Ecological Research Series
NUTRIENT DIVERSION:  Resulting Lake Trophic
                State  and Phosphorus  Dynamics
                                  Environmental Research Laboratory
                                 Office of Research and Development
                                 U.S. Environmental Protection Agency
                                        Corvallis, Oregon 97330

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                 RESEARCH REPORTING SERIES

 Research reports of the Office of Research and Development, U.S. Environmental
 Protection Agency, have been grouped into five  series. These five  broad
 categories were established to facilitate further development and application of
 environmental technology. Elimination of traditional  grouping was consciously
 planned to foster technology transfer and  a maximum interface in related fields.
 The five series are:

     1.     Environmental Health Effects Research
     2.     Environmental Protection Technology
     3.     Ecological Research
     4.     Environmental Monitoring
     5.     Socioeconomic Environmental Studies

 This report has been assigned to the ECOLOGICAL RESEARCH series. This series
 describes research on the effects  of pollution on humans, plant and animal
 species, and materials,  Problems  are assessed for  their long- and short-term
 influences. Investigations include formation, transport, and pathway studies to
 determine the fate of pollutants and their effects. This work provides the technical
 basis for setting standards to minimize undesirable changes in living organisms
 in the aquatic, terrestrial, and atmospheric environments.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                         EPA-600/3-77-003
                                         January 1977
  NUTRIENT  DIVERSION:   RESULTING  LAKE  TROPHIC

         STATE AND  PHOSPHORUS  DYNAMICS
                      by
              Eugene B. Welch
          University of Washington
         Seattle, Washington   98195
          Research Grant R 800512
              Project Officer

             Kenneth  W. Malueg
Corvallis Environmental Research Laboratory
          Corvallis, Oregon   97330
CORVALLIS ENVIRONMENTAL RESEARCH LABORATORY
     OFFICE OF RESEARCH AND DEVELOPMENT
    U.S.  ENVIRONMENTAL PROTECTION AGENCY
          CORVALLIS, OREGON  97330

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                          DISCLAIMER
     This report has been reviewed by the Corvallis Environmental
Research Laboratory, U.S. Environmental Protection Agency, and approved
for publication.  Approval does not signify that the contents necessarily
reflect the views and policies of the U.S. Environmental Protection
Agency, nor does mention of trade names or commercial products constitute
endorsement or recommendation for use.
                                 ii

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                                FOREWORD

Effective regulatory and enforcement actions by the Environmental
Protection Agency would be virtually impossible without sound scientific
data on pollutants and their impact on environmental stability and human
health.  Responsibility for building this data base has been assigned to
EPA's Office of Research and Development and its 15 major field installa-
tions, one of which is the Corvallis Environmental  Research Laboratory
(CERL).

The primary mission of the Corvallis Laboratory is  research on the effects
of environmental pollutants on terrestrial, freshwater, and marine eco-
systems; the behavior, effects and control of pollutants in lake systems;
and the development of predictive models on the movement of pollutants in
the biosphere.

This report describes the effects of wastewater diversion to a mesotrophic
lake in Washington.
                                                A.F. Bartsch
                                                Director, CERL
                                     iii

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                         ABSTRACT


     Lake Sammamish, Washington, was studied during 1970-75 to determine
the response to wastewater diversion in 1968.   The results were compared
with a pre-diversion study by Seattle METRO in 1964-65.   Diversion re-
duced the phosphorus loading by about one third - from 1.02 to 0.67 g
P/m2.yr and about 119 to 68 yg/1 in the inflow.  Winter  total  P remained
constant at about 30 +_ 2 jjg/1 and no trend was shown in  chlorophyll a_ in
spring-summer with a year-to-year average of about 7 +_ 2 vg/1.  Water trans-
parency remained the same - a summer mean of 3.3 in Secchi disk depth.

     Paleolimnological  evidence suggests that the lake has probably been
near its present mesotrophic state for possibly 100 years with some
alteration during the late 19th and early 20th century logging activity
in its watershed.  A thorough survey in 1913 showed trophic state in-
dices at similar levels as today.  The lake has a history of relative
stability in trophic state even though P loading was decreased by about
one third and also increased by at least that much.

     The lake's biological state did not respond to the  P loading change
largely because the water column P content did not change.  Its stability
is in turn thought to be controlled by the anaerobic-aerobic  release
and sedimentation of iron and its complexes.  Plastic column experiments
In situ showed rates of anaerobic release to range from 3-5 mg P/m2-day
and measured sedimentation rates to exceed the release rates,  with the
greatest sedimentation occurring after turnover and the  reestablishment
of an aerobic water column, although as much as three-fourths  of the
water column P content could have come from sediments by the end of
summer stagnation it was largely unavailable for spring  phytoplankton
because of subsequent sedimentation with iron.

     The increase or excess in P loading during the greatest urbaniza-
tion impact in the 1960's may well have gone to the sediment as was
suggested by mass balance considerations if P sedimentation varies with
P loading.  Also the introduction of waste P into the major inflow
stream 2 km above the mouth, rather than directly into the lake, argues
for its sedimentation upon entering the lake.   Without diversion, how-
ever, loading would now be at 1.36 g P/m2.day and probably some increase
in water column P content in winter would be apparent.

     Seasonal dynamic changes in P and chl a_ during spring were studied
with a mathematical model that simulated those and two additional state
variables.  The model provided an interesting procedure  for studying
the mechanisms controlling the timing and maximum for the s-pring diatom
bloom.  The model should be extended to study the dynamics of P and Fe
in the fall-winter period.
                             IV

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                            CONTENTS

 Foreword                                                     iii
 Abstract                                                      iv
 List of Figures                                               vi
 List of Tables                                                ix
 Acknowledgments                                                x

 Sections

 1.  Conclusions                                                       1
 2.  Recommendations                                                   3
 3.  Introduction                                                      4
 4.  Geographic Description of Lake Sammamish                          7
 5.  Morphometric and Hydro!ogic Description of Lake Sammamish        15
 6.  Limnological Characterization                                    17
 7.  Methods and Materials                                            20
 8.  Results                                                          32
 9.  Discussion                                                       79
10.  References                                                       88

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                        LIST OF FIGURES

FIGURES                                                           PAGE
   1.     Land Use Map of the Lake Sammamish Watershed,               11

   2.     Vertical distribution of percent water,  percent
         organic matter, and phosphorus as a fraction of
         the dry weight and organic matter of the sediment.
         Open circles represent values from Lake  Washington
         cores (Shapiro ejtal_., 1971).                             12

   3.     Location of sampling stations and bathymetric             2_
         map for Lake Sammamish.
   4.     State variables, transfer processes, and functional
         equations in the phosphorus model for Lake Sammamish
         See Tables 8 and 9 for further explanation of process
         functions.

   5.     Mean concentrations in the photic zone (usually top
         8 m) of growing season chl a^ (Mar-Aug) and Winter
         (Dec-Feb) total phosphorus and nitrate nitrogen
         relative to pre-diversion 1965 levels.  The 1965
         levels were: chl a_ 6.5 yg/£ (actually a  mean of 1964
         and 1965 data), total P 31  ug/£  and N03-N 390 yg/£
         The % blue green algae of the total phytoplankton
         volume was compared against the pre-diversion mean         _ft
         for June-October in 1965 and July-Oct in 1964 (67.5%)     -30
   6.     Total and ortho phosphorus concentration (mean of          .,
         epilimnion) at Lake Sammamish central station in 1974.
   7.     Oxygen isopleths in Lake Sammamish for 1973.
   8.     Dissolved oxygen, total  phosphorus, and  total  iron
         concentrations in Lake Sammamish for the 1972 turnover     .
         period (from Rock, 1974).
   9.     Dissolved oxygen, total  phosphorus, and  total  iron
         concentrations in Lake Sammamish for the 1973 turnover     ..
         period (from Rock, 1974).
  10.     Water column phosphorus content for turnover period
         (After Krull, 1973).                                       ™
  11.     Temporal variation in chlorophyll a (integrated means for
         photic zone) in Lake Sammamish, 1970 through 1973          Aft
         (1970-71 data from Emery 1972).                            ™

  12.     Mean seasonal variation in Secchi disk readings for
         Lake Sammamish.  Dashed line represents  mean for the       Rn
         plotted data (1970-71 data from Emery (1972)).             ou


                                  vi

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                        LIST OF FIGURES - (Cont,)


FIGURES                                                           PAGE

  13.    Total  Zooplankton   in Lake Sammamish,  1965 vs 1972.         52

  14.    Percent Composition of Zooplankton   Categories,  1972.       54
  15.    Phosphorus  sedimentation rates  in Lake Sammamish
        determined  at Stations 612 (28  m depth,  26 m Trap)
        and 2A (30  m depth, 27 m Trap).                             57

  16.    Total  sedimentation rates in  Lake Sammamish at Station
        612 (28 m depth,  25 m Trap) and Station  2A (30 m depth,
        27 m Trap).                                                 58

  17.    Changes in  Total  Iron and Total  Phosphorus in an Opaque
        Column from 8 August to 16 December 1974  (The column        6-|
        was partially reoxygenated by nitrogen aeration).

  18.    Changes in  Total  Iron and Phosphorus for 'an Opaque
        Column from 27 July to 8 January 1975.  (KHLPOy,  -          fi?
        0.236  mg/1  was added on day 120).              ^
  19.    Changes in  Total  Iron and Phosphorus in  a  Transparent       ^
        Column from 17 July 1974 to 8 January  1975.
  20.    Changes in  Total  and Soluble  Phosphorus  in the Lake
        Hypolimnion,  Station 6.2, from  29 May  to  27 November
        1974.   (Day 140 illustrates the commencement of  the         64
        annual  fall  overturn).
  21.    Release of  Total  and Soluble  Iron in the Lake Hypo-
        limnion,  Station  612, from 29 May to 27 November 1975.      65
  22.    Simulated and Observed Temperature  Profiles  for  Lake        68
        Sammamish (1970).
  22-b.  Simulated and Observed Temperature  Profiles  for  Lake
        Sammamish (1971).                                           69
  22-c.  Simulated and Observed Temperature  Profiles  for  Lake        7Q
        Sammamish (1972).
  22-d.  Simulated and Observed Temperature  Profiles  for  Lake        71
        Sammamish (1973).
  23.    Calculated  daily mixing depth in  Lake  Sammamish  during      7o
        March-July,  1972-72 (from Tang,  1975).

  24.    Simulated and observed Chi a_  content in the  epilimnion
        of Lake Sammamish during the  springs of 1970-73.            73
                                 VII

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                        LIST OF FIGURES - (Cont.)
FIGURES

  25.    Simulated and observed ortho-P concentrations in
        the epilimnion of Lake Sammamish during the springs
        of 1970-73.

  26.    Simulated and observed chl  a_ content in the epi-
        limnion of Lake Sammamish during the spring of 1972.
        Note relatively better simulation of the spring
        increase in  chl  ^ with the  modified  growth  model.

  27.    Phosphorus loading graph comparing the  relative.
        position of  three manipulated lakes.  Relationship
        after Vollenweider (1974).
PAGE
 75
 78


 86
                               vi ii

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                         LIST OF TABLES


TABLE                                                          PAGE

  1.    Summary of Basin Geography.                               7

  2.    Historical Evens of Interest in the Lake Sammamish
        Bain (After Fish, 1967).                                  9

  3.    Diatom Ratios for Lake Sammamish Core 08.                14

  4.    Summary of Pertinent Hydrologic and Morphometric        -ic
        Characteristics for Lake Sammamish.

  5.    Hypolimnetic (depth greater  than 15 m) Oxygen Content
        and "Actual" Oxygen Deficit  Rate.                        18

  6.    Trace Metal  Survey on October 24,  1971 for Lake
        Sammamish.                                              19

  7.    Summary of Routinely Monitored Physical, Biological      2s
        and Chemical Parameters.

  8.    Mathematical Expressions  of  Involved Subprocesses
        in the Phosphorus Cycling Model.                        30

  9.    Notations  and Units Used  Along With Values for
        Constants  for the Phosphorus Cycling Model.              31

 10.    Phosphorus Loading (Kg/Yr) to Lake Sammamish  before
        and after  Wastewater Diversion.   P Estimates  Are
        Based on a Normal  Water Year.                            35

 11.    Nitrogen Loading to Lake  Sammamish in Kg/Yr;
        11  tributaries  plus Issaquah Creek.                      36

 12.    Summary of Present Limnological  Characteristics in
        the Photic Zone (except oxygen deficit).                 39

 13.    Annual  and Growing Season Means  of Phytoplankton
        Chlorophyll  a. (weighted means  for  the euphotic  zone)
        and Daily  Rate  of Primary Productivity in  1970-1974.     49

 14.     Comparison ofJUigust Net Hauls 1913 vs 1972
        (Number per  m ).                                         51
 15.     Zooplankton  Species in  Lake  Sammamish.                   53

 16.     A  Comparison of P  Release Rates  From in  situ  Column
        Experiments  and Observed Hypolimnetic Changes in
        Concentration During 1972-75.                            66
                                 ix

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                     ACKNOWLEDGMENTS

     This report is a compilation of the thinking, work, discussions
and writing of faculty and a large number of students in the Civil
Engineering Department, University of Washington.  Although there is
not room to list them as authors, while in fact many of them really are,
they should be given special mention here.  Special mention should go to
Dr. D. E. Spyridakis and Dr. B. W. Mar for their active participation in
the direction of the sediment and modelling effort, respectively.  The
students who were active in research in Lake Sammamish and who completed
theses contributing to this report are in chronological order:  Dr. R. M.
Emery, Mr. C. E. Moon, Mr. M. Morton, Mr. S. Lanish, Mr. J. Krull,
Mr. F. W. Monahan, Dr. C. A. Rock, Dr. 6. Pederson, Ms. S. Guttormsen,
Dr. C. H. Tang, Mr. J. C. McDonnell, Ms. C. M. Noah, Dr. P. Birch,
Mr. R. L. Barnes and Mr. J. Mock.  Special thanks is also due laboratory
technicians Ms. S. Hamel and Mr. R. Tarn.
     Partial support for this work was also provided by the IBP-Coniferous
Forest Biome, National Science Foundation Grant No. GB-20963.   This is con-
tribution no. 263 from the Coniferous Forest Biome.

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                               SECTION 1
                              CONCLUSIONS

1.  About 7,000 kg/yr of phosphorus was diverted from Lake Sammamish
    with waste water in 1968.  This amounted to an aerial loading reduc-
                                                     2
    tion of about one-third - from 1.02 to 0.67 g P/m -yr or a reduction
    in mean flow weighted inflow concentration from 119 to 68 ug/1.
2.  Continuous monitoring of the major inflow stream showed that daily
    observations were necessary to avoid missing 25-30% of the annual
    input of P.  High concentrations of P (200-300 ug/1) and high flows
    occurred together on four separate days in 1973-74 and four in  1974-
    75 which amounted to such a high fraction.
3.  The reduction in loading had no measurable impact on lake water con-
    centration of total P and chlorophyll a_t water transparency or
    hypolimnetic oxygen deficit rate.  The blue green fraction of the
    phytoplankton did seem to have declined significantly - by over 40%.
4.  Although most trophic state indicators did not change, the lake
    nevertheless remains mesotrophic with a mean summer transparency
    (Secchi disk depth) of 3.3 m and a maximum of near 6 m.  This has
    been the state of the lake since early in this century and possibly
    since the settlement in the watershed of European man although  log-
    ging had an impact in the late 19th and early 20th centuries.
5.  Maintenance of lake trophic stability is most probably due in large
    part to the constancy of the winter P concentration, which in turn
    is controlled by rather high anaerobic release rates from the sedi-
    ment (3-4 mg P/m -day) in summer, but more importantly high sedi-
    mentation rates following autumn turnover of usually about 4 mg  P.m •
                                      2
    day, but initially up to 14 mg P/m -day.   Much of the P fluctuation
    was correlated with iron, which is no doubt controlling the P cycle
    in the lake - anaerobic release and aerobic sedimentation.   Much of
    the higher pre-diversion P load evidently did not occur in the water
    column, was thus unavailable to algae, and only served to increase
    the sedimentation of P through the above and other processes.
                                   1

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Although the effect of a one-third reduction, as well as a similar
increase, in P load to Lake Sammamish was not apparent, the diver-
sion surely had a preventative value.  Without diversion loading
                       2
would now be 1.36 g P/m *yr and could well have exceeded the
stability mechanism(s) for water-column P.
A mathematical model with four state variables simulated P and
chl ^concentration reasonably well, but the model served primarily
to study seasonal dynamics of the system.  Although the work had
only begun it has much promise for better understanding of the
lake's cycling processes.

