Biological Study of the Fox and Wisconsin Rivers
                        by

                Douglas W. Grothe
              Charles S. Steiner Jr.
                 U.S. EPA Region V
            Central Regional Laboratory
                    October 1978

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                              INTRODUCTION




     At the request of the Water Division and in cooperation with the




Western District 'Office, the Central Regional Laboratory Biology Section




was asked to conduct a biological survey of the Fox and Wisconsin Rivers




at Portage, Wisconsin.  This was done in support of an Environmental Impact




Statement (EIS) currently in preparation.




     This report includes biological findings during three different sampling




periods, namely June 12-16, July 10-13, and August 14-17, 1978.   In addition,




to general physical chemistry such as temperature, pH, dissolved oxygen and




specific conductance, the biological parameters collected include phytoplankton,




zooplankton, periphyton, macroinvertebrates, and chlorophyll.  During the




August sampling period, fish sampling was conducted on both rivers.




     It should be noted that on the last day of the June survey, the field




notebook describing the sampling locations and results of general chemical




analysis performed in the field was lost.  Biologists in the field reconstructed,




to the best of their knowledge, all information that was included in the




notebook.  Because most of the data had been collected within a two day period,




the biologists are confident in their reconstruction descriptions of each




sampling location as well as the general chemical and physical data collected.

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                                 SUMMARY




     Biological conditions in the Wisconsin and Fox Rivers varied from




station to station and from one sampling period to another, resulting in




a shift from moderately enriched conditions (Mesotrophic)  to more seriously




enriched conditions (eutrophic).  The following is a brief classification




of each of the three stations studied on both rivers.




     1.  Station one on the Fox River  was considered to be moderately to




         heavily enriched, while the Wisconsin River at Station one was




         classified as being moderately enriched.




     2.  Both rivers at Station two appeared to be somewhat similar in terms




         of biological productivity during the June and July sampling period.




         However, in August, there seemed to be a  trend on the Fox River




         toward a greater number of pollution tolerant forms, while the




         Wisconsin River remained relatively constant.




     3.  The Wisconsin River at Station three was  more enriched as compared




         to the same station on the FOX River throughout most of. the study




         period.  During the latter part of the study both rivers showed a




         decline in water quality as shown by the  disappearance of certain




         pollution intolerant forms.

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                          STATION DESCRIPTION




Fox River




     Station one was located approximately 3/4 of a mile downstream of




Swan Lake.  The River, at this point, was 20 meters wide with a depth of  one




meter.  Submerged aquatic vegetation covered the sampling area.  The bottom




substrate consisted of soft and "mucky" organic matter with numerous empty




snail shells.  The surrounding land was a marsh-type area with numerous




grasses, cattails, and shrubs lining the shoreline.




     Station two was located at Highway 33 East Bridge approximately one-




quarter of a mile downstream of the Portage Sewage Treatment Plant.  The




channel width was 15 meters, with a depth of one meter.  The bottom substrate




consisted of sand -    and "mucky" organic matter.  The shoreline was lined




with tall grasses and small shrubs.  Submerged aquatic vegetation covered




the sampling site.  At certain times of the year, duckweed lines the sides




of the channel.




     Station three was located downstream of the Portage Sewage Treatment




Plant at the Clark Street Bridge.  The channel width was 15 meters.  The




depth of the river was one meter with a bottom substrate consisting of  rock,




sand, "mucky" organic matter, and numerous empty snail shells.  The shoreline




was lined with grasses and cattails.  At times, duckweed lines both sides




of the channel and submerged aquatic vegetation covers the sampling site.








Wisconsin River




     Station one was located approximately one mile upstream of Highway 78




Bridge at the point where the river starts to bend on the left bank.  An




island was located one-half mile upstream of the station where the river




seperates into two channels.  Channel width was 250 meters.  The bottom




substrate consisted of fine to coarse sand.  Numerous trees and shrubs lined

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the left bank of the river, while a sandy shoreline with grasses  lined




the right bank.  Due to the width of the channel,  three substations  were




located along a transect across the river.  Substations A and C were




located 50 meters from the right and left bank respectively,  while substation




B was located in the middle of the channel.




     Station two was located across from the radio station WPDR antenna




on the right bank of the river.  The channel width was 200 meters.  The




bottom substrate consisted of fine to coarse sand.   Channel depth  was between




1.0-1.5 meters.  This section of the Wisconsin River consisted of numerous




sandbars and islands interdispersed with very shallow areas.   The main  flow




of the river appeared to be along the left bank. The shoreline of both  sides




was lined with numerous trees and shrubs.  Due to  the width of the channel,




three substations were taken along a transect across the channel. Substations




A and C were located forty meters from the right and left banks,  respectively.




Substation B was located .in the middle of the channel.




     Station three was located adjacent to the public boat access landing




at Dekorra Park.  The channel width was 200 meters with a bottom  substrate




consisting of fine to coarse sand.  Three substations were located along  a




transect across the river.  Substations A and C were located forty meters




from the right and left banks, respectively.  Substation B was located  in




the middle of the channel.  The depth at this station was 1.5-2.0 meters.




The shoreline along the left bank was lined with numerous trees,  whereas  the




right bank was lined with sandstone  cliffs.

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     00
                       (WPDR
                        Radio tower
               DeKorra Park
               Boat Landing
                                                        A
Figure 1.  Map of Fox and Wisconsin Rivers showing
           biological sampling sites.

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                                 METHODS
Macroinvertebrates
     Macroinvertebrates referred to in this report are  aquatic  organisms
that can be retained by a U.S. Standard No. 30 sieve (28 mesh per inch) and
live at least part of their life cycle within or upon underwater substrates.
     Qualitative raacroinvertebrate samples were obtained by washing organisms
from rocks, logs and other substrates at the sampling sites. Organisms were
washed into a Surber sampling net.  All organisms collected were placed into
a quart glass jar and preserved with 5% formalin.
     Quantitative macroinvertebrate samples were obtained by using a Ponar
dredge.  The samples were washed in the field through a No. 30  mesh sieve.
All organisms collected were placed into a quart glass  jar and  preserved
with 5% formalin.  Two indepdendent samples were taken  at each  sampling station.

Phytoplankton
     Phytoplankton refers to microscopic plants (algae) suspended in a
body of water that are incapable of sustained mobility  in directions counter
to the water currents.
     Phytoplankton samples were obtained by taking a grab sample at the waters
surface using a 500 ml plastic bottle.  Samples were preserved  with lugols
solution.

Zooplankton .
     Zooplankton refers to the microscopic animals of the plankton community
which graze upon the phytoplankton as a source of food.
     Quantitative zooplankton samples were obtained by  using an eight liter
Nlskin bottle.  Four independent grab samples were obtained approximately
one meter below the waters surface at each station.  The contents of each
                                     6

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Niskin bottle were then pooled.   The organisms  in  the pooled samples were




concentrated by passing the sample through a  plankton funnel fitted with




a 53 p mesh net.  The sample was placed into  a  500 ml plastic bottle,




appropriately labeled and preserved with 5% formalin.









Chlorophyll




     Chlorophyll refers to all plantlife containing  a pigment known as




chlorophyll^.  The measurement of this pigment  can yield  some insight into




the relative amount of alga standing crop. Chlorophyll samples were prepared




in the field by filtering a known aliquot of  water through a Gelman A/E




glass fiber filter.  A MgCO- suspension was then filtered through to prevent




the sample from becoming too acidic.  The filter was immediately  wrapped  in




aluminum foil and placed inside a metal cannister  which had been  appropriately




labeled.  The sample was then placed on dry ice for  transport back to the




laboratory.









Periphyton




     Periphyton refersito an assemblage of organisms that grow  on underwater




subsrtates, and includes such organisms as algae,  molds and protozoa.   All




of the organisms are not necessarily attached to the substrate  but at  least




live in association with attached organisms.




     Periphyton were collected on I"x3" glass microscope  slides by means  of




a periphytometer.  The length of exposure was four weeks, at which time the




slides were retrieved.  The slides collected  were  placed  into a 100 ml




plastic bottle and preserved with lugols solution.

