The Western Environmental
Research Laboratory
U.S. Environmental Protection Agency
Las Vegas, Nevada

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                      FOREWORD

The seal of the  U.  S. Environmental Protection Agency on
the opposite page is particularly appropriate for beginning
the 1971  Annual  Report of  the  Western Environmental
Research Laboratory since this was the first full year that the
Laboratory  was  a  part of  the  new agency.  This report
summarizes the  activities conducted  by the Laboratory in
calendar year  1971  and  reflects  the  transition  from  a
Laboratory devoted almost entirely to problems of environ-
mental radiation  to one concerned with the broader aspects
of environmental pollution. This  transition is perhaps most
obvious in  the acquisition of new aircraft and equipment as
plans  and  proposals were  developed  for remote sensing
projects  and for the National Eutrophication  Survey, to
which this  Laboratory will devote considerable effort during
1972.

All the functions performed by  the  Laboratory cannot be
described in detail  in  a report this  size;  however, certain
topics are discussed in depth and should give the reader an
accurate impression of the  extent of  our  Laboratory's
programs. The report is organized on a functional basis and
hopefully this approach will aid the reader in  gaining the
proper perspective.

We would  welcome at any time inquiries concerning any of
the activities described in this report and  we would like to
express our grateful appreciation to the organizations and
individuals  whose efforts have contributed to the success of
our mission and the attainment of specific objectives.
                           Dr. Melvin W. Carter
                                Director

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the western.     ,
environmental
research
laboratory
For the Western Environmental Research Lab-
oratory (WERL), 1971 was the year 1. Designated
as one of the national facilities of the Environ-
mental Protection Agency (EPA), it shared that
honor with three National Environmental Re-
search Centers (NERC) selected from among the
Agency's forty-four laboratories scattered across
the country.

Established in 1959 as the Southwestern Radio-
logical Health Laboratory of the U. S. Public
Health Service, the Laboratory has concentrated
its efforts on environmental radiation. Late in
1970, when it became a part of the EPA, the
Laboratory was renamed to reflect the broader
areas of interest in which it will participate under
the Office of Research and Monitoring of the
EPA.

In the reorganizations which formed the EPA, the
Laser Section, of the Electronic Products Program
which, under WERL control, was organized and
developed from modest beginnings to a highly
specialized operation, was retained in the U.S.
Public Health Service.

The highlights of the year, our significant ac-
complishments, continue to be drawn from the
environmental radiation programs which received
the major part of the Laboratory's attention.
Although radiation may be a narrow segment of
environmental pollution, WERL activities were
widespread geographically.

From Alaska where well over 500 man days were
devoted to providing radiological safety support
for the nuclear test Cannikin — to Colorado to
determine the environmental effects of the flaring
of gas produced by the nuclear gas stimulation
experiment — Project Rulision —

From Eniwetok in the South Pacific to support
the U.S. Air Force - back to Colorado (and nine
other western states) to determine the extent and
consequences of the use of uranium tailing
material as  fill for dwellings and public facil-
ities.

The world also traveled to Las Vegas on at least
one occasion — for a Tritium Symposium, co-
sponsored  by WERL and the  University of
Nevada, Las Vegas, which had international
attendance.

Meanwhile, at the WERL Experimental Farm,
research projects to assess the metabolism of a
variety of radionuclides by dairy cows continued
to provide data which were relevant not only to
consideration of radiation exposure but have
implications for the behavior of stable elements as
well.

Less dramatic, perhaps, but certainly no less
important was the conduct of the off-site radio-
logical safety program in support of nuclear
testing activities at the Nevada Test Site (NTS).

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AERIAL VIEW OF THE EXPERIMENTAL FARM
                                                       THE EXPERIMENTAL FARM IS OPERATED IN MUCH THE SAME MANNER
                                                                    AS OTHER DAIRY FARMS IN THIS AREA

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The WERL organization chart is presented on the
following page.  However, like most such charts,
what it doesn't show is more important than what
it does show.

It does indicate the three-fold mission of the Lab-
oratory —
   ENVIRONMENTAL SURVEILLANCE

   ...  including monitoring, sample collection
       and community relations

   RESEARCH AND INVESTIGATION

   .. .  including pollutant transport  pheno-
       menology from  source to  man

   TRAINING AND EDUCATION

   ...  including technical assistance and con-
       sultation
.. . And it shows that WERL uses a centralized
service function  for laboratory  analyses, data
processing, electronics, etc. However, it does not
show the lateral interaction among programs; the
use of scientific, administrative, craft and clerical
personnel  on short-term assignments across pro-
gram  lines  to  accommodate the  non-uniform
workload  situation  such as those  for  certain
nuclear tests.

The scope of WERL's administrative authority is
similarly  hidden.  During 1971 the  WERL was
designated as an EPA accounting point to service
not only its own  accounts,  but those of NERC,
Corvallis, Oregon, three Regional Offices and the
National   Field  Investigation  Center,  Denver,
Colorado;  the Laboratory classification authority
was extended to  the GS-15 level to match  its
appointing  authority;  its involvement   in  the
process of  awarding  and administering  research
grants was increased;  and a new Memorandum of
Understanding  between  the  Atomic   Energy
Commission  (AEC)  and  EPA  for  the  Off-Site
Radiological Safety Program  was  negotiated and
is administered by WERL.
      NUMBER OF PERSONNEL

                CIVIL COMMISSIONED
              SERVICE   OFFICER   TOTAL
June 30, 1962      65

June 30, 1963      82

June 30, 1964*    102

June 30, 1965*    141

June 30, 1966*    176

June 30, 1967*    204

June 30, 1968*    222

June 30, 1969     189

June 30, 1970*    183

June 30, 1971*    167

December, 1971   173

*lncludes summer employees.
25
28
40
49
64
78
75
74

64
59

47
 90
110
142
190
240
282
297
263
247
226
220
 TOTAL BUDGET BY FISCAL YEAR
              (Thousands)
Fiscal
Year
1963
1964
1965
1966
1967
1968
1969
1970
1971
1972
DHEW
R
643.6
1,020.7
659.3
593.5
924.2
1,184.5
1,261.0
1,229.0
213.9
0
AEC
eimbursat
557.6
1,020.3
1 ,624.7
1,786.3
2,068.3
2,277.2
2,257.0
2,271.0
2,229.0
2,367.0
                         EPA
                        1,131.3
                        1,600.1
      TOTAL


      1,201.2
      2,041.0
      2,284.0
      2,379.8
      2,982.5
      3,461.7
      3,518.0
      3,500.0
      3,574.2
      3,967.1

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                            ORGANIZATION CHART
          WESTERN ENVIRONMENTAL RESEARCH LABORATORY
MARIANNE CARPENTER,
 SECURITY OFFICER
MELVIN W. CARTER
   DIRECTOR
JOHN R. McBRIDE,
   DEPUTY
   DIRECTOR
                                                                   ASSISTANT TO THE DIRECTOR
                                   D. E. BERNHARDT

- 1
1








1 OFFICE OF DOSE ASSESSMENT
AND SYSTEMS ANALYSIS
F. N. BUCK, CHIEF

OFFICE OF
RADIATION SAFETY
J. S. COOGAN, CHIEF

OFFICE OF
TECHNICAL REPORTS
R. D. TATE, CHIEF

^^»









•^^










OFFICE OF
ADMINISTRATIVE SERVICES
NORMA COX,
MANAGEMENT OFFICER

OFFICE OF
QUALITY CONTROL SERVICES
R. F. SMIECINSKI. CHIEF

OFFICE OF
INFORMATION
G. S. DOUGLAS, CHIEF











TECHNICAL TRAINING
T. C. SELL, CHIEF


ENVIRONMENTAL SURVEILLANCE
D. T. WRUBLE, CHEF


RADIOLOGICAL RESEARCH
A. A. MOGHISSI, CHIEF


TECHNICAL SERVICES
R. E JAQUSK CHIEF

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                       TYPES OF POSITIONS AT WERL
ACCOUNTING TECHNICIAN
ADMINISTRATIVE OFFICER
AGRONOMIST
AIRCRAFT OVERHAUL INSPECTOR
AIRPLANE PILOT
ANIMAL HUSBANDMAN
BIOLOGICAL AID
BIOLOGICAL TECHNICIAN
BIOLOGIST
BOTANIST
CARD PUNCH OPERATOR
CARPENTRY WORKER
CHEMIST
CHIEF, ENVIRONMENTAL SURVEILLANCE PROGRAM
CHIEF, OFFICE OF INFORMATION
CHIEF, OFFICE OF QUALITY CONTROL SERVICES
CHIEF, RADIOLOGICAL RESEARCH PROGRAM
CHIEF, TECHNICAL SERVICES PROGRAM
CHIEF, TECHNICAL TRAINING PROGRAM
CLERK
COMPUTER  OPERATOR
COMPUTER  SYSTEMS ANALYST/PROGRAMMER
CROP AND LIVESTOCK RESEARCH LEADER
CROP AND LIVESTOCK RESEARCH HELPER
DIRECTOR
ELECTRONICS ENGINEER
ELECTRONICS TECHNICIAN
ENGINEERING  TECHNICIAN
EQUIPMENT FABRICATOR
EQUIPMENT REPAIRER
FISCAL TECHNICIAN
GENERAL PHYSICAL SCIENTIST
HEALTH PHYSICIST
ILLUSTRATOR
INSTRUCTOR
JANITOR
LABORATORY ANIMAL RESEARCH WORKER
LABORATORY ANIMAL RESEARCH HELPER
LIBRARY TECHNICIAN
LIGHT VEHICLE OPERATOR
MACHINIST
MATHEMATICAL AID
MEDICAL OFFICER
METEOROLOGIST
OFFICE MACHINE OPERATOR
PATHOLOGY  TECHNICIAN
PERSONNEL MANAGEMENT SPECIALIST
PHYSICAL SCIENCE AID
PHYSICAL SCIENCE TECHNICIAN
PHYSICIST
PHOTOGRAPHER
PLANT PHYSIOLOGIST
PURCHASING  AGENT
RESEARCH CHEMIST
RESEARCH PHYSICIST
RESEARCH RADIOBIOLOGIST
SECRETARY
SECURITY SPECIALIST
SOIL SCIENTIST
STAFF ENGINEER
STAFF SANITARIAN
STATISTICIAN
STOCK KEEPER
SUPPLY MANAGEMENT OFFICER
TECHNICAL PUBLICATION WRITER-EDITOR
TELEPHONE  OPERATOR
TRAINING TECHNICIAN
VOUCHER EXAMINER
WAREHOUSEMAN
WORKER-TRAINEE

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Committees and  other review  panels  serve  in-
directly as quality  assurance aids to  administra-
tion and management. An Equipment Committee
reviews all  capital  equipment  purchases.  Pro-
fessional representatives of each  of  the  WERL
programs  function  as  the  Scientific   Review
Committee  to  screen  all  WERL-generated  ab-
stracts, papers  and technical  reports. Extensive
health  physics  and  quality control  coverage  is
provided for many of  the  research  and  experi-
mental projects conducted by the WERL. Further
protection of WERL employees and equipment as
well as that of  the  general  public  is pursued
through a variety of industrial  safety activities,
including regular and  non-routine inspections and
short-term safety courses.
SECURITY

A total protective  program is  provided  through
the services of the  Security Office.  A  security
staff coordinates  the use, storage, and transit of
information and data of a classified nature. The
Security  Office also  provides for property safe-
guard,   using  security  guards  to  ensure  that
property and equipment are adequately secured
during  non-duty hours, and to control  access to
the six WERL buildings to  employees, University
students and other  authorized  persons.  WERL
employees  serve as Duty Officers who expedite
corrective  action  in  times of after-hour  emer-
gencies  and acknowledge inquiries received from
the public.  Through orientation, an awareness for
individual  responsibility for  the  protection  of
Government  property is  encouraged  in  WERL
employees. The WERL Security Office received
additional  responsibilities during 1971  from the
Security &  Inspection  Division,  EPA. In this
regard,  the WERL will assist that  Division  in
conducting  physical  security surveys  and per-
sonnel  security interviews  for the EPA Regions.

