United States
Environmental Protection
Agency
Environmental Monitoring
and Support Laboratory
P.O. Box 15027
Las Vegas NV89114
EPA-600/3-79-119
December 1979
Research and Development
Distribution  of
Phytoplankton in
Oregon Lakes

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                   RESEARCH  REPORTING SERIES

 Research reports  of the Office  of  Research and  Development, US Environmental
 Protection Agency, have been grouped into nine series. These nine broad categories
 were established to facilitate further development  and application of environmental
 technology.  Elimination of traditional grouping  was  consciously  planned  to foster
 technology transfer and maximum interface in related fields. The nine series are:


       1.  Environmental Health Effects Research
       2.  Environmental Protection Technology
       3.  Ecological Research
       4.  Environmental Monitoring
       5.  Socioeconomic Environmental Studies
       6.  Scientific and Technical  Assessment Reports (STAR)
       7.  Interagency Energy—Environment Research and Development
       8.  "Special" Reports
       9.  Miscellaneous Reports
 This report  has been assigned to the ECOLOGICAL RESEARCH series.  This series
 describes research on the effects of pollution on humans.plant and animal species, and
 materials.  Problems are assessed for their long-and short-term influences. Investiga-
 tions include  formations,  transport, and  pathway  studies  to determine the fate of
 pollutants and their effects. This work provided the technical basis for setting standards
 to minimize undesirable changes  in living organisms in the aquatic, terrestrial, and
 atmospheric environments.
This document is available to the public through the National Technical Information
Service, Springfield, Virginia  22161

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                                         EPA-600/3-79-119
                                         December  1979
 DISTRIBUTION OF  PHYTOPLANKTON IN OREGON LAKES

                       by

    W. D.  Taylor, L.  R.  Williams, S.  C.  Hern,
V. W. Lamhou, F.  A.  Morris*,   and M.  K.  Morris*

         Water and Land  Quality Branch
         Monitoring Operations Division
Environmental Monitoring and  Support  Laboratory
            Las Vegas, Nevada  89114
       *Department of Biological Sciences
        University of Nevada, Las Vegas
            Las Vegas, Nevada
ENVIRONMENTAL MONITORING AND SUPPORT  LABORATORY
       OFFICE OF RESEARCH AND DEVELOPMENT
       U.S. ENVIRONMENTAL PROTECTION AGENCY
            LAS VEGAS, NEVADA   89114

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                                DISCLAIMER

     This report has been reviewed by the Environmental  Monitoring and
Support Laboratory-Las Vegas, U.S. Environmental  Protection Agency, and
approved for publication.  Mention of trade names or commercial  products does
not constitute endorsement or recommendation for use.

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                                FOREWORD

     Protection of the environment requires effective regulatory  actions
which are based on sound technical and scientific  information.  This
information must include the quantitative description and  linking of
pollutant sources, transport mechanisms, interactions, and resulting  effects
on man and his environment.   Because of the complexities  involved,  assessment
of specific pollutants in the environment requires a total  systems  approach
which transcends the media of air, water, and land.  The  Environmental
Monitoring and Support Lahoratory-Las Vegas contributes to the  formation  and
enhancement of a sound monitoring data base for exposure  assessment through
programs designed to:

              •develop and optimize systems and strategies for  monitoring
               pollutants and their impact on the  environment

              •demonstrate new monitoring systems  and technologies  by
               applying them to fulfill special monitoring needs  of the
               Agency's operating programs

     This report presents the species and abundance of phytoplankton  in  the
8 lakes sampled by the National Eutrophication Survey in  the State  of
Oregon, along with results from the calculation of several commonly used
biological indices of water quality and community  structure.  These data  can
be used to biologically characterize the study lakes, and  as baseline data
for future investigations.  This report was written for use by  Federal,
State, and local governmental agencies concerned with water quality analysis,
monitoring, and/or regulation.  Private industry and individuals  similarly
involved with the biological aspects of water quality will find the document
useful.  For further information contact the Water and Land Quality Branch,
Monitoring Operations Division.
                                Director
            Environmental Monitoring and Support Laboratory
                               Las Vegas
                                    n

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                                CONTENTS

                                                                       Page

Foreword ...............................   lil

Introduction .............................     1

Materials and Methods  ........................     2

     Lake and Site Selection .....................     2

     Sample Preparation  .......................     2

     Examination ...........................     3

     Quality Control .........................     4

Results  ...............................     5

     Nygaard's Trophic State Indices .................     5

     Palmer's Organic Pollution Indices  ...............     5

     Species Diversity and Abundance Indices .............     7

     Species Occurrence and Abundance  ................     9

Literature Cited ...........................     10

Appendix A.  Phytoplankton Species list for the State
             of Oregon ........................     ^
Appendix B.  Summary of Phytoplankton Data ..............    13

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                                INTRODUCTION

     The collection and analysis of phytoplankton data were included in the
National Eutrophication Survey in an effort to determine relationships  between
algal characteristics and trophic status of individual lakes.

     During spring, summer, and fall of 1975,  the Survey sampled 156 lakes in
11 States.  Over 450 algal species and varieties were identified and
enumerated from the 430 water samples examined.

     This report presents the species and abundance of phytoplankton in the
8 lakes sampled in the State of Oregon (Table  1).  The Nygaard's Trophic
State (Nygaard 1949), Palmer's Organic Pollution (Palmer 1969),  and  species
diversity and abundance indices are also included.
          TABLE 1.  LAKES SAMPLED IN THE STATE  OF  OREGON
STORET No.
Lake Name
County
   4101


   4102

   4103


   4104


   4105

   4106


   4107

   4108
Brownlee Reservoir


Diamond

Hells Canyon
Reservoir

Hills Creek
Reservoir

Owyhee

Oxbow Reservoir


Suttle Lake

Waldo Lake
Baker (Washington
in  Idaho)

Douglas

Wai Iowa, Baker
(Adams in Idaho)

Lane
Maiheur

Baker (Adams in
Idaho)

Jefferson

Lane

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                            MATERIALS AND METHODS
LAKE AND SITE SELECTION

     Lakes and reservoirs included in the Survey were selected through
discussions with State water pollution agency personnel  and U.S.  Environmental
Protection Agency Regional  Offices (U.S. Environmental  Protection Agency
1975).  Screening and selection strongly emphasized lakes with actual  or
potential  accelerated eutrophication problems.  As a result, the  selection  was
1 imited to lakes:

     (1) impacted by one or more municipal  sewage treatment plant outfalls
         either directly into the lake or by discharge  to an inlet tributary
         within approximately 40 kilometers of the lake;

     (2) 40 hectares or larger in size; and

     (3) with a mean hydraulic retention time of at least 30 days.

Specific selection criteria were waived for some lakes  of particular State
interest.

