Environmental Protection Technology Series
LONG-TERM EFFECTS OF LAND  APPLICATION
                  OF DOMESTIC  WASTEWATER:
                            Hoilister,  California,
                          Rapid Infiltration  Site
                       Robert S. Kerr Environmental Research Laboratory
                               Office of Research and Development
                              U.S. Environmental Protection Agency
                                       Ada, Oklahoma 74820

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                 RESEARCH REPORTING SERIES

 Research reports of the Office of Research and Development, U.S. Environmental
 Protection Agency, have been grouped into nine series. These nine broad cate-
 gories were established to facilitate further development and application of en-
 vironmental technology. Elimination  of traditional grouping  was consciously
 planned to foster technology transfer and a maximum interface in related fields.
 The nine series are:

      1.  Environmental Health Effects Research
      2.  Environmental Protection Technology
      3.  Ecological Research
      4.  Environmental Monitoring
      5.  Socioeconomic Environmental Studies
      6.  Scientific and Technical Assessment Reports (STAR)
      7.  Interagency Energy-Environment Research and Development
      8.  "Special" Reports
      9.  Miscellaneous Reports

 This report has been assigned to the ENVIRONMENTAL PROTECTION TECH-
 NOLOGY series. This series describes research performed to develop and dem-
 onstrate instrumentation, equipment,  and  methodology to repair or prevent en-
 vironmental degradation from point and non-point sources of pollution. This work
 provides the new or improved technology required for the control and treatment
 of pollution sources to meet environmental quality standards.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                                EPA-600/2-78-084
                                                April  1978
           LONG-TERM EFFECTS OF LAND
       APPLICATION OF DOMESTIC WASTEWATER
 Hollister, California, Rapid Infiltration Site
                       by

                Charles E. Pound
                Ronald  VI. Crites
                 James  V. Olson
              Metcalf & Eddy,  Inc.
           Palo  Alto, California  94303
             Contract No.  68-03-2361
                 Project Officer

            William R. Duffer, Ph.D.
          Wastewater Management Branch
Robert S. Kerr Environmental  Research Laboratory
               Ada, Oklahoma 74820
ROBERT S. KERR ENVIRONMENTAL RESEARCH LABORATORY
       OFFICE OF RESEARCH AND DEVELOPMENT
      U.S. ENVIRONMENTAL PROTECTION AGENCY
              ADA, OKLAHOMA  74820

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                                  DISCLAIMER
     This report has been reviewed by the Robert S. Kerr Environmental
Research Laboratory, U.S. Environmental Protection Agency, and approved for
publication.  Approval does not signify that the contents necessarily reflect
the views and policies of the U.S. Environmental Protection Agency,'nor does
mention of trade names or commercial products constitute endorsement or
recommendation for use.
                                     11

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    FOREWORD
     The Environmental Protection Agency was established  to  coordinate
the administration of major Federal  programs designed  to  protect  the
quality of our environment.

     An important part of the agency's effort involves the search for
information about environmental  problems, management techniques,  and new
technologies through which optimum use of the nation's land  and water
resources can be assured and the threat pollution poses to the welfare
of the American people can be minimized.

     EPA's Office of Research and Development conducts this  search
through a nationwide network of research facilities.   As  one of these
facilities, the Robert S. Kerr Environmental Research  Laboratory  is
responsible for the management of programs including the  development and
demonstration of soil and other natural systems for the treatment and
management of municipal wastewaters.

     Although land application of municipal  wastewaters has  been  prac-
ticed for years, there has been a growing and widespread  interest in
this practice in recent years.  The use of land application  received
major impetus with the passage of the 1972 amendments  to  the Federal
Water Pollution Control Act.  The 1977 amendments to the  Act gave
further encouragement to the use of land application and  provided
certain incentives for the funding of these systems through  the con-
struction grants program.  With the widespread implementation of  land
application systems, there is an urgent need for answers  to  several
major questions.  One of these questions regards the long-term effects
of land application on the soil, crops, groundwater, and other environ-
mental components.  This report is one in a series of ten which documents
the effects of long-term wastewater application at selected  irrigation
and rapid infiltration study sites.   These case studies should provide
new insight into the long-term effects of land application of municipal
wastewaters.

     This report contributes to the knowledge which is essential  for  the
EPA to meet the requirements of environmental laws and enforce pollution
control standards which are reasonable, cost effective, and  provide
adequate protection for the American public.

                                      C
                    o***^

William C. Galegar
Director
Robert S. Kerr Environmental Research Laboratory
         iii

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                                    ABSTRACT


      The objective of this  study  was  to  evaluate  the  long-term effects of
 applying municipal  wastewater after primary  treatment to  the land using the
 rapid infiltration technique.   This was  accomplished  by analyzing groundwater
 quality and soil  chemistry  at a site  with  a  long  operating history.

      Primary municipal  effluent has been applied  continuously to rapid
 infiltration basins at  Hollister, California, for more than 30 years.  The
 current daily flow is 43.8  L/s  (1.0 Mgal/d).  Annual  wastewater application
 equals  15.4 m (51  ft) to 20 infiltration basins intermittently flooded for 1
 to  2  days  every 14  to 21 days,  depending on  basin size and season of year.

      Infiltration  rates were  determined, subsurface hydrology was logged, and
 water table response  to wastewater  application was monitored.  A sampling and
 analysis program covering a 1 year  period included samples from (1) primary
 effluent,  (2)  onsite  and control site soil  profiles,  and  (3) groundwater at
 the site and upgradient and downgradient of groundwater movement from the
 site.
™n   nn      of the Pr1mary effl uent and groundwater results indicated that
COD, BOD, TOC, nitrogen, and fecal coliform bacteria were effectively reduced
after percolation through 7 m (22 ft) of unsaturated gravelly and sandy loam
soil.  Effective phosphorus removal required longer travel distances but the
sorption capacity of the soil has not been exceeded after 30 years of
continuous wastewater application.  Trace element retention by the soil  was
low; however, only lead exceeded EPA drinking water limits in the wastewater
and shallow groundwater aquifer.  Iron and manganese are both being leached
from the soil with the percolating wastewater.  Only slight boron removal  in
the percolate was observed.
          report was submitted in fulfillment of Contract No.  68-03-2361  by
wetcalf & Eddy, Inc., under the sponsorship of the U.S.  Environmental
Protection Agency.   This report covers the period January 2,  1976,  to
September 2, 1977,  and work was completed as of December 2,  1977.
                                     iv

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                                  CONTENTS
Foreword [[[   i i 1
Abstract [[[ .    ™
Figures [[[ .........  vil1
Tabl es [[[     *
List of Abbreviations [[[  x111
List of Symbols [[[ xiy
Acknowl edgments [[[   xvi

Section

   1     INTRODUCTION [[[  1
           Background [[[  1
           Objective [[[  2
           Specific Goals ................................................  2

   2     CONCLUSIONS [[[  4
           General [[[  4
           Hydrogeology .......................... ........................  4
           Soil Physical  Properties .............. ........................  4
           Infiltration Rates ............................................  5
           Soi 1 Chemistry .................... ............................  5
             pH and Cal ci urn Carbonate ....................................  5
             Organic Matter ..............................................  5
             Nitrogen [[[  6
             Phosphorus ..................................................  6
             Boron [[[  6

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                           CONTENTS  (Continued)

 6      METHODOLOGY	 22
         Site  Selection	 22
         Study PI an	 25
           Effluent  Sampling	 27
           Groundwater Sampling	 27
           Soil Sampling and Analytical  Procedures	 28

 7      HYDROGEOLOGIC  INVESTIGATION	 30
         Regional Hydrogeology	 30
         Observation  Wells	 33
         Site  Hydrogeology	 37
         Water Level  Response	 41
           Shallow Wells	 41
           Intermediate and Deep Wells	 41
         Summary and  Discussion	 45

8      SOIL INVESTIGATION	 46
         Introduction	 46
         Physical Properties	 47
           Particle Size	 47
           Surface Area	 49
           Bulk Density	 50
           Infiltration Studies	 51
        Soil Chemistry	 55
           Introduction	 55
          Statistical Analysis of Soil  Data	 58
          pH and Calcium Carbonate	 62
          Conductivity of the Saturated Extract	 64
          Organic Matter	 65
          Nitrogen	 66
          Phosphorus	 68
          Boron	 75
          CEC and Exchangeable Cations	 76
          Heavy  Metals	 82
          Agricultural  Potential  of Soils Treated with Heavy Metals... 87

9     GROUNDWATER QUALITY  INVESTIGATION	 90
        Introduction	 90
        Statistical  Analysis of  Groundwater Data	 90
        COD,  BOD,  and TOC	 92
        Residual  Organics  (Carbon  Chloroform Extract)	 94
        Nitrogen	 94
        Phosphorus	 96
        Fecal  Coliform  and  Total Coliform Bacteria	 97
        Dissolved  Solids	TOO
          Total Dissolved Solids and  Electrical Conductivity	100
          Exchangeable  Cations and  Sodium Adsorption Ratio	100
        Major  Anions	101
        Suspended  Solids	102
                                   vi

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                    CONTENTS (Concluded)

  Al kal inity	102
  Boron		103
  Fl uori de	103
  Trace Elements	104

REFERENCES	108

APPENDIX A	116

APPENDIX B	117

APPENDIX C	139
                             vii

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                                    FIGURES

Number                                                                    Page
   1      Location of study site Hollister,  California	   11
   2      Hollister rapid infiltration system facilities	   12
   3      Observation well  locations  at the  Hollister
         rapid infiltration site	   15
   4      Proposed soil  sampling grid locations  at  the
         Hollister rapid infiltration site	   26
   5      Relation of groundwater subbasins  and
         subunits to geologic  features	   31
   6      Hydrogeology of Hollister rapid  infiltration site	   32
   7      Observation well  sites	   34
   8      Typical  observation well construction  and drilling rig	   36
   9      North-south geologic  section	   38
  10      East-west geologic section	   39
  11      Water levels in  intermediate and deep
         observation wells  on  four selected days	   40
  12      Conti nuous  water  1 eve!  recorders	   42
  13      Water level  response  to  basin flooding
         in observation welIs  5A  and  3A	   43
  14      Water level  response  of  intermediate and deep wells	   44
  15      Physico-chemical  properties  of soils
         as related  to textural classification	   47
  16      Cylinder infiltrometers	   53
  17      Vertical  distribution  of soil  pH	   63
  18      Vertical  distribution  of soil  calcium carbonate	   63
  19      Vertical  distribution  of saturated paste, soil conductivity	   65
  20      Vertical  distribution  of soil  organic matter	,	   66
  21      Vertical distribution  of soil  total-nitrogen	   67
  22      Vertical distribution  of soil  organic-nitrogen	...   67
  23      Vertical distribution  of soil  total phosphorus	  69

                                    viii

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                             FIGURES (Concluded)
Number                                                                   Page
  24     Vertical  distribution of soil  bicarbonate
         extractabl e phosphorus	   69
  25     Logarithmic plot of Freundlich regression equation
         to the experimental data for sorbed phosphorus as
         a function of time and equilibrium concentration.
         Control site composite, 0-16 cm depth		   71
  26     Logarithmic plot of Freundlich regression equation
         to the experimental data for sorbed phosphorus as
         a function of time and equilibrium concentration.
         Treatment site composite, 0-16 cm depth	   71
  27     Dynamically predicted values versus equilibrium
         observed values of sorption rates (3S/3t).  Model 1	   74
  28     Dynamically predicted values versus equilibrium
         observed values of sorption rates (as/3t).  Model 2	   74
  29     Dynamically predicted values versus equilibrium
         observed values of log sorption rates (3S/at).  Model 3	  74
  30     Vertical distribution of soil boron	  76
  31     Vertical distribution of cation exchange capacity	  79
  32     Vertical distribution of exchangeable sodium	  79
  33     Vertical distribution of exchangeable potassium	  79
  34     Vertical distribution of exchangeable magnesium	  79
  35     Vertical distribution of exchangeable calcium	  79
  36     Vertical distribution of DTPA-extractable  iron	  83
  37     Vertical distribution of DTPA-extractable  manganese	  83
  38     Vertical distribution of DTPA-extractable  cobalt	  85
  39     Vertical distribution of DTPA-extractable  nickel	  85
  40     Vertical distribution of DTPA-extractable  cadmium	  85
  41     Vertical distribution of DTPA-extractable  zinc	  85
  42     Vertical distribution of DTPA-extractable  copper	  86
  43     Vertical  distribution of DTPA-extractable  lead	  86
                                     ix

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                                   TABLES

Number                                                                   Page
   1       Operating Factors,  Hollister Rapid Infiltration System	   10
   2       Historical  Climatic Data, Hollister,  California	   17
   3       Climatic Data During Study Period, Hollister, California	   18
   4       City of Hollister Water Supply Quality	   19
   5       City of Hollister Effluent Wastewater Quality	   20
   6       Wastewater  Consti tuent  Loadi ng Rates	   21
   7       List of Candidate Rapid Infiltration  Sites
          in  California,  October  1975...	   23
   8       Initial  Site  Screening  Criteria	   24
   9       Summary of  Design and Operating  Information
          on  Six  Candidate  Sites	   24
  10       Summary of  Sampling  Program	   25
  11       Soil  Constituents and Analytical Procedures	   29
  12       Observation Wei 1  Construct! on  Data	   37
  13       Particle  Size Distribution in  Soil Sample From
          the  Hollister Rapid  Infiltration Site	  48
  14       Surface Area Per Unit Soil Mass	  49
                                                o
  15       Estimated Surface Area  Distribution (cm )
          For A 100 g Sampl e of Soi 1	  50
  16       Soil Bulk Density Measurements (g/cm  ) and
          Calculated Soil Porosities	  51
  17       Basin Flooding  Infiltration Rates	  52
  18       Cylinder  Infiltrometer  Infiltration Rates	  54
  19       Results of Soil Chemical Analyses	  56
  20      Analysis of Variance For Selected Soil Parameters	  61
  21      Sample Output For Statistical Analysis of
         Soi 1 Data	  62
 22      Calculated Freundlich Coefficients From
         Phosphorus Sorption  Isotherms	  70

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                             TABLES (Continued)
Number                                                                   Page
  23      Evaluation of Three Dynamic Phosphorus Sorption Models	   73
  24      Measured and Calculated Exchange Ratios For
          Soils at the Hollister Rapid Infiltration Site	   80
  25      Exchangeable Mg/Ca Ratio	   81
  26      Comparison of Soil Microelement Content After
          Long-Term Sludge and Wastewater Application	   88
  27      Comparison of Soil DTPA-Extractable Heavy Metal
          Wi th Heavy Metal Contents of Sel ected Crops	   88
  28      Average Effluent and Groundwater Quality
          Resul ts	   91
  29      Sampl e Output From SPSS Oneway	   93
  30      Comparison of Trace Element Levels to
          Irrigation and Drinking Water Limits	  105
 A-l      Wastewater Quality Results 	  116
 B-l      pH.	  117
 B-2      Calcium Carbonate	  118
 B-3      Electrical Conductivity Soil Extract	  119
 B-4      Organic Matter	  120
 B-5      Total Nitrogen	  121
 B-6      Organic Nitrogen	  122
 B-7      Total Phosphorus	 123
 B-8      Bicarbonate Extractable Phosphorus	 124
 B-9      Boron	 125
 B-10     Cation Exchange Capacity	 126
 B-ll     Exchangeable Sodium	 127
 B-12     Exchangeable Potassium	 128
 B-l3     Exchangeable Magnesium	 129
 B-l4     Exchangeable Calcium	 130
 B-l 5     DTPA-Iron	 131
 B-l 6     DTPA-Manganese	 132
 B-l 7     DTPA-Ni ckel	 133
 B-18     DTPA-Cobalt	 134
 B-l 9     DTPA-Zi nc	 135
 B- 20     DTPA-Cadmi urn	 136
                                     xi

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                             TABLES (Concluded)
Number                                                                    Page
 B-21     DTPA-Copper	  ] 37
 B-22     DTPA-Lead	  138
 C-l      Well  3A Groundwater Quality Results	  139
 C-2      Well  5A Groundwater Quality Results	  140
 C-3      Well  IB Groundwater Quality Results	  141
 C-4      Well  3B Groundwater Quality Results	  142
 C-5      Well  1C Groundwater Quality Results	  143
 C-6      Well  2C Groundwater Quality Results	  144
 C-7      Well  4C Groundwater Quality Results	  145
 C-8      Well  6C Groundwater Quality Results	  146
 C-9      Well  7C Groundwater Quality Results	  147
 C-10     Well  8C  Groundwater Quality Results	  148
 C-ll     Well  9C  Groundwater  Quality Results	  149
                                   xi 1

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                                LIST  OF  ABBREVIATIONS
                                         Special
ABS      Alkylbenzenesulfonate
ANOVA    Analysis of variance
BMDP2V   Computer program designation
         to generate two-way analysis
         of variance
BOD      Biochemical oxygen demand
CCE      Carbon chloriform extract
CEC      Cation exchange capacity
COD      Chemical oxygen demand
DTPA     Diethylenetriamine pentacetic
         acid
EC       Electrical conductivity
E. Coli  Escherichia coli
EPA      Environmental Protection Agency
EPR      Exchangeable potassium ratio
ESR      Exchangeable sodium ratio
JTU      Jackson  turbidity units
LAS      Linear alkyl  benzene  sulfanate
M        Molarity
N        Normality
Nm       Not measurable
PAR      Potassium adsorption  ratio
PVC      Polyvinylchloride
SAR      Sodium adsorption ratio
SPSS     Statistical package for the
         social  sciences
SS       Suspended solids
IDS      Total dissolved solids
TEA      Triethanolamine
TKN      Total Kjeldahl nitrogen
TOC      Total organic carbon
USPHS   United  States Public Health
         Service
                                        Quanti ty
                           kg/ha        Kilogram per  hectare
                           Ib/acre      Pound  per acre
                           mg/kg        Milligram per kilogram
                           meq/100 g    Mi 111 equivalents  per
                                        100 grams
                           meq/L        MilHequlvalents  per  litre
                           yg/g         Micrograms per  gram
                           pmhos/cm     Mlcromhos per centimeter
                           ppm          Parts  per million
                           g/cm         grams  per cubic centimeter
                                            X111.

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                                          LIST OF SYMBOLS
 %p
 3s/3t
 2.5 Y 5/2
 B,m,a,b,d
 A
Ap, Al
Cl, C2
                                           Miscellaneous
 Degrees Celsius
 Soil particle density
 True mean of a subscripted
 soil variable
 Level  of significance attached
 to statistical  test;  the  proba-
 bility of rejecting a true
 hypothesis;  also  a multiple
 regression constant
 Percent
 Percent pore  volume
 Partial  differential  of sorbed
 phosphorus with respect to time
 Soil Conservation Service  (SCS)
 soil color symbol
 Multiple regression constants
 Soil surface area (Eq. 1); also
 shallow well  designation
Soil morphogenic master horizon
designations
Chemical activity  of solution
ions
(Ax), (Bx)  Exchanger concentrations
B           Intermediate well
C           Deep well
C:N         Carbon  to  nitrogen ratio
                                                         D1'D2'D3'D4   Depth Increments  1  through 4
                                                                       Soil  bulk density
                                                                       Oxidation-reduction potential
                                                                       Variance ratio
                                                                       Selectivity coefficient
 ub
 Eh
                                                         k,n
N
P
r
S
t
                                         Mathematical
                                        ±     Plus or minus
                                        >     Greater than
                                        <     Less than
                                        1:1   1 to 1 ratio
 Constants related to energy of
 sorption (Eq. 4); also multiple
 regression constants
 Hypothesis
 Mass of soil
 Probability level  relative  to
 predetermined "a"  value; also
 phosphorus
 Soil  particle radius
 Correlation coefficient squared
 Sorbed  phosphorus
 Statistic  for testing difference
 between means
 Control site statistical treat-
 ments
Application site statistical
 treatment
Observation
                                                xiv

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          LIST  OF  SYMBOLS  (Continued)
Elements
Ag
As
B
Ba
Ca
Cl
Cd
Co
Cr
Cu
F
Fe
H

Silver
Arsenic
Boron
Barium
Calcium
Chlorine
Cadmium
Cobalt
Chromium
Copper
Fluorine
Iron
Hydrogen

Hg
K
Mg
Mn
N
Na
Ni
P
Pb
Se
Zn



Mercury
Potassium
Magnesium
Manganese
Nitrogen
Sodium
Nickel
Phosphorus
Lead
Selenium
Zinc



CaC03
CaF2
CaN03
Ca5(P04)3F
H20
HCL
H3B03
HCL04
K2Cr207
MgHC03
Mn02
NaC03
NaHC03
NH3-N
                                      NH4OAc
                                      N02-N
                                      N03-N
                                      PO,
                                      so,
 -3
I
 -2
      Compounds
Calcium carbonate
Calcium fluoride
Calcium nitrate
Fluorapatite
Water
Hydrochloric  acid
Boric acid
Perchloric acid
Potassium dichromate
Magnesium bicarbonate
Manganese dioxide
Sodium carbonate
Sodium bicarbonate
Ammonia-Nitrogen
Ammonium ion
Ammonium acetate
Nitrogen gas
Nitrous oxide
Nitrite nitrogen
Nitrate nitrogen
Oxygen gas
Phosphate
Sulfate
                                XV

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                               ACKNOWLEDGMENTS
     The  authors wish  to  thank the many individuals and agencies whose
 assistance was  essential  to the  success of this project.  The support of Mr.
 Roger Grimsley, City Engineer, and Mr. Ray Petregon, Treatment Plant
 Operator, City  of Hoi lister, is  gratefully acknowledged.

     Interpretation and statistical analysis of soil chemistry results were
 performed by Mr. Paul  E.  Levine, Soil Scientist, Metcalf & Eddy, Inc., and
 Professor Richard Burau,  Department of Land, Air and Water Resources, Soil
 Section, University of California, Davis.  Supervision of observation well
 construction and interpretation  of hydrogeologic data were performed by the
 Metcalf & Eddy, Inc., Geotechnical Department in Boston, Massachusetts.
 Laboratory analysis of effluent, groundwater, and soil samples was performed
 by the Metcalf  & Eddy, Inc., Water Chemistry Laboratory in Palo Alto,
 California, under the direction  of Dr. Charles D.  Siebenthal, Director of
 Laboratory Services, and Mr. Paul E.  Levine (soil  testing).   Richard D.
 Shedden, Environmental Engineer, aided in the infiltration studies and
 sampling program.

     The authors wish to express appreciation to the Robert  S.  Kerr
 Environmental Research Laboratory for its support  of this project,  and
 especially to Dr.  William R. Duffer,  Project Officer,  for his guidance
 throughout the course of the study.

     Special  thanks  are also due to Mrs.  June B. Miller for  her very capable
job of editing this  report.
                                    xvi

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                                  SECTION  1

                                 INTRODUCTION
BACKGROUND

     Land treatment processes for renovating wastewater  have  been
demonstrated successfully on many occasions in the past,  and  in  the  last 40
years the number of land treatment facilities has increased steadily in the
United States [1, 2].  Land treatment has demonstrated equal  or  superior
pollutant removal when compared to conventional  treatment and, in many cases,
beneficial water reuse is an integral  part of the process.  In the  future, as
construction costs increase and water resources become more scarce,  land
treatment will become even more competitive as a wastewater management
alternative.

     Land treatment of municipal wastewater encompasses  a wide variety of
processes or methods.  The three principal processes, are:

     1.   SIow rate

     2.   Rapid  infiltration

     3.   Overland flow

     The major concepts  involved in these processes are defined  herein.

     The  term slow rate  land treatment is used to focus attention on
wastewater  treatment  rather than on irrigation of crops.  However, in slow
rate systems, vegetation is a critical component  for managing water and
nutrients.  The  applied  wastewater is treated as  it flows through the soil
matrix, and a portion of the flow percolates  to the groundwater.  Surface
runoff of the applied water is  generally  not  allowed.

     In rapid infiltration land treatment (referred to in previous U.S.
Environmental Protection Agency (EPA) reports as  infiltration-percolation),
most of the applied wastewater  percolates through the soil, and the treated
effluent  eventually reaches the groundwater.  The wastewater is applied to
rapidly permeable  soils, such as sands and  loamy  sands, by spreading in
basins or by  sprinkling, and is treated as  it travels through the soil
matrix.   Vegetation  is not usually used,  but  there are some exceptions.   In
many cases, recovery  of  renovated water is  an integral part of the  system.
This can  be accomplished using  underdrains  or wells.

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     In overland flow land treatment, wastewater is applied over the upper
reaches of sloped terraces and allowed to flow across the vegetated surface
to runoff collection ditches.  The wastewater is renovated by physical,
chemical, and biological means as it flows in a thin film down the relatively
impermeable slope.

     With the relatively large number of successful land treatment systems
currently in operation, a wealth of monitoring data should be available.
Most of the existing land treatment systems, however, are inadequately
monitored.  As a result, there is concern about limitations on the operating
life of land treatment systems.  Specific questions regarding (1)  the level
of preappl ication treatment necessary;  (2) the movement of persistent
organics, nitrates, and trace elements  into groundwaters; (3) accumulations
of salt or toxic materials in soils; or (4) translocation of potentially
toxic trace elements from the soil  into crops remain to be answered.  Answers
to these and other related questions will assure that sound design criteria
can be established and that land treatment systems can be implemented without
risk to public health.

     The EPA and others have recently funded research on the environmental
effects of different land application techniques in pilot and experimental
studies; however, operating land treatment systems with substantial  longevity
have not been studied or monitored  extensively.   Complete case studies of  the
effects of groundwater quality and  movement in a number of these systems
should provide some answers to the  concerns that were mentioned.   This report
presents the findings of a research study at a 3U-year old land treatment
system at Hollister, California,  where  primary municipal  effluent  is  applied
to rapid infiltration basins.

OBJECTIVE

     The objective of this study was to evaluate the long-term effects on
groundwater quality and soil chemistry at a site with a long operating
history of applying primary municipal effluent to the land using the rapid
infiltration technique.  The long-term effects were determined by comparing
groundwater quality and soil chemistry  data at this site with data from  a
nearby control site having similar physical characteristics and management
except that wastewater has never been applied.

SPECIFIC GOALS

     To accomplish the overall objective, the following specific goals were
selected:

     1.   Determine the nature of the site hydrogeology for the purpose  of
          relating subsurface conditions to the efficiency of the  soil matrix
          in removing constituents  from the applied primary effluent and,
          subsequently, the danger  of contaminating native groundwater
          aquifers.

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2.   Determine the current treatment efficiency of the soil  matrix by
     comparing the quality of the applied primary effluent with the
     groundwater at the site.

3.   Determine the long-term ability of the soil  matrix to treat
     wastewater by calculating the mass of selected constituents
     retained in the soil  profile applied over the 30 year operating
     life.

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                                   SECTION  2

                                  CONCLUSIONS
 GENERAL

      Effective  long-term  treatment capability has been demonstrated at a
 rapid infiltration  site where  unchlorinated primary effluent from the City of
 Hoi lister, California, has been managed  successfully for 30 years.  Thus, a
 fully managed groundwater recharge system for renovating primary municipal
 effluent  by  surface spreading  at high rates should provide excellent
 treatment for a long period of time.

 HYDROGEOLOGY

 1.    The  subsurface hydrology  at the rapid infiltration site consists of a
      complex network of water-bearing and impermeable material deposited as
      stream  sediments.  Beneath the site there are probably three water-
      bearing zones—shallow, less than 12.7 m (42 ft); intermediate, between
      15 m to 25 m (45 to  75 ft); and deep, greater than 55 m (165 ft)--each
      separated by semi continuous to continuous clay and silt layers.

 2.    The  infiltrating effluent apparently forms a mound caused by perching of
      the  effluent on top  of the clay layer that underlies the shallow
      permeable zone.  When infiltration ceases, the mound subsides mainly by
      lateral outflow from the  site.

 3.    The  intermediate wells showed no response to effluent application to the
      infiltration basins.  However, some vertical leakage may be occurring,
      especially where the clay and silt between the shallow and intermediate
      zones is the thinnest.  This conclusion is based on a comparison of
      concentrations of some mobile ions in the shallow and intermediate
      groundwater.

4.    The deep observation wells did not show any response to effluent
      application.  The lower permeable zone is overlain by an average of 26 m
      (85 ft) of clay and silt that is probably continuous beneath the
      treatment site and is an effective barrier to vertical  groundwater
     movement.

SOIL PHYSICAL PROPERTIES

1.   The most appropriate soil  mapping terms for the alluvial  deposits at the
     site appear to be gravelly sand  or gravelly sandy  loam.

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2.   There were insufficient bulk density measurements  to  determine  if
     conclusive differences existed between control  ana treatment  sites or
     among depth increments.  Measurements taken ranged from  1.27  to 1.72
     g/ciTH.  Lower than average bulk densities of the near surface
     measurements at the treatment sites were probably  the result  of disking
     operations.

INFILTRATION RATES

1.   The maximum long-term infiltration rate obtainable, based on  wastewater
     intake rates monitored over the study period, was  64.6 m/yr  (212  ft/yr).

2.   Initial wastewater intake was slowed by clogging of soil  pores  at the
     surface from wastewater solids applied during the  flooding cycle.
     However, the initial  water intake rate was restored by surface  disking
     between each wastewater application.  No significant  decrease in  soil
     profile infiltration capacity was indicated after 30  years of wastewater
     application.  This conclusion is based on the results of cylinder
     infiltrometer tests at an actual treatment site location and  a  similar
     offsite location where wastewater has never been applied.

3.   The water intake capacity of the treatment site soils did not,  on the
     average, limit the amount of wastewater that could be applied as  measured
     by the time it took for complete infiltration of the  applied  effluent.
     However,  reflcoding a basin without prior surface disking caused a
     greater degree of soil pore clogging and a subsequent lower  infiltration
     rate.

4.   Final infiltration rates measured by cylinder infiltrometers  were
     generally consistent, but overestimated by a factor of 16, the  rates
     obtained by flooding an entire basin.  No significant difference  between
     buffered and unbuffered cylinder infiltrometer infiltration  rates was
     observed.  The clogging effect of wastewater solids was  not  observed
     during cylinder infiltrometer tests comparing primary effluent (SS = 275
     mg/L) and clear tap water.

SOIL CHEMISTRY

pH and Calcium Carbonate

     The addition of wastewater to the treatment site significantly decreased
the soil pH at all depth increments through 3UO cm (10 ft).  Simultaneous
calcium carbonate depletion from the site reduced the buffering capacity of
the soil permitting a pH reduction.  Surface soil pH was less than applied
wastewater pH, suggesting nitrification as an additional mechanism for pH
reduction.

Organic Matter

     Only a slight buildup of organic matter was observed as  a result  of
wastewater application, thus suggesting that the soil microbial population
existed in sufficient numbers to bio-oxidize most of the incoming  organic
matter.

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 Nitrogen

      Significant concentration increases resulting from wastewater treatment
 were observed for the nitrogen species measured (total  and organic nitrogen)
 to a depth of 100 cm (39 in.).  The greatest accumulation of nitrogen was
 found at the surface.  Only 2% of the total  nitrogen applied over  30 years
 was accounted for in the soil  profile.  This suggests the conversion of
 nitrogen to mobile forms and movement from the  soil  profile either to the
 atmosphere or the groundwater.  Groundwater quality  results indicate that 93%
 of the total  nitrogen in the applied wastewater was  removed from solution.

 Phosphorus

      The fact that extractable phosphorus  was so much higher at all sampling
 depths in the treatment site suggests that availability  for plant  uptake was
 high and that soil  solution phosphorus may have passed through the  soil
 profile.   Soil mass balance calculations further suggest significant
 transport of  phosphorus to  the underlying  groundwater.

      Laboratory  phosphorus  isotherm  studies  revealed  that  after 30 years of
 wastewater application,  the soil profile at  0 to 16 cm (0  to 6 in.) is
 retaining  68% of  its  experimentally  determined  sorption  capacity.   Existing
 phosphorus sorption models  were  shown  to underestimate the  actual  30 year
 phosphorus sorption capacity by  factors of 4 to  15 based on measured
 phosphorus in Hoi lister treatment site soils.

 Boron

     Boron was observed  to  increase  fourfold in treatment site soils with the
 most extensive accumulation near the surface.

 CEC and Exchangeable Cations

 1.   Wastewater application caused a significant increase in CEC with respect
     to depth and treatment in spite of the pH decrease which simultaneously
     occurred.  It  is therefore presumed that the resulting loss of any  pH
     dependent charge was more than offset by the influx of organic matter
     and mineral  clays in the wastewater.

2.   The data indicate that more exchangeable sodium is present than can  be
     predicted by the calculated ESR (exchangeable  sodium ratio).   One
     explanation  is concentration of sodium on the  surface by evaporation
     before transport through the soil profile.   An alternative explanation
     is that magnesium and calcium are ion-paired,  complexed, or chelated to
     a much greater extent than sodium.  The result is a  low SAR (sodium
     adsorption ratio) value and therefore  the calculated ESR underestimates
     the exchange phase sodium.

 3.   High  ESR values  throughout  the  treatment site profile have not caused
     any apparent decrease  in  infiltration capacity.

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4.   The data indicate that magnesium was selectively replacing calcium in
     the surface 100 cm (39 in.) of the soil.  The newly mobilized calcium
     traveled downward and then appeared to exchange with magnesium at 3UU cm
     (10 ft).

Heavy Metals

1.   The increase in extractable iron throughout the soil profile may result
     from its accumulation by wastewater addition, or from its increased
     extractability as a result of alternating periods of wetness and
     dryness.  There is no doubt, however, that iron was moving through the
     soil profile.

2.   In contrast to iron, manganese was leached by the wastewater from the
     surface 30 cm (1 ft) with some redeposition at depth.

3.   Nickel and cobalt did not accumulate at the surface 30 cm (1 ft) where
     manganese was lost.  However, extractable cobalt was six times higher in
     treatment sites at 300 cm (10 ft) than in control sites at the same
     depth.  Similarly, extractable nickel values doubled at the 100 cm (39
     in.) depth.

4.   Wastewater application caused extractable cadmium to increase
     significantly at 30 cm (1 ft).

5.   The increase in extractable zinc to the 300 cm (10 ft) depth suggests
     that some wastewater applied zinc has passed down to and perhaps through
     this depth.  The apparently higher mobility of zinc, as compared to
     chemically similar cadmium, may be related to its higher input
     concentration in the wastewater.

6.   Significant extractable copper showed a pronounced accumulation in the
     soil profile at 0 to 16 cm (0 to 6 in.).

7.   The levels of soil DTPA-extractable metals will  not adversely affect the
     future agricultural potential  of the Hollister rapid infiltration site.

GROUNDWATER QUALITY

1.   On the basis of existing quality criteria for groundwater, there was no
     evidence that toxic trace elements or potentially pathogenic bacteria
     associated with municipal wastewater were entering the regional
     groundwater supply, at harmful levels, as a result of wastewater
     application to the land at Hollister, California.

2.   Analysis of the wastewater and groundwater results indicates that the
     soil filtration process has effectively reduced the COD, BOD, TOC, and
     fecal  coliform bacteria.   After 30 years of continuous wastewater
     application, COD,  BOD, TOC, and fecal coliforms are reduced by 93, 96,
     96, and 99%, respectively, after percolation through 7 m (22 ft) of
     unsaturated gravelly and sandy loam soil.