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8
MEDICAL

The WERL  Medical  Officer, in  his  capacity as
medical advisor to the Director, is responsible for
investigating  the medical aspects of  alleged off-
site  radiation exposure  as a result of testing
activities  at  the  NTS. Also, the Medical Officer
serves as the Coordinator of the Medical Liaison
Officer Network (MLON)  whose purpose  is to
provide physicians in locations other than Nevada
to  investigage radiation  injuries which  are  re-
portedly  due to nuclear testing activities con-
ducted by the AEC. Representing every state, the
District of  Columbia and  Puerto Rico, MLON
membership  is  composed  of physicians knowl-
edgeable in the field of radiation bio-effects and
radiation  injury- Credentials are reviewed  and
appointments made  by the Coordinator whose
duty  is to  maintain files  of case  records and
perform   liaison  with the  AEC. Timely infor-
mation on  the possible methods of preventing
injury and the diagnosis and treatment of radia-
tion injury is compiled and disseminated by the
Coordinator  in the MLON  Bulletin and periodic
training conferences  are held in  Las Vegas.  At
these meetings, the basic aspects of radiobiology
and radiation injury are reviewed, new concepts
in the area of radiation effects are discussed and
special features  of  recent  AEC nuclear testing
activities which could lead to claims of radiation
injury are evaluated.  In addition, the Medical
Officer in the spring of 1971 performed complete
physical  examinations including  complete blood
counts and thyroid profiles on 142 persons living
in the vicinity of the Nevada Test Site. Medical
examinations  revealed  a   generally  healthy
population group. The  Medical  Officer also pro-
vides  emergency medical  treatment  of job  in-
curred injuries, palliative treatment of illness and
other general medical services.


QUALITY CONTROL

The Office of Quality Control provides services to
the various  laboratory  programs to ensure the
reliability of data produced  at the  WERL. Re-
sponsible  for assisting  in  WERL programs and
those of  other state and federal laboratories in
compiling and reporting  radiological data within
acceptable  limits for accuracy and precision, this
office prepares and submits radioactive standards
for the.  calibration  of  analytical  systems and
maintains close  scrutiny of analytical results to
recommend corrective action when necessary.

Cross-check samples are submitted  monthly to
participating   laboratories   to  document  the
validity  of their  analytical  methods.  Quality
control reports are prepared monthly and include
tables and charts designed to indicate clearly any
trends in laboratory data, particularly adverse or
unanticipated  trends. On request, isotopes are
calibrated  against primary  standards generally
within a two standard deviation error of + 10%. A
radioactive standards' stockpile and monthly in-
ventory  listing  are  maintained for  use  by all
Cross-check Study participants.

Additional  responsibility assumed during  1971  in
conducting cross-check  programs resulted in the
development   of  new  techniques to  improve
efficiency.  To ensure that the samples contained
the known amounts of the radioactive standards,
cross-check  samples  were   prepared in  large
batches to minimize sample non-uniformity; all
primary and secondary standards were rechecked
for accuracy and all samples were counted on the
WERL systems  as a last check of their  proper
makeup.

During 1971, the WERL participated in numerous
cross-check studies with many organizations in-
cluding  the   World  Health  Organization, the
Analytical  Quality Control  Service, the  Inter-
national Atomic  Energy Agency and others.

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                                                                9
           QUALITY CONTROL CROSS-CHECK ACTIVITIES
WERL CROSS-CHECK NUMBER OF PARTICIPATING NUMBER OF
PROGRAM NAME LABORATORIES SAMPLES PREPARED
Plutonium
in Soil
States Assistance Milk
Tritium in
Water and Urine
1970
7
5
3
1971
12
8
5
1970
10
30
36
1971
50
96
60
NUMBER OF
REPORTS WRITTEN
1970
0
6
12
1971
3
12
12
TOTALS
15
25
76
206
18
27
             QUALITY CONTROL COMPUTER PROGRAMS
PROGRAM NAME
Inventory of Radioactive Standards
Monthly Report of OQC Samples Prepared
Daily Radioactive Standard Usage Log
States Assistance Milk Cross-check Program
Plutonium in Soil Cross-check Program
Tritium in Water and Urine Cross-check Program
PMN Duplicate Milk Analysis
FREQUENCY
Monthly
Monthly
Monthly
Monthly
Monthly
Monthly
Bi-monthly
NUMBER OF COPIES
DISTRIBUTED
18
6
6
8
15
5
6
                                        TOTAL
                                 64

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10
              WERL CROSS-CHECK PROGRAM PERFORMANCE FOR 1971
      CROSS-CHECK PROGRAM
       AQCS Milk
         I t    II
         II    II
       AQCS Water
           90
           89
Sr
                   140
                   137
           90
           89
I
Ba
K
Cs
Sr
Sr
         i i    ii
         11    r~*
       AQCS Diet
      Gross a
      Gross £
      Radium
      Tritium

           90
           89
          131,
          140
          137,
'Sr
'Sr
II   II
       WERL Milk
         !»   I »
       WERL H20
       WERL Urine
                      'Ba
                      Cs
                      K
                    89
                   131
                   140
                   137
            'Sr
            I
            'Ba
            K
            Cs
J






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                     Note: "wf" indicates no analysis performed.
                          "W" indicates analysis is within ±3 sigma Control Limit.
                          "O" indicates analysis is outside ±3 sigma Control Limit.

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                                                                                               11
RADIATION SAFETY

The extensive use of radioactive materials at the
WERL  required  that  strict  and  continuous
radiation safety surveillance be maintained. Close
coordination  and cooperation  with  each  user
facilitated the safe and uneventful handling and
storage  of  many separate isotope solutions and
sealed sources, including the following:
  • Sixty-two radioisotope shipments received
    at the WERL.

  • Six hundred and six cubic feet of contam-
    inated  waste transported  to the  Nevada
    Test Site for disposal.

  • Three   hundred  and   sixty-three  urine
    samples submitted for  laboratory bioassay
    analysis. These samples were collected from
    WERL personnel working with  luminous
    compounds  and  from  WERL  personnel
    working on  various experiments involving
    radioactive materials.
In addition  to  providing routine health physics
coverage for laboratory  experiments, all sealed
sources  one millicurie  or larger were physically
inspected  and  swipe-tested  for possible source
leakage. All were found to  meet current safety
requirements,  specially  AEC  regulations  under
Title 10 (CFR).

Indicative of  radiation  safety coverage during
1971, the WERL was  inspected for compliance
under its Byproduct Materials License and Special
Nuclear  Materials  License by the  Division  of
Compliance,  AEC,  and  found  satisfactory.  In
addition to the WERL name change, amendments
to the  licenses included authorization to the
WERL   to   handle   increased  amounts  of
plutonium,  i.e., eighty  grams encapsulated  as
Pu-Be neutron  source and five milligrams in any
physical or chemical form.

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dose
assessment
 The function of the Office of Dose Assessment
 and Systems Analysis is primarily to evaluate the
 impact on the environment of nuclear testing and
 other radiological activities, particularly in terms
 of the health and safety of the general  public. In
 addition to performing calculations of  exposures
 to people based on environmental sampling and
 monitoring, this office also maintains the whole-
 body counting and  scanning  facilities at the
 WERL to locate, identify and  measure gamma-
 emitting radioactive materials deposited within
 the body as a result of inhalation and/or in-
 gestion.

 A significant  project conducted during  1971
 included preparation of a summary tabulation of
 hypothetical  thyroid doses  which might have
 accrued to an individual in the off-site area as a
 result of inhaling radioactive  effluent, and/or
 ingesting  milk contaminated with radioiodine
 attributable  to tests at the Nevada Test Site or
 foreign nuclear tests from  1963 through the
 present. The  "critical receptor" used for all
 calculations  was a hypothetical infant, having a
 two-gram thyroid and ingesting one liter of milk
 per day and inhaling 4.7 m3 of air per day. An
 attempt was made to incorporate all environ-
 mental data available at the WERL in performing
 these calculations. The results shown in the table
 are conservative because infants were not present
 at most locations, and no allowance was made for
 the fact that collection of a sample often involved
       the exchange of uncontaminated milk for that
       produced at a given  location, and the highest
       concentrations were carried forward or backward
       as necessary to fill in days on which samples were
       not collected.

       Also during 1971, a method was developed for
       the extrapolation of  residual external gamma
       exposure rates to obtain more accurate estimates
       of hypothetical infinity exposures. The method
       entailed use  of the  isotopic composition of
       deposited gamma-emitting activity as determined
       from environmental samples to project the decay
       of the gamma field. Previous practice had been to
       assume decay as a power function of time (t-1-2)
       which generally resulted in disagreement between
       thermoluminescent  dosimeter (TLD)  measure-
       ments and estimates of the hypothetical infinity
       exposure from survey meter readings. By com-
       bining the new extrapolation technique with plots
       of exposure  rate,  TLD measurements can be
       extrapolated to obtain hypothetical infinity ex-
       posures which agree with those calculated from
       survey  meter readings within the measurement
       accuracy of the  two  types of instruments. The
       method was applied to external gamma dosimetry
       for the Baneberry Event, an underground nuclear
       test which vented radioactivity to the atmosphere
       in December 1970.
              HIGHEST THYROID DOSES TO A HYPOTHETICAL INFANT
                   (Attributable to NTS Nuclear Activities 1963 - 1971)
       LOCATION

       Near Eureka, Nevada

       Near Grangeville, Idaho

       St George, Utah


       St George, Utah
        TIME

        1965

        1965

        1963
   (from foreign testing)

        1965
(from a combination of sources)
CALCULATED DOSE

    1.5 rod

    0.2 rad

    10 mrad


    10 mrad

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                                                                                                 13
WHOLE-BODY COUNTING

The physical installation for whole-body counting
at the WERL  consists of two adjacent chambers
each nine feet by nine feet by fourteen feet, with
walls, floors and ceilings  of steel five inches thick
for shielding  of  the detectors.  For qualitative
identification  as  well  as quantitation  of body
radioactivity, the detector used is a sodium iodide
crystal eleven  inches in diameter and four inches
thick   coupled,  through  seven photomultiplier
tubes, to  a  pulse-height  analysis system.  For
determination  of spatial distribution  of body
radioactivity, a two-dimensional scan is perform-
ed with  a smaller detector also coupled to the
pulse-height system.

-------
14
From data obtained, the resulting dose can be
calculated. Since the  whole-body  counter does
not  provide  an absolute  measurement of the
amount of radioactivity in the person, calibration
of the system is performed by using a  phantom
which is  designed  to simulate actual persons in
size and shape.

This system  is being used in a special  study to
determine the body burdens of residents in the
area surrounding the NTS.

