vvEPA
United States
Environmental Protection
Agency
Industrial Environmental Research EPA-600/7-80-079
Laboratory April 1980
Research Triangle Park NC 27711
Level 1 Biological
Testing Assessment and
Data Formatting
Interagency
Energy/Environment
R&D Program Report
-------
RESEARCH REPORTING SERIES
Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology. Elimination of traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:
1. Environmental Health Effects Research
2. Environmental Protection Technology
3. Ecological Research
4. Environmental Monitoring
5. Socioeconomic Environmental Studies
6. Scientific and Technical Assessment Reports (STAR)
7. Interagency Energy-Environment Research and Development
8. "Special" Reports
9. Miscellaneous Reports
This report has been assigned to the INTERAGENCY ENERGY-ENVIRONMENT
RESEARCH AND DEVELOPMENT series Reports in this series result from the
effort funded under the 17-agency Federal Energy/Environment Research and
Development Program. These studies relate to EPA's mission to protect the public
health and welfare from adverse effects of pollutants associated with energy sys-
tems. The goal of the Program is to assure the rapid development of domestic
energy supplies in an environmentally-compatible manner by providing the nec-
essary environmental data and control technology. Investigations include analy-
ses of the transport of energy-related pollutants and their health and ecological
effects; assessments of, and development of, control technologies for energy
systems; and integrated assessments of a wide range of energy-related environ-
mental issues.
EPA REVIEW NOTICE
This report has been reviewed by the participating Federal Agencies, and approved
for publication. Approval does not signify that the contents necessarily reflect
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This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.
-------
EPA-600/7-80-079
April 1980
Level 1 Biological Testing
Assessment and Data Formatting
by
David J. Brusick
Litton Bionetics, Inc
5516 Nicholson Lane
Kensington, Maryland 20795
Contract No. 68-02-2681
Task No. 401
Program Element No INE623
EPA Project Officer: Raymond G. Merrill
Industrial Environmental Research Laboratory
Office of Environmental Engineering and Technology
Research Triangle Park, NC 27711
Prepared for
U.S. ENVIRONMENTAL PROTECTION AGENCY
Office of Research and Development
Washington, DC 20460
-------
ABSTRACT
A scheme was developed for the comparison of Health Effect and Ecological
Bioassay Assessment Data. The scheme was based on the assumption that
each test method had a maximum dose (concentration) which could be reli-
ably applied and that effects based on fractions of the maximum applicable
dose (MAD) can be designated as degrees of toxicity. The levels of
toxicity based on fractions of the MAD are given as high, moderate, low,
and nondetectable (i.e no effect detected at the MAD).
Employing this scheme, data from three pilot studies including water
effluent samples, fluidized bed combusion samples, and coal gasification
samples were examined.
n
-------
TABLE OF CONTENTS
Page No.
ABSTRACT ii
LIST OF FIGURES iv
LIST OF TABLES iv
I. INTRODUCTION 1
II. DEFINITION OF HIGH, MEDIUM, LOW, AND NONDETECTABLE
TOXICITY 2
III. DATA TRANSITION FORMS 4
IV. ABBREVIATIONS USED 13
V. APPLICATION OF THE MAD CONCEPT FOR ASSIGNMENT OF
TOXIC LEVELS IN PILOT STUDIES 14
VI. DATA ORIGIN 28
VII. AMES TEST DATA INTERPRETATION CRITERIA 29
VIII. DATA CONVERSION SHEETS FOR TABLES 32
-------
LIST OF FIGURES
No. Page No.
7.1 Ames Test 10
7.2 In Vitro Cytotoxicity Assays 11
7.3 Rodent Toxicity Assay 12
LIST OF TABLES
No. Page
1. Definition of Effectiveness Categories 3
2. Primary Data Transititon Form 5
3. Health Effects Critical Data Summary Form: Mutagenicity .... 6
4. Health Effects Critical Data Summary Form: Toxicity 7
5. Ecological Effects Critical Data Summary Form 8
6. Bioassay Summary 9
7. Bioassay Summary Table 16
8. Health Effects Critical Data Summary Form: Mutagenicity .... 1?
B. Ecological Effects Critical Data Summary Form 18
10. Health Effects Critical Data Summary Form Toxicity 19
11. Bioassay Summary Table 20
12. Health Effects Critical Data Summary Form: Mutagenicity .... 21
13. Health Effects Critical Data Summary Form: Toxicity 22
14. Ecological Effects Critical Data Form 23
15. Bioassay Summary Table 24
16. Health Effects Critical Data Summary Form: Mutagenicity .... 25
17. Health Effects Critical Data Summary Form: Toxicity 26
18. Ecological Effects Critical Data Summary Form 27
IV
-------
I. INTRODUCTION
This report presents a system to convert results from Level 1 health
effects, bioassays, and selected Level 1 ecological bioassays into a
uniform format. This format is structured so that data can be converted
from original study reports into nondetectable, low, moderate, and high
designations. The evaluation of raw data and development of the final
toxicity definitions require a set oflTtalbe^and data transition sheets
that provide a uniform method of data stJIMarization.
The approach is based on the following rationale:
Biological activity measured by lethality has sufficient,
common phenomena to produce meaningful comparisons. The
Ames Salmonella assay measures gene revertants rather
than toxicity, but its results can be grouped in a similar
fashion.
Each assay, regardless of the type of response measured,
has a maximum applicable dose above which the test data
are virtually impossible to interpret because of nonspe-
cific responses of the test organisms.
A structure is needed for data assessment that can cate-
gorize toxicity data from diverse sources into a series
of similar comparative categories. Without this, the
comparison of these diverse assays would be difficult,
and subject to variable interpretations.
The categories low, moderate, and high are sufficiently
broad to encompass normal variability and species differ-
ences, yet are narrow enough to provide meaningful cate-
gories for decision making.
Level 1 testing is designed to rank sampling sites for
further studies and is not intended for making human risk
estimates.
Environmental assessment requires evaluation of data derived from chemistry,
engineering, and biology. This data formatting program was developed to
permit biological data to easily fit into the evaluation process without
requiring the user to be an expert in numerous biological disciplines.
The data forms suggested here will be revised as Level 1 procedures are
changed. Consult the most recent revision of "IERL-RTP Procedures Manual:
Level 1 Biological Tests for Environmental Assessment" for current forms.
-------
II. DEFINITION OF HIGH, MEDIUM. LOW, AND NONDETECTABLE TOXICITY
Definition of the above classes of toxicity would be as follows:
Nondetectable--no significant response as determined by a preestablished
set of criteria. Toxicants could be present in a sample below the level
of detectability (MDC) for the assay.
Low--a significant response or an LD50, LC50, or EC50 value ranging from
the maximum dose applicable to the assay system (defined as MAD and estab-
lished in advance) to one-tenth of that value. For example, if it is
established that 10 g/kg will define the upper limit of sample application
to rats in the acute rodent toxicity test, then any sample which has an
LD50 value of 10 g/kg down to a concentration of 1 g/kg will be defined
as having low toxicity.
Medium—a significant response or an LD50, LC50, or EC50 value ranging
from a concentraton less than the lower limit of the "low" toxicity (1/10
of the MAD) to 1/100 of the MAD. Using the same illustration, an LD50
(EC50) obtained at a concentration ranging from 0.1 g/kg to 1.0 g/kg in
the rodent toxicity assay will be considered as moderate.
High—a significant response or an LD50 (EC50) value less than 1/100 of
the MAD for the particular assay. Thus, any sample with an LD50 less
than 0.1 g/kg in the rodent assay will be considered to have high toxicity.
A summary of the toxicity levels and their associated definitions are
shown in Table 1.
The degree of sample concentration will be factored into the calculation
procedure and will reduce the level of toxicity accordingly.
Figures 1 to 3 illustrate the relationship of critical data (e.g. LD50)
to high, moderate, low and nondectable designations.
The MAD values are taken from current protocols used in conducting these
studies. The values proposed for MAD in Level 1 screening were established
from:
pilot study data developed from Level 1 testing,
scientific publications dealing with uniform testing methods
(Science, 203:563, 1979), and
standard operating procedures defined by testing labora-
tories experienced in conducting Level 1 bioassays.
-------
TABLE 1
DEFINITION OF EFFECTIVENESS CATEGORIES
RANGE OF CONCENTRATION OR DOSAGE
ASSAY
Ames Test
RAM/WI-38
and
CHO TOXICITY
RUDbNT
TOXICITY
Aquatic Tests
Algae
F1sh
Inverte-
brate
ACTIVITY MEASURED
Mutagenesis*
Lethality EC50
Lethality LD50
Growth Inhibi-
tion EC50
Lethality LC50
Lethality LC50
UNITS
mg/plate
pi/plate
mg/ml
ul/ml
Ml/ml
gm/kg
gm/ liter
%
gm/ liter
%
gm/llter
%
MAD
5
50
1
600
20 **
10
1
100
1
100
1
100
HIGH
<0.05
<0.5
<0.01
<6.0
<0.1
<0.01
<20
<0.01
<20
<0.01
<20
MODERATE
0.05-0.5
0.5-5.0
0.01-0.1
6.0-60
0.1 -1
0.01-0.1
20-75
0.01-0.1
20-75
0.01-0.1
20-75
LOW
0.5-5
5-50
0.1-1
60-600
1 -10
0.1-1
75-100
0.1-1
75-100
0.1-1
75-100
NOT DETECTABLE
NO at >5
NO at >50
ND at >1
ND at >600
ND at >20
NO at >10
ND at >1
ND at >100
ND at >1
ND at >100
ND at >1
ND at >100
ATt>b~revTatTons:"
MAD = Maximum Applicable Dose (Technical Limitations)
L050 * Calculated Dosage Expected to Kill 50* of Population
LC 50 = Calculated Concentration Expected to Kill 50* of Population
EC50 = Calculated Concentration Expected to Produce Effect in 50% of Population
NO = Not Detectable
•Negative response at 5 mg/plate or at level of toxlcity is jgiven as ND.
Positive resoonse requires calculation of minimum effective concentration (MEC) to produce a positive
mutagenic response. H, M and L designations are made from MEC values of positive agents.
"Volumes used for solvent exchange samples (This maximum keeps DMSO below level of toxicity).
-------
III. DATA TRANSITION FORMS
In order to ensure uniform translation of raw data to definitions of
toxicity, three intermediate forms were developed.
The Primary Data Transition Form (Table 2) records the basic information
from a test by sample. The type of test, is respective MAD, and the
calculated active dose are recorded on this table. Since the Ames
Salmonella assay is a special case, the LC50 value is not directly
applicable. The Ames test is evaluated as either positive (+) or nega-
tive (-). If it is (-), the level of activity is not as significant a
the fact that the sample was not mutagenic. If the sample was (+),
however, it is possible to assess the level of activity by defining the
lowest concentration of the test sample at which mutagenicity could be
detected (activity is defined by the preestablished criteria suggested
in Appendix A).
The primary form is also used to record testing methods which concen-
trate a sample after receipt, such as solvent exchange. The concentra-
tion factor is the ratio of the total volume sampled to the final sample
volume. There is also a place to record commments regarding the assay
that provide backup documentation or explanations. In several tests
(e.g., RAM), several parameters indicative of cell lethality are measured.
Each of these should be recorded in the primary data transition form
(Table 2) and the most sensitive parameter used to determine "Level of
toxicity" for the Bioassay Summary (Table 6). Without some method of
ranking each parameter, this is considered an adequate approach to
uniform data interpretation.
The Primary Data Transition Forms are often used when results are sub-
mitted by more than one biological contractor. It is designed to pro-
vide a uniform base for assimilating data for transition to subsequent
Level 1 formats.
Health and Ecological Effects Critical Data Summary Forms, (Tables 3, 4,
and 5) combine the data from Level 1 tests conducted on samples from a
single site. Tables 3 and 4 summarize reponses from health effects
tests and Table 5 summarizes the response from aquatic tests.
Tables 2 to 5 contain the responses which are finally summarized for all
Level 1 tests in Table 6. It is a summary of the various tests using
only ND, L, M, and H response designations. Table 6 allows rapid ranking
of the streams evaluated by the variety of tests used in Level 1.
-------
Table 2
PRIMARY DATA TRANSITION FORM
Assay: Sample:
MAD: Date Processors Name:
Results:
LD50 [EC50] Value8 [or] Ames Response
MECb
Approximate Concentration Factor
Level of ToxicltyC
Comments:
aFor the RAM assay the EC50 for the most responsive parameter will be listed.
bMEC = Minimum effective concentration [if response is positive].
cSee Table 1 for definition of toxicity levels.
dMay include;
Salmonella strains responding to a mutagenic substance.
Deviations from standard protocols.
Toxic signs in rodent assay.
Procedural problems.
-------
TABLE 3
HEALTH EFFECTS CRITICAL DATA
SUMMARY FORM: MUTAGENICITY
Contract No.
Site Sampled
Technical Directive or Project No.
Sample
Identification
Salmonella Plate Assay
Response3
LRPCU
I
.given as + or - based on criteria in Section VII.
