vvEPA
United States
Environmental Protection
Agency
Environmental
Research Laboratory
Narragansett Rl 02882
EPA-600/ 3-78-035
March 1978
Research and Development
Biological Availability
of Pollutants
to Marine Organisms
Ecological Research Series
-------
RESEARCH REPORTING SERIES
Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology Elimination of traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are
1 Environmental Health Effects Research
2 Environmental Protection Technology
3. Ecological Research
4 Environmental Monitoring
5. Socioeconomic Environmental Studies
6. Scientific and Technical Assessment Reports (STAR)
7. Interagency Energy-Environment Research and Development
8. "Special" Reports
9. Miscellaneous Reports
This report has been assigned to the ECOLOGICAL RESEARCH series. This series
describes research on the effects of pollution on humans, plant and animal spe-
cies, and materials. Problems are assessed for their long- and short-term influ-
ences. Investigations include formation, transport, and pathway studies to deter-
mine the fate of pollutants and their effects. This work provides the technical basis
for setting standards to minimize undesirable changes in living organisms in the
aquatic, terrestrial, and atmospheric environments.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.
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EPA-600/3-78-035
March 1978
BIOLOGICAL AVAILABILITY OF
POLLUTANTS TO MARINE ORGANISMS
by
John R. Montgomery, Mary Price, John Thurston,
Gina Lalte de Castro, Luz Leida Cruz and
Domenica DeCaro Zlnmerman
Center for Energy and Environment Research
Mayaguez, Puerto Rico 00708
Contract No. IAG-D-405^1
Project Officer
Gerald Pesch
Environmental Research Laboratory
Narragansett, Rhode Island 02882
ENVIRONMENTAL RESEARCH LABORATORY
OFFICE OF RESEARCH AND DEVELOPMENT
U.S. ENVIRONMENTAL PROTECTION AGENCY
NARRAGANSETT, RHODE ISLAND 02882
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DISCLAIMER
This report has been reviewed by the Narragansett
Environmental Research Laboratory, U.S. Environmental
Protection Agency, and approved for publication. Approval
does not signify that the contents necessarily relfect the
views and policies of the Environmental Protection Agency,
nor does mention of trade names or commerical products
constitute endorsement or recommendation for use.
11
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FOREWORD
The Environmental Research Laboratory of the U.S.
Environmental Protection Agency is located on the shore of
Narragansett Bay, Rhode Island. In order to assure the
protection of marine resources, the laboratory is charged
with providing a scientifically sound basis for Agency
decisions on the environmental safety of various uses of
marine systems. To a great extent, this requires research
on the tolerance of marine organisms and their life stages as
well as of ecosystems to many forms of pollution stress. In
addition, a knowledge of pollutant transport and fate is
needed.
The waters of coastal marine environments, as well as
the inshore sediments, often become the ultimate sinks for
pollutants released into them from industrial sources.
Plants and animals in those areas are subjected to varying
concentrations of the contaminants which can be taken up,
transported, and made available to upper trophic levels
including man. Studies as the one reported herein help to
develop and test predictive models on the bioavailability,
uptake, and transport of toxic substances in marine tropical
systems.
This small scale experiment serves as an initial step
to assess the impact of pollutants in larger aquatic
environments.
Eric D. Schneider
Director
Environmental Research Lab./Narragansett^ RI
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ABSTRACT
This research was initiated to determine the rates of
uptake, by a Thalassia testudinum ecosystem, of Cd, Cr, Cu, Ni,
Pb, and Zn which were leached from sewage sludge by sea water.
The_experimental design used aerated flowing sea water (8.4£
min 1), which passed over a 0.1 m3 bed of sewage sludge before
traversing the model ecosystem. The tanks, both control and
experimental, were 9.2 mx 0.9 mx l.lmin size with a volume
of 3.1 x 10^ liters. Each tank contained sand to a depth of
0.5m for a total volume of M-. 2 m^. The experiment ran for 125
days from March 1975 to July 1975 and was duplicated from
December 1975 to April 1976.
The largest net uptake of metals occurred in the "fouling
organisms" where Cd, Pb, and Zn uptake closely paralleled the
net loss of metals from the sewage sludge. Thalassia leaves
showed a net uptake for Cr, Pb, Ni, and Zn for both experiments.
The urchin (Lytechinus variegatus), a herbivore on Thalassia
leaves, also demonstrated a net uptake of Cu, Cr, Pb, Zn, and
Ni in both experiments. The sea cucumber (Holothuria mexicana)
in both experiments showed a net uptake of Cr, Cu, Pb, and Zn./
Net uptake of metals in mangroves (Rhizophora mangle) was limited
to the roots. In the first experiment Ni and Zn showed a
significant uptake whereas in the second experiment only Cr
demonstrated a net uptake. The uptake in mangrove roots appeared
to be a direct function of metal concentration in the sediment.
The lack of apparent metal uptake in the sediment, except for
Ni, Pb, and Zn in the second experiment, was probably due to
the sampling technique rather than to a lack of uptake of metals
by the sediment.
No consistent or significant metal uptake was found for the
clam (Codokia orbicularis), oyster (Crassostrea rhizophora) or
the snail(Nerita tessplata).
The lack of sufficient sample mass, for these organisms,
probably obscured any net uptake that may have occurred.
The results indicate that the dumping of sewage sludge in
coastal tropical waters can lead to the uptake and concentration
of toxic trace metals by members of a turtle grass community.
IV
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CONTENTS
Foreword ii
Abstract iii
Figures v
Tables viii
Acknowledgment ix
1. Introduction 1
2 . Methods 4
Construction of sea water tanks 4
Sewage sludge 5
Sampling 5
organisms 5
overlying water 6
pore water 7
sediment 7
dissolved and particulate trace metals 7
Physical and chemical analyses 7
general 7
oxygen, temperature and salinity 8
dissolved reactive phosphate 8
particulate trace metals 8
soluble trace metals 8
biological and sediment samples 9
3. Results 12
4 . Discussion 16
5 . Conclusions and Recommendations 20
Bibliography 64
Appendices
A. Sorted raw data, Experiment I and II 68
B. Results for net uptake for Experiment I and II 91
C. Experiment II, water data 131
D. Wet/dry ratios for all samples 133
v
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FIGURES
Number Page
1 Study site location 21a
2 Diagram of experimental design, tank configuration,
and sea water system 22
3 Flow chart of trace metal and hydrocarbon sampling
from collection processing into final sample
form 23
4- Dissolved reactive phosphate concentration
Experiment I and II, control 24
5 Dissolved reactive phosphate concentration
Experiment I and II, experimental 25
6 Mean net uptake of Cd between experimental and
control, fouling organisms and sewage-
Experiment 1 26
7 Mean net uptake of Cd between experimental and
control, fouling organisms and sewage-
Experiment II 27
8 Mean net uptake of Cu between experimental and
control, fouling organisms and sewage-
Experiment 1 28
9 Mean net uptake of Pb between experimental and
control, fouling organisms and sewage-
Experiment 1 29
10 Mean net uptake of Pb between experimental and
control, fouling organisms - Experiment II 30
11 Mean net uptake of Zn between experimental and
control, fouling organisms and sewage-
Experiment 1 31
12 Mean net uptake of Zn between experimental and
control, fouling organisms and sewage-
Experiment II 32
13 Mean net uptake of Ni between experimental and
control, fouling organisms and sewage-
Experiment II 33
VI
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1M- Mean net uptake of Cr and Ni between experimental
and control, sewage - Experiment 1 34
15 Mean net uptake of Cr between experimental and
control, fouling organisms and sewage-
Experiment II 35
16 Mean net uptake of Cu between experimental and
control, Thalassia leaves and urchin guts-
Experiment 1 36
17 Mean net uptake of Cu between experimental and
control, Thalassia leaves and urchin guts-
Experiment II 37
18 Mean net uptake of Cr between experimental and
control, Thalassia leaves and urchin guts-
Experiment 1 38
19 Mean net uptake of Cr between experimental and
control, Thalassia leaves and urchin guts-
Experiment II 39
20 Mean net uptake of Pb between experimental and
control, Thalassia leaves and urchin guts-
Experiment 1 40
21 Mean net uptake of Pb between experimental and
control, Thalassia leaves and urchin guts-
Experiment II .41
22 Mean net uptake of Ni between experimental and
control, Thalassia leaves and urchin guts-
Experiment I A2
23 Mean net uptake of Ni between experimental and
control, Thalassia leaves and urchin guts-
Experiment II -43
24- Mean net uptake of Zn between experimental and
control, Thalassia leaves and urchin guts-
Experiment I -44
25 Mean net uptake of Zn between experimental and
control, Thalassia leaves and urchin guts-
Experiment II. . .45
26 Mean net uptake of Cd between experimental and
control, Thalassia leaves and urchin guts-
Experiment II .46
27 Mean net uptake of Ni between experimental and
control, Thalassia roots and mangrove roots-
Experiment I .47
28 Mean net uptake of Ni between experimental and
control, Thalassia roots and mangrove roots-
Experiment II .48
29 Mean net uptake of Cr between experimental and
control, Thalassia roots - Experiment I 49
vii
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30 Mean net uptake of Cr between experimental and
control, Thalassia roots and mangrove roots-
Experiment II 50
31 Mean net uptake of Cu between experimental and
control, Thalassia roots - Experiment 1 51
32 Mean net uptake of Cu between experimental and
control, Thalassia roots and mangrove roots-
Experiment II 52
33 Mean net uptake of Pb between experimental and
control, Thalassia roots - Experiment 1 53
34 Mean net uptake of Pb between experimental and
control, Thalassia roots and mangrove roots-
Experiment II 54
35 Mean net uptake of Zn between experimental and
control, mangrove roots - Experiment 1 55
36 Mean net uptake of Zn between experimental and
control, Thalassia roots and mangrove roots-
Experiment II 56
37 Mean net uptake of Cd between experimental and
control, Thalassia roots - Experiment II 57
38 Mean net uptake of Cr and Ni between experimental
and control, sea cucumber guts - Experiment 1 58
39 Mean net uptake of Cd and Cr between experimental
and control, sea cucumber guts - Experiment II 59
40 Mean net uptake of Cu between experimental and
control, sea cucumber guts - Experiment I .60
41 Mean net uptake of Cu and Ni between experimental and
control, sea cucumber guts - Experiment II £1
42 Mean net uptake of Pb and Zn between experimental and
control, sea cucumber guts - Experiment I .62
43 Mean net uptake of Pb and Zn between experimental and
control, sea urchin guts - Experiment II .63
Vlll
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-TABLES
Number Pa
1 Physical dimensions, number stocked and sample
size of animals, Experiment I and II 5
2 Taxonomic list of experimental organisms 6
3 Results of trace metal analyses of NBS biological
standards, with minimum detectable concentrations... 10
4 Initial trace metal concentrations of sewage sludge,
Experiment I and II 15
IX
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ACKNOWLEDGEMENTS
The authors would like to express their appreciation to
the following organizations: the Puerto Rico Department of
Agriculture for wet laboratory facilities and Harbor Branch
Foundation and the Smithsonian Institution for financial support
and use of facilities for completion of this report. The
technical support of the following people was invaluable for
the completion of this report: Mr. Pedro Acosta, Mr. Jon Cole,
Mr- Jose Echevarria, Mr. Thomas Prinslow, Mr. Jose Ramirez,
Ms. Arlene Ramirez, and Dr. Kenneth Watters. Special thanks
go to Mrs. Barbara Herman, Mrs. Jackie McKay, and Mrs. Rose
Neville for typing the manuscript, and Mr. Tom Smoyer for
photographic aid. Our sincere thanks to Dr- Frank Lowman for
the initial conception of this project.
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SECTION I
INTRODUCTION
The deposition of sewage sludge in the marine environment
may adversely affect the resident organisms in three ways:
(1) Siltation with resultant death of sessile organisms. (2)
Production of an anoxic environment due to the oxidation of
the large organic load in the sludge. (3) By the release of
toxic materials into water in forms that may be incorporated
into the organisms. These materials usually include metals
(zinc, cadmium, chromium, nickel, lead and copper), pesticides
or organohalogens and hydrocarbons of petroleum origin. The
mass dumping of treated sewage sludge into oceans and shallow
inshore areas is occurring in a number of areas. The New York
Bight, is an example of an area receiving large quantities of
solid waste for almost a century (Carmody et al., 1973).
Carmody, Pearce, and Yasso (1973) determined that there was a
definite increase in the sediment of Cr, Cu, Pb, Ni and Zn due
to the dumping of sewage sludge. The concentration of Cr, Cu,
Cd, Pb, Ni, and Zn in sewage sludge is markedly elevated over
ambient marine water concentrations (Salotto, B.V- and Farrell,
J.B., 1971; Jacobs, S.A., 1973). Therefore, the deposits of
this sludge in the marine environment could result in the up-
take and concentration within the food web. The uptake of
metals by marine organisms and concentration within the food
web has been demonstrated numerous times (Jacobs, S.A., 1973;
Kerfoot and Jacobs, 1973a, 1973b; Kerfoot, W.B., 1973; Shuster
and Pringle, 1969; Valiela ejt aJL. , 1974).
The most extensive compilation of metal concentrations in
the tissues of marine organisms is found in Vinogradov (1953)
and Goldberg (1965). However, the results were usually based
on very small sample sizes so it is very difficult to assess
the background levels of metals in marine organisms. Eisler
(1973) compiled an annotated bibliography on biological effects
of metals on aquatic organisms. Montgomery et al. (1976) found
that in order to obtain a valid sample size for trace metal
determination in the thread fin herring (Opisthonema oglinum
Le Sueur)) a pooled sample size of from 50 to 124 fish was
necessary in order to detect a 15 to 25 percent difference in
two populations. Other researchers have shown the effect of
various metals on the marine food web or individual organisms
both in laboratory models and field experiments (Phelps, D.K.
et al., 1975; Nair, K.V. et al., 1973; Eisler, R. et al., 1972;
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Eisler, R. , 1971; Gardner, G.R. and G. LaRoche, 1973; Jackim,
E., 1973; Frazier, J.M., 1976; Ferrell, R.E. et al., 1973;
Huggett, R.J., 1973; Hannan, P.J. and C. Patouillet, 1972).
Schroeder (1975) demonstrated, using radioactive isotopes, that
Thalassia testudinum would concentrate cobalt and manganese in
the leaves rather than the root structure (excluding rhizomes).
The incorporation of cations in Thalassia testudinum was primarily
accomplished through the leaves (Schroeder, 1975).
The use of sewage sludge to amend agricultural soils has
been extensively studied (Street, J.J. ejt aJL. , 1977; Silviera,
D.J. and L.E. Sommers, 1977; Turner, R.E. ejt a^L. , 1976). However,
very little has been published regarding the rates of release
of potentially toxic trace metals into complex tropical eco-
systems. The necessity for this type of controlled field experi-
ments was explained by Menzel (1977) for the CEPEX experiment.
Inherently, it is impossible to duplicate exactly the marine
ecosystem due to its spatial and temporal dimensions. Also,
the isolation of organisms from their surrounding environment
produces results that are ambiguous. Therefore, it is unlikely
that any "artificial" system can duplicate conditions in the
natural environment.
It may not be necessary-to duplicate in a laboratory the
real system, but rather to produce a system that can duplicate
itself (Menzel, D.W., 1977), while at the same time attempting
to duplicate the gross physical marine parameters, such as light,
suspended sediment load, salinity and temperature. In order to
do this, a flow-through sea water system with replicate experi-
ments in different seasons is mandatory.
Our objectives in this research were to use a flow-through
system of sufficient size and complexity as to nearly duplicate
a tropical marine ecosystem and also allow repeatability over
varying seasons. This system would allow us to determine the
rates of uptake, by a tropical marine community, of Cu, Cd, Cr,
Zn, Ni, and Pb which were leached from sewage sludge by sea
water. This community was comprised of turtle grass (Thalassia
testudinum) with its associated sedimental infauna, spiny sea
urchin (Lytechinus variegatus), sea cucumber (Holothurea mexicana),
attached "fouling organisms", plankton and the red mangrove
(Rhizophora mangle).
This ecosystem was chosen for three reasons: (1) the system
comprised a tightly bound community with strong interaction
between the members, (2) the important role of the seagrass/
mangrove ecosystem in the overall tropical near-shore environment
(Jones, J.A., 1968; Odum, H.T. et aJL. , 1959; Golley, F. et al.,
1962), (3) the possibility of the active concentration of these
trace metals in the higher trophic levels with passage of the
materials through the food webs.
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The results from this research will aid in forming a basis
for determining the potential effects of leached toxicants from
sewage sludge on a tropical marine ecosystem. These problems are
especially critical in Puerto Rico. The population density on
the island is nearly 2000 people per square kilometer; about
ten times that of the continental United States. Only a few
towns and cities have sewage treatment facilities and most of
the sewage is dumped, minimally treated, into the rivers and bays.
With the increased awareness of the potential detrimental
effects of raw sewage on the health of the inhabitants and the
marine ecosystem, the construction of sewage plants will increase.
This increase of modern plants will produce large amounts of
sewage sludge. The disposal of this sludge will pose an especially
critical problem in the densely populated, mountainous island of
Puerto Rico as the available land for sludge disposal is very
limited and disposal poses its own set of ecological problems
at least as serious as marine disposal of sludge. Therefore, the
judicious selection of ocean dump sites for sludge, if this
alternative is chosen, will require estimates on the leaching
rates of toxic substances from sewage sludge by sea water. These
substances can enter the food web and become concentrated relative
to the concentration in the water column. The toxicity to marine
organisms of Cd, Pb, Cr, Ni, Zn and Cu has been demonstrated
(Eisler, R., 1971; Eisler, R. et al., 1972; Frazier, J.M., 1976;
Gardner, G. and LaRoche 1973; Ikuta, K., 1968). It is also
possible that some of the leachable substances could promote
growth in portions of the ecosystem, i.e., vitamins, chelators,
organic growth substances (Vallentyne, J.R., 1957). However, the
potential possible benefits of sewage sludge leachate will not
be examined in this research.
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SECTION 2
METHODS
Construction of Sea Water Tanks
The sea water system and tanks were located near Bramadero
Bay, approximately 8 km from Mayaguez, Puerto Rico (Fig. 1).
Two tanks (9.2 m x 0.9 m x 1.1 m) were constructed from 1.9 cm
exterior grade plywood with 5 cm x 10 cm external framing (see
Fig. 2). These tanks were sealed with two coats of fibreglass
epoxy resin and leached in running sea water for 2 weeks. The
tanks were located in an open structure. The roof of the structure
was fabricated of galvanized corrugated roofing. The underside
of the roof was covered with plastic sheeting to prevent zinc
contamination.
Flowing Sea Water System
3
Each tank was filled with 4.2 m of calcareous beach sand
(7% CaCO,.) from Aguada, Puerto Rico. The mean depth of sand'was
0.5 m with an additional 0.1 m of sand over the last 3 m of tank.
The tanks were then filled with sea water to a mean depth of 0.5 m
and flushed for an additional week.
The sea water for the system was pumped from the dock of the
Center for Energy and Environment Research at a depth of 3 meters.
The pump was a Sears Dynaglas 1 hp (230 volt). A plastic minnow
trap was used as a course prefilter for a Filterchem R combination
filter and foot valve at the pump inlet. All piping used was
grey schedule 40 P.V.C. The water traversed 73 m to a charcoal,
sand and gravel filter (0.5 x 0.5 x 1.0 m), then to a 1000 liter
settling tank. The settling tank was constructed of 1.9 cm
thick plywood lined with two coats of fibreglass R and epoxy resin.
The water was then gravity fed to the two sea water tanks with
the flow split using P.V.C. "T" joints. The flow rate,.in each
tank was monitored and maintained at 8.2 to 8.7 £ min~ by PVC
ballvalves. The total water volume was 3.1 x 10 liters. The
turnover time was 5.9 hrs per tank. The inflowing water was
diverted below the surface by submerging the inflow end using
a perforated inflow pipe (Fig. 2). The sea water was continuously
aerated with an air pump and air stones.
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Sewage Sludge
Activated sludge was shipped from New York City in 208 £ poly-
ethylene lined drums. The sludge was allowed to settle and the
overlying liquid decanted. In general, sludge is 5% solids. The
black, "oily" sludge slurry remaining was mixed in a large fiber-
glas tank. The sludge was added to a depth of 7.6 cm in an area
1 m wide by 2.3 m long (0.1 m3) at the incurrent end of the
Experimental tank which had previously been sealed off from the
rest of the tank. A PVC frame with small mesh screen attached
was placed over the sludge to prevent direct contamination of the
organisms. A duplicate screen was placed in the control tank.
After 24 hrs, when the sludge had settled, the seal was removed
and Experiment I was begun. At days 1, 5, 25, 50, 85 (Experiment II
only) and 125, after the sludge was added to the Experimental tank,
samples of organisms, sediment, sludge (day 1, 125 EXPI) and water
were taken from both tanks. A presample was taken prior to addition
of any sludge, in this study it is referred to as day 0.
Sampling Organisms
The initial stocking quantity or density for the biological
organisms, used in both Experiment I and II, is shown in Table 1.
TABLE 1. X'HYSICAL DI.MEHSIONS OF ORG/YNISMS WITH NUMBER Or TOTAL ORGANISMS
STOCKED PER TANK AND NUMV-BR OF ORGANISMS SAMPLED PER TANK PER WAY
EXPERIMENT I
Experimental Tank
H 8stocked #/Sample* L
Control Tank
H {{stocked &/Sample*
Clams
Sea Cucumbers
Snails
Oysters
Urchins
X 38
S.D 7
£i X
S.D
x "15
S.D 1
x 32
S.D 7
x
S.D
.3
.3
.3
.6
.6
.5
33.5
6.2
35.8
9.6
11.4
1.4
47.3
6.8
15
3
29
7
27
4
.1
.2
.2
.8
.7
.6
104
110
124
159
73.
10/
/20
q x'
/3S
10/
/ 1°
¥
/14
39.
7.
15-
1.
33.
8.
3
1
3
5
2
3
36.8
25.7
35.9
9.1
11.4
1.1
46.0
6.4
17.2
17.2
29.4
8.0
26.8
4.0
104
101
115
158
72
1C I/
/ 29
10 /
s~
%7
s/
/1 6
EXPKKIMiStJT II
Experimental Tank
Control Tank
Clams
Sea Cucumber:;
Snails
Oysters
Urchins
x
S.D
x
S.U
x
S.D_
y.
S.i>_
S.D
L
40
7.5
15.7
1.1
3D.O
9.2
W
34.6
6.3
44.6
17.8
11.6
0.7
5.2
*•
H S stocked t/i
15.8
3.4
35. 8
10.4
29.2
4.0
106
52
1BO
132
57
>C»Hl<-]t
'.0
5
20
10
5
•* L
39
7
15
1
38
9
.3
.5
,r>
. 1
.6
.5
W
36.1
7.4
44.5
13.4
11.5
0.0
47.4
5.7
H t
1h.4
3.2
35.2
10.4
29.7
3.5
Stocked */::.-i::.!.}c-*
106 10
52 5
180 20**
131 10
57 !>
•Upper valuos arc for days 0 through 50, Experiment J, lower values for
day 125. Numbers re Cur to number of organisms in each pooled sample.
**Snav]s net. ra'cr-n on day 125 control as none.- l.^ft.
5
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The organisms, except for the clams, were all collected
using manual techniques at Pta. Ostiones, Puerto Rico. The
organisms were allowed to equilibrate for 2 weeks in the flowing
sea water system. The turtle grass was replanted manually at
400 plants m , after soaking in a 10% NAPDH solution (Kelly
_e_t al. , 1971). The clams were collected at Pta. Viento on the
southeast coast of Puerto Rico.
Mangroves in Experiment I (230 seedlings) were planted as
ungerminated seedlings and as germinated seedlings in Experiment I
(120 seedlings).
Oysters in Experiment I, were placed in tanks and suspended
in one plastic mesh bag per tank. In Experiment II the oysters
were placed 13 per bag, for a total of 10 bags per tank. The
location of the bags was randomized except no bag was hung directly
over the sewage sludge deposit.
Table 2 is a species,
used for this study.
common name list of plants and animals
TABLE 2
Common name
Species
Clam
Oyster
Snail
Sea cucumber
Urchin
Turtle grass
Red mangrove
Codokia orbicularis
Crassostrea rhizophora
Nerita tessplata
Holothurea sp. (predominently
II. mexicana)
Lytechinus variegatus
Thalassia testudinum
Rhizophora mangle
After equilibration, organisms were sampled at the pre-
scribed intervals, the samples were removed, physical measure-
ments made, placed in double bags and frozen. Simultaneously,
samples were collected for hydrocarbon analyses. The samples
for hydrocarbon analyses were placed in glass bottles with
aluminum foil seals, capped and frozen. The bottles were previ-
ously rinsed with 2N HC&, deionized water, CCH , and air dried.
The physical dimensions for the animals sampled and number of
animals sampled per day are shown in Table 1.
Overlying Water
Water samples were taken, for Experiment I and II, prior
to biological samples. There were three types of water samples
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taken: pore water for analysis of soluble trace metals and hydro-
carbons, overlying water for soluble and particulate trace metals
and hydrocarbons, and dissolved reactive phosphate (DRP). The
DRP was sampled at weekly intervals. The trace metals and hydro-
carbons in water and pore water were sampled at the same inter-
vals as the organisms. Pore water samples were not taken in
Experiment II.
Pore Water
Samples were taken using one-inch PVC pipes sealed at one
end with 0.8 mm holes drilled in the lower 38 cm of the pipe.
The pipes were placed 46 cm from the sides at 1.2, 3.5, 5.8, and
8.1m from the intake end of the tanks. Two liter water samples
were withdrawn using 6.4 mm Tygon tubing and 25 Ibs of vacuum
pump.
Sediment
Sediment was collected using a 2.5 cm diameter x 25 cm
PVC core. The sediment core was homogenized and ground after
drying C1Q5°C) and a 1 g sample taken for trace metals. After
day 25 in Experiment I and throughout Experiment II, a 5 g sample
was taken for trace metals.
Dissolved and particulate trace metals
All water samples were placed in 2.5 I glass bottles
(which formerly contained cone. HNO or HCJ-) which were
equilibrated in reagent grade sea water for 12 hrs prior to
sampling (4 I samples for Experiment II). Th'e sample bottles
were drained, rinsed with the sample, filled, then filtered
within 4 hrs. The filtering was accomplished using an all
plastic filter system containing a . 4 ym pore size, 142 mm di-
ameter, Gelman filters. The filters were previously washed
in 6 N ECi and rinsed with copious amounts of R.O./D.I. water.
The fraction retained on the filter was referred to as the
"particulate" portion. The filtrate was the "soluble" fraction.
Physical and Chemical Analyses
General
All reagents used are reagent ACS grade. All glassware
was cleaned with detergent, rinsed with water from a Millipore
"Milli-Q" system. All water referred to in this paper is R.O./
D.I. water from the Millipore R "Milli-Q" system. This refers
to the technique of reverse osmosis followed by ion-exchange.
The final cleaning for glassware used to digest samples for
trace metal analysis involved refluxing the covered beakers
with concentrated HNO- followed by rinsing with R.O./D.I. water.
-------
All other equipment was washed with detergent, rinsed with
R.O./D.I. water, followed with 6N ECU and rinsed with R.O./D.I.
water. Only non-metallic instruments and materials were used
and all equipment, after cleaning, was stored covered with
Parafilm R or plastic bags.
Oxygen, temperature, and salinity
A Yellow Springs International YSI - 57 oxygen/temperature
meter was used. The oxygen output was both salinity and temper-
ature compensated. The oxygen calibration was checked using the
Winkler titration technique (Strickland and Parsons, 1965).
Dissolved reactive phosphate
One hundred m£ samples were collected and immediately
filtered through a Millipore Swin-Loc R adaptor containing an
acid washed (6N HCL) 27 mm Millipore, 0.45 m pore size filter
using an acid washed 100 m£ polypropylene syringe. The sample
was frozen and later DRP was determined using the single
solution technique (Strickland and Parsons, 1965) with a 10 cm
cell in a Beckman DU spectrophotometer. A small number of samples
were run on a Technicon autoanalyzer using standard Technicon
procedures CZimmermann, C. et al., 1977).
Particulate trace metals
p
The 142 mm diameter filters were placed in Pyrex beakers,
6 m£ of concentrated HNO : HC£ (3:1) were added and the beaker
covered with a ribbed watch glass. The solution was heated to
a slow boil (less than 95°C), when the filters dissolved, the
liquid was brought to a rapid boil and taken to near dryness.
Then, 4 m£ concentrated HNO were added, followed by dropwise
addition of 1 m£ of H^O,., C30% by volume). The sample was then
reboiled to near dryness and 3 drops of concentrated HUSOj. were
added. If the sample turned black, more H^O^ was addea. The
samples were dried until SO fumes disappeared. The sample was
then brought to 10 m£ volume with R.O./D.I. water.
Soluble trace metals
After 2 . 5£ of sample was filtered for determination of
"particulate" trace metals (41 in Experiment II), the effluent
was run through two 1.2 cm diameter x 10 cm glass columns. The
flow rate was maintained at 1 m£ min cm . The columns were
in series and the first column contained 10 m£ of Chelex-100 R
in the ammonium form (20 m£ in Experiment II) with 20 m& of
Amberlite XAD-2 resin (Rohm and Haas) in the second column.
The Chelex resin was purified by 2 bed volumes (BV) of 1NHC&
(isothermally distilled) followed by 5 BV of D.I./R.O. water
and then 2 BV IN NH OH (isothermally distilled) with a final
wash of D.I./R.O. water. The Amberlite XAD-2 resin was cleaned
-------
by washing for 10 minutes with D.I./R.O. water. The water was
decanted and the procedure repeated 5 times. The resin was
washed four times with anhydrous methanol followed by four
washes with D.I./R.O. water. The Chelex resin was left covered
with water prior to use. The Amberlite was stored under methanol
until just prior to use.
In Experiment I and II, the metals were extracted from the
Chelex-100 R resin after the resin column was first washed with
100 mi of water. The trace metals, in EXP 1, were eluted with
40 mi of boiling concentrated HC£. The resin was rinsed with
20 mi of water and this water was combined with the acid eluate.
The eluate was boiled down (95°C) to 1 ml and then brought to
10 mi volume with water. This was referred to as the soluble
inorganically complexed fraction. In Experiment II, the Chelex
100 was eluted batchwise using 6 mi concentrated HNO per 10 mi
resin (Davey, E.W. and A.E. Soper, 1975) rather than eluting
with concentrated HC£. The Chelex resin was filtered and the
acid eluate dried to 1 mi followed by the addition of 6 mi
2NHC£ (isothermally distilled) and dried down to 1 mi. This was
repeated and the final product was diluted to 10 m£ with distilled
water. The Amberlite resin was first washed with 100 mi water
then the resin was placed in an open glass Petri dish, rinsed
with methanol, and the resin ignited. The ignited resin was
placed in a muffle oven and the temperature raised in 100°C
increments until 400°C. The resin was maintained at 400°C for
1 additional hour then the temperature was raised to U50°C for
24 hours. The cooled resin was dissolved in 5 m£ 1NHC£, then
boiled down to 1 mi and brought to the desired final volume.
This fraction was referred to as the soluble, organically complexed
trace metal (Montgomery, J.R. and J.E. Echevarria, 1975). The
trace metals were determined by atomic absorption spectrophotometry
(A.A.S.) on a Perkin-Elmer Model 303 A.A.S with a deuterium back-
ground corrector. 'The output was recorded using a Model 56 Perkin-
Elmer recorder and DCR-1 digital readout. The flow chart for
sample collection and processing is shown in Fig. 3.
Biological and sediment samples
Analyses of National Bureau of Standards (NBS) bovine liver
and orchard leaves were performed along with the regular samples.
One gram samples were used, when available, for all standards
and samples (see Table 3). The flow chart for sample collection
is shown in Figure 3.
The number of organisms sampled per day per experiment is
shown in Table 1. The samples were defrosted after collection
and dissected into the following tissues using Pyrex glass shards
and plastic gloves.
Urchins - shell and internal tissues.
Sea cucumbers - body, muscle bands and internal tissues. In
Experiment II, muscle bands were combined with internal tissues
-------
Snails, clams, oysters - shell and internal organs,
Fouling organisms - total mass scraped from glass collecting
plates (23 x 30 cm). In Experiment I, all plates were sampled
at each sampling time. In Experiment II, cumulative results
were obtained by sampling plates which had remained in the
tanks for 25, 50, 85, 125 days after addition of sludge.
Thalassia - Roots and leaves. The roots and rhizomes were
combined and are referred to as roots.
Mangroves - The lower 3.8 cm of the hypocotyl not including
the roots. This is referred to in this paper as the bottom.
Growing stem includes all of the primary meristamatic tissue
from the hypocotyl excluding the leaves and the roots/rhizomes,
After dissection into these various parts, the wet weight
was obtained. The samples were dried in covered, cleaned Pyrex
beakers at 1Q5°C. The dried sample was weighed, then homogenized
using a ceramic mortar and pestle.
TABLE 3. RESULTS IN yg g" DRY WEIGHT FOR DETERMINATION OF METALS IN NBS
BOVINE LIVER AND ORCHARD LEAF STANDARDS. THE RESULTS FOR EXPERIMENT
I AND II ARE SHOWN WITH STATISTICS AND OUR COMPUTED MINIMUM DE-
TECTABLE CONCENTRATION (MDC).
MDC
X
SD
N
X
SD
N
NBS values
X
SD
N
X
SD
N
NBS values
Cd
0.3
0.56
0.26
9
Cd
0.55
0.45
49
0.27 +_
-------
The ground sample, not used for analysis, was stored in plastic,
screw cap bottles. The dried samples were placed in cleaned
Pyrex beakers, at least Ig where possible, and were digested using
the following procedure.
Twenty-five mi of a concentrated HNO : ECU mixture (3:1)
was added to the dried, weighed sample in the covered clean beaker.
The sample was slowly boiled and refluxed in the covered beaker
at 90 to 95°C until 1 mi of solution remained. The sample was
allowed to cool then 30 mi of 30% H 0» was added slowly and re-
heated to 90-95°C until 1 mi of the solution remained. A further
10 mi of HNO_:HC£ mixture was added and reheated. The mixture was
allowed to cool and 10 mi of 30% H?0 was added. This wa, allowed
to boil again (90-95°C). The 10 mi additions were repeated, if
necessary, a maximum of three times. Six m£ of 2N HC£ were added,
boiled at 90-95°C, until 3 mi were left. The sample was allowed
to cool then centrifuged in a clean 50 mi plastic capped centrifuged
tube at 1700 rpm for 20 minutes. The liquid was carefully decanted
and brought to 10.0 mi with R.O./D.I. water. This sample was
placed in clean, 25 mi polyethylene vials with caps.
11
-------
SECTION 3
RESULTS
The raw data, expressed as ym metal/g dry weight for all
samples, are found in Appendix A. The computed net uptake for
each metal is located in Appendix B and is expressed as mean
experimental results (yg/g dry weight) minus the mean control
results for each sample, with their respective standard devi-
ations, and degree of freedom. The "t" value for the null
hypothesis, Ho: M -M = 0 (P<0.05) where M is experimental and
M is control is also shown in Appendix B. The analyses for the
NBS biological standards.are shown in Table 3. The results for
all fractions of dissolved and particulate trace metals in the
water for both control and experimental tanks CExperiment II)
are in Appendix C. The wet/dry weight ratios with statistics
for all the samples in Experiment I and II are in Appendix D.
The experiments were replicated, Experiment I began on
11 March 1975 and ended 15 July 1975; Experiment II began on
December 12, 1975 and ended April 26, 1976. The experimental
tank refers to the tank with the sludge added and the control
tank was identical, in experimental design, to the experimental
tank except no sludge was added.
Temperature, salinity, and oxygen
In Experiment I the temperature ranged from 21 to 28°C
with a salinity range from 32 to 34°/0(?. In Experiment II the
temperature ranged from 21 to 26.5°C with a salinity range from
24 to 35°/00(x, 30°/00, S.D. 4.2°/00, N = 18).
Dissolved reactive phosphate (DRP)
The results are shown in Figs. u and 5 for control,
Experiment I and II and experimental, Experiment I and II,
respectively. The plotted points are the mean values for a
five day period.
Fouling organisms
In Experiment I, Cd, Cu, Pb, and Zn showed a significant
net uptake after 125 days (Figs. 6, 8, 9, 11), whereas in
Experiment II, Pb (Fig. 10) showed a steady uptake to the end
12
-------
of the sampling period (85 days). For Experiment II? Cd and Ni
demonstrated a steady uptake until day 50 (Figs. 7 and 13) then
a decline in the net uptake. Zinc and chromium indicated a net
uptake after day 50 (Figs. 12 and.15) and Cu showed little sig-
nificant uptake for the sampling period CAppendix B).
Sewage sludge
The initial values for trace metals in sewage sludge are
shown in Table H.
A rapid decline was noted in the concentration of Cd, Cr,
Cu, Ni, and Zn in the sewage sludge for Experiment I (Figs. 6, 15,
8, 14, 11). The Pb results are inconclusive for both experiments
(Fig. 9 and Appendix B) due to the loss of lead as the easily
volatilized PbSO during the digestion of the sludge. Because
of the shorter sampling interval for sludge in Experiment II, a
two stage decline for Cd, Ni, and Zn was shown (Figs. 7, 13, 12).
The first decrease in metal concentration was nearly instantaneous
with a further, smaller decrease after day 50.
Thalassia
Leaves
The results of EXP I were biased due to the "die off" of the
plants by day 50. This was probably due to insufficient light.
The plants survived for the full 125 dav period in Experiment II
after the light level was increased.
Copper, Cr, Pb, Ni and Zn showed rapid increases in net
uptake in Experiment I (Figs. 16, 18, 20, 22, 14). In Experiment
II the same overall trend of increasing net uptake was shown for
Cu, Cr, Pb, and Cd for Experiment II (Figs. 17, 19, 21, 26).
Nickel and zinc, in Experiment II did not show a significant
uptake up to day 50 but the net uptake was significant at day 85
and 125 (Figs. 23, 25).
Root
The only significant net uptake of trace metals for Thalassia
roots and rhizomes in Experiment I was for nickel, Cr, Cu and Pb
(Figs. 27, 29, 31, 33).
The repeat experiment also showed a significant net uptake
for Cr and Zn (Figs. 30, 36) with Pb, Ni, and Cu showing peaks
of significant net uptake values at day 5 and/or 25 days (Figs. 34
28, 32). Cadmium uptake was significant only at day 25 (Fig. 37).
Urchin, internal organs
To facilitate later discussion, the results of the net uptake
for urchins are shown with the results for Thalassia leaves.
13
-------
Copper, Cr, and Pb show a steady and rapid increase in net
uptake for EXP I and II (Figs. 16, 17, 18, 19, 20, 21). The net
uptake for Cd was not significant in Experiment I (Appendix B),
however in Experiment II (Fig. 26) the net uptake increased after
day 50. The results for nickel and Zn uptake are very similar
in Experiment I and II (Figs. 22, 23, 24, 25, 26). Zinc shows
a steady increase with a decrease, in net uptake, at day 50
followed by a rapid increase in both Experiment I and II.
Sea cucumber, internal organs
In both Experiment I and II, Cr, Cu, Pb and Zn showed very
similar net uptakes over the 125 day period (Figs. 38, 39, 40,
41, 42, 43). Ni showed a net uptake spike at day 5 in Experiment
I, and in Experiment II a spike showing uptake of nickel occurred
at day 5, 50 and a smaller spike at day 125 (Figs. 38, 41).
Mangrove roots
Nickel, in Experiment I, shows an initial decrease in net
uptake before day 50 with a gradual increase to day 125 (Fig. 27).
The opposite occurs in Experiment II where the net uptake increases
rapidly up to day 5 (Appendix B). The net uptake for Cr was not
significant for Experiment I (Appendix B), but indicated a linear
net uptake from day 50 to day 125 for Experiment II (Fig. 30).
Lead uptake in Experiment I and II (Figs. 33 and 34) exhibits
similar trends. However, Pb uptake is much higher in Experi-
ment I (Fig. 33). Zinc, in Experiment I, also demonstrated a
rapid linear uptake curve CFig. 35) which was very similar to the
uptake in Experiment II (Fig. 36). However, the net uptake was
greater for Zn in Experiment I. The net uptake for Cu was not
significant in Experiment I (Appendix B), but appeared to show
a gradual uptake after day 50. followed by a decline to origin-
al levels (Fig. 32).
Other
No significant net uptake was shown for the oysters, clams,
mangrove body, stem or leaves, snails, holothurian body and muscle
parts or sediment for Experiment I or II. The results shown are
in Appendix B. The significant results for net uptake of metals
are shown in Figs. 6-43 and are indicated by open rectangles.
Dark rectangles indicate non-significant results.
14
-------
TABLE 4. CONCENTRATION OF Cd, Cr, Cu, Ni, Pb AND Zn IN yg/g
DRY WEIGHT, IN SEWAGE SLUDGE USED IN THIS EXPERI-
MENT. THE RESULTS ARE FOR EXPERIMENT I AND II.
RESULTS FOR THE SAME METALS AS DETERMINED BY SALOTTO
AND FARRELL (1971). FOR ACTIVATED SLUDGE ARE INCLUDED.
EXPERIMENT I
Cd Cr Cu Pb* Ni Zn
X
SD
109
6.70
2120.
115.
2560.
124.0
60
55
319
11.3
2926
388.4
EXPERIMENT II
Cd Cr Cu Pb* Ni Zn
X
SD
166.
85.5
3667
338.5
2445.
266.4
21.0
3.29
376.
12.7
2508
122.1
SALOTTO AND FARRELL (1971)
Cd Cr Cu Pb* .Ni Zn
x 350 4330 1100 1500 380 3300
* Lead values are low due to digestion technique.
