United States
Environmental Protection
Agency
Environmental Monitoring
and Support Laboratory
PO Box 15027
Las Vegas NV 89114
EPA 600 3-79-114
December 1979
Research and Development
Distribution of
in
Color*
Lakes
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RESEARCH REPORTING SERIES
Research reports of trie Office of Research and Development. U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad categories
were established to facilitate further development and application of environmental
technology. Elimination of traditional grouping was consciously planned to foster
technology transfer and maximum interface in related fields. The nine series are:
1. Environmental Health Effects Research
2. Environmental Protection Technology
3. Ecological Research
4. Environmental Monitoring
5. Socioeconomic Environmental Studies
6. Scientific and Technical Assessment Reports (STAR)
7. Interagency Energy—Environment Research and Development
8. "Special" Reports
9. Miscellaneous Reports
This report has been assigned to the ECOLOGICAL RESEARCH series. This series
describes research on the effects of pollution on humans,plant and animal species, and
materials. Problems are assessed for their long-and short-term influences, investiga-
tions include formations, transport, and pathway studies to determine the fate of
pollutants and their effects. This work provided the technical basis for setting standards
to minimize undesirable changes in living organisms in the aquatic, terrestrial, and
atmospheric environments.
This document is available lo the public through the National Technical Information
Service, Springfield, Virginia 22161
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EPA-600/3-79-114
December 1979
DISTRIBUTION OF PHYTOPLANKTON IN COLORADO LAKES
by
M. K. Morris*, W. D. Taylor, L. R. Williams,
S. C. Hern, V. W. Lambou, and F. A. Morris*
Water and Land Quality Branch
Monitoring Operations Division
Environmental Monitoring and Support Laboratory
Las Vegas, Nevada 89114
*Department of Biological Sciences
University of Nevada, Las Vegas
'as Vegas, Nevada 89154
ENVIRONMENTAL MONITORING AND SUPPORT LABORATORY
OFFICE OF RESEARCH AND DEVELOPMENT
U.S. ENVIRONMENTAL PROTECTION AGENCY
LAS VEGAS, NEVADA 89114
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DISCLAIMER
This report has been reviewed by the Environmental Monitoring and
Support Laboratory-Las Vegas, U.S. Environmental Protection Agency, and
approved for publication. Mention of trade names or commercial products does
not constitute endorsement or recommendation for use.
n
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FOREWORD
Protection of the environment requires effective regulatory actions
which are based on sound technical and scientific information. This
information must include the quantitative description and linking of
pollutant sources, transport mechanisms, interactions, and resulting effects
on man and his environment. Because of the complexities involved, assessment
of specific pollutants in the environment requires a total systems approach
which transcends the media of air, water, and land. The Environmental
Monitoring and Support Laboratory-Las Vegas contributes to the formation and
enhancement of a sound monitoring data base for exposure assessment through
programs designed to:
• develop and optimize systems and strategies for monitoring
pollutants and their impact on the environment
•demonstrate new monitoring systems and technologies by
applying them to fulfill special monitoring needs of the
Agency's operating programs
This report presents the species and abundance of phytoplankton in the
13 lakes sampled by the National Eutrophication Survey in the State of
Colorado, along with results from the calculation of several commonly used
biological indices of water quality and community structure. These data can
be used to biologically characterize the study lakes, and as baseline data
for future investigations. This report was written for use by Federal,
State, and local governmental agencies concerned with water quality analysis,
monitoring, and/or regulation. Private industry and individuals similarly
involved with the biological aspects of water quality will find the document
useful. For further information contact the Water and Land Quality Branch,
Monitoring Operations Division.
,
George 9. -Morgan
Director
Environmental Monitoring and Support Laboratory
Las Vegas
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CONTENTS
Page
Foreword ............................... iii
Introduction ............................. 1
Materials and Methods ........ • • .............. 2
Lake and Site Selection ..................... 2
Sample Preparation ....................... 2
Examination ........................... 3
Quality Control ......................... 4
Results ............................... 5
Nygaard1 s Trophic State Indices ................. 5
Palmer's Organic Pollution Indices ..... .......... 5
Species Diversity and Abundance Indices ............. 7
Species Occurrence and Abundance ................ 9
Literature Cited ........................... 1°
Appendix A. Phytoplankton Species list for the State
of Colorado ................. ...... 11
Appendix R. Summary of Phytoplankton Data
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INTRODUCTION
The collection and analysis of phytoplankton data were included in the
National Eutrophication Survey in an effort to determine relationships between
algal characteristics and trophic status of individual lakes.
During spring, summer, and fall of 1975, the Survey sampled 156 lakes in
11 States. Over 450 algal species and varieties were identified and
enumerated from the 430 water samples examined.
This report presents the species and abundance of phytoplankton in the
13 lakes sampled in the State of Colorado (Table 1). The Nygaard's Trophic
State (Nygaard 1949), Palmer's Organic Pollution (Palmer 1969), and species
diversity and abundance indices are also included.
TABLE 1. LAKES SAMPLED IN THE STATE OF COLORADO
STORET No.
0801
0802
0803
0804
0805
0806
0807
0808
0809
0810
0811
0812
Lake Name
Barker Lake
Barr Lake
Blue Mesa Reservoir
Cherry Creek
Cucharas Reservoir
Dillon Lake
Grand Lake
Green Mountain Reservoir
Hoi brook Lake
Lake Meredith
Milton Reservoir
Navajo Lake
County
Boulder
Adams
Gunnison,
Arapahoe
Huerfano
Summit
Grand
Summit
Otero
Crowl ey
Weld
Archuleta
Montrose
(San Juar
J — M PJ,
0813 Shadow Mountain Lake Grand
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MATERIALS AND METHODS
LAKE AND SITE SELECTION
Lakes and reservoirs included in the Survey were selected through
discussions with State water pollution agency personnel and U.S. Environmental
Protection Agency Regional Offices (U.S. Environmental Protection Agency
1975). Screening and selection strongly emphasized lakes with actual or
potential accelerated eutrophication problems. As a result, the selection was
1imited to lakes:
(1) impacted by one or more municipal sewage treatment plant outfalls
either directly into the lake or by discharge to an inlet tributary
within approximately 40 kilometers of the lake;
(2) 40 hectares or larger in size; and
(3) with a mean hydraulic retention time of at least 30 days.
Specific selection criteria were waived for some lakes of particular State
interest.
Sampling sites for a lake were selected based on available information on
lake morphometry, potential major sources of nutrient input, and on-site
judgment of the field limnologist (U.S. Environmental Protection Agency 1975).
Primary sampling sites were chosen to reflect the deepest portion of each
major basin in a test lake. Where many basins were present, selection was
guided by nutrient source information on hand. At each sampling site, a
depth-integrated phytoplankton sample was taken. Depth-integrated samples
were uniform mixtures of water from the surface to a depth of 15 feet
(4.6 meters) or from the surface to the lower limit of the photic zone
representing 1 percent of the incident light, whichever was greater. If the
depth at the sampling site was less than 15 feet (4.6 meters), the sample was
taken from just off the bottom to the surface. Normally, a lake was sampled
three times in 1 year, providing information on spring, summer, and fall
conditions.
SAMPLE PREPARATION
To preserve the sample 4 milliliters (ml) of Acid-Lugol's solution
(Prescott 1970) were added to each 130-ml sample from each site at the time of
collection. The samples were shipped to the Environmental Monitoring and
Support Laboratory, Las Vegas, Nevada, where equal volumes from each site
-------
were mixed to form two 130-ml composite samples for a given lake. One
composite sample was put into storage and the other was used for the
examination.
Prior to examination, the composite samples were concentrated by the
settling method. Solids were allowed to settle for at least 24 hours prior to
siphoning off the supernate. The volume of the removed supernate and the
volume of the remaining concentrate were measured and concentrations
determined. A small (8-ml) library subsample of the concentrate was then
taken. The remaining concentrate was gently agitated to resuspend the
plankton and poured into a capped, graduated test tube. If a preliminary
examination of a sample indicated the need for a more concentrated sample, the
contents of the test tube were further concentrated by repeating the settling
method. Final concentrations varied from 15 to 40 times the original.
Permanent slides were prepared from concentrated samples after analysis
was complete. A ring of clear Karo® corn syrup with phenol (a few crystals of
phenol were added to each 100 ml of syrup) was placed on a glass slide. A
drop of superconcentratefrom the bottom of the test tube was placed in the
ring. This solution was thoroughly mixed and topped with a coverglass. After
the syrup at the edges of the coverglass had hardened, the excess was scraped
away and the mount was sealed with clear fingernail polish. Permanent diatom
slides were prepared by drying sample material on a coverglass, heating in a
muffle furnace at 400° C for 45 minutes, and mounting in Hyrax®. Finally, the
mounts were sealed with clear fingernail polish.
Backup samples, library samples, permanent sample slides, and
Hyrax-mounted diatom slides are being stored and maintained at the
Environmental Monitoring and Support Laboratory-Las Vegas.