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                           SECTION 2
                        RECOMMENDATIONS

Work should continue on the seasonal  dynamics phase and associated
modeling activity.  With more continuously monitored key variables
the carefully measured rates of P interchange with the sediments
could be effectively utilized in the modeling process to determine
the causative processes involved in maintaining such a steady lake
water concentration of P in winter.
Effort should also include the nearshore area where changes in
attached algal biomass should first appear as a result of the
increasing urban development and its  associated greater storm water
input to the lake.

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                           SECTION   3
                          INTRODUCTION

     Since the turn of the century, cultural eutrophication of the
Nation's waters has become a problem of ever-increasing magnitude.  The
rapid, sprawling growth of our communities has perturbed surrounding
watersheds, augmenting the point and non-point sources of nutrient sup-
ply to many lakes.  These artificial sources have stimulated primary
production, often culminating in massive, foul-smelling plankton blooms
that cloud the water.  Typically, such events arouse adverse public
reaction, with a resultant out-cry for corrective action.
     The strategies and schemes for remedial action run the gamut from
nutrient diversion to sealing the lake bottom with plastic sheeting.
Perhaps one of the most frequently applied solutions has been to divert
incoming nutrients away from an affected lake.  The results can be very
spectacular, as in the Lake Washington case (Edmondson, 1970, 1972),
but also extremely expensive.  The cost of the Lake Washington sewage
diversion project exceeded $125 million (Gibbs, et al., 1972).
     Subsequent to the Lake Washington project, the Municipality of
Metropolitan Seattle (METRO) initiated a similar program for Lake
Sammamish, which seemed to show early signs of movement to a eutrophic
state (Isaac, et al., 1966).  The secondary effluent from the city of
Issaquah, Washington, and waste from a milk processing plant were
diverted in September of 1968 at a cost of $3 million.  Located only
3 kilometers east of Lake Washington, Lake Sammamish was expected to
display a rapid improvement like its sister lake.  To determine if the
lake would respond to nutrient diversion and the extent and rate of such a
response, monitoring of the lake has been carried out from 1969 to 1975.
The Pre-diversion data on the lake consists of a two-year study by
METRO (Isaac, et al., 1966) and a one-day survey by Kemmerer, et al.
 (1923) in  1913.

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      In addition to continuous monitoring of limnological characteris-
tics, special studies of secondary production (zooplankton and fish),
nutrient exchange rates between sediment and water, phytoplankton up-
take  of nutrients, feeding rates of zooplankton, profundal bottom
fauna, and dynamic modelling of the phosphorus and nitrogen cycles have
been  conducted, as well as a careful evaluation of the nutrient (partic-
ularly P) income (Birch, 1974; Emery, 1972; Hendrey, 1973; Horton,
1972; Monahan, 1974; Moon, 1973; Pederson, 1974; Sturtevant, 1974;
Rock, 1974; Welch, et al., 1973; and Welch, et al., 1975).
      Most of this effort has been for the purpose of estimating the
change in the nutrient income, and defining observed changes in trophic
state indicators and the processes that have permitted the lake to re-
main  mesotrophic in spite of alteration of the P loading through the
diversion project.  In fact, core analyses have shown that several in-
dicators of trophy have remained unchanged for over 100 years.
      The diversion has subsequently been shown to have amounted to one-
third of the lake's P loading.  Apparently the lake's internal sediment-
water interchange mechanism controlled by iron has resisted P loading
                                                2
changes over a range of at least 0.7 - 1.1 g P/m -yr. and allows the
available water column P content to remain remarkably stable.  This may
well  be the principal cause for the lake's lack of response to diver-
sion.  However, stability may result for other reasons and could not be
expected to persist over a much greater range in loading and when viewed
over  the range of trophic states and loading that exist in the world's
lakes, the range examined in Sammamish appears rather small.
      For this project, the specific goals were to determine changes in
water column concentrations of nutrients, phytoplankton chlorophyll a_
and species composition, and transparency following diversion of
nutrients and determine, through measurement of external loading, the
resultant fractional decrease in nutrient income.  Further, the sedi-
ments were to be analyzed for N, P and C content and exchange rates be"
tween sediment and overlaying water determined in order to evaluate the relative

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 importance of the sediments as an internal source of P.  Lastly, the
 understanding of the  lake's behavior  in response to changing nutrient
 input was to be extended by incorporating knowledge of the principal
 process rates into a  mathematical model.  Models of the simple input-
 output, steady state  type described by Vollenweider (1969) and modified
 by Lorenzen (1973), as well as the multiparametric type (Tang, 1975),
 have been used.  The  steady state model application has verified the
 potential stability of the lake P content in response to changing P
 input while the multiparametric model has elucidated a more dynamic
 accounting of the P (and N) supply during the spring phytoplankton
 outburst.
     A principal advantage in defining the response of Lake Sammamish
 to nutrient diversion that was cited earlier, is its proximity and com-
 parability to that of Lake Washington.  Although many characteristics
 such as geologic age, water quality type, meteorologic conditions and
 flushing rate are relatively similar, some are distinctly different.
 Lake Washington is essentially twice the depth as Sammamish, does not
 completely lose its hypolimnetic oxygen, while Sammamish does, and
 apparently is more subject to wind mixing.  If not for those differing
 factors, the response of the two lakes to two fractions of diverted P
 could be considered with more validity.   The diversions removed nearly
 three-fourths of the entering P from Washington, while only about one-
 third was removed from Sammamish.   Whether the principal  reason  for no
 response in Sammamish is the control on water column P by the sediments
or the smaller fraction of P diverted can be debated.   The input-output
model suggests that it is the former, while Vollenweider and Dillon
 (1974) indicate that >50% diversion of P should be necessary for im-
provement.   To be sure,the year-to-year variation in the P input to
Sammamish has been about equal  to the fraction diverted.   Probably a
combination of the two is involved as will be emphasized in the report.

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                           SECTION 4
              GEOGRAPHIC DESCRIPTION  OF LAKE  SAMMAMISH
      The  waning  of the Wisconsin  glaciation  (14,000  BP)  left  the  Puget
 Sound lowlands dominated  by  striated  hills,  rolling  uplands,  and  deeply
 cut  troughs.  Today one trough  is  occupied by  Lake Sarranamish, a second
 by Lake Washington with the  meandering  Sammamish  River connecting the
 two.   A mild, maritime climate  now prevails, annually producing 90
 centimeters of precipitation and  a mean monthly temperature of 11.5°C
 (52.7°F).  Direct  sunshine is present 45 percent  of  the  daylight  hours.
 The  lake  is monomictic with  stratification beginning in  May and building
 to a  maximum in  August.   The thermocline is completely eroded by  late
 November  and the lake  remains homothermal until the  following May.
 Table  1 provides a summary of the  pertinent geographic conditions.
Table 1.
SUMMARY OF BASIN GEOGRAPHY
      Parameter
                             Lake Sammamish
Location
   Altitude (meters above mean sea level)
   Longitude
   Latitude
                          p
Size of Drainage Basin (km )
Duration of ice cover
Evapotranspiration (cm)
Evaporation (cm)
Precipitation (cm)
Maximum monthly precipitation (cm)
Minimum monthly precipitation (cm)
                                   12
                                122°05'W
                                 47°36'N
                                  253
                                  none
                                   23.7
                                    5.1
                                   90
                                   39
                                    0

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     The predominant surface stratum of the drainage basin is a light,
gray till.  This till is a hard unsorted mixture about 46 meters thick,
consisting of clay, sand, silt, and gravel.  Although the till is rela-
tively impermeable, thin beds of sand and gravel commonly yield small
quantities of perched water.  Aquifers transect the basin, with several
artesian wells Surfacing within the basin (Liesch, et al., 1963).  Coal
seams are located in the southern half of the watershed, while high
quality sand and gravel, refractory grade clay, quarry basalt and cin-
nebar deposits are scattered throughout the basin (Livingston, 1971).
     A geologic cross-section cutting through Issaquah in an east-west
direction shows base rock consisting of marine sedimentary rocks on the
west side of the Lake Sammamish valley.  On the east side is volcanic
rock with overlying layers of clay, advanced stratified drift, till and
sedimentary deposits (Liesch, et al., 1963).
     Prior to the arrival of European settlers in 1862, the Lake Samma-
mish basin was covered in a climax formation of Western Red Cedar
(Thuja plicata),  Western Hemlock (Tsuga heterophylla), and Douglas Fir
(Pseudotsuga taxifolia) (Hansen, 1938).  Heavy logging around the turn
of the century left the basin in second growth forest.  Today 80% of
the watershed remains in second growth, primarily red alder (Alnus
oregona) with scattered maple (Acer, sp.) and willow (Salix sp.).
The significant historical events in the basin are listed in Table 2.
     The population of the basin has grown from three families in 1862
to the present 40,000, the majority of the growth coming in the last 10
years.   The only sizeable concentration is located in the town of
Issaquah, population 4,500.   The town is comprised of the small  busi-
nesses required to support a residential community.   The only industrial
development is a dairy processing plant and a state  salmon hatchery.
Within the watershed are several gravel operations and a county sanitary
landfill.  Large residential  developments have been  built throughout the
entire west side of the lake.  The east side is dotted with small farms,
but the major portion of the land remains in second-growth.  A narrow
                                    8

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Table 2   HISTORICAL EVENTS  OF INTEREST  IN  THE LAKE SAMMAMISH BASIN
          (AFTER FISH,  1967).
   Date                           Event
 1862          Three families settled along  lakeshore
 1864          Three settlers and four Indians  killed  in  uprising
 1868          Hops started and become principal  agricultural crop
 1870          Census listed 28 men;  24 were farmers
 1880-1920     Logging very important,  lake  often covered with logs
 1887          Large scale  coal  mining  (1913-14 boom years), dies out
               in  1920's
 1888          Railroad built around  eastern shore
 1889          Donnelly mill  closed (large sawdust pile on lakeshore),
               large mill located at  Monohan (pop. 200)
 1900          Hops no longer farmed
 1912          Sammamish Slough  dredged, shortened from 19 km to
               10.5 km
 1913          Issaquah Sewerage  Agency formed
 1925          Town of Monohan burned down
 1940          Secondary treatment (trickling filter) built for
               0.15 MGD capacity
 1960          Population of  Issaquah is 1,870
 1968          Treated effluent diverted from Issaquah Creek
 1970          Population of  Issaquah is 4,314

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 strip  of  land  along  the east shore of the  lake has been subdivided into
 residential  tracts.  The upper valley drained by  Issaquah and Tibbets
 Creeks is  primarily  forested with scattered farms and small clusters of
 houses (Figure  1).
     Lake  Sanmamish  State Park, located at the south end of the lake,
 draws  over 600,000 visitors each summer.   Two commercial and an un-
 developed  county park are also located on  the lakeshore.  In addition,
 a  large county  park  touches the northern end of the lake and extends
 along  the  Samnamish  River.  Both Pine and  Phantom Lakes have county
 Parks  located  on their shores.  Presently  the waters of Lake Sammamish
 support a  variety of water sports, including water skiing, pleasure
 boating, boat  racing, swimming and fishing, besides providing an
 aesthetically  pleasing background for picnics and related activities
 carried out  at  the several lakeside parks.
     The primary point sources of wastewater within the basin were the
 town of Issaquah, the milk processing plant, and the fish hatchery.
 Since  1968,  the effluent from the town's trickling filter plant (568
 3                             3
 m  /d)  and  the milk plant (284 m /d) have been diverted out of the
                                                      3
 basin.  Today only the milk plant cooling water (227 m /d from ground-
 water) and the  hatchery passthrough water, which originally comes from
 Issaquah Creek, are  discharged to Issaquah Creek, and to  Lake  Sammamish.
 Only the sparsely settled east side and upper valley sections of the
 watershed  remain on  septic tanks.  Hence the percentage of the basin
 population on  septic tanks is small.
 PALEOLIMNOLOGY
     Paleolimnological  evidence suggests that Lake Sammamish has maintain-
ed a trophic state within the mesotrophic range for at least the past 100
years  in spite of settlement in the watershed by European  man and an  in-
crease and decrease  in the phosphorus income of at least one-third.   The
only shift in the 150 years of paleolimnologic history shown in the organic
matter profile (Fig.  2)  from Lake Sammamish is from 1880 to  1910,  using a
sedimentation rate of 3.5 mm/yr determined from a  recent historical event. This
                                 10

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                                                                 N
                                                                 A
                             LAKE SAMMAMISH
                                      Beaver Lake
Phantom ~=
Lake  S^^
                                         North Fork
                                         Issaquah
                 Tibbetts
                 Creek
              N
   Drainage
   Boundary
                                       East Fork
                                       Issaquah
                                       Creek
  :::  INDUSTRIAL
     COMMERCIAL
     RESIDENTIAL
                                                 x  *r
 Figure 1.  Land Use Map of the Lake Sammamish Watershed.

                                 11

-------
 Percent  Water
v-
10-

*E

£
120-
A
lAl
U.
0
530-
0.
Uf
40-
50-
"N 	



60
o
o
(

o

0
\
•
o
o
o










Figure 2. Vertical distrib
  Percent Organic Mat.
0    10    15    20     °
                      o
rag P/gm dry wt,
        5
mg P/gm organic  wt.
                                            • •   o
                                            - •  o
                                                                    0   10   20   30   40
Vertical distribution of percent waiter, percent organic matter, and phosphorus as a fraction
of the dry weight and organic matter of the sediment.  Open circles represent values from Lake
Washington cores (Shapiro et al., 1971).

-------
 bulge corresponds to the period of intense logging in the Pacific
 Northwest and a shift in pollen from confers  to alders.   The  organic
 matter bulge probably resulted from sawdust and bark  deposits from
 mill  and log rafting activities.   If a change from aerobic to anaerobic
 conditions had occurred during this period it surely  would have  been
 evident in the organic matter content.
      Comparison with Lake Washington results  (Shapiro, et al., 1971)
 shows that both phosphorus and organic matter content were higher than
 levels in Lake Washington in early years,  lower than  Lake Washington
 in recent years, but remained relatively constant^ over the past  century
 (Fig. 2).  The sediments in Sammamish clearly show less  impact of man
 than  in Lake Washington and are characterized most strongly by the  con-
 stancy of their contents.
      In  a more detailed  analysis of  historical  sedimentation using  Pb210
 dating,  four distinctly  different  rates were  observed with  the highest
 rate  having  occurred  between  1932  and 1944  following  logging activity.
 Although  the concentration  of  P in the sediment actually decreased,  the
 loading  rate to  the  lake during that  period may have been four times the
 present  loading  if the  increased rate of sedimentation is considered.
 The increased  sedimentation rates  are thought to be a result of increased
 erosion  following deforestation, which was at a peak during 1910-1930
 (Birch,  1976).
      A one-day study  of  the  lake by  Kemmerer, et al.  (1923) in August,
 1913,  strongly suggests  that  the lake was anaerobic and mesotrophic
 then.  Phytoplankton  species,  Secchi disk depth, and except for the ab-
 sence  of  Diaptomus, zooplankton species were  all similar then to those
 found  in  the lake today.
     The  pennate/centrate ratio of diatoms  in  the lake's  sediments have
also remained  relatively stable over the past  100 years (Table 3).
According to Stockner (1971) these values are  within the  1.0 - 2.0 range
for mesotrophy.  For more details of the paleolimnological results see
Rock (1974).  Also, Wiederholm (1976) has found chironomid species that
 indicate mesotrophy throughout a 50 cm deep core and a 100 year history.
                                 13

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 Table 3.  DIATOM RATIOS  FOR LAKE SAMMAMISH CORE 08.
Date
1972
1955
1940
1925
1910
1895
1880
1865
1850
Pennate/Centrate
1.2
1.0
1.5
1.1
1.2
1.1
1.5
0.9
0.9
     Thus, it appears that the trophic state of Lake Sammamish has re-
mained relatively unaffected by activities in the watershed since the
settlement of European man.  The associated increases in P income during
this period have been at least on the order of 30 to 40 percent and no
doubt much greater considering all potential  point and non-point sources,
                                  14

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                           SECTION  5
       MORPHOMETRIC AND HYDROLOGIC  DESCRIPTION  OF  LAKE  SAMMAMISH