15
-------
SECTION U
DISCUSSION
Oysters and clams have been extensively studied regarding
trace metal uptake because of their commercial significance,
hardiness and feeding method. Bivalves, in general, show uptake
and concentration of most trace metals (Kerfoot, W.B., 1973;
Kerfoot, W.B. and S.A. Jacobs, 1973 a and b; Shuster, C.N. and
B.H. Pringle, 1969; Huggett, R.J. et al., 1973; and Frazier, J.N.,
1976). However, oysters and clams in our research showed no
consistent nor significant net uptake of metal except possibly
zinc and copper. In Experiment I, all the oysters were placed
in a single net bag. This procedure was changed to ten net bags
per tank in Experiment II. This drastically reduced the crowding
and increased the survival of the oysters beyond day 50 in
Experiment II. The wet weight of the clam and oyster internal
organs was too small to allow an adequate sample size for the
trace metal analyses. This same problem was encountered in the
analyses for the snails, holothurian muscle parts in Experiment
I, and zooplankton samples for both Experiment I and II.
The results for lead in the sewage sludge were very low
compared to other investigators CTable 4) and are due to the
addition of H SO to aid in the digestion of the sludge organic
matter. When the sample was heated, the lead was lost as volatile
PbSO . The H SO was only used for the digestion of the sludge.
The results for the analyses on the NBS bovine liver standards
(Table 3) are in good agreement for Cu and Zn and high for Cd.
Our results for Cr and Pb in bovine liver were below our de-
tection limits. The results for NBS orchard leaves were in good
agreement for Cu, Pb and Zn. Cadmium, in NBS orchard leaves,
was too high as was nickel. The coefficient of variation (CV)
for Ni was lower than the CV for Cd. The results for Cr, in
orchard leaves, were in good agreement with the non-certified
value for Cr published by NBS.
Our results for the concentration of trace metals in water
for Experiment I were at or below the detection limits for
nearly all metals and were therefore not shown. This was due
to the 2.5 I sample size. When we used 4 I sample in Experi-
ment II, the results for most of the metals were within our
detection limits. However, no significant difference was noted
between metal concentrations in the experimental tank and those
-------
In the control tank. Obviously, metals were released from the
sludge, as shown by the results in Figs. 6 through 15 and, also,
by the significant net uptake of metals by Thalassia leaves and
roots, urchin and sea cucumber internal parts, and fouling or-
ganisms. This net uptake In organisms was hard to explain when
no net change was seen in the water of the experimental tank.
The problem in interpretation of the water results was due to
the "point sampling" of the water versus the "integrated"
sampling for the organisms, sediment, and sludge. The "point"
concentration of the water was very low at any one time (Appendix
C). These trace concentrations, coupled with the high blanks
and matrix problems in trace metal analyses of sea water by
flame atomic absorption spectrophotometry, produced results with
very high within-sample variance. This high variance effectively
obscured any differences between the experimental and control
tanks in Experiment II. Schuster and Pringle (1969) demonstrated
that the concentration of metal in the water, at any one time,
may not be the best indicator of the total contamination by metals
The net uptake of metal by "fouling organisms" was clearly
shown in both Experiment I and II. This net uptake was replicated
in Experiment I and II for Cd, Pb, and Zn. The uptake of copper
by fouling organisms was only significant in Experiment I and
for Ni and Cr in Experiment II. The fact that Ni and Cr didn't
show a net uptake in Experiment I was probably due to a change
in sample technique for fouling organisms initiated in Experi-
ment II. In Experiment I, when a settling plate was sampled for
fouling organisms, clean plates were added for the next sampling
period, whereas, in Experiment II, a plate which was sampled
at day 125 had remained in the system from day 0 to day 125. The
fouling organisms sampled in Experiment II are, therefore, inte-
grated samples over the sampling time. The net uptake by the
fouling organisms of Cd, Pb, and Zn closely follows the loss of
these same metals from the sewage sludge for both Experiment I
and II. This same correlation, between fouling organisms and
sludge, holds true for Cd and NI uptake in Experiment II.
The net uptake by Thalassia leaves and urchin internal
organs closely parallel each other for Cu, Cr, Pb, Ni and Zn
in both Experiment I and II. Significant cadmium uptake by
Thalassia leaves and urchin internal organs was seen only in
Experiment II. Schroeder (1975), using radioactive Co, 60Co,
54Mn and 65Zn I.. :ea water, showed that, in Thalassia testudinum,
the rhizomes had the highest uptake followed by leaves, then roots
In both Experiments our results for Thalassia, show significant
net uptake for Cu, Cr, Ni and Pb in roots. The net uptake was
higher in Experiment.I for Pb and Cu and higher in Experiment II
for Ni and Cr. Cadmium and zinc had a net uptake In Thalassia
roots only in Experiment II. This uptake of metals by the
urchin and the close correlation between the urchin's uptake
and the uptake in Thalassia. leaves was not surprising as the
urchins were voracious foragers on Thalassia leaves and epiphytes.
17
-------
This was also shown in the field by Camp e_t al. , C1973), The
net uptake of some metals in leaves was closely coupled to the
uptake in the roots of Thalassia. The results for Cd, Ni, and
Cr in Experiment II indicate a translocation of these two metals
from roots to leaves. The translocation of metal was also
indicated for Zn in Experiment II from day 85, where the net
uptake decreases in the roots with an increase in the leaves
and vice versa at day 125. In general, the maximum net uptake
in Thalassia leaves was slightly greater than the uptake in
roots. This is in apparent contradiction of Schroeder's results
(1975) where roots were found to have the greatest uptake. How-
ever, our root sample consisted of roots plus rhizomes and, as
rhizomes showed the highest net uptake in Schroeder's paper
(1975), the contradiction between our results and Schroeder's
is cleared up.
The interpretation of the significant net uptake for mangrove
roots indicates that only Ni and Zn in Experiment I show a near
linear increase over time. In Experiment II, only Cr showed a
linear response over time, whereas, Zn and Cu showed an increase
in net uptake followed by attainment of apparent equilibrium,
then loss of the.metal. Lead shows no net uptake until day 85.
These results appear to be loosely correlated with the fact that
the sediment never showed a net uptake until at day 85 in
Experiment II when Ni, Pb and Zn began to show a slight net up-
take (Appendix B) in the sediment. Since no other part of the
mangroves sampled indicated a significant uptake of metal (Appendix
B), then the only source for the metals in the mangrove roots
must be the sediments. If that is so, then only Ni, Pb and Zn
should show net uptake in mangrove roots and then only after day
85 in Experiment II. This lack of significant uptake of metals
by the sediments could be due to the pooling of whole sediment
samples from a 2.5 cm diam. x 25 cm core. If the gradient of
trace metal concentration in the sediment was very steep, then
the pooling of the core could have diluted the higher surface
metal concentrations with the much lower metal concentrations in
the bottom of the core.
There were similarities in net uptake by holothurian guts
for Experiments I and II. Although there are similar patterns
of uptake in the holothurian gut, the maximum values for net
uptake were always higher in Experiment I for Ni, Cu, Pb and Zn.
Chromium showed a higher maximum net uptake in Experiment II
for holothurian guts. In Experiment II, the sewage sludge showed
a two stage release. The first stage occurred at day 1 followed
by a slow constant release until day 5-0 when another rapid re-
lease occurred. A slow but steady release would more likely
result in a higher maximum uptake, whereas, a two stage release,
as shown by the sewage sludge in Experiment II would, due to
the estimated 6 hour residence time for the water in the tanks
and 8.5 H min"1 flow rate, produce a lower maximum net uptake.
The data for the net loss of metals from the sewage sludge was
18
-------
only available for two sampling times in Experiment I, so it
is difficult to determine the true instantaneous net loss of
metals from the sludge.
The original objectives for this research, as stated in the
Introduction, have been obtained. A definite net uptake was
found in members of a tropical marine community- These results
were replicated at two different seasons and the results, con-
sidering the complexity of the system, agree quite well.
Originally, the fish, Diodon holacanthus (puffer) was to
be added to the system. However, after collecting over 360
specimens we found that they were too sensitive to environmental
factors other than sewage sludge and were easily killed. There-
fore, fish were deleted from the ecosystem in order to expedite
the completion of the experiment.
19
-------
SECTION 5
CONCLUSIONS AND RECOMMENDATIONS
Net uptake of trace metals by members of a Thalassia
testudinum/Rhizophora mangle community was demonstrated in two
125 day replicated experiments over the period from March 1975
to April 1976. The net uptake was especially significant in:
Thalassia testudinum leaves and roots/rhizomes, internal organs
of the urchin Lytechinus variegatus, "fouling organisms", internal
organs of Holothurea sp., and for some metals in Rhizophora mangle
roots. In general, the net uptake pathways followed the trophic
levels in the food webs. This pathway was especially noticeable
from the water to the fouling organisms and from the water to
the Thalassia testudinum leaves and thence to the urchin herbivore
Lytechinus variegatus. The net uptake in the Holothurea sp.
was closely related to the net uptake in the fouling organisms
and the trace metal loss rate of the sludge. The food web in
this artificial system was dominated by the fouling organisms as
would be expected. The uptake of trace metals, leached from the
sludge, was always greatest in this complex group.
The results of long term exposure, in a simulated tropical
ecosystem, to toxic trace metals more closely approximate the
situation in nature than do laboratory experiments. In this
respect we are in agreement with the statement by Phelps et al.,
(1975) "Chronic exposures to elevated metal levels through~~the~
food web should be used as a more realistic test for possible
deleterious effects from such metals as Cr". No artificial
system can ever reproduce a natural ecosystem. However, it is
more important to closely approximate the natural system with a
system which can duplicate itself (Menzel, D.W. 1977). Our
results show that chronic exposure to toxic metals can lead to
uptake and concentration of these metals in marine organisms.
We were able to duplicate our results using this complex simu-
lation of a marine ecosystem.
More detailed work on the net uptake of toxic metals, using
this complex simulated ecosystem, and the roles of the "fouling
organisms" and plankton in this system is needed. This must
involve integrated sampling of the organisms as well as the water.
The advent of more precise methods of direct trace metal determi-
nation in sea water using anodic stripping voltammetry and non-
flame atomic absorption spectrophotometry, in lieu of flame AAS,
should allow a reasonable sample size for both organisms and
water.
20
-------
The authors would not recommend, based on the results
of this research, the dumping of sewage sludge in shallow,
tropical marine environments. The potential exists for rapid
concentration of toxic trace metals by members of the food web
high on the trophic scale. These toxic levels could then affect
organisms used as food by man.
The next logical step in future research would be to add
vertebrate organisms to this system to see if a herbivore, which
normally consumes turtle grass, would show a net uptake of toxic
trace metals.
21
-------
30KM
{
*1 8°N 67°W
Figure 1. Study site location.
-------
PUMP
M-5.Oem ID PVC
f
•4- SAND FILTER
y HOLDING TANK
~(l.2xl.2x 1,2m)
•^-2.5cm PVC
SCREENED INTAKE
WITH FOOT VALVE
-e
CONTROL TANK
(0.9 XI. IX 9.0m)
EXPERIMENTAL TANK
(0.9 X I.I X 9.0m)
® INDICATES PVC BALL VALVES
CHARCOAL, SAND
AND GRAVEL FILTER
TOP VIEW
SEA WATER FROM
HOLDING TANK
=a CONTROL
(NO SLUDGE)
3 EXPERIMENTAL
(-1- SLUDGE)
OUTFLOW-* o
TO BAY
OUTFLOW _*o
TO FILTER
t
e
01
1
- 9.Om •
SIDE VIEW
SEA WATER — >•
SAND — >-
11 """ "
If It
t
1
:F
I
Figure 2.
Diagram of experimental design, tank configuration,
and sea water system.
22
-------
Addition of sewage
I
I
Collection of sediment/
sludge/organisms (0,1,
5,25,50,85**, 125 days)
sample taken*
Frozen in plastic bags for analysis
of hydrocarbons
Collection of water
samples for trace
metal and hydro-
carbon analysis
Filtered
Frozen
Subsample*
mercury
analysis
Defrost
Dissection
Sample pooled by tissue Extracted w~:th CCL. Chelex-100 resin
column
sample for* hydro- R
carbon analysis Amber!ite XAD-2
resin columns and
Extract stored in
freezer
wet weight
I
Dry 105°C
Elution, dilution
Analysis, atomic
absorption
Dry weight
I
Grind by
mortar/pestle
I
Digest/dilution
I
Analysis,
atomic absorption
Samples were collected at 0,1,5,25,50,85** and 125 days after addition of
sewage sludge.
*Samples for mercury, and hydrocarbons in both water, sediment, sludge and
organisms were stored frozen for future analyses.
**An additional sampling was made for Experiment II, Day 0 refers to the sam-
ple taken before addition of sewage sludge.
Figure 3 Flow chart of trace metal and hydrocarbon sampling
from collection processing into final sample form.
23
-------
O Nl T R CH L_ EIXF3 .
I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I
51 dE S r,
CM EM ru
ORVS
fcc • =
I
ID
i i i i i
m
ca
D>F1V!
Figure 4. Dissolved reactive phosphate concentration of
Experiment I and II, control. Data points are
mean values for 5 day period, excluding day 1.
24
-------
D>RF=>x EIXREIR I M ET M T R- L_ ET X F3 I
CE
I
in
ts
OFRV!
I I 1 I I
m
i i r i r
ETXREIR I M EZ N '
ETXF1
in
•H .
I .
I 1 1 I II I i T I i l T I I I I I I I I
_c m —
ORVS
Figure 5. Dissolved reactive phosphate concentration
Experiment I and II, experimental. Data points
are mean values for 5 day period, excluding day 1
25
-------
-------
CL
Q_
I
LJ
H
LU
OL
CL
cc
u
IMG CJRGRMISMS — ETX F>
9
-7
X OJ
En is
OFtVS
SETWRC3E:— ETXF3
I SB -I
rj
OR VS
Figure 7. Mean net uptake of Cd between experimental and
control, fouling organisms and sewage - Experiment II
27
-------
F-OLJL. i MC3 EDRGRM i SMS— E:XR . i
303 .
Q_
Q_
i
LJ
CC
LJ
ED
m
eg
ORVS
•cu
3K9B9B9 -,
2IZ1B9E) .
o_
Q.
z
CE
LJ
rsaca
S
ORV
Figure 8. Mean net uptake of Cu between experimental and control,
fouling organisms and sewage - Experiment I.
28
-------
RB F-nUI_ I ME CDRERN I SMS— E X R . I
0.
I
u
U
m
si
ORVi
— E:XR .
I 83 E
Q.
CL
a:
UJ
g
ORY
Figure 9. Mean net uptake of Pb between experimental and control,
fouling organisms and sewage - Experiment I.
29
-------
U>
o
RB F-OUL- I ME ORC3RN I SMS— ETXR . 2
sea .
CL se>
CL
I
LJ
£ IB
ca
1 1
ru
ta
D-RVS
Figxire 10. Mean net uptake of Pb between experimental and control, fouling organisms - Experiment II
-------
ZM F-C3UI_ I ME OF3BF1M | SMS— ETXF= . I
I EB3 .
21 1Hla :
CL
Q_ I •ZB .
I
UJ
CE
U
i taa .
BB
SB
MB
2B
ORVS
iEZUJREET— EIXR . I
2 El El El .
I SBB .
Figure 11. Mean net uptake of Zn between experimental and control,
fouling organisms and sewage - Experiment I.
31
-------
:M f=-aui_ i ME DRCHRN
SMS— EXR
0_
N
^>
Ld
cr
LJ
-i 1
g
ORYS
— EIXR . 2
3ZJEDD _
a.
a.
cc
LJ
—J_ I
ORV!
Figure 12. Mean net uptake of Zn between experimental and control,
fouling organisms and sewage - Experiment II.
32
-------
M I F-ai_JL_ I MB CJRGRM 1 SMS— ETX(=
SB .
a.
D_
s
V
u
D»f=IVS
M t
— ETXR
z:
CL
Q-
•z.
cn
Ul
3SS3 .
30303 .
2SB3 .
2 £003 .
1 S03 .
1 I
*
, |
i
ORVS
Figure 13.
Mean net uptake of Ni between experimental and control,
fouling organisms and sewage - Experiment II.
33
-------
•CR SETWRGE: — EXR . i
ZS29S9 _
Q_ I 5090
CL
z
CE i tataia
g
i i i
onvs
M i SETWREE:— E:XR .
0.
OL
UJ
zsei .
2tnizj .
t del .
SB9
BI
ORYS
Figure 14. Mean net uptake of Cr and Ni between experimental and
control, sewage - Experiment I.
34
-------
F- C3 LJ l_ I M C3 C3RCSRMISMS — ETXR
Q_
D_
Ul
ir
LU
2:
ORVS
SETUJREE:— EZXR .
3s:lain .
Q.
d.
CC
Ul
z:
i ssaes .
SE3B3
ORVS
Figure 15. Mean net uptake of Cr between experimental and control,
fouling organisms and sewage - Experiment II.
35
-------
•CU T|
IRL.RSS I R L_ETRVErS — ETXF=
Q_
Q.
I
LJ
v^
cr
u
2:
Q.
0_
I
U
CE
U
•HZI .
I 0 .
-i r
m
si
£
-------
•CLJ TWRLRSS 1 R LETRVETS— ETXR
a.
a.
N
LJ
CL
LJ
£ i
ORVS
UR
-------
-------
-------
RB TMRLRSSIR LETRVETS— ETXR. I
I
Ul
DC
UJ
MB3 .
CD
SI
s
[>RVS
RB UF?
-------
RS -THRLRSS I R LETRVETS— ETXR
Q.
O_
LJ
cn
u
ORVS
UR
-------
M I TMRL-RSS I R L-ETRVETS— ETXR . I
CL
D_
LJ
GC
U
CL
0_
I
LJ
CE
U
21
ZCB _
a x m —
^P ^g f^l
ORVS
M I LJR-CM I M BLJ~T— EIXF3 .
2B3 -
I B3 .
1—
Kl
m
ta
is
ORV!
Figxire 22. Mean net uptake of Ni between experimental and control,
Thalassia leaves and urchin guts - Experiment I.
42
-------
M I TMRURSS I R LETRVes— ETXR . S
O.
O.
•set .
I BJ .
£ &
ORVS
Nl I
I Nl C3UT— (CXF1
0.
O.
•s
U
CE
U
i
ORVS
Figure 23. Mean net uptake of Ni between experimental and control,
Thalassia leaves and urchin guts - Experiment II.
43
-------
ZM TMRI—RSS I R l—ETRVETS— ETXF3 . I
0.
D-
I
U
£ 8
ORVS
ZM UR
-------
ZNI THRLRSS I R L-ETRVETS — ETXR . 2
0.
CL
UJ
2
CC
Ld
i
ORV!
ZM URCMIM BUT— EXR
MBS .
CL
CL
UJ
CD .
2 IB .
|
ORY!
Figure 25. Mean net uptake of Zn between experimental and control,
Thalassia leaves and urchin guts - Experiment II.
45
-------
-------
NJ I
ETXR
fc s
I
U
cr -*
UJ
Of=IVS
M I
I (=1 ROOTS— EIXF1 I
z:
CL
2C9 .
EL
LU
_l ( r , ,—
01 £ i
ORVS
Figure 27. Mean net uptake of Ni between experimental and control,
Thalassia roots and mangrove roots - Experiment I.
47
-------
M
VE: .ROOT — ETXF> .
g.
I
u
s^
CE
LJ
ru
is
ORY!
M i
THRLRSS I R ROOTS— ETXR
BZJ -,
CL
n.
Lul 2E1 .
cc
LJ
1—
IS
m
is
Figure 28. Mean net uptake of Ni between experimental and control,
Thalassia roots and mangrove roots - Experiment II.
48
-------
-------
-------
-------
-------
RB TMFtt_RSS I R ROOTS— ETXF9
U)
r:
Q.
0-
I
U
CE
LJ
z:
i
ORVS
Figure 33. l^tean net uptake of Pb between experimental and control, Thalassia roots - Erperirent I
-------
RH MRNGRCIVE: ROCHT— ETXR
I S -
LJ
S
ORY
THRLRSS I R RdCITS— ETXR
0.
0.
LJ
CE
LJ
— 11
ORVS
Figure 34. Mean net uptake of Pb between experimental and control,
Thalassia roots and mangrove roots - Experiment II.
54
-------
ZM MRMERCDVEr ROOT— ETXF3
en
cn
CL
Q.
I
LJ
DC
U
x
SI
OR Vi
Figure 35. Mean net uptake of Zn between experimental and control, mangrove roots - Experiment I,
-------
MRNGRnVET
— ETXF"
D_
n_
i
LJ
IT
Ld
B B M
eg
onvs
ZM TMRI— RS5 I R ROOTS— EIXF3
n_ i ei -i
D_
I
Ld
CC 63 -
Ld
ORVS
Figure 36. Mean net uptake of Zn between experimental and control,
Thalassia roots and mangrove roots - Experiment II.
56
-------
•CO THRL-RSS I R ROOTS'
E:XR
i -
Ul
CL
CL
I
LJ
CC
LJ
z:
m
is
1—
El
C>RVS
Figure 37. Mean net uptake of Cd between experimental and control, Thalassia roots - Experiment II,
-------
•CR Hai—OTMUR I RM GUT — ETXR . I
0.
a.
LJ
en
u
ORVS
M I Hai_tUTMUR I RM GUT — ETXR . I
Q_
a. M
u
ffi
ORYS
Figure 38. Mean net uptake of Cr and Ni between experimental and
control, sea cucumber guts - Experiment I.
58
-------
Q.
I
U
cc
u
ID .
I RN GUT — ETXF>
-i r
g
OR VS
MdL-DTMUR I
C3UT— ETXF3
sria
tL
n_
LJ
i (a .
— I 63
onvs
Figure 39 . Mean net uptake of Cd and Cr between experimental and
control, sea cucumber guts - Experiment II.
59
-------
<:U HDt_C3TMLJRIRN
— ETXR.
CL
CL
I
U
cc
LJ
2E3 _
i ei .
I
S3
X
IS
CD
SI
hJ
ORVS
Figure 40. Mean net uptake of Cu between experimental and control, sea cucumber guts - Experiment I.
-------
u
z:
I RM
Tr- EXR
O.
Q.
V.
SB3.J
383 J
CL
Ul
M
El
ORVS
Figure 41. Mean net uptake of Cu and Ni between experimental and.
control,- sea cucumber guts - Experiment II .
61
-------
RB M Ol_ CD ~T M LJ R I RN GUT — ETXR . |
CL
D_
LJ
CE
LJ
GB3 .
ME) .
-\ - 1
1
x
ea
m
ta
D>RY!
MdL-OTMUR I RN BUT— ET X R . I
srei _
0_
(X i ei _
Figure 42. Mean net uptake of Pb and Zn between experimental and
control, sea cucumber guts - Experiment I.
62
-------
MOL-DTMUR I Fl M GUT — ETXF3 .
CL
D_
I
Ld
cn
LJ
I S3 -I
.,_ ,
ORYS
F=1M
0_
OL
I
UJ
CT
U
is J
s 4
is
£ g
onvs
Figure 43.
Mean net uptake of Pb and Zn between experimental and
control, sea cucumber guts - Experiment II.
63
-------
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67
-------
APPENDIX A
Sorted Raw Data Experiment I and II
There are eleven (11) columns shown which represent the
following:
Column
1 - Animal type
C = Clam
H = Holothurian
M = Mangrove
T = Thalassia
P = Fouling Organisms
U = Urchin
S = Sediment
2 - Tissue type
M = Muscle
I = Internal contents (guts)
S = Shell
B = Body/Bottom
L = Leaves
R = Roots and rhizomes
G = Upper Meristem
D = Solids
3 - Experiment number - 1(1) or 11(2)
4 - Sample type - whether from experimental tank (0) or
control tank (1)
5 - Represents sample time
0 = presample
1=1 day after sewage added
5=5 days " " "
25 = 25 " " " "
50 = 50 " " " "
85 = 85 " " " "
125 = 125" " " "
68
-------
6 = Concentration ( vg/g dry weight) Cd
7 = " " Cr
8 = " " Cu
9 = " " Ni
10 = " " Pb
11 = " " Zn
Multiple values for the same sample represent replicate
samples. The value -999.9 is an indication of a value that could
not be detected. All manipulation of the data used one half
minimum detectable concentration CMDC) when a value was less
than or equal to the MDC. The MDC values are listed in the
Results CTable 4).
69
-------
SORTED DATA
c
c
c
c
c
c
c
c
c
c
c
c
^
c
c
c
c
r
r
c
c
c
c
c
c
c
c
c
c
c
c
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
I 1
[ 1
C I 1
C I 1
C I 1
C 5 1
C S 1
C S 1
C 5 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
C S 1
0
0
1
1
1
1
0
0
0
1
1
0
0
1
1
0
0
1
1
0
0
0
1
1
1
0
0
0
0
0
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
0
0
0
0
1
1
1
1
1
5
5
5
S
25
25
25
25
50
50
50
50
50
50
125
125
125
125
125
125
125
125
125
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
5
5
2.4
2.7
-999.9
2.0
.9
1.0
-999.9
-999.9
.9
-999.9
A. 2
-999.9
-999.9
3.7
-999.9
2.0
2.0
-999.9
2.0
2.4
3.5
1.2
5.0
3.6
1.5
1.6
1.6
1.3
1.4
1.4
2.0
2.0
1.6
1.5
4.0
4.0
3.3
3.1
4.0
3.0
3.4
3.4
-999.9
4.6
3.1
i.i
-999.9
-999.9
3.1
3.1
-999.9
-999.9
-999
-999
-999
1
1
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
3
5
-999
3
3
1
1
2
-999
-999
-999
-999
2
2
3
-999
-999
6
-999
-999
1
3
-999
5
6
6
-999
-999
6
6
-999
..9
.9
.9
.0
.7
.1
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.8
.7
.8
.9
.5
.0
.9
.4
.1
.9
.9
.9
.9
.8
.5
.1
.9
.9
.7
.0
.9
.9
.4
.1
.9
.2
.4
.«
.9
.9
.9
.9
.9
-999^9
31.6
31.4
17.5
16.4
14.4
17.4
10.6
10.0
7.0
21.9
26.2
11.2
9.9
20.6
19.6
9.8
12.3
27.4
15.2
9.7
6.5
9.5
10.6
7.5
5.8
5.5
4.5
4.7
6.0
6.4
1.8
5.2
5.1
5.3
1.2
1-4
6.1
.0
1.4
1.4
5.6
5.4
1*4
.0
6.0
6.1
1.3
1.2
6.2
6.2
2.4
1.6
-999
-999
-999
6
6
7
-999
-999
7
-999
-999
-999
-999
-999
-999
-999
9
-999
10
9
5
7
14
11
9
5
2
2
6
6
2
5
7
7
-999
-999
32
33
-999
-999
33
34
-999
36
34
34
-999
-999
.9
.9
.9
.7
.9
.9
.9
.9
.5
.9
.9
.9
.9
.9
.9
.9
.1
.9
.4
.4
.0
.6
.1
.3
.6
.0
.0
.8
.8
.6
.0
.0
.9
.6
.9
.9
.9
.8
.9
.9
.7
.0
.9
.9
.0
.0
.9
.9
33.7
34
-999
-999
.0
.9
.9
-999.9
-999.9
-999.9
4.5
5.0
5.9
-999.9
-999.9
6.5
-999.9
-999.9
10.2
14.1
-999.9
-999.9
-999.9
-999.9
-999.9
6.6
5.6
10.5
10.0
12.7
10.7
6.1
5.8
4.4
5.3
5.9
5.8
6.2
6.2
4.0
4.7
20.8
20.0
49.8
.0
10.5
13.4
49.6
54.5
13.5
13.3
57.0
S3»9
11.4
15.9
5-1.0
51.9
20.0
11.5
81.0
83.0
108.2
167.6
89.4
107.4
164.7
164.3
191.2
270.1
306.9
216.3
241.9
211.4
216.0
187.2
184.6
85.2
101. 6
187.1
194.0
168.3
353.6
268.3
187.5
139.6
109.6
99.7
119.0
114.6
69.8
119.5
120.5
117.1
1.7
2.6
3.0
3.6
3.3
1.5
4.6
5.2
1.6
5.5
3.3
3.6
.5
.3
4.2
.0
1.3
.9
70
-------
SORTED DATA
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
c s
€ S
c s
c s
c s
c s
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
h B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
M B
H B
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
1
1
0
0
0
0
1
1
0
0
1
1
0
0
1
1
0
0
0
0
1
1
0
0
1
1
0
0
0
0
1
1
0
0
5
5
5
5
5
5
25
25
25
25
25
25
25
25
50
50
50
50
50
50
125
125
125
125
125
125
0
0
0
0
1
1
1
1
5
5
5
5
5
5
25
25
2f
25
50
50
50
50
50
50
125
125
3.6
3. A
-999.9
-999.9
3.3
3.5
-999.9
A. 6
3. A
3.3
-999.9
-999.9
3.5
3.7
5.0
5.0
3.7
3.5
A. 6
A. A
5. A
5. A
3.3
3.3
A. 9
5.3
l.A
1.5
2.3
2.5
-999.9
1.5
-999.9
1.6
l.A
1.3
2. A
2.5
l.A
1.3
1.3
1.6
1.0
.8
2.1
2.7
2. A
2. A
9.8
2.0
.9
.9
3
2
-999
-999
2
2
-999
3
1
2
-999
-999
-999
2
3
3
2
3
3
3
3
3
2
2
A
3
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
1
2
2
2
1
1
1
.1
.9
.9
.9
.7
.3
.9
.8
.3
.5
.9
.9
.9
.5
.8
.A
.0
.1
.5
.A
.2
.7
.7
.6
.7
.7
.9
.9
.8
.3
.9
.9
.9
.A
.9
.9
.5
.5
.9
.9
.9
.6
.9
.9
.3
.3
.0
.6
.0
.5
.7
.A
5.5
5.6
1.6
1.7
5.5
5.6
1.2
6.3
5.8
6.2
l.A
1.2
5.4
5.2
6.5
6.7
5.2
5.1
6.9
6.5
5.8
5. A
6.C
5.5
5.5
5.5
1.8
2.0
2.8
3.3
1.7
3. A
1.9
1.8
1.6
2.1
2. A
2. A
1.5
1.2
2.0
2.0
1.5
1.2
2.1
2.7
3.0
3. A
9.8
2.0
1.7
2.1
33
32
-999
-999
33
33
-999
3A
33
-999
-999
33
33
31
30
33
3A
36
36
32
.2
.9
.9
.9
.7
.8
.9
.0
.6
*7
.9
.9
.a
.9
.1
.8
.7
.2
.6
.8
.8
33.0
33
33
3-0
31
13
10
11
12
8
11
13
11
10
10
10
11
10
11
12
6
6
10
10
7
11
9
.8
.2
.0
.9
.9
.0
.9
.0
.9
.9
.0
.0
.9
.0
.0
.A
.0
.0
.0
.9
.9
.2
.0
.0
.0
.7
.0
.0
.0
.0
A2.1
A1.2
11. A
11.7
A6.8
50.7
17.2
1A.O
A9.0
A 9. 7
10.1
9.3
A3. 8
A2.9
1A.3
8.8
A2.7
A2.0
15,0
12.7
A9.3
A5.6
A9.6
A3. 9
A3. 3
A6.6
6.1
7.9
10.5
10.6
2.8
11.3
3. A
7.6
11. A
12.0
11.9
12.6
6.7
6.2
2.9
12.3
6.1
5.5
2.8
2.8
10.8
1A.7
2.9
3.2
10.5
12.0
A. 3
3.6
.2
J.5
3.9
3.9
.6
6. A
A. 2
A. 3
-999.9
.2
3.7
3.6
6. A
A. 7
20.8
26.6
7.9
5.0
A. 3
2.5
7.0
3.6
3.0
3.2
19.8
16.3
20.3
25.1
9. A
11.9
8.0
10.0
9.9
1A.O
12.5
1A.1
8.7
7.5
10.0
9.9
7.7
5.6
29.9
9.0
10.8
11.8
15.1
12.0
6.5
7.5
71
-------
SORTED DATA
H E
H t
H I
H 1
H 1
H
H
H
H
M
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H
H 1
H 1
H 1
H 1
H 1
H 1
H 1
H 1
H 1
H
H |
H
H
H
1 1
1 1
1 1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
I 1
1 1
! 1
1
1
1
1
1
1
1
1
1
1
1
1
1
* I
1 1
1 1
1 1
1 1
* 1
« 1
1 1
I 1
ft 1
K 1
ft 1
1 1
ft 1
1
1
1
0
0
1
1
1
1
1
0
0
0
1
1
0
0
1
1
0
0
0
1
1
1
1
0
0
1
1
1
1
0
0
1
1
1
1
0
0
0
0
0
1
1
1
1
0
0
c
0
1
125
125
125
0
0
0
0
0
0
0
1
1
1
1
I
5
5
5
5
25
25
25
25
25
25
25
50
50
50
50
50
50
125
125
125
125
125
125
1
1
1
1
1
1
1
1
1
5
5
5
5
5
1
2
2
1
1
1
1
2
2
1
2
2
-999
1
2
-999
-999
1
3
3
3
2
.999
3
Z
a
3
1
1
2
2
2
2
2
2
3
3
-999
1
1
2
1
-999
1
2
2
-999
2
2
-999
.4
.5
.4
.0
.3
.1
.1
.0
.3
.3
.5
.4
.4
.9
.7
.0
.9
.9
.5
.3
.8
.6
.1
.9
.4
.8
.7
.7
.6
.6
.6
.7
.3
.4
.1
.1
.4
.4
.9
.4
.4
.0
.0
.9
.6
.0
.0
.0
.9
.8
.4
.9
-999
1
1
8
7
13
22
18
19
19
7
11
10
9
10
14
12
7
5
27
25
12
10
11
12
27
31
8
10
10
29
29
18
18
17
17
-999
2
2
2
-999
3
3
3
3
3
3
-999
.9
.7
.4
.0
.0
.3
.3
.6
.2
.2
.0
.8
.6
.0
.8
.9
.9
.0
.3
.0
.0
.0
.0
.4
.5
.6
.9
.9
.0
.0
.5
.6
.6
.7
.0
.0
.6
.6
.9
.0
.3
.3
.9
.8
.2
.7
.6
.4
.2
.6
.1
.9
1.6
3.0
2.6
8.6
10.1
9.9
14. &
11.9
8.5
8.0
10.1
10.1
9.9
8.7
11.1
15.0
15.0
8.4
8.6
.0
31.4
28.2
10.1
.0
9.5
10.1
33.9
40.8
.0
.0
9.5
10.4
25.7
27.9
13.0
20.0
12.9
13.5
2.7
3.2
2.7
4.7
3.5
2.4
3.2
5.8
4.8
5.6
4.0
5.5
4.9
2.6
.0
7.5
7.8
17.1
19.9
20.1
30.9
25.8
22.6
21.8
13.0
15.8
14.8
19.-0
23.7
24.9
24.8
16.9
15.8
.0
22.4
21.4
28.0
17.4
21.*
18.9
21.0
23.9
.0
19o9
21,7
21.9
23.7
24.0
28.0
28.9
28.8
27.9
7.9
8.6
.0
8.9
7.0
8.0
11.6
10.8
10.8
11.9
8.0
10.8
10.8
8.0
9.0
12.6
12.8
5.9
2.4
5.9
12.0
11.9
11.7
11.3
.0
16.7
16.8
2.8
4.0
17-.3
25.8
2.8
3.3
.0
66.2
60.2
.0
13.4
14.8
11.9
75.8
82.8
12.0
10.6
15.8
15.9
71.0
65.3
.0
25.0
22.9
19.9
1.6
10.5
11.9
9.9
9.8
1.6
12.0
9.8
9.8
3.0
1*6
12.8
12.8
9.f
6.1
11.8
10.8
37.9
2,6.3
33.4
42.7
41.0
43.9
45.7
69.8
56.4
55.9
31.8
37.5
56.7
55.6
29.0
27.3
.0
68.6
62.0
37.0
.0
34.6
36.0
79.8
79.7
27.9
19.9
35.2
35.6
60.8
64.4
46.9
43.9
41.4
43.9
54.3
99.5
.0
66.8
64.2
40.5
65.8
57.6
57.8
69.1
47. Q
70.2
63.8
43.6
72
-------
SORTED DATA
H H
H N
H M
H M
H M
H H
H M
H M
H M
H M
H M
H M
H M
H N
H H
M 6
M B
H B
H B
H B
H B
H B
H B
H B
M B
H B
N B
H B
M B
H B
H B
H G
M G
H G
H G
M G
N G
H G
M G
H G
M G
H G
H G
M G
M G
M G
H L
H L
N L
H L
H L
N L
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
1
1
0
0
0
0
0
0
1
1
1
1
0
0
0
1
1
1
0
0
1
1
0
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
1
0
0
1
1
0
0
1
0
0
1
5
25
25
25
25
50
50
50
50
125
125
125
125
125
125
0
0
0
0
0
0
5
5
5
5
50
50
50
50
125
125
0
0
0
0
0
5
5
50
50
50
50
125
125
125
125
5
5
5
50
50
50
1.4
-999.9
-999.9
1.7
-999.9
1.0
1.0
2.2
2.4
1.2
1.4
1.1
1.7
3.6
3.5
.5
.0
.5
.3
.5
.5
1.5
.5
.4
.4
.7
.6
.7
.7
.7
1.0
.3
.3
.3
.3
.4
1.2
1.0
.6
.8
1.4
1.4
1.0
1.0
1.1
1.0
.6
.8
1.5
1.1
1.0
1.1
1
-999
-999
-999
-999
3
9
10
4
3
3
5
6
6
3
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
3
*
-999
-9*9
4
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
.6
.9
.9
.9
.9
.9
.4
.2
.6
.0
.4
.2
.4
.8
.8
.9
.1
.1
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
•9
.9
.9
.7
.1
.1
.9
.9
.8
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
3.6
4.5
4.7
3.4
2.7
3.1
2.2
6.4
7.6
4.7
4.5
2.9
6.2
7.0
7.0
3.2
2.5
3.1
2.9
2.9
2.9
2.1
2.0
2.6
2.5
2.6
2.1
2.7
2.5
2.7
3.6
3.9
3.8
3.1
3.1
2.9
4.0
a. 3
4.4
4.9
6.0
5.9
4.4
4.2
4.2
4.2
4.3
4.2
9.0
7.0
6.3
7.4
9.1
3.9
3.8
3.9
3.9
11.8
7.9
13.2
16.0
9.9
10.0
15.9
10.0
19.9
19.6
1.9
1.7
1.6
1.5
1.4
1.2
3.0
3.0
.0
.0
.0
.0
3.0
3.0
.0
.0
2.2
1.8
1 .9
2.3
2.4
2.4
9.6
2.2
2.3
1.6
1.8
*.o
3.4
2.1
2.6
.0
.0
4.5
3.4
3.5
7.0
9
11
14
13
13
12
7
12
13
2
2
5
8
16
15
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
3
-999
-999
3
4
-999
-999
-999
-999
-999
-999
16
4
4
-999
-999
5
3
4
3
-999
-999
-999
5
4
7
.5
.6
.2
.9
.2
.2
.1
.0
.3
.9
.9
.3
.8
.9
.7
.9
.0
.S
.9
.9
.9
.9
.9
.9
.9
.9
.0
.9
.9
.0
.0
.9
.9
.9
.9
.9
.9
.0
.0
.0
.9
.9
.0
.0
.0
.0
.9
.9
.9
.0
.0
.0
47.5
67.9
67.5
62.1
66.6
39.2
29.5
46.1
52.7
51.7
47.8
32.9
42.8
56.5
51.6
4.5
5.1
4.S
4.3
5.6
4.3
1.4
2.5
1.9
1.8
2.6
2.2
1.6
2.1
2.S
3.)
6.2
5.7
5.7
5.2
6.0
3.8
31.6
2.8
3.1
4.8
6.7
6.<»
4.9
4.3
VI
8.3
a.o
11.5
8.1
7.5
11.5
73
-------
SORTED DATA
H L
M L
M L
M 4.