EXAMINATION
The phytoplankton samples were examined with the aid of binocular
compound microscopes. A preliminary examination was performed to precisely
identify and list all forms encountered. The length of this examination
varied depending on the complexity of the sample. An attempt was made to find
and identify all of the forms present in each sample. Often forms were
observed which could not be identified to species or to genus. Abbreviated
descriptions were used to keep a record of these forms (e.g., lunate cell,
blue-green filament, Navicula #1). Diatom slides were examined using a
standard light microscope. If greater resolution was essential to accurately
identify the diatoms, a phase-contrast microscope was used.
After the species list was compiled, phytoplankton were enumerated using
a Neubauer Counting Chamber with a 40X objective lens and a 10X ocular lens.
All forms within each field were counted. The count was continued until a
minimum of 100 fields had been viewed, or until the dominant form had been
observed a minimum of 100 times.
^Registered trademark
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QUALITY CONTROL
Project phycologists performed internal quality control intercomparisons
regularly on 7 percent of the species identification and counts. Although an
individual had primary responsibility for analyzing a sample, taxonomic
problems were discussed among the phycologists.
Additional quality control checks were performed on the Survey samples by
Dr. G. W. Prescott of the University of Montana at the rate of 5 percent.
Quality control checks were made on 75 percent of these samples to verify
species identifications while checks were made on the remaining 25 percent of
the samples to verify genus counts. Presently, the agreement between quality
control checks for species identification and genus enumerations is
satisfactory.
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RESULTS
A phytoplankton species list for the State is presented in Appendix A.
Appendix B summarizes all of the phytoplankton data collected from the State
by the Survey. The latter is organized by lake, and includes an alphabetical
phytoplankton species list with concentrations for individual species given by
sampling date. Results from the application of several indices are presented
(Nygaard's Trophic State, Palmer's Organic Pollution, and species diversity
and abundance). Each lake has been assigned a four-digit STORET number.
[STORET (STOrage and RETrieval) is the U.S. Environmental Protection Agency's
computer system which processes and maintains water quality data.) The first
two digits of the STORET number identify the State; the last two digits
identify the lake.
NYGAARD'S TROPHIC STATE INDICES
Five indices devised by Nygaard (1949) were proposed under the assumption
that certain algal groups are indicative of levels of nutrient enrichment.
These indices were calculated in order to aid in determining the surveyed
lakes' trophic status. As a general rule, Cyanophyta, Euglenophyta, centric
diatoms, and members of the Chlorococcales are found in waters that are
eutrophic (rich in nutrients), while desmids and many pennate diatoms
generally cannot tolerate high nutrient levels and so are found in
oligotrophic waters (poor in nutrients).
In applying the indices to the Survey data, the number of taxa in each
major group was determined from the species list for each sample. The ratios
of these groups give numerical values which can be used as a biological index
of water richness. The five indices and the ranges of values established for
Danish lakes by Nygaard for each trophic state are presented in Table 2. The
appropriate symbol, (E) eutrophic and (0) oligotrophic, follows each
calculated value in the tables in Appendix B. A question mark (?) following a
calculated value in these tables was entered when that value was within the
range of both classifications.
PALMER'S ORGANIC POLLUTION INDICES
Palmer (1969) analyzed reports from 165 authors and developed algal
pollution indices for use in rating water samples with high organic pollution.
Two lists of organic-pollution-tolerant forms were prepared, one containing
20 genera, the other, 20 species (Tables 3 and 4). Each form was assigned a
pollution index number ranging from 1 for moderately tolerant forms to 6 for
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TABLE 2. NYGAARD'S TROPHIC STATE INDICES ADAPTED FROM HUTCHINSON (1967)
Index
Calculation
Oligotrophi'c Eutrophi'c
Myxophycean
Chlorophycean
Diatom
Euglenophyte
Compound
Myxophyceae
Desmideae
Chlorococcales
Desmideae
Centric Diatoms
Pennate Diatoms
Eugjenophyta
Myxophyceae + Chlorococcales
Myxophyceae + Chlorococcales +
0.0-0.4
0.0-0.7
0.0-0.3
0.0-0.2
0.0-1.0
0.1-3.0
0.2-9.0
0.0-1.75
0.0-1.0
1.2-25
Centric Diatoms + Euglenophyta
Desmideae
TABLE 3. ALGAL GENUS POLLUTION INDEX
(Palmer 1969)
TABLE 4. ALGAL SPECIES POLLUTION
INDEX (Palmer 1969)
Genus
Anacystis
Ankistrodesmus
Chlamydomonas
Chlorella
Closterium
Cyclotella
Euglena
Gomphonema
Lepocinclis
Melosira
Micractinium
Navicula
Nitzschia
Oscillatoria
Pandorina
Phacus
Phormidium
Scenedesmus
Stigeoclonium
Synedra
Pollution
Index
1
2
4
3
1
1
5
1
1
1
1
3
3
5
1
2
1
4
2
2
Species
Ankistrodesmus falcatus
Arthrospira jenneri
Chi ore! la vulgaris
Cyclotella meneghiniana
Euglena gracilis
Euglena viridis
Gomphonema parvulum
Melosira varians
Navicula cryptocephala
Nitzschia acicularis
Nitzschia pjilea
Oscillatoria chlorina
Oscillatoria limosa
Oscillatoria pjrinceps
Oscillatoria putrida
Oscillatoria tenuis
Pandorina morum
Scenedesmus quadricauda
Stigeoclonium tenue
Synedra ulna
Pollution
Index
3
2
2
2
1
6
1
2
1
1
5
2
4
1
1
4
3
4
3
3
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extremely tolerant forms. Palmer based the index numbers on occurrence
records and/or where emphasized by the authors as being especially tolerant of
organic pollution.
In analyzing a water sample, any of the 20 genera or species of algae
present in concentrations of 50 per milliliter or more are recorded. The
pollution index numbers of the algae present are totaled, providing a genus
score and a species score. Palmer determined that a score of 20 or more for
either index can be taken as evidence of high organic pollution, while a score
of 15 to 19 is taken as probable evidence of high organic pollution. Lower
figures suggest that the organic pollution of the sample is not high, that the
sample is not representative, or that some substance or factor interfering
with algal persistence is present and active.
SPECIES DIVERSITY AND ABUNDANCE INDICES
"Information content" of biological samples is being used commonly by
biologists as a measure of diversity. Diversity in this connection means the
degree of uncertainty attached to the specific identity of any randomly
selected individual. The greater the number of taxa and the more equal their
proportions, the greater the uncertainty, and hence, the diversity (Pielou
1966). There are several methods of measuring diversity, e.g., the formulas
given by Brillouin (1962) and Shannon and Weaver (1963). The method which is
appropriate depends on the type of biological sample on hand.
Pielou (1966) classifies the types of biological samples and gives the
measure of diversity appropriate for each type. The Survey phytoplankton
samples are what she classifies as larger samples (collections in Pielou 's
terminology) from which random subsamples can be drawn. According to Pielou,
the average diversity per individual (H) for these types of samples can be
estimated from the Shannon-Wiener formula (Shannon and Weaver 1963):
1=1
where P is the proportion of the ith taxon in the sample, which is calculated
from ni/N; n^ is the number of individuals per milliliter of the ith
taxon; N is the total number of individuals per ml; and S is the total number
of taxa. However, Basharin (1959) and Pielou (1966) have pointed out that H
calculated from the subsample is a biased estimator of the sample H, and if
this bias is to be accounted for, we must know the total number of taxa
present in the sample since the magnitude of this bias depends on it.
Pielou (1966) suggests that if the number of taxa in the subsample falls
only slightly short of the number in the larger sample, no appreciable error
will result in considering S, estimated from the subsample, as being equal to
the sample value. Even though considerable effort was made to find and
identify all taxa, the Survey samples undoubtedly contain a fair number of
rare phytoplankton taxa which were not encountered.
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In the Shannon-Wiener formula, an increase in the number of taxa and/or
an increase in the evenness of the distribution of individuals among taxa will
increase the average diversity per individual from its minimal value of zero.
Sager and Hasler (1969) found that the richness of taxa was of minor
importance in determination of average diversity per individual for
phytoplankton and they concluded that phytoplankton taxa in excess of the 10
to 15 most abundant ones have little effect on H. This was verified by our
own calculations. Our counts are in number per milliliter and since
logarithms to the base 2 were used in our calculations, H is expressed in
units of bits per individual. When individuals of a taxon were so rare that
they were not counted, a value of 1/130 per milliliter or 0.008 per milliliter
was used in the calculations since at least one individual of the taxon must
have been present in the collection.
A Survey sample for a given lake represents a composite of al 1
phytoplankton collected at different sampling sites on the lake during a given
sampling period. Since the number of samples (M) making up a composite is a
function of both the complexity of the lake sampled and its size, it should
affect the richness-of-taxa component of the diversity of our phytoplankton
collections. The maximum diversity (MaxH) (i.e., when the individuals are
distributed among the taxa as evenly as possible) was estimated from Iog2 S
(Pielou 1966) while the minimum diversity (MinH), was estimated from the
formula:
MinH . .
given by Zand (1976). The total diversity (D) was calculated from HN (Pielou
1966). Also given in Appendix B are L (the mean number of individuals per
taxa per milliliter) and K (the number of individuals per milliliter of the
most abundant taxon in the sample).