     Sampling sites for a lake were selected based on available information on
lake morphometry, potential  major sources of nutrient input, and  on-site
judgment of the field limnologist (U.S. Environmental Protection  Agency 1975).
Primary sampling sites were chosen to reflect the deepest portion of each
major basin in a test lake.  Where many basins were present, selection was
guided by  nutrient source information on hand.  At each sampling  site, a
depth-integrated phytoplankton sample was taken.  Depth-integrated samples
were uniform mixtures of water from the surface to a depth of 15  feet
(4.6 meters) or from the surface to the lower limit of  the photic zone
representing 1 percent of the incident light, whichever was greater.  If the
depth at the sampling site was less than 15 feet (4.6 meters), the sample was
taken from just off the bottom to the surface.  Normally, a lake  was sampled
three times in 1 year, providing information on spring, summer, and fall
conditions .
SAMPLE PREPARATION

     To preserve the sample 4
(Prescott 1970) were added to
collection.  The samples were
Support Laboratory, Las Vegas,
                              milliliters (ml) of Acid-Lugol's solution
                              each 130-ml sample from each site at the time of
                              shipped to the Environmental Monitoring and
                               Nevada, where equal  volumes from each site

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were mixed to form two 130-ml  composite samples for a given lake.   One
composite sample was put into  storage and the other was used for the
examination.

     Prior to examination, the composite samples were concentrated  by the
settling method.  Solids were  allowed to settle for at least 24 hours prior to
siphoning off the supernate.  The volume of the removed supernate and the
volume of the remaining concentrate were measured and concentrations
determined.  A small (8-ml) library subsample of the concentrate was  then
taken.  The remaining concentrate was gently agitated to resuspend  the
plankton and poured into a capped, graduated test tube.  If a preliminary
examination of a sample indicated the need for a more concentrated  sample,  the
contents of the test tube were further concentrated by repeating the  settling
method.  Final concentrations  varied from 15 to 40 times the original.

     Permanent slides were prepared from concentrated samples after analysis
was complete.  A ring of clear Karo® corn syrup with phenol (a few crystals of
phenol were added to each 100  ml  of syrup) was placed on a glass slide.   A
drop of superconcentrate from the bottom of the test tube was placed in the
ring.  This solution was thoroughly mixed and topped with a coverglass.   After
the syrup at the edges of the  coverglass had hardened, the excess was scraped
away and the mount was sealed  with clear fingernail polish.  Permanent diatom
slides were prepared by drying sample material on a coverglass, heating  in  a
muffle furnace at 400° C for 45 minutes, and mounting in Hyrax®.  Finally,  the
mounts were sealed with clear  fingernail polish.

     Backup samples, library samples, permanent sample slides, and
Hyrax-mounted diatom slides are being stored and maintained at the
Environmental Monitoring and Support Laboratory-Las Vegas.


EXAMINATION

     The phytoplankton samples were examined with the aid of binocular
compound microscopes.  A preliminary examination was performed to precisely
identify and list all forms encountered.  The length of this examination
varied depending on the complexity of the sample.  An attempt was made to find
and identify all of the forms  present in each sample.  Often forms were
observed which could not be identified to species or to genus.  Abbreviated
descriptions were used to keep a record of these forms (e.g., lunate cell,
blue-green filament, Navicula #1).  Diatom slides were examined using a
standard light microscope.  If greater resolution was essential to accurately
identify the diatoms, a phase-contrast microscope was used.

     After the species list was compiled, phytoplankton were enumerated using
a Neubauer Counting Chamber with a 40X objective lens and a 10X ocular lens.
All forms within each field were counted.  The count was continued until a
minimum of 100 fields had been viewed, or until the dominant form had been
observed a minimum of 100 times.
®Registered trademark

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QUALITY CONTROL

     Project phycologists performed internal  quality control intercomparisons
regularly on 7 percent of the species identification and counts.  Although an
individual had primary responsibility for analyzing a sample, taxonomic
problems were discussed among the phycologists.

     Additional quality control  checks were performed on the Survey samples by
Dr. G. W. Prescott of the University of Montana at the rate of 5 percent.
Quality control checks were made on 75 percent of these samples to verify
species identifications while checks were made on the remaining 25 percent of
the samples to verify genus counts.  Presently, the agreement between quality
control checks for species identification and genus enumerations is
satisfactory.

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                                   RESULTS


     A phytoplankton species list for the State is  presented  in  Appendix  A.
Appendix B summarizes all  of the phytoplankton  data collected from  the  State
by the Survey.   The latter is organized by  lake,  and includes an alphabetical
phytoplankton species list with concentrations  for  individual  species given by
sampling date.   Results from the application  of several  indices  are presented
(Nygaard's Trophic State,  Palmer's Organic  Pollution, and  species diversity
and abundance).  Each lake has been assigned  a  four-digit  STORET number.
(STORET (STOrage and RETrieval) is the U.S. Environmental  Protection Agency's
computer system which processes and maintains water quality data.)   The first
two digits of the STORET number identify the  State; the last  two digits
identify the lake.


NYGAARD'S TROPHIC STATE INDICES

     Five indices devised  by Nygaard (1949) were proposed  under the assumption
that certain algal groups  are indicative of levels  of nutrient enrichment.
These indices were calculated in order to aid in determining the surveyed
lakes' trophic status.  As a general rule,  Cyanophyta, Euglenophyta, centric
diatoms, and members of the Chlorococcales are found in waters that are
eutrophic (rich in nutrients), while desmids  and many pennate diatoms
generally cannot tolerate high nutrient levels and  so are found in
oligotrophic waters  (poor in nutrients).

      In applying the indices to the Survey data, the number of taxa in  each
major group was determined from the species list for each sample.  The  ratios
of these groups give numerical values which can be used as a biological index
of water richness.   The five indices and the ranges of values established for
Danish lakes by Nygaard for each trophic state are presented in Table 2.   The
appropriate symbol,  (E) eutrophic and (0) oligotrophic, follows each
calculated value in  the tables in Appendix B.  A question mark  (?)  following  a
calculated value in  these tables was entered when that value was within the
range of both  classifications.


PALMER'S ORGANIC POLLUTION  INDICES

      Palmer  (1969) analyzed reports from 165 authors and developed algal
pollution indices  for  use in rating water  samples with high organic pollution.
Two lists of organic-pollution-tolerant  forms were prepared, one containing
20 genera, the other,  20  species  (Tables 3 and 4).   Each form was  assigned a
pollution index number ranging  from 1 for  moderately tolerant forms to 6 for

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  TABLE 2.  NYGAARD'S TROPHIC STATE INDICES ADAPTED FROM HUTCHINSON (1967)
Index
Calculation
Oligotrophlc
                Centric Diatoms + Euglenophyta
                        Desnrideae
Eutrophic
Myxophycean
Chlorophycean
Diatom
Euglenophyte
Compound
Myxophyceae
Desmideae
Chlorococcales
Desmideae
Centric Diatoms
Pennate Diatoms
Euglenophyta
Myxophyceae + Chlorococcales
Myxophyceae + Chlorococcales +
0.0-0.4
0.0-0.7
0.0-0.3
0.0-0.2
0.0-1.0
0.1-3.0
0.2-9.0
0.0-1.75
0.0-1.0
1.2-25
TABLE 3.  ALGAL GENUS POLLUTION INDEX
          (Palmer 1969)
                     TABLE 4.   ALGAL SPECIES POLLUTION
                               INDEX (Palmer 1969)
Genus
Anacystis
Ankistrodesmus
Chlamydomonas
Chlorella
Closterium
Cyclotella
Euglena
Gomphonema
Lepocinclis
Melosira
Micractinium
Navicula
Nitzschia
Oscillatoria
Pandorina
Phacus
Phormidium
Scenedesmus
Stigeoclonium
Synedra
Pollution
Index
1
2
4
3
1
1
5
1
1
1
1
3
3
5
1
2
1
4
2
2
Species
Ankistrodesmus falcatus
Arthrospira jenneri
Chlorella vulgaris
Cyclotella meneghiniana
Euglena gracilis
Euglena viridis
Gomphonema parvulum
Melosira varians
Navicula cryptocephala
Nitzschia acicularis
Nitzschia palea
Oscillatoria chlorina
Oscillatoria limosa
Oscillatoria princeps
Oscillatoria putrida
Oscillatoria tenuis
Pandorina morum
Scenedesmus quadricauda
Stigeoclonium tenue
Synedra ulna
Pollution
Index
3
2
2
2
1
6
1
2
1
1
5
2
4
1
1
4
3
4
3
3

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extremely tolerant forms.  Palmer based the index numbers on occurrence
records and/or where emphasized by the authors as being especially tolerant of
organic pollution.