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3.   Almost complete nitrification and denitrification of the renovated
     wastewater was indicated by the results after percolation to the shallow
     groundwater.  The favorable carbon to nitrogen ratio of 6:1 apparently
     supplies sufficient energy to sustain denitrifying bacteria.  No
     significant nitrogen increase from land applied wastewater was observed
     in the intermediate or deep observation wells.

4.   Effective phosphorus removal required longer travel  distances.  Shallow
     groundwater phosphorus concentrations indicated that 22 to 35% of the
     wastewater phosphorus is currently being removed after 3U years of
     wastewater application.   This agreed closely with the accumulated mass
     of phosphorus found in the treatment site and soil profile over the 3U
     year operating history.

b.   Trace element concentrations in the shallow groundwater were generally
     the same as the applied effluent indicating little removal.  However,
     levels of all toxic trace elements, except lead, were below EPA drinking
     water standards in the wastewater and shallow groundwater.  Wastewater
     lead concentrations were greater than drinking water limits, but were
     less than concentrations in shallow observation wells.  The indication
     is that lead is being leached from the soil  with the percolating
     wastewater.

6.   Manganese concentrations in the shallow groundwater  were greater than
     wastewater manganese concentrations.   Manganese is being leached from
     the soil  with the percolating wastewater.

7.   Comparison of wastewater and shallow  groundwater iron concentrations
     strongly  suggested that applied iron  is highly mobile and moving through
     the soil  profile.

8.   Only slight boron removal  (14%)  was observed after percolation to the
     shallow groundwater.

9.   Fluoride  concentrations increased slightly in the shallow groundwater
     over the  applied  effluent.

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                                  SECTION 3

                               RECOMMENDATIONS


1.   The operating history,  system operation,  grounawater hydrology,  and
     pollutant removals determined in this study can  be used in  part  to
     design a rapid infiltration system that will  not impair land  resources
     or groundwater resources.

2.   Primary treatment should be considered adequate  and preferred to
     secondary level  pretreatment for control  of nitrogen.   The  carbon to
     nitrogen ratio of primary  effluent favored complete denitrification and
     did not inhibit COD and BOD removal  after relatively short  underground
     travel distance.

3.   A study should be undertaken to determine the soil  depth that is
     necessary to achieve satisfactory wastewater treatment when primary
     effluent is applied at moderate rates.

4.   Monitoring of the Hoi lister rapid infiltration system should  be  expanded
     to further define the  preferred pathway of subsurface flow  from  the
     site.  This would provide  the means to determine the underground travel
     distance necessary to  achieve a higher degree of treatment  than
     determined herein.  Special emphasis should be given to phosphorus and
     fecal coliform removals.

5.   The greatest void of information remaining with  respect to  land
     treatment systems is that  of persistent or refractory organic compounds.
     Uncertainties regarding health effects from transport of these materials
     through the soil from  land applied wastewater must be answered before
     essential design criteria  can be established.  Quantification of basic
     scientific data on organic substances of  known or suspected toxicity  and
     determination of safe  underground travel  distances are major  areas where
     research is needed.

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                                 -   SECTION 4

                               PROJECT DESCRIPTION


      Hoi lister is located  in  the San Juan Valley  35  km (22 miles) inland from
Monterey Bay, 144 km (90 miles)  south of San Francisco (Figure 1).
Currently,  the population  served by sewers in and around Hoi lister is about
10,000.   The rapid infiltration  site is about 1.6 km (1  mile) west of the
city,  150 m (500 ft) south of the San Benito River bed.   A summary of
important operating information  for the Hoi lister wastewater  management
system is shown in Table 1.

                         Table 1.  OPERATING FACTORS,
                      HOLLISTER RAPID INFILTRATION SYSTEM
                 Preapplication treatment            Primary
                 Groundwater level3, m              5.8-9.2
                 Infiltration area, ha              8.8
                 Total basins                      20
                 Annual wastewater application, m/yr   15.4
                 Average daily flow, L/s             43.8
                 Length of operation, yr             30
                 Industrial influence               Yes

                 a.  Shallow water table created by infiltrating effluent.
                 b.  Slaughterhouse * 0.9 L/s and paper recycler = 11.0 L/s,
                    about 27% of total flow.

                 m x 3.281 = ft
                 ha x 2.471 = acre
                 L/s x 0.0228 = Hgal/d


     The  facilities at the Hoi lister rapid infiltration  site  are shown in
Figure 2.   Preapplication treatment consists of primary  clarification of the
untreated influent wastewater.   Sludge from the clarifier is  regularly drawn
off and stored in  a converted Imhoff tank before being applied to sludge
drying beds independent of the  rapid infiltration area.

     A portion of  influent wastewater flow is equalized  in an excavated
earthen reservoir  before entering  the head works and clarifier.   Wastewater
is pumped from the equalizing reservoir each day when the flowrate is lowest
at 2 a.m.
                                      10

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                                                   o
                                                   \
                                                   (
  STOCKTON
SAN FRANCISCO
                                       SANTA CLARA  COUNTY
                         SANTA CRUZ COUNTY




                                  SANTA CRUZ */
    STUDY  SITE

    LOCATION
 PACIFIC
         OCEAN
                                              SALINAS




                                            IONTEREY COUNTY
  HOLLISTER


SAN BEN I TO COUNTY   \
         Figure 1.   Location of study site Hollister, California.
                                   11

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Figure 2.  Facilities plan of Hollister rapid  infiltration system.




                               12

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      The clarified effluent flows by gravity to the effluent treatment area,
which consists of 20 individually controlled infiltration basins.  Basins are
controlled by a combination of alfalfa valves and manually prepared ditches.
One additional basin is kept in reserve for overflow effluent caused by
sporadic hydraulic overloading in the gravity distribution system.

      The total basin area is 8.8 ha (21.7 acres) and the individual basins
range from 0.3 to 0.7 ha (0.6 to 1.8 acres).  Normally, wastewater is applied
to a depth of 30 cm (12 in.), whereas time of application is controlled by
the area of the basin.  Two basins are flooded simultaneously if the daily
flow exceeds the capacity of any individual basin.  This situation occurs in
the smaller basins (<0.37 ha, <0.9 acres) during the wet winter months.

      The interval between wastewater applications ranged from 14 to 21 days
during the study period.  The length of time a basin was flooded and the
interval between applications decreased in the cooler and wet winter months
by 25 to 30%.

      The initial effluent intake rate was much higher than the final intake
rate, because clogging of the soil surface by effluent solids reduced the
rate at which water entered the soil.  The effluent was completely contained
within the basin it was applied to, leaving the site only as subsurface flow.
The average effluent intake rate was 10 cm/d (4 in./d) when basins are
flooded to a depth of 30 cm (12 in.).

      Geologically, the site is located on alluvial deposits of the San Benito
River.  The surface soil from which the basins are constructed is
characterized as Metz sandy loam [3].  According to the Soil Conservation
Service (SCS), soil permeability for Metz sandy loam ranges from 6.4 to 12.8
cm/h (2.5 to 5.0 in./h) in the upper 3 m (10 ft), and 12.8 to 25.4 cm/h (5.0
to 10.0 in./h) through the next 7.5 m (25 ft) [3].

      The water table of the regional groundwater aquifer occurs at 20 m (65
ft) at the site.   Eight observation wells were constructed to monitor
groundwater quality at the Hoi lister rapid infiltration site.  The depth of
each well  from the local ground surface is as follows:
                               Well   Depth,  m  (ft)

                                1A     6      (20)
                                IB    24      (80)
                                1C    48     (160)
                                2A    54     (180)
                                3A     7.6    (25)
                                3B    21      (70)
                                4B    18.3    (61)
                                5A    10.5    (35)
                                     13

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          The wells are 5 cm (2 in.) in diameter constructed from PVC  pipe
with a 0.6 m (2 ft) length of PVC well  screen at the installed depth.
Existing offsite production wells were also monitored.   Well 4C (City  of
Hoi lister) serves as an upgradient control  well  with perforations beginning
at 56 m (185 ft).  Four production wells (6C, 7C, 8C,  and 9C) serve  as
control wells downgradient of groundwater flow from the site.  Observation
well  locations are shown in Figure 3.
                                    14

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  EXISTING
OFFSITE WELLS
  8C
         LEGEND

      A - SHALLOW WELLS
          3-1D m (10-35 ft)

      B - INTERMEDIATE WELLS
          20-24 m (65-80  ft)

      C - DEEP WELLS
          > 48 m (> 160 ft)
                                                                                          RAPID
                                                                                          INFILTRATION
                                                                                          BASINS
                                                                                      METRES
            Figure 3.   Observation well  locations at  the  Hollister rapid  infiltration site.

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                                  SECTION 5

                     RAPID INFILTRATION SYSTEM OPERATION
SYSTEM HISTORY
     Wastewater was first applied to the site in 1922, when an outfall  sewer
was constructed to convey the untreated wastewater away from the city.   At
that time the site was in pasture, and the wastewater was allowed to flood
the general area in a more or less uncontrolled manner.  During the 1920s,
1930s, and 1940s, 10 acres of walnuts were irrigated with a portion of  the
wastewater until the orchards were abandoned.  The wastewater could be
diverted from the pasture area easily and was used mainly because it was more
convenient than pumping from the native groundwater.  Wastewater irrigation
of nursery stock was practiced successfully at the site from 1935 to 1946.
The practice was abandoned when the new infiltration basins were constructed.

     In 1927, an Imhoff tank was constructed to remove settleable solids from
the wastewater before it was applied to the land.   In about 1946, Basins 1
through 13 (north basins) covering 4.9 ha (12.2 acres) were constructed as
shown previously in Figure 2.  Intermittent application of wastewater to the
infiltration basins was initiated at this time.  No recovery or reuse of the
percolated effluent has ever been attempted.

     In 1962, as a result of increased wastewater flow, a gravity clarifier
was constructed to increase solids removal  before applying the effluent to
the infiltration basins.  At that time, the original Imhoff tank was
converted to an unheated sludge digester by removing the interior partitions.
Seven additional basins (14 through 20) covering 4.0 ha (9.9 acres) were
constructed in the late 1960s when hydraulic loading began to exceed the
capacity of the original basins (see Figure 2).

     In 1973, an equalizing reservoir was excavated to accept peak wastewater
flows before entering the clarifier.  Even  with the equalization reservoir,
however, hydraulic capacity of the clarifier was exceeded, resulting in high
concentrations of unsettled solids being applied to the basins.  Construction
of the reservoir removed about 0.4 ha (1.0  acre) of infiltration basins from
service; however, Basins 14 through 20 were simultaneously expanded. The
total  infiltration basin area including an  overflow area is currently 9.1  ha
(22.6 acres).  The net area used for infiltration  is currently 8.8 ha (21.7
acres).

     A slaughterhouse and a corrugated paper recycler were the only
significant industrial  waste sources.   The  slaughterhouse waste had a high
BOD while the recycle operation added a significant solids load.  The total

                                     16

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flow from both sources of 0.9 L/s (0.02 Mgal/d)  and  11.0 L/s  (0.25 Mgal/d),
respectively, amounted to about 27% of the total  flow.

CLIMATE

     The climate in the Hollister area is marked by  warm dry  summers and cool
rainy winters, which is characteristic of the  Central  Coastal  Region of
California.  Although Hollister is separated from the  Pacific Ocean  by
coastal mountains, temperatures are moderated  by the ocean influence.   The
wet season is normally mid-November through mid-April.  On the average,
little or no precipitation occurs from May through August. July is  the month
with the highest average maximum temperature,  while  peak precipitation
usually occurs in January.  The annual evaporation exceeds precipitation in
the Hollister area by several times;  but  the exact ratio will vary  depending
on annual temperature, precipitation, and wind.   Mean  temperatures,
precipitation, and evaporation are compared to those for the  1962-1963
reporting period (only complete data  available)  in Table 2 [4].   The mean
annual "net evaporation" is approximately 150  cm (59 in.)  according  to one
report [5].  The net evaporation for  1962-1963 was 98  crn (39  in.) in which
below average temperatures but above  average precipitation occurred.  The
mean annual net evaporation is approximately 6% of the current annual
wastewater applied to the infiltration basins.

          TABLE 2.  HISTORICAL CLIMATIC DATA,  HOLLISTER, CALIFORNIA
Month
Jul
Aug
Sep
Oct
Nov
Dec
Jan
Feb
Mar
Apr
May
Jun
Annual

Mean
Avg temperature,
ar-tt
Temperature, Precipitation, Evaporatic
°C [6]b cm [6] cm [5]
20.0
19.5
20.0
17.0
13.0
10.0
9.5
11.0
13.0
14.5
16.5
18.5
15.0
0.03
0.05
0.46
1.24
3.30
6.78
6.53
6.30
4.75
2.87
0.89
0.20
33.40
27.56
25.98
20.57
14.96
8.38
3.81
2.36
4.98
9.45
16.00
23.62
25.91
183.59

in ,
Maximum
25.5
28.5
25.5
24.5
21.0
18.0
15.5
20.0
18.5
18.0
20.5
23.5
21.5

Minimum
8.0
9.0
8.0
6.0
3.5
1.0
-1.5
8.5
3.5
5.5
9.0
9.0
5.5
1962-1963 water year [4]b
recipitation, Evaporation,
cm cm
0
Trace
Trace
1.83
0.64
4.52
9.88
7.39
5.36
6.91
0.99
0.25
37.77
22.96
20.68
13.00
9.07
8.00
5.74
4.39
6.27
8.48
7.65
13.06
16.71
136.02
 a. Temperatures reported to nearest 0.5°C.
 b. Only available evaporation data for Hollister.

 cm x 0.3937 - in.
 (°C x 1.8) + 32 = °F
                                      17

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      Available  climatic data for Hollister during the  study  period  is  shown
 in Table 3.   The  weather pattern encountered was highly unusual with respect
 to precipitation.   Although the total annual rainfall  was almost  normal,  the
 monthly distribution  was very unusual.  The largest monthly  rainfall occurred
 in June 1976  which  is normally precipitation free.  Drought  conditions
 prevailed at  Hollister and throughout northern California during  this time
 period.  Based  on available historical climatic data,  it is  estimated that
 the net evaporation during the study period was slightly greater  than normal.

                  TABLE  3.   CLIMATIC DATA DURING STUDY  PERIOD,
                            HOLLISTER, CALIFORNIA [7]
         Month
Avg temperature, °Ca
	 Hours,  Hours,  Hours,  Precipitation,  Relative
Maximum     Minimum >38°C   <7°C   <0°C       cm       humidity,*
May 1976
Jun
Jul
Aug
Sep
Oct
Nov
Dec
Jan 1977
Feb
Mar .
Apr
May
Jun
Jul
Aug
24.0
31.0
27.0
27.0
26.0
25.0
20.0
17.5
14.5
19.5
18.0
24.0
21.0
27.0
30.5
28.5
6.5 1
9.5 14
11.0
12.0
11.0 1
8.5
5.1
-1.0
1.0
3.0
1.0
4.5
7.5
11.0
10.5
13.0 2
56
28
_.
—
—
43
168 22
414 83
418 61
250 9
279 21
195
59
..
—
—
0.0
6.45
0.0
3.07
7.72
1.27
2.29
3.15
2.13
1.14
2.21
0.79
2.21
0.43
0.0
0.0
37.5
32.6
41.4
41.9
41.0
40.8
44.0
31.5
47.2
34.9
28.5
26.9
35.9
38.0
30.5
37.2
        a.  Values reported to the nearest 0.5°C.
        cm x 0.3937 = in.
        (CC x 1.8} + 32 = °F


SURFACE WATER  HYDROLOGY

     The San Benito River  is  the only natural surface water  source  that
potentially has an  effect  on  the Hollister rapid infiltration  site.   The  San
Benito River is generally  dry,  except during unusually heavy rainfall
occurring below the point  of  the Hernandez Dam located some 80 km (50+ miles)
upstream of the study  site.   Regular releases from the Hernandez Reservoir
provide managed recharge of  subsurface aquifers as well as flood control.
Surface flow does not  generally occur as a result of water release  from the
reservoir in the vicinity  of  the study site.  However, groundwater  levels of
                                       18

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the main Hoi lister-San Juan  groundwater basin in the site vicinity can be
affected by the releases  from  Hernandez Reservoir.

WATER AND WASTEWATER CHARACTERISTICS

     The mineral content  of  the  wastewater can be traced directly to the
quality of the domestic water  supply.   Potable water is derived solely from
wells and the quality varies from  source to source.  In general, the water
supply is relatively hard and  high in  total  dissolved solids, sodium,
chloride, sulfate, and boron.  A summary of available water supply quality
data for the City of Hoi lister is  shown in Table 4.  The effluent wastewater
quality results, in which three  24-hour composite samples were taken each
quarter for 1 year, are shown  in Table 5.   Data for each primary effluent
composite sample taken are shown in Appendix A.

              TABLE 4.  CITY OF  HOLLISTER  WATER SUPPLY QUALITY3
                          mg/L  Unless Otherwise Noted
Item
Flow, L/s
General mineral analysis
Hardness (CaO>3)
Bicarbonate (CaCOj)
Carbonate (CaCOj)
Hydroxide
Alkalinity (CaC03)
Ca
Mg
Fe
Hn
Na
Cl
so4
F
N02-N and N03-N
pH, units
Conductivity, pmhos/cm
TDS
Trace elements
As
B
Cu
Pb
Se
Zn
General physical analysis
Color, units
Odor, threshold No.
Turbidity, JTU
Well No. 2
61.3

371
324
0
0
324
63
52
0.0
0.0
132
103
248
0.7
3
7.6
1380
870

0.00
0.6
<0.1
0.02
0.00
0.02

5
1
0.5
Well No. 4
100.7

374
328
0
0
328
61
54
0.0
0.0
130
101
264
0.7
2
7.7
1380
870

0.00
0.7
<0.1
0.02
0.00
0.02

5
1
0.3
Well No. 5
70.1

388
360
0
0
360
68
53
0.2
0.0
130
104
260
0.7
3
7.6
1490
940

0.00
0.6
<0.1
0.02
0.00
0.02

7
1
0.5
Cienega well
21.9

81
	
—
	
	
	
__
__
..
16
20
16
__
1.8
__
250
157

..
0.05

..
__
„

— _
„_
--
               a.  From City of Hollister Water Supply Monitoring data 1973.
               L/s x 0.0228 = Mgal/d
                                     19

-------
TABLE 5.  CITY OF HOLLISTER EFFLUENT WASTEWATER QUALITY
              mg/L Unless Otherwise Noted
Constituent
COD
BOD
TOC
Total N
NH3-N
N-organic
N03-N
Total P
PO/i-P
Total coliforms,
count/100 ml
Fecal conforms,
count/100 ml
pH, units
TDS
Conductivity,
umhos/cm
SS
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
N1
Pb
Se
Zn
SAR
Range a
546-1029
134-414
240-264
29.7-58.5
19.7-44.0
6.7-21.8
0.16-0.8
10.0-21.5
9.0-13.2
4.6x106
92x106-
3.5x106
24x106
7.0-8.1
1016-1593

1230-2480
206-438
416-465
0.95-1.8
0.33-1.08
196-391
31-71
27-76
10.9-16.0
185-490
161-250
<0. 005-0. 01 2
—
<0.1-0.24
<0. 001 -0.008
<0. 006-0. 008
<0. 004-0. 036
0.019-0.070
0.14-0.82
—
0.055-0.094
0.015-0.092
0.012-0.12
—
0.010-0.090
4.67-8.08
a. Range of twelve 24 h composite
b. Average of three 24 h composite
Jul 1976b
624
173
—
42.2
22.2
19.4
0.7
12.7
12.7
2.2xl06

• •

7.0
1197

1847
--
--
1.1
0.6
246
59
62
11.3
258
176
0.007
<0.01
<0.1
0.004
0.008
<0.004
0.024
0.41
0.001
0.069
0.063
0.016
<0.001
0.015
5.33
samples.
samples.
Sep 1976b
593
208
--
46.7
33.5
12.9
0.34
10.7
9.0
ISxlO6

6.7xl06

7.3
1559

2400
--
«
1.8
0.57
377
71
57
12.1
471
219
<0.006
<0.01
<0.1
<0.001
0.011
O.004
0.011
0.17
<0.001
0.064
0.086
0.033
<0.001
0.032
8.08

Dec 1976b
659
153
—
30.9
22.3
8.1
0.5
16.2
10.6
69xl06

11.6xl06

7.8
1033

1620
221
433
1.4
0.37
230
53
75
12.6
192
243
0.011
<0.001
0.11
0.007
<0.006
0.015
0.068
0.34
•cO.OOl
0.068
0.015
0.068
<0.001
0.053
4.76

Mar 1977b
946
346
248
40.9
23.2
17.5
0.18
10.2
9.8
21X106

19X106

7.2
1044

1293
327
459
1.3
1.08
197
31
63
15.6
216
215
<0.007
<0,01
0.21
0.003
<0.006
0.032
0.032
0.63
<0.001
0.080
0.040
6.10
<0.001
0.09
4.67

Average
706
220
248
40.2
25.3
14.5
0.43
12.4
10.5
27.6xl06

12.4xl06

7.3
1208

1790
274
446
1.4
0.66
262
54
64
12.9
284
213
<0.008
<0.01
<0.13
<0.004
<0.008
<0.014
0.034
0.39
<0.001
0.070
0.051
0.054
<0.001
0.048
5.71

                         20

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CONSTITUENT LOADING

     Wastewater characteristics that can  influence  the  amount of  wastewater
that can be applied to a rapid infiltration  site  include  organic  matter,
nitrogen, and phosphorus.  The current constituent  loading  rates  at the
Hoi lister rapid infiltration site are shown  in  Table  6.

                TABLE 6.  WASTEWATER CONSTITUENT  LOADING  RATES
                                    kg/ha
Constituent
COO
BOD
TOC
Total nitrogen
Total phosphorus
Daily
304
95
107
17.3
5.3
Annual
111,000
34,500
39,000
6,310
1,950
                         a.  Based on 438 L/s applied to 8.8 ha.

                            kg/ha x 0.89 = Ib/acre
     The mechanisms which cause removal  and  the  fate  of  these  and other
constituents during infiltration and  percolation through the  soil  profile are
discussed in the soil and groundwater investigation sections.
                                      21

-------
                                   SECTION  6

                                  METHODOLOGY
 SITE  SELECTION

      The  initial  phase  of  the  project  involved the selection of a suitable
 study site.  The  1974 EPA  Municipal Waste Facilities Inventory for California
 was used  to compile  the  initial  list of candidate sites [8].  Listed in the
 inventory were  a  total  of  106  sites where some variation of the rapid
 infiltration process was being used (see Table 7).

      The  candidate sites were  screened according to criteria established by
 the EPA and additional  criteria  developed during the screening procedure.  To
 be considered,  a  site must have  been representative of the current state-of-
 the-art for rapid infiltration treatment as defined by the site screening
 criteria  shown  in Table 8.

      The  initial  screening was performed using in-house information, an EPA
 list  of existing  land application systems [2], and information obtained by
 telephone.  The most promising sites were identified for more detailed
 screening, and the number of candidate sites was systematically reduced.
 Detailed  screening resulted in a list of the six most promising sites, shown
 in Table 9.   Three promising sites that currently pretreat to the primary
 level  and three that pretreat to the secondary level  before land application
 were  selected.

      Hoi lister was chosen as best representing the soil  and groundwater
 conditions for a  typical site with potential  for treatment by rapid
 infiltration where primary effluent is applied.   Fontana was rejected because
 the depth to groundwater was very large and the  direction of groundwater  flow
was difficult to define.  Gilroy was rejected because  of shallow groundwater
 and significant influence from a nearby industrial  wastewater land
 application system.

      Of the systems applying secondary effluent, Santee was selected as
 having a  well-defined groundwater flow pattern.   Santa Maria was rejected
 because of operational changes in the last few years.   Oakdale was rejected
 because of shallow groundwater.

      The  final screening step included visits to the two most promising
 sites--Hollister  and Santee.  It was concluded that the most suitable site
 for the purposes  of this study would be the one at Hoi lister, California.
 The principal  reasons for selection were that Hoi lister was more typical  of
most  rapid infiltration systems, had a longer history  of operation,  and was
applying primary effluent.
                                     22

-------
               TABLE 7.  LIST OF CANDIDATE  RAPID INFILTRATION SITES
                         IN CALIFORNIA,  OCTOBER 1975 [8]

1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
Community
Altaville
Anderson
Arden
Atascadero
Avenal
Big Bear Lake
Brea
Buellton
Buttonwillow
Calpella
Camarillo
Capistrano Beach
Cawelo
Ceres
Chester
Corona
Crescent! a
Dixon
Hemet
Escalon
Etna
Fillmore
Firebaugh
Fontana
Ft. Jones
Fresno Co. 1
Fresno Co. 2
Gilroy
Gridley
Gustine
Healdsburg
Hollister
Hughson
Irvine Ranch
Jamestown
Jurupa
King City
Lake Arrowhead
Lakeport 1
Lakeport 2
Lakeside
Laton
Lodi
Lone Pine
Lechuza
Malibu
Trancas (L.A. Co.)
Whittier Narrows (L.A. Co.)
L.A. Co. SD #22 Azuza
Manteca (Raymus Village)
Mariposa
Mendota
Mills-Cordova
Flowrate,
L/s
<3.1
33
298
11
20
8.8
2.2
3.5
<3.9
<0.9
114
17
<5.3
42
13
136
0.9
13
140
8.8
4.4
18
13
101
4.4
8.8
0.9
101
39
48
22
35
12
153
4.4
39
18
22
8.8
4.4
24
4.4
197
8.8
<4.4
<4.4
<4.4
661
31
0.4
2
4.4
8.8

54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
88.
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
Community
Montalvo
Mount Shasta
Nevada City
Oakdale
Oceanside La Saline Plant
Oceanside Buena Vista Plant
Oceanside San Luis Rey Plant
Olivehurst
Orange Co. SD Plants 1 and 2
Moulton Miguel Plant 2A
Orange Cove
Palm Springs
Perris
Pinedale
Pixley
Sierra Lakes
Squaw Valley
Porterville
Rainbow A
Rainbow B
Rainbow C
Rancho Santa Fe
Redding (Clear Creek)
Redding (Paso Land Co.)
Redlands
Reed ley
Rialto
Richvale
Rio-Dell
Ri pon
Riverbank
Salida
San Clemente
San Diego Co. (Brown Field)
San Miguel
Santa Maria
Santa Paula
Santee
Shasta Co.
Soledad
Spreckels
Tahoe City
Tranquillity
Tuolumne
Visalia
Waterford
Westwood
wheat! and
Winters
Winton
Woodbridge
Yreka
Yuba City
Flowrate,
L/s
4.8
22
31
48
140
18
22
33
6790
11
11
131
13
11
4.4
2
0.4
75
0.35
0.4
0.2
3.2
158
1.8
110
35
92
1.3
15
13
13
18
88
1.3
1 .3
188
74
44
0.09
13
3.5
5.2
2.2
4.4
210
8.7
8.7
5.7
8.8
15.0
8.8
44
66
L/s x 0.0228 = Mgal/d
                                       23

-------
    TABLE &.   INITIAL  SITE  SCREENING CRITERIA
            Item
                                         Criteria
Period of operation
PreappHcation treatment
Wastewater sources
Wastewater flowrate
Uastewater application rate
Depth to  groundwater
Control site

Availability of data

Operation practices
>10 years
Remain unmodified for at least 10 years
Domestic and commercial8
>4.3 L/s
>6 m/yr
>3 m and <30 m
Comparable geohydrologic characteristics
within 1.6 km of site
Historical Wastewater and groundwater
quality must be available for comparison
purposes
1.  Wastewater application to the       b
    spreading basins  must be Intermittent
2.  Sludge must never have been applied
    to the spreading  bas1nsc
3.  Soil conditions 1n the basins should
    not have been altered drasticallyd
a.  Industrial wastewater. in small amounts, resembling municipal
    wastewater is acceptable.
b.  Systematic flooding and drying over several  days with
    multiple independent basins.
c.  .Constituents are generally much more concentrated in sludge
    than  in wastewater.
d.  Surface disking or scarifying to restore infiltration is  normal
    and acceptable.
L/s x 0.0228 = Hgal/d
m x 3.281 = ft
km x 0.621 = mi
   TABLE  9.   SUMMARY  OF DESIGN AND OPERATING
         INFORMATION  ON SIX CANDIDATE  SITES
Site
location
Hoi lister
For) tana
Gllroy
Santee
Santa Maria
Oakdale
Level of
pretreatment
Primary
Primary
Primary
Secondary
Secondary
Secondary
Flowrate,
L/s
44
no
118
44
241
61
Application
rate, m/yr
15
21
24
58
46
67
Duration of
operations,
total number
of years
30
20
52
17
14
10
  a.  Average annual applied wastewater volume divided by the
      Infiltration basin area.
  L/s x 0.0228 * Hgal/d
  »/yr x 3.281 « ft/yr
                              24

-------
STUDY PLAN

     The method of approach was to characterize the applied effluent,
groundwater, and soil over a 1 year period.  The concentration of
constituents in the effluent and groundwater were compared to determine the
current efficiency of the soil matrix in removing constituents contained in
the effluent.  The constituents of concern in groundwater were those for
which drinking water standards have been set, and those that affect water
quality for irrigation and livestock watering.  Observation wells
constructed for sampling groundwater were shown in Figure 3.

     A mass balance of selected soil constituents was perfonned to determine
the effects of 30 years of effluent application to the land on the ability
of the soil to retain constituents in the applied effluent.  For soils, the
important constituents were those that could restrict the use of the site to
grow crops if the current land use were changed.  The soil sampling
locations are shown in Figure 4.

     A sampling program was devised to quantify the constituents in the
effluent, groundwater, and soils.  Certain constituents, specifically
organic pesticides and viruses, were not measured because of the inordinant
expense involved in the analyses.  A summary of the sampling program is
given in Table 10.

                   TABLE 10.  SUMMARY OF SAMPLING PROGRAM
Feature
Total No. of samples
No. of constituents
per sample
Timing of samples
Type of samples
Effluent Groundwater
12 52
38 37
6/76, 9/76, 6/76, 9/76,
12/76, 3/77 12/76, 3/77

Soil
20
25
6/76,
3/77
               No. of sampling
               locations                1         13          5

               Type of samples         24 h       Grab       Multiple
                                  composite                composite
                                      25

-------
                                                 INFILTRATION  I
  DRY
I  SLUDGE  \
  STORAGE -J/
 SLUDGE
 BEOS
 DIGESTER
 CLARIFER
 EQUALIZATION
 RESERVOIR
  INFILTRATION
     BASINS
0 50 100 200  300  400  FEET
  II    I     I
                                                                METRE
     SOIL SAMPLING  LOCATION -  ONSITE

A  SOIL SAMPLING  LOCATION -  CONTROL
  Figure 4.   Proposed  soil sampling locations at the  Ho "Mister
              rapid infiltration  site.

                             26

-------
 Effluent  Sampling

     The  primary effluent was sampled through the use of a 24 hour
 refrigerated,  composite  sampler.   The sampler was battery-powered and
 installed to take  samples from the effluent trough of the clarifier.  Each
 sampling  day (3 consecutive days)  produced 24 separate 1  hour samples that
 were composited according to flow.  The constituents analyzed for were:

         Organic: COD, BOD, TOC
         Bacterials:  Total coliforms, fecal coliforms
         Minerals:  Suspended solids, IDS, pH, alkalinity, conductance, N organic,
                 N03-N, NH3-N, total N, total P, P04-P, Na, K, Ca, Mg, Cl, S04, B, F
         Trace elements:  Ag, As, Ba, Cd,  Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, In


 Groundwater Sampling

     Sampling  of the  groundwater took place in the specially constructed
 series  of observation wells and five existing offsite wells.  Observation
 wells at  Sites 1,  2,  and 3 were drilled to intercept the subsurface flow
 leaving the site.   Observation well No. 4 was drilled as an offsite control
 well.   Observation well  No. 5 was  drilled near the center of the site to
 intercept the  top  of  the groundwater mound created by the infiltrating
 effluent.

     At the peripheral well locations (Nos. 1 through 4), the first drilling
 was to  approximately  54  m (180 ft).  At 1.5 m (5 ft) intervals the drilling
 was stopped and a  splitspoon sample of material was taken.  This allowed the
 profile to be  plotted by the geologist.  A well screen was established if
 water bearing  material existed near the 54 m (180 ft) level.  A second well
 was drilled to either an intermediate depth (24 m = 80 ft) or a shallow
 depth (8  to 11 m = 25 to 35 ft)  or both.

     It was originally estimated that four shallow wells (noted as "A")
 three intermediate wells (noted as "B"), and four deep wells (noted as "C")
 would be  drilled.   The location of potential  water bearing material in the
 soil profile was determined at each site from a log of each boring.
 Observation wells  were established where potential water bearing material
 was found.  The locations of the shallow,  intermediate, and deep wells
 established from the  geologic investigation were shown in Figure 3.

     The wells were equipped with  pumps to obtain groundwater samples from
 specified  depths.  Sampling pumps  were hand crank portable plunger pumps
with a  flush cap cylinder,  rod  and couplings, and PVC carrier pipe.  The
 flush cap  cylinder was smaller  in  diameter than the 5.1  cm (2 in.)  inside
diameter of the observation wells.   The pumps were able to obtain sufficient
water volume to flush out the standing water column in a relatively short
period  of  time.  Samples  were withdrawn in June 1976, September 1976
December 1976, and March  1977 (see Table 10).
                                     27

-------
      Five existing offsite wells were monitored  at the  same  frequency.  An
 upstream well  (No. 4C)  owned by the  City  of  Hoi lister served as a
 supplementary  control well.   Four downstream wells (6C,  7C,  8C, 9C) 0.8 km
 (0.5 mile)  away were also  utilized.   These privately owned wells are used
 for irrigation and domestic  water production.

      Suspended solids content was measured selectively  in the groundwater
 and carbon  chloroform extract (CCE)  was not  measured in  the  primary
 effluent.   All  other constituents measured in  the  groundwater were the same
 as  for  the  applied effluent.   The methods of analyses for both effluent and
 groundwater samples were from Standard Methods [9].

 Soil  Sampling  and  Analytical  Procedures

      The soil  profile was  sampled at three onsite  locations  and two offsite
 control  locations  (Figure  4).   The onsite locations were in  a spreading
 basin that  has  been in  use since  about 1946.  Onsite and control samples
 were  taken  in June 1976, and  again in  March  1977.  To establish the extent
 of  vertical  variation in soil  chemistry within the soil  profile, samples
 were  taken  at  the  following  depth increments:

                        Depth
                      increment
                         No.          cm        (ft)

                           1          0-2       0-0.07
                          2          2-4    0.07-0.13
                          3          4-16   0.13-0.52
                          4         25-35    0.8-1.2
                          5         95-105   3.1-3.4
                          6        295-305   9.7-10.0


      Composite  samples were taken at each sampling location  to  account for
 the spatial variability of the soil.   The basin selected for sampling  was  18
 by  180 m (60 by 600 ft); accordingly, grid nodes  spaced  1.5  by  3 m (5  by 10
 ft) were established at each sampling location.  For the first  three depth
 increments, 20  subsamples were taken for compositing (one per grid node).
 Preliminary analyses indicated that increments 1  through 3 were
 indistinguishable  and were finally analyzed as one composite whose mass was
 proportional to its volume.  For the  fourth depth increment  at  the onsite
 locations, 10 subsamples were composited.   The fifth and sixth  increments  at
 the onsite locations and the last three depth increments at  the control  site
were  sampled from trenches  excavated  by backhoe.   Three  equally spaced
 trenches were excavated  over the original  grid nodes.  Samples  were taken
 from  each face of a trench  to duplicate as closely as possible  the 10
subsample locations of the  fourth depth increment.   The  high  soil  density  at
the control  site made it necessary to take subsamples from the  excavated
trench sidewalls rather  than sampling from the surface for the  last three
increments.
                                     28

-------
     To determine  if any significant quality changes have resulted from the
application of wastewater,  23 chemical and 3 physical  soil  parameters were
monitored at both  control  and treatment  site locations.   All laboratory
analyses were performed on  air-dried soils.  A  listing of the constituents
and analytical procedures is shown in Table 11.   The only field performed
measurements were  bulk density and infiltration rate.