LRPC = lowest recorded positive concentration
-------
TABLE 4
HEALTH EFFECTS CRITICAL DATA SUMMARY FORM:
TOXICITY
Contract ID.
_Technical Directive or Project ID.
Site 10.
UIU
Sample Toxicity
Identification LC50
RAM Cell Toxicity HI-38 Cell Toxicity Rodent Toxicitv
Viability3 ATPD Protein0 Viabilit
3 ATPb Protein0 LD50 Toxic Sinn«;d
i
\
Viability Index (see EPA 600/7-77-043)
J>TP (see EPA 600/7-77-043 for derivation)
^Protein (see EPA 600/7-77-043 for derivation)
Toxic sicjns are Identified in a numbered list 1n EPA 600/7-77-043.
Only the number is reported here
-------
TABLE 5
ECOLOGICAL EFFECTS CRITICAL DATA SUMMARY IORI1
00
I No
Sample
[dent if Scat ion
technical Directive or Project No
Fresh Water Toxic itv Tests3
Site sampled
Marine Tox icily Tests
fish Invertebrate ALGAL Fish Invertebrate AIGAL
LC5U LCSO EC50 iCbO LCM EC50
-- - -- — - -
ilei ivaLions of all test values are explained in the text of the report of the Level 1 manual.
-------
TABLE 6
BIOASSAY SUMMARY
Technical Directive or Project No..
Contract No.
Fresh Water
Marina
Sample Identification / *>
I I
i i
NO = No Detectable Toxicity
L = Low Toxicity
M = Moderate Toxicity
H = High Toxicity
-------
TEST RESULTS
[BASED ON EVALUATION CRITERIA]
I NEGATIVE |
SAMPLE I TOXICITY
DESIGNATED AS NO
[NOT DETECTABLE]
posmvEj
MEC_ ASSIGNED TOXICITY
RANGE VALUE
>0-r-
0.05 mg/plate—
0.5 mg/plate—
5.0 mg/plate _L
HIGH
MODERATE
LOW
The Maximum Applicable Dose [MAD] is set at 5 mg of substance per plate.
Figure 7.1 AMES TEST
10
-------
LD50 or EC50
RANGE
> 0
0.01 mg/ml _|_
0.1 mg/ml
1.0 mg/ml
>1.0 mg/ml
ASSIGNED TOXICITY
VALUE
HIGH
MODERATE
LOW
NOT DETECTABLE
1. CHO calls use viability index [EC50].
2. For RAM or WI-38 assays use the EC50 value from the
most sensitive parameter [viability, ATP, Protein].
Figure 7.2 JN VITRO CYTOTOXICITY ASSAYS
n
-------
LD50 [g/kg]
RANGE
> 0
0.1 g/kg ___
1.0 g/kg __[_
10 g/kg
>10 g/kg
ASSIGNED TOXICITY
VALUE
HIGH
MODERATE
UOW
NOT DETECTABLE
The Maximum Applicable Dose [MAO] Is set at 10 grams of substance
per kilogram of rat body weight.
Figure 7.3 RODENT TOXICITY ASSAY
-------
IV. ABBREVIATIONS USED
MDC = Minimum Detectable Concentration
MAD = Maximum Applied Dose
LD50 = Lethal Dose to 50% of the Test Organisms
LC50 = Lethal Concentration for 50% of the Test Organisms
EC50 = Effective Concentration for 50% of the Test Organisms
MEC = Minimum Effective Concentration
LRPC = Lowest Recorded Positive Concentration
13
-------
V. APPLICATION OF THE MAXIMUM APPLIED DOSE CONCEPT FOR ASSIGNMENT OF
TOXIC LEVELS ~'—
By examination of already existing pilot study reports (supplied by
EPA), test results were formatted using the methods described in this
report. Numerous parameters could be considered in this data transfer,
but we proposed a simple route relying primarily on LD50, LC50, EC50, or
MEC. The data analyses given in reports submitted to EPA by various
testing laboratories were amenable to this approach, and data conversion
into toxic levels was accomplished readily.
Summaries of results from the application of this approach are shown in
Tables 7, 11, and 15.
14
-------
Legend to Tables 7, 11, and 15
These tables are a summary of EPA submitted data for Level 1 Pilot Studv
Bioassays.
The data were obtained from bioassays of various water effluents (Table
7), fluidized bed combustion samples (Table 11), and coal gasification
procedures (Table 15).
Toxicity is defined as:
H = High
M = Moderate
L = Low
ND = Nondetectable
Tables 8, 9, 10, and 12, 13, 14, and 16, 17, 18 are intermediate summary
tables. Reported values are given as concentration based on Table 1.
15
-------
TABLE 7
BIOASSAY SUMMARY
Technical Directive or Project No..
Water Effluent
Contract No..
Pilot Study Results
Fresh Water
Marine
Sample Identification
Notes
A2
B2
C2
D2
E2
F2
62
H?
J2
K2
L2
M2
N2
P2
R2
S2
T2
U2
V2
W2
X2
h
22
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
M
L
L
H
ND
L
ND
L
ND
M
ND
L
M
N.D
ND
ND
ND
ND
NP
ND
ND
ND
ND
ND
ND
ND
N.D
ND
ND
NP
f*D
NP
ND
NP
ND
ND
H
ND
M
ND
NP
M
ND
ND
M
ND
M
ND
H
ND
M
ND
M
M
ND
ND
ND
H
ND
M
H
L
M
NP
ND
M
M
H
ND
H
ND
H
LI
H
ND
ND
M
I
L
M
ND
H
.
ND
L
ND
ND
M
ND
H
M
L
ND
NP
ND
ND
L
ND
ND
H
1
M
ND
M
ND
ND
ND
L
M
ND
M
ND
M
ND
1
M
ND
L
ND
ND
ND
ND
M
ND
ND
M
ND
1
|
1
ND
L
M
M
M
L
H
H
H
ND
L
ND
|
M|
NP.
1
1
1
1
i
i
1
I
i
j
:
1
i
CMC >20Q»I
NO * No Detectable Toxiclty
L » Low Toxlcity
M • Modnrate Toxiclty
H * High Toxlcity
LB1-0468 3'79
Data transition sheets for all results
not shown in Section VIII.
16
-------
TABLE 8
HEALTH EFFECTS CRITICAL DATA
SUMMARY FORM: MUTAGENICITY
Contract No.
Pilot
Sita Sampled
WE
Technical Directive or Project No.
Sample
Identification
Salmonella Plate Assay
Response
LRPC°
A2
82
C2
02
E2
F2
GZ
H2
J2
K2
L2
M2
N2
P2
R2
S2
T2
112
V2
W2
xz
Y2
Z2
-
-
-
-
-
-
-
-
-
-
_
-
-
.
-
-
.
.
-
-
-
-
.
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
sgiven as * or - based on criteria fn Appendix A
LUPC = lowest recorded positive concentration (NA for negative data)
17
-------
Contract No.
00
TABLE 9
ECOLOGICAL EFFECTS CRITICAL DATA SUMMARY FORM
Pilot
Technical Directive or Project No.
NA Site sampled WE
Fresh Mater Toxicltv Tests3 Marine Toxicity Tests8
Sample
Identification
Fish Invertebrate ALGAL Fish
LC50 LC50 EC50 LC50
Invertebrate ALGAL
LC50 EC50
A2
B2
C2
D2
E2
F2
G2
H2
J2
K2
L2
M2
N?
P2
R2
S2
T2
U2
V2
W2
X2
Y2
Z2
19%
>100%
46.5*
>100%
>100%
64.7%
> 1003!
>100%
25.5%
>100%
48.8%
>100%
16.5%
>100%
46.5%
>100%
36%
55.2%
>100%
>100%
>100%
9%
>100%
41.0%
7.8%
81.7%
62.4%
>100%
>inn*
28.0%
60%
10%
>100%
8%
>100%
12.1%
9.4%
6.3%
>100%
>100%
42.6%
76%
30%
>100%
2%
96%
>100%
>inn*
42.0%
>100%
2%
43%
93%
>100%
>100%
>100%
>100%
94%
>100%
>100%
18%
60%
>100%
70%
>100%
>100%
iinn*
>100%
40%
>100%
70%
>100%
56%
>100%
25%
>100%
>100%
>100%
>100%
•>1100%
25%
»100%
>100%
25%
>100%
>100%
90%
25%
70%
59%
77*
2%
2%
9%
>100%
70%
>100%
94%
50%
>100%
aThe derivations of all test values are explained in the test of the report of the Level I manual,
-------
TABLE 10
HEALTH EFFECTS CRITICAL DATA SUMMARY FORM: TOXICITY
Contract ID. Pilot
CHO
Sample Toxic ity
Identification LC50
Technical Directive or Project ID. NA
RAM Cell Toxicity HI-38 Cell Toxicity
Viability3 ATP° Protein0 Viabilit a ATPb PrntHnc
Site 10. up
Rodent Toxicity
L050 Toxic Sinns*3
A2
B2
C2
02
F?
F2
G2
H2
J2
K2
L2
M2
N2
P2
R2
S2
T2
U2
V2
W2
X2
Y2
Z2
60 ,,1
>200 pi
75 Pi
37 ul
400 P!
13.3 Pl
.600 ul
33.5 ul
357 ,,1
175 Pl
600 yl
>600 ul
>600 pi
>600 pi
300 pi
>600 yl
200 P!
>600 yl
-
>600 P!
>600 pi
IQq/kq
10q/kq
lOg/kg
10g/ kg
10q/kq
lOq/kq
lOg/kg
lOg/kg
10q/kg
lOq/kg
10q/kq
lOg/kg
lOg/kg
lOq/kq
10q/kq
lOq/kq
10g/ kg
lOq/kg
lOg/kg
lOq/kq
lOg/kq
lOg/kg
lOq/kg
None
Nnnp
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
^Viability Index (see EPA 600/7-77-043)
bATP (see EPA 600/7-77-043 for derivation)
^Protein (see EPA 600/7-77-043 for derivation)
°Toxic signs are identified in a numbered list
in EPA 600/7-77-043. Only the number is reported here
-------
Technical Directive or Project No
Contract M Moderate Toxicity
H - High Toxicity
L3:-04«8 3/79
20
-------
TABLE 12
HEALTH EFFECTS CRITICAL DATA
SUMMARY FORM: MUTAGENICITY
Contract No. Pilot Site Sampled
Technical Directive or Project No. NA
Sample
Identification
Salmonella Plate Assay
Response8 LRPC
lu Cyclone
3u dye lone
Dolomite
Flyash
Soent Bed Dolomite
Dolomite Leachate
foal Leachate
+
+
-
-
-
-
-
2 mq/plate
2 mg/plate
NA
NA
NA
NA
NA
1
jjgiven as * or - based on criteria In Appendix A
LRPC * lowest recorded positive concentration (Negative data = NA)
21
-------
TABLE 13
ro
ro
HEALTH EFFECTS CRITICAL DATA SUMMARY FORM: TOXICITV
Contract ID. Pilot
_Techn1cal Directive or Project ID. NA
Site 10. FBC
CIK)
Sample Toxicity
RAM Cell Toxicity WI-38 Cell Toxicitv Rodent Toxicitv
Identification LC50 Viability3 ATPD Protein0 Viability
3u Cyclone
Fly Ash
Dolomite
Fly Ash Leachate
Slurry
Spent Bed Leachat
1 Cyclone
Scent Bed Dolomit
Dolomite Leachate
>1000uQ
>1000uq
>1000uq
> 600p1
> 600pl
> 600^1
>1000uq
>1000ug
> eooui
>1000)^q
>1000uq
>1000ug
> 600^1
> 146pq_
> 600^1
> 596ua
>1000uy
> 600ul
>1000uq
>1000uq
>1000uq
> 600ul
> eoowi
> 600gl
>1000uq
MOOOug
\> eooui
3 ATPb Protein0 1050 Toxic Sinnsd
»
>10n/ka
>10g/kg
>10ml/kg
>10ml/kg
>10ml/kg
>10g/kg
>10ml/kq
NONF
NONE
NONE
NONE
NONE
NONE
NONE
Viability Index (see EPA 600/7-77-013)
ATP (see EPA 600/7-77-043 for derivation)
^Protein (see EPA 600/7-77-043 for derivation)
Toxic signs are identified in a numbered list In EPA 600/7-77-043. Only the number is reported here
-------
CO
TABLE 14
ECOLOGICAL EFFECTS CRITICAL DATA SlftWRY FORM
Contract Ho. Pilot
Sample
Identification
Technical Directive or Project No. NA
Site sampled
Fresh Water Toxtclty Tests*
FTsli Invertebrate AlGAT
LC50 LC50 EC50
Marine Toxic100%
>100X
92%
67.9%
>100%
40.9%
>100%
>100%
70.9?
13.6%
> 10055
45%
>100%
>100%
19%
9%
>100%
>10OT
>100S
>100S
11.5%
70. 5%
>100%
74.8%
72.5%
66%
18%
>100%
56%
>100%
>100%
62%
aThe derivations of all test values are explained In the text of the report of the Level 1 manual.