M L
M R
M R
M R
H R
* R
H R
M R
* R
M R
H R
H *
H R
M R
H I
N I
N I
K I
N I
N I
N I
N I
N I
N I
N 5
N 5
N S
N S
N S
N S
N 5
N 5
N S
M S
N S
N S
N 5
N 5
N 5
N S
N 5
N S
N S
N 5
N S
N 5
N S
M S
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
1
1
0
0
1
1
0
0
1
1
0
e
i
i
i
0
1
1
0
1
0
1
0
1
0
0
0
0
0
1
1
0
0
1
1
0
0
1
1
c
0
1
1
0
0
1
1
1
1
50
125
125
125
125
5
5
5
5
50
50
50
50
125
125
125
125
125
0
0
1
5
5
25
25
50
SO
125
0
0
0
0
0
, 0
1
1
1
1
5
5
5
5
25
25
25
25
50
50
50
50
50
50
1.2
1.0
.9
1.2
1.1
1.2
1.2
1.1
1.0
1.8
1.8
1.6
1.4
1.6
.5
1.2
1.2
1.1
5.4
3.5
-999.9
3.1
-999.9
-999.9
-999-9
4.1
3.2
3.0
-999.9
-999.9
5.7
5.5
-999.9
-999.9
-999.9
-999.9
-999.9
-999.9
-999.9
-999.9
-999.9
3.5
-999-9
-999.9
-999.9
-999 .9
4.2
4.3
4.2
3.6
5.1
5.0
-999
-999
-999
-999
-999
-999
-999
1
1
1
e
6
3
4
2
10
3
-999
-999
-999
-999
7
5
-999
41
-999
-999
6
6
-999
-999
-999
-999
-999
-999
-999
4
-999
-999
-999
-999
-999
-99^
3
3
2
2
6
6
.9
.2
.9
.9
.9
.6
.9
.9
.9
.0
.0
.0
.0
.0
.0
.8
.2
.9
.8
.5
.9
.9
.9
.9
.4
.6
.9
.6
.9
.9
.8
.5
.9
.9
.f
.9
.9
.9
.9
.7
.9
.9
.9
.9
.9
.9
.4
.8
.1
.3
.9
.$
7.7
5.4
6.0
6.1
5.9
4.0
3.3
3.7
3.*
4.6
4.5
4.4
4.0
8.6
6.3
4.8
4.0
3.5
46.9
55.2
62.2
58.4
50.3
56.0
49.6
53.8
50.7
21.4
-999.9
-999.9
6.3
6.3
2.0
3.0
-999.9
-999.9
3.0
3.0
-999.9
-999.9
3.0
2.0
-999.9
-999.9
7.6
3.9
6.6
6.8
6.6
6.0
6.2
6.1
8
6
6
9
9
5
17
15
10
10
10
27
27
-999
24
-999
-999
-999
16
17
9
-999
-999
33
35
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
32
33
33
32
32
33
.0
.0
.0
.0
.0
.0
.0
.0
.0
.0
.0
.0
.5
.9
.0
.8
.8
.5
.0
.4
.9
.0
.9
.9
.9
.4
.3
.9
.9
.9
.8
.6
.9
• 9
.9
.9
.9
• 9
.9
.0
.9
.9
.9
.9
.9
.9
.7
.6
.6
.6
.6
.0
6.0
6.0
5.0
4.0
4.0
4.0
4.0
5.0
6.0
8.0
7.0
6.C
5.0
11.0
10.0
8.9
6.9
6.7
40.9
20.8
23.3
ia.i
28.7
34.2
34.4
6.2
16.7
3.8
79.1
79.3
46. 6
48.7
75.1
76.6
74.8
72.5
61.8
56.8
81.7
5-1,8
72.6
73.1
72.6
72.8
71.9
67.6
42.6
43.8
41.8
38.7
47.0
44.4
12.0
10.0
10.5
7.*
7.«
12.5
10.6
8.1
7.6
24.0
23.9
12.3
10.3
53.8
44.9
26.0
33.5
31. 4
119.7
129.3
173.6
157.0
168.6
116.5
189.1
168.5
141.8
82.3
8.9
5.2
5.6
6.5
7.4
7.5
8.6
9.6
12.3
11.6
8.9
7.0
9-4
8.6
11.5
9.7
11.4
13.6
7.1
7.1
7.9
8.6
7.0
6.4
74
-------
SORTED DATA
N
N
0
Q
0
0
Q
0
0
0
0
0
Q
0
Q
0
0
0
Q
0
0
0
0
0
0
0
0
0
0
0
0
0
P
P
P
P
P
P
P
P
P
S
S
I
S
S
5
S
5
S
5
S
5
S
5
5
S
S
5
S
5
S
5
S
D
D
D
D
D
D
D
D
D
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
0
0
1
1
0
1
0
1
0
1
0
0
1
1
0
0
1
1
0
0
0
0
1
1
0
0
0
0
1
1
0
0
1
1
0
1
0
1
1
125
125
0
0
0
0
1
1
5
5
25
25
0
0
0
0
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
25
25
25
25
50
50
125
125
125
4.3
4.4
2.6
2.7
3.9
1.1
2.5
2.6
4.0
2.8
6.9
3.9
5.0
5.0
3.4
3.5"
4.3
4.4
4.3
4.6
3.9
4.3
5.6
5-8
4.1
4.4
4.9
5.5
5.9
5.6
3.9
4.3
.0
1.5
6.0
1.9
1.7
3.6
4.3
1.6
1.8
3.5
3.4
3.3
5.8
8.9
2.3
1.2
4.2
3.9
1.3
4.0
3.9
4.4
4.4
4.0
4.7
4.5
4.6
4.0
3.6
3.4
4.2
4.8
5.2
4.0
4.5
5.6
6.3
3.3
4.5
2.8
5.0
179.7
161.7
42.5
.0
209.1
79.2
42.7
43.9
49.7
7.1
7.1
532.2
613.9
520.0
217.0
220.9
121.6
67.9
290.6
363.9
106.3
7.9
7.9
7.8
8.0
7.1
8.1
7.1
7.1
6.9
7.3
6.7
6.9
7.1
7.1
9.4
5.0
7.8
7.9
6.7
7.6
57.1
784.0
34.7
46.3
82.9
55.6
70.3
36.0
38.0
33
33
11
12
14
4
10
8
13
7
30
7
34
34
36
36
36
37
38
36
35
37
37
36
33
37
33
37
204
205
62
20
306
105
69
71
71
.9
.9
.2
.4
.4
.9
.9
.9
.2
.1
.9
.5
.9
.£
.4
.9
.9
.0
.9
.9
.9
.9
.0
.0
.0
.9
.8
.5
.0
.0
.8
.8
.5
.2
.7
.3
.7
.6
.6
.6
.6
43. B
41.1
11.2
10.3
15.5
3.7
8.8
10.0
36.2
5.7
33.5
5.7
42.6
42.0
39.5
41.4
13.0
12.3
13.7
12.3
39.9
47.8
44.7
53.4
13.7
12.6
12.1
13.2
49.4
44.4
41.8
50.8
69.3
13.6
6.3
5.4
13.6
5.9
27.6
17.5
17.1
7.2
6.8
1037.6
1593.2
1394.2
414.5
1057.0
916. S
527.6
1150.0
611.7
620.1
1-4.0
13.2
19.5
20.3
14.4
16.4
14.0
13.6
9.2
9.4
8.1
7.9
12.4
12.0
16.1
23.0
27.0
29.0
9.7
9.0
99.2
105.7
78.4,
76.7
109.6
82.5
224.7
59.6
67.7
75
-------
SORTED DATA
s
s
s
5
S
S
S
s
s
s
5
S
S
5
S
S
s
s
s
s
s
s
s
s
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
T L
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
1
1
0
0
1
1
0
0
1
1
0
0
1
1
0
0
1
1
0
0
1
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
1
1
1
0
0
0
0
0
0
1
1
1
0
0
0
0
1
1
1
1
5
5
5
5
25
25
25
25
50
5Q
50
50
125
125
125
125
0
0
0
1
1
1
1
1
1
1
1
5
5
5
5
5
5
5
25
25
25
25
25
25
25
25
25
-999
-9«9
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
3
2
1
4
4
-999
2
2
2
1
3
3
2
2
4
3
1
2
3
3
1
2
a
-999
10
2
Z
5
3
6
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.6
.7
.6
.6
.6
.8
.1
.8
.0
.9
.3
.5
.6
.7
.9
.8
.4
.6
.0
.9
.3
.0
.6
.6
.3
.0
.3
.9
.4
.8
.S
.0
.0
.Z
.2
.7
6.0
7.0
5.0
6.0
7.0
8.0
8.0
7.0
5.0
7.0
7.0
6.0
5.9
8.0
8.0
7.0
7.5
6.9
6.4
4.6
11.7
9.7
13.0
10.6
-999.9
2.7
2.8
30.6
31.0
23.6
25.2
23.9
21.0
16.4
16.6
28.8
26.9
23.6
23.6
30.6
29.7
39.3
67.8
63.4
.0
.0
.0
.0
25.5
.0
49.4
10.9
12.0
11.0
11.0
10.0
10.9
10.9
11.9
9.9
11.0
10.9
12.0
10.9
10.0
15.9
8.4
8.0
8.4
8.8
8.6
10.9
10.0
12.0
11.0
15.8
13.2
13.1
22.1
21.9
20.7
20.4
14.0
16.0
14.5
14.7
21.8
25.9
21.8
21.8
15.8
13. C
16.8
32.1
29.8
.0
.0
ei.9
2
-------
SORTED OATA 8
T L
T L
T L
T L
T L
T L
T L
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T *
U I
U
U
U
U
U
U
U I
U I
U I
U I
U I
U I
U I
U I
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
0
1
1
1
1
1
0
0
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
1
1
1
1
0
0
0
0
1
1
0
0
0
0
1
1
0
0
1
1
0
0
1
1
1
25
50
50
50
50
50
50
0
0
1
1
1
1
1
1
5
5
5
5
5
5
5
5
25
25
25
25
25
25
25
25
50
50
50
50
50
50
0
0
0
0
0
0
1
1
1
1
5
5
5
5
5
10
4
6
3
8
11
10
-999
-999
1
1
-999
1
3
3
-999
1
2
2
2
3
3
2
1
1
2
2
1
1
3
2
2
2
2
2
2
2
1
-999
4
3
2
-999
3
-999
2
-999
2
2
4
.0
.2
.0
.9
.3
.2
.9
.9
.9
.0
.3
.9
.0
.4
.4
.9
.5
.9
.8
.6
.2
.3
.9
.3
.0
.0
.4
.1
.3
.0
.0
.1
.1
.9
.5
.2
.4
.0
.9
.0
.9
.2
.0
.0
.9
.7
.9
.0
.9
.6
.0
.7
.0
90.6
98.6
.0
34. 0
34.1
39.5
5.9
4.1
27.4
24.9
20.0
27.8
15.6
21.2
32.9
32.9
32.7
.0
.0
29.9
30.8
28.6
33.0
45.4
41.7
40.0
22.7
21.8
31.7
33.4
46.2
45.9
64.5
60.7
27.9
29.4
-999.9
-999.9
4.3
5.1
17.2
14.0
10.3
9.4
10.2
9.3
11.9
13.9
10.9
11.1
11.5
63
65
49
19
25
22
-999
-999
15
16
12
11
12
13
15
12
13
14
15
15
15
12
17
24
17
16
11
11
13
15
29
27
34
32
16
11
8
7
7
7
12
11
11
11
9
7
15
11
7
12
9
.9
.6
.7
.5
.3
.7
.9
.9
.7
.0
.0
.9
.2
.4
.9
.9
.4
.2
.8
.0
.4
.9
.0
.7
.9
.9
.8
.9
.8
.7
.2
.0
.8
.7
.3
.6
.9
.9
.5
.8
.1
.1
.6
.7
.6
.8
.9
.9
.9
.9
.8
75
77
59
46
37
44
-999
-999
46
40
29
27
30
30
43
43
48
50
40
45
43
40
46
50
43
42
37
33
31
46
46
56
51
33
34
16
19
13
15
36
35
21
21
27
23
39
39
27
31
23
.0
.9
.2
.0
.7
.3
.4
.9
.9
.1
.9
.9
,8
.2
.4
.6
.8
.1
.3
.4
.8
.7
.8
.9
.4
.7
.8
.5
.8
.0
.4
.2
.9
.5
.9
.8
.9
.6
.7
.9
.3
.9
.9
.4
.4
.0
.3
.8
.7
.7
.4
.9
.0
94.7
100.0
91.3
16.7
.0
19.8
15.5
15.5
28.4
28.9
20.0
21.8
20.1
21.2
31.7
27.9
24.0
25.6
15.8
.0
19.9
22.0
24.7
21.8
19.9
24.7
24.8
17.8
21.6
39.0
41.2
49.6
48.0
12.3
12.1
13.1
15.1
12.9
13.4
19.9
16.0
16.1
19.4
.0
18.7
29.0
27.4
20.9
16.6
16.2
.0
56.3
.0
53.1
26.5
.0
31.1
27.2
23.6
35.3
33.0
28.9
35.2
30.8
32.9
37.7
30.4
34.9
35.5
34.5
39.4
37.2
36.3
25.0
34.8
32.8
44.9
27.2
27.3
28.7
30.4
34.9
28.8
45.8
39.1
29.8
25.9
50.0
39.4
49.0
47.2
.6
48.2
63.1
59.3
^4.5
44.9
69.6
74.4
34.8
32.5
35.3
77
-------
SORTED DATA
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
M
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
I
I
I
I
I
I
s
s
s
5
s
s
s
s
5
s
s
s
s
s
s
5
s
s
s
s
s
s
5
s
s
s
s
1
1
1
1
I
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
i
1
1
1
1
1
0
0
0
0
1
1
1
1
0
0
1
1
1
1
0
0
0
0
0
0
1
1
1
1
0
0
1
1
0
0
0
0
0
1
1
0
0
1
1
1
1
0
0
1
1
0
0
1
1
0
0
5
25
25
25
25
25
25
25
25
50
50
50
50
50
50
125
125
125
125
125
125
125
125
125
125
0
0
0
0
1
1
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
50
50
50
50
125
125
4
3
3
6
ft
3
3
5
5
2
2
3
3
4
4
2
2
4
4
4
4
3
3
6
6
-999
3
3
3
-999
2
5
5
5
-999
-999
-999
-999
3
-999
5
5
3
3
3
4
4
4
5
4
4
4
.5
.0
.4
.3
.8
.4
.3
.7
.9
.2
.4
.5
.4
.6
.6
.6
.3
.6
.6
.5
.3
.4
.7
.1
.2
.9
.5
.5
.1
.9
.8
.4
.8
.1
.9
.9
.9
.9
.9
.9
.5
.2
.9
.9
.7
.3
.4
.1
.0
.7
.8
.7
10.8
27.9
29.9
23.5
18.6
14.0
13.5
9.7
9.7
36.6
36.4
27.9
29.5
34.7
34.7
22.9
18.9
19.3
22.6
18.6
19.6
7.6
6.5
8.2
6.7
4.4
4.9
4.9
4.8
4.4
3.8
2.9
3.3
3.4
3.6
3.8
5.3
5.9
3.4
3.6
2.6
2.8
3.5
3.5
2.8
3.6
6.5
6.6
6.5
6.5
6.5
6.5
9.5
22.0
25.9
20.2
19.9
14.0
19.3
10.5
11.1
24.9
27.0
20.0
19.0
17.3
17.4
27.9
22.9
19.0
19.3
18.7
19.4
7.1
8.0
9.0
8.8
1.0
4.3
4.1
4.1
1.0
1.0
4.5
4.2
3,9
1.0
1.0
.9
1.0
4.6
.9
4.4
4.2
6.1
5.1
4.4
5.1
6.2
6.2
6.2
6.2
6.6
5.3
23.6
39.9
45.9
36.6
35.8
21.9
.0
23.2
23.0
53.8
57.9
50.7
51.0
44.7
44.6
18.9
17.0
26.1
25.4
24.8
24.8
18.9
19.0
16.5
16.3
1.0
22.3
22.9
22.3
8.5
3.9
7.0
16.4
14.7
3.9
2.5
5.2
4.0
.8
7,&
15.6
17.6
30.9
32.9
30.9
35.«
28.9
27.8
29.8
27.9
27.8
28.0
16.7
37.9
37.1
39.6
39.8
16.6
16.2
14.0
16.0
29.5
32.7
20.3
20.4
19.9
19.8
55.9
46.6
50.0
49.8
49.6
49.7
21.0
19.6
20.4
20.4
34.9
52.5
43.0
42.0
56.8
55.1
37.9
35.6
37.9
64.1
62.0
56.2
56.5
44.8
49.1
38.1
39.2
12.8
11.4
10.5
10.1
57.8
57.8
60.7
56.0
57.7
59.0
40.3
91.5
92.1
85.9
94.1
53.9
50.2
40.6
44.9
92.7
96.8
.0
.0
77.4
77.3
166.4
207.0
172.9
187.8
179.1
171.2
89.9
114.6
123.2
118.2
11.3
11.6
12.4
11.6
11.5
9.4
8.4
26.2
22.2
12.4
12.5
10.4
12.7
12.4
10.1
8.3
8.4
10.0
11.0
13.0
13.4
14.9
15.9
15.9
14.9
16.4
16.0
78
-------
SORTED DATA
10
u :
U !
z c
Z I
Z [
Z E
Z I
c
c
c
c
c
c
c
c
c
c
c
c
c
c
C 1
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
c
C !
c «
C «
c .
c .
c .
> 1
> 1
) 1
) 1
) 1
) 1
) 1
2
2
2
2
2
2
2
2
2
2
[ 2
[ 2
[ 2
[ 2
[ 2
[ 2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
5 2
5 2
& 2
S 2
S 2
S 2
1
1
0
1
0
1
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
c
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
125
125
25
25
50
50
50
0
0
0
1
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
125
125
125
0
0
0
1
1
1
4.4
4.7
4.9
5.7
2.3
2.9
.0
2.2
2.0
2.2
1.1
1.1
1.2
1.3
1.2
1.0
1.3
1.6
1.1
1.4
1.5
2.2
1.9
2.0
1.6
1.6
1.6
1.5
1.6
1.6
1.5
1.8
1.8
1.7
1.5
1.3
1.4
1.0
1.0
1.1
2.4
2.3
2.5
1.6
1.4
1.5
3,6
3.5
4.5
.0
2.4
2.2
5
5
185
260
10
97
230
3
3
3
2
2
1
1
1
1
3
4
2
1
3
4
4
3
3
4
4
4
4
4
4
4
4
3
7
6
7
6
5
6
3
3
4
4
3
3
8
7
5
6
7
6
.4
.4
.4
.8
.0
.5
.3
.8
.8
.6
.0
.0
.9
.8
.9
.9
.9
.8
.8
.6
.8
.5
.4
.8
.8
.8
.8
.8
.2
.2
.1
.1
.1
.5
.4
.5
.5
.5
.4
.4
.7
.7
.6
.7
.3
.2
.0
.8
.7
.4
.6
.7
4.9
5.0
74.6
89.7
19.2
42.9
77.4
21.9
20.7
22.9
20.8
20.3
19.3
26.7
27.2
28.5
24.4
26.7
18.1
14.2
17.5
16.8
20.6
18.8
21.2
12.5
14.4
12.8
20.0
20.8
19.3
13.5
14.5
11.8
23.4
21.2
22. C
23.2
21.0
27.2
27.2
26.1
29.0
12.9
11.1
11.2
7.6
7.6
7.3
7.6
8.1
7.8
25.9
28.0
201.8
248.1
49.5
113.1
216.3
7.2
6.7
6.5
6.0
5.6
5.8
6.4
6.1
5.8
5.0
5.8
4.8
4.0
4.3
5.0
5.9
5.7
6.1
6.4
6.6
7.0
3.9
4.0
3.6
3.6
3.8
3.8
6.6
6.3
6.2
6.0
5.7
5.7
6.3
5.2
6.1
5.9
5.0
5.9
11.9
12.4
14,8
15.6
15.6
14.2
52
51
15
15
7
47
47
-999
5
5
5
4
3
3
3
4
5
5
7
2
4
8
5
5
5
5
4
4
6
4
4
3
3
2
4
5
5
4
5
5
4
4
4
2
4
3
33
35
32
38
37
35
.0
.2
.7
.3
.8
.2
.2
.9
.9
.0
.0
.0
.8
.7
.9
.4
.8
.8
.5
.8
.8
.1
.0
.0
.7
.8
.8
.8
.9
.0
.8
.8
.6
.e
.8
.9
.4
.9
.8
.3
.0
.1
.2
.7
.1
.2
.8
.5
.6
.6
.1
.1
13.5
13.5
126.7
67.0
30.4
46.8
.0
111.7
126.0
121.4
152.1
151.9
147.5
112.7
104.1
106.1
124.1
126.7
105.6
65.8
83.0
119.4
95.7
79.2
95.1
85.4
94.4
99.2
98.0
90.6
113.4
66.2
61.4
56.7
109.7
101.5
107.4
103.0
97.1
109.7
86.7
87.8
97.8
140.6
124. S
131.1
2.9
2.3
2.6
2.4
3.6
1.8
79
-------
SORTED
C S
C S
C S
C S
C 5
C S
C S
C S
C S
C S
C S
C S
C S
C 5
C 5
C 3
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C S
C 5
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H 8
H B
H B
H B
H B
H B
H B
H B
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
50
50
50
50
50
50
85
65
85
85
95
85
125
125
125
125
125
125
0
0
0
1
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
2
2
2
2
2
2
4
2
2
3
2
2
2
2
2
1
2
2
2
2
2
2
2
2
2
2
2
2
I
2
2
DATA
.6
.1
.1
.5
.9
.6
.0
.9
.7
.2
.8
.0 *
.8
.3
.9
.0
.4
.8
.2
.1
.0
.0
.0
.0
.2
.0
.0
.7
.8
.7
.5
.8
.8
.8
.1
.0
if if if it £##
i
1
1
1
1
1
iftftftftf
1
1
1
.8
.5
.4
.4
.6
.2
.1
**
.5
.6
.3
.4
.8
.7
.7
6
7
7
7
6
6
7
7
7
o«#«
8
7
7
7
6
8
7
6
7
9
9
9
7
8
10
6
6
7
6
5
2
3
2
2
2
2
1
1
1
2
3
3
4
4
4
5
4
5
3
11
.7
.4
.6
.6
.8
.5
.1
.2
.4
.0
.0
**
.6
.6
.3
.4
.4
.0
.3
.8
.8
.0
.8
.0
.7
.8
.1
.5
.0
.5
.0
.0
.9
.8
.0
.8
.0
.2
.2
.4
.8
.4
.8
.8
.9
.8
.9
.5
.7
.5
.1
.0
8.7
8.4
8.9
9.0
7.8
7.7
7.7
7.8
8.0
7.8
8.3
7.8
7.9
8.2
.0
5.6
5.1
5.6
5.5
5.2
5.5
7.1
6.7
6.7
6.2
6.7
7.7
6.8
6.9
7.0
6.6
5.9
5.9
2.7
2.8
2.6
2.9
2.8
2.7
2.4
2.6
2.5
2.9
3.2
3.0
3.2
3.2
3.0
9.7
2.8
2.8
2.5
15.2
13.8
17.1
11.4
12.6
12.3
12.4
14.4
12.0
14.4
13.8
12.8
13.3
13.3
13.7
8.2
8.9
7.3
8.1
8.1
8.0
15.2
14.5
14.6
16.0
13.9
13.9
18.0
18.4
17.6
15.8
16.8
18.7
6.4
.0
7.0
6.5
6.2
5.8
5.6
5.5
6.0
6.0
6.2
«»££**»
.0
7.5
6.9
1.3
5.0
4.7
3.5
36.2
34.0
47.6
34.3
35.9
35.2
35.5
37.8
34.1
35.2
34.9
34.2
.0
36.0
33.8
46.2
44.6
47.1
49.3
48.7
47.2
45.0
49.2
50.2
50.0
46.1
47.6
.0
44.8
42.1
45.7
44.4
45.0
11.1
12.6
10.0
11.7
11.4
11.2
7.6
9.2
9.0
11.4
11.6
10.7
11.5
13.6
9.9
.0
10.4
11.4
12.7
3.0
3.0
4.5
1.0
1.4
1.6
1.6
1.6
1.6
2.8
6.1
5.9
2.0
2.6
2.8
3.2
3.6
4.1
2.2
3.0
3.7
2.8
1.6
3.1
2.0
2.5
1.4
4.4
2.5
2.9
5.3
4.1
2.6
5.0
5.4
5.4
4.7
4.7
5.0
4.2
4.2
4.6
4.2
4.5
4.3
4.6
4.2
3.5
10.0
8.9
8.4
6.4
80
-------
SORTED DATA
12
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H B
H 6
H
H
H
H
H
H
H
H
H
H
H
H I
H I
H I
H I
H I
H I
H I
H I
H I
H 1
H I
H I
H I
H I
H
H
H
H
H
H
H
H
2
2
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
25
25
50
50
50
50
50
85
85
85
85
G5
85
125
125
125
125
125
125
0
0
0
1
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
1
1
*****
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
4
1
1
1
1
1
1
.4
.3
.8
.1
.8
.0
.9
**
.8
.7
.7
.7
.7
.8
.8
.8
.6
.6
.6
.1
.0
.3
.5
.5
.5
.6
.8
.7
.6
.4
.6
.4
.3
.1
.9
.9
.0
.0
.0
.0
.9
.8
.8
.8
.9
.4
.4
.4
.9
.8
.0
.8
12.7
11.8
4.6
4.7
3.5
4.1
3.9
5.2
6.0
4.9
.5
6.0
5.4
8.0
9.2
9.7
1.2
.8
1.5
15.0
13.6
14.4
7.4
7.2
7.0
19.7
21.7
21.6
36.2
37.9
41.0
46.3
46.6
45.6
44.7
45.3
32.4
34.7
35.3
84.2
82.6
89.7
44.0
46.0
42.8
94.6
93.2
89.1
75.7
78.6
78.8
50.5
5.7
4.6
4.9
4.3
3.7
4.3
4.7
3.6
3.8
3.2
2.7
3.2
3.1
5.1
5.1
5.1
2.6
2.5
2.6
7.6
7.6
7.6
5.4
5.5
5.2
8.5
9.2
9.0
13.8
13.6
14.6
16.3
16.8
16.7
14.9
14.5
9.7
10.2
10.4
19.0
18.9
22.9
15.0
15.5
14.2
23.6
23.8
23.9
14.0
13.1
13.2
12.8
6.5
5.2
5.4
5.9
5.4
6.0
6.5
6.2
6.3
6.0
6.6
6.6
6.6
11.6
11.1
11.1
5.7
5.8
6.7
20.5
20.0
19.4
26.7
25.6
25.0
6.8
6.4
6.2
74.2
72,6
79.2
36.2
36.2
40.0
32.3
33.8
29.3
30.9
.0
65.9
63.4
78.7
27.1
28.5
26.9
4.1
40.2
39.8
34.3
36.2
36.7
36.3
-999
-999
8
11
13
24
15
13
13
13
12
13
13
7
7
7
7
4
4
30
26
23
9
8
9
9
9
•9
17
15
15
13
\2
15
25
23
-999
13
14
12
10
10
8
6
8
49
48
46
15
16
17
15
.9
.9
.2
.7
.8
.3
.6
.7
.6
.4
.8
.9
.7
.2
.2
.2
.2
.2
.7
.3
.7
.3
.5
.9
.6
.2
.5
.5
,2
,0
.6
.1
• 5
.8
.2
.6
.9
.5
.7
.4
.4
.1
.2
.4
.2
.9
.3
.4
.8
.6
.0
.5
9.9
7.1
9.4
6.7
5.7
6.7
7.1
7.2
7.0
5.9
7.1
6.8
6.1
22.5
17.2
18.3
7.2
15.3
7.2
28.0
26.2
26.7
39.9
39.3
39.3
43.8
48.2
48.9
56.1
51.9
65.6
45.8
48.9
47.0
47.5
.0
40.0
41.5
44.1
35.1
40.0
40.3
29.8
27.9
28.6
67.7
64.1
62.2
40.5
45.2
39.6
50.0
81
-------
SORTED DATA
H
H
H
H
H
H
H
H
M
H
H
H
M
H
M
H
H
H
H
M
H
M
H
M
H
M
H
H
H
H
N
H
H
H
M
M
M
H
H
M
H
H
H
H
H
H
H
H
H
H
M
M
I
I
I
I
I
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
B
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
L
L
L
L
L
L
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
0
0
1
1
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
125
125
125
125
125
0
c
0
5
5
5
5
5
5
50
50
50
50
50
50
125
125
125
125
125
125
0
0
0
5
5
5
5
5
50
50
50
50
50
50
125
125
125
125
125
125
0
0
0
5
5
5
.8
.8
1.2
1.0
1.0
.3
.2
.3
.4
.4
.4
.6
1.4
1.7
.1
.1
.2
.2
.1
.2
.3
.2
.3
.4
.3
.3
.3
.4
.5
.5
.6
.8
.7
.6
.1
.1
.2
.1
.1
.2
.4
.4
.4
.4
.5
.2
.4
.4
.3
.7
.6
.9
13
50.1
52.9
28.2
34.4
28.4
6.9
6.8
6.9
6.7
5.4
6.7
10.6
3.1
3.0
5.0
4.8
4.7
4.5
4.5
4.5
6.8
7.1
7.3
6.6
6.5
6.7
2.5
2.5
1.8
6.8
8.9
10.0
10.5
8.7
2.0
2.0
1.6
1.6
1.1
1.9
4.0
3.6
3.3
3.8
3.5
2.7
3.3
3.2
2.9
2.2
2.0
2.1
12.6
13.6
9.7
10.8
9.1
4.7
6.1
4.6
5.7
5.0
' 5.2
5.9
3.2
3.0
4.1
5.2
4.3
4.8
5.4
5.4
5.1
3.8
3.8
4.2
4.1
4.1
4.7
5.4
5.3
8.2
7.9
6.6
7.3
6.6
6.6
6.9
5.9
7.4
7.8
7.2
9.1
8.4
7.1
7.8
4.0
6.2
6.3
5.4
5.9
13.2
12.1
10.2
34.8
35.8
.0
21.2
19.5
9.1
9.8
10.2
9.9
6.9
8.6
7.3
5.3
6.6
3*. 3
3.5
3.6
3.7
3.4
3.8
4.3
3.8
4.3
6.1
5.3
4.8
4.8
4.3
4.9
11.3
14.6
5.6
.0
6.0
2.2
3.2
2.4
2.9
3.2
2.5
4.0
3.9
4.5
4.6
4.2
3.8
5.9
5.9
5.8
5.6
7.9
6.3
13.6
13
6
7
7
2
-999
-999
-999
4
2
-999
8
9
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
-999
5
5
-999
7
6
-999
-999
-999
-999
-999
-999
-999
-999
-999
9
-999
-999
-999
-999
4
3
-999
4
.5
.9
.2
.2
.1
.9
.9
.9
.4
.9
.9
.5
.8
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.9
.0
.9
.9
.6
.3
.9
.9
.9
.9
.9
.9
.9
.9
.9
.2
.9
.0
.9
.9
.9
.9
.4
.9
.0
7.9
4
.5
49.7
51.5
41.6
37.8
40.3
2.7
2.8
2.9
4.2
3.8
4.2
5.2
4.4
7.2
4.2
.0
2.7
4.3
2.7
.0
3.7
2.8
4.4
4.5
5.0
4.9
5.0
4.7
4.9
7.7
8.9
10.8
16.5
16.2
4.8
4.6
3.5
5.6
4.7
5.3
7.1
8.4
7.8
9.5
7.7
10.4
16.2
17.2
14.0
10.1
10.9
9.3
82
-------
SORTED
N
M
H
H
H
M
M
H
M
M
H
H
M
H
N
N
N
N
H
N
N
N
M
H
H
H
M
H
H
H
H
H
H
H
H
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
L
L
L
L
L
L
L
L
L
L
L
L
L
L
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
I
I
I
I
I
S
S
S
S
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
1
0
1
0
1
0
1
0
1
0
1
1
1
0
5
5
50
50
50
50
50
50
125
125
125
125
125
125
0
0
0
5
5
5
5
5
50
50
50
50
50
50
125
0
125
125
125
125
0
0
0
1
1
5
5
25
25
50
50
85
85
125
0
0
0
1
1
-999
-999
1
1
1
1
4
3
3
1
1
1
1
1
1
4
2
2
1
3
4
3
3
3
DATA
*
•
•
*
*
*
*
•
*
*
*
*
»
•
*
*
•
•
•
•
•
*
*
*
•
*
.