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Fish-Electrofishing




     A boat mounted pulsating direct current electrofishing  unit was




utilized.  (Colfelt electronics model W-20).   The  electric  current was




directed into the water through a pair of steel cables which were  suspended




from booms extending from the bow of the boat.   Each sampling  area was




electrofished for a period of between 20-26 min.




     All stunned fish were dip-netted from the  water and  transferred  to  a




holding tank, where they recovered from the electroshocking.  All  fish captured




were identified to species when possible, counted,  measured, weighed, and




returned to the water.









Chemistry




     Dissolved oxygen and temperature were measured using a  YSI model 54A




oxygen and temperature meter (Yellow Spring Instrument Co.).  The  oxygen




meter was calibrated daily against the lodometric method  for D.O.  analysis




as outlined in Standard Methods for the Examination of Water and Wastewater,




14th edition 1975.  The pH was measured using an Oion lonalyzer model 407A




pH meter.  Specific conductance was measured using  an Industrial Instruments




model RB3 Solu Bridge conductivity meter and probe.
                                    8

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                            CUSTODY PROCEDURE




     Custody procedures were followed throughout the study.  ^.AIl samples

                                                          X"
                                                         /

collected at a particular station were immediately placed into ice chests.




While the stations were being sampled, ice chests were kept  secured in a




government vehicle.  At the end of each day,  all samples  collected were




recorded onto custody sheets and signed.  Samples were returend to the ice

                                                    ./'


chest and sealed with custody labels for transport back to the laboratory.




Back in the laboratory, custody procedures were followed  as  prescribed by the




Central Regional Laboratory Custody Manual.








                                RESULTS




Chemistry




     General chemistry data is presented in Table I.  As  can be seen,




specific conductance concentrations were greater in the fox River than the




Wisconsin River.  Between June and August, specific conductance concentrations




in the fox River ranged between 360-560 yohms/cm compared to a range of




140-160 yohms/cm in the Wisconsin River.  The pH and temperature were similar
                                                               •



in the two rivers.  The two river systems did differ with regard to the amount




of dissolved oxygen present at the various stations.  In the Wisconsin River




dissolved oxygen concentrations remained between 7.1-8.8 mg/1.  However,  in




the Fox River, a significant oxygen sag was present at Stations two and three,




located downstream of the Portage Sewage Treatment Plant.




     In July and August, Station two had dissolved oxygen concentrations of




2.6 to 3.8 mg/1, while Station three had concentrations of 2.2-3.9 mg/1.




In June, Station two had a D.O, concentration of 7.0 mg/1.  This higher D.O.




concentration compared to the values obtained in July and August could be




due to the higher D.O. water from Station one (12.2 to 14.A) flowing into




Station two.

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Fish




     Results of the fish shocking are presented in Tables 2, 3, and A.




Five species of fish were captured from station one on the Fox River.   The




most abundant species was Lepomis microlophus (Redear sunfish).  A total




of four fish were caught.  The other species present were bowfin (Amia  calva),




green sunfish (Lepomis cyanellus), brown bullhead (Ictalurus nebulosus) , and




yellow perch (Perca flavescens).   Fifteen young of the year sunfish were




also retrieved but these were not identified to species.




     In the Wisconsin River, only two species of fish were present at




Station one.  The most abundant fish present were minnows, which were not




identified to species.  One large (64 oz.) quilback carpsucker (Carpoides




c.yprinus) was caught.  At Station two, four species of fish were captured.




Only one of each of these species was retained.  The species represented were




quilback carpsucker (Carpoides cyprinus), smallmouth bass (Hicropterus




dolomieui),  yellow perch (Perca flavescens), and minnows.  Station three




was represented by the following fish species; quilback carpsucker (Carpoides




cyprinus), largemouth bass (Micropterus salmoides), silver redhorse (Moxustoma




anisurum),  and minnows.  Minnows were the most abundant group represented.









Zooplankton




Fox River




     Except for June, Station one supported a considerably larger zooplankton




population than Station three (Table 5,6).  This was most evident in July




and August when Station one supported 161 and 188 org/1 respectively, compared




with Station three, which had only 34 org/1 in July and 17 org/1 in August.




Members of the Rotifera dominated the zooplankton present during the study.




The rotifera was most represented by Keratella cochlearis, Polyarthra vulgaris,







                                     10

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Keratella earlinae and Trichocerca sp.   Members belonging  to  the  order


copepoda were present In relatively low numbers.  The  copepoda, during  all


three months, were represented by species in the early form of development


(Cyclops Juveniles and Nauplii).





Wisconsin River


     Throughout the study period, Station one supported a  larger  zooplankton


population than Station three.  This difference was most noticeable in


June and July.  In June, Station one had a total of 139 org/1 compared  to


98 org/1 at Station three, while in July, Station one  contianed  65 org/1


compared to 34 org/1 at Station three.   In August, there was  only a slight


difference between Station one (28 org/1) and Station  three (24  org/1).


Members of the Rotifera dominated the zooplankton present during  the study.


The Rotifera were represented by Keratella cochlearis, Syncharta sp.,
                                                   • i

Trichocerca similis, and Polyarthra vulgaris.  The Copepoda were represented


by early life stages of species (Cyclops juveniles and Nauplii).   These


species, however, never became numerically important.





Phytoplankton


Fox River

       *
     Throughout the entire study period, Station one supported a considerably


larger phytoplankton population than Stations two and  three  (Tables 7, 8 and 9)


In June, Station one was dominated by the blue-green algae, namely Anabaena sp. ,


and Aphanizomenon flos aquae.  However, later in the summer,  a noticeable


change in the algal population occurred.  By July, the flagellated algae


consisting of Cryptomonas sp., and other miscellaneous flagellate species


increased substantially from June (612 cells/ml to 5860 cells/ml), thus


accounting for the dominance by both the blue-green algae and flagellates.


                                    11

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By August, the flagellate population continued to Increase reaching a
concentration of 12,970 cells/ml.  The blue-green population had decreased
from a concentration of 7250 cells/ml in July to 1130 cells/ml in August.
Thus, in August the flagellate population dominated comprising 87.0 per
cent of the population.
     Station two showed shifts in population numbers and composition between
June and August.  In terms of cell concentration in June, Station two supported
a population of 5253 cells/ml.  However, by July, the numbers of phytoplankton
encountered decrease to 2940 cells/ml and by August to 2720 cells/ml.  During
this same time period, changes in the dominant' algal forms also changed.
In June, the centric diatoms consisting of Cyclotella spp., comprised 68%
of the species, however, by July the flagellated algae "became dominant (62%)
and this trend continued through the August study.
     Station three, unlike Station two, showed an increase in the phytoplankton
population from June until August.  In June, the algal cell concentration
was 2907 cells/ml.  By July, the algae had increased to 6230 cells/ml, and
in August, to 10,940 cells/ml.  With regard to species composition, the June
study showed a diverse population of all major groups of algae with no one
form showing complete dominance.  However, by July, the flagellated algae
(consisting of Cryptomonas sp_.and misc. flagellates) began to increase in
numbers and became the dominant algae.  This group continued to dominate in
August.


Wisconsin River
     Station one on the Wisconsin River showed shifts in species composition
between June and August.  In June, the algal population was dominated by the
blue-green alga, Aphanizomenon flos-aquae,  the flagellated algae comprised
of Cryptomonas erosa and miscellaneous flagellates  and the centric diatom
                                    12

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Melosira spp.  In July, diverse algal population existed with no  major


algal group showing complete dominance.   However, by August,  the  flagellated


algae had continued to increase, and along with the centric diatoms  became


the dominant algal forms.  The flagellated algae were largely comprised of


Cryptomonas ovata, Chlamydomonas sp.,  and miscellaneous forms.   The


centric diatoms were represented largely by Melosira spp.
                                  •

     Station two exhibited a population structure similar to  Station one.


Throughout the study, the flagellated algae and centric diatoms dominated.


The species represented were similar to those present at Station  one.


     Station three, during the study period, consistantly supported  a


larger phytoplankton population than Stations one and two. In terms of cell


concentration, phytoplankton numbers changed little between June  and August.