      Lake Sammamish occupied  a  13  km section of the Sammamish River
 Valley after the  Wisconsin glaciation when  the retreating Vashon glacier
 left  a terminal moraine blocking the valley.   Today the lake level is
 controlled by a wier at the head of  the Sammamish River.  The deepest
 section of the lake is located  less  than 3  km  from the south end.  If
 15 m  is considered as the  division between  deep and shallow, the surface
 area  ratio of deep to shallow water  is

                      12km2
                7.8 km2  (shallow)

while the ratio of epilimnion to hypolimnion volume is 0.99.
     The study of water  currents has been limited to the movement of
Issaquah Creek water in  the lake (Moon, 1972).  During the period of
winter mixing the creek water dispersal is primarily influenced by wind
direction and velocity.  The water was sufficiently dispersed at a dis-
tance of 500 m to make the tracer undetectable.  Similar studies made
during thermal stratification showed the creek water plunging into the
metalimnion and dispersing in a fan-like pattern.   Additional morpho-
metric and hydrologic data are summarized in Table 4.
     Waste-water effluent from Issaquah entered Lake Sammamish through
Issaquah Creek.  In winter, during high flow waste water P entered
along with high levels of suspended sediment.  In  summer the waste P
followed the Issaquah Creek inflow water which probably entered the
open water area at depth.
                                  15

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Table 4.  SUMMARY OF PERTINENT HYDROL06IC AND MORPHOMETRIC
          CHARACTERISTICS FOR LAKE SAMMAMISH.
    Parameter                           Lake Sammamish
                                  .
 Surface Area of Lake (km )                      19.8
                  2
 Drainage Area (km )                            253
 Lake Volume (km }*                              0.35
 Depth
    Mean (m)                                    17.7
    Maximum (m)                                  32.0
    Epilimnion (m)                               8.8
    Euphotic (m)                                 7.3
 Width
    Mean (km)                                    1.5
    Maximum (km)                                 2.4
 Length  of Lake  (km)                             13.0
 Length  of Shoreline  (km)                        34.0
 Water Retention Time (yrs)                       1.8
                  3
 Stream  Inflow (km /yr}                           0.198
                   o
 Stream  Outflow  (km /yr)                          0.203
                             o
 Groundwater Infiltration  (km /yr)                0.0
 Groundwater Exfiltration  (km3/yr)                0.01
 Duration  of Stratification  (mos.)                7
     *
      influenced by wier
                                 16

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                            SECTION 6
                    LIMNOLOGICAL CHARACTERIZATION

TEMPERATURE
     Lake Sammamish  is a monomictic lake that begins thermal stratifica-
tion in May.  Maximum water column stability occurs by late August and
destruction of the  thermocline is complete by late November.  The tem-
perature range is from a minimum 5.5°C to a maximum 25.5°C.
LIGHT
     The depth of visibility has been determined by means of the Secchi
disk.  The annual mean for the six years of data is 3.3 m.  The lowest
seasonal mean (3.0m ) occurs    in the winter due to turbidity from the
winter mixing and runoff.  The springtime mean is only slightly higher
(3.1 m), but these  low values are due to the diatom pulse.  The light
penetration increases during the summer (3.5 m) and reaches its highest
values in the autumn (3.6 m).  The maximum Secchi disc measurements
usually occur in September, the deepest recorded being 6.1 m.
     Light extinction was determined by a submarine photometer.  The
bottom of the euphotic zone was considered to be at a depth receiving
1% of the surface light intensity.  The mean depth of the euphotic zone
is 7.0 m, while the range is from 5.0 to 12.5 m.
ALKALINITY AND pH
     Lake Sammamish has a pH range of 6.3 to 9.6 due to biological
activity.  Correspondingly the alkalinity as CaCOg ranges from 26 mg/1
(0.52 meq/1) to 42 mg/1 (0.84 meq/1), while the mean is 33.3 mg/1
(0.67 meq/1).
DISSOLVED OXYGEN
     During the winter, the oxygen content essentially remains at an
air saturation level, approximately 12 mg 02/1, due to continual

                                 17

-------
circulation.  The development of thermal stratification in early May
results in a clinograde CL curve that approaches zero oxygen levels
(0.1 mg Op/1) in the bottom waters by late July or early August.  The
hypolimnetic oxygen deficit continues to increase until early October.
By this time the entire hypolimnion (below 15 meters) has less than
1 mg 02/1.  Oxygen levels start to increase with the coming of the
autumnal circulation.
     The depletion of oxygen in the hypolimnion is dramatically shown
in the calculation of hypolimnetic oxygen content in early October
(Table 5).  Also shown are "actual" deficit rates.  The "actual" rates
are based on oxygen saturation of 12 mg 02/1 at 7.5°C occurring on
April 15th and the oxygen present at the end of stagnation.

Table 5   HYPOLIMNETIC (DEPTH GREATER THAN  15 m)  OXYGEN CONTENT AND
          "ACTUAL" OXYGEN DEFICIT RATE.
Total Hypolimnetic Total Hypolimnetic Number of
v 02 present at 0? present at end days of
Tear start of stratifi- of stratification stratifi-
cation (metric tons) (metric tons) cation
1970
1971
1972
1973
1120
1120
1120
1120
21.3
12.5
45.7
21.3
178
192
176
185
02 Deficit
Rate
(mg Og/
o
cm- day)
0.051
0.047
0.050
0.049
                                   18

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MAJOR AND MINOR IONS

     The results of a single survey are shown in Table 6.   Neither S04"

nor Cl" have been measured, while the only trace metals measured have

been Mn++, Zn++ and Pb++


Table 6.  Trace Metal Survey on October 24, 1971 for Lake  Sammamish

Location
Lk. Sam. 612
Surface
8 m
16 m
25 m
Islaquah Ck.
Tibbetts Ck.

Ca
12.

8.
8.
12.
24.

i
80

40
95
40
70

V.
3.

3.
3.
3.
8.

•- Pi
ig
42

44
68
70
15

N
8.
8.
8.
8.
9.
14.

la
43
47
15
17
31
59

K
1.01
0.98
0.94
1.00
0.94
1.52

Fe
40
63
280
1020
450
110

ypi
Mn
19
40
600
1660
35
20

Zn
376
300
35
34
318
150

Pb
0.5
0.5
0.8
0.8
0.9
4.2
 Outflow
 Sammami sh
   River          6.05   3.00    8.16   1.13     25     9     7    0.6
                                  19

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                             SECTION 7
                        METHODS AND MATERIALS

LAKE MONITORING
     The lake water column has been monitored at a central  location from
1970 through 1975 by students and faculty in the Civil  Engineering
Department, University of Washington.  Prior to the 1968 wastewater
diversion the Municipality of Metropolitan Seattle (METRO)  monitored
the water column at the same location for 1.5 years from July 1964
through 1965.  Thus, the effect of diversion is evaluated primarily
from the changes in trophic state indicators determined at that central
station designated as 612 in Figure 3.
     Sampling frequency has varied from monthly to weekly throughout the
period, with frequency generally increasing in recent years.  Twice
monthly was generally considered necessary particularly during the grow-
ing season.  After examining the 1970-71 data, Swayne (1973) showed that
a frequency greater than 20 days between sample collections would result
in an artificial smoothing in the temporal dynamics of many of the vari-
ables.
     One station was considered representative.  Emery (1972)
showed that there was no statistically significant difference between
chemical and biological variables at station 612 compared with a nor-
therly located station indicated by METRO as 611 (Fig.  3).
     During 1970-73 the photic zone was sampled at four depths based on
the amount of light received.  The depths corresponded to 95, 60, 30 and
1  percent  of incident light.  Secchi disk depths were used to locate
depths for given light levels based on a nomograph  determined by Emery
(1972).  This was largely for the purpose of insuring that maximum
photosynthetic rates were measured regardless of incident light varia-
tion.  The maximum in Lake Sammamish was found to vary from 30-60% of
incident light.  Samples for chemical analysis were also collected in

                                 20

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         Sommamish River
                                   OLYMPIC PENINSULA
                                 LAKE SAMMAMISH STUDY AREA
Sediment
 Trap-1
                           Issaquah Creek
     Figure 3.
                                   Tibbetts Creek
Location of sampling stations and bathymetric map for
Lake  Sammamish.

                  21

-------
the hypolimnion, one half the distance between the 1% depth and the
bottom and 1 meter above the bottom - a depth of 28 m.  This station
was not located at the lake's maximum depth, which is 31 m, but rather
at 28 m (Fig. 3).  Procedures were slightly modified in 1974-75 when
specific depths were regularly sampled throughout the water column -
surface, 5 m, 10 m, 15 m, 20 and 22 m.  Also, in situ oxygen and tem-
perature determinations were made at more frequent depth intervals than
other chemical measurements.
     The variables determined, the methods used and respective sources
for the methods are shown in Table 7.  The only method not explained is
phytoplankton volume, which has varied with investigations; METRO used
an inverted scope sedimentation along with specific cell measurements,
Emery (1972) used an upright scope, centrifugation and specifically
measured each observed alga cell, while subsequent work has been with
an upright scope, but with a sedimentation method in 1973 and a milli-
pore filter method in 1974-75.  Both later sets were developed with the
use of average volumes for a given species.   Although direct comparisons
of volumes are not comparable the percent volumes occupied by various
groups, for example blue green algae, is  considered valid.
IRON AND PHOSPHORUS AT OVERTURN
     The relationship between iron and phosphorus was studied at over-
turns in 1972 and 1973.   Samples for total P  total  Fe and oxygen were
determined at weekly intervals and every  2 meters in the water column.
Total  Fe was determined by atomic absorption (APHA,  1971).
NUTRIENT INCOME ESTIMATION
     Phosphorus and nitrogen loading into Lake Sanunairrish has been esti-
mated by measuring the concentration of total P and total N (only N03 in
water year 71) in several minor tributary streams.   METRO monitored 9
in 1964-65 (Isaac, et_ al_., 1966) and 12 in 1970-71 (Moon, 1972).  The
major surface inflow—Issaquah Creek—was also monitored during those
periods and again in 1972.
                                  22

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Table 7.   SUMMARY OF ROUTINELY MONITORED PHYSICAL,  BIOLOGICAL  AND
           CHEMICAL PARAMETERS
     Parameter
       Method
        Reference
 Physical
   Temperature Profile

   Light  Penetration
   Euphotic  Zone
 YSI and Hydrolab
   Temperature Probe
 Seechi Disk
 Submarine Photometer
Biological
  Primary Production

  Phytoplankton Volume
  Chi a
 14C Light-Dark
   Bottles
 Variable Sample
 Extraction with  90%
   Acetone
 Strickland and Parsons
      (1968)
 Strickland and  Parsons
      (1968)
Chemical
  Dissolved Oxygen
    Profile
  Total, Ortho-
    phosphate P
  Nitrate, Nitrite N

  Silicate

  PH,  Alkalinity
  Total  N
Azide  Modification-
  Winkler

Molybdate Complex-
  ing  Reaction
Cadmium-Copper
  Column
Molybdate Complex-
  ing Reaction
pH Meter
UV Light Oxidation
American Public Health
Assoc.  (APHA) et al.,
      (1971)
Strickland and Parsons
      (1968)
Strickland and Parsons
      (1968)
Strickland and Parsons
      (1968)
APHA et al.,  (1971)
Strickland and Parsons
     (1968)
                                 23

-------
sampled the streams once and Moon twice  per month for nutrient content
and flow, except that Issaquah Creek was continuously gauged by the
U.S.G.S. at a point about 1,9 km from the lake.  Moon collected 37
samples from Issaquah Creek in 1970-71 and 30 in 1971-72 (WY 71 and 72).
Issaquah Creek was shown by Moon (1972) to carry about 70% of the inflow
of P and 57% of the N (N03).
     Because of the variability in nutrient budget estimates from person
to person and by different means of data handling (see Rock, 1974), con-
tinuous monitoring of the Issaquah Creek nutrient content was begun in
the 1973 water year.  An automatic sample collector was installed in
the U.S.G.S. gauging hut and samples were collected every eight hours
for nutrient analysis.  Over 1000 samples were provided for analysis
from Issaquah Creek in the 73 WY.  This insured that periods of short
term peak runoff, in which total P was known to vary greatly, could be
accurately determined.  Daily collections were also made in the 1974
water year for total P and total N content.
     In Moon's (1972) water budget for the lake, subsurface was assumed
to contribute relatively little water to the lake in comparison to sur-
face stream flow.  Thus, the groundwater contribution of nutrient was
also ignored in the budgets.   Atmospheric input with rain to the lake
surface was included, however, by monitoring the rainfall, albeit
rather infrequently, during the 71  WY.  The final significant source is
the Washington Department of Fisheries salmon hatchery, which was
briefly evaluated and included in the below mentioned special study.
     To help resolve the problem of how much nutrient was actually
diverted in 1968, a joint study was undertaken with METRO to reevaluate
the contribution of the  Darigold milk processing plant and the Issaquah
sewage treatment plant effluents to the pre-diversion loading to the
lake.
                               24

-------
SEDIMENTATION
     Sediment raining down from the epilimion was collected in traps
located near the bottom at three additional stations besides the control
612 station.  Sediment was collected by four 10 cm diameter plastic
funnels which channeled the material into 50 ml centrifuge tubes.  These
four units were secured to a 30 x 30 cm polyvinyl chloride platform.
Traps were suspended at various depths in the lake, with emphasis here on
results from the bottom traps, 2 m above the bottom at all four stations.
    The traps were located after collection intervals ranging from one
week to several months by means of buoys submerged 1-2 m below the sur-
face.  This technique successfully avoided interference by the boating
public.  To minimize the decomposition of material, 5 ml of chloroform
was added to each centrifuge tube as a preservative.  A disadvantage of
this technique is that congregating zooplankton are trapped and killed
and then must be removed before analysis by filtration (0.5 mm) and then
detritus particles > 0.5 mm must be removed from the net and added back
to the sediment samples.
     Sediment analysis consisted of centrifugation at 9000 rpm for seven
minutes, decanted, dried at 60° C for 24-36 hours and weighed.  This was
begun immediately upon returning from the field.  Prior to this, samples
were examined microscopically to evaluate the qualitative nature of the
material as to allochthonous or autochthonous origin.
     Subsamples of dried sediment (.20-100 mg) were analyzed for C using a
Leco semi-automatic carbon analyzer and for N using the semi-microkjeldahl
method (Bremner, 1960).  Total P was determined according to the ascorbic
acid-molybdenum blue method in Standard Methods (1971) on digested sediment.
Sediment (20-100 mg) was digested in 10 ml  Teflon crucibles by incubating
for 12 hours at 120° C with 5 ml  of 40% HF.   Then 5 ml of concentrated
HN03 was added and treated at 240° C for 1-2 hours.  Oxidation was com-
pleted by further addition of 5 ml HN03 and cautious addition of 5 ml of
70% HCL03.   Just prior to evaporation samples were cooled, removed with
1  ml concentrated HCL, filtered through #4 prerinsed Whatman paper and
diluted with 50 ml  demineralized, deonized water.
                                    25

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 SEDIMENT-WATER INTERCHANGE
      Phosphorus release  from the sediment  in  Lake  Sammamish was deter-
 mined in  situ  by using six plastic  columns  inserted  into  the  sediment in
 the  vicinity of sediment trap 1  (Fig.  3).   Two opaque  columns and one
 transparent  column were  placed at depths from 10-12  m  and two transparent
 and  one opaque column were placed at 6-8 m  depths.
      Each  column  was 3.7 m long  with a weighted  base plate attached 0.6 m
 above the  bottom.  Volumes varied from 42 to  62  £  depending upon diameters.
 Sampling ports (1.3 cm dia)  were located at 0.3, 1.9 and  2.8 m above the
 sediment.  Tygon  tubes 15 m long were extended from  each  port to a sur-
 face  float.  Each column was  equipped with  a  plexiglass piston that
 allowed for  the extraction of sample water  and prevented  the influx of
 oxygenated water  and avoided  the formation  of a  vacuum Inside,
      After samples were  extracted deoxygenated water was  forced back into
 the columns.   About 5 I  or 10% of the column  volume  was removed with
 each  set of  samples.
      Analytical procedures for P, Fe, DO, pH  and alkalinity were the same
 as previously  indicated.
      Experiments were conducted  at various  time  intervals over a three-
year  period.   Usually, columns were sampled every 10-14 days.   Effort was
 made  to maintain the column samples under anaerobic  conditions by collect-
 ing the water  in chambers  previously purged wtth N«.   However, when filters
 clogged and were changed   Q? was  introduced  thus biasing the soluble P
 results.
      The columns similated two conditions in the lake.   The opaque columns
 represented  the dark hypolimnetic water while the transparent ones repre-
 sented the processes in  the euphotic zone.
      The experimental  periods in  1973-74 were May to July and  July or
August to December or January.   For the early experiments the  duration
ranged from 63-76 days for three columns.   For the later experiments the
periods ranged from 121-171 days.  In some  instances  the columns were
purged with N2 to immediately exhaust the DO while in others DO was
allowed to exhaust naturally via respiration.
                                   Z6

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 DYNAMIC  MODEL
      The model  developed  for  Lake  Sammamish  is made  up of  two major sub-
 models,  a mixing model, which computes  the daily mixing depth and tem-
 perature profile,  and  a phsophorus model, which utilizes the physical,
 chemical  and biological parameters pertinent to the  lake.  The model
 determines the magnitude  of the pertinent state variables.