The evenness component of diversity (J) was estimated from H/MaxH
(Pielou 1966). Relative evenness (RJ) was calculated from the formula:
D1 H-MinH
KJ =
MaxH-MinH
given by Zand (1976). Zand suggests that RJ be used as a substitute for both
J and the redundancy expression given by Wilhm and Dorris (1968). As pointed
out by Zand, the redundancy expression given by Wilhm and Dorris does not
properly express what it is intended to show, i.e., the position of H in the
range between MaxH and MinH. RJ may range from 0 to 1; being 1 for the most
even samples and 0 for the least even samples.
Zand (1976) suggests that diversity indices be expressed in units of
"sits", i.e., in logarithms to base S (where S is the total number of taxa in
the sample) instead of in "bits", i.e., in logarithms to base 2. Zand points
out that the diversity index in sits per individual is a normalized number
ranging from 1 for the most evenly distributed samples to 0 for the least
evenly distributed samples. Also, it can be used to compare different
samples, independent of the number of taxa in each. The diversity in bits per
8
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individual should not be used in direct comparisons involving various samples
which have different numbers of taxa. Since MaxH equals log S, the expression
in sits is equal to logs S, or 1. Therefore diversity in sits per
individual is numerically equivalent to J, the evenness component for the
Shannon-Wiener formula.
SPECIES OCCURRENCE AND ABUNDANCE
The alphabetic phytoplankton species list for each lake, presented in
Appendix B, gives the concentrations of individual species by sampling date.
Concentrations are in cells, colonies, or filaments (CEL, COL, FIL) per
milliliter. An "X" after a species name indicates that the species identified
in the preliminary examination was in such a low concentration that it did not
appear in the count. A blank space indicates that the organism was not found
in the sample collected on that date. Column S is used to designate the
examiner's subjective opinion of the five dominant taxa in a sample, based
upon relative size and concentration of the organism. The percent column (%C)
presents, by abundance, the percentage composition of each taxon.
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LITERATURE CITED
Basharin, G. P. 1959. On a statistical estimate for the entropy of a
sequence of independent random variables, pp. 333-336. _I_n: Theory of
Probability and Its Applications (translation of "Teoriya Veroyatnosei i
ee Premeneniya"). N. Artin (ed). 4. Society for Industrial and
Applied Mathematics, Philadelphia.
Brillouin, L. 1962. Science and Information Theory (2nd ed.). Academic
Press, New York. 351 pp.
Hutchinson, G. E. 1967. A Treatise on Limnology. II. Introduction to Lake
Biology and the Limnoplankton. John Wiley and Sons, Inc., New York.
1,115 pp.
Nygaard, G. 1949. Hydrobiological studies of some Danish ponds and lakes.
II. (K danske Vldensk^Selsk.) Biol. Sci. 7:293.
Palmer, C. M. 1969. A composite rating of algae tolerating organic
pollution. J. Phycol. 5:78-82.
Pielou, E. C. 1966. The measurement of diversity in different types of
biological collections. J. Theor. Biol. 13:131-144.
Prescott, G. W. 1970. How to Know the Freshwater Algae. William C. Brown
Company, Dubuque. 348 pp.
Sager, P. E., and A. D. Hasler. 1969. Species diversity in lacustrine
phytoplankton. I. The components of the index of diversity
from Shannon's formula. Amer. Natur. 103(929):51-59.
Shannon, C. E., and W. Weaver. 1963. The Mathematical Theory of Commu-
nication. University of Illinois Press, Urbana. 117 pp.
U.S. Environmental Protection Agency. 1975. National Eutrophication Survey
Methods 1973-1976. Working Paper No. 175. Environmental Monitoring and
Support Laboratory, Las Vegas, Nevada, and Corvallis Environmental
Research Laboratory, Corvallis, Oregon. 91 pp.
Wilhm, V. L., and T. C. Dorris. 1968. Biological parameters for water
quality criteria. Bio-Science. 18:477.
Zand, S. M. 1976. Indexes associated with information theory in water
quality. J. Water Pollut. Contr. Fed. 48(8):2026-2031.
10
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APPENDIX A
PHYTOPLANKTON SPECIES LIST FOR THE STATE OF COLORADO
11
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Actinastrum gracilimum
Andbaena planetonica
Anabaenopeie airaularie
Ankistrodesmue faleatus
v. aeieularie
Ankistrode emus faleatus
v. mirabilis
Aphoniaomenon floe-aquae
Aphanoeapsa
Asterionella fomasa
Binuelearia
Carteria
Ceratium hirundinella
f. seottieum
Chi orogonium
Chrooeoecus dispersus
Chroocoeeus limnetieus
Closteriwn
Coccaneis plaeentula
Coelastrwn microporum
Coelastrum sphaerieum
Coelosphaerium 'k.uetzi.ng'ianum
Coelosphaerium pallidum
CosmarLum
Cryptomonas erosa
Cryptanonas marseonii
Cryptomonae i°eflexa
Cryptomonae roetrata
Cyelotella bodaniaa
Cyclotella ccmta
Cyelotella meneghiniana
Cymatopleura elliptiea
Cymatopleura solea
Cymbella mexicana
Vactylococcops-is
Diatoma tenue
V. elongatum
Diatoma vulgare
Dictyoephaer>ium pulehellum
Dinobryon bavaricum
Dinobryon cylindricum
Dinobryon divergene
Entomoneis alata
Epithemia
Euglena oxyur>is
V. minor
Fragilaria eonstruens
Fragilaria crotonensis
Fragilaria leptostauron
Glenodinium edax
Gamphonema olivaeeum
Gomphoephaeria
Gymnodinium
Gyros igma
Hannaea ar>eus
Hantssehia
Lagerheimia
Lyngbya
Mai I cmonas
Melosira dietans
Meloeira granulata
v. angustissima
Meloeira italica
Melosira varians
Meriemopedia minima
Mer>ismopedia tenuissima
Mieraatinium pus ilium
Mieroeystis aeruginosa
Mieroaystie ineerta
Mougeotia
Naviaula amphibola
Navieula euspidata
Nitssehia longissima
v. reversa
Oedogonium
Ooeystis
Oseillator*ia limnetiea
Pediastrum boryanum
Pediaetnm duplex
Pediastrum duplex
v. elathratum
Pediastrum duplex
v. retiaulatum
Pediastrum simplex
v. duodenarium
Phaeus megalopsis
Phacus pleuroneetes
Phaeus pseudonordstedtii
Phaeus tortus
Phomidium mueicola
Pi euros igma
Raphidiopsis
Rhoieosphenia curvata
Rhopalodia gibba
Seenedesmus aeuminatus
Seenedesmus armatus
Seenedesmus dimorphus
Seenedesmus quadricauda
Sehroederia judayi
Sehroederia eetigera
12
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Spematozoopsis Synedra ulna
Sphaerocystie sohroeteri Tabellaria feneetrata
Spirulina Tetraedron minimum
Spondylos-uan planwn Tetrviedran mutiaum
Staurastmm Tetrastrum elegans
Stephanadiscus dubiue Tetraetrum staurogeniaeforme
Stephanodisaus niagarae Trachelcmonas intermedia
Synedra cyclcpum Trachelomonas planetonica
13
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APPENDIX B. SUMMARY OF PHYTOPLANKTON DATA
This appendix was generated by computer. Because it was only possible to
use upper case letters in the printout, all scientific names are printed in
upper case and are not italicized.
The alphabetic phytoplankton lists include taxa without species names
(e.g., EUNOTIA, EUNOTIAtfl, FLAGELLATE, FLAGELLATES, MICROCYSTIS INCERTA ?,
CHLOROPHYTAN COCCOID CELLED COLONY). When species determinations were not
possible, symbols or descriptive phrases were used to separate taxa for
enumeration purposes. Each name on a list, however, represents a unique
species different from any other name on the same list, unless otherwise
noted, for counting purposes.
Numbers were used to separate unidentified species of the same genus. A
generic name listed alone is also a unique species. A question mark (?) is
placed immediately after the portion of a name which was assigned with
uncertainty. Numbered, questioned, or otherwise designated taxa were
established on a lake-by-lake basis; therefore NAVICULA #2 from lake A cannot
be compared to NAVICULA #2 from lake B. Pluralized categories (e.g.,
FLAGELLATES, CENTRIC DIATOMS, SPP.) were used for counting purposes when taxa
could not be properly differentiated on the counting chamber.
14
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LAKE NAMEt BARKER
STORET NUMBER I 0801
NYGAARD TROPHIC STATE INDICES
DATE OK 07 75 09 26 75 10 10 75
MYXOPHYCEAN
CHLOROPHYCEAN
EUGLENOPHYTE
DIATOM
COMPOUND
1.00 E
0/01 0
0/01 ?
