&EFA
            United States
            Environmental Protection
            Agency
           Robert S Kerr Environmental Research EPA-600/2-80-125
           Laboratory          May 1980
           Ada OK 74820
                     REGION III
            Research and Development
                                         BGTIUH '
Cyanide Removal from
Refinery Wastewater
Using Powdered
Activated Carbon

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                RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology. Elimination of traditional grouping was consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:

      1.  Environmental Health  Effects Research
      2.  Environmental Protection Technology
      3.  Ecological Research
      4.  Environmental Monitoring
      5.  Socioeconomic Environmental Studies
      6.  Scientific and Technical Assessment Reports (STAR)
      7.  Interagency Energy-Environment Research and Development
      8.  "Special" Reports
     9.  Miscellaneous Reports

This report has  been assigned  to the ENVIRONMENTAL PROTECTION TECH-
NOLOGY series. This series describes research performed to develop and dem-
onstrate instrumentation, equipment, and methodology to repair or prevent en-
vironmental degradation from point and non-point sources of pollution. This work
provides the new or improved technology required for the control and treatment
of pollution sources to meet environmental quality standards.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                             EPA-600/2-80-125
                                             May 1980
     CYANIDE REMOVAL FROM REFINERY UASTEUATER
          USING POWDERED ACTIVATED CARBON
                   James E. Huff
                 Jeffrey M. Bigger
             IIT Research Institute
             Chicago, Illinois 60616
             EPA Grant No. R804029-01
               IITRI Project C6358
                  Project Officer
               Thomas E. Short, Jr.
             Source Management Branch
 Robert S. Kerr Environmental Research Laboratory
             Ada, Oklahoma 74820
            This study was conducted
            1n cooperation with the
  Illinois Institute for Environmental Quality
            Chicago, Illinois  60606
ROBERT S. KERR ENVIRONMENTAL RESEARCH LABORATORY
       OFFICE OF RESEARCH AND DEVELOPMENT
      U.S. ENVIRONMENTAL PROTECTION AGENCY
               ADA, OKLAHOMA 74820

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                                 DISCLAIMER
     This report has been reviewed by the Robert S. Kerr Environmental Re-
search Laboratory, U.S. Environmental Protection Agency, and approved for
publication.  Approval does not signify that the contents necessarily re-
flect the views and policies of the U.S. Environmental Protection Agency,
nor does mention of trade names or commercial products constitute endorse-
ment or recommendation for use.
                                      11

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                                   FOREWORD
     The Environmental Protection Agency was established to coordinate adminis-
tration of the major Federal programs designed to protect the quality of our
environment .

     An important part of the Agency's effort involves the search for informa-
tion about environmental problems, management techniques and new technologies
through which optimum use of the Nation' a land and water resources can be
assured and the threat pollution poses to the welfare of the American people
can be minimized.  EPA's Office of Research and Development conducts this
search through a nationwide network of research facilities.

     As one of these facilities, the Robert S. Kerr Environmental Research
Laboratory is responsible for the management of programs to: (a) investigate
the nature, transport, fate and management of pollutants in ground water;
(b) develop and demonstrate methods for treating wastewaters with soil and
other natural systems; (c) develop and demonstrate pollution control technol-
ogies for irrigation return flows; (d) develop and demonstrate pollution con-
trol technologies for animal production wastes; (e) develop and demonstrate
technologies to prevent, control, or abate pollution from the petroleum refin-
ing and petrochemical industries; and (f) develop and demonstrate technologies
to manage pollution resulting from combinations of industrial wastewaters or
industrial/municipal wastewaters.

     Various effluent standards typically require that cyanide levels be kept
below concentrations in the microgram-per-liter range.  Such levels are diffi-
cult to achieve in industrial operations like petroleum refining because of
daily and seasonal fluctuations in raw waste content.  This report contains
data on a promising treatment technology for effecting significant cyanide re-
ductions with minimal capital investment.  Development of such technology is
essential if EPA is to establish and enforce pollution control standards which
are reasonable, cost-effective, and which provide protection for the American
public.
                                         W. C. Galegar
                                           Director
                       Robert S. Kerr Environmental Research Laboratory
                                      iii

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                                  ABSTRACT

     This research program was initiated to investigate the feasibility of
utilizing powdered activated carbon (PAC) and cupric chloride (CuCl2) for
removal of cyanide in refinery wastewaters.

     A two-phase program, consisting of batch tests and continuous tests,
was conducted to determine the basic chemistry and cyanide removal efficiency
of the adsorption and catalytic oxidation of cyanide by PAC, and CuCl2.  In
the first phase the operating variables of pH, copper dosage, mode of copper
addition, carbon dosage, and type of carbon were investigated.  The results
indicated a pH near neutral (pH 6-8.5) was desirable to obtain low equilibri-
um cyanide in the aqueous phase while maintaining a low copper level.  Cyanide
removal, greater than 95%, was readily achieved in the batch tests using 250
mg/H of powdered carbon and 1.0 to 1.5 mg/Jl of copper on solutions containing
0.5 mg/t iron cyanide.  The most important factors in cyanide removal were
the copper concentration and carbon concentration in the solution.

     Phase II was a series of continuous tests using two laboratory scale
activated sludge units and actual refinery wastewater.  Both carbon and cop-
per were added to the aeration basin, and the organic removal as well as
cyanide removal performance was monitored.  The results of these continuous
tests revealed that cyanide can be successfully removed through the addition
of PAC and CuCl2 into an activated sludge unit.  The biological efficiency
did not indicate any detrimental effects from the copper addition (with the
exception of the first test where the aeration basin was slug-dosed).   More
carbon is required than predicted in the batch tests due to the organics
competing with cyanide for the active sites on the carbon.

     An economic evaluation indicated that this process requires little or no
capital expenditure and should provide many refineries with an economic ap-
proach for reducing effluent cyanide concentrations.

     This program was made possible by the co-sponsorship of the U.S. Environ-
mental Protection Agency and the Illinois Institute for Environmental Quality.
This report covers a period from September, 1975 to February, 1977, and the
actual laboratory studies were completed in July, 1976.
                                     IV

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                                CONTENTS
                                                                   Page
Foreword	i in-
Abstract  	jv
Figures	vi
Tables	vii
List of Abbreviations and Symbols	viii

     1.   Introduction	   1
     2.   Conclusions & Recommendations	! ! !  !   2
     3.   Background Information	   4
              Cyanide generation in petroleum refineries  	   4
              State and Federal regulations regarding cyanide. .  .  12
              Existing cyanide treatment methods 	  13
              Adsorption with catalytic oxidation	14
              Potential application of adsorption with catalytic
                 oxidation to refinery cyanide wastewater	17
     4.   Experimental Systems and Analytical Methods 	  21
              Experimental set up	21
              Sampling procedure 	  25
              Analytical methods 	  27
     5.   Phase I - Experimental Results	30
              Description & results of batch tests 	  30
              Copper toxicity	33
              Regression analysis	39
              Oxidation of cyanide adsorbed on the carbon	43
              Conclusions from Phase I	45
     6.   Phase II - Experimental Results	46
              Test A - Control  test	47
              Test B - Low carbon/low copper test	49
              Test C - High carbon/low carbon test	52
              Test D - High carbon/High copper test	57
              Test E - Lower organic loading with medium carbon/
                       high copper dosage	74
              Summary of Phase II	77
     7.   Economic Implications of the PAC/CuCl2 System 	  80
              Operating criteria for PAC/CuCl2  systems 	  80
              Cost estimation of PAC/CuCl2  systems 	  82
              Cost comparison of PAC vs 6AC cyanide removal
                 systems	82

References	38
Appendices	90
Glossary of Terms	97

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                                   FIGURES

Number                                                                   Page

   1  Cyanide generation and disposal  in refinery 	   5
   2  Refinery A - Effluent total  cyanide distribution	7
   3  Refinery B - Effluent total  cyanide distribution	8
   4  Refinery A - Monthly average cyanide discharged  	   9
   5  Refinery B - Monthly average cyanide discharged  	  1°
   6  Iron cyanide disappearance  rate	16
   7  Experimental reactor	22
   8  Single stage activated sludge system	24
   9  Cyanide distillation apparatus	29
  10  pH effect on rate of cyanide removal	31
  11  Equilibrium cyanide levels  as a  function  of copper dose  	  35
  12  Equilibrium cyanide levels  as a  function  of carbon concentration.  .  36
  13  Test C - Predicted effect of PAC on effluent cyanide  levels  ....  58
  14  Test D - Cyanide  frequency  distributions	60
  15  Effect of powdered carbon on BOD5 removal  - Test  D	61
  16  Test D - Effluent cyanide levels with  time	62
  17  Test D - Inlet  cyanide level  with time	63
  18  Test D - Cyanide  frequency  distributions  - By period.  	  67
  19  Test D - Effluent BOD5 with  time	68
  20  Test D - Inlet  BOD5 with time	69
  21  Test D - Inlet  and effluent  TOC  with time	70
  22  Test E - Effluent cyanide levels with  time.	75
  23  Test E - Effluent cyanide frequency distribution	78
  24  Effect of carbon  dose on apparent loading 	  81
                                     Vi

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                                   TABLES

Number                                                                  Page

   1  Summary of Refinery Cyanide Levels 	 ^
   2  Summary of State Standards for Cyanide 	 IJ
   3  Results of pH Effect on Ferricyanide Removal 	 32
   4  Filtrate Copper Levels, mg/fc 	 32
   5  Mode of Copper Addition, 1.5 mg/fc Initial Copper Dose	33
   6  Mode of Copper Addition, 0.5 mg/A Initial Copper Dose	33
   7  Effect of Carbon Type on Ferricyanide Removal	3Z
   8  Filtrate Copper Levels 	 38
   9  Summary of Regression Analyses 	 41
  10  Batch Tests - Cyanide Decay Results	44
  11  Test A - Summary Results	48
  12  Test A - Statistical Results	50
  13  Test B - Summary Results	51
  14  Test B - Statistical Results	53
  15  Test C - Summary Table	55
  16  Test C - Statistical Results	5$
  17  Test D - Summary Table	64
  18  Test D - By Periods - Summary Table	65
  19  Test D - Statistical Results	71
  20  Cyanide Removal Across the Control Reactor  	 72
  21  Apparent Carbon Loadings  	 73
  22  Test E - Summary Table	76
  23  Chemical Cost Summary	83
  24  Cyanide Treatment  - Chemical Cost  Comparison 	 85
  25  Cost Estimates  for PAC/CuCl2 System	86
                                     Vli

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                      LIST OF ABBREVIATIONS AND SYMBOLS
ABBREVIATIONS

API
BOD
COD
GAC
6PM
MGD
ML
MLSS
PAC
PPM
R2
SS
TOC
TSS
American Petroleum Institute
bi ochemi cal oxygen demand
chemical oxygen demand
granular activated carbon
gallons per minute
million gallons per day
milligrams per liter
mixed liquor
mixed liquor suspended solids
powdered activated carbon
parts per million
coefficient of determination for linear regression
suspended solids
total organic carbon
total suspended solids
SYMBOLS

CN-
CuCl2
H2S
ftl
n
s
t
Yi
cyanide
cupric chloride
hydrogen sulfide
expected difference in population means
unbiased standard deviation of MD
mean difference
degrees of freedom
sample size
standard deviation
't1 statistic with n-1 degrees of freedom
observed value of the i th sample
                                     viii

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                                  SECTION 1

                                INTRODUCTION


     The operation of nearly all modern petroleum refineries results in the
discharge of cyanides to the receiving streams.   The concentration of refin-
eries and other cyanide discharging industries along selected waterways com-
pounds the potential toxic effects in certain waterways.  Various cyanide
water quality standards from 0.005 mg/5, to 0.2 mg/£ have been proposed or
adopted by state and federal agencies.  A few state agencies have also adopted
effluent standards of 0.025 and 0.05 mg/X, total  cyanide to assist in maintain-
ing water quality.

     The formation of cyanide in an inherent result in the production of gas-
oline components where the catalytic cracking process is employed.  The
cyanide produced in the cracking process ends up in the "sour water" system which-
is sent to a steam or natural gas stripping column.  Some of the cyanide is
removed in the stripper, while the remaining cyanide wastewater is eventually
discharged to the sewer.  Effluent cyanide levels from 0.020 mg/£ to 0.2 mg/£
are typical in the industry.  Large fluctuations, both in the daily and sea-
sonal cyanide levels, makes control of the problem that much more difficult.

     Other than the stripping operation and maximizing water reuse, only
minimal cyanide treatment is presently practiced in the refining industry.
Existing treatment methods for cyanide are characterized by energy-intensive
and expensive processes.  A potential low-cost cyanide treatment method for
petroleum refinery wastewaters was evaluated in this study.  The method in-
vestigated can be classified as an adsorption/oxidation process and utilizes
powdered activated carbon, copper salts, and dissolved oxygen.  The potential
application point selected for this study was the biological activated sludge
units common in the refining industry for secondary wastewater treatment.
Utilization of the activated sludge units minimizes the capital investment
requirements and also provides biological regeneration of the carbon, thus
minimizing the operating expenses.

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                                   SECTION 2

                      CONCLUSIONS AND RECOMMENDATIONS

     1.  In the absence of dissolved organic material, inlet cyanide concen-
trations of 0.5 mg/fc can be reduced to below 0.025 mg/fc with the addition of
powdered activated carbon (PAC), cupric chloride (CuCl2)» and dissolved oxygen.

     2.  The presence of cupric ions in solution greatly enhances cyanide
adsorption.  As demonstrated in the batch tests, overall cyanide removal in-
creased from 65% to 99% when the copper level was raised from 0.5 mg/JL to
1.5 mg/JL

     3.  The cyanide level  in refinery effluents can be reduced from current
levels through the use of PAC and CuCl2.

     4.  For most refineries the most economical location of a PAC/CuCl2
process is the refinery wastewater activated sludge units.

     5.  When there is a high organic loading in the wastewater, competition
for the adsorption sites occurs between the organics and the cyanide.  As the
organic quantity increases, the amount of carbon required becomes greater.
The most economically favorable location for a PAC/CuCl2 system is the second
stage of a biological system or in a stream of low organic loading.

     6.  The economics of PAC/CuCl2 systems vary according to refinery flow,
cyanide level, and organic loading.  For example, the costs estimated for four
refineries with single-stage activated sludge units range from $46,000 to
$500,000 per year.  The location of this cyanide removal process in the
second stage of an activated sludge unit could reduce the costs to $20,000 to
$150,000 per year for the same refineries.  Clearly, the second stage, where
available, is a more economical location for the PAC/CuCl2 process.

     7.  For refineries that currently employ the activated sludge process,
little or no capital expenditure is required to use PAC/CuCl2 for cyanide
removal.  Thus the flexibility of this process is enhanced.  PAC/CuCl2 can be
used for short term cyanide peaks in the effluent attributed to refinery turn-
arounds or operating changes in the catalytic cracking unit.

     8.  The experimental results indicate that cyanide removal is possible
with a PAC/CuCl2 process; however, the effectiveness of long term treatment
is uncertain at this time.   The effect of fluctuating organic loadings and
the stability of the treatment system over an extended time period need to
be better defined for satisfactory application of this process.  Sufficient

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data have been gathered to develop a full scale study  in a refinery for deli-
neation of PAC's operational feasibility.  Especially  since no capital invest-
ment is required, the success of the PAC/CuCl2 process as a practical tool for
refineries can be readily demonstrated and should be considered as an impor-
tant sequel to this study.

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                                   SECTION 3

                                   BACKGROUND


     Before presenting the results from this study, background information
related to the cyanide problem is provided.  In this section a description of
the source of cyanide in refineries and existing state and federal  laws is
included, along with the potential advantages and limitations of the proposed
treatment scheme.  This background material delineates the need for an econ-
omical cyanide treatment method and the potential of the treatment scheme is
investigated in this study.

CYANIDE GENERATION IN PETROLEUM REFINERIES

     Cyanides are present in the effluents of nearly all modern petroleum
refineries in the United States'.  The principal source of cyanide in a refin-
ery is the catalytic cracking unit, with some contribution from the coking
unit.  Over 92% of the U.S. refineries utilize the cracking process to break
heavy petroleum fractions into principally gasoline components.  Two types of
cracking units are common in the industry, thermal and catalytic.  As the name
implies, thermal  cracking requires high temperatures (900-1100°F) and high
pressures (600-1000 psig) to break down the heavy petroleum fractions.  Cata-
lytic cracking requires lower temperatures and pressures.  The most common
catalytic process is the fluid catalytic cracker (FCC) unit, which contains
a powdered catalyst.

     The trend during the last decade has been toward larger fluid catalytic
cracking units because of the higher gasoline yields produced.  Over 57% of
the refineries in the United States use a catalytic cracking unit to meet the
high demand for gasoline (relative to fuel oils).  Cyanide is a major pollu-
tant in the wastewaters from refineries employing cracking units, especially
the catalytic cracking units.

     Presented in Figure 1, is the path the cyanide waste stream follows in a
"typical" refinery.  As the heavy feedstocks are cracked in the reactor sec-
tion, many different compounds are formed.  A large amount of nitrogen is
liberated in the form of ammonia; 'however, what appears to be a small amount
of cyanide is also generated, typically 5 to 100 ppm.  From the reactor cham-
ber, all cracked material goes overhead as a gas to a distillation column,
where the cyanide and ammonia are distilled off the top of the column with
lower molecular weight organics, (butane and lighter).  Because of the ex-
tremely corrosive nature of the cyanide gas, water is injected in the over-
head gas line from the fractionating column.  This water is collected in one
or two accumulators or knockout drums.

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                                              H20
               Reactor
    Catalyst
     Recycle
       Rgntr
                                                        I

                                     Cooler
       V-U
                                                            Vapors to
                                                            Gas Plant
                                                          Accumulator
r
                          Fractionating   I
I  Raw Oil  __^	[           Column      I
'  Charge                                '
I                                        I
j	   Fluid Catalytic Cracker	|
Crude
 Oil
                       I
                 	I

                 Sour Water
                                            Sour Water
                                            from Other
                                              Units
            Sour Water Stripper Bottoms
Desalter


   To
 Sewer
                                                     >*-
                    Crude
                     Oil
                           To
                          Sewer
                                                                        Recycle to
                                                                         Units for
                                                                         V.'ashwater
                                                                                         To
                                                                                     Sulfur Plant
                                                                                     Incinerator
                                           Sour  Water
                                            Stripper
                       Figure 1.   Cyanide  generation and disposal in refinery.

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     The accumulator water contains high levels of cyanide (20-200 mg/£),
phenols (500-2000 mg/£), sulfides (1,000-10,000 mgA),  and ammonia (1,000-
10,000 mg/fc).  This water, often termed "sour-water," is pumped to a stripping
column along with sour water from other process units.   The purpose of the
sour water stripper is to remove a large portion of the pollutant load prior
to discharging to the sewer.  Most strippers use steam  or natural gas as the
stripping agent and send the overhead gases to an incinerator.   Strippers are
typically designed to remove 99% of the H2S, and will also remove 90-98% of
the ammonia and 30-70% of the phenols.  The amount of cyanide removed ranges
from 20 to 80%, depending upon the type of cyanide present and  the inlet cya-
nide concentration.  A 1972 American Petroleum Institute study  on sour water
strippers reported an industry average cyanide removal  of 37% by stripping.1
Changes in the pH, stripping rate, and other variables, have little effect on
the cyanide removal efficiency, suggesting the remaining cyanide has complexed
with some heavy metals.

     From the bottom of the stripper, the water can go  several  places, depen-
ding upon the practices of the individual refineries.  Many refineries return
part of the stripped sour water or stripper bottoms to  the cracking and coker
units.  The stripped sour water is also commonly used for desalting of the
crude oil and then discharged to the sewer.  Another potential  practice for
some refineries is to send the stripped sour water to the cooling towers.
Where reliable stripper operation exists and the trace  sulfides do not inter-
fere with the corrosion inhibitors in the cooling towers, recycling the strip-
per bottoms to the cooling towers is an excellent water conservation practice.
However, most of the cyanide found in the stripped sour water eventually finds
its way to the sewer regardless of the reuse practice of the individual refin-
eries.

     One of the major problems in controlling cyanide from refineries is the
large variability in the cyanide levels generated.  This variability is not
only a day-to-day phenomena, but also a month-to-month  problem.  Shown in
Figures 2 and'3 are daily cyanide frequency distribution curves for two mod-
ern refineries in Illinois.  The change in the monthly  average cyanide for
these same two refineries is presented in Figures 4 and 5.  The daily cyanide
distribution curves are very similar, each showing a large number of occur-
rences between 0.000 to 0.050 mq/i and a long "tail" or skewed  distribution
beyond 0.25 mg/A.  Thus, where standards based on daily maximum levels are
utilized, any cyanide treatment methods must be capable of handling wide
fluctuations in inlet cyanide concentrations.  Figures  4 and 5, indicate that
any cyanide treatment system must also be designed to handle extended periods
of high cyanide loadings.

     Typical refinery cyanide effluent levels are presented in Table 1.  The
range of cyanide levels for the eight refineries included in Table 1 goes from
0.005 mg/fc to 0.176 mg/£ for the mean cyanide discharged.  The maximum levels
discharged vary from 0.03 mg/fc to 0.730 mg/fc total cyanide.   While cyanide is
present in all eight refineries in Table 1, the magnitude of the effluent
cyanide concentrations varies by a factor of 30 from the lowest to the high-
est discharger.

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    56  r
u


il
U
o
    32 •
    16 -•
     8 -•

/
/
1
1
-









^ """* -.










