United States
          Environmental Protection
          Agency
          Industrial1 Environmental Research
          Laboratory
          Cincinnati OH 45268
EPA-600/7-78-109
June 1978
          Research and Development
&EPA
Response of a Salt
Marsh to Oil Spill
and Cleanup:
Biotic and
Erosion a I Effects
in the Hackensack
Meadowlands,
New Jersey

Interagency
Energy/Environment
R&D Program
Report

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                 RESEARCH REPORTING SERIES

 Research reports of the Office of Research and Development, U.S. Environmental
 Protection Agency, have been grouped into nine series. These nine broad cate-
 gories were established to facilitate further development and application of en-
 vironmental technology. Elimination  of  traditional grouping was  consciously
 planned to foster technology transfer and a maximum interface in related fields.
 The nine series are:

      1.  Environmental Health Effects Research
      2.  Environmental Protection Technology
      3.  Ecological Research
      4.  Environmental Monitoring
      5.  Socioeconomic  Environmental Studies
      6.  Scientific and Technical Assessment Reports (STAR)
      7.  Interagency Energy-Environment Research and Development
      8.  "Special"  Reports
      9.  Miscellaneous Reports

 This report has been assigned to the INTERAGENCY ENERGY-ENVIRONMENT
 RESEARCH AND  DEVELOPMENT series. Reports in this series result from the
 effort funded  under  the 17-agency Federal Energy/Environment Research and
 Development Program. These studies relate to EPA's mission to protect the public
 health and welfare from  adverse effects of pollutants associated with energy sys-
 tems. The goal of the Program is to assure the rapid development of domestic
 energy supplies in an environmentally-compatible manner by providing the nec-
 essary environmental data and control technology. Investigations include analy-
 ses of the transport of energy-related  pollutants and their health and ecological
 effects; assessments of, and development of, control technologies for energy
 systems; and  integrated assessments of a wide range of energy-related environ-
 mental  issues.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                                EPA-600/7-78-109
                                                June  1978
RESPONSE OF A SALT MARSH TO OIL SPILL AND CLEANUP

       Biotic and Erosional Effects in the
       Hackensack Meadowlands, New Jersey
                        by

                 Phillip C. Dibner
                    URS Company
            San Mateo, California  94402
            Contract Number 68-03-2160
                 Project Officer

               Leo T. McCarthy, Jr.
     Oil and Hazardous Materials Spills Branch
   Industrial Environmental Research Laboratory
             Edison, New Jersey  08817
    INDUSTRIAL ENVIRONMENTAL RESEARCH LABORATORY
        OFFICE OF  RESEARCH AND DEVELOPMENT
       U.S.  ENVIRONMENTAL PROTECTION AGENCY
             CINCINNATI, OHIO  45268

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                                 DISCLAIMER


     This report has been reviewed by the Industrial Environmental Research
Laboratory-Cincinnati, U. S. Environmental Protection Agency, and approved
for publication.  Approval does not signify that the contents necessarily
reflect the views and policies of the 0. S. Environmental Protection Agency,
nor does mention of trade names or commercial  products constitute endorsement
or recommendation for use.
                                     ii

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                                  FOREWORD


     When energy and material resources are extracted, processed, converted,
and used, the related pollutional impacts on our environment and even on our
health often require that new and increasingly more efficient pollution
control methods be used.   The Industrial Environmental Research Laboratory -
Cincinnati (IERL - Ci) assists in developing and demonstrating new and
improved methodologies that will  meet these needs both efficiently and
economically.

     This study consists of an assessment of the biological and erosional
effects of a crude oil spill in the Hackensack River, New Jersey, and sub-
sequent cleanup operations.   The information gained as a result of this
experience will be valuable to oil spill onscene coordinators when planning
and responding to future spills under similar environmental conditions.
Personnel responsible for future damage assessment surveys should also find
the report useful.  For further information, please contact the Oil and
Hazardous Spills Branch of the Resource  Extraction and Handling Division.

                                     David G. Stephan
                                         Director
                       Industrial Environmental Research Laboratory
                                        Cincinnati

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                                  ABSTRACT


     This study addresses the biological and erosional response of portions
of the Hackensack Meadow!ands estuarine marsh to the Well en Oil Company
number 6 crude oil spill of late May 1976, and the subsequent cleanup opera-
tions.  Cleanup included cutting and removal of oiled grasses of the species
Spartina al term'flora from the bank of the Hackensack River.   Data were
gathered from several locations along the riverbank and in the inner marsh
during four sampling sessions, at approximately 4-month intervals, throughout
the year following the spill.   The productivity of the marsh plants, the
composition of marsh soil invertebrate communities, the presence of oil in
the substrate, and erosional  trends were monitored.  Results suggest that
cutting heavily oiled Spartina soon after contamination saved the plants from
dying by root suffocation.However, the foot traffic associated with cutting
is implicated as having made the river bank susceptible to severe erosion by
boat wakes and other sources of erosive energy.   It is concluded that cutting
is only desirable in a limited range of circumstances, determined by the
characteristics of the contaminating oil, the biology of affected plants, and
the time of year.
                                     iv

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                                  CONTENTS


Section                                                                  Page

          FOREWORD	     iii

          ABSTRACT	      iv

          FIGURES	     vi i

          TABLES	    vi ii

          ACKNOWLEDGEMENT	     ix

   1      INTRODUCTION 	      1

          Description of the Study Area	      1
          Oil Spill Cleanup History	      4
            Spill Incident	      4
            Marsh Cutting Operations 	      6

   2      CONCLUSIONS	     10

   3      RECOMMENDATIONS	     11

   4      STUDY RATIONALE AND METHODOLOGY	     13

          Potential Impacts of Spill and  Cleanup  	     13
            Effects of Oil	     13
            Effects of Cutting	     13
            Synergistic Effects	     14
          Rationale for Data Collection	     14
            Vegetation	    15
            Soil Fauna	    15
            Oil in Substrate	    15
            Erosion	    15
          Location of Sample Stations	    16
            Physical and Biological Attributes  of the
              Sample Stations	,	    16
          Sampling Procedure 	    19
            Vegetational Distribution  and Productivity 	    19
            Fauna	    21
            Distribution of Oil  in the Substrate	    22
            Erosion	    22

    5      RESULTS	    23

          Vegetation	    23
          Fauna	    26
          Oil  in  the  Substrate	    26
          Erosion	    27

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                              CONTENTS (cont.)