In June, 9486 cells/ml were present, compared to 9510 cells/ml in July  and


11,840 cells/ml in August.  Although cell concentration changed  little


during this time period, species composition increased.  In June, the flagellates,


centric diatoms, and blue-green algae dominated.  Species represented were


the same as were present at Station one.  By July, the centric diatoms


became less important and the flagellates and blue-green algae dominated.


In August, the blue-green population decreased substantially  and  flagellates


and centric diatoms became numerically important.  The green  algae also


began to increase in August being comprised predominantly of  Crucogenia


.quadrata and Ankistrodesmus falcatus.


     It should be noted that throughout the study, green particulate matter


visible to the naked eye was suspended in the water of the Wisconsin River.


At all sampling stations, biological examination of the water in June and


July did not show any unusual increase in any of the phytoplankton species


encountered.  In August, this green particulate matter continued  to  "be


present, and due to heavy winds at the time of sampling, a large  amount of

                                     13

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this material began to collect along the Eastern shoreline between




stations one and two.  Biologocal examination revealed the substance to




be the algal species Microcystis aeruginosa.   This species had reached




bloom conditions in August and the heavy winds were accumulating the




cells into a mat along the windward shoreline.








Chlorophyll




Fox River




     Station one showed a substantial increase in the amount of chlorophyll a^




present in the water compared to Station three during the months of June




and July (Table 10).  This difference was most noticeable in the June survey




when Station one had an average chlorophyll a^ concentration of 47.5 yg/1,




compared to 9.13 yg/1 at Station three.  It should be noted that this difference




was probably due to the large number of phytoplankton cells observed suspended




in the water at Station one and in Swan Lake.  The algal population was




apparently approaching "bloom" conditions, although cell counts of water




samples did not show a "bloom" condition to be present.




     This large population of algae was not observed to be present in July,




accounting for the significantly lower chlorophyll a^ values obtained at




Station one during July.  However, there still remained a substantial




difference in the amount of algal biomass produced at Station One (15.9 yg/1)




compared to Station three (6.72 yg/1).




     In August however, unlike the previous two months, Station three showed




a substantial increase (25.1 yg/1) of chlorophyll £ present compared to that




at Station one (9.0 yg/1).
                                    14

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Wisconsin River




     Station three throughout the study period contained a greater amount




of algal biomass compared to Station one.   The most significant difference




occurred in June when Station three had an average chlorophyll a_ value




of 23.1 yg/1, while Station one had a value of 13.A yg/1.  This trend




continued during July and August, however, the differences between the two




stations was not as pronounced.  In July,  Station one had an average chlorophyll a_




value of 10.4 yg/1, while Station three had a concentration of 13.3 yg/1.   In




August, Station one had a value of 22.0 yg/1, while Station three had 27.7  yg/1




of chlorophyll a^ present.









Periphyton




Fox River




     Examination of the periphyton community in July showed Station one on




the Fox River to be more productive than Station two further downstream.




As one can see from Tables 11 and 12, Station one supported 23,298 cells/mm2




compared to only 5978 cells/mm2 at Station two.  Unfortunately, the periphyto-




meter at Station three was  either lost or stolen, thus no comparison between




the other stations could be made.




     With regard to species composition, Station one was dominated by the




blue-green algae, represented by Oscillatoria sp. and Coelosphaerium




kuetzingianum  and the pennate diatom Cocconeis pediculus.  Station two was




dominated by Oscillatoria sp. and Cocconeis pediculus.









Wisconsin River




     The periphyton community on the Wisconsin River showed Station one




to be less productive than either stations two or three.  Tables 11 and 12




shows that in July, Station one supported 221 cells/mm2, Station two





                                    15

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4928 cells/mm2 and Station three an average of 4948 cells/mm2.


     Species composition showed Station one to be dominated by  the centric


diatom Melosira spp.  Station two was dominated by Oscillatoria sp.,  and


Cocconeis pediculus.  Station three was best represented by the blue-green


algal species Aphanizomenon flos-aquae and Oscillatoria sp., and by the


pennate diatom Cocconeis pediculus.





Macroinvertebrates


Fox River


     The results of the qualitative and quantitative macroinvertebrate


samples are contained in Table 13. Station one data showed a great diversity

                                                                           0^.
of organisms in the qualitative samples for all months sampled  (June - 24 taxa,


July 24 taxa, August - 37 taxa).  For the most part, these organisms were


associated with the stream margins, in and among the rooted vegetation.


     Quantitative samples indicated habitation of the bottom sediments by


a highly diverse population in early summer (31 different taxa  in June) and


moderate diversity in July and August, with 19 and 20 taxa respectively.


     The population at Station one showed a mixture of pollution tolerant,


facultative and intolerant forms.


     Station two had a high diveristy in the qualitative sample for July


(43 taxa) and a lower diversity in August (12 taxa).  Ponar samples showed


a decrease in diversity as the summer progressed.  This was most likely


due to the decrease in dissolved oxygen and an increase in water temperature.


Also, the intolerant forms were not found in the August samples, as they


had been in June and July.




                                    16

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     Taxa diveristy decreased at Station three during the summer months.




The quantitative samples had 45 taxa in June,  20 taxa in July,  and 15 taxa




in August.  Here again, only the tolerant organisms were encountered  at the




end of the summer.  The qualitative samples had a high diversity of organisms




collected from among the abundant aquatic vegetation and along  the margins




of the stream.  The pollution intolerant forms found thorughout the summer




were for the most part, those organisms which live at the water - air




interface, and on the vegetation near the surface of the water  (i.e., -




Helisoma limosa and Anmicola integra).









Wisconsin River




     Table 13 contains the qualitative  and quantitative macroinvertebrate




data collected for the Wisconsin River  during June, July and August.   The




quantitative data for all stations, for all months, exhibited low diversity




and low total numbers of individuals.  The moderately fast river current




and substrate composition (a mixture of fine to coarse sand) are- responsible




for the reduction of the organisms, since such conditions greatly inhibit




perminant colonization of the bottom sediment by the macroinvertebrate groups.




     The extensive colonization of the  artificial substrate periphyton




samples and heavy utilization of overhanging trees and submerged vegetation




by organisms indicates that where the shifting sand substrate can be avoided,




a diverse population of individuals  (as high as 34 taxa at Station three in




July) can develop.  The decrease in the number of taxa for the month of




August on the Wisconsin River is probably due to the drastic drop in the




water level, which exposed large sections of previously inhabited areas




of the river bed.






                                     17

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     The raacroinvertebrate populations at all''stations  were  a mixture  of




pollution tolerant, facultative, and intolerant forms.   This existed through-




out the summer.









                               DISCUSSION




Station 1




     When comparing Station one on'the Fox River to that on  the Wisconsin




River, it appears that during the study period, the Fox River is biologically




more productive than the Wisconsin River.  This is supported by the fact




that during the entire study period, phytoplankton cell concentrations were




substantionally greater in the Fox River than the Wisconsin  River.   In June,




the Fox River supported 9741 cells/ml, in July, 14,300  cells/ml and in August,




14,910 cells/ml.  This compares to Station one on the Wisconsin River, which




in June, produced only 7344 cells/ml, in July 5680 cells/ml  and in August,




8679 cells/ml.  Examination of the periphyton community present during the




July study, further supports the fact that Station one  on the Fox River is




more productive than that on the Wisconsin River.  The  Fox River supported




23,298 cells/mm2 compared with only 221 cells/mm2 on the Wisconsin River.




     Except for August, chlorophyll ji concentrations showed  greater




productivity in the Fox River.  Zooplankton populations (except in June)




were also considerably greater in the Fox River.




     It should be noted that in July and August, several species of rotifers




were present at Station one on both the Fox and Wisconsin Rivers, which are




indicators of eutrophic conditions.  Although these species  were present in




relatively low numbers, their presence may indicate that these stations are




approaching eutrophic conditions.  The species represented were Brachionus




angularis, Keratella cochlearis, P_. tecta, Trichocerca multicrinis, Filinia





longiseta and Pompholyx sulcata.