•
•
*
*
*
*
*
•
•
•
»
»
*
•
•
*
»
*
•
*
*
•
*
•
*
0
6
1
9
9
1
1
1
3
4
4
4
5
5
0
6
0
6
5
5
0
0
3
3
4
3
3
4
7
0
7
6
8
0
5
6
3
4
6
8
8
9
7
4
3
6
5
8
2
8
3
0
14
12.5
7.8
1.4
2.1
2.0
1.6
1.1
2.0
2.2
2.1
1.4
3.6
3.7
3.5
26.4
26.6
20.1
17.7
17.3
17.7
17.4
15.5
18.3
15.3
17.0
8.2
7.6
7.4
30
30
30
6.8
7.3
7.5
6.4
6.2
6.1
3.3
3.1
2.6
1.6
2.7
2.9
5.2
7.4
4.8
10.0
6.3
7.0
8.8
7.8
7.3
.5
.2
,9
8.7
6.4
8.9
9.7
9.2
7.8
7.5
7.4
8.7
8.6
9.1
9.2
10.1
9.1
10.2
10.6
10.3
10.0
9.9
9.6
9.7
6.3
10.4
9.7
10.7
5.8
6.0
5.8
6.9
6.6
8.0
6.0
6.0
6.4
48.9
38.6
49.2
11.3
9.6
53.6
61.9
43.3
49.0
67.8
64.8
100.9
74.9
57.7
6.7
7.4
7.3
7.9
8.2
6.3
2.7
2.6
2.7
2.4
2.2
2.8
2.4
2.4
2.5
4.5
4.8
5.0
31.3
33.6
32.8
26.4
27.2
26.3
16.4
17.4
11.2
9.5
10.7
4.4
4.8
4.2
14.1
14
11
12
13
11
10
12
7
8
9
6
6.0
5.8
15.7
13.8
.3
.9
.6
.8
.2
.4
.0
.3
.7
.0
.5
.0
.6
.8
,9
7,
11
10.3
13.0
11.6
11.7
11.9
-999.9
-999.9
-999.9
-999.9
-999.9
-999.9
2.2
6.1
-999.9
4.0
-999.9
-999.9
-999.9
-999.9
4.1
4.7
3.9
4.6
5
5,
-999.9
3.9
-999.9
4.6
2.8
1.0
9.9
4.8
7.3
7.2
7.4
-999.9
-999.9
4.1
15.6
16.3
15.8
7.9
9.3
7.0
8.8
7.7
8.2
18.3
10.6
8.7
8.5
11.6
36.4
35.8
35.8
33.8
11.0
13.4
10.9
14.2
8.1
10.9
8.9
8.7
7.3
7.8
7.6
16.1
11.5
11.2
.0
25.0
20.2
20.7
21.3
20.7
17.0
17.1
16.7
15.6
.0
13.0
12.8
11.5
20.5
20.3
26.2
17.5
20
18
159.6
113.6
170.7
112.2
128.1
127.5
176.3
132.7
176.7
193.8
107.4
120.6
142.8
123.2
5.1
5.0
4.8
4.9
,2
,5
83
-------
SORTED DATA
N S 2
N S 2
N 5 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N 5 2
N S 2
N S 2
M S 2
N S 2
N S 2
N S 2
N S 2
N 5 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N S 2
N 5 2
N S 2
N S 2
0 I 2
Q I
0 ]
0 1
Q 1
Q 1
0 ]
Q 1
0 1
0
Q
D
0
0
D
0
a
Q
0
D
2
[ 2
2
[ 2
2
I 2
2
2
2
2
2
2
2
2
2
2
2
2
2
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
c
0
1
1
1
0
c
1
1
0
0
0
1
1
1
0
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
0
0
0
1
1
1
1
1
1
5
5
5
5
25
25
25
25
25
25
50
3
3
3
3
3
4
3
3
3
4
3
3
3
3
3
2
3
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
5
5
A
2
2
3
A
4
3
3
5
5
6
3
4
3
3
.0
.0
.5
.9
.0
.0
.8
.8
.7
.5
.7
.0
.3
.2
.1
.8
.6
.0
.3
.1
.4
.2
.0
.2
.2
.3
.4
.3
.3
.4
.4
.5
.7
.8
.8
.0
.0
.7
.7
.6
.1
.6
.7
.6
.8
.6
.3
.8
.7
.4
.9
.8
15
7.9
8.6
7.8
9.9
7.8
7.2
6.2
5.7
7.8
6.8
6.6
7.6
7.4
7.9
9.0
7.7
8.9
8.3
7.7
8.6
7.0
8.0
8.6
8.8
9.3
9.5
8.6
9.5
8.7
9.5
9.7
9-7
10.8
9.8
10.6
6.3
6.1
5.3
9.5
10.2
17.6
11.6
14.3
9.3
11.3
18.5
19.5
25.7
13.6
15.1
17.2
29.8
8
8
8
8
8
6
6
6
7
6
6
8
8
8
8
8
8
10
10
10
9
9
10
9
9
9
8
9
9
8
7
8
88
85
91
161
152
162
93
95
68
157
137
99
98
214
217
238
146
155
137
214
.7
.3
.1
.2
.4
.3
.2
.0
.1
.4
.4
.6
.1
.4
.5
.0
.4
.2
.2
.3
.3
.7
.7
.4
.1
.3
.9'
.9
.4
.0
.6
.2
.6
.5
.5
.6
.7
.C
.5
.7
.2
.1
.1
.7
.7
.9
.5
.4
.0
.6
.6
.2
11.9
11.4
13.6
13.6
11.6
13.4
11.6
13.2
10.7
13.6
12.3
11.0
12.0
11.0
10.8
11.1
12.5
14.4
14.9
13.6
16.2
15.3
14.6
13.4
13.6
13.7
14.1
13.6
13.5
12.2
12.6
13.2
18.9
17.9
20.9
12.9
12.1
11.9
20.5
21.8
33.4
20.2
23.8
17.4
20.6
2p.7
24.8
33.8
17.0
19.7
17.2
25.9
36.7
37.0
37.1
37.5
34.9
34.0
35.6
34.0
35.7
34.0
31.1
32.1
34.3
31.5
32.4
33.4
33.9
48.2
49.2
48.7
46.7
45.9
46.9
50.9
50.6
50.0
45.4
49.3
52.1
39.3
45.5
43.7
4.9
6.0
10.8
14.8
11.3
11.3
9.6
5.9
7.2
8.9
10.5
8.2
10.0
13.1
16.5
14.3
11.2
9.1
4.7
10.9
7.6
4.8
4.3
7.3
4.8
4.2
5.1
4.0
3.6
5.0
4.8
4.6
4.4
4.0
4.0
4.2
5.1
4.6
4.1
4.7
4.7
4.2
4.8
3.0
3.2
3.7
3.8
5.5
3.9
4.4
5.1
4.9
563.4
825.5
583.2
933.8
913,0
915.6
513.0
539.3
433.6
676.1
609.3
491.7
568.7
816.8
839.8
997.7
761.8
71 3. •
662.2
842.4
84
-------
SORTED DATA 16
6.4 741.0
4.3 678.0
23.7 2386.9
7.0 735.2
16.3 1096.0
12.4 961.2
5.1 391.5
38.6 4.0
38.2 3.7
37.7 3.1
36.9 3.8
35.6 4.2
35.7 4.1
35.3 8.9
37.4 9.5
37.4 11.8
34.0 10.0
31.9 9.1
35.4 7.0
34.5 7.4
33.7 5.9
35.7 6.8
31.4 5.7
32.5 5.5
37.7 5.9
32.0 6.4
32.1 6.5
42.0 7.2
48.1 6.8
44.8 7.3
50.2 7.3
47.1 7.4
47.8 6.6
44.8 6.8
48.6 16.8
51.0 18.4
51.1 20.2
51.0 15.3
50.2 15.3
51.6 16.5
45.2 16.5
41.3 17.1
34.7 16.4
37.6 11.6
35.6 10.4
44.4 10.6
35.2 74.3
36.5 72.9
39.5 79.3
15.0 70.0
12.0 80.0
13.0 86.2
85
0
0
0
0
D
0
0
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
D S
0 S
0 S
0 S
a s
Q 5
0 S
0 S
D S
0 S
0 S
0 S
0 S
0 S
0 S
0 S
0 5
Q S
Q S
Q S
Q S
0 S
o s
0 S
0 S
0 S
P D
P D
P D
P D
P D
P D
2
2
2
2
2
2
2
2
0
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
0
1
0
0
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
50
50
85
85
125
125
125
0
0
0
1
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
125
125
125
25
25
25
25
25
25
3
2
7
2
3
3
2
3
3
3
3
3
3
2
3
3
3
2
3
3
3
3
3
3
2
2
2
2
1
1
1
2
1
1
1
1
1
1
1
1
2
2
2
2
2
2
1
1
1
1
1
1
.1
.0
.4
.8
.6
.3
.5
.0
.1
.1
.2
.1
.0
.6
.3
.2
.1
.6
.4
.1
.1
.9
.2
.2
.9
.6
.8
.7
.7
.9
.9
.0
.6
.8
.7
.7
.5
.7
.8
.9
.3
.3
.4
.4
.3
.4
.8
.8
.9
.6
.5
.5
19
13
18
7
24
32
17
7
7
7
10
8
8
12
11
11
13
12
12
13
12
13
12
11
12
16
16
14
10
9
10
9
10
9
12
12
13
11
12
12
24
25
23
21
21
21
438
397
499
379
383
376
.7
.9
.4
.0
.4
.3
.2
.3
.6
.5
.2
.7
.7
.5
.0
.0
.2
.8
.7
.4
.5
.5
.7
.9
.3
.4
.0
.0
.4
.5
.6
.6
.3
.2
.4
.5
.7
.0
.1
.8
.1
.6
.3
.0
.9
.0
.5
.4
.8
.3
.5
.7
129
119
720
299
300
372
244
6
6
6
9
7
7
8
8
7
9
9
9
9
9
10
6
7
8
9
9
8
7
7
7
7
7
7
13
12
13
10
11
11
10
11
9
9
9
9
47
48
51
52
49
55
.2
.1
.8
.3
.1
.7
.5
.9
.8
• 8
.2
.8
.4
.2
.0
.8
.6
.6
.6
.8
.6
.2
.1
.9
.4
.1
.4
.6
.4
.4
.9
.5
.6
.4
.0
.7
.6
.3
.2
.3
.4
.1
.6
.9
.7
.7
.8
.3
.3
.2
.8
.2
17
15
25
11
22
28
16
17
19
18
17
16
17
20
21
21
18
15
19
18
18
21
14
14
16
20
16
18
12
13
14
14
12
13
14
14
15
13
14
15
25
23
23
20
20
21
338
318
354
262
288
296
.3
.6
.0
.0
.4
.4
.9
.8
.6
.4
.0
.8
.8
.8
.5
.5
.0
.9
.0
.2
.3
.2
.4
.3
.4
.1
.0
.1
.8
.9
.1
.0
.7
.4
.6
.3
.2
.0
.7
.8
.0
.6
.8
.8
.8
.7
.0
.6
.5
.5
.6
.7
-------
SORTED DAT
P D
P D
P D
P D
P D
P D
P 0
P D
P D
P D
P D
P D
P D
P D
P D
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
5
S
5
S
5
S
S
S
5
S
S
S
S
S
S
S
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Q
0
0
0
0
0
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
0
0
0
1
1
1
5
5
5
25
25
25
50
50
50
85
85
85
125
125
125
0
0
0
0
0
0
1
1
1
1
1
1
5
5
5
5
2.0
2.0
2.3
1.4
1.6
1.8
1.9
1.9
1.9
2.3
1.7
1.8
1.9
2.0
1.9
264.6
118.9
114.4
57.7
50.6
66.7
63.3
53.3
60.3
56.4
45.5
52.8
62.0
61.4
76.1
40.2
30.0
23.2
16.8
18.8
18.3
.6
.6
.6
.6
.6
.6
.6
.6
.5
.6
.5
.6
.3
.3
.2
.3
A
498
532
539
429
437
425
375
395
390
270
188
232
218
204
229
3287
3774
3938
1449
1374
1878
2022
1763
2152
1993
1492
2052
2057
1815
2064
1290
1239
1230
668
653
654
6
6
7
7
7
6
6
5
5
7
6
7
5
5
5
6
17
.0
.3
.0
.6
.0
.1
.9
.3
.1
.4
.5
.5
.2
.1
.9
.6
.8
.2
.5
.4
.8
.2
.2
.9
.1
.6
.2
.0
.3
.9
.6
.7
.1
.1
.9
.2
.6
.8
.1
.5
.1
.9
.0
.5
.6
.0
.8
.1
.2
.9
.9
.8
55
55
58
50
64
60
86
86
85
76
69
79
46
44
47
2249
2748
2337
1310
1230
1581
994
1058
995
1185
1652
1082
1445
1414
1489
888
873
891
522
547
438
6
6
7
5
6
5
6
6
6
6
7
6
6
7
6
6
.9
.4
.8
.8
.1
.2
.5
.1
.6
.2
.3
.3
.3
.7
.8
.8
.8
.4
.5
.9
.8
.6
.4
.5
.6
.7
.1
.3
.3
.3
.5
.9
.2
.3
.2
.0
.7
.6
.1
.7
.0
.8
.4
.3
.4
.7
.4
.5
.3
.1
.2
.4
327.7
357.1
361.6
284.0
293.1
300.0
270.5
267.0
266.7
235.4
214.0
242.2
226.5
225.5
23 IJO
364.3
373.2
389.3
276.6
244.6
317.0
328.7
307.0
337.5
254.0
224.3
258.0
233.7
215.4
239.7
254.4
239.6
219.4
139.1
149.1
130.5
7.1
7.2
7.5
9.6
9.5
9.0
6.2
6.1
6.1
8.2
5.4
5.3
4.3
4.0
4.6
6.6
44.0
45.6
.0
10.6
10.7
13.8
83.5
81.2
82.1
29.7
29.9
30.8
20.8
19.4
19.9
19.6
25.2
19.4
19.8
26.4
16.9
22.9
16.9
14.7
49.3
32.5
20.3
61.7
58.7
51.1
66.5
70.0
96.2
19.9
19.9
19.9
6.6
6.6
6.7
7.6
7.6
8.0
7.2
6.7
6.9
6.7
6.8
6.5
5.0
5.5
5.2
6.9
88.9
86.1
75.0
75.3
90.1
73.8
83.1
82.8
85.0
47.9
43.9
51.4
62.3
57.7
62.4
2446.7
2648.2
2427.9
1151.2
1065.0
1365.2
1463.1
944.0
1377.6
1378.7
933.1
1305.1
1474.0
1360.0
1499.7
914.0
879.4
778.1
436.0
451.6
406.6
16.2
16.0
16.2
15.9
16.3
16.4
15.0
15.0
15.6
16.9
15.6
15.3
12.0
13.6
16.4
12.7
86
-------
S
S
S
S
S
S
S
S
s
s
s
s
s
s
s
s
s
s
s
s
s
s
s
s
s
s
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
1
1
c
0
0
1
1
1
0
0
1
1
1
0
0
0
1
1
1
0
5
5
25
25
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
125
125
125
0
0
0
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
50
50
50
50
50
50
85
DATA
.4
.2
.5
.4
.5
.4
.5
.5
.3
.3
.3
.6
.3
.3
.2
.2
.3
.2
.2
.3
.4
.4
.3
.4
.4
.3
1.2
1.1
1.0
2.1
1.5
1.5
1.6
1 .3
2.2
1.9
2.8
1.9
2.0
2.3
4.1
4 .3
3.6
3.8
3.5
5.1
5.9
6.4
2.7
2.7
2.7
3.4
18
8.0
5.4
7.5
6.7
6.7
7.5
7.8
8.3
7.1
6.5
7.3
7.3
6.3
6.1
43.6
39.4
43.5
42.7
41 .6
43.7
54.2
54.2
52.0
54.0
50.2
51 .8
16.6
16.3
15.5
16.4
15.6
14.2
12.4
13.9
51.0
49.6
59.3
37.5
41.0
39.3
32.2
31.8
37.5
37.1
37.5
35.4
38.8
44.4
57.4
58.5
51.9
116.9
7.0
5.0
9.6
8.9
8.9
10.0
9.6
9.8
8.7
8.6
9.5
8.9
7.8
7.8
8.0
7.6
8.0
7.6
7.3
7.6
8.4
6.6
6.6
7.8
7.2
7.6
7.8
7.4
' 7.5
10.4
11.6
10.6
10.2
10.1
18.6
18.5
19.8
15.0
15.3
15.4
19.7
20.6
22.4
22.8
22.5
26.9
30.4
34.5
24. 8
24.9
25.0
29.3
6.6
4.7
7.4
6.7
7.1
8.9
8.8
8.9
7.7
7.5
8.7
7.5
7.1
7.3
8.1
7.6
8.4
8.2
8.0
8.8
11.8
11.1
10.9
10.5
,1
.5
,7
10,
10
30,
28.1
29.4
24.0
24.6
24.0
21.2
20.2
55.2
55.4
59.3
40.9
42.9
42.9
28.6
30.4
42.0
41.5
41.7
23.5
27.5
30.3
44.1
44.2
44.4
73.6
7.5
5.2
7.6
6.5
6.7
6.6
6.8
7.3
5.5
5.1
6.0
5.3
4.5
4.9
7.1
6.8
7.7
6.8
6.4
7.1
8.5
8.1
7.9
7.3
7.1
7.1
9.7
9.7
10.8
12.5
11.6
12.5
7.1
6.1
12.4
14.6
15.8
10.2
9.3
8.9
8.8
6.9
-999.9
-999.9
-999.9
13.1
13.4
14.8
9.5
9.8
11.0
6.3
14.8
13.9
15.3
14.5
13.7
15.5
15.3
16.9
16.0
15.2
18.6
14.1
10.9
11.2
15.4
15.1
14.9
16.3
15.5
14.6
13.2
17.4
17.0
16.1
15.0
15.2
28.3
26.6
28.0
25.2
26.9
25.4
28.0
24.4
34.9
40.1
37.0
39.3
36.9
33.4
23.6
22.8
33.0
32.6
30.6
41.8
45.7
53.7
39.2
41.4
40.5
31.3
87
-------
SORTED DATA 19
T L
T L
T L
T I
T L
T L
T L
T L
T L
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
T R
U I
U I
U I
U I
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
0
0
1
1
1
0
1
1
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
85
85
85
65
85
125
125
125
125
0
0
0
1
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
125
125
125
0
0
0
1
3.6
3.4
2.5
2.7
2.7
3.5
1.2
1.2
1.2
1.3
1.1
1.2
1.2
1.3
1.2
1.2
.9
1.1
1.1
1.1
1.1
1.0
1.0
1.2
1.4
1.5
1.5
1.1
1.0
1.0
1.5
1.2
1.4
1.2
1.4
1.1
1.7
1.5
1.7
1.9
1.8
1.9
1.8
1.8
1.9
1.6
1.8
1.6
2.1
2.2
2.2
2.0
128.4
129.3
43.1
49.5
42.4
73.2
52.6
48.9
52.2
19.0
16.7
17.7
24.8
25.4
22.8
23.6
20.3
22.4
72.2
72.0
68.3
33.0
32.2
32.8
42.0
38.2
38.7
41.7
41.0
43.0
50.9
51.1
52.8
46.4
44.4
43.7
89.8
90.4
94.9
73.2
68.0
.0
78.0
75.8
80.4
60.6
66.6
67.0
7.2
7.3
7.6
8.9
29
29
22
23
24
29
15
14
15
7
6
7
10
11
9
10
9
10
22
21
19
10
10
10
14
13
13
14
14
15
18
18
17
16
16
15
20
18
20
19
20
19
18
17
18
14
15
20
6
6
6
7
.1
.1
.4
.«
.0
.6
.4
.2
.6
.6
.8
.2
.6
.8
.2
.9
.2
.3
.2
.8
.9
.3
.0
.6
.2
.4
.0
.5
.2
.0
.0
.0
.9
.4
.0
.7
.6
.0
.3
.6
.7
.8
.3
.8
.7
.1
.2
.7
.7
.5
.5
.8
73.4
74.0
29.3
3?.0
31.0
36.6
29,4
28.5
29.2
27.6
25.9
25.9
33.6
33.5
31.3
29.7
24.7
29.5
77.7
76.1
72.6
34.0
34.3
31. $
38.9
35.2
35.9
40.8
40.0
44.9
33.8
34.5
33.9
35.4
36.0
34.2
53.0
50.2
43.5
54.0
53.3
54.0
36.0
35.5
37.2
37.2
40.0
.JO
15.6
16.0
16.5
20.1
6
6
6
.8
.7
.3
6.3
6
-999
-999
-J99
-999
9
11
9
15
16
15
8
8
7
16
15
18
8
7
9
7
7
10
8
8
8
17
18
18
9
10
12
9
9
9
6
6
9
3
3
2
7
7
7
20
17
17
18
.8
.9
.9
.9
.9
.3
.9
.2
.4
.8
.7
.9
.1
.9
.6
.5
.5
.2
.8
.3
.8
.6
.2
.7
.6
.4
.3
.3
.6
.9
.8
.6
.9
.6
.4
.9
.5
.9
.3
.3
.9
.1
.7
.8
.2
.8
.4
.2
31.6
32.5
20.6
27.0
27.2
15.0
12.5
10.7
11.9
34.7
35.0
35.1
38.1
42.3
41. 6
42. £
37.3
42.4
50.4
49.6
48.6
44.2
43.2
42.5
41.1
40.4
37.9
28.8
32.5
32.0
49.7
56.2
51.7
42.4
43.4
33.4
43.1
41.4
43.1
32.9
31.4
41.4
33.0
32.9
32.3
21.3
21.8
19.2
24.3
27.9
29.6
24.1
88
-------
SORTED DATA
U I 2
U I 2
U
U
U
u
u
u
y
u
a
u
u
u
u
u
u
u
u
u
u
u
u
u
u
u
I)
u
u
u
u
u
u
u
u
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
U 5 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
U S 2
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
1
1
1
1
1
5
5
5
5
5
5
25
25
25
25
25
25
50
50
50
50
50
50
95
95
85
85
85
85
125
125
125
125
125
125
0
0
0
1
1
1
1
1
1
5
5
5
5
5
5
25
25
1
2
2
2
2
1
1
1
2
2
2
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
2
2
3
3
2
2
2
2
2
2
3
2
2
2
2
1
2
.9
.0
.0
.0
.1
.8
.7
.8
.1
.1
.2
.4
.5
.4
.7
.6
.7
.2
.2
.2
.1
.2
.2
.3
.4
.4
.2
.2
.2
.3
.4
.4
.1
.1
.9
.8
.9
.1
.1
.9
.7
.8
.7
.8
.4
.3
.7
.7
.4
.9
.9
.6
20
8.9
9.0
8.2
8.0
7.8
17.3
18.9
19.9
14.8
14.9
15.0
35.0
39.4
40.6
22.5
15.4
14.3
13.9
13.3
14.5
15.8
15.4
14.4
71.2
70.3
70.8
27.0
28.8
29.5
26.6
29.6
28.6
17.6
17.7
17.6
5.2
5.7
4.2
5.2
5.6
5.6
5.5
5.3
4.9
5.3
4.8
4.9
5.4
4.5
5.3
7.3
7.5
7.5
7.3
7.4
7.2
7.1
10.9
11.6
11.5
9.1
9.4
9.4
14.5
14.7
16.0
10.7
1 -I .3
11.6
11.5
10.7
10.8
11.8
11.6
11.7
15.6
15.6
15.2
9.5
11.0
11.0
20.4
21.1
2Q. 9
9.7
9.3
10.5
6.0
6.0
4.8
5.3
5.1
5.8
5.8
5,6
5.1
5.9
5.8
6.0
5.6
5.0
4.9
5.1
5.3
20,
20,
19,
16,
18,
40,
38
38,
30,
32,
32,
57,
54,
64,
28,
29,
29,
17,
15,
16
19,
20,
18,
48,
46,
47,
20,
21.2
23.2
31.6
36
35
31
29
30
12
13
16
13.9
15.4
14,
15,
14
14,
13
14,
12,
14.0
14.0
13.7
6.1
9.3
,0
.2
,6
,3
,7
,5
,8
.2
,9
,0
,4
,5
,&
.2
.9
.6
.5
.1
.6
.0
.4
.2
.7
.6
.9
.0
.4
,7
,3
,6
,8
,7
.5
,9
.5
.3
,2
.6
,7
.4
,0
,7
21.8
20.2
18.7
17.4
16.9
18.4
17.9
18.2
13.5
15.0
11.8
25.4
26.9
26.8
22.5
23.1
20.3
24.5
23.4
22.5
23.7
23.0
24.3
19.3
20.9
20.7
13.7
14.7
15.8
44.0
49.7
47.0
20.7
24.8
23.8
30.8
31.7
33.0
29.7
31.7
30.3
30.4
28.2
26.7
26.8
28.9
29.6
30.8
29.8
29.2
47.6
48.0
22.0
27.2
26.6
26.1
27.5
39.5
44.9
45.2
34.0
36.2
35.7
41.3
39.4
43.5
30.3
31 .7
31.6
24.5
30.7
26.9
35.1
32.1
28.1
42.5
42.6
42.5
41.0
42.2
42.2
50.2
56.3
57.6
39.4
33.4
35.3
4.3
4.4
4.4
5.2
5.0
5.0
5.0
4.6
4.2
19.4
20.0
20.5
15.4
15.4
16.0
9.0
3.8
89
-------
SDRTED
U S
U 5
0 5
U S
U 5
U S
U S
U S
0 S
U S
U S
U S
U S
U S
U S
U S
U s
U 5
U S
0 S
U 5
U S
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
0
0
0
1
1
1
25
25
25
25
50
50
50
50
50
50
85
85
85
85
85
85
125
125
125
125
125
125
1
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
DATA
.9
.9
.1
.1
.6
.9
.5
.8
.7
.6
.9
.7
.7
.7
.9
.6
.6
.4
.5
.5
.7
.8
21
8.0
7.6
7.1
7.0
5.1
4.7
5.9
5.8
6.1
4.9
8.6
8.2
7.6
9.8
9.6
8.7
6.2
7.7
6.5
6.4
6.3
4.7
5.2
5.4
4.9
5.3
6.8
6.7
7.0
6.9
7.8
7.5
5.C
4.8
4.5
5.4
5.7
5.0
6.0
7.0
7.0
6.1
6.8
6.C
8.2
10.9
9.5
8.9
9.8
11.8
10.2
11.5
10.'
10.7
13.9
13.9
14.2
14.6
14. fc
14.4
16.5
13.8
14.9
15.7
17.7
16.7
49
48
42
49
38
40
40
39
44
35
45
43
40
43
47
40
39
41
38
39
39
32
.3
.6
.3
.4
.8
.6
.1
.5
.1
.1
.2
.1
.8
.4
.8
.8
.8
.5
.7
.3
.3
.5
9.8
9.3
9.4
9.3
11. 8
13.4
15.1
14.0
14.1
17.1
13.8
11.0
13.6
8.4
15.0
7.7
13.9
15.2
14.2
11.9
13.0
9.3
90
-------
APPENDIX B
Results for net uptake for Experiments I and II
A Students t test (P<0.05) was calculated for the null
hypothesis: Ho: y. = y_ where y. is experimental and y~ is
control for each metal for each combination of Experiment I or II,
at each sample time, with n-2 degrees of freedom shown (Deg).
Also shown are the values of E mean- C mean), the individual
means and standard deviations.
91
-------
UP
CLAH
CADMIUM
CHROMIUM
COPPf-R
NICKLE
LEAD
7INC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEA.D
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAB
ZINC
DAY
T*
T»
T«
T*
T =
DAY
T*
j =
T*
T*
T*
DAY
T*
Js
T*
T»
DAY
T*
T*
T*
T»
T»
DAY
T*
T*
T*
T«
T*
T*
DAY
T'
T*
T.
T*
T«
T*
INTERNAL EXPERIMENT N0=l
NUMBER
2.66
11.14
1 .96
1.95
1 .41
NUMBER
1.15
5.25
.77
.77
6.43
NUMBER
1.00
11.45
6.10
1.18
NUMBER
1.00
1.65
.10
1.00
1 1.00
NUMBER
.82
1.06
.52
2.37
.45
1.78
NUMBER
2.35
.00
.00
.57
.26
.74
0
NOT SIGNIFICANT
EMEAN'CKEAN
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
1
NOT SIGNIFICANT
EMEAN*CKIEAN
SIGNIF ICANF
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
5
MOT SIGNIFICANT
EMEAN'CMEAN
SIGNIF ICANT
£MEAN=CMEAN
SIGNIFICANT
NOT SIGNIFICANT
25
NOT SIGNIFICANT
EMEAN=CMEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICAuT
50
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
125
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NDT SIGNIFICANT
NOT SIGNIFICANT
EMEAN-CMEAN'
EME AN-CH6 AN •
EMEAN-CMEAN*
EHEAN-CMEAN*
EMEAN-CME AH =
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CME AN =
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAM-CMfc«N=
EMEAN-CME AN =
EHEAN-CMEAN*
EMEAN-CMEAN=
EMEAN-CMEAN*
tMEAN-CMEAN=
EMEAN-CMEAN*
EHEAN-CMEAN=
EMEAN-CME AN*
EMEAN-CME AN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EME-AN-CME AN*-
EMEAN-CME AN*
EMEAN-CMEAN*
EHEAN-CHEAN*
EMEAN-CMEAN*
EHEAN-CME AN*
EMEAN-CMEAN*
EMEAN-CMEAN*
1.54
14.57
-5.04
-3.66
-36.15
-1.77
-15.70
2.35
2.08
-115.10
-1.77
-9.55
11.90
15.40
.93
-10.25
-.65
-3.17
92.40
-1.00
1.4,3
1.38
-4.33
-1 .13
-86.67
-.32
-.00
-.00
-.95
.17
9.77
DEG =
DEC*
OEG*
OEG =
DEG =
DEG*
DEC*
DEG =
DEG =
DEG =
DEG*
DEG =
DEG =
DEG =
DEG =
OEG*
DEo*
OEG*
BEG*
DEG*
DEG =
DEo*
LiEG*
OEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
4 EMEAN*
EMEAN*
1 EMEAN*
4 EMEAN*
4 EMEAN=
4 EMEAN*
3 EMEAN*
EHEAN=
j £MEAN=
3 EMEAN=
3 EMEAN*
J EMEAN*
2 EMEAN=
EMEAN*
2 EMEAi\ =
EMEAN*
2 EMEAN*
2 EMEAN*
2 EMEAN*
EMEAN*
2 EMEAN=
2 EMEAN*
2 EMEAN*
2 EMEAN*
4 EMEAN*
4 EMEAN*
H EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
7 EMEAN'
7 EMEAN*
7 EMEAN*
7 EMEAN*
7 EMEAN*
7 EMEAN=
2.55
1.70
31.50
.45
.25
82.00
.40
1.70
6.35
2.80
2.33
173. 4C
. 15
1.70
10.55
.45
12.15
229.10
2.00
1.70
11.05
4.77
.25
185.90
2.37
3.73
7.88
7.33
8.70
183.13
1.46
1.70
4.45
4.68
5.44
116.50
EDEV*
EOEV *
£&£•¥•
EDEV*
EDEV*
EDEV =
EDEV*
EDEV-
EDEV*
EDEV =
EDEV'
tOEV*
fcDEV*
EDEV =
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EO£V =
EDEV*
EDEV*
ED€V=
EDEV*
EDEV =
EDEV*
EDEV*
E&EV-
EDEV*
E-OEV =
EDEV =
EDEV-
EO£V-=-
EDEV«
EOEV*
.21 CHEAN*
.2.1 CMEAN*
.14 tflEAft"
.00 CMEAN*
.00 CMEAN=
1.41 CH£AN*
.43 CMEAN*
.43 CHEAN*
3.39 CrtEAN*
4.07 CMEAN=
3.61 CMEAN*
15.42 CMEAN'
.00 CHEAN*
.00 CMEAN*
.92 CMEAN*
.92 CHEAN*
2.76 CMEAN*
18.10 CMEAN'
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1.77 CMEAN*
6.12 CMEAN*
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1.15 CMEAN*
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2.96 CHEAN'
2.21 CHEAN*
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13.30 CHEAN*
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14.78 CHEAN'
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1.01 CDEV*
1.70CDEV*
16.92 CDEV'
5.49 CDEV*
3.91 CDEV
118.15 CDEV*
2.17 CDEV'
1.70COEV'
24.05 CDFV*
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288.50 CDEV*
1.92 CDFV«
1.70CDEV'
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213.70 CDEV*
1.07 CDEV*
1.70CDEV*
21.30 CDEV*
5.42 CDEV*
3.42 CDEV'
93.50 CDEV'
3.37 CDEV*
2.30 CDEV*
6.50 CDEV'
11.67 CDEV*
9.83 CDEV'
269.80 CDEV*
1.77 CDEV*
1.70 CDEV'
4.45 CDEV'
5.62 CDEV'
5.27 CDEV*
106.72 CDEV*
.76
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1.74
3.40
2.51
34.09
2.66
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3.04
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26.02
2.51
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3.25
1.31
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8.63
7.04
4.49
11.74
1.76
1.04
3.55
2.27
3.38
83.06
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2.75
1.11
24.66
-------
CLAN
SHELL
EXPERIMENT N0=l
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CADMIUM
CHROMIUM
COWER
NICKLE
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ZINC
CADMIUM
CHROMIUM
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T« .48
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T= .11
T* 1.26
DAY NUMBER
1* .88
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T* .39
T» .43
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T* .33
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T* .00
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T= .92
T= .56
0
NOT SIGNIFICANT
NOT SIGNIFICANT
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NUT SIGNIFICANT
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CADMIUM
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3.94
1.57
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1.03
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1.15
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13.62
2.99
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3.05
5.33
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3.14
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1.46
1.63
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1.06
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2.65
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1.12
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SIGNIFICANT
SIGNIFICANT
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EMEAN*CMEAN
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SIGNIFICANT
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EMEAN-CMEAN=
EMEAN-CME AN=
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1.45
1.70
4.45
11.95
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18.05
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10.40
7.05
10.65
1.90
1.70
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10.43
11.97
12.62
1.45
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4.45
11.95
7.60
9.95
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3.04
-------
HOLOTHUR INTERN
CADMIUM
CHROMIUM
C-WPER
NICKLE
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ZINC
CADMIUM
CHROMIUM
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1.75
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2.62
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3.33
22.17
4.17
NUMBER
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5.91
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1.72
15.47
16.31
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22.33
9.03
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67.37
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SI
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125
NOT
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EXPERIMENT N0=l
SIGNIFICANT
SIGMF I CANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
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1.15
7.50
7.27
16.50
4.15
32.10
2.10
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14.53
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63.20
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37.35
22.45
79.30
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26.80
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-------
HOLOTHUR MUSCLE EXPERIMENT N0=l
CADMIUM
CHROMIUM
COPP-E*
NKKLE
LEAD
ZINC
CADMIUM
CHROMIUM
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DAY NUMBER 1
T« .48 NOT SIGNIFICANT EMEAN-CMEAN'
T« 1.97 NOT SIGNIFICANT EME AiM-CME AN =
T» .00 NOT SIGNIFICANT EME AN-CME AN •
T= 2.46 SIGNIFICANT EME AN-CMt AN =
T' .15 NOT SIGNIFICANT EME A ''-CME AN =
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DAY NUMBER 5
T' .87 NOT SIGNIFICANT EMEAN-CrtEAN*
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T» .47 NUT SIGNIFICANT EME Ai»-CME AN =
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DAY NUMBER 25
T» 1.00 NOT SIGNIFICANT EMEAN-CMEAN=
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T» .48 NOT SIGNIFICANT EMEAN-CKEAN*
T« 1.48 NOT SIGNIFICANT EMEAN-CMEAN'
DAY NUMBER 50
NO EXPERMENT MEAN" 1.65 DEVIATION*
NO EXPERMENT M£AN = 6.35 OEVlATILiN'
NO EXPERMENT MEAN= 4.45 DEVIATION*
NQ EXPEkMENT MEAN* 12.22 DEVIATION*
NO EXPERMENT MEAN* 11.15 DEVIATION*
NO EXPERMENT MEAN* 41.87 DEVIATION
DAY NUMBER 125
T= 1.24 NOT SIGNIFICANT EMEAN-CMEAN*
T= .81 NOT SIGNIFICANT EHEArt-CMEAN*
T« .00 NOT SIGNIFICANT EMEAN-CMEAN*
T» 1.85 NOT SIGNIFICANT EMEAN-CMEAN'
T« 2.11 NOT SIGNIFICANT EMEAN-CHt AN*
T« .48 NOT SIGNIFICANT EME AN-CrtE AN =
-.as DEG*
-.98 DEG =
-.00 DEG =
-2.20 DEG*
.44 DtU*
15.77 DEG*
1.01 DEG*
.90 DEG*
-.00 DEG*
1.82 UEG*
-2.15 bEG*
16.97 DEG*
-.77 DEG*
-.65 OEG=
3.35 OEG =
.75 NUM' 4
^.24 NUM* 4
.00 HUM* 4
3.37 NUM* 4
2.76 NUM* 4
9.92 NUrt* 4
-1.18 uEG*
-1.47 DEG=
.00 DEG*
-6.40 DEG =
-8.77 DEG=
3.60 DEG*
7
7
7
6
7
6
3
4
4
f,
4
4
2
2
2
4
4
4
4
4
4
EMEAN*
EMEAN=
EMEAN*
EMEAN=
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN=
EMEAN*
fcMEAN*
EMEAii =
C M b A N =
EMEAN*
EMEAN*
EMEAN=
EMfcA(4 =
EMEAN*
EMEAN=
EMEAN*
EMEAN*
EMEAN*
1
1
4
8
8
71
1
2
4
10
7
62
1
4
3
12
67
1
3
4
9
g
49
.19
.70
.45
.10
.74
.20
.78
.60
.45
.37
.55
.52
.15
.70
.45
.85
.90
.70
.30
.^fr
.45
.95
.90
.75
EDEV =
EDEV*
•ffrtV —
EDEV*
EDEV*
EDEV"
EDEV =
EDEV-
EDEV*
EDEV*
EDEV*
EDEV*
EDEV-
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV*
EO£V-=
EDEV*
EDEV»
£&£^V>
EDEV*
.68
.00
^0
.84
4.08
19.62
1.43
1.04
.00
1.67
6.09
10.72
.OO
.00
.00
,00
1.84
.28
''
.14
»A2
.00
.07
.00
2.76
CMEAN*
CMEAN'
one AN-
CMEAN*
CMEAN*
CMEAN*
CMEAN*
CMEAN-
CMEAN*
CMEAN*
CMEAN*
CMEAN*
CMEAN'
CMEAN*
CMEAN*
CMEAN*
CMEAN'
CHEAN=
CMEAN*
C-M£AN =
CMEAN'
CMEAN*
C*EAN«
CMEAN*
5
1.44 CDEV'
2.67 CDEV'
4.45 COEV-
10.30 CDEV'
8.30 CDEV*
55.42 COEV«
.77 CDEV'
1.70 CDEV'
4.45 CDEV'
8.55 CDEV'
9.70 CDEV'
45.55 CDEV'
.92 CDEV'
1.70CDEV'
4.45CDEV
3.65CDEV'
13.55 CDEV'
64.35 CDEV'
2.47 CDEV'
5.17 CDEV'
4.45 CDEV«
16.35 CDEV'
11.67 CDEV'
45.95 CDEV'
.88
1.13
.00
1.58
4.59
10.66
.88
.00
.00
.78
.28
2.76
1.10
.00
.00
.07
.49
3.18
1.27
2.41
.00
4.61
5.55
10.38
-------
MANGROVE
DAY
CADMIUM T«
CHROMIUM T»
COPPER
NICKLE T«
LEAD
me T«
DAY
CADMIUM T«
CflR-QMIUM
COPPER
NICKLE T«
LEAD
ZINC
DAY
-------
MANGROVE STEM
DAY NUMBER 0
EXPERIMENT NO*!
00
CADMIUM T*
CHROMIUM T*
tOPPEB T-*
NICKLE T*
LEAD
ZINC T*
DAY
CADMIUM T*
C-HROHIUM T*
COPPER
MCKLE T*
LEAD T*
ZINC T*
DAY
.77
28.17
.00
.81
•4-9
NUMBER
.00
.00
.00
.00
.00
NUMBER
CADMUW T* 45404.67
CHROMIUM
COPPER
MCKLE T =
LEAD T*
ZINC T*
DAY
CADMIUM T*
CHROMIUM
COPPER
NICKLE T*
LEAD T*
11 NC T*
4.92
.00
2.91
NUMBER
1.00
3.46
.45
1.92
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
EKEAN*CKEAN
NOT SIGNIFICANT
5
NOT SIGNIFICANT
NOT SIGNIflCANT-
EMEAN=CMEAN
NOT SIGNIFICANT
NUT SIGNIFICANT
NOT SIGNIFICANT
50
SIGNIFICANT
EME-AN=ChEAU
Ei-l£AN=CME AN
SIGMF ICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
125
NOT SIGNIFICANT
EMEAN =CKEAN
EHc Ait =C(iE AN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN-CMEAN*
EriEAN-CMEAN*
fcH€ Aff—C HE AN •
EMEAN-CMEAN*
EME A.V^CME AW*
EMEAN-CMEAN =
ENE-Ali-CME AN*
EMEAN-CME AN =
E.HE AN-CME 4N =
EMEAN-CMEAN=
Ert€ AW-=C#E AN*
EWE AU -CMEAN -
tMEAN-CMEAN*
EMEAN-CME AW*
EMEAN-CME M =
EMEA.4-CMEAN*
EMEAN-CMEAN*
EME Art-CME AI4 =
-.03 DtG*
2.10 DEG=
.00 1>EG-
-.20 DEG*
.32 DEG*
.20 DEG*
-3.10 DEG*
-7.20 DEG=
-15.75 D£G=
-27.80 DEo=
-.60 DEG*
.55 DEG*
3.75 DEG=
-2.80 DEG=
-.05 DEG=
1.35 DEG=
.50 DEG=
1.45 DEG=
3 EMEAN*
3 EMEAN*
3 EMEAN*
3 EMEAN=
EMEAN.*
3 EMEAN*
0 EMEAN-
0 EMEAN*
EMEAN=
0 EMEAN*
0 EMEAIi*
0 EMEAN*
2 EMEAN*
cMEAN =
EMEAN*
2 EMEAN*
2 EHEAU*
2 EMEAN*
2 EMEAN=
EMEAN*
EMEAN*
2 EMEAU*
2 EMEAN*
2 EMEAN*
.30
3.80
4-r45
2.00
.25
5.95
1.20
1.70
4.45
2.40
.25
3.80
.80
i.70
4.45
2.25
4.00
2.95
1.00
1.70
4.45
3.70
4.00
5.65
EDEV*
EDEV*
EDEV'
EDEV*
EOEV*
EDEV*
tDEV*
EDEV =
EDEV*
EDEV*
£OEV =
EDEV*
EDEV*
EDEV*
EDEV*
fcOEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV =
EOEV*
EDEV*
EOEV=
.00
.14
.60-
.28
.28
.35
.00
.00
.00
.00
.00
.00
.00
.00
.00
.07
.00
.21
.00
' .00
.00
.42
1.41
1.04
CMEAN*
CMEAN*
CHEAN*
CMEAN*
CrtEAN*
-CMEAN*
CMEAN*
CHEAN*
CMEAN*
CMEAN*
CMEAN*
CMEAN*
CHEAN*
CMEAN*
CMEAN*
CMEAN*
CMEAN*
CrtEAN*
CHEAN*
CKEAN*
CMEAN*
CttEAN*
CMEAN*
CrtEAN*
.33 COEV*
1.70 COEV*
4.45 COEV-
2.20 COEV*
.25COEV*
5.63 CDEV*
1.00 COEV*
4.80 COEV*
4.45CDEV*
9.60 CDEV*
16.00 COEV*
31.60 COEV*
1.40 CDEV*
1.70CDEV*
4.45COEV*
1.70 CDEV*
.25 CDEV*
5.75 CDEV*
1.05 CDEV*
1.70CDEV*
4.45CDEV*
2.35 COEV*
3.50 COEV*
4.20 CDEV*
.04
.00
.00
.26
.00
1.40
.00
.00
.00
.00
.00
.00
.00
.00
.00
.14
.00
1.34
.07
.00
.00
.35
.71
.14
-------
MANGROVE LE
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
MCKLE
LEAO
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAO
ZINC
DAY
T«
f.
T*
T*
DAY
T*
T*
T»
T*
DAY
T*
T-
T*
NUMBER
4.62
.00
.00
12.89
NUMBER
1.41
8.0*
2.83
10.11
NUM6ER
2.83
3.00
9.47
EXPERIMENT NQ*1
5
NOT SIGNIFICANT
EMEAN'CHEAN
NOT SIGNIFICANT-
NOT SIGNIFICANT
EMEAN =>CMEAN
SIGNIF1CAN1
50
NUT SIGNIFICANT
EHEAN-CHEAN*
SIGNIF ICAIiT
NOT SIGNIFICANT
SIGNIFICANT
125
NUT SIGNIFICANT
EKEAN'CMEAN
EMEAN=CKtAN
E.1EAN*CKEAN
NOT SIGNIFICANT
SIGMF ICANT
-.80 DEG = 1 EMEAN*
EMEAN*
-4.55 OE6* 1 EMEAN*
EMEAN-CHE
AN =
tHEAN-CHEAN*
EHEAN-CHE
EMEAN-CME
E4EAH-CME
EMEAN-CME
EMEAN-CME
EM£A,>i-CME
AN =
AN =
AN*
AN"
ANS
AN >
-4
-3
-
_£,
-2
-3
-
1
2
.50
.35
.10
.05
.00
.95
.20
.50
.55
OtG*
DEG =
OEG =
I>EG =
DEG =
UtG*
-1
1
2
2
i
2
2
2
2
EMEAN*
EMEftH^
EMEAN=
EMEAN=
EMEAN=
||:
1
4
6
1
1
it
3
4
7
1
4
5
10
•
*
m
.
*
•
*
*
*
^
,
•
B
B
.
.
.
.
70
70
45
00
25
IS
05
70
45
45
50
80
95
70
45
00
50
25
EDEV
EOE
EOE-V
EOEV
«
V =
*
9.
EDEV*
EOEV
EDEV
a
•
fcDEV*
tOE
cOtV
EOEV
EDEV
EOEV
tot
v =
=
X
•s
3
V -
fcOEV
hJE
EDEV
tDEV
V =
a
s
.14
.14
.
CMEAN*
CrtEAN*
CHEAN'
CHEAN*
CHEAN*
CM£ AN*
CHEAN*
CMEAN-
CHEAN*
CHEAN*
CHEAN*
CrtEAN*
CMEAN'
CHEAN*
CMEAN*
1
1
9
4
11
1
1
4
7
6
11
1
1
4
4
7
*
•
•
•
•
•
•
*
*
•
*
•
•
•
•
•
•
•
50 CDEV
70CDEV
00 CDEV
50 CDEV
25CDEV*
50 CDEV*
15 CDEV
70CD6V*
45CDEV*
SO CDEV*
50 CDEV*
75 CDEV*
15 CDEV*
70COEV*
45COEV*
OOCOEV
00 CDEV*
70 CDEV
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.07
.71
.71
.35
.07
.00
.14
-------
MANGROVE
CADHIUH
CHROHIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
M CHROMIUM
§ COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY
T«
T«
T>
T«
DAY
T«
T«
T»
T'
DAY
T*
T'
T-
T»
T'
T«
ROOT
NUM3ER
3.00 N
.00 N
3.00 N
3.77 N
NUMBER
3.00 N
****
2.83 N
12.63
NUMBER 1
.28 M
3.02 N
.00 N
5.28
3.06 tf
4.33
EXPERIMENT N0*l
NOT SIGNIFICANT
EMEAN=CK,EAN
EMEAN'CHEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
50
MOT SIGNIFICANT
EMEAN-CMEAN
EKEAM'CHEAN
SIGNIFICANT
NOT SIGNIFICANT
SIOH1F1CANT
25
MOT SIGNIFICANT
NOT SIGNIFICANT
NLT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
fuc4;i-CMfc AN*
ENEAN-CMEAN*
£MEAN-CHE6N =
EHEA.1-CMEAN =
EHEAM-CMtAN=
EKE AN-CME AN =
ENEAJa-CME AN -
EMEAN-CHEAN=
EME AM-CM.E Ai«-
EME AN-CHt 4N=
ErtEAiJ-CM£AN =
E,l£Ai4-CMEAN =
EMEAN-CMEAN'
tMEAN-CMEAN*
-6
-1
3
2
12
..