             TABLE 11.   SOIL CONSTITUENTS AND ANALYTICAL  PROCEDURES
Procedure
Laboratory
pH [11]
Conductivity [11]
Organic Matter [12]
CaC03 [12]
Nitrogen [11]
B [11]
Total-P [11]
Extractable-P [11]
CEC [11]
Exchangeable cations [11]
Metals [13]
Particle Size [11]
Field
Infiltration Rate [11]
Bulk Density [11]
Description

1:1 paste
Saturation extract
K2Cr20?-H2S04
Gravimetric HC1 loss
Kjeldahl
Hot water extract
HC104-digest
0.5 m NaHCOj
NH4OAc - pH 7
NH4OAc - pH 7
DTPA-TEA extraction3
b
Sieve-hydrometer

Double cylinder
Core, excavation
Soil fraction

All
All
<0.6 mm
<2 mm
<2 mm
<2 mm
<2 mm
<2 mm
<2 mm
<2 mm
<2 mm
All

—
~
                a.  0.005'M diethylenetriamine pentacetic acid - 0.1 M tri-
                   ethanolamine - 0.01 m CaCl2, 40 mL/20 g soil.

                b.  Clay analyses performed at discretion of laboratory
                   technician.

                mm x 0.03937 = in.
                m x 3.281 = ft.
      Additionally,  phosphorus sorption  experiments were conducted  in the
 laboratory  on both  control and treatment site samples.  Sorption isotherms
 were determined using a "non-destructive"  method developed by  Enfield and
 Bledsoe [10].
                                       29

-------
                                   SECTION 7

                          HYDROGEOLOGIC  INVESTIGATION
      The rapid infiltration site  at Hoi lister  is on  the flood plain of the
 San Benito River,  approximately 150 m  (500  ft)  south of the river channel.
 The flood plain is typically flat and  has been  used  as a source for
 commercial  sand and gravel  in the Hoi lister area.  The site is protected
 from flooding  by fill  materials.

 REGIONAL HYDROGEOLOGY

      The site  is located  in the San Juan subbasin of the Gilroy-Hollister
 groundwater basin.   The San Juan  subbasin is defined within the larger basin
 by  geologic features that form relatively impermeable barriers to
 groundwater movement.  These boundaries include the Sargent anticline in the
 Lomerias Muertos and Flint  Hills  on the north, the Calaveras fault and the
 Bird Creek  Hills on the east, and the  San Andreas fault zone on the
 southwest (Figure  5).

      The San Juan  subbasin  is further  divided into subunits.  The boundaries
 of  these subunits  are minor faults  or  zones of deformation that create
 hydraulic discontinuities.   The location of the Hollister site relative to
 nearby subbasin  and subunit boundaries is illustrated in Figure fa.

      The major  aquifer in the San Juan subbasin consists of unconsolldated
 to  weakly-cemented  alluvium  of Holocene Age and the upper member of  the
 Purisima formation  of Pliocene age.  These units are Indistinguishable on
 drillers' or electric logs  and are  generally believed to form  a single
 hydrologic  unit.  Within these sediments,  the most productive  water-bearing
 units are lenticular beds of sand and gravel that are interbedded with silt
 and  clay  layers.  Groundwater occurs mainly under artesian  or  semi-artesian
 conditions  in these units, although locally perched or semi-perched
 conditions may exist.

     The  Holocene alluvium is broken by currently active faults 1n places,
 such as  the one that occurs approximately  600 m (2,000 ft)  west of the
Hollister site.  The Purisima formation and  the older Tertiary  and Jurassic
 rocks that underlie the alluvium  are highly  folded  and have  been extensively
broken and tilted by faulting. Electric logs of gas  production wells  in  the
Lomerias Muertos indicate  that poor quality  groundwater occurs  to depths  of
200 to 300 m (600 to 1,000 ft) in  the older  formation.
                                     30

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	 SUBBASIN BOUNDARY


•— SUBUNIT BOUNDARY
                     0  1   2  3  4   5
                                           5 MILES
                                              KILOMETRES
    Figure 5.  Relation  of groundwater subbasins and
          subunits to  geologic features  [14].
                           31

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CO
ro
                                                                                  	55	198S IATE»-LE»EL


                                                                                                    CONTOURS , •
                                                                                   0    1000    2000 FEET

                                                                                   I  I  I   < I  I  '

                                                                                   0          300    METRES
             l>
                            Figure 6.   Hydrogeology of the Hollister rapid  infiltration site [14],

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     Within the main groundwater  body  of  the  San Juan  subbasin, groundwater
flow is toward a major pumping  depression in  the south-central part of the
valley.  This depression has  been caused  by the withdrawal of groundwater
for irrigation in amounts that  are in  excess  of the  rate of recharge,
particularly since 1945.  Throughout the  valley, groundwater levels declined
up to 30 m (100 ft)  between 1913-1968,  and the San Benito River changed from
a gaining to a losing stream.  Currently, all discharge from the San Juan
subbasin is by wells, and recharge consists of stream  infiltration and
direct infiltration of precipitation.   During the period 1946-1969,
discharge exceeded recharge by  an average rate of 123  L/s (2.9 Mgal/d) [15].
However, even with current drought conditions, which began in 1975,
groundwater levels in the main  aquifer did not decrease during the study
period, beneath the rapid infiltration site.

OBSERVATION WELLS

     Existing information concerning the  subsurface  hydrology near the site
was reviewed and used as a basis  for locating the groundwater monitoring
wells.  This information included a U.S.  Geological  (USGS) survey report on
the regional subsurface hydrology [14], drillers' logs for wells near the
site, and the logs of two shallow test borings completed at  the site [16].

     Examination of these data  sources indicated that  the principal aquifer  in
the vicinity of the Hoi lister site is  alluvium and  the upper member of the
Purisima formation.  The principal aquifer is underlain by the  middle member
of the Purisima formation, a  less permeable  unit that  contains saline water  at
depth and water with a high concentration of  sulfate locally.  The base of  the
fresh water lies between 100  to 200 m  (300 to 600 ft)  below  the surface in  the
Hoi lister area.

     The water level contours shown in Figure 6  indicate  that groundwater
beneath the site moves  in a westerly  direction  toward  the  subunit boundary
west of the site.  These water level  contours are  based on wells  screened  at
depths between 30 to 100 m (100 to 300 ft).

     Four peripheral sites and one site within  the  effluent  treatment  area
were chosen to monitor  groundwater levels and quality.  Monitoring  sites were
located close  to the infiltration basins so that extraneous  effects  on
groundwater quality, such as former or existing  upgradient waste  loadings,
irrigation, streamflow  infiltration,  etc., could be eliminated.

     Observation well sites 1,  2, and 3  (see Figure 7) were  located  on  the
downgradient  side of the site to  intercept the  suspected path of  groundwater
flow from the  infiltration basins.  It was proposed that a shallow,  an
intermediate,  and a deep observation well be constructed at  each  of  these
three  sites.   The shallow wells would have a 0.6 m (2  ft)  length  of  screen  set
at a depth between 3 to 13 m (  10 to  40 ft),  and the intermediate  and  deep
wells would have similar screens  set between 20 to  30 m (60  to 90  ft)  and  45
to 60 m (135  to 180 ft), respectively.  Available information indicated  that
the movement  of percolates through the unsaturated zone might be  complex  and
that perching  conditions might be caused by apparently extensive  clay  deposits
which  underlie  the  site at depths of less than 10  m (30 ft).   The  shallow


                                      33

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    RAPID INFILTRATION
          BASINS
                                               GROUND SURFACE
                                               CONTOURS. •
                              0    250   900
                                                  1000 FEET
                                                 	I
                                    100
                                           T
                                           200
                                                  300  METRES
Figure 7.   Observation well  sites.

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wells at each site were proposed to reveal  possible perching  in  the
unsaturated zone, while the intermediate and deep wells  were  intended  to
define the movement of the effluent in the  saturated zone.  The  number of
wells that were actually placed at each site was limited by the  availability
of permeable materials within the desired depth intervals.

     At Site 4, the City of Hoi lister supply well was proposed as  a  deep
monitoring well upgradient from the treatment site.  Therefore,  only
intermediate and shallow wells were proposed at this site.

     Site 5, located in infiltration basin  6, was proposed as a  shallow
observation well that would intercept the groundwater mound created  by
infiltration of the applied effluent.

     The construction of the observation wells at Sites  1, 2, and  3  proceeded
as follows:

     1.   A 12.7 cm (5 in.) diameter uncased boring was  drilled  to the
          maximum scheduled completion depth using a Failing  750 rotary
          drilling rig.  The completion depth depended on the depth  at which
          permeable materials were encountered in the 45 to 6U m (135  to 180
          ft) interval.

     2.   During the drilling, 3.5 cm (1.4  in.) diameter splitspoon  samples
          were taken at 1.5 m (5 ft) intervals throughout the boring.   The
          location of permeable zones was observed by a  geologist  as the
          samples were taken.

     3.   Upon completion of the boring, depths for observation  wells  were
          selected based on availability of permeable zones.   If feasible, a
          deep observation well was installed in the borehole.  The
          observation wells were constructed with lengths of  5 cm  (2 in.)
          diameter PVC pipe with a 0.6 m (2 ft) length of 10-slot  PVC  screen
          (0.03 cm, 0.01 in., openings) at  the bottom.  The space  between
          the well and the open hole was filled with sand at  the screened
          interval and with bentonite slurry from the top of  the screen
          to the surface.

     4.   Similar intermediate and shallow  boreholes were then drilled to the
          chosen depths for the installation.  These wells were  within 1.5 m
          (5 ft) of the original boring, and splitspoon  samples  were
          therefore not taken.  A drawing of a typical completed observation
          well and a photograph of a drilling rig are shown in Figure  8.

     At Sites 4 and 5, borings were drilled only as far  as the intermediate
and shallow zones, respectively.  Similar observation wells were then
installed in the boreholes.

     Pertinent information concerning observation well construction  is
contained in Table 12.  Shallow, intermediate, and deep  wells are  indicated
by the letters A, B, and C, respectively.  Examination of the table  shows
that,  of the original  12 proposed observation wells, 4 could  not be  completed
due to the extensive clay layers beneath the site.

                                     35

-------
                                                3B 5ci
                        10P Of PIPE
                   BEN1DNIIE SUR«r
                                                   GROUND SUIMCE
                   TOP OF SAND FILTER
                                            • Pvt IELL ICKllK - NO. 10 1LOI
                                             (CLOIEO IOTION)

Figure  8.   Typical  observation well construction and drilling rig,
                                      36

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                TABLE 12.  OBSERVATION WELL CONSTRUCTION DATA
Well No.
1A
IB
1C
2C
3A
3B
4B
4Ca
5A
Screened
interval ,
m below
land surface
5.5-6.1
23.8-24.4
48.2-48.8
54.3-54.9
7.0-7.7
20.7-21.3
17.7-13.3
56.4-59.4
10.1-10.7
Elevation Elevation of
of land measuring point
surface, (top of casing),
m m
73.7
73.7
73.7
72.1
73.0
73.0
73.8
—
72.8
74.2
74.2
74.2
72.7
73.4
73.5
74.6
74.4
73.4
                   a.  Existing City of Hoi lister well.
                   m x 3.281 = ft.

SITE HYDROGEOLOGY

     Graphic logs of the borings at Sites 1  through 5, which are north-south
and east-west geologic cross-sections through the site area, are shown in
Figures 9 and 10.  These graphic logs were based on the geologist's
description of the samples.  The logs for two test borings that were done in
a previous investigation and a driller's log for the City of Hoi lister supply
well (observation well 4C) are also shown.  Although it is evident that the
nature of subsurface materials was highly variable both horizontally and
vertically, several generalizations can be made.

     A layer of permeable material generally occurs from the surface to a
depth of 10 to 20 m (30 to 60 ft).  A clay layer separates this upper
permeable zone from an intermediate one which occurs at depths between 15 to
25 m (45 to 75 ft).  All of the deep logs showed a thick zone of silt and clay
beneath the intermediate permeable zone.  A deep zone of permeable material
also occurs below depths of 55 to 65 m (165 to 195 ft).

     The depth to water in the observation wells that.were constructed at the
site varied from 10 to 20 m (30 to 60 ft) below the surface.  The water level
elevations, on 4 selected days, in the six observation wells that were
screened in the saturated zone are shown in Figure 11.  At each individual
site, the water level in the intermediate zone was higher than that in the
deep zone, indicating a net downward movement of water.  This movement of
water may be in response to the pumping from the city well (4C) in the deep
aquifer.
                                    37

-------
         SITE  1  TB-1
                                                                    SITE 5
                                                                                              SITE 3   TB-2
U>
00
    14.2
19. g
22.0
     21 .0
     93. 1
                                          INTERMEDIATE PERMEABLE ZONE
                                          WELL-INDURATED  CLAY  AND  SILT
20. 4 i
                                                                                           29.6
                                                                                           39.4
                                          NOR IZONTAL SCALE :   1  c• - 40 »
                                          VERTICAL SCALE:  AS  SHOWN. •
                                                                        or  1  i n . - 330  It
                                                                                                                  LEGEND
                                                                                                            13.7
                                                                                                            1 4. e
                                                                                                                        SCREEN
                                                                                                                        GRAVEL
                                                                                                                        COARSE
                                                                                                                         SAND
                                                                                                                        MEDIUM
                                                                                                                         SAND
                                                                                                                    FINE
                                                                                                                    SAND
                                                                                                                        SILT
                                                                                                                    CLAY


                                                                                                                    MUCK.
                                                                                                                    PEAT
                                                                                                                  DEPTH OF

                                                                                                                  CON TACT.•
                                              Figure  9.  North-south geologic  section.

-------
           SITE  2
                                                                                              SITE 4
        2. 1
       13.1
       18.0

       20. 7
..
LC
      54.3
      55.4
                                          SHALLOW PERMEABLE  ZONE
                                             CLAY AND SILT
        INTERMEDIATE  PERMEABLE ZONE
                                            •ELL-INDURATED  CLAY AND SILT
                                           DEEP PERMEABLE ZONE
HORIZONTAL  SCALE:   1  cm = 40  m
VERTICAL  SCALE:  AS  SHORN  m
                                                                                        8 . 2
                                                                                      -12.2
                                                                                      15.8

                                                                                      24.7
                                                                                      43.3
                                                                                      44.2
                                                ,	49. 1


                                                 53.1

                                                56 . 4

                                                59 . 9
                                                                  or  1  in  =  330  ft
                                                                                     6 5 . 0
                                                                                               6 . 7
                                                           l_2. 2
                                                           I 3 .
ii a.
It a. s
                                                                                              25.0
                                                                                              29.6
                                                                                              31.0
                                                                                                                 LEGEND
                         SCREEN

                         GRAVEL
                                                                                                                      COARSE
                                                                                                                       SAND
                                                                                                                      MEDIUM
                                                                                                                       SAND
                                                                                                                      FINE
                                                                                                                      SAND
                                                                                                                 =   SILT
                                                                                                         13.7
                                                                                                         14.6
                                                                                                                     CLAY
                                                                                                                     MUCK .
                                                                                                                     PEAT
                      DEPTH  OF
                      CON TACT.m
                                          Figure  10.   East-west geologic  section.

-------
                                                         NOTE:  PATUM GROUND SURFACE
                                                               ELEVATION= 73n
                                                               FOR ALL WELLS SHOWN
1 2
                                        ©           ©
              0           0
1 S
IS
              $          *
              ©
                                                     J
             6/28/76
                         11/22/78      1/28/77        4/1/77
                 Figure 11.   Water levels  in  intermediate  and
                deep observation wells on  four selected  days.
                                      40

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     Within the intermediate zone, the water levels indicated a movement of
water  toward the northeast.  This trend may have been caused by greater
leakage from the intermediate to the deep aquifer in the vicinity of Site 3,
since  the impermeable clay between the two zones appeared to be thinner to the
north.

WATER  LEVEL RESPONSE

Shallow Wells

     The shallow observation wells at Sites 1, 3, and 5 were installed to
determine if perching conditions occurred as effluent percolated down to the
clay layers that underlie the upper permeable zone, as well  as for monitoring
water  quality.  Early in the 1 year study period it was discovered that Wells
3A and 5A had rapid water level  responses to effluent application.  Continuous
water  level recorders were installed to more accurately define these responses
(Figure 12).  Well 1A remained dry throughout the study, except when the berni
around Basin 20 was breached and the field adjacent to Site 1 was flooded.

     The response of Wells 3A and 5A to wastewater application is shown in
Figure 13.  The water level in Well 3A rose at a greater rate and to a higher
elevation than that in Well 5A.   This difference was most likely a result of
higher permeabilities in the upper layer in the vicinity of Site 3 which
created a preferred path for infiltration.  After the peak water level  was
reached, the perched water table dropped at a greater rate in Well 3A than in
Well 5A, again indicating a higher aquifer permeability at Site 3.

     Both of these wells responded noticeably to flooding in Basins 3 through
8 since these basins were closest to the two wells.  The effects of flooding
in other basins were not evident on the hydrographs of Wells 5A or 3A.

     It was also observed, during the study, that the rise of water levels in
Wells  3A and 5A was related to the depth of the effluent applied in the
infiltration basins.  A specific relationship between these phenomena was
beyond the scope of the project, however.

Intermediate and Deep Wells

     The intermediate depth observation wells constructed at Sites 3 and 4
showed no discernable response to effluent application.  The wells did show a
yearly water level  fluctuation of 0.7 to 1.0 m (2 to 3 ft) ,  with high water
levels occurring during the winter when natural  recharge is greatest (Figure
     The intermediate well at Site 1  showed a 1  m (3 ft)  rise in water level
at the same time that the water level  in Well 1A rose.  This occurred when
the adjacent field was flooded following a breach in the Basin 20 berm.   The
boring log from Site 1 showed 15 m (50 ft) of clay and silt between the  upper
and intermediate permeable zones.  The most probable explanation for the
response of Well IB is that there were zones of  permeable material  nearby
that allowed the effluent to pass down to the intermediate zone.
                                     41

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Figure 12.   Continuous water level  recorders,



                     42

-------
   1 . 5
   4 . 5
   6 . D
   7 . 5
   9 . 0
                                 , 54 ~- BASIH(S)  BEING FLOODED
                     DRY
                                                        LEGEND
                                                               WELL  3 A
                                                               WELL  5 A

20 24 28
All G
^ ^/
' 4 8 12 18 20 24 28 4 8 12 IB 20
SEP 0 C T
                                TIME. DAYS  (1976)
   4. 5
   8. 0
   7. 5
   9.0
  10. 5
                       1  3 5 7
                      i.ViVi
                                                                     3 5  e
            .J
                                                                      I	1	1
     20  24   21
           0 C T
4    8   12   1620  24   28
           N 0 V
                               4    8   12  18   20
                                       DEC
   4. 5
C"  8.0
ui  g . o
u. 10. 9
     24   28
        DEC
      (1978)
                                TIME. CAYS  (1976)
>    12   16   20  24  28  |
      1 AN
                     (1977)
            TIME.  DAYS
                                I   12   18   20
                                     f I I
                                                                      24   28
          Figure 13.  Water  level  response to basin  flooding in
                       observation wells 5A and 3A.
                                    43

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JUL
                                                                                 APR    HAY
                                        TIME. Months
                                                                     1977
     Figure 14.   Water level response  of intermediate and deep wells.

-------
     The deep observation wells,  at Sites 1,  2,  and  4,  did  not  show any
response to effluent application  (Figure 14).   The lower  permeable zone  in
which these wells were screened is overlain by a 20  to  35 m (60 to 110 ft)
layer of clay and silt that is probably continuous beneath  the  treatment  site
and is an effective barrier to vertical  groundwater  movement.

SUMMARY AND DISCUSSION

     The data collected during the observation well  drilling and  the water
level monitoring indicated that the movement of the  effluent through the
ground is complex and controlled  by highly variable  subsurface  conditions.
The intermediate and deep observation wells showed no response  to effluent
application in the infiltration basins.  The infiltrating effluent apparently
formed a mound which is perched on top of the clay layer  that underlies  the
upper permeable zone.  When infiltration ceased, the mound  subsided mainly by
lateral outflow from the site.

     The mound that resulted from the flooding of Basins  3  through 8 was
dissipated by lateral outflow to  the north.  The hydrograph of  Well 3A
indicates that Site 3 is in a more permeable part of the  upper  zone, which is
probably a preferred path for infiltration and outflow.  The exit paths  for
mounds created by flooding other  basins are not known,  although it is
probable that the outflow was to  the north for Basins 1 through 13 and toward
the south or southeast for Basins 14 through 20.  No lateral outflow was
observed at Site 1, southwest of  the treatment facility.

     Some vertical leakage may be occurring also, especially near Site 3
where the clay and silt between the upper and intermediate  permeable zones is
the thinest.  A comparison of iron and manganese concentration  in effluent
and groundwater indicates that vertical  leakage from the  upper  to the
intermediate permeable zone is taking place (see Section  9).
                                    45

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                                  SECTION B

                              SOIL INVESTIGATION
INTRODUCTION

     The Hoi lister rapid infiltration site is located in an area mapped  as  a
Metz sandy loam [3], though the terms gravelly sand or gravelly sandy  loam
appear more appropriate.  The soil is alluvial, currently classified as  a
Typic Xerorthent [3].  A representative profile is shown below [3]:

     Ap   0 to 20 cm (0 to 8 in.), grayish-brown (2.5 Y 5/2) sandy loam, very
          dark grayish-brown (2.5 Y 3/2) when moist; weak, fine granular
          structure; soft when dry, very friable when moist, nonsticky and
          nonplastic when wet; few very fine and fine roots; few very  fine
          and fine tubular pores; mildly alkaline; slightly effervescent;
          clear, smooth boundary.

     Al   20 to 35 cm (8 to 14 in.), grayish-brown (2.5 Y 5/2) sandy loam,
          very dark grayish-brown (2.5 Y 3/2) when moist; weak, fine
          subangular blocky structure; soft when dry, very friable when
          moist, nonsticky ana nonplastic when wet; few very fine and  fine
          roots; few very fine and fine interstitial pores; moderately
          alkaline, slightly effervescent; clear, wavy boundary.

     Cl   35 to 108 cm (14 to 43 in.), light brownish-gray (2.5 Y b/2) sand,
          grayish-brown (2.5 Y 5/2) when moist; single grain; loose when dry
          or moist; nonsticky and nonplastic when wet; mildly alkaline,
          slightly effervescent; abrupt, smooth boundary.

     C2   108 to 150 cm (43 to 60 in.), light brownish-gray (2.5 Y 6/2)
          stratified coarse sand and gravel, grayish brown (2.5 Y 5/2) when
          moist; single grain; loose when dry or moist, nonsticky and
          nonplastic when wet; contains small lenses of silt and clay; mildly
          alkaline, slightly effervescent.

     The site surface profile was modified by surface disking operations that
were practiced to restore infiltration capacity.  In addition, the soil
contained more gravel and gravel lenses and generally less clay than the
typical profile.

     Other soil types (Nacimiento clay loam, Metz gravelly sandy loam, sandy
alluvial land, river wash) are located nearby and adjacent to the site.
Because soil forming processes generally do not create distinct boundaries
between soil classification units and because soil properties vary within

                                      46

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units, it was not possible  to  find  control  sites with characteristics
identical to the treatment  sites.   The  control  sites finally chosen were
reasonably similar to the treatment sites.

     The results of physical and chemical  analyses are presented separately
in the following sections.  The methods of analyses were discussed previously
in Section 6.

PHYSICAL PROPERTIES

Particle Size

     A mixture of inorganic and organic particles making up a mineral soil
can be texturally classified by determining the proportions of the three
major soil separates—sand, silt, and clay.  An exponential rise in surface
area, adsorption capacity,  swelling, plasticity, cohesion, and heat of
wetting occurs when soil particle size  decreases from sand to colloidal clay
       The effect of soil texture on the direction of change of soil physico-
       1 properties is  summarized in Figure 15.
[17].
chemical
Soil Property
Surface Area
Molecular
adsorption
CEC
Swelling
Plasticity
Heat of wetting
Water holding
capacity3
Infiltration3
Percolation*
Permeability*
External
drainage3
Aeration*
Organic matter
content3
Structure*
Bulk density*
Porosity (total)*
Soil Texture
Sand Silt Clay
Sand, loamy sand, Loam, silt loam, Sandy clay, silty
sandy loam silt, sandy clay clay, clay
loam, clay loam,
silty clay loam

••• — ^-



	 >•

- -M-
' -<
-^
-^ 	

- -->-
-< 	 • -


-V-

	 ^-
         a.  Colllgatlve properties.
         Note:  Arrow Indicates direction of magnitude Increase.
             Figure 15.  Physico-chemical  properties of soils
                as related to textural  classification [18].
                                      47

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      The particle size  distribution of  the five sampling sites is given  in
Table 13.  The  size limits of  the soil  separates  are identical  to those  used
by the U.S. Department  of Agriculture.

         TABLE 13.  PARTICLE SIZE  DISTRIBUTION IN  SOIL SAMPLE  FROM THE
                        HOLLISTER  RAPID INFILTRATION  SITE
Depth, cm
USDA textural classification3 Gravel
Control No.l
0-16
25-35
95-105
295-305
Gravelly sandy loam
Gravelly sand
Gravelly sand
Gravelly sand


6
16
30
42
Sand
Silt
Clayb


63
81
69
52
26
3
1
6
5
—
--
--
Control No. 2
0-16
25-35
95-105
295-305
Treatment
0-16
25-35
95-105
295-305
Treatment
0-16
25-35
95-105
295-305
Treatment
0-16
25-35
95-105
295-305
Gravelly sandy loam
Gravelly sand
Gravelly sand
Gravelly sand
site No.l
Gravelly loamy sand
Gravelly sand
Gravelly loamy sand
Gravelly sandy loam
site No. 2
Sandy loam
Gravelly loamy sand
Gravelly sand
Sandy loam
site No. 3
Gravelly sandy loam
Gravelly sand
Gravelly sand
Gravelly sand
4
24
56
15

20
10
38
6

2
10
30
1

6
8
29
15
66
71
43
84

67
86
54
70

64
70
69
67

64
88
70
82
23
5
1
1

13
4
8
17

28
20
1
26

26
4
1
3
7
—
—
--

--
—
—
7

6
—
—
6

4
—
•-
~~
           a.
>2 mm = gravel; 0.05-2 mm « sand; 0.002-0.05 mm » silt; <0.002 mm • clay.
           b.  Clay fractional on (by hydrometer) was performed only when the analyst had
               reason to believe that clay was present in the pan sieve  sample.  When clay
               analysis was not performed, the soil was assumed to be all silt.

           mm x 0.03937 = in.
                                         48

-------
     Control  sites are either gravelly sands  or gravelly  sandy  loams while
the treatment site textures range from gravelly sand to gravelly  sandy  loam.
In all cases, sand is more than 50% of the less than 2 mm size  fraction.
Because soils in this textural  class have large spaces between  particles,
they are expected to have a high percolation  rate,  low water holding
capacity, and high aeration capacity [18]. Field inspection of the sand
fractions from both control and treatment site locations  indicated that they
consisted largely of quartz, feldspar, and shale fragments.

Surface Area

      It has been shown that for a specialized set of conditions and spherical
particles, an estimate of soil surface area can be obtained using the
relationship [19]:


                              - = —
                              M   pr

where     A = surface area
          M = mass of soil
          p = particle density
          r = particle radius

      To calculate  rough values of  surface area per unit mass, a particle
density of 2.65  g/cm3 was  selected, while particle  radii  for each  size
fraction were calculated  from  the  average particle diameters given in Table
14.

                  TABLE 14.  SURFACE AREA  PER UNIT SOIL MASS
Size fraction
Average diameter, cm
A/M = 3/pr, cm2/g
Gravel
0.30
8
Sand
0.0102
22
Silt
0.0026
870
Clay
0.0001
22,600
                  cm x 0.3937 = in.
                  cm2/g x 70 = in.2/lb


     Estimates of  surface area distribution for a hypothetical  100 g soil
 sample  are presented  in Table 15.  While the numbers are gross  estimates
 only, they are qualitatively important because many soil-solution
 interactions, especially heavy metal  sorption, are surface  area dependent.

     Surface area  estimates  range from a low of 2,264 cm2 (351  in.2) to a
 high of 179,694 cm^  (27,853  in.2) with surface soil horizons  generally having
 the highest surface  area.   Treatment  site 1, however, has its surface area
 maximum at 300 cm  (118 in.).  (Clay analysis was not performed  on the surface
 depth fraction; a  small amount of clay would significantly  alter this
 estimate.)  A similar subsurface maximum exists for treatment site 2.
                                      49

-------
          TABLE 15.  ESTIMATED SURFACE  AREA DISTRIBUTION (cm*) FOR A
                              100  g  SAMPLE OF SOIL
Depth, cm
Control No. 1
0-16
25-35
95-105
295-305
Control No. 2
0-16
25-35
95-105
295-305
Treatment site No. 1
0-16
25-35
95-105
295-305
Gravel

48
128
240
336

32
192
448
120

160
80
304
48
Sand

1,386
1,782
1,518
1,144

1,452
1,562
946
1,848

1,474
1,892
1,188
1,540
Silt

22,620
2,610
870
5,220

20,010
4,350
870
870

11,310
3,480
6,960
14,790
Clay

113,000

158,200

158,200
Total

137,054
4,520
2,628
6,700

179,694
6,104
2,264
2,838

15,782
5,452
8,452
174,578
               Treatment site No. 2

                     0-16          16
                    25-35          80
                    95-105         240
                   295-305           8
1,408 24,360  135,600  161,384
1,540 17,400    —    19,020
1,518    870    —    2,628
1,474 22,620  135,600  159,702
Treatment site No. 3




1
0-16
25-35
95-105
295-305
cm2 = 0.155 in.2
48
64
232
120

1,408
1,936
1,540
1,804

22,620
3,480
870
2,610

90,400 114,476
— 5,480
— 2,642
— 4,534

Bulk Density

     Bulk density  measurements reflect the status  of  soil  structure, which 1n
turn affects water movement,  aeration, and porosity.   It is a measure of
total soil space not  occupied by solid matter and  is  affected by texture,
organic matter content,  aggregation, root penetration,  and compaction.  Bulk
density measurements  were performed at selected locations  throughout the
study area using the  methods  described previously.  The results are presented
in Table. 16.
                                                         3
     Average measurements ranged from 1.27 to 1.72  g/cm ,  but there were not
sufficient data to determine  if conclusive differences  existed between
control and treatment sites or among depths.
                                       50

-------
              TABLE 16.  SOIL BULK DEMSITY MEASUREMENTS (g/cm3)
                        AND CALCULATED SOIL POROSITIES

Site
Control No. 1

Control No. 2



Depth, cm
0-8
25-33
0-8
25-33
95-103
Db
Core method
1.59
1.65
1.41
1.27
1.57
Excavation D[j
method Average
1.84 1.72
— 1.65
— 1.41
— 1.27
— 1.57
%
Porosity
35
38
47
52
41
            Treatment
site No. 3
4


0-8
4-12
25-33
95-103
1.25
1.38
1.57
1.48
1.33
—
—
— ~
1.29
1.38
1.50
1.48
51
48
42
44
            g/cm3 x 27.8 = lb/in.3
          Soil porosity can be calculated from bulk density as follows:

                              %? = (1 - ^.) 100                          (2)


where %P = percent pore volume
      05 = bulk density
       P = particle density, assumed as 2.65 g/cm3

     The results of such calculations are also presented in Table 16.

     Control site 2 and treatment site 3 are similar, having porosities  in
the range 42 to 52%.  Control site 1  is slightly lower with an average
porosity of 37%.  The lower than average bulk densities of the near-surface
measurements at the treatment site may have been the result of diskina
operation.                                                       ^my

Infiltration Studies

     Water intake rates were measured for several  basins where wastewater had
been applied for 30 years.  The results of the basin flooding infiltration
studies are presented in Table 17.                               nwauiun

     The average infiltration rate for the basins  was 17.7 cm/d (0.58  ft/d)
which  s over twice the average daily application  rate of 8 cm/d (0.27 ft/d)
Basin 11  was tested without prior disking, and may have caused larger  than
normal  pore clogging and the subsequent low infiltration rate.
                                     51

-------
                          TABLE  17.   BASIN  FLOODING
                              INFILTRATION  RATES
Location
Basin 3
Basin 4
Basin 5
Basin 6
Basin 7
Basin 9
Basin 10
Basin 11
Basin 12
Basin 13
Average
Infiltration
rate, cm/d
17.7
17.7
20.4
14.6
20.7
18.3
8.8
4.6
22.0
31.7
17.7
                            cm/d x 0.3937 = in./d
     Infiltration rates are generally thought to be a function of many
factors, including initial  water content and temperature which were not
controlled in the measurement of the infiltration rate.   Nonetheless, at this
rapid infiltration site, infiltration rates were apparently related to  soil
porosity, being highest where porosity was greatest.

     Cylinder infiltrometer tests were also conducted in an infiltration
basin to determine the effect of wastewater solids and surface scarification
on infiltration rate.  Tests were conducted using clear water (SS = 0 mg/L)
and primary effluent (SS = 275 mg/L) on the soil of infiltration Basin  6,
which had not been disked since the last wastewater application.  Tests were
repeated on the same soil after disking.  The testing procedure is described
in reference [20] where buffer cylinders approximately 90 cm (3b in.)  in
diameter were continuously flooded in an attempt to approximate the true
vertical infiltration rate.  Inner test cylinders were 55 gal drum sections
46 cm (18 in.) in diameter.  A typical test cylinder is shown in Figure 16.
The infiltration rate was measured until steady state conditions were
established (generally in less than 2 hours).

     A one-way analysis of variance was performed on the infiltrometer  data
to determine if mean infiltration rates were statistically equal for the nine
treatments considered (Table 18).  The hypothesis of equality was rejected at
the a = 0.01 level.*
   a  = 0.01  level  is  the probability of rejecting a true hypothesis.
                                     52

-------
Figure 16.   Cylinder infiltrometers.




                 53

-------
     Mean infiltration rates,  standard  deviations,  and coefficients of
variation are listed in Table  18.