      In analyzing a water sample, any of the 20 genera or species of algae
present in concentrations of 50 per milliliter or more are recorded.  The
pollution index numbers of the algae present are totaled, providing a genus
score and a species score.  Palmer determined that a score of 20 or more for
either index can be taken as evidence of high organic pollution, while a score
of 15 to 19 is taken as probable evidence of high organic pollution. Lower
figures suggest that the organic pollution of the sample is not high, that the
sample is not representative, or that some substance or factor interfering
with  algal persistence is present and active.


SPECIES DIVERSITY AND ABUNDANCE INDICES

      "Information content" of biological samples is being used commonly by
biologists as a measure of diversity.  Diversity in this connection means the
degree of uncertainty attached to the specific identity of any randomly
selected individual.  The greater the number of taxa and the more equal  their
proportions, the greater the uncertainty, and hence, the diversity (Pielou
1966).  There are several methods of measuring diversity, e.g., the formulas
given by Brillouin (1962) and Shannon and Weaver (1963).  The method which is
appropriate depends on the type of biological  sample on hand.

      Pielou (1966) classifies the types of biological samples and gives the
measure of diversity appropriate for each type.  The Survey phytoplankton
samples are what she classifies as larger samples (collections in Pielou's
terminology) from which random subsamples can be drawn.  According to Pielou,
the average diversity per individual  (H) for these types of samples can be
estimated from the Shannon-Wiener formula (Shannon and Weaver 1963):

                                S
                         H  =  -£ P. log  P.
where P is the proportion of the ith taxon in the sample, which is calculated
from n-j/N; n-j is the number of individuals per milliliter of the ith
taxon; N is the total number of individuals per ml; and S is the total  number
of taxa.  However, Basharin (1959) and Pielou (1966) have pointed out  that  H
calculated from the subsample is a biased estimator of the sample H, and  if
this bias is to be accounted for, we must know the total  number of taxa
present in the sample since the magnitude of this bias depends  on it.

     Pielou (1966) suggests that if the number of taxa in the subsample falls
only slightly short of the number in the larger sample, no appreciable error
will result in considering S, estimated from the subsample, as  being equal  to
the sample value.  Even though considerable effort was made to  find and
identify all taxa, the Survey samples undoubtedly contain a fair number of
rare phytoplankton taxa which were not encountered.

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      In  the Shannon-Wiener  formula,  an  increase  in  the  number of taxa and/or
 an  increase in  the  evenness of  the distribution  of  individuals among taxa will
 increase the average  diversity  per individual  from  its  minimal value of zero.
 Sager  and Hasler (1969)  found that the  richness  of  taxa was of minor
 importance in determination of  average  diversity per  individual for
 phytoplankton and they  concluded that phytoplankton taxa in excess of the 10
 to  15  most abundant ones  have little effect on H.   This was verified by our
 own  calculations.   Our  counts are in number per  milliliter and since
 logarithms to the base  2  were used in our calculations, H is expressed in
 units  of bits per individual.   When  individuals  of a  taxon were so rare that
 they were not counted,  a  value  of 1/130 per milliliter  or 0.008 per milliliter
 was  used in the calculations since at least one  individual of the taxon must
 have been present in  the  collection.

     A Survey sample  for  a  given lake represents  a composite of all
 phytoplankton collected at  different sampling sites on the lake during a given
 sampling period.  Since the  number of samples  (M) making up a composite is a
 function of both the  complexity of the  lake sampled and its size, it should
 affect the richness-of-taxa  component of the diversity of our phytoplankton
 collections.  The maximum diversity  (MaxH) (i.e., when the individuals are
 distributed among the taxa  as evenly as possible) was estimated from Iog2 S
 (Pielou  1966),  while  the minimum diversity (MinH), was estimated from the
 formula:


                MinH  =  -^-loq  1-  N - (s-])  log   N - 
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individual  should not be used  in direct comparisons  involving  various  samples
which have different numbers of taxa.   Since MaxH equals  log S,  the  expression
in sits is equal  to 1095 $>  or 1-  Therefore diversity in sits  per
individual  is numerically equivalent to J,  the  evenness component for  the
Shannon-Wiener formula.
SPECIES OCCURRENCE AND ABUNDANCE

  The alphabetic phytoplankton species list for each  lake,  presented  in
Appendix B, gives the concentrations of individual  species  by sampling date.
Concentrations are in cells, colonies, or filaments (CEL, COL,  FIL) per
milliliter.  An "X" after a species name indicates  that  the species identified
in the preliminary examination was in such a low concentration  that it did  not
appear in the count.  A blank space indicates that  the organism was not  found
in the sample collected on that date.  Column S is  used  to  designate  the
examiner's subjective opinion of the five dominant  taxa  in  a sample,  based
upon relative size and concentration of the organism.   The  percent  column  (%C)
presents, by abundance, the percentage composition  of each  taxon.

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                              LITERATURE CITED
Basharin, G. P.  1959.  On a statistical estimate for the entropy  of a
     sequence of independent random variables, pp. 333-336.  J_n: Theory of
     Probability and  Its Applications (translation of "Teoriya Veroyatnosei i
     ee Premeneniya").  N. Artin (ed).  4.  Society for Industrial and
     Applied Mathematics, Philadelphia.

Brillouin, L.  1962.  Science and Information Theory (2nd ed.).  Academic
     Press, New York.  351 pp.

Hutchinson, G. E.  1967.  A Treatise on Limnology.  II.  Introduction to Lake
     Biology and the Limnoplankton.   John Wiley and Sons, Inc., New York.
     1,115 pp.

Nygaard, G.  1949.   Hydrobiological  studies of some Danish ponds and lakes.
     II.  (K danske Vidensk. Selsk.)  Biol. Sci. 7:293.

Palmer, C. M.  1969.  A composite rating of algae tolerating organic
     pollution.  J. Phycol.  5:78-82.

Pielou, E. C.  1966.  The measurement of diversity in different types of
     biological collections.  J. Theor. Biol.  13:131-144.

Prescott, G. W.  1970.  How to Know the Freshwater Algae.  William C. Brown
     Company, Dubuque.  348 pp.

Sager, P. E., and A. D. Hasler.  1969.  Species diversity in lacustrine
     phytoplankton.  I.  The components of the index of diversity
     from Shannon's formula.  Amer.  Natur.  103(929):51-59.

Shannon, C. E., and W. Weaver.  1963.   The Mathematical Theory of Commu-
     nication.  University of Illinois Press, Urbana.   117 pp.