\
\
\
\
1
1
1
1
Total H»6 observations
1 from 7/1 /7^ - 8/29/75
, 2 observations > 0.300
| mg/£
1 Mean value = 0.07 rng/i
\ U.S. EPA test method
1 2^-hr composite samples
i ... 	
l
I
\
V
\
\
N
X
x
**.
^ ._
^-
*^
"^^^^_
	 1 	 ! 	 1 	 1 	 »-
           0.025   0.05
 0.10         0.15          0.20

Total Cvanide Concentration, mg
                                                                        0.25
                                                       0.30
                  Figure 2.   Refinery A—Effluent total  cyanide distribution.2

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00
56
48




40 •

Cfi
O

t '32 •
o
CJ
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o
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\
V
i
i
1
I
|

1
1
1
I
| Total 213 observations
\ from 12/6/73-5/15/75
\ 5 observations > 0.350 mg/2.
\ Mean value = 0.09 tng/i
^x U.S. EPA test tnethod-a^-hr
\ composite samples
	 • 	 ; \
" -V ^
•^

~" — —j-^ __
0.025 0.050 0.10(1 n , 
-------
                        0.500-1
"-O
                   c
                   o
                   •r-l
                   c
                   CD
                   O
                   C
                   Q
G)
•a
•r-l
C
                   U
                        0.400-
                       0.300-
                       0.200-
                       0.100-
                       0.025-
            V-Hai
                                 Dailv Maximum Effluent  Standard - State  of  Illinois-
                                     ill   i  i   i   1  1   I   T
                                    "2    4     6     8     10
10    12


1973
                                                   •1974
                                                              1975
                                                      Time,  months
                                 Figure 4.  Refinery A—Monthly average cyanide discharged.2

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    0.25CH
tc
E
c
o
c
>
CJ
     0.200-
     0.150-
0.100-
     0.050-
     0.025
                    Daily Maximum Effluent Standard - State of Illinois
                                    r
                                    8
                                    T

                                    10
T	T

   12
         1973-*
                        1974
                                                    T—r
    i—i
 4    6


- 1975
-i—n—i—i
 8   10   12
                                       Time, months
                 Figure 5.  Refinery B—Monthly average cyanide discharged.2

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                        TABLE  1.   SUMMARY  OF REFINERY CYANIDE  LEVELS2

Refinery
A
B
C
D
E
F
G
H

Crude Rate
(BPSD)
173,000
154,000
78,000
92,000
102,00
245,000
87,000
37,000

Effluent
Flow Rate
(MGD)
2.6
4.2
6.5
4.7
2.0
6.8
11.4
1.2
Average
Total CN~
Discharged
(mg/Jl)
0.176
0.079
0.008
0.022
0.38
0.045
0.005
0.009
Maximum
Total CN"
Dai ly
Discharge
(mg/fc)
0.650
0.730
0.03
0.210
0.138
0.213
0.035
0.038
Average Total CM"
Discharged
pounds/day
3.8
2.8
0.4
0.9
0.6
2.6
0.5
0.1
pounds/100,000 BPD
Crude
2.2
1.8
0.5
1.0
0.6
1.1
0.6
0.3

Average %
Simple CN" / »
in Discharge •
61
55
—
--
--
71
—

(*)
   As measured by the "Wood River Modification" of the "Roberts-Jackson method".

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STATE AND FEDERAL REGULATIONS REGARDING CYANIDE

     In a review of the existing state water quality and effluent standards,
several of the standards stipulated are below analytical detectability.
Table 2, which summarizes the variety of current regulations, also indicates
that many states rely on a general, all-inclusive sentence to protect waters
from toxic materials.  Water quality standards relating to the bioassay tests
on fish (Louisiana, Texas, New Jersey, Mississippi) are designed specifically
to protect aquatic life.

     In states where a range of values are used for different water quality
designations, drinking water is governed by the higher values of 0.1 to 0.2
mg/£ cyanide.  The lower levels of 0.005 to 0.01 typically represents limita-
tions on streams which are aquatic life habitats.  Only one state, New York,
recognized a different toxicity for iron cyanides.  The number of states
specifying numerical values for their water quality standards is very small
compared to the number of existing regulations.  Even fewer states have adopt-
ed specific effluent limitations for dischargers.  Only Delaware, Illinois,
and Missouri have specified a numerical limitation on cyanide (0.05, 0.025,
and 0.05 mg/i respectively) for dischargers to their state waters.*

     Thus, the states have established low values for water quality and efflu-
ent standards for cyanide in a variety of ways.  While only three states have
specific effluent standards for cyanide, rigorous enforcement of the water
quality standards would require refineries in many states to reduce their
cyanide levels prior to discharging.

     At the federal  level, both effluent and water quality standards will be
adopted as part of the priority pollutant program.  A water quality standard
of 0.005 mg/£ of cyanide was recommended in the "Redbook."3  Effluent stan-
dards,  based on best available technology economically achieveable is requir-
ed by July 1, 1984.*

EXISTING CYANIDE TREATMENT METHODS

     Most exiting cyanide treatment methods are very effective in removing
the simple cyanides.  However, as shown previously in Table 1, refinery
wastes  contain both the simple and the complex cyanides.  While the complex
cyanide accounts for an average of approximately 50% of the total cyanide
discharged by refineries, the percent complex cyanide is highly variable,
ranging from 0% to 100%.  Thus, any proposed treatment scheme must be capable
or removing more than just the simple cyanides from refinery wastewaters.

     Common treatment methods for cyanides include the following:

                      Chemical oxidation
                      Precipitation
                      Ion exchange
                      Biological decomposition
                      Adsorption and catalytic oxidation
*In 1978, the State of Illinois adopted a revised cyanide effluent limitation
of 0.1 mg/£ monthly average and 0.2 mg/l daily maximum.
                                     12

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           TABLE 2.  SUMMARY OF STATE STANDARDS FOR CYANIDE5
Water quality standard                              State
 1.   General standard of free
     from toxic and harmful
     materials
 2.   Not  present  greater than
     1/10 96  hr TLm

 3.   Not  present  greater than
     1/20 96  hr TLm

 4.   Not  present  greater than
     1/10 48  hr TLM

 5.   0.025 mg/H
 6.   None detectable

 7.   0.01 - 0.02 mg/£

 8.   As CN < 0.01 mg/£
     As Fe TCN)6£0.4 mg/A

 9.   0.01 - 0.10 mg/£

10.   0.005 (free) to  0.2 mg/5,
     (total)

11.   0.005 - 0.01 mg/A

12.   0.2 mg/£

 Effluent Standard

 1.   0.05 mg/Jl

 2.   0.025 mg/H

 3.   No other state has specific
     effluent limit on cyanide
Alabama, Arkansas, California,  Colorado,
Connecticut, Delaware, Georgia, Hawaii,
Idaho, Kansas, Maine, Maryland, Massa-
chusetts, Michigan, Missouri,  Montana,
Nebraska, New Mexico, North Carolina,
Rhode Island, South Carolina,  Tennessee,
Utah, Vermont, Washington, Wisconsin,
Wyoming

Louisiana, Texas
New Jersey


Mississippi


Illinois,  Indiana, Iowa, Kentucky,
Pennyslvania, West Virginia

Florida

Minnesota

New York


North  Dakota

Ohio


Oregon

Virginia

State

Delaware,  Missouri

Illinois
                                    13

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Chemical oxidation, most notably alkaline chlorination,  is  very  common  in  the
electroplating industries and is very effective on the simple  cyanides.   For
the complex cyanides, elevated temperatures,  longer holding times,  and  more
oxidant are required.1*6  The presence of organics in refinery wastewaters
limits the practicability of chemical oxidation in the  petroleum industry.
The competitive oxidation rates of the organics result in excessive chemical
consumption in order to destroy the cyanide to acceptable levels.6

     Another alternative treatment method is  precipitation  which is limited
to high cyanide-bearing streams.  This limitation is due to the  solubility of
the metal cyanides formed during the precipitation reactions which is approx-
imately 4 mg/H as CN".7  Ion exchange for the removal of cyanide has limited
application in the refining industry because of the organics present in the
wastewaters.  Fouling and rapid deterioration of the resin  preclude the use
of ion exchange unless extensive pretreatment of the orgamcs  is utilized.

     While the biological wastewater treatment systems present in most refin-
eries remove some cyanide, the mechanisms involved are not  well  understood.
Cyanide removals by biological treatment of greater than 99% have been achiev-
ed in the laboratory utilizing acclimated seed; however, laboratory biological
treatment studies were ineffective in removing iron cyanides.

     One other cyanide treatment method available is adsorption  with or with-
out catalytic oxidation.  Since the method investigated in  this  study can be
classified as an adsorption with catalytic oxidation process,  the existing
technology in this area will be covered in the following section.  Of the
methods discussed, chemical oxidation is the only method currently capable of
achieving cyanide levels of 0.025 to 0.1 mg/£ total cyanide.  Chemical  oxida-
tion of the organics present in the wastewater, however, results in very high
chemical requirements.  Both the production of ozone and chlorine require
energy-intensive processes.  Thus, the existing cyanide treatment methods
available to refineries are both energy and capital intensive.

     If cyanide concentrations lower than those currently achieved are to be
attained, a more economical treatment needs to be developed.  This study
investigates a potential low-cost method based upon adsorption with catalytic
oxidation.  In the following two sections, the previous work in  this area is
summarized, followed by a discussion of the potential low-cost application of
this technology to refinery wastes.

ADSORPTION UITH CATALYTIC OXIDATION

     Activated carbon has been investigated over the past twelve years both
as an adsorbent and as a catalyst for the detoxification of cyanide.
Bucksteeg1* reported that passing potassium cyanide-bearing  wastes over coal
or coke resulted in removals in excess of 98%.  Other forms of cyanide were
also successfully removed by passing the waste over coal and coke.  The mech-
anisms involved, according to Bucksteeg, were adsorption followed by catalytic
oxidation, with the coal or coke acting as the oxidation catalyst.  However,
Bucksteeg concluded that removal of nickel and iron-cyanide complexes were
attributable to only adsorption and not to any oxidation.
                                     14

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     Activated carbon utilized as a catalyst for cyanide oxidation was patent-
ed by Kuhn in 1971.10  The process requires an alkaline pH with a mixture of
air and the cyanide-bearing waste pumped in a pulsating manner through a bed
of activated carbon.  No mention is made in the patent of the type of cyanide
effectively removed, but it is probable that only simple cyanides would be
affected.

     In the early seventies, the Calgon Corporation developed a cyanide detox-
ification method utilizing catalytic oxidation on granular activated carbon.11
Calgon's method is similar to Kuhn's process described previously, except that
cupric ions are added to the wastewater along with oxygen prior to passing
the cyanide-bearing waste through the granular activated carbon columns.
According to Calgon, "cupric ions are added to the water to accelerate and
increase the efficiency of the catalytic oxidation of cyanide by granular
activated carbon."11  In addition to improving the catalytic oxidation of the
cyanide, "the presence of cupric ions results in the formation of copper cya-
nides, which have a greater adsorption capacity than copper or cyanide
alone."11

     Calgon's results indicated that a pH between 6.5 and 8, a constant copper
feed, and dissolved oxygen throughout the carbon bed were necessary for maxi-
mum efficiency.  Adsorption isotherms by Calgon demonstrated that the capacity
of the granular carbon was limited to 2 or 3 mg of cyanide adsorbed per gram
of carbon when no copper was used.  However, the addition of copper increased
the adsorption capacity of 25 mg CN~/gm carbon without any dissolved oxygen
present.  When oxygen was maintained in the system, the adsorption sites were
continuously regenerated through the oxidation of the cyanide.   Tests with a
100 mg/i,  cyanide  in the feed resulted in cyanide effluent levels of less than
0.05 mg/St and low effluent copper levels as long as the adsorption capacity
of the copper on the carbon was not exceeded.11

      In another test by Calgon, ferrous iron and cyanide were  added to water
and pumped  through  a granular activated carbon column, with  high dissolved
oxygen levels.  The  results, shown in Figure 6, indicate that  iron cyanides
can be oxidized, but at a slower rate than the copper cyanides.   Calgon  found
that  iron cyanides  were difficult to treat for the following reasons:

      1.  The  iron  cyanide complex formation competes with  the
         copper cyanide.

      2.  The  iron  cyanide complex  is not adsorbed as well  as
         copper cyanide.

      3.  The  iron  cyanide complex  is more  stable  and  harder  to
         oxidize than  the copper  cyanides.

      4.   Iron will  probably  be  present  at  low levels  in most
         waters.

      Calgon reported the  results  of  a  two-week pilot  study on a coke plant
waste,  which  was  believed  to contain iron  cyanide.   Over the two-week period,
the  cyanide was  treated  from an average inlet of 28.5 mg/A to an average

                                      15

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     5 00-,
                                              Column
                                                Cu

                                                Fe
Initial CN"
 480 rag/I

 500 mg/S,
     400-
     300-
00
E
     200_
     100-
                                           Time  (days)
                      Figure 6.  Iron cyanide disappearance rate.11
Final CN


  3.5

200.0

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effluent of 0.41 mg/fc, or an average 98.5% removal.   Considerable  variation  in
the effluent cyanide concentration was observed, and this was attributed  to
the varying iron concentration of the wastewater^11

     Based upon the work of Calgon, adsorption with catalytical  oxidation is
an effective method of reducing not only the simple cyanides, but  potentially
the complex or iron cyanides which are found in petroleum refinery wastewaters.
Experience in this area is discussed in the following section along with  the
conceptual design for the process investigated in the present study.

POTENTIAL APPLICATION OF ADSORPTION WITH CATALYTIC OXIDATION
TO REFINERY CYANIDE WASTEWATERS

     The results reported by Calgon prompted one refinery to investigate ad-
sorption with catalytic oxidation on their cyanide-bearing waste streams.
The results of this study, as reported by the refinery,  are  presented below.

     "Based upon recommendations made by Calgon Corporation, two carbon  ad-
sorption pilot plants were evaluated.  The first pilot plant was designed to
investigate the merits of carbon adsorption on  a smaller volume, high concen-
tration cyanide bearing  stream.   It was conducted with the cooperation of
Calgon1s environmental engineering department.  The second pilot plant,  con-
ducted without  direct Calgon  supervision, was designed to investigate the
merits  of  carbon adsorption on  an  activated sludge  clarifier effluent.

     Calgon's  analytic  study  of the  first  pilot plant led it to conclude that
catalytic  oxidation of  adsorbed cyanide  in  the  desalter  stripper  bottoms using
granular  activated carbon  could not  be  achieved due to the  presence of the
stable  ferrocyanide complex  (Fe(CN)6-")•   The report indicated  that the  ferro-
cyanide complex can be  removed from the  desalter  stripper bottoms  only  if
either  a  very  equalized  wastewater flow is  obtained over an  extended period
of time,  or a  batch type treatment operation  is installed to maintain proper
pH control,  the iron  cyanide  removal  being  very pH dependent.   The report
also emphasized that  the efficiency of the catalytic oxidation  cannot be
determined unless  the ferrocyanide complex has  been removed.

      The analysis  of  the data from the second pilot plant program indicated
 that carbon,  under limited conditions, will  adsorb cyanide  from the final
 clarifier effluent.  Carbon exhaustion, however, was calculated to be approx-
 imately 11.8 Ib.  carbon per 1000 per gallons of water treated.   Based upon a
 flow rate of 3000 GPM and a total cyanide effluent concentration of 0.175
 mg/A, 51,000 pounds of carbon per day would be required to  remove 6.3 pounds
 of cyanide per day.  Further, attempts to catalytically oxidize the adsorbed
 cyanide, and thus regnerate the carbon, by the presence of  excess oxygen and
 copper failed.  Aeration actually caused an increase in carbon column cyanide
 effluent concentration.  It was determined, therefore,  that a  carbon-adsorp-
 tion system could not produce  sufficient cyanide removal on any practical
 scale under conditions prevalent at the Union  Oil,  Lemont refinery."

      The above results  indicate that equalization  and pH control  are impor-
 tant to the success  of  the system.  This finding  is  similar to Calgon's
 results on the other industrial wastes investigated.11   The increase in
                                      17

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cyanide observed upon aeration suggests that the iron cyanides were being
adsorbed at a faster rate than they were being oxidized.   Thus an accumulation
of cyanides in the column resulted, which, with a change  in conditions, were
released into the effluent.   Assuming that equalization,  pH control, and a bed
of granular activated carbon adequately sized to allow sufficient time for
cyanide oxidation could be provided, the major problem then becomes one of
cost.  The calculated consumption rate of 51,000 pounds per day of granular
carbon places the economics  of this system in the same category, very expen-
sive, as the the high temperature chlorination and ozonation processes.

     Utilizing the findings  obtained to date with adsorption and catalytic
oxidation, a potential low-cost process for removing cyanides from petroleum
refineries was conceived.  The process and its operational advantages are
described in this section prior to presenting the performance results in the
next chapter.

     The major drawbacks with the Calgon process for refinery waste streams
are the large initial capital expenditure and the high rate of carbon exhaus-
tion because of the organic  loading.  The conceptualized  process evaluated in
this study involves the addition of PAC and CuCl2 directly into the activated
sludge unit.  The advantages of this process are a minimal capital expenditure
and potentially a lower carbon exhaustion rate because of biological regener-
ation of the carbon.  There  are numerous other advantages from the addition
of PAC to an activated sludge unit.  If the cost of the carbon is allocated
among all of the benefits, the cost of this cyanide removal process is even
less.  An excellent case history of PAC benefits was provided by Flynn and
Barry.13  A summary of the potential benefits of PAC addition to an activated
sludge plant was provided by Adams;1" and these benefits  are listed below:

     1.  It will improve BOD and COD removals despite hydraulic and
         organic overloads.

     2.  It will aid solids  settling, decrease effluent solids, and
         increased sludge solids.

     3.  It will adsorb dyes and toxic components that are either
         not treated biologically or are poisonous to the
         biological system.

     4.  It will prevent sludge bulking over broader food to
         micro-organism ranges.

     5.  It will effectively increase plant capacity at little or
         no additional capital investment.

     6.  It will provide more uniform plant operation and effluent
         quality, especially during periods of widely varying
         organic or hydraulic loads.

     The potential advantages of the PAC/CuCl2 addition directly into the
activated sludge unit over the previous cyanide destruction methods are num-
erous.  As stated previously, the capital expenditure is  minimal and the

                                     18

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carbon exhaustion rate should be lower, thus consuming less carbon.   In addi-
tion, PAC costs approximately half of the cost of granular activated  carbon
(GAC).  PAC also has a greater surface area per unit weight of carbon.   The
contact times between the cyanide and carbon are longer in the aeration tank
(6-12 hours) as opposed to GAC columns (15-60 minutes), although GAC columns
have a much higher carbon:wastewater ratio.  In addition, the PAC remains in
the system for the time equal to the sludge age (10-25 days) which should pro-
vide a sufficiently long period for oxidation of the adsorbed cyanides.  From
the refinery investigation with the GAC column, the rate of oxidation of the
cyanides may be the limiting loading factor.

     Based upon the conceived process for cyanide removal, this research pro-
gram was undertaken to ascertain the potentail feasibility of the process.
There were numerous questions that had to be addressed before the overall
viability of the process could be determined.  These questions were:

     1.  What effect will the copper have on the overall performance
         of the activated sludge system?

     2.  Can the cyanides be reduced to effluent levels of 0.025 to
         0.1 mg/& total cyanide?

     3.  Will the biological solids (mixed liquor suspended solids,
         MLSS) and/or organics present Interfere with cyanide adsorp-
         tion and/or cyanide oxidation?

     4.  Will the iron cyanides oxidize once adsorbed onto the carbon,
         or will these compounds accumulate in the system?

     5.  Does the removal efficiency increase with higher initial
         cyanide concentrations?

     6.  What fraction of the copper added to the system will be
         present in the treated effluent?

     7.  What influence on the overall performance does each of
         the following variables have:

           •  carbon dose

           •  cupric chloride dose

           •  dissolved oxygen level

           •  mode of cupric chloride addition

           -  PH

           •  hydraulic residence time

           •  sludge age

     If the cyanide can not be treated to levels of 0,025 to 0.1 mg/£, and
the removal efficiency improves with increasing inlet cyanide concentrations,
then in-plant treatment may be more effective.  The cyanide-bearing stripped


                                      19

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sour water is the in-plant stream with the greatest potential.  It could be
treated before or after the desalter, prior to discharging to the sewer.

     A small activated sludge unit could be constructed, designed and operated
to maximize the removal of cyanide through the addition of PAC and CuCl2.
Directing the stripped sour water to the cooling tower and adding low concen-
trations (25-100 mg/&) of carbon and CuCl2 to the cooling water system may
also be feasible and would require minimal capital expenditure.

     Until  the basic chemistry is understood, the most advantageous treatment
scheme can not be determined.  The experimental  program described in the fol-
lowing sections was designed to provide the information needed to evaluate
the process for cyanide removal.  The first phase of the program was directed
toward obtaining a basic understanding of the process.  The second phase then
applied this understanding and the most promising treatment scheme to deter-
mine the overall viability of the process.
                                     20

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                                  SECTION  4

                  EXPERIMENTAL SYSTEMS  AND ANALYTICAL  METHODS
EXPERIMENTAL SET UP

     The experimental  development of design  criteria  for  the  use  of  activated
carbon and cupric ions for the removal  of cyanide  in  an activated sludge waste
treatment system proceeded in two phases.  Phase I  consisted  of 13 batch tests
for the determination  of optimal  levels of the various operational parameters.
This part of the study was conducted in batch reactors with tap water spiked
with known concentrations of cyanide and no  biological activity.   Phase  II
tests were conducted with actual  refinery wastewater  in a continuous flow,
activated sludge waste treatment  system.  The Phase I batch tests were design-
ed to determine the effect of various parameters on cyanide removal.  These
parameters included:

          pH
          Mode of CuCl2 addition
          CuCl2 dosage
          Type of carbon
          Carbon concentration
          Initial cyanide concentration

     The reactors used for the batch tests were fabricated from plexi-glass
and had a total capacity of 12 liters, as shown in Figure 7.   For Phase  I  the
baffle was removed and the overflow ports covered over.   This provided for a
complete mixed batch reactor, the agitation being provided by the air intro-
duced through a diffuser stone located near the bottom of the reactor.  The
agitation took the form of a gentle rolling of the reactor contents.