             TABLE 18.  CYLINDER  INFILTROMETER  IMFILTRATION RATES
Rank order
of
treatments
1
2
3
4
5b
6
7
8
9

Location
Basin 6
Basin 6
Basin 6
Basin 6
Basin 6
Control site
Basin 6
Basin 6
Control site
Soil
condition
Undisturbed
Undisturbed
Undisturbed
Undisturbed
Undisturbed
Undisturbed
Disked
Disked
Disked

Fluid
Clear water
Clear water
Clear water
Effluent
Clear water
Clear water
Clear water
Effluent
Clear water
Buffer
pond
Yes
Yes
Noa
Yes
Yes
Yes
Yes
Yes
Yes
Repli-
cates
5
5
5
6
6
2
6
6
4
Mean Infil-
tration rate,
cm/d
110
116
146
152
177
177
268
287
311
Standard
deviation,
cm/d
24
49
30
73
104
55
85
98
159
Coefficient of
variation,
X
22
42
21
48
59
31
32
34
51
  a. Cylinders were tested (1n place) after treatment No.2 without a buffer pond.

  b. Cylinders were tested (1n place) after treatment No.4 with clear water.

  cm x 0.3937 * in.
     Duncan's multiple  range  test,  a =  0.05 (probability of rejecting a true
hypothesis), indicated  significant  differences between the following
treatment  means referred  to  in  Table 18:  7-6, 7-5,  7-4, 7-3, 7-2, 9-6, 9-5,
9-3, 9-2, 9-1, 8-6, 8-5, 8-3,  8-2,  and  8-1.

     Final infiltration  rates  in all  cases were several  times the rates
determined by flooding  the  entire basin with wastewater (see Table 17).  This
phenomenon was predicted by Bouwer  [21] who suggested that a small buffer
area in relation to inner  test cylinder area may result in an overestimation
of infiltration rate by cylinder infiltrometers.  On the other hand, Burgy
and Luthin [22] reported single  cylinder infiltrometer results within 30% of
the mean infiltration rate  determined by flooding 12 m by 27 m (40 ft by 90
ft) plots.  No difference was  reported  when buffer ponds were used.  In the
second case, test cylinders were small, (15 cm [6 in.])  and buffer cylinders
were not large, (30 cm  [12  in.]), relative to inner cylinder diameters.

     The coarse texture of  the Hoi lister soil  may account for the high
measured infiltration rates.   The relatively small cylinder area as compared
to that of the infiltration basin tends to decrease the effect of gas binding
and soil pore clogging  by wastewater solids.  In addition, there was no
significant difference  between tests conducted with and without buffer
cylinders at Hoi lister.  This  suggests  that radial movement of water below
the infiltrometers was occurring.
                                     54

-------
      Infiltrometer  results did not show a significant difference (a = 0 05)
 between tests  using  clear Water and tests with wastewater.  This relation
 held for both  undisturbed or disked soil conditions.  The documented pore
 clogging effect  of wastewater solids [23] was somehow masked by infil trometer
 us s u conQi ui ons •
 •   •       u              °f diskin9 on infiltration velocity is clearly
 indicated  by the data.  Although the solids mat, which characteristically
 forms  during flooding at Hollister, had dried and formed large cracks  the
 infiltration capacity was not as great as tests conducted on disked soil.
 Also,  under similar test conditions, there was no significant difference
 between  the control site, where wastewater had never been applied,  and Basin
 6 where  wastewater has been applied for 30 years
 SOIL CHEMISTRY

 Introduction
     The soil chemistry program at the Hollister rapid infiltration site
ipin H^r  h rlyS1S °,f 23 so11 V*r™<*^'  The methods of analyse  have
been described previously.  A summary of the results is presented in Table
,1™ Pa^fierS dr< dis9ussed separately or in natural  groups.   The data  were
also plotted as a function of sampling depth.   For the  purposes of plotting
the means are bracketed by an error bar of plus or minus one standard
            To fjd.litate plotting, treatment site points were depressed 6  cm
(22 in.) from their true location.  In all  cases, squares represent control
sites, and circles represent treatment sites.                        control

rm nq°^nS?VhInLPdr?U?terS' concentration " pseudo-mi nimums"  occurred at 100
cm (39 in.) depth.  This occurred because the data were calculated  on a
 whole soil basis,  though the analytical  measurements were performed on the
 ess than 2 mm size fraction.  The larger concentrations of gravel  at 100 cm
(39 in.) caused the "whole soil"  concentrations to appear small.
NN  *nHe/Had4r iS ?dvl!?d that the sum of the exchangeable  cations Ca, Mg
Na  and K differs significantly from the cation exchange  capacity  (CEC
This was probably due to analytical  error.  Chapman  [24]  notes  that in
calcareous soils, CEC tends to be underestimated,  while exchangeable Ca is
overestimated.   The U.S.  Salinity Laboratory [12   recommends that  Ca not
                     50115 While ^ ^ ^  cautfolt
                                    55

-------
TABLE 19.  RESULTS OF SOIL CHEMICAL ANALYSES
Depth, cm
Control
No. 1
0-16
25-35
95-105
295-305
Control
No. 2
0-16
25-35
95-105
295-305
Treatment
Site No. 1
0-16
25-35
95-105
295-305
Treatment
Site NO. 2
0-16
25-35
95-105
295-305
Treatment
Site No. 3
0-16
25-35
95-105
295-305
CaCOs Conductivity,
pH equiv, % fimhos/cm


8.5
9.2
9.4
9.4


7.8
8.4
8.9
9.1


6.3
6.8
7.9
8.8


6.4
6.5
7.8
8.8


6.7
7.6
8.0
8.6


1.55
2.27
2.47
2.51


1.08
2.06
2.27
3.04


0.28
0.27
1.17
1.42


0.36
0.29
2.01
1.39


Nm
Nm
Nm
2.32


740
100
100
400


770
500
330
500


2940
2400
1800
700


2620
1280
1260
880


2470
770
500
600
Organic
matter, %


0.76
0.08
0.02
0.01


1.35
0.15
0.02
0.02


1.20
0.20
0.06
0.11


2.19
0.12
0.03
0.08


2.02
0.28
0.05
0.03
Nitrogen, PP"1
Total


560
76
64
45


840
110
34
180


1200
370
120
180


1720
350
93
190


1600
200
104
77
Organic


550
62
45
34


818
98
30
167


1100
330
99
140


1600
290
74
150


1500
174
86
65
Phosphorus
Extractable,
ppm


13
0.67
0.56
3.7


8.4
0.15
0.09
0.17


57
61
42
54


109
73
39
32


110
63
3)
21
Total -P,
ppm


710
610
390
330


790
460
360
470


1600
1200
720
960


2000
1400
790
840


2800
1400
920
850
Boron,
ppm


<0.19*
<0.17
<0.14
0.13


0.22
<0.15
<0.09
<0.17


0.66
0.36
0.18
0.38


1.08
0.35
0.17
0.47


0.70
0.34
0.16
0.20
                  CONTINUED
                      56

-------
                                 TABLE  19  (CONTINUED)
Depth, cm
Control
No. 1
0-16
25-35
95-105
295-305
Control
No. 2
0-16
25-35
95-105
295-305
Treatment
Site No. 1
0-16
25-35
95-105
295-305
Treatment
Site No. 2
0-16
25-35
95-105
295-305
Treatment
Site No. 3
0-16
25-35
95-105
295-305
CEC,
meq/100 g


7.2
2.8
1.9
2.3


8.6
3.3
1.2
2.6


4.8
4.1
3.2
6.9


11.
6.2
2.7
7.5


11.
4.2
2.0
3.2
Exchangeable cations,
meq/100 g
Na


0.93
0.11
Nm
0.12


0.41
0.07
0.02
0.05


1.2
0.77
0.39
0.91


3.4
0.59
0.22
0.74


1.2
0.48
0.26
0.14
K


0.26
0.02
Nm
0.01


0.30
0.05
NOT
Nm


0.22
0.13
0.06
0.16


0.34
0.16
0.01
0.16


0.38
0.10
0.09
0.02
Ca


5.6
3.0
1.8
1.5


5.7
1.8
0.88
3.2


0.64
Nm
2.8
4.5


1.7
0.09
2.3
6.2


0.94
0.18
0.57
2.7
Mg


1.8
1.1
1.3
1.5


1.8
1.1
0.70
7.6


3.3
2.9
2.2
4.2


7.0
4.0
1.3
4.2


6.3
2.6
1.4
1.9
Cd


0.08
0.01
0.01
0.01


0.12
0.02
0
0.01


0.14
0.07
0.02
0.03


0.24
0.05
0.01
0.01


0.18
0.04
0.01
0.01
Co


0.08
0.07
Nm
0.01


0.04
Nm
Nm
Nm


0.10
0.07 '
0.05
0.09


0.14
0.05
0.03
0.04


0.04
0.04
0.03
0.05
DTPA extractable
heavy metals, ppm
Cr


<0.08
<0.07
<0.06
<0.05


<0.08
<0.06
<0.04
<0.07


<0.06
<0.07
<0.05
<0.08


<0.08
<0.07
<0.06
<0.08


<0.08
<0.07
<0.06
<0.07
Cu


2.4
0.35
0.71
0.24


4.0
0.36
0.17
0.37


6.7
2.7
1.5
2.3


8.2
2.2
0.87
1.2


6.2
1.4
0.50
0.60
Fe


19
7.6
6.3
7.0


23
7.0
3.7
8.0


120
104
41
26


270
160
53
32


170
140
28
14
Hn


5.4
2.5
1.7
2.1


5.2
2.6
1.2
2.4


2.2
1.0
1.3
2.2


3.1
0.54
2.1
4.4


2.1
0.66
2.0
4.6
Ni


0.62
0.24
0.20
0.19


0.73
0.27
0.14
0.24


0.74
0.54
0.50
0.28


2.4
1.0
0.39
0.32


1.3
0.33
0.31
0.44
Pb


5.1
0.17
<0.14
<0.12


10.
0.36
<0.09
<0.17


3.5
0.59
0.35
0.41


5.7
0.67
0.29
0.36


6.6
0.28
0.17
0.17
Zn


0.98
0.12
0.04
0.05


1.6
0.21
0.03
0.07


11.
3.4
0.21
0.23


8.0
1.1
0.24
0.18


7.5
1.4
0.27
0.32
a.  Below detectable  limits
Note:  Nm = not measurable.
cm x 0.3937 - 1n.
                                          57

-------
Statistical Analysis of Soil Data

     Three types of statistical analyses were performed for each soil  species.
The tests and corresponding hypotheses are presented below.
    Two-way  analysis
    of  variance  (ANOVA)
    t-test (at each
    depth increment)

    Duncan's  multiple
    range test
H0:

Her

H0:
     ^controls = ^treatment site

     ^depth 1  = ydepth 2 = ydepth 3 = ydepth  4

     Depth-treatment interaction = 0

     ^controls = ^treatment site
H0:  paired treatment means are equal

H0:  paired depth means are equal
     In all cases, y equals the mean of the subscripted soil  variable.

     The tneory and assumptions underlying these statistical  analyses are
presented in several  texts [26, 27].  Briefly, it is assumed that the
hypothesis to be tested is true.  Then the consequences of this assumption
are examined in terms of a sampling distribution.  If, as determined from the
sampling distribution, observed data have a relatively high probability  of
occurring, the decision is made that the data do not contradict the
hypothesis.  On the other hand, if the probability of an observed set of data
is relatively low when the hypothesis is true, the decision is that the  data
tend to contradict the hypothesis, ana the hypothesis is rejected.

     The level of significance a, of a statistical test defines the
probability level that is to be considered too low to warrant support of the
hypothesis being tested, that is, a is the probability of rejecting a true
hypothesis.  If the probability of observed data, p, is smaller than the
level of significance a, then the data are said to contradict the hypothesis
being tested, and a decision is made to reject the hypothesis.

     The value of a was arbitrarily set at 0.10 for the two-way ANOVA, and
depth increment t-test, and ata= 0.05 for Duncan's multiple range test.
Thus when the calculated value of p was less than ot, the above hypotheses
were rejected [28].
                                     58

-------
     For the two-way analysis of variance,  measurements  for both control
treatment sites were given the following  symbolic  designations for
statistical  treatment.
                                                            and
                         D2
                         D4
                  xm

                  X112



                  X211
                  X212



                  X311
                  X312



                  X411

                  X412
X121

X122

X123

X221

X222

X223

X321

X322

X323

X421

X422

X423
where D]
      D£
      DS
      04
                       depth increment, 0-16 cm (0-6 in.)
                       depth increment, 25-35 cm (10-14 in.)
                       depth increment, 95-105 cm (37-41 in.)
                       depth increment, 295-305 cm (116-120 in )
                       control site treatment
                       application site treatment
                       k observations for DjTj
     Once coded, Biomedical  Computer Program BMDP2V,  was used  to  generate
ANOVA tables for each of the 23 soil  chemical  species [29].  With the  aid  of
such a table it was then determined if significant differences in observation
magnitude existed among depths, between treatments, and/or resulted  from a
depth-treatment interaction.
                                     59

-------
     The  physical  significance of such a statistical  treatment is illustrated
 by  three  hypothetical  situations presented below.  Two curves presented in
 each graph  represent control  and treatment sites.
                                   CONCENTRATION
                                    SITUATION A
                 CONCENTRATION
                                      CONCENTRATION
      Q


     SITUATION B

Q - CONTROL SITE

6 - TREATMENT  SITES
                                                     SITUATION C
     In Situation A, both control  and  treatment sites have concentrations
that are uniform with respect  to  depth,  but the treatment site exhibits a
statistically significant increase in  concentration relative to the control
site.  In contrast, Situation  B shows  that control  and treatment sites not
only exhibit a statistically significant difference due to wastewater
treatment, but also shows that depth introduces a significant source of
variation.  Situation C introduces a third source of variation, a depth-
treatment interaction.  In  this case,  the concentration for the treatment
site has increased at all depths  relative to the control  site.  However, a
selective enrichment is observed  where the values of depth  are low (i.e., at
the surface).  A statistical summary including  chemical  species, source of
variation (D, T, DT) calculated F-statistic, and P-value,  is presented in
Table 20.  Test statistics  were deemed significant when the condition P< ct=
0.10 was satisfied.
                                     60

-------
         TABLE 20.  ANALYSIS OF VARIANCE FOR SELECTED SOIL PARAMETERS
Species
CEC


Na


K


Ca


Mg


Cd


Co


Cr


Cu


Fe


Mn


Ni


Source
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
F
10.02
4.70
0:46
4.23
6.51
0.73
25.93
9.09
0.53
6.10
4.99
10.68
2.89
3.20
1.89
33.98
10.43
2.97
3.39
7.04
0.31
6.35
1.99
1.54
39.94
28.56
4.69
7.14
30.47
5.30
14.17
5.80
11.16
4.66
4.88
0.73
P
0.001
0.051
0.718
0.029
0.025
0.552
0.000
0.011
0.669
0.009
0.045
0.001
0.079
0.099
0.185
0.000
0.007
0.074
0.054
0.021
0.817
0.008
0.184
0.253
0.000
0.000
0.022
0.005
0.000
0.015
0.000
0.033
0.001
0.022
0.047
0.552
Species
Pb


Zn


PH


CaCO-
J

Conduc-
tivity

Organic
Matter

Total -N


Organic-
N

Extrac-
table-P

Total -P


Boron





Source
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT
D
T
DT



F
29.22
0.68
1.33
35.61
38.55
19.58
20.60
66.33
3.31
7.91
35.54
0.86
5.97
27.16
2.63
35.11
4.03
2.29
57.82
18.62
7.89
66.70
17.95
8.24
5.31
66.46
2.91
9.30
37.63
2.86
9.40
26.92
5.56



P
0.000
0.427
0.310
0.000
0.000
0.000
0.000
0.000
0.057
0.004
0.000
0.489
0.010
0.000
0.098
0.000
0.068
0.130
0.000
0.001
0.004
0.000
0.001
0.003
0.015
0.000
0.078
0.002
0.000
0.080
0.002
0.000
0.013



     In addition to the two-way ANOVA, a t-test for each depth increment and
a Duncan s multiple range test were performed.  These analyses helped isolate
the  location(s) of heterogeneity" within the soil  profile.  The analyses
were performed using a Hewlett-Packard combination desktop calculator-
plotter.  Sample output is given in Table 21.  Outputs for all soil  data are
shown in Appendix B.
                                     61

-------
                      TABLE 21.   SAMPLE OUTPUT FOR STATISTICAL
                               ANALYSIS OF  SOIL DATA
                                      pH, Units


Depth, cm
1. 0-16
2. 25-35
3. 95-105
4. 295-305


Control 1
(1)
8.500
9.200
9.400
9.400


Control 2
(2)
7.800
8.400
8.900
9.100

Treatment
site 1
(3)
6.300
6.800
7.900
8.800

Treatment
site 2
(4)
6.400
6.500
7.800
8.800

Treatment
site 3
(5)
6.700
7.600
8.000
8.600


Mean
(6)
7.140
7.700
8.400
8.940
8.045

RSDa
(7)
13.528
14.520
8.460
3.502

       5.  TOTAL
       6.   MEAN
       7.   RSDa
           0-16
           25-35
          95-105
         295-305
36.500
 9.125
 4.682
34.200
 8.550
 6.786
 Control Average

     8.150
     8.800
     9.150
     9.250
        GROUPS OF NONHETEROGENEOUS MEANS"
        DUNCAN'S MULTIPLE RANGE TEST.
                8.940
                8.400
                7.700
                7.140
     8.400
29.800
 7.450
15.047
29.500
 7.375
84.333
30.900
 7.725
55.285
               Treatment Site Average

                      6.467
                      6.967
                      7.900
                      8.733
160.900
                                                                    "t"
                            -5.545880
                            -3.537971
                            -6.228411
                            -3.661871
                9.125
                8.550
                7.725
     8.550

     7.450
    7.375
        a.  Relative Standard Deviation.
        b.  Numbers on same horizontal line are nonheterogenous.
           Mean column (6); second set refers to Mean line 6.
                                     First set of numbers refers to
      For an a = 0.10, the  computed,  absolute value of t must exceed 2.353
before the hypothesis of equality of means can  be rejected.   So for the  case
presented, treatment site  and control  site values of pH are  significantly
different at  all  depth  increments.

pH  and Calcium Carbonate

      The pH of the soil-water system is  critical, since it serves as a
"master variable"  controlling the solubility of many elements and influences
microbial  activity.   It is  important to  know both the initial  pH of a soil
system and the expected changes in pH  as the system is manipulated over  time.

      In a "natural  system,"  soil pH  change is affected by biological
activity,  organic  matter, alkaline-earth carbonates, and by  hydrous oxides  of
                                         62

-------
 aluminum  and  iron.  For  an  arid-zone, nonsaline  soil,  such  as  the Metz,
 calcium carbonate undoubtedly exhibits a major pH control.

     The  addition of wastewater  to  the treatment site  has significantly
 decreased soil  pH (Table 20).  This pH reduction was apparent  at all  depths
 (Table 21).   The pH differences  between control  and treatment  sites ranged
 from 0.5  to 1.6 units depending  on  the depth below the surface.  The  highest
 average pH values were found at  depths of  300 cm (118  in.), while mildly
 acidic conditions (pH 6.5)  were  found in samples from  the surface of  the
 treatment sites (Figure  17).

     Simultaneously, calcium carbonate concentrations  have  been depleted at
 the treatment site.  The buffering  capacity of the soil was thereby
 diminished, permitting a pH reduction.  The effect was most pronounced in the
 surface 30 cm (12 in.) where the difference was  statistically  significant.

     The  ultimate fate of Ca, dissolved from calcium carbonate, is uncertain
 as there  had  been no noticeable  accumulation of  CaCO,  at depths through 300
 cm (Figure 18).  Although there was a slight increase  in exchangeable Ca at
 300 cm, it was  not statistically significant.  Therefore, it is possible that
 Ca was passing  through to the underlying groundwater.
                   PH

            6. 00  7. 00  8.00  9. 00
      so
     I 00
     I 50
     200
     250
     300
                  1
                    ,„,
k   4-
                                           .   150
                                              200
                                              250
                                              300
                                                   0-5 1.0 1.5 2.0 2.5 3.0
                                                    1 - ' - I  IT-, I - 1 - 1 - 1 - 1 - 1 - 1 - 1
          LE8END

          0= CONTROL SITES

          0= TREATMENT  SITES
Figure 17.  Vertical distribution
            of soil pH.
                     Figure 18.  Vertical distribution
                         of soil calcium carbonate.
                                      63

-------
     Interestingly, the average surface soil pH at the treatment site (pH =
6.5) was less than the average pH of the applied wastewater (pH = 7.3).   This
suggests an additional mechanism(s) for pH reduction.  One possibility is the
nitrification of wastewater ammonium to nitrate according to the following
reaction:

                 NHj + 202 = N03" + H20 +  2H+                             (3)


     The creation of 2 moles of hydrogen for every mole of NH4  oxidized
would  further reduce the pH of the soil system.  In addition, it can be
postulated that the species CaN03 would form, accelerating the leaching of Ca
from the soil profile.

     It  should be noted as well that the pH at the control site was often in
excess of that which would be predicted by equilibrating CaCO- with water (pH
= 8.2).  Therefore, the presence of a more soluble carbonate or bicarbonate
species  (NaC03, MgHCOo) is suggested.

Conductivity of the Saturated Extract

     Conductivity serves as a quick estimate of the amount of soluble salts
in  the soil system.  The measured value is a function of both temperature and
soil water content at the time of measurement.  The USDA salinity laboratory
has published several graphs and tables that allow conversion of conductivity
measurements to soluble salt concentration.  Input of salts at a rate greater
than that removed by leaching will increase the measured conductivity.  It is
a valuable parameter for monitoring soil salinity and predicting plant
toxicity.

     A plot of average conductivity versus depth is presented in Figure 19.
Both treatment and control sites show the same general trend, that is,
highest extract conductivity at the surface which decreases with depth.
Treatment site values are significantly higher than the controls, rising to
nearly 2,700 ^mhos/cm at the surface (see Table 20), reflecting the input of
soluble salts in the wastewater.  The interaction between depth and treatment
is also significant (p = 0.098), as exhibited by the larger differences
between surface conductivity values and those at depth.

     The lack of significant difference between treatments at 30 and 100 cm
(12 and 39 in.)  resulted from the large standard deviations at these depths.
Though not statistically significant,  salinity increases may be occurring at
these depths as well.  At this level  of soil  extract conductivity,  these
soils could successfully grow any crop although the most salt sensitive  crops
might suffer some yield diminution.
                                     64

-------
                         ELECTRICAL CONDUCTIVITY, mmhos/cm
                                             LEGEND

                                        O CONTROL SITES

                                        O TREATMENT  SITES
                     Figure 19.   Vertical  distribution
                   of saturated  paste,  soil  conductivity,
Organic Matter

     Soil organic matter influences soil  water holding capacity, the
stability of soil structure, pH, the exchange capacity, the retention of
trace metals by chelation, and the cycling of carbon, nitrogen, and
phosphorus [17, 30].  It has a much higher gravimetric cation exchange
capacity than any clay mineral and has a surface area comparable to that of
expandable clays [18].  Its exchange capacity is highly pH dependent, being
very low at pH values less than 5, but rising to over 300 meq/lOOg at pH
values of 8.5.  Of some interest is the fact that organic matter tends to
counteract the unfavorable effects of high exchangeable sodium percentage on
soils by stabilizing aggregates of clay minerals, thus diminishing dispersion
and pore clogging.  It serves as an energy material for microorganisms which
in turn form humates, fulvates, and other polymers which form and stabilize
aggregates.

     The balance between input and decay of organic matter is modified by
such factors as composition of the input material, type of microorganisms
present, temperature, pH, availability of moisture, and the oxygen content in
the immediate environment [17].

     An analysis-of-variance  (Table 20) of the Hoi lister soil data revealed a
significant increase in soil  organic matter resulting from wastewater
application.  Control site values range from 1.35% at the surface to 0.01% at
300 cm (Figure 20).  Treatment site values range from 2.19% to 0.03% for the
same depth increments.
                                      65

-------
                                   ORGANIC MATTER,  »

                                  O.S   1.0  1.5  2.0
                           100
                           1 SO
                           200
                           290
                           300
                                             LEGEND

                                          CONTROL SITES

                                        O TREATMENT SITES
                        Figure 20.  Vertical distribution
                             of soil organic matter.
      In  contrast  to  the  analysis-of-variance, the  t-statistic  revealed no
 contrasts  between control  and  treatment  site locations at any  depth
 increment.  This  apparent  discrepancy may have  resulted  from the smaller
 number of  degrees of freedom used  in the t-test or the assumptions implicit
 in  the analysis-of-variance may have been violated.

      Because of the  above  anomalies, the buildup of organic matter, if any,
 must  be  considered small.  This would suggest that the soil microbial
 population existed in sufficient numbers to bio-oxidize most of the incoming
 organic  matter.   It  should be  noted, as  well, that the BOD of  the shallow
 treatment  site wells was not significantly different (t-test,a = 0.10) than
 the offsite control  wells, further suggesting that degradation of bio-
 oxidizable material  was occurring.  The  fact that  COD and TOC values showed
 significant increases relative to the control wells suggests that some
 "refractory" organics were passing through the  soil profile.

 Nitrogen

     The nitrogen cycle in soils is quite complex.  When presented in a
grossly simplified manner, it can be viewed as  follows:  plants acquire
nitrogen as ammonium or nitrate ions and form organic nitrogen; after death,
the organic nitrogen is mineralized into ammonium  ion which in turn may be
acquired by living plants or nitrified to nitrate.  Nitrate may be acquired
by plants or denitrified to either nitrous oxide or nitrogen gases.
                                     66

-------
     Nitrogen accumulation can be expected to occur when organic forms of
nitrogen are introduced into the soil system, or when the dominant  form of
nitrogen is the ammonium cation [18].  In contrast, nitrogen losses are
expected when nitrogen is in a mobile anionic form (N0o~, NO ~) or when it  is
converted to a gaseous species (N2, N20).  The former Toss i$ by leaching,
while the latter loss is by atmospherTc escape and leaching [23].

     The rates of nitrogen conversion, and therefore rates of accumulation
and losses, are influenced by pH, temperature, aeration, moisture,
availability of a carbon source, and the presence of the appropriate
microorganisms.  The rate and magnitude of nitrate loss to the underlying
grounawater is of some importance because it has been one of several
associated causes of methemoglobinemia in infants.  The USPHS has recommended
that domestic water supplies contain less than 10 ppm nitrate-N.

     Significant concentration increases resulting from wastewater  treatment
were observed at the Hoi lister site for the two nitrogen species measured--
total  and organic-N.  Differences were significant for the first three depth
increments, but nonsignificant at 300 cm (118 in.).  The results are  plotted
in Figures 21 and 22, respectively.
                 TOTAL-N. ppm
                                                        ORGANIC-N. ppm
         so
        100
        130
        zoo
        250
        300
               400   800   1200  1800
               -I	r-=-l	1	1	1	H-
                                                50
                                                100
                                                ISO
                                             ui  200
                                                250
                                                300
                                                      400  BOO  1200  1600
                                                      -I	r-^r-l	'	1	i	1	
                                                       LEGEND

                                                    O  CONTROL  SITES

                                                    O  TREATMENT SITES
      Figure 21.  Vertical
      distribution of soil
      total-nitrogen.
Figure 22.  Vertical
distribution of soil
organic-nitrogen.
                                     67

-------
     Total-N and organic-N were seen to be closely related in both trend with
depth and magnitude.  The highest concentrations of total-N occurred at the
surface where values of nearly 1500 and 700 ppm were observed for treatment
and control sites respectively.  The values decreased by factors of one-fifth
and one-tenth at 300 cm depth.  Organic-N accounted for 80% or more of the
total-N throughout the soil profile, though it accounted for only 40% of the
total-N in the wastewater.  This suggests that organic-N was being
preferentially bound by the soil.

     A comparision of total-N input with the mass of total soil  nitrogen
currently in excess of background levels (mass balance) revealed that the
surface 300 cm of soil can account for only 2% of the total-N applied over
the 30 year period.  This implies that much of the nitrogen had been
converted to mobile forms and no longer exists within the sampled soil
profile.  Conversion to N? or N20 would result in atmospheric losses, while
conversion to NO," would result in losses to the underlying groundwater.
Evidence for botn processes is presented in the following paragraphs.

     Nitrate-N levels in the shallow groundwater were higher than input
levels (Section 9, Table 28 ) and suggest that some nitrification was
occurring.  However, the high background nitrate-N levels in the offsite
control wells, especially at 9C, make this conclusion tentative.

     The case for denitrification is presented as follows:

     Organic-N is generally coverted to N03-N without much change in absolute
concentration. If nitrification and subsequent loss to the underlying
groundwater were occurring, total-N levels in the shallow aquifer would be
expected to approach input total-N levels.  Examination of the groundwater
and effluent data (Table 28) reveals that input levels of total-N are
significantly greater (t-test, p = 0.00) than total-N levels in the shallow
groundwater.  This suggests that denitrification and subsequent atmospheric
nitrogen loss was occurring.  In addition, the necessary pH, carbon source,
and anaerobic conditions (when flooded) for such a conversion exist at the
treatment site.  Further evidence for the reducing conditions necessary for
denitri
reduction
      ification are given in the heavy metals  section  where  evidence for
      ion of Mn09 to Mn+2 is presented.

Phosphorus
     Soil phosphorus is known to occur both in organic and inorganic forms.
The chemistry and thermodynamic relationships of the organic forms,  however,
are less well understood.

     In general, the major controls exerted by the soil  system on Inorganic
phosphorus availability are adsorption onto clay minerals and hydrous oxides
of iron, aluminum, and manganese as well  as the formation of
cryptocrystalline precipitates with calcium, iron, and aluminum.   Organic
forms are thought to be adsorbed in a manner similar to inorganic forms  [18].
Because the removal  mechanisms are still  not fully understood, the term
sorption is increasingly used to describe any loss of soluble phosphorus from
the system [17, 23,  31].  The extent of sorption is modified by soil  type,


                                     68

-------
particle  size   pH   redox  potential,  temperature,  organic matter content, and
reaction  time  LloJ.

      Classical  adsorption isotherm expressions have been used to describe
phosphorus  sorption.   The two most common are the Langmuir and Freundlich
expressions; though  the slow mineralization of phosphorus in organic matter
as well as  the  slow  migration of  adsorbed P to interfacial  precipitation
^^r'oon1'65^ imP°sslble *> determine the precise sorption capacity of a
soil  [32].  Theoretical and empirical  equations have been used to describe
the kinetics of phosphorus uptake by soils [31].

      At the Hoi lister  rapid infiltration site, significant differences
between control  and  treatment sites  were observed (p = 0.002) for both total
and bicarbonate extractable phosphorus at every depth increment   The
9\gn*!S$inCCnSlh%dJP?h-^eat"!ent interaction is  easily observed in Figures
23 and 24.  Both total and extractable P show a greater accumulation in the
surface 100 cm  (39 in.) of the  soil  profile than  shown at depth.  At the
surface,  treatment site total-P increased from 750 to 2,100 pom while
extractable-P increased from 9  to 92 ppm.
                 TOTAL-P. ppm

              500  1000 1900  2000 2100
        SO
       100
       I 30
       200
       230
      300
                        -i	h
                                                  90
                                                 100
                                                 190
                                                 200
                                                290
                                                300
              BICARBONATE-P. ppn

              70  *o   go  10  100
              *  i  I	.—t	r—I	,—«—.
                 LEGEND

            D  CONTROL SITES

            O  TREATMENT SITES
Figure 23.   Vertical distribution of
            soil total phosphorus.
Figure 24.  Vertical distribution
            of soil bicarbonate
            extractable phosphorus,
                                     69

-------
     The fact that extractable-P was so much higher at all  sampling  depths  in
the treatment sites not only suggests that biological  availability was  high,
but also that soil solution P may have been passing through the soil  profile.
The latter is verified by noting that shallow groundwater concentrations  of
total  P were dramatically larger (8 and 10 mg/L) than baseline concentrations
of about 0.1 mg/L.  In addition, shallow well levels of total-P were
approaching input levels.

     Mass balance calculations, performed by comparing total-P input over the
30 year period to measured soil sorbed phosphorus, revealed that only 30% of
the total P applied had been retained within the upper 300 cm (118  in.) of
soil.   Again, this suggests significant transport of P to the groundwater
bel ow.

     In addition to the field studies, phosphorus isotherms for both surface
control and surface treatment sites were generated using the nondestructive
technique of Enfield and Bledsoe [10].  Freundlich isotherms were used  to
describe the shape of the sorption curves.  In Table 22, the values  K,  n, and
multiple -r2 terms are presented for the following equation:

                                   S = KCn                               (4)

where  S = sorbed P, ppm
       C = equilibrium P concentration, mg/L
     K,n = constants related to the energy of sorption


                TABLE 22.  CALCULATED FREUNDLICH COEFFICIENTS
                      FROM PHOSPHORUS SORPTION ISOTHERMS
Location
Control, 0-16 cm




Treatment site,
0-16 cm

Time,
h
1
3
53
120
288
1
53
288
K
6.82
14.7
22.2
28.6
33.1
3.64
7.25
16.0
n
0.661
0.576
0.459
0.478
0.470
G.868
0.729
0.579
r2
0.99
0.99
0.97
0.93
0.98
0.92
0.91
0.85
                    cm x 0.3937 = in.


     The corresponding sorption curves are plotted in Figures 25 and 26.   The
extent of sorption is a function of time and is observed to increase as the
equilibration time increases.  Of particular interest, however, is the extent
of sorption at the treatment site.  After 288 hours, an equilibrium
concentration of 20 mg/L will yield 92 ppm sorbed P at the treatment site,
which compares with 135 ppm at the control site for an equivalent
concentration.  Thus, even after 30 years of wastewater application, the
surface soil is retaining 68% of its experimentally determined sorption
capacity.

                                     70

-------
    411


    300



    111
    111
     • 9
     II
     II

     • I

     SI
            3 h
                 l_h-
o.i i
 1.1
        LEgENC

        •  IT

        •  3 h

        •  93 h

        A  120 h

        •  211 h
  2   3  4  I I 7 I III      21  31  41 91


EtUILIRRlUN CONCENTRATION, C, • I/L


          NOTE:

            PREDICTED 30  yr ISOTHERH
            ir ENFIELOS APPROACH.
Figure  25.   Logarithmic plot of Freundlich
regression  equation to the  experimental
data  for sorbed  phosphorus  as a function
of time and equilibrium concentration.
Control  site composite, 0-16 cm depth.
                                                                 100
                                                                 • •
                                                                 10
                                                                 70
                                                                 10
                                                                     211 h
                                                                     53 h
                                                                      1  h
                                                                         I    liitltllt     II   tl 40 50


                                                                       EQUILIIIIUI CONCENIItTION,  f,mt/l


                                                                    LE8END
                                                             •  1 h

                                                             A  53 h
                                                       Figure 26.  Logarithmic plot of Freundlich
                                                       regression equation to the  experimental
                                                       data  for sorbed  phosphorus  as a function
                                                       of time and equilibrium concentration.
                                                       Treatment site composite, 0-16 cm depth.

-------
     Sawhney and Hill [33] found equivalent behavior for Connecticut  soils
and suggested that alternating periods of wetting and drying may  bring  fresh
mineral surfaces into equilibrium with the soil  solution,  creating  new  sites
for P sorption.  Enfield [32] notes that there is a balance between sorption
and conversion of adsorbed P to more insoluble forms.  He  suggests  that
sorption is initially rapid onto mineral  surfaces, but as  time  increases,
reactions occur which utilize adsorbed orthophosphate to form phosphate
minerals that have solubilities that are somewhat less than the adsorbed
forms.  This regenerates some sites for adsorption.  Therefore, short-term
laboratory P isotherm studies represent minimum estimates  of field  P
sorption.