 Mixing model
      The mixing model  computes  the temperature profile by  first calculat-
 ing the  thermal energy input  to the surface waters considering insolation,
 conduction, evaporation and back radiation.  The temperature throughout
 the water column is then  determined by  summing the temperature value
 from  the previous  day  at  each depth with the effect  of the diffusivity
 of energy within the water column at that depth.
      The mixing depth  is  then computed  by determining the  point in the
 water column where the kinetic  energy provided by the wind shear on the
 lake's surface coupled with the convective mixing energy due to insta-
 bility caused by temperature  differential, is equal to the  potential
 energy of the thermal  gradient  integrated from the surface.
      The  last operation the mixing model performs is to average the
 temperature from the surface  to the mixing depth and adjust the temper-
 ature at  each depth within this zone to an average value.

 Phosphorus
      To  facilitate modeling, the lake was divided into three zones, the
 epilimnion, defined as the layer between the surface and the mixing
 depth, the hypolimnion, defined as the  layer between the mixing depth
 and one meter above the bottom, and the sediment-water interface zone,
 defined as the layer containing the bottom sediments and the water one
meter above the bottom.  From these definitions it is observed that on
days when  complete mixing occurs the hypolimnion does not exist.
     The  phosphorus model  has four state variables:  phytoplankton (XI);
ortho-phosphorus (X2); zooplankton (X3); and detritus (X4).  These state
                                 27

-------
variables are represented by the pools (ovals)  in Figure 4.   The equa-
tions for the changes in those state variables  over time are also shown
in Figure 4.
     Table 8 lists the subprocesses which make  up the functional equa-
tions for the state variables.  The notation used for system constants
and variables is listed in Table 9 along with their definitions, dimen-
sions and the values for the constants.  As may be readily observed from
Table 8, all of the subprocesses, with the exception of photosynthesis
(Photo), are either Michaelis-Menton type or logistic functions.
     The arrows in Figure 4 show the model processes in graphic form
including sources, sinks and the exogenous variables (driving functions)
which are mixing depth  (H), temperature profile (T[I]), incident solar
radiation (RO) and inflow {Q. ).  Outflow is assumed equal to inflow in
                            in
this model and is also  notated Q. .  The sediment acts as a source and/or
sink for the phosphorus depending upon physical conditions.
     The phosphorus model was developed as a differential equation model
using a fourth-order Runge-Kutta technique to approximate the time
dependent changes in the state variables on a daily  basis.  The model  is
presently implemented  in both its original form and  as a difference equ-
tion model  using a variable time step  to insure that no state variable
changes more than five  per cent  (5%)  in any one time period.
                                 28

-------
    ^jf = -UPTAKE + PREG - DILUT

                 - P/l/day)
                                                                          PHOTO - PRESP - GRAZ - PDEA - SINK - PFLUSH

                                                                                                    - ch-/l/day)
                                                                                                       PRESP
                                                                                      Figure 4.  State variables,  transfer
                                                                                      processes, and functional  equations
                                                                                      in the phosphorus model for Lake
                                                                                      Sammamish.  See Tables 8 and 9 for
                                                                                      further explanation of process
                                                                                      functions.
dX4
~dT
ZDEA-PREG+DEXC-SED-DFLUSH

      +PDEA+DSR

    - P/I/day)
                                                                                                       ZRESP
                                                                                            ZRESP - ZDEA  +ZRESP
                                                                                             C/l/day)

-------
Table 8    MATHEMATICAL EXPRESSIONS OF INVOLVED SUBPROCESSES IN THE
           PHOSPHORUS CYCLING MODEL.
 PHOTO = kl.e(T/10).(2R/RO-dz)e(1"2R/RO'dz).(X2/(k2+X2))Xl
 PRESP = k3-T-Xl
 GRAZ  = k4-X3(Xl/(k9+Xl))
 SINK  = k5-dXl/dz
 PDEA  = klO.Xl
PFLUSH = Q1n-Xl/V
UPTAKE = CO.COT(PHOTO)
 PREG  = k7-X4
 DILUT = (.25.k6-X4)Qin/V
 ZGROW = C01-C02-GRAZ
 ZRESP = ZRES-T-X3
 ZDEA  = k8-X3
 DZD   = CO-ZDEA
 DEXC  = (1-C02)-CO-C01-GRAZ
 SED   = kll-dX4/dz
DFLUSH = (.3-k6-X4)Qin/V
 DPZ   = CO•CO!•PDEA
 DSR   = kl2-Ase(j-l/V(zn)

          where:  A  H = surface area of the bottom or sides in contact
                   sea   with the layer
                  V(zn)= volume of the layer
                                 30

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 Table 9.   NOTATIONS AND UNITS USED ALONG WITH VALUES FOR CONSTANTS
            FOR THE PHOSPHORUS CYCLING MODEL.
Notation Definition
XI
X2
X3
X4
X5
RO
R
T
H
kl
k2
k3
k4
k5
k6
k7
k8
k9
klO
kll
k!2
IRES
CO
CO!
C02
Values
PHYTOPLANKTON BIOMASS
PHOSPHORUS CONCENTRATION
ZOOPLANKTON BIOMASS
DETRITUS MASS
TOTAL PHOSPHORUS
INCIDENT SOLAR RADIATION
AVAILABLE RADIATION
TEMPERATURE
MIXING DEPTH
PHYTOPLANKTON MAXIMUM UPTAKE RATE
PHOSPHORUS HALF SATURATION CONSTANT
PHYTOPLANKTON RESPIRATION RATE
ZOOPLANKTON MAXIMUM GRAZING RATE
PHYTOPLANKTON SINKING RATE
PHOSPHORUS CONCENTRATION OF INFLOW
DETRITUS REGENERATION RATE
ZOOPLANKTON DEATH RATE
chl a HALF SATURATION CONSTANT
PHYTOPLANKTON DEATH RATE
SETTLING VELOCITY OF DETRITUS
NET SEDIMENT RELEASE RATE (ANAEROBIC)
ZOOPLANKTON MAXIMUM RESPIRATION RATE
PHOSPHORUS TO CARBON RATIO
CARBON TO chl a RATIO
FRACTION OF ZOOPLANKTON DIGESTION
Dimensions
yg-chl a/1
yg-P/1
yg-c/i
yg-P/1
yg-P/1
cal/cm /sec
2
cal/cm /sec
°C
meter
day"1
yg-P/1
day"1 "C"1
yg-chl a/1/
yg-C/1/day
m-day"
yg-P/1
day"1
day"1
yg-Chl a/1
day"1
m-day
2
yg-P/m /day
day"1 °C"]
mg-P/mg-C
Value
-
-
_
X2+X4
-
_
0.22*
0.62*
0.0005
0.015*
0.2
0.035*
0.3
1.0*
0.05
0.1*
2300.0*
0.0015
0.02
mg-C/mg-chl a_ 50.0
mg-C/mg-C
determined either in situ or in laboratory experiments
0.5
for
Lake Sammamish.
                               31

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                             SECTION 8
                              RESULTS
EXTERNAL NUTRIENT LOADING
     Rock (1974) reviewed problems in calculating the pre-diversion
nutrient loading in Issaquah Creek, particularly dealing with the choice
of either calendar or water year data.  By weighting the data on a
monthly time basis the short lived high peaks in P content and flow that
would bias the annual input estimate were damped out.  Thus, while
several different approaches (by different individuals) to calculating
nutrient load had produced values ranging from 23,000 to 11,000 Kg P/yr,
Rock established that about 16,000  Kg P/yr was the most reasonable
estimate for WY 1965 and 12,000 Kg P for the calendar year.  Total N
input was 170,000 Kg N for WY 1965; 122,000 of that was inorganic N.
These were determined by multiplying mean monthly flows by mean monthly
concentrations.
     Post-diversion loading estimates in Issaquah Creek showed consider-
able year-to-year variation.  Moon estimated 8,500 Kg P and 100,700 Kg
N (inorganic) in Issaquah Creek for WY 1971.  Guttormsen (1974) esti-
mated 198,000 Kg inorganic N in 1972-73 and 258,000 total N.  Rock
calculated 15,000 Kg P from the 30 samples collected in WY 72 and
7,126 Kg P for WY 73 when a complete data base on P concentration was
available.  Much of the year-to-year variation was due to flow since the
mean annual  flow in WY 1972 was 5.58 m3/sec and 2.91 m3/sec in WY 1973.
The input from June 1974 to June 1975 was 7,128 Kg P, almost identical to
the 1973 WY value even though the mean flow was higher—3.98 M3/sec.
     Rock found that most monthly or twice monthly sampling procedures
would result in underestimates of the true Issaquah Creek load by 13 to
26%.  However, if the 10 year annual flow and the annual mean concentra-
tion of 65 yg/1 P (12-312 yg/1) was used there was almost perfect agree-
ment with the true load.  The difficulty with estimating annual nutrient
input involves the simultaneously large, but short lived increases in

                                  32

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 flow and concentration.  Missing such important points will lead to great-
 ly underestimated loads.  For example, 25% of the annual P load came into
 Sammamish in the 1973 WY during two days in December and two days in
 January.  The mean flow for those four days was 22.6 m /sec and the mean
 concentration was 230 yg/1 P.  In 1974-75 there were four similar days
 in January with a mean flow of 20.7 m /sec and a mean concentration of
 303 yg/1 P.   Those four days contributed 30% of the 1974-75 load.   Thus,
 it is clear that daily observations of concentration as well  as flow are
 necessary, particularly during periods of high flow, if serious under-
 estimates of loading are to be avoided.
      Thus, it was important to attempt to normalize flow during pre- and
 post-diversion years to obtain an accurate estimate of the  quantity of P
 diverted.   For flows below 2.8 m /sec.,  flow and P  concentration in 1973
 were  inversely correlated, but above that flow they were directly  corre-
 lated.   Both correlations  were statistically significant.   The  process
 operating  that seems to produce  such variation  is a seasonal one.   As  the
 flows  increase in the fall  and winter the  material  accumulated  in  the
 streams  and  on the land during the  summer  is  flushed out.   During  that
 period concentration  may increase dramatically with  flow.   Once  the mate-
 rial has ,been  flushed out  in  late winter and  spring, increased flow may
 carry very little particulate  matter with  it  and concentrations may de-
 crease in spite of high  flows.   For  example,  for flows over 2.8 m3/sec
 the November-December-January  mean was 88 yg/1 while the  February-June
 mean was 39  yg/1.  The annual  mean was 65 yg/1.
     To accurately estimate the  fraction of P diverted, years of equal
 flow must be compared.  The 1973 WY was a very low flow year compared to
 the 1965 WY, however, when all post-diversion data, were used the result-
 ing correlation showed a relatively good fit  (r = 0.41) and it seemed
 reasonable to let the equation describe an average post-diversion year.
The mean flows for all post-diversion data through the 1973 WY is 4.3 m3/
sec.,  essentially the same as the mean pre-diversion flow of 4.25 m3/sec.
                                    33

-------
      The estimated P loading calculated by the above post-diversion equa-
tion is 9000 Kg.  Thus, comparison of this value with the most reasonable
pre-diversion estimate of 16,000 Kg P leaves 7000 Kg P diverted by the
METRO project in 1968.  Assuming Issaquah Creek is now 70% (Moon, 1972)
of the surface  income to the lake this means that about 35% of the total
surface input P was diverted.  Including an estimate for atmospheric in-
put to the lake surface lowers the diverted percentage to 34.  If around
7200 Kg P is considered as the post-diversion Issaquah Creek input, be-
cause of the close agreement in 1973 and 1974, then the diversion would
be 8800 Kg P or 43%, which is probably high.
      Another approach was used to estimate the fraction diverted and
that was to back calculate the quantity of waste-water P that was prob-
ably entering before diversion.   As part of this, METRO conducted a mass
balance of the  inputs and outputs to and from the Creek within the town
of Issaquah during high flow and low flow.  In addition the
Issaquah treatment plant flow before diversion was used with literature
values of dairy waste and sewage P to estimate the wastewater P load
before diversion (see Rock, 1974,for details).  The P loads measured
in the Creek did not balance and thus failed to provide usable data
because of diurnal variations, but the indirect approach provided an esti-
mate of 4,400 Kg P diverted, which  would have been 33% of the Issaquah
load (normal flow year), but only 255t of the lake's total load.  Using
this approach would mean that Issaquah Creek carried a pre-diversion
load of 13,400  Kg P.
     Because of the many uncertainties in the latter approach it seemed
most reasonable to stick with the previously mentioned approach; that
of correcting the post-divers ion data to a normal flow year, which the
1965 WY apparently was,comparing that to the 16,000 Kg best estimate for
Issaquah Creek  before diversion.  Therefore, the most probable estimates
of pre- and post-diversion phosphorus loads are  shown in Table 10. The
remaining wastewater P input comes from the salmon hatchery in Issaquah,
which is a rather crude estimate based on the two mass balances done on
the various inputs to Issaquah Creek by METRO.
                                34

-------
 Table 10.  PHOSPHORUS LOADING (Kg/Yr)TO LAKE SAMMAMISH BEFORE AND AFTER
            WASTEWATER DIVERSION.  P ESTIMATES ARE BASED ON A NORMAL
            WATER YEAR.

                                Pre-diversion (%)     Post-diversion (%)
  Issaquah Creek
     sewage and dairy waste       7,000 (34)                    0 (0)
     land runoff                  8,500 (42)                8,500 (64)
     salmon hatchery                500 (3)                    500 (4)
  11 Minor Tributaries            3,900 (19)                3,900 (29)
  Atmosphere to Lake Surf.           400 (2)                    400 (3)
  Total  external  loading         20,300 (100)               13,300  (100)
  Loading in g/m2 yr              1.02                         0.67
      In terms of normal year-to-year variation it was noted that for
the 1972 WY, which was wet, the Issaquah Creek loading was 15,000 Kg P.
For the 1973 WY, which was dry, the loading was 7,110 Kg P, with 9000
Kg P  as the estimate for an average or normal post-diversion year.
The respective flow weighted mean concentrations for the wet 1972 and
dry 1973 WY's were 85 and 78 ug/1.  The surprising thing is that 1974-
75 flow was about normal, 3.98 m3/sec versus the 11-year mean of 4.25
m3/sec, and the flow-weighted mean concentration was 57 jjg/1.  Thus,
there may be more variation in flow from year to year than in concen-
tration.
      If these post-diversion flow-weighted mean concentrations for 1973-
75 are compared with the pre-diversion concentration (68 versus 119  g/1)
then that represents a 43% reduction in concentration in Issaquah Creek
or 34% for all  inflows if Issaquah Creek is 70% of the P input.  That
estimated reduction  in concentration is in agreement with the reduction
in loading.
                                   35

-------
     Nevertheless, the P load can vary annually due largely to flow on
an order similar to the fraction directed.  This again emphasizes the
necessity of comparing relatively similar hydrologic years to estimate
manipulated loading changes.
     The nitrogen balance was treated less intensively than phosphorus
because the lake is primarily P limited and the difference in analytical
methods for NCK and organic N make the comparison of before and after
diversion data rather questionable.  However, the various estimates of
the total N input for two post-diversion years compared to METRO'S
estimate of pre-diversion input (NOg corrected to results with the
Cd-Cu reduction method - see Emery, 1972) are shown in Table 11.  To ex-
trapolate to total lake input,  Issaquah  Creek loading was assumed  to
be 57% of the total surface water  input.  See Appendix B for  1974  N
loading to Issaquah Creek—it was  nearly the same as that observed in
1972-73, the basis for Table 11.