Section                                                                  page

   6      DISCUSSION	      29

          Sources of Error	      29
          Vegetation	      31
          Fauna	      32
          Oil  in Substrate	      33
          Erosion	      34

          REFERENCES	      36

          APPENDIX	      38
                                    vi

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                                   FIGURES
Number
                                                                          o
  1       Study Area ..........................
  2       Spartina al term' flora ....................    3
                                                                          c
  3       Phragmites commums .....................
  4       Contaminated Regions in the Hackensack Meadowlands ......    7
  5       Cut Area on Hackensack River Bank ..............    8
  6       Location of Sampling Stations ................    17
  7       Configuration of Vegetational Sampling Stations .......    20
  8       Total  Plant Volume Per Unit Area ...............    24
  9       Position  of Bank Edge vs. Time ................    28
  A-l     Percent Cover  ........................    4^
  A-2     Density ...........................    42
  A-3     Total  Plant Volume  Per Unit  Area ...............   43
  A-4     Height ............................   44
  A-5     Diameter ...........................   45
  A-6      Individual Stem Volume ....................   46
  A-7      Position of Bank Edge vs.  Time ................   51
                                       vii

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                                   TABLES
Number                                                                   Page
  1       Summary of Sample Stations 	     18
 A-l      Measurements of Phragmites 	     38
 A-2      Measurements of Spartina	     39
 A-3      Marsh Soil Invertebrate Populations	     47
 A-4      Marsh Soil Fauna:   Number of Invertebrate Genera
            and Individuals,  and Invertebrate Diversity Index	     48
 A-5      Observed Changes in Soil  Surface Elevation at
            Sedimentation Stakes 	     49
 A-6      Observed Changes in Position of Shoreline
            Break-in-slope 	     50
                                     viii

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                               ACKNOWLEDGMENTS


     Sincere thanks are extended to Chester Mattson, Donald Smith,  Nicholas
Vallario and other members of the Hackensack Meadowlands Development Commis-
sion who permitted this research to be performed in their jurisdictional
area.  Mr. Smith was particularly helpful in providing information about the
spill and helping the research team become familiar with the area.

     Robert Castle was responsible in large part for the sampling design.  He
also provided information and prepared much of the text on erosional pro-
cesses.   Leon Grain performed the onsite observations of the oil spill
cleanup operations and contributed most of the text that describes them.

     Michael Slack and Diane Renshaw edited the manuscript and provided many
helpful suggestions.  Susan Samse prepared the graphics, Diane Renshaw pro-
vided the botanical line drawings, and Margaret Chatham and Donna Murzi typed
and  produced the  final report.  The study was conducted under the Oil and
Hazardous Materials Program of URS Company; Bill Van Horn, program manager.

     Special thanks are due to Leo McCarthy and Stephen Dorrler of the
Environmental Protection Agency, without whose support this work would not
have been possible.
                                       ix

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                                  SECTION 1

                                INTRODUCTION


     This study addresses the response of salt marsh communities in  the
Hackensack Meadowlands, New Jersey (Figure 1), to an oil  spill  that  occurred
in late May 1976.   A primary objective of the study was to develop recommen-
dations for minimizing the adverse biological and erosional  impacts  of clean-
up operations in future spills.

DESCRIPTION OF THE STUDY AREA

     The Hackensack Meadowlands comprise a great complex of estuarine marshes
in northern New Jersey, across the Hudson River from New York City.   Although
most of the surrounding region has been built on to provide industrial and
residential facilities, approximately 6,300 acres of water and marshland
remain essentially undeveloped (Mattson and Vallario, 1976).  The distin-
guishing characteristics of this undeveloped region are described below.

     The Hackensack River flows through the marshes, carrying nutrient-rich
sediments from inland.  As the river slows on its approach to the sea, it
deposits a portion of these sediments.  Some of these, as well as materials
contained in industrial and sanitary effluents from the towns and cities
nearby, become incorporated into the marsh soil and feed the plants  growing
there.

     Organic materials in the sediment, along with the remains of dying
plants, are decomposed by bacteria.  The decomposition process requires more
oxygen than is available in the benthic environment.  The marsh soil  becomes
anaerobic, and the dead stems of marsh grasses only partially decay.  The
partly decomposed plant material accumulates after each growing season and
adds to the peat substrate.

     The Atlantic Ocean also exerts a considerable influence on the marsh
ecosystem.  Tidal waters move upriver twice  daily.  They run over the soil
and flush away wastes.  The mixture of sea and river water produces a
brackish-water environment that eliminates competition from salt-intolerant
species, and allows the salt-adapted marsh grasses to thrive.

     The naturally dominant plants of saline marshes  in this portion  of the
United States belong  to the genus  Spartina.  One  species, Spartina patens,
grows in the higher portions of marsh and  is of no concern  in this study.
However, the salt marsh cord grass, Spartina alterniflora,  is of primary
importance in the Hackensack Meadowlands and was  therefore  the object of
considerable observation during this  investigation.   This species is  depicted
in Figure 2.  Spartina  inhabits the frequently inundated zones  adjacent to

                                      1

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                -\~  * - _*
Source:  ll.S.G.S.  (1967 a & b).
                             Figure 1.   Study Area

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                                           0.5 meter
Figure 2.  Spartlna alternlflora

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 creeks  and channels  in the  marsh.*   It has  colonized  much  of  the  broad  ex-
 panse of mudflats  in the  inner  or back marsh  of  the Hackensack  Meadowlands,
 away from the main river  channel.   In  the downriver portions  of the  Meadow-
 lands,  Spartina alterniflora  populates the  banks of the  Hackensack River.