0.50 t
3.00 E
2.00 E
1.00 E
0/03 T
l.frO E
5.00 E
01/0 E
03/0 E
0.25 E
0.50 E
07/0 E
PALMFP'9 ORGANIC POLLUTION INDICES
DATE OS 07 75 08 36 75 10 10 75
GCNUa
SPECIES
01
00
01
00
10
00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 05 07 75 08 26 75 10 10 75
AVERAGE DIVERSITY
NUMBER OF TAXA
NUMBER OF SAMPLES COMPOSITED
MAXIMUM DIVERSITY NAXH
MINIMUM DIVERSITY MINH
TOTAL DIVERSITY
TOTAL NUMBER OF INDIVIDUALS/ML
EVENNt.86 COMPONENT
RELATIVE EVENNESS
MEAN NUMBER OF INDIVIDUALS/TAX*
NUMBER/ML OF MOST ABUNDANT TAXON
H
8
M
XH
NH
D
N
J
RJ
L
K
1.25
16.00
2.00
4.00
0.02
14330,00
11464,00
0.11
0.31
716. SO
9013.00
2.00
10.00
2.00
J.32
0.04
5114.00
2557.00
0.60
O.SO
255.70
1381.00
0.99
15.00
2,00
3.91
0.03
7608,15
7685.00
0.25
0.25
512.33
6478.00
15
-------
LAKE NAMKl BARKER
STOHET NUMBERI 0801
TAX*
ANABAENA
ANABAENOPRI8 CIRCULAR!*
A8TEPIONELLA FORMOSA
CARTERIA
CHtAIYOOMOINlS T
CHROOMONAS T
COCCONEI8 PLACrHTUtA
C08MARIUM
CRYPTOHONAS
CRYPTOMONAS EROSft
CRYPTOMONA8 MARSSONII
DACTYLOCOCCOPSIS
DICTYORPNACH1UM PULCHELLUM
DINOBRION ClblHCBICllM
FRAGILARIA COHSTRUENS
rRAQZLARIA CHOTONgNSId
OOMPHONCMA OLIViCCUM
CtMNOOIKIUK
HANNAE* ARCUS
NALLOMONA8
NALLOHONA8 ?
MKL08IRA DISTAN8
XRLOSIRA GRANULATA
V. ANCURTISOIMA
XrLO«IP» ITALICA
ORCILLATORIA
prniASTRUK Bopyjmu"
PID1ABTRUM SIMPLEX
V. DUODENARIUH
«CHRQRDCRIA 8ETIGCRA
•PONDYL08IUM PLANUM
TRACHELOMONA8
TOTAL
OB OT
0« It T«
10 10 IS
I ALGAL
I UNITS
FORM 1* 771
X
X
0.91 101
0.9| 101
O..*l It
1
1 *
1
S.ll M>
0.11 It
1 X
1 x
1
0.61 7?
t.ll 711
71,6 901]
ALOAL
UNIT8
S *C PER ML
ii a. oi si
*
i
4
I X
2.0| 11
10,0
2.0
34,0
4.0
4.0
2»*
»1
X
im
102
102
uiji.oi sea
i t i
ALOAL
UNIT8
B %C PtR ML
1
4
S
1
2
M.J
J.7
O.I
4.9
4,9
Itt
X
(471
20*
4J
X
X
X
X
174
174
121
X
X
0.11 42
1
O.SI 42
I14M a>«7 70S
16
-------
LAKE NAMEt BARR LAKE
8TORET NUMBERl 0802
NYGAARD TROPHIC SIATE INDICES
DATE 05 05 75 08 26 75 10 10 75
MYXOPHYCEAN
CHLOROPHYCEAN
EUGLENOPHYTB
DIATOM
COMPOUND
0/0 0
01/0 E
0/01 T
0/02 ?
01/0 E
OS/0 E
02/0 G
0/07 7
01/0 E
OB/0 E
03/0 E
02/0 C
0.20 ?
1.00 E
08/0 E
PALMER'S ORGANIC POLLUTION INDICES
DATE 08 05 75 08 26 75 10 10 75
GENUS
SPECIES
00
00
01
00
05
00
SPECIES DIVRPSTTY AND ABUNDANCE INDICES
DATE OS 05 75 08 26 75 10 10 75
AVERAGE DIVERSITY
NUMBER Of TAXA
NUMBER OF SAMPLES COMPOSITED
MAXIMUM DIVERSITY MAXH
MINIMUM DIVERSITY MJNH
TOTAL DIVERSITY
TOTAL HUMBKP OF INDIVIDUALS/ML
EVENNESS COMPONENT
RELATIVE EVENNESS
MEAN NUMBER OF INDIVIDUALS/TAX*
NUMBER/ML OF MOST ABUNDANT TAXON
H
8
M
XH
NH
D
N
J
RJ
L
K
1.79
7.00
2.00
2.81
0.03
4204.71
2349.00
O.C4
O.C4
JJ5.57
1149.00
0.46
9.00
2.00
3,17
0.03
1612.30
3505.00
0.15
0.14
189.44
3265.00
1,39
12.00
2.00
3.88
0.09
1948.78
1402.00
0.39
0.38
116.83
1012.00
17
-------
LAKE HAHEl §»HR LAKE
•TOP.FT NUPHERI 0*0]
TAM
ANABAENA
APNANIEOHENON FLOS-AQUAE
CHROONONAS T
CRYPTOMONA* EROSA
CRYPTOHOHAS HARHSCJKII
cm
D1CTYOSPHAKRIUM PIILCHF.LLUM
COKTINUEO
OS OS 78
01 It 7»
10 10 IS
GOMPHOSPHAERIA
MCLOSIRA
MELOMKA GHANULATA
V. ANGUSTISaiMA
NICROClSTIi AERUGINOSA
NITZSCHIA II
NITfSCHIA 12
OOCHII8
PRDIA8TP.UH DUPLEX
PHORKiniUH MUCICOLA
HCENEOESMUS ACUMINATHS
ICHROEDERIA SETIGCRA
STEPNANODISCUS
STNCDKH
TOTAL
1 AUKAL 1 AL6AL 1 ALGAL
1 UNITS 1 UNITS I UNITS
FORM II «C PER Ml. IS %C PER ML IS »C PER ML
PIL 1 1 1 III XIII X
riL 1 1 1 IIH3.JI 12«S 11172.21 1012
CEL I4l4t.fl H4t I I
C1L 1112*.'! $79
CEL 111 «.7I IS7
CEL III 2.2) S3
COL
CEL
COL
CEL
CEL
COL
CEI.
CEL
CEL
COL
COL
COL
CEL
CEL
CEI,
2
1
1
1J.J
K
1IJ
X
1
2
1
4
2.7
2.7
1.4
X ISI S.ll 71
1
1
I
X 1
1
»« 1
11
1
X 1
t« 12
1
14
|
X 1
41 1
1
1
|
I
11. 1
S.k
».*
X
X
IS*
7S
X
7t
X
X
X
9144 ISO! 1402
18
-------
LAKE NAMtl BLUE HEBA PES.
STORET NUMBERI 0003
NYGAARD TROPHIC STATE INDICES
DATE 08 25 75 09 30 75
MYXOPHYCERN 2.00 E 01/0 E
CHLQROPHYCEAN 3.00 E 01/0 C
EUGLENOPHYTE 0/05 ? 0/02 1
DIATOM 0,33 t 0/dl 1
COMPOUND 6.00 E 02/0 E
PALMER'S OPGANIC POLLUTION INDICES
DATE OB 25 75 09 30 75
GENUS 01 00
SPECIES 00 00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE OK 25 75 09 30 75
AVERAGE DIVERSITY H 2.13 0.59
NUMBER OF TAXA S 12.00 5.00
NUMBER OF SAMPLES COMPOSITED M 6.00 6.00
MAXIMUM DIVERSITY MAXH 3.SB 2.32
MINIMUM DIVERSITY MINN 0.06 0.01
TOTAL DIVERSITY D 5249.12 2838.49
TOTAL NUMBER OF INDIVIDUALS/ML N 2476.00 481t.OO
KVENNESS COMPONENT J 0,59 0.25
RELATIVE EVENNESS RJ 0.59 0.26
MEAN NUMBER OK INDIVIDUAL8/TAXA b 206.33 962.20
NUMBER/ML OF MUST ABUNDANT TAXON K 1324.00 4391.00
19
-------
LAKE NAMEl IIUF *ES» RKS.
