND
ND
ND
-
ND
ND
-
p,p1-DDE=10.0
ND
-
-
ND
ND
-
ND
-
-
ND
ND
ND
-
ND
ND
-
ND
ND
-
-
     (Continued)

-------
                                    APPENDIX A  (Continued)
Sampling
Station
GO 12
G012
G012
G013
G013
G01A
G014
G014
G014
G014
G014
H001
H001
H002
H002
H003
H003
H003


Treatment
Date Method
12/11/74
01/17/75
06/14/75
01/17/75
04/18/75
11/23/74
12/10/74
01/17/75
04/18/75
06/14/75
12/17/75
01/18/75
12/11/75
01/17/75
12/11/75
11/23/74
12/10/74
01/17/75
-
A
A
A
A
A*
A
A
A
A
A
A
A
B
A
A*
A
A
Water Samples
Organo-
chlorine
Compounds
(yg/liter)
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (yg/kg) (vg/kg)
A*
.
B
-
A
-
A*
-
A
A
A
-
-
-
A
-
A*
-
ND
-
ND
-
p,pi-DDE=10.0
-
ND
-
p,p1-DDE=10.0
ND
ND
-
-
-
ND
-
ND
-
ND
-
ND
-
ND
-
ND
-
ND
ND
ND
-
-
-
ND
-
ND
-
(Continued)

-------
                                    APPENDIX A (Continued)
Sampling
Station
H003
H003
H003
H004
H004
H004
j H004
H004
H005
H005
H005
H005
H005
H005
H005
H006
H006
• - -

Treatment
Date Method
04/15/75
06/14/75
12/11/75
11/23/74
12/10/74
01/17/75
06/14/75
12/11/75
03/19/74
05/28/74
11/14/74
12/10/74
01/17/75
04/15/75
06/14/75
03/19/74
11/14/74
A
A
A
A*
A
A
A
A
A
A
A*
A
A
A
A
A
A*
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
PCB 1254=1.6
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(yg/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Treatment
Method
A
-
A
-
A*
-
A
B
-
A
-
A*
-
A
B
-
A* .
Organo- Organo-
chlorine phosphorus
Compounds Compounds
(yg/kg) (yg/kg)
PCB 1254=140.0
-
PCB 1254=220.0
-
ND -
-
ND
PCB 1254=41.4
-
ND
-
ND
-
ND
PCB 1254=68.1
-
ND
ND
-
ND
-
ND
-
ND
-
-
ND
-
ND
-
ND
ND
-
ND
(Continued)

-------
                                          APPENDIX A (Continued)
oo
Sampling
Station
H006
H006
H006
H006
H006
H007
H007
H007
H007
H007
H007
H008
H008
H008
H008
H009
H009


Treatment
Date Method
12/10/74
01/17/75
06/14/75
12/11/75
12/11/76
11/23/74
12/10/74
01/17/75
04/15/75
06/14/75
12/11/75
11/22/74
12/10/74
01/17/75
12/11/75
11/22/74
12/10/74
A
A
A
A
-
A*
A
A
A
A
A
A*
A
A
A
A*
A
Water Samples
Organo-
chlorine
Compounds
(vg/ liter)
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ng/ liter)
ND
ND
ND
ND
-
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Organo- Organo-
chlorine phosphorus
Treatment Compounds Compounds
Method (ng/kg) (wg/kg)
A*
-
B
-
A
-
A*
-
A
A
A
-
-
-
A
-
A*
ND
-
PCB 1254=52.6
-
ND
-
ND
-
p,pl-TDE=10.0
ND
ND
-
-
-
ND
-
ND
ND
-
ND
-
ND
-
ND
-
ND
ND
ND
-
-
-
ND
-
ND
      (Continued)

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                                    APPENDIX A (Continued)
Sampling
Station
H009
H009
H009
H010
H010
H010
H011
H011
H011
H011
H012
H012
H012
H012
H013
H013
HO 13
HO 13
Date
01/17/75
04/15/75
12/11/75
12/10/74
01/17/75
12/11/75
11/22/74
12/10/74
01/17/75
12/11/75
11/22/74
12/10/74
01/17/75
04/15/75
11/22/75
12/10/74
01/17/75
12/11/75