      Although the  leafy tissues and aerial  stems of the  Spartina  plant  die
 back each year,  the  underground stems, or rhizomes, and  roots are perennial
 structures.   These organs,  which are essential to the plant's vegetative
 survival, require  oxygen  to survive, but cannot  obtain it  from  the oxygen-
 poor soils in which  the plant commonly grows.  They obtain necessary oxygen
 and  release carbon dioxide  waste through a  system of  open  spaces  and hollow,
 air-filled tubes that open  to the atmosphere  through  pores in the leaves,
 called  stomata.  Specially  adapted  guard cells close  when  the leaves are
 inundated, to prevent the air spaces from filling with water  (Teal and  Teal,
 1969).

      Spartina is a salt-tolerant plant.  It actually  grows quite  vigorously
 in freshwater environments, but is  infrequently  observed there  because  it
 cannot  compete successfully with species that are specifically  adapted  for
 living  in freshwater marshes  (Teal  and Teal,  1969).

      One such freshwater  plant  is Phragmites communis, a common reed that
 grows in a wide  range of  habitats.   Phragmites.  shown  in Figure 3, is another
 dominant plant of  the Hackensack Meadowlands, and the  only species besides
 Spartina alterniflora that  received  intensive study in the present investi-
 gation.   PhragmitesTcan tolerate dry ground.  It  grows on  the higher banks
 and  remains  of old dikes.   It also  is  the dominant species in much of the
 marshland in  the northern portion of the study area.

      Just as  Spartina grows well in  freshwater,  so can Phragmites tolerate a
 certain  amount of  salt.   Its presence  in the upper limits  of  salt marshes is
 fairly common, but it does  not  grow  well when the  salt concentration (chlo-
 ride) in the  soil  water exceeds  about  1.2 percent  (Haslam,  1972).   Phragmites
 also  seems to  be similar to Spartina in the way  its roots  obtain  oxygen.
 They  contain  some  air-filled tissue  and do  not seem to be  starved for oxygen
 when  the plant bases  are  inundated (Buttery et al., 1965).

      Interested  readers are referred to a biological inventory of the marshes
 and waterways  in the  Meadowlands compiled by the  Hackensack Meadowlands
 Development Commission  (1975),  for more detailed  information on the  study
 area.

OIL SPILL  CLEANUP HISTORY

Spill Incident

     On  the night of May 25-26,  1976, at the site of the Wellen Oil   Company
storage  facility in Jersey City, New Jersey, an oil tank ruptured.  The

*The  term Spartina  alone will  refer from here on to the  species Spartina
 alterm'flora, unless specific reference is  made to the  genus at large or
 to  some other species.

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                                        1 meter
Figure 3.  Phragmltes conrounls

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 containment berm surrounding the tank failed,  resulting in the release of
 approximately 2 million gallons of No.  6 crude oil  into the Hackensack River.
 The flood tide carried the oil  approximately 3.7 kilometers upriver into the
 vicinity of the Kingsland Creek-Sawmill  Creek area  of the Hackensack Meadow-
 lands.   A strong east wind directed the  bulk of the oil to the west bank of
 the river.   Currents of more than 2 meters per second (4 knots) in conjunc-
 tion with the wind threatened to carry  the oil up several small channels into
 the interior of the marsh.   Booms, placed across the entrances to the most
 important channels, failed against the  strong  currents.  As a result, most of
 the back marsh and mudflat areas were exposed  to the spilled oil.   The most
 severely affected regions are shown on  the map in Figure 4.   The viscous oil
 adhered to most plants and yegetational  debris within the tidal range.   Much
 of the  contaminated vegetation  in the marsh interior, however, was situated
 in the  path of tidal  currents,  which washed some of the oil  from the plants.
 The pattern of soil contamination was less apparent.   In some locations,  it
 seemed  that only the less water-saturated sediment  permitted adhesion;  else-
 where,  all  the substrate was contaminated.   The large mudflat area in the
 vicinity of Sawmill Creek was exposed to a great quantity of oil.   The oil
 did not strongly adhere to the  substrate and was ultimately removed by tidal
 flushing.

 Marsh Cutting Operations

      After  most of the mobile oil  on the Hackensack River was contained or
 removed by  cleanup contractors,  local, governmental,  and private experts
 decided that cutting  was a  viable  method of removing  the vegetation that had
 been contaminated and which continued to release oil  to the  waters.   On
 June 13,  1977,  17 days after the  spill,  the cutting operation was  started by
 Disch Construction Company.   The  operation took place on the western bank of
 the Hackensack River  from the New  Jersey Turnpike Bridge to  approximately
 0.5 kilometers  north  of Kingsland  Creek,  a total  distance of 2.4 kilometers.
 The cut area,  shown in Figure 5,  included the  most  heavily contaminated
 vegetation.   A horizontal path of  marsh  plants,  approximately 3 meters  wide,
 was cut and removed from the  site.   The  marsh  plants  were cut 5 to 15 centi-
 meters  from the  soil  surface.  Cutting was  terminated on June 30.

      The  cutting  operation  was carried out  only  during  daylight hours.   Marsh
 cutting could  only  take  place during  the  lower  tidal  cycle because at other
 times the Hackensack  River  bank was  underwater,  making  cutting difficult  and
 dangerous.   As a  result,  crews worked 8  to  10  hours each  day.

     One  crew was  employed  in the  cutting  operation.  This seven-man  crew
 consisted of one  foreman, one cutter, and  five plant  debris  handlers.   The
work crew were provided with  one 16-foot motorboat,  2  flat-bottomed  boats,
2 scythes,  and 7 pitchforks and clamforks.  This crew cut  and  removed plant
debris  at an average  rate of 140 linear meters of river  bank  shoreline  per
day.

     The cutter used an aluminum handled brush scythe with a 46-centimeter
blade.  After 10 to 15 minutes of cutting, the scythe blade became dull and

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                                               Contamination
                                                   Light
Subsequent Contamination
 Figure 4.  Contaminated Regions in the Hackensack Meadowlands

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                 -Lltt
                    Hm
mile
 i—
                                       .




               -,^- ^4^. •  .NX i-v.^feSMs
  Figure 5.  Cut Area on Hackensack River Bank

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required sharpening by file.  One cutter was able to keep ahead of the
5 people raking and loading the plants into the flat-bottomed boats.

