SEPA
           United States
           Environnn"
           Age1
           Industrial Environmental Research F
           Laboratory
           Cincinnati OH 45268
Research and Development
Control  of Odors
From Anaerobic
Lagoons Treating
Food  Processing
Wastewaters

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                RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology. Elimination of traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:

      1.  Environmental Health  Effects Research
      2.  Environmental Protection Technology
      3.  Ecological Research
      4.  Environmental Monitoring
      5.  Socioeconomic Environmental Studies
      6.  Scientific and Technical Assessment Reports (STAR)
      7.  Interagency Energy-Environment Research and Development
      8.  "Special" Reports
      9.  Miscellaneous Reports

This report has  been assigned  to the ENVIRONMENTAL PROTECTION TECH-
NOLOGY series. This series describes research performed to develop and dem-
onstrate instrumentation, equipment, and methodology to repair or prevent en-
vironmental degradation from point and non-point sources of pollution. This work
provides the new or improved technology required for the control and treatment
of pollution sources to meet environmental quality standards.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                      EPA-600/2-78-151
                                      July 1978
   CONTROL OF ODORS FROM ANAEROBIC LAGOONS
    TREATING FOOD PROCESSING WASTEWATERS
                     by

              J. Ronald Miner
     Agricultural Engineering Department
           Oregon State University
           Corvallis, Oregon 97331
          Contract No. CC691935-J
              Project Officer

             Jack L. Witherow
      Food and Wood Products Branch
Industrial Environmental Research Laboratory
            Corvallis, Oregon 97330
INDUSTRIAL ENVIRONMENTAL RESEARCH LABORATORY
     OFFICE OF RESEARCH AND DEVELOPMENT
    U.S.  ENVIRONMENTAL PROTECTION AGENCY
           CINCINNATI, OHIO 45268

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                           DISCLAIMER
This report has been reviewed by the Industrial Environmental Re-
search Laboratory - Cincinnati, U. S. Environmental Protection
Agency, and approved for publication.  Approval does not signify
that the contents necessarily reflect the views and policies of
the U. S. Environmental Protection Agency, nor does mention of
trade names or commercial products constitute endorsement or rec-
ommendation for use.

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                                  FOREWORD

     When energy and material resources are extracted, processed, converted,
and used, the related pollutional impacts on our environment and even on our
health often require that new and increasingly more efficient pollution con-
trol methods be used.  The Industrial Envuronmental Research Laboratory-
Cincinnati (lERL-Ci) assists in developing and demonstrating new and improved
methodologies that will meet these ends both efficiently and economically.

     This report reviews the physical chemistry involved in odor release
from liquid surfaces, mechanisms of odor perception, and techniques currently
employed for odor measurement.  These concepts and data are applied to anaer-
obic lagoons as presently being used to produce a low cost, energy-efficient
device for treatment of organic wastes.  Field experiences are related in
which meat packing plant lagoons presented a problem due to odor complaints.

     Finally, the technology is identified which must be developed in order
for anaerobic lagoons to receive societal sanction.  A research plan is pre-
sented to evaluate one proposed solution to the technology need.  For further
information on the subject, contact H. Kirk Willard, Chief, Food and Wood
Products Branch.

                              David G. Stephan
                                  Director
                Industrial Environmental Research Laboratory
                                 Cincinnati

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                                  ABSTRACT
      Anaerobic lagoons are used for the treatment of meat packing  wastes
in most areas of the country.  They are a relatively low cost means of achiev-
ing BOD reduction.  Although lagoon effluent is not suitable for stream dis-
charge, it is amenable to further treatment or to land application.

      One of the most serious limitations of anaerobic lagoons in this appli-
cation is odor production.  Odor complaints have been widespread but have
been most frequent in areas of high sulfate waters and during start-up.
There has been little specific research effort devoted to anaerobic lagoon
odor control.

      This report assembles existing information relative to odor control
associated with anaerobic lagoons used in the meat packing industry and ident-
ifies opportunities for productive research.   It provides a basis for
approaching the overall problem in a comprehensive fashion.  This report
identifies six research techniques which could make significant contributions
toward solving the odor problems.

      This report was submitted in fulfillment of Contract No. CC691935-J by
the Agricultural Research Foundation of Oregon State University under the
sponsorship of the U.S. Environmental  Protection Agency.   It covers the
period July 1, 1976 to December 31, 1976, and work was completed as of Dec-
ember 31, 1976.
                                     IV

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                            CONTENTS


Foreword	 iii

Abstract	  iv

Figures and Tables	  vi

Acknowledgments	 vii


   1.   Introduction	   1

   2.   Doctrine of Nuisance	   3

   3.   Theory of Odor Perception	   5

   4 .   Quantitative Measurement of Odors	  15

   5.   Release of Ammonia and Hydrogen
       Sulfide from Liquid Surfaces	  20

   6.   Anaerobic Lagoon Design and
       Operation	  29

   7.   Field Experience	  31

   8.   Problem Analysis	  36

   9.   Research Needs	  39


References	  40

Appendix	  43
                                v

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                             FIGURES


Number                                                   Page

  1     Scentome ter	 17

  2     Effect of solution pH on fraction
        dissolved reactant dissociated (ionized)	 26





                             TABLES


Number                                                   Page

  1     The human senses, examples of parameters
        measured and alternate information sources	  6

  2     Basic odor quality classifications as
        described by five authors	 11

  3     Dilution to threshold values with various ports
        open on a scentometer when an odor is barely
        detectable	 18

  4     Results of laboratory anaerobic lagoon
        studies to predict sulfide concentrations	 27

  5     Analysis of a gas sample collected in a bell
        jar over an anaerobic meat packing waste
        lagoon in New Zealand	 32

  6     Composition of gases inside the Controliner at
        University of Illinois swine farm	 35
                              VI

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                               ACKNOWLEDGMENT
      The preparation of this report was supported in part by Contract
CC691935-J, U.S. Environmental  Protection Agency.   The counsel  and coopera-
tion of Jack L. Witherow, Project Officer, Food and Wood Products Branch,
Industrial Environmental Research Laboratory-Ci, Corvallis, Oregon, is
gratefully acknowledged.
                                    Vll

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                            SECTION 1

                          INTRODUCTION
Anaerobic lagoons have been identified as low-cost means of
treating meat plant wastes.  They have achieved widespread appli-
cation throughout this country and much of the world.   The great-
est application has been to small- and medium-sized packers;  how-
ever, anaerobic lagoons have been utilized for several larger
packing plants.

Anaerobic lagoons are basically engineering earthen basins which
detain waste while it undergoes anaerobic bacterial decomposition.
The rate of decomposition is a function of organic matter concen-
tration and temperature.  Since meat packing wastes typically in-
clude significant proportions of hot water, they respond well to
lagoon treatment.

One of the most frequent problems mentioned relative to anaerobic
lagoons is odor.  Being an anaerobic biological process, the end
products are highly reduced components.  Typical constituents are
ammonia, methane, hydrogen sulfide, and carbon dioxide.  Other
intermediates involved in the decomposition of many by-products
include amines, mercaptans, alcohols, ketones, and other more
complex organic compounds.  Therefore, anaerobic lagoon contents
are a solution of input, intermediate breakdown products, and end
products of bacterial decomposition.

In order for an odor problem from an anaerobic lagoon to arise,
a series of events must occur.  The odorous compound must be
discharged to the lagoon or be formed within the lagoon by an-
aerobic biochemical processes.  The odorous compound must vola-
tilize and escape from the lagoon surface.  The volatile odorous
gas must be transported from the lagoon surface to the proximity
of people.  And finally, there must be people to serve as re-
ceptors who are offended by the smell they perceive.

The chain of events necessary for an odor problem to arise also
provides a key to the control of odors.  Remedial measures which
halt or modify any one or more of the events leading to odor pro-
blems can be expected to decrease or change perceived odor in-
tensities.

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The purpose of this report was to assemble existing information
concerning anaerobic meat packing plant waste treatment lagoon
odors in a single location and to draw from this information
guidance in effective odor control technology development.

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                            SECTION 2

                      DOCTRINE OF NUISANCE


Ownership of land includes the right to impregnate the air with
odors, dust and smoke, pollute the water, and make noise, pro-
vided these actions do not substantially interfere with the com-
fort of others or hamper the use and enjoyment of their property.
Whenever a person uses his land in such a way as to violate this
principle, he may be guilty of maintaining a nuisance.  Thus, the
doctrine of nuisance acts as a restriction and is applied to a
series of wrongs which may arise from unreasonable, unwarranted
or unlawful use of property to produce annoyance, inconvenience,
discomfort or hurt that the law will presume to be a damage.  What
constitutes a nuisance in a particular case must be decided based
upon the facts and circumstances of that instance.

Nuisance has been classified as either public or private.  A
nuisance is said to be public when the public at large or some
considerable portion of it is affected or when the act is done in
violation of law.  When a public nuisance is involved, legal action
may be brought by a public official.  A private nuisance generally
affects only one person or a specific number of persons and is
grounds for a civil proceedings only.

The fact that a business is carried on carefully and in accord-
ance with the ordinary methods employed in that business does not
relieve the owner or person responsible for liability to a. neigh-
bor if that business is unreasonable and constitutes a nuisance.
Paulson  (1967) states that a livestock feeding operation, in itself
lawful, is not a nuisance per se.  When it interferes with another's
use and enjoyment of property or injures that property, it may
become a nuisance by virtue of the way it is maintained or operated.
The precise degree of discomfort that must be produced to constitute
a nuisance must be determined upon the basis of being reasonable
or unreasonable.

For an odor to be considered a nuisance, the stench must be of-
fensive to the senses and materially interfere with the comfortable
enjoyment of property within the area  (Paulson, 1967).  It is not
necessary that the odor be harmful or unwholesome.  It is suffi-
cient if it is offensive or produces such consequences, inconven-
ience or discomfort as to impair the comfortable enjoyment of pro-
perty by persons of ordinary sensibility.

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A person who suffers damages or feels that he suffers damages be-
cause of the odor of a livestock operation has two courses of ac-
tion open to him: a suit for damages, or a suit to enjoin or abate
the nuisance—and he may pursue either or both.  The remedies of
injunction or abatement are generally considered harsh by the
courts; normally, only that part of the operation which amounts
to a nuisance will be abated or enjoined.  When the nuisance re-
sults solely because of the method of operation or manner in which
business is conducted, the decree will be formed only to prevent
that particular method or manner rather than prohibiting completely
the use of the property by the person creating the nuisance.
It is an essential element for injunctive relief that annoyance
or injury be continuous or recurrent.  The use of premises which
create a nuisance may be enjoined, however, if it reasonably ap-
pears that such annoyances or injury will be recurrent.

To be liable for actual damages one need only create or commit a
nuisance.  The injured party is normally allowed to indicate the
best or most advantageous use of his property; this is then used
as a basis for determining the amount which would be adequate and
fair compensation (Paulson, 1967) .  Punitive damages are allowed
only when it can be shown that one has created and persistently
maintained a nuisance with reckless disregard for the rights of
others, or when one has reason to believe that his act may injure
another and does it in defiance of the rights of others.  The mere
commission of an act justifying an award of actual damages is not
sufficient to justify the award of punitive damages as a penalty.

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                           SECTION 3

                   THEORY OF ODOR PERCEPTION
Each of the five senses is used to bring us information about our
environment.  The ability to touch, taste, hear, see and smell
brings us into contact with our external world in such a way as
to give us a better understanding of its status and changes.  The
response to these senses is essentially a personal reaction.
Whether a sound is pleasant, a feeling satisfying, a taste enjoy-
able, a scene beautiful, or a smell desirable is fundamentally
an individualized response based upon cultural background and
momentary disposition of the individual.

The senses of touch, taste, hearing and sight all measure environ-
mental parameters also measurable by other techniques as shown
in Table 1.  The sense of smell is unique in that no mechanical
or chemical alternative device exists for measuring odor.  Thus,
odor is largely a subjective phenomenon for which no quantitative
standard of comparison exists.