                                     18

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     In.terms of macroinvertebrate findings, Station one qualitative samples


from the Wisconsin River had a lower diversity of taxa .(15)  as compared to


the Fox River (29).  Quantitative substrate samples also showed the Fox


River at Station one to be more productive than the Wisconsin River for the


same station.  Both rivers at this location had benthic communities composed


of tolerant, facultative and intolerant forms, which was characteristic

                                  •
throughout the summer.


     Based upon the biological parameters measured at Station one,  the Fox


River was classified as mesotrphic to eutrophic in nature, while the


Wisconsin River was classified as being mesotrophic.




Station 2


     Station two on he Fox and Wisconsin Rivers, for most of the summer,


appear to be similar in terms of phytoplankton productivity.  In June and


July, both rivers supported approximatley the same concentration of


phytoplankton.  The Fox River supported 5253 cells/ml in June and 2940


cells/ml in July compared to the Wisconsin River which supported 6018 cells/ml


in June and 3830 cells/ml in July.  In August, however, the  Wisconsin River


supported a considerably greater phytoplankton population (9560 cells/ml)


compared to that in the Fox River (2729 cells/ml).


     A similarity, during June and July, between Station two on the Fox


and Wisconsin Rivers is further supported by examination of  the periphyton


population.  Both stations supported approximately equal numbers of organisms.


The Wisconsin River supported 4928 cells/mm2 compared to 5978 cells/mm2 on


the Fox River.


     Fewer macroinertebrate taxa were encountered on the Fox River, Station


two (average of 23 taxa in the qualitative samples) as compared to the same


station on the Wisconsin River (29 taxa in the qualitative samples).  As the


                                    19

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summer progressed, the biological quality of the benthis community on the




Fox River at Station two deteriorated toward a greater, number of pollution




tolerant forms, while the Wisconsin River remained relatively constant.




     The overall condition of Station two was considered to be eutrophic




to mesotrophic for the Fox River and mesotrophic for the Wisconsin River.








Station 3




     In June and July, the Wisconsin River supported a considerably greater




pollution enriched biological community than the Fox River.  This is




substantiated by the fact that the phytoplankton population in the Wisconsin




River in June reached 9A85 cells/ml compared to only 2907 cells/ml in the




Fox River.  Chlorophyll ji concentrations in the Wisconsin River (23.1 vg/1)




and the Fox River (9.1 vg/1) also supported this evidence.  The Wisconsin




River also supported a much greater zooplankton population (98 org/1) than




the Fox River (19 org/1).  In July, the phytoplankton and chlorophyll ji




also showed greater biological productivity in the Wisconsin River, while




both rivers supported similar zooplankton populations.
                                    20

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Table 1   Chemical and physical analysis of water collected from the
          Fox and Wisconsin Rivers  June, July and August,  1978.
%
1
w .
0)
3l
>>
>
•-1
3
•-»
^
0)
|
CO
4J
0)
t>0
3
Parameter '
Temp. °C
Dissolved
Oxygen mg/1
PH
Specific
conductance
V ohms /cm
Temp. °C
Dissolved
Oxygen
(mg/1)
PH
Specific
conductance
(y ohms /cm1)
Temp. °C
Dissolved
oxygen
mg/1
PH
Specific
conductance
uohms/cm
Fox River
Station
1 ' • 2 ' 3
23.5
12.2-14.4
8.9
560
23.5
8.4
8.0
360
23.9
5.9
—
400
17.0
7.0
7.8
. 600
21.5-22.0
3.7-3.8
6.9
400
21.0
2.7
—
400
1.6.0
2.2
7.6
600
20.5
2.2
8.25
400
23.4
3.90
7.25
400
Wisconsin River
Station
1 '2 ' 3
17.0
8.4
7.4
160
23.0-24.0
7.45-9.6
7.6
140-156
26-26.3
8.75-8.80
7.7-8.0
150
21.0
8.4
7.4
160
20.5-21.0
6.8-7.4
7.5-7.55
150-160
24-24.5
4
7.6-8.3
7.5-7.75
150
17. .0
8.4
7.4
160
21.0-22.0
6.6-7.5
7.1-7.5
160
23.0
7.05-7.3
—
160

-------
Table 2    Length and weight measurements of fish captured in the
           Wisconsin River during August Survey 1978.
Station 1
Species
 Number
Captured
    Weight
(range in oz.)
    Length
(range in inches)
Carpoides cyprinus
(Quilback carpsucker)
Minnows
1
3
64.0
—
16.5
1.0-2.5
Station 2
Species
 Number
    Weight            Length
Carpoides cyprinus
(Quilback carpsucker)
Micropterus dolomeiui
(Smallmouth bass)
Minnows
Perca flavescens
yellow perch
1
1
1
1
64.0
16.0
—
8.0
16.0
9.5
3.0
6.75
Station 3
Species
 Number           Weight            Length
Captured	(range in oz.)	(range in inches)
Carpoides cyprinus
(Quilback carpsucker)
Micropterus salmoides
(Largemouth bass)
Moxostomo anisurum
(Silver redhorse)
Minnows
2
1
1
5
7.0
9.5
40.0
—
5.5-5.75
8.5
15.5
2.0-3.25

-------
Table 3    Length and weight measurements of fish-captured in the
           Fox River during August Survey 1978.
Station 1 *
Species
 Number
Captured
     Weight
(range in  oz.)
   length
(range in inches)
Amia calva
(Bow fin)
Lepomis cyanellus
(Green sunfish)
Ictalurus nebulosus
(Brown bullhead)
Lepomis microlophus
(Redear sunfish)
Perca flavescens
(yellow perch)
Sunfish
(young of the year)
1
1
1
A
1
15
9.0
2.0
9.0
—

—
7.5
3.5
7.5
2.75-3.50

—
   * Due to a malfunctioning of the Electroshocker, no fish samples
     were taken at Stations 2 and 3.

-------
Table 4
Fish species collected by electroshocking on the Fox
and Wisconsin Rivers during August 1978 survey.
Species Fox River
Station
123
Amia calva
(Bowfin)
Carpoides cyprinus
(Quilback carpsucker)
Lepomis cyanellus
(Green sunfish)
Lepomis microlophus
(Redear sunfish)
Micropterus dolomieui
(Smallraouth bass)
Micropterus salmoides
(Largemouth bass)
Moxostoma anisurum
(Silver redhorse)
Ictalurus nebulosus
(Brown bullhead)
Perca flavescens
(Yellow perch)
Stizostedion vitreum
(Walleye)
Minnows
(Unidentifed)
Sunfish
(Young of the year)
X

X
X



X
X


X

N
0

s
A
M
p
L
E




N
0

S
A
M
P
L
E



Wisconsin River
Station
12 3

X








X


X


X




X
X


X



;x
X



X


-------
Table 5   Zooplankton in organisms/1 collected from the Fox and Wisconsin
          Rivers in June, July and August 1978.
Species
Rotifera
Asplanchna priodonta
Brachionus angularis
Chromogaster ovalis
Colurella sp.
Conochilus unicornis
Euchlanis sp.
Filinia longiseta
Kellicottia bostonensis
Kellicottia longispina
Keratella cochlearis
Keratella cochlearis
v -tecta
Keratella crassa
Keratella carlinae
Monostyla lunaris
Monostyla quadridentata
Monostyla sp.
Polyarthra malor
Polyarthra romata
Polyarthra vulgaris
Polyarthra sp.
Pompholyx sulcata
Synchaeta sp.
Trichocerca multicrinis
• Trichocerca rousseleti
Trichocerca sirailis
Trichocerca sp.
Trichotria tetractis
Unid. spp.
OG
CLADOCERA
Clydorus sphaericus
Eubosmina coregoni
COPEPODA
Cyclops juveniles
Cyclops sp.
DiajJtomus juveniles
Nauplii
Fox
1