     Three people were employed in raking the cut vegetation into small
stacks on the river bank.  About 6 to 10 of these stacks would accumulate on
the shoreline prior to removal.  Since protective ground cover beneath the
stacked plants was not employed, oil was able to drain from the stacks onto
the soil.  On occasion stacks were left overnight, allowing oil to leach from
these stacks and be washed away at high tide.  Protective booms, which might
have contained the oil, were not used.

     Two people using pitchforks transferred the plant material from the
stacks to the two flat-bottomed boats.  The flat-bottomed boats were used as
barges to transport the cut plants to an interim storage area beneath the New
Jersey Turnpike Bridge.  At the storage area, neither protective ground cover
on the shoreline and embankment nor protective booming of the shoreline were
observed.  Some of the debris was left at this site until November.   The
plants were subsequently removed to a landfill site that had been approved by
the State of New Jersey.

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                                  SECTION 2

                                 CONCLUSIONS
     The following conclusions are suggested by the observational and suppor-
ting quantitative data gathered during this study:

     1.  Damage to Spartina alterm'flora plants caused by the Well en oil
spill resulted primarily from the physical, not the chemical (toxic) proper-
ties of the oil.  Oil coated the plants, prevented gaseous exchange with the
atmosphere, and ultimately caused the plants'  roots and rhizomes to suf-
focate.

     2.  Mortality was highest in heavily oiled Spartina al term'flora plants
that were neither washed clean by tides nor cut.

     3.  Other oiled plants, which were less heavily oiled or naturally
flushed clean, may have sustained some damage, as evidenced by decreased pro-
ductivity.   However, this result may be an artifact of the sampling procedure
and the distribution of the plants.

     4.  The success of cutting heavily oiled plants as a technique for
reducing long-term damage to them depends upon a combination of factors,
including the biology of the contaminated species, the elapsed time between
contamination and cutting, the season in which cutting is performed, and the
characteristics of the oil.

     5.  Cutting soon after contamination was beneficial.  This treatment
apparently reduced long-term damage to the most heavily oiled marsh grasses
of the species Spartina alterm'flora, despite the fact that it entailed
trampling them.  The foot traffic itself was detrimental, in that it appar-
ently contributed to severe bank erosion.   The heavily trampled portions of
the river bank became more susceptible to erosion by boat wakes and natural
wave and current action.   It cannot be concluded that foot traffic and
cutting were sufficient in themselves to cause severe erosion.
                                     10

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                                  SECTION 3

                               RECOMMENDATIONS


     The following recommendations are based upon observations made in the
course of this investigation.   They constitute an approach to marsh cleanup
that will minimize the potential for damage to marsh plants and to the af-
fected shorelines.  Where economic or other necessity dictates the use of
cleanup procedures that may be more damaging to the marsh than those de-
scribed below, the recommendations provided here may still help to establish
priorities and minimize the adverse impacts of cleanup.

     It should be noted that these recommendations are applicable only where
the contaminating oil is relatively viscous and functionally nontoxic.  Most
heavy fuel oils and some weathered crudes fall within this class.  Diesel and
other light fuel oils do not.   These recommendations are also primarily
applicable to estuaries on the northeastern Atlantic coastline of the United
States.  However, they are likely to be of some use wherever Spartina
alterm'flora and Phragmites communis are dominant components of an oil-
contaminated salt marsh ecosystem.

     1.   As soon as possible after contamination, an oil spill response team
          should determine which portions of marsh are so heavily oiled that
          root suffocation of marsh grasses is likely to occur.  Local or
          regional experts are best relied upon to provide this information.
          If such persons are not available, it may be necessary to use the
          general rule that completely oil-coated plants are in danger of
          suffocating, while plants with any appreciable portion of their
          green tissue exposed to the air are less subject to this form of
          mortality.

     2.   Initial cleanup should consist of low-impact methods, such  as
          low-pressure flushing, to remove free oil from the marsh plants and
          substrate.  The heavily oiled plants that are in danger of  dying
          from root suffocation should be treated first.  Some stands of
          marsh grass may be exposed to extensive natural flushing at tidal
          flow and ebb.  This natural cleansing process may eliminate the
          need for flushing by the cleanup crews.

     3.   Immediately after initial cleanup those plants that remain  heavily
          oiled,  and whose roots are still in danger of suffocating must  be
          treated in some way that will expose their internal tissues to  the
          atmosphere.  The most common treatments are cutting and burning.
          Cutting may be an effective treatment for many species, but it  can
          only be recommended for Spartina alterm'flora on the basis  of  this
          study.

                                      11

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     4.   Manual cutting of oiled plants in shoreline areas exposed to high
          wave and current energy should be employed only after other methods
          have been explored and rejected.   Methods that eliminate, or at
          least minimize foot traffic on the river bank are highly preferred.
          Possible alternatives include use of aquatic vegetation cutters and
          controlled burning.

A few additional recommendations, related to but not necessarily arising from
this investigation, are relevant to possible future studies:

     5.   Continue qualitative monitoring of erosion and plant growth along
          the cut and uncut contaminated regions on the Hackensack River
          bank.   This will  help to elucidate the long-term effects of either
          cutting or of taking no action on bank stability.

     6.   Conduct studies that will  determine the suitability of cutting, not
          cutting, or using innovative (no  foot traffic) removal techniques
          for more toxic oils.

     7.   Use the quantitative data provided in this study to aid in the
          sampling design of future studies in the Hackensack Meadowlands or
          similar regions of the northeastern United States.

     8.   Develop contingency  plans  for oil  spills that will  make optimal use
          of all  available  decontamination  facilities and knowledgeable
          persons in the immediate  vicinity and the region at large.
                                    12

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                                  SECTION 4

                       STUDY RATIONALE AND METHODOLOGY


     The stated objective of the investigation described in this  report was
to "conduct a variety of studies designed to supplement, expand,  and  verify
aspects of the ongoing study (EPA 68-03-2160) regarding development of state-
of-the-art procedures for protection,  cleanup, and restoration of marshlands
endangered by oil spills.  Particular  emphasis [was] to be given  to evalua-
tion of the impacts and effectiveness  of the cutting techniques used  in
cleanup" (EPA, 1976).