The human nose is the basic detector in odor analysis.  It may
be supplemented in some cases by other instruments, but there is
no replacement for it even though the complexities of its function-
ing are not thoroughly understood  (Earth, 1970).  Moulton (1965)
stated that the final approach in odor and flavor identification
is "a bioassay based on stimulation of the human nose.  But the
behavioral response of a man is not a simple objective index of
olfactory sensitivity.  It is the end product of a complex flow
of interacting events, molded by the needs and experiences of the
individual—by the input of many classes of information.  Yet, in
the last analysis, there is no adequate substitute."  Measurement
of odor intensity and quality must be preceded by an understanding
of the way the nose functions to distinguish one odor from thousands
of others.

Inspired air typically travels into the nostrils, through the tur-
binate area and down the throat to the lungs.  Normal breathing
causes only a small portion of the air to reach the olfactory cleft
and the olfactory nerves, located high in the nasal cavity.   A
"sniff," characterized by a short burst of air inspired at a rate
greater than normal breathing, brings about extensive turbulence
and diffusion to all parts of the cavity.  Odor sensation is much
more obvious from a concerted sniff than from the normal breathing
rate.

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TABLE 1.  THE HUMAN SENSES, EXAMPLES OF PARAMETERS MEASURED
            AND ALTERNATE INFORMATION SOURCES

Sense
Sight
Sound
Taste
Touch
Smell
Parameter
measured
Color,
intensity
Pitch,
intensity
Saltiness,
sour
Temperature ,
hardness
Odor
Alternate
device
Colorimeter,
light meter
Tuning fork,
microphones
Chemical
titration pH
Thermometer
None
Unit of
measurement
Wave length,
lumens
Frequency,
decibels
mg/1, pH unit
Degree
None

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The olfactory nerve cells are located in the olfactory cleft area.
The total olfactory reception area in the adult human is about
two inches square.  Olfactory cells are long and narrow, and are
oriented perpendicular to the surface of the receptor area; cells
are pigmented yellow or yellow-brown.  There are different kinds
of odor receptor cells—cells that respond to different odor stim-
uli.  On the exposed end of the nerve cells are five to eight ol-
factory hairs extending into or through the mucous layer which
coats the surface of the mucous membrane.

It is believed that olfactory hairs  are the means of reception
of the "signal" of the odorous molecule.  A chain of events fol-
lows which instantaneously gives odor perception.  The hairs are
kept moist by the mucous layer.  An excess of mucus, as in the case
of a head cold, can incapacitate the hairs and, thus, the sense
of smell.

Moncrieff (1966) describes the mechanism of olfaction in six stages:

     (a)   The molecules of a volatile substance are continually
          lost to the atmosphere.
     (b)   Some of the molecules, inspired with air into the nasal
          cavity, are directed to the olfactory receptors.  The air
          of a sniff is beneficial but not essential.
     (c)   The odorous molecules are adsorbed on appropriate sites
          on the olfactory nerve cells.
     (d)   The adsorption is accompanied by an energy change.
     (e)   An electric impulse, generated by the energy change,
          travels from the olfactory receptor to the brain.
     (f)   The brain processes the information and transmits the sen-
          sation of smell.

MECHANISMS OF PERCEPTION

A precise relationship between chemical composition and odor would
make it possible to predict accurately the odor of an unknown com-
pound or to formulate a compound with a required odor.  Recent
findings have shown that odor is closely associated with molecular
configuration, but this, according to Moncrieff  (1967), is only
half the story of odor perception.  The other half consists of the
receptor system and brain of the person doing the smelling.

An, acceptable odor theory must account for odor phenomena.  Some
of these are listed here:

     (a)   Only volatile substances are odorous.
     (b)   Air movement into the nasal cavity is necessary to feed
          the receptors.
     (c)   if air movement in the nasal cavity stops, odor sensa-
          tion vanishes.
     (d)   Water, though having the characteristics of other odor-
          ants, has no odor.
                                7

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      (e)  Gases such as oxygen and nitrogen have no odor.
      (f)  Exposure to an odor produces a high initial response and
          a declining response with continued contact. (Adaptation)
      (g)  A strong odorant completely exhausts the capacity to per-
          ceive odor in two to three minutes.  (Fatigue)
      (h)  A change in odor sometimes occurs on dilution of the
          odorant.
      (i)  Some animals have a better developed sense of smell than
          humans.
      (j)  Isomers (having the same chemical composition)  have
          widely differing smells.
      (k)  Compounds having widely differing chemical compositions
          have similar smells.
      (1)  Some odorants are perceived at a concentration of one
          millionth that of others.

The theories of odor perception differ essentially in the method
by which the "message" of the odorant is transmitted to the ol-
factory nerve.  Primary theories have proposed (a) chemical
reaction, (b) physical adsorption, and  (c) molecular vibration as
the cause of initial stimulus.

The chemical theory can be largely discounted on the basis of work
done  on a freshly severed sheep's head  (Moncrieff, 1967).  An odor-
ant was passed through the nasal cavity of the head and collected
and analyzed after passage.  The first collection of air which had
carried the odorant into the sheep's head contained none of the
odorant.  After a short time, the air passing through the head con-
tained a concentration of odorant equal to that entering.  The odor-
ant supply was cut off and the airstream continued to circulate
through the nasal cavity until no odorant was detected in the exit
air.  After a period of time, up to several hours, the air flow was
again started.  No odorant was added.  The discharged air again con-
tained the odorant in its original form.  No chemical action had
taken place.  The process of adsorption is essential in odor per-
ception.

The stereochemical theory of Amoore (1963) was first introduced
in 1952.  This theory was based upon a fit between an odor molecule
and a "socket" at the receptor site in the nose.   Seven types of
receptor sites were proposed to serve the seven primary odors—
ethereal, camphoraceous,  musky, floral, pepperminty, pungent, and
putrid.  Other odors resulted from combinations of primary odors.
Amoore has more recently altered his theory to account for a two-
dimensional rather than three-dimensional fit.  Molecular silhou-
ette of cross-sectional area is the important steric characteris-
tic of the odorant (Amoore et al.,  1967).  Odor perception is in-
itiated by an energy change brought about by adsorption of the
odorant molecule on the olfactory nerve.

Residence time of the odorant substance on the nerve is on the
order of 10"° seconds.   The time  required for the puncture to heal

                                8

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is longer—on the order of 10~4 seconds.  In the interval between
desorption and healing of the puncture, an exchange of Na  and
K+ ions occurs.  This exchange, brought about by an excess of Na+
on the exterior and K+ on the interior of the membrane, is the
stimulus for the olfactory perception process.

These theories are the basis for most of the research related to
olfactory perception in recent years.  Two points of agreement
exist in these theories:  (a) the process is initiated with the ad-
sorption of the odorant molecule on the olfactory nerve, and (b)
the cross-sectional properties of the molecule are a controlling
factor.  Again, the primary difference  is the manner by which the
characteristic odor of the molecule  is  translated to the olfactory
nerve.  It is  feasible that all these theories might be involved
in the actual odor perception  reaction.

Finally, the odorant  substance must  possess certain characteristics
if it is to be subject to the  theories  presented:  (a)  the  substance
must be sufficiently  volatile  that molecules  can be transported
to the nasal orifices,  (b)  solubility in the  lipoid material of
the mucous membrane is essential  (solubility  in water  is helpful),
and  (c) the odorous substance  must be able to be adsorbed  onto
the sensory nerve.

ODOR STRENGTH

Accurate characterization of an odor includes reference to its
strength, or  intensity,  and its quality.  ASTM  Special Technical
Publication No.  434,  "Manual on Sensory Testing Methods"  (ASTM,
1968)  effectively describes the  ground rules for  conducting odor
strength and  quality  tests.  The  manual states  requirements for
physical facilities,  test subjects,  and samples to be  tested.
Kinds of tests that may  be  applied are  discussed along with pro-
cedures for analysis  of  the data.  The  manual does not,  however,
describe the  detailed procedure by which individual  tests  must
be conducted.  Such details are dictated by the kind of material
and characteristic of odorant  being  judged.

The most common method of measuring  odor intensity is  by dilution
to extinction.  An odorant  is  diluted with an odor-free medium
until its odor can no longer be detected.  The  greatest dilution
at which the  odorant  is  just barely  detectable  is  termed its thresh-
old value.  Baker (1964)  compared four  common procedures for deter-
mining threshold  value of an odorant in odor-free  water dilution:

      (a)  Standard Method,  STD: five flasks containing serial dilu-
          tions plus  one "catch trial"  blank;
      (b)  Consistent  Series, CS:  five  flasks  containing serial  dilu-
          tions plus  two blanks;
      (c)  Triangle Test,  TT: three flasks at  each  dilution level,
          two  which are  blank; and

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      (d)  Short Parallel, SP: two flasks at each dilution level,
          one of which is blank.

Tests were run using two odorants, n-butanol and m-cresol.  Re-
sults showed that the tests, in order of decreasing sensitivity,
were TT, CS, SP and STD.  However, Baker points out that no test
is obviously superior when performed under controlled conditions
with trained personnel.

Odor intensities are stated in terms of odor intensity index,
Oil, or threshold odor number, TON.  The two values are related,
according to the equation

                           2OII = TON

Oil is defined as the number of times an odorant must be diluted
by half with odor-free medium until the threshold is reached.
TON is defined as the greatest dilution of the odorant with odor-
free medium until the threshold is reached.  Most writers seem to
prefer Oil to TON.  Certainly an Oil value of 15 is less cumber-
some and easier to grasp than the equivalent TON value of 32,768.

Odor intensity testing can be objective in nature if a sufficient
number of qualified, properly prepared observers are used and pro-
cedures and conditions of the test are standardized.  ASTM (1968)
states that the minimum number of observers for any test is five,
since any fewer places too much dependence on the response of any
one individual.  Subjects must pass a preliminary screening to
assure that they are capable of making a normal response to the
stimuli to be presented.

ODOR QUALITY

Odor quality references are often made by comparing the odor with
an odor that is familiar.  The odor is "like coffee," "like new-mown
hay," or "like a characteristic poultry odor."  The judgment of
"characteristic poultry" would depend on past experiences.  This
recollection could result from a light, well-ventilated house with
little more than the smell of must or feed to a highly populated
house with poor ventilation, high humidity, and concentrated
ammonia.  One's interpretation might include a wide range of
quality values.

Many attempts have been made to produce a list of basic odor classes
that would describe the qualities of all other odors.  Five of these
lists are presented in Table 2.  Classes of similar qualities are
placed on the same line.

Qualitative odor testing is widely used in the food and perfume
industries; however, use of qualitative odor testing in waste
treatment research is limited.  The test is often made by com-
paring an unknown with a known odorant of similar or dissimilar

                                10

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          TABLE 2.  BASIC ODOR QUALITY CLASSIFICATIONS
         AS DESCRIBED BY FIVE AUTHORS (MONCRIEFF, 1966)
Zwaardemaker
    1895
Henning    Crocker and      Amoore
  191.6   Henderson, 1927     1952
Davies
 1965
 Ambrosial                                 Musky         Musky

Balsamic or    Flowery,    Fragrant        Floral        Floral,
 fragrant      resinous                                  cedary

 Ethereal      Fruity                     Ethereal      Ethereal,
                                                         fruity
                                                        alcoholic

 Aromatic       Spicy                   Camphoraceous   Camphora-
                                                          ceous,
                                                        aromatic

                                           Minty         Pepper-
                                                         minty

Empyreumatic    Burnt        Burnt                       Almond

 Alliaceous     Foul

  Caprylic                 Caprylic

 Repulsive

Nauseating,                                Putrid
  foetid

                             Acid          Pungent
                               11

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quality in paired comparisons.  The observer then judges the
degree of similarity.