5




















2




2
2
June Survey
R. Wise. R.
Station No.
313






8



2




2
2





2





2


















2
36





2

7



81

8












3










27





2

5



53

2







2




5
July Survey
Fox R. Wise. R.
Station No.
1 | 3 113



2
8
2
2



75


23




8
19

3
5






6




3


5





2
2



6



3




3







3
6








9





2


2

14
2
2
3
2



2
8


5



5

2
2

3





6




2


2


6


2
2



2
3


8














5
Fox
1

2

5



2


105

6
9



6



2

5


19

5








22
August Survey
R. Wise. R.
Station No.
3 1-3






2











3



5











2
2

3


2







12
2




2





2
2
2



2








2


2







16



2

2

2




















-------
Table 6   Major zooplankton groups collected from the Fox and
          Wisconsin Rivers in June, July and August 1978.'
5-»
I
to
0)
§ '
•-}
Js
u
g
to
£
3
>->
^
0)
&
3
CO
*J
(0
o
to
3
Species
Rotifers
Cladocera
Copepods
Total
Rotifers
Cladocera
Copepods
Total
Rotifers
Cladocera
Copepods
Total
Fox
Sta
1
5
2
A
11
153
—
8
161
166
—
22
188
River
org/1
tion No.
3
18
—
—
18
25
—
9
34
10
—
7
17













Wiscon
0
Sta
1
136
—
3
139
56
3
6
65
26
—
2
28
sin River
rg/1
:ion No.
3 •
91
2
5
98
29
—
5
34
24


24

-------
Table 7        Phy'toplankton in cells/ml collected from the Fox and Wisconsin Rivers
               in June, July and August 1978.
June Survey
Fox River VJisc. River
Station No. Station No.
Esc-cies '• 1 ' 2 ' 3 ' 1 ' 2a ' 2c •' 3
BLUE-GREEN ALGAE
Agmenellura sp.
Anabaena sp.
Anacystis cynea
Anacystls sp.
Aphanizomenon flos-aquaa
Chroococcus sp.

Coelosohaerium
Coelospharium sp.
Lyngbva maruensiana
Microcystis aeruginosa
Oscillatoria limnctlca
Oscillatoria sp.
GREEN ALGAE
Actinastrium hantzschil
Actinastrium sp.
Ankistrodesraus falcatus

Closterium sp.
Coe last rum microporum
Coe last rum sp.
Cosmarium sp.
Crucigenla quadrata
Crucigenla tetrapedia
Crucieenia sp.
Dictyosphaerium pulchellum
Dictyosphaerium sp.
Colenkinia sp.
Kirchneriella sp.
Micractinium sp.
Oocystis 'sp.
Pediastrum boryanum
Quadrigula laqustris 	 ,


5610


3315






























51


204







153

























51











204


51




102
51





51

51

51


2142





51





204







102






51



102


510







51



153














153



51


714







51



153


51




102
51





102



51


1938







51

51

357



51










153

July Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3


90
290

5850
90

90




90


















30




90

260


60




110





30






30

60


90



110

30

140
90

110




230



110





170



30
30


110
30


230
30
110
30
520


90








490





350
60







30


110

200

290
30






30


30
170







140




60




90

200

170







30



260





170








30


60
30
230

3160
140

30












110
30

230





30
30


August Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3


70
140

830


30

30

30


















140





30

100







100

















70



1100

280

280







210


210
1040



70

140







350



240
30
210

70











410





210


30



30
70



100
30
720

100


70




210



140





140





100

30
30








N
0








S
A
M
P
L
E












240

450

30


30



100



70
550



30

720







30



-------
   Table 7

1   cont'd
Phytoplankton in cells/ml collected from the Fox and Wisconsin Rivers
in June, July and August 1978.
June Survey
Fox River Wise. River
Station No. Station No.
Species ' 1 ' 2 ' 3 ' 1 ' 2a ' 2c • ' .3
Scenedesmus abundans
Scenedesmus bijuga
Scenedesmus quadricauda
Scenedesmus dimorphus
Scenedesmus sp.
Schroederia setigera
Selenastrum sp'.
Tetraedron caudatum
Tetraedron minimum
Tetraedron sp.
Tetrastrum staurogen-
laerorme
Tetrastrum sp.
Misc. greens
FLAGELLATES
Ceratium hirundin.ella
Chlamydomonas sp.
Cryptomonas erosa
Cryptomonas ovata
Cryptomonas sp.
•Eudorina sp.
Euglena sp.
Mallomonas sp.
Pandorina sp.
Phacus sp.
Trachelomonas sp.
Misc. flagellates
. CENTRIC DIATOMS
Cyclotella sp.
Melosira sp.
Microsiphona po tamos
Stephanodlscus sp.
. PENNATE DIATOMS
^Asterfonella formosa














51

51

408






102









51












51
102

51

51



51
612

3570






51
153








51




306

153
•




51
510

459
306




153

357













765





51


714

102
2244

51




153

51

153









663

403






510


2295

51




153

.153











459

408


51



612

204
2091 •

51

408


204
51





51

102




459

_765






1632

612
1683

102

816
July Survey
Vox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3





90











90
820


60



L940

30
430









30











60
410

30
30



1300

60
60






60


30

30

60





60
110
230
430

30
30
30
30

2260

110
1160




60

30

30
110
30








30
170
230
290


30

30

1480

30
1040
90
30


60




60
60

30



110



90
350
290






1100

110
670

60


30'

60

30
110



30






90
350
460




60

750

110
670
30





60

60
230











350
350






3010

90
1100
30



August Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a 2c ' 3


30


30








280

70
70
240

30
30



12250

30
140






70














30


30




1860

100
30

30




70

70
30


70

70




410

140
350

30


30
30
4140

550
550
690





30

70
30



30
100




410

410



30


30
2760

280
2760
100
100




70

140




30






30
70
140

60
30



3100

310
2860
790
30





































170

140
70




30




270
410
310
210
30
30
70

30

2970

1000
1690
1860




-------
Table  7
cont'd
Phytoplnnkton in cells/ml collected from die Fox and Wisconsin Rivers
in June, July and August 1978.
June Survey
Fox Rivor Wise. Paver
Station No. Station No.
S-ccies ' 1 ' 2 ' 3 ' 1 2a ' 2c •
Cocconels sp.
Cymbella sp.
Diatoma sp .
Fragllarla crotonensls
Fragilaria construens
Gomphonema sp .
Navicula sp.
Niczschia acicularis
Nitzschla palea
Nitzschia sp.
Synedra acUs
Synedra -ulna
Synedra sp.
51







51
102




51





.
204
51



102



204




51











153
102







1020




51











102
/



3








204
153



July Survey
i'ox River VILuc. River
Station No. Station No.
1 ' 2 ! 3 ' 1 ' 2a ' 2c ' 3
30




30
30






30





30

110
90



• 110


30


60
30
60


90









30












30
30
30

















30



30


90



August Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3
30


310


100






30






30
210




70


210


70

70 '
210





30




30

140
30










100
100


30



















140
240
70

30
30


-------
Table 8     Major phytoplankton groups  collected  from the  Fox and
            Wisconsin Rivers in June, July and August 1978.
>*
0)
Sr
V)
Q>
§•
•->

>-,
*
M
3
M
>>
rH
3
•-}

^

-------
Table 9     Diatom species proportional count by  percent of  phytoplankton  collected  from
            the Fox and Wisconsin Divers in June,  July  and August  1978.
June Survey
Fox River Wise. River
Station No. Station No.
*-»c.i°-.3 ' 1 ' 2 ' 3 ' 1 ' 2a ' 2c •' 3
Achnathes sp.
Amphora sp.
Astertonella formosa
Cocconeis pedlculus
Cocconeis placentula
Cocconeis sp.
Cyclotella comta
Cyclotella glomerata
Cyclocella meneghiniand
Cyclotella stelllgera
Cyclotella sp.
Cymbella sp.
Di aroma tenua
v. elongocrum
Diatoma vulgare
Dlatoma sp.
Diploneis sp.
Epithemia sp .
Fragilarla capucina
Fragllarla crotonensls
Fragilaria construens
Fragllaria intermedia
Fragilaria leptostauron
Fragtlaria plnnata
Fragilaria sp.
Gomphonema olivaceum
Gomphonema sp.
Melosira ambigua
Meloslra dlatana
Melosira granulata
Meloslra longlsplna
Melosira islandica

2



6


3 .