-------
TABLE 15
BIOASSAY SUMMARY
Technical Dlractiv* or Pro/act No
Contract M« Pilot Studv Data
Coal Gasification (CG)
Frash Wattr
Marint
Simpl* Identification
NO m No D«t«etabla Toxicity
L » Low Toxicity
M m Mod*rat* Toxicffy
H . High Toxieity
*Data from all samples
designated Separator
Liquor combined.
Notes
Separator Liquor*
590-3 (XAD-2)
655-3 (XAD-2)
655-5/6 (XAD-2)
590-5/6 (XAD-2)
Coal
Coal Dust
Cyclone Dust
Separator Tar
Ash
ND
L
NP
ND
ND
M
ND
M
H
M
H
H
ND
ND
ND
L
L
M
H
H
M
I
i
ND
ND
ND
ND
H
H
-
!
H
H
1
H
H
I
i
1
I
I i
1 '
! 1
i ;
i \
] :
24
-------
TABLE 16
HEALTH EFFECTS CRITICAL DATA
SUMMARY FORM: MUTAGENICITV
Contract No. Pilot Site Sampled CG
Tectinical Directive or Project No. NA
Sample
Identification
Salmonella Plata Assay
Response
LRPC"
590
655
Coal
Ash
Cyclone
Separator Tar
Separator Liquor
+
-
-
-
-
-
-
1 mq/plate
NA
NA
NA
NA
0.01 mq/plate
NA
1
r
i
i
i
i
i
?given as * or - based on criteria in Appendix A
LSPC = lowest recorded positive concentration (Negative data = NA)
25
-------
ro
en
TABLE 17
HEALTH EFFECTS CRITICAL DATA SUMMARY FORM: TOXIC1TY
Contract ID. Pilot
JTechnlcal Directive or Project ID.
NA
Site ID. CG
Sample
Identification
CIIO
Toxiclty RAM Cell Toxicity
LC50 Viability3 ATPD Proteinc Viahilit
Vll-38 Cell Toxiclty
5 90- 3
655-3
Coal
655-5/6
590-5/6
Coal Dust
Separator Tar
-Cyclone Dust
Seoarator Liouor
Asfi
-
21.2 wl
>1000 yl
< 6.0 yl
< 6.0 wl
>1000 yg
350 ug
^1000 vg
12 yl
>300 yg
5.3 vl
-
>1000 pi
'
-
>1000 vg
-
>1000 yg
-
>300 ug
-
.
>1000ul
-
<6.0 \>~\
>1000 vg
-
>looo ug
-
>300 yg
<6.0 pi
<6.0 v]
7.4 yl
-
-
-
-
>10q/kg
>10g/kg
>10g/kg
>10g/kg
NONE
NONE
NONE
NONE
ATP
Protein0
Rodent Toxicity
LD50 Toxic
.Viability Index (see EPA 600/7-77-043)
"ATP (see EPA 600/7-77-043 for derivation)
^Protein (see FPA 600/7-77-043 for derivation)
Toxic si(jns are identified in a numbered list in EPA 600/7-77-043. Only the number Is reported here
-------
TABLE 18
ECOLOGICAL EFFECTS CRITICAL DATA SUMMARY FORM
Contract No. Pilot
Technical Directive or Project No.
NA
Site sampled CG
Fresh Water Toxlclty Tests* Marine Toxlclty Tests8
Sample
Identification
Separator Liquor
Fish Invertebrate ALGAL Fish Invertebrate ALGAL
LC50 LC50 EC50 LC50 LC50 EC50
<1.0%
<1.0%
<1.0S
<}.0%
-------
VI. DATA ORIGIN
SOURCE CONTRACT # DATE
Northrop Services, Inc. 68-02-2566 1978
EG&G Bionomics - 1977
SRI 68-01-2458 1977
SRI - 1978
Battelle 68-02-2138 1977
Litton Bionetics, Inc. - 1977
Monsanto Res. Corp. 68-02-1874 1978
28
-------
VII. AMES TEST DATA INTERPRETATION CRITERIA
A. Evaluation Criteria
Plate test data consist of direct revertant colony counts obtained from
a set of selective agar plates seeded with populations of mutant cells
suspended in a semisolid overlay (Ames et al. , 1975). Because the test
chemical and the cells are incubated in the overlay for 2 days, and a
few cell divisions occur during the incubation period, the test is semi-
quantitative in nature. Although these features of the assay reduce the
quantitation of results, they provide certain advantages not contained
in a quantitative suspension test:
The small number of cell divisions permits potential mutagens
to act on replicating DMA, which is often more sensitive than
nonreplicating DMA.
The combined incubation of the compound and the cells in the
overlay permits constant exposure of the indicator cells for
2 days.
1. Surviving Populations
Plate test procedures do not permit exact quantitation of the number of
cells surviving chemical treatment. At low concentrations of the test
chemical, the surviving population on the treatment plate is essentially
the same as that on the negative control plate. At high concentrations,
the surviving population is usually reduced by some fraction. Our protocol
normally employs several doses ranging over 2 or 3 log concentrations,
the highest of these doses being selected to show slight toxicity as
determined by subjective criteria.
2. Dose-Response Phenomena
The demonstration of dose-related increases in mutant counts is an important
criterion in establishing mutagenicity. A factor that might modify dose-
response results for a mutagen would be the selection of doses that are
too low (usually mutagenicity and toxicity are related). If the highest
dose if far lower than a toxic concentration, no increases may be observed
over the dose range selected. Conversely, if the lowest dose employed
is highly cytotoxic, the test chemical may kill any mutants that are
induced, and the compound will not appear to be mutagenic.
29
-------
3. Control Tests
Positive and negative control assays are conducted with each experiment
and consist of direct-acting mutagens for nonactivation assays and mutagens
that require metabolic biotransformation in activation assay. Negative
controls consist of the test compound solvent in the overlay agar together
with the other essential components. The negative control plate for each
strain gives a reference point to which the test data are compared. The
positive control assay is conducted to demonstrate that the test systems
are functional with known mutagens.
4. Evaluation Criteria for Ames Assay
Because the procedures used to evaluate the mutagenicity of the test
chemical are semiquantitative, the criteria used to determine positive
effects are inherently subjective and are based primarily on a historical
data base. Most data sets are evaluated using the following criteria:
a. Strains TA-1535, TA-1537, and TA-1538
If the solvent control value is within the normal range, a
chemical that produces a positive dose response over three
concentrations with the lowest increase equal to twice the
solvent control value is considered to be mutagenic.
b. Strains TA-98 and TA-100
If the solvent control value is within the normal range, a
chemical that produces a positive dose response over three
concentrations with the highest increase equal to twice the
solvent control value for TA-100 and two to three times the
solvent control value for strain TA-98 is considered to be
mutagenic. For these strains, the dose-response increase
should start at approximately the solvent control value.
c. Pattern
Because TA-1535 and TA-100 are both derived from the same parental
strain (G-46) and because TA-1538 and TA-98 are both derived
from the same parental strain (D3052), there is a built-in
redundancy in the microbial assay. In general, the two strains
of a set respond to the same mutagen and such a pattern is sought.
It is also anticipated that if a given strain, e.g., TA-1537,
responds to a mutagen in nonactivation tests, it will generally
do so in activation tests. (The converse of this relationship
is not expected). While similar response patterns are not
required for all mutagens, they can be used to enhance the
reliability of an evaluation decision.
30
-------
d. Reprociuc ibi 1 i ty
If a chemical produces a response in a single test that cannot
be reproduced in one or more additional runs, the initial posi-
tive data lose significance.
The preceding criteria are not absolute, and other extenuating
factors may enter into a final evaluation decision. However,
these criteria are applied to the majority of situations and
are presented to aid those individuals not familiar with this
procedure. As the data base is increased, the criteral for
evaluation can be more firmly established.
5. Relationship Between Mutagenicity and Carcinogenicity
It must be emphasized that the Ames Salmonella/Microsome Plate Test is
not a definitive test for chemical carcinogens. It is recognized, however,
that correlative and functional relationships have been demonstrated between
these two end points. The results of comparative tests on 300 chemicals
by McCann et al. (1975) show an extremely good correlation between results
of microbial mutagenesis tests and j_n vivo rodent carcinogenesis assays.
B. References
Ames, B.N., McCann, J. and Yamasaki, E. (1975). Methods for detecting
carcinogens and mutagens with the Salmonella/mammalian-microsome mutage-
nicity test. Mutation Res. 31, 347-364.
McCann, J. , Choi, E. , Yamasaki, E. and Ames, B.N. (1975). Detection of
carcinogens as mutagens in the Salmonella/microsome test: Assay of 300
chemicals. Proc. Nat. Acad. Sci. 72, 5135-5139.
31
-------
VIII. DATA CONVERSION SHEETS FOR TABLES 7. 11, AND 15
Abbreviations of Sample Source
FBC = Fluidized Bed Combustion
CG = Coal Gasification
WE = Water Effluent Samples
Organisms Employed in Aquatic Tests
Freshwater
Fish Fathead Minnow (Pimephates promelas)
Algae Selenastrum capricornutum
Invertebrate Daphnia Magna
Marine
Fish Sheepshead Minnow (Cyprinodon variegatus)
Algae Skeletonema costatum
Invertebrate Grass Shrimp (Palaemonetes pugio)
32
-------
PRIMARV
DATA TRANSITION FORM FBC
ASSAY-. Grass shrimp SAMPLE: Coal leachate
MAO: IQOt
RESULTS:
IDSO |EC60| VALUE* 96 hour LC50 - 66t |W| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR n_
LEVEL OF TOXfCiTY* Moderate
COMMENTS:
24 hr toxlclty - 75J effluent
48 hr toxlclty * 72.51 effluent. Dfss. 02 - 42t of saturation
*Fw •>• RAM »*•* »» ECM tor ttw mo»l inpomln pMMiMtw MlH tw Mil*.
b«IEC - Minimum •llocllra concmlnlkHi | II i«pon» to poillhri)
"SM T*M« 1 loi itollnlllon ol la«ldly Iwtli.
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: Gr>" Shr<"P SAMPLE:
MAO:
RESULTS:
LOW |ECSO| tfALUE* >-CSO ' 72-5* E at 96 hr- K»l *"E« RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITY
e Moderate
COMMENTS:
24 and 48 hr. LC&O's = 751 E
*F« MM RAM »i«r HM ECM lor KM moll rnponih* p««intaf MM to Retell.
bMEC • Minimum tltecU** concmlrillaii III mpom* to patllhr*)
*8M TdM* 1 lor (Mtntllofi ol loiMly Imll
33
-------
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY- Grass Shrimp
MAD:
SAMPLE:
Dolomite Leachate
RESULTS:
LD60 IECSOI KAIIIE* LC50 » 74.8« I at 96 hr.
LEVEL OF TOXICIir
. |m| AMES RESPONSE
„b
MFC
APPROXIMATE
CONCENTRATION FACTOR 0
COMMENTS:
24 and 48 hr.
LCSO's • 100J E.
E * Effluent
Vo. MM HAM ••••? m« ECtO lot Hw mo.l rnponthw pwmwlw
•M b* Itetad.
bMEC - Minimum ellmllv* eone«nlr«llon (II mpomt to poflllra).
"$•• I«bl« 1 lor dcllnlllon ol lo«kllj (•«•!«.
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY: Grass Shrimp
MAD: 100* E
SAMPLE:
N 9661 (Slurry)
RESULTS:
LDSO IECSO) VALUE* IC50 • 181 at 96 hr.
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
High
. fw| AMES RESPONSE
MEC
COMMENTS:
48 hr. LC50 ' 34.5t Effluent.
Vot Mi* RAM *•••? MM ECM lar «w mod
bMEC - Minimum •ll«ctln conc*nl>*llan | II
°SM T«bl» 1 l« dtlmlllon ol laikllf tonli.
34
-------
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY: Grass shrimp
MAD. 100» Effluent
SAMPLE- Spentbed leachate
RESULTS:
LD60 |EC80| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
Hot Toxic >100»
ND
|«| AMES RESPONSE
_b
COMMENTS: Not detectable
Dlss. Oj ' 551 of saturation at all test levels.
*F
-------
PRIMARY
DATA TRANSITION FORM
Fish toxlclty Dolomite leachate
"**5>^ T . — — . .—. — — _..---— -T "AMPlt ; -_.-.._
FBC
MAD:
RESULTS:
1060 fecsoi VALUE* *>t toxic at 1001 (M| AMES BESPONSE
M6Cb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
NO
COMMENTS: Not detected
No toxidty after 96 hr. exposure. Diss. 02 after 96 hrs. ranged from 26X of saturation
in lot of effluent to 481 of saturation in the control
and lOOt effluent.
*For Ih* RAM itiiy Hi* ECSO lor Ihl moll f«pontl«« pwwmlM wlH b* Uiltd.
bMEC - Minimum tlltcllx conctnlnllon III rttponu to poilll«|.
CS.« T«bl« 1 l« d*nnlllon ol l
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: Fish toxlcity _ SAMPLE: N 9 661 (Slurry)
100X _
RESULTS:
LDSO |Ecso| VALUE* LC5° * 70.5»E «t 19 hr. _ )o,, AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
c Moderate
COMMENTS:
Not toxic at 24 and 46 hr exposures.