Odor quality testing lacks the desired objectivity of odor thresh-
old determinations.  Quality tests require observer judgments rel-
ative to a known odorant and subjectivity is unavoidable.

LIMITATIONS OF ODOR TESTING

The limitations of odor testing arise from the existence of odor
phenomena and the preferences (or subjectivity) of observers.
The sources of these limitations will be discussed individually.
It should be apparent how each limitation can enter into the inter-
pretation of odor test results.

Adaptation

Adaptation is the adjustment of the observer to an odor stimulus.
The level of sensation diminishes with time even though the stimulus
is applied at a steady rate.  Observers who enter the vicinity of
lagoons soon lose the ability to make unbiased odor judgments about
odorants similar to those of the immediate environment.  Rate
of adaptation varies with strength of the stimulus.  Moncrieff
(1966) demonstrated adaptation as follows: a subject who first
took a sniff of pure acetone could recognize nothing less than
5.0 percent acetone with a second sniff.  This is 170 times thresh-
old concentration.  The effect of a dissimilar odor was not so
great—after smelling pure acetone, n-butanol could be recognized
at 0.06 percent, or 12 times threshold concentration.

Fatigue

Fatigue is the result of adaptation.  Exposure to a strong odorant
may completely exhaust the capability to sense odor.  Fatigue de-
velops gradually, with an exposure of two to three minutes required
for total exhaustion.  Recovery after removal of the odorant re-
quires about the same length of time.  Fatigue is selective—that
is, fatigue to one odor will reduce sensitivity to similar odors,
but does not produce fatigue for all odors.

Odorant Concentration

Changes in odor quality sometimes occur due to dilution.  For ex-
ample, concentrated furfuryl mercaptan has a nauseating odor but
is reminiscent of the aroma of coffee when greatly diluted.

Moncrieff (1967) reported the concept of limiting intensity, which
states that the human nose cannot distinguish between odorant con-
centrations greater than saturation level.  The reasoning behind
this concept (which heavily supports the adsorption theory of od-
or) is that receptor sites become filled with odorous molecules
and an increase in the number of molecules inspired causes no in-
crease in sensation.
                               12

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Mixtures

Rosen et al. (1962)  listed four possible reactions for mixtures
of two individually odorous components.  In these equations, R,
is the odor stimulus of component A, RB is the odor stimulus or
component B, and RA+B is the odor stimulus from the combination of
components A and B.

     (a)  Independence      RA+B = RA or RR


     (b)  Antagonism or     RA+R < R  or R
          counteraction        B    A     B

     (c)  Additions         R, ._ = R, + R
     (d)  Synergism         Ra   > R  + R
                                    A
Moncrieff (1967) described counteraction (the mutual discrimina-
tion) of two odors to the extent that they are odorless in combin-
ation.  Using low concentrations of the odorants they tended to be
additive, but with certain combinations of high concentration,
the effect was no odor.  Guadnagni et al. (1963) found that com-
bination of sub-threshold concentrations of odorous components
produced suprathreshold mixtures .

Without prior knowledge, the quantitative and qualitative outcome
of the reaction of the combination of two components is unpredict-
able.  In addition, the type of reaction can vary according to the
concentration of the components.

Anosmia and Parosmia

Anosmia  (odor-blindness) is a condition which affects about 10 per-
cent of the population.  Partial anosmia — anosmia for a group of
similar odors — is more common than complete anosmia.  Partial anos-
mia is much more likely to exist without the knowledge of the af-
flicted person.

Parosmia  (perversion of odor) is a second type of olfactory di-
sease: the parosmatic senses a different odor than the one put
before him.  The perverted odor is often an unpleasant one.  But
the condition is likely to be temporary.

Pungence

"Pungent" has been included as a basic odor type by some authors.
This classification is associated with strong acidic and basic
smells.  Pungence may not be a true olfactory nerve response, but
a sensation of pain caused by irritation of the trigeminal nerves
in the nasal* cavity.
                                13

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SUPPLEMENTARY INSTRUMENTS

The gas-liquid chromatograph, GLC, has been the most important in-
strument in supplementing the capabilities of the human nose in
odor research.  While the nose can best determine quality and
intensity of simple and complex odor combinations, the GLC can
best fractionate and quantify the odorant components involved.
The capabilities of one do not replace the capabilities of the
other.  Advancing technology has made possible increased sensi-
tivity of the GLC and, therefore, greater capacity to identify
the trace quantities of odorants in complex materials.

Odormeters of various forms have been developed to assist in test
work.  The meters are of the general form that can make the neces-
sary dilutions of odorant with odor-free air prior to inspiration.
The meters have been given such names as "osmoscope," "odormeter"
and "osmometer."

Development of a "mechanical nose" which would eliminate errors
of natural variation and subjectivity in the human nose would
greatly advance odor research.  The most important obstacle in such
a development continues to be the lack of understanding of the
odor perception process of the human nose.  Even so, several re-
searchers have made efforts to duplicate the human nose.
                               14

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                            SECTION 4

                QUANTITATIVE MEASUREMENT OF ODORS


The technological difficulties of quantitative odor measurement
are formidable.  Odor is essentially a subjective response to the
mixture of chemical compounds present in air.  This response is
a function not only of the chemical make-up of the air but also
of the psychological disposition of the observer as well.  Eval-
uation of an odor is thus a complex physiological and psychological
process and it is little wonder that techniques for quantitative
measurement of odors have been fraught with difficulty.  Two sep-
arate aspects of odor can be identified: strength or intensity,
and quality.

ODOR STRENGTH

Odor strength or intensity is the more direct and easily measured
of the two aspects.  The current concept is that each odor source
may be diluted sufficiently with odor-free air to be indistinguish-
able from odor-free air by the human nose.  That concentration
barely distinguishable from odor-free air is termed the thresh-
old odor.  The strength of an actual odor can be defined in terms
of the number of dilutions with odor-free air required to reduce
the odor to threshold concentration.

Similar to the air dilution technique is a liquid dilution method.
In this technique, a sample of odorous material is mixed with odor-
free water.  Generally, the diluted sample along with some odor-
free samples  is offered to a panel of observers.  The general
technique is to make up a series of five bottles for the panel,
two containing a dilution of odorous material, the other three
containing odor-free water.  Panel members are asked to mark on
their score sheets those bottles which contain the odorous mate-
rial.  By making a series of dilutions and offering them to the
odor panel for evaluation, minimum detectable concentrations of
the material can be determined.  By making liquid dilutions suc-
cessively greater by a factor of two, resulting data can be used
to determine an odor intensity index (Oil).  In other words, if
threshold odor were determined to be diluted 15 parts odor-free
water to one part odorous solution, the odor intensity index would
be 4.  A second means of expressing this information would be a
threshold odor number (TON), which is equal to two raised to the
odor intensity power.


                               15

-------
 SCENTOMBTER

 Field measurements of odor intensity are difficult because of the
 lack of an overall acceptable measuring device and the inability
 of people to accurately describe odors.  One device on the market
 for the estimation of odor intensity is the scentometer.  The
 scentometer is essentially a rectangular, plastic box containing
 two air inlets  (one for each activated charcoal bed) and four
 odorous air inlets (1/16-, 1/8-, 1/4- and 1/2-inch in diameter).
 The odorous inlets are directly connected to a mixing chamber and
 the nasal outlets  (see Figure 1).

 In field operations, the observer takes the scentometer where
 odor intensity is to be measured.  He places the device to his
 nostrils, covering the odorous air inlet ports with his fingertips
 and breathes through the instrument to adjust his sense of smell
 to odor-free air.  The concept is that any air entering his nostrils
 under these conditions will have passed through the activated char-
 coal bed and be odor-free.  Once the observer's sense of smell
 has become acclimated to odor-free air and his sense of smell rested
 to the point of maximum sensitivity, the ports are opened in suc-
 cessively larger diameters beginning with the 1/16-inch port.  He
 continues in this manner until an odor is first detected coming
 through the device.  The design is such that odorous air is mixed
 with filtered air in definite proportions so that recording the
 port or ports opened at the time an odor was first detected pro-
 vides a measure of the dilutions required to reach the odor thresh-
 old.  Table 3 provides a correlation between the ports which are
 open and the calculated dilution entering the nostrils of the
 observer.  With a combination of ports open, it is possible to
 estimate odor dilutions through thresholds ranging from 1.47 to
 170.  Measurements made with more than one port open, however,
 are subject to  question because of the frequent inability of the
 observer to detect small differences in odor intensity.

 In discussing the scentometer, Huey et al. (1960)  stated that their
 experience had shown that odors about seven dilutions to threshold
 would probably cause complaints while those measuring 31 dilutions
 to threshold could be described as a serious nuisance if they per-
 sisted for a considerable length of time.  The scentometer was
 described in a paper by Rowe (1963), in which he indicated that
 it required about ten times the perceptible odor threshold to give
 a definite sensation of odor and another tenfold increase to be
 considered a strong odor.

 The scentometer has received rather widespread application in ani-
mal waste odor evaluation.  In spite of its application, however,
 there are several basic limitations to this approach.  The sensi-
 tivity of the observer is highly Important in determining the
 values achieved with the instrument.  Although sniffing through
 the scentometer with odorous air ports closed is designed to re-
 store normal sensitivity, complete restoration of sense of smell

                               16

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ODOROUS AIR
INLET PORTS
J_" J_"_L'_LM
2  4  8 16
                                                                   SNIFFING
                                                                    PORTS
SCREEN  ON BOTH
SIDE  OF CHARCOAL
FILTER
                        Figure 1.  Scentometer

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             TABLE 3.  DILUTION  TO  THRESHOLD  VALUES
            WITH VARIOUS PORTS OPEN ON A  SCENTOMETER
               WHEN AN ODOR  IS BARELY DETECTABLE
Dilutions
to threshold
1.47
1.49
1.55
1.88
2.0
5.55
5.75
6.75
7.0
27.0
31.0
170.0

1/2 in.
0
0
0
X
0
X
X
0
X
X
X
X
Odorous
1/4 in.
0
0
0
0
X
0
0
X
0
X
X
X
air inlets1
1/8 in.
0
0
X
X
X
0
0
0
X
0
0
X

1/16 in.
0
X
X
0
X
0
X
X
X
0
X
0
Definition of symbols:  x indicates port is covered
                         0 indicates port is open
                               18

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may not occur rapidly enough to obtain meaningful results.   The
charcoal bed can become saturated and not give complete odor removal
from air breathed when all ports are closed.  Since there is no
indicator to show if the carbon is saturated, misleading results
may be obtained under these conditions.  Intermittent odors common
in animal waste, particularly when observations are made some dis-
tance from the odorous source, present additional difficulties and
require use of the scentometer over a considerable period to obtain
representative data.  In spite of these limitations, however, the
scentometer is a useful device, and is being used by some regulatory
agencies.

ODOR QUALITY

In contrast to odor strength, which can be quantitatively evalu-
ated, there is no straightforward technique to quantify odor qual-
ity.  Most frequently, odor quality is described by comparison to
a common odorant or sensation with which the reader or listener
is familiar.  For example, White et al. (1971) used the following
terms to describe the odorous components of dairy waste: foul,
sweetish, acetate, nutlike, pungent, and musty.  In describing the
odor of various fractions of poultry manure odor, Burnett  (1969)
used the following words: rotten egg, rotten cabbage, onion-like,
putrid, butter-like, and garlic.