     To accomplish these goals in the  context of the Well en spill and sub-
sequent contamination of the Hackensack Meadowlands, it was necessary to
determine the effects of the spilled oil on the marsh flora and fauna, the
biotic effects of the cutting operations, and any additional effects  that
might arise when cutting and oiling occur together.

POTENTIAL IMPACTS OF SPILL AND CLEANUP

Effects of Oil

     The direct effects of oil on marsh plants and animals may be physical  or
toxic.  Physical effects result from the viscous and adhesive properties of
oil.  Stomata (gas-exchange pores) of plants may be blocked.  The gills and
mouth parts of invertebrates may become clogged; the fine structure of birds'
feathers may be disrupted, preventing flight and reducing or eliminating the
insulatiye protection that the feathers provide.  Crapp  (1971) has summarized
the physical effects of oils as those that act "by  smothering organisms and
cutting off respiratory exchange, and by interfering with their  movement. .  ."

     Toxic effects result from the oil's interaction with the biochemical
functioning of contaminated organisms.  Baker (1971a)  has discussed a few
hypothetical mechanisms of toxic action on plants.  .The  most general of these
is the replacement of fatty molecules in cellular membranes, resulting in
membrane disruption, increased permeability,  and  leakage of cellular con-
tents.  Increase in  respiration  is often observed,  possibly because mito-
chondria (the organelles responsible  for aerobic  respiration) are  damaged so
that the rate of oxygen  use is  no longer coupled  to other biochemical pro-
cesses.

Effects of Cutting

     Most grasses, including  the familiar  species  used for  lawn  plantings,
are not adversely  affected  by cutting.   This  is because the growth tissue, or


                                      13

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 meristem,  is near the base of the plant.   However,  the response of some
 species may depend upon the time of year,  meteorological  conditions,  and
 tides.   Phragmites communis is one such species.  While cutting may not
 directly injure the plant, tides that submerge the  cut stubble prevent air
 from reaching the roots.   If reduced aeration occurs  in late summer,  it can
 seriously  impair the underground formation of new buds (Haslam,  1970).
 Furthermore, although buds develop throughout the year, they emerge from the
 ground  to  form aerial  shoots only during a relatively brief period in spring.
 They remain dormant throughout the rest of the year unless  the Phragmites
 stand is burned or the rhizomes (underground stems) are cut.   Cutting the
 aerial  stems does not interrupt the buds'  dormancy.   Consequently,  no new
 stems replace those that  have been removed.   If cutting is  performed  directly
 after all  the new shoots  have grown to maturity (e.g.,  in July),  the  plants
 will  be unable to photosynthesize for a large portion of  the growing  season.
 This  can be seriously detrimental  to the stand (Haslam, 1968).

      No such adverse effects of cutting have been reported  for Spartina
 al term flora.   Waisel  (1972) has noted that  repeated  mowing of this species
 has  been observed to stimulate growth and  to promote  uniform growth,  high
 plant density,  and early  flowering.

      Some  methods of cutting vegetation involve heavy traffic on  the  marsh
 soil.   This can result in  trampled buds and  remaining aerial  shoots of  the
 plants,  disrupted substrate,  broken portions of underground stems,  and,
 ultimately,  increased  soil  erosion.

 Synergistic Effects

      Additional  phenomena  not  found in either an  oil  spill  or a cutting
 incident alone  may appear  when  both of these occur  together.   For  example,
 oils  can travel  in the  intercellular spaces  of  uncut  plants,  but they  rarely
 enter the  vascular (liquid  transport)  system.   When plants  are cut, however,
 oil may  enter the  vascular  system  via  the  cut stem, thereby  gaining easier
 access  to  remote  portions  of the plant (Baker,  1971a).

      Another  synergistic effect may occur  if oil  is trampled  into the marsh
 sediments.   These  sediments  are  frequently anaerobic, a condition that  in-
 hibits  the  biochemical degradation  of  oil  (Burns  and  Teal,  1971).   Therefore,
 undecomposed oil  may reside  in the  marsh substrate  for  a  long time.

 RATIONALE  FOR DATA COLLECTION

      Eleven sampling stations were  selected,  and certain  features of each
 station were monitored during four  sample periods.  Data were gathered  on:
 (1) composition and growth of the vegetation, (2) composition of soil in-
 vertebrate populations, (3) presence of oil at various depths in the sedi-
ments, and (4) erosion of the substrate.  The reasons for selecting these
characteristics for sampling are discussed below.
                                     14

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Vegetation

     In any study seeking to examine the implications of a potentially
damaging event on a given ecosystem, first consideration should be given to
the onsite vegetation.   Plants are the only organisms that trap the energy
coming to earth from the sun.   Therefore, all other organisms must ultimately
obtain their energy from plants.   Though few organisms graze directly on
marsh grasses, as the grasses decay they are physically and chemically
reduced to forms that other animals can use.  These animals include shellfish
larvae and fish fry ultimately consumed by humans.   Marsh plants are also of
great importance before they die and decay.  They provide cover for wildlife,
and the cushioning effect of their aerial parts and the binding character of
their roots slow the erosive processes that would wash the marsh sediments
away.

Soil Fauna

     Just as vegetation is an indicator of the quantity of energy available
to the salt marsh ecosystem, so animals can serve as sensitive indicators of
the quality of that system.  Soil animals display different responses to the
presence of foreign materials.  Some benthic organisms, for example, thrive
on the nutrient-enriched sediments near a sewage outfall, while others cannot
survive the high concentration of organic wastes (Patrick, 1950).  Oil pollu-
tion may produce similar results.

     The distribution of species in a sample is as important for providing
insights into the quality of the estuarine environment as are the specific
kinds of animals present.  The few organisms that can live in polluted waters
are likely to be more abundant than any one organism would be where no con-
taminant is present.  The total number of benthic invertebrates  is also an
important indicator of the quality of marsh life because they provide the
food for vertebrates such as fish and wading birds.