*For *• RAM MUf Nw ECM foe OM matt rMpomhr* pwMMtof mm b» N(l*d
bMEC • Minimum •ll*clhr* conewikMkm | II mporn* h po»W»»|.
etM T«M« 1 tor ftellnlllon ol loitdly
36
-------
PRIMARY
DATA TRANSITION FORM FBC
Marine '
ASSAY- F RAM >i»* llw ECSO In Ih* moil impentht pMiimlw will b* M«l«d.
bMEC - Minimum •M.cllv* concmliclton III mponM I* po»lll»«|
CS»« Tibl« 1 lex dcllnlllon of lonlclly Imlt.
PRIMARY
DATA TRANSITION FORM FBC
Marine
»cc.v F»AT . „ oAMrLc:
MAD:
RESULTS:
1060 |EC50| VALUE* Not toxic »t 96 hrs. (>100») |«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL Of TOXtClty' NB
COMMENTS:
01ss 02 • 481 of saturation In all tests.
"Fw MM RAM M»r Nw ECSO for MM ne*l >»lpon*tv» p*r*m*t*r »M b*
bMEC - Minimum dtaeHM canCMikalhM IH rmpeiM* H pe«Wn|.
"SM T*M« 1 lot
-------
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY:
MAD:
Marine
Fish toxiclty
1001
SAMPLE:
Spent bed leachate
RESULTS:
LOSO |ECSO| VALUE* Not tOXJC at 100*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
|of) AMES RESPONSE
MEC
NO
COMMENTS: Not detected
No mortality after 96 hr. exposure. Olss. 0.- >_ 571 of saturation
In all test levels.
Vet Nw RAM Mil? Itw ECM loc MM moil iMpontht pwamclw *m b* M(l«d.
bMEC - Minimum •llmlln conc*nb*llon III rnpom* I* po«lll«|
eSM Tlbto 1 (Of iMInlllofi ol lenlclly l«**l*.
38
-------
PRIMARY
cor
DATA TRANSI1ION FORM
Freshwater
ASSAY: A1ga1/DAPHNlA/F1sh SAMPLE: Dolomite leachate
MAO:
RESULTS:
LDSO |ECtO| VALUE* > 100»/> 100*/ > TOO* __ |m| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0 / 0 / 0
LEVEL OF TOXICITV NO / ND / ND
COMMENTS: Not detectable
*Fof MM MAM ••••» llw ECSO Iw ttw mod i(lpoMl» pwmwtot mill b* tUlwl.
bMEC . Minimum •IIMII» connntf lllon III mponu li potlll»»|
"SM T*M« 1 foe (Mlnlflon of loildly !•>•!•.
PRIMARY
DATA TRANSITION FORM
Freshwater
ASSAY- Algal/DAPHNlft/Flsh SAMPLE' sPC"t Bed Leachate
MAD: 100*
RESULTS:
LOW |ECSO| VALUE* ' 45t/40.9/25 |M| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR Q / Q / Q
LEVEL OF TOXICITY Moderate/Mnrii.r»t»/Mnrf»f«fi.
COMMENTS:
*Fot MM RAM Mur Hw ECSO la MM mo*l rMpamhr* pw«n*lM »4H IM
bMEC - MMmum •Itoellra concmmllm IH rnpomi to pocHIra).
*SM Tibta 1 Im (talMUoii al lo>lclly \n*t.
39
-------
PRIMARY
DATA TRANSITION FORM
FBC
Freshwater
ASSAY- Algal/DAPHNlA/Fish
MAO:
1001
SAMPLE:
Flyash Leachate
RESULTS:
LDSO |EC50| VALUE* > 10W * 100*/ > 100»
|of| AMES RESPONSE
Jt
NEC
APPROXIMATE
CONCENTRATION FACTOR 0 / 0 / 0
LEVEL OF TOXICITY
NO / ND / ND
COMMENTS: Not detectable
*FM lh« RAM »»y lh» ECSO lor Hi* moil mpontln p««ml« will b« ll»l«d.
bMEC - Minimum •ll«cll» concmlnlkm | II »ipom« It poilll«*|.
CSM T«bl« 1 lot dclMllon ol loilclly I*M|I.
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY:
MAO:
Freshwater
Algal/DAPHNWFIsh
1001
SAMPLE:
Coal Leachate
RESULTS:
LOW |ECM| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICirV*
/ 70.9 / 921
0/0/0
High / Moderate / Low
|oc| AMES RESPONSE
MEC
COMMENTS:
*Fef Hi* RAM MMT Nw ECW to ttw me*l i«H>
bMEC . Minimum *ltocH» eoncMkithm | H
••M Tibta 1 tor iMnlUwi ol leiMly tmt*.
IM wM b. Rtlctf.
!• potlMra)
40
-------
PRIMARY
DATA TRANSITION FORM FBC
Freshwater
Algal/OAPHNIA/F1sh MUp,r. Scrubber Slurry
ASSAY: 3 ' .....-.>. .j.. SAMPLE
MAD
RESULTS:
1060 lECSOl VALUE 9* / 31.6J / 67.9» _ |OT| AMES RESPONSE
MEG"
APPROXIMATE
CONCENTRATION FACTOR 0/0 f Q
LEVEL OF TOXKMTV" Hinh / MivW«t» / Moderate
COMMENTS:
•foi HM RAM •!••» M» ECM lo> lh» moil »«|Min«ln PMMIMIW wW b* Nilid.
bMEC • MMimim •Itaclln* concmlcclhm III i«ponl« I* po«lll>*|
BS«* T«W» 1 lof dcllnlllon ol lo>ldly •*••!•
41
-------
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY: Marine Alga
MAO: 100%
SAMPLE: Spent bed leachate
RESULTS:
LOW |ECM| VALUE* Hot Toxic >100»
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
COMMENTS:
Optical density Increased by effluent.
. |or| AMES RESPONSE
MEC
*F<* »• RAM ••§•» MM ECM loi lh« moil i*tponihrt pwimctM wltt b* Hiltd.
bMEC - Minimum •Itecllra concwilfitlon I II ra«pon» to pmlllra).
lac iMmHIofi of lo>lc(ly
PRIMARY
DATA TRANSITION FORM
FBC
ASSAY- Harlne Alga
MAO:
SAMPLE:
Coal leachate
RESULTS:
Unaltered effluent
LDM |EC50| VALUE Not tOXlC
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
. K»l AMES RESPONSE
MEC"
COMMENTS: Not detectable
Filtered effluent gave ECBO's at 98. 39 and 38» effluent for
4, 8 and 12-day exposures.
*F« MM RAM MM* DM ECM »
-------
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: _^rjn,eJ^ SAMPLE: N 9661
MAD: 100%
RESULTS:
LDSO IECSOI VALUE* EC50 62% of effluent at 4 days |o.| AMES RESPONSE
MECh
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY Moderate
COMMENTS:
The ECSO's reported for unaltered effluent were 62% for 4 days and 66% for
12 days exposure. Filtered effluent was more active that unaltered effluent
with ECSO's of 22 and 15t for 4 and 12 days.
"For lh« RAM ••>•? I'M EC60 lor III* motl i»pon»U« pMtmelw will t» Ililx)
''MEC = Minimum •Itacllv* conc«ntr«l(on | II »«ponn li poslllra).
CS*« Tibia 1 lor ddlnlllon ol lo«lclly Intli.
PRIMARY
DATA TRANSITION FORM FBC
ASSAY Marine alga SAMPLE- Dolomite leachate
MAD: 100*
RESULTS:
LDSO |EC5o| VALUE* EC50 ^ 561 effluent |<,,| AMES RESPONSE
b
MEC
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY Moderate
COMMENTS:
Filtered effluent gave ECSO's aS 191 and 241 for 8 and 12 days respectively.
*Foi MM RAM ••••( MM EC6O lor III* motl itiponilra pw*m*lM will t» H»l«d.
bMEC • Minimum •ll»cll«« conCMiliillon III icipontt li po«llln|.
°S«» T«bl* 1 lot itoMnllton ol lonklly
43
-------
PRIMARY
DATA TRANSITION FORM FBC
ASSAY- Marine Alga SAMPLE- Fly»sh leachate
MAO: IQCHt effluent
RESULTS:
LDSO (ECMH VALUE* Mot tOXlC >l(KMi |«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV*
COMMENTS: Not det«Ct«bl«
*Fat MM RAM ««Mr Mw ECM let MM moil IMP«I*|*» pwiiMlM wM b*
bMEC - Minimum *ll«eHn eoncwikcltan III iMpom* to po*Hln|.
44
-------
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: Kodent toxiclty 8AMpLE; Fly Ash
MAD: !£.
RESULTS:
LD50 |ECSO| VALUE* > 10 g/kg |of| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY NnnHptpi-taM f
COMMENTS:
No toxic sign reported.
'FM Mi* RAM M»r Ih* EC60 l« lit* moil rnpontln pMimilci will b« Htlcd.
bMEC - Minimum •ll»cll«» concmliillon | II mpom* !• po«lll»«|
°Sn T«bU 1 lor dallnlllon ol lankily I«»U.
PRIMARY
DATA TRANSITION FORM FBC
Rodent toxicity Dolomite
ASSAY: SAMPLE:
MAD: 10 "/kg
RESULTS:
LD60 |EC50| VALUE* 10 9/kg _ (o|| AME8 nESpONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITY Nondetectable
COMMENTS:
Ho toxic sign reported.
*Fof Hi* RAM »»y lh« ECH In Hi* moil mponih* pwnntlM will b« Hil*d.
bMEC - Minimum •Mcellra conc«nh«lk>n | II i.tponi* It po«llln|.
°SM T.W. 1 Im ddlnlllon ol loildlr l***l<.
45
-------
PRIMARY
DATA TRANSITION FORM
„ Rodent Toxlclty «.«..« Spent Bed Dolomite
SMP
ASSAY: ' SAMPLE:
MAD: 10 9/kg
RESULIS:
LDtO |ECBU| VALUE* > 10 9/kg |or| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXtCIIY* Mnnd»ti»i-tahli»
COMMENTS:
No toxic signs reported.
*F« Hi* RAM »t«y III* EC60 lot UM moil i«pon«l>« pMcnMlM win b« MlUd.
bMEC - Minimum •ll*ell«» conemilmllon III nsponra !• po«lll««|.
CS*» Ttbl* 1 lof d.ltnlllon of loildty tonli.
PRIMARY
DATA TRANSITION FORM FBC
ASSAy. Rodent Toxlclty _ SAMPLE- Sp*"1 B*1* Leachate
MAO. 10 g/fcg or 10 ml/kg
RESULTS:
LDSO |ECW| VALUE* > 10 l»1/kq _ |«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
COMMENTS:
No toxic signs reported.
*Fgr Nw RAM »••* UM ECM tar Irw IMMI rvipoMlra pnunttt, «MI b* *tl«d.
bMEC . Mhilinum •HwMn coneMtfeMKm | H ratpom* It podllw).
aSM TiM» 1 lot dcUntllo* eH loitdly tmtt.
46
-------
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: Rodent Toxtclty SAMPLE- Fly Ash slurrV Sample
MAD: 10 g/kg or 10 ml/kg
RESULTS:
1050 |EC50| VALUE* > 10 ml/kg )of| AMES
MECb_
APPROXIMATE
CONCENTRATION FACTOR n
LEVEL OF TOXICITY Nnnri»t»rtfhl P
COMMENTS:
No toxic signs reported.
*Fix III* RAM «•«•» lh« EC60 lot lit* moil itipontlx pwimilcr will b« Mi led
**MEC - Minimum •Ilicllv* conccnliillon III ratponi* It poclll««|
CSM T«t)l« 1 loc d«llnlllon ol lo«Icily l»tl>
PRIMARY
DATA TRANSITION FORM FBC
Rodent toxfclty Fly Ash Leacnate
ASSAY.- SAMPIE: __j;
MAD: 1Q9A9 00 ml/kg)
RESULTS.
1060 |ECSO| VALUE* > 10 ml/Kg |«| AMES BESPONSE
MECb
APPROXIMATE
CONCENTHATION FACTOR 0
LEVEL OF Toxiciif". Nondetectable
COMMENTS:
No toxic sign reported.
*Fof »• RAM MMV UM ECM loc Hw moil icipontln pwim»i« will Iw lltlwl.
bMEC - Mkilmum •ll«cll«* eoncmballon IH ntpon** It potlil»|.
CS»» Tilil* 1 loi dVMIIon ol lonklly l«»el.
47
-------
PRIMARY
DATA TRANSITION FORM
FBC
RAM
ASSAY: _
MAD: 600
•AMPLE:
Slurry (Fly Ash)
RESULTS:
LD60 |EC6O| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
146 ul/ml (ATP fg/cell)
|m| AMES RESPONSE
MFC
Inn
COMMENTS:
Range of ECSOs for all parameters • 146 - > 1000 M!.