U.S. Environmental  Protection Agency.   1975.  National  Eutrophication Survey
     Methods 1973-1976.  Working Paper No. 175.  Environmental Monitoring and
     Support Laboratory, Las Vegas,  Nevada, and Corvallis Environmental
     Research Laboratory, Corvallis, Oregon.  91 pp.

Wilhm, V. L., and T. C. Dorris.  1968.  Biological parameters for water
     quality criteria.  Bio-Science.  18:477.

Zand, S. M.  1976.   Indexes associated with information theory in water
     quality.  J. Water Pollut. Contr. Fed.  48(8)-.2026-2031.
                                      10

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                   APPENDIX A



PHYTOPLANKTON SPECIES LIST FOR THE STATE OF OREGON
                       11

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Achnanthes exigua
Anabaena planctonica
Ankistrod.esmus faloatus
Arikistrodesmus falcatus
   v. acicularis
Ankistrodesmus falcatus
   V. mirabilis
Aphanizamenan flos-aquae
Astericnella formosa
Ceratium hinmdinella
Ceratium nirundinella
   f. piburgense
Ceratium h-Lrundinella
   f. ssottiaum
Ch t amijd cm anas
Closteridiwi
Closteriwn
Coccaneis
Coelastrwn m-iaroponMi
Coeloaphaeriim naecielianum
Cryptomanas erosa
Cryptomonas ovata
Cyclotella glomerata
Cymatopleura sdtea
Cymbella minuta
Dactyloooasopsis irregular>is
Diatcma vulgare
Uinobryon sooiale
Epifhemia sorex
Epithemia turv/ida
Euglena
Eunotia
F ragilaria capucina
   v. mesolepta
Fragilaria crotonensis
Glenodiniwn gymnodinium
Glenodinium oculatum
Gomphonema asuminatum
Gomphcnema olivaceum
Gamphanema parvulim
Gymnorliniijm fuscwn
Melosira granulata
Melozi-ra granulata
   v. cnvjustiss-ima
Melosira it alia a
Me I os i ra v a rims
Microaystis aeruginosa
Navi'cula ouspidata
Naviaula tripunetata
Navicula tripunotata
   v. sc.hisonemoid.es
Nitzschia aaic.ularis
Nitzschia apisulata
Nitzschia vermiaularis
Ooeystis
Pantf. orina morum
Pediastnm boryanim
Pediastrwn duplex
    V. clathratim
Peridinium inconspiauim
PeriAinium wnbonatum
Phorfm'dium mueicola
Rhoiaosphenia curvata
Ssenedesmus  acwninatus
Scenedesmus  bijuga
Scenedesmus  quadriaaud.a
Sahroederia  setigera
Skeletonema  potamos
Sphaerocystis schroeteri
Staurastrum
Stephanodiscus astraea
    v. minutula
Stephanod.iscus dubius
Stephanodiscus niagarae
Surirella ovata
Synedra ulna
 Tabellaria fenestrata
Tetraedron minimum
 Trachel omanas
                                    12

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                                    e to
                                   in
                 APPENDIX B.  SUMMARY OF PHYTOPLANKTON DATA


     This appendix was generated by computer.   Because it was only possibl
use upper case letters in the printout, all  scientific names are printed  i
upper case and are not italicized.

     The alphabetic phytoplankton lists include taxa without species  names
(e.g., EUNOTIA, EUNOTIA #1,  FLAGELLATE, FLAGELLATES, MICROCYSTIS INCERTA?,
CHLOROPHYTAN COCCQID CELLED  COLONY).   When species determinations were  not
possible, symbols or descriptive phrases were  used to separate taxa for
enumeration purposes.   Each  name on a list,  however, represents a unique
species different from any other name on the same list, unless otherwise
noted, for counting purposes.

     Numbers were used to separate  unidentified species of the same genus.   A
generic name listed alone is also a unique species.   A question mark  (';))  is
placed immediately after the portion  of a name which was assigned with
uncertainty.  Numbered, questioned, or otherwise designated taxa were
established on a lake-by-lake basis;  therefore NAVICULA #2 from lake  A  cannot
be compared to NAVICULA#2 from lake  B.  Plural ized  categories (e.g.,
FLAGELLATES, CENTRIC DIATOMS, SPP.) were used  for counting purposes when  taxa
could not be properly differentiated  on the  counting chamber.
13

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LAKE W.IMEl SRDhNLEF RF'S.
8TORET NUMBEPI 4101
                                              NYGAARD TROPHIC STATE INDICES

                                        DATE   04 09 75  08 04 75  09 15 75
  XYXOrHYCEAN
CHLOROPHYCEAN
 EUGLENOPHYTE
       DIATOM
     COMPOUND
                                                0/0  0
                                                02/0 E
                                                o.so e
                                                o.n ?
                                                05/0 E
           3.00 e
           1.00 E
           0/04 ?
           3.00 E
           7.00 E
01/0 E
0/0  0
0/01 ?
2.00 E
03/0 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   04 09 75  08 04 75  09 15 75
                                       GENUS
                                     SPECIES
     04
     00
                               01
                               00
    01
    00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVERAGE DIVERSITY
                         ifiwpfp or TAXA
           NUMBER OF AAMPLEK COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                      MIMMUM DIVEPSITY MINH
                        TOTAL DIVERSITY
         TOTAL NUMBER OF INDIVIDUALS/ML
                     F.V&NNE&5 COMPONENT
                      RELATIVE EVENNESS
        MEAN NUMBER OF IMDIVIDUALS/TAXA
       NUMbEH/ML OF MIST ABUNDANT TAXUN
04 09 75  06 04 75  09 IS 75
H
S
M
XH
NH
D
N
J
HJ
L
K
0.86
10.00
5.00
4.25
0.02
9568.36
11126.00
0.20
0.20
585,58
9749.00
1.02
12.00
5.00
3.58
O.OB
1681.98
1649.00
0.28
0.27
137.42
1312.00
1.22
7.00
5.00
2.81
0.03
3766.14
3087.00
0.43
0.43
441.00
2254.00
                                          14

-------
LAKE NAME! BROWNLEf  RES.
RTOBET NljMBrpi  4101
                                    CONTINUE!)
                                                                    01 04  75
                                                                                      09
                                                                                            75
TAXA

ANKI8TRODESMU8 t'ALCATUS
APHANIIOMEHON rLOS-AOUAE
ASTKRIONfLLA FORMOSA
CERATIUM HIRUNDINELLA
 r. P1BUHGI.KM
CHROOMONAS » ACUTA
COCCONKIS
CHVPTOMONAB
CYHATOPLEURA BOLEA
UIATOHA VULGARE
EUGI.ENA
FRAOILARIA CROTONKNS IK
(JLEKOUINIUH UlfMNlMIINIlIM
COHPHUNEWA II
RIIMPHUNrMA 12
MEI.UBIRA CRANllbATA
MELOSIRA 1TAL.ICA
HICBOCYSIIS AICRUGINOSA
NAVICULA TJUPHNCTATA
NITISCHIA VEHHtCULARIS
PHURNIDIUM HUCICOL.".
8CKNEDC8MUS ACUMINATU8
SCHRCIPEHIA SETIGERA
KCHROEDERIA HETIGERA
OKfLETONEHA PUTAMOS
STAURASTRUH
               AHTRACA
 V. HINUTULA
RUBIREUA CJYATA
niNCDRA ULNA
TARELLARIA rrNEITRATA
ZOOSPOHI

  TOTAL


IORM
CEti
rit,
CEL
CEL
CEL
CtL
CEL
crt,
CEL
CEL
CEL
CVl
CEL
CEL
CEL
ceu
COL
CEL
CEL
TIL
COL
CEL
CEL
CEL
CEL
CfL
CEL
crt
CEL
CEI.
CCL



1


2











9


4








1
1






»C


5.4

».J


O.J


0.6
0.1


0,«


1.8


0.3





17.6
'.II
1
0.*


ALGAL
UNITS
PER ML
>

«0)

>4
X

34
X
X
«7
14
X
X
101


161
X

M





9749
93S
X
67

11196


5




4

J



1



J



















%C




•>.!