25
2











42












2


90

2
<1






1




2

<1










16


20





3








15



27





11


<1


1
<1

<1
<1

2



1




3



it



<1
<1
18


<1


<1


<1


2



1




10



5





15


<1


<1


<1


<1

<1

3




2







<1

10





<1
<1




2








2



1



July Survey
•'ox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ! 1 ' 2a ' 2c ' 3



6


<1



<1
5




2

20

<1


2

5
43

5


1

<1
34
3



9

<1
<1


5



3
13




<1
<1
4






<1
17
<1



13

3
<1


3


3
3
4
6




<1
12
2



2


<1
<1



<1

<1
<1
<1

<1



2
2



2

<1
37
1



2

1
1




<1
1
1



1

<1

5
5



2

1
49
4

2

<1

2
<1
<1




<;!
1

-------
Table 9
              continued
June Survey
Fox River Wise. River
Station No. Station No.
Scecies ' 1 ' 2 ' 3 ' 1 ' 2a ' 2c • ' 3
Melosira italica
Melosira vnrians
Melosira sp.
Navicula sp.
Neidium sp.
Nitzschia acicularis
Nitzschia holsatlca
Nitzschia palea'
Nitzschia sp.
Pinnularia sp.
Rhoicosphenia curvata .
Stauroneis sp.
Stephanodlscus niagarae
Stephanodiscus sp.
Surirella sp.
Synedra acus
Synedra ulna
Synedra sp.
Tabellaria fenestrata
Thallosira fluviatills


13 •
2
2



2



4










3

<1










<1




11
3
3

<1






<1



2




<1
70
3

<1


1



1

<1






2
54
2

<1
10

<1



1

<1

<1




1
71
10

<1
2





1









-------
Table 10    Chlorophyll a^ concentrations of water collected from the
            Fox and Wisconsin Rivers in June, July and August  1978.
                      Chlorophyll ji (corrected for phaeophytin)
                                         ug/1
June
Survey

July
Survey

August
Survey
River
Fox River
Wisconsin R.

Fox River
Wisconsin R.

Fox River
Wisconsin R. '
* la '
48.8
13.9

16.40
10.92

8.4
18.90
Ib '
65.9
13.0

15.63
9.85

9.6
25.53
Ic '
28.0
13.2

15.58
10.54

9.0
21.60
3a 1
9.0
23.8

7.20
12.80

19.8
26.2
3b 1
9.6
19.8

5.19
13.67

33.2
27.8
3c
8.8
25.6

7.76
13.37

22.4
28.95
*  The letters a, b and c following the station numbers are used to
   denote substations which were taken along a transect across the
   river channel at that station.

-------
Table 11       Periphyton in cells/mm2 collected from the Fox and
               Wisconsin Rivers July 1978
Wisconsin River
Station
Species 1 ' 2 ' 3a ' 3c
Blue-Green algae
Agmenellum sp.
[ Anabaena sp.
Aphanizoraenon flos-aquae
Coelosphaerium kuetzingianum
Oscillatoria sp.
Greest Algae
Actinastrum sp.
Ankistrodesmus falcatus
Crosterium sp.
Pediastrum boryanura
Scenedesmus diraorphus
Scenedesmus falcatus
Scenedesmus quadricauda
Scenedesmus sp.
Stigeoclonium glomerata
Flagellates
Chrysococcus sp.
Cryptomonas ojrata
Chlamydomonas sp.
Cryptomonas crosa
Mallomonas sp.
Trachelomonas sp.
Misc. flagellates
Centric Diatoms
Cyclotella sp.
Melosira sp.
Pennate Diatoms
Asterionella formosa
Cocconeis sp.
Cymbella sp.
Gomphonema sp.
Navicula sp.
Nitzschia sp .
Snyedra ulna













5
3








31

5
122

8


3
34
10



24


3000


24
2

9
3
16
19


2





67


176


1380


57
86


25

1530

4300







3









12


60


628
21
521
18
34






86

<1
<1

<1








<1
9

<1
4


5


1304

1
2
3

Fox River
Station
1 1 ' 2 ' 3




2800
5950


14






70


28




218


252


13594

14
15
126





40
5450


<1


3

3





3
1
<1

94

4
31


336

<1
5
8
<1


N
0






S
A
M
P
L
E




















-------
Table 12   Diatom Species proportional count by percent of periphyton
           collected from the Fox and Wisconsin Rivers in July 1978.

                                     July Survey
                            Fox  River          Wisconsin River
                            .  Station              Station
Species '
Achnathes lanceolata
Achnathes sp.
Amphora sp.
Anomoeoneis sphaerophora
Asterienella formosa
Cocconeis pediculus
Cocconeis placentula
Cyclotella glomerata
Cyclotella meneghiana
Cyclotella sp.
Cymbella sp.
Diatoma tenue v. elongatum
Diatoma sp.
Epithemia sp.
Eunotia sp-
Fragilaria construens
Fragilaria crotonensis
Fragilaria intermedia
Fragilaria sp.
Gomphonema olivaceum
Gooiphonema sp.
Melosira ambigua
Melosira distans
Melosira italica
Melosira varians
Melosira sp.
Navicula sp .
Nitzschia acicularis
Nitzschia palea
Nitzschia sp.
Pinnularia sp.
Stephanodiscus binderanus
Stephanodiscus niagarae
Stephanodiscus sp.
Surirella angustata
Surirella sp.
Synedra ulna
Synedra sp.
Tabellaria fenestrata
Tabellaria fenestrata
V. geniculata
Thallosira fluviatilis
1 |



<1

98
<1



<1




<1
<1


<1






<1














2 1

<1



74
<1

<1
3
<1

<1


3

1

1




4

6
<1
<1
1


1 •
1



1



3



N
0



S
A
M
P
L
E



























1 ]
1
<1



79
1


1


1


3

1



3
1
4


2


<1


<1




2



2a ]



N
0



S
A
M
P
L
E



























2c |

<1


<1
77
<1

<1
3
<1
<1
<1
<1

1
<1
3

<1

<1
2
3
<1
3
1


<1

<1
<1




<1
<1
<1
<1
3
<1
<1



96
<1


<1



<1
<1



<1
<1

<1

<1
<1
<1

-------

June
                             Table  i_>
  Macrolnvcrtcbrate  Data  Far the Fox  River,  Pottage,  Wisconsin
Quulllnilvu  Sumj>lcs                                          Quantitative Sam[iloB
    July              August                 June                 July                August
123123           123123123

Ablabesmyla sp.
Oratopnp.CTit dae
Chlronoaus sp.
Clinotanypus sp.
Crlcotopus sp
Cryptochironomus sp
Diamesa sp
Dlcrotendlpes sp
Endochlronomus sp
Euklefferlella sp.
Clyptotendlpes sp
Goeldlchironomus sp
Harnlschia sp.
Kiefferulus sp.
Mlcropsectra sp
Microtendlpes sp.
Parachlronomus sp
Paratendlpes sp
Pentaneurlnl tribe
Phaenopsectra sp
Polypedllum sp


1
1

3
1

19
37

31

1



8
8


5

/
,
/
,
1 '
' ,
,
,
,
,
Cl
J£
a
(-<
01
a
CO
o
z
/
/
/
/
/
/
/





2


6
1

6





1

2
14
3









80

30





4







1




1
14

1





3

7

1

















1






32





12 .
132










204






1



3











1








1
1









2

13
























8
20
4

4

148
30

16

26
6
10

4
4
2

112



27

18

1
3
2







12

1

1

2

1

21


14
4

11





2
2

17
9


8
12


60

28


476
4

8










77


13


1

2

2



8

4

9





29





4





9



20


2



11

11
6

2







6







1
4

1
































4

2













































1
F
T
F
T

I
F
F

T




I
F


I-F
F
























-------
Table 1'J ccmc'd
  Macroinvertebrate Data For  the  Fox River,  Portuge, Wisconsin
Qualitative Suinpla                     '                      Quantitative! Sample
                                                                                               Cage i
June July August June
123123123 123
Dlptera
Procladiua ep
Psectrocladlus sp
Pseudochironomus sp
Slmulildae family
Sinmltldae pupae
Tanypus sp
Tanytarslnl tribe
Thienemannlella sp.
Tipulldae family
Tribelos sp.
Trlchocladius sp
Unidentified pupae
Unidentified sp









1





172














/
/
/
/
/
' e
0)
-^
Ta
H
V
a.
o
z
/
/
/
/
/










1




8




1
1















2




10










3




1
25




2













1


















12





20.