*F« MM RAM MM* llw EC60 la Hw moil ittponilv* pwMMtof en* b* ««wd.
bMEC - Minimum •Iwelhi* conc.nh.llon | II r«pom* U po»IH«.|
"SM Table 1 kw itallnllloa ol loildly !•»•!•.
PRIMARY
DATA TRANSITION FORM
FBC
RAM
ASSAY:
MAO: 1000 ug/ml
•AMPLE: 17 - 3 u Cyclone
RESULTS:
LD60 |ECM| VALUE
APPROXIMATE
CONCENTRATION FACTOR
' >1000 U6/HL
LEVEL OF TOXICITV
Mnndgteetablg
. |oc| AMES RESPONSE
MEC
COMMENTS: All parameters
"For MM RAM nuy «M ECM lor MM HM»| f
bMEC - Minimum •Itecflrc ooneMilrMkm |N
•SM Tibw 1 to.
-------
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: _™! _ SAMPLE: K ~ *" f11ter Cyclone
MAD: 1000
RESULTS:
inso IECSOI VALUE* 596 uo/ml (ATP fa/cell) _ |W| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITrC Low
COMMENTS: ND In other parameters
*F« 111* RAM ituy Ih* ECM Iw llw mod l«pon»l»* fwiMmUr will b« Ililwt.
bMEC - Minimum •llacll» concniliillon III mponi* U poiltlv*)
CS«« T*bl* 1 lof ddlnlllon ol lonlclly !•«•!•.
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: «" SAMPLE: °°}t»,ite
MAO: 600 ul or 1000 tig/ml
RESULTS:
LOW IECSOI VALUE* > 1000 fS/ml |or| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR n
LEVEL OF TOXICITY Nondetectable
COMMENTS: All parameters
*For MM RAM •••«» lh« ECM Iw Un nwil i*tpon«l«* p*iwn*l« wIN b« Mllcd
- Minimum •Iliellv* conc*nlr«llon | II i»pon>* li po«lll»«|
T«M* 1 hH d*lhi«lon ol loildlj tonli.
49
-------
PRIMARY
DATA TRANSITION FORM FBC
ASSAY: RAM SAMPLE: Spent bed Dolomite
MAO: 600 pi or 1000 ug/ml
RESULTS:
LOSO |EC60| VALUE* >1000 uq/ml . |of| AMES RESPONSE
_b
NEC
APPROXIMATE
CONCENTRATION FACTOR Q_
LEVEL OF TOXICITY* NnnHotar
COMMENTS: All parameters
*Fix ih» RAM Miiy MM EC60 lot the moil iMpontlv* parameter wIN b* NtlMt.
bMEC - Minimum •llmllw conccnlnllon III »*pom* It potlllnj.
CS«» Tibl. 1 loi definition ol lonlclly l»v.l«.
PRIMARY
DATA TRANSITION FORM FBC
^ . SABLES:
ASSAY: _ . «*"PI*f tnan^t"^ iMriufa
MAO: 600 .lor 1000 .g/ml ^ash ^"^
RESULTS:
LOSO |EC60| VALUE* > 1000 uQ Or ul/nl |or| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICIIY
COMMENTS. All parameters'
*Fw ll» RAM ••«•! Hi* ECM Idf ttw moil iMponilM pMmwtai MM b« M.Ud
bMEC - MkikninH •ItoeMM conc*nl»lkm III r«pon» !•
BSM T«M* 1 lac d.llnlMon al loildlf to««li.
50
-------
PRIMARY
DATA TRANSITION FORM
FBC
Salmonella
ASSAY:
MAD: 5 ing/plate
SAMPLE:
Spent Bed DolonUe
RESULTS:
LDM |EC60| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXKITY
. |or| AMES RESPONSE Negative
MEC
Nnnrietectahlo
COMMENTS:
Maximum concentration tested Mas 5 mg/plate.
*F« lh« RAM »»y In* ECM loi lh« mo*l i»»pon*l>» pMtRMlw will b* H«Ud.
bMEC • Minimum •IfocHw concmb iHon | II nipont* U posllU*).
°SM T»W« 1 lot definition ol lonklly
PRIMARY
DATA TRANSITION FORM
FBC
Salmonella
ASSAY:
MAD: 5 nig/plate
SAMPLE:
Ash
RESULTS:
LOW |ECM| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY"
0
Nondetectable
. lot) AMES RESPONSE Negative
Jb
MEC
COMMENTS:
Maximum concentration used was S ing/plate.
*Fw Hw RAM ••••» MM ECM lot ttw miMl i««pandv* pw«ml« wMI Iw Hil*d
bMEC - Minimum •U»cU» ooncmUillan | II ictponti li poilll««|.
••M T«W« 1 taf dvllnllMM el lo.Wly
51
-------
PRIMARY
OAIA TRANSITION FORM fBC
ASSAY: Sahnonel_la____ Dolailte
MAO: 5 mg/plate
RESULTS:
LOSO (EC60| VALUE* . («1 AMES RESPONSE Negative
MEC"
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY Nondetectable
COMMENTS:
Maximum concentration tested MAS 5 mg/plate.
*Foi Hi* RAM H»y UM ECM loi Hw moil raipontb* pwimclw wIM be Hiltd.
bMEC - Minimum •Itocllm concmlicllon | II mponM li po*IUv*|.
°Sm T«bU 1 lor ditlnlllon ol loilclly l««*ll.
PRIMARY
FBC
DATA TRANSITION FORM
ASSAr. Salmonella SAMPIE: 3u Cyclone Coarse Participate
MAO: 5 nig/plate
RESULTS:
LDM leCMI VALUE* |«| AMES RESPONSE
2 mo/plate
APPROXIMATE „
CONCENTRATION FACTOR
LEVEL OF TOXICITY" lo>*
COMMENTS:
Active In strains TA-1538 and TA-98. Sample was more active
without S9 than with S9 at equivalent concentrations.
•FOT KM RAM ••••» HM ECM tor lh« matt Mtpondra PWMMIM wIN IM Ril«d
bMEC • Minimum •ftoclln cone«nh*llon | II (••pom* If potlttra).
*SM Tibta 1 tor MlnlHon ol |o>MI( ImU.
52
-------
PRIMARY
cor
DATA TRANSITION FOI1M
.... Salmonella •••••• r. ^ Cyclone Fine Paniculate
ASSAY' _ i QrtWrLS, , _ - , —
MAD: 5 ing/plate
RESUUS:
L050 |EC50| VALUE* |«| AMES RESPONSE Positive
MECb 2 mg/plate
APPROXIMATE
CONCENTRATION f ACTOR n
LEVEL OF TOXICITY
COMMENTS:
The sample was active In strains TA-1538 and TA-98.
More activity was obtained In nonactivation tests (-S9)
than In activation tests (+S9) at equivalent concentrations.
'fa MM RAM ••••> lh« ECSO lot MM mo»l i»«pomln pMWtMtot win b* M«l*d
bMEC - Minimum •tl«cll>« concwiliiUiMi IH »>penM !• patllln).
eSM Tefal* • l« d.lhiMloo ol tonlctlr Imcli.
53
-------
PRIMARY
DATA TRANSITION FORM
CG
ASSAY: Marine A'fl*1
MAO: - 100 nx,/} nr
SAMPLE: Holston Separator Liquor
RESULTS:
1050 |EC60| VALUE* 0.69% (A day?), 0,531 (B
O.Alt (12 days)
APPROXIMATE
CONCENTRATION FACTOR Filtered
LEVEL OF TOXICITY*
|M| AMES RESPONSE
NEC-
COMMENTS:
V« MM RAM Mtn KM ECM lor MM moil i«ipon»lr« pw
-------
PRIMARY
DATA TRANSITION FORM CG
ASSAY- .FH Algal Assays SAMPLE- Radian Separator Liquor
IIAD: 100 ing/ 11 tcr or 10OT
RESULTS:
IDSO IECGO) VALUE* 0.1 - U |w| AMES RESPONSE
MEC"
APPROXIMATE
CONCENTRATION FACTOR
IEVEI OF TOXICITY High
COMMENTS:
*Foi HM RAM M»y KM ECM lot MM moil inponiln IIMMIMIW win to IUI*d.
bMEC - Minimum •llMllM conccntiallon 11| nipenit U po«MI»|
••M T»Wt 1 UK itollnlllon ol lonklly towlt.
PRIMARY
DATA TRANSITION FORM CG
FW Algal Assays
ASSAY: SAMPLE: HnUtnn «;»pji^tpr Liquor
MAD: 100 mg/llter or 1QM
RESULTS:
LDM |EC«o| VALUE* 1.9J (4 days). o.57» (B daysk HN| AMES RESPONSE
0.531 (12 diys) „
MEC
APPROXIMATE
CONCENTRATION FACTOR Unf1U»rAri
c High
LEVEL OF TOXICITY
COMMENTS:
"Fof MM RAM ••••» Hw ECM l« «M Mod iMpmtlM pwMMtaf «rtH to Nttad.
kMEC • Minimum •Itacll** eoncwtbiHOfl | H inpoM* to po*lll««|.
•••• T*M« 1 bf iMInHiM ol
55
-------
PRIMARY
DATA TRANSITION FORM CG
ASSAY: "'-» .AMPLE: 655 - 5/6
HAD: 600 gl or 1000
RESULTS:
.-V
LDSO |EC50| VALUE* 1.3 x Id" » (Viability index) |«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITYq (High)
COMMENTS:
Cannot Interpret concentration relative to established MAD.
*Fof MM RAM MM* MM ECM lor lh« moil (Hponiln pwMMln "MM b* Nitod.
bMEC - Minimum •lt*Ell>« eoncmlfilkm III mpon» It pralllv*)
aS«) T«M« 1 lot
-------
PRIMARY
DATA TRANSITION FORM CG
Separator Liquor
UT 1A MR 011
ASSAY: "' SAMPIE: "" ' UIJ
MAO: 600 pi/ml or 1000 n9/«i1
RESULTS:
tow (ECtoi VALUE* 7.4 u1/»1 (viability Index) . |«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
level OF TOXICITY"
COMMENTS:
NAD was 60 gl/nl for this sample.
*F« *• RAM nuv MM ECW taf «M moil rMpomhr* pvwMlw wW b* ltal*d.
"MEC - Minimum •ItoellM cencmkilloii IN inpont* to pmHhn).
C»M T*M» 1 l« dclMHoH ol leildly
57
-------
PRIMARY
DATA TRANSITION FORM
CG
ASSAY: FW Fish Test SAMPLE: HoUton Separator Liquor
MAO: 100 mq/1 or 100%
RESULTS
ISO |EC60| VALUE _
0.161
LD50 |EC60| VALUE 0.231 l?« hrs}. 0.201 (48 hrsK |l»d.
bMEC - Minimum •ll»cll>* conc»nli»lton III mponu I* potllln).
CSM T»bU 1 Iw dcllnlllon ol lo.lcll» Itxlt
PRIMARY
DATA TRANSITION FORM CG
ASSAY Freshwater Fish Test MMPIE: Radian Separator Liquor
MAO: 100 mg/11ter or loot
RESULTS:
LOW |ECM| VALUE* 0.02* (96 hrs) |c«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICHY
COMMENTS:
High.
*Fo» tfn RAM MM? lh« ECU tot lh« moil ictponiM pMimtlw will b* H*l«d.
bMEC - Minimum •H*cll» conctnliltlon III »>ponM !• pa*lll>»|
°8«» T«bl* I lor dillnlllon of loilelly I
58
-------
PRIMARY
DATA TRANSITION FORM CG
ASSAY- Daphnlt Assay BAMM.E: Rtdian Separator Liquor
MAD: 100 ing/liter or 10M
RESULTS:
IDSO |EC«H VALUE* O.Slt (24 hrp - p.1U (48 hrs) |ec| AMES RESPONSE
EC"
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXtCIU* High
COMMENTS:
*Faf MM HAM MM? «M ECH to MM MMl tiipoml.t pwwiMtar mm b* Httad.
•>M6C - Minimum «lMcU
-------
PRIMARY
DATA TRANSITION FORM
ASSAy. Salmonella SAMPLE: HB-019 (HAAP cyclone dust)
MAD: S ing/plate
RESULTS:
LOSO |EC50| VALUE |ot| AMES RESPONSE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITT" Nondetectable
COMMENTS:
Maximum concentration tested was 5 nig/plate.
Vor IN* RAM H»f lh» ECSO loc III* moil lllpontNf pmcmtlm will b* Nllwl.
bMEC - Minimum tllmcttn cone*nl»llon IM r*>pon» l» po»lll««|
CS«« T»bl« 1 lot dollnlllon ol loilclly l*»lt.
PRIMAflV
DATA TRANSITION FORM CG
ASSAV. Salmonella SAMPLE: HB - 018 (HAAP ash)
MAD: 5 nig/plate
RESULTS:
LD&O |Ecw| VALUE" |w| AMES RESPONSE Negative
«iecb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV Nondetectable
COMMENTS:
Maximum concentrate tested was 5 ing/plate.