3
5
3
19
.15
.00
.50
.70
.30
.00
.65
.12
.77
.00
.75
.00
.05
DEO'
l)EG =
DEG =
DE&=
D£G =
DEG =
OEG=
OEG =
DEG =
DEG =
UfcG =
DEG =
DEG =
DE& =
2
0
2
2
2
1
2
2
3
3
3
3
3
3
EMEAN=
EHEAN=
EHEAN'
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
£MEAN=
EMEAN=
EM£AN»
EMEAN=
£MtAN=
EHEAN =
ENEAN=
EHEAN=
EMEAN=
1.20
1.70
4.45
.00
4.00
11.55
1.80
1 . 7ft
4.45
9.00
7.50
23. 9S
1.05
7.00
4.45
16.45
10.50
49.35
EO£V«
EDEV =
§&€V*
EDEV*
EDEV =
EOEV =
EDEV*
EOEV>
EOEV =
EDEV*
£DEV =
EDEV =
EOEAA*
fcDEV =
EOEV*
cDE V-^
EDEV« '
cDEV«
.00
.00
^e
.00
.00
V.34-
.00
.00
.00
.00
.71
.07
,7»
1.41
.00
2^0-5
.71
6.29
CHEAN'
CMEAN'
€^€AH«
CHEAN'
CMEAN'
— €M£- AM *
fMEAN'
CMEAN'
CMEAN'
CMEAN'
CKEAN*
CHEAN'
CMEAN-
CMEAN'
CMEAN=
CMEAN'
CMEAN'
CMEAN'
1.05 COEV
1.70CDE V
4.45CDEV
6.00 C06V
5.50 CDEV«
7.85 COEV-
l.$0 CDEV*
1.70COEV-
4.45COEV'
5.50 CDEV*
5.50 CDfcV
11.30 CDEV*
1.17 COEV-
3.23 COEV*
4.45 CDEV'
10.70 CDEV'
7. SO COEV*
50.30 CDEV.
.07
.00
»
.00
.71
.35
.1*
.00
.00
0.0
.71
1.41
.06
1.34
.00
.17
1.22
3.87
-------
NERITA INTERNAL EXPERIMENT NO =1
CADMIUM
CHROMIUM
COWER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COWER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
OiROKlUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICK4.E
LEAD
ZINC
DAY NUMBER 0
T« .00 NOT SIGNIFICANT EHE AiM-CME AN =
T« .00 NOT SIGNIFICANT EME Art -C ME AN =
T» .00 NOT SIGNIFICANT EMEAH-CMEAN-
T« .00 NOT SIGNIFICANT EME Art-CME AN =
T« .00 NOT SIGNIFICANT EMEAN-CMEAN=
T« .00 NOT SIGNIFICANT EMEAN-CMEAN'
DAV NUMBER 1
NO EXPERHENT HEAN= .15 DEVIATION*
NO EXPERHENT MEAN= 1.70 OEV1AT!UN=
NO EXPERHENT MEAN = 62.20 DEVIATION
NO EXPERMENT KEAN = .45 DEVIATION =
NO EXPERKENT HEAN- 23. JO DE V I AT Ilifc =
NO EXPERMENT MEAN= 173.60 OEV1ATIUK=
DAY NUMBER 5
T« .00 NOT SIGNIFICANT EMEAN-CMEAN=
EMEAN=CUEAN
T« .00 NUT SIGNIFICANT tME Arf-CME «N =
T« .00 NOT SIGNIFICANT EMEAN-CMEAN=
T« .00 NCT SIGNIFICANT EMEAN-CMEAN=
T« .00 NOT SIGNIFICANT EMEAN-CMEAN=
DAY NUMBER 25
EMEAN=CMEAN
T« .00 NOT SIGNIFICANT EME Af.-CME AN =
T« .00 NUT SIGNIFICANT EHEAN-CMEAN-
EMtAN=CMEAU
T- .00 NCT SIGNIFICANT EMEAN-CMEAN=
T> .00 NGT SIGNIFICANT tME AN-CMfc 6N =
DAY NUMBER SO
T» .00 NOT SIGNIFICANT EME AN-CMfc AN =
T» .00 NOT SIGNIFICANT tMEAN-CMEAN-
f« .00 NOT SIGNIFICANT EME Art-CME AN =
T» .00 NOT SIGNIFICANT EME AN-CME AN =
T« .00 NOT SIGNIFICANT EMEAN-CMEAN=
T« .00 NOT S1GNIF1CANI EMEAN-CMEAN=
DAY NUMBER 125
NCI EXPERMENT MEAN- 3.00 DEV1AT-1UW-
NO EXPERMENT MEAN' 41.60 DEVIATION*
NU EXPERMENT MEAN' 21.40 DEVIATION'
NO EXPERMENT MEAN= 9.90 DEVIATION'
NU EXPERMENT MEAN" 3.80 DEVIATION'
NO EXPERHENT MEAN* 62.30 DEVIATION-
1.90 OfcG=
7.30 DEG=
-ft. 30 DEG=
-.tO DEG=
20.10 DEG=
-9.60 DEG=
.00 NUM= 1
.00 NUM= 1
.00 NUM= 1
.00 NUM- 1
.00 NUM= 1
.00 NUM= 1
2.95 DEG=
8.10 DEG=
23.55 UEG=
-10.60 OEG=
-11.60 DEG-
-5.70 DEG=
6.40 OEG=
-.20 DEG=
-70.60 UEG=
.90 OEG=
3.90 UEG=
J.10 DEG=
-.90 OEG=
-10.50 OEG=
26.70 DEG=
.00 NUH- 1
.00 NUH= 1
.00 MUH= 1
.00 HUM- 1
.00 NUH= 1
.00 NUM= 1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
EMEAN=
EMEAN*
EMEAN=
EMEAN=
EMEAN=
EHEAN-
EMEAN=
ErtEAiM =
EMEAN=
EMEAN=
EMEAN=
EHEAN=
fcMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
tMEAN=
EMEAN=
EMEAN»
EMEAN"
EMEAN=
EMEAM=
5
10
46
27
40
119
3
1
58
24
18
157
1
56
34
118
4
5
53
16
6
168
.40
.80
.90
.00
.90
.70
.10
.70
.40
.00
.10
.00
.15
.70
.00
.45
.20
.50
. 10
.60
.90
.40
.20
.50
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV-
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EOEV =
EDEV«
EDEV =
EDEV =
EDEV =
.00 CMEAN=
.00 CHEAN=
.00 CM£AN=
.00 CMEAN=
.00 CMEAN=
.00 CHEAN=
.00 CMEAN»
.00 ChEAN*
.00 CMEAN'
.00 CMEAN*
.00 CMEAN=
.00 CMEAN=
.00 CHEAN=
.00 CMEAN-
.00 CHEAN*
.00 CHEAN'
.00 CMEAN»
.00 CMEAN=
.00 6HEAN'
.00 CMEAN=
.00 CMEAN'
.00 CMEAN=
.00 CMEAN"
.00 CMEAN<
10
3.50
1.50
55.20
27.40
20.80
129.30
.15
COEV«
CDEV =
CDEV«
CDEV«
CDtV«
CDEV«
CDtV-
1.70COEV=
50.30
.45
28.70
\68.60
CDEV =
CDbV«
CDEV =
CDEV«
.15CDEV«
7.40
49.60
CDEV-
CDEV«
.45CDEV-
34.40
189.10
a. 20
1.70
50.70
17.30
16.70
141.80
CDEV-
CDEV-
CDfcV-
CDEV-
CDEV-
CUtV
CDEV«
COEV-
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
-------
to
NERITA SHELL EXPERIMENT N0"l
CADMIUM
CHROMIUM
CCPPER
NICKLE
LEAD
Z4WC
C.ADM IUM
CMRUNIUH
COPPER
MCKLE
L-EAD
ZINC
CAOK1U*
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
L£AO
2-INC
CADMIUM
CHRO.MUJM
COPPER
NICKLE
•tiAO
ZINC
CAOiilUK
CHROMIUM
COPPER
V1CK-U
LEAD
ZINC
DAY NUMBER 0
T« 1.15 NOT SIGNIFICANT EMEAN-CMEAN"
T« 1.15 NOT SIGNIFICANT EKE AN-CME AN"
T- .00 NOT SIGNIFICANT EMEAK-C-ME AN «
T- 1.15 NOT SIGNIFICANT EME AN-CME £N *
T" .91 NOT SIGNIFICANT £ME A,*-CHE 4N *
T« .72 NOT SIGNIFICANT EME AW-ChE M -
DAY NUMBER 1
EM£AN=CM£AN
EMEAN=CMEAN
EME AN =CHE AN
EHEAi«=CHE«N
T* 5.21 SIGNIFICANT EME AiM-CMt AN »
T« 4.67 SIGNIFICANT tMEAN-CMEAU"
DAY NUMBER 5
T" 1.00 NOT SIGNIFICANT EMi AN-CME AN *
T» 1.00 NOT SIGNIFICANT EME AN-CME AN "
EMEAN =CME Ail
EMEAN=CMEAN
T« .41 NOT SIGNIFICANT EMEAN-CMEAN*
T» 1.02 NOT SIGNIFICANT EMEAN-CMEAN"
DAY NUMBER 25
EMEAN=CMfcAN
EM£AN=O;EAN
EMEAni=CKEAN
EMEAN "CMEAN
T« 1.33 NOT SIGNIFICANT EME AN-CME AN "
T" 1.34 NOT SIGNIFICANT EME Afc-CME AN =
DAY NUMBER 50
T« .42 NOT SIGNIFICANT EME AN-CMc AN «
T» .26 NOT SIGNIFICANT EME A«-CH£ AN -
T» .00 NOT SIGNIFICANT EME A.1-CME AN =
T" .45 NOT SIGNIFICANT EMEAN-CMEAN"
T" .23- NOT SIGNIFICANT EMEAn-CHEAN"
T= .52 NOT S1GN1FJ-CANT EMEAN-CHEAN"
DAY NUMBER 125
NO EXPERHENT HEAN" 4.35 DEVIATION"
NO EXPERMENT MEAN" 3.43 DEVIATION"
NO EXPERHENT MEAN" 4.45 DEVIATION"
NO EXPERHENT MEAN* 33.JO-OE-V-I AT4UN*
NO EXPERHENT MEAN" 42.4.5 DEVIATION"
m EXPERHENT HEAN* 7.00 DEVIATION"
2.72 DEG"
2.47 OEG"
-.00 OEG"
17.17 DEG"
-12.38 UEG*
-.90 UEG=
14.35 JEG"
-2.85 OtG"
-1.67 i)EG =
l.SO DEC"
-6.10 DEG=
-1.05 OEG=
3.25 UEG"
-1.90 DEo=
-.23 UEG"
-.55 ufcG=
.00 UEG"
.20 DEG"
-.77 OEG"
-.37 OEG"
.07 I«UM" 2
.07 NUM= 2
.00 NUM* 2
.00 NUH" 2
1 .91 NUH" 2
.28 NUM* 2
4
4
4
4
if
4
2
2
2
2
2
2
2
2
4
4
4
4
4
4
EMEAN"
EMEAN"
EMEANc
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EM£AN=
EMEAN"
EMEAN"
EMEAN"
EMEAN "
EMEAN"
EMtAN=
EMEAN"
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EM£AN=
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tMEAN=
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17
63
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72
10
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33
43
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.62
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.45
.75
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.20
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EDEV"
EDEV"
EDEV-
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EDEV =
EOEV"
EOEV =
EDEV'
EDEV"
EDEV =
EDEV"
EDEV"
EUEV"
EOEV"
EDEV
EDEV"
3.15
2.86
.00
19.85
18.17
1.66
1.66
1.66
1.66
1.66
1.63
.71
.00
2.12
2.12
2.12
21.14
1.34
1.34
1.34
1.34
1.34
.14
1.27
.07
.28
.00
.64
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CMEAN'
CMEAN'
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CMEAN"
CMEAN"
CHEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CttEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CMEAN"
CHEAN'
CMEAN"
CHEAN"
CHE- AN"
CMEAN"
11
.15 CDEV"
1.70 CDEV
4.45 CDEV
.45 CDEV"
75.85 CDEV
7.45 COEV"
,15CDEV=
1.70CDtV
4.45COEV"
.45COEV"
59.30 CDEV"
11.95 CDEV"
1.82 CDEV"
1.70 CDEV
4.45CDE V
.45COE V
72.85 CDEV
9.00 CDEV"
.15CDEV
1.70COEV
4.45CDE V
.45CDEV
69.45 CDEV
12.50 CDEV
4.47 CDEV
4.15 CDEV
4.45 CDEV'
32.95 CDEV
43.97 CDEV"
7.47 COEV"
.00
.00
.00
.00
1.06
.07
.00
.00
.00
.00
3.54
.49
2.37
.00
.00
.00
.35
.57
.00
.00
.00
.00
3.46
1.56
.71
2.8^
.00
.47
4.52
.97
-------
OYSTER
DAY
CADMIUH
CHROMIUM
COPP6R
NICKLE
LEAD
ZIKC
CADMIUM
CHROMIUM
COPPER
-e NICKLE
2 LEAD
W ZINC
CADHIUh
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
T»
T =
T«
1 =
T»
T =
DAY
T»
T-
T =
T =
T«
T«
DAY
T«
T«
T»
T»
T«
T«
DAY
T«
T«
T»
T«
T«
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INTERNAL EXPERIIWNT NO = L
NUMBER 0
.11
.37
1.30
.4$
.19
.74
NUMBER
.00
.00
.00
.00
.00
.00
NUMBER
.00
.00
.00
.00
.00
.00
NUMBER
.00
.00
.00
.00
.00
.00
NOT
NOT
NLT
NOT
NOT
NUT
1
NOT
NOT
NUT
NOT
NOT
NUT
5
NOT
NOT
NOT
NCT
NCT
NOT
25
uCT
Ml
NCT
iiLF
NCT
NOT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFI CANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGN! PI CANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SUNIFICAM
SIGNIFICANT
SIGNIFICAfcT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
cMEAN-CMt AN*
EM£AN-CMEAN=
EMEAN-CHE AN-
E,1E AM-CME AN =
EME AN -CHE AN =
tNt Art-CMt AU =
EMEA,\-CMEAiJ =
EMEA.1-CKE AN =
EME An-CrtE AN=
tME AU-CME AN=
L'lE A.<-CME Ut =
EMEAN-CME AN*
EMEAn-CHE AN=
EMEAN-CMEAN*
E.4EA,»-tME AN =
ErtEAi'.-CMEAN =
EMEAN-CME»N=
E.lEAU-CMt AN =
EME Aii-CilE AN =
ErtEArJ-CKEAN =
cMEAN-CME AN =
EMEAi«-CMEAN =
EMEAH-CMt AN=
EMEA.'j-CME AN =
.15
-1.55
204.55
2.15
1.15
416.05
-.10
-Z.50
99.30
2.00
-1.20
140.20
1.20
2.20
-202.70
6.10
30.50
-622.40
3.00
.10
257.60
23.40
27.80
-6.^0
DEG =
DEG =
DEG*
DEG =
UEG =
DEG =
DtG =
Obb =
DEG =
UtG =
UtG =
UEG =
DEG =
UEG =
OEG=.
ObG-
OEG =
UEG =
DEG =
OEG =
L>EG =
OEG =
DEG =
DLG =
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMtAN=
0 EMEAN=
0 EMEAN=
0 EMEAM=
0 EMEAN=
0 EC,EAN =
0 EMEAN=
0 EMEAN=
0 EMEAN=
0 EMEftN=
0 EMEAN=
0 EHEAN=
0 EHEAN=
0 EMEAN=
0 EHEAN=
0 EMEAN=
0 cHEAN=
0 EMtAN=
0 ErtEAN=
2.65
3.75
573.05
11. BO
10.75
1315.40
2.50
1 .70
220.90
10.90
8.80
1057.00
4.00
3.90
87.90
13.20
36.20
527.60
6.90
4.UO
363.90
30.90
33.50
611.70
bOEV =
EDEV =
EOE-V =
tDEV*
EOEV =
EOEV =
fcOEV =
EDEV =
EDEV =
tOEV =
EDEV =
EDEV =
EOEV =
EOtV =
EOEV =
EUEV =
EDEV =
kOEV =
EDEV =
EUtV =
ED£V =
EDEV =
EDEV =
EOEV =
.07
2.90
57.77
.85
.64
392.87
.00
.00
.00
.00
,00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.uo
CHEAN=
CMEAN=
CMEAN*
CMEAN=
CfltAN^
CrtEAN=
CMEAN=
CMEAN-
CMEAN=
CMEAN=
CHEAN=
CrttAN=
CHEAN=
CMEAN=
CMEAN=
1C, tAN =
CMEAN=
C«£AN=
CMEAN=
CMEAN=
CMEAN=
ChEAN=
C.MErtN =
CHEAN=
12
2.50
5.30
368.50
9.65
9.60
899.35
2.60
4.20
121.60
8.90
10.00
916.80
2.60
1.70
290.60
7.10
5.70
1150.00
3.90
3.90
106.30
7.50
5.70
620.10
CDEV =
CDEV =
CObV"
CDEV*
COEV«
CDEV«
COEV«
CDEV-
CDEV =
CUEV =
CO*V =
CDEV =
CDEV =
CDEV-
CDEV*
CDfcV
CDEV =
CUEV =
CDEV'
CDEV =
CDEV«
CDfcV*
COfcV =
CUEV«
1.98
5.09
214.25
«.72
8.34
685.66
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.03
.00
.00
.00
.00
.00
-------
OYSTER
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC,
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY
T«
Js
T*
T*
T =
DAY
T"
T =
T =
T =
T =
DAY
T»
7 =
T*
T'
T'
T«
DAY
fr
T*
T =
T«
T >
T«
SHELL
NUMBER
31.00
.14
7.00
1.86
11.14
NUMBER
.63
3.64
.95
.45
1.57
NUMBER
.91
'.25
.00
.05
7.82
6.09
NUMBER
.67
8.22
1.05
7.70
1.28
7.41
0
SIl
NOT
El
SIl
NOT
Sli
1
NOT
NOT
El
NOT
NOT
NGT
5
NOT
NOT
NOT
NOT
SH
SIl
25
NOT
NOT
NOT
SIl
NOT
sn
EXPERIMENT NO'l
13
SIGNIFICANT
SIGNIFICANT
EAN'CKEAN
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
EMEAN=CMEAN
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT-
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGN1F 1CANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
EME AN-CME AN=
EMEAK-CrtEAN=
EMEAN-CMt AN=
EMEAi4-CMEAN =
bME AN-CME AN=
ErtE AN-CME AN =
EME AN-CME AN •
EM£ AN-CME AN =
EME AN-CMc M -
EMEAN-CMEAN=
EMEAN-CMEAN =
tHEArt-CME AN=
EME AN-CME AN =
EHEAN-ChfcAN =
EMEAN-CMEAN=
EME AN-CME AN =
EMEAN-CMEAN'
EME AN-CME AN =
EME AN-CME AN =
EMEAN-CMEAN'
EME AN-CME AN =
E*EAN-CHE AN-
1.55
.05
-1.75
1.85
-6.30
-.10
.75
-.95
-.35
1.60
.65
.15
-.00
-.05
33.30
-3.55
S.75
128.20
380.05
163.35
35.60
24.90
DEG =
DEG =
DEG =
UtG =
ue&=
DEG =
DEG =
DEG =
UEG-
DEG =
OEG =
UEG =
UE& =
DEG =
DEG =
DEk,=
L)E&.
DEG-
DEG =
DE& =
DE& =
DEG =
2
2
2
2
2
2
2
2
2
i
4
i,
4
2
4
4
1
1
2
2
2
2
EMEAN=
EMEAN=
EMEAN-
EMEAN=
EMEAN=
ENEAN=
EMEAN=
EMbAN=
EMEAN=
EMEAN=
EMEAN=
EHEAN-
tMEAN=
EMEAN=
EMEAN«
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
5.00
4.40
4.45
34.90
42.30
13.60
4.35
4.55
4.45
36.95
12.65
15.40
4.90
4.40
4.45
36.90
46.45
8.65
9.70
170.70
420. £5
204.85
41.45
102.45
EDEV =
EDEV =
• t-*E-V-«
EOEV =
EOEV =
-EOEV^
EDEV =
feOEV=
EDEV =
EDEV =
EOEV-
EDEV =
-EOEV =
tDEV =
EDEV =
bOEV=
EDEV =
EDEV =
ED£V =
EDEV =
£4>£V-=
EDEV =
EDEV =
EDEV-*
.00
.00
.00
.00
.42
.57-
.07
.47
.07
.07
.49
1.41
.94
.78
.00
1.41
5.66
.76
.00
12.73
5-14 .00
.$0
39.39
4-^ft-
CMEAN=
CHEAN*
3.45
4.35
COEV =
CDEV =
CfrtAN* 4.45COEV-
CHEAN-
CHEAN=
CHEAN=
CMEAN=
CMEAN=
C«EAN»
CMEAN=
CMEAN-
ChEAN=
CMEAN-
CMEAN=
CMEAN=
CHEAN'
CMEAN-
CMEAN*
CMEAN=
CMEAN'
4MEAN-
CHEAN'
CMEAN'
C-MEAN*
36.65
40.45
19.90
4.45
3.80
CDEV =
CDEVc
COEV =
CDEV'
CDEV*
4.45CDEV'
37.90
13.00
13.80
4.25
4.25
4.45
36.95
13.15
12.20
3.95
42. SO
40.50
41.50
5.85
77.55
CDEV«
cot v=
CDEV«
CDEV«
COEV'
COEV»
CDEV«
CDEV»
CDEV«
CDEV«
COEV«
CDEV
CDEV«
COEV<
CDEV'
.07
.49
.00
.35
1.34
.57
.21
.28
.00
1.41
.99
.28
.21
.35
.00
.07
.78
.28
2.90
.00
8.20
29.98
.64
1.20
-------
FOULING ORGANISMS EXPERIMENT N0=l
DAY NUMBER 50
O
Ul
CADHIUM
CHROMIUM
COPPER
NICKIE
LEAD
ZINC
CADMIUM
CHRUH-IUM
COPPER
NICKLE
L-EAD
ZINC
T =
T*
TI
T =
T =
T =
DAY
T =
T«
7 s
TS
T"
T =
.00
.00
.00
.00
.00
.00
NUMBER
15.01
.82
19.23
1182.41
29.73
22.96
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
125
SIGNIFICANT
NOT SIGNIFICANT
SIGI.IF ICANT
SIGNIF ICANT
SIGNIF ICAUT
SIGNIF ICANT
EHfc AN-CME AN =
EMEAN-CME AN =
EHEAN-OHe-AN-
EME AN-CME AN=
tME AN-CME AN =
EMEAN-CME AN =
EMEAN-CHE AN =
EME/W-CHEfirt'
EMEAN-CMb AN*
EME AN-CME AN=
EMEAN-CWE Aw=
EMEAu-CMEAN=
-1
129
27
201
7
27
2
-t,
33
-2
10
161
.90 Ot(,-
.90 DEi> =
.30 UEG*
. 10 UEO =
.70 DEG=
.10 DfcG=
.60 DEG=
.10 OEo=
.30 DEG=
.00 U£G=
.30 DEG=
.05 [)EG =
0
0
0
0
0
0
1
1
1
1
1
1
EHEAN=
EMEAN =
EMEAN=
EMEAN=
EKEAN=
EMEAN=
EMEAN=
EMEAN=
EMEAN=
EHEAN=
EMEAN=
EMEAN=
1
209
82
306
13
109
4
42
70
69
27
224
.70 EDEV
.10 EDEV
.90 EOcV
.70 bDEV
.60 EDEV
.60 tDEV
.30 EDEV
.70 EUEV
.30 bOEV
.60 cUEV
.60 EDEV
.70 EDcV
14
.00
,00
,00
,00
,00
,00
00
.00
,00
,00
,00
.00
CHEAN=
CHEAN=
CHEAK*
CMEAN=
CHEAN=
CH£AN=
CHEAN=
CrtEAN'
CHEAN=
ChEAN=
CHEAN-
CHEAN=
3
7*
55
105
5
82
1
46
37
71
17
63
.60
.20
.60
.60
.90
.50
.70
.60
.00
.60
.30
.65
COEV
CDEV
CO£V
CDEV
CDEV
CDEV
CDEV
CDEV
CDEV
CDEV
CDEV
COEV
a
c
K
c
s
«
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*
f
X
=
=
.00
.00
.00
.00
.00
.00
.14
4.10
1.41
.00
.28
5.73
-------
SEDIMENT
CAY
CADMIUM
CHROMIUM
C-&PPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADM IUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHRQMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
MICKLE
LEAD
ZINC
T'
T»
TS
T'
T«
DAY
T =
Ja
1 =
T =
DAY
T =
T =
T'
T =
T«
DAY
T =
T =
T =
T =
T'
T*
DAY
1 s
T =
T =
T =
T'
DAY
T =
T =
T =
T =
T =
T =
NUMBER
1 .41
.62
4.00
.37
2.09
NUMBER
1 .41
1.00
.01
.26
NUMBER
.45
1.29
1.40
1.73
2.57
NUMBER
1.00
.47
.05
J.71
.43
.59
NUMBER
.97
1.79
.74
.00
1.41
NUMBER
2.61
.81
1.56
.04
.83
1.25
EXPERIMENT N0=l
0
EMtAK'CP.EAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
flCT SIGNIFICANT
NUT SIGNIFICANT
1
EMEAN 'CKEAN
EMEAN =CKEAN
fiCT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
5
EMEAN=CHEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
25
NOT SIGNIFICANT
NGT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
50
KOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN=ChEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NGT SIGNIFICANT
125
NOT SIGNIFICANT
NOT SIGNIFICANT
i«TJT SIGNIFICANT
NGT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN-CMEAN=
EMEAN-CME AN <•
EMEAN-CMt AN =
EMEAN-CMt AN=
EMEAN-CME AN =
EMEAN-CME AN =
EMEAN-CMEAN=
tMEAi\-CMt AN =
EHEAn-CMEAN=
ErtE AiM-CME AN =
EMEAu-CMEAN'
EMEAN-CME AN =
EMEAN-CMEAN'
EMEAu-CMt AN=
EMEAN-CrtE AN =
EMEAN-CMEAN=
EMEAN-CME AN =
ErtEAN-CMEAN=
EMEAN-CME AN =
EMEAh-CME AN=
EMEAN-CME AN =
EMEAN-CME AN =
EMEAN-CMt AN=
EMEAN-CMEAN'
EHEAN-CME AN =
EMEAN-CME AN =
EMEAN-CMEAN=
EME AN-CMfcAN=
EMEAN-CMEAN=
EMEAN-CMEAN'
EMEAN-CME AN =
1.00
.4:5
_/j >OQ
.95
3.15
-.95
-1.00
.05
.25
-.50
-1.00
-3.55
-4.95
-2.75
-.22
-.55
.27
-3.90
-3.05
-1.50
-1.45
1.70
-3.50
.00
-.85
-2.45
-1.20
-1.05
-.05
1.40
-3.50
DEG =
DEG>
OEG =
DEG =
OEG =
DEG =
L)EG =
OtG =
L>EG =
L/E& =
ut& =
DEG =
OEo =
UEG =
DEG =
DEo =
UtG =
DEG =
DtG =
u£G =
DEG =
DE& =
OtG =
DE& =
UtG =
DtG =
OtG =
DEG =
OEG =
DEG =
DEG =
EMEAN=
2 EMEAN=
2 EMEAN'
2 EMEAN=
2 EMEAN=
2 EMEAh-
thEAN-
EMEAN=
2 EMEAN=
2 EMEAU=
2 EMEAU'
2 EMEAN=
EMEAN=
2 ENEAN=
i EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN'
2 EMEAN'
2 EMEAN=
2 EMEAN=
2 EMEAN'
2 EMEAN=
EMEAN'
2 EMEAN=
2 EMEAN'
2 EMEAN=
2 EMEAN'
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
2 EMEAN=
.15
6.50
11.45
4.00
14.45
23.30
.15
7.50
10.45
8.00
14.95
20.95
.15
6.00
10.45
5.50
12.95
16.15
.15
6.95
10.45
6.95
13.90
19.65
.65
7.20-
4.45
9.40
10.00
18.40
1.95
10.70
10.45
16.90
11.95
18.25
EDEV =
EDEV =
eoev-
EDEV =
tDEV =
EOEV =
EDEV =
EDEV =
EDEV =
EDEV =
EOEV =
EUEV =
t&EV =
EDEV =
EOEV =
EDEV^
EDEV =
EDEV =
EDEV =
EOEV =
tDEV =
EDEV =
EDEV =
EDEV =
tDEV= '
EDcV =
EDEV =
EDEV =
EDEV'
EDEV =
EDEV
EDEV-
EDEV
EDEV =
EDEV =
EOEV =
.00
.71
.?•
.00
3.61
ir\.-l
2.12
2.12
.64
1.41
5.59
1.34
1.34
1.41
.78
2.12
2.90
1.46
.00
1.48
.64
.07
1.41
1.34
.07
.4-2
.42
.28
.00
.57
1.20
1.41
.64
1.41
2.05
1.63
CMEAN=
CHEAN'
CHfrAH*
CMEAN'
CMEAN'
CrtEAN*
CMEAN=
CMEAN'
CMEAN'
ChEAN'
CMEAN'
CMEAN'
CMEAN'
CMEAN'
CMEAN'
CMEAN=
CMEAN=
CMEAN'
CMEAN=
CHEAN'
CMEAN'
CMEAN'
CMEAN'
CMEAN'
CMEAN'
CHEAN'
CMEAN=
CMEAN'
CMEAN'
CMEAN=
CKEAN=
CMEAN'
CMEAN'
CMEAN'
CMEAN=
CMEAN=
16
.15CDEV
5.50 CDEV'
11.00 CDEV*
8.00 COEV
13.50 CDEV'
20.15 COEV'
.15CDEV
7.50CDEV
11.40 CDEV
9.00 COEV*
14.90 COEV'
20.70 CDEV=
.15COEV
6.50 CDEV'
11.45 CDEV
9.05 CDEV
17.90 CDEV'
20.90 CDEV'
.37 CDEV
7.50 COEV'
10.17 COEV
10.85 CDEV°
16.95 CDEV'
21.15 COEV
2.10 CDEV'
5.50 CDEV'
4.45CDEV
12.90 CDEV'
10.00 CDEV'
19.25 CDEV
4.40 COEV'
11.90 CDEV'
11.50 CDEV
16.95 CDEV'
10.55 COEV=
21.75 CDEV
.00
.71
.00
1.41
.71
.21
.00
.71
.71
.00
2.83
.00
.00
.71
.79
2.90
2.89
.28
.32
.71
8.10
1.48
9.63
3.32
2.12
1.27
.00
6.65
.20
.64
.57
1.56
.71
1.34
1.20
3.61
-------
THALASSL LEAF EXPERIMENT N0=l
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN* 1.65 DEVIATION
CHROMIUM NO EXPERMtNT MEAN- 1.70 DEVIATION
COPPER NO EXPERMENT MEAN' 14.03- OE VI AT l'&W«
NICKLE NO EXPERMENT ri£AN= ».05 DEVUT1UN =
L6AD NO EXPERMENT MEAN= 11.50 DEVIATIbi-j'
ZINC NO EXPERMENT MEAN= 36.63 OEVIAYIOH*
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADM IUK
CHROMIUM
COPPER
MCKLE
LEAJ
ZINC
CADM IUM
CHROMIUM
CLPPER
MCKLE
LEAD
ZINC
CADM IUM
CHRUMIUM
CUPPER
NICKLE
LEAD
ZINC
DAY
T*
T =
T«
T*
T*
T*
DAY
T*
T*
Ts
T«
T*
T*
DAY
T«
T s
T*
TB
T s
T-
DAY
T*
T»
T«
T*
T«
T*
NUMBER
.33
3.11
10.76
2.99
12.16
4.65
NUMBER
.50
2.51
4.95
1.82
3.86
7.78
NUMdER
.85
4.55
3.2
2.19
6.67
3.31
NUMBER
4.81
15.48
7.01
4.29
22.24
9.24
1
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIF KAMI
SIGlxlF ICANT
SIGMF ICANT
5
NOT SIGNIFICANT
NOT SIGNIFICANT
SIG.41HC.ANT
NOT SIGNIFICANT
SIGNIFICANT
SIGtflF ICANT
25
NOT SIGNIFICANT
SIGMF ICANT
jIGNIFICAM
H0t SIGNIFICANT
SIGNIFICANT
SIGMF ICANT
50
SIGMF ICANT
SIGMF ICAuT
SIGMF ICANT
SIGNIFICANT
SI GN1F IC«NT
SIGNIF ICANT
EMEAN-CMEriN*
EME Art-CME AN *
£. 'IE AN-CME AN *
E-1E AN-CKE AN*
tMEAN-CMt AN «
EriE Aft-CrlE AN *
EHEAN-Crte AN*
c'tfc A,*-CME AN *
EMEAN-CME AN =
EM£A,»-CMc AN =
EME AN-CME AN'
EMc AN-CME AN *
EME AN-CME JN =
L.'IE AN-CME AN =
EME AN-CME -ij =
EME AN-CME AN =
fcHE An-CHt AN =
EME AN-CMt SN =
ertE AN-CME «N=
E.'IE AN-CME ANs
EMEArt-CMEAN*
fcME AN-CME AN =
trtfc Art-CMC M =
EME A,J-IME AN =
1.30 NUM= 3
.00 NUM= 3
1 .53 NUM= 3
7.46 i,JM= 3
2.04 wUM= 3
9.32 NUM= 3
-.22 0£G=
8.18 u£G=
6.47 OEU=
11.67 UEG=
17.15 DEG=
10.32 UEC,=
.42 OEl,=
-7.48 UEG=
7.62 OEG=
-2.35 0£G=
10.61 DEo=
10.27 oEG=
-2.24 UcG=
33.15 OEG=
12.85 D£G=
15.60 UE&=
IS. 60 DEG=
-4.45 DEG=
-5.43 DEG=
b«.73 OEG=
37.23 U£G=
27.90 UEG=
77 .08 u tG =
25.90 DEG=
6 EMEAN=
6 EM£AN=
6 EKEAN=
6 EM£AN=
6 EMEAN=
6 EMEAN=
5 EhEAN*
5 EMEAN*
5 EMEAN=
4 EMEAN*
5 EMEAH*
3 fcMEAN=
6 EMEAN*
t £MeAi>i =
3 trt£AN=
4 EMEAN*
5 £MEAN-
5 EMEAN=
4 £M£6K=
3 EMEAN=
4 EMEAN*
H £M£AN=
3 tMEArt*
2 EMEAN=
3.00
27.65
21.27
50.15
32.02
50.30
2.62
25.72
22.62
44. 25
31.07
45.97
4.04
65.60
27.05
47.50
35,60
26.70
4.70
94.60
59.73
70.70
95.33
54.70
EDEV =
EDEV =
EDEV =
ED£V =
EDEV =
cD£V =
EOcV-
fcOEV =
EDEV =
t i)E V =
EDEV =
6DEV =
EDEV =
t DtV =
I U£V =
EDEV -
EOcV =
EDEV =
£OEV =
E UEV =
EDEV =
fcOEV =
£D£V =
EDEV =
1.05
3.81
.95
7.28
2.30
3.74
1.16
2.57
2.05
1.72
.98
1.20
4 .42
3.11
4.71
9.50
3.92
1.47
1.14
5.66
8.73
10.15
4.3»
2.26
CMEAN=
CMEAN*
CfiEAN =
CnEAN-
CM£AN=
CM EAN =
CMEAN=
CMEAN=
CMEAN*
CMtAN=
CM EAN-
CMEAN-
CMEAN=
CHEAN=
CMEAN=
CMEAN'
CH£AN=
CHE AN-
CM£AN=
CMEAN-
CMEAN=
CMEAN»
CMEAN<
CMEAN'
17
3.22
19.47
14.80
38.47
14.87
39.97
2.20
33.20
15.20
46.60
20.47
35.70
6.27
32.45
14.2
31.90
16.60
33.15
10.13
35.87
22.50
42.80
16.25
28.80
COEV =
CDEV*
CDEV*
CDEV*
CDEV *
CDEV'
COEV*
COEV =
CO£V«
CDEV«=
CDEV«
COEV«
CDEV»
CDEV =
COEV-
CDEV*
CDEV*
CDEV«
CDEV
CDtVs
CUEV«
CDtV«
CDEV
COEV*
.84
3.63
.85
2.85
1.64
2.40
1.01
5.30
1.97
.28
•S.56
1.6V
2.87
9.83
0.0
.9?
3.00
2.05
1.59
3.15
2.91
4.90
2..19
3.25
-------
00
THALASSL ROOT EXPERIMENT N0=l
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN* .15 DEVIATlUh*
CHROMIUM NO EXPERHENT HEAN* 5.00 DEVIATION*
COPPER MO EXPERMENT HEAN- 4.45 DE-VI AT-KiN-
NICKLE NG EXPERMENT MEAN* .45 DEVIATION—
LEAD NO EXPERHENT HEAN* 15.50 DEVIATION*
ZINC NO EXPERHENT MEAN* 25.40 DEVIATION*
CADMIUM
CHROMIUM
COPPER
MCKLE
LEAO
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
CbPPER
NICKLE
LEAD
ZINC
CADMIUM
CHRJMHW
COPPER
NICKLE
LEAO
ZINC
DAY
T*
T«
y»
T*
T«
T»
DAY
T«
T*
T*
TX
T*
T*
DAY
T-
T*
T«
T«
T«
T*
DAY
T*
T*
T.
T-
T.
T*
NUMBER
.67
1.30
6.57
7.61
11.71
1.02
NUMBER
1.75
0.04
.73
1.81
4.80
1.21
NUMBER
.33
3.18
2.79
4 .64
.06
1.43
NUMBER
.34
3.93
5.68
4.42
1.36
1.69
1
NOT SIGNIFICANT
NCT SIGNIFICANT
SIGN IF I CANT
SlGttlF I CANT
SIGNIFICANT
NOT SIGNIFICANT
S
NOT SIGNIFICANT
NOT SIGNIFICANT
,,CI SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NCT SIGNIFICANT
25
rtCT SIGNIFICANT
SIGNIFICANT
SIGwJF ICArtT
SIGNIFICANT
NOT SIGNIFICANT
NCT SIGNIFICANT
50
riOT SIGNIFICANT
SIGftIF ICAiMT
SIGNIFICANT
SIGNIFICANT
SIGNlF KAMI
NOT SIGNIFICANT
EMEAN-CME Art*
EME Art-CME AN *
EMEAu-CME AN*
c'ME A,»-CME AN*
tMEAi»-CME*N =
EMEAN-CMt. AN*
EMEArt-CHcAN*
CiMEAN-CME AN *
EME Art-CrtE AN *
EMEArt-CMcAN*
E;iEAiN-CMEAN =
EMEAN-CMEAN*
EdEAN-CMEAN=
fcME AN-CMt AN=
£MEA,4-CHt Afc*
EMEAN-CMEA.N*
EMEArt-CMt AN*
EMEAN-CMt AN*
EHEA.4-CHEAN*
EME AJV-CHt A»»*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMtAN*
EMEAN-CHEAN*
.00 NUH* 2
1 .27 NUH= 2
.00 NUH= 2
.00 NOH* 2
.00 NUM* 2
2.55 NUM* 2
-.84
5.00
3.48
13.92
7.87
2.20
-1.16
3.00
-.67
3.78
9.45
-2.22
-.18
12.62
5.82
11.72
I-.13
5.97
.10
25.67
.6.97
16.03
32.25
9.30
DEG*
DEG-
DEG*
uEG*
OEG =
OEG*
DEG*
OEG =
DEo*
OEG*
DEG*
DEG*
DEG =
UEG*
UEG*
DEG*
DEG*
DEG*
tlEG*
DEG*
DEG*
DEG*
DEG*
DEG =
4 EKEAN*
4 cMEAN*
4 EMEAN*
4 EMEAN*
4 ESEAN*
4 tMfcAN*
6 EMEAN*
4 EMEAN*
6 EMEAN*
6 EMEAN*
4 EMEAN*
6 EMEAN*
6 EMtAN*
6 EMEAN*
6 EMEAN*
S EMEAN*
6 EMEAN*
6 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
1.15
26.15
15.85
43.50
28.65
34.15
1 .64
32.83
14.10
46.45
27.30
34.62
1.67
40.02
19.12
45.95
22.10
34.37
2.40
54.32
30.92
50.37
44.45-
37.15
EDEV*
bDEV*
bDEV*
bDEV*
EDEV =
cDEV*
EDEV*
cDEV*
bDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV* -
EOEV*-
EDEV*
EOEV =
EDEV*
EDEV*
E&cV-
EDEV*
EDEV*
EOEV-
EDEV*
.21 CrlEAN*
1.77 CMEAN*
.21 CMEAN*
3.66 IHEAN*
.35 CHEAN*
1.63 CMEAN*
1.29 CHEAN*
.12 CHEAN*
1.31 CHEAN*
3.30 CHEAN*
3.34 CHEAN*
3.06 CHEAN*
.64 CMEAN*
5.20 CMEAN*
3,74 CMEAN*
3.45 CHEAN*
1.97 CHEAN-
6.19 CHEAN*
.38 CHEAN*
9,68 CHEAN*
3.49 CHEAN*
4.81 CHEAN*
5,14 CHEAN*
7.15 CMEAN*
16
1,99 COEV*
21.15 COEV*
12.37 CDEV*
29.57 COEV*
20.77 COEV*
31.95 CDEV*
3.00 CDEV*
29.83 COEV*
14.77 CDEV*
42.67 CDEV*
17.85 CDfcV*
36.85 CDEV*
1.65 CDEV*
27.40 CDEV*
13.30 CDEV*
34.23 COEV*
22.22 COEV*
28.40 CDEV*
2.30 CDEV*
26.65 COEV*
13.95 CDEV*
34.35 COEV*
12.20 COEV*
27.05 CDEV*
1.67
5.04
.69
1.20
.67
2.71
.32
1.00
1.29
2.55
2.90
2.04
.66
6.00
1.65
3.07
3.30
1.50
.14>
1.06
3.32
.78
.14
2.76
-------
l£
URCHIN INTERN
CADM IUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CA.DM IUM
CHROMIUM
COPPER
MCKLE
LEAD
ZINC
CADMIUM
CHROMIUM
CCPPFR
MCKLE
LEAD
ZINC
CADM IUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADM IUK
CHROMIUM
COPPER
MCKLE
L-EAD
ZINC
CADMIUM
CHROMIUM
COPPER
MICK4.E
LEAD
ZINC
DAY
T =
T =
T =
T =
T =
T =
DAY
T =
T =
T =
T =
T =
T =
DAY
T =
T =
T =
r =
T =
T =
.DAY
f =
T =
T =
T-
T =
T =
DAY
T =
T =
^ =
T*
T =
T =
DAY
T =
T =
T =
T =
T =
T =
NUMdER
.14
7.54
3.56
10.58
3.06
.49
NUMBER
1.00
.16
1.80
2.03
.97
8.64.
NUMBER
2.04
2.79
1.64
4.75
6.13
12.65
NUMBER
.19
4.78
3.39
6.16
16.72
12.75
NUH3ER
3.43
1.82
6.42
2.73
10.95
7.51
NUMBER
1.28
11.99
7.59
2.51
19.08
7.24
0
NOT
SI
SI
SI
SI
NDT
1
i\OT
NDT
HOT
,«OT
NOT
SI
5
.MOT
SI
NtTT
SI
SI
SI
25
NOT
SI
SI
SI
SI
SI
50
SI
NOT
SI
NOT
SI
125
NOT
SI
SI
SI
SI
SI
EXPERIMENT NO-1
19
SIGNIFICANT
SIGMF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGN IF ICAUT
SIGNIFICANT
SIGMF I CAM
SIGNIFICANT
SIGMF ICANT
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGMFICANI
SIGNIFICANT
50
SIGMF 1CAHT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIF ICANT
SIGNIF ICANT
SIGNIFICANT
SIGMF ICANT
SIGNIFICANT
EMEAN-CME AN =
EMEAN-CMEAN=
£HE Ai4-tHfc M *
EME AN-CME AN -
E.IEA^-CME AN =
EMEA,4-CM£«N =
EMEAN-CME AN*
EME AN-ChE AN=
EME Aw-CME AN=
EilEAH-CME AN*
ciHE Ai\-CMc AN =
E^E AN-CME AN =
trtEA.-»-CHc AN =
EKE Ai^-CKE Mi -
EMc AN-CHE AU=
EMEAN-CMfc AN =
6.4E Ail-CtiE AN *
t*EA;t-CMEAN =
EMEA,J-CMCArt =
EMEAiJ-CHE AN*
EMEA.'4-CME AN =
EME Aw-CMt AN«
Ei1EA,4-CMEAN =
EME AiJ-CME AN =
EMEAN-CMt AN=
EME AiY-CHE AN =
fcME AN-CME AN =
bME Ai<-CME AN =
EME AN-CME AN «
EMEAN-CME AN =
EMEAN-CME AN =
EMEAN-CME AN »
EME AN-CME AN =
EMEAN-CME AN =
fcrtE AN-CME AN =
EME AN-CME AN =
.21
-12.40
-4.04
-19.97
-4.33
-1.75
-1.77
.10
4.62
-3.76
-1.80
16.50
-2.37
1.83
4.74
13.10
iO.60
36.27
-.20
13.25
8.27
16.85
21.90
43.50
-1.72
4.80
7.53
8.10
11.00
17.4
-1.03
12.57
15.61
f. 16
29.92
69.26
UEG*
DEG*
DEG>
JEG =
DEG*
JEG =
UEO-
DEG =
DEG =
UbG =
L»EG =
OEG>
UEG =
OEo =
L/bG =
UE& =
DEG<
DtG-
DbG-
JEG-
oEGa
DEG-
i)EG°
DfcG =
UEG>=
Ut& =
DE&«
DEG-
DEG*
OE& =
OEt-
DE& =
LlE& =
DEG:
OEG'
uEG»
4
t,
4
L,
n
4
e.