Results from the  initial data collected  on 142
persons in the spring of 1971 demonstrated that
the  cesium-137  burdens  displayed a  normal
distribution, apparently unaffected by azimuth or
distance from the NTS, with a mean of 25 pCi/kg
of body  weight. Tritium  concentrations deter-
mined from  urine samples collected from these
individuals showed a  slightly  different disbri-
bution  pattern with several values being clearly
above the normal  distribution  function. It was
determined that these elevated  levels were attri-
butable   to  exposure  to  leaking tritium-dial
watches.

           A COMPARISON OF
       3H BURDENS AND DOSES
    FOR TWO POPULATION GROUPS
    510=
    102
              NEVADA *
              ATKA—•
                    50
                 PROBABILITY (X)
           BURDEN AND
      THEORETICAL DOSE TO
 RED BONE MARROW DUE TO 55Fe
o30
z
025
520
8«
 10
                                    o
                                    o
                                    o
                                    03
                                    O
                                    z
0.2


  z
  3
                                  0.1
          10
                  50
              PROBABILITY (%)
        A COMPARISON OF
 THEORETICAL DOSES TO THREE
POPULATION GROUPS DUE TO ?37Cs
 102
                                                    10'
                                                    10°
                                                    10
                                                            ATKA
                                                            FALL 1971
                                                            	.*••••""
                                                            N.T.S. (OFF SITE)
                                                            SPRING 1971
           10       50       90
               PROBABILITY (%)
                                   99

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                                                                                                15
Off-site  whole-body   counting   projects  were
continued during  1971,  utilizing compact port-
able counting  equipment designed for expedient
and  safe handling and transportation to remote
areas such as the small inland villages of Alaska.
The program to measure cesium-137  burdens in
Eskimos involved two surveillance trips to Alaska
during  1971. To compile additional  information
on  the  seasonal variation of cesium-137 and  to
detect  long-term  trends,  Noatak and Kiana were
visited  in the  spring and again in the fall, and a
trip to Shishmaref was conducted in late fall.
Surveillance of human burdens was also perform-
ed on the  island of Atka in connection with the
Cannikin Event. All residents above the age of six
were  whole-body  counted   to  determine
cesium-137 burdens. In addition, blood and urine
samples were obtained to determine iron-55 and
tritium  levels, respectively. Tritium levels were
found to be almost identical with those found in
the NTS  off-site population. Iron-55 levels agreed
with values observed by other investigators study-
ing similar population groups.
                                                                  INTERIOR OF THE WERL WHOLE-BODY COUNTER

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programs
LABORATORY OPERATIONS

The chemistry and counting laboratories at the
WERL provide continuing analytical support for
current programs. Laboratory capabilities include
routine analysis for samples collected through the
established surveillance networks, both AEC and
EPA, as well as quick response during periods of
accelerated sampling in connection with nuclear
testing activities and to special requests for
analytical assistance or bench training from states,
regional offices and other government agencies.

Both radiochemical and stable chemical analyses
are performed; over 7,000 separate chemical
analyses  were performed by  the  chemistry
laboratory in  1971. This figure does not include
standards or special  method  development
samples.

Over  18,000  environmental samples  were
analyzed  for gamma-emitting radionuclides and
600 calibration standards were gamma-counted
utilizing 4- by 4-inch Nal (Tl) crystals and gamma
spectrometers. Alpha and beta counts made on
end-window gas-flow proportional counters total-
ed 89,585.

During 1971, the plutonium analysis of environ-
mental samples was placed on a routine basis. The
procedure utilizes  multiplace ion  exchange
column holders, electroplating cells and counters.

MULTI-PLACE ELECTROPLATING UNIT FOR PLUTONIUM ANALYSIS •+-
                       TYPES OF SAMPLE ANALYZED
    PRINCIPAL
              PF&
                                ANIMAL  GAS &
ANALYSES CC MILK
Radio- strontium 770
Gross Aipha-Beta 29,386*
226RQ by emanation
Radio-krypton
Radio-xenon
Tritium 163
14C
Uranium
Plutonium
Stable calcium
Selenium
X-Ray scans
Thorium
lron-55
Gamma 11,650 1,808
FOOD WATER TISSUE
60 44 69
1,163
39 1


141 1,270 46

10
1 20 223
67 48




252 1,216 613
AIR
11


161
40
510
11

296





1
SOIL BIOASSAY DARDS MISC.
49
263
12


167 901 37

22
445 77

3
40
4
53
464 56 600 2,452
TOTAL
1,003
30,812
52
161
40
3,235
11
32
1,062
115
3
40
4
53
19,112
    'Represents 88,159 total beta counts

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17

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18
Four  more alpha counting systems were built to
bring  the  total  capability  to eight alpha spectro-
meters.

The plutonium methodology was improved such
that 10-gram aliquots of tissue ash and 100 grams
of bone ash can now be analyzed. Improvements
in the precision and economy of the plutonium-
in-soil  method  were realized.  The uranium and
thorium  methods were revised to provide for
analyses by ion exchange and alpha spectroscopy
as well as by the routine methods.

The   metal  gas  analysis  system  pictured  was
designed and constructed by the WERL in 1971.
This system  is  used for radionuclide analysis of
noble  gases found  in   air,  e.g.,  krypton-85,
xenon-133, and argon-37 and -39.
Other innovations during 1971 include a method
for determining stable selenium in soil, vegetation
and  water  by chemical  separation and  x-ray
fluorescence. This method is sensitive  to about
0.1 micrograms. Methods were also developed for
determining iron-55 and  stable  iron  in blood
samples. The minimum  sensitivity was shown to
be about 600  pCi/l  of  whole blood for iron-55
and 0.002  milligrams for the stable iron deter-
mination.  The method was developed for appli-
cation to analysis of blood samples from Alaskan
natives.
GAS ANALYSIS SYSTEM
                     ALPHA SPECTROMETRY SYSTEM-

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19

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20
                                                  COMPUTER OPERATIONS

                                                  WERL computer  capabilities utilize the central
                                                  computer complex  of the AEC by direct  con-
                                                  nection through a CDC Batch Processing terminal
                                                  maintained by the WERL. The central complex
                                                  consists of a CDC 6400.

                                                  The computing workload over calendar year 1971
                                                  was evenly split between development and  pro-
                                                  duction; 30.3 hours of  Central  Processing  Unit
                                                  (CPU) time being  applied in production jobs and
                                                  31 hours utilized for program development. Total
                                                  utilization of the 6400 system since initial hook-
                                                  up in October 1970 is shown below.
                                  COMPUTER UTILIZATION
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-------
                                                                                                         21
       COMPUTER UTILIZATION (CENTRAL PROCESSOR  SYSTEM HOURS)
                                           DATA PROCESSING  SYSTEM
    OPERATING
    PROGRAM
TYPE
 OF
RUN
MISC.
SYST.  DATA
DEV. MANGT.  FISCAL
                                         MILK
                            AIR  GAMMA  DIR.   DOSIM.  CHEM.
       SUB
BIOG.  TOTAL  TOTAL
Program Direction
      .650   .498    .263  1.544
     2.634   .198    .104   .247
                                                          .044
                                                 .003
                                                                              .003
                                                             3.002
                                                             3.186   6.188
Electronic Products
      .138
     2.279
                                                             .582    .720
                                                                    2.279   2.999
Notional Surv. Nets.
      .822
      .078
                               .002
               .803
               .155
                           1.799
                            .002
                                                                              .129
       3.552   3.790
        .238
Radiation Research
      1.501
      .959
                                      .204
                                   .629
                                               .008
                               .002
       2.343
        .960   3.303
AEC Offsite
 P    .002   .047   4.380         6.874   2.086  1.236    .513  .280
 D    .375  2.036   3.886          .233   .364  6.884   10.281  .063
                                                             .072  15.489
                                                             .039  24.161  39.650
Indoor Radon
 P
 D
                        .003
              5.109
               .198
                                          .099
                                          .007  .001
       5.211
        .206   5.418
Sub Total
 P   3.115   .544  10.759  1.544
 D   6.325  2.238   4.344   .247
                           6.874   4.558   1.236    .615  .417   .654  30.320
                            .233    .367   6.884  10.288  .067   .039  31.030
        TOTAL     T   9.440   2.782  15.103  1.791
                   P = Production
                   D ~ Development
                                 7.107  4.925  8.120   10.903  .484    .693   61.350  61.350

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22
SYSTEMS ANALYSIS AND PROGRAMMING

Of the  45  requests  for  systems  analysis  and
programming support  received during  1971, 34
were completed and resulted in the conversion or
modification of 36 programs and the creation of
18 new programs. A comprehensive data manage-
ment system  for dosimetry data was  initiated
during  1971 and will  have been completed at
publication of this report.

A  management  information system  was  also
developed in support of the Uranium Mill Tailings
Project.  This  system  will  provide  a  running
account of the  status and  results of the screening
surveys  of  the  Indoor   Radon  Study.  Con-
currently,  WERL historical environmental  sur-
veillance data files are being converted  from a
card-oriented system  to a mass storage-oriented
system.

ENGINEERING SERVICES

The broad scope and great variety of WERL field
and  laboratory  programs  indicate  a need  for
timely support  capabilities in design, fabrication,
maintenance and repair. Accordingly, the WERL
maintains in-house plant  facilities  manned  and
equipped to provide the required engineering and
craft services.

The WERL  utilizes  more  than three  million
dollars worth of  instrumentation  including com-
munications  and  remote  sensing  devices.  To
ensure optimum  operating usage and minimum
downtime, electronics  and electrical capabilities
are maintained  in the  repair, maintenance  and
modification of all electronic equipment, calibra-
tion and standardization of  instruments, and the
development and assembly of new systems.

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                                                                                                    23
  MAJOR ELECTRONICS PROJECTS
                    1971

 1.  Environmental  growth chambers were  re-
    wired for  flexibility,  ease  of  operation
    and  safety.

 2.  A  Wide Beta gas  saver system was de-
    signed, fabricated and installed.

 3.  Several different types  of detectors were
    evaluated for use in the LSI field monitors.

 4.  A  solid state alpha  counting system was
    designed  and  put  together  with modular
    components.

 5.  A  twelve position  electro-plater unit was
    designed, fabricated and made operational.

 6.  A  second digital  log-rate meter was con-
    structed.

 7.  A  special  gamma  counting  system was
    designed  for use in an automobile  in con-
    nection with the Indoor Radon Study.

 8.  A  data acquisition system was designed
    and fabricated for use in the aircraft.

 9.  Electrical   controls for  the  cage  washer
    were modified.   Safety  devices were  in-
    stalled to  help alleviate operator errors.

10.  A  Mercury  Vapor  detection  system was
    fabricated.
      MAJOR CRAFTS PROJECTS
                    1971

 1.  A high temperature Lithium Reaction Cham-
    ber was designed and fabricated.

 2.  Calibration  well mechanized components
    were  fabricated.

 3.  Camera  mounts for multiple camera  use
    were  designed and constructed.

 4.  Trays for off-site vehicles were  fabricated
    and  installed.

 5.  An all-metal  gas  analysis  rig  was built.

 6.  A liquid nitrogen  dewar filler assembly
    was fabricated for the solid  state detector.
                      «
 7.  A trophy case was designed and built to
    display trophies won by WERL competitors.