V« Bw RAM ••»y HI* ECSO lor llw moil rwpontb* pwcnwtor wlH IM Ntlcd.
bMEC - Minimum •llcclln conctiilnllon III mpoiw* It ptMllln)
°S«« T»W. 1 lot dallnlllon ol lankily !•«)•.
60
-------
PRIMARY
DATA TRANSITION FORM
CG
A8SM: Salmonella SAMPLE. Coal
MAO: S ag/plate
RESULTS:
LOW |ECM| VALUE l«l AMSS RESPONSE Negative
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY" Nondetectable
COMMENTS:
Naxlnun concentration tested Mas 5 tig/plate.
*F«r *• RAM Mur Mw ECM l« MM HHM! iMpomliM puMMlM oM b* Hitad.
^MEC » Minimum •llKll»* MfWMkiUoii IH i»«poi«»t It po«lll««|
*SM Tibto 1 to dMMIlim e( toifctl, *»•(•.
PRIMARY
DATA TRANSITION FORM CG
A$8A¥. Salmonella SAMPLE- HB - 020 (HAAP Coal)
MAD: 5 BO./pi ate
•JESUITS:
LDM (ECtoi VALUE*. |ar| AMES RESPONSE Negative
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY* Nondetectable
COMMENTS:
Did not «eet preestabllshed criteria for Mtagenlclty. However, the report
Indicated this sample was weakly mUgentc for strains TA-98 and TA-100. A
concentration of 10 "a/plate produced less than 2-fold Increases over the
v. HH^Mec.U°i^U%^hniirtJQUJ^Pi,JUAL *»
*MEC - MMmum ^toellv* oonemtf allon IH iMpom* to po«Hln|.
*SM Tabta 1 IM dclMttan ol loildly towl*.
61
-------
PRIMARY
DATA TRANSITION FORM CG
ASSAY: Salmonella SAMPLE: 59°
MAD: 5 ing/plate
RESULTS:
1060 |ECM| VALUE* |o,| AMES RESPONSE Positive
1 »>9/p1ate
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY*
COMMENTS:
This sample was nutagenlc for TA-1537. TA-1S38. TA-98 and TA-100 only under
conditions of activation (+S9).
*Fot MM nAM M»y MM ECU lor HM mml mponclll pwwiwtof WIN b* IliUd.
bMEC - Minimum (Hccllm concmlrelloit (II ntpooM to p
-------
PRIMARY
DATA TRANSITtOM FORM CG
ASSAY: __jalmone11a SAMPLE: H8-017 (HAAP Separator liouorl
MAD: 5 ing/plate
RESULTS
LDSO |EC60| VALUE* (f»| AMCS RESPONSE Negative
MEC*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV*
COMMENTS:
The max) mini concentration tested was 5 mg/plate.
*Fo» Ih* RAM Mtcy MM ECSO lof MM motl iMpontln pMimdtT «riM |M Nttad.
•»MEC - Minimum •lUctlw eone«nlf*Non IH i*tpo«»* It po»MI»|
*S« T*M* 1 lot dcllnlllon of leildly !•*•!•.
MIIMARV
DATA TRANSITION FORM CG
AMAV. Salinonelld SAMPLE: H-032 (HAAP Separator tar)
MAD: _S mg/pUte
RESULTS:
LOW |ECM| VALUE* l"l AMES RESPONSE Positive
M6Cb 0.01 ng/plite '
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITV* High
COMMENTS:
The sample was Mutagenfc for strains TA-1537, TA-1538.
TA-98 and TA-100.
Aclttvlty was observed only with S9 activation.
*Fof MM RAM *•••« MM ECM lot «M mott mpontfra pMMwwi «M b* Itatod.
•>MEC - Minimum •!»«»*• cntcwnlnMoii IM ntfonn It pMll*m).
C5t» T«M« 1 to ddlnlllofi ol lenkllr MMM.
63
-------
PRIMARY
DATA TRANSITION FORM
CG
ASSAY: Rodent Toxlclty
MAO: 10 q/kq
SAMPLE:
Ash
RESULTS.
ID60 |ECSO| VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
> Ifl a/to
Ho ndetec table
. |0(| AMES RESPONSE
MEC
COMMENTS:
No toxic sign reported.
*F« MM RAM ••••? Ih* ECW lot MM nml rMpomta* pM«mwf mm tw Nfl*d
bMEC . Minimum •IfKllv* concwllf >tlon I II <»ponM to po*IU 10 q/kq
Mondetectable
I AMES RESPONSE
MECb
COMMENTS:
No toxic sign reported.
'Fat MM RAM ••»•* ttM ECM lot «M
bUEC - Minimum •IlKllw
CSM T*M* 1 lot
-------
PRIMARY
DATA TRANSITION FORM
CG
ASSAY: Rrutfnt Tnnirity SAMPLE:
MAD: 10 q/kq
RESULTS:
LDSO |EC50| VALUE* > in n/tn
LEVEL OF TOXICITY
. |tt| AMES RESPONSE
MEC
APPROXIMATE
CONCENTRATION FACTOR 0
Hondetectable.
COMMENTS:
No toxic signs reported.
*For tt» RAM Mtly llw EC60 lot llw moil icipontlv* PWMIMIM w
bMEC - Minimum •Itocllv* concmlnllon III mponu li po«llln|.
°SM T*bl* 1 lot ddlnlllon ol loilclly I««U.
I b« Nstod.
PRIMARY
DATA TRANSITION FORM
CG
ASSAY:
MAD:
Rodent Toxlclty
SAMPLE- HAAP Cyclone Dust
RESULTS:
LDSO |ECSO| VALUE* > 10 9/fc9
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
(Of) AMES RESPONSE
MEC
Nond«tart«h1a
COMMENTS:
No toxic sign reported.
'Fat •>•
bMEC - Minimum •llMlhM eonc*nl»llon I H
aS«* T»W« 1 lor otllnltlon ol lonkHly !«•!•.
65
-------
PRIMARV
DATA TRANSITION FORM
CG
Rodent Toxlclty
SAMPLE- H**P SeP""itor Liquor
MAD: 10 gAg
RESULTS:
1050 |EC60| VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY*
> 10 9/kg
0
Nondetectable
|M| AMES RESPONSE
MEC-
COMMENTS:
No toxic signs reported.
*Fof Hn RAM »•••» HM EC*0 In lh« moil mpoml**
bMEC - Minimum •HwUM eoncwrit«Uo« IH CMponc* to po«MI
-------
PRIMARY
DATA TRANSITION FORM CG
«** SAMPLE: 590'3
MAO:
600 pi/ml
RESULTS.
LOW |EC*o| VALUE* 5.3 yl/al (ATP fg/cell) |m| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXtCITt* High
COMMENTS:
Several parameters showed toxlclty 1n this range
(5.3 - 13.5 for all parameters).
*F« «M RAM ••••» MM ECM tor MM mod f«ipon«lm pwuiMlw win (M *>l«d.
bMEC • Minimum dUcllv* eonc«ilr*Non III »»po«n* It
*S«« T«M* 1 Iw dclbilllon ol lo»kll» ta«tl«.
PRIMARY
DATA TRANSITION FORM CG
ASSAY: MM SAMPLE: 655'3
MAO: firm |.l/«i1
RESULTS:
LOSS |ECM| VALUE 21T? ul/al (tfiah lnri»») |Of| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITY" Moderate
COMMENTS:
Range of EC50 for all paraneters • 21.2 - 101.7.
•FW •» RAM MUf •>• ICSS tat «M mo»t inpatMhr* PWMIMIM «M b* Itotatf.
kMEC - Mtnlmom cltoeM** cone«»lrtlloii IN imponn It pMHhw|.
*SM TAta 1 tec dclMUoo •« toildly
67
-------
PRIMARY
DATA TRANSITION FORM
ASSAY: RAM SAMPLE: Coal
MAO: finn ,,i nr inno ,,B/mi
RESULTS:
LDSO |EC50| VALUE* > 1000 up/ml |W| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR Q_
LEVEL OF TOXICITV Nonde tec table
COMMENTS: All parameters
*Fot Hw RAM «MV HM ECM lof MM im»l ittpondr* pM
-------
PRIMARY
DATA TRANSITION FORM
CG
RAH
ASSAV:
MAD: 600 pi or 1000
SAMPLE: 590 - 5/6
RESULTS:
LDSO |EC60| VALUE* > 4 x IQ'H (Cell count and protein 1 |w| AMES RESPONSE
MEC
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
(High)
COMMENTS:
Cannot Interpret data relative to established MAD.
*f>MEC - Minimum ttlKtlw eoncMitnlkm IH i**pon» I* pqtllln)
BSM T«bl« 1 lof dcllnlllon ol loiklly Ivntt.
MMMARY
DATA TRANSITION FORM
CG
ASSAV:
RAM
1000
•AMPLE:
Coal dust
RESULTS:
LOM |ECSO| value* > 1000
APPROXIMATE
CONCENTRATION FACTOR
16VEL OF TOXICITT
. HM| AMES RESPONSE
MEG
COMMENTS: A11 parameters not detectable toxlclty
F« MM RAM ••••» •«• ECM to* Ow metl inponsln P«WM|W wM b*
l»dhi« eofw«ibillo« I H inpom* to po«HH«|.
•M T«M* 1 «« iMInmwi •! loiiMly tend.
69
-------
PRIMARY
DATA TRANSITION FOAM CG
ASSAY: _^! SAMPLE: HB ' °"
HAAP ASH
SAMPLE: _
HAO: 600 nl or 1000 yg/m}
RESULTS:
LDSO IECSOI VALUE* > 300 pg/»i1 (all parameters) |wj AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR
e
LEVEL OF TOXICITV* Low
COMMENTS:
No data > 300 pg/nl; thus cannot Interpret.
*Fof MM RAM MMV *<• ECM lot «M mo«l mpotwlv* pwMMIw «riN b* (toted.
bMEC - Minimum ««l»c«M conc»ol«»llo« | II iMpon» ll po*Hlra|.
lot d*Hnltkm ol le>ldl|r !
PfllMARY
DATA TRANSITION FORM
CG
ASSAY: »» .AMPtt: « " «"«
COAL
SAMPLE: ** '
MAD: 600 ill or 1000 n9/»l
RESULTS:
LOM IECMH VALUE* > 1000 uo/al tal^ |«l AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITV" Mondettctable
COMMENTS:
'For MM RAM M»» KM ECM to MM nod m»»ml.« p«Mwlw »M k*
bMEC . Mtabmim «ltoe««* concMtkMkm IM m»aim to
*S*« T*bl« 1 tat (MlnHhM •! toiMIr
70
-------
PRIMARY
DATA TRANSITION FORM
HAAP Cyclone Oust
ASSAY : _BftM _______ SAMPLE : _ H6 - OTS
MAD: 600 |.T nr 1000 |.Q/ml
RESULTS:
LOW |EC50| VALUE* - jJOOO ug/ml (all) _ __ |0|) AMES RESPONSE
MEC"
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY0
COMMENTS:
*F« lh« RAM ••••( Ih* ECM l« (In moil i*ipon«U* pwimtln will l» tltlwl.
bMEC - Minimum •llacllx concinliillon III mponi* !• potlllvt).
°S«» T*M< < lor definition of loitldly t«««l«
PRIMARY
DATA TRANSITION FORM C6
Separater Tar
ASSAY: _J*« SAMPLE: m ' **
MAD: 600 nl/1000 pg/ml
RESULTS:
LD50 IEC50) VALUE 350 uQ/inl (Viab. Into*} ,„,, AME8 BESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITY0 Low
COMMENTS:
*fa Hw RAM ••••( Ih* ECN lot UK moil iNponthm pmrnclw wIM IM Itatad
bMEC - Minimum •IUcllo« conemliillon IH i««pom« It potllln)
°SM T*M* 1 lot dolmlHon el lotldly
71
-------
PRIMARY
DATA TRANSITION FORM CG
Separator Liquor
ASSAY: "*" SAMPLE: "* " °13
MAD: 600 nl or 1000 yg/«ll
RESULTS:
LDM IECMI VALUE". »Z ul/«1 (Viability Index) |«| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXreiTYC Moderate
COMMENTS:
•for Hi* RAM mur *• ECM Iw ft* MMl iMpOMlM pwMMtof «*t b* Itotod.
bMEC - Minimum •ItoeM** co«c«nlnMM IN ratpoM* to pMMW»|.