     Tofflemire [34] approached the problem of relating laboratory  P
isotherms to field studies in an empirical manner.  He noted that at  Lake
George, New York, a "mineralization factor" of 6 could be  used  to relate
actual field P removal  after 36 years of wastewater application to  that
predicted by a 120 hour isotherm.  Further, he indicated that a factor  of at
least 2 would be appropriate for most rapid infiltration systems.  Using the
Hoi lister soils data and Tofflemire1 s approach,  a "mineralization factor" of
15 is obtained (1,367/91 = 15, where 91 ppm is the amount of P  sorbed after
120 hours at 12 mg/L equilibrium concentration [the average total -P input
level] and 1,367 ppm is the average difference between control  and  treatment
site total-P concentrations).

     Enfield [31] and Enfield, et al . [32] took a more quantitative approach
to relating laboratory studies to actual  field P removal.   They noted that P
removal is a function of solution P concentration in the surrounding  solution
(C), and the amount of P already sorbed by the soil (S).  Three functions are
presented below:


                      Model              Model integrated
                 differential  form      form  (S - 0, at  t -  0)


               1.   ff-a(KC-S)      S=K

               2.   ff- 30.cn.  s)     S - .C" -
3.      -aCbsd       S =
                                               (1 - d)at]
     If values of 3.S/3t, C, and S are known (from isothermal plots) the
constants a, K, 3, m, n, a, b, and d can be evaluated using multiple
regression techniques.  The results of such an exercise are presented in
Table 23, for t in hours, C in mg/L, and S in ppm.  Because concentrations
greater than 20 mg/L are generally not encountered in domestic wastewater,
only points below this equilibrium concentration were included when
evaluating these coefficients.
                                     72

-------
                        TABLE 23.  EVALUATION OF THREE
                      DYNAMIC PHOSPHORUS SORPTION MODELS
Mode]
1

2


3


Coefficient
a
K
0
m
n
a
b
d
Value
0.369
6.64
0.45
22.47
0.53
4490
2.24
-3.48
r2
0.75




0.77


     Plots of dynamically predicted versus equilibrium observed values of
      are presented in Figures 27 through 29, respectively.   Models 1  and 2
are adequate when values of as/3t are greater than 5 ppm/h (i.e., when
equilibration times are short), but fail  when as/3t is small.  Model  3
appears to be the least satisfactory model, and is inadequate throughout the
range of 3S/3t.  Order of magnitude differences are observed.

     As with most models, they should not be extrapolated beyond the
experimental equilibrium times.  Models 1  and 2 approach KC  and mCh
respectively, as t increases, while S approaches infinity as t increases in
Model 3.  Therefore  in their present form, these models cannot be used as a
predictive tool for land treatment operations.

     In an effort to overcome this problem, Enfield [32] introduced a
graphical  interpolation technique.  Underlying this empirical technique is
the assumption that phosphorus removal  is a function of C, S, and t.

     Using Enfield1s approach, a predicted 30 year isotherm  was constructed
and graphed in Figure 25 (previously mentioned).  As is observed  Ehe amount
of sorption is shown to increase markedly over that recorded at 120 hoSrs
Previously  it was shown that the actual  amount of P removal  at the Hollister
site was 1  367 pprn.   This compares to a predicted value of 325 ppm that would
be expected to be removed at an equilibrium concentration of 12 Sg/L    Hence
this method underestimates the soil's removal  capacity by a  factor of 4
More research is necessary to relate laboratory P sorption to field studies
Factors such as PH and Ca concentration are factors that should be accounted
for in any future models.  Both the models of Tofflemire and EnfilldSave
been shown to be conservative, and therefore still  useful  for design
D u i \J\J jC o •
purposes
                                     73

-------
                                                             IODEL 2
                      IODEL  1
          49.0-

       JC

       1  "-^
       D.
          0.00


         -7.50 a
                                          5. 39
                                          O.


                                         J »«
                                                IS>
                                                fO J 4
                                                  12 ^
             «  9-10192029303940

                  3S/3t, ppm/hr
                                45 50
                                            i  9  I o 19 ;g » 11 11 « is tg

                                                  3S/3t, ppm/hr
Figure 27.   Dynamically  predicted values   Figure 28.  Dynamically predicted
versus equilibrium observed values of      values versus equilibrium observed
sorption  rates (aS/9t).   Model 1.           values of sorption rates (3S/9t).
                                              Model  2.
                    MODEL  3
      §. i. 40
      O-
 .700



 .350



 0.00



- . 3 50



-.710



-1.15 -
                                           & DYNAMICALLY MEOICTED

                                           • EOVILIBRIUM memo

                                           NOTE: I0«£ »OINTt tVITTID F0» CLARITY
                3S/3t, ppm/hr
  Figure 29.  Dynamically predicted values
  versus equilibrium observed  values of
  log sorption  rates (3S/8t).   Model 3
                                        74

-------
 Boron
      Boron  is  an  agriculturally important species because small  amounts are
 essential  for  plant growth, while even a slight excess can be toxic to some
 plants L36J.   Boron concentrations in irrigation waters in excess of 0.5 mq/L
 are considered undesirable for boron sensitive crops such as citrus  stone
 and pome fruit trees as well as grapes [36].                       '      '

      The increased use of boron compounds in certain household laundry
 products has tended to aggravate the problem [37].  Bingham reports that in
 the Santa Ana  River system in California as much as 50% of the 0 75 to 1  50
 mg/L boron  in  the sewage effluent comes from household sources [36].  Purves
 and Mackenzie  also showed that boron in sludges and municipal  waters is
 largely in  a water soluble form [38].

      It is  generally assumed that the orthoboric acid species exists in
 nature.  Since the pK of the first dissociation constant is 9.2  the
 undissociated  form, H3B03, is thought to be the dominant species in natural
 systems L39J.

      Many soils are capable of immobilizing boron.  Boron adsorption occurs
 mainly onto the mineral fraction of the soil, though organo-boron
 combinations do occur [17].  Boron can be adsorbed onto iron and aluminum
 hydroxy  compounds present as coatings on, or associated with,  clay minerals
 [40,  41]; onto  iron or aluminum oxides in the soil [42];  and onto micaceous
 clays  although  all clay minerals show some capacity for boron retention [431
 Rhoades, et a! . [44] concluded that in arid soils boron adsorption is
 associated with magnesium hydroxy clusters or coatings on the weatherinq
 surfaces of ferromagnesium minerals such as olivine, enstatite,  oiopside
 auguite, tremolite, and hornblende, as well  as the micaceous layer silicates.

     Boron adsorption occurs over a wide range of PH.   Such results suggest
 that boron is adsorbed as boric acid under acid conditions and borate ion as
 the  PH approaches 9.0 [41].   Boron adsorption also takes  place independently
 of concomitant adsorption of other anions.   Studies by Schalscha  et al   F451
 revealed no effect from the simultaneous adsorption of sul fate oJ ffiofftate
 on boron adsorption.   Griffin and Burau [46] found evidence for  th?ee
                                                       tnterfacially adsorbed
            depth-treatment interaction was observed  as well, caused by the
              an in the control  soil  is  nearly  unifonn with depth, havinq a
          .    5f °'16 ppm'  the ° to  16 cm  <°  to 6  in.) treatment  site sSoles
had approximately four times more boron  than  the control  samples  S1^esamples
differences  were much less  at 30 and 100 cm,  but are  statistically
significant.  Boron may be  passing through  to underground waters  thouah a
significant  increase in boron was not observed  at  300 cm.      s' ™OU9h a
                                    75

-------
                                  BORON, Ppm
                           zoo •
                           250 .
                           300
                                             LEGEND

                                         D CONTROL SITES

                                         O TREATMENT  SITES
                   Figure 30.  Vertical distribution of
                               soil boron.
CEC and Exchangeable Cations

     Electrostatic charges at soil surfaces arise from atom-proxying in
aluminosilicate minerals, proton-hydroxide reactions at the aqueous-solid
interface, and specific adsorption of weak acids or bases [47,48].  These
negative charges create electromagnetic force fields which attract cations
and repel anions. The exchanged ions near the charged surfaces are in dynamic
equilibrium with the cations of dissolved salts in an outer solution.  If the
composition of the salt solution changes, it is reflected by a corresponding
change of composition in the inner exchange phase.

     The soil cation exchange capacity (CEC) is a property of paramount
importance for it is associated with nutrient availability, soil
permeability, and the ability of the soil to desorb cations from a wastewater
influent.

     Numerous equations have been developed for describing exchange reactions
[48].  Most appear to have been derived from classical mass-action
expressions or Donnan theory [49].  In general, the final form of these
equations takes the form:
AX + B+ - BX + A*
                                     for the reaction
                                                                         (5)
where the  quantities  in  brackets represent activities of solution Ions and
the  quantities  in  parentheses are exchanger concentrations [50].

                                     76

-------
      The selectivity coefficient, KAB, is generally  not constant over a wide
 range of exchange compositions.  Therefore, models using single-valued
 coefficients can only be used semi quantitatively  over limited concentration
 ranges.  This is largely due to the existence  of  nonequivalent bonding sites
 on the soil surface [47, 50].  More quantitative  approaches treat the
 coefficient as a function of exchange composition.

      The actual  soil CEC is a function of pH,  presence of organic matter
 surface area, type of clay mineral, and the analytical  method used [24] '
 Generally, that portion of the soil that hydroxylates or deprotonates easily
 as the pH is increased (amorphous hydrous oxides  and organic colloids) will
 show a corresponding CEC increase.  That fraction of the CEC due to atomic
 proxying will remain relatively unaffected by  changes in pH.  Soil surface
 area can be correlated with the CEC.                                »un Mg+2> K+> Na+

 when the ions are of comparable concentrations.

      Empirical relationships exist [12] that relate  solution equilibrium
 concentrations of soluble cations to the concentration of exchangeable
 cations.   The most widely used are:                          manseauie


                       ESR «  -0.0126 + 0.01475  (SAR)                              ,&\
                       EPR =  0.0360 + 0.1051 (PAR)                                tj\

               where    ESR =  exchangeable sodium ratio


                          *  exchangeable sodium
                            CEC - exchangeable sodium

                       EPR *  exchangeable potassium ratio

                         ••  exchangeable potassium
                            ttt - exchangeaoie potassium
where  Na+, K+, Mg+2, Ca+2  refer to concentrations in meq/L.
                                      77

-------
     The older SAR-ESR relationships have been modified to include  additional
water quality parameters.  Certain combinations of water soluble salts will
result in the deposition or dissolution of calcium carbonate,  thereby
affecting the SAR.  Rhoades [51] presents the equation:

     ESR      =  SAR        [1 + (8.4 - pHc)]2                            (8)
     soil        irrigation
     surface     water

     Tables for calculating pHc are presented in Ayers and Westcot  [52].

     In arid fine grained soils, an ESR greater than 0.15 is generally
considered serious, though susceptibility to permeability degradation  is  a
function of mineralogy, cementing materials, and other factors.   Highly
hydrated Na  replaces less hydrated Ca+^  and Mg+2, resulting in  increased
dispersion of clay.  This in turn adversely affects soil  penneability.

     Soils in the application site showed a significant increase in  CEC with
respect to depth and treatment.  However, no depth-treatment interaction  was
observed.  Because of the smaller number of degrees of freedom,  the  more
specific t-statistic did not show significant CEC increases due  to  treatment
at any depth (Figure 31).  Duncan's range test indicated that CEC increases
were significant only for treatment Site 2 over control Sites 1  and  2.

     Control site CEC value averages ranged from 1.5 to 7.9 meq/100  g, while
treatment site values ranged from 2.6 to 8.9 meq/100 g.  Highest values were
at the surface with lower values at depth.  In spite of the pH decrease
resulting from wastewater application, CEC increased.  It is therefore
presumed that the resulting loss of any pH dependent charge was  more than
offset by the influx of organic matter and mineral clays in the  wastewater.
As a general rule, a 1% increase in soil  organic matter or clay  content can
result in a CEC increase of 3 or 0.8 meq/100 g, respectively.

     Three exchangeable cations (Na, K, and Mg) showed significant  increases
in concentration after wastewater treatment.  Significant differences
occurred for Mg at the surface and at 30 cm (1 ft), for Na at 30 cm (1  ft)
and 100 cm (39 in.), and for K at 30 cm only.  No significant depth-treatment
interactions were observed for any of these cations (Figures 32  through 34).
This is especially apparent for Na and K whose increases appeared nearly
uniform throughout the soil profile.  The effect of wastewater addition on Ca
(Figure 35) is confounded by the possibility of analytical error and chemical
interactions and is discussed subsequently.  The interpretation  of  Mg
behavior is complicated by the inclusion of a 300 cm (10 ft) control value
that appears to be anomolous (7.6 meq/100 g).  Laboratory contamination
appears to be the cause of this high value.

     As was described previously, SAR and PAR are often used to  predict ESR
and EPR [48, 51].  This exercise was performed for Hollister soils.  The
results are presented in Table 24.
                                    78

-------
     so
    100
    ISO
UJ   200
    230
    300
           CEC , maq/IOOg

          2.0  4.0  S.O  1.0 10.0

        -,—I—,—I—,—I—i A  i—I
         SODIUM, meq/IOOg
Figure 31.   Vertical
distribution of cation
exchange capacity.
                                  200
                                  250
                                  300
         POTASSIUM , meq/IOOg

           01    0.2    0.3
Figure 32.   Vertical
distribution of
exchangeable sodium.
                                                         100
                                                         1 50
                                                        200
                                                        250
                                                        300
Figure 33.   Vertical
distribution of
exchangeable potassium.
          MAGNESIUM,  neq/IOOg
                                                             CALCIUM, miq/IOOg
         1.02.0    4.05.08.0
      Figure 34.  Vertical
      distribution of
      exchangeable magnesium.
                                                      50
                                                     100
                                                     I 50
                                                     200
                                                     250
                                                     300
                                                            1.0  2.0  3.0 4.0  5.0
                                   LEGEND

                             O  CONTROL SITES

                             O  TREATMENT SITES
                                                    Figure 35.   Vertical
                                                    distribution of
                                                    exchangeable calcium
                                        79

-------
           TABLE 24.  MEASURED AND CALCULATED EXCHANGE RATIOS FOR
                SOILS AT THE HOLLISTER RAPID INFILTRATION SITE
                            ESR 	^     -«	 EPR
                 -<— calculated   >  measured     -^— calculated —^- measured


        Location
Treatment site
Control site
0.15a
0.07b
0.30
0.10
0.02b
0.04
0.04
       a. Reference [51]

       b. Reference [12]
     While the results for predicted and calculated EPR are in close
agreement, the data in Table 24 indicate that more exchangeable Na is present
than can be predicted using either of the previously discussed functional
relationships.  One explanation is that the salts of Na, Ca, and Mg have been
concentrated by evaporation before transport down the profile.  As the
absolute solution concentrations of Na, Ca, and Mg increase, the colloid
preference for Na would increase.  An alternative explanation is that Mg and
Ca are ion-paired, complexed, or chelated to a much greater extent than Na.
Sulfate, present in inorganic or organic forms (ABS, LAS), and soluble
orthophosphates and organic polyphosphates are known to form complexes with
both Ca and Mg.  Since the composition of the exchange phase is regulated by
the concentration of free ions, an SAR calculated using total concentrations
would be expected to be low.  Therefore, the calculated ESR underestimates
the exchange phase composition when the irrigation water contains complex!ng
species.  Though ESR values are high throughout the treatment site, no
apparent infiltration rate decreases have been observed.

     Complications are apparent when discussing the fate of applied calcium.
As mentioned previously, analytical inaccuracies arise when measuring
exchangeable Ca in a calcareous soil.  For this reason, the U.S. Salinity
Laboratory [12] recommends that exchangeable Ca not be reported in calcareous
soils.  With this limitation in mind, the following discussion can only be
viewed as preliminary.

     Control and treatment site values for Ca are significantly different.
Unlike the previous exchangeable cations, calcium decreased after wastewater
addition, though the decrease was significant through the surface 30 cm (1
ft) only.  A decrease of 4.5 meq/100 g Ca was observed at the surface.

     The relationship of Ca to Mg is puzzling.  The applied wastewater had a
molar Mg/Ca ratio of approximately 2.  Given that most soil colloids have
nearly equal exchange affinity for Ca and Mg, it would be expected that the
ionic ratio of exchangeable Mg/Ca would be approximately the same as that in
the wastewater.  As is observed in Table 25, the addition of wastewater
increased the Mg/Ca ratio in the surface 100 cm (39 in.) to values greater

                                     80

-------
than 1, but reduced the ratio at 300 cm (10 ft).   This suggests that Mg was
selectively replacing Ca in the surface 100 cm (39 in.)  of the soil.  The
newly mobilized Ca traveled downward and then appeared to exchange with Mg at
300 crn (10 ft) resulting in a lower Mg/Ca ratio at 300 cm.

                     TABLE 25.   EXCHANGEABLE Mg/Ca RATIO
                                    Control  Treatment
                           Depth, cm   sites    sites
0-16
25-35
95-105
295-3905
0.3
0.5
0.8
1.7
5.4
115.7
1.3
0.8
                           cm x 0.3937 = in.

     Several hypotheses can be advanced to explain this ion-selective
behavior.  A partial  list of hypotheses is presented below.   A combination of
several is not ruled out.

     1.   Analytical  discrepancies

          a.   When calcium carbonate is present in a sample, reported CEC
               values may be too low, and exchangeable Ca too high.

          b.   In treatment site samples, reported values for exchangeable Ca
               may be too low because orthophosphate in the  acetate extract
               was not compensated for in flame atomic absorption
               spectrophotometry. Releasing agents such as Lanthanum or
               Strontium were not added.

     2.  Chemical interactions

          a.   Loss of Ca in the effluent by precipitation of a calcium
               phosphate species in the upper regions of the profile.

          b.   Selective adsorption of Mg in the upper regions of the soil
               profile by ligand combination.  The amino-carboxylate of ami no
               acids shows selectivity for Mg.  A significant amount of this
               ligand would be present only if there is a sufficient amount
               of decomposing protein with exposed peptide linkages.

          c.   Differential chelation, complexing, or ion-pairing of Ca and
               Mg in the solution phase.  This requires a ligand(s) of high
               concentration that selectively chelates to or is selectively
               coordinated by Ca.  Mg would then remain as a free ion  able
               to compete with Ma and K for a position on the exchange phase.
               Organic phosphates might be one such ligand,  though it is
               questionable whether they exist in concentrations that are
               sufficient to make this a complete explanation.

                                     81

-------
     3.  Historical

          a.   Effluent composition has fluctuated over time and effluent
               samples are not representative.

Heavy Metals

     Several sorption mechanisms have been proposed to describe soil  uptake
of heavy metals from a percolating solution.  Specific adsorption and
interfacial precipitation are thought to be the controlling mechanisms at
lower or trace amounts in the soil-water system.

     Bulk or noninterfacial precipitation of hydroxides, hydroxy-carbonates,
sulfides, or phosphates can be a controlling mechanism at any level  of a
particular metal, depending on the activity or concentration of the  nonmetal
precipitant.  At very high metal levels, cation exchange may be a controlling
mechanism [35, 47, 53-57].  In some cases several  processes may be operating
simultaneously.

     The extent of heavy metal sorption can be modified by several factors
including surface area of the sorbent, charge per unit area of sorbate, pH,
temperature, concentration of metals, and the presence of ion-pair complex
and chelate formers [47, 49, 57].  Iron and manganese are metals that also
undergo oxidation-reduction reactions and are greatly affected by biological
activity [58].

     The potential hazards of the heavy metals to man make them a critically
important variable to monitor in any land treatment system.  Where domestic
wastewater is applied [59] in rapid infiltration systems, accumulation in the
soil and leaching to groundwater should be assessed.

     The method of heavy metal analysis was a DTPA-extraction.  This
procedure is thought to vary in extraction efficiency, ranging from  only  a
few percent for iron and manganese up to 50 to 60% of the labile Cd.
Therefore, unlike "total-metal" analyses, DTPA-extractable metals cannot  be
used for mass balance calculations, unless the efficiency of extraction for
each metal is known.  The DTPA method is more sensitive to changes in metal
status in soil, and was therefore used for the following qualitative
interpretations.

     Seven of the nine monitored metals showed significant increases after
wastewater treatment, with p values ranging from 0.047 to 0.000.  Only lead
failed to show any significant differences between treatments.  Chromium  was
not included in any ANOVA because its concentration was below the detection
limit in all cases.  Depth-treatment interactions were not observed  for
copper, nickel, and lead.

     After wastewater application, DTPA-Fe increased by nearly a factor of 9
at the surface, and was also significantly greater at other sampling depths
throughout the profile (Figure 36).  There 1s a question of whether  this
increase is a result of accumulation of iron by wastewater addition  or the
result of alternating periods of wetness and dryness, resulting 1n both

                                     82

-------
reducing and oxidizing conditions.  Reduction of ferric iron to ferrous,
followed by oxidation of ferrous to ferric and subsequent precipitation'as a
hydrous oxide could lead to a less crystalline oxide, more soluble and more
extractable by DTPA.  There is no doubt, however, that iron was moving
through the soil profile as shown by a relatively high iron concentration in
the shallow and intermediate groundwater.  There was no indication from the
data whether iron was moving in the ferric or ferrous oxidation states or as
complexed, chelated, or stable soil forms.

     In contrast to iron, manganese was leached by the wastewater from the
surface 30 cm (1 ft) (Figure 37).  Surface manganese concentrations decreased
from 5.3 to 2.5 ppm.  Though the average increase is not significant  there
seems to be some redeposition at depth, expecially in treatment Sites 2 and
3.  In addition, there is evidence to suggest that manganese entered the
shallow and intermediate depth wells (see Section 9).  Preliminary soil
analyses (using "total-Mn") indicated that not only was there a diminution in
extractable manganese, but there was also significant loss of "total-Mn" in
the 0 to 16 and 25 to 35 cm (0 to 6 and 10 to 14 in.) samples at the
treatment sites.
     so
    100 ?
    1 90
 in  200
    290
    300
            DTPA-IRON, ppm

          90   100  190  200  290
         —I	1	1	1	1—i	1	1	1—
       DTPA-MANGANESE, ppm
                      LEGEND

                 D CONTROL SITES

                 O TREATMENT SITES
 too
                                             190 •
                                          u  200 •
                                             290 •
                                             300 •
       1.0  2.0  3.0  4.0   3.0
Figure 36.  Vertical distribution
of DTPA-extractable iron.
Figure 37.  Vertical distribution
of DTPA-extractable manganese.
                                    83

-------
     One explanation of this behavior is that the influx of carbon  (248  mg/L
as TOO and its subsequent oxidative metabolism by microorganisms could  have
created local anaerobic regions.  Within these regions, there is a  demand  for
alternative electron acceptors.  Nitrate ion, if available, represents a
suitable alternative to oxygen.  Tetravalent manganese in soil  oxides can
also serve as an electron acceptor for oxidative microbiological metabolism
of organic matter.  The result is reduction of manganese to the more soluble
divalent form, which can be lost from the surface by leaching.   Redeposition
would occur when and if a more oxidizing environment is encountered.  Because
many heavy metals are sorbed by manganese oxides, loss of these oxides from
the profile may have serious consequences.  Increased manganese
solubilization could lead to the mobilization of formerly sorbed metals  and
could diminish the capacity of the soil  to sorb metals entering the
wastewater.  Lehman and Wilson [60] and Ng and Bloomfield [61]  reported
similar behavior and attributed heavy metal mobilization to reducing
conditions caused by fermenting organic matter.

     Nickel and cobalt have similar geochemical behavior.  Both tend  to
associate with the hydrous oxides of manganese and iron.  Manganese oxides
are thought to be the major control, regulating cobalt in some  soils [57].
It is interesting to note that both cobalt and nickel did not increase in  the
surface 30 cm (1 ft) of the treatment site where manganese was  lost, but did
increase at 100 and 300 cm where Mn accumulated (the relationship  is not
statistically significant).  Average DTPA-extractable cobalt was six times
higher in treatment plots at 300 cm (10 ft) than in control plots  at the same
depth, 0.01 ppm versus 0.06 ppm (Figure 38).  Similarly, average nickel
values doubled from 0.17 ppm to 0.40 ppm at the 100 cm (39 in.) depth  (Figure
39). Shallow groundwater concentrations of both nickel and cobalt were
statistically greater than background levels measured in the offsite control
wells, and suggests that both metals were passing through the soil  profile.

     DTPA-extractable cadmium and zinc values are summarized in Figures  40
and 41, respectively.  For cadmium, wastewater application caused  a
significant increase only at the 30 cm (1 ft) depth where its average
concentration increased from 0.015 ppm in controls to 0.053 ppm in  treatment
sites.  The apparent increase at the surface is not significant.

     DTPA-extractable zinc significantly increased in wastewater treatment
profiles even at 300 cm (10 ft), where the average control value was 0.06
ppm, compared to an average treatment site value of 0.24 ppm.  This suggests
that some zinc passed down to and perhaps through the 300 cm (10 ft)  depth.
Groundwater zinc concentrations (Section 9, Table 26) were also high,  further
suggesting that zinc was moving downward.  The relatively higher mobility  of
zinc contrasts with the behavior of cadmium, even though many aspects  of the
chemistry of zinc and cadmium are similar [62].  This apparently higher
mobility of zinc may be related to its higher input concentration  in  the
wastewater.

     DTPA-extractable copper behaved in a manner similar to cadmium, showing
a most pronounced accumulation at the surface, where average values increased
                                     84

-------
             DTPA-COBALT, Ppm



            0.0*3  0.06 0.09 0.12


             ' '        '
     1 00
     1 50 -
  iu  200 •
     250
     300
             LE6END


          O  CONTROL SITES


          O  TREATMENT SITES
   Figure  38.   Vertical distribution

   of DTPA-extractable cobalt.
                DTPA-NICKEL, ppm



               0.5  1.0   1.5   2.0
                                                     90
                                                    100
                                                    1 50
                                                 uj  200
                                                    250
                                                    300
      n™ 39'   Vertical distribution
   of DTPA-extractable nickel.
           DTPA•CADMIUM, ppm



          0.05 0.10 0.19  0.20
   100
   19.0
ui  200
   290
   300
            LEGEND


        O  CONTROL SITES


        O  TREATMENT SITES
                                               90
                                              toot
    150
 *
3:
»-


u   200
O





    290





    300
            DTPA-ZINC, ppm


          2.0  4.0  6.0   t.O 10.0
 Figure 40.  Vertical distribution
 of DTPA-extractable cadmium.
     Fl9nTn«41'  Vertical  distribution
     of DTPA-extractable zinc.
                                        85

-------
 significantly from 3 ppm in controls to 7 ppm in treatment sites (Figure 42).
 Although treatment site values were larger than controls below 30 cm (1 ft),
 the increase is not significant.  Copper concentrations in the shallow and
 intermediate depth groundwater samples were nearly equal to input values.
 This may indicate that copper was moving unimpeded through the profile,
 possibly as a metal  chelate or an ion pair.  It may also indicate that
 effluent copper concentrations were not greatly different from levels in the
 soil-water controls.

      The behavior of lead is different than any of the other metals discussed
 previously.  Wastewater application did not cause any significant differences
 (Figure 43) in concentration.  This may reflect the small imput of lead or it
 might indicate that lead is passing through the profile to the groundwater
 below.
    50
   15*
   288
   258
           DTPA-COPPER ,  ppm
        1.02.83.84.05.88.07.0
        -I	1	1=—I	1	1	1	
          OTPA-LEAD, pp>
                                               90
                                              tao
                                              150
                                           ui  200
                                              290
                                              300
                                LEGEND

                           O CONTROL SITES

                           O TREATMENT SITES
Figure 42.  Vertical distribution
of DTPA-extractable copper.
Figure 43.  Vertical distribution
of DTPA-extractable lead.
                                     86

-------
 Agricultural Potential of Soils Treated with Heavy Metals

     Monitoring the potential for accumulation of heavy  metals within the
 food chain  involves an evaluation of the ionic activities  at  the  plant root
 surface, the amount of labile metals relative to both total composition and
 solution activities, and relative intensity effects in which  the  availability
 of one  ion  is affected by that of other ions.  Plant uptake of an ion from
 soils is affected by inherent differences among species  and varieties within
 species, ion interactions, and soil-plant interactions [63].

     Copper, cadmium, and zinc have been the metals of primary concern in
 most wastewater disposal  systems [64].   However, copper  and zinc  do  not
 usually approach toxic levels in the ultimate food chain as a result of
 wastewater  disposal  since plant toxicities occur before  these metals reach
 levels  that would be harmful  to animals and humans.  Cadmium  poses the
 greatest concern to the ultimate food chain since it is  readily taken up by
 plants  and  accumulates in the vital  organs of animals ana  humans  eating these
 plants  [64].  One way to offset this potential  problem is  to  have in solution
 metals whose chemistries are similar to that of cadmium  and can successfully
 compete for plant uptake.                                      a^i.«aiui iy

     Research has shown that at low cadmium levels, increasing the relative
  i±nh! ni™l
-------
            TABLE  26.   COMPARISON OF SOIL MICROELEMENT CONTENT AFTER
                  LONG-TERM SLUDGE AND WASTEWATER APPLICATION
                                       Range of DTPA - extractable metals, ppm
Source
Chaney [65 ]

Hollister
(Surface 35 cm)
Condition
Sludge treated
Control
Wastewater treated
Control
Zn
1.3-95
2.7-7
1.1-11
0.12-1.6
Cd
0.10-27
0.08-0.16
0.04-0.24
0.01-0.12
Cu
1.4-100
1-4
1.4-8.2
0.35-4.0
Ni
0.2-88
0.2-0.6
0.33-2.4
0.24-0.62
             TABLE 27.  COMPARISON OF SOIL DTPA-EXTRACTABLE HEAVY
            METAL WITH HEAVY METAL CONTENTS OF SELECTED CROPS [65]

Location

F-0416
F-0418
F-0420
F-0422
F-0424

Sc
F

6.
6.
6.
6.
6.


n i —
iH

9
6
4
2
1

Soil



DTPA-extractable, air dry soil
Zn

8.0
6.8
61.8
36.6
25.8
Cd

1.36
1.06
8.4
5.8
3.8
Cu

4.6
5.1
25.3
15.6
10.1
N1

0.8
0.9
4.1
3.0
2.0
Zn

30.3
32.8
49.8
46.0
45.6

Crop


Grains, dry crop
Cd

0.22
0.10
2.13
1.32
1.68
Pd
Oat
0.71
0.61
0.56
0.56
0.50
Cu

4.0
4.2
4.7
4.3
4.8
N1

2.1
1.5
7.5
5.6
5.8
Red Clover
F-1681
F-1682
F-1683
F-1684

F-0749
F-0750
F-0751
F-0752
F-0753
F-0754

F-0758
F-0759
F-0760
6.
6.
6.
5.

6.
5.
5.
6.
6.
6.

5.
5.
5.
6
6
1
9

4
5
9
2
3
4

2
0
0
62.8
30.1
24.6
24.6

8.8
13.6
9.6
5.9
3.7
3.9

7.1
6.4
10.2
8.9
4.5
3.4
3.0

0.22
0.33
0.23
0.17
0.11
0.11

0.13
0.17
0.22
29.0
10.8
8.6
7.4

5.3
8.1
5.2
4.2
2.3
3.0

3.8
4.1
7.1
4.2
2.3
2.2
2.2

0.8
1.2
0.6
0.5
0.4
0.5

1.2
0.9
O.B
43.4
57.6
45.5
50.6

48
67
56
51
45
44

19
18
16
0.88
0.92
0.60
0.66

0.30
0.19
0.23
0.23
0.18
0.37

0.23
0.56
0.43
2.3
2.4
1.6
2.6
Soybean
0.50
0.84
0.74
0.60
0.89
0.57
Corn
0.87
0.78
0.77
7.7
10.8
7.9
7.2

12.0
14.3
12.8
13.9
11.8
12.7

1.8
2.0
1.7
2.2
1.6
1.8
2.3

1.8
2.4
2.8
2.0
1.5
1.4

0.7
0.4
0.4
     Heavy metal uptake by plants appears to  be  a  function  of  available
metal, pH, and plant species.   Uptake tends  to  increase  as available metal
increases and decreases with a rise  in pH.  Metal  enrichment will  vary from
crop to crop.  Though conclusions are confounded by  these interacting
factors, Chaney et a].. [65] found from these  and other  data that the uptake
of zinc) cadmium, and nickel was accelerated  by  sludge  incorporation
especially at low soil pH, and that  copper  and lead  uptake  generally was
unaffected by sludge addition.  Most importantly,  no phytotoxicity was
observed in crops ordinarily grown on farms where  sludge  had been used.

-------
     In light of these findings, it seems reasonable to conclude that the
levels of DTPA-extractable metals will  not adversely affect the future
agricultural potential of the Hoi lister rapid infiltration site.  Surface
soil pH values of 6.3 to 7.6 will favor reduced metal  uptake and soil  DTPA-
extractable metal concentrations are low relative to soils having higher
metal loading rates without observed plant toxicity.  Any crops grown on the
site should be monitored for heavy metal  uptake, however.
                                    89

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                                  SECTION 9

                      GROUNDWATER QUALITY INVESTIGATION
INTRODUCTION
     The hydrogeologic investigation determined that the shallow groundwater
table beneath the rapid infiltration basins was hydraulically responsive  to
percolating wastewater.  The shallow groundwater was sampled when underground
detention time was at a minimum by taking samples at the maximum water level
(See Figure 13).  The results from Wells 3A and bA, in the shallow groundwater
aquifer, represent renovated water quality after 7 m (22 ft) of percolation
through the unsaturated soil matrix.

     The onsite intermediate wells (IB and 3B) did not show a definite water
table response to effluent application (Figure 14), and groundwater quality
did not convincingly indicate movement of effluent to this level.  The onsite
deep wells (1C and 2C), similarly, did not indicate any influence of applied
wastewater on deep groundwater as observed from water table fluctuations  and
groundwater quality data.  All results were compared to offsite control
wells, upgradient and downgradient of groundwater movement in the regional
groundwater aquifer.

     The results of the groundwater sampling program are shown in Table 28.
The values represent the arithmetic average of four grab samples taken, over
the study period, according to the procedures stated in Section 6.   The
range, standard deviation and coefficient of variation for the individual
samples are listed in Appendix C.  Chemical and bacterial analyses were
performed according to Standard Methods [9].  The results of effluent
sampling accompany the groundwater quality results for comparison.

STATISTICAL ANALYSIS OF GROUNDWATER DATA

     For purposes of analyses, well samples were grouped into four categories-
shallow (A), intermediate (B), deep (C), and controls.  Well 4C was
originally established as an upgradient control well.  The validity of this
assumption was tested for every parameter.  Unless there was a significant
difference between Well 4C and the downgradient control wells, (at thea =
0.10 level), it was considered to be a control well for that particular
chemical parameter.  Data collected over time were viewed as replicate
samples.