 8.  Tow bars were fabricated for WERL aircraft.

 9.  Plexiglas safety covers were made for the
    vacuum chambers.

10.  A natural gas  burner was  designed  and
    constructed for collection of water vapor
    for tritium analysis.

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24
Continual  physical plant upkeep, corrective and
preventive maintenance and  repair of real  pro-
perty and equipment, rehabilitation and  modifi-
cation  of office  and  laboratory  spaces,  and
industrial safety support are provided through the
Engineering  Section.  Comprised  of highly  pro-
ductive shops  specializing in air  sampler repair,
carpentry, painting,  machine work,  sheet metal
and  welding, this  group concurrently maintains
support  liaison with  the building  and  grounds
staff of the University of Nevada, Las Vegas, and
with local general contractors.

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                                                                                                25

RADIO STATION WA7AEL

At 6:30 a.m., February 9, 1971, an earthquake
rocked  Los Angeles. All communications in the
affected  area  were  lost,  including  the wire
services, and switchboards in the vicinity were
swamped with calls. The American Red Cross, Las
Vegas Chapter, called on the WERL for help.

A  standby  radio station  is  maintained by the
WERL  to  provide immediate comrrunications
support for  radiological safety activities. Using
call letters WA7AEL  and operated by  a  WERL
electronics technician, this facility also  serves in
the DHEW Emergency Communications System.
Accordingly, the WERL offers radio communi-
cations  services  in time  of  disaster  or other
emergency,  including  the coordination  of emer-
gency  information  and  news exchange, relay  of
messages,  arrangement for supplies and authori-
zations  in  and out of disaster areas, and operation
of a radio net control service.

An hour after the Los Angeles earthquake struck,
WERL  radio made initial contact with  mobile
units  in the Sylmar and other affected areas and
established  net  control. WA7AEL arranged to
have the news media phone-patched to a mobile
unit and provided critical assistance in preparing
an evacuation plan concerning an impending dam
burst by  obtaining and  relaying information to
the cognizant authorities. At this time also, the
station  was the only means of communication
available to the  Red Cross whose Los Angeles
Chapter received over a quarter million telephone
calls and  the Las Vegas  Chapter which received
over 300.

Other  net  activities included  support  of the
Pacific  Atoll Cratering  Experiment program  at
Eniwetok   in   the  Marshall   Islands  and  the
Cannikin  Event  in  Alaska, and a  life-saving re-
sponse  to  a very weak distress call  from the
jungles  of  Guatemala. The WERL  radioman ar-
ranged  to  have  badly-needed drugs transported
from Colorado  to the surgeon  in the wilderness
and, the patient  survived. A letter of appreciation
from the doctor added to the many citations and
awards bestowed on  the WERL station which, in
1971,  also  included the American  Red  Cross
Disaster Trophy and the  West Coast Amateur
Radio Service Award.

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environmental,
moniTormg and
surveillance
The WERL maintains continual radiological sur-
veillance to detect and document radioactivity,
regardless of its origin, in any locale within its
area of responsibility, particularly in the areas
around the site of nuclear testing activities spon-
sored by the U.S. This surveillance includes
personnel and field dosimetry, monitoring with
radiation measuring instruments and sampling of
air, water, milk, soil, vegetation and animal tissue.
An immediate-action readiness is maintained to
protect the population from exposure to environ-
mental radiation. These monitoring and surveill-
ance services are provided through a two-part
program, one phase oriented specifically toward
the individual and the second to ascertain the
exposure of a large segment of the population:
  » To assure the radiation safety of the public
  living in proximity to the site of any
  nuclear test conducted by the U.S., one
  phase employs a dosimetry system to
  measure individual or area exposures, and
  mobile monitors equipped with  suitable
  instruments to measure exposure rates.
  This phase also incorporates a capability
  for rapid response to an emergency radia-
  tion situation.

  'To obtain estimates of the dose equivalent
  from radiation levels attributable to nuclear
  activities, whether underground or excava-
  tion projects, rocket engine tests or other
  sources including worldwide or nationwide
  fallout, routine sampling of the environ-
  ment at fixed locations is pursued through
  established sampling networks.
GROUND SURVEILLANCE

For every nuclear event under WERL surveillance
responsibility,  mobile monitors are deployed
along the downwind trajectory, which is pro-
jected in terms of release potential and possible
magnitude. Reserve  monitors  standby at the
Laboratory for prompt dispatch to strategic
locations when a release of activity is reported.
When ground motion is anticipated, additional
monitors are stationed at mines, structures, and
certain other locations to advise people of the
event schedule and provide safety assistance
including, if necessary, aid in evacuating specific
structures designated by the AEC.

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                                                                              27
      VARIOUS SURVEILLANCE AND SAMPLING LOCATIONS
        MEXICO N




AR SURVEILLANCE NETWORK STATIONS
ROUTINE MILK SAMPLJNG STATIONS
                                                 ROUTINE DOSIMETRY STATIONS
ROUTINE WATER SAMPLING STATIONS

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28
                                                      A two-way radio system is utilized for the timely
                                                      deployment  of   monitoring   and  surveillance
                                                      personnel and for the interchange of information
                                                      and  instrument  readings to facilitate optimum
                                                      surveillance coverage. Other equipment furnished
                                                      to each monitor includes portable survey instru-
                                                      ments  with a wide range of measurement  ver-
                                                      satility  and  portable,  battery-operated   air
                                                      samplers which are  capable of  operating for at
                                                      least  12 hours  to  collect  particulates, various
                                                      gaseous  elements,  and atmospheric  moisture.
                                                      These  air samplers  use a four-inch  glass fiber
                                                      paniculate  filter and a 3-1/2- by 1-inch  activated
                                                      charcoal cartridge  or a  molecular sieve cannister.
                                                      Portable  gamma rate recorders, consisting  of a
                                                      Geiger-Mueller survey instrument connected to a
                                                      strip chart recorder, are set  up  independently
                                                      allowing  the  monitor a greater mobility. These
                                                      units also  contain TLD's  to  measure the total
                                                      exposure at the location. Monitors distribute an
                                                      additional  number of  TLD's to  persons  in an
                                                      effluent  trajectory  area  who  are not  wearing
                                                      dosimeters as part of the routine network.

                                                      A variety of containers  and  collection equipment
                                                      is  provided for  milk, water, soil and vegetation
                                                      samples.  For special  events the monitors set up
                                                      and  operate  specialized equipment,  including
                                                      atmospheric moisture samplers.
                                                      BATTERY-OPERATED AIR SAMPLER

                                                      MONITORING EQUIPMENT TYPICALLY CARRIED BY WERL MONITORS

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                                                                 29
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LASTIC BAGS
  , TAGS
 PORTABLE
^RECORDER
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.BATTERY POWERED
    AIR SAMPLER
                                        INSTRUMENTS
                                        500 B C2)
                                     RADECTOR
                                                     CD

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30
AERIAL SURVEILLANCE

The broadened scope of responsibilities, assigned
and anticipated  in terms  of  EPA  objectives,
launched  a new era in WERL aircraft  services
during  1971.  Additional aircraft were acquired
and remote sensing capabilities were developed,
including  highly  specialized ancillary equipment
and instrumentation.

The WERL airfleet, upgraded  and expanded in
1971, includes:
   Two  twin-engine  Turbo-Beech  aircraft
   which are used primarily in cloud-tracking
   and sampling in support of nuclear testing
   programs of the AEC. These planes are also
   used for rapid transportation of personnel
   and  equipment and  timely pickup and
   delivery of samples for analysis.

   One twin-engine C-45H which  is used for
   special missions not related to AEC nuclear
   testing  and  to provide service  similar  to
   that of the Turbo-Beeches.

   During  1971, the C-45H  was  used  as a
   primary tracking aircraft  in a pilot  study
   and  survey  of radioactive effluents from
   the Humboldt  Bay Nuclear Power Plant.
   Based on these studies, plans have been
   developed to survey nuclear power plants
   more intensively and a C-123B aircraft was
   obtained  to  provide  tracking  capability.
   The C-45H was used similarly in support of
   two  high  explosives  effects   tests  in
   Colorado  and one in Nevada which were
   conducted by the Defense Nuclear Agency.
   In this service, special on-board equipment
   was  used to  track  the  dust  cloud and
   collect  samples  of dust  and tracers  for
   laboratory analysis. The  C-45H  was also
   used to  photograph the beach and off-shore
   areas  following  the San  Clemente, Cali-
   fornia, oil spill.
A C-123B which is a twin-engine cargo plane
with a much  larger capacity and range than
the C-45H, capable of transporting portable
laboratory  equipment to distant locations
for rapid  survey and assessment of radio-
active materials.

The C-123B is equipped with detection and
sampling gear designed  and constructed at
the WERL and  which incorporates design
features included in the Turbo-Beech  air-
craft,  plus  multi-channel, pulse height
analysis  for  identifying  specific  radio-
nuclides in an effluent cloud. The  plane is
also equipped  with  camera mounts  for
aerial photography.

One T-34B, a single engine plane equipped
for radiation  tracking,  which is used  in
support of the Turbo-Beech missions.

One OV-1C  Grumman  Mohawk, which is
especially   capable  of  performing  aerial
photography,  multi-spectral scanning and
side-looking  airborne   radar surveillance.
Used  extensively by the  U.S.  Army  in
reconnaissance,  the  plane is  particularly
well suited to remote sensing of environ-
mental pollutants.

One TH-55 Hellicopter which  is used to
provide pilot  proficiency training and  for
low level  radiation  monitoring. This two-
man craft, acquired late in  1971, is used to
develop special techniques for application
in  heavier-duty  replacement helicopters
which are on  order.

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                                                                         31
                  EPA
                  AEC
WERL S TURBO-BEECH AIRCRAFT

     TOTAL FLIGHT TIME PER YEAR
     900
     800
   •£ 700
   h.
   ° 600
   UJ 500
   ^ 400
   l-
   J 300
   ^ 200
     100
        0
CD  CD  CD  tO  CD CD
                                    INTERIOR OF C-45H WITH FOUR SIMULTANEOUSLY-OPERATED
                                                CAMERAS MOUNTED

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32
Ground support for WERL aerial monitoring and
surveillance responsibilities was concurrently  up-
graded  and  expanded  during 1971.  Newly  ac-
quired properties include a complete line of major
devices for developing, printing and reading film
and a battery of cameras for use in photographic
remote sensing.

Available manpower reserves include  all classifi-
cations of  professional and  craft personnel  re-
quired  for  complete support of any prospective
project assigned  to  the air fleet. Extensive main-
tenance and repair  capabilities are available in-
cluding capacity to  modify airframe structures,
overhaul  engines and  maintain  radio and elec-
tronics instrumentation and switchgear.
                                           GRUMMAN MOHAWK
                                                                                       TH-55 HELICOPTER

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                                                                        33
INTERIOR OFC-123 WITH VEHICLE ABOARD

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34
DOSIMETRY

A network of dosimetry stations is maintained to
monitor gamma radiation around the NTS. Three
EG&G  Model  TL-12  thermoluminescent  dosi-
meters are placed at each of 96 locations and are
replaced  monthly. Sixty off-site residents wear
TLD's,  also  replaced  monthly, to  provide  a
sample of  personnel exposure  levels at off-site
locations.

The TL-12 utilizes a CaF2:Mn phosphor bonded
to a helical heater wire which is encapsulated in a
glass  tube. The tube is enclosed in an energy-
compensating shield of lead-tin-aluminum  which
provides a flat  energy response to gamma radia-
tion  between 70 keV and  1250 keV. Minimum
sensitivity of the dosimeter is dependent on the
magnitude  of  the  competing  background ex-
posure, and can be as low as one mR.