An alternative method to evaluate odor quality was used by Sobel
(1971). He asked a panel of odor evaluators to select a number from
one to ten, indicating the degree of offensiveness of the  samples.
A nonoffensive odor was marked as 0, a very strong offensive odor
was ranked as 10, a definite offensive odor was 6 and a faint of-
fensive odor was 4.  He also asked panel members to select suitable
descriptors from the following list to describe the odor of the
sample: mold, musty, fish, stagnant water,  sulfide, rotten egg,
petroleum, earth, yeast, ammonia, grain, feed, sour, fermented,
and cabbage.  Using this approach, he was successful in differ-
entiating the offensiveness of an odor and  the odor strength.
                                19

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                            SECTION 5

             RELEASE OF AMMONIA AND HYDROGEN SULFIDE
                      FROM LIQUID SURFACES


Wastewaters of both human and industrial origin contain sulfurous
and nitrogenous compounds which have been implicated as contribut-
ing to odor production.  Under anaerobic conditions/ these com-
pounds are typically converted to their most reduced states, hy-
drogen sulfide and ammonia.  The evolution of these compounds has
been investigated from a physical chemistry perspective in work
summarized in a paper prepared by C. N. Click and J. C. Reed,
entitled "Atmospheric Release of Hydrogen Sulfide and Ammonia from
Wet Sludges and Wastewaters."  Their material has been utilized in
this discussion where it is pertinent to the release of odorous
materials from anaerobic meat packing plant lagoons.

HYDROGEN SULFIDE IN WASTEWATER

The evolution of hydrogen sulfide (H~S) from sewage has been docu-
mented frequently for sewers and sewage works.  Gravity sewers and
force mains may produce soluble sulfide buildup to the point of odor
nuisance, sewer corrosion and toxicity.  Gravity sewers are less
subject to sulfide production than force mains since more oxygen
is available in gravity sewers and oxygen must be virtually absent
from sulfide accumulation.  Oxygen will eventually cause previously
formed sulfides to be converted to soluble non-volatile sulfates.
Iron and other heavy metals form insoluble suspensions of sulfides
which are not normally available for reaction of H-S.

In most wastewaters, sulfides are usually present in both organic
and inorganic forms.  The organic "sulfides" are not measured by
the usual wastewater tests, but they may contribute to nuisance
conditions either by being odorants (such as mercaptans, thio-
ethers, and disulfides), or by contributing soluble "inorganic"
sulfides via biological degradation.  Inorganic sulfides may
be measured by the standard wastewater tests but not all of the in-
organic sulfides may be available for H_S formation because as noted
above, some heavy metals form insoluble sulfides.

The threshold of taste/odor detection of H~S is reported to be
between 1.0 x 10~  and 1.0 x 10T  mg/1 in water (Pomeroy and Cruse,
1969)  and to be about 4.7 x 10~  ppm in air (Leonardos et al., 1969)
where ppm is by volume.  The threshold limiting value  (TLV)  for
H_S in air is 10 ppm, but various studies (WPCF, 1969)  have reported
                               20

-------
that 100 to 300 for one hour is the maximum tolerable short-time
exposure or even that 300 ppm can cause death in a short time.  The
low concentrations of taste/odor detection practically guarantee
that odor nuisances exist before toxicity problems can occur and
sometimes before noticeable corrosion results.  Unfortunately/ the
higher  (greater than 10 ppm) concentrations render the olfactory
sense ineffective very quickly, so workers are not continuously re-
minded of the danger.

AMMONIA IN WASTEWATER

The evolution of ammonia  (NH,.) from wastes is possible.  Meat pack-
ing wastes contain nitrogen in several forms.  Most is derived
from the breakdown of the organic materials, urea and protein.
The thresholds of odor detection and recognition of ammonia
have been reported  (Stahl, 1973) to be between about 0.04 and 47
ppm.  It is interesting that although the 1972 TLV for ammonia was
given as 50 ppm, proposed changes would reduce the TLV to 25 ppm.

HYDROGEN SULFIDE EVOLUTION AND pH

Substances that react in  aqueous solution to accept or donate
electrons  (and consequently donate or accept protons) dissolve to
a degree dependent upon solution pH and their dissociation con-
stant  (K).  For an acid the equilibrium dissociation constant,
K, is defined by the dissociation equation, which  for H2S is:

                    H2S JHS~ + H+                         (1)

The subsequent dissociation of the bisulfide ion  (HS ) into  a
second proton and sulfide ion with a second constant need not con-
cern us because neither the bisulfide nor sulfide  ion  (S~) has
an odor or vapor pressure itself.  lonization as in equation (1)
removes the reactive gas  from gas-solubility considerations.
Equation  (1) gives K, by  definition:

                                                          (2)
where    symbolizes concentration  in  mol/1.

By rearranging equation  (2)  and  substituting the  definition for
pH and pK  (analogous  to  pH), we  obtain:

                   pH =  pK1  +  log  f HS~l/["H2s"j             (3)

Equation  (3)  is  the relationship between the pH of a solution
of hydrogen  sulfide and  the  concentrations of the dissolved gas
 (H-S) and  the bisulfide  ion  (HS  )  in  solution.

Defining the total soluble  sulfide concentration  (before reacting)
as C, and  f,  as  the fraction dissociating at equilibrium, the con-

                               21

-------
centiraticms become H2S =  (1  -  f,)  C,;  HS~  =  H+ = f-,C,.   Substitut-
ing these values  in equation (37  ana  taking  antilogs:


           (f) =  10 exp  (pH  -  pK-/fl  +  10 exp (pH  - pKl   (4)
                                and
              (1 - f^ = l/[l +  10 exp  (pH  - pK^J            (5)
Equation  (4) gives the bisulfide ion and  (5)  the  gaseous  hydrogen
sulfide fraction for a solution with any  total  soluble  sulfide  con-
centration as a function of solution pH.   Equations  (4) and  (5)
thus give the fraction of dissociated  and undissociated hydrogen
sulfide,  respectively.

Equations (4) and  (5) show that at a given temperature, the  per-
cent dissociation of a reactive gas is controlled entirely by the
system pH (providing there are no competing reactions)  and can  be
calculated for chosen values of pH from the pK  alone.   For hydrogen
sulfide, pK., = 7.02 at 25 °C and thus  at  a solution pH  of 7.02.


             (1 - f1) = 1/(1 + 10°'0)  = h = 0.5             (6)


When solution pH = pK the solute is 50 percent  undissociated
(gas) and 50 percent dissociated  (ions) .   Increasing acidity (low
pH) will result in higher values of  (1 -  f,)  or undissociated H?S
over wastewater solutions.

AMMONIA EVOLUTION AND pH

The relationship between the evolution of ammonia and pH  is  analo-
gous to that for hydrogen sulfide.  Consider  the  equilibrium reac-
tion between ammonium ion and gaseous  ammonia:


                         NH4 + J NH3 -I- H+                      (7)


If the original concentration of ammonia  is C~, and f-  is the frac-
tion reacting,  at equilibrium we have:


                     K2 = [NH3J[H+] f [NH4+]                 (8)

                   pH = PK2 + log [NH3J/ [NH4+]              (9)


        f2 = 10 exp (pH - pK2)/j"l + 10 exp (pH  -  pK2)l        (10)

                               22

-------
where K,, = the equilibrium dissociation constant for ammonium ion.

Equation (10) is analogous to  (4) but equation  (10) gives the frac-
tion  (f~) of undissociated ammonia  (gas) present whereas equation
(4) gives the fraction  (f,) of dissociated sulfide  (ions) present.

SOLUBILITIES OF UNDISSOCIATED GASES

Henry's Law states that the solubility of a gas in  a liquid is
directly proportional to the partial pressure of the gas over the
liquid for ideal  solutions.  Providing there is no reaction be-
tween solute  (gas) and solvent  (water), Henry's Law should be fol-
lowed reasonably well for the dilute solutions encountered in waste
treatment.  Henry's Law may be written:

                             P = Hx                      (ID

where P = vapor pressure of gas in equilibrium with solution
      H = Henry's Law "constant," pressure per mol  fraction,
          usually atmospheres/mol fraction
      x = mol fraction of undissociated solute in  the  liquid phase.

Equation (5) provides the fraction of total soluble sulfide present
in solution as undissociated  (gaseous) H S as a function of pH  and,
coupled with a knowledge of the total soluble sulfide  concentration
(C,), can be used to calculate the mol fraction of  gaseous hydro-
gen sulfide in solution:

                               (l-f,)C,/32.06
                                  1  X            	     (12)
                 1    (l-f1)C1/32.06  +  (s.g.)106/18.02


where       x  = mol  fraction hydrogen  sulfide  gas  in  solution
         (l-f,j = fraction of total sulfide present  in  solution as
                 gas
            C, = total soluble  sulfide  concentration in solution,
                 mg/1
          s.g. = specific gravity of water at temperature,  t.

Substituting  (5) into  (12)  and  the result into  (10), we have:

                                  exp(pH-pK, )] ]  C,/32.06
                                  	£J-J	±—	,  atm
                   exp(pH-pK, )1? C,/32.06 +  (s.g.)10 /18.02
                             1J3  1                           (13)

From  (13) one  can estimate  the  concentration of gaseous hydrogen
sulfide  (C  )  in the  air over the wastewater at constant tempera-
ture, sincef at one atmosphere:


                    C  , = 10  (P.. ,,/l.Q),  vol
                      gl         H2S

                               23

-------
           *Cgl = lo(32-06/29-°)pH s/1-0/  
-------
                pK2 = 0.2401 + 4756/T + 41097/T'
                                                  [20)
The pK's of ammonium ion and hydrogen sulfide decrease with in-
creasing temperature.  Thus the K's of NH.  and H2S increase with
increasing temperature and equations  (1) and  (7) are forced to the
right.
                  )  and (7) ,  one can see that H2S tends to
                  whereas NH.. tends to be formea from the
Referring to equations
dissociate into HS  + H
dissociation of NH.  into NH., + H  as the temperature increases
(that is, as the respective K's increase).  Thus these effects
are opposite, and increasing temperature at constant pH increases
the proportion of NH  gas in solution but decreases the proportion
of H2S gas.  Figure Z, a plot of the fraction undissociated gas
(for both H_S and NH..) versus pH for several temperatures, shows
this effect graphically.  Referring to Figure 2 at pH 7, H2S is
about 75 and 50 percent undissociated at 40 and 80  F, respectively
whereas at pH 10, NH  is about 55 and 85 percent undissociated at
40 and 80 °F, respectively.

LABORATORY RESULTS

A series of laboratory studies concerning hydrogen sulfide pro-
duction in anaerobic lagoons was conducted by Gloyna and Espino
(1969) .  Their model was based on units 58 cm in diameter and 175
cm deep.  Illumination was provided 12 hours daily.  Four indepen-
dent variables were investigated: organic surface loading rate,
detention time, surface loading rate of sulfate, and influent
sulfate concentration.  Results are shown in Table 4.  Based
upon these data, an empirical relationship of the following
form was devised:
               S  = a
                BOD surface
                loading rate,
                Ibs/acre-day
SO. influent
concentration,
mg/1
where
           _   /detention time,! +
               {days           j

S= = sulfide concentration, mg/1
 a = -0.011
 b = 0.025
 c - -0.08
 d = 3.3
                                                          (21;
These coefficients were based on the model they used and a tem-
perature of 25 °C.  This equation was further refined to correlate
the data in the form:
     S  = I 0.0001185  (BOD surface loading rate)

           - 0.001655 (detention time) + 0.0553
                                                        SO
                                                           =  (22;
                               25

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                                                         1.0
                         SOLUTION pH
Figure 2.  Effect of Solution pH  on  Fraction Dissolved Reactant
           Dissociated (Ionized).
                               26

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    TABLE 4.   RESULTS OF LABORATORY ANAEROBIC LAGOON STUDIES
                TO PREDICT SULFIDE CONCENTRATIONS
                    (GLOYNA AND ESPINO,  1969)

Test
#
1
2
3
4
6
7
BOD
load1
136
68
136
136
68
136
SO.,
cone. ,
mg/1
23
23
23
206
200
400
SO,,
load1
11
11
23
94
188
188
Detention
time, days
30
30
15
30
15
30
Sulfide
concentrations ,
mg/1
0
0
0
4
6
8
.432
.500
.12
.29
.36
.76

founds per acre-day
Furthermore, Gloyna and Espino  (1969) defined the rate of hydrogen
sulfide transfer from the liquid to the atmosphere using the Fickian
relationship:

                         q = k(c - ceq)                   (23)

where     q - mass rate of exchange
          k = diffusion constant
          c = hydrogen sulfide  concentration
        c   = hydrogen sulfide  concentration at equilibrium.
A series of tests was conducted in model lagoons to measure the
hydrogen sulfide escape rate.  Experiments were conducted in
basins 175 cm deep, having a surface area of 188 sq cm.  So long
as_pH of the basins was less than 4.5, there was essentially no
HS  nor S= to confound the issue.  Under these conditions, the
data could be analyzed as follows:
                                27

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                                 -
                            at ~   v

                            dc
                                                          .„ _ .
                                   ^                      (25)


                          In c = In CQ - k|t              (26)

where    c = hydrogen sulfide concentration, mg/1
        c  = initial hydrogen sulfide concentration, mg/1
         t = time, hr
         A = surface area, m
         V = liquid volume, m
         k = hydrogen sulfide escape rate, m/hr.