     Once properly grouped, a one-way analysis of variance (ANOVA)  was
performed using the SPSS statistical package [67].  Data reported as "less


                                     90

-------
TABLE 28.  AVERAGE EFFLUENT AND GROUNDWATER QUALITY RESULTS
                    mg/L Except as Noted
Groundwater'
Effluent
Constituent Range Av
COO 546-1.029
BOD.' 134-414
TOC 240-264
CCE
Total N 29.7-58.5
NH4- - H 19.7-44.0
M org. 6.7-21.8
NO, - N 0.16-0.6
Total P 10.0-21.5
P04 - P 9.0-13.2
Total conforms, 4.6 x 10'- -,
count/100 BL 92 x 10' " •
Fecal Conforms, 3.5 x 10'- .,
count/100 ml 24 x 10' "'
pH, units 7.0-8.1
TDS 1,016-1,593
Conductivity, J>H0.Zi480
Alkalinity 416-465
B 0.95-1.8
F 0.3-1.1
55 206-328
N« 196-391
Ca 31-71
Kg 27-76
K 10.9-16.0
Cl 185-490
S04 161-250
Ag <0. 005-0. 012
As
Ba <0.1-0.24
Cd <0. 001-0. 008
Co <0. 006-0.008
Cr <0. 004-0. 036
Cu 0.019-0.070
Fe 0.14-0.82
Hg
Hn 0.055-0.094
N1 0.015-0.092
Pb 0.012-0.12
Se
In 0.010-0.090
SAR 4.67-8.08
a. Average of 4 grab samples.
b. Depth of xell screen • 7.5-10.6
c. Depth of Mil screen • 19.5-24.0
d. Dtpth of Mil screen 48 •.
e. Average of twelve 24 h composite
f. Average of three 24 h composite
g. Single grab sample.
Shillo»b
erege*
706
220
248f
"
40.2
25.3
14.5
0.43
12.4
10.5
6 x 10*
4 x 10*
7.3
1,208
1,790
446f
1.4
0.7
274n
262
54
64
12.9
284
213
<0.008
<0.01
<0.13
<0.004
<0.008
<0.014
0.034
0.39
<0.001
0.070
0.051
0.054
<0.001
0.048
5.71

*.
».

samples
temples.

3A
46
6
10'
2.2
3.8
<0.4
2.2
1.2
8.0
6.8
1.1 x 10'
156,000
7.5
1,282
1,710
538
1.2
1.1
15
249
100
71
13.0
281
164
<0.006
<0.01
<0.11
0.007
<0.008
 1.281 • ft






















                            91

-------
than x" were input as x.  The option used was "ONEWAY," which tested the
hypothesis:

                              Ho: Ul=y2=u3=p4=y5

where y-|_4 are the means of the four groundwater groups, and yg is the
effluent mean.

     If the hypothesis could not be rejected at the a = 0.10 level, no
further statistical analyses were performed.  If rejection was warranted,
pairwise contrasts (t-test) were performed to isolate the sample(s) whose
mean was different.  The t-statistic was computed using both pooled and
separate estimates of variance.  Only if Cochran's "C" statistic proved
significant (ct= 0.10), indicating nonhomogeneous variance, was the latter t-
statistic used.  Sample output from SPSS ONEWAY is shown in Table 29.

COD, BOD, AND TOC

     COD, BOD, and TOC are indicators of the strength of organic compounds
present in wastewater and groundwater.  The chemical  oxygen demand (COD)  is a
measure of the oxygen equivalent of that portion of organic matter that is
susceptible to oxidation by a strong chemical oxidant.  The biochemical
oxygen demand (BOD) test measures the oxygen uptake in the microbial
oxidation of organic matter.  TOC is a measure of total organic carbon.
Bouwer and Chaney [68] stated that for land treatment, TOC may be a more
appropriate parameter than BOD and COD, since the latter two were developed
primarily for oxygen requirements in aquatic environments.  TOC can also be
used as a gross indicator of persistant organic compounds that remain when
BOD and COD are completely removed [69].  In addition, TOC can be used as  a
measure of the energy (carbon) available for denitrification.  Actual
observed carbon requirements for effective denitrification vary in the
reported literature [70, 71].

     In a rapid infiltration system, the reduction of BOD, COD, and TOC  is
accomplished largely by the adsorption and subsequent biodegradation of
organic compounds.  Lance et al. demonstrated that anaerobic biological
decomposition can be as effective as aerobic reactions for soil columns under
conditions of continuous flooding [72] or long periods of flooding (9 days)
followed by a shorter recovery period of 5 days [71].

     The COD of the Hoi lister primary effluent ranged from 546 to 1,029 mg/L
with an average concentration of 706 mg/L.  The average COD concentrations in
shallow Wells 3A and 5A were 46 and 50 mg/L, respectively.  These values
correspond to a 93% COD reduction after vertical percolation through 7  m (22
ft) of soil.

     Relative to the other deep wells, COD levels in Well 2C were higher by a
factor of 5 to 8 throughout the study period.  This resulted from well
contamination.  An organic mud which was used to maintain the borehole during
well construction was observed in each groundwater sample from Well 2C.  When
Well 2C is excluded, significant differences between intermediate, deep,  and
offsite control wells are not observed.  This supports the contention that

                                      92

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                        TABLE  29.   SAMPLE  OUTPUT  FROM  SPSS  ONEWAY
VARIABLE:  TOTAL NITROGEN
                              ANALYSIS OF VARIANCE
SOURCE D.F. SUM Of SQUARES MEAN SQUARES
BETWEEN GROUPS 4 11661.1698 2915.2922
WITHIN GROUPS 46 1123.6402 24.4270
TOTAL 50 12784.8086
GROUP
GRPOl
GRP02
GRP03
GRP04
GRP05
TOTAL


STANDARD
COUNT MEAN DEVIATION
10 3.3500 2.3538
9 7.2889 4.2040
8 2.3000 2.3821
12 4.5250 4.3650
12 39.8666 7.8803
51 12.7490 15.9905
FIXED EFFECTS MODEL 4.9424
RANDOM EFFECTS MODEL 16.0494
STANDARD
ERROR
0.7443
1.4013
0.8422
1.2601
2.2748
2.2391
0.6921
7.1775
MINIMUM
0.
1.
0.
0.
29.
0.


2000
3000
4000
2000
7000
2000


F RATIO
119.347
MAXIMUM
8.
14.
7.
12.
58.
58.


7000
5000
9000
2000
3000
3000


95
1.
4.
0.
1.
34.
B.
11.
-7.
PCT
6662
0574
3085
7516
8697
2516
3559
1786
F PROB.
0.0000
CONF
TO
TO
TO
TO
TO
TO
TO
TO
INT FOR
5
10
4
7
44
17
14
32
MEAN
.0338
.5204
.2915
.2984
.8735
.2464
.1421
.6766
CONTRAST COEFFICIENTS MATRIX
CONTRAST
CONTRAST
CONTRAST
CONTRAST
CONTRAST
CONTRAST
CONTRAST
CONTRAST
CONTRAST
CONTRAST 10
CONTRAST 1
CONTRAST 2
CONTRAST 3
CONTRAST 4
CONTRAST 5
CONTRAST 6
CONTRAST 7
CONTRAST 8
CONTRAST 9
CONTRAST 10
GRPOl

  1.0
  1.0
  1.0
  1.0
  0.0
  0.0
  0.0
  0.0
  0.0
  0.0
GRP02
 -1.0
  0.0
  0.0
  0.0
  1.0
  1.0
  1.0
  0.0
  0.0
  0.0
                        GRP03
                                   GRP05
0.0
-1.0
0.0
0.0
-1.0
0.0
0.0
1.0
1.0
0.0
GRP04
0.0
0.0
-1.0
0.0
0.0
-1.0
0.0
-1.0
0.0
1.0
0.0
0.0
0.0
-1.0
0.0
0.0
-1.0
0.0
-1.0
-1.0
            VALUE  S.
         -3.9389
           1.0500
         -1.1750
         -36.5166
           4.9889
           2.7639
         -32.5777
         -2.2250
         -37.5666
         -35.3416
                                     POOLED VARIANCE ESTIMATE
                                 ERROR    T VALUE   D.F.      T
              2.2709
              2.3444
              2.1162
              2.1162
              2.4016
                1794
                1794
                2559
                2559
                    2.0177
 -1.735
  0.446
 -0.555
-17.256
  2.077
  1.268
-14.948
 -0.986
-16.653
-17.516
46.0
46.0
46.0
46.0
46.0
46.0
46.0
46.0
46.0
46.0
PROB.

0.090
0.656
0.581
0.000
0.043
0.211
0.000
0.329
0.000
0.000
                                                            • SEPARATE  VARIANCE ESTIMATE
                                                     S. ERROR     T VALUE   D.F.    T PROB.
1.5868
1.1240
1.4635
  3935
  6349
  8845
  6718
  5156
  4257
                                                                        2.6005
 -2.482
  0.934
 -0.803
-15.256
  3.051
  1.467
-12.193
 -1.468
-15.487
-13.590
12.3
15.1
17.4
13.3
12.9
17.7
17.5
17.5
13.8
17.2
0.029
0.365
0.433
0.000
0.009
0.160
0.000
0.159
0.000
0.000
                                                   93

-------
COD concentrations in both the intermediate and deep aquifers are not the
result of wastewater application.

     The BOD of the effluent ranged from 134 to 414 mg/L and averaged 220
mg/L.  The average BOD concentrations in Wells 3A and 5A were 6 and 13 mg/L,
respectively.  A 96% reduction in BOD was achieved.  BOD levels in all
aquifers were not statistically different.  Shallow aquifer concentrations
were indistinguishable from background concentrations.  Anomolous BOD
behavior was not observed for Well 2C.

     The TOC was measured only during the last sampling period.  The range of
240 to 264 mg/L and average of 248 mg/L was for three composite effluent
samples.  The TX concentrations of Wells 3A and 5A were 10 and 11 mg/L,
respectively, for one grab sample in March 1977.  A 96% reduction of TX in
the shallow groundwater is indicated.  All wells have statistically equal TOC
concentrations suggesting that even "refractory" organic compounds have been
removed to background levels.  More data on the movement of refractory
organic compounds are needed, however, to base any conclusions.

     The COD, BOD, and TOC results indicate that effective aerobic
decomposition of organic matter was occurring.  The wastewater application
cycle allowed for the reduction of organic constituents to background levels.
Hoi lister removal efficiencies compare favorably with those reported for the
Fort Devens, Massachusetts, rapid infiltration site having 31 years of
continuous operation.  At Fort Devens, primary effluent COD and BOD
concentrations of 192 and 112 mg/L were reduced to 27 and 5.9 mg/L,
respectively [73].  Wastewater percolates approximately 5.4 m (18 ft) through
soils with physical  and hydraulic characteristics similar to those at
Hoi lister.

RESIDUAL ORGANICS (CARBON CHLOROFORM EXTRACT)

     Organic compounds present in groundwater were adsorbed onto activated
carbon and extracted with chloroform [74].  This procedure was developed for
high quality water and the effluent was not analyzed.  The large sample
volume (55 litres),  time consuming adsorption procedure, and costly solvent
extraction limited the number of samples tested.  Carbon chloroform extract
(CCE) values are expressed as mg/L, based on the total weight adsorbed onto
the carbon divided by the volume of water passed through the carbon sample.
While no determination of removal  efficiency was possible, it can be inferred
from the COD, BOD, and TX results that wastewater application has no
influence on the intermediate or deep groundwater.  The CCE levels of onsite
and offsite intermediate and deep wells were comparable; however, inadequate
sample replicates prevented statistical  relationships between CCE and other
organic pollution indicators.

NITROGEN

     Typical, medium  strength domestic wastewater may contain 40 mg/L of
nitrogen of which 25 mg/L is in the form of NH.-N and 15 mg/L is 1n the
organic form [75].  A review of several  rapid infiltration projects indicates
that the concentration and form of nitrogen being applied to the soil is the

                                     94

-------
 same  after  primary or secondary treatment [69, 76-78].   Ammonium and organic
 nitrogen  applied to soils at rapid infiltration sites are almost completely
 converted to the nitrate form  [69, 76-78].  The availability of oxygen is
 critical  to the conversion process.  Short and frequent application cycles
 from  0.5  to 3 days flooding followed by 5 to 14 days before reapplication '
 maximize  nitrification [69, 77, 78].                                     '

      In addition to nitrification, denitrification is an important conversion
 process.  Organic carbon is needed to supply energy to denitrifying bacteria
 The level of organic carbon in the supplied wastewater is critical  to the
 removal of  the highly mobile nitrate ion.  Lance and Whisler observed that
 nitrate from a percolating wastewater was removed by denitrification [79]
 The denitrifying bacteria are facultative anaerobes which use nitrate-
 nitrogen  as an electron acceptor when oxygen concentrations become very low.

      In the denitrification reaction, where glucose is used as a caroon
 source, 3.2 grams are required for each gram of nitrogen denitrified (C-N «

 ~1'^1  h?3CSnJn?«l!!!1?ler obsSrved that stabilized municipal wastewater (C:N
 * 1.2.3)  did not contain enough unstabilized organic matter to denitrify
 wastewater  applied to soil columns [71].  These laboratory results were
 verified  at the Flushing Meadows rapid infiltration project [69]   On the
 other hand, it is very probable that a high BOD wastewater does denitrify
 rapidly when applied to soil.  Law et aK reported 83 to 90% removal of total
 nitrogen  from overland flow treatment of high BOD cannery wastes [80]   Lance
 also  demonstrated that with the addition of 150 mg/L glucose (C-N * 5*n
 soil  columns intermittently flooded with secondary sewage water'realized'a
 90% nitrogen reduction [71].   Nitrogen removal decreased to 60% when the
 carbon concentration was 80 ppm (C:N * 2.7:1) [71].  At Hoi lister the C N
 ratio was approximately 6 to 1, a condition favoring denitrification.

      In addition to the C:N ratio in wastewater, the distribution of carbon
 in the soil  profi  e influences the location where denitrification might take
 place.  At the Hollister rapid infiltration site, soil  organic matte? was
 highest near the surface and declined progressively with depth (See Table
 19).  Gilmore et_al_.  showed that a flooded surface soil  containing 0 9% total
 carbon denitrified added nitrate readily without organic amendments, but thl
 subsoil containing 0.48% total  organic carbon failed to denitrify unless an
 available organic  substrate was supplied [81].  Therefore, the zone of most
 active denitrification is likely to be near the soil  surface in spite of its
 proximity to the atmosphere.   This has been demonstrated in field experiments
 by Rolston et a .  who observed maximum rates of production of NO and N
within the WTO  cm (4 in.)  [82].  Lance et al. [83] a sS observed thai
 denltrf cation takes place near the soil  luTfTce.   By  monUorin  reSSx
 potentials versus  depth in sewage flooded soil columns   It was Sown SL+
 denitrification zone  was  established in the top 20  S (8 ?n ) or less [83]

     At Hollister,  a  93% reduction in total  nitrogen was achieved as thP
wastewater passed  from the surface to the shallow water table   NTtrate-N
levels in the shallow water table are approximately 1  ppm and therefore Led
no immediate contamination problem to permanent groundwater supplies
                                    95

-------
     Denitrification is believed to be the primary removal  mechanism.
Favorable conditions for conversion exist at the infiltration site,  including
availability of an energy source, temporary anaerobism due  to soil  flooding,
adequate detention time due to moderate infiltration rate,  and a near  neutral
pH.  The infiltration rate at Hoi lister was in the range reported by Lance
and Whisler for good nitrogen removal  [79].

     Concentrations of all nitrogen species in the shallow  water table were
statistically indistinguishable (t-test, a = 0.10) from the corresponding
nitrogen levels in the offsite control wells.

     Higher concentrations of nitrate-N and total-N were observed in the
intermediate wells and were comparable to offsite control wells.  This
suggests that the nitrogen in the intermediate aquifer was  derived from a
source other than the surface applied wastewater.

PHOSPHORUS

     Concentrations of phosphorus in municipal wastewater§3can range from 1
to 40 mg/L, occurring mostly as inorganic phosphorus (PO.  ) [84, 85].  The
effluent concentration will be a function of influent concentration and the
degree of removal during treatment.  Primary and secondary effluents may have
similar concentrations.  For example, the secondary effluent applied at
Flushing Meadows ranged from 10 to 15 mg/L P04-P  [69]while the inorganic
phosphorus concentration for Hoi lister's primary effluent averaged 10.5 mg/L
P04-P.

     Phosphorus removal in rapid infiltration systems has been studied in the
Flushing Meadows project [23, 69, 76].  The  phosphorus concentration in the
wastewater averaged 15 mg/L in 1969, but decreased to about 10 mg/L for the
period 1970 to 1972.  Phosphorus removal increased with  an increase in travel
distance and residence time in the soil profile.  A travel  distance of 9 m
(30  ft) removed about 70% of the phosphorus  in 1969, but removal efficiency
was  reduced to about 30% in 1970 when a substantial increase  in flowrate
occurred.  With a flow distance of 100 m (330 ft), phosphorus removal
increased to about 90% and was greater with  an even longer travel distance.
After 5 years of operation and phosphorus  additions of nearly 48.0QO kg/ha
(43,000 lb/acre) the removal efficiency was  rather stable.

     Pratt [23] points out that  rapid infiltration systems require  sandy
soils that can sustain high water  intake rates and high  transmissivity in the
subsurface environment.  Therefore, no  layers with high  sorptive capacity for
phosphorus are likely to be encountered.   What little capacity  there  1s may
soon be saturated, and the retention will  then depend on mineralization  and
precipitation reactions.  One logical precipitant  is  the calcium supply  1n
the  wastewater.

     The coarse gravelly  soil at Flushing  Meadows  [69,  76]  is calcareous and
contains little or  no  iron and aluminum oxides.  Therefore,  1t  was  concluded
that P removal resulted  from  the precipitation of  calcium  phosphate species,
ammonium magnesium  phosphates, and other  insoluble compounds  [69].
                                      96

-------
      At  the Calumet, Michigan, rapid  infiltration  system, phosphorus removal
 continues to be from 89 to 97% efficient, though the system has  operated for
 over 88 years.  The influent concentrations of a raw municipal wastewater
 were reduced from 3.5 mg/L total -P  to 0.4 to 0.1 mg/L after 3.1  to 9 2 m (10
 to 30 ft) of vertical percolation,  respectively.   The soil  is a  poorly sorted
 gravel having an annual hydraulic loading of 34 m/yr (112 ft/yr)  [86],

      At Fort Devens, Massachusetts, [73] where a primary effluent has been
 a?PlleJ ^n^101 1njiltration basins  for over 30 years, removal  efficiencies
 of 86 to 90% were observed in observation wells located 80 to 150 m (262 to
 492 ft) from the infiltration basins.  Only 18% of the input phosporus
 remained after 1.5 m (5 ft) of vertical  percolation [73].  The total

                                                h*draulic loadi"9 ^s 27.1
     At the Lake  George Village rapid infiltration site, total  phosphorus in
 an  observation well 4.9 m (16 ft) below the surface averaged 0.9 mq/L  as
 compared to an input level of 2.1 mg/L.  Unchlorinated secondary effluent is

 43Pl1?140°ft)fUr         SaPd bedS dt dn annUal hydraulic aPPHcation rate of
 nn cAL5,0!l1sJf!> 85% °Vh(:  influent Phosphorus is inorganic orthophosphate
 (10.5 mg/L).   After percolation  through 6.7 m (22 ft) of soil to the shallow
 groundwater,  23  to 35% of the  input P was removed.  This figure is in c?ose
 agreement with the 30% removal ratio calculated for the upper 300 cm of soil
 pK*OT 1 I 6»

     There is no statistical evidence of phosphorus contamination in the
 intermediate  and deep water-bearing strati.   Intermediate,  deep  and offsite
 contro  we is indicate that average P concentrations were statistical ly
 indistinguishable at the o- 0.1 level.  Only the shallow water table
 responded to  wastewater addition.

     The ratio of P04-P/total  P  in the wastewater is comparable to the same
 ratio in the  shall ow\ater  table.  This suggests that there was St Elective
 removal  of morgamc-P relative  to organic-P  in the soil  profile   Both forml
 were entering the shallow groundwater.                  t»uiiie.  eotn terms

 FECAL COLIFORM AND TOTAL COLIFORM BACTERIA


 exrlhrfL^^^
 water  and groundwater.  The presence of fecal coliforms  is indic

 «^frg^%t ss^s.'ff^1B
 associated with domestic  wastewater.             H»«*«uiai  neaitn risks
     The  relative health  risks from pathogens at rapid infiitrati™
be greater than other land treatment techniques bemuse ?he sol I  J
highly transmissive and thus has less chance to retain
Chaney [68] point out that fecal  col iformblcteri fare  ne
after water has traveled  5 to 7 cm (2 to 3 in.) througth! !
                                   97

-------
 the  coarse  soils  at  rapid  infiltration  systems, which are necessary to
 maintain  infiltration  and  transmissivity, may require on the order of 30 m
 (100 ft)  to completely remove  fecal coliform bacteria.  With adequate travel
 distance, effective  removal  of fecal  coliforms has been demonstrated [69,
 73].

      Bacterial  removal  efficiency within the soil profile is a function of
 wastewater  application rate, subsurface  travel distance, solids buildup on
 the  soil  surface, the  type  of  organism,  soil type, moisture-retention
 capacity, soil  organic content, pH, temperature, sunlight, rain, degree of
 wastewater  contamination,  and  antagonism from the resident microbial flora.
 Under certain  favorable conditions, applied organisms may actually multiply
 and  increase in numbers.   In general, land treatment using intermittent
 application and drying periods results  in die-off of enteric bacteria
 retained  in the soil [23].

      Bouwer et al. [69] found  deeper  penetration of fecal coliforms below
 rapid-infiltration basins  after the basins were flooded following an extended
 drying or resting period.   This was probably due to reduced entrapment of E.
 coli  on the surface  of the  soil.  The clogging layer of organic fines that
 had  accumulated on the soil  during flooding, forming an effective filter,
 partially decomposed after  drying.  A more open surface was created,
 resulting in a less  effective  filter when flooding was resumed.  In addition,
 the  bacterial  population in the soil  undoubtedly declined during drying
 because the nutrient supply was discontinued.  Consequently, there was less
 competition from  the native soil bacteria, and hence greater survival of the
 fecal  coliforms when flooding  was resumed.  As flooding continued, however,
 fine suspended solids  accumulated again on the soil  surface.  The native soil
 bacterial population also  increased.  This resulted in increased retention of
 E. coli and a  return to fecal  coliform levels of essentially zero in
 renovated water sampled from a depth of 9 m (29 ft).  Almost all fecal
 coliform  removal  took  place in the first 1 m (3 ft)  of soil.

      At the Fort  Devens, Massachusetts rapid infiltration system, analysis of
 groundwater samples  for fecal  coliform bacteria proved negative in
 observation wells located 60 to 100 m (197 to 328 ft) from the application
 area  [73].   Total coliform  densities  in the unchlorinated effluent ranged
 from  18 to  53  x 10^  per 100 ml.

      At Hoi lister, fecal coliform removal approached 99% consistently in the
 shallow groundwater  Wells 5A and 3A after a total percolation distance of 7 m
 (22  ft).  Although fecal coliform densities were substantially reduced from
 levels  in the  applied  effluent, they were significantly higher than levels in
 the  intermediate and deep wells.  In general, fecal  coliforms were absent in
 intermediate and deep wells.   The exception was intermediate depth Well  3B
which averaged  11  per  100 mL for two samples, with three negative samples.
This could  have resulted from  "short-circuiting" into the intermediate
aquifer from either  the shallow water table or direct surface contamination.
No fecal coliforms were detected in intermediate Well IB.  Total coliform
bacteria,  which includes fecal  coliforms plus coliforms orginating from other
sources, were also monitored.
                                      98

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     The relationship between fecal and total  coliform counts gave some
indication of bacteria originating from other than human sources.   Total
coliform bacteria removal ranged from 96% in Well  5A to 99+% in Well  3A after
7 m (22 ft) of percolation.  The uncharacteristically high total  coliform
densities in Well 2C are believed to be the result of residuals from  the
drilling operation which were not completely flushed from the well during
construction.  This material  was present in each sample withdrawn  from Well
2C over the sampling period,  but was not present in samples from other wells.
High fecal  coliform counts in Well 9C, an offsite  control well, were  noticed
in one sample out of two taken during the study period.

     The large heterogeneity  of variances in the coliform data made standard
statistical comparisons meaningless.

pH

     The pH of wastewater can be modified by the soil's buffering  capacity
and by the chemical and biological reactions which take place within  the soil
profile at a rapid infiltration site.  Wastewater  which contains organic
acids can show an increase in pH as it moves through the soil because the
acids are biodegraded and lost from the system [68].  On the other hand, for
biodegradable material, pH may decrease because soil microbial activity'
produces CC^ and organic acids [68].

     At the Flushing Meadows  project, the pH of sewage effluent  was  about 8
whereas that of the renovated water was approximately 7.  This pH drop was
attributed to the bacterial production of C02  and  organic acids.
Nitrification and removal of  carbonates would also reduce the pH [69].

     At the Lake George Village, New York, rapid infiltration site, secondary
effluent pH was 6.94 while that of the renovated water in a well 4.9  m (16
ft) directly beneath an infiltration basin was slightly lower at 6.37 [87]
Production of organic acids and carbon dioxide by  soil bacteria and
nitrification of ammonium were thought to be responsible for the slight oH
drop [88].                                                          3   p

     At Fort Devens, Massachusetts, the pH of soil  water extracted by suction
lysimeters at 1.5 m (5 ft) increased slightly in one lysimeter and stayed the
same in another [73].   Control site soil  pH ranged from 4.6 to 5.2 whereas
the treatment bed pH ranged from 5.7 to 6.2 [73].  Comparison of effluent and
groundwater pH directly beneath spreading basins at a depth of 24.4 m (80 1
ft) showed a drop from 7.0 to 6.8 standard units [73].
     At the Whittier Narrows and Rio Hondo test basins, surface applied
secondary effluent had pH values of 8.0 and 8.30, respectively   After
percolation through 1.8 m (6 ft) of soil  previously used for groundwater
recharge of stormwater runoff, the pH had dropped to 7.78 and 7 90
respectively.  The pH reduction was attributed to carbon-dioxide production
and/or nitrification in the soil matrix [89].                     K

     At Hoi lister, a significant difference between the average pH of the
primary effluent (7.3) and the pH of the  shallow groundwater (7.6) was
observed.  However, the pH of the shallow groundwater was indistinguishable

                                     99

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(t-test, a= 0.10) from that of the offsite control wells (7.7).   This
suggests that wastewater dissolution of soil  calcium carbonate is increasing
the pH of the percolating solution, offsetting the production of  hydrogen
ions from nitrification reactions and organic acid formation.

DISSOLVED SOLIDS

Total Dissolved Solids and Electrical Conductivity

     The total dissolved solids (TDS) concentration of municipal  wastewater
is comprised of several ions including sodium (Na+), calcium (Ca  ^),
magnesium (Mg+2), potassium (K+), chloride (Cl~), sulfate (SO/f2), and
others.  Effluent TDS of several primary treatment plants in California range
from 935 to 1,898 mg/L [84].  The secondary effluent at Flushing  Meadows
ranged from 1,000 to 1,200 mg/L [69].

     TDS concentrations can vary widely depending on the industrial  mix of
the community, the proportion of commercial to residential  development, ana
the nature of the residential  community.  Wide variations can be  encountered
from drainage areas having selected land development characteristics yet
having the same water supply [84].

     The effluent and shallow groundwater TDS concentrations at Hoi lister
were both generally in the 1,200 to 1,300 mg/L range.  The salt concentration
of the renovated water is approximately equal to that of the applied
effluent.  Electrical conductivity (EC) measurements bear this out.   TDS and
EC measurements in the intermediate and deep observation wells were highly
variable.  Constrasts using the t-statistic revealed that TDS and EC
measurements in the effluent,  shallow groundwater, and offsite control  wells
were generally indistinguishable.

Exchangeable Cations and Sodium Adsorption Ratio

     Though all four of the major cations (Na+, K+, Ca+ , Mg+ ) found in+
wastewater undergo exchange reactions with soils, the fate of applied Na  is
generally of greatest concern.  When the wastewater ratio of sodium to
calcium and magnesium is large, highly hydrated sodium cations replace less
hydrated Ca and Mg on the soil.  This process causes the dispersion of soil
clay particles, resulting in a decrease in soil permeability.  The nature of
rapid infiltration systems is such that the sodium hazard is generally
considered minimal, because the clay percentage is usually so small  that the
effect is not noticed.

     If the reuse of renovated water for irrigation purposes is an objective,
sodium hazard must be considered.  In most cases soil permeability becomes a
hazard before direct sodium toxicity is noticed.  In a few plants this is not
strictly true, notably avocados [52].  To determine the sodium hazard,  the
sodium adsorption ratio (SAR)  was developed by the U.S. Department of
Agriculture Salinity Laboratory.  SAR is defined as follows:

                         SAR = Na/[l/2 (Ca + Mg)] 1/2

where Na, Ca, and Mg are concentrations of the respective ions in meq/L of

                                     100

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water.  Ratios less than 4 are generally acceptable on almost all  soils
though higher values are suitable for water of higher conductivity   A '
detailed description of the SAR and sodium hazard is found in reference  [12].

     At Hoi lister, concentrations of sodium and potassium in  the  shallow
aquifer were not statistically different (
-------
human consumption.  Sulfates are also important, primarily in industrial
reuse applications, because of their tendency to form scales in boilers  and
heat exchangers.

     The sulfate concentration in the effluent and shallow groundwater at
Hoi lister was less than that contained in the majority of potable water  wells
serving the City of Hoi lister.  The sulfate concentration of Well 3A was
noticeably less than the effluent, while Well 5A was somewhat higher than the
effluent sulfate measured.  An analysis-of-variance reveals that the sulfate
levels in the effluent, treatment site aquifers, and off site control  wells
are indistinguishable (p= 0.78).  Sulfate contamination resulting from
wastewater application has not occurred.

SUSPENDED SOLIDS

     The suspended solids (SS) concentration of untreated municipal
wastewater can range from 100 to 350 mg/L [85].  Municipal effluent from
several primary treatment plants indicates that the suspended solids are
completely removed in most cases [85], if the facility is not overloaded.
The SS in the primary effluent at Hoi lister averages 274 mg/L, indicating the
lack of treatment efficiency by the pretreatment facility.

     The average removal  indicated by total  SS levels in Wells 3A and 5A was
95%.  Effective filtering of input solids occurred although complete  removal
was not indicated after percolation through 7.7 m (22 ft)  of soil.   Improper
well development is probably the cause of inorganic SS levels, which  were
also detected in several  intermediate and deep observation wells.

     Complete evaluation of removal  effectiveness cannot be made because SS
levels in the offsite control  wells were not measured.  However, the  general
appearance of these samples indicated that no SS were present.

ALKALINITY

     Bicarbonate accounted for all of the alkalinity in the Hollister public
water supply (see Table 4) with additional pickup during domestic water  use.
Biological activity, during wastewater percolation through the soil  profile,
can produce carbon dioxide and organic acids.  The resulting action of C02
solubilizes soil carbonate materials and can cause an additional increase in
the alkalinity of the percolate.

     Results at Lake George Village, New York, showed seasonal increases in
alkalinity in shallow observation wells during the summer months [88].  The
well was located 5 m (16 ft) below the surface.  Natural dilution and
biological slowdown are given as reasons for decreases in alkalinity during
winter months [88].

     Alkalinity in the primary effluent at Hollister averaged 446 mg/L,  which
is roughly 110 mg/L greater than the domestic water supply.  In the shallow
observation wells, alkalinity increases were observed relative to input
levels, though the increases were not significant.  The simultaneous
                                      102

-------
 reduction  in  BOD  during percolation implies considerable biological  activity
 and CO-  production.  The resulting attack on soil carbonate material  would
 tend to  increase  alkalinity.

      Alkalinity measurements from the intermediate and deep observation wells
 do not indicate any influence from application of wastewater to the soil
 system.  In  fact,  all measurements are statistically indistinguishable.

 BORON

      The primary  environmental  concern with boron in renovated watPr  ic -itc
 potential toxicity to plants.  Accumulation of excessive boron In^lal?
 tissue results in reduced production   Toxicitv iJmJJc *   k   1n.Plant
                                               c
concentration in domestic wastewater significantly   Bouwer S %i   roi  *   A

mineral pickup during domestic and commercial  use, wastewater boron
concentrations are twice that of the raw water supply, averaging 1.4 mg/L.

     Very few results on boron removal  at rapid infiltration sites have been
reported.  Bouwer et al_. [69] found essentially no boron riSSvI  In the sandJ
and grave ly soils below infiltration basins at Flushing Meadows   The lack
perco^^Wm1^^
concentrations, only 14% of the applied boron is retained in the ove??vina
soil.  The absence of a significant amount of clay is believed to be the
reason for low boron removal.                                        tne
           ^	   x,,,,v.i                                           wells  are

FLUORIDE

     Fluoride is significant in public drinking water supplies  due to  it,
well recognized effects on human teeth [90].   Evidence from nuhiir h   iH
studies has shown that fluoride-ion concentration?^? aPp™ximaie?y fo  mo/.
are optimum for the prevention of dental  fluorosis  and cavities [90]     9

                                     irrigation water.  A maximum  limit  of
                                      Academy  of Science and National  Academy
                                    103

-------
of Engineering for continuous use on all soils [66].   Applications  of soluble
fluoride salts to acid soils can produce toxicity to  plants [91].   Soil  type,
calcium and phosphorus content, and pH seem to be the major factors
controlling plant uptake of fluoride [92].   The adsorption of wastewater
fluoride by soil and its subsequent equilibration with fluorite (CaF2) and
fluorapatite (Cas [P04]3 F) leads to both retention of fluoride in  the soil
and the control of ion-specific injury to plants.  The Ca"1^ added with
wastewater generally maintains the soil Ca  level  high enough to prevent
fluoride injury.  Injury from added NaF has been  demonstrated in acidic soils
low in Ca, but not in well-limed soils [93].  Crops raised on fluoride-
enriched soils show little increased fluoride  uptake  as long as the soil is
near neutral pH.

     Very little information on the fate of fluoride  at operating  rapid
infiltration systems is available.  At Flushing Meadows, Arizona, the
fluoride content of secondary effluent was  reduced from 4.1 to 2.6  mg/L after
movement through 9 m (30 ft) of sandy soil  in  a high  rate application system.
Increased removal was noted with further movement through the coarse textured
soil [69].  The fluoride removal somewhat paralleled  the phosphate  removal
suggesting precipitation of fluorapatite.

     At Hollister, apparently no fluoride is added to the municipal
wastewater from domestic or other water use since concentrations in potable
water  and primary effluent are relatively equal.   The fluoride concentrations
in Wells 3A and 5A increased after 7 m (22 ft) of movement through  the soil
profile.  This  suggests that F~ is currently passing  through the soil profile
without treatment, causing concentration levels to rise relative to baseline
levels.  It may also be possible that the decrease in soil pH resulting from
wastewater addition may cause the dissolution of any  fluorapatite species
present in the  soil profile.

     It should  be noted that even the average fluoride concentration of 1.0
mg/L in Well 3A is below the irrigation water limit set for use on all soils
[66].

TRACE  ELEMENTS

     The greatest concern regarding trace elements has been their potential
health and plant toxicity hazard [68].  Soil has been shown to be effective
in reducing the concentration of trace elements in percolating effluent over
limited periods of time.  However, their long term ability to remove metals
is questioned because of their ineffectiveness after sorption saturation
[60].  This concern would be especially true if the creation of new soil
sorption sites  (influx of wastewater clays and organic material) did not keep
pace with the influx of metals.