2
i
2
i
2
4
tMEAN=
EMEAN=
EMEAN =
£MEAN=
EHEAU=
£MEAN=
EMEAN'
EMEAN=
EMEAN=
EMEAN'
EM£ftN=
EMEAN=
EMEAN'
2.26
3.20
5.56
16.42
13.62
46.40
.15
9.85
11.65
21.40
17.75
61.20
1.07
12.90
13.90
39.75
28.20
72.00
4.37
24.97
22.00
39.55
39.60
90.90
2.30
36.50
25.95
55.85
31.10
94.75
3.82
20. 31
21.20
22.83
50.27
160.73
EOEV =
tDEV*
EDEV>
EDEV =
tOEV =
EDEV =
EOEV =
EDtV-
EUtV =
eOEV =
EDEV =
EUEV =
EOEV =
tD£V =
t OtV =
tDEV =
EDEV =
EDEV =
EDEV =
EOcV =
EOEV =
EDbV =
EDEV =
EDEV =
EDEV =
EUEV =
t DEV =
EDEV =
EOEV =
EDEV =
EOEV =
EOEV =
EDEV =
ED£V =
EDEV =
EDEV =
1.96
1.76
2.22
2.48
1 .00
4.81
.00
.64
.07
.00
2.33
2.69
1.31
1.41
2.83
.07
1.13
3.39
1.50
5.02
2.76
4.59
1.31
3.51
.14
.14
1.48
2.90
2.26
2.90
1.07
1.92
3.63
3.86
3.04
14.83
ChtAN=
CrtEAN-
CHEAN=
CMEAN=
C.4EAN =
CriEAN=
CHEAN=
CrtEAN'
CMEAN=
CMEAM
CME»N=
CMEAN=
CKEAN=
CMEAN=
CMEAN=
CMEAN=
CMEAN=
CMEAN=
CMEAN=
CMEAN =
C«EAN=
CMEAN=
ChEAN=
CMEAN=
CMEAN=
C«EAN=
CMEAN=
CMEAN=
ChEAN=
C(iEAN =
CHEAN=
CMEAN=
CHEAN=
CHEAN=
CMEAN=
CMEAN=
2.05
15.60
11.60
36.40
17.95
48.15
1.92
9.75
7.02
25.15
19.55
44.70
3.45
11.07
9.16
26.65
17.60
35.72
4.57
11.72
13.72
22.70
16.70
47.40
4.02
31.70
18.42
47.75
20.10
77.35
4.85
7.75
5.59
17.67
20.35
111.47
CDEV =
COEV =
COEV =
Cl/EV =
COEV =
COtV =
COEV =
cotv =
cotv =
COEV
CUEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
COEV =
COEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
CDEV =
COEV =
COEV =
.21
2.26
.71
.71
2.76
.07
2.51
.64
3.64
2.62
1.20
.28
1.35
.31
3.50
3.68
2.21
3.28
1.42
2.35
4.02
.70
1.94
5.85
.67
3.53
1.31
3.58
.29
0.071
1.51
.95
2.28
1.48
.57
14.81
-------
URCHIN
DAY
CADMIUM T*
CHROMIUM T*
COPPER
NICKLE T*
LEAD T*
ZINC T*
DAY
CADMIUM T*
CHRulMIUM T*
COPPER T*
MCKLE T*
LEAD T*
ZINC T*
DAY
CADMIUM T*
CHROMIUM T*
COPPER T=
NICKLE T=
LEAD T*
ZINC T*
DAY
CADMIUM T*
CHROMIUM T*
COPPER
NICKLE T*
LEAD T*
ZINC T*
DAY
CADMIUM T*
CHROMIUM T*
COPPER
NICKLE T*
LEAD T*
ZINC
D-AY
CADMIUM T*
SHELL EXPERIMENT NO-1
NUMBER
.87
.78
1.03
.14
1.29
NUMBER
2.Q6
.66
.00
1.74
2.36
.51
NUMBER
1.92
3.74
.00
1.66
3.53
1.09
NUMBER
.33
.69
.55
2.47
5.01
NUI'.BER
2.83
1.00
.46
.23
NUMBER
1.26
CHROMIUM T* 18022.40
COPPER
NICKLE T*
LEAD T*
ZINC T*
.90
a. 84
13.50
0
NOT SIGNIFICANT
NOT SIGNIFICANT
ErfbAN-CMEAN
NCT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
1
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
5
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
25
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN *CMEAN
NOT SIGNIFICANT
HOT SIGNIFICANT
SIGNIFICANT
50
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN =CHEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN *CME AN
125
NOT SIGNIFICANT
SIGNIFICANT
EKEAN=CMtAN
NOT SIGNIFICANT
SIGfcIF ICANT
SIGNIFICANT
EHEArt-CMEAM*
EMEAN-CMEAN*
EM E Art -C ME AH «
EMEArt-CMbAn*
bHEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAiM-CMEAN*
EKEArt-CMEAN*
EMEAH-CME AN*
EMEAw-CMEAiM*
bME Art-CMC Ai»-
EMEAN-CMEAN*
ErtEAiv-CKt AN*
EMEA.4-CME AN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAi4-CMEAN*
EMEAw-CHc AN*
EMEAN-CMEAN*
EMEAH-CMcAN-
EMEArt-CKEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAiM-CnEAN*
EMEAu-CMEAN*
EHEAN-CMEAN*
EMEAN-C.U.EAN*
EMEAN-CMEArt*
-1.47 DEG*
-.20 DEG*
-10.95 OEG*
1.20 DEG*
-.55 DEG-
3.70 DEG*
-.60 UEG-
-.00 UEG-
6.90 DEO*
-18.3V UtG-
3.09 OEG*
-3 .$4 uEG*
3.00 OEG*
.00 UEG*
-14.35 OEG*
13.55 OEG*
1.75 UEG*
-.10 DEG-
.65 OEG*
-1.45 OEG*
1.00 UEG*
-2.70 DEG*
-.60 PEG*
.05 DEG*
-.50 DEG*
-.55 DEG*
.20 DEG*
1.10 DEG*
.95 DEG*
6.75 DEG*
2.70 OEG*
2 EMEAN*
2 EMEAN*
EHEAN*
2 EMEAN*
2 EMEAN*
2 EMEAN*
S EMEAN*
5 EMEAN*
5 EMEAN*
5 EMEAN*
5 CMEAN*
5 EMEAN.
4 EMEAN'
4 EMEAN*
4 tKEAN*
4 EMEAN-
4 EMEAN*
4 EMEAN*
2 EMEAN*
2 EMEAN.
EMEAN*
2 EMEAN*
2 EMEAN*
2 EMEAN*
2 EMEAN*
2 EMEAN*
EMEAN*
2 EMEAN*
2 EMEAN*
EMEAN*
2 EMEAN*
2 EMEAN*
EMEAN*
2 EMEAN*
2 EMEAN*
2 EMEAN*
1 .82
4.65
4.45
11.65
43.70
11.45
3.85
3.00
4.45
10.10
44.66
15.54
.15
5.60
4.45
4.60
56.35
11.55
3.90
3.50
4.45
31.90
12.10
10.50
4.25
6.55
4.45
26.35
57.60
15.40
4.75
6.50
4.45
27.90
58.35
16.20
EOEV*
EOEV*
eoev-
EDEV*
EDEV*
EDEV*
EOEV-
EDEV*
EDtV*
EDEV*
EOEV*
EOEV-
EDEV*
EOEV*
EOEV*
EOEV*
fcDEV-
EDEV*
EDEV*
EOEV*
EDEV*
EDEV* .
EOEV*
EDEV*
EDEV*
EDEV*
EOEV*
EDEV*
EDEV*
EDEV*
eoEv*
EOEV*
EOEV*
EDEV*
EDEV-
EOEV*
2.37
.35
.35
15.06
12.45
.21
2.37
1.24
.00
5.28
10.37
8.11
.00
.42
.00
.85
.21
1.63
.00
.00
.00
1.41
.99
.71
.21
.-07
.07
.78
.00
-.00
.07
.00
.00
.14
.92
.28
CHEAN*
CHEAN*
CMEAN-
CKEAN*
CHEAN*
CMEAN*
CMEAN*
CMEAN-
CHEAN*
CHEAN*
CHEAN*
CMEAN*
CHEAN*
CHEAN*
CMEAN*
CHEAN*
CMEAN*
CMEAN*
ChEAN=
CHEAN*
CHEAN*
CMEAN-
CMEAN*
CHEAN*
CHEAN*
CMEAN*
CMEAN*
CHEAN*
CMEAN*
CMEAN*
CMEAN*
CHEAN*
CMEAN*
CHEAN*
CMEAN*
CHEAN*
20
3.30 CDEV*
4.85 COEV*
4.45CDEV*
22.60 COEV-
42.50 COEV*
12.00 CDEV*
.15 COEV*
3.80 COEV*
4.45 COEV*
3.20 CDEV*
63.05 COEV*
12.45 COEV*
3.69 COEV*
2.60 CDEV*
4.45 COEV*
18.95 COEV*
42.80 CDEV*
9.80 COEV*
4.00 COEV*
2.65 COEV*
4.45CDEV*
33.35 CDEV*
10.30 COEV*
13.20 CDEV*
4.85 CDEV*
6.50 COEV*
4.45CDEV*
28.85 COEV*
58.35 COEV*
15.40CDEV*
4.55 COEV*
5.40 CDEV*
4.45CDEV*
26.95 COEV*
51.60 CDEV*
13.50 COEV*
.28
.07
.00
.42
.71
.57
.00
.00
.00
.99
1.49
.07
2.46
1.04
.00
11.38
5.12
1.92
.42
1.34
.00
3.46
.28
.28
.21
.00
.00
1.34
3.32
.71
.21
.00
.00
1.4g
.57
.00
-------
ZOO PLANK SOLIDS
DAY NUMBER
CADM IUH
CHROMIUM
COPPER
N1CKLE
LEAO
ZINC
T =
T =
T =
7 =
T =
T =
.00
.00
.00
.00
.00
.00
DAY .NUMBER
CACM lUf,
CHROMIUM
COPPER
M I C K L E
LEAD
ZINC
T =
T =
T =
T =
T =
T =
.00
1 .34
1.37
1.29
32942.04
.00
25
NGT
rtGT
«GT
NCT
nOT
rtCT
50
i»CT
NOT
HOT
UUT
SI
NTT
EXPERIMENT NO = I
S1&N1FI CANT
SIGNIFICANT
SIGNIFICANT
SIGN1F1 CANT
S I G N 1 F I C AM
S 1 G N I F 1 C AN 1
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
Gi,lF I CAN T
SIGNIFI CANT
EMEAN-CHEAN*
EML An-CMfc kit =
ErtE Art-CHt AN *
tf-IE A,^-CMt ''N =
tME Ai'i-Crtc AN =
ErtE AH-CME AN =
ErtEAn-CME AN=
tME An-ChE Ai\ «
EME A.J-CME *N«
E/IE AN-CMt An«
EMEAn-CME AN=
EME AN-CME AN=
-
-75
-15
-46
59
-
-153
-40
-115
-39
-16
.60 UEG*
.40 CtG*
. 10 OEG*
.30 DEG*
.40 tJ t G *
.70 UEG'
.60 DEtj*
.90 L>EG>
. f5 UEo«
.20 UEGe
.40 DEG>
.40 UEG"
0 tMEAN=
0 EMEAN=
0 £nEAn'
0 cKcAiM'
0 cMbtN -
0 EMEAN^
0 EMEAN=
1 EMEAN-
1 EME AN -
1 bhEAN'
1 EMEAN=
0 EMEAri-
4.90
IBS. 40
74.60
201.80
15.70
126.70
2.30
10.00
19.20
49.50
7.80
30.40
EDEV-
El)EV =
EOEV-
EDEV =
EDEV =
bOEV =
EOEV*
EOEV»
EOEV =
EliEV =
EOEV =
EOEV =
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
CMEAN=
CMEAN=
CMEAN*
CME«N=
CMtAN=
CMEAN=
CMEAN=
CrtEAN=
CMEAN=
CHEAN=
CM EAN-
CM£AN=
21
5.70
260.80
89.70
248.10
15.30
67.00
2.90
163.90
60.15
164.70
47.20
46.30
COEV =
CDEV =
CDEV^
CO EV =
CD£V =
CUEV =
C D F V =
C 0 F tf —
COEW =
C06V =
CDEV =
CDEV =
.00
.00
.00
.00
.00
.00
.00
93.90
24.40
72.97
.00
.00
-------
to
CLAM INTERNAL EXPERIMENT NO'2.
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN' 2.13 DEVIATION'
CHROMIUM NO EXPERMENT MEAN' 3.73 DEVIATION'
COPPER NU EXPERMENT MEAN' 21.8-3 DEVIATION*
MCKLE NO EXPERMENT MEAN' 6.80 DEVIATION'
LEAD NO EXPERMENT MEAN' 3.72 OEVlATIuN'
ZINC NO EXPERMENT MEAN' 119.70 DEVIATION'
CADMIUM
CHROMIUM
COPPER
MCKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADM IUM
CHROMIUM
COPPER
MCKLE
LEAD
ZINC
CADM IUM
CHROMIUM
COPPER
MCKLE
LEAD
ZINC
CADMIUM.
CHROMIUM
COPPER
NICKLE
LEAu
ZINC
CADM IUM
CHROMIUM
COPPER
MCKLE
LEAD
IIM.
DAY
T =
T =
T =
T =
T =
DAY
Js
T =
T =
T =
T =
T'
DAY
T =
j =
T =
T =
1 =
T =
DAY
T =
T =
T =
7 =
T =
T'
DAY
T =
T =
T'
T =
T =
T =
CLAY
T'
T =
T =
T'
T =
T'
DUMBER
.35
10.56
1 .44
.63
14.29
NUMBER
1 .26
.11
2.50
1.80
.69
1.74
NUMBER
2.14
4.00
7.48
3.64
.25
.44
MUMBE.R
4.24
1.32
7.52
1.73
1.91
5.41
NUMBER
5.50
2.18
.83
3.62
.08
.67
NUMBER
11.02
1.25
15.28
.59
1.85
7.15
1
HOT SIGNIFICANT
EMEAN'CKEAN
SIGN IF ICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGMF ICANT
5
NCT SIGNIFICANT
NCT SIGNIFICANT
NCT SIGNIFICANT
NOT SIGNIFICANT
;JOT SIGNIFICANT
NOT SIGNIFICANT
25
NOT SIGNIFICANT
SIGMF ICAftT
SIGN IF ICANT
SIGNIFICANT
NCT SIGNIFICANT
NOT SIGNIFICANT
50
SIGMF ICAN1
NCT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NCT SIGNIFICANT
SIGiJlF ICANT
85
SIGMF ICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGMF ICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
125
SIGNIFICANT
NOT SIGNIFICANT
SIGUIF ICANT
.U'T SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
EMEAN-CMEAN'
bME AN-CME AN"
EME Ah-CMEJN*
ErtEAU-CMEAN'
EME AN-CME AN »
EHE AN-CME AN*
bME Ai»-CrtE Alu*
EME AN-CME a««
tHE AN-CMt «N*
EMt AN-CME AN'
tKE AN-CME AN -
EME Au-Crtb AN«
EMEA,«-CMt AN*
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CMEAN*
EME AN-CME AN =
EMEAN-CMEAN'
bME AN-CME AN=
EME AN-CME AN'
EMEAN-CMEAN'
EMEAN-CMEAN'
EME Ai4-ChE MN =
EMEAN-CMtAM*
EMEAN-CMEAN*
EMEAn-CME AN'
EMEAN-CMEAN'
EMEArt-CHE AN*
EMEAN-CMEAN=
EME AN-CME AN =
EME AN-CME AN»
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAi4-CMEAW'
.12 NUM« 3
.12 NUM* 3
1.10 NUM* 3
.36 UUM* 3
3.04 i»UM« 3
7.30 NUM- 3
-.03
-7.33
-.30
.27
42.87
-.37
.13
6.90
.77
1.13
30.07
.27
-.ao
6.97
-.77
.10
-3.00
-.20
.27
6.77
.10
1.77
39.23
.37
1.03
-1.60
.57
.03
2.93
.90
1.30
15.70
.27
.77
-41.40
UEG*
UcG*
OEG*
OEG*.
DEG«
DEG*
UEG*
DEG*
UEG*
DEG*
l»EG«
DEG*
DEG*
UEG«
OEG*
DEG*
OEG*
DEG =
DEG'
DEG*
OEG*
OEG =
DEG'
DEv« =
DEG =
UEG'
DEG'
DEG*
OEG*
DEG'
DEG =
DEG*
DEG =
DEG =
DEG*
4 EMEAN*
EMEAN-
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN'
4 EMEAN*
4 EMfcAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN'
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN'
4 EMEAN'
4 EMEAN'
4 EMEAN'
4 EMEAN*
4 EMEAN'
4 EMEAN=
4 EMEAN'
4 EMEAN'
4 EHEAN'
4 EMEAN*
4 EMEAN'
4 EMEAN*
4 EMEAN'
4 EMEAN*
1.13
1.70
20.13
5.80
4.27
150.50
1.33
3.47
23.07
5.20
6.37
1.19.47
1.83
4.00
20.20
5.90
5.23
90.00
1.57
4.17
20.03
3.90
5.23
100.67
1.40
7.13
22.20
6.37
5.37
106.20
2.40
4.00
27.43
5.87
4.10
90.77
EDEV*
EDEV-
EDEV'
EDEV'
EDEV*
EDEV*
EOEV =
bDEV*
EOEV =
tDEV
EDEV'
EDEV
EDEV'
EDEV'
EOEV
EDEV*
EDEV'
EDEV'
EDEV*
EO£V '
EDEV*
EDEV*
EDEV*
EDEV =
EDEV
EDEV*
EDEV'
EDEV
EDEV =
EDEV'
EDEV*
EDEV*
EOEV*
EDEV
EDEV
EOEV
.06 CHEAN*
.06 CMEAN*
.76 CHEAN'
.20 CMEAN'
.64 CHEAN*
2.60 CHEAN'
.25 CMEAN*
1.59 CHEAN*
4.45 CMEAN'
.53 CHEAN*
.98 CHEAN'
12.23 CHEAN'
.21 CHEAN'
.35 CMEAN'
1.25 CHEAN'
.20 CMEAN*
.4-0 CHEAN'
9.36 Ch-EAN =
.06 CHEAN'
.06 CHEAN*
.75 CHEAN'
.10 CHEAN'
1.50 CHEAN'
11.63 CHEAN'
.10 CMEAN*
.55 CHEAN'
1.11 CHEAN'
.21 CHEAN'
.55 CMEAN'
4.23 CMEAN'
.10 CHEAN'
.52 CHEAN'
1.46 CMEAN'
.59 CHEAN*
.10 CHEAN*
6.12 -CMEAN*
22
.17
COEV*
1.70COE V
27.47
6.10
4.00
107.63
1.70
3.33
16.17
4.43
5.23
89.40
1.57
4.80
13.23
6.67
5.13
93.00
1.77
3.90
13.27
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61.43
1.03
6.10
23.80
5.80
5.33
103.27
1.50
2.70
11.73
5.60
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132.17
COEV
CDEV*
CDEV'
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COEV =
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COEV*
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COEV
.15
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.44
1.46
1.74
.51
2.48
27.37
.06
.00
1.02
.31
.58
7.01
.06
.35
1.37
.00
.59
4.75
.06
.61
3.14
.17
.45
6.30
.10
1.73
1.01
.52
.71
7.95
-------
CLAM
CADMIUM
CHROMIUM
COPPER
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ZINC
CADMIUM
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T
T
T
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T
T
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T
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SHELL EXPERIMENT NG=2
DAY NUMBER 0
HO EXPERHENT MEAN* 3.87 OEVIATIUN=
NO EXPERMENT MEAN= 7.17 DEVIAT1JN*
NU EXPERHENT MEAN' 4.45 D€-¥t*T IOJ.«
NU EXPERMENT MEAN' 13.03 DEVIATION*
NJ EXPERMENT MEAN' 33.97 DEVIATION*
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EMEAN-CME AN
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T
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SIGNIFICANT
SIGNIFICANT
SIGNIFI CANT
EMEAN-CMEAN
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EMEAN-CMEAN
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NICKLE
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EMEAN-CMt AN
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23
.55 NUM= 3
1.27 NUM* 3
.00 NUH* 3
1.55 NUH* 3
1.46 NUM* 3
.87 NUM* 3
.13 DEG*
-.27 DEG*
-1.48 DEG*
-.23 DEG*
-2.33 UEG =
-1.37 OEG*
-.13 DEG=
-.27 OEG=
1.52 DEG*
-.77 DEG =
-.67 DEG=
.10 DEG=
.17 DEG =
.23 0 Et> =
1.33 OEG =
3.07 DEG=-
-.13 DEG*
-.03 DtG =
.07 DEG*
-2.43 DEG =
.93 UEG =
-.03 DEG=
.40 DEG =
,20 DEG*
-.43 DEG*
1.03 DEG=
.03 DEG =
.20 DEG*
.90 DEG*
-1 .13 UEG*
-.70 OEG=
4 EMEAN=
4 EMEAN=
4 EMEAN*
4 EMEAN=
4 EMEAN*
4 EMEAN=
4 EMEAN*
4 EMEAN*
4 EMEA\=
4 EMEAN*
EMEAN =
4 EMEAN=
4 EMEAN=
EMEAI\| =
4 EMEAN=
4 EMEAN=
4 EMEAN=
4 EMEAN=
4 EMEAN=
EMEAN=
4 EMEAN=
4 EMEAN=
4 EHEAN=
4 EMEAN=
4 EMEAN=
EMEAN=
4 EMEAN=
H EMEAN=
4 E M E A N =
4 EMEAN=
4 EMEAN=
EHEAN=
4 EMEAN=
4 EMEAN=
2.40 EDEV=
6.97 EOEV=
4.45 EDEV=
15.13 EDEV=
36.93 EOEV
2.13 EDEV=
2.67 EDEV=
6.97 EDEV
5.97 EDEV=
12.17
35.13
1.40
2.97 EDEV
8.00 EDEV
4.45 EOEV=
13.67 EDEV
34.77 EDEV
4.93 EDEV
2.07
7.27
4.45
8.13 EDEV
45.97 EDEV
3.63
4 EMEAN=
2.03
9.27 EDEV
4.45 EDEV
14.77 EDEV
48. 13 ECEV
2.43 EDEV
2.73 EDEV
6.50 EDEV
4,45 EDEV--
18.00 EDEV
43.90 EDEV
2.90
.20 CHEAN*
,74 CMEAN=
.00 CMEAN*
.81 CMEAN*
1.76 CMEAN*
1.27 CMEAN=
.21 CHEAN=
.57 CMEAN=
2.63 CMEAN=
.71 CMEAN*
.80 CMEAN-
.60 CMEAN*
.21 CMEAN*
.26 CMEAN*
.26 CMEAN=
.81 CMEAN*
.51 CMEAN*
1.85 CMEAN*
.31 CHEAN*
.81 CHEAN*
Jl CMEAN*
.80 CMEAN*
1,27 CHEAN*
.45 CHEAN*
.06 CHEAN'
.46 CMEAN'
.46 CHEAN*
.38 CHEAN*
2.76 CHEAN'
.91 CHEAN'
.06 CHEAN'
.00 CHEAN'
,00 CMEAN'
.40 CHEAN'
1.56 CMEAN*
CMEAN'
2.27 CDEV*
7.23 CDEV'
.93 CDEV'
.37 COEV
39.27 CDEV'
3.50 CDEV*
5.
15.
2.60 CDEV*
7.23 CDEV*
4.45 COEV
12.93 COEV'
80 COEV
35
l.«OCDEV«
2.87 CDEV'
7.83 CDEV
4.45CDEV
13.43 COEV*
33.43 CDEV
1.87 COEV'
2.20 CDEV'
7.30 CDEV'
4.45CDEV
8.07 COEV
48.40 CDEV
2.70 COEV'
2.07 COEV'
8.87 CDEV
4.45COEV
14.57 COEV'
48.57 COEV
1.40 CDEV'
2.70 CDEV
6.30 CDEV-
4.45CDEV
17.10 COEV
45.03 CDEV'
3.60 COEV
.29
.47
2.57
1.66
7.30
.87
.10
.15
.00
1.29
1.07
.00
.06
.68
.00
.23
2.77
.91
.10
.50
.00
.06
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1.11
.12
1.20
.00
K27
.00
.17
.61
.00
1.47
.65
2.00
-------
NOLDTHUR BODY EXPE/UHENT NO -2
DAY NUMBER 0
CADMIUM NO EXPE3MENT MEAN* 1.97 DEVIATION*
CHROMIUM till EXPERMENT MEAN* 1.70 DEVIATION*
COPPER NO EXPERKENT MEAN* 4.45 DEVIATlljN*
KICK IE NO EXPESMtNT hEMi* 6. BO DEVIATION*
LEAD NO EXPERMENT MEAN* 11.23 DEVIATION'
ZINC NU EXPERMENT MEAN* 5.27 OEVIATluN*
CADMIUM
CHROMNW
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADH IUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPP-ER
NICKLE
LEAD
ZINC
DAY
T*
T*
T*
T*
DAY
T =
T =
T =
T =
T*
DAY
T*
T*
T =
T*
T =
T =
DAY
T =
T
T«
T*
T*
T =
DAY
T =
T«
T*
T =
T =
DAY
NUM3ER
1.51
1.84
5.41
2.80
NUMfeER
3.02
2.20
5.79
.39
.70
NUMBER
.98
1.03
1.00
.95
1.50
1.11
NUMBER
.37
3.54
.00
.70
1.81
1.36
NUMBER
1.00
.71
4.91
.29
.07
NUMBER
T» 26214.40
T*
T =
T*
T«
14.41
14.48
1.98
3.00
1
NOT SIGNIFICANT
EHfcAN=CMEAN
EMEAN =CCEAN
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
5
SIGNIFICANT
NOT SIGNIFICANT
EMEAN=CK.EAN
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
25
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
50
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
85
NOT SIGNIFICANT
NOT SIGNIFICANT
EMbAN-CMEAN
SIGNIFICANT
NOT SIGNIFICANT
NOT SluMFKANT
125
SIGNIFICANT
SIGNIFICANT
EMEAN *CHEAN
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
EMEA.4-OIEAN*
cNEAN-CMEAN*
EME'An-CMEAN*
EMEAN-CHE Ai< =
tMEAN-CMEAN*
EHE Ain-CMt AN =
EMEAn-CMEAN*
tMEArt-CMEAN*
EMEAi.-CMeiN*
EMEAN-CHE AN«
EMEAK-CMEAN*
EMEA.i-CMEAN*
EHEAw-CMEAN*
EMEAN-CMEAN=
EMEAN-CMEAN*
EHEAN-CMEAN*
EMEArt-OiEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAi4-CM£AN*
EMEAN-CMEAN*
EMEAN-CHE AN=
ENEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN=
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
cMEAN-CMEAN*
EMEAN-CMEAN*
.15 NUH* 3
.00 NUH* 3
.00 NUM« 3
.35 NUM* 3
1.31 NUM* 3
.23 NUN* 3
.17 OEG-
.47 DEG*
2.83 UEG*
.47 OEG«
-.27 DEG*
-1.60 OEG*
-1.27 UEO-
-.43 UEG*
.23 UcG*
.17' 0£l,«
-3.63 OEu*
1.75 DEv>*
-1.40 OfcG*
17.07 UEG*
1.30 DEG*
.05 UEG*
.02 OEG*
.00 DEG*
-.32 UEG*
-7.»5 DEG«
1.55 DEGc
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-.27 OtG*
-.43 DEG»
.10 DEG*
.03 UEG*
.20 DEG*
7.27 DEG*
5.20 DEG>
1.83 DEG*
9.45 DEG*
4 EMEAN-
kMEAN*
EMEAN*
4 EMEAN*
4 EMEAN-
4 EMEAK*
4 EHEAN*
4 EMEA<«*
EMEAN*
4 EHEAN*
4 cMfcAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 fcHEAN-
4 EMEAN*
4 EHEAN*
3 EMEAN*
9 EMEAN*
3 EMEAN*
3 EMEAN*
3 EMEAN*
3 EHEAN*
4 EMEAN*
4 EMEAN*
EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
1.63
1.70
4.45
6.17
11.43
4.80
1.20
3.13
4.45
6.00
11.23
4.39
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5.10
6.20
3.67
21.47
9.10
.95
4.65
4.45
5.65
9.95
8.05
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5.37
4.45
6.17
13.57
6.70
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3.97
4.45
11.27
7.20
19.23
EOEV*
EDEV-
EUEV-
EDEV*
EOEV*
£DEV*
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EDEV-
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.15 CHEAN*
.15 CHEAN*
.15 CMEAN*
.35 CHEAN*
.25 CMEAN-
.17 CMEAN*
.00 CMEAN*
1.24 CMEAN*
1.24 CMEAN*
.20 CHEAN*
.47 CHEAN*
.15 CHEAN*
.21 CHEAN*
.60 CHEAN*
3.04 O4EAN=
2.06 CHEAN*
18.31 CMEAN=
.82 €MEAN=
.21 CHEAN=
-&7 CMEAN=
.00 CMEAN=
.35 CHEAN*
2.47 CMEAN*
1.91 CMEAN=
.Oi CH£AN=
.57 CHEAN*
.57 CHEAN=
.15 CMEAN'
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.70 CHEAN*
.00 CHEAN*
.87 CHEAN*
.91 CMEAN*
.29 CHEAN*
.00 CHEAN*
a.*O~£*E4N*
24
1.47 COEV*
1.70CDEV*
4.45COEV*
5.70 CDEV*
8.60 CDEV*
4.33 CDEV*
1.47 CDEV*
4.73 CDEV=
4.45COEV=
7.27 COEV*
11.67 CDEV=
4.10 COEV*
.47 CDEV=
S. 73 CDEV=
4.45 COEV*
5.07 CDEV*
4.40 CDEV*
7.80 CDEV*
.90 COEV*
3.83 CDEV*
4.45 CDEV*
5.97 CDEV*
17.90 CDEV»
6.50 CDEV*
.70 CDEV*
5.63 CDEV*
4.45CDEV*
6.60 CDEV*
13.47 CDEV*
6.67 CDEV*
.60 CDEV*
1.70 XDEV*
4.45CDEV*
6.07 CDEV*
5.3.7 CDEV«
9.90 CDEV*
.12
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.00
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.23
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1.86
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6.11
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1.85
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5.62
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.32
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.59
.51
.00
.00
.00
.55
1.61
4,63
-------
HQLOTHUR INTERNAL EXPERIMENT NO -2
CADM IUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPP'ER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
Z4NC
DAY NUMBER 0
NO EXPERHENT MEAN* 3.13 DEVIATION*
NO EXPERHENT MEAN* 14.33 DEVIATION*
NO EXPERHENT MEAN* 4.45 DtViA-TIfiN-
NO EXPERMENT MEAN* 19.97 DEVIATION*
NO EXPERMENT MEAN* 26.77 DEVIATION*
NO EXPERMENT MEAN* 26.97 DEVIATION*
DAY NUMBER 1
T- 3.46 SIGNIFICANT EMEAN-CMEAN
T* 20.88 SIGNIFICANT EHEAN-CMEAN
T* 2.00 NDT SIGNIFICANT EHEAN-CMEAN
T* 36.55 SIGNIFICANT EMEAN-CMEAN
T» .20 NOT SIGNIFICANT EMEAN-CMEAN
T* 4.64 SIGNIFICANT EMEAN-CMEAN
DAY NUMBER 5
T* .49 NOT SIGNIFICANT EHEAN-CMEAN
T* 5.43 SIGNIFICANT EHEAN-CMEAN
T- 7.61 SIGNIFICANT EHEAN-CMEAN
T* 16.07 SIGNIFICANT EMEA.4-CMEAN
T* 1.76 NOT SIGNIFICANT EHEAN-CMEAN
T» 2.56 NOT SIGNIFICANT EMEAU-CMEAN
DAY NUMBER 26
T* 1.55 NOT SIGNIFICANT EMEAN-CMEAN
T* 9.35 SIGNIFICANT EMEAN-CMEAN
T* 14.97 SIGNIFICANT EMEAN-CHEAN
T* .92 NOT SIGNIFICANT EHEAN-CMEAN
T- 2.48 NOT SIGNIFICANT EMEAN-CMEAN
T* ' 3.54 SIGNIFICANT EMEAN-CMEAN
DAY NUMBER 50
T* 1.00 NOT SIGNIFICANT EMEAN-CMEAN
T> 17.59 SIGNIFICANT EMEAN-CHEAN
T* 3.92 SIGNIFICANT EMEAN-CMEAN
T* 0.70 SIGNIFICANT EME Art-CME AN
T- 3.59 SIGNIFICANT EHEAN-CMEAN
T* 5.47 SIGNIFICANT EMEAN-CMEAN
DAY NUMBER 85
T> 9.66 SIGNIFICANT EMEAN-CMEAN
T- 7.56 SIGNIFICANT EMEAN-CHEAN
T- 34.66 SIGNIFICANT EHEAN-CMEAN
T> 5.80 SIGNIFICANT EMEAN-CHEAN
T- 29.62 SIGNIFICANT EMEAN-CMEAN
T- 9.66 SIGNIFICANT EMEAN-CMEAN
DAY NUMBER 125
T* 4.00 SIGNIFICANT EMEAN-CMEAN
T* 9.41 SIGNIFICANT EHEAN-CHEAN
T* 5.36 SIGNIFICANT EHEAN-CHEAN
T* 22.92 SIGNIFICANT EHEAN-CHEAN
T- 10.79 SIGNIFICANT EHEAN-CHEAN
T- 6.42 SIGNIFICANT EHEAM-CHEAN
25
.15 NUM*
.70 NUM*
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3.50 NUM =
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38.
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92.
23.
40.
48.
64.
B
51.
13.
39.
14.
50.
50
20
45
77
33
50
53
37
00
33
93
87
90
00
70
05
40
35
90
50
27
33
97
47
40
30
77
23
20
67
00
17
00
63
20
40
EOEV
EOEV
EDEV
EDEV
EDEV
EDEV
EDEV
EOEV
EDEV
EDEV
EDEV
EDEV
S
E
X
S
S
*
B
=
B
B
X
B
EDEV*
EDEV
EDEV
EDEV
EOEV
EDEV
EDEV
EOEV
EDEV
EDEV
EOEV
EDEV
EDEV
EDEV
EDEV
EOEV
EDEV
EDEV
EDEV
3
3
3
B
A
X
r.
B
B
X.
=
s.
•
B
B
B
*
.
EDEV*
EDEV
EOEV
EDEV
EDEV
B
•
•
m
.00 CHEAN*
.44 CHEAN*
.00 CHEAN*
.86 CMEAN*
.38 CHEAN*
.35 CMEAN*
.1? CMEAN*
2.43- CMEAN*
.53 CMEAN'
3.44 CHEAN*
1.14 CMEAN*
7.02 CMEAN*
.00 CHEAN*
.42 CMEAN*
.26 {MEAN-
1.06 CHEAN'
1.13 CMEAN-
.21 CHEAN*
.10 CHEAN*
3.72 CMEAN-
2.28 CMEAN*
8.21 CHEAN*
1.25 CMEAN*
2.92 CMEAN*
.00 CHEAN-
2.86 CHEAN*
.15 CMEAN*
.45 CHEAN*
1.75 CHEAN*
2.79 CHEAN-
.00 CHEAN*
1.51 CHEAN*
.53 CMEAN*
.76 CHEAN-
1.13 CHEAN*
.96 CHEAN-
1.70 CDEV«
21.00 CDEV*
7.55 CDEV*
6.47 CDEV-
9.40 CDEV*
46.97 CDEV*
1.47 CDEV*
46.17 CDEV*
16.tO CDEV*
37.47 CDEV*
13.tO CDEV-
47.23 CDEV*
.97 CDEV*
34.13 CDEV*
10.10 CDEV-
31.37 CDEV*
9.48 CDEV*
41.87 CDEV-
.%3 CDEV-
44.27 COEV*
14.90 CDEV*
27.50 CDEV*
7.60 COEV*
26.77 COEV*
.90 CDEV-
77.70 CDEV*
13.43 CDEV*
35.73 CDEV*
16.47 COEV
41.77 C0EV*
1.07 CDEV-
30.31 COEV*
9.87 CDEV-
20.13 COEV*
7.10 CDEV*
39.90 CDEV-
.10
1.13
2.69
.31
.17
2.76
.21
.51
.26
2.19
1.76
1.56
.06
1.53
.36
2.34
6.02
2.07
.06
1.62
.66
.67
1.04
.*&
.10
1.73
.49
1.27
.61
3.01
.12
3.52
.84
.93
.17
1.93
-------
MANGROVE BODY EXPERIMENT HO =2
DAY NUMbER 0
CADMIUM NU EXPERMcNT MEAN= .25 UEVIATIof; =
CHROHIUH NU EXPERMENT IUAN= 6.87 DEVIATION*
COPPER NU EXPEHMtNT MEAN' 4.45 DEVIATION-
NICKLE NO EXPERMENT MtAN= 9.70 OcVIATI(jN =
LEAD NU EXPERMENT MEAN' .87 JEVlATIb,» =
ZINC NO EXPEKMENT Mfc«N= 2.37 DEVlATluh=
CADMIUM
CHROMIUM
CUPPER
MCKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPEk
NICKLE
LEAD
ZINC
CADMIUK
ChROMIU!',
CiJPPER
MCKLE
LEAD
ZINC
DAY
T =
T«
T =
T*
T*
DAY
1 1
T»
T«
DAY
T«
T«
T«
T«
NUMBER
2.54
.53
3.87
1.14
1.82
NUMEEk
3.78
1.12
.24
NUMBER
1.39
2.98
3.06
2.39
5
rtCT SIGNIFICANT
NOT SIGNIFICANT
EMcAN=CMEAN
SIGKlF ICAf, F
NOT SIGNIFICANT
NCT SIGNIFICANT
50
EriEAN *CMt AN
SIGMF ICAMT
fc MEAu=CI-.c Ai,
NOT SIGNIFICANT
EMt AN =CKE AN
NOT SIGNIFICANT
125
ilOT SIGNIFICANT
SICiMlF ICAI, F
EM£AN=CKE An
J1CNIF ICA.,T
EMEAN=a't AN
NOT SIGNIFICANT
lrtLAH-CMtAN=
EMEAN-CMEAN=
Li1EAl\-CHt AN =
EKE AN-CrtE Art=
EMEAN-CKE AN=
EI4E AN-CMc AN =
EhE AN-CME AN-
tMEAN-CME Ai< =
tKEAn-CME AN=
EMEAN-CHEAN=
EME Art-CME AN =
EMEAIi-CMEAN =
.09 I'.UM= 3
.06 NUM= 3
.00 MUH= 3
.56 NUM= 3
1.07 NUK= 3
.84 NUM= 3
-.83
1.60
2.73
-3.67
-1.53
.33
-.17
.30
-.08
.47
-1.27
-1.17
OEG =
UEu =
DE& =
Ot& =
DtG =
DE& =
UE& =
OE& =
UEG =
DEG =
DEG =
u)EG =
4 tMEAN=
4 EMEAN=
E MEAN =
4 EKEAN=
4 EMEAN=
4 EMEAN=
bMEAN=
4 EMEAN=
E M E A N -
4 fcMEAN=
EMEAN=
4 EC,EAN =
4 EMEAN=
4 EMEAN=
EnEAN=
4 EMEAN=
c M E A N =
4 EMEAN=
.40
6.27
4.45
9.13
2.52
4.07
.15
4.83
4.45
3.47
.25
3.27
.25
7.07
4.45
4.13
.25
3.63
EOEV =
EDEV =
EUEV -
EDEV =
tDEV =
EOEV =
EOEV =
EDEV =
EDEV =
EDEV =
EDEV -
EDEV =
EDEV =
E DEV =
EDEV =
EOEV =
EDEV =
EDEV =
.00
.75
.75
.68
2.10
.23
.23
.15
.15
.15
.15
1.62
.
.09
.25
.25
.29
.29
.SO
CMEAN=
CMEAN=
ChEAN=
CMEAN-
CHEAN=
CMEAN-
CM£AN=
CMEAN=
CMEAN=
CMEAN-
tMEAN=
CMEAN=
CMEAN=
CMEAN=
ChEAN-
CMEAN'
CMEAN-
CMEAN'
26
1.23 CDEV=
4.67 CDEV«
4.45tDcV=
6.40 COEV-
6.18 CDEV-
5.60 CDtV-
.15CDEV=
4.50 CDtV=
4.45CDEV=
3.63 CDEVc
.25CDfcV=
2.97 CDEV*
.33 COEV'
6.60 COEV=
4.45CDEV".