The value of the calculated rate was approximately 0.006 m/hr for
tests with low pH, low wind movement, and no waves.  Wind effects,
waves or other agitation would be expected to increase k values.

Based upon these experiments and the equations developed, Gloyna
and Espino established design levels of sulfide in the lagoon as
4 mg/1.  Detention time or loading rates may be adjusted to meet
this criterion.
                               28

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                            SECTION 6

              ANAEROBIC LAGOON DESIGN AND OPERATION
The anaerobic lagoon is the treatment process most widely used at
meat and poultry plants.  The anaerobic process is especially
suited to the concentrated hot wastewaters from these plants.
The process utilizes anaerobic bacteria, which function in the
absence of free oxygen to break down organic wastes.  The lagoon
is usually deep and covered with a blanket of floating sludge.

The anaerobic lagoon has obtained greater than 90 percent BOD
reduction, with highest removals during warm weather.  The process
has minimum capital and operating costs, is simple to operate,
and shows visible treatment results; mechanical equipment is not
necessary and treatment processes can withstand the shock load-
ings common to the industry.  The anaerobic lagoon has given high
removal efficiency with and without sludge recirculation.

There are two potential problems in the selection of anaerobic la-
goons for meat packing wastes: odor emission, and high ammonia con-
centrations in the effluent.  Ammonia is toxic to fish, and  fish
kills due to ammonia in meat plant discharges have been documented.
However, reduction of protein to ammonia  is a reaction that can-
not be avoided in biological systems.  The problem is that the
concentration of ammonia in anaerobic lagoon effluents requires
selection of additional treatment processes to remove the ammonia.

Anaerobic lagoons are designed with a low surface area to volume
ratio to conserve heat and minimize surface re-aeration.  Depths of
10 feet or more are desirable.  Economic considerations and main-
taining several feet above groundwater usually limit depths  to
less than 18 feet.  The volume is based on organic loading and de-
signs range from 12 to 25 pounds of BOD- per 1,000 cubic feet with
15 pounds 600^/1,000 cubic feet being common.  The anaerobic la-
goon at the Reeves plant in Ada, Oklahoma, has a water depth of
Io feet and an organic loading of 12 pounds BOD^/1,000 cubic feet
(Witherow, 1976).  Average removal efficiencies for BOD5, TSS, and
FOG were 92, 84 and 95 percent, respectively.

The parameters used in determining municipal sewer rates are gen-
erally BOD, TSS, and oil and grease.  The BOD and TSS concentrations
at the Reeves plant were below 200 mg/1 except on rare occasions
(Witherow, 1976).  Oil and grease analyses made on  the effluent

                                29

-------
 showed  concentrations were well below the  100 mg/1  limit  commonly
 used by municipalities.  The high percent  removal and consistency
 of effluent concentration from the anaerobic lagoon resulted  in an
 effluent which would meet common limitations for discharge to a
 municipal treatment plant.

 Discharge of an effluent with high hydrogen sulfide concentrations
 to a municipal system will damage concrete sewers and structures
 unless precautionary devices are installed.  Hydrogen sulfide,
with its characteristic rotten egg odor, can be detected  at low
 concentrations.  At the demonstration facilities, the sulfate
 concentration in the water supply was 4 mg/1 and a hydrogen
sulfide odor could not be detected.  Other septic odors could only
be detected within 50 feet of the anaerobic pond in the downwind
direction.

Most of the effluent ammonia concentrations were between  65 and
 85 mg/1 at the Reeves plant (Witherow, 1976).  The conversion
of protein to ammonia increased the concentration of ammonia three-
fold through the anaerobic pond.

Oil and grease concentrations in the raw waste and effluent of
the Reeves plant averaged 514 mg/1 and 16 mg/1, respectively,
which shows limited loss in the packinghouse  and high reduction in
the anaerobic pond (Witherow, 1976).   A grease cover did  not form
on the anaerobic pond,  which indicates grease was being digested
in the pond.  Some consideration was given to mixing grease and
straw on the surface of the lagoon to form a cover to reduce heat
loss.  This was not done and temperature reduction through the
pond was high during part of the winter when  water temperature
dropped to around 10 °C.  Insignificant changes in removal ef-
ficiencies were noted during this period.  In a colder climate
there are greater advantages for a cover to reduce heat loss and
maintain biological activity.

The anaerobic lagoon can produce an effluent  suitable for discharge
to a municipality, but  it will not produce an effluent suitable
for discharge into a surface water without further treatment in an
aerobic process.   Aerobic processes are those that maintain dis-
solved oxygen in the water.
                              30

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                            SECTION 7

                        FIELD EXPERIENCE
In an attempt to identify specifically documented odor problems
related to anaerobic lagoons serving meat packing plants, it was
necessary to contact personnel who have had immediate experience.
No reports were found in the literature which specifically docu-
mented odor problems or their solution.

DOCUMENTATION OF ODOR PROBLEMS

Odors in the proximity of a meat packing lagoon near Sweetwater,
Texas, were made by Dr. John M. Sweeten (personal communication,
Texas A & M University, 1976).  In connection with that lagoon,
odor measurements were made utilizing the scentometer.  Downwind
of the anaerobic lagoon three readings were made indicating an
odor intensity of 31 dilutions to threshold.  The odor was
described as a mixture of normal, musty lagoon odors sparked with
releases of putrid odors whenever large bubbles or mats of scat-
tered floating solids were surfacing.  The lagoon at the location
has a volume of 97,000 ft3 and a daily BOD5 input of 310 pounds
for a loading of 3.2 pounds BODs/day/l,000 ft3.  This is not a
high loading rate; however, in conjunction with the high sulfate
concentration in the city water  (218 mg/1), problems were created.
In this particular case, aerators have been suggested as a poten-
tial remedy.

Odor measurements were also made in the vicinity of an anaerobic
meat packing plant lagoon near Eagle Pass, Texas, (Sweeten, per-
sonal communication, Texas A & M University, 1976).   Odor inten-
sities adjacent to the lagoon were 31 dilutions to threshold.  At
a distance of 0.75 miles downwind, odors were detectable at a
concentration of two dilutions to threshold.

In discussing an anaerobic lagoon for the treatment of meat
packing plant wastes in New Zealand, Rands and Cooper (1966)
identified "one of the main criticisms of open, large scale,
anaerobic digesters as being the liability arising from the as-
sociation of hydrogen sulfide with digester gas."  Other trace
constituents such as mercaptan and amine-type volatiles joined with
hydrogen sulfide to produce a difficult-to-define "pond" odor.
"Uncontrolled meat waste digesters have attracted considerable
attention in New Zealand in the past because of blackened house
paint, sometimes at distances of several miles from the source."
(Rands and Cooper, 1966).
                               31

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 At Moerewa,  New Zealand,  odor control is dependent on as continuous
 a scum cover as possible  on the sludge pit (Rands and Cooper,
 1966).   The  scum cover effectively blankets all unpleasant odor,
 but how much of this is purely physical retention and how much
 oxidation of sulfide as gases permeates the porous scum mat is not
 yet known.   But the  latter process appears to play an important
 part in reducing gaseous  sulfide.

 The effectiveness of the  scum mat  in reducing the escape of hydro-
 gen sulfide  to  the atmosphere was  demonstrated by tests carried out
 over the  surface of  the digester using a Drager Gas Detector held
 four inches  above the  water or scum surface.   The concentration of
 hydrogen  sulfide above the scum, which was about one inch thick,  av-
 eraged  0.35  mg/l/v/v compared with concentrations of 2.0 to 15.0
 mg/1 over scum-free  areas  (Rands and Cooper,  1966).

 "Despite  many attempts, the collection of a representative sample
 of gas  reaching the  surface of an  open digester has so far pre-
 sented  insuperable difficulties.   Any negative pressure used in
 collection increases  gas evolution and even collection under a bell
 jar can encourage increased solution of carbon dioxide.   This  is
 suggested by the analysis  of a sample gas collected in this way and
 analyzed  by  the Chemical  Inspector,  Department of Health,  Auckland,
 with the  results shown in  Table  5."   (Rands and Cooper,  1966).
   TABLE 5.  ANALYSIS OF A GAS SAMPLE COLLECTED IN A BELL JAR
          OVER AN ANAEROBIC MEAT PACKING WASTE LAGOON
                         IN NEW ZEALAND
                     (RANDS AND COOPER, 1966)
      Gas                              Concentration,
                                     percent by volume

Hydrogen sulfide                            0.4

Carbon dioxide                              7.0

Methane                                    85.0

Oxygen                                      0.6

Nitrogen                                    7,0
  and undetermined
                               32

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 A continuous  record  of  the  concentration  of  hydrogen  sulfide  in
 the  air^was kept  at  the  treatment  plant close  to  the  digesters
 and  sedimentation tanks  from March to  November, 1965  by  a paper
 tape sampler  using lead  acetate-impregnated  paper.  Another sampler
 was  placed at the outlet of the  oxidation pond to the river and  a
 continuous record was kept  there from  March  to June,  1965.

 The  highest concentration was  an isolated peak of 0.11 mg/1 but  of
 the  total number  of  readings  at  intervals of two  hours,  1,897 were
 nil  and  389 positive with an  average of 0.2  mg/1.   Similar results
 were obtained from the other  sampler,  although an occasional high
 of 0.20  mg/1  at night indicated  a  local anaerobic "boil" from the
 digester under still air conditions.

 The  experience gained from  these records  has shown  that maintenance
 of gas lines,  a continuous  scum  layer  on  digesters, as little dis-
 turbance of the digester surface as possible,  and sufficient wind
 to disperse odors  without disturbing surface scum are important
 details  of odor-free operation  (Rands  and Cooper,  1966).

 A  municipally owned anaerobic  lagoon (Monmouth, Illinois) receives
 waste from a  Swift and Company packing house killing  an average
 of 8,000 head per  day at maximum capacity.   This  plant discharges
 between  1 and  1.5 million gallons  of liquid  waste per day to a
 treatment system  with a  BOD5 ranging from between 600 to 700
 pounds per day.   The anaeroBic lagoon  has a  surface dimension of
 400  by 260, which  is covered by  a  hypalon cover.  At  this plant,
 the  gas  is not utilized;  it is burned  off as produced.

 Mr.  Robert Merwin, Plant  Operator, indicated they have found it  a
 successful operation.  They do not attempt to  store gas beneath  the
 cover but keep it burned  off as  produced.  Their  cover has neither
 ropes nor cables but is  a free-floating device; the goal is to
 have the cover rest directly upon  the  water.   The major problem,
 according to Mr. Merwin,  is that excess grease  and  fat tend to form
 a  scum and stick  to the  cover.

 The  cover for the Monmouth, Illinois,  anaerobic meat  packing waste
 lagoon was manufactured  and installed  by  Globe Liner  of Long Beach,
 California.  Mr. Bill Kays of that company reported that Globe
 Liner has manufactured and installed over 90 covers and that they
 are working satisfactorily.  It  is his opinion that the success
 enjoyed by Globe Liners  has been largely due to his company's
 special  skills both in design and  fabrication  of  covers.