During 1971, the WERL took over the processing
of samples  from the  Radon Progeny  Sampler
which was developed at Colorado State University
for monitoring air in uranium mines. The sampler
is now being used by the  State of Colorado to
monitor concentrations of  radon in dwellings in
Grand Junction.  The sampler,  usually operated
for  one-week  periods,  draws  air  through  a
millipore filter. The alpha  activity collected on
the  filter exposes a  TLD which is read at WERL
on a  Harshaw  Model 2000 reader to assess the
radon  concentration in terms of working  levels.
WERL supplies  the sampling heads  containing
filters  and  TLD's, reads the dosimeters, reloads
the filter heads, and processes the data for about
50 samples a week. The TLD is a Teflon CaF2: Mn
1/2-inch disk with  a  1/8-inch hole through the
center to  pass  air while the sampler is  running.
Sensitivity of the system allows measurement of
background  levels of radon, about 10-4  working
levels.
AIR SAMPLING

Continual surveillance for radioactive particulates
and  gases is maintained through the Air Surveill-
ance Network (ASN) which, during 1971, con-
sisted of 102 permanent stations and 33 stations
activated for specific intervals. Special  networks
are  also  established  for  events conducted at
locations remote from  the NTS: to monitor the
Cannikin  Event, 15 stations were established in
Alaska during 1971. In the event of a release of
radioactivity  from the NTS,  the ASN data are
supplemented by gamma rate  recorders and port-
able air samplers operated by monitors.

ASN air samplers  draw ten cubic feet per minute
through glass fiber filters which are changed every
24 hours. With a sensitivity of 0.06 pCi/m3 for an
average sample, increased  activity  levels  can be
identified many orders  of magnitude less than the
established guides for gross radionuclide con-
centrations in air. In addition  to  the  filter the
samplers are  equipped  to  use charcoal cartridges
for the collection  of reactive gases. During 1971,
approximately 36,000  filters  and 8,000 charcoal
cartridges were submitted for analysis for beta
radiation  and measurement of  gamma-emitting
radionuclides.

In continuing efforts to upgrade the quality and
efficiency of the  ASN, sampling equipment was
rotated on a routine  basis for  maintenance to
allow maximum use with  a minimum of  down-
time. Routine field calibrations were performed
to  assure  validity  of flow  information  and
minimize errors in measurements.
              THE AIR SURVEILLANCE NETWORK SAMPLER •

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35

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36
MILK SAMPLING

Milk is particularly  important as a  medium for
indicating the presence of certain airborne radio-
nuclides.  Being  one  of the  most  universally
consumed  foodstuffs, it is one of  the  most
convenient dietary constituents to collect, handle
and  analyze. During 1971,  three different  milk
surveillance programs were maintained:
   Pasteurized Milk Network

   Sponsored by the EPA in conjunction with
   Federal and  state  agencies,  this network
   provides data on current radionuclide con-
   centrations  and  long-term  trends.  Com-
   posite   samples  of  pasteurized  milk  are
   collected  in proportion to consumption in
   63 specific metropolitan area  milk supplies.
   Those  collected  from 23 such areas in the
   western U.S. are analyzed monthly at the
   WERL.

   Routine Milk Sampling Network

   Supported by the AEC, this monthly milk
   sampling  program  consists  of  about 40
   stations in Nevada,  Utah, and California.
   Samples are  taken  from Grade A dairies,
   family  milk   cows   and   representative
   pasteurized milk supplies.

   Standby Milk Surveillance Network

   Also supported  by  the AEC, this network
   can  provide  additional  information  to
   supplement data from other networks. Milk
   samples are collected  on an  unscheduled
   and timely basis from approximately 200
   processing plants located in states west of
   the Mississippi River.
To  facilitate  surveillance activities, a comprehen-
sive  census  of population  and  milk cows is
maintained. Updated through a periodic survey,
this information is computerized and a directory
is published containing the number of adults and
children, the number of cows, the type of feed
consumed by the cows, whether milk is marketed
and/or consumed by the family, and the precise
location of the collection source by both longi-
tude and  latitude and its azimuth  and distance
from the control  point at the NTS. These surveys
cover all  of  Nevada  and portions of  Arizona,
California, and Utah.
FOOD SAMPLING

The   Institutional   Total  Diet  Network  food
sampling program is designed to provide estimates
of the dietary intake of radionuclides in a selected
population group ranging from children to young
adults of school age. Total diet samples, including
milk,  representative of  the food  intake for one
week  by a specific sex and age group are taken on
a  quarterly basis from  23 participating  institu-
tions.  Samples  are collected  at  ten  of  these
institutions and sent to the WERL for analysis.

The  samples are analyzed for information  on
radionuclide intake  by specific population groups
for studies of exposure, trends, and public health
significance. Analyses include the measurement of
both naturally occurring and artifically produced
radionuclides.

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                                                                                               37
WATER SAMPLING

Water samples  are  routinely collected to deter-
mine  radiological  quality of  both potable and
non-potable  water in  California,  Nevada  and
Utah. In cooperation  with the Bureau of Water
Hygiene, samples are also collected from a variety
of interstate  carrier locations such as depots and
airports and sent to the WERL for analysis.

Gross alpha and beta counts and gamma spectrum
analysis  indicate that,  during  1971,  no water
sample collected contained fresh fission products.
Of  the approximately  25  samples  per  month
routinely  analyzed for  tritium content,  tritium
values  above the Minimum Detectable Activity
(MDA)  were  found  in  samples of  Las Vegas
municipal water. This  water was taken from Lake
Mead  which, like  many surface  water supplies,
characteristically contains tritium concentrations
greater than the MDA  of 400 pCi/l.
The Tritium Surveillance System was initiated in
July 1970 to  measure tritium  content  in water
downstream from nuclear facilities as well as in
drinking water. This nationwide network consists
of  73  drinking  water  and 37  surface  water
stations sampled quarterly. Routinely, 12 surface
and 29 drinking water  samples  were  analyzed
quarterly at WERL. This network was established
in response to  the increased interest in tritium in
the environment as a result of the growing nuclear
power  industry and  its associated nuclear wastes.

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38
SUPPLEMENTAL SAMPLING

Samples of snow, soil and vegetation, particularly
grasses on which cows may feed, are collected in
significant  numbers  within a  few weeks before
and  after nuclear events and occasionally on a
routine basis to  document findings from other
sampling media. Natural gas  samples and special
samples  were taken  as  a follow-up  of  Project
Rulison and  a large  number  of  samples  of milk,
water, soil, vegetation, crab,  salmon and shrimp
were collected during the Cannikin  Event period.
PROJECT RULISON

The WERL conducted the off-site safety program
for Project Rulison, near Grand Valley, Colorado,
which  was  the  second Plowshare  nuclear gas
stimulation  project.  Phase  III  of the  Rulison
experimental  program  involved  the  controlled
drillback into the underground cavity created by
the nuclear explosion in a  natural gas formation,
and flow testing of the gas well to determine the
cavity  volume and the rate at which natural gas
flowed from the low permeability reservoir. The
detonation occurred in September 1969 and the
drillback was completed in October 1970. Flaring
tests, or burning  of  gas from the experimental
well,  began  in  August  1970.  The  natural gas
released during flow testing contained low  levels
of tritium and krypton-85.

After completion of the calibration, high-rate and
intermediate-rate flaring  operations, the  long-
term, low-rate  production  flaring was begun on
February  2  and continued  through April 23,
1971. At least one WERL field representative was
on  duty  in  the Rulison  area throughout this
flaring operation to collect atmospheric moisture
and other environmental samples for radioactivity
analyses. Samples of water, milk, natural vegeta-
tion,  animal  tissue, soil, precipitation and  urine
were  collected  routinely throughout the flaring
operation and again after  the flaring was  com-
pleted. Samples of garden vegetables and orchard
crops were obtained during  the growing season
that followed the flaring operation.  All samples
were analyzed at the WERL.

Aerial  surveillance was  provided  for the special
intensive  surveillance  in  March.  Atmospheric
moisture  and  gas  samples  were collected  by
aircraft in coordination with special sampling for
atmospheric  moisture  on  the  ground  to relate
airborne radioactivity levels to those found on the
ground.
              OBTAINING A GAS SAMPLE AT THE RULISON WELL
                                                                                              -•*•-

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                                                                                             39
Radioactive  effluent from this  event  was not
found in human food products. Based on surveill-
ance results, the maximum postulated dose at a
populated location three miles from the site, was
about 0.001 mrem.
CANNIKIN EVENT

The WERL conducted a radiological surveillance
and safety  program in support of the Cannikin
Event, a nuclear test of less than five-megatons
held on Amchitka Island, Alaska, on November 6.
About two weeks before the event, monitoring
personnel  were  stationed  at  22  communities
representing all inhabited locations from Chignik
on the Alaska Peninsula to Attu, including the
Pribilof Islands,  Old  Harbor and Kodiak Island.
These  monitors  were responsible for  providing
residents with information regarding the test and
for coordinating public safety and information
activities locally. Communications were maintain-
ed by two-way  radio to the Control Point on
Amchitka Island.

A great number of environmental samples, includ-
ing air, water, milk, precipitation, vegetation, soil
and stream  sediment, were collected for analysis.
A  dosimetry and  air sampling network of 15
locations was established. Pre-event marine food-
stuff samples, including salmon, crab, and shrimp,
were obtained from commercial fishing areas off
the coasts of Alaska and Washington. Also, 115
marine  samples, including samples taken  near
Amchitka  during the immediate  pre-event and
post-event periods,  were collected and sent to the
WERL for  analysis. Measurements taken with
survey instruments, recorders and dosimeters in-
            ALASKAN SURVEILLANCE NETWORK AND STANDBY LOCATIONS
                                ARCTIC OCEAN
                            IrTpAULr' °'  KINe SALMON
                                 /,• ST. GEORGE
                                  '•«NOS
                                           CHIGNIK
                ^AMCHITKA   COLD BAY

              %.            UNALASKAItt
                STANDBY LOCATIONS
                \VERL SURVEILLANCE STATIONS FOR THE CANNIKIN EVENT OPERATED BY LOCAL INDIVIDUALS

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40
dicated that  no radioactivity was released to the
atmosphere as a result of the test.

Aerial  monitoring was performed  by four moni-
tors, two each aboard Air Force C-130 aircraft.
At  event  time,  the  two  WERL  teams  were
airborne  near  surface  ground  zero,  and  were
prepared  to  sample  and  track  any  release  of
radioactivity.  Three WERL  monitors were also
stationed on  board two naval vessels and a Coast
Guard vessel  in  the  waters near  Amchitka  on
event day. Each monitor collected air samples and
marine water samples before and after the event.
Additional WERL  personnel  were  stationed in
Juneau and  Anchorage and  on Amchitka.  Over
100 WERL  personnel  participated in this pro-
gram.

Because  considerable public apprehension arose
prior  to  this  nuclear  test,  about  30  WERL
personnel remained at their assigned locations for
at least three days after detonation of the device
or until assurance was established that no public
safety problems remained. Based on  the results
from  the extensive  surveillance program,  there
was no  radioactive effluent released to the bio-
sphere.