5.40 COEV
.25COEV«
4.80 COEV«
.57
5.14
.00
1.01
5.18
1.44
.00
.00
.00
.21
.00
1.46
.06
.10
.00
.66
.00
.26
-------
MANGROVE STEM EXPERIMENT NO »2
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN= .40 DEVIATION*
CHROMIUM NO EXPERMENT MEAN" 1.70 DEVIATION^
COPPER NO EXPERMENT MEAN* 4.45 DEVIATION'
NICKLE NO EXPERMENT MEAN* 4.67 DEVIATION
LEAD NO CXCERMENT MEAN- 3.72 0EVIATILN=
ZINC NO EXPERMENT MEAN- 4.67 OEV1ATIL«=
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY
T«
T«
T«
T«
T°
T*
DAY
T«
T«
DAY
T«
T«
T»
T*
T«
T»
NUMBER
1.00
1.80
.00
5.28
13.83
2.55
NUMBER
.73
1.86
NUMBER
.41
.10
1.00
.23
1.00
1.63
5
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
rtOT SIGNIFICANT
50
EM£AN=CHEAN
EMEAN'CME AN
EMEAN=CKEAN
NOT SIGNIFICANT
EMEAN=CMEAN
NOT SIGNIFICANT
125
MOT SIGNIFICANT
fcQT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN-ChE AN=
EMEAN-CMt 4N=
EMEAN-CMEAN=
EME A,Y-CMfc AN =
EMEAu-CMEAN=
EMEAN-CMEAN=
EMEAN-CMEAN*
EMEAN-CMEAN=
fcMEAN-CME AN»
EMEA.j-CMEAN =
EMEA.4-CMEAN*
EMEAN-CMEAl\l =
EMEAN-CMEAN*
EMEAN-CHE AN -
.10 NUM= 3
.00 NUH* 3
.00 NUM- 3
.32 NUH= 3
3.04 i\iUM= 3
.15 hUr1= 3
-.
-1.
•
6.
6.
-6.
— .
— •
.
«
1.
-.
2.
-1 .
15 OEG=
68 UEG=
00 UEG=
85 DEG=
70 DEG=
20 OEG=
27 OEG=
90 UEG=
05 UEG=
10 UEG=
55 DEG=
07 OEG=
96 UEG=
43 UEG=
3 EMEAN=
3 EMEAN=
3 EMEAN=
3 EMEAN=
3 EMEAN=
3 EMEAN=
EMEA(* =
EMEAN=
EMEAN=
^ EMEAN=
EMEAN =
4 E M E A N =
4 EMEAN=
4 EHEAN=
4 EMEAN=
4 EMEAN=
4 EMtAN=
4 EMEAN=
.55
7.85
4.45
12.95
6.95
8.30
.15
1.70
4.45
2.60
.25
4.30
.40
3.10
6.00
4.13
3.23
7.77
EDEV =
EDEV =
EOEV =
EDEV =
EOEV =
EDEV =
EOEV =
Li)EVs
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EDEV =
EOEV =
EOEV =
EOEV =
EOEV =
.07
1.48
.00
2.33
.92
.85"
.85
.85
.85
.53
.53
.70
.00
1.23
2.68
.32
5.17
.65
CHEAN*
CMEAN=
CMEAN*
CMEAN=
CHEAN=
CM£AN=
CMEAN=
CMtAN=
CMEAN=
CHEAN=
CMEAN=
CMEAN=
CMEAN=
CMEAN=
CHEAN'
CHEAN=
CHEAN'
CMEAN>
27
.70 COEV«
9.73 CDEV*
4.45 CDEV«
6.10 COEV
.25 CDEV-=
14.50 CDEV>
.15COEV.
1.70COEV»
4.45CDEV»
2.87 CDEV«
.25CDEV*
5.20 CDEV«
.35 CDEV-
3.00 CDEV
4.45 CDEV"
4.20 CDEV«
.25 COEy»
9.20 CDEV«
.10
.93
.00
.56
.00
3.21
.00
.00
.00
.35
.00
.46
.18
1.14
.00
.40
.00
1.37
-------
00
MANGROVE
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADNIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY
NO
NO
NO
NO
NO
NO
DAY
T-
T-
T-
T-
T-
T-
DAY
T-
T-
T-
T-
DAY
T-
T-
T*
T-
T-
T-
LEAF EXPERIMENT NO-2
NUMBER
0
EXPERMENT MEAN* .37
EXPERHfcNT MEAN* 1.70
EXPERHENT MEAN* 4.45
EXPERMENT MEAN* s.s?
EXPERHENT MEAN* 2.85
EXPERHENT MEAN* 15.80
NUMBER
.36
4.82
6.53
.5*
3.30
1.95
NUMBER
a 0.64
1.11
1.51
.83
NUMBER
2.12
32.91
2.19
15.65
1.00
J.37
5
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT.
50
EMEAN 'CMEAN
EMEAN -CMEAN
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
125
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
DEVIATION-
DEVIATION*
DEVIATION-
DEVIATION*
DEVIATION*
DEVIATION^
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMfcAN
EMEAN-CMKAN
EMEAN-CMEAN
EMEAN-CMEAN
EUEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
EMEAN-CMEAN
28
.06 NUM* 3
.00 NUM* 3
.00 NUM- 3
.06 NUM = 3
2.37 NUM* 3
1.64 NUM* 3
-.07 DEG*
-8.45 DEG*
7.38 L)EG =
-.65 DEG*
5.22 UE&=
-2.10 DEG=
4.B2 DEG*
.20 UEG-
-2.60 DEG*
1.57 DtG*
-.10 DEG*
-1.90 DEG*
-3.47 DEG*
-2.33 OEG*
1.25 DEG*
-5.37 DEG*
3 EMEAN*
3 EMEAN=
3 EMfcAN*
3 EMEAN*
3 EMEAN*
3 EMEAN*
EMEAN*
EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
.73 EDEV*
1.70 EDEV-
11.83 EDEV*
6.60 EDEV*
5.47 £OEV*
10.10 EDEV*
.15 EMV
1.70 EDEV
9.27 EDEV*
2.67 bDEV*
.25 EDEV*
11.07 EDEV*
.37 EDEV*
1.70 EDEV*
6.00 EDEV-
2.43 EDEV-
1.50 EDEV*
7.57 EDEV-
.15 CMEAN-
.00 CMEAN-
1.52 CMEAN*
1.18 CMEAN*
2.12 CMEAN*
.80 CMEAN*
.80 CMEAN-
.80 CMEAN*
.40 CMEAN-
.06 CHEAN*
.00 CMEAN-
3.05 CHEAN*
.06 CMEAN*
.00 CHEAN-
2.68 CHEAN*
.06 CMEAN*
2.17 CMEAN*
.25 CMEAN*
.80 CDEV*
10.15 CDEV*
4.45 CDEV-
7.25 CDEV-
.25 CDEV*
12.20 COtV-
.15CDEV-
1.70COEV-
4.45 CDEV*
2.47 COEV*
2.15 COEV-
9.50 COEV-
.47 COEV-
3.60 CDEV-
9.47 COEV-
4.77 CDEV-
.25 CDEV-
12.93 CDEV*
.28
3.32
.00
1.34
.00
1.70
.00
.00
.00
.31
2.98
1.22
.06
.10
.55
.26
.00
2.75
-------
MANGROVE ROOT EXPERIMENT NQ*2
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN* .10 DEVIATION*
CHROMIUM NO EXPERHENT MEAN* 24.37 DEVIATION*
COPPER NO EXPERHENT MEAN* 10.37 DEVIATION*
NICKLE NO EXPERHENT MEAN* 32.57 DEVIATION*
LEAD NO EXPERMENT MEAN* 4.23 OEVIATIUN*
ZINC NO EXPERMENT MEAN* 22.97 DEVIATION*
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
ChRGMIUH
COPPER
NICKLE
LEAD
ZINC
DAY
T*
T =
T*
T*
T =
T =
DAY
T =
T »
T*
T =
T*
DAY
T =
T*
T*
T*
T*
NUMBER
10.84
1.53
1.41
18.59
2.12
14.76
NUMBER
10.13
19.63
11.23
.93
5.76
NUMBER
.87
80.30
2.35
4.49
1.72
5
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
t«OT SIGNIFICANT
SIGNIF KANT
50
EMEAN*CMEAN
SIGNIFICANT
SIGNIF ICANT
SIGNIF ICANT
NOT SIGNIFICANT
SIGNIFICANT
1 W
NOT SIGNIFICANT
SIGNIFICANT
EHEAN *CMEAN
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
EMEArf-CMEAN*
EHEAN-CHEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
ErtEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMtAN*
EMEAN-CMEAN-
EMEAN-CME AN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EKEAN-CMEAN*
.20 NUH* 3
3.70 NUH* 3
.21 NUH* 3
1.17 NUH* 3
.42 NUM* 3
2.48 NUM* 3
-.47
1.12
2.76
9.73
2.99
3.85
9.13
5.02
6.00
-2.68
3.50
-.10
23.33
1.33
5.77
3.60
DEG*
OEG*
DEG*
DEG*
OEG*
OEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
3 EHEAN*
3 EHEAN*
3 EHEAN*
3 EMEAN*
3 EMEAN*
3 EMEAN*
EHEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
.53
17.57
9.83
26.63
5.07
20.90
.33
16.87
10.27
10.47
2.55
15.93
.70
30.53
4.45
14.10
7.30
22.)3
EDEV-
EDtV-
EDEV*
EDEV*
EDtV*
EDEV*
tetv*
EDEV*
EDEV*
EOEV*
EDEV*
EDEV*
EDEV*
kDEV*
EDEV*
EDEV*
EOEV*
EMV*
.06
.23
.21
.49
.50
•35
.35
l.SO
.51
.•7
2.19
.67
.00
.35
.35
.20
.10
3.35
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHfcAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
29
1.00 CDEV*
16.45 CDEV*
7.07 COEV*
16.90 CDEV*
2.07 CDEV*
17.05 COEV*
.33CDEV*
7.73 COEV*
4.45 CDEV*
4.47 CDEV*
5.23 CDEV*
12.43 CDEV*
.80 CDEV*
7.20 CDEV*
4.45CDEV*
12.77 CDEV*
1.53 CDEV*
18.73 CDEV*
.00
1.34
3.71
.71
2. SB
.OT
.06
.42
.00
.31
4.47
.11
.20
.36
.00
.96
2.22
1.37
-------
MERITA INTERNAL EXPERIMENT NO -2
DAY NUMBER 0
CADMIUM NO EXPERHENT HEAN- 3.80 DEVIATION-
CHROMIUM NO EXPERMENT MEAN- 4.23 DEVIATION-
COPPER MO €X»EAM£NT ME*N- 4*rS7 0€V4*T-10H«
NICKLE NO EXPERHENT HEAN* 11.20 DEVIATION*
LEAD NO EXPERHENT HEAN- 15.90 DEVIATION*
ZINC NO tXPERHENT MEAN* 147.97 DEVIATION*
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
.,
to CADMIUM
0 CHROMIUM
CbPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADHIUH
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY NUMBER
T- .00
T- .00
T- .00
T- .00
T* .00
DAY NUMBER
T* .00
T- .00
T- .00
T- .00
DAV NUMBER
T- .00
T- .00
T- .00
T- .00
T- .00
DAY NUMBER
T- .00
T« .00
T- .00
T- .00
T- .00
T- .00
DAY NUMBER
T« .00
T- .00
T- .00
T- .00
T- .00
T- .00
DAY NUMBER
NU CONTROL
NO CONTROL
NU CONTROL
NO CONTROL
NU CONTROL
NO CONTROL
1
NOT SIGNIFICANT EMEAN-CMEAN*
EHEAN-CHEAN
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CHEAN*
NCT SIGNIFICANT EMEAN-CMEAN*
5
EMEAN*CHEAN
EMEAN'CKEAN
HUT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CHEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EME Ai<-CME AN =
25
NOT SIGNIFICANT EME AU-CME AN '
EMEAN'CMEAN
NOT SIGNIFICANT EMEAN-CHEAN*
NOT SIGNIFICANT EMEAN-CMtAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
50
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EHEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN'
NOT SIGNIFICANT bMEAN-CHEAN*
NOT SIGNIFICANT EHEAN-CMEAN'
NOT SIGNIFICANT EHE A.4-CHE AN =
65
NCT SIGNIFICANT EME Art-CME AN*
NOT SIGNIFICANT EHEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN'
NOT SIGNIFICANT EMEAN-CMEAN*
NCT SIGNIFICANT EMEAN-CHEAN*
NCT SIGNIFICANT EMEAN-CHEAN*
125
HEAN- 3.60 DEVIATION*
HEAN- 6.30 DEVIATION-
MEAN- 57.70 DEVIATION-
MEAN- 10.30 DEVIATION*
HEAN- 11.60 DEVIATION-
MEAN- 123.20 DEVIATION-
.42 NUM- 3
.15 NUH- 3
6.04 NUH* 3
.80 NUM* 3
.36 NUM* 3
30.28 NUH* 3
-.ZO OEG'
1.70 DEG*
-1.40 DEG*
-1.40 DEG*
-15.90 DEG*
-8.30 DEG=
2.58 DEG*
-1.80 DEG*
-48.80 UEG*
.20 DEG*
-5.70 OEG*
.20 UEG*
-.50 OEG*
-44.00 DEG*
2.10 DEG*
-2.20 DEG*
3.00 DEG*
1.90 DEC*
7.70 DEG*
86.40 DEG*
1.10 DEG*
-5.20 UEG*
26.00 DEG*
-4.10 DEG*
.20 UEG*
-22.20 DEG*
.00 NUH* 1
.00 NUH* 1
.00 NUH- 1
.00 NUM* 1
.00 NUM- 1
.00 NUM- 1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
-0
0
0
0
0
0
0
0
0
0
0
u
EMEAN*
EMEAN-
EMtAN*
EMEAN*
EHEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN'
EMEAh*
EMEAN*
EMEAN*
EMEAN'
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMtAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EHEAN*
EHEAU*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
1.40
1.70
11.30
7.30
7.90
112.20
1.60
1.70
53.60
9.00
7.00
127.50
1.90
1.70
43.30
6.00
7.70
132.70
4.40
5.20
67.80
15.70
18.30
193.80
2.60
4.80
100.90
7.80
8.70
120.60
EDEV*
EDEV-
EDEV
EDEV-
EDEV
EDEV
EOEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV'
EDEV'
EDEV*
EDEV'
EDEV'
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV
EOEV
EDtV
EDEV"
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00-
.00
.00
.00
.00
.00
• .00
.00
.00
.00
.00
.00
.00
.00
.00
.00
CHEAN-
CMEAN-
CHEAN-
CMEAN"
CHEAN"
CHEAN"
CHEAN-
CHEAN-
CHEAN-
CHEAN-
CHEAN"
CHEAN"
CHEAN-
CHEAN*
CHEAN'
CHEAN-
CHEAN-
CHEAN-
CHEAN-
CMEAN-
CHEAN-
CHEAN-
CHEAN-
CHEAN-
CHEAN-
CHEAN"
CHEAN-
CHEAN-
CHEAN*
CHEAN-
30
1.60 CDEV
1.70CDEV
9.60 CDEV
8.70 CDEV
9.30 CDEV*
126.10 CDEV
1.60COEV
1.70COEV
61.90 CDEV
6.50 CDEV
8.80 CDEV
176.30 CDEV"
1.70 CDEV
1.70CDEV
49.00 CDEV
5.80 CDEV
8.20 CDEV
176.70 CDEV
2.30 CDEV"
7.40 CDEV
64.80 CDEV*
13.80 CDEV
10.60 CDEV
107.40 CDEV
1.50 CDEV
10.00 CDEV
74. »0 CDEV
11.90 CDEV
8.50 CDEV
142.80 CDEV
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
-------
NERITA SHELL EXPERIMENT N0*2
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN' 3.77 DEVI AT I UN-
CHROMIUM NO EXPERMENT MEAN* 7.87 DEVIATION-
COPPER NO EXPERMENT MEAN- 4.45 DEVIATIflN*
NICKLE NO EXPERMENT MEAN* 12.10 DEVIATION*
LEAD NO EXPERMENT MEAN* 36.00 DEVIATION-
£JNC NO EXPERMENT MEAN* 4.97 DEVIATION-
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADHIUM
CHROMIUM
COPPER
NICKLE
L€AD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
C-OP-P-E-R-
NICKLE
LEAD
ZINC
DAY NUMBER
T* 1.79
T* .71
T* 1.75
T= .51
T= .23
DAY NUMBER
T= .36
T= 1.21
T= .53
T= .65
T= .06
PAY NUMBER
T= 2.04
T= .21
T= .63
T= .26
DAY NUMdER
T s .46
T= .62
T= .80
T= 1.79
T= 5.23
T* .38
DAY NUMBER
T = 2.12
T= .76
T= .44
T = .81
T= .80
T= 1.87
DAY NUMBER
NO CONTROL
NO CONTROL
NO CONTROL
NO CONTROL
NO CONTROL
NO CONTROL
1
NOT SIGNIFICANT EHEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
EMEAN =CMEAN
NOT SIGNIFICANT EME A.N-CME AN *
NOT SIGNIFICANT EHEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMtAN*
5
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
EMEAN =CMEAN
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT fcMF. AN-CME «N *
25
EMEAN =CMEAN
NCT SIGNIFICANT EMEAN-CMEAN*
E«EAN*CMEAN
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EME AN-CME1 AN *
NOT SIGNIFICANT EMEAN-CMEAN*
50
NOT SIGNIFICANT EHEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NCT SIGNIFICANT EMEAN-CMEAN=
NOT SIGNIFICANT EMEAN-CMEAN*
^SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EME AN-CME AN =
85
NCT SIGNIFICANT EME Au-CHE AN *
NCT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EMEAN-CMEAN*
NOT SIGNIFICANT EME AN-CME AN =
NOT SIGNIFICANT EMEAN-CMEAN*
125
MEAN* 2.4J DEVIATION*
MEAN* 9.63 DEVIATluN*
MEAN* 4.45 DEVIATION-
MEAN* 12.67 DEVIATION'
MEAN* 42.83 DEVIATION*
MEAN* 4.80 DEVIATION*
.45 NUH-
.90 NUM-
.00 NUH-
.78 NUM*
.35 NUM-
.15 NUM-
-.47 DEG-
-.57 DE&-
-1.27 DEG'
-.67 UEG«
.30 DEG'
-.10 DEG-
-.70 fJEG*
.53 DEG*
.93 DEG'
-.03 DEG«
-.90 DEG'
-.13 OEG'
-.60 DEG*
-.10 DEG«
-.07 DEG«
.33 DEG*
.33 DEG*
-1.07 DtG*
2.20 UEG*
-.10 DEG*
-.10 DEG*
.27 OEG*
-.13 UEG*
-.17 DEG=
1.57 OEG*
-1.10 DEo*
.06 NUh* 3
.12 NUM* 3
.00 NUH* 3
.50 NUM* 3
3.19 NUH* 3
.34 NUM* 3
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
EMEAN*
EHEAN-
EMEAN'
EMEAN*
EMEAN»
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EHEAN*
EMtAN*
tMEAk*
EHEAN*
EMEAN'
EHEAN'
EMEAN*
EMEAN"
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMtAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
EMEAN*
3.00
1.93
4.45
11.73
35.83
5.77
3.87
6.37
4.45
12.73
34.53
4.43
3.17
7.63
4.45
11.33
32.63
4.33
5.13
8.20
10.23
14.30
48.70
4.47
2.23
9.20
9.27
13.57
50.50
3.30
EDEV*
EDEV'
EDEV *
EDEV'
EOEV'
EOeV«
EDEV-
EDEV*
EDEV*
EDEV*
EDEV-
EDEV-
EDEV*
EDcV*
EDEV*
EDEV*
EDEV*
EDEV'
EDEV'
EOEV*
EDEV*
EDEV-
EDEV*
EDEV'
EOEV*
EDEV*
EDEV'
EDEV'
EDEV*
EDEV*
.00
.65
.65
.29
1.77
1.59
.12
.76
.76
.99
.92
.59
.59
.25
.25
.58
1.47
.31
.15
.46
.06
.66
.50
.32
.06
.36
.15
.15
.46
•36
CHEAN'
CHEAN'
CMEAN*
CMEAN*
CMEAN'
CHEAN*
CMEAN*
CHEAN*
CMEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CHEAN*
CMEAN*
CHEAN*
CMEAN*
CNEAN*
CMEAN*
CMEAN*
CHEAN*
CMEAN*
CMEAN*
CHEAN*
CHEAN*
CHEAN*
CMEAN*
CttbAN*
CMEAN*
CHEAN*
31
3.47 CDEV*
1.50 CDEV*
4.45CDEV*
13.00 CDEV*
36.50 CDEV*
5.47 CDEV*
3.97 CDEV*
7.07 COEV*
4.45CDEV*
12.20 CDEV*
33.60 CDEV*
4.47 CDEV*
3.17CDEV*
8.53 COEV*
4.45COEV=
11.47 CDEV*
33.23 CDEV*
4.43 COEV=
5.20 CDEV*
7.87 COEV*
9.90 COEV*
15.37 CDEV*
46.50 CDEV*
4.57 CDEV=
2.33 CDEV*
8.93 CDEV*
9.40 CDEV*
13.73 CDEV*
48.93 CDEV*
4.40 CDEV*
45
1.21
.00
1.22
1.40
1.61
.46
.64
.00
1.45
2.33
.76
.40
.72
.00
.91
.76
.59
.20
.81
.72
.60
.53
.32
.06
.49
.50
.32
3.37
.95
-------
to
OYSTER INTERNAL EXPERIMENT NO-2
DAY NUMBER 0
CADMIUM NO EXPERHENT HEAN- 1.77 DEVIATION-
CHROMIUM NO EXPERHENT HEAN- 10.40 DEVIATION-
COPPER NO EXPERHENT HEAN- 88.60 DEVIATION-
NICKLE NO EXPERHENT HEAN* 19.23 DEVIATION-
LEAD NO EXPERHENT HEAN- 7.23 DEVIATION-
ZINC NO EXPERHENT HEAN- 657.37 DEVIATION*
CADMIUH
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZIMC
CADMIUM
CHRUMIUM
COPPER
NICKLE
LEAD
ZINC
CADH IUM
CHR3MIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUH
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY
T*
T*
T*
T*
T*
T*
DAY
T*
T*
T*
T =
T =
T =
DAY
7 =
T-
T =
T =
T*
T =
DAY
T*
T =
T*
T*
T =
T*
DAY
T =
T*
T =
T =
T =
T =
DAY
fs
T =
T =
T =
T*
T*
NUMBER
11.50
2.50
7.82
3.15
3.08
13.12
NUMBER
8.50
1.56
4.78
1.25
.50
2.21
NUMBER
3.77
2.39
8.51
2.14
2.92
2.70
NUMBER
1.31
2.59
10.30
6.42
3.05
2.44
foUHBEK
.00
.00
.00
.00
.00
.00
NUMBER
3.66
1.63
1.46
1.64
2.74
5.46
1
SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
5
SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
NOT SIGNIFICANT
NGT SIGNIFICANT
NCT SIGNIFICANT
25
SIGNIFICANT
NCT ilGNIFICANT
SI GiMlF ICANT
NOT SIGNIFICANT
SIGNIF ICANT
NOT SIGNIFICANT
50
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
MOT SIGNIFICANT
8b
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NGT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
125
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN-CHEAN*
EMEAN-CHtAN-
EHEAN-CMEAN-
EMEAN-CMtAN-
EMEAU-CMEAN*
EMEAN-CMEAN*
EMEAN-CHEAN-
EMEAN-CMEAN-
EMEAN-CMEAN*
EMEA.4-CMEAiM»
EMEAN-CMEAN-
tMEAN-CMEAN*
EMEAK-CMEAN-
trtEAN-CHEAN-
EMEAN-CMEAN-
EMEA.i-CHEAN-
EMEAN-CHEAN-
EMEAN-CMEAN-
ErtEAU-CME AN*
EHEA«-CHEAN«
EHEAH-CMEAN-
EMEAN-CMEAN*
EMEAri-CMEAN-
EME A'4-CiMEM*
EMEAN-CriEAN*
EMEAN-CMEAN*
EMEAN-CMbAN-
EMEAM-CMEAN*
EMEAN-CKEAN*
EMEAN-CMEAN*
EME AM-CME AN-
EW EAN-C ME AN*
EMEAN-CMEAN*
EMEAN-ChE AN*
EMEAN-CMEAN*
EMEArt-CMEAN*
.06 NUN- 3
.53 NUN- 3
3.00 fcUH- 3
1.53 NUM* 3
3.14 NUM- 3
145.94 NUH- 3
2.10
-6.53
72.97
-12.93
4.90
425.50
.95
2.65
47.90
3.00
.60
112.50
1.90
5.93
77.20
8.47
6.30
172.17
1.25
13.00
90.05
9.45
5.55
132. 9G
4.60
11.40
421.50
14.00
16.70
1651.70
.95
11.15
91.90
8.50
9.25
637.10
UEG-
0EG-
DEG-
UEG-
UE6-
UEG-
UEG-
DEG-
DEG-
DEG*
DEG-
DEG-
DEG-
UEG*
DEG*
DEG*
OEG-
DEG-
DEG-
UEG-
DEG*
DEG*
OEG*
OEG*
DEG-
DEG*
OEG*
DEG*
DEG*
UtG*
DEG*
DEG*
i>EG*
DEC-
DEC"
DEG*
4 EMEAN*
4 EHEAN*
4 EHEAN*
4 EHEAN*
4 EHEAN*
4 EHEAN-
2 EHEAN*
2 EMEAN*
2 EMEAN*
2 EHEAN*
2 EHfcAN-
2 EHEAN-
4 EHEAN*
4 EHEAN-
4 tMEAN*
4 EHEAN*
4 EHEAN*
4 EHEAN-
1 EHEAN*
1 EMEAN*
1 EMEAN*
1 EMEAN*
1 EMEAN*
1 EMEAN-
0 EHEAN*
0 EMbAN*
0 EHEAN*
0 EMEAN*
0 EMEAN*
0 EHEAN*
1 EMEAN*
1 EMEAN*
1 EMEAN*
1 EMEAN-
1 EMEAN*
1 EMEAN*
4.90 EOEV*
5.90 EDEV*
158.77 EDEV*
12.30 EDEV-
12.47 EDEV*
920.80 EDEV-
4.65 EDEV-
12.95 EDEV*
147.10 EDEV*
22.00 cDEV*
9.70 EDEV*
642.70 EDEV*
5.90 EDEV-
21.23 EDEV*
223.60 bDEV*
26.43 EDEV*
14.63 EDEV-
884.77 EDEV-
3.80 EDEV*
29.80 EOEV*
214.20 EDEV*
25.90 EOEV*
10.90 EDEV-
842.40 EDEV*
7.40 EDEV*
18.40 EDEV*
720.80 EOEV*
25.00 EDEV*
23.70 EDEV*
2386.90 bDEV*
3.45 EDEV*
28.35 EDEV*
3)6.40 EDEV*
25.40 EDEV*
14.35 EDEV*
1028.60 EDEV*
.17 CMEAN*
.53 CMEAN-
5.26 CMEAN-
.53 CMEAN*
2.02 CMEAN*
11.33 CMEAN*
.07 CMEAN*
1.91 CMEAN*
14.14 CMEAN*
2.55 CMEAN*
1.13 CMEAN*
47.23 CMEAN-
.79 CHEAN*
3.90 CHEAN*
12.88 CHEAN*
6.70 CHEAN*
1.72 CMEAN-
98.48 CMEAN*
.00 CHEAN*
.00 CHEAN*
.00 CHEAN*
.00 CHEAN*
.00 CHEAN*
.30 CHEAN*
.00 CMEAN*
.00 CnEAN*
.00 CHEAN*
.00 CMEAN*
.00 CMEAN*
.00 CMEAN*
.21 CHEAN*
5.59 CHEAN*
51.34 CMEAN*
4.24 CMEAN*
2.76 CHEAN*
95.32 CHEAN*
32
2.80 CDEV*
12.43 CDEV*
85.80 CDEV*
25.23 CDEV*
7.57 CDEV*
495.30 CDEV*
3.70 CDEV*
10.30 CDEV*
99.20 CDEV*
19,00 CDEV*
9.10 CDEV*
530.20 CDEV*
4.00 CDEV*
15.30 CDEV=
146.40 COEV*
17.97 CDEV*
8.33 CDEV=
712.60 CDEV*
2.55 CDEV=
16.80 COEV*
124.15 CDtV*
16.45 CDEV*
5.35 CDEV*
709.50 CDtV*
2.80 CDEV*
7.00 CDEV*
299.30 CDEV*
11.00 CDEV*
7.00 COEV*
735.20 CDEV*
2.50 CDEV*
17.20 CDEV*
244.50 CDEV*
16.90 CDEV*
5.10 CDEV*
391.50 COEV*
.26
4.49
15.28
7.10
1.88
55.03
.14
1.41
.71
2.26
1.27
54.45
.36
1.81
9.01
1.50
3.32
49.81
.78
4.10
7.14
1.20
1.48
44.55
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
-------
OYSTER SHELL EXPERIMENT NO-2
DAY NUMBER 0
CADMIUM NO EXPERMENT MEAN- 3.07 DEVIATION*
CHROMIUM NO EXPERMENT MEAN' 7.47 DEVIATION'
COPPER NO EXPERMENT MEAN* 4,45 DEV 1 AT KJN-
NICKLE NO EXPERMENT MEAN* 18.27 DEVIATION-
LEAD NO EXPERMENT MEAN' 36.17 DEVIATION*
ZINC NU EXPERMfcNT MEAN* 3.60 DEVIATION*
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
r-1
lo
W CADMIUM
CHROMIUM
COPPER
MCKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
MCKLE
LEAD
ZINC
DAY
T-
T*
T =
T*
T =
T =
DAY
T*
T =
T*
T*
T*
T«
DAY
T =
T =
T =
T*
T =
T«
DAY
1*
T*
T-
T =
T*
.DAY
T*
T*
T*
T*
T*
T«
DAY
T*
T*
T*
T«
T*
T*
NUMBER
.29
3.25
1.00
10.58
.78
6.76
NUMBER
.94
.66
1.51
1.19
.74
2.02
NUMBER
3.46
4.07
2.00
2.22
.39
3.61
NUMBER
.25
1.00
.42
.62
.68
NUMBER
1.89
1.34
5.24
.23
.77
2.61
NUMBER
.71
4.11
1.37
5.72
.30
13.46
1
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
5
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NLT SIGNIFICANT
NOT SIGNIFICANT
25
SIGNIF ICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
50
NOT SIGNIFICANT
NOT SIGNIFICANT
E'HEAN*CfcEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
85
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NUT SIGNIFICANT
125
NET SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
NOT SIGNIFICANT
SIGNIFICANT
EHEAN-CMEAN-
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMF.AU-CMEAN*
EMEAN-CMEAN*
EMEAiJ-CMEAN*
EMEAN-CHt AH*
EMEAN-CMEAN*
EMEAN-CHEAN-
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMfcAN*
EMEAN-CMEAN*
EMEArt-CME*N«
EMEAN-CMEAN*
EMEAi4-CHEAN*
bMEAN-CMEAN*
EMEAN-CMEAN*
EMEAU-CMEAN*
EMEAi.-CMEAN*
EMEAN-CMEAN*
EMEAN-CME AN-
EMEAH-CME AN-
EMEAH-CMEAN*
EMEAN-CMEAN*
EMEA.J-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAU-CMEAN-
EMEAN-CMEAN*
EHEAN-CMEAN*
EMEAN-CMEAN*
EMEAi4-CMEAN«
EMEAN-CMEAN*
.06 NUH* 9
.15 MUM- 3
.00 HUM* 3
.42 NUH* 3
.45 NUH* 3
.46 NUH* 3
.07 OEG*
-2.30 DEG-
1.58 DEG*
-4.07 OEG-
-.63 DEG-
-6.03 OEG*
-.33 i)EG»
-.23 OEG-
-.27 DEG*
-1.60 DEG-
-.87 DEC-
2.00 DEG-
.40 DEG-
-3.17 DEG-
-3.2C UEG-
-3. OS UkG*
-1.50 DEG-
-1.00 DEG-
.03 DEG-
.47 UEG*
.23 DEG*
1.13 OEG-
.20 DEG*
-.17 DEG*
.90 LEG-
2.17 DEG*
.20 uEG*
-.70 DEG*
2.77 DEG-
-.03 DEG-
3.03 DEG-
.60 UEG-
3.03 DEG*
1.20 OEG-
5.10 DEG-
4 EMEAN-
4 EMEAN-
4 EMEAN*
4 EMEAN-
4 EMEAN*
4 EMEAN-
4 EMEAN-
4 EMEAN-
4 EMEAN*
4 EMEAN-
4 EMEAN-
4 EMEAN*
4 EMEAN-
4 EHEAN*
4 EMEAN*
4 EMEAN*
4 EHEAN*
4 EMEAN*
4 EMEAU*
4 EHEAN-
EMEAN-
4 EHEAN*
4 EMEAN*
4 EMEAN-
4 EMEAN-
4 tHEAN-
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EKEAN*
4 EMEAN*
4 EHEAN*
4 EHEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
3.10
9.20
6.03
17.20
36.07
4.03
3.03
12.90
9.60
17.63
33.77
8.70
3.10
12.30
4.45
15.03
33.87
5.70
1.83
10.17
4.45
13.60
47.70
7.13
1.63
12.87
13.10
14.70
50.23
18.47
2.33
24.33
10.37
24.13
40.40
16.67
EDEV*
EDEV-
EOEV-
EDEV-
EDEV-
EOEV-
EDEV-
EDEV-
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDkV-
EDEV*
EOEV-
EUEV-
EDEV-
EDEV-
EDEV*
EOEV-
EDEV-
EDEV*
EOEV*
EOEV'
EDEV*
EDEV*
EOEV*
EDEV-
EDEV*
EUEV*
EDEV*
EDEV*
EDEV-
EOEV-
.10 CMEAN-
.87 CHEAN-
2.74 CMEAN*
.53 CMEAN*
.72 CMEAN-
.21 CHEAN*
.40 CMEAN-
.26 CrtEAN*
.00 CMEAN*
1.58 CHEAN*
1.76 CMEAN*
1.54 CMEAN*
.17 CMEAN*
.40 CMEAN-
.00 CMEAN*
1.18 CHEAN*
3.37 CMEAN*
.20 CMEAN*
.12 CMFAN*
.59 CMEAN*
.59 CMEAN*
.70 CMEAN*
2.72 CMEAN*
.29 CMEAN'
.12 CMEAN*
.72 CMEAN*
.46 CMEAN*
.46 CMEAN*
1.42 CMEAN.
1.70 CMEAN*
.06 CMEAN-
1.17 CMEAN-
.75 CMEAN*
.76 CMEAN*
5.31 CMEAN*
.»• CMEAN*
39
3.03 COEV-
11.50 CDEV-
4.45 COEV-
21.27 CDEV*
36.70 CDEV'
10.07 CDEV*
3.37 CUEV*
13.13 CDEV*
9.87 COEV*
19.23 COEV*
34.63 COEV»
6.70 CDEV*
2.70 CDEV-
15.47 CDEV*
7.65 CDfcV
18.07 CDEV*
35.37 CDEV*
6.70 CDEV*
1.80 CDEV*
9.70 COEV*
4.45CDEV*
13.37 CDEV*
46.57 CDEV*
6.93 CDEV*
1.80 CDEV*
11.97 COEV*
10.93 COEV-
14.50 CDEV*
50.93 CDEV*
15.70 CDEV*
2.37 CDEV-
21.30 COEV*
9.77 CDtV-
21.10 CDEV*
39.20 COEV-
10.87 CDEV*
.38
.87
.00
.40
1.21
1.53
.46
.55
.31
1.70
1.01
.75
.10
1.29
2.78
2.05
5.14
.44
.20
.56
.00
.65
1.57
.42
.10
.91
.55
1.41
.70
.69
.06
.52
.12
.52
4.61
.64
-------
FOOLING ORGANISMS
DAY NUMBER 25
EXPERIMENT N0«2
34
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
T- 6.36 SIGNIFICANT
T- 2.19 NOT SIGNIFICANT
EMEAN-CHEAN*
EHEAN-CHEAN*
T* 1.71 NOT SIGNIFICANT f MEAN-CHE-AN*
T- 3.74 SIGNIFICANT
T- 15.32 SIGNIFICANT
T- .63 NOT SIGNIFICANT
DAY NUMBER 50
T- 3.27 SIGNIFICANT
T- 7.03 SIGNIFICANT
T- .41 NOT SIGNIFICANT
T- 4.87 SIGNIFICANT
T- 22.34 SIGNIFICANT
T- .54 NOT SIGNIFICANT
DAY NUMBER 85
T- .18 NOT SIGNIFICANT
T- 6.43 SIGNIFICANT
T* 3.75 SIGNIFICANT
T- 4.37 SIGNIFICANT
T- 69.53 SIGNIFICANT
T- 15.79 SIGNIFICANT
DAY NUMBER 125
NO EXPERMENT MEAN* 1.93
HO EXPERNENT MEAN- 217.40
EMEArt-CHEAN*
EHEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CMEAN*
EMEAN-CHEAN*
EHEAN-CHEAN-
EMEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CHEAN*
EHEAN-CMEAN*
EMEAN-CHEAN-
DEVIAT10N-
DEVIATION-
NO EXPERMENT MEAN- 44.27- DEVIATION*.
NO EXPERMENT MEAN- 227.67
NO EXPERNENT MEAN- 20.03
NO EXPERHENT MEAN- 60.80
DEVIATION-
DEVIATION-
OE VI AT ION-
.30 DEG*
65.40 DEG*
-3.27 DEG*
54.50 DEG*
23.73 DE&*
-3.23 DEG*
.50 DEG*
92.53 DEG*
-1.67 DE&*
56.43 DEG*
33.10 DEG*
3.60 DEG*
-.03 DEG*
156.63 DEG*
11.13 DEG*
37.53 DEG*
52.13 DEG*
35.90 DEG*
.06 NUH* 3
12.92 NUH* 3
1.55 NUH* 3
2.93 NUH* 3
.71 NUH* 3
2.69 NUH* 3
4
4
4
4
4
4
4
-------
SEWAGE
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
4EAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
DAY
NO
NO
NO
NO
NO
NO
DAY
NO
NO
NO
NU
NO
NO
DAY
NO
NO
NO
NO
NO
NO
DAY
NU
NO
NO
NO
NO
NO
DAY
NO
NO
NO
NO
NO
NO
DAY
NO
NO
NO
NO
NO
NO
DAY
NO
NO
NO
NO
NO
NO
NUMBER
EXPERME
NT
EXPERMENT
EXPERME
EXPERME
EXPERME
EXPERME
NUMBER
EXPERME
EXPERME
EXPERME
EXPERME
EXPERME
EXPERME
NUMBER
EXPERME
EXPERME
EXPERME
EXPERME
EXPERME
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
NT
EXPERMENT
NUMBER
EXPERME
EXPERME
EXPERME
EXPERME
EXPERME
NT
NT
NT
NT
NT
EXPERMENT
NUMBER
EXPERME
EXPERME
EXPERME
EXPERME
NT
NT
NT
NT
EXPERMENT
EXPERMt
NUMBER
EXPERME
EXPERME
EXPERME
EXPERME
EXPERME
EXPERME
NUMBER
EXPERME
NT
NT
NT
NT
NT
NT
NT
0
MEAN
MEAN
s
=
MEAN =
MEAN
MEAN
MEAN
1
MEAN
MEAN
MEAN
MEAN
MEAN
MEAN
5
MEAN
MEAN
MEAN
MEAN
MEAN
MEAN
25
MEAN
MEAN
MEAN
MEAN
MEAN
MEAN
50
MEAN
=
s.