Table 11.   NITROGEN LOADING TO LAKE SAMMAMISH IN Kg/Yr;  11  TRIBUTARIES
            PLUS ISSAQUAH CREEK.
                     Pre-diversion
                       (1965 WY)
Post-diversion   Post-diversion
(3/72 - 2/73;    (6/74 - 6/75
Guttormsen, 1974
Organic + NH3-N
N02 + N03 - N
Total N
Loading in g/m -yr
69,000
174,000
243,000
12.3
60,000
198,000
258,000
13;0
135,600
179,470
314,730
15.9
     The  increase noted in Table 11 cannot be considered significant in
the face  of the analytical differences between pre- and post-diversion
period investigators.  However, the ratio of N:P loading is 12 before
                               36

-------
 and  20  after  diversion,  suggesting that P should be most limiting under
 most circumstances.  Nitrogen fixation has been shown to be rather
 insignificant to  the total N supply  (J. Staley, personal communication1)
 Atmospheric input has not been measured, but should not account for
 more than  a few percent  of surface water input.
 RESPONSE TO DIVERSION
      Common trophic state indicators  in Lake Sammanrish have remained
 relatively stable, or at least no significant trend is apparent, in the
 six  post-diversion years the lake has been monitored.  Figure 5 shows
 the  mean concentrations  of total P and N03~N measured at station 612
 during  December through  February (the non-growth months) when the lake
 is well  mixed.  These values are weighted means in the photic zone,
 which corresponds reasonably well with the epilimnetic depth in this
 lake, and  are compared with the pre-diversfon values assigned as 100%.
 P remained very stable*  well within 10% of the pre-diversion level.
 Although NOo  decreased*the correction of prediversion data for a
 methods  difference (a factor of 2) makes the significance of that
 decrease rather doubtful.  In any event levels are very near 100% in
 the  last two  years.
      Chi a^ concentration was determined from March through August and
 weighted for  time, similar to the analysis of stream nutrient data men-
 tioned earlier, so that months with more frequent data points would not
 be weighted disproportionately heavy.   Although chl  a_ has varied con-
 siderably  from year to year no trend of either increasing or decreasing
 algal biomass in the lake is apparent.  The cause for such variations
 in spite of stable pregrowth P levels is not related to year-to-year
 variation  in  inflow P,  but probably to internal  mechanisms of P  supply
 as will  be discussed in later sections.   In association with algal
biomass,Secchi disk depth measurements have also not changed.  The  mean
summer season  Secchi  measurement remains at 3.3 m,  in  fact the greatest
Secchi disk value recorded is 6.1  m - a  pre-diversion  value.
^J. Staley, Dept. of Microbiology, Univ.  of Washington,  Seattle,
                                37

-------
     150 -
0)
c
o
•r-
vt

0)
•o
(U

Ou
0>
U

d)
O.
                                           v
                                            \   Blue Green   /
                                              x     Algae     '
            Nutrient
            Diversion
       25
                                               \/
     O
1964-
1965
Figure 5.
                  1970
                     1971
1972
1973
1974
1975
                   Mean concentrations in the photic  zone (usually top 3 m) of
                   growing season chl a_ (Mar-Aug) and winter (Dec-Feb) total
                   phosphorus and nitrate nitrogen relative to pre-diversion
                   1965 levels.  The 1965 levels were: chl a_ 6.5 yg/2,  (actually
                   a mean of 1964 and 1 65 data), total P 31 ugA and  NOo-N
                   390 yg A. The % blue green algae  of the total phytoplankton
                   volume was compared against the pre-diversion mean  for June-
                   October in 1965 and July-Oct  in 1964 (67.5%).

                                       38

-------
                                                             2
      Oxygen deficit  rate  has  remained  right at 0.05 mg 02/cm -day since
 diversion, a  little  more  than the pre-diversion rate, but with year-to-
                                        2
 year variation no more  than a few yg/cm  -day.
      The one  variable that seems to have changed significantly is the
 fraction of the growing season  (in this case June through October)
 Phytoplankton that is composed of blue green algae.  This value, deter-
 mined from surface and/or 1 m depth samples, has been consistently below
 that of the 1964-65  pre-diversion period, and except for one year has
 been markedly less.  Including all years the average is 46% below the
 pre-diversion level.
      The lake can clearly be considered as mesotrophic with respect to
 algal  biomass and productivity.   However, nutrient content and  oxygen
 Deficit would suggest a lake at least on the border between mesotrophy
 and eutrophy (Table  12).  From the standpoint of Carlson's (1974)  tech-
 nique  it falls into the 40-50 numerical range based on total  P,  chl  a.
 and Secchi  disk depth.

 Table  12.   SUMMARY  OF PRESENT  LIMNOLOGICAL CHARACTERISTICS  IN THE PHOTIC
            ZONE  (EXCEPT  OCYGEN DEFICIT).
Parameter
Chl a
Primary Production
Total P
Ortho P (photic zone)
NO.+NO.-N
«J c.
Oxygen Deficit (15-30 m)
Annual Mean
3
4.0 mg/m
494 mg C/m2/d
24.4 yg P/l
7.9 yg P/l
180 yg N/l
Growing season* or
Winter Means**
6.0 mg/m
700 mg C/m2/d*
31 yg P/l**
12 yg P/l**
275 yg P/l**
0.049 mg 02/cm -day
 *Growing season is March to August
**Winter is December to February
1
 Carlson, R., McGill Univ., Montreal, Canada,  unpublished manuscript.
                                   39

-------
WATER COLUMN PHOSPHORUS
     The cycle of P in the epilimnion  of Lake Sammamish is  somewhat
variable from year to year, but generally concentrations of both total
and ortho P are maximized at water column turnover during November -
December (Fig. 6).  Levels usually less than the maximum persist during
winter (Jan. - Feb.).  Ortho P is depleted to a slightly greater extent
than total P in spring and summer due to the conversion of P into algal
biomass.  The concentrations of ortho P reached near undetectable levels
in the summer during some years, e.g. 1974.
     The post-diversion annual mean epilimnetic content of total P  is
about 24 yg/1 and ortho P 8 yg/1.  The winter  (Dec. -  Feb.) mean for
total P  is  31 yg/1 and 12 yg/1 for ortho P.  One can see from Fig.  6
that 1974 represents a rather  typical year with respect to mean values
of the  two  forms  of  P.  The annual mean  total  P content for the entire
water column, which  includes the anaerobic hypolimnion in  summer,  is
36 yg/1.
     The question of the  stable  30 yg/1  winter concentration  in Lake
 Sammamish in spite of changed  P  loading is  of extreme  interest  and has
 been a  major thrust of this research.   Intensive  monitoring  (weekly)  of the
 turnover period during October through December was undertaken  by Rock
 (1974)  to approach the problem.
      The lake is stratified from mid May to mid November.   Portions of
 the hypolimnion are anoxic from late July or early August until turnover
 (Fig.  7).
      The pattern of D.O., total P and total Fe are shown in Figures 8
 and 9 during 1972 and 1973.  Note the inverse relationship between D.O.
 and Fe and  D.O. and total P.  Although concentrations in the hypolimnion
 seem potentially capable of at least doubling the epilimnetic concentra-
 tion at overturn it does not  happen presumably because once the hypolim-
 nion becomes aerated and Fe is oxidized much  of the hypolimnetic  P is
 resedimented.  The  high  levels of Fe and P were not observed in 1973 as
                                   40

-------
     o?
     *»x
     CD



     \Sl
     o
     o.
     to
     o
     o.
         10  -
                            M
M
N
Figure 6.  Total and ortho phosphorus  concentration (mean of epilimnion) at Lake  Sammamish
           central station in  1974.

-------
ro
      0.
      UJ
         30
               I       f      M     A     M       J

              Figure 7.  Oxyqen isopleths  in  Lake Sammamish for 1973.
I  '  A    '   S   "

-------
 D
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            EPfLIMNION
         1


                    11
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      0-
Figure 8.
                                  .•.-.-.
                                  m
                                   :'•:

                                  I
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                                         1
                                         m
                                         1
                                                                            I
                                                                            :•
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                                                                            •;- •

                                                                            1
           9-30   10-8    10-22   11-2    11-11   11-18    11-25   12-2   12-9
             Dissolved oxygen,  total  phosphorus, and total  iron  concentrations in Lake
             Sammamish for the  1972  turnover period (from Rock,  1974)
                                                                                    •t.o
1
p
                                                                                           •X,
                                                                                           M
                                                                                    •1.0
                                                                                    -0.5

-------
 n

 100
£50
10
         0J
D
100
 50-
       10
     Ss-
        8-1
       EPILIMNION
            •'*'•:






              1
                    1

HPYOLIMNION
                                                   IvM
                                                                   1
                                                                    s

                                                                   ;;$
           9-28    10-17     10-26   11-2    11-9    11-17   11-25   12-5
                                                                            1.0
                                                                     0.5  |5

                                                                         •
                                                                    1.0
                                                                   •0
                                                                   -0.5 E
  Figure 9.   Dissolved oxygen, total phosphorus, and total  iron concentrations in Lake

             Sammamish for the 1973 turnover period (from Rock, 1974)

-------
 in 1972 because the 0,0. content began to increase earlier (compare
 early November values) than in 1972,  Thus, earlier aeration of the
 hypolimnion initiated earlier oxidation and sedimentation rate increase
 in 1973.
      This efficient sedimentation of hypolimnetic P as a result of
 aerating  a previously anaerobic water mass may be the stabilizing  force
 for the water column P concentration.  Thus, added P to the lake,  par-
 ticularly if added to the hypolimnion during the warm stratified period,
 could simply be removed through the sedimentation process and never be
 available for spring-summer algal  growth.   In fact, the inflow from
 Issaquah  Creek probably does not enter the epilimnion, but rather  dives
 to the lower part of the metalimnion as shown by a dye study (Moon,
 1972).
      There has been an apparent change in  the peak water column  P  con-
                                                            O
 tent after diversion,  that is,  the  total amount  of P in g/m .   Figure 10
 shows  how these peak values have substantially decreased in  the  post-
 diversion years 1972-73.   Krull  (1973) suggested that the  high  pre-diver-
 sion peaks were related to the  rate of water column breakup  at  turnover,
 but Rock  (1974)  showed that was  probably not the total  cause  for the
 differences  in Figure  9 because  turnover or  temperature decline rates
 for 1964,  1965 and  1973 were similar.   However,  he  did  hypothesize that
                                                           O
 the  difference in calculated P  retention before  (0.848  g/m  -yr) and
 after  (0.489  g/m2-yr)  diversion  was  the result of  sedimentation differ-
 ences, because all  other evidence points to  the  release  rate from sedi-
ment remaining constant.   He  argued  that the  excess  pre-diversion sedi-
mentation  could have resulted in a build-up of loose particulate matter
on the lake bottom  and  thereby was supplying  the amount of P necessary
to explain the  higher  pre-diversion  peaks  (Fig.10) through mixing.
PHYTOPLANKTON
     Phytoplankton  biomass  shows a peak in the spring composed primarily
of diatoms.  The dominating genera during winter and spring are Melosira
and Ste^hanodisous.  During the summer and fall,  Fragilaria, Synedra.

                                     45

-------
     6
     0
           o	o 1964

           D	o 1965


           •	• 1972

           •	«1973
 D




 I
                               \!\
I   \
                                 I
                                ii
                                ;;
                                         11
                                         II
                                              D
Figure 10.  Water column phosphorus  content for turnover

           period (after Krull, 1973}
                            46

-------
 MelosIra, Rhizosalenia and Asterionella are the major diatoms.  The
 blue-green algae are comprised predominantly of Aphanocapsa, Microcystis,
 Coelosphaerium. Anabaena and Gomphosphaerium.  In 1973-74 the appearance
 of Aphanizomenon has been less pronounced than earlier while the abund-
 ance of Lyngbya has increased.  Predominant chlorophyseans are Oocystis,
 Spjiaerocystis. Closteriopsis, Chlamydomonas and Staurastrum.  Also pre-
 dominant in the phytoplankton of the lake is the chrysomonad Mallomonas.
      The pattern of phytoplankton biomass is one of typically the
 largest concentrations occurring in the spring - the diatoms (Fig. 11).
 During some years biomass increase begins early (March)  while in other
 years the increase is  delayed until April.   However, in  all  years  the
 chl  a_ content has subsequently declined to  5 yg/1  or less.   Thus,
 although the composition  of blue green algae comprised from  15 to  69%
 of the  June  - October  biomass in post-diversion years  (1970-75)  the
 abundance  of blue greens  is  not great.
      The maximum  chl ai  content  shown in Fig. 11 was  28 yg/1  in 1971.
 That  equals  the pre-diversion maximum in 1965.  However,  the highest
 value was  in  1975  - 37  jig/1  in  mid  April.
      The growing  season mean  chl a  content  is 7 yg/1 and  that  value has
 remained rather stable  during the 6  year study.  However, growing season
 Productivity  Can  vary as much asr+  100% from year to year (Table 13).
 Curiously, the years with low mean productivity are also the years when
 tne diatom bloom has started in early March.  Productivity was not
measured by Metro by similar methods so comparison of normalized (for
                                   47

-------
-P.
00
   ro   30
     E
£ 25

 oi
_J
jJ 20

X
Q.
O  1^
CC  I0
O
-J

o  10



      5



      0
               J

         Figure 11.
                           M
M
0
N
D
                Temporal variation in chlorophyll a_ (integrated means  for photic zone) in Lake Sammamish,
                1970 through  1973 (1970-71  data from Emery 1972)

-------
  Table 13    ANNUAL AND GROWING  SEASON  MEANS  OF  PHYTOPLANKTON  CHLOROPHYLL
              a. (WEIGHTED MEANS FOR THE  EUPHOTIC  ZONE) AND  DAILY RATE OF
              PRIMARY PRODUCTIVITY  IN  1970-1974.

                              Lake  Sammamish
Year

• — • 	
1970
1971
1972
1973
1974
Average
Chlorophy
(mg/m3)
Yearly

5.7
6.6
4.3
4.0
6.0
5.3
11 a.
Growing
Season
7.7
10.9
4.8
4.7
6.8
7.0
Primary
(mg
Yearly

711
467
799
496
789
652
Productivity
C/nr-day)
Growing
Season
899
575
952
545
904
775
 'ightjvalues before and after diversion is not possible.
      Transparency of the water column has been even more stable and less
 altered after diversion than chl  a..   As Fig.  12 shows the mean summer
 value has  remained very constant  around 3.5 ± 0.2 m.   Interestingly
 enough an  August value taken in 1913 by Kemmerer, et  al.  (1923)  showed
 3.3 m.

 ZOOPLANKTON
      Vertical  net  hauls  were collected  at  station 612  (see Fig.  3)  at
 frequencies  varying  from twice  weekly to once per month with the least
 frequency at periods of  low  reproductive activity.  Relatively little
 2°oplankton work had been done  in Lake  Sammamish  prior to 1970.  The
 first recorded zooplankton haul in Lake Sammamish was made by Kemmerer,
et al. (1923) in 1913.  Table 14 shows the results of this vertical  net haul
 (18 m) from August 13, 1913, versus the results from a net haul (25 m)
     on August 14, 1972.
                                   49

-------
0
I
0
 I
                                    -- 1
h-  •
a.
S  4
       64  65  70  71  72 73
             WINTER
                                64  65  70  71   72 73
                                       SPRING
u
1
E 2
x
f 3
O.
LU
0 4
5

-
-










































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-
i it ii i i ii ii i
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-


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U 	 U 	 1
, ,

,
       64  65  70  71   72 73
             SUMMER.
                                64 65 70 71  72  73
                                       AUTUMN
     Figure 12. Mean seasonal variation in Secchi disk readings for Lake
              Sammamish.  Dashed line represents mean for the plotted
              data (1970-71 data from Emery, 1972)
                              50

-------
  Table 14.  COMPARISON OF AUGUST NET HAULS 1913 vs 1972 (NUMBER PER  m3).
1913

Bosmina
Piaphanasoma

Cyclops
l£jschura
PJjPtomus

Mastjgocerea
Nothpjka (=Kell
P^lyarthra

Cladocerans
393
905
Copepods
5780
9805
-
Rotifers
393
Icottia) 113
113
1972

Bosmina
Diaphanasoma

Cyclops
Epischura
Pi apterous






51
331

3268
151
6573

0
0
0
      The two samples  are quite  similar except for Diaptomus being absent
  in the sample collected in Lake Sammamish in 1913, and it is the most
  common zooplankter now in the lake.  The difference in numbers in the
  other species with only 1 sample as a comparison is not significant as
  Population peaks may  be shifted several weeks from year to year.
      The first major  zooplankton study of Lake Sammamish was a portion
  of the METRO study of June, 1964 to December, 1965 (Isaac, et al . ,  1966).
  pigure 13 is a graph of total zooplankton (number per m3) in 1965 versus
  19?2.  The total numbers are quite similar and any difference probably
  can be explained on year-to-year or sampling variation.   For example,
  the major peak in both years was due to a rotifer bloom,  which reached
  Teo.OOO/m3 in 1965 and only about 90,000/m3 in 1972.   This could  be
explained by postulating that the 1965 sample was taken at the height of
 the bloom while the 1972 sample was a week on either  side of the  peak.
 Species  recorded during the 64-65 study are the  same  as  those found in
 tne lake at the present time.
                                     51

-------
       to

       '
       ro
en
ro
        OC
        LJ
        Q.
        UJ
        CD
            80-
            70
        ^  60
            50
40-
        =5  30
           20
            10
             0
                     1972
       /
      /
     /

    '1965
                              M
                                 M
N
          Fiqure  13.  Total  zooplankton in Lake Sammamish, 1965 vs  1972

-------
      Table 15 is a list of the zooplankton species that have been col
 lected in Lake Sammamish during this study.  Figure 14 shows the per
 cent numerical occurrence of the three major groups of zooplankton
 throughout the year and the most abundant is the copepod Diaptomus
 ash 1 andi.