Oil in Substrate

     There were two reasons for attempting to determine  if oil were present
in the substrate at each sample station.  The primary reason was to provide
evidence of causality if severe effects on the soil  invertebrates or  the
marsh plants were observed.  The secondary reason was to determine how  long
the oil remained in undegraded form in the substrate.  This would be  parti-
cularly important where the cleanup crews cut the marsh  grasses  and trampled
oil into the soil.   It is generally believed that burial of oil  in anaerobic
substrate prevents or greatly slows its degradation.  The buried oil  can then
exert its detrimental effects on plant roots and benthic invertebrates for a
long time after the original contamination.

Erosion

     The effects of erosion may be more noticeable to people than any other
impact of an oil spill  and cleanup.  Once a river bank erodes, it is no
                                     15

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longer available for colonization by marsh plants.  The biological produc-
tivity that was once associated with the eroded region is permanently lost.
Erosion also adds participate matter to the flowing water, reducing its
quality for use by humans and aquatic organisms.  This particulate material
is likely to be dropped somewhere downstream, aggravating sedimentation
problems and necessitating more frequent dredging, with additional environ-
mental and economic costs.

LOCATION OF SAMPLE STATIONS

     Figure 6 depicts the location of the eleven stations where physical and
biological data were gathered during this investigation.   The sampling sites
were visited four times throughout the year following the spill:   in July
1976, October 1976, March 1977, and July 1977.   A sampling visit was origi-
nally planned for the winter of 1976-1977 but was cancelled because severe
icing would have rendered data collection impossible.

Physical and Biological Attributes of the Sample Stations

     Five sites were selected to evaluate the impact of oil and cutting
operations on the marsh substrate and plants of the river bank.   Stations 1,
2, and 5 were all located directly on the main channel of the Hackensack
River.  Stations 3 and 4 were located respectively on the north and south
banks of a tributary east of the river.   It would have been preferable to
situate all of these stations on the main river channel,  but it was^ecessary
to sample some unoiled areas.  These were found only along tributaries (see
Figure 4).

     The sites for four sampling stations were subjectively selected to be
similar in terms of the elevation and shape of the bank,  which was quite
steep.  Station 1 was positioned in a segment of Phragmites marsh on the
river bank that had been oiled but not cut; it was therefore suitable for
examining the effects of oil contamination in the absence of cutting.
Station 2 was established in a region that had been oiled and cut.  This
station, like the rest of the cut region, supported a mixture of Phragmites
and Spartina.   It was not possible to measure the relative abundance of these
species before cleanup operations were completed.   Station 3, also dominated
by Phragmites. served as an overall control site,  being neither oiled nor
cut.   Finally, the unoiled Phragmites plants at Station 4 were cut by the
research team so that the impacts of cutting could be observed in the absence
of oil contamination.

     At sample station number 5, on the inside of a bend in the river, the
bank assumed the gradual slope characteristic of such locations,  where the
current slows and the water drops its sediment.   This site supported a
heavily oiled stand of Spartina alterniflora. and it was  selected because it
permitted the effects of severe contamination on this prominent marsh plant
to be observed.
                                     16

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                             *
                               ].   I
                                             ... •     - •  , \
   - V. '   -.	- ,
  ~ \> - .-Rad.o T^i^i
                                                       \-- J-'--i' '. a'A       #
                                                    .'--\:;^-  ,  V     /
                                                   I -- . - -z  '.  ' ",-   • >    4 '
                                                               r-i -
Spartina:   Stations 2,5,7,8,9,10
Phragmltes: Stations 1,3,4,
Mudflats:   Stations 6,11
               Figure  6.  Location of Sampling  Stations
                                 17

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     No vegetation was cut in the inner or back marsh of the Kingsland-
Sawmill Creeks area.  Four stations, all dominated by Spartina alterm'flora,
were established there:   two on the east and two on the west side of the New
Jersey Turnpike.   The tidal flows are restricted by the Turnpike; therefore,
the vegetation on each side is subjected to differing flushing characteris-
tics.

     Although the oil penetrated almost everywhere in the back marsh, a few
regions were protected.   Station 9, west of the Turnpike, was protected by
the Turnpike on one side, by a gas pipeline on the opposite side, and by
embankments and substantial expanses of marsh grass on either end.   This
grass apparently served to trap most of the oil before it reached the sample
station, for no signs of contamination were visible at the first sampling
period.  Station 7, east of the Turnpike, was similarly protected by a berm
and extensive marsh.

     The two other vegetation sampling stations, numbers 8 and 10,  were
located adjacent to channels on the east and west side, respectively, of the
New Jersey Turnpike.  Both had been oiled, but the contamination was no
longer extensive by the time the research team arrived.  Apparently, most
locations in the back marsh had been exposed to a substantial amount of
back-and-forth tidal flushing that helped clean the marsh grasses.

     Two additional stations were established on the mudflats to observe
penetration of the spilled oil into the sediments and to sample the inver-
tebrate fauna for possible response to the spill.   Station 6 was located in
the east and Station 11 on the west side of the New Jersey Turnpike.

     Table I summarizes  the characteristics of the sampling stations.

                    TABLE 1.   SUMMARY OF SAMPLE STATIONS


Treatments
Oiled
Cut
Type
Location
Key: P =
C -•"•
M =
R =
E =
W =



1234
0 0
0 0
P S&P P P
R R R R
Stations
5 6 7 8 9 10 11
00 0 00
S M S S S S M
R E E E W W W
Phragmites marsh
Spartina marsh
Mudflats
River bank
Back marsh
Back marsh

east of Turnpike
west of Turnpike


                                     18

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SAMPLING PROCEDURE

     Data were collected during four sampling sessions:   in July 1976;
October 1976; March 1977; and July 1977.   The following paragraphs  describe
the data-collection procedures followed at each sampling period.

Vegetational Distribution and Productivity

     During the initial sampling period,  a transect was established parallel
to the adjacent river or tributary stream channel  at each of the 9  sample
stations that supported vegetation.   The position  of the initial point  of  the
transect in relation to the station marker and the transect's direction were
recorded.  Each transect was subjectively positioned so that all points along
its length would be equally exposed to tides.  Samples were taken in five
1-meter-square quadrats at 3.05-meter (10-foot) intervals along the transect.
For a few of,these sample plots, this nominal interval was adjusted to  avoid
significant irregularities in bank conformation like drainage sloughs and
similar features.   The sampling arrangement is depicted in Figure 7.