     The synthetic waste utilized in the batch tests consisted of stock  solu-
tions of potassium ferricyanide and ferrocyanide, (1000 mg/fc as CM"), in
distilled water.  These solutions were prepared on a monthly basis.   The PAC
was treated prior to these tests by first washing in distilled water, drying
in a vacuum oven, then storing in a desiccator until  weighing.

     The cupric  ions were made up in a stock solution of CuCl2 (1000 mg/2. as
Cu) in distilled water, with 2 ml of sulfuric acid (HzSOj added per liter of
stock solution.  Preparation of the reactors for the  batch tests consisted of
the following  procedure.

     1.  Charge  the reactors with 11 a of tap water  (pH^8.3) and
         aerate  for a minimum of one-half hour.


                                     21

-------
ro
ro
                                                                                       Overflow ports
                               Figure 7.   Experimental  reactor.

-------
     2.   Adjust the pH as necessary.   (NaOH  or  H2SOi»)

     3.   Pipette the required  amount  of  cyanide from the  stock solution.
         (1000 mg/£ as CN~)

     4.   Add the CuCl2 from  the stock solution  (1000 mg/£ as Cu) and
         the desired amount  of carbon.

The timing of the tests began  with the addition of the  carbon and CuCl2 to the
system.   The sampling and analytical  methods that  were  used are described
later in this section.

     Phase II consisted of treating actual  refinery wastewater in an  activated
sludge system by the addition  of activated  carbon  and cupric ions.  The single
stage system followed the pattern depicted  in Figure 8  and consisted  of a com-
mon feed tank for two activated sludge units that  operated in parallel.

     The physical equipment utilized  consisted  of  the reactors depicted in
Figure 7.  The reactor compartments were fabricated so  as to maintain 8 liters
in the aeration basin and 2 liters in the clarifier section.  The  baffle was
adjusted for a 1.5 - 2.5 cm space between the baffle  edge and the  reactor
bottom.   The average flow rate through the activated  sludge  system of 19 lit-
ers per day resulted in a detention time of approximately 10 hours  in the
aeration basin and 2.5 hours in the clarifier.

     To initiate biological  activity for the Phase II  tests,  the  aeration
basins were seeded with mixed liquor  suspended solids  obtained  in  part from  a
refinery aerated lagoon and partially from a culture grown,  with  phenols as  a
food supply, in the laboratory.  The feed tank was a  115 liter  polyethylene
tank equipped with a mixer for homogenizing the system.

     The initial feed solution considered was the effluent from the American
Petroleum Institute (API) separator,  which contained cyanide levels of less
than 0.05 mg/£.  In order to accurately assess the potential  of the process,
a higher cyanide level was desired than  was found in the API separator efflu-
ent from this refinery.  Initially, 42 liters of API  separator  effluent,  21
liters of sour water  stripper bottoms, and 21 liters of tap water were charged
to the feed tank three days per week.  The stripper bottoms  had a cyanide
level of 1-2 mg/fc.  The tap water was added to reduce the BOD and phenol  con-
centrations to more "typical" refinery levels.  To prevent the  nutrient level
from being a limiting factor in the activated sludge units,  the phosphorous
level in the feed  tank was increased 2-3 mg/fc  (as P).  The feed composition
was modified approximately half-way through Phase II because the cyanide
removal across the control reactor was over 90% and the effluent cyanides
were thus less than 0.05 mg/X-.  To increase the effluent cyanide levels the
make-up to the feed tank was changed to 21 liters of API separator effluent,
42 liters of sour  water  stripper  bottoms, and  21  liters of tap water.  This
effectively  increased the inlet total cyanide  level to 0.50 - 0.80 mg/fc and
a corresponding  increase  in the effluent cyanide  concentration.
                                      23

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TAP
WATER
i
i

,-\




' " "
t

AP 1
SEPARATOR
i
i








SOUR WATER
STRIPPER
BOTTOMS
i
1
1
i
	 J
FEED TANK
  NO. 1
                       CUPRIC
                       CHLORIDE
                       SOLUTION
             REACTOR
    AIR
                                          — PAC
         REACTOR!/>
                                            y
AIR
                 REACTOR  1
                  EFFLUENT
                  COLLECTOR
            REACTOR 2
             EFFLUENT
             COLLECTOR
Figure 8.   Single stage activated  sludge  system.
                    24

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     The wastewater was pumped from the feed  tank  to  reactors  1 and 2  (control
and test reactors, respectively),  via  a variable speed  "Master-Flex" tubing
pump with dual  pump heads.   The pump was controlled  by  a  30-minute cycle timer
set for pumping 70% of the  time.   The  9 minute  "rest" each  half-hour allowed
the pump and tubing to cool, which provided for longer  tubing  and pump life.

     The sludge age was maintained in  each reactor by wasting  sludge one a
day.  To waste sludge, the  baffle  between the aeration  and  clarifier section
was removed, the side walls scraped to remove any  growth, and  a beaker was
dipped into the reactor to  remove  the  sludge.  The sludge was  poured into a
graduated cylinder for settling,  and the baffle was  then  immediately replaced
between the clarifier and aeration section.  After the  solids  settled  in the
graduated cylinder for one-half hour,  the clear supernatant was returned to
the aeration basin.

     The effluent from each reactor was collected  in 19 liter  glass  bottles
that were held in ice buckets to maintain the integrity of  the samples.  After
the sludge was wasted each  morning, the liquid  level  in the clarifier  was  be-
low the overflow weir for 30 to 45 minutes.  The  composite  samples were  not
started each day until approximately 2 hours after the  sludge  was wasted,  to
allow the system to equilibrate.   Then, each morning, just  prior  to  wasting
sludge the composite samples were taken for the purpose of  daily  analysis.
Thus, the composite samples were only 22 hour samples,  with 2  hours  allowed
each day for the system to settle down after the sludge was wasted.

     Cupric chloride addition to the test reactor was accomplished  by  making
up a 300 m& solution with the desired quantity of copper in it.   This  solution
was then pumped into the test reactor by means  of a small tubing  cassette  pump.
This pump was also controlled by the 30 minute cycle timer.  The  flow  rate was
adjusted so that the CuCl2 solution was completely added during each 24  hour
period.

     The last test in  Phase II consisted of a two-stage activated sludge sys-
tem with cyanide removal in the second  stage.  The feed for the second stage
test was composed of the effluent  from  control  reactor 1 mixed with 5  liters
per day of  stripper bottoms and 15  liters per day of tap water to provide
sufficient  wastewater  for two second-stage units.  Both the second-stage test
and control reactors were operated  with a common  50 liter polyethylene feed
tank.  The  operation of the second-stage test was identical to the previous
tests  described earlier, including  flow rates, copper and carbon addition,
and sample  collection.

SAMPLING PROCEDURE

     This  section  described the procedures followed  for  obtaining samples.
In  Phase I, or the  batch tests, cyanide was  the key  parameter which was moni-
tored.   Grab  samples were  taken at various time intervals  and filtered.  Cya-
nide analyses  were  then  performed  both on  the  filtrate and  on the filter cake.
The filter  cake was resuspended in distilled water  immediately after  filter-
ing and  then  preserved until  the  cyanide  content  was analyzed.  Although three
different  procedures  were  utilized in  attempting  to  determine the quantity of
cyanide  per unit  weight  of solids,  none of the three proved satisfactory.  The


                                        25

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first technique consisted of refiltering the carbon fines from the cyanide
distilling flask after the cyanide analysis was complete.  Control  tests  indi-
cated a loss of solids of 15-25% occurred as a result of this  procedure.
Duplicate solids samples were also collected, one for cyanide  analysis  and  one
for solids analysis.  The variation in the solids level  from duplicate  samples
was approximately 25%.  This third procedure attempted was to  dry and weigh
the solids prior to cyanide analysis, but this also gave very  inconsistent
results.  Additional information on the measurement of the cyanide on the
carbon is included in the following section.

     Phase II tests included the operation of biological waste treatment  sys-
tems.  The actual performances of the activated sludge units were measured  by
the removal efficiences of various pollutants.  All inlet samples were  taken
from the feed tank as grab samples, while the effluent samples were all col-
lected from the 22-hour composite samples.  The inlet and effluents were
monitored for the following pollutants:

                              Sample Frequency,
     Parameter                No. of days/week             Comments

     Cyanide (CN~)                    7                   —	—
     Phenols                          3          Stopped testing for phenols
                                                 midway through Phase II
     Biochemical Oxygen               5                   	
     Demand (BOD5)

     Total Organic Carbon             3          Filtered samples only
     (TOC)                                       during the last test

     Chemical  Oxygen Demand           3          Taken only during last
     (COD)                                       part of Phase II on
                                                 tered samples

     Suspended Solids (SS)            3                   	
As indicated above, only the COD and TOC samples during the last test were
filtered, all other analyses were performed on unfiltered samples.

     The performance of each activated sludge unit was also monitored by
sampling for the following biological parameters.

                              Sampling Frequency,
     Parameter                No. of days/week

     Mixed Liquor Suspended           3
     Solids (MLSS)
     Settleability                    7
     Through-put of treated           7
     wastewater
                                     26

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ANALYTICAL METHODS

     The analytical  methods used during this  program were  in accordance with
those outlined in "Methods for Chemical Analysis  of Water  and Wastes."15 The
cyanide test procedure was modified slightly  to improve  the precision at the
lower cyanide concentrations.   These modifications  to  the  cyanide  procedure
are described below.

Cuprous Chloride Reagent

     The EPA procedure was modified to the following:

     1.  Weigh  10 g of Cu2Cl2 and place in a 500 ml reagent  bottle.

     2.  Wash by decantation four times with  250  ml  portions  of
         dilute sulfuric acid  (HaSOi*, 1+49).
     3.  Wash by decantation two times with 250 ml  portions of
         distilled water.

     4.  Dilute Cu2Cl2 solution to 250 ml  with distilled water.

     5.  Add 50 ml HC1 (sp. gravity 1.19)  and continue adding 1  ml
         portions of the acid until solution is clear and all cyr-
         stals have dissolved.

     6.  Add 100 ml of distilled water and enough HC1 to dissolve any
         crystals that form.  Dilute to 500 ml with distilled water.
         Solution should be light green.

     7.  Store in a tightly stoppered bottle containing a few lengths
         of pure copper wire.

Procedure for Cyanide Analysis

     The EPA procedure is presented below, with the modifications utilized in
this study underlined.

     8.1  Place 500 ml of sample, or an ali quote diluted to 500 ml  in the
1 liter boiling flask.  Add 50 ml of sodium  hydroxide (7.1) tothe adsorbing
tube and dilute if necessary with distilled water to obtain an adequate depth
of liquid in the absorber.  Connect the boiling flask, condenser, absorber
and trap in the train.

     8.2  Start a slow stream of air entering the boiling flask by adjusting
the vacuum source.  Adjust the vacuum so that approximately one bubble of air
per second enters the boiling flask through the air  inlet tube.  Caution:
The bubble rate will not remain constant after the reagents  have been added
and while heat is being applied to the flask.  It will be necessary to read-
just the air rate occasionally to  prevent the solution in the boiling flask
from backing up into the air  inlet tube.
                                     27

-------
     8.3  Slowly add 50 ml 1:1 sulfuric acid (7.5) through the air inlet tube.
 Rinse the tube with distilled water and allow the airflow to mix the flask
 contents for 3 min.  Pour 10 ml of Cu2Cl2 reagent (7.4) into the air inlet
 and wash down with a stream of water.

     8.4  Heat the solution to boiling, taking care to prevent the solution
 from backing up into and overflowing from the air inlet tube.  Reflux for two
 hours.  Turn off heat and continue the air flow for at least 15 minutes.
 After cooling the boiling flask, disconnect absorber and close off the vacuum
 source.

     8.5  Drain the solution from the absorber into a 250 ml volumetric flask
 and bring up to volume with distilled water washings from the absorber tube.

     8.6  Withdraw ^0 ml of the solution from the volumetric flask and trans-
 fer to a _50 ml  volumetric flask.  Add 15 ml of sodium phosphate solution (7.6)
 and 2.0 ml  of Chloramine T solution (7.12) and mix.  Immediately add 5.0 ml
 pyridine-barbituric acid solution (7.13.1), mix and bring to mark with dis-
 tilled water and mix again.

     8.7  When  using pyridine-barbituric acid, allow 6^ minutes for color de-
 velopment than  read absorbance at 578 nm in a 1.0 cm cell  immediately.

     8.8 -  8.11  No change in USEPA procedure.

     The above  modifications to the EPA procedure have been utilized by some
 of the refineries in Illinois, and the refineries have found that the above
modifications yield more precise results on refinery wastewaters.  Two types
 of distilling glassware set-ups are recommended by the EPA test method.  The
 one utilized in this project is shown in Figure 9.

     Other  analytical  equipment utilized was a Beckman Model 915 for total
organic carbon  (TOC) measurements.  Copper analyses were performed on a
 Perkin Elmer Model  360, Atomic Adsorption Spectrophotometer.
                                    23

-------
COOLING WATER
INLET TUBEv
      HEATER-
                                        TO  LOW  VACUUM
                                           SOURCE
                                   - ABSORBER
                              DISTILLING FLASK
                    O
     Figure 9.  Cyanide distillation apparatus
                                            15
                            29

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                                  SECTION 5

                        PHASE I  -  EXPERIMENTAL  RESULTS

     The initial  phase of the experimental  work was  limited  to  defining  the
basic chemistry of the process.  A series of batch tests  using  tap water
spiked with potassium ferrocyanide or potassium ferricyanide were performed
to delineate the importance of the following five operating  variables:

     1.  pH

     2.  copper dosage

     3.  mode of copper addition

     4.  carbon dosage

     5.  type of carbon

     For each test, filtrate samples for cyanide analysis were  collected at
times of 15 minutes, 1 hour, 6 hours, 24 hours, and  for certain tests  48 hours
and/or 72 hours.   Carbon samples were also periodically collected for  deter-
mination of the quantity of cyanide remaining on the carbon. Of the 275 cya-
nide analyses performed during Phase I, (excluding standards),  79% of  the
samples were analyzed within 24 hours, 12% were analyzed  within 48 hours, and
the remaining 9% were analyzed within 72 hours.  All  of the  filtrate samples
were analyzed withing 48 hours,  thus only certain carbon  samples were  held 72
hours before analysis.

DESCRIPTION AND RESULTS OF BATCH TESTS

pH Effect on Cyanide Removal

     The first operating variable  investigated  in the batch  tests was  pH.
Two types of cyanide, potassium ferrocyanide and potassium ferricyanide, were
utilized each at three pH levels.   The results  of the ferrocyanide test  runs,
presented graphically in Figure 10, indicate that the rate of cyanide  removal
increased with decreasing pH.  The equilibrium  cyanide level, which was  con-
sidered to occur after 24 hours, was lower at the lower pH level.  The lower
pH apparently changed the surface  properties of the  PAC resulting in a greater
affinity for the iron cyanides.

     The results of the ferricyanide tests were found to  be  very similar to
the ferrocyanide tests, as expected.  This second series  was only carried out
for 6 hours, with the results shown in Table 3.


                                      30

-------
0.4
0.3- •
0.2 -
0.1 - •
                 0.25
D - pH JO.2,  initial CN~* 0.53  mg/f

A - pH  7.9,  initial CN~  0.48  mg/i

O - pH  2.5,  initial ClT  0.4S  mg/i.


Each reactor contained 250 mg/f.  lignite
based carbon and 1.5 mg/£ cupric  ions.
                                                         rerrocyanide, measured as  CN
                                               Time, hours  (Log scale)
                            Figure 10.   pH  effect on  rate  of cyanide removal.

-------
           TABLE 3.  RESULTS OF pH  EFFECT ON  FERRICYANIDE REMOVAL
Time ,
hr
0
1
6
Reactor No. 1
pH 3.0
0.44
<0.01
<0.01
Reactor No. 2
pH 7.0
0.50
0.11
0.01
Reactor No. 3
pH 11.0
0.52
0.09
0.05
Thus, ferricyarn'de removal  is  influenced  by pH  in  a  similar manner  to  ferro-
cyanide; that is, the lower the pH,  the more rapid is  the  adsorption rate,
and the higher the removal  efficiency.

     While the low pH is desirable for maximum  cyanide removal,  effluent cop-
per levels at the low pH are unacceptable.   Filtrate samples  collected at 6
hours for copper analysis are  presented  in  Table 4.   The low  pH  range  of 2.5
to 3.0 resulted in effluent copper concentrations  of 1.7 to 1.8  mg/&,  as com-
pared to 0.02 to 0.05 mg/£ at  the higher  pH values.

                   TABLE 4.  FILTRATE COPPER LEVELS, mg/fc

ferrocyanide
f err i cyanide
Reactor No. 1
pH 2.5 - 3.0
1.8
1.7
Reactor No. 2
pH 7.0 - 7.9
0.05
0.05
Reactor No. 3
pH 10.2 - 11.0
0.05
0.02
     While cyanide removal  is enhanced in lower pH solutions,  copper  levels
are not satisfactory.  At near-neutral pH values,  cyanide removals  of 94%  and
98% were obtained, while filtrate copper levels of 0.05 mg/A were also
achieved.  Thus, for the remainder of Phase I,  no  pH adjustments were made,
and the ambient pH ranging from 8.3 to 8.4 was  considered adequate  for the
remaining batch tests.

Effect of Cupric Ion Concentration and Mode of  Addition
on Cyanide Removal

     Copper can be added by two different techniques, either directly into
the aeration basin or adsorbed onto the carbon  prior to addition of the car-
bon.  Two series of tests were conducted to determine if there was  any sign-
ificant difference in the mode of addition.  Each  reactor was  charged with
250 mg/& of lignite-type carbon, and ferrocyanide  was used as  the cyanide
source.  The results are presented in Tables 5  and 6.
                                    32

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TABLE 5.  MODE OF COPPER ADDITION,
   1.5 mg/fc INITIAL COPPER DOSE
Time,
hr
0
0.25
1.0
6.0
48
72
Cyanide concentration, mg/£
Copper
adsorbed onto
carbon
0.52
0.065
0.16
<0.01
—
<0.01
Copper added
directly
to basin
0.39
0.060
0.040
<0.01
—
0.013
Copper added
directly
to basin
0.39
0.040
0.025
0.010
<0.01
—
TABLE 6.  MODE OF COPPER ADDITION,
   0.5 mg/fc INITIAL COPPER DOSE
Time,
hr
0
0.25
1.0
6.0
48
Cyanide concentration, mg/£
Copper
adsorbed onto
carbon
0.48
0.20
0.16
0.17
0.12
Copper added
directly
to basin
0.37
0.17
0.13
0.14
0.12
                33

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     Tables 5 and 6 indicate there is little, if any,  difference  in  cyanide
removal based upon the mode of addition.   The filtrate copper  levels were also
unaffected by the mode of addition.  No further studies were conducted  regard-
ing the mode of copper addition, and for  the remainder of the  tests, the cop-
per was added directly into the aeration  basin.

     Numerous tests were conducted with varying copper concentrations,  and the
typical results are presented graphically in Figure 11.  (All  of  the test
results are tabulated in the Appendix).  Such data indicated that the copper
dose is an important factor in the cyanide removal process.  For  example, with
250 mg/Jl of the lignite carbon and no copper, an equilibrium cyanide level of
0.28 mg/£ was achieved; however, with 1.0 mg/£ copper  added  to the same system,
equilibrium cyanide levels as low as 0.010 mg/5, were achieved.

Effect of Carbon Type and Concentration on Cyanide Removal

     Two types of PAC, lignin-based (Aqua Nuchar) and  lignite-based  (Hydro-
darco C), were evaluated.  The performance of each carbon type was evaluated
throughout a range of carbon and copper concentrations.  The cyanide levels
achieved with each carbon are presented in Figure 12.   The lignite-based car-
bon appeared to be superior for all levels tested; however,  as the copper
dosage increases, the effect of carbon type is diminshed.

     Utilizing 1.0 mg/S, of the copper and ferricyanide as the  new cyanide
source, the lignin and lignite carbons were compared at a dosage  level  of
250 mg/£ carbon.  The results from this test are presented in  Table  7.  The
lignin carbon was found to be more effective on the ferricyanide, which is
just the opposite of the results with the ferrocyanide.

     Comparing Figure 11 to Figure 12, it is apparent  that the equilibrium
cyanide achieved is influenced more by the copper dosage than  by  the carbon
dosage.  For example, a doubling in the copper level has a much greater impact
on the final cyanide concentration than does a doubling in the carbon level.
These qualitative deductions are further  confirmed in  the regression analysis
which follows.

     The carbon dosage also influences the copper concentration found in the
filtrate, as does the initial copper dosage.  While the above  results suggest
that higher inital copper and lower carbon levels may  be the most economical
approach for removal of cyanide, the filtrate copper level is  a limiting con-
straint, which is discussed in the following section.

Effluent Copper Levels

     The "acceptable" effluent copper level  is an important  operating con-
straint and will vary for each refinery,  depending upon water  usage, state
effluent standard, and water quality standards.  An effluent copper  level of
0.1 rng/A was assumed to be an "acceptable" level  to achieve  during the  batch
tests, and this was used as a criterion for all filtrate samples.