ITORET HUMBERI 0193
TAX*
APHUHIIONCKON rt,os-ACUA«:
ASTENIONELLA FORMOSA
CHROOHUNAS J
CRmOMONAS ER08A
CrMBILLA
rRACILARU CROTONCNStS
NJCRPCmU INCERTI
OUCISTIB
•CHROtDIRIA 1ETIGCRA
mURAITRUM
SirPHANDDISCUS
TETRAEDROM MUTICUH
TOTAL
COKTIMUKD
01 IS 75
0* 10
I
I
IS
1C
AtflAL
UNITS
PER ML
I
IS
ALQAb
UNITS
%C PER ML
TIL Mill.91 IJ24 11191.11 4)91
CCL
CCL
CtL
CCL
CCL
COL
CCL
CCL
eti
CCL
CCL
I I
lllil.tl
111 4.11
I I I
ISI 4.«1
141 4,«l
1
I
4.«l
1.11
II
461
210
I
119
its
X
119
X
SI
SI
747*
I I I
111 J.»l
141 1.91
I I I
111 1.91
IS
1.0
91
91
47
4111
20
-------
LAKE NAMEl CHERRY CREEK
STORET NUMBERI 0804
NYGAARD TROPHIC STATE INDICES
DATE 05 07 75 09 22 75 10 09 75
MYXQPHYCEAN
CHLOROPHYCEAM
GUGIKNOPHYTE
DIATOM
COMPOUND
0/0 0
01/0 E
0/01 »
0.17 1
02/0 E
1.00 E
0/01 0
1.00 E
0.23 T
5.00 E
0.50 B
1.29 E
0.71 E
0.3) E
3.50 G
PALMER*S CRUAKIC POLLUTION INDICES
DATE 05 07 79 08 22 75 10 09 75
GENUS
SPECIES
00
00
04
00
02
03
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE
AVERAGE DIVERSITY
NUMBER OF TAXA
NUMBER OF SMPLES COMPOBITKD
MAXIMUM DIVERSITY MAXH
MINIMUM DIVERSITY MINH
TOTAL DIVERSITY
TOTAL NUMBER Or INDIVIDUALS/ML
EVENNFSB COMPONENT
RELATIVE EVENNESS
MEAN NUMBER OF INDTVIDUALS/TAXA
NUMBER/ML OF MOST ABUNDANT TAXON
05 07 75 08 22 75 10 09 75
H
S
M
XH
NH
D
N
J
RJ
L
K
2.15
12.00
3.00
3. 58
0.09
3614.15
1681.00
o.6o
0.60
140.01
841.00
2.53
24.00
3.00
4.56
0.10
7293,99
2883.00
O.SS
0.55
120.13
920.00
2.51
27.00
3.00
4.75
0.32
2281.59
909.00
0.53
0,50
33.67
373.00
21
-------
LAKE HAKEI CHERRY CRBEK
STORE! NUMBER I 0104
TAX*
ANABAENA
ANABAtMUPSIS
ANKISTRODCSMUB fALCATO*
V. ACICULARIS
ANK18TRODE8MUS FALCATUft
V. MIRABIL18
A8TERIONELLA FORMOSA
RINUCLCARIA
CHROOMONA8 t
CLO8TERIUM
CLOITERIUN I]
CL08TERIUM II
COCCUNEI8 PUACrNTUlA
COELOSPHAERIUM PAtLIDUf
COSMAR.IUM
CRYPTOMDNA8 EROBA
CRYPTOMONA8 MAP880HII
CRYPTOMONA8 RC.FLEXA
CRYPYOMONA8 ROSTRATA
CYNATOPLEURA ELLIPTICA
CIMATOPLEUlt* SOLEA
CYMHELLA II
CYMBELtA MEXICANA
PIATOMA YENUE
T, ELON6ATUM
DIATOHK YULOARE
EPITHEMIA
EUGLENA OITURJd
V. MINOR
FLACELtATE
fRASILAKIA CROTONF.HBIB
OOMPHONEMA
OYRQIICMA
HAim»CHlA
CCLOtlMA ORANULAYA
». ANGU8T1SSIMA
MRLOSIRA ITALICA
KELUilIRA YARIAN1
DOUCEOTIA
DAVICUbA It
NAVICUbA 12
HAVICULA AMPHIROLA
KIYZICH1A
WIYESCHIA LONGISSIMA
Y. REVER8A
OEDOOONItlM
OOCYSYII
PEUTAITRUH DUPLEX
V. CbATHRATUM
PNACUS PLEURONECTE8
PHACUI YORYUB
RH01COSPHEN1A CURVATA
RHOPALODIA GIR8A
»L-SNEDt»MU» uUADRICAUDA
8TAURAITRUN
»YEPHA»ODI»CU1
•TEPHAHODI8CUS NIAGARAE
8YMEDRA ULNA
YRACHEIiUHONAg
YRACNELONONAS INTERMEDIA
TRACHELOMONA1 PUABCTOMJCA
TOTAfc
CONTINUED
05 07 75
0« 92 19
10 Of 71
FORM
PII.
PIL
Ctb
CEL
en
FIL
CEL
CEL
CEL
CEL
CCb
COL
CEL
CEL
CEL
CEL
CCL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CCL
CEL
CEL
CEL
CEL
ML
CEL
PIL
CEL
CEL
CEL
crt
CEL
TIL
COL
COL
CIL
CEL
CEl
CIt
COL
CIL
CEL
ML
CEL
ML
CEL
CEL
8
4
7
1
S
1
«c
Ifl
»«.*
90.0
18.7
J.'
1.*
I.I
».J
ALCAI.
IINIT8
PER ML
11
249
141
X
210
»1
X
11
1
11
lit
X
8
9
4
2
1
)
tc
«.J
*.4
4.1
a.t
31.9
11.*
2.1
10.6
8.4
AM1AL
UNITS
PER ML
X
X
121
X
X
X
114
111
«1
920
X
410
41
X
X
X
X
107
X
X
X
X
114
X
8
4
1
a
I
11
I
»c
u.«
11. •
11.*
4,»
4.1
4.<
4.1
141.0
1
AL0AL
UNIT8
PER ML
X
X
124
114
114
X
X
X
X
X
41
X
X
41
X
X
X
41
X
41
I 1T1
1 X
1M1
2111
•Of
22
-------
LAKE NAME! CUCHARAS RES.
STORET NUMBER! 0805
NYGAARD TROPHIC STATE INDICES
DATE 09 71 75
NYXUPHYCEAH 02/0 X
CHLOROPHYCEAN 06/0 E
EUGLENOPHYTE 0.13 7
DIATOM 0.31 E
COMPOUND 10/0 E
PALMER'S ORGANIC POLLUTION INDICES
DATE OB 91 73
OENU8 16
HBECIES 09
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 08 31 79
AVERAGE DIVERSITY H 1.73
NUMBER OF TAXA 8 15,00
NUMBER Of SAMPLES COMPOSITED M 1.00
MAXIMUM DIVERSITY MAXH 3.91
MINIMUM DIVERSITY NINH 0.00
TOTAL DIVERSITY D 137102.84
TOTAL NUMBER Of INDIVIDUALS/ML N 73897,00
FVENNrS* COMPONENT J 0.44
RELATIVE EVENNESS RJ 0.44
MEAN NUMBER Or INDIVIDUALB/TAXA L 4926.47
NUPKER/ML or HURT ABUNDANT TAXON K 42045.00
23
-------
LAKE HAKE i CUCHARM RM,
STORE? NUMBERI 080S
TAIA
ftCTIMASTRMN GR»CTLIMIIN
ANKIKTKODfSMUS TAUCATUS
V, ACICULARIS
CHROOMONAS 1
CKIPTOMONAS
CI»«OPH»TAII
CYCbOTELliA
DACTILOCOCCOPail
KVQbENA
HICRACTIIIIUM PIUILL'IM
NAVICULA
HITtSCKIA
HITMCHIA tONSISKIMA
V. dtVCRSK
OOCtlll*
8CEKCDESHU8 D1NDNPNUS
fCHCDMMUl OUADR1CAUDA
TOTAL
CaNHNUED
01 II 79
rORM
COL
cu
CtL
en
rib
CCb
eci/
CIL
COL
C£L
ML
CtL
CEb
COL
COL
«
1
1
4
2
IS
ALOAL
UNITS
%C PER ML
O.Ji 212
1
6.11 4672
0.9) 6J7
0.11 212
11.71 2I21S
4.0| 2171
54, »| 4204S
O.Jt 112
0,»» 4JS
I X
D.9I »)7
1
0.11 212
1 X
1 X
1 0.6| 428
71*47
24
-------
LAKE NAME| DILLON
8TURET NUMBERI 0806
NYCAARD TROPHIC STATE INDICES
DATE 08 28 75 10 19 78
MYXOPHYCEAN 0/0 0 01/0 E
CHLOROPHYCEAN 01/0 E 01/0 E
IUGLENOPHYTE 0/01 T 0/03 T
DIATOM 0.19 E 0/04 T
COMPOUND 02/0 [ 02/0 E
PALNl'RiS ORGANIC POLLUTION INDICES
DATE 08 25 73 10 19 75
6ENUS 00 00
SPECIFB 00 00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 08 98 79 10 19 75
AVERAGE DIVERSITY H 9.10 1.80
NUMBER Of TAXA 8 9.00 12.00
NUMBER Or BAHPLES COMPOSITED M 4.00 4.00
MAXIMUM DIVERSITY MAXH 1.17 3.58
MINIMUM DIVERSITY MINK 0.03 0.03
TOTAL DIVERSITY D 7606.20 10866,60
TOTAL NUMBER Or INDIVIDUALS/ML N 3692.00 6037.00
EVENNESS COMPONENT i) 0,66 0.50
RELATIVE EVENNESS RJ 0.66 0.80
MEAN NUMBER OF INDIVIDUALS/TAXA L 409.44 SOS.06
NUMBER/ML Or MOST ABUNDANT TAXON K 1537,00 3297.00
25
-------
IAKI NANCl DILLO*
1TOIIIT IUNIKRI 0101
TUA
AMUTIIODIMUS riLCATU*
A*T(RION[bLA roNKOl*
CHROOCOCCU* LIHMTICU*
eoceoio COLONY
C*Y»TONOIUI IMS*
cm
DINOIfttON
lUDORINA
rbJka«,utc it
m»au*»i» CMITONIIIIU
COKTMUCO
01 II 71
10 It II
ITALIC*
NOUatOTIk
OOCHTIf
•INCDR* CYCbOPUM
romi
»L
CCL
COL
COL
Clt
CIt
Ctl
cot
CCb
ctt
eel
cct
ra
Ctl.