8






































2




2
































116





942




46
20









40
1



2



1


1









1
1




10




1
1








1

34





192



8
20
1








July
231

6





7

















4



















29





175




3









August
2 3

4










































Tolerance













































T-F
F
F-I
F

F






































-------
       Table 13 conC'd
                                                                                               Page 3
         Qunlltntlvo Sfimploii
   June              July
123123
   August
1      23
        QuonlUnUvc S;ira|i]i-a

 June                  July
12312
  August
1      2
                                                                                                                              Tolerance
TRICHOPTERA
AzruTlpn up.
Coracles up.
Cheuo^to psyche sp.
Cheumatopsyche sp. (pupae
Hydropsychldae
Hydropsyche sp.
Kydropsyche sp. (pupae)
Hydroptlla sp.
Hydroptllldae
Leptocerldae
Lcptocerus sp.
type sp.
Hlcrasema sp.
Nectopsyche sp.
Neurecllpsis sp.
Oecetis sp.
Polycentropidae
Serlcoscoma sp.
Thellopsyche sp.
Unidentified sp.


1


)
































•
















1





1










4










3



1
















1
3





























1









2

1

4

1

































3







1




























3














6





































It
1
















































10








It


































2













1
1


































































































1























-------
Table 13 cent Id
                                        Fox
                                                                                 Page  4
     Juno           July
12312
                         August
                 31      23
  June
12
     '     July
312
       August
312
                                                                                                             Tolerance
ODONATA
Apiichnn up.
Anax ap.
Coenagrlonidae
Ischnura sp.
Llbellulldae
Nehalennla Irene
Pe r 1 themua domi C la
Tetragoneurla ap.
Zygoptera
Unidentified sp.
EPHEMEROPTERA
Baetidae
Caenis sp.
Cent rope Hum sp.
Ephoron sp.
Heptagenildae
Hexagenla llmbala
Hexagenia sp.
'Neoephemera blcolor
Siphlonurus sp.




1



1


















- ••


























1














1
1
1





















10








































2


64



5




8












3





















9





1








2


1























































3











1









2







































1









2












4



















2

















1
1































































































































-------
Table 13 conc'd
          Quantitative  Samples
                                    Fox
                                                                                                    Page }
Quantitative  Samples
June July August June ' July August
123123123 1 2 3 1 2 3 1 ! 3 iol««,c«
EPHEMEROPTERA
fit pniinrran B|>
Unidentified up

. COLEOPTERA
Berosus sp
Coptotoaus sp
Dlneutus sp
Dublraphla sp.
Enochrus sp
Cyrlnidae
Gyrlnus sp
Hallplus sp
Hydrophllidae
Hydroporlnae sp
Laccophllus sp
Peltodyces sp
Unidentified sp






























....













































1
2




























1
1
1












1








5

















3
















30




1











1




12

1

43



1
7

























1











2


















1












































































































3























































1

























































































-------
Tablo 13 cunc'd
                            Fox
                                                                                        Page 6
  Qualitative
                                                               Quantitative
June July August June . July August
1231231 23 1 231 2 31 2 3 Tolc
HEHIPTERA
Abedus sp
Corixldae
Lethocerus sp
Hesovellidae
Notonecta sp
Pelocorls sp.
Plea striola
Ranatra sp.
Slgara sp.
' LEPIDOPTERA
Pyralidldae

COLLE.MBOIA
Isotoma sp.
Isotomurus palustrls
































..'..,









































8




















4
1


2
6







































1


3

2 .
5


4


































3







































1































































































































































i






























i





























ranT*fi



































































-------
Table 13  conc'd
                                                                                          Page 7
Quill 1 1« tl vi- SIIIU|>UMI Qii/mtUnttvu Snn:|ili!M
June July August . Juno • July August
Species 123123123 123123123 Tolerance
ISOPODA
A«»pl luq up.
A. racovltzai
ClADOCERA
Alona sp.
Daphnla galeata
D. juveniles
D. 'pulex
D. ap.
Eubosmlna coregonl
Eurycercus lamellatus
Leptadora klndtil
Slda crystalllna
Slmocephalus serrulat
Unidentified sp.

































.- ,
"•'









-








5







2

2
8
















8





















14


2


18









3





1





12















2





































47














































2


















6


5


















19











3











4



40





















10





4









16











3





















6













































































































































-------
Table 13 cont'd
                                                                              Page 8
   Quiintl tntJ vi- SnmploH
                                                                Quolltntivr
June July Auguxt June July August
123123123 123123123
AMFHIPODA
CnTnn.irufl «p.
Hyalella ap.
COPEPODA
Copepoda
Cyclopoida
Cyclops bicuspldatus
PELECYPODA
Muscullnum lacustre
Sphaerildae
GASTROPODA
Amnlcola Integra
Acmicola llmosa
Annicola sp.
Campeloma declsa
Cyraulus hirsutus
Gyraulus parvus
Uellsoma trivolvls






205













78





























38






2

53




6
25






51













70







252








6



32
15
63






5


3





10





1






303













2





























39






3

1
53


2
6





























a

4



















H

36




2


2











21





It
2

95
2




13






56





















21


1









4

4




























70





















2










1

















3












Tolerance

























































































-------
           Table  i'i cone'a
          Qualitative Sum|>U'!t
  June               July         •    August
1231231       23
          Quantitative S.'im.ile
  June             '   July
12312
        August
312
                                                                                                                             Tolerance
GASTROPODA (cont'd)
Hydrnbl.i nick) Inland
Lynnaca sp.
Physa elllptlca
Physa Integra
Physldae
Planorbldae
Pleurocera acuta
Pleurocera sp.
Promenetus exacuous
Valvata sp.
Valvata tricarnata
Unidentified sp.
OTHER
BRYOZOA
TURBELLARIA
Cura foremanlt
COELENTERATA
Hydra sp.
Hydracarina sp.
HEXATODA





13






1






4







.- ,





















14




2

2












9

27






2






1





1

23






3






5





2

3





















2





1



INC




1






















3





1



1
15

3


















































1

15


1


5




22

3






1

8





44






37




64

23

2





















76






2












4

46













5







21





















24





















14












2








6


























































































-------
                Talbc  13  cont'd
                                                                                   Page  10
Quail tn[ lv« SumplciH
June July AuguHt June
123123123 12
OTHER (cont'd)
l.TMATOMORI'HA
Paragordlus sp.
TURBELLARIA
Cura foreoanti

Total eaxa





.













54

26





















NS








•












33



















6

24








.-










35

43



















8

22



















123

37



















1

12



















2

37









































2

31



















74

31















3






45















July AuEunt
123123 Tolcrancd




36

19



















60

30



















124

20



















18

20



















24

17



















12

15















































1























































-------
Table 14
Qualitative and Quantitative Macrolnvertebratc Date Collected from tho
iUver during June, July and August, 1978 ac Portage, Wisconsin
                                                                                     Wisconsin
              Qualitative Samples
                                                               Quantitative  Samples
Month June July AuguHt June July August
Station 123123123 123123123 Tolerance
UIPTERA Ablabesmyla
Ornf-poj.rinld.-ie
ChLronomus sp.
Cllnotanypus sp.
Crlcotopus sp.
Cryptochlronoous sp.
Dlamesa sp.
Dlrotendlpes ap.
Endochlronomus sp.
Eukiefferiella sp.
Clyptotendlpes sp.
Gpeldlchlronomus sp.
Harnlschla sp.
Klefferulus sp.
Mlcropsectra sp.
Mlcrotendlpes sp.
Parachlronomus sp.
Paratendlpes sp.
Pentaneurlnl tribe
Phaenopsectra sp.
Polypedllum sp.

sp.