     Regulations regarding the recommended maximum concentrations of trace
elements in irrigation waters and maximum allowable concentrations of trace
elements in drinking water have been established.  These  are tabulated  in
Table  30, together with the average effluent concentrations of the Hollister
wastewater.  Except for lead, all average effluent concentrations of trace
elements are below both of these limits.  Effluent lead slightly exceeds
drinking water  standards.

                                     104

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                TABLE 30.  COMPARISON OF TRACE ELEMENT LEVELS
                   TO IRRIGATION AND DRINKING WATER LIMITS
                                     mg/L
Maximum
Recommended maximum concentration
in irrigation in drinking
Element waters [66] waters [94]
Ag (silver)
As (arsenic)
Ba (barium)
Cd (cadmium)
Co (cobalt)
Cr (chromium)
Cu (copper)
Fe (iron)
Hg (mercury)
Mn (manganese)
Ni (nickel)
Pb (lead)
Se (selenium)
Zn (zinc)
__a
0.1
__a
0.01
0.1
0.05
0.2
5.0
__a
0.2
0.2
5.0
0.02
2.0
0.05
0.05
1.0
0.010
~a
0.05
~a
--a
0.002
— a
—a
0.05
0.01
--a
Average
wastewater
concentration
<0.008
<0.01
<0.13
<0.004
<0.008
<0.014
0.034
0.39
<0.001
0.070
0.051
0.054
<0.001
0.048
Average
shallow
groundwater
concentration
<0.006
<0.01
<0. 13
0.028
0.010
O.014
0.038
0.36
<0.001
0.96
0.13
0.09
<0.001
0.081
      a.  None set.


     Most studies investigating solution heavy metal-solid  phase  interactions
have found that specify adsorption,  interfacial  precipitation  bulk
precipitation, and cation exchange can reduce influent heavy metal
concentrations.  Mobility potentials  of heavy metals  are  increased  in acidic
soils, having low ion-exchange capacities and organic matter.

     Several  studies have reported on the fate of trace metals at ooeratina
rapid infiltration projects.   Metal concentrations in the secondary^??lent
and in the renovated water from a well  27 m (86 ft) from  the basing of th«
Flushing Meadows project showed considerable removal  o? copper and  z?nc  but
?f f!f CfdTm 3n? I6ad C69L   Metals d1d not ^cumulate  in the surf ace'l 5m
(5 ft) of the sen   because of  the low organic matter  and  clay content of the
basin soils  the low retention times  of the water in  the  surface  Mils  and
the low metal concentrations in the effluent.            ^rrace  soils, and

     A study  of metal  accumulation in infiltration basins at Fn^t n*»«nf
Massachusetts, revealed a peak of heavy metals wMch  ci?nclSed witS an  '
organic matter accumulation zone at 45  cm (18 in.).   The  SrganlS ™tXr in
this zone and its  metal  content appeared to increase  during winter
decrease during summer [95].                              9 wirrter
                                   105

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The soils at Hollister are low in organic matter with a cation  exchange
capacity typical  of sandy and gravelly soils.   The soil  pH is generally
alkaline favoring heavy metal sorption.

     Of the 14 trace elements monitored in the Hollister groundwater,  five
showed no significant increases in groundwater concentrations relative to
offsite control wells; three  had concentrations that averaged  below the
limits of detection; and six showed significant increases in at least  one
aquifer relative to background levels in the control  wells.

     Groundwater concentrations of Ag, Ba, Cd, Co, and Cr in all  three
aquifers below the treatment site have been unaffected by wastewater
addition.  No statistical differences were observed between treatment  site
aquifers and offsite control wells.  Concentrations,  of Ag, Ba, Cd, Co, and
Cr in the offsite control wells are not significantly different from
concentrations in the primary effluent.

     The F ratio for Cd  is nearly significant (p = 0.11).  This suggests  that
the shallow aquifer may  be exhibiting the first signs of Cd "breakthrough."
The average concentration of Cd in the shallow wells exceeds maximum
allowable concentrations in drinking water, but because of its  variability,
is not  significantly different from the offsite control site wells.  There  is
no soil evidence that suggests Cd mobilization through the upper 300 cm  of
soil (see Figure 41).

     Analyses-of-variance could not be performed for As, Hg, and Se  because
all reported values were below the limit of flame atomic absorption
detection.  While no conclusive statement regarding the transport and  removal
of these elements through the soil profile can be made, it is apparent that
groundwater contamination by these metals is not occurring.

     Treatment site aquifer  concentrations of Mn, Ni, Fe, Zn, Pb, and  Cu  were
significantly  different  than control well values.  Significant shallow
groundwater-control well contrasts were observed for all six metals.   Average
control well values of 0.006, 0.041, 0.003, 0.021, 0.016 and 0.015 mg/L  were
observed for Mn, Ni, Fe, Zn, Pb, and Cu, respectively.  This compares  with
average treatment site shallow groundwater values of 0.961, 0.125, 0.356,
0.081, 0.074, and 0.038  mg/L for the same metals.  Intermediate well-control
well contrasts were observed for all of these metals except Ni.  Deep  well-
control well contrasts were  significant for Cu, Mg, Pb, and Zn.

     Average shallow and intermediate  groundwater concentrations of Pb
exceeded those limits set by the EPA for drinking water.  The concentration
of Mn  in the same groundwaters exceeded the recommended maximum concentration
for irrigation waters used  continuously on all  soils.

     Metal concentrations in some wells were greater than  that input by
wastewater.  This is particularly apparent for  Mn.   Input Mn concentrations
averaged 0.070 mg/L, as  compared to average values of 0.961, 0.357,  0.154
mg/L for shallow^ intermediate, and deep groundwater, respectively.   Lehman
and Wilson [60]  observed similar behavior in a  continuously flooded,
lysimeter-equipped  soil  column.   In their study, more  solution Mn was


                                    106

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 observed  in  soil-water at depths of 15 and 23 cm (6 and 9 in.), than at 8 cm
 (3  in.).   They concluded that Mn originated from within the soil profile   In
 addition,  Ng  and Bloomfield [61, 96] studied trace metal mobilization in soil
 media  and  found that Mn was mobilized by continuous flooding in the presence
 of  organic matter.   Mn mobilization can occur if pH-Eh conditions are
 modified  to  favor the conversions of tetravalent, insoluble manganic forms to
 divalent,  soluble manganous species.   Hem [35] has shown that the solubility
 of  Mnr<1 is enhanced when both pH and Eh are lowered.  The solubility of iron
 also increases when conditions become more acidic and more anaerobic zones
 are created..

     At Hollister, a significant decrease in soil pH was observed after
 wastewater addition.  In addition, the input of an organic rich wastewater
 can create anaerobic environments in the soil.  Immediately after flooding
 the soil surface becomes saturated; oxygen transfer is diminished- and a
 decrease in redox potential  results.  Both the lowered pH and Eh conditions
 favor the solubility of soil  Mn and Fe.   This is substantiated by the
 significant loss of soil  Mn from the upper 30 cm of soil.   Significant
 deposition was not detected through 300  cm,  suggesting that Mn is travelling
 to the underlying groundwater.   In contrast,  soil  Fe showed significant
 increases throughout the soil  profile.   However,  the reduction of ferrous
 iron to ferric, followed by re-oxidation (after drying)  to ferric and
 subsequent precipitation as a hydrous oxide,  could lead  to a less crystalline
 oxide,  more amenable to DTPA  extraction.   Therefore, mobilization of soil  Fe
 cannot be ruled out.

     In addition to the mobilization of  Mn and Fe,  the possibility exists
 that the wastewater addition  has resulted in  the  mobilization of soil  Ni   Zn
 Pb  and Cu.  Ng and Bloomfield [61, 96]  noted that these metals were
mobilized by continuous flooding in the  presence  of organic matter   While
 the formation of metal  chelates may be  an important factor in the transport
of these metals,  the more likely possibility  is that mobilization has
occurred in response to the dissolution  of iron and manganese oxides   It has
often been suggested that these oxides  furnish the  principal  control  on the
fixation of heavy  metals  in soils.   Consequently,  their  loss from the soil
profile could decrease  the  ability of the soil  to  retain input heavy
and dissolve existing sorbed  metals.
                                       s
                                       s,
107

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 2.        Sullivan, R.  H.,  et  al.  Survey of Facilities Using Land
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 3.        Soil  Survey,  San  Benito County, California.  U. S. Department of
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 6.        Climates of the States.  Volume II.   Western States.  U. S.
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10.        Enfield, C. G., and B.  E.  Bledsoe.  Kinetic Model for
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11.        Black, C. A. (ed.).   Methods of Soil  Analysis, Parts 1  and  2.
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12.        Diagnosis and Improvement  of Saline and Alkali Soils.   Agriculture
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13.        Lindsay, W. L. and  W. A. Norvell.  New DTPA-TEA  Soil Test  for Zn
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14.       Kilburn, C.   Groundwater Hydrology  of  the  Hoi lister and San Juan
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15.       Faye, R.E.   Mathematical  Model  of the  San  Juan  Valley  Groundwater
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17.       Buckman, H.  0.  and N.  C. Brady.  The Nature  and Properties of
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18.       Bailey, G.  W.   Role of Soils and Sediment in Water Pollution
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19.       Taylor, S.  A.  and G. L. Ashcroft.   Physical  Edaphology, W. H.
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20.       Haise, H.  R. et a! .  The Use of Cylinder Infil trometers to
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21.       Bouwer, H.   A Study of Final  Infil trati on Rates From  Cyl inder
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24.       Chapman, H.  D.   Cation Exchange Capacity.   In:   Methods  of
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                                                     d-   Stat1st1cs
                                    109

-------
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-------
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-------
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-------
94.       Federal  Register EPA National  Interim Primary Drinking Water
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95.       Schaub.  S.  A.  et al.  Land  Application  of  Wastewater.   Fate of
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          16, 108-135.   1962.
                                     115

-------
TABLE A-l.  WASTEWATER QUALITY RESULTS
      mg/L Unless Otherwise Noted
Parameter
COO
BOO
TOC
Total Kjeldahl
Nitrogen
NH3-h
N-Organ1c
H03-N
Total P
P04-P
Total Conforms,
count/100 ml
Fecal Collforns,
count/100 ml
pH. units
TOS
Conductivity.
mhos/on
SS
Alkalinity
1
F
Na
Ca
Kg
K
Cl
50«
*9
As
Ba
U
Co
Cr
CM
Ft
Hg
Hn
N1
Pb
Se
In
6/21/76
680
ISO
—
46.2
24.4
21.8
0.68
19.5
13.2
1.3 x 106
--
7.0
1,256
1.890
—
—
1.3
0.79
253
56
60
11.7
260
161
0.008
<0.01
<0.1
0.004
0.008
<0.004
0.024
0.34
<0.001
0.056
0.06S
0.012
<0.001
0.016
6/23/76
546
151
—
38.9
22.4
16.50
0.64
22.0
12.8
5.4 x 106
--
7.0
1.184
1.790
—
—
1.0
0.50
244
64
61
11.3
266
180
0.008
<0.01
<0.1
0.005
0.008
<0.004
0.028
0.50
<0.001
0.058
0.065
0.025
<0.001
0.020
6/24/76
647
219
—
39.5
19.7
19.8
0.76
24.0
12.0
49,000
--
7.0
1.152
1,860
—
—
1.1
0.45
242
S3
64
10.9
248
186
0.006
<0.01
<0.1
0.004
0.007
<0.004
0.019
0.38
<0.001
0.094
0.060
0.012
<0.001
0.010
9/15/76
572
211
—
35.3
23.0
12.3
0.17
10
9
46 x 106
11 x ID*
7.2
1.500
2,320
—
—
1.8
0.55
363
70
76
11.7
434
218
0.008
<0.01
<0.1
<0.001
0.014
<0.004
0.031
0.15
<0.001
0.070
0.092
0.045
<0.001
0.035
9/16/76
625
214
..
58.3
44.0
14.3
0.19
12
9
4.6 x 106
4.6 x 106
7.3
1,593
2,400
—
—
1.8
0.6
377
71
69
12.5
488
228
< 0.005
<0.01
<0.1
<0.001
0.009
<0.004
0.021
0.22
<0.001
0.067
0.087
0.033
<0.001
0.030
9/17/76
581
202
-_
45.6
33.6
12.0
0.67
10
9
4.6 x 106
4.6 x 106
7.3
1,585
2,480
..
—
1.7
0.55
391
71
27
12.1
490
210
< 0.005
<0.01
<0.1
<0.001
0.010
<0.004
0.020
0.14
<0.001
O.OS5
0.080
0.022
<0.001
0.030
12/6/76
656
134
„
30.8
21.7
9.1
0.46
21.5
8.5
92 x 106
9.2 x 10^
7.5
1,032
1,600
234
447
1.2
0.37
230
55
74
12.5
185
250
0.010
<0.01
0.11
0.007
<0.006
0.012
0.070
0.35
<0.001
0.067
0.016
0.070
<0.001
0.052
12/7/77
722
145
..
29.2
22.5
6.7
0.44
11.3
11.3
92 x 106
2.2 x 10*
7.8
1,042
1,640
222
437
1.6
0.41
230
52
75
12.9
200
228
0.011
<0.01
0.10
0.007
<0.006
0.018
0.070
0.32
<0.001
0.067
0.015
0.068
<0.001
0.051
12/8/76
598
180
--
31.2
22.8
8.4
0.52
IS. 9
12.2
24 x 106
3.5 x Ifl6
8.1
1,024
1,620
206
416
1.4
0.33
230
53
75
12.5
190
250
0.012
<0.01
0.12
0.008
<0.006
0.015
0.065
0.34
<0.001
0.071
0.015
0.065
<0.001
0.057
2/22/76
840
414
240
40.2
22.6
17.6
0.2
10.0
9.2
16 x 106
16 x 106
7.3
1,072
1,230
328
454
1.4
1.1
196
32
62
16.0
220
225
0.011
<0.01
0.24
0.003
<0.006
0.030
0.030
0.52
<0.001
0.080
0.048
0.120
<0.001
0.090
2/23/77
969
359
240
40.2
23.9
16.3
0.16
10.0
9.9
24 x 106
16 x 1C6
7.1
1,044
1.330
327
465
1.3
1.1
196
31
62
14.8
205
210
4.005
<0.01
0.22
0.003
<0.006
0.036
0.032
0.82
<0.001
0.084
0.038
0.110
<0.001
0.090
2/24/77
1.029
266
264
41.6
23.0
18.6
0.18
10.5
10.4
24 x 10*
24 x 106
7.1
1,016
1.320
327
459
1.3
1.1
200
31
64
16.0
224
210
O.005
<0.01
0.18
0.002
<0.006
0.031
0.035
0.54
<0.001
0.075
0.033
0.070
<0.001
0.080

-------
                                     TABLE B-l.  pH
                                           Units
i.
2.
3.
4.

5.
6.
7.
   0-16
  25-35
 95-105
295-3U5


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
8.500
9.200
9.400
9.400
36.500
9.125
4.6S2
(2)
7.800
3.400
8.900
9.100
34.200
8.550
6.786
TREATMENT SITES
(3)
6.300
6.800
7.900
8.300
29.800
7.450
15.047
(4)
6.400
6.500
7.800
8.800
29.500
7.375
34.333
(5)
6.700
7.600
a. ooo
8.600
30.900
7.725
55.285
MEAN
(6)
7.140
7.700
8.400
8.940
8.045
160.900
RSD3
(7)
13.528
14.520
3.460
3.502

                   CONTROL SITE AVERAGE

         0-16             8.150
        25-35             8.800
       35-105             9.150
      295-305             9.250
                                        TREATMENT SITE AVERAGE


                                                 6.467
                                                 6.967
                                                 7.900
                                                 8.733
                                          -5.545880
                                          -3.537971
                                          -6.228411
                                          -3.661871
     GROUPS  OP NONHETEROGENEOUS MEANS.
     DUNCAN'S  MULTIPLE  RANGE  TEST.b
            8.940
            8.400
            7.700
            7.140
                   8.400
            ********
           9.125
           8.550
           7.725
                   8.550

                   7.450
7.375
a.  Relative standard deviation
b.  Numbers on same horizontal line are nonheterogenous.
    column (6); second set refers to Mean line 6.
                                                     First set of numbers refers  to  Mean

-------
                                          TABLE  B-2.
 CALCIUM CARBONATE
Percent
00
1.
2.
3.
4.
5.
6.
7.

0-16
25-35
95-105
295-305
TOTAL
MEAN
BSD
0-16
25-35
95-105
295-305
CONTROL SITES
(1) (2)
1.550 1.080
2.270 2.060
2.470 2.270
2. 510 3.040
3.300 3.450
2.200 2.113
20.267 38.202
CONTROL SITE AVERAGE
1.315
2.165
2.370
2.775
TREATMENT SITES
(3) (4) (5)
0.230 0.360 0.000
0.270 0.290 0.000
1.170 2.010 0.000
1.420 1.390 2.320
3.140 1.050 2.320
0.785 1.013 0.580
76.139 227.059 427.675
TREATMENT SITE AVERAGE
0.213
0.187
1.060
1.710
MEAN
(6)
0.654
0.978
1.584
2.136
1.338
26.760
"t"
-4.900730
-13.750675
-1.732522
-2.416334
RSDa
(7)
97.857
111.671
64.052
33.598


GROUPS OF NONHETEROGENEOUS MEANS.
DUNCAN'S MULTIPLE RANGE TEST."

2.
1.
0.
136 1.534
534 0.978
978 0.654



* *******

2.
2.
1.
200 2.113
113
013 0.705 0.580



              a.  Relative standard deviation
              b.  Numbers on same horizontal  line are  nonheterogenous.
                  column (6);  second set refers  to Mean  line  6.
                 First set of numbers refers to Mean

-------
                      TABLE B-3.   ELECTRICAL CONDUCTIVITY SOIL  EXTRACT
                                           mmhos/cm
 i.
 2.
 3.
 4.

 5.
 6.
 7.
   0-16
  25-35
 95-105
295-305
  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.740
0.100
0.100
0.400

1.340
0.335
90.984
(2)
0.770
0. 500
0.330
0.500

2.100
0.525
34.654
TREATMENT SITES
(3)
2.943
2.400
1.800
0.700

7.843
1.961
49.034
(4)
2.623
1.230
1.260
0.830

6.043
1.511
110.773
(5)
2.467
0.770
0.500
0.600

4.337
1.084
99.628
MEAN
(6)
1.909
1.010
0.738
0.616
1.083
21. 663


RSDa
(7)
55.906
87.d74
88.086
30.057




                   CONTROL SITE AVERAGE

        0-16             0.755
       25-35             0.300
      95-105             0.215
     295-305             0.450
                                        TREATMENT SITE AVERAGE

                                                2.678
                                                1.483
                                                1.187
                                                0.727
10.609333
 1.851530
 1.965833
 2.467282
     GROUPS OF NONHETEROGENEOUS MEANS.
     DUNCAN'S MULTIPLE RANGE TEST.b
            1.909
            1.010
                        0.798
                                    0.616
            1.961
            1.511
            1.084
            0.525
                  1.511
                  1.084
                  0.525
                  0.335
a.  Relative standard deviation
b.  Numbers on same horizontal line are nonheterogenous.
    column (6); second set refers to Mean line 6.
                                                    First  set of numbers refers to Mean

-------
                                              TABLE B-4.   ORGANIC MATTER
                                                         Percent
             i.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305
  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.76U
0.080
0.020
0.010

0.870
0.218
166.890
(2)
1.350
0.150
0.020
0.020

1.540
0. 385
167.856
TREATMENT SITES
(3)
1.200
0.200
0.060
0.110

1.570
0.393
137.947
(4)
2.190
0.120
0.030
0.080

2.420
0.605
89.501
(5)
2.020
0.280
0.050
0.030

2.380
0.595
63.607
MEAN
(6)
1.504
0.166
0.036
0.050
0.439
3.780


RSD3
(7)
39.427
46.585
50.461
86.023




ro
o
              CONTROL SITE AVERAGE

    0-16             1.055
   25-35             0.115
  95-105             0.020
 295-305             0.015
         TREATMENT SITE AVERAGE

                 1.803
                 0.200
                 0.047
                 0.073
                                                                                              't"
                   1.656706
                   1.305976
                   2.342160
                   1.921840
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST."
                         1.504
                         0.166
                   0.050
0.036
                          ********

                         0.605       0.595
                               0.393
            0.385
0.218
              a.  Relative standard deviation
              b.  Numbers on same horizontal line are nonheterogenous.
                  column (6); second set refers to Mean line 6.
                                                      First set of numbers refers to Mean

-------
                                               TABLE  B-5.
                                          TOTAL  NITROGEN
                                          ppm
                                  CONTROL SITES
                                                               TREATMENT SITES
                                                                                             MEAN
                                                                                       RSD°

1.
2.
3.
4.

5.
6.
7.

0-16
25-35
95-105
295-305

TOTAL
MEAN
RSO
(1)
559.000
76.000
64.000
45.000

744.000
186.000
133.868
(2)
840. 000
112.000
34.000
184.000

1170.000
292.500
126.531
(3)
1182.000
372.000
117.000
178.000

1849.000
462.250
106/435
(4)
1722.000
347.000
93.000
187.000

2349.000
587.250
100.985
(5)
1586.000
200.000
104.000
77.000

1967.000
491.750
54.328
(6)
1177.800
221.400
82.400
134.200
403.950
8079.000

(7)
41.590
60.608
40.511
50.559



ro
              CONTROL SITE AVERAGE

    0-16            699.500
   25-35             94.000
  95-105             49.000
 295-305            114.500


GROUPS OF NONHETEROGENEOUS MEANS.
DUNCAN'S MULTIPLE RANGE TEST.*3
            TREATMENT SITE AVERAGE

                   1496.667
                    306.333
                    104.667
                    147.333
                                                                                              •t"
                                                                                            3.405576
                                                                                            3.009486
                                                                                            3.886174
                                                                                            0.476101
                      1177.800
                       221.400
                                   134.200
                                                82.400
                          ********
                       587.250
                       491.750
                 491.750
                 462.250
462.250
292.500
292.500
186.000
             a.  Relative standard deviation
             b.  Numbers on same horizontal line are nonheterogenous.
                 column (6); second set refers to Mean line 6.
                                                     First set of numbers refers to Mean

-------
                                              TABLE B-6.
                                        ORGANIC  NITROGEN
                                         ppm
             i.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305

 TOTAL
  MEAN
   RSO
CONTROL
(1)
545.000
62.000
45.000
34.000
686.000
171.500
145.345
SITES
(2)
818.000
98.000
30.000
173.000
1119.000
279.750
129.957
TREATMENT SITES
(3)
1122.000
326.000
99.000
137.000
1684.000
421.000
113.482
(4)
1592.000
290.000
74.000
150.000
2106.000
526.500
98.373
(5)
1491.000
174.000
86.000
65.000
1816.000
454.000
43.108
MEAN
(6)
1113.600
190.000
66.800
111.800
370.550
7411.000
RSDa
(7)
39.706
60.925
42.912
53.073

ro
ro
             CONTROL SITE AVERAGE

   0-16           681.500
  25-35            80.000
 95-105            37.500
295-305           103.500
           TREATMENT SITE AVERAGE

                 1401.667
                  263.333
                    86.333
                  117.333
                                                                                             •t"
                               3.419416
                               3.020034
                               4.493534
                               0.222999
                  GROUPS OF NONHETEROGENEOUS  MEANS.
                  DUNCAN'S MULTIPLE  RANGE TEST.b
                      1113.600
                       190.000
                111.800
                                                66.800
                          * *******
                       526.500
                       454.000
                454.000
                421.000
421.000
279.750
279 .750
171.500
             a.   Relative standard deviation
             b.   Numbers on  same  horizontal line  are  nonheterogenous.
                  column  (6);  second  set  refers  to Mean line 6.
                                                     First set of numbers refers to Mean

-------
                                             TABLE  B-7.
                                        TOTAL PHOSPHORUS
                                         ppm
             i.
             2.
             3.
             4.

             5.
             6.
             7.
ro
CO
   0-16
  25-35
 95-105
295-305


 TOTAL
  MEAN
   RSO
CONTROL SITES
(1)
705
609
385
334

2033
508
34
.UOO
.000
.000
.QUO

.000
.250
.905
CONTROL




748
532
374
401
(2)
792.
456.
363.
468.

2079.
519.
36.
000
000
000
000

000
750
069
TREATMENT SITES
(3)
1560
1192
719
964

4435
HOB
32
SITE AVERAGE
.500
.500
.000
.000








.000
.000
.000
.000

.000
.750
.243
(4)
1965.
1440.
788.
842.

5035.
1258.
104.
000
000
000
000

000
750
694
(5)
2820
1403
923
850

5996
1499
107
.000
.000
.000
.000

.000
.000
.582
TREATMENT SITE AVERAGE




2115.
1345.
810.
885.
000
000
000
333








MEAN
(6)
1568 .400
1020.000
635.600
691.600
97U.900
19578.000


"t"
2.843629
7.073207
5.605943
6.794494
RSDa
(7)
55.877
44.919
39.324
39.584









   0-16
  25-35
 95-105
295-305
                  GROUPS  OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S  MULTIPLE  RANGE TEST.b
                      1568.400
                      1020.000
                691.600
 635.600
                          ********
                      1499.000
                      1258.750
                      1108.750
                       519.750
               1258.750
               1108.750

                508.250
1108.750
             a.  Relative standard deviation
             b.  Numbers on same horizontal line are nonheterogenous.
                 column (6); second set refers to Mean line 6.
                                                     First set of numbers refers to Mean

-------
                                         TABLE  B-8.  BICARBONATE  EXTRACTABLE
                                                     PHOSPHORUS, ppm
             i.
             2.
             3.
             4.


             5.
             6.
             7.
    0-16
   25-35
 95-105
 295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
13.000
0.670
0.560
3.700

17.930
4.483
130.770
(2)
8.400
0.150
0.090
0.170

8.810
2.203
187.596
TREATMENT SITES
(3)
57.000
61.000
42.000
54.000

214.000
53.500
15.300
(4)
109.000
73.000
39.000
32.000

253.000
63.250
114.806
(5)
111.000
63.000
33.000
21.000

228.000
57.000
115.156
MEAN
(6)
59.680
39.564
22.930
22.174
36.087
721.740


RSD3
(7)
83.285
91.070
91.099
99.223




ro
              CONTROL SITE AVERAGE

    0-16             10.700
   25-35              0.410
  95-105              0.325
 295-305              1.935

GROUPS OF NONHETEROGENEOUS MEANS.
DUNCAN'S MULTIPLE RANGE TEST."
                        59.680
                        39.564
                  39.564
                  22.930
          TREATMENT SITE AVERAGE


                    92.333
                    65.667
                    38.000
                    35.667
                                                                                             •t"
                                                                                           3.567259
                                                                                          13.606802
                                                                                          11.015636
                                                                                           2.678614
22.174
                          ********
                        63.250
                        57.000
                        53.500
                         4.483
                  57.000
                  53.500

                   2.203
53.500
              a.   Relative standard deviation
              b.   Numbers on same horizontal line are nonheterogenous.
                  column (6); second set refers to Mean line 6.
                                                      First set of numbers refers to Mean

-------
                                                    TABLE B-9.  BORON
                                                            ppm
              1.
              2.
              3.
              4.

              5.
              6.
              7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.190
0.170
0.140
0.130

0.630
0.158
17.484
(2)
0.220
0.150
0.090
0.170

0.630
0.158
34.142
TREATMENT SITES
(3)
0.660
0.360
0.180
0.380

1.580
0.395
50.188
(4)
1.080
0.350
0.170
0.470

2.070
0.518
109.863
(5)
0.700
0.340
0.160
0.200

1.400
0.350
101.967
MEAN
(6)
0.570
0.274
0.148
0.270
0.316
6.310


RSDa
(7)
65.173
38.156
24.079
54.496




ro
en
   0-16
  25-35
 95-105
295-305
                                CONTROL SITE AVERAGE
0.205
0.160
0.115
0.150
                                                          TREATMENT SITE AVERAGE
0.813
0.350
0.170
0.350
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
 3.513570
18.024983
 2.740501
 1.931468
                         0.570
                         0.274
                                     0.270
                                                 0.143
                         ****** **
                        0.518
                        0.395
                  0.395
                  0.350
          0.350
          0.158
                                                             0.158
             a.  Relative standard deviation
             b.  Numbers on  same horizontal line  are  nonheterogenous.
                 column  (6); second set refers  to Mean  line  6.
                                                     First set of  numbers  refers  to  Mean

-------
ro
             1.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305

  TOTAL
   MEAN
     RSD
                                       TABLE B-10.   CATION EXCHANGE CAPACITY
                                                      meq/100 g
CONTROL SITES

7
2
1
2

14
3
53
(1)
.170
.790
.910
.310

.180
.545
.025
CONTROL




7
3
1
2

8
3
1
2

15
3
63
SITE
.905
.020
.575
.445
(2)
.640
.250
.240
.580

.710
.928
.681
AVERAGE





4
4
3
6

19
4
25
TREATMENT SITES
(3)
.820
.140
.190
.930

.080
.770
.606
(4)
11.170
6.210
2.700
7.500

27.580
6.395
71.152
TREATMENT SITE








8.910
4.837
2.623
5.887

10
4
1
3

20
5
24
AVERAGE




(5)
.740
.160
.930
.230

.110
.028
.511






8
4
2
4
4
MEAN
(6)
.508
.110
.204
.510
.333
RSDa
(7)
30.805
31.945
34.286
55.432

96.660



0
2
2
1


It . II
.372073
.011952
.024490
.988362







    0-16
   25-35
  95-105
 295-305
                  GROUPS  OF  NONHETEROGENEOUS  MEANS.
                  DUNCAN'S MULTIPLE  RANGE  TEST.b
                        8.508
                        4.510
                                    4.110
                                                2.204
                         ********
                        6.895
                        5.028
                   5.028
                   4.770
4.770
3.928
                                                            3.545
             a.
             b.
Relative standard deviation
Numbers on same horizontal line are nonheterogenous.
column (6); second set refers to Mean line 6.
                                                                       First set of numbers refers to Mean

-------
              1.
              2.
              3.
              4.


              5.
              6.
              7.
PO
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
                                                TABLE B-ll.   EXCHANGEABLE
                                                    SODIUM, meq/100 g
CONTROL SITES
(1)
0.930
0.110
0.001
0.120
1.161
0.290
148.111
CONTROL
0
0
0
0
(2)
0.410
0.070
0.020
0.050
0.550
0.138
132.964
SITE AVERAGE
.670
.090
.011
.085
TREATMENT SITES
(3)
1.210
0.770
0.390
0.910
3.280
0.820
41.511
(4)
3.410
0.590
0.220
0.740
4.960
1.240
106.775
(5)
1.230
0.480
0.260
0.140
2.110
0.528
105.954
TREATMENT SITE AVERAGE

1.950
0.613
0.290
0.597

MEAN
(6)
1.438
0.404
0.178
0.392
0.603
12.061
It A. tl
1.330321
4.751761
4.195057
1.690705
RSD3
(7)
80.039
75.517
92.943
102.350


   0-16
  25-35
 95-105
295-305
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
                         1.433
                         0.404
                                     0.392
                              0.178
                          ********
                         1.240
                         0.820
                  0.820
                  0.528
0.528
0.290
                                                            0.138
              a.   Relative standard  deviation
              b.   Numbers on same horizontal  line are  nonheterogenous.
                  column (6);  second set  refers  to Mean  line  6.
                                                    First set of numbers refers to Mean

-------
                                               TABLE B-12.   EXCHANGEABLE
                                                 POTASSIUM,  meq/100 g
             1.
             2.
             3.
             4.


             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.260
0.020
0.001
0.010
0.231
0.073
171.923

0
0
0
0
0
0
162
(2)
.300
.050
.001
.001
.352
.088
.737

0
0
0
0
0
0
46
TREATMENT SITES
(3)
.220
.130
.060
.160
.570
.143
.681

0
0
0
0
0
0
72
(4)
.340
.160
.010
.160
.670
.168
.404

0
0
0
0
0
0
28
(5)
.380
.100
.090
.020
.590
.148
.968
MEAN

0
0
0
0
0
2
(6)
.300
.092
.032
.070
.124
.473
RSDa
(7)
21.082
62. 156
124.926
117.166

00
   0-16
   25-35
 95-105
 295-305
CONTROL SITE AVERAGE


      0.280
      0.035
      0.001
      0.006
TREATMENT SITE AVERAGE


        0.313
        0.130
        0 .053
        0.113
                                                                                             111"
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
0.522233
3.800000
1.737310
1.787104
                         0.300
                         0.092
                    0.070
                 0.032
                          -********
                         0.168
                         0.148
                    0.148
                    0.143
                 0.143
                 0.038
   0.088
   0.073
              a.  Relative standard deviation
              b.  Numbers on same horizontal line are nonheterogenous.
                  column  (6); second set refers to Mean line 6.
                                                       First  set of  numbers  refers  to  Mean

-------
                                                  TABLE B-13.   EXCHANGEABLE
                                                    MAGNESIUM, meq/100 g
              1.
              2.
              3.
              4.

              5.
              6.
              7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
1.840
1.120
1.250
1.480
5.690
1.423
22.189

1
1
0
7
11
2
114
(2)
.810
.140
.700
.590
.240
.810
.562

3
2
2
4
12
3
25
TREATMENT SITES
(3)
.300
.880
.240
.180
.600
.150
.819

6
4
1
4
16
4
108
(4)
.970
.020
.310
.210
.510
.128
.126

6
2
1
1
12
3
95
(5)
.320
.590
.430
.880
.220
.055
.136
MEAN

4
2
1
3
2
58
(6)
.048
.350
.386
.868
.913
.260
RSD3
(7)
60.692
52.568
39.946
62.960

ro
   0-16
  25-35
 95-105
295-305
CONTROL SITE AVERAGE


      1.825
      1.130
      0.975
      4.535
                  GROUPS OF NONHETEROGENEOUS  MEANS.
                  DUNCAN'S MULTIPLE RANGE  TEST."
TREATMENT SITE AVERAGE


        5.530
        3.163
        1.660
        3.423
                                                                                            2.538119
                                                                                            3.608478
                                                                                            1.596132
                                                                                           -0.447265
                          4.043
                                     3.368
                                                 2.350
                                           1.386
                           ********

                          4.128       3.150
                                                  3.055
                                                             2.810
                                                                         1.423
               a.  Relative standard deviation
               b.  Numbers on same horizontal  line are  nonheterogenous.  First set of numbers refers to Mean
                   column (6);  second set refers  to Mean  line 6.