"SM Tabl* 1 lo. (MMHM •! kuldlr l
PRIMARV
OATA TRANSITION FORM
ASSAY: MM _ SAMPLE: Cyclone
MAO:
RESULTS:
LDM (ECMH VALU6* > 1000 ufl/.l
RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITV* NO
COMMENTS: Noo detectabU toxlclty all parameters
Vo> *» RAM MMr ft* ECM for ft*
bMCC • MbikMMi •IIM>«M tiincmilnlHii |H
*SM 1M» 1 lot
-------
PRIMARV
DATA TRANSITION FORM CG
ASSAY- Grass shrimp test SAMPLE: HoIston separator liquor
MAO: 100 mg/Hter or IIXMt
RESIRTS:
1060 |ECM| VALUE* O.m (48 hfO. 0.25% (96 hrO k*| AMES RESPONSE
MECb
APPROXIMATE
CONCENTRATION FACTOR Q
IEVEI OF TOXICITY' High
COMMENTS:
•F« KM RAM »»r HM fCM Im Nw mod iMponrtM pwimMw wM b* H*l«l.
bMEC • Minimum tltoclhra nmrankiHon IN inponra to
BSM Tlbto 1 IM ttolMHan ol laiteUy
73
-------
PRIMARY
DATA TRANSITION FORM
WE
ASSAY:
RAH
MAO: 600 ul or 1000
SAMPLE:
R-2-15
RESULTS:
LDSO (EC601 VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
> 600
|M| AMES RESPONSE
NEC
COMMENTS: All parameters
*Fof MM RAM ••••» Nw ECM l« KM mod inpoMln powiMlw «IN b« Milcd.
bMEC - Minimum •Itoclhr* coneonkoltoii III iMponM to po«lll»|.
eS«« TiM« 1 tot iMInMoii ol loiW
PRIMARY
DATA TRANSITION FORM
WE
ASSAY:
RAM
SAMPLE:
H-2-15
MAD: 600 nl or 1000 H9/*1
RESULTS:
LOW |ECun VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
Lux
(Vt»b. lnde»)
. |ec| AMES RESPONSE
MEC
COMMENTS:
tCSO es Una ted fron raw data.
*F« HM RAM it»| Hw ECM tot MM m«ll iMpoMhr* p»c
"MEC - MInlimm •nKU«» ooncMlunM IN rMpomi to
C»M T*M* 1 lot il^MHon el
74
-------
PRIMARY
DATA TRANSITION FORM
ASSAY:
RAH
MAD. 600 ul or 1000
8AMPIE:
N-2-'S
RESULTS:
LD&o |EC60| VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEV6L OF TOXICITv
175 ul/ml (ATP)
Lov(
AME8 BESPOHSE
COMMENTS:
EC50 estimated from raw data showing ECSO >60 and <200.
*F« Hw RAM ••••» lh« ECM loc lh« moil (•sponil** puamilw will b* llil*d.
bMEC - Minimum •Itaclhra concmliillon I II ratpom* It po«lll»»|
°8M T*bl« 1 lo> dellnlllon ol iQnlclly lt»*lt.
PRIMARY
DATA TRANSITION FORM
ASSAY:
SAMPLE:
MAO: 600 \i\ or 1000 w9/m1
RESULTS:
LDM |ECSO| VALUE* ' 300 nVml (ATP)
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
Inu
|or| AMES RESPONSE
MEC
COMMENTS:
EC 50 Estimated from raw data.
*F« MM RAM ttuy MM ECU lor MM moil rnpmralra pdrnwlw wUI b* Mttod.
«>MEC - Minimum •lwell>« concmtiilton | II icspon** l> potlllx)
°SM T.bl« 1 l« d.llnlllon ol lo«ldl» )•»!•
75
-------
• PRIMARY
DATA TRANSITION FORM
ME
ASSAY: CHQ
MAD: 6QO .,1/ml
SAMPLE: B.p.lfi
RESULTS:
10(0 |ECEO| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
|llM | N
"••» Table 1 lot tMtaHwn el wildly Mwte.
76
-------
. PRIMARY
DATA TRANSITION FORM
ASSAY:
MAO:
CHO
600 Ml/ml
SAMPLE:
M-2-15
RESULTS:
LDSO |ECBO| VALUE* > 200
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
. |ot| AMES RESPONSE
MEC
COMMENTS:
200 ul/ml was highest concentration used. Thus could not determine
ED50 In context of MAD.
*Fof *• RAM *tuy lh« ECBO lot llw moil mpomlv* pwwmltf will IM M*l*d.
b|iec • Minimum •ll«ellw conembcllon III r»«poni» It poilllv*).
"&M TiM« 1 lot dcllnlllon ol lonldlr
PRIMARY
DATA TRANSITION FORM
ASSAY:
MAO: .
CHO
600 pi/ml
SAMPLE:
L-2-15
RESULTS:
LDSO IECMI VALUE"
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
- 60
. |W| AMES RESPONSE
MEC
COMMENTS:
EC50 estimated from raw data. Value slightly > 60 wl/ml.
•FMEC - Minimum »ltocll«* conccntaMton IM wtpenn It potltlv*).
CSM Table 1 loi (MlnlMon ol loihHIy !•«•!•.
77
-------
PRIMARY
DATA TRANSITION FORM WE
ASSAY: MdHne *'a*e SAMPLE: _L1
MAO: 10°*
RESULTS:
LDSO |ECSO| VALUE* 56 - 751 |ot| AMES RESPONSE
_b
NEC
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXKITY
e Moderate
COMMENTS:
IC50 - 70* 901 norUHty with 1001 effluent.
*Fof Hi* RAM ••••» llw ECM lof Hw mo»l i*tpoml«* |MrwwlM «M b« Mtod.
bMEC - Mtnlmum •ltaeM«« coocwlMlkM IM rnpOM* It pMlU«*|.
"SM T*M« 1 lot (MMlton ol U»(c«»
78
-------
PRIMARY
DATA TRANSITION FORM
WE
UI-38
ASSAY:
HAD: 600 pi/ml
SAMPLE: R'2'15
RESULTS:
IDSO |EC60| VALUE > 600 u 1 /ml
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
|w| AMES RESPONSE
NEC
COMMENTS: All parameters
•for MM RAM «»y U>« ECSO lor Ih* moil r*tpon«l» pwfn»t« wIM b« N>l*d.
bMEC - Minimum •ll«cll» conc»r>lr»lton III ntponi* It potlllv*)
°SM T*M« 1 In dcllnlllon ol lonlclly Uvtli.
PRIMARY
DATA TRANSITION FORM
WE
ASSAY:
UI-38
SAMPLE:
H-2-15
MAO: SOOul/ml
RESULTS:
IDSO |EC50| VALUE* > 600 uVlltl
lot) AMES RESPONSE
MEG
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
COMMENTS: All parameters
*Fot Ih* RAM MMV lt» ECM lot NM mo«l ittpOMhr*
bMEC - Minimum •ll«ellra eoncmbillM I II
CS«« T«bl* 1 IM itollnlllofi ol loildlr U»»U
PWWMIM will b« Hitad.
79
-------
PRIMARY
DATA TRANSITION FORM
WE
ASSAY:
MAO:
UI-38
SAMPLE:
N-2-15
RESULTS:
LDSO IECSOI I/AIUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL Of TOXICITY
* 200
Low
(ATP)
|m| AMES RESPONSE
MEC
COMMENTS:
EC50 estimated from raw data.
*For III* RAM «t*y MM ECM lac III* mail i**pon«l» p*r*m*l*r will b* H*l*d.
bMEC - Minimum •ll*cll«* concmlc*llon III iMpmn* I* poiltlo*)
CS*« T*M* 1 lac fMlnlilon al loiiclly l*«*li.
PRIMARY
DATA TRANSITION FORM
ASSAY:
WI-3B
MA0. 600 ul/ml
SAMPLE:
L-2-15
RESULTS:
LDSO |ECM| VALUE* ' *<*> vV*l (ATP)
LEVEL OF TOXICIIV
APPROXIMATE
CONCENTRATION FACTOR 0
|ac| AMES RESPONSE
MEC
COMMENTS:
EC50 estimated from raw data showing EC50 >200 pi and <600 til/ml.
*Foc Ni* RAM •«••» Hw ECM loc HM motl cwpondv* pwMntac wM b* Hitad.
''MEC - Minimum *ll*cll«* conc*nli«lkM I H c*«poni* ll po*llhr*|
CS*. T*M* 1 lot dcllnlllon ol loikll» l«*l>
80
-------
PRIMARY
DATA TRANSITION FORM
UE
Grass shrimp
ASSAY: .
MAO:
loot
SAMPLE:
L-2
RESUITS:
LOW |EC«0| VAIUE*
APPROXIMATE
CONCENTRATION FACTOR
IEVEI OF TOXICITV
>100S
AMES RESPONSE
MEC
COMMENTS:
Non toxic at 100X.
*Fo» Hi* RAM «•••» lh« ECM lot HM mail iMpantlra pwmitlw will b* NtlMl.
•"MEC > Mli.ln.uoi vIlMlh* eonemliKlton I II ratpami li poilH>*|
aSM T«M* 1 lot itolMHoii el lonkHly
PRIMARY
DATA TRANSITION FORM
UE
ASSAY: Grass shrimp
MAO: innr.
SAMPLE:
N-2
RESULTS:
LOCO IECMI VALUE"
10 - 32*
. H»| AMES RESPONSE
MEC
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXtCITr"
Moderate
COMMENTS:
LC50 about 251
•FO« ll» RAM .l»r MM ECiO lot Nw MO.I iMpoml«» P«MIM|W wM b«
bMEC - Minimum •II«H» concwiHclkm | H ntpenit It potHlra|.
°SM Tabto 1 l« d^Mllon ol lo>Mly tonli.
81
-------
PRIMARY
DATA TMNSniON FORM
HE
ASSAY: fit-act <:hrl«|p SAMPLE: S-2
MAD: ]nm
RESULTS:
IDM |ECW| VALUE* - >10°* |«| AMES RESPONSE
ECfc
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITT*
COMMENTS:
20% toxtclty «t 100S effluent
*F« Nw RAM MM* HM ECM taf *• MMI i
bMEC - HMmrn* «NM«M .iniiiiiliiRiii IN
PRIMARY
DATA TRANSITION FOUM HE
ASSAY: -•" ••"••*• SAMPLE. " ' ?
MAD:
RESUtTS:
IDM IECMI VALUE* - >10W (•! *MCS
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXKITV*
COMMENTS:
Nontoxic at IOCS.
*FM MM MAM MMW Ik9 CCM tav MM ••••I fv^BMMlM wMMStaf vM kft ••tasl
*SM T«M* I tai MMH«i •» ImlcWy •*•*•
82
-------
PRIMARY
DATA TRANSITION FORM
ASSAY: Grass shrimp
MAO: 100*
SAMPLE:
W-2
RESULTS:
LOM |ECW| VALUE*
18 - 321
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
Moderate
AMES RESPONSE
MEC
COMMENTS:
1C50 ' 25X effluent.
•fa MM DAM n»r MM EGM lot HM mwl mpwral** pK
''MCC " Minimum dtoeMt* •OMMilriUoii IN i*tftn»» H
•SM T«M« 1 toi (MWIkm •! huMlf torrit.
PRIMARY
DATA TRANSITION FORM
ASSAY: Crass shrjnp
MAO:
SAMPLE:
1-2
KESULTS:
LOW |ECM| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
J8L
. M AMES RESPONSE
J*
COMMENTS: Not detectable
ftontoxlc at 100X
*F«c «M RAM MM* «M CCSS M» MM
|M
•SM TAto 1 tar 4*IMN«i •> toitaHy fcwto.
83
-------
PRIMARV
OAIA TRANSITION FORM
WE
ASSAY: Grass shrimp SAMPLE: A-?
MAD: IQOt
RESULTS:
LOCO |EC«0| VAIUE* 32 '
APPROXIMATE
CONCENTnATION FACIOfl
LEVEL OF TOXICITY"
Moderate
. to) AMES RESPONSE
MEC
COMMENTS:
LCSO at about 251 efluent cone.
*F« MM RAM ••••» DM ECM to Hw meet
bMEC • Minimum rttecH** c*«c«it»Ho* IH n
•SM Tabto 1 to MtaHtoi ol to.ldly tntlt.
iMpondn pwwiMwr «M b* Nttod.
PRIMARY
DATA TRANSITION FORM
WE
ASSAY: Grass shrlnp
MAO: IQOi
SAMPLE: B-2
RESULTS:
IOM |ECM| VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXKITV
>100X
JUL.
AMCS RESPONSE
COMMENIS
Ho toxlctty at 1001.
Vof MM RAM MMI MM ECM KM *w
"MEC - Minimum dtocllw evncMrti
|«
84
-------
PRIMARY
DATA TRANSITION FORM
HE
ASSAY-. Grass shrimp
MAO: IQOji
SAMPLE:
C-2
RESULTS:
LDH |EC«0| VALUE
.100*
AMES RESPONSE
APPROXIMATE
CONCENinAltON FACTOR
LEVEL OF TOXICITV
COMMENTS:
IOOS ' only 561 mrUMty.
"FOI *M RAM «•••» Hw ECM Iw «M mot
bMEC - Mbtlmum *lM«n«* MmeMihrilon IN
•SM TiM* 1 to dclMlhm ol t»»lelt» Mwl*.
PRIMARY
DATA TRANSITION FORM
ASSAY: Grass shrimp
100?
SAMPLE:
RESULTS.
IDSO |ECW| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY
>100f
. H*l
RiSPONSE
MEC
COMMENTS:
No toxIcUy at 100J.