     The filtrate copper samples, all collected 6 hours after  initiating each
batch test, are summarized in Table 8.  Data for both  carbon types were

                                     34

-------
LO
in
                  o

                  en
                  00
                  c
                  o
                  •H
                  C
                  O
                  u
                  c
                  o
                  u

                  01
                  -o
                  •f-l
                  c
                  C3
                  >,
                  u
0.1--
                                                   D-  100 mg/£  lignite  carbon



                                                   A-  250 mg/2.  lignite  carbon
                                                   Initial  ferrocyanide  concen-

                                                   tration, 0.5 + 0.05 mg/l as

                                                   CN~.
                                       0.5             1.0            1.5


                                    Initial Copper Concentration,  mg/£
                          Figure  11.   Equilibrium cyanide levels as a function of copper dose.

-------
                       0.5
                       0.4 • •
Co
                   z

                   m
0.3- -
                      0.2 - -
                      0.1 .
                                                                               O- 0.5 mg/J. Cu  , lignin  carbon
                                                                                             ± i
                                                                                 - 0.5 mg/8. Cu  , lignite carbon
                                                                                             ii
                                                                               • - 1.0 mg/J Cu  , lignin  carbon
                                                                                             1 I
                                                                               A- 1-0 mg/J. Cu  , lignite carbon
                                                                               Initial ferrocyanide concentration
                                                                               0.5 + 0.05 rag/£ as CN~
                                  100
                                          200
                                                   300       400      500     600

                                                         Carbon Concentration, mg/J.
                                                              700
                                                                               900
                                                                                       1,000
                    Figure  12.  Equilibrium  cyanide levels as  a  function of carbon concentration.

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 TABLE 7.  EFFECT OF CARBON TYPE
     ON FERRICYANIDE REMOVAL*
Time,
hr
0
0.25
1.0
6.0
24
52
72
Cyanide concentration, mg/A
Lignite carbon
0.63
0.22
0.28
0.29
0.29
0.28
0.34
Lignin Carbon
0.67
0.21
0.20
0.15
0.11
0.10
0.10
*Copper dosage 1 mg/1
 Carbon dosage 250 mg/1
                37

-------
combined since there was not sufficient information  to  distinguish any  differ-
ence in copper level for the two carbons.   As  the  carbon  dosage  increases, as
shown in Table 8, the effluent copper level  decreases.

                       TABLE 8.   FILTRATE  COPPER LEVELS
Initial
copper
cone., rng/A
0.5
1.0
1.5
Average filtrate copper levels, mg/A
100 mg/&
carbon
0.06
0.08
0.19
250 mg/fc
carbon
0.05
0.05
0.09
1,000 mg/£
carbon
< 0.02
0.02
—
To maintain a low copper outlet concentration at the 1.0 and 1.5 mg/£ inlet
copper dosage, higher carbon levels are required.  At the 1.5 mg/£ copper dos-
age, the 0.1 mg/fi, filtrate constraint was exceeded when 100 mg/£ carbon was
utilized.  At the 250 mg/J, carbon and 1.5 mg/fc initial  copper dosage, the
average filtrate copper level was below 0.1 mg/& .  Thus, to insure compliance
with the 0.1 mg/£ copper constraint, 1.0 mg/£ initial copper was selected as
the maximum dosage level.  As will  be discussed in the following section.
1.0 mg/& copper dosage is also a limitation of this process if it is to be
utilized in the biological waste treating units.

COPPER TOXICITY

    The concept of introducing a toxic substance (copper) into an activated
sludge system to enhance removals of cyanide, at first glance, does not appear
to be an acceptable technique for cyanide removal.  Supervisors of refinery
wastewater treatment plants will need assurance that such a practice will not
affect the biological activity in the activated sludge units.  Phase II of
this study supplies part of this assurance, but a brief review of copper
toxicity is provided to delineate the importance and aspects of the toxicity
effects of copper.

    In the carbon-copper process, a uniform copper dose will be added to the
aeration basins; thus, the micro-organisms will become acclimated to the cop-
per level maintained in the system.  Acclimation of the micro-organisms to
copper was shown to be important in several studies.  For example, Sudo and
Aiba*6 reported that a copper dosage of 0.25 mg/fc copper added to an unaccll-
mated culture of protozoa reduced the growth rate by 50%.  However, acclima-
tion for 96 hours to the copper increased the concentration at which 50%
reduction in growth rate occurred by a factor of 1.2 to 2.2

    Poon and Bhayani17  also investigated the effect of slug doses of copper
on both sewage bacteria and the fungus Geotrichum candidum.  The authors hypo-
thesized that metal toxicity could selectively kill off certain sewage
                                     38

-------
organisms, permitting an overgrowth of poor settling fungus.   Poon  and Bhayani
found that slug doses of copper up to 25 mg/Jl did  not show  any  obvious toxic
effects on the sewage bacteria; however, toxic effects on the  fungus were ob-
served from the slug doses.   Experiments performed by Lamb  and Tollefson18
indicated that there can be  short term impacts.  Slug doses of 5 mg/H of cop-
per decreased glucose conversion rates by 75% in the first  hour  after which
recovery occurred.  Thus, acclimation of the micro-organisms to  copper appears
to reduce the manifestations of toxic effects.

     The concern in utilizing the PAC-copper system will  be the  long-term
effects of copper addition into an activated sludge unit.   Salotto, et al.,19
found no apparent effect from adding 1 mg/& copper to an  activated  sludge unit
over a 2-1/2 month period.  At a continuous feed of 5 mg/Z  over  a  six-month
period, the authors found the COD removal dropped  from 88%  (control test) at
the high organic loadings to 84%.  At low organic  loadings, the  COD removal
efficiency dropped from 86%  (control) to 67% with  a 5 mg/il  copper  feed.   It
was noted in this study that the setteability of the sludge was  slightly  im-
proved with the copper addition.

     A follow-up study by Barth, et al.,20 concluded that,  "one  mg/Z in  the
influent sewage is the threshold dose for the aeration phase."  An increase
in effluent COD values were  observed (over the control) when the influent cop-
per level was maintained at  1.2 mg/A for 60 days.   This study also reported
the biological floe settled  rapidly and no bulking was encountered in the
metal-fed system, whereas the control units frequently bulked.  Anaerobic
digesters fed the metal-containing sludge showed no inhibitory effect,  even
with total copper levels as  high as 196 mg/& were measured  in the sludge.

     Thus, based on the literature, influent copper levels  below 1 mg/& should
have no noticeable effect on the performance of activated sludge units.   In
addition, the activated carbon added to the basins should adsorb the bulk of
the copper, thus reducing the quantity available to be adsorbed by the bio-
mass.  As a limiting constraint  for Phase II, 1 mg/& copper was  considered  the
maximum permissible influent dose.  Based upon the batch tests reported in
Phase  I, however, 1 mg/fc copper  should be sufficient for high levels of cya-
nide removal.

REGRESSION ANALYSIS
           *

     The experimental results obtained  in Tests 1 through  12  provided useful
data regarding the  importance of five operating variables,  pH, carbon concen-
tration and type, copper concentration, and mode of copper addition.  To
quantify the  effect of  each variable on cyanide removal efficiencies, statis-
tical  analyses were performed.   Two variables,  pH and  the  mode  of  copper addi-
tion,  were ultimately excluded  from the  statistical  analysis.   The mode of
copper addition did not  influence  cyanide removal and  only a  limited number of
tests  were performed at  low and  htgh  pH  ranges.   The  data  set,  which consisted
of 23  observations, also limited the  number  of  variables to be  tested.
                                     39

-------
     Two cyanide removal mechanisms were hypothesized using the factors  con-
trolled during experimentation.  These mechanisms are shown below:

          [CN-]e  =  [C]a  [Cu]6  [CNTj  [CT]                       (1)

          [CN-]e  =  a  + a[C]  +  3[Cu]  +  yCCN ]1  +  [CT]       (2)

where:

     a,3,Y»o = constants

     [C]     = carbon concentration

     [CM]..   = initial  cyanide concentration

     [CN]g   = equilibrium cyanide concentration

     [Cu]    = copper concentration

     CT      = carbon type

Eq 1 indicates that the outlet cyanide concentration is a multiplicative
function of the concentrations of inlet cyanide, copper, and carbon,  plus
carbon type.  In Eq 2,  a linear relationship-is assumed for the range of con-
centrations considered.  Each of these hypotheses was tested using  multiple
regression techniques.   Through statistical  analysis, the effect and  signifi-
cance of these operating variables was identified.  These results are depicted
in Table 9.

     The statistical  criteria for evaluation were the t-statistic,  size  of
residuals, and adjusted R2 value.  In Table 9,  the t-statistic, which is a
measure of the significance of the variable, is found below the coefficient
in parentheses.  For the 23 observations less  the number of variables and  at
the 5% significance level, an approximate t-value of 2.1 indicated  a  signifi-
cant variable.   As different combinations of variables were considered,  the
size of the residuals and R2 factor were monitored.   The equation in  which
the best fit (lowest residuals and highest adjusted  R2) of the  data was  then
identified.

     The first six rows of Table 9 illustrate linear relationship regressions
of the operating parameters.  Line 4 presents  the most satisfactory explana-
tion of the effects of operating variables.   Line 6  is the same equation as
Line 5 in Table 9, but  with the dependent variable,  the cyanide concentration,
at 6 hr.  Uith the exception of Line 6, all  of the other regressions  utilized
the equilibrium cyanide values (average of the  24, 48, and 72 hr. cyanide
samples) as the dependent variable.   Line 4 presents the most satisfactory
explanation of the effects of operating variables.  Initial  cyanide,  carbon,
and copper concentrations are very important in determining the equilibrium
cyanide concentration.   The ensuing equation is a translation of the  regres-
sion results of Line 4  into operating limitations.
                                     40

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                                          TABLE 9.  SUMMARY OF REGRESSION ANALYSES
Dependent Variable
1. Equilibrium [CN~]
2. Equilibrium [CN~]
3. Equilibrium [CN~]
It. Equilibrium [CN~]
5. Equilibrium [CN~]
6. Six-hour CN~
Equilibrium
7. Log
[Equilibrium CN ]
8. Log
[Equilibrium CN ]
Carbon
Cone.
-0.000236
(-2.077)*
-0.000337
(-3.72)
-0.000268
(-3.16)
-0.000305
(-4.83)
-0.000272
(-4.70)
-0.000342
(-4.77)


Copper
Cone.



-0.150
(-4.93)
-0.155
(-5.09)
-0.180
(-4.78)


Ratio
Carbon/Copper
1.77 x 10~5
(1.333)
1.81 x 10"5
(1.89)
1.51 x 10~5
(1.53)





Type of
Carbon
(dummy)**

-0.0868
(-1.697)

-0.0442
(-1.22)


-0.11J 6
(-2.35)

Initial
[CN-]

0.6065
(3.080)
0.761
(4.16)
0.703
(5.04)
0.779
(6.19)
0.843
(5.39)


Log[ Carbon]






-1.558
(-5.018)
-1.353
(-4.078)
LogfCopper]






-0.403
(-3.12)
-0.363
(-2.55)
Log [Initial CN~]






2.604
(2.614)
3.632
(3.646)
Constant
0.178
(4.418)
-0.0391
(-0.306)
-0.194
(-2.05)
0.0309
(0.333)
-0.0423
(-0.589)
0.0074
(0.081)
7.9
(4.24)
6.79
(3.37)
Adjusted
R2
0.13
0.56
0.52
0.78
0.77
0.74
0.66
0.58
 * t-statistic shown in ( ).
** Note:   The dummy value used for lignite-based carbon was  1 and  lignin-based carbon was  0.   Coefficients  appearing  in  this  column  are
          based on these values.

-------
          [CN]   =  0.703[CN ]. - 0.000305[C]  - 0.150[Cu]  - 0.0442[CT]  (3)
where:

     [CN]   =  equilibrium cyanide concentration, mg/S,
     [CN]i  =  initial  cyanide concentration,

     [C]    =  carbon concentration, mg/Ji

     [Cu]   =  copper concentration, mg/£

     [CT]   =  carbon type, lignite = 1 and lignin = 0

Eq 3 is expressed as a  linear relationship of the operating variables.   This
equation is appropriate only within the range of initial  cyanide concentrations
tested (0.3 to 0.7 mg/£) and copper concentrations below  1.5 mg/JL   Figures
11 and 12, presented earlier, did show a linear relationship between these
separate variables and  cyanide over certain concentration ranges.

     This analysis has  shown that the effect of copper concentration,  carbon
concentration, and initial  cyanide concentration are all  statistically  signi-
ficant in influencing the equilibrium cyanide concentration.  Carbon type was
not demonstrated to be  a statistically significant variable at the  5%  signifi-
cance level in the "best fit" equation (No. 4 in Table 9), although the t-sta-
tistic for carbon type  was  significant at the 5% level  in Eq 7 and  at  the 20%
level in Eq 2.  The limited number of tests with the lignin carbon  could
account for the lower t-statistic associated with carbon  type.

     Rows 7 and 8 of Table  9 represent regressions performed assuming  a rela-
tionship similar to Eq  2.  The results of these analyses  also support  the
conclusion that carbon  and  copper enhance the removal  of  cyanide.   The  multi-
plicative function utilized did not explain as much of the variance in  data
points as the linear function.  This conclusion, however, may be due to the
limited range in which  each variable was tested.

     Implications of these  analyses are that by increasing copper and  carbon
concentrations the cyanide  effluent level can be reduced.  As pointed  out
previously, and verified by the size of the coefficients  in Table 9, the
copper has a stronger influence on the equilibrium cyanide than does carbon
concentration.  From a  economic point of view, this is a  favorable  result.
Doubling the copper dosage  (say, from 0.5 to 1.0 mg/5,)  is more economical
than doubling the carbon dosage (say, from 250 mg/H to 500 mg/A) , and  the
resulting reduction in  the  equilibrium cyanide level  is much greater with
the copper.

     The overall statistical analysis supports the conclusions reached  in the
previous section.  With only 23 sets of data and testing  up to five variables,
the regression results  are  significant.  To explain 78% of the variation in
the results with the tested four variables is a strong implication  that the
important operating factors have been determined.   Especially considering the
scatter in the analytical procedure for cyanide, the quantitative results

                                     42

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verify the importance of the initial  cyanide,  copper  and  carbon concentrations
as driving forces.

     Included in the Appendix is  a summary table of all batch  tests  performed.

OXIDATION OF CYANIDE ADSORBED ON  THE  CARBON

     In the previous sections, the PAC-copper  system  was  shown to  be highly
effective in removing cyanide, with removals  below 0.01 mg/£ achieved from
starting solutions  containing approximately 0.5 mg/fc  iron cyanides (as CN").
The economics of PAC/CuCl2 addition are primarily a function of the  carbon
requirements.  If cyanide is oxidized once adsorbed,  then the  carbon require-
ments are reduced and, thus, the  economics enhanced.   In  the background sec-
tion the oxidation  of iron cyanides was reported to occur, but at  a  slower
rate than ulher complex cyanides.

     During approximately half of the batch tests an  attempt was made to de-
termine the rate of cyanide oxidation.  To analyze for the amount  of cyanide
remaining on the carbon, the following procedure was  used.  The  carbon samples
were obtained by filtration, then resuspended in distilled water  and placed  in
the cyanide distilling flask.  After  the analysis, the carbon  was  refiltered,
dried, and weighed.  The cyanide  adsorbed per gram of carbon was  recorded.
The cyanide remaining in solution (not adsorbed) was  also noted.   Except for
the beginning of each test the amount of cyanide remaining in  solution was
typically less than 10% of the total  cyanide.

     The results from these experiments are summarized in Table  10.   (The data
utilized to develop Table 10 are  tabulated in the Appendix.)  Assuming a first
order decay reaction, the following equation was utilized to determine the
cyanide decay rate:
                                       Coe'kt                         (4)
where:
     CQ is the initial CN' loading

     Ct is the CN~ loading at time t

     t is the time in days

     k is the decay rate

The decay rate, k, was computed using the least-squares fit procedure for each
test.  The coefficient of determination, or R2, was also computed for each
test, and these results are  included 1n Table 10.  The R2 coefficients varied
considerably from test to test, and the results from  four of the tests showed
an R2 value of less than 0.4, indicating considerable scatter 1n the data.
This scatter is attributed primarily to inconsistencies in individual test
results and the small sample size.  The decay rates ranged from -0.01 to
-0.16 day1, with a mean of  -0.06 day-1.  If the  four tests with R2 values
less than 0.40 are omitted,  the recomputed mean decay rate 1s -0.08 day-1.

                                     43

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               TABLE 10.  BATCH TESTS - CYANIDE DECAY RESULTS
Test
reactor no.
1-1
1-2
1-3
3-1
3-2
4-1
4-2
4-3
7-2
7-4
9-2
9-3
9-4
No. of data
points
4
4
4
5
6
7
5
6
3
3
5
6
5
Decay rate^1'
(day1)
-0.06
-0.16
-0.12
-0.08
-0.03
-0.01
-0.01
-0.05
-0.07
-0.08
-0.06
-0.07
-0.02
Intercept'2'
(C/Co)
0.90
0.96
0.96
0.83
0.97
0.95
0.96
0.96
0.97
1.00
0.90
0.96
0.80
Coefficient of
determinations, R2
0.22*
0.98
0.97
0.44
0.85
0.22*
0.16*
0.87
0.85
0.99
0.70
0.89
0.31*
                                          kt
(i)   Based on first order decay;  C^ = Coe


(2)   Intercept is from plotting In (C/C ) vs time.

     intercept,  C/CQ = CQ/CO = 1        °


 *   Coefficient of Determination (R2)  below 0.40
For a perfect fit the
                                      44

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     The results of Bernardin,11  shown in Figure 6,  indicated  an  iron-cyanide
decay rate of -0.13 day-1.   However,  Bernardin was working with an  initial  con-
centration of 500 mg/H iron cyanides  as compared to  the 0.5 mg/£  iron  cyanide
utilized in this study.  The thousand-fold difference in starting concentra-
tions could account for the difference in the observed decay rates.   Perhaps
the most significant finding from these tests is that iron cyanides  are  des-
troyed by the PAC/CuCl2 process;  however, the rate of decay appears  to be
slower than that reported by Bernardin.11

CONCLUSIONS FROM PHASE I

     The primary goal  of Phase I  was  to develop a basic understanding  of the
chemistry involved in  the PAC-copper  system for cyanide removal.  The  results
of the regression analysis  indicate that this primary goal was achieved.

     From the information collected in Phase I, the  optimal location in  a
refinery for treatment of the cyanides was also ascertained.  Based upon the
findings in Phase I, the logical  first choice for cyanide removal would  be the
biological wastewater  treatment (or activated sludge) unit.  Cyanide treatment
at this location would require little or no capital  expenditure,  (unless PAC
thermal regeneration is desired).  The following four possible constraints
were identified that could  preclude cyanide treatment in the biological  units:

     1.  Cyanide level below 0.1  mg/A may not be achievable because
         of a concentration-limiting  phenomenon.

     2.  A pH outside  the range 6.5 - 8.5 is required for removals
         of cyanide below 0.1 mg/H.

     3.  Copper levels in the treated effluent are  greater than
         0.1 mg/A.

     4.  The copper levels  required for cyanide removal may be high
         enough to be  toxic to the micro-organisms.

     These constraints were addressed in the Phase  I research.  Cyanide  remov-
al does not appear to  be a  concentration-limiting phenomenon.   In the batch
tests cyanide removals well below 0.1 mg/l were also achieved  at  the higher
carbon and copper dosages investigated.  The experimental results also indi-
cated that pH values between 6.5 and 8.5 are satisfactory for  cyanide removal.
Copper dosages up to 1.0 rng/H were effective in reducing the cyanide to  below
0.01 mg/H and yet maintaining effluent (or filtrate) copper levels  less  than
0.1 mg/Z.  Copper levels above 1.0 mg/i improved cyanide removal, but efflu-
ent copper levels also increased.

     The fourth constraint  regarding the potential  toxic effects  of copper on
the activated sludge bio-mass was partially answered by the literature review.
Confirmation of the copper  toxicity literature, however, required the results
of Phase II.
                                     45

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                                  SECTION 6

                        PHASE II - EXPERIMENTAL RESULTS


     From the results of the first phase, the PAC/CuCl2 process looked very
promising as a cyanide removal technique.  There were still many unanswered
questions about the process which could not be resolved by batch tests with
synthetic wastewaters.  Phase II, which utilized a continuous flow biological
treatment systems with actual refinery wastewater, was designed to provide
information pertaining to the final feasibility of the system.

     The Phase I conclusion that the activated sludge system would be the most
economical  treatment location for the cyanide removal system raised several
questions.   First, what effect would copper have on the micro-organism?  The
literature indicated that levels below 1.0 mg/Ji have little or no effect; how-
ever, none of the copper toxicity studies utilized refinery wastes.  Another
area of concern was the impact of the organics present in the refinery waste-
water on the adsorption of the cyanides by carbon.  Competitive adsorption
between the organics and cyanide for the active carbon sites could potentially
reduce the effectiveness of this process to an uneconomical level.  Determin-
ing the answers to such questions and describing the overall feasibility of
the process were established as the goals of Phase II.

     In the second phase of laboratory operation, two laboratory scale acti-
vated sludge units were set up in parallel.  Reactor #1 was utilized as a
control reactor; that is, no PAC or CuCl2 was added to this reactor at any
time.  With the exception of the PAC and CuCl2 addition, the control and test
reactors were operated in a like manner.  In addition to monitoring the cya-
nide levels, the following parameters were measured to assess the impact of
the PAC/CuCl2 addition on the biological system.

                       Biochemical Oxygen Demand (BOD5)
                       Total Organic Carbon (TOC)
                       Phenols*
                       Suspended Solids (SS)
                       Chemical Oxygen Demand (COD)**

     A total of five tests were carried out in Phase II.  The first test was
designed as a control test, the next three tests evaluated various carbon and
copper loadings, and the last test evaluated a two-stage activated sludge pro-
cess.  By investigating the different combinations of PAC and CuCl2 as
* First part of Phase II only
**Last part of Phase II only
                                     46

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indicated in the test conditions  summary,  sufficient  data  could  be  generated
for determining  the  feasibility of  the  process.