     The location of each quadrat was recorded so  that the quadrats could  be
re-established by use of measuring tape and pocket transit at subsequent
sampling periods.   After the winter, when investigators were confident that
markers would remain stable, the boundaries of each quadrat were delineated
with stakes and twine.  This considerably expedited and improved the accuracy
of quadrat re-establishment in the final sampling period.

     The complexity of the terrain and the consequent difficulty of estab-
lishing similar sampling sites dictated the use of nondestructive sampling
techniques.  The investigators measured percent cover, stem density, stem
height, and stem diameter of grasses at each sample station.  Where the marsh
grasses were short enough, the percent of ground surface area covered by each
species was determined with a sampling frame.  Elsewhere,  it was necessary to
estimate this quantity.  The estimation was simplified and probably improved
by placing 2 meter sticks so that each quadrat was divided into four 1/4-
square-meter subplots.  Each of these smaller units could  be visualized more
easily than the entire quadrat.  Percentages of cover for  each  quarter-
quadrat were estimated, summed, and divided by 4 to obtain the  final estimate
for the entire quadrat.

     Stem densities were determined by counting the number of stems in 2 of
the 1/4-square-meter subunits of each quadrat, resulting in 10  measurements
per station per sample period.  All shoots arising independently from the
soil were counted as separate stems, whether or not they were attached to the
same parent plant or not.  Some problems are associated with this method of
single-plant identification, as Caldwell (1957) has discussed.  Nonetheless,
the rest of the sampling procedure was designed around it.  The ultimate goal
was to obtain various  indicators and measures of plant productivity within
the quadrats, not to measure the size of individual plants, so  this approach
appears entirely justified.
                                      19

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                       DStation Marker
                                                         to Station
                                                         Marker
                      Plot
                               1 meter
          River
3.05 meters
River
                                              Density
                                                           Density
                 Expanded View of
                 Individual Plot

            Stem counts were recorded for
       indicated i-m2 subplots.
            In plots 1, 3, and 5, stem
       height and diameter were recorded
       for ten plants.  Plants measured
       were those lying nearest to points
       at 0.1-meter intervals along the
       transverse axis of the plot, on
       the far side of the axis from the
       station marker.
            This nominal  configuration
       was altered at some stations to
       conform to local constraints 1n
       topography.
   Figure 7.  Configuration of Vegetational  Sampling  Stations
                                20

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     In the first, third,  and fifth quadrats at each station,  the  height  and
diameter of individual  grass steins were recorded for 10 plants.  The  plants
selected were those lying  nearest to points at 0.1-meter intervals along  the
transverse axis of the  sample plot, on the far side of the axis  from  the
station marker.  Occasionally, there were not enough stems in  appropriate
locations; in such cases,  other stems in or very near the quadrat, along  the
transect, were measured.   Height was measured with a meter stick,  to  the  tip
of the longest leaves in Spartina, and to the tip of the most  recently
developed, unopened leaf at the apex of Phragmites stems.  Stem  diameter  was
measured with a micrometer-caliper.

     The simplest assumption for calculating the volumes of the  plants was to
treat them as if they were cone-shaped, and to use the following formula:

     Stem volume = ir x (Height) x (Diameter)2
                         12

By taking the average volume of the stems in a given quadrat and multiplying
it by the number of stems  in that quadrat, a figure was obtained that esti-
mated the total volume of vegetation in the plot.  If it is assumed that
volume of the plants is proportional to dry weight, this figure  is an indi-
cator of the biological productivity (fixed biomass) of the plot.   Despite
inaccuracies that may arise from some of the above assumptions,  this estimate
was useful because it permitted meaningful comparison of any 2 sample plots,
whatever their dominant species.  This is not true of stem height, diameter,
or density, which are likely to differ between species.  Although estimations
of percent cover permit some comparison of the amount of light used by plants
in the different quadrats, they fail to incorporate potentially significant
differences in plant height.

     In addition to the measurements described above, false-color infrared
and true color aerial photographs were taken at each sample period.  This
program was intended to document any extensive plant mortalities  that might
have resulted from the spill.

Fauna

     At each station, 5 samples of marsh soil were collected by hand, using a
small, metal container.  Care was taken to sample the same surface area  at
each location, but the depth of marsh soil collected varied where roots  and
rhizomes interfered with sampling.  The samples were washed free  of sediment
in a 2-millimeter wire mesh and preserved in 10 percent  buffered  formalin
solution.  Selected samples were shipped to a biological  laboratory after
each sampling period for identification of all invertebrate fauna.

     Interviews with knowledgeable persons furnished some information on the
apparent response of birds and other prominent marshland  species  to the  oil
spill.
                                     21

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 Distribution of Oil  in the Substrate

      Duplicate marsh soil  cores were collected at each sample station,  with  a
 stainless steel  corer.   The core samples were placed in airtight plastic
 bags,  wrapped in aluminum  foil, and frozen in dry ice immediately after
 collection.

      Selected samples,  from 2.5,  5.0,  and 7.5 centimeters  depth  in the  marsh
 soil,  were sent  to a laboratory for chemical  analysis.   It was originally
 hoped  that gas chromotography  (GC)  could be used to  identify  the oil  and
 estimate its concentration in  the marsh  substrate.   Unfortunately,  GC
 analyses that were performed after  the first sampling period  produced no
 clear-cut results.   Thin-layer chromatography (TLC)  was subsequently  used  as
 the  analytic technique.  TLC is less sensitive than  GC, but it permits  rapid
 screening of a large number of samples without entailing excessive  costs.  No
 true reference material  (Wellen No.  6) was  available when  the current investi-
 gation was initiated.   It  was  hoped that internal  comparisons of the  sample
 stations would indicate  the presence or  absence  of oil  at  any given station.
 Comparison was also  made to a  sample of  oiled gravel.   This sample was  free
 of organic sediments  that  would appear as peaks  in the  chromatogram and mask
 the  oil's presence.