CKL
en.
AbtAL
ON It*
• «C »M NL
1 1 I
ll»,7| 104
1 1
1 1
11*. 71 711
1 1
IIO.«| 114
1 1 X
142.41 l»17
110.41 114
1 1 I
1 1 I
1 1
1 1
1 1
1 1
I
%c
»4.»
1.1
4.»
I.I
t«.o
14. «
ALOAL
unit*
Kit HI
141*
I
(1
170
111
I
4*0
1J»7
X
X
X
X
1*11
•Oil
26
-------
LAKE NAMCl GRAND LAKE
3TIJRET NUMBER I 0807
NYGAARD TROPHIC STATE INDICES
DATE 08 26 75 10 10 75
MYXOPHYCEAN
CHLOROPHYCEAN
EUGLENOPHYTE
DIATOM
COMPOUND
1.50 C
0.50 T
0/04 I
1.00 E
a.so E
1.00 E
1.00 C
0/02 ?
0.50 C
3.00 E
PALMER'S ORGANIC POLLUTION INDICES
DATE 08 26 7S 10 10 75
GENUS
SPECIES
01
00
01
00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 08 26 75 10 10 75
AVERAGE DIVERSITY
NUMBER Or TAX*
NUMBER OF SAMPLES COMPOSITED
MAXIMUM DIVERSITY MAXH
MINIMUM DIVERSITY MIHH
TOTAL DIVERSITY
TOTAL NUMBER OF INDIVIDUALS/ML
EVENNESS COMPONENT
RELATIVE EVENNESS
MEAN NUMBER OF INDIVIDUALS/TAX*
NUMBER/ML Of MOST ABUNDANT TAXON
H
ft
M
XH
NH
D
N
J
RJ
L
K
1."
9.00
2.00
3.17
0.09
1695,07
1003.00
0.53
0.52
111.44
521.00
2,18
9,00
2,00
3.17
0,11
1702,58
781,00
0,69
0,68
86,78
339,00
27
-------
LAKE ftAHEl
-------
LAKE NAMEl GREEN MOUNTAIN
STORE! NUMBER I 0008
NYCAARD TROPHIC STATE INDICES
DATE 08 25 75 10 09 75
MYXOPHYCEAN 0/0 0 0/0 0
CHLOROPHYCEAN 0/0 0 0/0 0
EUGLENOPHYTE 0/0 ? 0/0 T
DIATOM 0.50 E 0.33 E
COMPOUND 01/0 E 01/0 E
PALMER'S ORGANIC POLLUTION INDICES
DATE 08 ?5 75 10 09 75
GENUS 01 01
SPECIES 00 00
SPCCIE6 DIVERSITY AND ABUNDANCE INDICM
DATE 06 95 75 10 09 75
AVERAGE DIVERSITY H n.59 1.11
NUMBER Or TAXA S ft.00 6.00
NUMBER Or SAMPLES COMPOSITED M 3.00 3,00
MAXIMUM DIVERSITY MAXH 2.51 2.58
MINIMUM DIVERSITY MINH 0.02 0.06
TOTAL DIVERSITY D 3354.98 1006.77
TOTAL NUMBEP OF INDIVIDUALS/ML N 3822.00 907.00
EVENNESS COMPONENT J 0.'23 0.43
RELATIVE EVENNE.88 RJ 0.23 0.42
MEAN NUMBER OP INDIVIDUALS/TAXA L 637.00 151.17
NUMBER/ML OF MOST ABUJiDANT TAXON K 3470.00 *35.00
29
-------
LAKE NAHEl CREEK MOUNT*IN RES.
8TORCT NUMBER I 0*08
TAXA
ASTER.TONELLA FORMOSA
CHROOMONAft 7
COMTINUrt)
01 3S TS
>0 0* 7S
CYCLOTCLLA COKTA
CYKBELLA
DINOBRYON
rOAGILAtllA CROTONEHSIB
QOKPHOKCMA
HANNABA ARCU8
TOTAL
FORK
CEL
CEL
CM.
CtL
CCL
CEL
en.
CEL
1
1
IS
111 1
1 1
141 1
III 1
1 1
1 1
lll«0
1 1
1 1
1C
.81
1
.11
.SI
1
.11
1
1
ALCAL
UNITS
PER ML
112
X
II
113
X
1470
1
1
IS 1C
1 1 1
1 1 1
111 ».»!
11170.01
1 1 1
1 1 1
1X11.41
1 I t
1 1 1
ALOAL
UNITS
PER ML
to
61S
X
112
X
X
JI92
• 07
30
-------
LAKE NAMEI HOLBROOK LAKE
STORET NUMBER! 0809
NYCAARD TROPHIC STATE INDICES
DATE OB 06 75 08 21 75 10 07 75
MYXOPHYCEAN
CHLOROPHYCEAN
EUGLENOPHYTE
DIATOM
COMPOUND
02/0 E
10/0 E
0.42 E
0.17 T
18/0 E
05/0 E
05/0 E
0.10 ?
2.00 E
13/0 E
S.OO E
5.00 E
0/iO ?
2.00 E
12.0 E
PALMER'S ORGANIC POLLUTION INDICES
DATE 05 06 75 08 21 73 10 07 75
GENUS1
SPECIES
20
07
12
00
08
00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE
AVERAGF. DIVERSITY
NUMBER OF TAXA
riUHBER OF tfArfE>LE8 COMPOSITED
MAXIMUM DIVERSITY MAXH
MINIMUM DIVERSITY MINH
TOTAL DIVERSITY
TOTAL NUMBER OP INDIVIDUALS/ML
EVENNESS COMPONENT
RELATIVE EVENNESS
NKAN NUMBER OF INOIVIDUAL8/TAXA
NUMBER/ML OF MOST ABUNDANT TAXON
05 06 75 08 21 75 10 07 75
H
S
M
XH
NH
D
N
J
RJ
L
K
2.00
27.00
1,00'
4.75
0.01
132836.00
66418,00
0.42
0.42
2459.93
36005.00
1.89
17.00
1.00
4.09
0.01
99444.34
52616.00
0.46
0.47
3095.06
33166.00
1.90
18.00
1.00
4.17
0.01
10*071.90
S6301.00
0.46
0.46
3117.83
30347.00
31
-------
LAKE NAME! HOLRHUOK LAKE
STORM NUMBERl 0809
TAXA
ACTIMA8TOUM GRACIblNim
ANABAENA
ANARAKNOP8I8 CIRCULARI8
ANA8AENnP8Id 8EHIAtA T
ANKISTRODESMUS FALCftTIIS
V. ACICULARI8
CHROOMOKM t
CLU8TERIUM
COEtiASTRUM HICROPOI-UM
COELASTRUM 8PHAERICUM
CPYPTUM()N»8 ECUS*
CRIPTONONA* MARR80NII
CILINDNI1THECA T 6KACILI8
DIATOHA TCNIIE
V. ELONOATUM
DICTIOSPHAERIUN PULCHELLIIM
CUOLENA
CUOLENA It
fLAGELLATC
GLENOniNIUM
GYRU8IGNA
NAV1CULA
NAVICULA CU8PIDATA
HITI8CH1A LONCI88IMA
V. REVER8A
OOCY8TI*
OCClltiDTDRIA II
OSCILLATOR!* I]
OICILbATORIA LIMNETICA
PCDIAVTRUN BORYANUM
PEDIABTMUN DUPLEX
V. RETICULITUM
PIIACU8 MCCALOP8I8
PHACU8 PSrUDONORDCTCDTII
•CENEOECMUf ACUHINATU*
•CEREDESMU8 DlNORPHUa
•CCNCDESMUS QUADR1CAHDA
•CENEOESMUf 8PP.