                             TEST CONDITIONS  SUMMARY



Test

A
B
C
D
E

Number of
Days of Test

21
19
14
41
12
Level of
CuCl2
Addition*
(nig)
0
0.5
0.5
1.0
1.0
Level of
PAC
Addition*
(ing)
0
14
39
39
25

Sludge
Age

12
12
15
15/10
15

             *per liter of feed

     Each of the tests is described in detail in the following sections.
These experimental results provide a general overview on the applicability
and feasibility of the PAC/CuCl2 system.

TEST A - CONTROL TEST

     Refinery wastewater feed to Reactors #1 and #2 was initiated on February
8, 1976.  Both reactors were initially seeded with approximately 1000 mg/Jl
mixed liquor suspended solids (MLSS).  The sludge wastage rate was gradually
increased over a 10-day period until the equilibrium wastage rate was attain-
ed.  The sludge age was then maintined at 12 days in both reactors during
Test A.

     Test A, initiated on February 19, lasted for a 21-day interval, and
Table 11 summarizes the operating performance obtained during this period.
As would be  expected, the removal performance of both reactors was very simi-
lar for all  parameters.  Phenol removal was  very high with an average inlet
level of 41  mg/Jl and all effluent samples below 0.1 mg/£.  Cyanide removal
was higher than anticipated with an average  removal of greater than 90% across
both reactors.  The only parameter which varied from design was  feed through-
out.  The flow rates of 16.0 and 16.7  I/day  for Reactors 1 and 2, respectively,
were both below the design 19 I/day because  of pump failures on  three occa-
sions.  As operating experience was gained,  the frequency of the pump failures
was minimized reducing the fluctuation  in flow rates.

     Statistical analysis of the experimental data  from Test A provided con-
firmation of the operational similarity of  the control and test  unit prior  to
initiation of the  test program.  The mean cyanide values of the  two units were
compared using two statistical tools,  a t-test and  the paired observations.
These tests  also  provided a measure of the  comparability of the  biological
                                      47

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                     TABLE 11.  TEST A - SUMMARY RESULTS
Parameter
Flow rate, I/day
Cyanide, mg/H
BOD, mg/SL
TOC, mg/£
Phenol , mg/H
Copper, mg/Z
Suspended Solids,
mg/l
Mean Values
Inlet
_
0.17 ± 0.04
250 ± 120
111
41
<0.02
25
Ef fl uent
Reactor #1
16.0 ± 5.0
0.016 ± 0.021
13 ± 5
27
<0.10
<0.02
22
Effluent
Reactor #2
16.7 ± 4.3
0.014 ± 0.016
14 ± 10
31
<0.10
<0.02
21
Number of
Data Points
21
14
16
10
4
8
11
The purpose of Test A was to demonstrate that the two reactors performed in a
similar manner prior to beginning the carbon/copper addition tests.

Sludge age - 12 days, both reactors
Length of test - 21 days (after an 11 day start-up period)
Carbon dosage - None to either reactor
Cupric Chloride dosage - None to either reactor
                                     48

-------
activity of the two units.   The first statistical  test was comparing the cya-
nide levels of Reactors 1 and 2.   The following  t-test was used for the paired
observation tests:

                                    Mn -  E(Mn)
                              T  =  -2 -  D                       (5)
                                      est aMD


where:

     T  = is the 't1  statistic with n-1 degrees  of freedom

     n  =  is the sample size

    M   =  Z(Y  - Y.  )
   Y.   =  Observed value of the ith sample from reactor 1


 E(Mg)  =  Expected difference in population means
est aMD =  unbiased standard deviation of MD


     With the aid of this test, various hypotheses were formulated and  the
confidence levels of rejection and/or acceptance determined.   Using the 10%
confidence level for rejecting the hypothesis, the similarity of the two units
was tested.  The statistical results from Test A for cyanide  and BOD5 removals
are presented in Table 12.  Statistical analyses were limited to the cyanide
and BOD results because of the small number of samples for the other parame-
ters.  However, from Table 12, the results indicate that the  effluent cyanide
and BOD levels from the two reactors are indeed very similar.  Thus, even
though there was wide dispersion around each sample mean, the performance of
the two reactors was considered to be the same based upon the statistical
analysis.

TEST B - LOW CARBON/LOW COPPER TEST

     The first test for evaluating the PAC/CuCl2 system utilized a low carbon
and copper dose.  Then, in subsequent tests the carbon and/or copper dosages
could be increased and the new test initiated quickly.  This  technique mini-
mized the time interval between tests since several complete turnovers of
solids would be necessary to flush high concentrations out of the system.

     Cyanide removal through just a biological system was shown to be greater
than 90% in Test A.  Therefore, the apparent cyanide loading on the activated
carbon was expected to be quite small.  Apparent loading used in this context
is considered the difference in cyanide removed between the control and  test
unit divided by the carbon dosage.  A copper dose of 0.5 mg as Cu per liter
of feed was selected for Test B accompanied by a carbon dose of 14 mg per


                                      49

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tn
o
              Parameter
Effluent Cyanide levels

Effluent BOD,, levels
                                   TABLE 12.  TEST A - STATISTICAL RESULTS
                             Sample means rng/A
                               MI         M,
                                     0.0158    0.0139

                                    13.4      14.2
                                                           Hypothesis
MI  =  MZ

MI  =  M2
      Paired  observations and 10% significance level utilized.
                   'T'
                Statistic
0.45

0.63
             Hypothesis is
          accepted or rejected
Accepted

Accepted

-------
                    TABLE  13.  TEST B - SUMMARY RESULTS
Parameter
Flow rate, I/day
Cyanide, mg/A
BOD, mg/A
TOC, mg/A
Phenol , mg/A
Copper, mg/£
Suspended solids,
mg/fc
Inlet
_
0.31 ± 0.13
180 ± 180
145
57
<0.02
26
Effluent
reactor
n- Control
17.9 ± 3.8
0.017 ± 0.010
16 ± 11
24
<0.10
<0.02
8
Effluent
reactor
#2-Test
18.4 ± 2.8
0.020 ± 0.010
30 ± 13
25
<0.10
0.15
15
Number
of data
Points
19
19
13
8
6
8
9
Sludge age - 12 days, both reactors
Length of test - 19 days (at start of test, slugged dosed  the  aeration  basin
  of reactor #2 with 2.5 gm Carbon plus 9.5 mg Cu.)
Carbon dosage - Reactor #2 only - 14 mg/fc
Cupric Chloride - Reactor #2 only - 0.5 mg as Cu/fc dosage
                                    51

-------
 liter of feed.  The sludge age was maintained at 12 days, thus allowing a
 theoretical PAC concentration of approximately 250 mg/£ in the aeration basin
 of the test reactor.

     At the beginning of Test B, the test reactor (#2) was slug-dosed with
 2,500 mg of carbon (lignite type) and 9.5 mg of copper to bring the operating
 conditions close to the design levels.  Test B was then carried out over a
 19 day period.  A summary of performance results during Test B is presented
 in Table 12.  The BOD5 removal across the test reactor was 89% as compared to
 94% for the control reactor.  The difference in BOD5 removal efficiency was
 attributed to the initial slug-dose of copper and perhaps carbon.  The BODs
 removal dropped off the first day in the test unit and never recovered during
 this test interval.  In retrospect, the carbon and copper should have been
 added gradually over several days so as to not upset the system.

     The results of Test B didnot indicate any substantial advantage of the
 test unit over the control.  Cyanide removal across the test reactor averaged
 93.5% as compared to 94.5% across the control unit.  Comparing the total quan-
 tity of cyanide removed across both reactors (taking into account the slightly
 different flow rates), yields of 5.34 mg/day for the test unit and 5.24 mg/day
 for the control unit were obtained.  Thus, the apparent loading on the carbon
 was the following:

                  5.34 mg CN"  5.24 mg CN-
                  	day    	day    _  0.37 mg CN"
                      0.250 qm Carbon       ~   gm Carbon            (6)
                              day

     This apparent loading was quite low when compared to the batch test load-
 ings of typically 1.5 - 2.0 mg CN~/gm Carbon.  The results from Test B indi-
 cate that because of the competitive adsorption between the organics and the
 cyanide for the active carbon sites greater quantitites of PAC would be re-
 quired to satisfactorily remove the cyanides.

     By comparing the effluents from the two reactors in a statistical  manner,
 the effect of the PAC/CuCl2 system can be determined.  In Table 14 the sta-
 tistical  test results are summarized.  The cyanide removal efficiency of the
 control  and test unit appears to be the same in this test.  The means are
 close together, and allowing for the standard deviation and analytical  preci-
 sion these means cannot be considered distinctive.  Thus, on a statistical
 basis, no additional  cyanide removal could be attributed to the PAC/CuCl2
 addition.   The slug dose of copper did significantly reduce biological  activ-
 ity, and in Table 14 the mean effluent BOD from the control is significantly
 better at the 10% confidence level.  Thus, toxic effects of copper occurred
when copper amounts were slug-dosed and the biological system did not recover.

TEST C - HIGH CARBON/LOW COPPER TEST

     The results of Test B suggested that a higher carbon dosage would be nec-
 essary to improve the cyanide removal.  At the end of Test B, the carbon dos-
 age was increased from 250 mg/day to 750 mg/day, or an average of 39 mg carbon
 per liter of wastewater.  The sludge age was also increased from 12 days to 15

                                     52

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                                  TABLE 14.  TEST B - STATISTICAL RESULTS
Parameter
Effluent Cyanide levels
Effluent BOD levels
Sample mean, mg/H
MI Ma
0.0167 0.0196
16.5 30.1
Hypothesis
MI = M2
MI = M2
T
Statistic
-1.65
5.85
Hypothesis is
accepted or rejected
Accepted
Rejected
en
      Paired observations  and 10% significance level  utilized.

-------
days to allow a longer holding time for cyanide oxidation on the carbon.
This resulted in an equilibrium carbon level in the aeration basin of approx-
imately 1000 mg/& for Test C.  After a 2-day period for acclimation of the
system to the higher carbon make-up rate, Test C was initiated and continued
for a 14-day period.

     The results of Test C are summarized in Table 15.  Unlike the previous
test, no detrimental effects on the organic removal (BOD and TOC) were ob-
served in the test reactor.  The mean effluent copper level was also lower in
Test C (0.10 mg/£) as compared to Test B (0.15 mg/£), and this was considered
the result of the higher carbon level in Test C.  The PAC/CuCl2 addition re-
duced the mean effluent cyanide level over the control unit by 0.019 mg/Jl, or
28%.  The apparent cyanide loading for Test C is computed below:

               11.63 mg CM"   11.39 mq CN"
               	day    	 day   _ 0.32 mg CN"
                   0.750 gm Carbon         ~  gm Carbon               (7)
                           day

     This apparent loading is lower than the 0.37 mg CN~/gm carbon determined
for Test B, as would be expected.  (Removal efficiency increases as the load-
ing on carbon decreases in a completely mixed system.)  As compared to the
loadings reported in the batch tests (1.5 - 2.0 mg CN~/gm carbon), the load-
ing in Test C indicates that competitive adsorption by the organics is a real
phenomenon that consumes most of the active sites on the carbon.  However,
sufficient carbon was available for the removal of an additional 0.019 mg/S,
cyanide over the control reactor.

     The statistical analyses confirm the influence of the PAC/CuCl2 addition.
In Table 16 the computed statistical results are summarized.  That the PAC/
CuCl2 system improved cyanide removal over the control can be stated at the
2% significance level.  At the 10% significance level, the effluent cyanide
was reduced at least an incremental 0.01 mg/fc due to the addition of the PAC/
CuCl2.   Thus, the increase in carbon concentration improved the cyanide remo-
val.

     Linear regression analyses were performed for the test and control units,
comparing the effluent cyanide level to the inlet cyanide concentration.  The
results from both the test and control reactors were combined, and a dummy
variable utilized for the use of carbon/CuC!2.  The following equation is the
result of this technique.

               [Effluent CIT] = 0.14 [Inlet CN~] - 0.019 [PAC]

                T-Statistic     (2.84)          (-2.81)              (8)

where:

     PAC = 1 for test reactor

         = 0 for control reactor
                                     54

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                      TABLE 15.   TEST C -  SUMMARY TABLE
Parameter
Flow rate, I/day
Cyanide, mg/fc
BOD, mg/H
TOC, mg/£
Phenol , mg/£
Copper, mg/A
Suspended solids, mg/H
Inlet
_
0.66 ± 0.071
380 ± 90
130
80
<0.02
10
Effluent
Reactor #1
19.2 ± 3.8
0.067 ± 0.024
27 ± 9
27
<0.10
<0.02
10
Effluent
Reactor #2
19.0 ± 3.7
0.048 ± 0.016
28 ± 7
26
<0.10
0.10
10
Sludge age - 15 days
Length of test - 14 days (after a 2 day transition from Test B)
Carbon dosage - Reactor #2 only - 39 mg/A
Cupric chloride dosage - Reactor 12 only - 0.5 mg as Cu/A
                                     55

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en
     Parameter
Effluent Cyanide levels
Effluent Cyanide levels
Effluent BOD levels
                                    TABLE 16.  TEST C - STATISTICAL RESULTS
Sample means, mg/H
  Mi          M2
 0.0670      0.0478
 0.0670      0.0478
26.7        27.6
 Hypothesis
 —
  Mi  =  M2
M2 + 0.011 <
  Mi  =  M2
     Paired observations and 10% significance level  utilized.
                                                                            'T1
                                                                         Statistic
                                                                            3.01
                                                                            1.28
                                                                           -0.408
   Hypothesis is
accepted or rejected
      Rejected
      Accepted
      Accepted

-------
Both parameters, the inlet cyanide concentrations  and  the presence of the PAC/
CuCl2 are significant factors  at the  1%  level.   The  R2  (adjusted) for this
regression was 0.34, which indicates  that  there  are  other variables not includ-
ed in this regression that influence  effluent  cyanide  concentration, and these
other variables are discussed  in a latter  section.   Basically,  this statisti-
cal regression illustrates the importance  of PAC/CuCl2  in removing cyanide,
holding all other operational  variables  constant.

     The significance of the PAC/CuCl2  is  depicted graphically  in Figure 13.
The lines are separated by the difference  in the effluent cyanide means,
0.019 mg/A which is the most probable difference.   Because  of the use of the
dummy variable to indicate the effects  of  PAC/CuC12» the lines  in Figure 13
are parallel.  Not enough data were collected to discern the true relative
slopes of the two lines; although carbon adsorption theory  would predict that
the lines would converge at the lower inlet cyanide concentrations.

     The various statistical analyses of the results from Test  C clearly show
that the addition of PAC and CuCl2 into a  biological wastewater treatment
system significantly improves  cyanide removal.  The results also indicate  that
there was no deterioration in  the removal  of other organics (as measured by
the BOD and TOC tests) as a result of the  PAC/CuCl2 addition.  The  average
cyanide removal of 28% over the control  reactor was achieved with a  relatively
high carbon level and a low copper level.   In the following test, the  copper
dose was increased to ascertain if cyanide removal could be further  enhanced
via more copper.

TEST D - HIGH CARBON/HIGH COPPER TEST

     The final test with the single stage  activated sludge  units utilized  a
high carbon and copper dosage to determine the long term effects of the PAC/
CuCl2 addition.  The copper level for this test was increased to 1.0 mg/&  for
a  feed rate of 19 mg of copper per day.  The carbon level was maintained  at
the same level as the previous test, 750 mg per day for a dosage of 38 mg  of
carbon per liter of wastewater.

     The transition from Test C to Test D was accomplished by allowing the
test reactor  to adjust to the higher copper feed rate for one day.   After  this
acclimation period  Test D was initiated and lasted  for 43 days.  Any potential
detrimental effects of PAC/CuCl2 addition to the activated sludge system were
expected to be  evident by the end of the 43-day test.

     During the  first 30 days of Test D the sludge  age was maintained at 15
days after which it was reduced to 10 days  for  the  remainder of the test.
The  reason for  the  change in  sludge age is  included with the results.

     In  Test  C  with  the lower copper dose,  the  PAC/CuCl2 addition reduced the
mean cyanide  effluent level by  28% over the control.   In Test  D the copper
dose was  double  that  utilized in Test C, which  based  upon  the  batch tests,
should  have  resulted  in effluent  cyanide levels over  50% lower  than the con-
trol reactor.   The  results  of Test D, summarized  in Table  12,  show that the
test reactor  effluent cyanide level averaged  only  9%  lower than the control


                                      57

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W>
-o
•H
    0.12 -



    0.11 -



    0.10 -



    0.09 .



    0.08 .



    0.07 .



    0.06 .
oi   0.05
3
r-t
M-l

w   0.04
0.03



0.02 -



0.01 -
                                    vith PAC/CuCl2
                     Effluent  =0.14 [Inlet CN ~] -.01 91 PAC]
                     Cyanide
                     T-Statistic(2.84)
                                                  (-2.81)
                     where  PAC =
                                  1 for test unit

                                  0 for control unit
                0.4
                            0.5
                                  0.6
0.7
0.8
0.9
                          Inlet Cyanide, mg/2.
Figure 13.   Test C-Predicted effect of PAC on effluent cyanide levels.
                                   58

-------
reactor during the 43 day test.   In addition to the cyanide  removal  being
lower than expected, the dampening effect observed in Test C in  the  effluent
cyanide variation as a result of the carbon addition was  not apparent  in this
test.  The standard deviation of the test unit effluent cyanide  level  during
Test C was 0.016 mg/£ as compared to 0.024 mg/Jl in the control reactor.  In
Test D, the standard deviations  were 0.051 and 0.034 mg/fc for the  test unit
and control unit effluent cyanide levels, respectively.  Frequency distribu-
tions for the effluent cyanide levels, shown in Figure 14, also  reflect this
greater variation in the test units's effluent.

     Also contained in the summary Table 17 are the other chemical character-
istics used to describe the effluent and monitored during Test D.   The BOD
removal was improved by the addition of PAC/CuCl2 by 26%  over the  control  unit.
This effect on BOD removal is graphically depicted in Figure 15.  The larger
mean effluent COD and TOC concentration from the test reactor could be attri-
buted to the carbon fines in the effluent, as all of the  analyses  were per-
formed on unfiltered samples.  The removal of over 99% of the phenol across
both reactors continued as in previous tests.

     The test reactor effluent copper level averaged 0.20 mg/A indicating  that
80% of the copper was being adsorbed onto the carbon and  removed from the
system in the waste sludge.

Cyanide Removal Performance

     Analysis of the effluent cyanide concentrations from the two reactors
indicate that twice during Test D major performance changes  occurred in the
test reactor.  These changes in operating performance divide Test D into 3
intervals, which for discussion purposes, are designated Dl, D2, and D3.

     The change in effluent cyanide concentration with time is presented for
the test and control reactor in Figure 16.   Interestingly enough the inlet
cyanide concentrations  illustrated in Figure 17 do not indicate this varia-
tion from  period to period.  The effluent characteristics of the reactors in
Test D are depicted by  time intervals in Table 18, and the  performance in
each interval is discussed herein.

     The first phase, Dl, lasted for  16 days, during which  time the control
reactor effluent cyanide  averaged  0.073 mg/SL compared  to 0.045 mg/A for the
test reactor.  The  PAC/CuCl2 addition thus  resulted  in a mean cyanide level
38%  lower  than the  control reactor during the  first  16 days of Test D.  The
17th day of the test marked the beginning of a 2 week  period during which the
test reactor  performed  poorly compared to the  control  unit.  The  control unit
effluent during this  second phase, D2, averaged  18%  less cyanide  with a mean
effluent cyanide concentration of  0.115 mg/A while  the test unit  averaged
0.140 mg/Jl.   After  30 days the sludge wastage  rate  was increased  (sludge age
decreased) in an attempt  to reduce the cyanide equilibrium  concentration in
solution by lowering  the  total quantity  of  cyanide  maintained in  the  aeration
tank.
                                      59

-------
i't •
12 -
U)
01
c 10-
(U
u
M
3 0
o o •
u
O
^ 6 -
 ^ CD H-" 1— • 1— •
o ro f^
ii i i i i i







test




uni


t


Mean 0.091 tug
Std. Dev. ±.0


I 1
            .025  .050 .073 .100  .125 .150.175 .200.225 .250.275
                            Cyanide, mg/1
Figure 14.  Test D-Cyan1de frequency distributions,
                               60

-------
  130-1
   120
^  1101

60
6

 - 100-
s~\
 in
Q

§   90
-a
 C
 m
 E
 Q)
Q
 CJ
 bO
 ^
 X
 o
 u
 •H
 6
 QJ
 (U


 •r-l
80-
    70-
60-
50-
 u
 .2  ^o
    30
    20
    10
                 O -  Control  Unit

                 A -  Test Unit,
                     25            50             75

              Percent of Time Less  Than or  Equal To
                                                         100
    Figure 15.  Effect of powdered carbon on BOD5  removal-Test  D.