 This particular cover is  fabricated from  a hypalon resin produced
 by Dupont and Company of  Louisville, Kentucky.   Although they
 do not serve meat packing lagoons other than the Monmouth, Il-
 linois, currently in operation,  they have several meat packing la-
 goon covers under design.  In discussing  the cost of  hypalon covers
which might be installed, Mr. Kays pointed out that it is diffi-
 cult to determine a representative cost; however,  he  was able

                               33

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 to  indicate that the typical cost per square  foot for a moder-
 ately large lagoon is in the neighborhood of  $1.75 to $2.50 per
 square foot.  This price does not include the gas collection
 system nor unusual installation or production difficulties.
 According to his recollection, the Monmouth cover was installed
 for a cost of approximately $1.90 per square  foot three years
 ago.

 Mr. James Chittenden, Vice-President of Research and Development,
 Texas Amarillo Systems Company, was contacted relative to his ex-
 perience with respect to meat packing anaerobic lagoon covers.
 Mr. Chittenden has been involved with meat packing lagoons for
 some time and has been watching the Monmouth, Illinois, operation
with considerable interest.  He reports that his firm is currently
 designing an anaerobic lagoon to be located in Arizona which will
 be provided with a cover for a plant killing 1,400 animals per day.
 This plant will utilize water with a sulfate content of 390 mg/1.
 It is his design that the lagoon will produce an average of 100
 cubic feet per minute of gas having a heating value of 650 Btu/lb.
He anticipates the system will, therefore, be sufficiently large to
 justify the purchase of a boiler to utilize the gas for heat
production.  Anticipated cost of the cover is to be in the range of
 $250,000.

The University of Illinois has used a "Controliner," Model 90,
Environetics Company, as an anaerobic swine manure lagoon liner
 and cover for two years (Waldo, 1976).   This unit provides total
containment of both liquids and gases.   Prior to installation of
 the unit, both odor and mosquito problems existed.  Periodic
analyses of gases within the Controliner were performed by gas
chromatography and are summarized in Table 6.  As the table shows,
methane content of the gas is a sensitive function of temperature.
                               34

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TABLE 6.  COMPOSITION OF GASES  INSIDE  THE  CONTROLINER
        AT UNIVERSITY OF ILLINOIS SWINE FARM
                    (WALDO, 1976)

Constituent, percent
Date
September 1, 1976
September 1 5
October 4
October 17
October 24
December 5
March 19, 1977
April 1
April 23
July 29
co2
34.8
45.0
42.0
18.0
25
5.6
11.7
6.8
50
20
°2
2.5
4.5
2.0
12
4
19
17.2
19.0
9.7
10
N2
20.7
10.5
10.5
45
46
72
71
73
39
49
CH<
42
42
48
25
25
2
4
1
1
21
1





.9
.8
.2
.2

                          35

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                            SECTION 8

                        PROBLEM ANALYSIS
Odors are a complex subjective phenomenon.  Odor problems arise
when neighbors or other people who live, work or recreate in
the vicinity of a source of air emissions register complaints or
otherwise bring pressure against an  operation.  Legal recourse is
available for property owners whose use of their property has been
unreasonably restricted due to odors.  Where large groups of people
have been offended, public nuisance suits have been filed seeking
operational restrictions or actual plant closure.

Anaerobic lagoons, which have proven to be low cost effective waste
treatment devices, are especially prone to emit odorous gaseous com-
pounds.  By its very nature, the anaerobic process degrades complex
organic compounds in the absence of oxygen to less complex com-
pounds of increasing volatility.  Both the end products and inter-
mediate breakdown products contribute to odor production.  Odor
problems have been observed to be more severe when the water supply
has a high sulfate concentration.  This observation suggests hydro-
gen sulfide and related sulfur-bearing compounds are important
in the observed odor intensities.

In discussing odors from anaerobic lagoons serving meat packing
plants, J. A. Chittenden (1977)  made the following comments:

     1.  No anaerobic lagoon should be used if the sulfate concen-
         tration of incoming fresh water is in excess of 200 mg/1.
         As a matter of fact, I am reluctant to consider anaerobics
         at sulfate concentrations above 100 mg/1.  The hydrogen sul-
         fide generated by the anaerobic action of concentrations
         above 100 mg/1 produces odors that travel for miles down-
         wind of the treatment system.

     2.  The loading on the anaerobic should range from 15 pounds
         BOD/1,000 ft3 to 25 pounds BOD/1,000 ft3.  Loading signifi-
         cantly out of the range, either higher or lower, results in
         poor anaerobic lagoon performance.  This imposes a high
         load on the subsequent aerobic phases, causing these to
         become septic and generate odors.

     3.  Start-up of the anaerobic lagoon  remains a black
         art.  No one system is quite like another.  There are
         several things to watch and consider.

                               36

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         a.  I see the anaerobic at start-up with primary digester
             sludges from a municipal treatment system.  This helps
             get suspended solids up at start-up and appears
             to give the bugs a headstart.  Forty or fifty thousand
             gallons of sludge is not too much.

         b.  In one case the acid-forming bacteria predominated
             during start-up and the methane-forming bacteria could
             not get started.  As a result, pH dropped to around 5.0
             rather than the near 7.0 pH realized from a well-
             functioning anaerobic lagoon.  Several additions of
             the stoichiometric amount of lime were required to
             overcome the acid formers, but inside of a week the
             methane formers began to function and BOD fell into
             the desired range.

         c.  The sludge cover on the anaerobics forms, in my opinion,
             as a result of the flotation effect from the gas formed
             during anaerobic decomposition.  Sufficient gases are
             not generated until the suspended solids environment
             in the bottom strata of the anaerobics is well-established.
             This takes time—as much as four months in cold weather.
             Bypassing grease recovery systems does nothing to en-
             hance cover formation.  I have had some limited success
             in promoting cover formation by flowing chopped straw on
             the surface of the lagoon.  The straw sinks after three
             or four weeks and must be re-applied, but it does help.

The comments made by Mr. Chittenden typify current odor control tech-
nology.  There has been very little research done having direct
odor control objectives.  Much of the knowledge about odor control
from anaerobic meat packing wastes has been a spin-off from waste
treatment studies.

As indicated earlier, problems result when the following odor pro-
duction steps occur:

     1.  Odorous compounds enter or are formed in the anaerobic
         lagoon.

     2.  Odorous compounds existing in the lagoon volatilize and
         escape to the overhead air.

     3.  Once free to the air over an anaerobic lagoon, the odorous
         gas mixture must be transported downwind to a location where
         it is judged offensive or otherwise undesirable.  This
         transport must occur so the odorous air arrives at the
         point of objection before it has been diluted beyond the
         threshold concentration, oxidized to non-odorous species,
         absorbed by intervening vegetation or structures, or
         reacted with other airborne compounds to attenuate its
         odorous nature.

                               37

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The above odor production steps provide the basis for most odor
control procedures.   Possible odor control alternatives are
outlined as follows:

     1.  Prevent entry or formation of odorous components

             Pretreatment with chemicals to remove or tie
             up odorous precursors - sulfur

             Inhibit  anaerobic decomposition
                  Aeration
                  Sterilization

             Alter environmental conditions to prevent the
             formation of odorous components

     2.  Prevent the  escape of odorous components

             Maintain an effective scum layer

             Install  an impermeable cover

             Chemically treat to prevent volatilization
                  Precipitation
                  pH  adjustment

     3.  Prevent transport of volatilized odorants from lagoon
         to neighbors

             Use of vegetative barriers

             Use of scrubbing screens

             Select lagoon site remote from habitation and
             recreation sites
                               38

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                          SECTION 9

                        RESEARCH NEEDS


Odors from anaerobic lagoons used in the treatment of meat packing
wastes are a significant environmental problem.  In areas where
lagoons are used/ odors present a nuisance problem resulting in
citizen complaints and property damage.  In other locations, an-
aerobic lagoons are rejected in the design process, forcing the
selection of more expensive and energy-intensive processes.

The control of anaerobic lagoon odors offers an opportunity for
technology application with measurable pay-off.  Research tech-
niques have been devised which have the needed sophistication to
make significant contributions.

Among the specific needs are the following:

     Identification of the specific compound with the goal of
     identifying means of eliminating it from water and air.

     Identification of potential chemical treatment processes for
     removing or precipitating odorous components.

     Evaluating means of forming scum layers using synthetic mate-
     rials as well as low-density waste components.

     Measuring the effectiveness of various scum layers in terms
     of thickness and composition.

     Evaluation on a significant size scale of various cover mate-
     rials and fabrication techniques to achieve a minimum cost
     system with appropriate useful life and resistance to physical
     and chemical damage.

     Evaluation of physical and chemical processes involved in
     odorous compound transport with the goal of effectively de-
     signed odor control barriers.
                               39

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                          REFERENCES


Amoore, J. E.  1963.  Stereochemical Theory of Olfaction.  Nature
     198:274.

Amoore, J. E., G. Palmer, and E. Wauke.  1967.  Molecular Shape
     and Odor: Pattern Analysis by PAPA.  Nature 216:1084.

ASTM.  1968.  Manual on Sensory Testing Methods.  American Society
     for Testing and Materials STP No. 434.

Baker, R. A.  1964.  Response Parameters Including Synergism-
     Antagonism in Aqueous Odor Measurements.  Annals of the
     New York Academy of Science 116(2):495.

Earth, C.  1970.   Why Does It Smell So Bad?  Paper No. 70-416.
     American Society of Agricultural Engineers, St. Joseph, MI
     49085.  16 pp.

Burnett, W. E.  1969.  Qualitative Determination of the Odor
     Quality of Chicken Manure.  pp. 2-17.  In: Odors, Gases and
     Particulate Matter from High Density Poultry Management Systems
     as They Relate to Air Pollution.  Final Report, Department of
     Agricultural Engineering, Cornell University.  Contract No.
     C-1101.

Chittenden, J. A.  1977.  Control of Odors from Anaerobic Lagoons
     Treating Meatpacking Wastes,  pp. 38-61.  In: Proceedings,
     Eighth National Symposium on Food Processing.  EPA-600/2-77-184.

Davies, J. T. and F. H. Taylor.  1954.  A Model System for the Ol-
     factory Membrane.  Nature 174:693.

Dean, J. A., ed.   1973.  Lange's Handbook of Chemistry.  llth Edition,
     McGraw-Hill.

Dyson, G. M.  1938.  The Scientific Basis of Odour.  Chem. and Ind.
     57:647.

Gloyna, E. F. and E. Espino.   1969.  Sulfide Production in Waste
     Stabilization Ponds.  In: Proceedings, American Society of
     Civil Engineers.  J. San. Engr. Div. SA3:607.
                               40

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Guadnagni, D. B., R. G. Buttery, S. Okana, and H. K. Burn.  1963.
     Additive Effect of Sub-Threshold Concentrations of Some Or-
     ganic Compounds Associated with Food Aromas.  Nature 200:1288.

Huey, N. A., L. C. Broering, G. A. Jutze, and C. W. Gruber.  1960.
     Objective Odor Pollution Control Investigations.  J. Air Poll.
     Control Assn. 10:441.

Leonardos, G., D. Kendall, and N. Barnard.  1969.  Odor Threshold
     Determinations of 53 Odorant Chemicals.  J. Air. Poll.
     Control Assn. 19(2):91.

Moncrieff, R. W.  1966.  Odour Preferences.  John Wiley, New York.
     p. 89.

Moncrieff, R. W.  1967.  The Chemical Senses.  Leonard Hill, London.
     p. 44.

Moulton, D. G.  1965.  Physiological Aspects of Olfaction.  J. Food
     Science 30:908.

Paulson, D. J.  1967.  Commercial Feedlots—Nuisance, Zoning and
     Regulation.  Washburn Law Journal 6:493-507.