•TEPHANDDI8CU8
8TEPHANODI8CUS DUBIU8
SYHtDRA T
TETRASTRUN tLtCAMS
TETRAITRUN 8TAUROCENIAEFORME
TRACHILOHO»»8
TOTAL
CONTINUED
09 DC 79
08 It 78
10 07 78
PORN
COL
riL
ru
Ml
CEL
CIL
CEL
CEL
COL
CCb
CEL
CEb
CEL
COL
CEL
CCL
CCL
CEL
CEL
CCL
CCL
CCL
CCL
riL
FIL
ru
COL
COL
CCt
CCL
COL
COL
COL
COL
CCL
CCL
CCL
COL
COL
CCL
8
4
8
1
]
1
At.CH L
UNITS
%C PER Mb
X
2.0 1115
X
1.0 197}
X
0.7| 491
O.JI 1*4
1 X
0.51 119
t.II «OI)
0.91 }29
O.JI IM
1 X
1 X
1
I
1 X
1
1 X
S4.ll 1*005
1 X
1
1 X
0.7| 491
1 X
0.81 119
1 X
0.11 184
1
1
1.11 1117
14.81 1(440
|
1
1 X
a
i
i
5
1
4
%c
0.1
4.11
1.7
61.0
4.0
1.1
tt.O
4.9
2.9
o.«
0.1
ALSAL
UNITS
PER ML
1 181
1 1111
1 90S
11188
lilt
X
X
iioc
1441
18*1
X
X
X
tsoi
1 X
102
1 181
1
1
1
s
1
4
1
1
%c
!.»
SI. 9
1.1
0.9
0.1
11.1
ai.i
i.t
1.4
ALOAL
UNITS
PER ML
X
«9t
10147
X
X
X
109t
499
X
too
749T
11077
991
X
X
799
X
X
*«41I
aim
snot
32
-------
LAKE NANEl LAKE MEREDITH
BTORET NUMBER! 0810
NYGAARD TROPHIC STATE INDICES
DATE OS 06 75 08 31 75 10 07 75
MYXOPHYCEAN
CHLOROPHYCEAN
EUGLEMOPHYTE
DIATOM
COMPOUND
tu.0 E
4.00 E
0.14 T
0/05 ?
16.0 r
8.UV E
6.00 E
0.07 ?
0,33 E
16.0 E
08/0 E
03/0 E
0.09 7
0/03 T
12/0 E
PALMER'S ORGANIC POLLUTION INDICES
DATE OS 08 75 08 21 75 10 07 75
GENUS
SPECIES
20
00
11
06
08
00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE OS 06 75 08 21 75 10 07 75
AVERAGE DIVERSITY
NUMBER OF TAX*
NUMBER OF SAMPLES COMPOSITED
MAXIMUM DIVERSITY MAXH
MINIMUM niVERBITY MINH
TOTAL DIVERSITY
TOTAL NUHBFP OF IMPIVIDUALS/KL
EVENNESS COMPONENT
RELATIVE EVEKKESS
MEAN NUMBER OF INDIVIDUAL8/TAXA
NUMBER/ML OF MOST ABUNDANT TAXON
H
s
M
XH
NH
D
N
J
RJ
L
K
2.93
26.00
3.00
4.70
0.01
94741.55
32335.00
0.62
0.4J
1243.65
11340.00
1.82
21.00
3.00
4.52
o.on
315247.66
173213.00
0.40
0.41
7531.00
85012.00
0.22
15.00
3.00
3.91
0.00
209863.48
953934.00
0.06
0.06
63599.60
930950.00
33
-------
LAKE NANCl LAKr MtRKDkTH
STORE! NUMBERI 0110
TAX*
ANABAENA
ANABAENOP8I8 CIRCUHRIH
ANKIBTItODEBMUS
APHANOCAPSA
APHMIOTHECE T
CHLOROGOMUM
CHRDOCOCCUB
CHROOCOCCU8 DISPERSUS
COELOSPHAEMUH
COEL08PHAEMUN KUETIINGIAHUM
C08MADIUH
CRYPTOMOCAS MOSA
CYCLOTEtLA MENEUHINIANA
CYLINDROTHECA t CRACILI8
DACTYLOCOCCOP8IS
CNTOHOIir.18 ALATA
EUGLEN*
FLAGELLATE
FRACILARIA CROTONENBIS
GLENODIN1UM
GLENODIHIUM EOAX
GYROSIGMA
GYROBIGMA MACRUH 7
LAOERHEINIA
LfHOBTA
MERI8MOPEDIA MINIM*
KERI8MOPEDIA TCNUI8SINA
NICROCY8TI8 INCERTA
NATICULA
OOCT8TI8
08CIbI/ATDMIA It
OBCItiLATORIA 12
D8CILHTORIA LIMNETIC*
PHACU8
PHACU8 MEGALOP8I*
PliEUROIIGHA
8CENEOE8NU8 ACUNINkTUS
8CENEDEBHU8 OUACRICAUDA
8CHROEDHRIA 8ITIGERA
BPERMATOXOOP8I8
BPIRULIH*
TtTRAEDHOH MINIMUM
TETRA8TRUN BTAUROGENIAEfORMI
TOTAL
CONTIRUEO
OB 0* 73
01 11 7»
10 07 7S
rDRM
rit
rit
CEL
COL
COL
CEL
COL
COL
COL
COb
CCL
CEL
CCL
CEr.
CCL
CEb
CEL
CEL
CCL
CEL
CCL
CEL
CCL
CCL
riL
COL
COL
COb
CEL
CEL
PIL
FTL
riL
cct>
CRL
CCL
CCL
COb
CCL
CKb
CEL
ALOAL 1 ALGAL
UNITS | UHIT8
8 «C PEN ML 18 *C PER ML
1 1 131 6. (I 11477
41 3.71 B7I 141 2.0| 1400
1
t
)
a
cct i
COL 1
I
!.4I 17SC
It
X
X
X
20,41 H««
a, ii 7i2
0.21 71
J.4I 1171
10. H I4II
1 X
1.91 512
|
1
0.21 71
1
0.71 219
I
1 X
1
O.JI 146
a.ti *ti
10. »| 1411
!
IS. 11 11140
1
1.11 !»•
0.71 21»
1.4| 4J9
t X
1
1
1 X
1
1 X
s
1
2
1 X
1
t
1
t
1
0.91 1411
1
0.21 42f
1.01 1700
4.41 til
1 X
1
1
1 I
1
1 I
1
0.21 42S
1 X
1
0.21 425
1
0.21 421
4t.!l ISOU
l.tl 1*11
1
O.il ISO
1
0.11 111
It. II 4J7S9
1
1
1
1
0.11 211
1 X
0.31 ISO
1
1 X
1
ALGAL
UNITS
S %C PER ML
1 1
21 1.41 110*1
J
S
4
1
0.1
0.0
0.2
0.2
0.1
0.1
0.2
•7.4
O.I
O.I
11(4
2*1
201S
14SS
171
ll«4
X
X
14SS
tlOfSO
sia
sia
X
O.«| 291
1
12118
171211
9S1»J4
34
-------
LAKE NAME! MILTON RES.
STORET NUMBERl 0811
NYGAARD TROPHIC STATE INDICES
DATE OS 06 75 08 26 75 10 10 75
CHLOROPHYCEAH
EUGLENOPKYTC
DIATOM
COMPOUND
01/0 E
03/0 IE
0/04 T
0/01 7
04/0 E
02/0 E
02/0 E
0/04 J
0.37 E
07/0 E
1.00 I
6.00 E
0/07 7
0,50 I
9.00 E
PALMER'S ORGANIC POLLUTION INDICES
DATE 05 06 75 08 26 75 10 10 78
GENUS
SPECIES
04
04
01
00
04
02
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 05 Oft 75 08 26 75 10 10 75
AVERAGE DIVERSITY
NUMBER OF TAXA
NUMBER Or SAMPLES COMPOSITED
MAXIMUM DIVERSITY MAXH
MINIMUM DIVERSITY MINH
TOTAL DIVERSITY
TOTAL NUMBER Or INDIVIDUALS/ML
EVENNESS COMPONENT
RELATIVE EVENNESS
MEAN NUMBER Or INDIVIDUALS/TAX*
NUMBER/ML OF MOST ABUKDAHT TAXON
H
8
M
XH
NH
D
N
J
RJ
L
K
2, SO
8.00
2,00
3,00
0,17
1020,00
408.00
0,83
0.83
51.00
102,00
3.14
18.00
2.00
4.17
0.09
7878.26
2509.00
0.75
0.75
139.39
406.00
2.48
16.00
2.00
4.00
0.23
1798.00
725.00
0.62
0.60
45.31
272.00
35
-------
LAKE NAKTI NI1-TON «rB.
STORM NUMBERI 0911
T»XA
ANABAENA PLANCTONKA
APHANIZOMENON FLOS-AQUAE
ABTIRIONELLA FORMOSA
CHROOMONAB I
CLOSTCRIUM
CDCCONEIB PLACCNTUI,*
COELA8TRUM MICROPOHUN
CRTPTOMONAS
CRYPTOMONA8 ER08A,
CHTPTONONAS MARSSONII
CYANOPNYTAN FILAMENT
CYCLOTKLLA »(ENt:UHlllIAN«
CONTIIIUtO
09 0« 75
01 J» 7»
ID 10 75
FRAGILARIA 12
rRAOILARIA CROTOHF.NSie
MBLOSIRA nlKTANS
MELOBIRA 8RANULATA
V. ANOUSTH3IHA
NAVICULA II
NAVICULA It
NAVICULA II
NAVICULA 14
NITI8CHIA
OOCTBTIB
PCDIABTRUM BORTANUN
PEDIABTRUH SIMPLEX
V. DUODENAHIUM
PCHHATf DIATOM
BCENEDEBNUB OUAORICAVDA
8CHROEDEHIA
BCHRnEDERI* JIIDATI
RCHROEDERIA BETIGEP*
BPHAEROCTBTIB (ICHHOETtHl
BTEPHANODIKUK
TABELLAMIA FENeSTRATA
TOTAL
rn»«
TIL
FIL
CEL
CEL
Ctb
CEL
COL
CCL
CEL
CEL
riL
CEL
TEL
CEL
CEL
CEL
CCL
CEL
CCL
CEL
CCL
CIL
cot
COL
cob
eei,
COL
CCL
eei.
ctt.
cot
CCI.