                               61

-------
 c
 o
 c
 -,
u
     0.20
     0.15
0.10
0.05 •
           o- Control

           A- Test
10
.* m _._.. —
20 30 40 1
Time , Days
)•*• 	 DJ »|
           Figure 16.  Test D-Effluent cyanide levels with  time.
                                   62

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    1.0 .,




    0.9 -





    0.6 '

i—i


e   0.7 '
•2   0.6 1
u
CO
Wi

c   0.5 1
a
o



3   0.4 H
    0.3 •
U
    0.2 -




    0.1

-*- 	 Dl
10

20


D2
30 40



                                  Time,  days
          Figure 17.  Test D-Inlet cyanide level with time.
                                 63

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                       TABLE 17.  TEST D - SUMMARY TABLE
Parameter
Flow rate, I/day
Cyanide, mg/£
BOD, mg/£
COD, mgA1
TOC, mg/a
Phenol, mg/H
Copper, mg/£
Suspended solids,
mg/A
Inlet
•
0.56 ± 0.12
280 ± 110
262
111
71
<0.02
24
Effluent
reactor #1
19.4 ± 2.2
0.100 ± 0.034
38 ± 20
70
24
<0.10
<0.02
10 ± 3
Effluent
reactor #2
19.5 ± 2.3
0.091 ± 0.051
28 ± 21
84
29
<0.10
0.20
13.5 ± 10
Number
of
Samp! es
tt
41
23
9
17
4
19
19
Sludge Age - 15 days for first 30 days, sludge age 10 days for last 13 days
Length of Test - 43 days (after a 1 day transition from Test C)
Carbon dosage - Reactor #2 only - 38 mg/Jl
Cupric Chloride dosage - Reactor #2 only - 1.0 mg as Cu

1 COD samples taken only during the last 18 days of Test D.
                                    64

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             TABLE 18.  TEST D - BY PERIODS - SUMMARY TABLE


Parameter
Cyanide, mg/H
BOD, mg/fc
TOC, mg/£
Suspended solids,
mg/A

Parameter
Cyanide, mg/&
BOD, mg/H
TOC, mg/fc
Suspended solids,

Parameter
Cyanide, mg/&
BOD, mg/fc
TOC, mg/H
#1 - Days
Inlet
0.61 ± 0.1
330
106
13
#2 - Days
Inlet
#1-16, Sludge Age -
Effluent
reactor #1
1 0.073 ± 0.022
34
24
10
#17-31, Sludge Age -
Effluent
reactor #1
0.56 ± 0.11 0.115 ± 0.018
270
141
22
#3 - Days
Inlet
0.51 ± 0.
220
74
37
28
10
#32-43, Sludge Age -
Effluent
reactor #1
11 0.123 ± 0.038
49
19
15 days
Effluent
reactor #2
0.045 ± 0.017
34
23
9
15 days
Effluent
reactor #2
0.140 ± 0.028
15
38
22
• 10 days
Effluent
reactor #2
0.097 ± 0.048
40
21

Number
of
sampl es
16
9
5
7

Number
of
sampl es
15
9
7
6

Number
of
sampl es
11
5
5
Suspended solids,
                        38
27
12
                                      65

-------
     After 31 days the test reactor effluent cyanide concentration decreased
rapidly and remained lower than the control for the remainder of the test.
During this period, D3, the test reactor effluent cyanide level  averaged 0.097
mg/fc and the control unit averaged 0.123 mg/Jl for a 26% improvement attributed
to the PAC/CuCl2.  Frequency distributions of the effluent cyanide concentra-
tions for the three phases of Test D are illustrated in Figure 18.  The fre-
quency distributions in Figure 18 show the increasing effluent cyanide concen-
tration from the control  unit with time.  (This same trend was also observed
in Figure 16.)  An analysis of these phenomena is discussed later in this
section.

BOD Removal Performance

     Daring Test D the BOD level was also monitored in the inlet and both ef-
fluents.  The variation in the BOD concentrations with time are presented in
Figures 19 and 20 for the effluents and inlet, respectively.  The first phase
consisted of steadily rising BOD's in the effluents whereas the inlet BOD was
actually dropping during  this period.  After the first 10 days of the test the
inlet BOD concentration rapidly increased but due to analytical  difficulties
(contaminated dilution water), no BOD data for the last 6 days of this period
were obtained.

     Although the BOD data are extreme1/ limited near the end of Dl, and the
beginning of D2, Figure 20 indicates that the organic loading was considerably
higher than during the rest of Test D.  A higher organic loading could result
in the displacement of some adsorbed cyanide on the carbon due to the higher
driving force of the organics.  While the data are too limited to draw any
conclusions concerning the reason for the higher cyanide levels from the test
reactor during D2, the higher organic loading during the later portion of Dl
is a plausible explanation.  One other interesting observations from Figure 19
is the lower effluent BOD's during period D2 in the test reactor effluent.
Whether this is related to the higher effluent cyanide levels during the same
period could not be determined.  Although the test reactor effluent BOD was
significantly lower than  the control reactor during period D2, (59% lower),
the total  organic carbon  results did not exhibit the same trend.  The TOC
results are presented in  Figure 21.

Statistical Analysis

     The statistical analyses of Test D for each time period are summarized in
Table 19.   The effluent cyanide concentrations for all of Test D were signi-
ficantly lower in the test reactor than in the control reactor and so were
the effluent BOD levels.   To further clarify the results of Test D statisti-
cal analyses of the results during each period were also performed, as summar-
ized in Table 19.

     During period Dl, the effluent cyanide level from the test reactor was
consistently lower than the control, and this difference in performance was
verified statistically at the 10% significance level.  In period D2 an
increase in the test unit's effluent cyanide level  occurred which resulted
in higher cyanide concentrations for the test unit rather than the control.


                                     66

-------
Number of Occurrences
0 V f 7* J» 5

MH


^•M
101
Mean 0.073 mg/i
Std Dev. ± .022 mg/i
8 •
in
«
V
i_
u-
0





'°1
Mean 0.11 A mg/«.
Std Dev. ± .018 mg/i ^
in
u
S 6-
t_
u
<4-
o
E
z o


Mean 0.120 mg/i
Std Dev. ± .038 mg/i
Tfti
             Cyanide, mg/Jl


       Control  Unit  during Test D-1
       Cyanide,  mg/i


Control  Unit during Test D-2
0.00  0.05   0.10  0.15  0.20  0.25

           Cyanide, mg/i


       Control Unit during Test  D-3
in
a>
u
c
t)
i.
8<
6.

4-
2l

0
0




w«i


.00
^^^






0.
'
Mean 0.045 mg/i
Atd Dev. ± .017 mg/i g.
4)
1.
0
"1 u 2-
4)
_^ ^i
1 ' °
05 0.10 0.15 0.20 0.25 c







1.00 0.05 0.



«••



10







0.
10-
Mean 0.139 mg/i
Std Dev. ± .028 mg/i
8-
i/i
4>
|6-
3
f
o
i 2-
-g
z 0
15 0.20 0.25 0
Mean 0.094 mg/i
Std Dev. ± .048 mg/Jl





"1
.00 0.05 0.10 0.15 0.20 0.25
              Cyanide, mg/H


          Test  Unit during Test D-l
       Cyanide, mg/i


   Test Unit during Test D-2
       Effluent Cyanide,  mg/i


     Test Unit during Test  D-3
                            Figure  18.  Test  D  - Cyanide frequency distributions by  period.

-------
   100 -i


    90 •

    80 -

    70 •

    60 -


    50
Q
§   40  -
    30 -


    20 '

    10 '
                   10
                Dl
            o -  Control
              -  Test
—i—
 20
30
                                     D2
                                 Time,  days
—i—
 40
                     D3
          Figure 19.  Test D-Effleunt BOD5 with time.
                                  68

-------
    600
    500 •
    400 -
cp
£
g°  300  I
    200  -
   100  -
	 1 	 1 	 1 — — 	 	 , 	
10
-, Dl »u

20 30 40
0
*"

•* D3 	 *-


                                   Time, days
            Figure 20.   Test  D-Inlet BODS with time.
                                  69

-------
o
H
   140
   120
   100
    80
    60-
    20 -
                    10
               Dl
20
30
                                  Time,  days
40
         Figure  21.  Test D-Inlet and effluent TOC with time.
                                  70

-------
                               TABLE 19.  TEST D - STATISTICAL RESULTS
Parameter
Effluent Cyanide - D
Effluent BOD - D
Effluent Cyanide 0-1
Effluent Cyanide D-l
Effluent Cyanide D-2
Effluent Cyanide D-3
Sample means, mg/£
Ml Ma
0.0997 0.0905
38.3 27.6
0.0726 0.0452
0.0726 0.0452
0.1142 0.1392
0.122 0.097
Hypothesis
MI = Ma
MI = Ma
MI = Ma
M2 + 0.023£Mi
MI = Ma
Ma = MI
T
Statistic
2.29
2.45
7.85
1.25
-2.50
4.41
Hypothesis is
accepted or rejected
Rejected
Rejected
Rejected
Accepted
Rejected
Rejected
Paired observations and 10% significance level  utilized.

-------
The performance difference was statistically analyzed and the two means were
significantly different at the 10% level.  During the last test interval, D3,
the removal efficiencies of the two reactors  reversed again  with  the  bet-
ter performance being achieved in the test unit.  The statistical test used
indicated that this performance change was significant at the 10% level.

     Thus, it can be confirmed with some statistical support that there occur-
red significant differences in performance both between the two units, and
between the different time periods.  However it should be noted that  the
sample dispersion and sample size both influence the validity of the  conclu-
sions offered and should be considered limitations to the analysis.

Discussion of Test D

     The phenomenon of decreasing removal efficiency was one which was ob-
served not only in Test D but throughout the continuous tests.  The control
reactor exhibited a generally decreasing cyanide removal efficiency during
the course of these tests as summarized below in Table 20.

            TABLE 20.  CYANIDE REMOVAL ACROSS THE CONTROL REACTOR

Test
A
B
C
Dl
D2
D3
Mean inlet
cyanide concentration, mg/£
0.17
0.31
0.66
0.61
0.56
0.50
% Cyanide
removed
91
94
90
88
80
76

     A satisfactory explanation for the decreasing removal efficiency in the
control could not be readily determined.  The change with time could be attri-
buted to a gradually increasing fraction of complex cyanides in the feed.  The
possibility of an increasing fraction of complex cyanides in the refinery
wastewater arises due to the greater demand for gasoline during the summer
months.  As the summer approaches, refineries generally increase their feed
rates and cracking severity in the cracking units.  The very severe cracking
conditions promote the formation of more cyanide.  The more cyanide produced,
the more corrosion that will occur, and this in turn effects the quantity of
iron cyanide generated.  The sour water stripper removes all but a small frac-
tion of the simple cyanides (stripping appears to be a concentration limiting
phenomena) and none of the iron cyanides.  Thus, one would expect a relatively
constant simple cyanide concentration in refinery wastewaters and increased
complex cyanide concentration during periods of maximum gasoline production.
Iron cyanide removals across biological treatment systems are lower than the


                                    72

-------
simple cyanide removals,  as  discussed in  Section  3.   The  postulated  higher
complex cyanide concentrations would  explain  the  reduced  cyanide  removal effi-
ciency across the control  reactor observed  with time.

     The increasing percentage of complex cyanides  in  the inlet would also
have an effect on the test unit due to the  difference  in  rates of oxidation
of simple and complex cyanides.  As explained in  Section  3,  the rate of oxi-
dation of complex cyanides has been found to  be much  slower  than  for simple
cyanides.  Thus, the test unit would  be affected  by an increasing equilibrium
cyanide concentration in  the carbon sytem.  As the  fraction  of complex cya-
nides in inlet increased, so would the amount adsorbed onto  the carbon, but
due to the slower oxidation  rate, more cyanide adsorbed on the carbon would
in turn increase the equilibrium cyanide  concentration remaining  in  solution
or in the effluent.

     A comparison of the  apparent cyanide loadings  on  the PAC for the various
tests is shown in Table 21.   This loading,  which  is defined  as the difference
between the quantity of cyanide removed in  the test and control units divided
by the carbon dosage, changed significantly during  the test  program. The
apparent loading at the beginning of  Test D was more  than 2% times greater
than that for Test C, even though the carbon  dose and inlet  cyanide  concentra-
tion were approximately the  same.  (The copper dosage for Test D  was twice
that of Test C, and this  accounts for the greater removal and thus higher
apparent loading.)  However, the apparent cyanide loading for Test Dl was
still below the 1.5 to 2.0 mg CN"/gm  carbon measured  in the  batch tests.

                     TABLE 21.  APPARENT  CARBON  LOADINGS

Carbon dose,
Test rng/A
B
C
Dl
D2
D3
14
39
38
38
38
Apparent loading,
mg CN~/gm carbon
0.37
0.32
0.80
(-0.
0.73



59)


     The results of Test D confirm the findings of the previous continuous
tests, that is, competitive adsorption from the organics reduces the quantity
of cyanide adsorbed by the PAC/CuCl2 addition.  The higher outlet cyanide lev-
els during period D2 were attributed to overloading of the carbon from high
organic levels in the feed, although the data are lacking to confirm this
statement.  To increase the quantity of cyanide removed, the following two
options are available:
                                     73

-------
     1.  Reduce the organic loadings

     2.  Increase the carbon dosage.

The economics of this process depend  almost entirely on  the carbon  dosage.
Instead of increasing the carbon dosage further,  the first  option of a  reduced
organic loading was evaluated in the  final  test.   Some refineries utilize a
two-stage biological treatment scheme.   By  adding the PAC/CuCl2  to  the  second-
stage, the organic loading on the carbon should be 50-80% lower  than In- a one-
stage system.  In the last continuous test, Test  E,  a two-stage  biological
treatment system was utilized to check the  hypothesis that  a lower  organic
loading should increase the quantity  of cyanide removed.

TEST E - LOWER ORGANIC LOADING WITH MEDIUM  CARBON/HIGH COPPER DOSAGE

     In order to evaluate the effect  of lower organic loadings on cyanide
removal, a two-stage activated sludge system was  used in Test E.  The effluent
from a common first stage was split into two streams; one for a  second-stage
control and the other a second-stage  test reactor.  In the  second-stage test
reactor a carbon dose of 25 mg/A, or  500 mg/day,  and a copper level  of 1.0
mg/5, or 19 mg (as Cu) per day were utilized.  The carbon and copper were both
added for twelve days prior to initiating Test E  to acclimate the micro-orga-
nisms and to build up the carbon level  in the aeration basin.

     The actual test period lasted 12 days  at a sludge age of 15 days.   As  in
the previous tests, the efficiency of the biological treatment process  was
monitored by measuring the BOD, COD,  and TOC levels in the influent and efflu-
ents.  The average performance characteristics of the test and control  reac-
tors are summarized in Table 22.

     As Table 22 indicates, the effluent cyanide concentration from the test
reactor was 53% lower than that of the control reactor.   The effluent cyanide
level from the control reactor averaged 0.141 mg/jl, as compared  to  the mean
inlet cyanide level of 0.140 mg/£.  The lack of any cyanide removal across  the
second stage control reactor supports the previous hypothesis that  primarily
simple cyanides are removed in the first stage activated sludge  unit, leaving
the stable complex cyanides for the second  stage.  As in Test C  and part of
Test D, the carbon addition dampened  the day to day cyanide variation as mea-
sured by the standard deviation, (0.024 mg/A to 0.060 mg/fc).  This  dampening
effect in the effluent cyanide level  is also evident in  Figure 22,  which
compares the two effluent cyanide levels with respect to time.

     The biological performances of the second-stage control and test reactor
also varied but to a much smaller extent.  The organic loading on the second-
stage, which averaged 82 mg/Jl BOD (unfiltered), 92 mg/A COD  (filtered), and
25 mg/  TOC  (filtered), was much lower than that for Tests A, B, C, and D.
The effluent from the test reactor contained, on the average, less BOD (6 mg/
A), COD (7 mg/£), and TOC  (1 mg/A) than the effluent from the control reactor.
However, the suspended solids in the test effluent were usually 5 mg/A higher
than the control effluent.
                                     74

-------
   0.250 1
                                       o  -  Control

                                          -  Test
   0.200 •
 ,  0.150

-------
                    TABLE 22.   TEST E - SUMMARY TABLE
Parameter
Flow rate, Jl/day
Cyanide, mgfi
BOD, mg/2.
COD, mg/sX1)
TOC, mg/J^1)
Copper
Suspended solids,
Inlet
-_-
0.140 ± 0.045
82
92
25
<0.02
—
Test
effluent
Reactor #3
20.0 ± 1.9
0.066 ± 0.024
21
43
10
0.20
17
Control
effluent
Reactor #4
20.2 ± 3.6
0.141 ± 0.060
27
50
11
<0.02
12
Sludge age - 15 days
Length of test - 12 days (after a 12 day acclimation period with carbon
   and copper added to system) .
Carbon dosage - Reactor #3 only - 25 mg/£
Cupric chloride dosage - Reactor #3 only 1.0 mg as Cu/£
       Test E only all  COD and TOC analyses were performed on filtered
   samples.
                                   76

-------
     Only a limited amount of data was collected during this  short  test,  and
to statistically evaluate the results, these data were pooled using a  dummy
variable for reactor type.  With 20 data points the difference in cyanide re-
moval efficiencies between reactors was considered statistically significant
at the 0.5% level.  Or, in other words, the probability that  the control  and
test reactor performed differently is greater than 0.995.   This can be ob-
served visually by inspecting Figure 23.  Not only are the means quite differ-
ent but also the dispersion and location of the frequency  distribution.

     The large reduction in cyanide (53%) attributed to the PAC/CuCl2  addition
tends to support the hypothesis that a lower organic loading  would  increase
the removal of cyanide.  The apparent cyanide loading (the difference  in  quan-
tity of cyanide removed by the test and control reactors,  divided by the  car-
bon dose) for Test E was 2.74 mg CN~ per gm carbon.  The apparent cyanide
loadings for all the one-stage tests ranged from 0.32 to 0.80 mg CN~ per  gram
of carbon (see Table 21).  Such a variation in cyanide loading clearly indi-
cates that lower organic loadings increased the quantity of cyanide removed
per gram of carbon.

SUMMARY OF PHASE II

     Continuous tests were performed over a four-month period to evaluate PAC/
CuCl2 addition into an activated sludge system for cyanide removal. Batch
tests, utilizing iron cyanides added to Chicago tap water, achieved cyanide
removals in excess of 95%.  The continuous tests were performed with actual
refinery wastewaters utilizing two reactors; one as a control  unit  and the
other for evaluating the PAC/CuCl2 system.  A series of five  tests  were per-
formed, which are listed below:

          Test A - no carbon - no copper
          Test B - low carbon - low copper
          Test C - high carbon - low copper
          Test D - high carbon - high copper
          Test E - medium carbon - high copper (two-stage  system)

     The results from these five tests demonstrated that activated  sludge
units remove as much as 90% of the cyanide present in refinery wastewaters.
The addition of PAC/CuCl2 directly into the aeration basin can remove  addi-
tional quantities of cyanide without any apparent effect on the micro-organ-
isms.  With the exception of the initial slug dose in Test B, no  inhibitory
effects on the biota were observed in the activated sludge performance.

     The addition of PAC may improve the removals of BOD,  COD, and  TOC.  While
a significant improvement in BOD removal was observed in the longest test
(Test D) as a result of the PAC addition, Tests B and C did not confirm this
trend and the data in Test E were too limited for analysis.  The  TOC and  COD
analysis were performed on unfiltered samples (except in Test E)  and the  car-
bon fines in the effluent samples contributed to both the  COD and  TOC  values
observed.  Again in Test E, the data were too limiting to  make any  definite
statement regarding the removals of COD and TOC.
                                     77

-------
   5-,
(/I
  /I
A)  ^
U

|


I  3
u
u
o
1-
OJ
                 Mean   0.066mg/2.

                    Std. Dev. ±  .024 mg/Jl
                        TEST UNIT
              0.050     0.100       0.150  '    0.200


                    Cyanide, mg/H
                                                          0.250
      Figure 23.  Test E-Effluent cyanide frequency distribution.
                              78

-------
     The organic loading was  found to  be  a  major  factor  in  the removal of
cyanide by the PAC/CuCl2 system.   Test E  supported  the hypothesis reached after
Test D, namely, decreasing the organic loading  improves  the cyanide removal
efficiency.  This is probably due to the  effect of  reducing the  competition
between the smaller amount of organics and  the  cyanide for  the available ad-
sorption sites on the carbon.  The increased availability of adsorption sites
to cyanide resulted in more cyanide being adsorbed  and,  therefore, correspond-
ing higher apparent cyanide loadings.   The  greater  apparent cyanide loading
of 2.74 mg CN" per gm of carbon,  is a  more  efficient  use of the  carbon, there-
by decreasing costs for the system. Therefore, the use  of  the PAC/CuCl2 system
is most attractive in a low organic load  waste  stream, such as in the  second-
stage of a two-stage activated sludge  system.
                                      79

-------
                                  SECTION 7

                 ECONOMIC IMPLICATIONS OF THE PAC/CuCl2  SYSTEM


     The technological  feasibility of utilizing the PAC/CuCl2  system in  an
activated sludge unit to remove cyanides has  been demonstrated by the results
of Phase II experimentaion.   Reduction of cyanides can be achieved;  however,
the competitive adsorption of organic compounds on the activated carbon  re-
duces the quantity of cyanide adsorbed per gram of carbon.   Without  prefer-
ential adsorption of the cyanides, this system will require larger carbon
dosages when the organic concentration is high.

     Cost estimates for operating this   PAC/CuCl2 system in a  refinery provide
additional  insight on its overall feasibility and applicability.  Based  on  the
data collected from the continuous tests in Phase II, which most nearly  simu-
lated actual refinery conditions, operating conditions of a PAC/CuCl2 system
are projected.  The costs of operating and installing such a system  are  then
developed for single-stage and two-stage activated sludge units.  A  comparison
of these costs with that of a granular carbon system emphasizes the  economic
advantages  derived from the PAC/CuCl2 treatment technique.   Thus, the operat-
ing range of economic feasibility is clearly demonstrated for  this experiment.

OPERATING CRITERIA FOR PAC/CuCl2 SYSTEMS

     The continuous tests of Phase II provided basic information regarding  the
operating performance of a PAC/CuCl2 cyanide removal system.  Important  cri-
teria in designing a system or estimating costs are the apparent cyanide load-
ing, existing effluent cyanide level, and copper dosage required. Data
gathered experimentally were used to describe these factors.