2.2



7«.l>



9.1

















ALQAL
UNITS
PER ML

X

X
HO

17



1319



HO

X


X


X
X
X
X





1644


a

3












1
2

5



4












»C

10,1












73.0
14.6

1.1



t.l










ALGAL
UNITS
PER ML

312


X









3354
4S1

3S



35








X 1
3017
                                                15

-------
LAKE NAMEl DIAMOND
STORET NUMBER I 4102
                                              NYGAARD TROPHIC STATE INDICES

                                        PATE   07 16 75  10 31 75

                                 MYXOPHYCEAN    01/0 E    1.00 E
                               CHLOROPHYCEAN    0/0  0    2.00 E
                                EUOLENQPHYTE    0/01 ?    0/03 ?
                                      DIATOM    0.80 E    0.09 ?
                                    COMPOUND    04/0 E    4.00 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   07 16 75  10 31  75
                                       GENUS
                                     SPECIES
     00
     00
01
00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVERAGE DIVERSITY
                         NUMBER OF  TAXA
           NUMBER Q? SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                      MINIMUM DIVERSITY MIMH
                        TUTAL DIVERSITY
         TOTAL NUMBER OF INDIVIDUALS/ML
                     EVENNESS COMPONENT
                      RELATIVE EVENNESS
        MEAN NUMBER nr INDIVIDUALS/TAX*
       NUMBER/ML OP MOST ABUNDANT TAXON
1)7 16 75  10 31 75
H
S
M
XH
NH
D
N
J
RJ
L
K
0.22
in. 00
u.oo
3.32
0.05
47B.50
2175.00
0.07
0.06
217,50
2113.00
1.71
20.00
2.00
4.32
0,25
1456.42
852,00
0.40
0.36
42.60
402,00
                                        16

-------
LAKE NAMIr DIAMOND
STORE! NUHBEHI 410}
TAXA

ACHNANTHM EXIGUA
ANABAENA
ASTtRIONrLLA FORMOSA
CHAOOMUNAR J
CHRdONONAS } ACUTA
COCCONEIB
CRtPTOMONIR
CYCLOTELLA
CYCLOTELLA GLOHrRATA
CYMBELLA
EPITHEMIA
EPITHEMIA TURGID*
rn»i:n,ARlA CACUCINA
 V. MESOLCPTA
FRACILARIA CROTOHEN8I8
GnKPHUNEMA ACUMINATUM
HCLOIIRA
NAVICULA II
NtVICULA 12
NAVICULA CUSPIDATA
PANDORINA HORUM
PKNMATE DIATOM
RIIOICOBPHtNIA CURVATA
scENEncsfiua OU«DRIC*UD»
RCHROEDENIA SKTICER*
8TAURABTRUM
                                   CONTINUED
                                                  07
                                                        75
                                                                   10 Jl
  TOTAL
fORK
CEL
rii
CEL
CEL
CEL
CCL
CEL
CEL
CEL
CEL
CEL
CCL
CKL
CEL
CEL
CEL
CEL
CEL
CEL
COL
CEL
CEL
COL
CEL
CEL
CFl
CEt

•

>
1
1
























%C
I
1.4
1*7.1
1.4
























ALGAL
UNITS
PER Mb

11
3)13
31



X

X

X

X

X


X







X
2175
8

5






2
1
4














t


«C

2.8


5.5



)l.l
2.1
3.8














47.1


ALGAL
UNITS
PER ML
X
34


47
X
X

111
24
14

X
X
X

X
X

X
X
X
X
X
X
403

IS]
                                             17

-------
LAKE NAMEl HELLS CANYON PE6.
STOPET NUMBER! 4103
                                              NYGAARD TROPHIC STATE INDICES

                                        DATE   04 08 75  OB 04 75  09 15 7S

                                 MYXOPHYCEAN    0/0  0    01/0 B    1.00 C
                               CHLOROPHYCEAN    03/0 E    03/0 E    J.OO E
                                FUGLrNOPHYTE    1.00 E    0/OJ 7    0/04 T
                                      DIATOM    0.23 7    1,50 E    0,50 E
                                    COMPOUND    07/0 C    06/0 E    7,00 C
                                            PAI.MER«8 ORGANIC POLLUTION INDICES

                                        DATE   04 08 75  09 04 75  09 15 75
                                       GI.'NUS
                                     SPECIES
     06
     no
01
oo
01
oo
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVERAGE DIVERSITY
                         NUMBER OF TAXA
           NUMBER OF SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY HAXH
                      MINIMUM DIVERSITY HINH
                        TOTAL DIVERSITY
         TOTAL NUMBER Or INDIVIDUALS/ML
                     EVENNESS COMPONENT
                      RELATIVE EVENNESS
        MEAN NUMBER OP INDIVIDUALS/TAXA
       NUMBER/ML OF MOST ABUNDANT TAXON
04 08 75  08 04 75  09 15 75
H
S
M
iXH
:NH
D
N
J
RJ
L
K
0,90
74,00
3,00
4.58
0.04
7497,90
R331.00
0,30
0.19
347.13
7332.00
1,54
9.00
3.00
3.17
0.01
17845.53
11588,00
0,49
0,49
1287,56
6938,00
1.40
19.00
3.00
4.25
0.14
3133.80
1517.00
0.33
0.31
79,84
795.00
                                          18

-------
LAKE NAMEl HELLS CUNYMN Pr».
ttBHft NUMBCRl 4101
CONTINUKD
                                                   04  01  75
                                                                    01  04  IS
                                                                                           7»
T»K»

AHABAMA
AIIKIBTRODE8MUB MLCATUS
APHHIIIZONENON fLOS-AQUAD
A»TCRtONBLLA fOHHCJSA
CtRATIUN HIRUHDINeLLA
CHHOOMONJIS t
ClOBTERIDIUN
coccontia
CRtPTOMONAS [ROSA
CRIPTONONAS 0»ATA
CfMBELLI II
CTMELLA 12
CIMBELLA MINUTA
DIATOM* VULGARE
D1NOBRTON SOCIAL!
PRACILARIA II
FRAGILARIA CROTONENJIS
OOHPHONRMA OLIVACEUM
MCL08IRA GRANULATA
MtLOllRA VJPHHS
NAVICULA II
NAVICULA 19
NITZ1CHIA II
NlTtlCHIA ACICULAHIB
lITlaCHIA APICULATA
PtDtAITRUM DDPHX
 V. CLATHRATUK
PCRID1NIUM
•CENCDCRNUa
BCHHUCDERIA SCTldCNA
4HRIIEDER1A (EtlCERA
SKKLCTONEHA PUTAMOS
•PHAIPOCYSTIB ICHROCTCRI
HTAURAftTRIIN
HTEPHANUDIBCUg
BTEPHAHODIKCUS II
STCPHANnDISCUl DIIBIUg
STCPHAHOPISCU1 (PP.
SURIRCLLA OVATA
STNr.DRA
TRACHCbQMuNAR