-------
                                                 TABLE B-14.  EXCHANGEABLE
                                                    CALCIUM, meq/100 g
             i.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL
(1)
5.550
3.020
1.750
1.450
11.770
2.943
63.442
SITES

5
1
0
3
11
2
72
(2)
.660
.820
.830
.150
.510
.878
.134

0
0
2
4
7
1
103
TREATMENT SITES
(3)
.640
.010
.790
.510
.950
.988
.658

1
0
2
6
10
2
96
(4)
.670
.090
.310
.240
.310
.578
.381

0
0
0
2
4
1
336
(5)
.940
.180
.570
. 720
.410
.103
.062
MEAN

2
1
1
3
2
45
(6)
.892
.024
.660
.614
.298
.950
RSDa
(7)
86.621
131.297
56.382
50.637

CO
o
   0-16
  25-35
 95-105
295-305
CONTROL SITE AVERAGE


       5.605
       2.420
       1.315
       2.300
TREATMENT SITE AVERAGE

         1.083
         0.093
         1.890
         4.490
                                                                                              •t"
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
-11.390349
 -5.151093
  0.618915
  1.503232
                        3.614
                   2.892
                 1.660
                                           1.024
                         ********

                         2.943        2.878        2.578
                                           1.988
                                         1.103
              a.   Relative standard deviation
              b.   Numbers on same horizontal line are nonheterogenous.
                  column (6); second set refers to Mean line 6.
                                                      First set of numbers refers to Mean

-------
                                TABLE B-15.  DTPA-IRON
                                           ppm
CONTROL SITES

1.
2.
3.
4.
5.
6.
7.

0-16
25-35
95-105
295-305
TOTAL
MEAN
RSO
(1)
19.000
7.560
6.300
7.000
39.860
9.965
60 .666
(2)
23.
6.
3.
7.
41.
10.
32.
000
990
700
990
630
420
386
CONTROL SITE AVERAGE

0-16
25-35
95-105
295-305

21.000
7.275
5.000
7.495

TREATMENT SITES
(3) (4) (5)
122.000 274.
104.000 160.
41.000 53.
26.000 32.
293.000 519.
73.250 129.
64.008 115.
000 169.000
000 140.000
000 28.000
000 14.000
000 351.000
750 67.750
035 114.235
TREATMENT SITE AVERAGE
188
134
40
24
.333
.667
.667
.000
MEAN
(6)
121.400
33.710
26.400
17.398
&2.227
1244.540
"t"
2.883835
6.022371
3.806608
2.412568
RSD3
(7)
88.066
86.732
81.298
63.965


GROUPS OF NONHETEROGENEOUS MEANS.
DUNCAN'S MULTIPLE RANGE TEST.b

121.
83.
400 83
710 26
.710
.400
17.393




********

129.
87.
73.
750 37
750 73
250 10
.750
.250
.420
73.250
10.420
9.965




a.  Relative standard deviation
b.  Numbers on same horizontal  line are  nonheterogenous.
    column (6);  second set refers  to Mean  line  6.
First set of numbers refers to Mean

-------
                                 TABLE B-16.
                                          DTPA-MANGANESE
                                        ppm
i.
2.
3.
4.

5.
6.
7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
5.450
2.520
1.680
2.090

11.740
2.935
58.309
(2)
5.180
2.580
1.230
2.380

11.370
2.843
58.681
TREATMENT SITES
(3)
2.240
1.040
1.290
2.160

6.730
1.683
36.083
(4)
3.140
0.540
2.100
4.360

10.140
2.535
95.380
(5)
2.070
0.660
1.990
4.590

9.310
2.328
108.117
MEAN
(6)
3.616
1.468
1.658
3.116
2.465
49.290


RSD3
(7)
44.420
68.464
23.d35
40.047




                   CONTROL SITE AVERAGE

        0-16              5.315
       25-35              2.550
      35-105              1.455
     295-305              2.235


     GROUPS OP NONHETEROGENEOUS MEANS.
     DUNCAN'S  MULTIPLE RANGE  TEST.b
                                        TREATMENT SITE AVERAGE

                                                  2.483
                                                  0.747
                                                  1.793
                                                  3.703
    •t"
-6.432115
-9.2J8372
 0.919582
 1.459979
           3.616
           3.116
                  3.116
                  1.658
                                   1.468
            ******* *

           2.935
                  2.843
                                   2.535
                                              2.328
                                                           1.683
a.  Relative standard deviation
b.  Numbers on same horizontal line are nonheterogenous.
    column  (6); second set refers to Mean line 6.
                                                     First set of numbers refers to Mean

-------
                                                TABLE B-17.  DTPA-NICKEL
                                                            ppm
             i.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.620
0.240
0.200
0.190

1.250
0.313
65.363
(2)
0.730
0.270
0.140
0.240

1.380
0.345
76.120
TREATMENT SITES
(3)
0.740
0.540
0.500
0.280

2.060
0.515
36.620
(4)
2.430
1.040
0.390
0.320

4.180
1.045
108.406
(5)
1.300
0.330
0.310
0.440

2.380
0.595
83.081
MEAN
(6)
1.164
0.484
0.308
0.294
0.563
11.250


RSD3
(7)
64.937
68.628
46.904
32.232




GO
GO
              CONTROL SITE AVERAGE

   0-16             0.675
  25-35             0.255
 95-105             0.170
295-305             0.215
          TREATMENT SITE AVERAGE

                    1.490
                    0.637
                    0.400
                    0.347
                                                                                             "t"
1.267567
1.402727
3.085774
2.031885
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
                        1.164
                        0.484
                                    0.308
                                                0.294
                         ***** ***
                        1.045
                        0.595
                   0.595
                   0.515
0.515
0.345
                                                            0.313
             a.  Relative standard deviation
             b.  Numbers on same horizontal line are nonheterogenous.
                 column (6); second set refers to Mean line 6.
                                                     First  set of  numbers  refers  to Mean

-------
                                               TABLE  B-18.  DTPA-COBALT
                                                           ppm
CA>
1.
2.
3.
4.
5.
6.
7.

0-16
25-35
95-105
295-305
TOTAL
MEAN
RSD
0-16
25-35
95-105
295-305
CONTROL SITES
(1) (2)
0.080 0.040
0.070 0.010
0.010 0.010
0.010 0.010
0.170 0.070
0.043 0.018
88.822 85.714
CONTROL SITE AVERAGE
0.060
0.040
0.010
0.010
TREATMENT SITES
(3) (4) (5)
0.100 0.140 0.040
0.070 0.050 0.040
0.050 0.030 0.030
0.090 0.040 0.050
0.310 0.260 0.160
0.078 0.065 0.040
28.611 65.271 96.825
TREATMENT SITE AVERAGE
0.093
0.053
0.037
0.060
MEAN
(6)
0.080
0.048
0.026
0.040
0.049
0.970
11 1"
0.825723
0.531369
3.098387
2.535463
RSDa
(7)
53.033
51.875
64.358
82.916


GROUPS OF NONHETEROGENEOUS MEANS.
DUNCAN'S MULTIPLE RANGE TEST.b



0
0

0
0
0
.080 0.048
.048 0.040 0.026
********
.078 0.065 0.043
.065 0.043 0.040
.043 0.040 0.018


0.040






               a.  Relative standard deviation
               b.  Numbers on same horizontal line are nonheterogenous.
                   column (6);  second set refers to Mean line 6.
First set of numbers refers to Mean

-------
                                                  TABLE B-iy.   DTPA-ZINC
                                                            ppm
              i.
              2.
              3.
              4.

              5.
              6.
              7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.980
0.120
0.040
0.050
1.190
0.298
153.408
(2)
1.590
0.210
0.030
0.070
1.900
0.475
157.332
TREATMENT SITES
(3)
10.560
3.420
0.210
0.230
14.420
3.605
135.254
(4)
8.040
1.100
0.240
0.180
9.560
2.390
112.969
(5)
7.520
1.420
0.270
0.320
9.530
2.383
108.717
MEAN
(6)
5.738
1.254
0.158
0.170
1.830
36.600
RSDa
(7)
73.719
106.383
72.357
66.160

CO
in
              CONTROL SITE AVERAGE

   0-16             1.285
  25-35             0.165
 95-105             0.035
295-305             0.060
         TREATMENT  SITE AVERAGE

                    8.707
                    1.980
                    0.240
                    0.243
                               6.018932
                               1.935515
                               9.043140
                               3.433033
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
                         5.738
                         1.254
                                     0.170
                                                 0.158
                          ********
                         3.605
                  2.390
2.383
0.475
0.298
              a.   Relative standard deviation
              b.   Numbers  on same horizontal line are nonheterogenous.   First set of numbers refers to Mean
                  column (6);  second set refers to Mean line 6.

-------
                                          TABLE B-20.   DTPA-CADMIUM
                                                      ppm
             i.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    RSD
CONTROL SITES
(1)
0.080
0.010
0.010
0.010

0.110
0.028
127.273
(2)
0.120
0.020
0.010
0.010

0.160
0.040
133.853
TREATMENT SITES
(3)
0.140
0.070
0.020
0.030

0.260
0.065
83.796
(4)
0.240
0.050
0.010
0.010

0.310
0.078
98.056
(5)
0.180
0.040
0.010
0.010

0.240
0.060
23.570
MEAN
(6)
0.152
0.038
0.012
0.014
0.054
1.080


RSDa
(7)
40.126
62.828
37.268
63.888




en
              CONTROL SITE AVERAGE

   0-16             0.100
  25-35             0.015
 95-105             0.010
295-305             0.010
         TREATMENT SITE AVERAGE

                   0.187
                   0.053
                   0.013
                   0.017
2.146879
3.199798
0.774597
0.774597
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST."
                         0.152
                         0.038
                   0.014
0.012
                          ********
                         0.078
                   0.065
                                                 0.060
                                           0.040
                                                                         0.028
              a.  Relative standard deviation
              b.  Numbers on same horizontal line are nonheterogenous.
                  column  (6); second set refers to Mean  line  6.
                                                      First set of numbers refers to Mean

-------
                                                TABLE  B-21.  DTPA-COPPER
                                                            ppm
              i.
              2.
              3.
              4.


              5.
              6.
              7.
   0-16
  25-35
 95-105
295-305
  TOTAL
   MEAN
    RSO
CONTROL SITES
(1)
2.410
0.350
0.710
0.240
3.710
0.928
108.734
(2)
4.030
0.360
0.170
0.370
4.930
1.233
151.503
TREATMENT SITES
(3)
6.720
2.700
1.490
2.290
13.200
3.300
70.749
(4)
8.230
2.230
0.370
1.190
12.520
3.130
105.472
(5)
6.200
1.440
0.500
0.600
8.740
2.185
42.282
MEAN
(6)
5.518
1.416
0.748
0.938
2.155
43.100
RSDa
(7)
41.661
75.431
65.579
89.442

CO
—I
              CONTROL SITE AVERAGE

    0-16              3.220
   25-35              0.355
  95-105              0.440
 295-305              0.305
          TREATMENT SITE AVERAGE

                 7.050
                 2.123
                 0.953
                 1.360
3.864505
3.725866
1.211560
1.645489
                  GROUPS OF NONHETEROGENEOUS MEANS.
                  DUNCAN'S MULTIPLE RANGE TEST.b
                         5.518
                         1.416
                   0.938
0.748
                          ***** ***
                         3.300
                         3.130
                         2.185
                  3.130
                  2.185
                  1.233
2.185

0.928
              a.   Relative standard deviation
              b.   Numbers on same horizontal line are nonheterogenous.
                  column (6);  second set refers to Mean line 6.
                                                     First set of  numbers refers to Mean

-------
                                               TABLE B-22.  DTPA-LEAD
                                                          ppm
             i.
             2.
             3.
             4.

             5.
             6.
             7.
   0-16
  25-35
 95-105
295-305


  TOTAL
   MEAN
    BSD
CONTROL SITES
(1)
5.080
0.170
0.140
0.120
5.510
1.378
179.196
(2)
10.370
0.360
0.090
0.170
10.990
2.748
185.002
TREATMENT SITES
(3)
3.520
0.590
0.350
0.410
4.870
1.218
126.356
(4)
5.680
0.670
0.290
0.360
7.000
1.750
121.753
(5)
6.580
0.280
0.170
0.170
7.200
1.800
312.206
MEAN
(6)
6.246
0.414
0.208
0.246
1.779
35.570
RSDa
(7)
40.998
50.799
52.048
52.736

CO
CO
              CONTROL SITE AVERAGE

    0-16              7.725
   25-35              0.265
  95-105              0.115
 295-305              0.145

GROUPS OF NONHETEROGENEOUS MEANS.
DUNCAN'S MULTIPLE RANGE TEST.b
                                                          TREATMENT SITE AVERAGE
                 5.260
                 0.513
                 0.270
                 0.313
-1.074722
 1.468735
 2.188993
 1.749809
                        6.246
                        0.414
                  0.246
0.208
                         ********
                        2.748
                   1.800
1.750
                                                            1.378
                                                                        1.218
              a.   Relative  standard deviation
              b.   Numbers on same horizontal Line are nonheterogenous.
                  column (6);  second set refers to Mean line 6.
                                                      First set of .numbers refers to Mean

-------
TABLE C-l.  WELL 3A GROUNDWATER QUALITY RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total collform ,
count/ 100 ml
Fecal collform ,
count/100 ml
pH, units
TDS
Conductivity,
wnhos/cm
SS
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Hn
N1
Pb
Se
Zn
SAR

July
60
3
—
4.1
<0.2
3.8
0.14
6.5
5.6
>2.4 x 105
>2.4 x 105
7.5
1,285
1,620
—
--
1.0
1.5
230
119
57
12.5
258
65
0.006
<0.01
<0.1
0.003
0.008
<0.004
0.030
0.49
<0.001
1.65
0.075
0.009
<0.001
0.066
4.34
1976
Sept
27
<6
—
3.5
1.6
1.0
0.9
7.9
5.8
4.6 x 106
430,000
7.4
1,504
2,220
—
—
1.7
1.4
327
106
78
16.0
402
228
<0.005
<0.01
<0.1
0.010
0.012
<0.004
0.094
0.12
<0.001
0.81
0.115
0.10
<0.001
0.084
5.88

Dec
71
16
--
2.7
0.1
0.9
1.8
11.4
9.4
35,000
35,000
7.7
1.200
1,580
—
521
1.2
0.4
239
82
78
12.1
250
194
0.005
0.01
<0.02
0.010
<0.006
<0.004
0.038
<0.01
<0.001
1.8
0.04
0.11
<0.001
0.021
4.53
Mar
1
39
5
10
2.2
3.7
0
1.1
2.6
7.4
7.4
350.000
24,000
7 c
' . 3
1.138
1,420
..
514
1.0
0.9
219
68
74
11.3
215
168
<0.005
<0.01
0.23
0.003
<0.006
0.032
0.04
0.20
<0.001
0.94
0.16
0.06
<0.001
0.13
4.37
1977
2
32
<2
..
4.9
4.3
0.6
6.6
240.000
49.000

--
15
580
—
..
230
126
70
12.9
..
—
--
-_
..
—
--
—
—
..
..
—
—
—
__
..
4.07
Average
46
6

3.8
<0.4
2.2
1.2
8.0
6.8
1.1 x 106
156,000

7.5
1,282
1,710
..
538
1.2
1.1
249
100
71
13.0
281
164
<0.006
<0.01
<0.11
0.007
<0.008
<0.011
0.041
0.21
O.001
1.30
0.10
0.10
<0.001
0.073
4.64
Standard
deviation
19
5.6

0.8
<0.8
1.7
1.0
2.0
1.8
2.0 x 106
177,000

0.1
160
351

36
0.3
0.5
44
25
9.0
1.8
83
70
<0.001

<0.09
0.004
<0.003
<0.014
0.010
0.21

0.50
0.05
0.06

0.041
0.71
Coefficient
of variation
0.41
0.93

0.21
? n
t . u
0.77
0.83
0.25
0.26
1.82
1.13

0.01
0.12
0.21

0.07
0.25
0.45
0.18
0.25
0.13
0.14
0.3
0.43
0.17

0.82
0.57
0.38
1.27
0.24
1.0

0.38
0.5
0.6

0.56
0.15
                      139

-------
TABLE C-2.  WELL 5A GROUNDWATER QUALITY RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total collform,
count/100 ml
Fecal coHfonn,
count/100 ml
pH, units
TDS
Conductivity,
umhos/cm
SS
Alkalinity
B
F
Na
Ca
Hg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
N1
Pb
Se
Zn
SAR

July
	
10
--
—
<0.2
<0.2
<0.2
0.05
0.13
0.13
54,000
<2
7.9
1,090
1,810
--
—
0.7
0.75
248
133
75
13.6
280
280
0.008
<0.01
<0.1
0.005
0.018
<0.004
0.014
0.55
<0.001
0.70
0.115
<0.005
O.001
0.118
4.26
1976
Sept
56
<6
--
—
1.4
<0.1
<1.3
0.11
19
13
1.1 x 106
930,000
7.6
1,462
2,020
--
—
1.7
--
308
101
70
16.4
342
280
<0.005
<0.01
<0.1
0.085
0.019
<0.004
0.058
0.19
<0.001
0.81
0.115
0.10
<0.001
0.073
5.76
Mar 1977
Dec
78
43
--
—
2.8
<0.1
1.0
1.8
11.2
9.9
<2
0
7.5
1,388
2,000
--
516
1.3
0.4
285
120
85
17.6
315
218
0.003
<0.01
O.02
0.10
<0.006
O.004
0.052
0.04
<0.001
0.8
0.04
0.16
<0.001
0.037
4.86
1
33
5
11
1.7
2.8
0.5
1.4
0.9
10.2
10.2
1,600
1,600
7.7
1,160
1,480
—
519
1.1
1.2
223
71
66
13.3
230
210
<0.005
<0.01
0.38
0.008
0.006
0.055
0.02
1.25
<0.001
0.18
0.38
0.04
<0.001
0.13
4.44
2
32
2
--
—
1.4
--
1.3
0.1
7.3
—
1.300
790
~
—
--
10
530
--
—
242
112
—
«
-.
—
—
--
—
—
..
—
—
—
..
«
..
..
—
..
4.48
Average
50
13
--
—
1.7
<0.2
<1.0
0.6
9.6
8.3
231,000
186,000
7.7
1,275
1,828
~
522
1.2
0.80
261
107
74
14.9
292
247
<0.007
<0.01
<0.15
0.050
<0.012
<0.017
0.036
0.51

-------
TABLE C-3.   WELL IB GROUNDWATER QUALITY  RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOO
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total coif form ,
count/100 ml
Fecal coHform,
count/ 100 ml
pH, units
TOS
Conductivity,
ymhos/cm
SS
Alkalinity
e
F
Na
Ca
Mg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
N1
t>b
Se
Zn
SAR

July
25
3
--
0.72
7.1
<0.2
0.2
6.9
0.06
0.06

<2

0
8.1
846
1,170
—
—
0.5
0.4
115
57
77
3.5
88
210
<0.004
<0.01
<0.1
0.002
0.010
<0.004
0.04
1.25
<0.001
0.40
0.04
0.04
<0.001
0.16
2.33
1976
Sept
10
0
--
-
4.0
<0.1
<0.1
4.0
0.13
0.11

<2

0
7.8
817
1,210
..
—
0.7
0.4
143
30
76
3.5
82
228
<0.005
<0.01
<0.1
<0.0"01
<0.006
<0.004
0.039
0.14
<0.001
0.083
0.055
0.015
<0.001
0.14
3.16

Dec
32
5
—
—
5.4
0.4
0.6
4.4
0.08
0.04

<2

0
8.7
748
1,090
..
341
0.7
0.2
133
57
84
4.7
60
200
0.003
<0.01
0.08
0.008
<0.006
0.01
0.09
0.07
<0.001
0.22
0.02
0.085
--O.C01
0.70
2.62
Mar 1977
18
<2
9
—
10.1
0.0
1.1
9.0
0.3
0.3

<2

0
8.3
804
1,000
__
470
0.8
0.4
166
38
87
3.9
92
186
<0.005
<0.01
0.16
0.004
<0.006
<0.004
0.07
0.38
<0.001
0.18
0.028
0.06
cO.COl
0.14
3.39
Average
21
<3
9
0.72
6.7
<0.2
<0.5
6.1
0.14
0.13

<2

0
8.2
804
1,118
..
406
0.7
0.35
139
46
81
3.9
80
206
<0.004
<0.01
<0.11
0.004
<0.007
<0.006
0.06
0.46
<0.001
0.22
0.04
0.05

-------
TABLE C-4.  WELL 3B GROUNDWATER QUALITY RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOO
TOC
CCE
Total N
NHa-N
N org
N03-N
Total P
P04-P
Total coll form,
count/100 ml
Fecal coll form,
count/100 ml
pH, units
JDS
Conductivity,
inhos/cm
SS
Alkalinity
B
F
Na
Ca
Hg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Hn
N1
Pb
Se
Zn
SAR

July
22
<6
—
--
1.3
<0.2
1.2
<0.05
—
--
<2

-------
TABLE C-5.  WELL 1C GROUNDWATER QUALITY  RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOO
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total coli form,
count/100 ml
Fecal coli form.
count/100 ml
pH, units
TDS
Conductivity,
gmhos/cm
SS
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
Ni
Pb
Se
Zn
SAR

July
11
<6.0
—
0.54
7.9
<0.2
7.9
<0.05
<0.03
<0.03

<2

<2
8.1
1,277
1,700
--
—
0.5
0.4
214
69
115
4.7
136
375
0.006
<0.01
<0.1
0.004
0.012
<0.004
0.009
0.030
<0.001
0.360
0.080
0.011
<0.001
0.110
3.66
1976
Sept
10
3.0
--
0.78
1.1
0.5
0.5
0.15
0.27
0.22

23

<2
7.7
1.376
1,890
—
—
0.2
0.3
207
26
109
5.1
144
400
<0.005
<0.01
<0.1
<0.001
0.009
<0.004
0.066
0.010
<0.001
0.18
0.080
0.010
<0.001
0.138
3.97

Dec
32
5.5
—
--
1.2
0.9
0.2
0.1
0.12
0.05

8

<2
7.9
1,266
1,800
..
485
0.6
0.2
212
77
112
5.9
110
398
0.032
<0.01
0.08
0.008
<0.006
<0.004
0.10
0.08
<0.001
0.14
0.02
0.055
O.001
0.70
3.61
Mar 1977
18
5.0
13
--
1.2
0.0
1.1
0.1
0.06
<0.02

<2

<2
8.4
1,334
1,500
„
553
0.4
0.4
193
50
135
5.1
143
295
0.008
<0.01
0.15
0.002
<0.006
0.008
<0.004
<0.04
<0.001
0.15
0.010
0.10
<0.001
0.14
3.22
Average
18
<4.9
13
0.66
2.9
<0.4
2.4
0.1
0.12
<0.08

<9

<2
8.0
1.313
1,723
__
519
0.4
D.3
207
56
118
5.2
133
367
<0.013
<0.01
<0.11
<0.004
<0.008
<0.005
0.045
<0.04
<0.001
0.21
0.048
0.044
<0.001
0.27
3.61
Standard
deviation
10
<1.3
__
0.17
3.4
<0.4
3.7
0.04
0.11
<0.09

<10

0
0.3
51
167
„
48
0.2
0.1
9
23
12
0.5
16
49
<0.013

<0.03
<0.003
<0.003
<0.002
0.046
<0.029

0.10
0.038
0.043

0.29
0.31
Coefficient
of variation
0.56
0.27
..
0.26
1.17
1.0
1.5
0.4
0.92
1.1

1.1

1.0
0.38
0.04
0.10

0.09
0.5
0.33
0.04
0.95
0.10
0.10
0.12
0.13
1.0

0.27
0.75
0.33
0.40
1.0
0.73

0.48
0.79
0.98

1.1
0.09
                     143

-------
TABLE C-6.   WELL ZC GROUNDWATER QUALITY RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total collform,
count/100 ml
Fecal conform,
count/100
pH, units
TDS
Conductivity,
ymhos/cm
SS
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Hn
N1
Pb
Se
Zn
SAR

July
140
<6
—
0.4
<0.2
0.3
0.08
0.06
0.06
540
<2
8.0
830
1,210
--
—
0.3
0.4
120
63
79
5.5
94
186
<0.004
<0.01
<0.1
0.003
<0.006
<0.004
0.032
0.30
<0.001
0.130
0.055
0.010
<0.001
0.137
2.38
1976
Sept
199
46
--
1.6
<0.1
1.6
O.OS
0.07
<0.02
4,600 3
1,100
7.6
790
1.200 1
—
—
0.8
0.3
129
240
75
5.1
50
180
<0.005
<0.01
<0.1
<0.001
0.008
<0.004
0.008
0.007
<0.001
0.068
0.060
0.036
<0.001
0.047
1.86

Dec
86
6
—
2.9
<0.1
1.5
1.4
0.14
0.02
,500
<2
8.0
718
.110
—
440
0.6
0.2
133
47
80
5.9
70
103
0.007
<0.01
0.09
0.014
<0.006
0.08
0.12
0.50
<0.001
0.084
0.015
0.07
<0.001
0.041
2.74
Mar 1977
81
5
20
—
2.1
0.7
1.0
0.4
0.06
<0.02
<2
<2
7.9
702
907
—
420
0.5
0.2
156
30
68
5.5
109
108
<0.005
<0.01
0.15
0.004
<0.006
<0.004
0.09
<0.04
<0.001
0.12
0.024
0.15
<0.001
0.16
3.60
Average
127
<16
20
—
1.8
<0.3
1.1
0.5
0.08
<0.03
2,161
276
7.9
760
1,107
—
430
0.6
0.3
135
95
76
5.5
81
144
<0.006
<0.01
<0.11
0.006
<0.007
<0.03
0.063
0.21
<0.001
0.101
0.039
0.067
<0.001
0.10
2.65
Standard
deviation
55
<20
--
1.1
<0.3
0.6
0.6
0.04
<0.02
2,238
549
0.2
60
141
.-
14
0.2
0.1
15
98
5
0.3
26
45
<0.002
--
<0.03
0.006
<0.001
<0.040
0.052
0.23
--
0.029
0.022
0.061
--
0.06
0.73
Coefficient
of variation
0.43
1.25
--
0.61
1.0
0.55
1.2
0.5
0.67
1.0
1.99
0.03
0.08
0.13
--
0.03
0.33
0.33
0.11
1.0
0.07
0.05
.0.32
0.31
0,33
--
0.27
1.0
0.14
1.33
0.83
1.1
--
0.29
0.56
0.91
—
0.6
0.28
                    144

-------
TABLE C-7.   WELL 4C GROUNDWATER QUALITY RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOO
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total col 1 form,
count/ 100 ml
Fecsl collform,
count/100 ml
pH, units
TDS
Conductivity,
umhos/cm
SS
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
Ag
AS
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
Ni
Pb
Se
Zn
SAR

July
10
2
—
--
1.7
<0.2
<0.2
1.7
0.05
0.05
<2

<2
8.0
855
1.190
—
--
0.5
0.5
101
61
87
3.5
88
194
<0.004
<0.01
<0.1
0.003
<0.006
<0.004
0.012
0.030
<0.001
<0.003
0.050
0.008
<0.001
0.050
1.94
1976
Sept
10
2
—
--
1.6
<0. 1
<0.1
1.6
0.06
0.03
<2

<2
7.7
857
1,230
..
—
0.8
0.5
110
27
83
3.5
82
193
<0.005
<0.01
<0.1
<0.001
<0.006
<0.004
0.04
<0.04
<0.001
0.007
0.055
0.008
<0.001
0.068
2.37

Dec
28
4
	
0.65
2.1
<0.1
0.5
1.6
0.07
0.01
<2

<2
7.7
740
1,170
__
411
0.7
0.4
106
65
95
4.7
50
180
0.004
<0.01
0.03
0.007
<0.006
0.09
0.042
<0.01
<0.001
0.003
0.045
0.012
<0.001
0.041
1.96
Mar 1977
15
<2

-------
TABLE C-8.   WELL 6C GROUNDWATER  QUALITY RESULTS
          mg/L  Unless Otherwise  Noted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total conform,
count/100 ml
Fecal coHform,
count/100 ml
pH, units
TDS
Conductivity,
pmhos/cm
ss
Alkalinity •
B
F
Na
Ca
Hg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
N1
Pb
Se
Zn
SAR
July 1976*
14
6
—
—
<0.2
<0.2
<0.2
<0.05
0.04
0.04
<2
<2
7.2
855
1,950
—
—
1.4
0.3
230
217
103
4.5
250
280
0.008
<0.01
<0.1
0.003
0.008
< 0.004
0.008
0.020
<0.001
< 0.003
0.070
< 0.005
< 0.001
0.002
3.22
              "Only date sampled.
                       146

-------
TABLE C-9.  WELL 7C GROUNDWATER QUALITY RESULTS
          mg/L Unless Otherwise Noted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total collform,
count/ 100 ml
Fecal conform,
count/100 ml
pH, units
TDS
Conductivity,
umhos/cm
ss
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
*9
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Hn
N1
Pb
Se
Zn
SAR

July
<10
<6
—
..
<0.2
<0.2
<0.2
<0.05
0.04
0.04

<2

<2
7.5
951
1,400 1
—
—
1.1
0.6
120
62
85
2.7
138
280
<0.004
<0.01
<0.1
0.002
<0.006
<0.004
0.006
0.125
<0.001
0.003
0.055
<0.005
<0.001
0.002
2.32
1976
Sept
<10
<6
—
..
5.6
<0.2
<0.2
5.6
0.01
<0.01

<2

<2
7.8
962
,590
..
..
0.8
0.6
147
230
91
2.7
156
200
<0.005
<0.01
<0.l
0.002
<0.006
<0.004
<0.004
<0.04
<0.001
<0.003
<0.005
<0.005
<0.001
0.003
2.08

Dec
—
--
—
_.
«
--
--
--
--
--

••

--
—
--
—
w_
..
—
—
<•*
«
—
—
--
-
—
«
--
«
..
..
«
•-
..
--
• <•
-•
—
--
••
Mar 1977
22
3
6
„
12.2
0.0
1.2
11.0
0.05
<0.02

23

<2
7.8
1.260
1,580
<••
556
1.0
0.4
219
54
108
4.7
284
194
<0.005
<0.01
0.20
0.06
<0.006
<0.004
<0.004
0.24
<0.001
0.015
0.022
0.02
<0.001
0.06
3.96
Standard Coefficient
Average deviation of variation
<14
<5
»»

<6.0
<0.2

-------
TABLE C-10.   WELL 8C GROUNDWATER QUALITY RESULTS
           mg/L Unless Otherwise Noted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total coli form,
count/100 ml
Fecal coli form,
count/100 ml
pH, units
TDS
Conductivity,
umhos/cm
SS
Alkalinity
B
F
Ma
Ca
Hg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
Hg
Mn
N1
Pb
Se
Zn
SAR
1976
July Sept
18
<6
..
„
<0.2
<0.2
<0.2
<0.1
0.1
0.1

-------
TABLE C-ll.  WELL 9C GROUNDWATER QUALITY RESULTS
           mg/L Unless Otherwise, No ted
Constituent
COD
BOD
TOC
CCE
Total N
NH3-N
N org
N03-N
Total P
P04-P
Total conform,
count/100 ml
Fecal collform,
count/100 ml
pH, units
TDS
Conductivity,
umnos/cm
SS
Alkalinity
B
F
Na
Ca
Mg
K
Cl
S04
Ag
As
Ba
Cd
Co
Cr
Cu
Fe
rig
Mn
N1
Pb
C*»
Se
Zn
SAR
1976
July Sept Dec
52
0.3
—
0.71
10.2
<0.1
0.3
9.9
0.13
0.0

33

8
8.3
1.274
1,940
••- -- . ..
579
1.1
0.25
230
-- -- loo
107
5.5
265
16
0.005
<0.01
<0.02
0.007
<0.006
<0.004
0.032
<0.01
<0.001
<0.003
-- — 0.035
<0.05
<0.001
0.076
-- 3.81
Mar 1977
<8
<2
5
—
9.5
0
0.4
9.1
<0.02
<0.02

540

540
7.5
1,312
1,510
— —
568
1.0
0.4
219
59
111
5.1
267
194
<0.005
<0.01
0.06
<0.001
<0.006
<0.004
<0.004
<0.04
<0.001
0.018
0.016
0.015
<0.001
0.004
3.88
Standard Coefficient
Average deviation of variation
30
1.2
—
0.71
9.9
<0.05
0.4
9.5
<0.08
<0.01

287

274
7.9
1.293
1,725

574
1.1
0.3
225
80
109
5.3
266
105
<0.005
<0.01
<0.04
<0.004
<0.006
<0.004
<0.018
<0.03
<0.001

<0.03
<0.005
mm
^^
<0.020
<0.03
„
<0.011
0.013
0.025
— —
0.051
0.05
1.0
1.0
— —
__
0.05
..
0.18
0.06
1.0
• *

1.3

14
0.08
0.02
0.18

0.01
0.06
0.33
0.04
0.36
0.03
0.06
0.004
0.83


0.75
1.3


1.1
1.0

1.0
0.5
0.76

1.3
0.01
                     149

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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO.
EPA-600/2-78-084
4. TITLE AND SUBTITLE
LONG TERM EFFECTS OF L/
DOMESTIC WASTEWATER H
Infiltration Site
2.
iND APPLICATIO
ollister , Californ
7. AUTHOR(S)
Charles E. Pound, Ronald W. Crites, James
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Metcalf & Eddy, Inc.
Palo Alto, California 94303
12. SPONSORING AGENCY NAME AND ADDRESS
Robert S. Kerr Environmental Research Lab-
Office of Research and Development
U. S. Environmental Protection Agency
Ada, Oklahoma 74820
3. RECIPIENT'S ACCESSION1 NO.
5. REPORT DATE
N OF Aoril 1978 issuine date
tia Ranid 6. PERFORMING ORGANIZATION CODE
8. PERFORMING ORGANIZATION REPORT NO.
V. Olson
10. PROGRAM ELEMENT NO.
1BC611
1 1 . CONTRACT/GRANT NO.
68-03-2361
13. TYPE OF REPORT AND PERIOD COVERED
.Ada. OK
14. SPONSORING AGENCY CODE
EPA-600/15
15. SUPPLEMENTARY NOTES
16. ABSTRACT
The objective of this study was to evaluate the long-term effects of applying
municipal wastewater after primary treatment to the land using the rapid infiltration
technique. This was accomplished by analyzing groundwater quality and soil
chemistry at a site with a long operating history.
Primary municipal effluent has been applied continuously to rapid infiltration
basins at Hollister, California, for more than 30 years. The current daily flow is
43.8 L/s (1.0 Mgal/d) . Annual wastewater application equals 15.4 m (51 ft) to 20
infiltration basins intermittently flooded for 1 to 2 days every 14 to 21 days, dependin
on basin size and season of year.
Infiltration rates were determined, subsurface hydrology was logged, and
water table response to wastewater application was monitored. A sampling and
analysis program covering a 1 year period included samples from (1) primary
effluent, (2) onsite and control site soil profiles, (3) groundwater at the site and
upgradient and downgradient of groundwater movement from the site.
17.
KEY WORDS AND DOCUMENT ANALYSIS
a. DESCRIPTORS
groundwater recharge
soil properties
trace elements
water chemistry
13. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
b.lDENTIFIERS/OPEN ENDED TERMS
rapid infiltration systems
jrimary pre-treatment
(wastewater)
and application
municipal wastes
wastewater treatment
19. SECURITY CLASS (This Report)
TTNCTfASPTFTF.n
20. SECURITY CLASS (This page)
TTMf T A SfJTpTp-n
c. COSATI Field/Group
43F
91A
21. NO. OF PAGES
166
22. PRICE
EPA Form 2220-1  (9-73)
                                                                                 150
» u.». «OVB«HBIT muraia omcii H7i—7 57.140/682 7

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