     To determine the quantity of carbon required to remove a  specified  amount
of cyanide, the apparent loading must be estimated.  The apparent cyanide
loading is  defined as the difference in cyanide removed between the  test and
control reactors, divided by the carbon dosage.  According to  adsorption the-
ory, the loading of a contaminant on carbon decreases as the carbon  dosage
increases.   Or, in other words, for each additional increment  of carbon  added,
a progressively smaller increment of the contaminant will be removed.  (This
can be readily seen in the batch test results, as shown previously in Figure
12.)

     To ascertain the effect of carbon dosage on apparent loading Tests  B and
C were both run at a constant copper dosage.   The apparent loadings  for  Tests
B and C, plotted in Figure 24, illustrates the effect of carbon dosage.


                                     80

-------
          50-,
00
          40-
       OJ
       CD
       (C
       o
       JD
       ea
       o
30.
          20.
          10.
1-stage
activat
sludge,
0.5 mg/
copper
                                 1-stage  activated
                                 sludge,  1  mg/2. copper
                     , B
                                                                          2-stage activated
                                                                          sludge, 1 mg/I copper
                      0.5

                                                      2.0
                                                      2.5
                                                                           3.0
                       Apparent loading,  mg CN /g Carbon
               Figure 24.  Effect of carbon dose on apparent loading.

-------
      From Figure 24,  increased carbon  dosage  had  only  a  slightly negative
 influence on the apparent cyanide loading.

      Also shown in Figure 24,  are extrapolated  loading lines  for the  conditions
 utilized in Test D and  E, under the  assumption  that  these  lines  would be par-
 allel  to the line passing through Test B  and  C.   The apparent cyanide loading
 is only slightly dependent on  carbon levels,  which means that the quantity of
 cyanide removed is approximately proportional to  the carbon dose.  Using Fig-
 ure 24, the quantitites  of carbon required  to achieve  specified  effluent
 levels can be computed  for both a one-stage and a two-stage activated sludge
 system.

      The copper concentration  to be  used  in a PAC/CuCl2  system was  specified
 at 1 mg/i as a result of the batch and continuous tests.   No  detrimental
 effect on the performance of the activated  sludge units were  found  at 1 mg/£
 copper; however,  the literature indicates that  above 1 mg/Jl,  the performance
 of the activated  sludge  units  may begin to  decrease.

 COST ESTIMATION OF PAC/CuCl2 SYSTEMS

      The costs of utilizing a  PAC/CuCl2 cyanide removal  system were based upon
 the apparent loading values of Figure  24 and  copper  concentration of  1 mg/5,
 with the goal  of  achieving an  effluent cyanide  level of 0.025 mg/i.   In Table
 23 the chemical costs are presented  for single-stage and two-stage  activated
 sludge units.   The chemical costs  were developed under the assumption that the
 PAC would  not be  recovered after use.   By eliminating  PAC  regeneration, this
 cyanide treatment method  can be  applied without any  capital expenditure or
 additional  plant  operators.  For larger carbon users regeneration would be
 more practical  and  economical.   Thus,  the estimated  chemical  costs  presented
 Table  23 are actually close to  the total cost of this  system.  Powdered carbon
 is  the primary cost of the system  (85-95%).   Thus the  required carbon dose
 determines  the economic  feasibility  of the  system.

     While  the PAC/CuCl2  process will  not solve the cyanide problem for all
 refineries,  this  process  has considerable potential   for some  refineries.  For
 refineries  with two-stage  activated  sludge  units operated in  series,  the costs
 as  shown  in  Table  23, are  approximately one-fourth of those for  a single-stage
 system.  To  place  the costs of operation of this proposed cyanide removal  sys-
 tem  in  perspective, a comparision with  another potential  system  is made in the
 following section.

 COST COMPARISION OF PAC VS 6AC CYANIDE  REMOVAL SYSTEMS

     The cost  advantages of the  PAC/CuCl2 system are most aptly  illustrated
 by comparison with other cyanide treatment methods.   As stated in Section 3,
all existing cyanide treatment methods  are considered expensive  by the refin-
 ing industry.  The economics of this  cyanide treatment system are compared
with the GAC catalytic oxidation system described in Section 3 as an example
of the projected cost savings to be realized.

     The GAC/CuCl2 system was investigated by one refinery in Illinois.12
Utilizing the refinery cost estimates and cyanide levels, a cost comparison

                                     82

-------
                  TABLE 23.   CHEMICAL COST SUMMARY
                       1-Stage Activated Sludge
                 To Achieve 0.025 mg/Jl Total  Cyanide
Present cyanide ef-
fluent concentration,
      mg/A
 Required carbon
     dose,
     mg/J,
  Chemical  cost
$/million gallons
      0.05

      0.10
      36

     108
        106

        286
                       2-Stage Activated Sludge
                  To Achieve 0.025 mg/fc Total Cyanide
Present cyanide ef-
fluent concentration,
Required carbon
    dose,
    mg/fc
   Chemical cost
 $/million gallons
      0.05

      0.10
     9.4

    28.0
          40

          86
Based upon 1 mg/SL CuCl2 (as Cu+2) and carbon cost of $.30/pound
and CuCl2 cost of $1.91/pound (as Cu+2).
                                 83

-------
 was prepared.   Placement  of granular  activated  sludge  system  (with CuCl2 and
 oxygen addition),  would bring  this  refinery  into  compliance with the Illinois
 effluent standard  of 0.025  rag/A.  Based  on pilot  plant data and an effluent
 flow of 4.2 MGD, the refinery  estimated  51,000  pounds  per day of carbon would
 be exhausted.   Assuming a carbon  regeneration furnace  is purchased and an
 attrition loss  of  10%, fresh carbon make-up  would  be 5,100 pounds per day.
 Ignoring the large capital  investment required  for the granular carbon system,
 the chemical  costs for both systems are  compared  in Table 24.

      Even with  the thermal  regeneration  of the  granular carbon, the make-up
 carbon alone would cost $3,000 a  day, as compared  with $210 or $810 per day
 for the powdered carbon cost without  any regeneration.  Taking into consider-
 ation the differences in  capital  investment, the  PAC system is even more
 advantageous.

      To provide some indication of  the range of operating costs expected,
 chemical  expenditures for the  other refineries  described in Table 1 were
 calculated.   The values of  Table  25 are  based on an effluent level of 0.025
 mg/i total  cyanide and a  copper concentration of 1.0 mg/£.  Depending upon
 the effluent characteristics of the refinery and the number of activated sludge
 stages,  the  costs  varied  from  $20,000 to $530,000  per year.  For any of the
 refineries  listed  in Table  25, the  costs are much  lower than the alternative
 cyanide treatment  methods.

 QUALITATIVE  ECONOMIC ASPECTS

      There are  other aspects of the PAC/CuCl2 system with offer economic ad-
 vantanges that  are more difficult to quantify.  The following list of benefits
 from the  addition  of PAC  into  an activated sludge  unit should reduce the oper-
 ating  costs  assessed to cyanide removal  even further:

      1.   The fluctuation  in  daily effluent cyanide levels is reduced,
          (except during periods of  high organic loadings).

     2.  Although  not observed in the laboratory activated sludge
         reactors,  PAC is reported  to lower effluent suspended
         solids.

     3.  Lower effluent BOD's are achieved.

     4.  Sludge dewatering characteristics are improved.

     While PAC/CuCl2 addition offers some economic advantages over other pro-
cesses, this process is not without its  drawbacks.  Where effluent cyanide
standards are based on daily maximums as opposed to monthly averages, the
value of this system is reduced.   As occurred in Test D,  slug doses  of high-
strength organic wastes can result in a  desorption of cyanide from the carbon,
resulting in significantly higher effluent cyanide levels.   Thus, where wide
fluctuations in the organic loading exist,  high  cyanide effluent peaks will
occur for one or more days.   Where a two-stage sludge system is  used, the
organic fluctuations should be reduced.


                                     84

-------
          TABLE  24.   CYANIDE TREATMENT - CHEMICAL COST COMPARISON


System
Granular carbon - with
thermal regeneration
Powdered carbon - no
regeneration
1-stage activated
sludge system1
Powdered carbon - no
regeneration
2-stage activated
sludge system2

Carbon cost*
$/day

3,000



810



210

Copper cost.
$/day

67



67



67
Total
Chemical cost,
$/day

3,067



877



277
Design Basis:
   Flow 4.2 MGD
   Existing cyanide effluent level  - 0.079 mg/fc
   Design cyanide effluent level  -  0.025 mg/£
   Granular activated carbon - $0.60/lb
   Powdered activated carbon - $0.30/lb
   Cupric chloride - $1.91/lb (as Cu+2)
   Apparent cyanide loading 0.7 mg CN"/gm carbon
   Apparent cyanide loading 2.7 mg CN"/gm carbon
                                   85

-------
          TABLE 25.  COST ESTIMATES FOR PAC/CuCl2 SYSTEM



Refinery
A
A
B
B
E
E
F
F
No. Of
stages 1n
activated
sludge
1
2
1
2
1
2
1
2

Effluent
flow rate,
MGD
2.6
2.6
4.2
4.2
2.0
2.0
6.8
6.8
Existing
effluent
cyanide,
rag/A
0.176
0.176
0.079
0.079
0.038
0.038
0.045
0.045
Annual
operating
costs,
$
530,000
150,000
320,000
100,000
46,000
20,000
220,000
86,000
Design basis:

  Cyanide effluent level  - 0.025 mg/A monthly average
  PAC - $.30/lb, CuCl2 -  $1.91/15 (as Cu+2)
  Apparent CN" Loadings:  1-stage - 0.7 mg CN'/gm carbon
                         2-stage - 2.7 mg CN'/gm carbon
                               86

-------
     In summary, the PAC/CuCl2 system has definite economic advantages  over
existing cyanide treatment methods available to the refining industry.   This
system is perhaps best suited for refineries requiring less than  a  60%  reduc-
tion in effluent cyanide levels and especially for refineries with  two-stage
activated sludge units.  Because there is no capital  expenditure  required  to
utilize the PAC/CuCl2 system, its use is extremely flexible.  For example,
some refineries experience high effluent cyanide levels for one to  two  months
after bringing the catalytic cracking unit back into operation after a  major
maintenance shutdown.  The PAC/CuCl2 system is Ideally suited for short-term
use in such instances where high cyanide levels are projected. As  discussed
in Section 3, many refineries also experience one to three months each  year
of very high effluent cyanide levels.  The PAC/CuCl2 system provides an econ-
omic approach to reducing these cyanide levels.  When the effluent  cyanide
levels are in compliance, no carbon or copper needs to be added.   For refin-
eries that require high degrees of cyanide reduction on a yearly  basis, Table
25 provides an indication that over $500,000 per year of powdered carbon may
be required.  While not included in this economic evaluation, PAC regeneration
by wet air oxidation would reduce the overall cost of treatment for the larger
PAC users.

-------
                                 REFERENCES


 1.  American Petroleum Institute, Report WBWC 3064, 1972 Sour Water Stripping
     Evaluation, Publication 927, Washington D.C., 1973, pp. 6, and 20-28.

 2.  Huff, L.L., and J.E. Huff.  Analysis of the Benefits and Costs of Alter-
     native Cyanide Standards in Illinois.  Illinois Institute for Environmen-
     tal Quality.  Report No. 75-24, 1975.

 3.  U.S. EPA, Quality Criteria for Water, 1976, p. 65.

 4.  Public Law 95-217, The Clean Water Act, Section 307 (a)(2), December,
     1977.

 5.  Environmental  Reporter.  McGraw-Hill Publishing Company, 1975.

 6.  Cyanide Destruction Using Ozone and Ultraviolet Light.  Mobil Oil
     Bimonthly Variance Report on Cyanide to the Illinois EPA, Springfield,
     Illinois, August, 1975.

 7.  Irvin, J.W., and H.D. Tomlinson.  Testimony presented in Regulatory
     Hearing R74-15, 16 before the Illinois Pollution Control Board, 1975.

 8.  Pettet, A.E.J., and E.V. Mills.  Biological Treatment of Cyanide With
     and Without Sewage.  J. Appl. Chem. 4:434-444, 1954.

 9.  Bucksteeg,  W.   Decontamination of Cyanide Wastes by Methods of Catalytic
     Oxidation and  Adsorption.  Presented at the 21st Annual Purdue Industrial
     Waste Conference, Purdue University, Lafayette, Indiana, May 3, 4, and 5,
     1966.

10.  Kuhn, R.G.   Process for Detoxification of Cyanide Containing Aqueous
     Solutions.   U.S. Patent 3,586,623, June 22, 1971.

11.  Bernardin,  F.E.  Cyanide Detoxification Using Adsorption and Catalytic
     Oxidation on Granular Activated Carbon.  JWPCF 45:221-231, 1973.

12.  Bernickas,  J.V.  Testimony presented in Regulatory Hearing R74-15, 16,
     before the  Illinois Pollution Control Board, 1975.

13.  Flynn, B.P., and L.T. Barry.  Finding a Home for the Carbon:  Aerator
     (Powdered)  or  Column (Granular).  Presented at the 31st Annual Purdue
     Industrial  Waste Conference, Purdue University, Lafayette, Indiana,
     May 1-3, 1973.

-------
14.   Adams, A.D.   Improving Activated  Sludge  Treatment with  Powdered Activated
     Carbon.  Presented at the 28th  Annual  Purdue  Industrial Waste  Conference,
     Purdue University, Lafayette,  Indiana, May 1-3,  1973.

15.   U.S. EPA.   Methods for Chemical Analysis of Water and Wastes.  1974.

16.   Sudo, R.,  and S.  Aiba.  Effect  of Copper and  Hexavalent Chromium  on the
     Specific Growth Rate of Ciliate Isolated from Activated Sludge.   Water
     Resources  7:1301, G.B., 1973.

17.   Poon, C.P.C., and K.H. Bhayani.   Metal Toxicity  to  Sewage  Organisms.
     Proceedings ASCE, J. Sanitary  Engr.  Division  97:S.A.2:161, 1973.

18.   Lamb, A.,  and E.L. Tollefson.   Toxic Effects  of  Cupric, Chromate  and
     Chromic Ions on Biological Oxidation.  Water  Resources  7:599,  G.B.,  1973.

19.   Salotto, B.V., E.F. Barth, W.E. Tolliver, and M.B.  Ettinger.   Organic
     Load and the Toxicity of Copper to the Activated Sludge Process.
     Presented at the 19th Purdue Industrial  Waste Conference,  Purdue  Univer-
     sity, Lafayette, Indiana, 1964.

20.   Barth, E.F., M.B.Ettinger, B.V. Salotto, and  G.N.  McDermott.   Summary
     Report on the Effect of Heavy Metals on  the Biological  Treatment
     Processes.  JWPCF 37:86, 1965.

21.   Winker. R.L., and W.L. Hays.  Statistics:  Probability, Inference, and
     Decision.  Holt, Rinehart and Winston, New York. 2nd Ed.,  1975.
                                     89

-------
                                               APPENDIX
                            TABLE A-l.  TABULATION OF ALL DATA FROM PHASE I.

Test &
Reactor
No.
1-1
-2
-3
2-1
-2
-3
3-1
-2
-3
4-1
-2
-3
5-1
-2
-3
-It
6

1-2
-3
-4
8-1
-2
-3
-It
9-2
-3
-4
10-1
-2
-3
-4
11-3
-4
12-3
-4
13-3
-4


6-hr CN
Cone. , mg/J.
< 0.01
0.027
0.060
< 0.01
0.01
0.05
0.30
< 0.01
<0.01
0.168
0.145
0.010
<0.01
0.040
0.010
0.010


Equilibrium
CN~ Cone. , mg/£
< 0.01
0.024
0.038
	
	
—
0.28
<0.01
0.013
0.120
0.115
< 0.01
< 0.01
0.025
0.010
<0.01

Carbon
Cone. ,
ng/*
250
250
250-
250
250
250
250
250
250
250
250
250
250
250
250
250

Type
of
Carbon1
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
H.D.
Initial
Copper
Cone . ,
mg/i
1.5
1.5
1.5
1.5
1.5
1.5
0.0
1.5
1.5
0.5
0.5
1.5
10.0
0.75
1.0
1.25

Initial
CN Cone . ,
mg/i
0.48
0.48
0.53
0.44
0.50
0.52
0.53
0.52
0.39
0.48
0.37
0.39
0.46
0.42
0.46
0.46

Type
of
CN"
Ferro
Ferro
Ferro
Ferri
Ferri
Ferri
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Filtrate
Copper
Cone, at 6 hr,
mg/i
1.8
0.05
0.05
1.7
0.05
0.02
< 0.02
0.10
0.10
0.05
0.05
0.15
0.4
0.05
0.05
0.05

Used in
Regression
Analysis
No2
Yes,
Yes
No3
No3
No3
Yes
Yes
Yes
Yes
Yes
Yes
No"
Yes
Yes
Yes
Test aborted after six hours, reactor split open;
repeated as Test 7.
0.16
0.12
0.05
0.23
0.46
0.39
0.023
0.29
0.29
0.015
_
0.32
0.50
0.26
0.29
0.15
._
—

—
0.025

0.13
0.11
0.39
0.29
0.023
0.12
0.16
0.01
0.41
0.26
0.45
0.27
0.30
0.10
0.09
0.24
0.63
0.51
100
250
1,000
0
100
250
1,000
100
250
1,000
0
250
100
100
250
250
100
100
0
0
H.D.
H.D.
H.D.
—
L.D.
L.D.
L.D.
L.D.
L.D.
L.D.
	
L.D.
H.D.
H.D.
H.D.
L.D.
H.D.
H.D.
	
—
1.0
1.0
1.0
0.5
0.5
0.5
0.5
1.0
1.0
1.0
0.0
1.5
0.5
1.5
1.0
1.0
1.0
0.5
0.0
0.5
0.34
0.44
0.38
0.53
0.53
0.66
0.57
0.41
0.67
0.50
0.67
0.69
0.66
0.70
0.63
0.67
0.40
0.41
0.66
0.65
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferro
Ferri
Ferri
Ferro
Ferro
Ferro
Ferro

0.05
0.05
<0.02
0.12
0.08
0.05
< 0.02
0.12
0.05
0.02
	
0.18
0.04
0.19
	
—
—
—
< 0.02
0.17

Yes
No5
No6
No7
Yes
Yes
Yes
Yes
Yes
Yes
No'
Yes
No8
No8
No3
No3
No8
No8
No'
No'
'H.D.  — High Density,  lignite-based;  L.D.  — Low Density,  lignin-based  carbon.
2pH too low,  did not have enough pH variation to  analyze  statistically.
3Ferricyanlde data not  used,  too few tests.
"Copper dosage beyond concentration range.
5Lost equilibrium cyanide sample during analysis  —  boiled  over.
'Equilibrium value appears erroneous,  does  not agree with 6-hr cyanide sample.
'Suspect reactor contaminated with carbon particles.
"Final data collected after regression analysis completed.
'Without carbon in the system, any removal  would  be  by a  different  mechanism.
                                                 90

-------
vo
                                                                       APPENDIX
                                         TABLE A-2.    DATA FROM  PHASE II  TEST  A  - BASELINE OPERATION

1
.*d
2-19
20
21
22
23

25
26
27
2S
2*
VI
2
3
4
5
6

7
ft

9
10
Hun

Cyanlda Cone.
titUt tfflu.nl 1 Effluent 2
0.23 0.059 0.063
--
— 1
0.19 0.067 0.012

0.21 0.020 0.020
0.15 O.OIS 0.012
-- " „
o.ii o.oo4 o.ooa
0.13 0.002 0.008
0.14 0.002 3.006
0.13 0.006 O.OOt
0.13 0.004 0.002
0.24 0.001 0.001

0.15 o.ooa O.OM
0.21 0.002 n.OU

0.20 0.01(1 0.026
0.19 0.010 0.012


WO
310 15 »
130 14 10
540 7 14
120 9 S

210 19 19
340 19 22
270 21 1*
360 16 16
10* 12 3
m » •
242 l> 10
168 9 10
219 19 23

127 11 U

130 « »
250 13 **


Total Organic Carbon
89 22 28
— —
91 37 31
	
106 24 27
84 27 25
—
6* 20 19
90 25 20
79 22 22
„

100 26 26

97 2) 23
III 27 31


Flow
	 I/day 	
19 24
24 17
II 14
12 13
165 22
21 16
18 14
15 22
15 1*
5 —
14 11
U U
11 12
16 14
17 21
19.5 18
18.5 19

8 11.5
23 22.5
23 22
16.0 16.7


Suspended Solid*
14 5
30 52
<5 <5
—
7 31 28
24 23
	
25 10
41 8 3
16 10


8 10

27 9 7
25 22 21


MLSS«».
I/I
2968 2805
2359 3013
2982 3159

2411 2717
2622 2377
	
2194 2126
1*31 1870
1795 1981


1667 1792

1642 1646
2257 2M9


Copper Cone.**
-./' .
„
._
— 	

__ — —
--
„
—
_-
.-


—

	

                 * loth r«»ctor« operated without Copper or PAC addition
                ** Copepr m>t Monitored during T«*t A
               .» Slud«. u. -..ted « . r.t. of 800 .l/d.y fro. «rh ..ration ba.ln

-------
                                                                          APPENDIX
                                     TABLE  A-3.   DATA  FROM  PHASE  II TEST B  LOW  CARBON/LOW COPPER*


(-11
12
1 1
14
15
16
17
IS
19
20
21
21
11
It
2}
11,
II
28
21
Neon

Cyan Ida Com- .
••»'•'•
11.21 0.008 0.016
o.iB 0.014 a.nin
n.17 0.006 O.O08
0.19 0.014 0.010
0.20 0.008 0.010
0.20 0.008 0.010
0.21 0.004 0.012
0.2) 0.006 0.012
0.021 0.012 0.010
0.027 0.008 0.012
0.049 0.026 0.020
0.62 0.028 0.0)7
0.11 0.022 0.028
0.1) 0.014 0.0)2
0.57 0.020 0.022
0.41 0.040 0.028
0.41 0.0)2 0.0211
0.40 0.020 0.0)7
0.)) 0.028 O.OM
O.JI 0.017 0.020