 Table 15.   ZOOPLANKTON SPECIES IN LAKE SAMMAMISH.
 Copepods
  *Diaptomus  ashlandi
  *Epischura  nevadensis
  *Cyclops  blcuspidatus
CJadocerans
 *Daphnia thorata
 *D. schgilderi
 *Bosmi na longirostris
 *Diaphanasoma leuchtenbergianum
  Leptodora kindtii
  Scapholeberis kingi
Rotifers
 *Kellicottia longispina
  j<.  bos ton iens is
 *Polyarthra sp.
  Kerate11 a cochlearis
  J<.  Quadrata
 *Conochilus  unicorm's
  Collotheca mutabilis
  C^.  pelagica
  Notholca  squamula
  Ploesoma  hudsoni
  Gastropus sp.
  Synchaeta sp.
 Trichocerca sp.
 Filinia sp.
v
indicates  most  common  species.
                                53

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en
                                                          CLADOCERANS
          Figure 14.  Percent composition  of zooolankton categories, 1972

-------
  SEDIMENTATION
       Sediment trap data are summarized  in Table  16 while  seasonal
  events  are illustrated in Figures  14 and 15.   The trend  for  increasing
  sedimentation with depth was due  to relatively coarse and  dense alloch-
  thonous  material  transported into  the hypolimnion which  settled rapidly
  and  tended to be  intercepted by deeper traps.   Also in late  autumn sedi-
  ment released P continuously coprecipitates with  Fe-hydroxy  floes as
  the  thermocline erodes  and  upper hypolimnetic  waters are reaerated.
  The  Fe floc-P material  was  collected  by  deeper traps although at over-
  turn  some  of  it was also  collected  by  shallow  traps.  The  littoral
  (Station 3) and sub-littoral  (Station  1) traps  collected only about 70%
 as much as traps suspended at the same depths  in  pelagic regions   (Sta-
 tions 2a and  612) mainly because they were somewhat sheltered from aloch-
 thonous inputs during times of high stream flow.
      With regard to seasonal events (Figures 15 and 16),  high sedimen-
 tation rates occur during spring,  late autumn and  winter.  Microscopic
 examination of freshly collected material indicated that  high sedimen-
 tation rates in the spring resulted from settling  out of  the  diatom
 bloom which occurred in March-April.  During  summer the settled material
 was dominated  by  blue-green  algae detritus.   In situ experiments (Birch,
 1976) indicated that this material  decomposes much more rapidly than dia-
 tom detritus,  a factor  believed  responsible for the relatively small
 collections in traps at  that time.
      In late summer an  unusual organism tentatively identified as a
 bacterium Metal!ogeniurn  Personatum appeared in  abundance  in the sample
 from  26 m and  in the autumn  in the 15 m samples  as well.   This organism
 has also been  found in hypolimnotic  water samples  from Lake Washington
 (Shapiro et aj_., 1971).  The  radiating  tubules of  this organism are
coated with brown Fe and Mn containing compounds (Libby,  1972) to which
P may be adsorbed.  The  significance of this mechanism in  Fe,  Mn and P
cycling in Lake Sammamish has not yet been clearly  identified.
     Immediately after thermal destratification  (12/4/74)  there was a

                               55

-------
spectacular increase in the rate of sedimentation due to the chemical
effect of reaerated bottom waters.   Most of the Fe which had been
released following reduction of surface sediments during the anaerobic
period was reoxidized and precipitated as a floe.  Sediment released P
is sorbed on to these Fe (III) hydroxy compounds and co-precipitated.
Since sediment released P, until overturn, was contained in lower hypo-
limnetic waters and below the photic zone its contribution to algal
nutrition in Lake Sammamish is considered minimal.
     During winter high sedimentation rates of total material and of P
were due to increased input of allochthonous material resulting from
high stream flows.  More material, particularly total sediment, was
deposited at Station 2A becuase it is closer to the major inlet, Issa-
quah Creek.  High sedimentation rates of P during winter means that a
significant proportion of the external P income, most of which is  in-
troduced during winter, is rapidly deposited and effectively removed
as a source for spring-summer algal nutrition.
     A  comparison of primary production and sedimentation into the 7 m
trap  (Station  612) during  the productive  season  (March-October)  indicat-
ed that only a very  small  proportion  (%15%) of  this  produced material
settled out of the  photic  zone  as  detritus.  This  indicates  that nutri-
ents are  efficiently recycled  in the  trophogenic  zone during the stra-
tified  period. The  growing season rates of sedimentation were 43,
4.8 and 0.67 mg/m  day for C, N and P  respectively.
                                56

-------
   14
   12
   1O
O)
O
H
<
z
LU
O
UJ
(A
                         OVERTURN
                               r
                               I
                               I
                               I

                                               STN 612

                                               Stn 2A
       MAMJ   JASONDJ  FMAMJJ   A
                     1974                       1975
                             TIME IN MONTHS


Figure 15.  Phosphorus sedimentation rates in Lake Sammamish determined
            at Stations 612 (23 m depth, 26 M Trap) and 2A (30 m  depth,
            27 m Trap).
                                    57

-------
o

-------
  SEDIMENT-WATER INTERCHANGE OF P
       During the course of the column experiments there was  a  doubling  in
  ambient  lake temperature from 8°  C in early spring  to  20° C in  summer  and
  back  down  to 10°  C  in  autumn.   The column  DO content decreased  rapidly;
  from  8-11  mg/£ to 2-3  mg/5, in  30  days, with a subsequent slower decrease
  to as low  as 0.1  mg/H  in some  columns,   DO depletion in transparent
  columns  was  much  slower  due to photosynthesis,
       In  all  cases the  loss of  DO  was  accompanied by increases in P and
  Fe content.  Alkalinity  and pH responded consistently to the onset of
  anaerobic  conditions.  The opaque column's  pH  decreased to 6.5 and alka-
  linity increased from  32  to 39 mg/£ as CaCOg.  Subsequently pH increased
  to 6.8-7.0.  There was an initial  increase  in pH in the transparent
 Columns due to photosynthesis, but then they attained levels similar to
 the opaque columns.
      Rates of release of  Fe and P were determined by considering only
 positive  slopes on the concentration time curves and only after DO had
 decreased to levels  of 2-3 mg/£.  Values were excluded  where problems of
 ^2 intrusion were known to have occurred.
     As representative results from column  experiments   Figures  17-19
 are  presented.   Figure 17 shows changes in  total  P,  total  Fe and DO in  a
 column initially purged with N2 to effect early DO depletion.  That pro-
 cedure showed clearly that the time of incubation was indeed short  before
 Fe and P  began  to  release.  After  day 20  total  and soluble Fe  increased
 at rates  of 25  and 12 mg/m2 day, respectively,  and total and soluble  P
 also increased  at  3.9 and 4.3 mg/m2 day,  respectively.
     Figure 18  shows  results of an opaque column  allowed to  lose its  DO
 naturally,  which appeared nearly as fast as  the previously described
 experiment  in which the column  was purged.   The rates in this experiment
Were 3.15 and 3.22 mg/m2  day for total and  soluble P, respectively.  Total
and soluble Fe  increased  at 10  and 5 mg/m2 day.  Note the only slight
 increase  in P following the addition of P at 3 times the lake loading rate
On day 120.
                                     59

-------
     Although the DO decreased in the transparent column  more  slowly  than
in the opaque columns, release rates were as  great as in  the opaque
columns once DO became low (fig.  19}.  It took 90 days to reach  a  concen-
tration of 0.5 tng/5, in two such transparent columns,  one  at 7  m  and  the
                                                                       2
other at 10 m depths.  Total  and soluble P released at 4,4 and 4.1 mg/m
day once DO was depleted to the low value and total and soluble  Fe at 36
           2
and 32 mg/m  day, respectively.
     For comparison the observed changes in P and Fe in the hypolimnion
are shown in Figures 20 and 21.  During 1974, P, Fe and DO were  determined
at depths of 23-25 and 27 m at Station 612 - the 28 m Station,  The  re-
sults in Fig. 20 and 21 were calculated for a 5 m water column at that
deep station.  As shown P increased gradually after day 13 (July 8}  but
not until day 102 (Sept. 10) did the increase appear marked.  The rates
                                               2
of release for the period were 5.2 and 4.9 mg/m  day for total P and
                                           2
soluble P, respectively, and 25 and  12 mg/m  day for total and soluble
                                   o
Fe.  The DO loss rate was 0.69 mg/m  day which was the same as in one
opaque column - the  other two opaque columns showed rates of 0.54 and
         2
0.58 mg/m  day.
      Interestingly enough the maximum P  value reached, 290 yg/8, (1799
    2
mg/m  ) by day 165  (Nov. 13);was a concentration  comparable to most maxima
in opaque columns during  summer,  The maximum  Fe  content occurred the  same
time  as that of  P  -  3.4 mg/a   (2.97  g/m2).
      There was rather close agreement  in the P  release rates  in eight  of
                                                                  n
 nine  experiments in  1974.  The values  ranged from 2.6 to 4.4  mg/m  day
 for  total  P  with a mean of 3.4.   The one high value  of 12.6 mg/m2 day
 was  not included because  it was  thought excessive mixing during sampling
 caused that high value.   There was  no difference in  release rates between
 transparent or opaque columns once  DO had been reduced to 2 mg/SL  or below.
      Table 16 shows a comparison of all values of release of  P  by various
 investigators in Lake Samrnamish.  The only values that seem excessively
 out of line are those of Monahan (1974) in the spring which were later
 shown to be accounted for by  excessive mixing induced during  sampling.

                                     60

-------
UJ
                                                                     €>
          0
20
40
60     80      100
  TIME IN  OflYS
120
140
160
          DflYS
          DflYS
VERSUS DO
VERSUS TP
          OflYS   VERSUS TFE
        10  VflLUES
        10  VflLUES
         10 VflLUES
 Figure 17.  Changes in Total  Iron and Total Phosphorus in an  Opague
 Column from 8 August to 16 December  1974  (The column was partially de-
 oxygenated by nitrogen aeration).  From McDonnel (1976).
                                 61

-------
3.5
20
40
60     80    100   120
     TIME IN DRYS
                                                140   160    180
   DfiYS

   DRYS
 VERSUS 00
 VERSUS TP
 ^  DflYS  VERSUS TFe
  Figure 18.  Changes in Total  Iron and Phosphorus for an  Opague
  Column from 27 July 1974 to 8 January 1975.  (KH-PO. - 0.236 mg/1 was
  added  on day 120).  From McDonnell  (1976).         ^

-------
                20
        40
60     80     100
  TIME IN DflYS
120
140
160
          DflYS
          DflYS
          OflYS
VERSUS DO
VERSUS TP
VERSUS TFe
 9 VflLUES
 9 VflLUES
  9 VflLUES
Figure 19.  Changes in Total  Iron and Phosphorus in a  Transparent
Column from 17 July 1974 to 8 January 1975.  From McDonnell  (1976),
                              63

-------
    LflKE SflMMflMISH
                **STflTION  612«*
                DEPTHS [2O to 2 7m]
     0     20
     29 MAY J
            40     60    80     100
                       TIME  IN DflYS
120   140
 160   180
27 NOV 7^
DflYS  VERSUS DO
DflYS  VERSUS TP
DRYS  VERSUS TOP
                          14 VflLUES
                           14 VflLUES
                            14 VflLUES
Figure 20.   Changes in Total  and Soluble Phosphorus in the  Lake Hypolimnion,
Station 612,  from 29 May to 27 November 1974.  (Day 140 illustrates the
commencement  of the annual fall  overturn).  From McDonnell  (1976).
                              64

-------
           LflKE SfWIRMISH   **STRTIQN 612**
          0        20
          2k Jul 74
     60
TIME IN DflYS
80
100       120
   12 Nov 74
DflYS VERSUS DO
DflYS VERSUS TFe
DflYS VERSUS DFe
8 VflLUES
8 VflLUES
8 VflLUES
Figure 21.   Release of Total and Soluble  Iron in the Lake Hypolimnion,
Station 612,  from 29 May to 27 November 1975.  From McDonnell  (1976).
                              65

-------
Table 16.  A COMPARISON OF P RELEASE RATES FROM in situ COLUMN EXPERIMENTS
AND OBSERVED HYPOLIMNETIC CHANGES IN CONCENTRATION" DURING 1972-1975.
                                            lotal  P  2	Soluble P
                                                 mg/m  day
In situ columns
McDonnell (1975)
hypolimnion Station 612
1974
2.6-4.4
5.3
1.9-4.3
4.9
      in situ columns                       4. 0-6.0 (fall)
      Monahan (1974)                       17.0-26.0 (spring)
      In sjtu columns                       2.8 (summer)     2.6
      Horton (1972)                         6.2
     The effect of adding P to some columns (see Fig.  18)  with respect to
the maximum concentrations attained without addition suggests a situation
of equilibrium and sediment buffering in the lake.   P  attained a maximum
of 270 yg/ji following P addition to one column, while  two  other columns
and the deep lake showed maxima between 260-280 yg/Ji.   Overall maxima
ranged between 100-300 yg/£.   Near 300 ug/£ may represent  an equilibrium
value for the anaerobic bottom water assuming adequate contact with bottom
sediments occurs.
                                66

-------
  MODELLING
       Temperature profile output from the model  is presented  in  Figures  22a
     through 22d for days when corresponding observed data  were available
  for the years  1970-1973.  These figures show that the  simulated data
  give  a close  fit to the observed during this period.
       Figure 23  gives the simulated mixing depth for March-July of  1971
  and 1972.  When this is  compared to the epilimnion chl ^ values given
  in  Figure  24 it may  be noted  that the  earlier peak for the spring  diatom
  pulse  related closely to the  consistently greater mixing depths the
  model  had  simulated.   This  output has  caused  us  to reevaluate the  hypoth-
  esis that  the years  of early  blooms  also  have more light available to
  mixing  diatoms  because of a shallower mixing depth.  The model suggests
  that mixing, which effects continued nutrient entrainment, may be more
  important  than  light availability.  Another question raised about the
  1971 spring diatom bloom concerned the source of  P04-P being utilized.
 The P04-P pool, supply thought to be available was not sufficient to
 create a bloom of the magnitude of that which actually occurred.   The
 model was able to simulate a bloom of similar magnitude only when  fed
 P04-P from an external source.  Diffusion from the lake bottom was
 eliminated as the major source because of the enormous  diffusion  coeffi-
 cient which the model indicated would be required to effect  the  transfer.
 Littoral release and  surface runoff are other possible  sources to the
 P04-P pool  to consider.  The source from surface runoff is quite possi-
 bly asolution  in  the 1971  case  since there was  prolonged rainfall and
 subsequent  runoff shortly before the spring bloom began.
      Another  example  of information generated  by the model is  the fact
 that a  sensitivity analysis  of the model  parameters showed that if  the
 light intensity  required  for maximum  phytoplankton  production was held
constant, as first  hypothesized  (Tang,  1975),  the  sensitivity of the
model to that parameter was much  greater than could be expected in the
lake.  Accordingly, it  was felt that the function  in that form was not
an accurate representation and was therefore changed to its present
                               67