Pomeroy, R. D. and H. Cruse.  1969.  Hydrogen Sulfide Odor Threshold.
     J. Amer. Water Works Assn. 61(12) :677.

Rands/ M. B. and D. E. Cooper.  1966.  Development and Operation of
     a Low Cost Anaerobic Plant for Meat Wastes.  pp. 613-638.  In:
     Proceedings, 21st Purdue Industrial Waste Conference, Lafayette,
     Indiana.

Rosen, A. A., J. B. Peter, and F. M. Middleton.  1962.  Odor Thresh-
     olds of Mixed Organic Chemicals.  J. Water Poll. Control Fed.
     34:7.

Rowe, N. R.  1963.  Odor Control with Activated Charcoal.  Air Poll.
     Control Assn. 13:150.

Sobel, A. T.  1971.  Olfactory Measurement of Animal Manure Odors.
     Proceedings, Agricultural Waste Management and Associated Odor
     Control, Cornell University.  AWM 71-04.

Stahl, W. H., ed.  1973.  Compilation of Odor and Taste Threshold
     Values Data.  ASTM Data Series DS 48, Philadelphia.  pp. 104-
     130.

Waldo, D. A.  1976.  An Evaluation of an Environetics Controliner.
     Unpublished Special Report.  Advisor D. H. Vanderholm, Depart-
     ment of Agricultural Engineering, University of Illinois.
     16 pp.


                               41

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White R. K., E. P. Taiganides, and C. D. Cole.  1971.  Chromato-
     graphic Identification of Malodors from Dairy Animal Waste.
     pp. 110-113.  In: Proceedings, Livestock Waste Management and
     Pollution Abatement.  American Society of Agricultural Engi-
     neers, St. Joseph, MI  49085.  ASAE Publication PROC-271.

Witherow, J. L.  1976.  Waste Treatment for Small Meat and Poultry
     Plants: An Extension Application.  ASAE Paper No. 76-6008.
     Presented at the 1976 Annual Meeting, American Society of
     Agricultural Engineers, June 27-30.  18 pp.

WPCF.  1969.  Safety in Wastewater Works.  Manual of Practice No. I.
     Water Poll. Control Fed.  Washington, DC.

Wright, R. H.  1966.  Why Is An Odour? Nature 209:551.
                                42

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                              APPENDIX

                          RESEARCH PROPOSAL


  One objective of this report preparation was to identify research
  needs in the control of odors from food processing lagoons.   The
  proposal generated  was submitted to the Environmental  Protection
  Agency in September,  1977.

  Title:   Development of a Permeable  Cover to  Control  Odor

  Principal  Investigator:   Dr.  J.  Ronald  Miner, Professor  and Head
                           Department  of  Agricultural  Engineering
                           Oregon  State University
                           Corvallis,  Oregon   97331
                           Telephone:  503  754-2041

 Project Period:  2 years

 Budget: $66,800

 OBJECTIVES OF THIS PROJECT

 This project has as  its overall objective the development of infor-
 mation which will allow the design and construction of  a floating
 permeable cover to be placed on odorous  liquids  to reduce or
 eliminate the escape of odorous gases.   Although the  need for
 and  application of this information is widespread,  it has par-
 ticular application  to odor problems in  the meat processing
 and  livestock production industries.  Anaerobic  lagoons and,
 consequently,  odor problems  are also of  concern  in  fruit  and
 vegetable  processing plants,  dairies, potato  processing plants
 and  rendering facilities.   Permeable  covers have the  potential
 of being  a  satisfactory solution  to  odor problems wherever odor-
 ous  liquids  are  stored in  open  tanks  or  reservoirs.

 Anaerobic  lagoons are  a low cost  technique for the  treatment of or-
 ganic wastewaters.   They  typically achieve 70 to 85 percent BOD
 reduction at a very  low level of  energy  consumption.  Certain  in-
 dustrial and commercial wastes  are particularly  amenable to this
 form of treatment including those discharging a warm water with a
high BOD concentration.  Frequently,  however,  the use of anaerobic
lagoons is rejected in  favor of more  expensive and energy-consump-
tive techniques due to  the inability  to control odor releases.

                               43

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 Odor  problems  are particularly  severe  from  anaerobic  lagoons
 in which  sulfate ion concentration of  the untreated waste is in
 excess of 100  rag/1.

 The overall objective will be approached by pursuit of the first
 three of  the following four procedural steps:

      1.   Develop criteria for a permeable cover to control
          odor  emissions from an anaerobic liquid surface.

      2.   Investigate various materials such as foams, beads,
          natural zeolites and other inert buoyant materials from
          which one might fabricate a floating lagoon  cover.

      3.   Evaluate the cost effectiveness of floating  permeable
          covers as a means of odor control from anaerobic liquid
          surfaces.

      4.   Demonstrate the effectiveness of floating permeable covers
          in an existing odorous lagoon.

 RESULTS AND BENEFITS EXPECTED

 This project is designed to provide a cost effective  technique for
 the control of odors from anaerobic lagoons and other anaerobic
 liquid surfaces.  The only alternatives currently available are
 expensive impermeable covers which encase the entire  lagoon
 surface and require a separate gas treatment scheme or abandonment
 of the anaerobic lagoon process in favor of more expensive and/or
 energy-intensive processes such as aeration basins, aerated
 lagoons,  or automated anaerobic digesters.

 The most  obvious benefit is decreased odor in the proximity of
 anaerobic lagoons; however,  the real benefits are in  the area of
 wastewater treatment.  By making open anaerobic treatment pro-
 cesses environmentally acceptable, the cost of wastewater treat-
 ment is reduced, hence, high levels of BOD removal become econom-
 ically achievable.  Furthermore, since anaerobic lagoons require
 considerably less energy input than alternate forms of treatment,
 development of an effective odor treating cover has a significant
 impact upon the energy consumption of environmental quality
 protection.

 Beyond direct application of these research results to the food
 processing, livestock production and other concentrated organic
waste-generating industries, these research activities are of a
 sufficiently basic nature to have widespread application wherever
 it is desirable to control organic gas escaping from  liquid
 surfaces.   The theory and practices involved will have an impact
on the art of pollution abatement.  Due to the proposed involve-
ment of graduate and undergraduate students, there will also
be measurable educational benefits.

                               44

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APPROACH

The proposed project is consistent with previous work of the prin-
cipal investigator and represents a timely progression of his pre-
vious work.  The attached list of publications reflects the nature
of his previous research relative to odor control technology.
The^timeliness of this work is further supported by current
environmental and social concern over odor and other nuisance
pollutants.

Background

Anaerobic lagoons have been demonstrated to effectively reduce
organic pollutants.  They accomplish this removal with minimum
capital and operating costs.  They are simple to operate, require
no mechanical equipment, and the process can withstand shock load-
ings.  In meat packing plant waste treatment, Chittenden et
al.  (1) report BOD, grease and suspended solids removals in excess
of 80 percent.  Similar removal efficiencies have been reported in
the treatment of other wastewaters.  The major disadvantage of
anaerobic lagoons is the odor resulting from the process, which
can be particularly severe when sulfate concentration in the
water supply is high.  Wherever sulfate concentrations are in
excess of 100 mg/1, odor problems are a subject of concern.
Specific case histories in which odor problems were encountered
from meat processing lagoons were reported by Chittenden et al.
A similar accounting of odor problems from livestock production
was provided by Miner (2).

Costs of using an anaerobic lagoon or aeration were compared
for a 2.88 mgd waste flow meat packing plant in Illinois (1).
The anaerobic-aerobic combination was approximately $1.4 million
less  expensive to build than a completely aerobic system.  A float-
ing flexible membrane to cover with a gas burner was proposed at
an additional cost of $369,000.  Thus, if an effective permeable
cover can be developed at a cost of $3 per square foot or less, a
net initial cost saving can be accomplished.

There is ample evidence in both the literature and common experi-
ence  to support the basic premise of this proposal: namely, that
slow diffusion of odorous gases through either a chemically or
biologically active layer will result in absorption and oxidation
of odorous demonstrations in normal experience, including the
lack  of perceptible odors over buried putrescible wastes or
over  septic tank disposal fields.

The use of soil beds for odor control was reported by Carlson and
Leiser (3).  Their tests indicated that odor reduction was affected
by microorganisms in the soil rather than by ion exchange,  chemical
combination or oxidation.  Moist loam soils were found to have the
greatest odor removal possibilities.  Over a three-month test per-
iod,  hydrogen sulfide gas concentrations of 15 ppm at a flow rate

                               45

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 of  0.35  ft /min per square foot of soil surface were reduced to an
 imperceptible level in 32 inches of soil.  For a  flow rate of 0.34
 ft  /min  per square foot in a hydrogen sulfide concentration of 9.5
 ppm, 90  percent of the hydrogen sulfide was removed in the first
 18  inches of soil.  Effectiveness of soil beds in removing hy-
 drogen sulfide did not diminish during a three-month test period.
 A soil filter for the removal of odors from a Mercer Island,
 Washington, pumping station has been in successful operation for
 several  years.

 As  an outgrowth of the earlier work, Carlson and  Gumerman  (4) pro-
 posed a  system for the treatment of odorous gases.  Their system
 included a perforated tile system through which odorous gases
 were blown.  Above the tile system, the soil was  covered with
 a greenhouse to facilitate year-round plant growth.  The role
 of  the plants within the system was to keep the structure open,
 to  utilize some of the excess sulfur, and to replenish the soil
 organic matter sacrificed in the active biological growth that
 occurred.  They suggested a plant with a shallow  root system which
would meet the above goals but not interfere with the gas dis-
 tribution piping system.

 The use of soil filters for the removal of animal waste odors was
 investigated by Burnett and Dondero (5).  They based their initial
 trials on the earlier work of Carlson and Leiser  (3)  and Gumerman
 and Carlson (6)  and found that, indeed, the use of soil columns
was effective in removing both hydrogen sulfide and ammonia from
 the headspace gas over decomposing poultry manure.  They found that
 for ammonia concentrations of up to 200 ppm, removals of 100
percent were obtained, and for hydrogen sulfide concentrations
of  22 to 100 ppm, more than 95 percent removal occurred throughout
a three-month continuous testing period.  They further found
 that when the soil columns dried,  ammonia removal efficiency
dropped rapidly.  Thus, to be fully effective, the moisture
content of the soil must be maintained.  By mixing manure with
 soil prior to using it in the column,  the moisture-holding capa-
city was increased.  As a result of this work, they made some
 tentative suggestions as to the area required for odor removal.
Assuming a 40,000-layer operation,  they suggested that a trench
two feet deep,  two feet wide and 903 feet long would be required
to deodorize the air.   This is equivalent to 0.0903 cubic feet
of soil per bird.

REFERENCES

1.   Chittenden, J. A., L.  E.  Orsi, and J.  L.  Witherow.   Control of
     Odors from an Anaerobic Lagoon Treating Meat Packing Wastes.
     Proceedings/ Eighth National  Symposium on Food Processing
     Wastes.   Seattle, Washington.   1977.

2.   Miner,  J.  R.  Odors From Confined Livestock Production.
     Environmental Protection Technology Series.   EPA-660/2-74-023.
     125 pp.   1976.
                               46

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3.   Carlson, D. A. and C. P. Leister.  Soil Beds for the Control
     of Sewage Odors.  J. Water Poll. Control Fed. 34:829-840.
     1966.

4.   Carlson, D. A. and R. C. Gumerman.  Hydrogen Sulfide and
     Methyl Mercaptans Removals with Soil Columns.  Proceedings,
     21st Industrial Waste Conference.  Purdue University, Lafay-
     ette,  Indiana.  1966.

5.   Burnett, W. E. and N. C. Dondero.  The Control of Air Pollu-
     tion (Odors) from Animal Wastes - Evaluation of Commercial
     Odor Control Products by an Organoleptic Test.  Paper No.
     68-909. American Society of Agricultural Engineers.  De-
     cember, 1968.