CCL
ALGAL
UNITS
8 %C PER ML
4
5
a
t
i
3S.O
1J.S
U.5
JS.O
IJ.»
JJ.5
X
102
St
SI
X
102
SI
SI
ALOAL
UNITS
S 1C PER ML
1111.21 112
1 t
2114.11 1««
sm.ji 4ot
3
4
1
J.9I 74
4.4
10. J
11. t
11. •
11. t
l.»
S
111
X
X
X
as*
19J
2>S
1*1
X
X
X
T4
X
ALOAL
UNITB
B *C PC* ML
1 1
21 6.21 4S
1 1
4)17.91 272
1
8
1
(.2
12. i
IS.S
«.2
12.*
X
X
48
V!
X
X
lit
48
X
X
•i
X
X
X
40S
2SO«
728
36
-------
LAKE NAMEt NAVAJO LAKE
8TORET NUMBERI 0813
NY1AARD TROPHIC STATE INDICES
DATE OB 18 75 09 30 78
CHLOROPHiCEAN
DTATPM
COMPOUND
i.oo E
1.00 E
0/03 »
0/03 ?
3.00 E
oi/o &
0/0 0
0/01 I
0/0 T
01/0 E
PALMER'S ORGANIC POLLUTION INDICES
DATE 08 18 75 09 30 75
GENUS
SPECIES
00
00
00
00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 08 18 75 09 30 75
AVERAGE DIVERSITY H 0.73 1.1S
NUMBER OF TAXA S 8.00 S.OO
NUMBER OF SAMPLES COMPOSITED N 7.00 7.00
MAXIMUM DIVERSITY MAXH 3.00 3,33
MINIMUM DIVERSITY MINK 0.23 o.os
TOTAL DIVERSITY 0 337.53 1U2S.80
TOTAL NUMBEK OF INDIVIDUALS/ML N 316.00 693.00
EVENNESS COMPONENT J 0.34 O.BO
RELATIVE EVENNESS RJ 0.18 0.49
MEAN NUMBER OF INDIVIDUAL3/TAXA L 39.30 178.40
NUMBER/ML Of MOST ABUNDANT TAXON K 353.00 605.00
37
-------
LMCC imti M»»»JO in.tr.
8TORET WUMBBRI Otl?
TAXA
APHANIIOHINON FL08-ACUHE
CERATIUM HIRUNDINELLA
F. SCOT11CUM
CHROONONAS f
CRIPTOMONAI
CUtPTOMOHXS tR08>
CRtPIUMOM»« MHHSSONII
c»»t
COHTINUCD
01 1* 75
09 10 71
8PHABROCY8TIB aCHRC
-------
LAKE NAMEl SHADOW HTN. LAKE
STORE! NUMBER I 08J3
NYRAARD TROPHIC STATE INDICES
DATE OR 26 75 10 10 75
MYXOPHtrCEAi?
CHLOROPHYCEAN
DIATOM
COMPOUND
3.00 K
4.00 e
0/07 7
0.25 ?
8.00 E
i.oo e
3.00 E
0/04 ?
0.25 ?
6.00 e
PALMER*S ORGANIC POLLUTION INDICES
DATE OB 26 75 10 10 75
GENUS
SPECIES
05
00
01
00
SPECIES DIVERSITY AND ABUNDANCE INDICES
DATE 0« 26 75 10 10 75
AVERAGE DIVERSITY H 2,97 2.50
NUMBER Or TAXA S 15.00 22.00
NUMBER OF SAMPLES COMPOSITED M 3.00 3.00
MAXIMUM DIVERSITY MAXH 3.91 4,46
MINIMUM DIVERSITY MINH 0,07 0.14
TOTAL DIVERSITY D 7623.99 4452.50
TOTAL NIIMPKH Or INDIVIDUALS/ML N 2567.00 1781.00
EVENNESS COMPONENT J 0.76 O.S6
RELATIVE EVENNESS »<7 0.76 O.SS
MEAN NUMBER OF !NPTVin"ALS/TAXA L 171.13 80.95
NUMBER/ML OF MOST ABUNDANT TAXON K 630.00 706.00
39
-------
LAKE »A«I 8HAOON MTU. LAKE
STORET HUNKER I 0111
TAXA
ANABACIU
ANK1ITRQDESNUS rALCITU*
». ACICULARIS
APHAIIItONENOK FLOS-AQUAE
AlTtRIQNILLA rO«MO»«
CHROONONAS T
COINMIUN
CRYPTONCXAO
CRTPTDNONAO NARlSOiH
c*rpToito»4i» IPP.
CKLOTKLLA • ODAMCA
CYOt
OIATONA tCMUt
V. IbONCITUK
DICtIO»P«««RIU« PULCHCLLUN
DIKOIHIOK »»V»P1CI)»
OINOIRTOI OLINORICUM
II
CROfONm*I«
LCI>tU«Tkb*QN
OOKPNUIItlU
MtLLOMOItl
•OITOfllOU t IMITMI1
•CLOdM ITALIC*
NICKOCTITT* »CRUGI*OI«
•ITtKMIA
PCDUfTftUN »ORIAIIUM
PCOIklTRUM •IHrLEX
T, CUOD«»»RIUH
pri>«rC DtKTOM
rNORMtDIVM HUCICOLA
•Ct»IOMIIU» ARNATU1
fCHROIDKHIt (CTIOCRA
•PHAEROCT1T1S tCHROCTKRI
fPOROILOIIUM PLAKUM
ITCPHAMODtaCU*
ITNEDKA VLM
TAKbLANIA
TOTAb
0* It 71
10 10 IS
roM
rib
CEL
rib
CIL
Ctb
en
CCb
CCL
en
CtL
COL
CIL
COL
CM.
en
CtL
CCL
CtL
CtL
CtL
CtL
CtL
COL
CCL
COL
COL
CtL
rib
COL
CtL
COL
CCL
CtL
CCL
0
1
*
1
1
4
ALGAL
U II 1 11
%C PER XL
1
1
|
14.11 110
1.01 «7
11.11 201
1 I
1
1
1
1
1.01 40
1
I
1
1
1
I
1
I
1 X
1
1.01 40
1
11.11 HO
0.41 141
S.tl MS
1 X
1
10.01 *ll
1.01 41
|
J.OI 07
1
1
1
0
1
1
1
f
4
1C
1.7
11. 1
10.)
II. 0
1.7
10. »
10.1
o.t
AI.HAL
omo
PC* ML
X
II
14*
104
X
I
14*
X
X
X
X
X
X
11
X
X
700
104
X
III
X
X
J»47
1701
40
-------
TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO.
EPA-600/3-79-114
3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
DISTRIBUTION OF PHYTOPLANKTON IN COLORADO LAKES
5. REPORT DATE
December 1979
6. PERFORMING ORGANIZATION CODE
V.CTfo'rris, W.D. Taylor, L.R. Williams, S.C. Hern,
V.W. Lambou, and F.A, Morris
8. PERFORMING ORGANIZATION FIEPOBT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS . .
Environmental Monitoring ana Support Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Las Vegas, NV 89114
10. PROGRAM ELEMENT NO.
1BD884
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
U.S. Environmental T^rorlecTion Agency-Las Vegas, NV
Office of Research and Development
Environmental Monitoring and Support Laboratory
Las Vegas, NV 89114
13. TYPE OF REPORT AND PERIOD COVERED
02-21-75 to 12-11-75
14. SPONSORING AGENCY CODE
EPA/600/07
5. SUPPLEMENTARY NOTES
16. ABST
This is a data report presenting the species and abundance of phytoplankton
in the IJ lakes sampled by the National Eutrophication Survey in the State of
Colorada. Results from the calculation of several water quality indices are also
included (Nygaard s Trophic State Index, Palmer's Organic Pollution Index, and
species diversity and abundance indices).
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS
COSATI Field/Group
*aquatic microbiology
lakes
*phytoplankton
water quality
Colorado
lake eutrophication
Nygaard's trophic indices
Palmer's organic pollu-
tion indices
Species diversity and
abundance
06 C, M
08 H
13 B
B. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
19. SECURITY CLASS (This
UNCLASSIFIED
21. NO. OF PAGES
48
20. SECURITY CLASS (TTiiipage)
UNCLASSIFIED
22. PRICE
EPA Fo»«n 2220-1 {R,v. 4.77)
PREVIOUS EDITION IS OBSOLETE
*U-S.aOVER*IUeKT POINTING OFFICE: 1B7»—683-282/2219
------- |