  TOTAL
rORM
fit
CEti
ru
CEL
CEL
CEL
CEt
CEt
CEL
CEt
CCL
CEL
CEl
cct
CEL
CEL
CEL
CCL
CCL
CCL
CEL
CEL
CEL
cr.L
CEL
CEL

COL
CEL
COL
CEL
CEL
CEL
COL
CCL
CEL
CCL
CEL
CEl
CEL
CEL
CEL
ALGAL
UNITS
B «C PER ML



a













i

s


















i
4


1
0.4| )7
1
l,*l ]««
1
1
1
1 X
O.»l 74
1
1 K
1 X
I
1
0.41 J7
0.9| 74
1 I
t.Jl 111
1 I
l.tt 141
|
1 X
1 X
1 X
0.41 17
1 X
1
1
0.4| 17
1 X
1
1
|
1
1
1
1 >
1 X
11.01 7111
l.H 111
I
0.41 37
ALSAL
UNITS
B %C PER PL
1
1
41 1.1
)| 5.3




S








1

?


























1.4








5».l

38.7












1.9


0.4








122
601




!«]








• 9JI

1121










X

40J
X

41






ALGAL |
UNIT!
• %C PER ML





2



4









1











S



1











53.4



2.4









40, S











2.4



2,4






X


^
<
7»»
I
X
X
]«


X
X



X

U4
X






X



1«

X
X
16




X

                                                     •111

-------
LAKt: NAME|  HILLC CREEK PCS.
PTflPET NUMBER I  4104
                                              NYfiAAPD TROPHIC STATE INDICES

                                        DATE   03 28 75  07 16 75  10 30 75
                                 HYXOPHYCBAN
                               CHLOPOPHYCEAN
                                EUGLENOPHYTE
                                      DIATOM
                                    COMPOUND
 0/0  0
 0/0  0
 0/0  ?
 0/0  T
 0/0  0
0/0  0
01/0 E
0/01 ?
0/03 ?
01/0 E
0/0  0
0/0  0
0/0  ?
1.00 E
02/0 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   03 28 75  07  16 75  10 30 75
                                       GENUS
                                     SPECIES
     00
     00
    00
    00
    05
    00
                                         SPECIES  DIVERSITY  AND  ABUNDANCE INDICES
                                        DATE

                      AVERAGE  DIVERSITY     H
                         MUMBEP  OF  TAXA     8
           NUMBER OF  SAMPLES COMPOSITED     M
                      MAXIMUM  DIVERSITY  MAXH
                      MINIMUM  DIVERSITY  MINH
                        TOTAL  DIVERSITY     D
         TOTAL  NUMBER OF INDIVIDUALS/ML     N
                     FVEMKESS  COMPONENT     J
                      RELATIVE EVENNESS    RJ
        MEAN  NUMBER OF INDIVIDUALS/TAX*     L
       NUMBER/ML  OF MOST ABUNDANT TAXON     K
03 28 75  07 16 75  10 30 79
0.37
2.00
2.00
1.00
0.04
87.32
236.00
0.37
0.35
118.00
219.00
1.16
7.00
2.00
2.81
0.04
2459.20
2120,00
0.41
0.41
302.86
1569.00
0.89
7.00
2.00
2.81
0.02
4258.65
4785,00
0.32
0.32
683.57
4118.00
                                          20

-------
LAKC lUNCt HILLS CXlt*. KtS.
*TORCT NUMBCRl 4104
                                   COMlNUtO
                                                  03 21 75
                                                                   07
                                                                         7g
                                                                                     10 30 79
T»XA

•«KISTRODr8«U8 MLCATUA
AITIRItmeitA fORHOSA
CMTRIC DIAtOH
CCRATIUM HlBUNnlNILH I. SCOTTtCUM
CHLANYDOWQNAI
CHKOQMUIIAS i HCUTH
CRTPTONONtR

           CROTONrNRIH

MCL08JB* 5R»HUL«T»
 V. (KCUST1SS1M*

  TOTAL


roM
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CKL
CEL
CCL

CCL
ALGAL
UNITS
i »e »M HI,





\
2










1?.t
1.2










11*
17




,
i
18
"FT™
IJHO
i i
i t
i i
141 2
12114
I 1
11174
1 1
1 1


%C
1
.01
1
1
1
.01
.0)
1
.01
1
I
ALGAL
UNITS
PER ML
X
,
I
II
1 1


%C
1
112 121 4.71
191 2.31
X
1
1
Ml 2.11
42
297

1349
X

1
|
|
13
1
1
1.91
1
I
3.11
1
1
i i i mis. ii
ALGAL
UNITS
prp. MI

223
lit

111
74

X
14*


4111
                                                      236
                                                                      2120
                                                                                       47IS
                                               21

-------
LAKE NAMEt OWYHEC
STORET NUMBER! 4105
                                              NYGAARD TROPHIC STATE INDICES

                                        DATE   04 08 75  06 Ot 75  09 16 75
                                 MYXOPHYCEAN
                               CHLOROPHYCEAN
                                EUCLENOPHYTE
                                      DIATOM
                                    COMPOUND
01/0 E
01/0 E
0.50 E
0.50 E
05/0 I
04/0 E
02/0 E
0.17 T
1.00 E
09/0 E
01/0 E
0/0 0
0/01 T
2.00 E
09/0 G
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   04 OB 75  08 01 75  09 16 75
                                       GENUS
                                     SPECIES
06
00
05
00
00
00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVERAGE DIVERSITY
                         NUMBER OF TAXA
           NUMBEK OF SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                      MINIMUM DIVERSITY MINH
                        TOTAL DIVERSITY
         TOTAL NUMBER OF INDIVIDUALS/ML
                     OVENNE8R COMPONENT
                      RELATIVE EVENNESS
        MEAN NUMBER OF INOIVIDUAL8/TAXA
       NUMBER/ML OF MOST ABUNDANT TAXON
                                               04 08 75  08 01 75  09 16 75
H
A
M
XH
NH
I)
N
J
RJ
L
K
7.40
1J.OO
4.00
J.70
0.20
1569. 60
654.00
0,«5
O.O
50. Jl
207.00
2.08
13.00
4.00
1.70
0.18
1474.72
709.00
0.56
0.54
54.54
304.00
0.92
8.00
4.00
1.00
0.12
563.96
613.00
O.J1
0.28
76.63
409.00
                                            22

-------
LAKE lUHEl OWtHEr
STOUT NUNtCRl 4108
T»XA

ANAIACNA
APHANltUMENON rLOS-HOUAC
CE»ATIUH HmUNtllHeui.A
CHLAMYDOKONA*
CHKOOMOHAS t ACUTA
COEMJSPHASMUK N»ECIL1»NIIM
CRYPTOMOm*
CRIPTOMONAS CROSA
ClfCLOTELLA
CU61ENA
rHASILARU CROtONCNStS
OLCNODIN1UM It
CLENODINIUN 12
GOMPHDNCMk
GOMPHONCMt PARVULUM
MCLOS1RA fll*«NULATA
XtLOSlRA GPANUIATA
 1.  ANGUSTlaatMA
NtTICULA
NITZSCH1A
PCN1ATC DIATOM
PCRIOIIIIUM UMIONATUM
PHOHMIOIUH
PHORMIDIUH MIICICOLA
»«OICOSPHF,m» CURYATA
neeNepciiMus IIJUSA
8CHROEDCRIA SETICCRA
•TEPHANODISCUS
TRACHELOMONAB

   TOTAL
                                   CONTINUED
                                                  04  01  75
                                                                    01  01  75
                                                                                     04
                                                                                           71
JOB"
rn
TIL
CCL
CCL
CCL
COL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
CCL
Clt
TIL
TIL
CIL
COL
CCL
CEL
eci.
ALGAL 1 ULCAl
UNITS 1 UNITS
S «C PCD ML 1* 1C FED HL



4
1


1
3












9









10. t
11.)