6.   Gumerman, R. C. and D. A. Carlson.  Chemical Aspects of Odor
     Removal in Some Soil Systems.  pp. 292-302.  In: Animal Waste
     Management.  Cornell University Conference on Agricultural
     Waste Management.  1969.

WORK PLAN

The first step in this project is evaluation of the basic process
which will be accomplished by fabricating circular pads of dense
fiberglass filter materials ranging in thickness from one to
six inches.   They will be fabricated into a circular configuration
to fit snugly inside the plastic lined 55-gallon drums proposed
for simulating the lagooning process.  Twelve barrels will be
used initially.  They will be filled with cattle manure for the
first trial, then with a simulated meat packing plant waste for
the second.   For both trials, one-third of the barrels will be
spiked with sulfates to provide a net sulfate ion concentration of
100 mg/1 and an equal number to 300 mg/1.  Effectiveness will be
evaluated by measuring the ammonia and hydrogen sulfide evolution
rates and by scentometer evaluations made at the rim of the barrel.

Based upon the results of the initial trials, alternate techniques
of fabricating permeable lagoon covers will be evaluated.  The
materials discussed in the detailed objectives will be included.
The goal during this phase of the effort will be to identify those
basic physical characteristics which define the effectiveness of a
lagoon cover; however, there is likely an additional factor re-
lating to the distance of travel by the gas phase.  Once these
questions are resolved, covers made of different materials with
standard levels of odor abatement can be defined.  From this
point, it is possible to calculate the cost effectiveness of
alternate designs.  The next ingredient is the long term use-
fulness of specific covers, which will be determined using the
model lagoons described above.  The schedule for accomplishing
the various tasks is shown in Figure 1.
                                47

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                      FIGURE A-l.  TIME SCHEDULE FOR PROGRESS OF THE PROJECT
                                          First year (quarters)   Second year  (quarters]
1
2
3
4
1
2
3
4
   Assemble personnel & equipment



   Train staff & design initial covers



   Evaluate standard covers

*»
00
   Prepare first year report



   Design alternate covers



   Evaluate alternate covers



   Identify site for field test



   Prepare design for field test



   Prepare final report

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REVIEWS

The proposal was evaluated by six technical reviewers who have
extensive experience on the subject.  A summary of this review
follows.

Mr. //// /. ///////// of the Environmental Protection Agency, aided
the development of this proposal over the year and had previous-
ly developed a research proposal with similar, but less extensive,
objectives.  He finds the overall project design, available re-
sources,  project period, and budget satisfactory.  He states the
principal investigator's background and experience are ideal.  His
concerns are the brevity of the design and the developmental nature
of the project.  This development requires exploration for more
effective material for odor reduction.

Dr. /. /. ///, Agricultural Engineering Professor at the /////////
// ///////// was selected at random to review the proposal.  He
states that the project's objectives are of interest to the EPA
mission and that the project could contribute significantly in
control of odor and use of low cost waste treatment.  He knows
of no overlap of the proposed project with other work, but men-
tioned that some research on alternate control techniques has been
undertaken.  He states that the project period, resources, and
facilities are adequate, and that the principal investigator
has the needed background and training and has a long successful
record of research in the area.  Dr. /// makes two suggestions:
(1) the model lagoon should be operated through a range of ex-
pected temperatures for expected seasonal variations, and  (2)
continuous loading and discharge of waste be used in the exper-
iment to more nearly simulate actual conditions.

Dr. //// /. ///////, Dean, College of Engineering, ////////// //
/////////// was asked to review the project because of his research
in odor control using soil systems.  Dr. /////// accurately des-
cribes the objectives and recommends the area of work.  He states,
"Not a great deal of work has been done in the area proposed."  He
describes the budget as sufficient to get the project under way and
states a second stage (year) of work should be undertaken if pro-
gress shows worthwhile results.  He further states a third stage of
field evaluation is needed if the developed system is feasible.
Dr. /////// writes the background information is reasonable, but he
would like more details on the proposed work.  He later states such
project details could not be expected until the principal investi-
gator has funds sufficient to allocate time to the project.  He
has several specific concerns or suggestions.  These are:  (1)
use of 55-gallon drums for digesters will cause problems "because
of iron, temperature, current, and depth perturbations";  (2) the
development of a permeable cover should include more materials of
a biologically active or amenable base; the possibility for a joint
soil-glass fiber system or a styrofoam log with soil interface sys-
tem for breathing of odorous gases is suggested.

                               49

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Dr. /// ///////, Extension Agricultural Engineer, ///// ////////////
///////// ///////, was selected to review the proposal because of
his publications and experience in control of odors from anaerobic
lagoons and feedlots.  Dr. /////// strongly recommends that EPA
fund this grant, which will provide significant information on the
control of odors.  He noted that in his state, 45 percent of all
air pollution complaints involve odors, and that anaerobic lagoons
are an efficient and cheap method of waste treatment, but are often
the leading cause of odors found around livestock feeding opera-
tions.  Dr. /////// writes, "No organized research has yet dealt
with the use of permeable covers as a means of odor abatement."  He
thinks "a permeable floating cover for lagoons is a unique approach
widely applicable to thousands of existing feeding operations."
He states the principal investigator is one of the best qualified
researchers in the U. S. to conduct an investigation of the type
proposed.  He feels the two-year project period and the budget are
appropriate, finds no major weakness with the proposal, and lists
as one of its strong points the use of plastic-lined 55-gallon
drums to simulate lagooning.

Mr. /. /. //////////, Vice-President for Research, /////, has been
responsible for design of a number of meat packing waste treatment
systems for a large meat packer.  He writes that the project is
sound and the benefits to EPA appreciable.  He describes anaerobic
lagoon operations and lists three specific benefits of the project
to these operations: (1) it would allow the use of anaerobic la-
goons treating waters with sulfate concentrations above 100 mg/1;
(2) it would be beneficial during the start-up period of an an-
aerobic lagoon when odorous emissions are commonly experienced
regardless of sulfate concentrations;  (3) it would be useful in
case of intermittent loading to the anaerobic lagoon due to sea-
sonal operation of the plant (common in the food industry).
The benefits would be not only to control odor by maintaining
a cover during plant shutdowns, but also during re-introduction
of waste.  He knows of no similar work being conducted, except
on impermeable covers (which he describes as too costly, except
in the case of very high sulfate waters).  He thinks the staff,
support facilities, project period, and project budget are ade-
quate.  The strengths of the project are the use of 55-gallon
drums as digesters, the use of 12 digesters, and the range of
sulfate concentrations at 1, 100, and 300 mg/1.  The weakness
of the project is finding an acceptable medium to act as a per-
meable cover.  He suggests that insulated thermostatically con-
trolled barrels  (drums)  be used to maintain constant temperature
and constant gas production.  He states that even if the project
fails to produce an acceptable cover material, demonstrating
a relationship between odor emissions and surface area per unit
of cover would justify this study.
                               50

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Mr. /. /. //////, Research Chemist with the //////// ///////
//////// /////////// ///., New Zealand, was requested to review
the project because of his two publications on the subject.
////// repeats the objectives without describing their benefits.
His seven page review includes description of his research and ob-
servations, as well as that of others, on the subject.  The more
pertinent points follow.  The length of operation necessary to form
a scum crust and become odor-free varies between 4 and 12 months.
These crusts may reach one to three feet in thickness,  and at
one anaerobic lagoon, cleaning was necessary every six months
to a year.  (These conditions usually occur when paunch manure
is discharged to the lagoon). At another anaerobic lagoon, a
1/4- to 1/2-inch crust was maintained for odor control and acted
as a biological filter with action due partly to iron bacteria
in symbiotic association with red and green photosynthetic sulfur
bacteria.  He states that the proposed use of a cheap porous
material may be necessary to form a satisfactory scum mat for
odor control and suggests the addition of cattle and sheep paunch
material  (a practice strongly opposed by EPA and cost factors
since the presence of BPT and BAT discharge limitations).

Mr. ////// writes the proposal gives a well-rounded approach and
that the materials listed are worth investigating.  He ranks the
principal investigator as high caliber, and the organization and
support facilities as ideal.  The project period and budget are
adequate, but he suggests the project time could be longer.  He
considers the central theme a valid one and recommends selecting
materials for the cover that can be easily removed and replaced.
He continues saying that on large lagoons when sludge buildup or
maintenance is needed, a dragline is a simple means of solids
removal, and for this reason a non-rigid cover material would be
better.  He concludes that use of 55-gallon drums is sound and
generation of a fibrous cover can stop odors.
                               51

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                                   TECHNICAL REPORT DATA
                                rcaJ iii^trui'tions on the reverse before completing)
 1. R I PORT NO.

 EPA-600/2-78-151
 4. Fl T Lfc AND SUBTi TLE
  Control  of Odors from Anaerobic  Lagoons Treating
  Food Processing Wastewaters
                                         6. PERFORMING ORGANIZATION CODE
 7. AUTHOR(o)
  J.  Ronald Miner
                                                           8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
 Agricultural  Engineering Department
 Oregon State University
 Corvallis, Oregon  97331
 12. SPONSORING AGtNCY NAMt AND ADDRESS
  Industrial Environmental  Research Laboratory
  Office of Research and Development
  U.S.  Environmental Protection  Agency
  Cincinnati, Ohio 45268
                                                           3. RECIPIfc'NT'S ACCESSION-NO.
                                         5 REPORT DATE
                                          July 1978 issuing date
                                                           10. PROGRAM ELEMENT NO.
                                                 1AB60U
                                         11. CONTRACT/GRANT NO.
                                          CC691935-J
                                         13. TYPE OF REPORT AND PERIOD COVERED
                                          7/1/76  - 12/31/76    Finai
                                         14. SPONSORING AGENCY CODE

                                                 EPA/600/12
 15, SUPPLEMENTARY NOTFS
  Project Officer:   Jack L. Witherow,  Food  and  Wood Products Branch--Corvallis,  Oregon
 16. ABSTRACT
 Anaerobic lagoons are used  for  the treatment of meat packing  wastes in most area of
 the country.  They are  a  relatively low cost means of achieving  BOD reduction.
 Although lagoon effluent  is  not suitable for stream discharge,  it is amenable to
 further treatment or to land application.   One of the most  serious limitations of
 anaerobic lagoons in this application is odor production.   Odor  complains have
 been widespread but have  been most frequent in areas of  high  sulfate waters and
 during start-up.  There has  been little specific research effort devoted to anaerobic
 lagoon odor control.  This  report assembles existing information relative to odor
 control associated with anaerobic lagoons  used in the meat  packing industry and
 identifies opportunities  for productive research.  It provides a basis for approaching
 the overall problem in  a  comprehensive fashion.  This report  was submitted in
 fulfillment of Contract No.  CC691935-J by the Agricultural  Research Foundation of
 Oregon State University under the sponsorship of the U.  S.  Environmental Protection
 Agency.  It covers the  period July 1, 1976 to December 31,  1976, and work was
 completed as of December  31, 1976.
 Odors
              KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTOR:;                   h.IDENTIFIERS/OPEN ENDED TERMS

                             Anaerobic Lagoon
                             Meatpacking
                             Food Processing  Industry
                            Anaerobic  Digestion
                            Wastewater  Treatment
   [Jl.j F RIHU riON STATEMENT


             RELEASE TO PUBLIC
                            19 ^FCURITY CLASS ( This Report)
                               UNCLASSIFIEJD	
                            20 SECURITY CLASS (Hits page I

                               UNCLASSIFIED
                                                                        c.  COSATI I ifld/Oroiip
                                                                             13B
                                                                        21. NO. OF PAGES
  60
22. PRICE
EPA Form 2220-1 (9-73)
                                                   •/, U. S. GOVERNMENT PRINTING OFFICE: 1978-757-140/1369 Region No. 5-||

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