POD
=»/'
220 10 14
210 15 24
4iO 6 29
—
SO II 20
:.'4 IJ 2)
2)0 10 M
51(1 52 60
160 16 JO
—
2ID 17 22
„
190 11 48
-.
'50 14 )t
;>o 21 16
-.
j<» 14 ;-4
280 16.5 )0.«

Ti>La) Organic Carbon
08/1
97 23 2)
104 26 JO
~
-.
If 18 IB
..
„
4)0 :i 36
.-
—
154 )) 21.)
..
109 21 26.)
..
IJft 21. S 2*. )
..
„
112 11.5 21
145 24 25

Flov
^...'(X.... i
21.5 21.)
:2.5 17.5
16 20
18 18
18 18
» 9
17 21
8 20.)
19.) 19
18 17.9
17 18
17. J 17.)
Id.) 16.5
18 17.)
19 17
20 22
22 20
21 11
21.) 20
17.9 18.4

Suapendcd Solids
**/t
—
5 It
.-
.-
— 6 8
6 I)
7 2)
9 )7
.-
..
14 1 )
--
— 6 2
—
4 15
..
..
— 8 8
8 IS

KLSS"
mg/l
„
1861 16)3
_
_
2020 1964
1117 1MI
. 669 1207
1970 1196
—
_ _
1)73 14)9
--
1898 1719
_
2167 18)5
_
„
1705 1871
„

Copper Cone .
•8/1
<.OI <.OI 0.05
—
0.0) <.OI 0.20
-
<.OI <.01 0.16
„
«.01 <.01 0.18
„
<.OI <.01 0.1)
—
—
<.02 <.02 0.20
_
„
<.02 0.12
_ _
„
<.02 <.02 0.17
<.02 <.02 0.15
--I
VO
ro
      * Reactor I Is a mntrol with no copper or PAC

       functor 2 hA» 25O oft/dor PAC and O. 5 ««/•' • .-pp«r

       Sludge ngir 12 days In both rtvi-lorn


      •* Sludfif vnntcd nt a r..l<- ..f HCWl al/day fr<« hnth reart-

-------
                                                 APPENDIX
               TABLE A-4.   DATA  FROM PHASE  II TEST  C HIGH  CARBON/LOW  COPPER*
	 . 	 — — • 	


10
co
	 _ 	 1 	 	

Dace
4-1
•t
3
4
5
6
7
8
9
10
11
12
13
U
Mean

Cyanide Cone.
•c/t
tnl.t Effluent i Effluent 2
0.1.7 0.0*0 0.065
0.59 0.062 0.52
0.60 0.059 »-037
0.67 0.0*9 0.047
0.5J 0.062 0.04*
0.72 0.067 0.042
0.68 0.056 0.015
0.60 0.028 0.027
0.59 0.0*9 0.032
0.72 0.090 0.010
0.80 0.110 0.05«
0.72 0.103 0.065
0.69 0.096 0.056
0.65 0.067 0.081
0.66 0.067 0.0*8
	 , 	 : 	 • 	
	 . 	
BOD
«/l 	
Inlet Effluent 1 Effluent 2
390 29 32
336 16 18
273 13 23
500 30 30
550 35 37
305 *2 37
349 20 28
400 JO l«
120 25 25
380 27 2'
_— 	 	 	
Total Organic Carbon
M/t
170 30 10
121 23 25
117 30 29
136 35 36
124 28 22
110 1* '2
130 27 26
^__^— 	 —
Flov
8 H
21 17
20 20
18 22
15.5 15.5
18.5 19
19 20
20.5 19.5
21 21
22 22
22 2*
21 21
21.5 21.5
21.5 12.5
19.25 1»-0
Suspended Solids
»B/l
15 11
6 13
16 1*
13 13
7 6
3 4
10 10
MLSS**
•g/t
teactor 1 Reactor 2
2030 2054
1349 2149
1957 2812
1639 2878
1663 2492
1924 3166
^ — — ^ •

Copper Cone .
.g/t 	
Inlet Effluent 1 Effluent 2
<.02 0.15
<.02 0.12
<.02 «.02 
-------
                                                      APPENDIX
                           TABLE A-5.  DATA FROM PHASE II TEST D HIGH CARBON/HIGH COPPER*
to
-p.

Cyanide Cone. 1 |OD
•«/' 1 mt/t
"Bate ' Inlet Effluent T Effluent I — Inl«t — Effluent 1 — tffluent 1
r*rtod 1,
4-16 j 0.7* 0.0i2 0.028
17 0.74 0.0*9 O.OJO
18
19
20
21
22
23
2*
25
26
27
28
2*
30
5-1
Hun
reeled 2
5-2
3


10
II
12
1}
1*
IS
16
Nun
Period 3
J-17
18
0.5* 0.0** 0.024
O.S3 0.052 0.035
O.S3 0.032 0.022
0.53 O.O*5 0.030
0.57 0. IDS 0.0**
0.5* 0.08* 0.0*1
0.*9 0.067 0.0*9
0.67 0.070 0.0**
0.53 0.084 0.0*5
0.49 0.087 0.059
0.56 0.078 0.0*2
0.85 0.096 0.061
0.67 0.0«6 0.08*
0.72 0.100 0.0*9
0.61 0.073 0.0*5
0.37 0.090 0.127
0.41 0. 110 0.202
0.54 0.096 0.135
0.50 0.100 0.120
0.5* 0.170 0.110
0.47 0.100 0.1*0
0.3* 0.103 0.103
O.53 0.110 0.140
0.53 0.127 0.191
0.67 0.120 0.127
0.6! 0.100 o. 113
0. 70 0. 1 35 0. 160
0.70 0.1*0 0. U*
0.70 0.1*8 0. 127
0.56 0.11* 0.139
0.70 0.175 0.103
O.t-5 0.151 0.072
19 — — —
20 1 0.51 O.CM* 0.032
21 '
22
23
2*
25
26
27
0.51 0.116 0.0*2
0.5* O.O93 0.0*7
0.5* 0.106 0.093
0.32 0.100 0.08*
O.«0 0.191 0.135
O.*0 0.115 0.155
0.45 0.078 0.090
28 j 0.45 0.09ft 0.186
	 Ne.ii> 1 0.50 .-'.130 0.094
310 U 20
**0 22 1J

230 29 Tl
310 18 30
210 30 3]
230 45 30
2*0 19 37

—


580 83 97
*OO ** J3
S30 3* 3*
__
*20 50 31
220 «6 *
2*0 51 8
180 3* 12
320 60 21
—
II II II
330 24 9
280 1* 12
140 *0 18
320 16 17

272 37 15
II II II
100 55 5*
270 35 66
300 60 39
—
—
230 18 15
182 75 22
216 *8.6 39.6
M/t
Inlet — ('fluent 1 Effluent 2
120 25 2*. 5

106 25.5 20.5

86.6 25 29.5

99 ?., 21
-- ..

117 16 19.5
106 2* 23

7* 27 32
10* 11 U
137 23 tO
130 31.5 18

13* 20 39
127.5 28 37
283.5 3*. 5 46

1*1 28 38
-
11.5 14.5 17.5
70.1 U.I 12.*
-*
I-" 17.8 21
47.4 19.2 19.3
58 31 31
74.2 19.3 20. 2
Flow
I/day
I»»ctor 1 — K««ctor 2
21.5 21
21.5 21
21 19.5
22 19
14.5 15.5
19 21
18.5 21.5
18.5 20.5
19 22
18 19.5
19 20.5
21.5 21
21.5 20.5
20.5 21.5
19.5 21.5
20 20
19.7 20.3

17 18
17 16
16 16
18 t7
15 5 14 5
18 17
20 19
18 19
17.5 18
16 5 18
18 18
17.5 16.5
19.5 20
21 20
18 18
17.8 17.7
18 20
19.5 22
23.5 25
21.5 22
22° 21
23.5 23
23 18.5
22 22
2O 19
2* 2*
22 21
18 16
21.4 21.1
Suspended Solid*
6 4
—
6 5
—
7 5
7 4
« —
21 8
— —
9 7
13 28
99 9

12 1*
9 9
10 26
_„ 	
9 32
10 29
13 24

10.5 22.3
10 7
10 10
10 9

11 5
U 12
II 27
MLSS««
2019 3214
. __
1619 3160
—
2231 3312
1621 3O65
—
2051 3126
	
2027 2979
1368 1593
-_ .

1/38 1201
991 9OO
705 996

156O 2325
1390 2597
1110 1784
— — _
„
1780 27*0
7*1 1180
12*6 1960
« "
1797 1378
1707 1651
1172 2376
Copper Cone.
mm/ 1 	
l.l.t Effluent 1 Effluent 2
-• .02 0.08
«. .. —
<.02 0.12
—
<.02 <.02 O.I*
—
< .02 0.1*
	 	 „
„
<.02 0.18
<.02 <.02 0.13

<.02 0.28
<.02 <.02 0.26
<.02 0.29
—
<.02 0.28
<.02 <.02 0.28
<.02 0.28
—
<.02 <.02 0.28
<.02 — 0.16
<.02 <.02 0.15
<.02 <.02 0.18
__
<.02 0.19
<.02 <-02 0.20
<.02 
-------
                                                                   APPENDIX
                TABLE A-6.    DATA  FROM  PHASE  II  TEST E  TWO STAGE  TEST,  MEDIUM CARBON/HIGH  COPPER*


IW
5-30
31
6-01
2
3
4
5
6
7
1
9
10
11
Mean

Cyanide Con1:.
Inlet Effluent 3 Effluent *
0.087 0.087 0.197


0.0*0 0.0*4 0.22?
0.1SO 0.100 0. 1*8
0.180 0.103 0.151
0.113 0.062 0.127
0.180 0.062 0.170
0.081 0.0*4 0.191
0.130 0.032 0.0*0
0.155 0.056 0.070
0.202 0.065 0.087
0.1*0 0.066 0.1*1
BOD
Inlet Effluent 3 Effluent *
	 _„ 	

_
ISO 19 70
88 27 36
62 17 28
	 	
«7 13 17
41 8 10
97 IB 14
89 13 13
82 21 27
Total Organic Carbon

13 10 11
„_ 	 	
20 6 7
*2 10 10
40 10 11
„
—
17 12 1*
—
19 12 10
24 13 12
25 10 11
Flow
17 10
20 21
20 21
18 20
20 21
19 21
20 21
18 21
22 21
22 22
22 22
22 22
20 20
Suspended Solids

5 4.6
—
10 3.4
.-
* 5.4
—
—
* 5.*
~
6 5
6 6
5.8 5.0
MLSS"
	 -111 	

636 205
„
1156 2*3
—
494 238
—
—
646 21*
	 	
835 *2*
790 402
-
Copper Cone.
-/I
„
<.02 0.21 <.02
<.02
<.02 0.11 '-02
<.02 0.20 <-02
•c.02 0.23 <-02
""
__ —
<.02 0.19 '-02
~
<.02 0.11 ''02
0.35 <-02
<.02 0.20 «-02

tn
                •Reactor 4 1» a control unit with no copper or PAC added
                Reactor 3 has 500 «g/day PAC and 1.0 mg/l of copper added
                Sludge age was 15 days

               ••Sludge WHS wasted «t a rate of 650 at/day fro« both »er«tlon basins

-------
                                                    APPENDIX



                                     TABLE A-7.  BATCH TEST CYANIDE DECAY  DATA
Test and
Reactor No.
1-1
1-2
1-3
3-1
3-2
4-1
4-2
4-3
7-2
7-4
9-2
9-3
9-4

Initial
1.83
1.81
2.02
2.20
1.99
1.81
1.70
1.78
3.74
0.53
5.29
2.56
0.67
me
0.01 days
--
—
--
—
1.91
1.72
—
--
—
—
—
2.38
~ ™
of CN" per gram of Carbon at:
1 day
1.77
1.71
1.99
1.45
1.80
1.58
1.57
1.74
--
--
3.86
2.12
0.58
2 days
1.01
1.33
1.67
1.33
1.77
1.68
1.57
1.74
2.95
0.45
4.10
2.25
0.56
3 days
_ _
—
—
1.49
1.76
1.63
1.47
1.64
—
—
—
—
—
4 days
— —
--
--
1.43
1.69
1.76
1.64
1.65
2.84
0.39
4.01
1.98
0.50
5 days
1.37
0.84
1.17
__
__
__
__
__
__
_ _
-_
—
—
6 days

— _
«~
_ _
__
— —
_ .
__
_ —
__
3.16
1.60
0.58
7 days

__
_ _
•• —
_ _
1.58
_ _
1.28
. —
. _
. _
— —
	
CT>

-------
                                  GLOSSARY


acclimation:  Period during which the nvicroogranisms  become  accustomed to
     their new environment and substrate.

activated sludge process:   A method of secondary wastewater  treatment in which
     a mixture of wastewater and activated  sludge is  agitated  and  aerated.
     The activated sludge  is subsequently separated from the treated waste-
     water (mixed liquor)  by sedimentation, and wasted or returned to the
     process as needed.

aeration:  The bringing about of intimate contact between air  and  liquid by
     one of the following  methods:  spraying the liquid in the air, bubbling
     air through the liquid, or agitation of the liquid to promote surface
     absorption of air.

apparent loading:  The amount of soluble organic material [as  measured  by any
     suitable test such as TOC) removed by a Powdered Activated Carbon  (PAC)
     system, less the amount of removed by a biological system at  the same
     sludge age divided by the applied carbon dosage.

            A™av,0n+ i«a,Hr,« - AOrganlcs CPAC) - AOrganlcs (BIO)
            Apparent Loading -- *  App1i                 -
                                     App1ie      >n  osage

average daily flow:  The average quantity of wastewater passing through  the
     system in a 24 hour period.

biochemical oxygen demand (BOD):  A measure of the oxygen necessary to satisfy
     the requirements for the aerobic decomposition of the decomposable  orga-
     nic matter 1na liquid by bacteria. The standard (BOD5) is five days at
     20° C.

biological fouling:  A decrease 1n the real loading on the Powdered Activated
     Carbon (PAC) as a result of micro-organtsms and their by-products on the
     carbon surface.

biological seed:  Sludge which is mixed with      composed wastewater for the
     purpose of Introducing acclimated organisms, thereby accelerating the
     initial stages of biological growth and the decomposition of organtcs
     in the wastewater.

chemical oxygen demand (COD) :  A measure of the oxygen required to approach
     total oxidation of the organic matter 1n the waste.
                                      97

-------
clarifier:  A tank or basin, in which wastewater is retained for a  sufficient
     time, and in which the velocity of flow is sufficiently low to remove
     by gravity a part of the suspended matter.

coefficient of determination (R2):   Statistical device used to measure the
     "goodness of fit" of the regression analysis defined by the following
     equation:
                                                   /\
                R2 _ regression sum of squares _
                       total sum of squares
     where:
                Y-j = observed y
                ^
                Y-J = y calculated from regression coefficients

                 y = mean value of observed y

completely mixed activate sludge:  Treatment system in which the untreated
     wastewater is instantly mixed through the entire aeration basin.

composite sample:  Integrated sample collected as a portion of the  total  flow.

complex cyanides:  Compounds containing the cyanide group, CN~, in  combination
     with other elements forming a complex radical, such as ferricyanide  and
     ferrocyanide, Fe(CN);3 and Fe(CN)6", respectively.

CuCl2:  Cupric chloride.

cyanides:  Compounds containing the CN" group, in both the simple and/or  comp-
     lex modes,unless otherwise noted.

detention time:  Period of time required for a liquid to flow through a tank
     or unit.

dissolved oxygen (DO):  Free or uncombined oxygen in a liquid.

effluent:  Liquid flowing out of the treatment system.

filtrate:  Liquid passing through a filtering medium with a specified pore
     size.

floe:  Small gelatinous masses, formed in water or wastewater by biochemical
     processes, or by agglomeration.

flocculation:  The bringing together of flocculating particles by hydraulic
     or mechanical means.

influent:  Liquid flowing into a basin or treatment plant.

mean:  Average value of all observations defined x = _j!l

          n = number of observations                  n
         Xj = value of "ith" observation

                                      98

-------
milligrams per liter (mg/A):   The weight of material  in  one  liter of  liquid.

mixed liquor (ML):  A mixture of sludge and wastewater  in  a  biological  reac-
     tion tank undergoing biological  degradation  in  an  activated sludge system.

nutrient:  Any substance absorbed by organisms which promotes  growth  and  re-
     placement of cellular parts, such a phosphorous.

PAC:  Powdered activated carbon.

pH:  The negative logarithim of the hydrogen ion  concentration.   It  is  used
     to express the intensity of the acid or alkaline condition  of a  solution.

settleable solids:  Suspended solids which will settle in  sedimentation basins
     (clarifiers) in normal detention times.

simple cyanides:  Cyanide that may be determined by the Wood  River  modified
     Roberts-Jackson procedure.  This procedure measures the amount  of free
     cyanide as HCN, which includes readily hydrolyzed metal cyanide com-
     plexes such as nickel and copper cyanides.  Cyanides  not included are
     bound in complex form, such as ferricyanide,  and ferrocyanide.

sludge:  The accumulated settled solids separated from wastewater in clari-
     fiers, and containing more or less water to form a semi-liquid  mass.

sludge age:  The average total time of detention of a suspended solids parti-
     cle in a system.  It  is defined as the total weight of suspended solids
     in the aeration basin divided by the total weight of suspended  solids  in
     the effluent and otherwise wasted per day.

sludge volume index (SVI):  The volume of sludge occupied by one gram of
     activated sludge after 30 minutes of quiescent settling in a 1,000 ml
     graduated cylinder.

standard deviation(s):  Measure of the deviation of the x's form the sample
     mean.  The square root of the variance, which measures the dispersion
     of the distribution.
           For paired observations the sample standard deviation is:
                 s  =  /£(xi - x2)  -  (mean difference)'
                          n

           Then  the unbiased standard deviation is:

                                   s
                                 / n-1

 substrate:   Raw waste  feed  on which  a  microorganism  grows  or  is  placed to
      grow by decomposing  the waste material.
                                     99

-------
 substrate removal:  The total BOD in plant influent, minus the soluble BOD in
     plant effluent, divided by the total influent BOD.

 supernatant liquor:  The liquid overlying settled solids.

 suspended solids (SS or TSS):  The quantity of material deposited when a quan-
     tity of wastewater is filtered through an asbestos mat in a Gooch crucible
     or equal method.

 t-test:  To test a hypothesis assuming a normally distributed variable in the
     t-distribution can be used to determine the probability level  of occur-
     rence.  It is defined as follows:
                             T     =  (x-yo)   n
                             Vl  ~  —§	
     where:

                x = mean sample value

               y0 = true mean hypothesized

                n = number of observations

              n-1 = degrees of freedom

                s = standard deviation

total organic carbon (TOC):  The amount of carbon, measured as  C,  in the form
     of organic compounds, dissolved in an aqueous solution.

total solids (TS):   The solids in the wastewater,  both suspended and dissolved.

volatile suspended  solids (VSS):  The quantity of suspended solids in waste-
     water that are lost on ignition of the total  suspended solids.

waste sludge:  Sludge which is no longer needed in the treatment system  and
     therefore is removed and disposed of.
                                     100

-------
                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before completing)
1. REPORT NO.
    EPA-600/2-80-125
                              2.
                                                            3. RECIPIENT'S ACCESSION-NO.
4. TITLE ANDSUBTITLE
  Cyanide Removal From Refinery  Wastewater Using Powderec
  Activated  Carbon
                                                            5. REPORT DATE
                                                            _ May 1980  .
                                                            6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)

  James E  Huff
  Tpffrpy Mr  B-i ,
                                                            8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGARfZATION NAME AND ADDRESS
  IIT Research  Institute
  10 West 35th  Street
  Chicago,  IL  60616
                                                            10. PROGRAM ELEMENT NO.

                                                                  1BB610	
                                                            11. CONTRACT/GRANT NO.
                                                               EPA  R8QAQ2-9-01
12. SPONSORING AGENCY NAME AND ADDRESS
  Robert S. Kerr Environmental Research Laboratory
  Office of Research and Development
  U.S. Environmental Protection Agency
  Ada, Oklahoma  74820
                                                             13. TYPE OF REPORT AND PERIOD COVERED
                                                              Final 9/1975—2/1977	
                                                             14. SPONSORING AGENCY CODE
                                                                  EPA/600/15
15. SUPPLEMENTARY NOTES
16. ABSTRACT
       The  objective of  this  project was  to  evaluate the  removal of low  level
  cyanide in petroleum refinery wastewater by the addition  of powdered activated
  carbon and cupric chloride  to an activated sludge unit.   The activated carbon
  and cupric chloride act  as  catalyst in  the oxidation and  destruction of  the
  cyanides.

       A two-phase study was  carried out  to  develop the process.  The first phase
  consisted of a bench-scale  study using  solutions of metal-cyanide complexes
  in order  to determine  the mechanics of  cyanide destruction.  The second  phase
  consisted of bench-scale tests using actual refinery wastes.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                               b.IDENTIFIERS/OPEN ENDED TERMS
                                                                             COSATl Field/Group
  Activated Carbon Treatment
  Cyanides
  Activated Sludge Process
  Petroleum Refining
                                                 Cupric  Chloride
68D
18. DISTRIBUTION STATEMENT
  Release  to Public
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                                                  Unclassified
                                                                           21. NO. OF PAGES

                                                                                109
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EPA Form 2220-1 (9-73)
                                             101
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-------