9.1
JI.7


».l


9.1






10.6






II 1 X
13114.11 101
I
69 1
JOT I
II
1
14 tl
307 |
|
1
14 1
1
|
14 1
1
X 1
> 1
1
X 1
1
«9 1
14
X 1
H
X 1
1
X 1



43.9

11.4














14.3

1.3






S04

1S1
X
X
X





X

X

X

101

SI
X


ALQAL
'It lit
s »e pen ML
1 1
1U6.7I 409


1

























31.1























X

104

X





X
X

X










X

                                                23

-------
LAKE NAME! OXBOW RES.
STORE! NUMBER! 4106
                                              NYGAARD TROPHIC STATE INDICES

                                        DATE   04 09 75  08 04 75  09 15 75
                                 MYXOPHYCEAN
                               CHLOROPHYCEAN
                                EUGLENOPHYTE
                                      DIATOM
                                    COMPOUND
 0/0  0
 03/0 E
 0.33 E.
 0,37 E
 07/0 e
01/0 E
0/0  0
0/01 ?
0.50 E
04/0 E
          0/02 0
          2.00 E
          0/04 ?
          0.67 E
          3.00 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATE   04 09 75  08 04 75  09 15 75
                                       GENUS
                                     SPECIES
     01
     00
    00
    00
              02
              00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVEPAGC  DIVERSITY    H
                         fjiiHhtjR  OP TAXA    s
           NUMBtR  OF  SAMPLES COMPOSITED    M
                      MAXIMUM  DIVERSITY MAXH
                      MINIMUM  DIVERSITY MINH
                        TOTAL  DIVERSITY    D
         TOTAL  NUMBEK OF INDIVIDUALS/ML    N
                     EVENNESS  COMPONENT    J
                      RfcJ.ATTVE EVENNESS   RJ
        MEAN  NUMBER OF I NO! VI DUALS/TAX A    L
       NUMBER/ML t)F MOST ABUNDANT TAXON    K
04 09 75  08 04 75  09 15 75
   0.69
  17.00
   3.00
   4.09
   0.04
4351.83
6307.00
   0,17
   0.17
 371.00
5621.00
  0.36
 11.00
  3.00
  3.46
  0.22
167.04
464.00
  0.10
  0.05
 42.18
433.00
            0.62
           12.00
            3.00
            3.58
            0.14
          565.44
          912.00
            0.17
            0.14
           76.00
          807.00
                                          24

-------
LAKE HAMEl 0180" RES.
STORET NUHRCDt 4106
CONTINUED
                                                  04 09  7S
                                                                    01  04  7S
                                                                                     09  IS  IS
TAXA
 T. ACICULARIS
Amtl*TRODE«MUB IALCATUK
 V. K1RARILII
APHANIZOMENON fLO
ASTCRIONELtA FORMOSA
CHMOOKONH8 7
CLntTtRIUM
CUELAM'RUM MICROPPRUM
rRVPT(IMmi»R
CPTPTO»ON>0 ERORA
CICLOTtbLA
EUtitEMA
FRAC11.ARIA
rPkCILADIA CRDTDNENStS
CLKNOU1KIUM OCULATUK
Mrl.O61R» CRAKULtTA
"ELOBIPA ITALICA
MELDIIPA VARIAMR
NATICULA
NATICULA TRIPUNCTATA
 V. fCHUUNEMDICLCB
NAVICVLA TRIPUNCTATA
 ». (CHIZPUEMDIDES
KITltCHIA
NITtgCMIk ViRHICULARIS
OOCTITI*
PfFIAtTRUM BOP.OANUM
PIUIAfTRUM DUPLtl
 ». ClATHRATUN
KHtllCUIPHCNIA CURVAT*
IPHAEIinCtllTIS ICHPOETERI
»TE»H»NOD18CU8
BUItlRELLA
8THEDPA ULNA
TtKtUARIA rENriTRAl'A

  TOTAL


FORM
CCL
CEL
rn
CCL
cr.L
CCL
CEL
COL
CEL
CEL
CCL
CCL
CEL
CEL
CEL
CEL
CEL
CEL
CCL
CCL
CCL
CCL
CEL
CCL
COL
COL
CCL
COL
CEL
CCL
CEL
CIL
CEL


S



1
9











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1
4




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4.1
0.7






0.7




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0.7


ALGAL
UNITS
PER ML
X
X

100
4]

X



X
4)

X
X

257


X

X
X

X




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41

X


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i















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ALGAL
UNIT*
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X



X
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I

X
I

X





I


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X




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a




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1.1
7.7




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ALGAL
UN1TI
PER ML





X

g

IB
70


X

107


X




X

X

X
X


X

                                                     (107
                                                                                        111
                                             25

-------
LAKE MMEl BUTTLE LAKE
STORE! NUMBER I  4107
                                              NYGAARD TROPHIC STATE INDICES


                                        DATE   03 28 75  07 16 75  10 31 75
                                 MYXOPHYCEAN
                               CHIOKOPHYCEAN

                                EUGLENOPHYTE
                                      DIATOM
                                    COMPOUND
 02/0 E
 0/0  0
 0/02 ?
 0,75 E
 05/0 E
01/0 E
0/0  0
0/01 1
0.67 E
03/0 E
0/01 0
J.OO E
0/03 7
0.40 E
5.00 E
                                            PALMER'S ORGANIC POLLUTION INDICES


                                        DATE   03 28 75  07 16 75  10 31 75
                                       GENUS
                                     SPECIES
     01
     00
    00
    00
    00
    00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVFPAGF DIVERSITY
                         NUMBER OF TAXA
           NUMBER OF SAMPLES COMPOSITED
                      MAXIMUM DIVERSITY MAXH
                      XINIMUK DIVERSITY MINH
                        TOTAL DIVERSITY
         TOTAL NUMBER OF INDIVIDUALS/ML
                     £VE»NF:86 COMPONENT
                      RELATIVE: EVENNESS
        MEAN NUMBER OF UDIVJDUAL8/1AXA
       NUMBER/ML OP MOST ABUNDANT TAXON
03 28 75  07 16 75  10 31 7S
H
S
M
XH
NH
D
N
J
RJ
L
K
0.62
11.00
1 .00
3.46
0.08
970.30
1565.00
0.1P
0.16
142.27
1374.00
0.35
7.00
1.00
2,81
0.05
473.55
1353.00
0.12
0.11
193.29
1261.00
2.31
17.00
1.00
4.09
0.11
3917,76
1696.00
0.56
0.56
99.76
637.00
                                          26