Treatment
Method
A
- A
A
A
A
A
A*
A
A
A
A
A
A
A
A*
A
A
A
Water Samples
Organo-
chlorine
Compounds
(yg/ liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/liter)
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Treatment
Method
-
A
A
A*
-
A
-
A*
-
A
-
A*
-
A
A*
-
-
A
Organo-
chlorine
Compounds
(yg/kg)
-
ND
ND
ND
-
ND
-
ND
-
ND
-
ND
-
ND
ND
-
-
ND
Organo-
phosphorus
Compounds
(yg/kg)
-
ND
ND
ND
-
ND
-
ND
-
ND
-
ND
-
ND
ND
-
-
ND
(Continued)

-------
                                       APPENDIX A  (Continued)
Sampl 1 ng
Station
H014

Treatment
Date Method
12/11/75 A
Water Samples
Organo-
chlorine
Compounds
(wg/ liter)
ND
Bottom Sediment Samples
Organo-
phosphorus
Compounds
(ug/ liter)
ND
Organo-
chlorine
Treatment Compounds
Method (pg/kg)
_ _
Organo-
phosphorus
Compounds
(pg/kg)
_
00
o

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                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before completing}
1. REPORT NO.
   EPA-600/4-79-061
                              2.
                                       3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
PESTICIDES AND POLYCHLORINATED  BIPHENYLS
IN THE  ATCHAFALAYA BASIN, LOUISIANA
                                                            5. R7.PORT DATE
                                                             September 1979
                                      6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
                                                           8. PERFORMING ORGANIZATION REPORT NO.
Stephen C.  Hern, Victor W.  Lambou,  and Han Tai
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Environmental  Monitoring and  Support Laboratory
Office  of Research and Development
U.S.  Environmental Protection Agency
Las  Vegas, Nevada  89114
                                       10. PROGRAM ELEMENT NO.
                                         1BD613
                                       11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
U.S.  Environmental Protection  Agency--Las Vegas, NV
Environmental Monitoring  and Support Laboratory
Office of Research and Development
Las  Vegas,  Nevada 89114
                                       13. TYPE OF REPORT AND PERIOD COVERED
                                         In-house
                                       14. SPONSORING AGENCY CODE

                                        EPA/600/07
15. SUPPLEMENTARY NOTES
16. ABSTRACT
The collection and analysis of  samples  for pesticide and polychlorinated biphenyls
(PCB's)  were included in the Atchafalaya  River Basin Water  and  Land  Study.
From  1974  to 1977, 743 samples  were  collected from 118 stations in the Atchafalaya
Basin.   Water, bottom sediment,  and  fish  samples were analyzed  for 9 organophosphorus
compounds  and 18 organochlorine  compounds.   No organophosphorus compounds were detected
in any  fish, water, or bottom sediment  samples.  Only a few organochlorine compounds,
i.e., aldrin, dieldrin, PCB's,  chlordane,  and DDT and its derivatives, are present
in bottom  sediments of the Atchafalaya  Basin.  With the exception of PCB's none of
the compounds was detected in water  samples.  Aldrin, dieldrin, PCB's, and DDT
and its  derivatives were found  in  fish  samples.  The pattern of pesticide distribution
that  emerges in the Atchafalaya  Basin  reflects the agricultural  activity within
or affecting the various hydrological  units of the Basin.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                              b.lDENTIFIERS/OPEN ENDED TERMS
                                                       COSATl Field/Group
Pesticides
  DDT
  Aldrin
  Dieldrin
Chlordane
Water pollution
Polychlorinated
  biphenyls
Atchafalaya  Basin
07C
18. DISTRIBUTION STATEMENT
RELEASE TO  PUBLIC
                                              19. SECURITY CLASS (This Report)
                                               UNCLASSIFIED
                                                                         21. NO. OF PAGES
                                                         92
                                              20. SECURITY CLASS (Thispage)
                                               UNCLASSIFIED
                                                    22. PRICE
EPA Form 2220-1 (Rev. 4-77)   PREVIOUS EDITION is OBSOLETE
                                                           U.S. GOVERNMENT PRINTING OFFICE: 1 979-683-O91 /22O5

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