-------
      6 8  10       6 8 10
            (°C)
            6 8  10       6 8 10 12
M
0)
0)

°
  20
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  30

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     March ..27
April 11      April  25       May 9
           10    is
   0
                10      15     20    25
  10'
w
  20
   25
   30
                                5
         10
                               15
         20
         25'
         30.
       June  12
                 July 14
                                                         observed

                                                     —  simulated
   ,  Figure  22.    Simulated  and Observed Temperature Profiles  for  Lake
                   Sammamish  (1970).
                                      68

-------
57-9    .579
                               5  7  9
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                April 10     April 24
May 21
            10    15   18
                                 10    15     20
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S 15
 &  20
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            'June 30
                                  July 20
                                                          observed


                                                       —  simulated
      Figure 22-b.  Simulated and Observed Temperature Profiles for Lake

                    Sammamish (1971).
                                    69

-------


0

5

10
to 15'
0)
5 20.
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       March 11      April 4      April 18
                       April 27
              May 25
          10    15    20
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  20
  25
  30
10
15
20
25
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       10    15     20
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                                                     20
25
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       June 2
         June 13
           July 10
                                                            observed


                                                       	  simulated





      Figure  22-c. Simulated and  Observed  Temperature Profiles for Lake

                   Sammamlsh.  (1972)
                                       70

-------
      689
    0 ( ' f
  10         10
 (0
 M
 (X.
 0)
p
          0
            6 8 9  11 13
                                   5    10    15
5 15         15
  20         20
  25         25
  30         30
     March 31      April 27
                                10
                                15
                                20J
                                25
                                30
                                                        5   10     15  18
                                                 0



                                                 5




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                                                201




                                                25'




                                                30-•
                                  May  11
                                                            May 28
    0
5 .  10    15     20   5    10   . 15.    20  22     5-    10     15    20
• " " - i " '  i i  > ' '."  ' '   n . < t  ' - t - i ••'•-•• i I, •      I	« r . i i r r
   10
   15
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-------
    30
a.
    20
CL.
UU
O

  »,»  •
        • •  » «%
                                               •   •
         MARCH   APRIL    MAY    JUNE      JULY


       Figure  23.  Calculated daily mixing  depth in Lake Sammamish  during
                   March-July, 1971-72  (from Tang,  1975).
                                       72

-------
 20
 10
30
20
10
^    0
J   15


«"   10
 0
15
10
                                                      1970
                                                      1971
                                                      1972
                                                      1973
   MARCH    APRIL
                                  MAY
JUNE
  Figure 24.  simulated and observed chl a.
             content  in  the epilimnion of
             Lake Sammamish during the
             springs  of  1970-73.
JULY

	  simulated
	  observed
                               73

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form in which maximum production occurs at fifty per cent (50%)  of the
incident light level  (see PHOTO, Table 8 ).
     Figure 25 shows  a comparison of simulated to observed concentra-
tions in the PO.-P pool in the epilimnion.  It can be seen that,
although the model does not simulate the PO^-P pool exactly from year to
year, some of the simulations are reasonably accurate and well  within
experimental error.  It should be noted that no attempt was made to
calibrate the model to provide the best fit possible.

MODEL OF THE NITROGEN  CYCLE
     The above-described multiparametric  lake model considers both light
and  phosphorus as  potentially growth-limiting factors to Lake Sammamish
phytoplankton.  There  is some suspicion,  however,  that nitrogen may well
have some effect  on growth rates, particularly  in  the event of  increased
loading of  P.  In order to determine when nitrogen might be the limiting
nutrient and  to what extent  its  role would  be in controlling productivi-
ty,  and biomass,  a nitrogen  model has  been  developed which approximates
the  concentrations of  the  two major  inorganic forms, ammonia and
 nitrate(Noah,  1976).   These  forms of  N are  not  only  the most abundant
forms  of N, but  also  the most significant in  terms of plankton  algal
growth  rate.
     The following major  processes  can be evaluated  by  the model  on  a
daily  basis:   (1) assimilation  of inorganic N by phytoplankton,  (2)
 denitrification  of nitrate,  (3)  fixation  of ammonia  from molecular
 nitrogen,  (4) lake inflow and outflow concentrations,  (5)  mineraliza-
 tion of ammonia  from the  detritus pool, (6) nitrification of ammonia to
 nitrate,  (7)  reduction of nitrate to ammonia,  (8)  input of N in rain,
 and (9) sediment release  of ammonia.
      In order to evaluate some  of these rate  processes, it was  necessary
 to determine dissolved oxygen concentrations  as a controlling  factor.
 Rather than entering a complex  array of concentrations  over time and
 depth  as exogenous input, an oxygen model was developed.  The  reactions
                                    74

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   10
    0
   10
o?

 3.
£  0
a. 20
o
a.
o

I
o
10
    0
   10
    0
                                                      1970

                                                         *
                                                         1971
                                                         1972
                                                         1973
       MARCH    APRIL    MAY     JUNE     JULY
                                                      	 simulated
                                                      	  observed
       Figure  25. Simulated and observed ortho-P concentrations in the
                 epilimnion of Lake Sammamish during the springs of 1970-73,
                                       75

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on which the system depends are (1) production of oxygen via photosyn-
thesis, (2) consumption according to a biochemical oxygen demand func-
tion, (3) reaeration on the lake surface, and (4) benthal oxygen demand
at the sediment layer.
     Coordination and information transfer during the development of
the nitrogen model and the sensitivity analysis of the phosphorus model
revealed an interesting problem.  Both models used a Michael is-Menton
function to limit phytoplankton growth.  It was discovered that the
Michael is-Menton model has an apparent anomaly at limiting concentra-
tions when used in a difference equation model.
     As described by Mar  (1976)' the anomaly occurs at low concentrations
of nutrient and large biomass when the equation used is
                          C
             y = us ^ K   + S ^                (equation 1)
                       q
     where v = growth rate = Productivity/unit time = AB
               3                   biomass             B
           S = concentration of nutrient
          PS = growth rate of  infinite concentration
           K  = 1/2 saturation  constant

If S »  K  then y approaches yg and when S « K   y approaches zero.   It
is at  these latter concentrations  that the problem occurs.   If  S«K
then equation  1 reduces to
      or       AB  =  u   {j
 This  equation  says  that  the  change  in  biomass  (AB)  for a  given  concen-
 tration of substrate  is  a function  of  how much biomass in present.
 This  means that no  matter how little  substrate is available the amount  of
  Manuscript in review, ASCE.
                                  76

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  growth it can support can be increased by increasing  the  amount  of
  biomass assimilating it.   This is clearly incorrect and in  a  difference
  equation model  causes an  overestimation of growth  if  the  nutrient con-
  centration approaches zero.
       A new form of the equation  has  been proposed  by  Mar  (1976)  which
  is  of the form
 As  S approaches  zero  this  equation  reduces  to

                 AB  , „   / S
                 T  + ys  (T
                       S
                      ar  > B •
 This means that growth is dependent only upon the concentration of sub-
 strate at these values while at values where S » oB.  The equation
 reduces to the same form as the Michaelis-Menton function.
      This new equation has been incorporated in  the nitrogen  and phos-
 phorus models with the result output of chl  a_ for 1972 as  shown in
 Fig. 26.  The maximum growth rate was also reduced by 75% to delay the
 ch/s  increase.
      Comparison  of this  information  to that  provided by the earlier
 phosphorus model  (Fig. 24) shows a  much closer fit of the simulated
 data  to the  observed data.   Because  of its more accurate simulation
 the new equation  is  considered  a better approach  to  practically deal
 with modelling of  nitrogen and  phosphorus dynamics in  lakes.
     The model has not shown nitrogen  to  limit phytoplankton growth
 during  the March-to-July  period and  under the existing  P loading regime.
 Nitrogen may  indeed  prove to be temporarily limiting growth rate, or at
 least have an interactive role, in late summer or early fall  and/or under
 higher P loading rates.  Such extended application of the N model was
beyond the scope of this project, however.  The formulation,  parameter
evaluation and ^validation of this model was described by Noah  (1976).

                                  77

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    15

e*

"g  10

C
                                                  Observed
                                                  Simulated
                                                  Simulated with
                                                  modified growth model
 MARCH
                        APRIL
MAY
JUNE
                                                    JULY
Fig. 26.  Simulated and observed chl  a_ content in  the  epilimnion
         of Lake Sammamish  during the spring of 1972.   Note
         relatively better  simulation of the spring increase
         in chl  a_with the  modified  growth  model.
                              78

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                              SECTION  9
                             DISCUSSION

 HYPOTHESIS  FOR STABILITY
      Lake Sammamish  has shown  no  detectable changes in most of the per-
 tinent  lake quality  variables  in  the six years since one third of the
 phosphorus  income was diverted.   Water clarity has remained unchanged
 since diversion - the mean  summer Secchi disk depth is 3.3 m.  Mean
 winter  total phosphorus concentration has remained at about 30 yg/1 +
 2 yg/1  before and after diversion.   Chlorophyll a_, averaged over the
 growing season and throughout the photic zone, has remained at about
 7 yg/1 +_ 2 yg/1.  The only variable  that may have changed is the frac-
 tion of blue green algae, which shows an average decrease of 46 percent
 compared to pre-diversion (1964-65)  values.
      The apparent stability in the trophic state of Lake Sammamish, in
 spite of the watershed settlement by European  man beginning over 100
 years ago and the diversion of about one-third of its  phosphorus  income
 in 1968, can be largely attributed to the sediment-water interchange
 mechanism for phosphorus.   As much as three-fourths of  the  phosphorus
 increase into the water column during the stratified period in  May-
 November could  potentially be released as  soluble  P from  the anaerobic
 sediments  as shown  in plastic column  experiments.  The moderately high
 and  constant phosphorus  content in the sediment profile along with  the
 indication that the  lake's  hypolimnion has been anoxic in late  summer
 for  a  long time  suggests that the  P release rate has always been large
 in Lake  Sammamish.  Water column balances of P for the turnover period
 show a greater maximum before diversion (5-9 g/m ) than after diver-
 sion  (<2 g/m2).  Since there  has been no change in the winter water
 column content of P (31 yg/1) since diversion, the excess at turnover
 in pre-diversion years 1964 and 1965 was most probably lost to sedi-
mentation (Rock, 1974).
                                  79

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     The release and sedimentation mechanism is  thought to be largely
controlled by iron.   Phosphate is released in proportion to iron as
oxygen content reaches low levels and is eventually exhausted in late
summer.  Just prior to lake turnover the water column content of dis-
solved P is at the annual  maximum, most of which is in the hypolimnion.
Instead of these high concentrations being maintained throughout the
winter, much of that P is resedimented when turnover restores the aerob-
ic conditions and P decreases in proportion to Fe.  Presumably much of
this P is complexed with iron.  The relatively constant winter concentra-
tion of 30 yg/1 following the fall turnover appears to be residual, the
excess having been largely removed by the complexation with iron.  Pre-
sumably the additional phosphorus income before diversion had not in-
creased the pre-growth period residual P concentration because of the
ample  quantity of iron available for P complexation and sedimentation -
up to  0.9 mg/1 Fe at maximum anaerobiosis.  The large prediversion
quantities of P that appeared at fall turnover then could have come from
accumulations of flocculent sedimented P, that is, the quantity of
annual P  income removed by Fe over and above the  residual P level.  The
anaerobic nature of the hypolimnion  in this  lake  then seems to behave
as a greater effective sink for  P than if the hypolimm'on were aerobic.
That is,  the large quantities of Fe  released under anaerobic conditions
would  not have  been so released  in the aerobic state  (Fe  in the Lake
Sammamish hypolimnion is  10 times that  in Lake Washington) and  these
would  not be available for the precipitation of biologically mineralized
P.
     While  complexation of hypolimnetic  P with  iron  and  sedimentation
subsequent  to  turnover  (Nov.  - Dec.) is  probably  the principal  mechanism
controlling the residual  level of P, another contributing mechanism
operates  during Jan  - Feb.  To account  for  the  continual  decrease in
water  column P content  during those  later winter  months,  the  relatively
large  amount of P input  during  that  period  (runoff is usually high) is
believed  to be rapidly  sedimented along with the  large quantity of P
reactive  colloidal  material  brought in  through  the high flows.   Secchi

                                  80

-------
  disk measurements are lower during that period than  any  other  time
  during the year and a large fraction  of the  material  caught  in sediment
  traps is  clay,  which together reflect the  large contribution from land
  runoff.
       Additionally,  early  spring  blooms  of  diatoms remove P from the
  epilimnion more effectively than non-diatoms.   Years  in which early
  diatom blooms occur also  have lower spring and  summer mean P concentra-
  tions  in  the photic zone.   This mechanism  is not contributing to mainte-
  nance  of  the wintertime 30  yg P/l  residual, but may contribute to resis-
  tance  to  increased  P  income of lakes  with low mixing potential.
  Interestingly,  the years with early spring diatom blooms show one-half
  the March  - August  productivity compared to non-early-bloom years.
      Thus,  it appears that  in  the range of P income to Lake Sammamish,
  1.0 g P/m-yr before to 0.67  g P/m -yr after diversion, and assuming no
 change in the sediment release of P, the P content could have remained
 relatively unchanged with only a change in the sedimentation  rate.   In
 further support of this relative stability in that range of P income,
 a modified version of a mass balance model  (Vollenweider, 1969) can  be
 used to predict only a slight change in water column  content  of P (Krull,
 1973; Welch, et al., 1973).   Phosphorus input,  outflow,  and exchange with
 the sediments were included  in the  model.   The  lake was assumed to be  two
 layered during  the stratified period and uniformly mixed  during the
 remainder  of the year,  and the water column phosphorus content was con-
 sidered to be influenced only by  the above  mentioned gains and  losses;
 complex cycling  within  the water  column  was ignored.  The model con-
 sidered sedimentation and  release rate separately.  The release rate was
 assumed  constant,  while the  sedimentation rate was not held constant.
 This was the key departure from the Vollenweider model (Vollenweider,
 1969) which earlier  had shown  that Lake Sammamish P should exhibit a
prompt and  significant recovery (Emery, et al., 1973).  The modified
model indicated  only a slight  change (7 percent) in phosphorus concen-
tration to a one-third reduction in phosphorus loading.
                                 81

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     Although the above model  allowed the sedimentation rate to vary,
the sedimentation rate varied proportional  to the phosphorus concen-
tration in the water column.  Therefore, as an another approach to
realistically reflect the lake dynamics the sedimentation rate Rock
(1974) allowed to vary with the phosphorus loading to the lake.  The
second modified model was;

              dP = M_|yp.nc
              dt   A   V   R   S

where,
                                     M
         S = sedimentation  rate = K, ^
         P = total phosphorus  in the water column
         R = release  rate
         M = input loading  rate
         A = surface  area of  lake
          KQ  = output  rate coefficient
          K,  = sedimentation rate coefficient
      The  release rate was assumed constant.   Data  showed that the winter
 column concentration  of total  phosphorus was unchanged from pre-diver-
 sion to post-diversion.   Under these conditions  the model  could be
 calibrated.   At pre-diversion steady state;
 where,
               D = retention rate of phosphorus in lake,
 thus, equation (1) becomes;
               D = S - R                              (2)
 and for pre-diversion lake conditions equation (2) becomes;

             20,000 - 3,300 kg = 20.000 kg . K  _ R
                19,8 km2          19.8 km2    ]
                                  82

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 at post-diversion steady state, equation (2) becomes;

       13.000 - 3,300 kg = 13.000 kg .  K  _ R              (4)
           19.8 km2        19.8 km2      ]
 equations (3) and (4) were solved by simultaneous equations, hence
 K  = 0.96.
      Solution of equation (1) yields the change in steady state phos-
 phorus concentration (AP) for a given step change in loading (AM)

              P=f  (1 -K,)                              (5)