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LAKE CANT I IDTTLt LAKE
8TCIP.IT NUMBER I 4107
                                   CONTINUtD
                                                   0)
                                                         79
                                                                    07 16 7»
                                                                                     111 31 7F
ANABAEKA PLANCTONICA
ANABKM
1NA.BEKA PLANCTOHICA
AKTERIOkELLA FORMOSA
CtRATlUM HIRUNDINF.LLJ
CH1.OROPKTTAN FILAKFtil
CHROOKOKAS T
CPCCOMIS
CRVPTOMONA* EROBA
CTHBEI.LA
DACTYLPCOCCOPSIS  IRHtGUtARIB
           TURCIDA
           CRUTONENSIB
GLrNDDINIIIM  DCULATt'H
GTMNODIN1UM  rUSCUH
Mrl.DSJKA  GPANUL»IA
MClQCtKk  ITALICA
DOCYIT1B
8PHAEROCYSTI8 SCHROtTCRI
8T»URA8TRUH
STKPHANOni«CU8 NIAGAPAE
ITNEDRA
8INEDPA  ULHA
TKTRAEDRON MINIMUM

   TOTAL
rORM
FIL
FIL
ru
ru
Ctl
Ctl
FIL
CtL
cr.i
cri
Ctl
CtL
Ctl
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
CEL
COL
CEL
CEL
CEL
CEL
CEL
AlCAL
UK1T8
8 »C tlK ML











J







1





a













».»







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l.«


X










183

X
X


X
X
IJ74
X



X
J«
X

ALGAL
UNITS
8 »C PER ML
1 1
1193.11 1J6J


3

























6.7

























90
X









X




I



X
%


ALGAL
UNIT*
a %c PEP. ML




s


4

3





2








1
1
1 X
1 X
2.11 J«
1
2.11 IS
It. 71 VII
2.11 3S
».2! 10*





29. 1






2.1

1117. »
|
|
X

X


49!>
X



X
X
IV
X
«37


1 I. II IS
                                                      ists
                                                                       1JS1
                                              27

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LAKE NAPEl WALDO LAPP
STIIHET NUMBERS 410H
                                              NYCAARD TKOPHIC STATE INDICF8

                                        DATE   07 16 75  10 31 75
                                 NYXOPHYCEAN
                               CHLOROPHYCEAN
                                KUGLENOPHYTE
                                      DIATOM
                                    COMPOUND
 0/0  0
 0/0  P
 0/0  ?
 0.20 ?
 01/0 E
0/0  0
0/0  0
0/0  ?
c.so F:
01/0 E
                                            PALMER'S ORGANIC POLLUTION INDICES

                                        DATK   07 16 75  10 Jl 75
                                       GENUS
                                                    00
                                                    00
               00
               00
                                         SPECIES DIVERSITY AND ABUNDANCE INDICES
                                        DATE

                      AVERAGE DIVERSITY    H
                         NUMBER OF TAXA    S
           NUMBER OF SAMPLES COMPOSITED    M
                      MAXIMUM DIVERSITY MAXH
                      MINIMUM DIVERSITY MINH
                        TOTAL DIVERSITY    D
         TOTAL KUKDPR OF INDIVIDIJALS/KL    N
                     EVCKNESS COMPONENT    J
                      RELATIVE EVENNESS   RJ
        MEAN NUMBER nr INOIVIDUALS/TAXA    L
       NUMBER/ML or MOST ABUNDANT TAXON    K
07 16 75  10 31 75
   1.49
   7.00
   2.00
   2.fll
   1.78
  25.33
  17.00
   U.5J
  -0.28
   2.43
   9.00
  1.81
  4.00
  2.00
  2.00
  0,59
 57.92
 32,00
  0.91
  0,87
  0.00
 12.00
                                          28

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LAKE KANEi »ALDO LtRf
IITQRET HUHBERI 4108
TAXA
ASTER IOMEt,L»
CINBEI/bA
rimoTiA
GbENUOINlUM OCUlATim
NeLOBlRA ITALIC*
NAYICULA CUSPIUATA
PEMIDINIUM INCOHSPICHUM
aTEPHANODIKCUS
                                   CORTIHUED
                                                  01
                                                                    10 Jl  7?
1
1
TORN IS
CIL 11153
CIL 1
CEL 1
CKL 1
CEL 1
CHL 1
CEL 13111
CEL 1 1
CEL 11133
ALUIL
UNITS
»C VCR Ml,
.»! V
X
X
X
.«! 4
1 X
.'1 4
1
1
IS
1113'
1 1
1 1
14111
nm
i i
i i
i i
Din
»c
.si
i
.51
.SI
1
1
1
.SI
ALflAL
UMTS
PtR KL
13
4
11
4
                                                  29

-------
                                    TECHNICAL REPORT DATA
                             (Please read Instructions on the reverse before completing)
1. REPORT NO.
     EPA-600/3-79-119
                                                             3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE

  DISTRIBUTION OF  PHYTOPLANKTON IN OREGON LAKES
              5. REEORT DATE
                 December  1979
                                                             6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
  W.D.  Taylor, L.R.  Williams, S.C. Hern,  V.W.  Lambou,
  F.A.  Morris, and M.K.  Morris
                                                             8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
  Environmental Monitoring and Support  Laboratory
  Office of Research  and Development
  U.S.  Environmental  Protection Agency
  Las Vegas, NV   89114
              10. PROGRAM ELEMENT NO.

               1BD884
              11. CONTRACT/GRANT NO.
 12. SPONSORING AGENCY NAME AND ADpRESS
  U.S.  Environmental  Protection Agency-Las Vegas, NV
  Office of Research  and Development
  Environmental Monitoring  and Support  Laboratory
  Las Vegas, NV   89114
                                   1OD COVERED
              14. SPONSORING AGENCY CODE
               EPA/600/07
15. SUPPLEMENTARY NOTES
16. ABSTRACT
       This is a  data  report presentina; the species and  abundance of phytoplankton
  in the 8 lakes  sampled by the National  Eutrophication  Survey in the State of
  Oregon.  Results  from the calculation of several water quality indices  are also
  included (Nygaard's  Trophic State  Index, Palmer's Organic Pollution Index, and
  species diversity and abundance  indices).
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
  *aquatic microbiology
   lakes
  *phytoplankton
   water quality
b.IDENTIFIERS/OPEN ENDED TERMS
Oregon
lake eutrophication
Nygaard's  trophic indices
Palmer's organic pollu-
tion indices
Species diversity and
abundance
                                                                           c.  COSATl Field/Group
06 C, M
08 H
13 B
 8. DISTRIBUTION STATEMENT
  RELEASE TO PUBLIC
                                               19. SECURITY CLASS (This Report I

                                               UNCLASSIFIED	
                            21. NO. OF PAGES
                              36
                                               20. SECURITY CLASS (This page)
                                               UNCLASSIFIED
                                                                           22. PRICE
EPA Form 2220-1 (Rev. 4-77)    PREVIOUS EDITION is OBSOLETE
                                                             »U.S. GOVERNMENT PRINTING OFFICE: 1979— 68 J-282/2214

-------