-
=
=
=
=
=
=
=
=
=
=
=
=
=
=
=
=
=
=
=
MEAN =
MEAN
MEAN
MEAN
MEAN
65
MEAN
MEAN
MEAN
MEAN
MEAN
MEAN
=
=
=
=
r
=
=
=
=
=
EXPERIMENT
165
3666
2445
375
21
2507
56
1567
1374
279
21
11 93
58
1979
1016
324
16
1261
51
1845
1306
245
34
1205
66
1979
1449
229
57
1444
31
1253
884
237
77
857
.97
.87
.33
.60
.40
.60
.33
.57
.40
.40
.03
.00
.97
.43
.17
.40
.17
.57
.57
.97
.80
.43
.03
.63
.50
.07
.63
.60
.17
.57
.13
.47
.53
.60
.57
.17
DEV
DEV
DEV
I
NO
AT
IAT
I
DEVI
DEV
I
DEVI
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
I
I
I
I
I
I
I
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
IAT
I
AT
IAT
I
I
DEVI
DEV
DEV
DEV
I
I
I
DEVI
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
DEV
OEV
DEV
DEV
I
I
I
I
1
I
I
I
I
I
I
I
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
AT
IAT
=2
ION:
IDu
ION1
ION:
ION;
ION''
ION:
ION:
ION:
ION:
ION:
ION:
ION:
ION:
ION'
ION'
IGft:
ION:
ION:
ION:
ION:
ION:
ION:
ION'
I ON:
ION:
ION:
ION:
ION:
10 iV
ION
ION:
ION:
ION:
ION
ION:
125
NT
EXPERMENT
EXPERME
EXPERME
NT
NT
EXPERMENT
EXPERMENT
MEAN
MEAN
MEAN
MEAN
MEAN
s
=
=
=
=
17
658
502
139
19
MEAN- <|31
.97
.73
.50
.57
.90
.40
DEV
DEV
DEV
DEV
DEV
DEV
I
AT
IAT
IAT
I
I
AT
AT
IAT
ION
ION
ION
IOK
ION
ION
85.45 NUM= 3
338.46 NUH- 3
266.43 NUM = 3
12.67 NUM= 3
3.29 NUM- 3
122.13 NUM= 3
6.07 NUM= 3
272.14 NUM= 3
183.97 NUM= 3
36.28 NUM- 3
4.87 NUM= 3
154.57 NUM= 3
5.13 NUM= 3
198.34 NUM= 3
36.58 NUM= 3
15.70 NUM= 3
4.24 NUM= 3
278.32 NUM= 3
5.55 NUM= 3
307.45 NUM= 3
304.00 NUM = 3
18.41 NUM= 3
14.56 NUH= 3
238.87 NUM= 3
8.32 NUM= 3
141.88 uUH= 3
37.69 NUM= 3
12.66 NUM= 3
5.46 NUH= 3
74.36 NUM= 3
8.56 NUM= 3
32.51 NUM= 3
9.31 NUM= 3
17.57 NUM= 3
16.23 NUM= 3
70.63 NUM= 3
1.04 NUM = 3
8.11 NUM- 3
57.23 NUM* 3
9.31 NUM- 3
.00 NUM= 3
22.85 NUM= 3
125
-------
SEDIMENT
DAY NUMBER
CADMIUM
CHROMIUM T- 1.46
COPPER
NICKLE T- 9.50
LEAD T- 8.00
ZINC T- .40
DAY NUMBER
CADMIUM
C-HROHIUM T- 7.18
COPPER
NICKLE T- .18
LEAD T- 1.57
ZINC T- 1.38
DAY NUMBER
CADMIUM T- .38
CHROMIUM T= 1.36
COPPER
MCKLE T- 2.54
LEAD T= 1.85
ZINC T- .14
DAY NUMBER
CADMIUM
CHROMIUM T- 2.54
COPPER T- 2.56
NICKLE T- 8.76
LEAD T = .08
ZINC T- 2.05
DAY NUMBER
CADMIUM T- 1.00
CHROMIUM T= .90
COPPER T- .09
NICKLE T- 1.71
LEAD T- 1.82
ZINC T- 3.13
DAY NUMBER
CADMIUM
CHROMIUM T- .33
COPPER
NICKLE T- .90
LEAD T- 1.30
ZINC T- .65
DAY NUMBER
CADM IUM
CHROMIUM T- 1.11
COPPER
NICKLE T- 2.96
LEAD T- 5.30
ZINC T- 4.SO
EXPERIMENT NO-2
EMEAN-CMEAN
NOT SIGNIFICANT
EMEAN-ChfcAN
.SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
1
EMEAN-CMEAN
SIGNIFICANT
EMEAN-CMEAN
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
NOT
NOT
5
NOT
NCT
SIGNIFICANT
SIGNIFICANT
EMEAN-CMEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
25
EMEAN-CKEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
50
NOT SIGNIFICANT
,-JOT SIGNIFICANT
NOT SIGNIFICANT
NET SIGNIFICANT
NOT SIGNIFICANT
SIG.MIF I CANT
85
1 EMEAN-CKEAN
NOT SIGNIFICANT
EMEAN-CMEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NCT SIGNIFICANT
125
EMEAN-CMEAN
NOT SIGNIFICANT
EMEAN-CMEAN
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
EHEAN-CMEAN-
EMEAN-CMEAN»
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
ErtEAU-CrlEArt-
EMEAN-CMEAN-
EMEArt-CMEAN-
EMEAN-CMcAN-
EMEArt-CMEArt-
EMEAN-CMEAN-
ErtEAN-CMEAN-
EMEAN-CMEAN-
EMEAu-CMEAN-
EMEAlj-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAu-CMt AN-
EMEAI.-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAiM-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAiM-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
EMEAN-CMEAN-
_
-a
-i
-
-i
_
-
_
-i
-i
-i
-
-
-i
-i
_
4
-
-
-
1
1
2
.33
.10
.1C
.07
.27
.17
.27
.73
.03
.07
.67
.30
.20
.90
.67
.80
.03
.40
.10
.4C
.20
.67
.63
.53
.50
.30
.43
.33
.47
.90
.00
.10
t)E'G»
DEO"
DEG-
DEG-
DEG-
DEG-
DEG-
DEG*
DEG-
UEG-
DEG-
DEO-
DEG-
DEG-
DEC-
DEG-
DEG-
l/EG«=
OEG-
OE& =
DEG-
DEG-
CiEG-
DEG-
OE&»
DEG-
UE&-
DEG-
LiEG-
DEG-
DEG*
DEG*
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
t
4
4
4
4
4
EV>
EDEV-
EDEV-
EDEV*
1.19
.25
.29
.21
.06
.12
.12
.26
.26
.06
.25
.35
.09
.40
.40
.30
.25
2.23
2.23
.46
.40
.35
.59
,«0
.00
.42
2.92
.64
.45
1.78
1.78
2.40
2.40
.40
.46
.25
.25
1.27
1.27
.47
.31
.61
CHEAN-
CMEAN-
CMEAN-
CMEAN-
CMEAN-
CHEAN-
CMEAN-
CMEAN-
CHEAN-
CMEAN-
CMEAN-
CMEAN-
CHEAN-
CMEAN-
CHEAN-
CMEAN-
CMEAN-
CMEAN-
CHEAN=
CHEAN-
CMEAN=
CMEAN-
CHEAN-
CMEAN-
CMEAN-
CMEAN-
CMEAN-
CMEAN<=
CHEAN-
CMEAN-
CMEAN-
CMEAN-
CHEAN-
CHEAN=
CMEAN-
CMEAN-
CMEAN'
CMEAN'
CMEAN*
CMEAN-
CMEAN-
CMEAN-
.60CDEV-
7.17 CDEV-
4.45CDEV-
9.37 COEV-
7.73 CDEV-
16.20 COEV-
.57CDEV-
6.97 CDEV-
4.45CDEV-
6.30 CDEV-
6.67 COEV-
15.93 COEV-
.28 CDEV-
6.73 CDEV=
4.45CDEV-
5.97 CDEV-
6.53 CDEV-
13.80 CDEV-
.47CDEV-
7.87 CDEV=
9.80 CDEV-
8.87 CDEV-
6.90 CDEV-
15.90 CDEV-
.40 CDEV-
6.57 CDEV-
5.93 COEV-
7.30 COEV-
4.90 CDEV*
12.07 CDEV-
.20CDEV-
42.67 CDEV
4.45CDEV-
8.33 CDEV-
6.77 CDEV-
15.47 CDEV>
.37COEV-
52.00 CDEV'
4.45CDEV*
10.37 CDEV«
7.17 CDEV-
15.43 CDEV-
.00
.31
.00
.32
.23
.26
.06
.15
.UO
1.65
.15
.85
.13
1.30
.00
1.10
1.19
1.05>
.06
.40
.20
.06
.36
.87
.17
.64
2.57
.20
.40
1.77
.09
1.05
.00
.42
.35
.85
.06
1.91
.00
.23
.12
.59
-------
THALASSL LEAF
DAY NUMBER
EXPERIMENT
37
CADMIUM NO EXPERMENT MEAN' 1.10 DEVIATION-
CHROMIUM NO EXPERMENT MEAN' 16.13 DEVIATION-
COPPER NO EXPERMENT MEAN' 4.45 DEVIATION-
NICKLE NO EXPERMENT MEAN' 29.40 DEVIATION'
LEAD NO EXPERHENT MEAN' 10.07 DEVIATION'
ZINC NO EXPERMENT MEAN' 27.63 DEVIATION-
DAY
CADMIUM T*
CHROMIUH T«
COPPER T*
MCKLE T*
LEAD T*
ZINC T*
DAY
CADMIUM T*
CHRUHIUH T*
COPPER T=
NICKLE T*
LEAD T-
Z1NC T*
DAY
CADMIUM T*
CHROMIUM T-
COPPER T»
NICKLE T*
LEAD T*
ZINC T*
DAY
CADMIUM 1'
CHROMIUH T>
COPPER T-
MCKLE T*
LEAD T-
ZINC i*
DAY
CADMIUM T«
CHROMIUH T«
COPPER T-
N1CKLE T*
LEAD T*
ZINC T-
DAY
NUMBER
.89
2.26
1.49
7.72
10.42
.24
NUMBER
.80
4.40
8.59
9.65
4.50
.35
NUMBER
4.16
23.57
6.54
17.56
10.73
t.83
NUMBER
8.19
4.93
2.59
8.67
5.27
1.86
NUMBER
a. 84
17.41
11.36
53.12
.59
3.13
NUMBER
CADMIUM T-»«»»**»»»
CHROMIUH T-
COPPER T-
NICKLE T-
LEAD
ZINC T«
9.37
16.62
13.87
3.12
1
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
SIGNIFICANT
NOT SIGNIFICANT
5
NOT SIGNIFICANT
SIGNIFICANT
SIG.1IF ICANT
SIGNIFICANT
SIGNIF ICANT
NOT SIGNIFICANT
25
SIGNIF ICANT
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGN IF ICANT
SIGNIF ICANT
50
SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
85
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
125
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
EMEAN 'CHEAN
NOT SIGNIFICANT
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN*
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CMEAN*
EMEAN-CMEAN'
EHEAN-CMEAN'
EMEAN-CMEAN=
tMEAN-CMEAN*
EMEAN-CMEAN'
EMEAIi-CME AN '
EHEAN-CMEAN*
EMEAN-CMfcAN*
EMEAN-CMEAN'
EMEAN-CMEAN'
EHEAN-CHEAN'
EMEAN-CHEAN'
EriEAN-CMEAN*
EMEAN-CMEAN'
EMEAN-CHEAN'
EMEAN-CHEAN'
EMEAN-CMEAN'
EMEA.*-CHEAN =
EHEAN-CHEAN'
EHEAN-CHEAN'
EMEAN-CMEAN*
EHEAN-CHEAN'
EHEAN-CHfcAN'
EMEAN-CHEAN'
EHEAN-CMEAN'
EHEAN-CMEAN*
EHEAN-CHEAN*
EHEAN-CHEAN-
.10 NUM« 3
.57 NUM« 3
.00 HUM- 3
1.30 NUM' 3
.64 NUH' 3
.91 NUH' 3
.25
2.32
.72
3.50
5.60
-.37
.23
14.03
3.73
14. 40
4.80
.80
.57
-5.37
-2.42
-12.23
7.6C
-8.67
3.10
-16.40
5.70
-17.13
3.67
6.70
.83
79.87
5.77
42.90
.13
6.87
2.30
21.97
14.53
7.57
3.30
DEG'
DEG-
OEG =
DEG'
DEG =
DEG'
DEG =
DEG'
DEG*
DEG =
DtG =
DEG'
DEG =
OEG =
t)E& =
l)EG =
DEG =
DEG =
DEG'
DE& =
DEG =
DEG*
DE& =
OE&'
OEG =
DcG'
DEG'
DEG'
DEG'
DEG«
DEG'
OEG'
DEG'
DEG'
DEG'
3 EMEAN*
3 EMEAN*
3 EMEAN'
3 EMEAN'
3 EMEAN'
3 EMEAN*
4 EHEAN'
4 EHEAN'
4 EMEAN'
4 EMEAN'
4 EMEAN'
4 EMEAN'
3 EMEAN*
3 EMEAN'
3 EMEAN*
3 EMEAN'
3 EMEAN'
3 EMEAN'
4 EKEAN'
4 EMEAN'
4 EMEAN'
4 EMEAN'
4 EHEAN'
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN'
4 EMEAN*
4 EMEAN'
2 EMEAN'
2 EMEAN*
2 EMEAN'
2 EMEAN'
EMEAN'
2 EHEAN-
1.70
15.47
10.87
24.20
12.20
25.83
2.30
53.30
18.97
56.63
14.27
37.33
4.20
32.00
20.15
29.50
7.85
23.20
5.80
39.53
30.60
27.10
13.77
47.07
3.47
124.87
29.17
73.67
6.60
31.80
3.50
73.20
29.60
36.60
.25
15.00
EDEV
EDEV
EDEV*
EDEV
EDEV
EDEV'
EDEV =
EDEV'
EDEV'
EDEV'
EDEV'
EDEV'
EDEV*
EDEV*
EDEV
EDEV =
EDEV'
EDEV
EDEV'
EDEV
fcDEV
EDEV'
EDEV'
EDEV'
EDEV
EDEV-
EDEV
EDEV
EDEV-
EDEV
EDEV*
EDEV-
EOEV
EDEV'
EOEV«
EOEV-
.35 CMEAN'
1.14 CHEAN'
.64 CMEAN'
.35 CMEAN*
.52 CHEAN'
.93 CMEAN'
.46 CMEAN'
5.24 CHEAN'
.72 CMEAN'
2.31 CHEAN'
1.72 CMEAN'
2.62 CMEAN'
.14 CMEAN*
.28 CMEAN'
.64 CMEAN*
1.27 CMEAN'
1.34 CMEAN'
.57 CMEAN'
.66 CMEAN'
4.54 CMEAN*
3.80 CMEAN*
3.42 CMEAN'
.91 CMEAN'
6.07 CMEAN'
.12 CMEAN*
6.91 CMEAN'
.12 CMEAN'
.31 CMEAN'
.26 CMEAN'
.62 CMEAN'
.00 CHEAN'
.00 CMEAN'
.00 CMEAN'
.00 CMEAN'
.00 CMEAN*
.00 CMEAN-
1.45 CDEV
13.15 CDEV
10.15 COEV*
20.70 CDEV
6.60 CDEV*
26.20 CDEV*
2.07 CDEV«
39.27 CDEV
15.23 CDEV*
42.23 CDEV
9.47 CDEV'
36.53 CDEV*
3.63 CDEV*
37.37 CDEV*
22.57 CDEV=
41.73 CDEV
.25 CDEV'
32.07 CDEV'
2.70 CDEV'
55.93 COEV«
24.90 CDEV
44.23 CDEV*
10.10 COEV'
40.37 CDEV'
2.63 COEV'
45.00 CDEV
23.40 CDEV-
30.77 CDEV-
6.47 CDEV
24.93 COEV*
1.20 CDEV'
51.23 CDEV*
15.07 CDEV
29.03 CDEV
.25CDEV
11.70 CDEV
.21
1.06
.07
.71
.71
2.55
.21
1.75
.21
1.15
.67
2.97
.15
.23
.21
.25
.00
1.29
.00
3.54
.10
.15
.79
1.11
.12
3.91
.87
1.37
.29
3.75
.00
2.03
.76
.47
.00
.92
-------
THALAS5L ROOT
DAY NUMBER 0
EXPERIMENT NO«2
30
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
H CHROMIUM
~ CUPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM.
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
NO EXPERMENT MEAN* 1.20 DEVIATION*
NO EXPERMENT MEAN* 17.00 DEVIATION'
NO EXPERMENT MEAN* 4.45 DEVIATION-
NO EXPERMENT MEAN* 26.47 DEVIATION'
NO EXPERMENT MEAN- 10.13 DEVIATION'
NJ EXPERMENT MEAN* 34.93 DEVIATION*
DAY
T*
T*
T*
T =
T =
T =
DAY
T*
f s
T*
T =
T =
T =
DAY
T*
T*
T*
T*
T*
T =
DAY
T*
T =
T =
T*
T*
T*
DAY
T =
T*
T*
T =
T*
T*
DAY
T =
T*
fs
T*
T*
T*
NUMBER
1.77
1.80
.44
2.69
14.63
.03
NUMBER
.50
29.57
15.06
24.77
8.57
8.69
NUMBER
9.19
1.71
2.44
2.76
.04
5.75
NUMBER
1.07
6.71
9.41
1.98
7.87
4.53
NUMBER
3.13
7.43
.53
1.72
1.72
2.31
NUMBER
2.24
4.55
.78
1.92
1 7 .'05
14.49
1
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIF ICANT
NOT SIGNIFICANT
5
NOT SIGNIFICANT
SIGNIFICANT
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
25
SIGNIFICANT
NOT SIGNIFICANT
i^CT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
50
NOT SIGNIFICANT
SIGNIF ICANT
SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
85
SIGNIF ICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
ivLT SIGNIFICANT
NOT SIGNIFICANT
125
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGUIF ICANT
SIGNIFICANT
EMEAN-CMEAN'
EMEAN-CHEAN'
EMEAN-CMEAN'
EMEAM-CHbAN'
EMEAU-CMEAN'
EMEAN-CMEAN"
EHEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CHEAN'
EMEAN-CHEAN'
EHEAN-CMEAN'
EMEAN-CMEAN'
EHEAN-CMEAN'
EMEA,-4-CHtA,\'
EMEAN-CMEAN*
EME AU-CMbAN»
EMEAN-CHt AN*
EMEAN-CMEAN*
EMEAN-Chc AN-
EHEAN-CMbAN'
EHEAN-CMEAN'
EMEArf-CME AN'
EMEAN-CHEAN'
EMEA.»-CMbAN«
EHEAN-CHEAN'
EHEAN-CHEAN'
bMEAN-CHEAN*
EHEAN-CMEAN'
EMEAN-CMEAN"
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAN-CMEAN'
EHEAN-CMEAN*
EMEAN-CHbAN'
EMEAN-CMEAN'
.10 NUM' 3
1.15 NUM" 3
,00 NUM- 3
.98 NUM" 3
1.53 NUM> 3
.21 NUH- 3
.17
2.23
.40
4.83
7.67
-.07
.03
38.17
11.00
42.10
8.43
6.23
.43
-2.27
-1.03
-5.23
-.03
0.70
.13
6.77
1.93
-1.13
6.97
11.13
-.23
21.10
-.47
-4.07
1.87
7.30
.17
12.67
1.60
-2.37
-4.37
11.97
DEG-
OEG«-
DtG*
DEG*
DEG*
DEG*
DEG-
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
OEG*
DEG*
OEG*
OEG*
DEG>
iiEG-
DEG*
DEG*
DtG-
DEG*
OEG*
DEG*
CEG*
DEG-
DEG*
DEG-
UEG*
DEG*
DEG*
DEG-
DEG*
DEG-
UEG-
4 EMEAN'
4 EMEAN'
4 EHEAN'
4 EMEAN'
4 EMEAN'
4 EHEAN*
4 EHEAN*
4 EMEAN-
4 EHEAN"
4 EMEAN*
4 EMEAN-
4 EHEAN'
4 EHEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN'
4 EMEAN*
4 EMEAN*
4 EMEAN*
M EMEAN*
4 EMEAN*
4 EHEAN*
4 EMEAN*
4 EMEAN*
3 EHEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EHEAN*
4 EMEAN*
3 EHEAN*
4 EHEAN*
4 EMEAN*
1.23
24.33
10.53
32.80
15.97
40.67
1.10
70.83
21.30
75.47
16.87
49.53
1.47
39.63
13.53
36.67
8.53
39.80
1.37
51.60
"17.97
34.07
18.07
52.53
1.63
91.70
19.63
48.90
9.63
42.53
1.83
78.07
18.27
36.23
3.17
32.73
EDEV'
EDEV-
EDEV*
EDEV*
EDEV*
EOEV'
EDEV*
EDEV*
EOEV*
EOEV-
EDEV-
EDEV-
EDEV*
EOEV*
EDEV*
EOEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EDEV*
EOEV*
EDEV*
EDEV*
EOEV*
EDEV'
EOEV*
EOEV*
EOEV*
EDEV*
EDEV-
EDEV*
EOEV*
EOEV*
bDEV*
.06 CHEAN*
1.36 CHEAN*
1.30 CMEAN'
1.30 CHEAN*
.74 CHEAN*
2.25 CHEAN*
.00 CHEAN*
2.20 CHEAN*
1.23 CHEAN*
2.61 CHEAN*
1.52 CHEAN*
.90 CHEAN*
.06 CHEAN*
2.06 CHEAN*
.61 CHEAN*
1.97 CHEAN*
1.45 CHEAN*
1.68 CHEAN*
.15 CHEAN*
1.04 CHEAN*
.06 CHEAN*
.38 CHEAN*
.68 CHEAN*
3.33 CHEAN*
.12 CHEAN*
2.79 CHEAN*
1.42 CHEAN*
4.38 CHEAN*
.25 CHEAN*
.98 CHEAN*
.06 CHEAN*
2.30 CHEAN*
.45 CHEAN*
.87 CHEAN*
.23 CHEAN-
.38 CMEAN'
1.07 CDEV-
22.10 CDEV-
10.13 CDEV*
27.97 CDEV-
8.30 CDEV*
40.73 COEV*
1.07 CDEV*
32.67 COEV*
10.30 CDEV*
33.37 COEV*
8.43 CDEV*
43.30 CDEV*
1.03 COEV*
41.90 COEV*
14.57 CDEV*
41.90 CDEV*
8.57 CDEV*
31.10 CDEV*
1.23 CDEV*
44.83 CDEV*
16.03 CDEV*
35.20 CDEV*
11.10 CDEV*
41.40 COEV*
1.07 CDEV*
70.60 COEV*
20.10 COEV*
53.77 CDEV*
7.77 CDEV*
35.23 COEV*
1.67 CDEV*
65.40 CDEV*
16.67 CDEV*
30.60 COEV*
7.53 CDEV-
20.77 CDEV-
.15
1.67
.86
2.83
.59
2.97
.12
.42
.30
1.37
.78
.85
.06
1.01
.40
2.63
.15
2.01
.15
1.40
.35
.92
1.37
2.65
.06
3.60
.52
.40
1.66
5.39
.12
4.23
3.54
1.98
.30
1.38
-------
URCHIN
INTERNAL
EXPERIMENT N0»2
39
CADMIUM
CHROHIUH
COPPE*
MCKLE
LEAD
Z-INC
CADMIUM
CHROMIUM
COPPER
NICKLE
L-EAO
ZINC
CADMIUH
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
|3 CHRUMIUM
\0 COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHRUMIUM
COPPER
NICKLE
LEAD
ZINC
CADMIUM
CHROHIUH
COPPER
NICKLE
LEAD
ZINC
DAY NUMBER 0
NO EXPERMENT MEAN' 2.17 DEVIATION'
NO EXPERMENT MEAN' 7.37 DEVIATION-
NO EXPERMENT MEAN- 4.V* &€VK*TKfN»
NO EXPERMENT MEAN' 16.03 DEVIATION'
NO EXPERMENT MEAN' 19.47 DEVIATION-
NO EXPERMENT MEAN* 27.33 DEVIA4ION*
DAY
T*
T*
T*
T*
T*
DAY
T*
T =
T*
T*
T*
T*
DAY
T*
T =
T*
T-
TS
T*
DAY
T*
T*
T*
T*
T*
T*
DAY
T«
T«
T*
T«
T'
T«
DAY
T-
T«
T«
T-
T*
T«
NUMBER
1.41
7.77
3.17
2.05
1.51
NUMBER
7.78
5.00
8.46
9.00
5.06
4.01
NUMBER
4.95
6.79
7.17
10.53
4.49
8.05
NUMBER
1.00
2.40
2.71
5.13
.29
1.62
NUMBER
5.00
53.67
9.60
25.94
7.06
1.83
NUMBER
4.47
12.05
26.77
2.38
11.57
6.46
1
NOT SIGNIFICANT
SIGNIFICANT
EHEAN *CHEAN
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
5
SIGNIFICANT
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIF ICANT
SIGNIFICANT
25
SIGKIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
50
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
85
SIGNIF ICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
NOT SIGNIFICANT
125
SIGNIFICANT
SIGNIFICANT
SIGNIF ICAN-T
NOT SIGNIFICANT
SIGNIFICANT
SIGNIFICANT
EMEAN-CHEAN'
EMEAN-CMEAN-
EMEAN-CMEAN*
EMEAN-CMEAN.
EMEAN-CHEAN*
EHEAN-CMEAN-
EHEAN-CMEAN.
EMEAN-CMEAN*
EHEAN-CHEAN-
EMEAN-CHEAN*
EHEAN-CHEAN-
EHEAN-CHE AN*
EMEAU-CMEAN*
EMEAN-CHEAN*
EHEAN-CHE AN'
EMEAN-CMEAN.
EMEAN-CHEAN'
EHEAN-CHEAN*
EMEAN-CHEAN*
EHEAN-CMEAN*
EHEArt-CHEAN'
EHEAN-CHEAN'
EMEAN-CMcAN*
EHEAN-CHEAN*
EHEAN-CMEAN*
EHEAN-CMEAN*
EMEAN-CMEAN'
EMEAN-CMEAN'
EMEAU-CMEAN'
EMEAN-CMEAN*
EMEAN-CMEAN*
EHEAN-CHEAN'
EHEAN-CHEAN*
EMEAN-CMEAN*
EHEAN-CMEAN*
.06 NUM* 3
.21 NUM* 3
.00 NUM- 3
.45 NUM- 3
1.51 NUM- 3
2.79 NUM> 3
-.07
.93
1.23
2.40
-2.37
-.37
3.80
2.03
7.40
4.73
7.90
-.23
20.93
3.87
29.50
4.4U
10.20
.03
-1.3C
-.70
-3.20
-.20
-4.40
.17
42.33
4.97
25.90
5.57
.73
.33
10.63
10.97
3.83
23. BO
18.67
DEG'
DfcG-
DEG>
DE6-
OEG-
DEG'
DEG>
DEG'
DEG'
DEC-
DEC'
DEG'
DEG*
DEG*
DEG*
DEG«
DEG'
DEG«
DEG'
OEG>
DEG'
OEG'
DEG*
DEG*
DEG*
DEG*
DEG*
[)EG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG*
DEG'
4 EMEAN-
4 EHEAN'
EHEAN'
4 EHEAN'
4 EHEAN'
4 EHEAN'
4 EHEAN'
4 EHEAN'
4 EMEAN-
4 EHEAN'
4 EXE AN-
4 EHEAN'
4 EHEAN'
4 EHEAN*
4 EMEAN*
4 EHEAN*
4 EHEAN*
4 EHEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN'
4 EHEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
4 EMEAN*
1.97 EDEV'
8.93 EDEV'
4.45 EDEV'
20.10 EDEV'
20.07 EDEV*
24.43 EDEV'
1.77 EDEV'
18.70 EDEV'
11.33 EDEV'
39.17 EOEV'
18.17 EOEV'
43.20 EDEV'
1.43 EDEV'
38.33 EDEV'
15.07 EDEV*
58.83 EDEV'
26.37 EDEV'
41.40 EDEV'
2.20 EDEV*
13.90 EOEV*
11.00 EDEV*
16.23 EDEV*
23-.47 EDEV*
27.37 EDEV*
2.37 EDEV*
70.77 EDEV*
15.47 EDEV*
47.50 EDEV'
20.30 EDEV*
42.53 EDEV*
2.37 EDEV*
28.27 EDEV*
20.80 EDEV*
34.53 EDEV=
46.90 EDEV*
54.70 EDEV=
.06 CHEAN'
.06 CHEAN'
.06 CHEAN'
.10 CHEAN'
1.80 CHEAN*
2.62 CHEAN*
.06 CHEAN*
1.31 CHEAN*
.38 CHEAN*
1.19 CHEAN*
.25 CHEAN*
3.21 CHEAN*
.06 CHEAN*
2.95 CHEAN*
.81 CHEAN*
4.84 CMEAN*
.84 CHEAN*
2.05 CHEAN*
.00 CHEAN*
.60 CHEAN*
.44 CHEAN*
.78 CHEAN*
1.00 CMEAN*
3.13 CHEAN*
.06 CHEAN*
.45 CHEAN=
.23 CHEAN*
.95 CHEAN*
.87 CMEAN*
.06 CMEAN*
.06 CHEAN*
1.53 'MEAN*
.3- :EAN=
2.64 (.MEAN*
2.85 CHEAN*
3.95 CHEAN*
2.03 CDEV
8.00 COEV*
4.45CDEV*
18.87 CDEV*
17.67 CDEV'
26.80 CDEV*
2.13 CDEV*
14.90 CDEV*
9.30 CDEV*
31.77 CDEV*
13.43 CDEV*
35.30 CDEV*
1.67 CDEV*
17.40 CDEV*
11.20 CDEV*
29.33 CDEV*
21.97 CDEV*
31.20 CDEV*
2.17 CDEV*
15.20 CDEV*
11.70 CDEV*
19.43 CDEV*
23.67 CDEV*
31.77 CDEV*
2.20 CDEV*
28.43 CDEV*
10.50 CDEV*
21.60 CDEV*
14.73 CDEV*
41.80 CDEV*
2.03 CDEV*
17.63 CDEV*
9.83 CDEV*
30.70 CDEV*
23.10 CDEV*
36.03 CDEV*
.06
.20
.00
.67
.93
.70
.06
.10
.17
.78
1.60
1.15
.06
4.45
.46
.38
1.47
.78
.06
.72
.10
.75
.65
3.51
.00
1.29
.87
1.44
1.05
.69
.12
.06
.61
.90
2.14
3.07
-------
URCHIN
CADHIUH
CHROHIUH
COPPER
NICKLE
LEAD
ZINC
CADHIUH
CHRQNIUH
COPPER
NICKLE
LEAD
ZINC
CADHIUH
CHRJHIUH
COPPER
NICKLE
LEAD
ZINC
DAY
NO
NO
NO
NO
N.U
NQ
DAY
T*
T-
T =
T =
T =
DAY
T*
T-
T =
T =
T =
SHELL EXPERIHENT NU-2
NUH8ER
0
EXPERHENT HEAN- 2.93
EXPERHENT HEAN- 5.03
EXPERHENT HEAN- 4.45
EXPERHENT HEAN- 14.30
EXPERHENT HEAN* 31.63
EXPERHENT HEAN* 4.37
NUMBER
1.11
1.06
.62
1.74
1.94
NUMBER
.44
.21
1.37
1.56
11.62
DAY NUMBER
CAOHIUH
H CHROHIUH
0 COPPER
NICKLE
LEAD
ZINC
T =
T =
T =
T-
T-
.41
1.31
1.75
.67
.43
DAY NUK6ER
CADHIUH
CHROMIUM
CUPPER
NICKLE
LEAD
ZINC
T =
T =
T*
T =
T =
.25
.73
.55
.10
1.17
DAY NUMBER
CADI1IUH
CHROHIUH
COPPER
NICKLE
LEAD
ZINC
T-
T»
T-
T-
T-
.30
2.78
4.44
.40
.98
DAY NUH6ER
CADHIUH
CHROMIUM
COPPER
NICKLE
LEAD
ZINC
T-
T-
T-
T-
T-
1.58
1.40
1.68
1.23
2.60
1
NOT SIGNIFICANT
NOT SIGNIFICANT
EHEAN-CHEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
5
NOT SIGNIFICANT
NGT SIGNIFICANT
EHEAN-CHEAN
NGT SIGNIFICANT
NOT SIGNIFICANT
SIGNIFICANT
25
NOT SIGNIFICANT
NOT SIGNIFICANT
EHEAN-CHEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
50
NOT SIGNIFICANT
NOT SIGNIFICANT
EMEAN-CMEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
85
NOT SIGNIFICANT
SIGNIFICANT
EHEAN-CfiEAN
SIGNIFICANT
NCT SIGNIFICANT
nIDT SIGNIFICANT
125
NOT SIGNIFICANT
NOT SIGNIFICANT
EHEAN-CHEAN
NOT SIGNIFICANT
NOT SIGNIFICANT
NOT SIGNIFICANT
DEVIATION-
OEVIAT IOK-
OEVIATIQN-
DEVIATION-
DEVIATION-
DEVIATION-
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAri-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAtt-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAK-CHEAN
EHEAN-CHEAN
EMEAN-CMEAN
EHEAN-CHEAN
EMEAN-CMEAN
EHEAN-CHEAN
EHEAN-CMEAN
EHEAN-CHEAN
EHEAN-CHtAN
EHEAN-CKtAN
EHEAN-CHEAN
EMEAN-CMEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
EHEAN-CHEAN
40
.15 NUN- 3
.76 NUM- 3
.00 NUH- 3
2.03 NUH- 3
1.11 NUH* 3
.06 NUH- 3
.13 OEO-
.23 DEC-
-.30 DEG-
.13 DEG-
.47 DEG-
.13 OEG-
-.07 DEG«
-.53 DEC-
-1.5C DEG-
4.37 DEC-
.10 bEG-
.37 DEG-
-1.23 UEG-
1.53 DEG-
-.13 DEG-
-.03 DE(,«
-.37 OEG»
-.37 DEG-
.27 OEG*
-1.63 DEG-
.03 OEG-
-1.30 DEG-
-.53 DEG-
-.97 DEG-
4 EHEAN-
4 EHEAN-
EHEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
EHEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
t EHEAN-
fcHEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
4 EMEAN-
4 EHEAN-
EHEAN-
4 EHEAN-
EHEAN--
EHEAN-
4 cHEAN-
4 EHEAN-
EHEAN*
4 EMEAN-
4 EMEAN*
2.43 DEG- 4 EMEAN-
-.17 OEG-
1.00 OEG-
-1.63 DEG-
2.97 DEG-
3.03 OEG-
4 EHEAN-
4 EHEAN-
EMEAN-
4 EHEAN-
4 EHEAN-
4 EHEAN-
2.90 EDEV-
5.47 EDEV-
4.45 EOEV-
14.53 EDEV-
30.57 EDEV-
5.07 EDEV-
2.80 EOEV-
5.00 EOEV-
4.45 EDEV-
13.37 EOEV-
28.43 EDEV-
19.97 EDEV-
2.13 EOEV-
7.60 EDEV-
4.45 EOEV*
8.53 EOEV-
48.30 EDEV-
9.20 EOEV-
2.67 EDEV-
5.23 EOEV-
4.45 EDEV-
10.60 EDEV-
39.83 EDEV
13.43 EDEV-
2.77 EDEV-
8.13 EDEV-
4.45 EDEV-
14.00 EDEV*
43.03 EDEV-
12.BO EDEV-
2.50 EDEV-
6.80 EDEV*
4.45 EOEV-
15.07 EOEV=
40.00 EDEV-
14.43 EDEV*
.20 CHEAN-
.23 CHEAN-
.23 CHEAN-
.78 CHEAN-
1.03 CHEAN-
.12 CHEAN'
.46 CHEAN-
.26 CHEAN-
.26 CHEAN-
.65 CrtEAN-
1.46 CHEAN-
.55 CHEAN-
.40 CHEAN-
.36 CHEAN-
.36 CHEAN-
.67 CHEAN*
.89 CHEAN-
.53 CHEAN-
.21 CHEAN*
.61 CHEAN*
.61 CHEAN*
1.06 CHEAN-
.93 CHEAN-
1.65 CHEAN-
.12 CHEAN*
.50 CHEAN*
.50 CHEAN*
.17 CHEAN-
2.20 CHEAN*
1.56 CHEAN-
.10 CHEAN-
.79 CHEAN*
.79 CHEAN-
1.36 CHEAN-
1.41 CHEAN*
.68 CHEAN*
2.77 CDEV-
5.23 COEV-
4.4SCOEV*
14.83 COEV-
28.43 COEV-
4.60 CDEV-
2.67 CDEV-
5.07 CDEV-
4.45COtV-
13.90 COEV-
29.93 CDEV-
15.60 COEV*
2.03 CDEV-
7.23 CDEV-
4.45CDEV*
9.77 CDEV*
46.77 CDEV-
9.33 CDEV-
2.70 COEV-
5.60 COEV-
4.45CDtV=
10.97 COEV-
39.57 COEV=
15.07 CDEV*
2.73 COEV-
9.43 CDEV*
4.45COEV*
14.53 COEV*
44.00 CDEV*
10.37 CDEV*
2.67 CDEV*
5.80 CDEV-
4.45COEV-
16.70 CDEV-
37.03 CDEV-
11.40 COEV-
.06
.31
.00
.32
1.86
.00
.25
.49
»
.17
.81
.35
.12
.32
.00
1.03
3.89
.06
.10
.62
.00
.46
4.50
1.76
.15
.64
.00
.12
3.54
4.03
.15
.95
.00-
1.00
3.93
1.90
-------
APPENDIX C
Experiment II, Water Data
The column headings are defined as follows:
1.
E/C
2. METAL
3. MEAN PART
4. SD PART
5. MEAN SOC
6. SD SOC
7. MEAN SIC
8. SD SIC
E is data from experimental tank
C " " " control "
concentration (yg/fc) shown are for the defined
metal, either Cd, Cr, Cu, Ni, Pb or Zn
values shown represent mean (yg/A) concentration
for particulate fraction
represents the standard deviation of the mean
particulate function
values shown represent mean concentration (yg/&)
for the soluble organic fraction (Retained on
Amberlite resin)
represents the standard deviation of the mean
soluble organic fraction
values shown represent the mean concentration
(yg/&) of the soluble inorganic fraction
(Retained on Chelex-100 R resin)
Represents the standard deviation of the soluble
inorganic fraction
131
-------
/c
E
C
E
C
E
C
E
C
E
C
E
C
METAL
Cd
Cr
Cu
Ni
Pb
Zn
MEAN
0.
0.
3.
4.
1.
2.
4.
4.
0.
0.
1.
2.
PART
1
1
3
3
8
4
4
6
0
0
6
6
SD PART
0. 1
0.1
3.1
U.I
1.2
2.0
3.9
3.9
N/A
N/A
0.78
1.8
MEAN
0
0
0
0
0
0
0
0
0
0
0
0
SOC
.0
.0
. 3
.4
. 5
.5
.4
.2
.0
.0
.6
.4
SD
0.
0.
0.
0.
0.
0.
0.
0.
0.
o".
0.
0.
SOC
00
04
3
5
4
3
3
4
0
0
4
2
MEAN
0.
0.
1.
0.
2.
1.
5.
4.
3.
2.
21.
14.
SIC
6
6
0
7
1
8
6
2
3
5
7
3
SD SIC
0.2
0. 3
0.9
0.6
1.1
0.50
3.1
1.2
1.9
1.7
22.0
13.7
132
-------
APPENDIX D
Wet/Dry Ratios
Wet/dry ratios are shown for each sample and tissue type
for Experiments I and II. These values may be multiplied by
the metal concentration (yg/g dry weight) values to obtain
concentrations in yg wet weight.
133
-------
Mean Wet/Dry Ratio (x) with n (number of samples) and Standard
deviation(SD) for all organisms sampled in Experiment I and II
Clam
Gut
Shell
Sea
Cucumber
Body
Gut
Muscle
N
12
12
10
10
9
X
10.
1.
4.
11.
9.
S.D.
6
0
7
1
6
1.
0.
0.
3.
0.
80
02
70
40
90
N
13
12
12
13
Not
X
10.
1.
4.
10.
7
1
5
6
determined,
S.
1.
0.
0.
3.
D.
20
00
40
20
combined
with gut
Urchin
Gut
Shell
Snail
Gut
Shell
Oysters
Gut
Shell
Thalassia
Leaves
root/
Rhizomes
"Fouling
organisms"
Sewage
Sediment
Mangroves
Bottom of
hypocotyl
Meristem
above
hypocotyl
Leaves
Roots
10
9
9
9
6
6
9
9
8
2
10
7
7
4
6
15.
2.
4.
1.
9.
1.
10.
7.
5.
14.
1.
3.
7
0
0
0
5
1
2
5
2
8
4
3
3.4
4.5
5.4
3.
0.
1.
0.
1.
0.
1.
2.
2.
7.
0.
0.
0.
1.
60
10
7
0
1
0
90
1
2
10
00
60
,60
.1
1.0
12
13
7
7
12
13
12
13
7
7
13
7
7
6
7
10.
2.
3.
1.
11.
1.
12.
9.
5.
8.
1.
3.
4.
5.
7
0
9
1
6
3
8
9
0
7
4
4
0
3
5.6
2.
0.
1.
0.
2.
0.
2.
2.
1.
4.
0.
0.
30
20
0
0
10
20
90
2
4
1
10
60
0.40
1.
, 3
0.50
134
-------
TECHNICAL REPORT DATA
'I'lease read liiitfuciions on the reverse be/ore row/
l. Ml com NO.
_ EPA-600/3-78r035
4. TITLt AND SUU IITLE
BIOLOGICAL AVAILABILITY OF POLLUTANTS TO MARINE
ORGANISMS
3. RECIPIENT'S ACCESSION-NO.
5. REPORT DATE
March 1978 issuing date_
6. PERFORMING ORGANIZATION CODE
7. AUTHORIS)
John R. Montgomery, Mary Price, John Thurston, Gina
Laite de Castro, Luz Leida Cruz and Domenica deCaro Z
8. PERFORMING ORGANIZATION REPORT NO
unmerman
9. PERFORMING ORG rNIZATION NAME AND ADDRESS
Center for Energy and Environment Research
Mayaguez, Puerto Rico 00708
10. PROGRAM ELEMENT NO.
1BA608
11. CONTRACT/GRANT NO.
IAG-D-4-0541
1?. SPONSORING AGENCY NAME AND ADDRESS
Environmental Research Laboratory
South Ferry Road
Narragansett, R. I. 02882
13. TYPE OF REPORT AND PERIOD COVERED
Final
14. SPONSORING AGENCY CODE
EPA/600/5
16. SUPPLEMENTARY NOTES
1l>. ABSTRACT
This research was initiated to datermine the rates of uptake, by a Thalassia
testudinum ecosystem, of Cd, Cr, Cu, Ni, Pb, and Zn which were leached from sewage
sludge by seawater. The experimental design used aerated flowing seawater (8.4£
Min~*), which passes over a O.l^ bed of sewage sludge before traversing the model
ecosystem. The tanks, both control and experimental, were 9.2mx0.9mxl.lmin
size with a volume of 3.1 x 10^ liters. Each tank contained sand to a depth of 0.5 m
for a total volume of 4.2 m^. The experiment ran for 125 days from March, 1975
to July, 1975 and was duplicated from December 1975 to April 1976.
17.
a.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
Water Pollution
Underwater Environments
Aquatic Animals
b.lDENTIFIF_RS/OPEN ENDED TERMS C. COSATI Field/Group
Metals Uptake from
sewage sludge
57H
I'l. UIUI HUturiON STATEMENT
RELEASE TO PUBLIC
13. SECURITY CLASS (This Report)
UNCLASSIFIED
20. SF.CURITY CLASS (TIlis page)
UNCLASSIFIED
21. NO. OF PAGtS
145
22. PRICE
EPA Form 2220-1 (9-73)
135
*U.S. GOVERNMENT PRINTING OFFICE:1978 260-880/37 1-3
------- |