      The solution of equation (5)  for the 1968 sewage diversion (AM  =
 7000 kg  P) with K]  = 0.96 gives a   P of only 0.014 g/m2 in the water
 column.   This represents a concentration change in the water column  of
 only 0.5 yg P/l  (^2%).   Thus, the  model  indicates that if a decrease
 in  loading was  followed by a  decrease in sedimentation rate,  the water
 column phosphorus concentration should not  have been significantly
 reduced.
 IMPLICATION OF  NON-RECOVERY
      Although non-recovery would suggest that  the sewage  diversion pro-
 ject  was  a failure,  such a conclusion  is  completely  unwarranted.  The
 primary objective of the project was  to  stop the  cultural  eutrophication
 of Lake Sammamish.   Although  the trophic  state  in  terms of  lake concen-
 trations  was  not  reversed, the  present trophic  state does not  represent
 deteriorating nor intolerable conditions.   In fact, the mean summer chl
 a level in Lake Sammamish is now about equal to that found  in  Lake
 Washington following diversion  (Edmondson, 1972).  Further, summer water
 clarity,  as measured by  the Secchi disk has been greater than  3 meters
on the average during all the years investigated, before and after
diversion.  Lake Washington on the other hand showed average summer
Secchi disk depths of only 1  m before diversion (Edmondson, 1972) and
now has similar clarity as Sammamish.
                                 83

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     Although Lake Sammamish did not noticeably  improve  following  diver-
sion of wastewater, the action was  no doubt a  preventative measure.   The
lake may have in fact advanced in trophic status without the 1968  diver-
sion.  The lack of response over the past 100  years to the range of P
                           2
income of 1.0 to 0.67 g P/m .yr tempts one to  speculate  that future in-
crease may also not affect trophic state.  That  is probably not tenable
because although most of the above increase in P probably came in  the last
10 years.  Issaquah, the main source of sewage,  has nearly tripled in
population since 1960 and the wastewater from  the Issaquah area has doubled
since diversion in 1968.  This suggests that the increase in P income to
Lake Sammamish over the past century has been  in the "lag phase" and only
very recently could the increase be described  as entering the "growth
phase11 of an exponential function.  Without diversion, the P income would
not  be 0.67 g P/m  .yr, or 1.0 g P/m2.yr  (the pre-diversion rate),  but
                 2
rather 1.36 g P/m  .yr, which would reflect the recent growth in the
Issaquah area since 1968.  Such an increase would most probably not all
go  into the sediments and the present winter mean content of 31 yg/1 would
surely have increased.  This seems especially reasonable since the iron
supply for complexation would not have increased proportionately.
     Although Lake Sammamish has remained  stable over a range in a P
income of 0.67  to  1.0 g P/m  .yr that  probably has  little implication for
future increases  tn  Income.  The important question now relates to the
expected  increase  from  suburban and  urban  runoff that can be expected as
the forested areas around the  lake,  particularly the east side, are reduced
to  residential  areas.   At first approximation the  yield of  P from  such
developed areas in the  Sammamtsh watershed do not  appear great, but  in
light  of what  has  been  demonstrated  by careful  analysis of  the  P  trans-
port in  Issaquah Creek  (high runoff  rates  yield high  P  concentrations)
the question of yield  increase from residential  development must  receive
more careful study if  a totally reliable prediction  is  to  be  obtained.
      The implication of this lake's  response  to P  income  manipulation
                                 84

-------
 to the management of other lakes is  not entirely  clear.   Certainly one
 is left with the overriding impression that lakes with anaerobic hypo-
 limnia are more unpredictable than those that  remain  aerobic.   If the
 degree of P manipulation in Sammamish  is compared with that of
 Washington on the Vollenweider graph (Figure 27)  it becomes of  course
 clear that the degree of manipulation  was greater in  Washington.  Yet
 from that graph one  would have expected a proportional change in lake
 trophic state, other factors being equal, since the loading in  Sammamish
 was  decreased to a point near the "excessive"  line similar to that in
 Washington.   Obviously such a proportional  response did not occur, thus
 one  must agree with  Vollenweider and Dillon (1974), for anaerobic lakes
 at least,  that P removal  in excess of  50% of the  input is necessary to
 observe a  significant recovery.  In  support of that Larson, et al. (1975)
 have observed an improvement in  the  trophic state  in  Shagawa Lake, which
 has  an  anaerobic hypolimnion,  as a result of an interception of 70% of
 the  P income  (Fig. 27).   However, Shagawa  was very  eutrophic at a
 similar loading  as Sammamish (Malueg,  et al., 1973).  Clearly more of
 the  incoming  P in  Lakes  Washington and Shagawa ended  up in the water
 column  and  available  to  algae  than was  the  case in Lake Sammamish.  The
waste water P  entered  Lakes  Washington  and  Shagawa directly while
 in Sammamish  the waste water entered through Issaquah Creek.   This
 could be at least part of the  reason why much of the loaded P to
Sammamish does not end up in the water column but rather in the sedi-
ment.   Issaquah Creek carries a rather high load of iron so possibly
much more of the P added to  Issaquah  Creek was  destined for the sedi-
ments of Lake  Sammamish than was the  case for the waste water P added
to the other two example lakes.
                                 85

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oo

1.0 -
•
\J
2:
OJ
c
(Q
a
_i
to
i o.,-
CO
o
C"
-_*-_
Q.
2
n.m -
Eutrophic ^
^Washington 1964 ^
Mesotrophic i ^^
Sammamish 1965 £ i Mesotrophic ^s'
| 0Washj*x^ comparing the relative
position of three manipu-
lated lakes. Relationship
after Vollenweider (1974).
                 0.1
1.0
10.0
100
                                                      Z/TW (Meters/year)

-------
 CONTRIBUTION OF THE  MODEL  TO UNDERSTANDING
      As  the  results  have shown,  the model has  provided some interesting
 information  as  well  as  raising questions which have provided impetus and
 direction  for new  or more  thorough research  in certain critical areas.
 As with  any  modeling effort  its  major contributions are as a test vehi-
 cle  for  new  hypotheses  and as a  source of direction for research which
 will  expand  understanding.
      This model  has  helped to increase understanding in such areas as
 the  importance  of  mixing of  Lake Sammamish waters to algal growth, the
 effect of light intensity  on algal growth, the relative importance of
 diffusion in  the water  column versus external  inputs of nutrients and
 effects of wind and  insolation upon themixed depth of the lake.
      Interesting questions have been raised as to littoral and bottom
 release as contributors to the PO^-P pool as well as the effects of
 surface runoff and atmospheric fallout.   The model is available for use
 in testing any new theories which are generated in these or other areas
of interest using Lake Samamish as the study site.
                                87

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                         SECTION 10

                         REFERENCES

American Public Health Association, and others, "Standard methods
for the examination of water and wastewater," 13th ed.,  APHA, New
York (1971).

Birch, P., "Sedimentation in lakes of the Lake Washington Drainage
Basin," M. S. Thesis.  Univ. of Wash. (1974).

Birch, P. B., "The Relationship of Sedimentation and Nutrient Cycling
to the Trophic Status of Four Lakes in the Lake Washington Drainage
Basin," Ph.D. dissertation, Univ. of Wash. (1976).

Bremner, J. M., "Determination of Nitrogen in Soil by the Kjeldahl
Method," J. Agri. Sci. 52_, pp. 137-146 (I960).

Edmondson, W. T., "Nutrients and Phytoplankton in Lake Washington,"
In  Nutrients and_Eutrgphication - Special Symposium, Limnol. and
Oceanog., 1, pp. 172-193 (1972).

Emery, R. M., "Initial responses of phytoplankton and related factors
in Lake Sammamish following nutrient diversion," Ph.D. dissertation,
Univ. of Wash.  (1972).

Emery, R. M., Welch,  E.  B., and Moon, C.  E.,  "Delayed Recovery of a
Mesotrophic  Lake after Nutrient Diversion,"  J. Water Pollut. Cont.
Fed., 45, pp. 913-925 (1973).

Fish, E.,  "The  past  and  present in Issaquah,  Washington," (1967).

Gibbs, C,,  Henry, C., and  Kersnar, F., "How  Seattle beat  pollution,"
Water and Wastes Engineering,  9^ pp. 30-40  (1972).

Guttormsen,  S.,  "A comprehensive nitrogen study of Lake  Sammamish,"
M. S. Thesis, Univ.  of Wash.  (1974).

Hansen,  H.,  "Postglacial forest  succession and climate  in the Puget
Sound  region,"  Ecology,  19^  pp.  528-542  (1938).

Hendrey,  G., "Productivity and growth  kinetics of natural phyto-
plankton  communities in  four lakes of  contrasting trophic state,"
Ph.D.  dissertation,  Univ.  of Wash.  (1973).

Horton,  M., "The chemistry of P  in Lake  Sammamish," M.  S. Thesis.
Univ.  of Wash.  (1972).

 Isaac,  G.  W., Matsuda, R.  I., and  Walker, J. R.,  "A limnological
 investigation of water quality conditions in Lake Sammamish," Water
 Quality Series  No.  2., Municipality  of METRO Seattle  (1966).

-------
  Kemmerer,  G.,  Bovard,  J.,  and  Boorman, W.,  "Northwestern  lakes of
  the  U.S.:   Biological  and  chemical  studies  with reference to pos-
  sibilities  in production  of fish,"  Bull. U.S. Bur. Fish., 39,
  pp.  51-140 (1923).

  Krull, J.,  "Phosphorus response model for Lake Sammamish," Unpub.
 manuscript,  Univ. of Wash.  (1973).

 Larson, D.  P., ejt al_., "Response of  Eutrophic Shagawa Lake, Minne-
 sota, U.S.A.,  to Point-source, Phosphorus Reduction," Verh. Intl.
 Ver. Llmnol. 19., pp. 884-892 (1975).

 Libby, R. A.,  "The application of Newton Activation Analysis to
 Water Samples," M. S. Thesis, Dept. of Nuclear Engineering, Univ.
 of Wash. (1972).

 Liesch, Price and Walters, "Geology and groundwater resources of
 Northwest King County,  Washington," Water Supply Bulletin No. 20.
 USGS (1963).

 Livingston, Jr.,  V., "Geology and mineral resources of King County,
 Washington," Wash.  Dept.  of Nat.  Resources Bull.  No.  63 (1971).

 Lorenzen,  M. W.,  "Predicting the  effects  of nutrient diversion  on
 lake recovery," In  Modeling the Eutrophication Process.  Middlebrooks,
 J.  E.,  et  al_. (Eds.) Utah State Univ., pp.  205-210 (1973).

 Malueg,  K.  W.,  et aK,  "The Shagawa  Lake  Project;  Lake Restoration
 by  Nutrient Removal  from  Wastewater  Effluent," Environmental  Protec-
 tion  Agency, Corvallis, Ore., 49  pp.  (1973).

 McDonnell,  J. C., "In Situ  Phosphorus Release  Rates from Anaerobic
 Lake  Sediments,"  M.  S.  Thesis,  Univ.  of Wash.  (1975).

 Monahan, F.,  "An  In  Situ  study  of sediment nutrient release in Lake
 Sammamish,"  M.  S. Thesis". Univ. of Wash.  (1974).

 Moon, C. E.,  "Nutrient  budget following waste  diversion  from a meso-
 trophic lake,"  M.S. Thesis.  Univ. of  Wash.  (1973).

 Noah, C. M.,  "A Multiparametric Model  of the Nitrogen System in
 Lake Sammamish," M.S. Thesis, Univ. of Wash. (1976).

Pederson, G. L., "Zooplankton Population Dynamics and Production
 in Three Lakes of Contrasting Trophic Status," Ph.D. dissertation,
Univ.  of Wash. (1974).
                               89

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28.  Rock, C. A., "The trophic status of Lake Sammamish and its relation-
     ship to nutrient income," Ph.D.  dissertation,  Univ.  of Wash.,  (1974).

29.  Shapiro, J., Edmondson, W. T.  and Allison, D.  E., "Changes in  the
     chemical composition of sediments of Lake Washington, 1958-1970,"
     Limnol. Oceanog. Ijj, pp. 437-452 (1971).

30.  Stockner, J., "Preliminary characterization of lakes in the Experi-
     mental Lakes Area, Northwestern Ontario, using diatom occurrences
     in sediments," J. Fish. Res. Bd. Canada, 28, pp.  265-272 (1971).

31.  Strickland, J. D., and Parsons, T. R., "A practical handbook of
     seawater analysis," Bull. Fish.  Res. Bd. Canada.  No. 167 (1968).

32.  Strutevant, P., "Growth Rate Parameters and Biomass Measurement
     Ratio for Natural Algal Populations," M. S. Thesis, Univ. of Wash.
     (1974).

33.  Swayne, M., "Enviornmental monitoring from a communication engineer-
     ing point of view," M. S. Thesis.  Univ. of Wash. (1973).

34.  Tang, C. H., "A multiparametric lake model," Ph.D. Dissertation,
     Univ. of Wash.  (1975).

35.  Vollenweider, R. A., "Possibilities and limits of elementary models
     concerning  the  budget  of  substances in  lakes," Arch. Hvdro1obiology_»
     66,  pp.  1-36  (1969).                           	        "

36.  Vollenweider, R. A. and  Dillon, P. J.,  "The application  of phos-
     phorus  loading  concept to eutrophication  research,"  N.R.C. Tech.
     Rep.  13690, 42  pp.  (1974).

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     Process.  Middlebrooks, J. E., et  al.  (Eds.),  Utah  State  Univ.,
     pp.  5-14 (1973).              	

38.  Welch,  E.  B., Hendrey, G. R., and Stoll,  R. K.,  "Nutrient Supply
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     Oikos,  26., pp.  47-54  (1975).

39.  Wiederholm, T., "A survey of  the bottom fauna of Lake Sammamish,11
     Northwest Science. 50, pp.  23-31  (1976).
                                  90

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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO. 2.
EPA-600/3-77-003
4. TITLE AND SUBTITLE
Nutrient Diversion: Resulting Lake Trophic State
and Phosphorus Dynamics
7. AUTHOR(S)
Eugene B. Welch
9. PERFORMING ORGANIZATION NAME AND ADDRESS
University of Washington
Seattle, Washington 98195
12. SPONSORING AGENCY NAME AND ADDRESS
U.S. Environmental Protection Agency
Con/all is Environmental Research Laboratory
200 S.W. 35th Street
Con/all is, Oregon 97330
3. RECIPIENT'S ACCESSION-NO.
5. REPORT DATE
January 1977
6. PERFORMING ORGANIZATION CODE
8. PERFORMING ORGANIZATION REPORT NO.
10. PROGRAM ELEMENT NO.
1 BA031
11. CONTRACT/GRANT NO.
EPA R-800512
13- Bar -E?ffi m% i WTRED
14. SPONSORING AGENCY CODE
EPA - ORD
15. SUPPLEMENTARY NOTES
lb. ABSTRACT
Lake Sammamish, Washington, was studied during 1970-75 to determine its
           LaKe bammamisn, wasmngton,  was suuuicu uU( ius  «•"<•• •-  —	•	:~.
     response to wastewater diversion  in  1968.   The results were  compared with  a
     pre-dtversTon study in 1964-65.   Diversion Reduced the phosphorus  loading
     by about one-third (from 1.02  to  0.67 g P/nT. yr and about 119 to  68 p  g/1  in
     the  inflow).  Winter total phosphorus remained constant and  no trend was shown
     tn chlorophyll  a in spring-summer.   Water  transparency remained the same.
           PaleolimnoTogical evidence suggests that the lake has been near its present
     mesotrophic state for about 100 years.   This stability is thought  to be due  to
     the  constancy of the water phosphorus concentration which is in turn controlled
     by the  anaerobic-aerobic release  and sedimentation of iron and its complexes.
           Although the effect of a  one-third reduction in phosphorus Joading.as  well
     as a  similar increase resulting from the urbanization impact of the 1960 s,  was
     not  apparent in the lake, without diversion loading would now be increased to
     1.36  g  P/m2. yr.
 7.
KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
     Lakes *
     Limnology
     Phosphorus  *
     Algae
     Eutrophicatton  *
     Trophic Load                     .
           (*denotes major descriptors;
                                              b.lDENTIFIERS/OPEN ENDED TERMS
                                         :.  COSATI Field/Group
                                              02H
                                              04A
                                              o5C
IS. DISTRIBUTION STATEMENT

     Release unlimited
              19. SECURITY CLASS (ThisReport)
                  Unclassified
21. NO. OF PAGES
  100
              20. SECURITY CLASS (Thispage)
                  Unclassified
                                        22, PRICE
EPA Form 2220-1 (0-73)
            91
                           ft U. S. GOVERNMENT PRINTING OFFICE: 1976-796-587 / 27 REGION 10

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