In a  joint effort  with the Alaska Area  Native
Health Service,  the  WERL analyzed urine and
blood samples collected from approximately 50
Aleut residents of Atka, about 300 miles east of
Amchitka.  Just   prior  to  the event,  all Aleut
residents of Atka received a whole-body count by
the WERL monitor stationed on the island for the
event.
                                                         LOCAL RESIDENTS OPERATED AIR SAMPLERS DURING CANNIKIN

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41

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42
           SPECIAL STUDIES
ATMOSPHERIC MOISTURE TRITIUM

A field study was conducted at the site of Project
Rulison near Grand Junction,  Colorado, to study
by comparison the concentrations of atmospheric
tritium as  determined from  a  passive system
which removes moisture from  the air by freezing
and an active system which pumps air through a
cannister  of  molecular  sieve for  atmospheric
moisture  adsorption. Both techniques had been
used  for surveillance during the gas flaring phase
of  the  Rulison experiment. Tritium was one of
the  radionuclides released during  test flaring of
the natural gas and after flaring was mainly in the
form of water vapor. Also included  in the study
was an evaluation  of  the  relative  accuracy of
absolute humidities  as  determined from various
psychrometric   measurements and  from  the
molecular sieve sampler assuming  100% removal
of  moisture from   the known volume  of  air
sampled. Results of the study  are reported in the
paper, "Comparison of Freeze-out and Adsorp-
tion  Techniques  for the Collection of Atmos-
pheric Tritium as HTO." The  findings show that
at  the 99%  significance level  no  differences
existed between the  two methods of determining
atmospheric concentrations of  tritium in the form
of water vapor, nor between the techniques used
to determine absolute humidity.
HUMBOLDT BAY PILOT STUDY

A pilot study using aircraft in a coordinated aerial
and  ground sampling project was conducted  at
the  Humboldt  Bay Power  Plant near  Eureka,
California. Airborne  effluent from  the power
plant nuclear reactor was measured to determine
height above the ground and rate of dispersion.
Exposure rate  measurements and  sample collec-
tion  were performed aloft and on the ground for
comparison  to  theoretical  predictions  using
Button's  diffusion  equations. The study showed
that future  surveys will  require more intensive
and  more  accurate  meteorological data to  be
collected. Results of this study show good agree-
ment  between theoretical and  measured results
along  the plume  centerline, but  plume dispersion
across the trajectory was much more than predict-
ed. Plume rise was considerably more than pre-
dicted and  is believed to be the result of terrain
effects. Results of the pilot study are reported in
the paper, "Humboldt Bay Nuclear Power Plant
Survey — March through May 1971."

Additional studies of a similar nature are planned
at the Humboldt Bay Plant and at several  other
sites throughout the country. The C-123 aircraft
will be used for future studies since  it will provide
the capability to transport the vehicle required in
the ground  sampling  and  the laboratory equip-
ment  required  for  analysis of  the samples on
location.
GROUND LEVEL SAMPLING COMPLIMENTS IN-FLIGHT MEASUREMENTS
   EXPOSURE RATES ON THE GROUND CAN BE MEASURED AND
          RECORDED FROM A MOVING VEHICLE

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43

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44
URANIUM MILL TAILINGS PROJECT

During  1971,  the Uranium  Mill Tailings Project
underwent major expansion.  The principal project
efforts are continuing in the joint Colorado-EPA
Indoor  Radon Study  with  primary  focus  on
activities at Grand Junction, Colorado. In July,
the project was expanded to include ten western
states.  The ten   states  included  are  Arizona,
Colorado,  Idaho,  New  Mexico, Oregon,  South
Dakota, Texas, Utah, Washington and Wyoming.
The  initial effort  in the nine additional states, in
cooperation with  the state and coordinated with
the  EPA  Regional  Office,  has been  a mobile
gamma  scan of communities adjacent to uranium
mill  tailings piles with  a highly sensitive truck-
mounted radiation detector. This scan  was made
possible  by a contract  with the AEC  and was
arranged  through  the   AEC   Grand  Junction,
Colorado, Operations Office. The unit is operated
by  the  Lucius-Pitkin   Company  of   Grand
Junction.  Scans in the  states of South Dakota,
Wyoming,  New Mexico, Arizona,  Colorado and
Utah were  completed  by December.  The next
phase  is  to  do  follow-up  evaluations  of  the
locations showing above normal radiation levels,
as reported by the scanning unit.
WERL MONITORS CONTINUE TO MEASURE GAMMA RADIATION IN THE
          HOMES IN THE GRAND JUNCTION AREA
OFF-NTS PLUTONIUM STUDY

Begun  in  1970,  this study  was  designed  to
determine plutonium deposition in the immediate
area around the NTS and in selected areas of the
western United States. During 1971 soil samples
were collected from approximately 150 locations
near  the  NTS  and analyzed  for  plutonium. In
order to  evaluate the resuspension of plutonium
from  soil to the  air, filters from  air samplers
operating near the NTS were analyzed with filter
selection  based   on  meteorological  criteria
supplied  by the Air Resources Laboratory of the
National  Oceanic & Atmospheric Administration.
Soil  and air  sample  analysis  indicates  that
plutonium levels above world-wide fallout levels
exist  immediately  off the NTS;  however,  the
levels  are  well  within  permissible limits  and
present no hazard to humans.

In addition  to  the  near-NTS studies,  annual
plutonium-in-air cycles were determined for eight
locations  in  the western United  States remote
from the NTS. The concentration of plutonium at
these locations indicates only world-wide fallout
levels.

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           45

r\_r
k

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training  and
education
 The Training and Education services offered by
 the WERL encompass a broad curriculum of
 direct, short-term training courses in radiological
 health; continual development of employee skills,
 knowledge  and technical competence; various
 types of training assistance to State and local
 government and other federal agencies and inter-
 change of services with the University of Nevada,
 Las Vegas (UNLV). Of special significance to the
 radiation training program at WERL during 1971
 was the realignment of training courses to reflect
 goals and missions of the EPA.

 Considerable effort was  spent organizing the
 segments of the radiation training program which
 were transferred to EPA. This involved not only
 the establishment of  a functioning organization
 incorporating  many  newly-assumed  responsi-
 bilities previously delegated elsewhere, but also
 the concurrent  presentation of training courses
 which had been planned and scheduled for 1971
 under prior program commitments. Accordingly,
 the radiation training program evolved into  a
 cooperative, coordinated effort between WERL
 and  the  Eastern  Environmental  Radiation
        Laboratory (EERL). A schedule of courses for
        calendar year 1972 was prepared with a shift in
        emphasis to courses involving the environmental
        aspects of radiological health and protection.

        Direct short-term training courses are taught by a
        staff of five full-time instructors either in  the
        well-equipped WERL training facilities or, upon
        request, at  field  locations. During  1971,  the
        WERL presented  sixteen courses, totalling 150
        days of training. Approximately one-half of these
        courses were presented at the WERL and  the
        remainder at field locations in Alabama, Texas,
        New Mexico, Colorado, Arkansas and Arizona.
        Offered tuition free, these courses are open to all
        persons involved in radiological health or protec-
        tion.

        The schedule of short-term training courses pre-
        sented during 1971 is a combination of basic and
        advanced courses  in radiological health and pro-
        tection, designed  to provide either  the funda-
        mental skills and  knowledge  necessary to cope
        safely with the multitude of uses of radiation or
        to  provide  advanced  knowledge  in the more
                     COURSES PRESENTED DURING 1971
           COURSE TITLE
LENGTH OF
 COURSE
 IN DAYS
  NUMBER
    OF
PRESENTATIONS
 TOTAL
NUMBER
OF DAYS
 NUMBER
  OF
STUDENTS
    1. Radiation Protection Guides and
        Dose Assessment	10

    2. Occupational Radiation Protection ... 10

    3. Radionuclide Analysis by Gamma
        Spectroscopy	10

    4. Basic Radiological Health	10

    5. Environmental Radiation Aspects of
        Nuclear Reactors	5

    6. Medical X-Ray Protection	10

    7. Management of Radiation Accidents.  . .  5

                      TOTALS	
             3
             3

             1
             6

             1
             1
             1

            16
              30
              30


              10
              60

               5
              10
               5

             150
            35
            41

            11
           129

            37
            17
            30

           300

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47




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48
specialized phases of the field. Because of  long-
term scheduling, the  training  courses  presented
during 1971 reflected both Public Health Service
and  EPA objectives. Future training courses will
concentrate on  the  environmental aspects of
radiation,  whereas other organizations will be
responsible  for   medical  and  occupationally-
oriented radiation uses.

In addition to the regularly scheduled training
courses  offered  at the  WERL,  another  more
specialized type of training is available. This is a
bench  training  situation   developed  between
personnel of a requesting agency and the WERL.
Tailored to meet the individual's need for training
in procedures,  methodologies or analytical  tech-
niques used within the scope of WERL activities,
the  program  is varied  in  time and coordinated
with the various programs of the WERL.

All  employees of  the WERL are continually
encouraged to increase their professional develop-
ment or improve their job skills through attend-
ance at appropriate meetings, seminars and train-
ing courses. Sixty-five attendees from the WERL
participated in 29 different training courses or
seminars; 36 professional  meetings were attended
by 38 participants. Five personnel were enrolled
for at least one semester in courses at the UNLV.
Monitor   certification  training  and   refresher
courses  for WERL employees  who   serve on
short-term assignments  as monitors are also offer-
ed.
      PROFESSIONAL EXPERIENCE
              OF STUDENTS
          YEARS OF
         EXPERIENCE

         Less than 1
NUMBER OF
STUDENTS

    26
             1-5
   112
            6-10
    64
            11-15
    41
            16-20
    31
        Greater than 20
    26
                     TOTAL  300
                          Because  of  their proximity, UNLV and  WERL
                          maintain close relationships, both on a formal and
                          informal basis, in  areas of mutual benefit. This
                          may  be  in  the  form  of  free  exchanges of
                          information, technique advice and consultation
                          services  between the professional  staffs, or by
                          jointly sponsoring  meetings or symposia. Five of
                          the WERL instructors taught courses at UNLV as
                          Adjunct  Professors and,  concurrently, four  pro-
                          fessors from UNLV were part-time staff members
                          at the WERL. In addition to these types of joint
                          efforts,  due to classroom and equipment prob-
                          lems created by the rapid growth of UNLV, 658
                          hours of UNLV  classes  were held   in  WERL
                          facilities  and some WERL equipment was used for
                          special UNLV projects.

                          Also, the President of  UNLV and  his  senior
                          faculty met  with the WERL staff for an orienta-
                          tion briefing  of  WERL operations  and capa-
                          bilities,  a brief  discussion  of  possible areas of
                          mutual cooperation and  a  tour of WERL facil-
                          ities.
                             DEGREES HELD BY ATTENDEES
                                      BY PERCENTAGE

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                                                                          49
     STUDENT DAYS AND
  DAYS OF TRAINING vs TIME
  1965  J966	1967	1968   1969   1970	1971
  COURSES AND
STUDENTS vs TIME
                                                                     \ .
                                             I    I    I    i    I    I    I
                                             1965   1966   1967   1968   1969   1970   1971
                          ATTENDEES BY STATE
ALASKA	0
HAWAII	8
CANADA	1
WASH., D.C	2
CONT'L U.S	289
    TOTAL:  300

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research
During 1971,  the WERL  conducted extensive
field and laboratory experimental studies into the
ecology of specific pollutants and the methods
and effectiveness of the transport to man through
the various media. In these studies, collectively,
the WERL demonstrated research and develop-
ment capabilities  which are readily adaptable to
in-depth studies in other kinds of environmental
pollution. However, research efforts of the WE R L
were principally applied to investigations into the
causes, characteristics  and  effects of manmade
and natural radiation.