•fa, MM RAM M
••M T«M* 1 toi d*IMNiM ol
iM b*
85
-------
PRIMARY
DAI A TRANSITION FORM
ASSAY:
MAO:
Grass shrimp
1001
•AMPIE:
G-2
RESULTS:
1060 |ECM| VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICIIY
>100J
HP
HM| AMES RESPONSE
MEC
COMMENTS:
Only 201 mortality at 100S effluent.
*Fw Hw RAM MMy DM ECM to MM mmtt »»t»DmM pvwMtw
*MEC - Minimum «IM««m cww«ilr«Mo« IN mppmi k
*SM Tcbto t lot iMbiNtoii •! loiklly lm«l>
PRIMARY
DATA TRANSITION FORM
ASSAY: Grass shrlny
MAD: 1001
SAMPLE
. K-2
RESULTS:
LDM |ECM| VALUE
LEVEL OF TOXICITY
>1001
APPROXIMATE
CONCENTRATION FACTOR 0
NO
. k»| AMES RESPONSE
MCC
COMMENTS:
Hontoxic at 1001
*F« MM HAM ••••» Hw ECM Iw
bMEC " Mlnhmim dtacMTC CMWM
*SM T«M« t tof
86
-------
.PRIMARY
DATA TRANSITION FORM
ASSAY: Marine Fish
1IWI
MSULTS:
UMO |ECIO| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXKITV
SAMPLE: ¥-?
>10OT
NO
AMES RESPONSE
•EC*
COMMENTS: Not detectable
Hon toxic at 100V
•p« »• HAM M
*>MEC • MMm
Tabto
Hw ECU lor Nw motl n
oonccnltilkM |H
Mponihx PWMMMCWM b* Sated.
ldly
/ PRIMARY
DATA TRANSITION FORM
. lUHn* Fish
MSM-TS:
LDM (ECMI VALUE
CONCENTRATION FACTOR
LCVCt OP TOHiaTV
SAMPLE:
32 - S6S
COMMCNTS:
56S effluent produced 100( lethality.
•M RAM MM* «• tCM tar
ilraHaii IH
I* p*MHi«|.
87
-------
. PRIMARY
OAT* TRANSITION FORM
WE
A8SAy._JManne_Msh_
MAD: 1001
SAMPLE:
U-2
RESULTS:
LDSO (ECSOt VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXIC!!*"
Nontoxic at lOOi.
COMMENTS: Not detecUble
>100I
. |«| AMES RESPONSE
MEC
NO
*Fw NM RAM •»•( NM ECM lo> NM moil rnponctr*
bMEC - Minimum •llccll** conccnurtkxi IM iMpoiw* to
°8« Tibl* 1 Iw dflmlllMi ol lailclly
mM b* ItoM
PRIMARY
DATA TRANSITION FORM
WE
ASSAY: _
MAO: *00t
Marine Fish
SAMPLE:
C-2
RESULTS:
LDM |ECtO) VALUE
56 - 75*
taf| AMES RESPONSE
MEC
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITY*
COMMENTS:
LC50 - 70i
1001 toxic *t 100X effluent.
*Foi *• RAM MMf HM ECM to *w IM*| i
bMEC - Minimum ((taclln conc»«b>lhM IN
eBM T*M« 1 In dcllnllloii •! leilrfl,
-------
< PRIMARY
DATA TRANSITION FORM
ASSAY:
MAD: .
Marine Fish
100*
SAMPLE:
F-2
RESULTS:
LD60 |ECRO| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
>10QI
. |or| AMES RESPONSE
NEC
NO
COMMENTS:
Nontoxic at 100%
*For MM RAM *n*r Uw ECSO far lh» mml iMpomlM pwanwlw «rM b* H»l«d.
bMEC - Minimum •ItecMm eoncmtoallon IH iMpoiw* !•
°S«* Tcbl* 1 lot d*llnltlon ol toiklly I»«U
/ PRIMARY
DATA TRANSITION FORM
WE
ASSAY:
SAMPLE:
JflQ*
RESULTS:
LOW |ECBO| UAHIE
AMES RESPONSE
MEC
APPROXIMATE
CONCENTRATION FACTOR
OF TOXICITY
ND
COMMENTS: Non toxic
401 toxiclty at 1001
«F«, HM RAM HM* MM ECU Iw Hi* motl n»pon*tn pMWMtai wW b*
- Minimum •Itacll** eoncwihclloii III ratpom* h po»MI»»|
T»b4« 1 k» drflnHlMi ol
89
-------
/ PRIMARY
DATA TRANSITION FORM
. Marine Fish
ASSAY
MAD: IQOt
•AMPLE:
K-2
RESULTS:
IOM> (ECSOJ VALUE
LEVEL OF TOXICITY
. >100J
APPROXIMATE
CONCENTRATION FACTOR A
JUL
AMES RESPONSE
NEC
COMMEN1S:
NontoxU
*Fat *M RAM ••••» HM ECM tat *M «a*l
*SM TiM* 1 tat (MMttan •! laiklly
wW k« Itotad.
' PRIMARY
DATA TRANSITION FORM
MAO: 100X
SAMPtE:
1-2
RESULTS:
LDM IECHI VALUE* looi
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXICITV*
LOM '
. M AMR RESPONSE .
COMMENTS:
LC50 - 100*.
•F« «W RAM MM, ft, ECM tat ft. ,
90
-------
PRIMARY
DATA TRANSITION FORM
HE
ASSAY:
MAD:
Marine Fish
IQOt
•AMPLE:
N-2
RESULTS:
LOW IECSOI VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
32 - S6X
. tot) AMES RESPONSE
MEC
COMMENTS:
LC50 - 40*
Co^lete lethality at 100* effluent.
*F« MM RAM MUf *»• ECM lot tM mod raipond** p«wmlw wW bo Sited.
•MEC - Mlohnum dlKHM eoncmlftUiM | It iMponw Is pmllln).
•SM T»W» 1 lor 10OT
AMES RESPONSE
COMMENTS: Not d«t«CUbl« ' '• •'
20% Bortallty at 10M effluent.
•fa MM RAM MMf *• ECM tof KM MM! I*«K
knee - MtntaHMi altoeNiw oonewilraMan |H
•SM TiM* t tor (tettnTO** of toitolly toMl*.
mm b* Sstad.
91
-------
/ PRIMARY
DATA TRANSITION FORM
WE
ASSAY: H»rin» FUh
MAD: inn*
SAMPLE: B-2
RESULTS:
LOM |ECM| VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXIOTY0
>100I
HP
. |of| AMES RESPONSE
NEC
COMMENTS:
Non toxic at 1001.
'to, MM RAM Mur m» ECM lot *•• IWMI
bMEC - Mtnlmum
MM |M
*SM Tibto I tat iMhiHfcM e« toiktty fcttli.
wM to MM.
/ PRIMARY
DATA TRANSmON FORM
HE
ASSAY: _
MAD: 100t
Marine Ftsh
SAMPLE:
A-2
RESULTS:
LDM |ECM| VALVE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV
56 • 751
Nodcrtte
. fc»| AMES RESPONSE
MM*
COMMENTS:
LC50 - 60S tffluent
*F« «w RAM MMV »• (CSS tw »•
kMEC . Minimum •|!M«M lonmiiXI
ilH
•SM TIM* 1 tat d«mit>l«« •! todeNy hwto.
92
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PRIMARY
DATA TRANSITION FORM WE
ASSAY: Salmonella SAMPLE: l-2-jj R-2-5 U-2-5 X-2-5
n-<:-D b-^-b V-Z-5 Y-2-5
MAO: 5 nig/plate N-2-5 T-2-5 W 2-5 Z-2-5
RESULTS:
LD60 |EC80| VALUE* (or) AMES RESPONSE Negative
MEC"
APPROXIMATE
CONCENTRATION FACTOR 0
LEVEL OF TOXiciTV0 Nondetectable
COMMENTS:
All samples were tested it a maximum value of 1.0 ml/plate.
*Fof III* RAM •§••» Iht ECM lot llw motl iMponil** p«»n»lw will b* Milcd.
**MEC « Minimum tllvcllra conctnliillon I II rnpont* Is poilll«t|
°S»« T»bl« 1 l« (tollnllloo ol lonlclly !•»•!•.
PRIMARY
DATA TRANSITION FORM u£
ASSAV: Sa1mone11a SAMPLE: f~2^
MAO: _ Sag/plate
RESULTS:
LDSO (Ecsoi VALUE* |w| AMES RESPONSE Negative
MECb
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICITV Monde tec tjblg
COMMENTS:
Sample was tested at a maximum concentration (volume)
per plate of 500 pi.
*Fo> MM RAM MM* Mw ECM IM lit* mail fttpontlv* pvuiMlw «riH b« Nslxl
bMEC - Minimum •ItoolhM cenonkdloa III iciponu I* poilll*«|.
eS« TlW* 1 tof d*llnlllo« ol loilcMy !•*•!•.
93
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PRIMARY
DATA TRANSITION FORM WE
ASSAY: Salmonella
MAO: e, gq/plat»
RESULTS:
1050 IECMI VALUE*
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXICHY"
•AMPIF C-2-5
D-2-5
E-2-5
F-2-5
0
Nonde tec table
G-2-5
H-2-5
J-2-5
K-2-5
iwi AMES RESPONSE Negative
MECb
COMMENTS:
All samples employed 1.0 «1 as the wxinun concentration/plate.
•FW MM RAM ••»» MM ECM l« HM mMl m»B««t.« «o«miUi *M b« Httad.
bMEC - Mlnhnum •IlKNra eoncwHiBHeii IN t««p«"»» •• fo«Hlti|
C5« Table 1 lot d«llnlllon el le>ldly towl*.
94
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PRIMARY
OAT* TRANSITION FORM
HE
Salmonella
HAD: 5 nig/plate
SAMPLE:
B2
RESULTS:
LOCO (ECSOI VALUE
APPROXIMATE
CONCENTRATION FACTOR
LEVEL OF TOXKITV
Hondetectable
l«| AMES RESPONSE _Jegat1ve_
MEC
COMMENTS:
Haxlmum amount applied per plate Mas 1.0 ml.
•FO. *• RAM MUD MM ECM lor ttw motl nipontlM
•MEC - Minimum cHMltm ooncmtoHhMi III raipont* I* potW**):
•SM T«M* 1 l« (MMIkm •! leildly (ml*
PRIMARY
DATA TRANSITION FORM
ASSAY:
Salmonella
MAD: 5 Big/kg
SAMPLE:
A-2-
NESULTS:
LOW lEcsot VALUE"
APPROXIMATE
COMCENTRATION FACTOR
LEVEL OF TOXICITV
NO Nondetectable
l«l AMES RESPONSE
Jl
MEC
COMMENTS:
HaxlMun amount added per plate was 1.0 ml.
*Foi Hw RAM MMT MM ECM lot *M MMl iitponilM pttMMtar wM I
BMEC - MlnhMfm •Itaelb* conc*nW»lkm IN »«pom» h pe*IHv«|.
•••• Trtri* 1 lot dMnllton e< lonMly Iwrali.
95
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TECHNICAL REPORT DATA
(Please read /attractions on the reverse before completing!
1. REPORT NO.
EPA-600/7-80-079
3. RECIPIENT'S ACCESSION NO.
4. TITLE ANDSUBTITLE
Level 1 Biological Testing Assessment and
Data Formatting
6. REPORT DATE
April 1980
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
David J. Brusick
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME ANO ADDRESS
Litton Bionetics, Inc.
5516 Nicholson Lane
Kensington, Maryland 20795
10. PROGRAM ELEMENT NO.
INE623
11. CONTRACT/GRANT NO.
68-02-2681, Task 401
12. SPONSORING AGENCY NAME AND ADDRESS
EPA, Office of Research and Development
Industrial Environmental Research Laboratory
Research Triangle Park, NC 27711
13. TYPE OF REPORT AND PER,IOD COVERED
Task Final: 5/78-1/80
14. SPONSORING AGENCY CODE
EPA/600/13
16. SUPPLEMENTARY NOTES J.ERL-RTP project officer is Raymond G. Merrill, Mail Drop 62,
919/541-2557.
16. ABSTRACT
The report gives results of an examination of data from three pilot studies
including water effluent, fluidized-bed combustion, and coal gasification samples,
using a scheme to compare health effects and ecological bioassay assessment data.
The scheme is based on the assumption that each test method has a maximum dose
(concentration) which can be reliably applied and that effects based on fractions of
the maximum applicable dose (MAD) can be designated as degrees of toxicity. The
levels of toxicity based on fractions of the MAD are given as high, moderate, low,
and nondetectable (i.e. , no effect detected at the MAD).
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.IDENTIFIERS/OPEN ENDED TERMS
c. COS AT i Field/Group
Pollution
Bioassay
Toxicity
Waste Water
Combustion
Fluidized Bed Processing
Coal Gasification
Pollution Control
Stationary Sources
13B
06A
06T
2 IB
13H,07A
3. DISTRIBUTION STATEMENT
Release to Public
19. SECURITY CLASS (This Report/
Unclassified
21. NO. OF PAGES
100
20. SECURITY CLASS (This page I
Unclassified
22. PRICE
If A Fotm 2220-1 (»-7J)
96
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