2

2


48
2

82
1



2
9



34



8

-8 -



88

112




•
24



2



-

1

1
6
2







4


2
12







13



4









9



1


1

1
12
5

1




1



72





27



2
8






1



90


























































































7





















7



1













2
8


15
2

3















7


3





















8





















18






1











1

9





















6





















33

































































I
F
T
F
T

I
t
f

T



'
I
F


F-I
F
























-------
     Table 14
Si   corvt'd
                 Qualitative  Snmples
                                                                             Quantitative Snrnples
                                                                                                               August
123123123 123123
Dlptera (cont'd)
fr- ln.ll.,.. .,..
Pscc t rocladtus sp.
Pseudochironomus sp.
Slmullldae family
Slmullldae pupae
Tanypus sp.
Tanytarslnl tribe
Thlenemannlella sp.
Tlpulldae family
Tribelos sp.
Trichocladlus sp.
Unidentified pupae
Unidentified sp.







1



1



6





9












'- -








4











1









l'












147
7

3


















1
2

5
1

1

1













385
3

12
4


1
2
1















6













































1































































3

3



1












1








1











































4












1

















1 ° 2









1





















1












3













1































Tolerance •























T-K
F
f-l
F

F






































-------
Tnble  14 cont'd
n
             Qualitative  Suraplus
   June               July
1     2      3     123
  August
1      2
            Quantitative S-'imp
  June                July
12312
  August
12
                                                                                                                            Tolerance
TRICHOPTERA
Chi'um.'itiipMyclur H|».
Cheumatopsyche sp.(pi
Hydropsychidae
Hydropsyche sp.
Hydropsyche sp.(pupa<
Hydroptlla sp.
Hydropttlldae
Leptocerldae
Leptocerus sp.
type sp.
Mlcrasema sp.
Ncctopsyche sp.
Neurecllpsls sp.
Oecetis sp.
Polycentropidae
Sericoscoma sp.
Theliopayche sp.
Unidentified sp.




20
pae)


)







2












•- -


'


























a




i





420
27

70
3












1




198
4

25








1




1




147
20

85


3
1




2









25



15





1



2


2


























3










1






















































1

5

2

















2
























4








































2


3






























































4

































































T


F




F



































-------
Tnole 1<*   cone 'd
Qualitative Samples Quflntl tntlva Snmplvn
June July August June July August
1231231 23 1 231 2 312 3
ODONATA
Arnrhn.'t u|).
Anax sp.
Coenagrlonldae
Ischnura sp.
Llbellulldae
Nehalennla Irene
• Perlthemus domic la
Tetragoneurla sp.
Zygoptera
Unldentlfed sp.
EPHEMEROPTERA
Baetldae
Caenls sp.
Centroptilum sp.
Ephoron sp.
Heptagenlldae
Hexagenla llmbala
Hexagenla sp.
Neoephemera blcolor
Slphlcmurus sp.

















3

















1




1













1


























26





















39



1













1



33



































1 '































































































1
































































































































































Tolennce

























































P

I





























-------
        Table 14  cont'd
        04
           Qua 1lintIvo Sntnploa
   June              July             August
1231231      23
         Quantitative Siunplc
   June               July
1      2312
         August
3     12
                                                                                                                             Tolerance
EPHEMEROPTERA
Stcnon<*aA fip.
Unidentified sp.
COLEOPTERA
Berosus sp.
Coptotomus sp.
Dlneutus sp.
Dablraphla sp.
Enochrus sp.
Cyrlnldae
Cyrinus sp.
Hallplus sp.
Hydrophilldae
Hydroporinae sp.
Laccophllus sp.
Peltodytes sp.
Unldentlfed sp.







1




















1

-2 -
":-
1








•
1




1









2
2
1
1

































1



3

















1
































































3


























































































































































2







































































































































F



T

T









!



















1 '

-------
       Table 14  cont'd
             Qualitative  Samples
    June             July    .        August
1231231      23
        Quantitative Samples
   June           '    July               August
1      231      2      31      2      3        Tolerance
HEMtPTERA
Abr:r1im tip.
Corlxldac
Lethocerus sp.
Mesovellldae
Notonecta sp.
Pelocorls sp.
Plea strlola
Ranatra sp.
Sigara sp.

LEPIDOPTERA
Pyralldldae

COLLEMBOLA
Isotoma sp.
IsotoD&rus palustris





















1







7

.' ,.
'•'


















45
1
1



















3




























65





















70






































































































1





















2




























5






























































































































































































F

























i















-------
Table 14   cont'd
        Qualitative Samples
Quantitative Samples
June July August June
123123123 12
OLICOCHEATA
Dcrti H|>.
Llmnodrllua sp.
Naldidae
Nals sp.
Peloscolex sp.
Stylarla laeustrls
Unidentified
HIRUDINEA
Batracobdella paludo
B. phalera
Dlna microstoma
Dlna parva
Eropbdellidae
Closslphonla complan
G. heterocllta
Helobdella elongate
B. fusca
U. llneata
U. pap 1 Hat a











ia




ita










61
- •


33


















9




















5




















IB
1



1
















1
69



10






1
































































































1























1

1

2














3




1


1














July August
1 2 3 12














































































































3






















Tolerance














































T

r






T
T

T






























-------
Table 14 conc'd
n
Qualitative S.implun Quantltutlvu Sample*
June July August June • July August
123123123 123123123 Tolerance
H. stagnalls
II. up.
llllnobdclla sp.
Piscicolldae
Placobdella ornate
Unidentlfed ep.





















































•








































































































































































































































































































































































































































T












































-------
   08
         Qualitative Samples
June             July             August
      Quantitative S.-iraplcs
June            '   July
                                      August

ISOPODA
A'ifl ItiM «p.
A. racoviczal

• CLADOCERA
Daphnla galeata
0. Juveniles
0. pulex
D. sp.
Eubosinina coregonl
Eurycercus lanellati
Leptodora kind til
Slda crystalllna
Sinocephnlus 'serrula
Unidentified







1










s


ta
3







2


12





77


2










3


6





16


3










1






















2


2




1


2











3

1



3


5













1






















2






















3













































1





12
.6


2












2








11


1










3








25


3










1






















z






















J








1



1

2







1






















2






















3







































































P











































-------
         Table 14 conc'd
         09
       Qualitative Samples
June            July
 23123
August
          Quantitative Sampl
  June                July
12312
                                                               August
                                                       3123
                                                                                                                    Tolerance
AMPHIPODA
G.'irrcnnrim np.
Hyalella azteca
COPEPODA
Cyclopolda
Cyclops blcuspldatuE
PELECYPODA
Muscullun lacustre
Sphaerlidae
GASTROPODA
Amnlcola Integra
Amnlcola linosa
Acnlcola sp.
Carapeloma decisa
Cyraulus hirsutus
. Gyraulus parvus
Helisoraa crivolvls






3






















1>

•• -









2









27





3




17
































2





















7

1
1











































































7























. ;



















































































'












































































3














•







































































F
F







I
I
I
1
P

F




























-------
       Toblo 14 cont'd
       »10
Qualitative Samples
Quantitative Samples
June July August June • July August
123123123 123123123
GASTROPODA (cont'd)
Hydrobla nlckllniana
Lymnaea sp.
Physa clllptica
Physa Integra
Physldae
Planorbldae
Pleurocera acuta
Pleurocera sp.
Promenetus exacuous
Valvata sp.
Valvata trlcarlnata
Unldentifles sp.
OTHER
BRYOZOA
.TURBELLARIA
Cura foremanll
COELENTERATA
Hydra sp.
Bydracarina sp.
NEMATODA





4













9







30 -













2
1





10


1
169








12

i

i





5





































1









1

































































21























































4





















6


















































































































































































Tolerance
























F

T-F


F



I


































-------
r
                                                                    Table  14 cone*d
                                                                    011

                                                           Quulltntlvc Samples
Quantitative Samplea
June July August June July
123123123 123123
OTHER (cont'd)
NF>tATOMOfirilA
ParagordluB sp.
TVRBELIARIA
Cura foreman.il

Total Taxa



















37

22



















•2 -

25





















32



















3

16



















3

33



















3

34





















6





















NS





















6











































7

















1



19





















12





















2





















2





















10















August
123 Tolerance






2





















3





















3





































































1

































-------