Environmental  transport and ecological  studies
being  highly dependent on precise analytical
methods, sophisticated and, in several instances,
specially-designed  analytical apparatus and instru-
mentation are maintained for data collection and
processing. The analytical techniques  used are
continuously evaluated and, as new requirements
arise, new procedures are developed accordingly.
Similarly, biometric support and instrument de-
velopment are regarded as necessary to supple-
ment the research efforts. Facilities support for
WE R L research projects includes a 30-acre experi-
mental farm maintained at the Nevada  Test Site
and a greenhouse for plant and soil  studies which
is a part of the WERL complex.

A section of the 16-1/2 acres under cultivation on
the NTS farm is divided into experimental micro-
plots which  are used to evaluate the uptake of
low quantities of aged mixed fission and  activa-
tion products in locally grown truck vegetables.
                         THE LABORATORY-BARN AT THE EXPERIMENTAL FARM

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51

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52
The balance of the croplands is used primarily for
forage crops for  the  30-head experimental dairy
herd. An experimental beef  herd,  averaging 70
head and managed by farm personnel, roams the
mountains, valleys and dry lakes of the desert test
site.  In  addition  to  these  plant and   animal
husbandry  capabilities,  research   facilities  are
maintained  in the WERL complex for support
studies  with  goats, swine and other animals and
for controlled environment studies with plants in
the greenhouse.

In the past, efforts were concentrated on studies
related  to radioiodine, then gradually extended to
include other radionuclides such as radiotungsten,
tritium,  carbon-14  and   plutonium.  With  the
expanded scope  of the WERL mission under the
EPA, present capabilities of the WERL are being
utilized, particularly  in the  field of radiotracer
techniques,  to solve other environmental  prob-
lems.

Most of the  1971 research effort  was devoted to
evaluating the transfer of radionuclides through
the  fallout-air-forage-cow-milk-man chain.   A
major means  of transfer of radionuclides to man
being milk, dairy cows from the farm are used for
radioisotope ingestion and inhalation studies. In
another area of research,  samples  are regularly
collected from domestic  and  game  animals and
analyzed  for  a variety  of  radionuclides.  The
domestic animals are generally selected from  the
beef herd which grazes relatively freely on  the
NTS. To  evaluate  possible  effects  of  off-site
nuclear  events,  such as Project  Rulison,  back-
ground and post-event animal samples are collect-
ed  in  the area surrounding  the test location.
Findings from these samplings  can be used  to
predict  effects on  the human population in  the
same geographical region.

The results of WERL studies are supplemented by
field and laboratory investigations in soil, plants
and  other  media.  To  complete the ecological
cycle, aerosol  studies are conducted to simulate
the fallout  particulates and study the relationship
between their  size  and shape and  chemical  or
physical form and any potential hazard.

Although  not described in detail, the following
specific examples  are  typical of  the  research
projects conducted at the WERL.

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                                                                                                 53
ANIMAL STUDIES

Biological effects of ionizing radiation are studied
in depth.  Two  current  projects  use  rats  and
hamsters.

To  determine  whether polonium-210, a  radio-
active element found  in tobacco, may be a factor
in the formation  of lung cancer, rats are exposed
daily  to   cigarette   smoke  containing   added
polonium-210.  Most of this element in cigarette
smoke is cleared  from the rat lung soon after a
single exposure;  however,  continued  daily ex-
posures  result in an equilibrium concentration of
polonium in the lung.

Syrian  hamsters  are   used  in  a  study  of the
potential health  hazards associated with the in-

          THE CIGARETTE SMOKING MACHINE EXPOSES RATS TO
                   POLONIUM-210 IN SMOKE
halation  of a  single highly-radioactive  particle,
such as those emitted by reactors developed for
nuclear rocket engines. To obtain biological data
to develop reliable safety standards, seven groups
of hamsters are studied: three groups containing
pulmonary-deposited radioactive  particles,  one
group  containing inert particles and three groups
serving as controls.  In conjunction  with serial
sacrifice to follow the development of the expect-
ed primary lung tumor, gamma spectroscopy and
radioautography are used to follow the  develop-
ment of effects. Gross lesions, apparently primary
lung tumors,  were found in two animals  in the
high dose group. Pathological evaluation  is still  in
process. A lung tumor was diagnosed, histological-
ly, in the middle dose group.

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54
             EXCRETIONS ROUTES FOR A VARIETY OF RADIOISOTOPES
                                (Average for four cows)
ISOTOPE AND
COMPOUND
86RbCI
202TIN03
181W04
129Te203
203Pb(N03)2
59FeCI3
203HgCI3
7BeCI2
ROUTE OF
ADMINISTRATION

Oral
Oral
Oral
Oral
Oral
Oral
Oral
Oral

MILK
15
2.5
0.64
0.3
0.014
0.013
0.012
0.0016
TOTAL % ADMINISTERED
DOSE RECOVERED
URINE
64
7.7
17
4.1
0.12
0.009
0.075
0.023

FECES
21
37
63
76
93
77
75
68
                      COWS ARE KEPT IN INDIVIDUAL STALLS DURING METABOLISM STUDIES

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                                                                                                  55
Bio-kinetics investigations  are also conducted  at
WERL. Studies defining critical  metabolic  path-
ways in animals of ingested radionuclides which
could  appear  in  the  environment  following
nuclear detonations provide valuable data for use
in  hazard  evaluation.  Because  dairy  products
contribute  a significant portion of the total diet,
particularly for infants and children, and  may be
the  major  route of  radionuclide exposure, such
studies are conducted with dairy  cows.  Metab-
olism studies in cows  have the added feature  of
supplying the constants for models to predict the
extent of  man's  probable exposure as  well  as
giving  indications  of specific biological availabil-
ities, potential  sites of tissue concentrations, and
major  routes of  excretion. To  obtain  adequate
data for  hazard assessment, these studies  include
both single and multiple ingestion exposures by
dairy cows  for each nuclide studied.

Tissue distribution studies indicate the principal
sites of major deposition for the  radionuclides  to
be as follows:
   Rb    liver  >muscle  >bone  >lung  >spleen
   Tl     kidney >lung  >muscle  >bone
   W     bone  >pelt  >liver  >muscle
   Hg    kidney >liver  >spleen
   Be    liver  >kidney  >bone
An artificial rumen device is used to  measure the
in vitro solubility of various chemical compounds
in rumen  contents. Compounds  which are  less
soluble in vitro are also secreted to a lesser extent
in milk in vivo. This procedure facilitates estimat-
ing transfer to milk, since one compound  can be
tested  in a metabolism study and other chemical
forms  of the same  element can then  be tested in
the artificial rumen.

Another animal  used  in these  studies  is  the
lactating goat which is small enough for  labora-
tory facilities and  simplifies the study of  milk
secretion of radionuclides prior to such studies in
dairy  cows.  Goats given an  injection  of pluto-
nium-238 excreted  about one percent in the milk,
and analysis of tissues indicated the liver had the
highest concentration and bone the next highest.
When cheese was made from the milk, nearly all
the plutonium in the milk appeared in the cheese.
This effect  was independent  of storage  time or
state of pasteurization of the milk.

    GOAT STUDIES ARE CONDUCTED IN THE WERL FACILITIES
Tritium, a  radioactive isotope of  hydrogen, is
gradually   becoming  more  abundant   in   the
environment.  Since  it  is  incorporated  in all
biological processes, tritium must  be evaluated to
assess  its possible hazard.  The dose equivalent
from tritium is usually calculated  by assuming its
effective half-life is the same as the half-time for
body  water,  but  evidence from occupational-
exposure cases indicates that some portion of the
tritium has a longer  effective half-life. In three
females who worked in a plant where watch dials
were  painted  with  a tritiated   luminous com-
pound, the change  in tritium body burden  was
followed for an extended period of time. Tritium
initially was excreted with a biological half-life of
about  one week which was then  succeeded by a

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56
half-life of about one  month. At the end of the
study, the tritium  was  being excreted with  a
biological half-life of  280 days to 550 days. A
comparison of the biological half-life of tritium in
lactating  dairy  animals  and  non-lactating  beef
animals was an objective of another study. Addi-
tional information on the relative biological half-
lives of tritium in  the different components of
milk from dairy cows resulted from these experi-
ments. The tritium in beef animal blood exhibited
two half-lives, i.e., 2.8 days initially followed by a
longer component of 29 days. This longer com-
ponent  in the dairy animal ranges from  about 25
days in  milk  protein to about 30 days in the milk
serum and about 60 days in the milk fat.

Two studies are in progress to determine whether
the results mentioned above are due to tritium
incorporation in organic components. One study
uses rabbits and the other uses two species of fish.
Both  are  based on maintaining  an  experimental
population in a uniformly tritiated environment
for two to three generations  and determining the
specific  activity  of  hydrogen  in the organic
components of each generation.
    TRITIUM CONCENTRATIONS
     AMONG MILK FRACTIONS
o
10


10°


10'


102


io3
                                 PROTEIN
                                 	FAT
                                 •-SERUM
TB2
       20  40   60   80  100  120  140  160
           DAYS AFTER ADMINISTRATION
            STUDIES ARE CONDUCTED ON FISH KEPT IN A
                   TRITIATED ENVIRONMENT

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                                                                                                 57
ECOLOGICAL STUDIES

Ecological studies are conducted on a continuing
basis with concentrated efforts on various trophic
levels in the  environment. In one  such study,
rumen fistulated cattle are allowed to graze freely
in selected  locations for  short  periods of time.
The  rumen  contents are  then removed through
the fistula and  examined for botanical  and radio-
nuclide  content.  The locations used  are those
contaminated accidently, as from  the  Baneberry
Event,  or   where  long-lived  activity, such  as
Plutonium, is known to be present.

Many studies are  underway to investigate  tritium
movement in soil, soil to plant transfer of tritium,
and distribution of tritium between  the aqueous
and organic  fractions of plants.

Results  of  such  laboratory studies  are  directly
applicable to  field  studies such  as those  con-
ducted   around   nuclear   power  installations
(Humboldt  Bay)  and nuclear stimulation of gas
fields  (Rulison).  In  these  studies,  extensive
samplings are made of soils, plants and  animals to
determine transfer dynamics and any  concentrat
ing mechanisms so that critical pathways affecting
radiation exposure may be detected.

The  movement of tritium through  desert soil at
the NTS was also studied. Previous experience has
shown  that various  materials leach  differently
through disturbed and undisturbed  (in situ) soils.
Plots of bare soil and soil  with natural vegetation
were  spiked with tritium  and irrigated regularly
thereafter.  Samples  of vegetation  and of tran-
spired water were collected to evaluate soil losses
by irrigation.

These studies can be extended most conveniently
in the laboratory. In this case, various types of
soils are placed in appropriate containers to study
movement  and absorption  of nuclides as a func-
tion  of soil  type and amount of water applied.
                                                              ONE OF THE FISTULATED STEERS FROM THE BEEF HERD

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58
Soil  microflora may play an important role either
in the movement of materials through the soil or
in transfer of materials from soil to  plant. The
latter role  may  be critical in  the  transfer  of
radionuclides. Studies being conducted  identify
the  microbiota  by  species and numbers  as  a
function of  soil  and plant type and the  solu-
bilizing  effect or uptake of radionuclides by single
species cultures isolated from NTS soils.

Domestic and wildlife tissue samples were collect-
ed for tritium and carbon-14 analyses prior to and
following flaring operations at  the Rulison site.
Collections are continuing in an effort to docu-
ment the  effect  of  these  operations  on this
portion of the human food chain.

The strontium-90 content  of bones from Bighorn
sheep and beef cattle continued the decline which
started in 1964.
                                           APPLIED RADIATION SAFETY

                                           Tritium-activated  luminous compounds are being
                                           used extensively in consumer products and some
                                           have specific activities as high  as 1.2 Ci/g. The
                                           possible hazard from tritium release by many of
                                           these compounds is being evaluated in the labora-
                                           tory. As much  as 0.01% is released daily by the
                                           compounds with  some  indication that this per-
                                           centage decreases with time. Field studies during
                                           the  Rulison  testing  period  suggested that per-
                                           sonnel  wearing watches with tritiated luminous
                                           dials excreted more tritium than other personnel.
                                           A laboratory study with volunteer subjects was
                                           initiated to quantitate the tritium uptake from
                                           wearing such watches.
                                                     SOIL SAMPLES ARE STUDIED IN THE GREENHOUSE
       COMPARISON OF 90Sr IN BONES OF DEER, CATTLE AND BIGHORN SHEEP
        20
      O)
      CO
      o
      o
      a
      I
      CO

      LLJ
      z
      o
      CD
10
                                                                                 DEER
                                                                                 BEEF
                                                                      BIGHORN SHEEP
               1964
                 1965
1966
1967
1968
1969
                                                                          1970
                                                                            1971

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                                           59
METHODS DEVELOPMENT

Procedures are updated  or  generated anew on a
continuing basis in terms of  current requirements:
  • A  solution using two detergents, Triton
    N-101  and Triton  H-55 can  be used to
    make untreated  urine samples soluble in
    the liquid scintillation cocktail.  This  pro-
    cedure permits rapid urinalysis for tritium
    with minimum losses from sample prepara-
    tion.

  • A standard procedure for low-level carbon-
    14 analysis is the combustion of the sample
    to  produce C02 followed by conversion of
    the CO2 to benzene. The CO2  is reacted
    with a metal to form a carbide which  is
    treated with water  to form acetylene. A
    vanadium  catalyst  is then used  to poly-
    merize  the  acetylene to  benzene.  The
    procedure used at  WERL is distinguished
    by  the conversion   of over  90% of  the
    original C02 to benzene and the ability to
    obtain 15  to  20 ml of benzene from a
    single reaction.

  • A  rapid method  for plutonium analysis in
    biological samples has also been developed.
    The  sample is  wet-digested and the pluto-
    nium  coprecipitated  with   appropriate
    agents. After filtering, the  precipitate  is
    solubilized  and the plutonium  extracted
    with triisooctylamine which is incorporated
    into scintillation liquid.

  • Carbon-14 and tritium analyses can both be
    performed  on  a sample by procedures
    recently  developed.  The  sample is com-
    busted in a Parr bomb  to form carbon
    dioxide and water and both  fractions are
    then collected for analysis.

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 informational
activities
 The value and usefulness of WERL surveillance,
 research efforts and investigations are dependent
 on the timely release of information on the
 methods and findings. Increasing public interest
 in the quality of the environment also demands
 that  concise information on WERL programs,
 activities and plans be promptly available. These
 services are provided  through the technical re-
 ports  office, which  includes technical editing,
 typing and illustrations capabilities, the technical
 reference  library,  and the public affairs office,
 which includes professional  photographic  and
 press  and  public  oriented  communications
 capabilities.

 During 1971, twenty WERL  technical reports
 were published, all of which are available to the
 public through the  National  Technical Infor-
 mation Service (NTIS). Other publications  pre-
 pared  and distributed include the WERL  1970
 Annual Report, 38  technical papers for  oral
 presentation or publication in journals, magazines
 or symposium proceedings; updating revisions of
 the  Milk Directory; numerous  abstracts on
 WE R L-generated  papers;  and several  inhouse
 procedures manuals. A brochure outlining WERL
 aerial  surveillance capabilities  was  initiated.
 Surveillance  data collected  routinely  by  the
 WERL were  condensed from six-month reports
 for publication in Radiation Data and Reports.

 In addition to numerous subscription periodicals,
 trade journals and EPA  publications, including
 reports of air and water pollution studies, the
 WERL  library keeps  copies of all  WERL-
 generated  reports, a wide variety of textbooks
 and other hardback  reference  material.  The
 borrowing arrangement with the libraries in the
 southern Nevada area, the University of Nevada,
 Las Vegas,  the  Southern  Nevada  Memorial
 Hospital, the AEC and  AEC  contractors, con-
 tinued through 1971  and included interlibrary
 loans arranged with UCLA and the AEC library at
 Oak Ridge. During 1971 also, the library became
 part of the  interlibrary  data  exchange system
 involving all  40 of  the EPA libraries, and a
 microfiche and microfilm reader-printer was pur-
chased for the WERL library to facilitate data
research and utilization.
     LIBRARY TRANSACTIONS 1971
  LIBRARY MATERIAL

      Books

      Reports

      Reprints

      Patents
REQUESTED

  259
  588
  1469
   19
RECEIVED

  215
 1829
 1383
   54
In accordance with EPA policy, a function of the
WERL Office of Public Affairs is to provide
information about the Agency and its activities to
anyone who requests it. As the WERL became
more strongly identified with the EPA during
1971, increased public awareness stimulated an
increasing number of inquiries on air and water
pollution, solid wastes, pesticides and noise abate-
ment problems in addition to those of radio-
logical health nature. These inquiries came from
individual citizens, public  officials,  communi-
cations media, citizens groups, legislators, educa-
tional institutions, libraries, students and business
concerns. Response  was made through direct
correspondence, telephone  and personal inter-
view, published reports, informational brochures,
films, news releases, briefings and public speaking
engagements, exhibits,  displays  and  tours of
WERL facilities, and by reference to the appropri-
ate EPA program or Regional Office. The WERL
also  provides  information,  particularly  to
scientific and technical audiences, by sponsoring
and/or participating  in scientific meetings and
symposia, and through  news coverage of these
events.

On August 30 through September 2, the WERL
co-sponsored with the University of Nevada, Las
Vegas, a Tritium Symposium which was attended
by  about 400 persons  from throughout  the
United States and from ten foreign  nations.
Nearly 100 papers were presented on sources of

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                                                                                                                        61

MANY GROUPS TOURED THE WERL FACILITIES DURING 1971 RANGING FROM SCHOOL CHILDREN TO DISTINGUISHED VISITORS FROM WASHINGTON
                                                                                           /             n

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 62
                              NOBEL-PRIZEWINNER DR WILLARD E LIBBY WAS A FEATURED
                                     SPEAKER AT THE TRITIUM SYMPOSIUM
tritium and predictions of future production and
possible environmental  levels,  behavior of tritium
in the environment, biological  effects, methods of
detection  and  analysis,  dose  assessment,  and
problems  of  disposal.  Proceedings  of  the  sym-
posium will be published in 1972.

A workshop  in  Environmental Quality Sensors
was  held  at the WERL, November 30 through
December  3,  and attended by 55  persons  from
within EPA and from other Federal  agencies and
EPA contractors and grantees. Sponsored by the
EPA Office of Monitoring, the meeting provided
attendees an opportunity to learn firsthand about
the  monitoring activities  and requirements of
EPA and. to exchange ideas about monitoring and
remote sensing techniques.

Speakers on EPA and  WERL  activities and  pro-
grams were provided to 16 public, civic or student
groups during  1971,  including local chapters of
the  National  Health   Federation,  Kiwanis  and
Lions Clubs, the Library Association, the Nevada
Public Health  Association, university and college
classes, the Clark County Environmental Health
Task  Force, and others. Also, on 13 occasions,
WERL personnel were interviewed on broadcast
media.

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                                                                                               63
Twenty-four news releases were issued and three
news conferences were held. Nine interviews were
arranged with press representatives which resulted
in articles on WERL research activities, its partici-
pation  in the Cannikin  nuclear test, the Medical
Liaison Officer Network, and other projects.

Through the Information Office, exhibits were
provided  for  National  Library  Week for  the
Federal   Career  Information Day,  and for  the
annual   conference of  the  U.S.  Civil Defense
Council. Bjg Sam, a fistulated steer from the beef
herd maintained by the WERL on the NTS, was
featured in an  exhibit  at  the  AEC-sponsored
Youth  Science  Day  in  Las  Vegas,  at  the White
Pine County Fair in Ely, Nevada,  at the New
Mexico  State Fair  in Albuquerque, and at the  Las
Vegas Jaycees State  Fair. The  recorded story of
Bjg Sam was carried  in spot announcements on
radio  and  television  on each of  these occasions
and some 20,000 fliers and  brochures describing
the beef herd and the experimental dairy farm
were distributed.

During   1971,   16,500  additional  information
documents were mailed in response to inquiries,
picked up by visitors to the WERL or handed  out
to groups touring the WERL. Tours of WERL
facilities, employing about 275 manhours of staff
time,  were  conducted  for  925  persons  in  40
groups and for a number of others in small groups
or as  individuals. Twenty-one  of the tour groups
visited the NTS farm.

Over  100 formal requests for  photographic serv-
ices were received  and 434 new  negatives and
35mm slides were added to the WE R L files. More
than  1500 photographs were  distributed,  many
used  to  illustrate textbooks, reports, and  news-
paper  and magazine articles and as visual aids for
lectures,  speeches,  and technical  presentations.
Photographs were provided to  EPA  Region  V for
an  environmental  exhibit   for   the   National
Association  of  Conservation   Districts, a  slide
series  on the Indoor  Radon Project  in Colorado
was provided for EPA Region VIM, and  color
transparencies with a taped narration by Bjg Sam
were  provided for a  permanent exhibit on animals
in, research  to  be  displayed   at the  American
Museum   of  Atomic  Energy in  Oak  Ridge,
Tennessee.
                                                                     BIG SAM AT THE NEW MEXICO STATE FAIR

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64
As the WERL's mission continues to broaden into
new areas of environmental research and monitor-
ing,  its capability  for providing information and
responding to public inquiry concerning environ-
mental  matters is  also broadening. The  WERL
expects in  1972 to become an even more respon-
sive   resource  for environmental  information,
particularly for information on  the research and
monitoring  which  is  essential  to  carry out the
Agency's  mission of promulgating and maintain-
ing environmental quality for us all.

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                                       This  report makes no warranty or representation, expressed or  implied, with respect to the accuracy.
                                       completeness, or usefulness  of  the information contained  in this report, or that  the use of any infor-
                                       mation, apparatus, method, or  process  disclosed rn this report may not  infringe privately owned rights;
                                       or  assumes any liabilities with respect to the use of, or  for damages resulting from the use of any
                                       information, apparatus, method,  or process disclosed  in this report.
                                                               TO NELLIS. VALLEY OF FIRE SALT LAKE CITY
                                                                 AND ZION-BRYCE
                                                                 NATIONAL PARKS
TO Ml. CHAILf STON.
DEATH VAUlY, IENO
AND SAN FIANCISCO
                                  UNION PLAZA 0/0-CASINO CENTE
                                         SAHARA
                                         ii   KAREN AVI
                                         I—I
          CAESARS PALACE QO FLAMINGO
 1O LOS ANGEIES (790 MILES) I
                                                                                                       OFFICE OF TECHNICAL  REPORTS. AERL

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