 Erosion

     Erosional data were collected  at  all vegetated  sample  stations.  Two
 wooden stakes, one closer  to the marsh interior  and  one nearer the adjacent
 water  body,  were marked, notched, and  placed  in  the  soil at measured  dis-
 tances and directions from  the  station marker.   At each sample period, the
 height of the  notch on each  stake was  measured.  These  data provided  a con-
 tinuing  record of the elevation of  the marsh  soil  surface.   This permitted
 monitoring for sediment  loss that might  accompany the death of marsh  vegeta-
 tion in  oiled  areas and  the  heavy foot traffic in cut regions.

     Distances were also measured from the marker stakes to the major break
 in slope  at  the erosional face of the  river bank at Stations  1 through 5.

     The  aerial photographs taken as a portion of the vegetational sampling
program were also intended to extend the observations of erosion processes
beyond the individual stations  where measurements were made.  Additional,
qualitative data were gathered  on other factors that could potentially affect
erosion.   These included tidal  flow, boat traffic, bank conformation,  and
general substrate characteristics.
                                     22

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                                  SECTION 5

                                   RESULTS
     All  results are presented in figures or tables in the Appendix.  For
convenience to the reader, selected data are also presented in the text.
VEGETATION
     The data on vegetational characteristics of each sample station are
displayed in Figures A-l through A-6 and in Tables A-l and A-2.

     Figure 8, "Total Plant Volume per Unit Area" shows the pattern of
standing vegetational crop throughout the year of study.  The initially large
amount of plant material present in July 1976 decreased in October because of
dieback and decay.  This effect is more pronounced in Spartina. whose stems
are softer, more pliable, and more susceptible to the elements, than are
Phragmites stems.  The data collection in March did not include dead stems
from the previous year's growth.  The volume of vegetation in that month
represents the small shoots of the new growing season.  In July 1977, the
amount of plant material present is similar to what it was the previous year.

•  iolce d.a,JVu?ges* that there 1s less Phragmites at Station 1 in 1977 than
in iy/b.   While it might at first be surmised that this was caused by oil
contamination, this is probably not the case.   At the final sampling period,
the charred remains of marker stakes and other debris provided convincing
evidence that a fire had destroyed the surface vegetation at the station   As
discussed in Section 2, fire breaks the dormancy of Phragmites buds.  The
stems measured in July 1977 had resprouted, but had not as many dormant buds
nor the same time to grow as the previous year's stems.

     The control  Phragmites at Station 3 seem to have grown back quite vigor-
ously in 1977.   Interestingly, the plants at Station 4,  which were experi-
mentally cut, recovered only as much as those at Station 1, even though
Station 4 appeared similar to Station 3 at the study's outset.

     The Spartina controls (Stations 7 and 9)  grew back extraordinarily well
by July 1977.   The apparently spectacular increase at Station 9 might, how-
ever  have resulted from the station marker's  being lost over the winter
The transect may  have been re-established in a slightly different location
resulting in misleading data.   Nonetheless, the marsh surrounding Station 9

                                                     ™ ppobably
                                     23

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-4500




-4000




-3500




-3000




-2500



-2000




-1500




-1000




-500
M
/> v
                                                                     1
                                                                     !,
                                                                   It
 Station
 Oiled
 Cut
 Pom.Species
 Location
 Time
                 10
Phrag    Spartina
  River
     July '76
                  1
 Phrag |   Spartina
   River
      Oct.  '76
Phrag
               9 10
Spartina
  River
      Mar.  '77
                                                                                i

                               10
Phrag|   Spartina
                River
                                                                                    w
                                                                                      July '77
                       Figure 8.   Total Plant Volume  Per Unit Area  (cm /m )

-------
      The  data  suggest that the uncut, oiled Spartina to the west of the New
 Jersey Turnpike  (Station 10) did not grow weTT  Tfie decrease may reflect  a
 loss  of substrate  rather than poor plant growth, since it appeared that there
 was  less  available substrate for Spartina after the winter of 1976-1977 and
 since Spartina grew very well at Station 10 wherever there was available
 substrate.  This observation is supported by 2 groups of data.   First  the
 percent cover  (see Figure A-l) was not much lower than at other stations.
 [he tall  grass stems there leaned over and covered unvegetated portions of
 the sample plots.   Second, the stem density (Figure A-2) compares favorably
 with  that at other stations in July 1977, despite the paucity of substrate at
 station 10.
 T     ^e  ^uh 01l!d SPart1na 1n the back marsh east of the New Jersey
 Turnpike  (Station 8) exhibited good growth by the final  sampling period,
 although  not as good as the control Spartina.   The soil  at Station  8 was very
 moist.  The ground contained many poorly drained depressions.

       e  °lledl cu!;. Plants at Station 2 grew to approximately the same total
                       at Station 8 in July 1977.   They  did not produce as
mnrh    H'H  .                                   .
SSl,X-    i^K000^!1 Phragmites at Station 3> but exceeded the  concurrent
production of Phragmites at Stations 1 and 4.
            h          /"easurements at Station 5 were taken on  the young
          LJf? f f  M  ° repUce the contaminated Spartina stems when this
    nn«h 2  I JS ; *"° mea*urements of the oiled mass of dead vegetation
    f?n!  1 cJ VhaV1me'  However, the second-year productivity of  the
    tina at Station 5 was unquestionably lower.       K          y
nprrrn    ™ste* **!*£> stem dens1ty,  individual  stem volume,  and
percent cover all support the data on total  plant volume.   Station 5 con-
                       Pr°dUCtive than the othe'"s-   ^ Diameter does not
        H fi6 d?"S1'ty °f ,Phragniites stems is  roughly the  same  at the
     e    t ?   samPlln9s:  The experimental  cut (Station 4) appears to have
     o?r SntleS? d%T  ^^ially-  -PerhaPs  some  stubble was  Campled into
size Sid'tpXESrS ?°      I9her denSlty °f Spartina'  ^fleeting  its smaller
size and tendency to sprout year-round.

     A few additional  observations  are  relevant to the  response  of  the marsh
land vegetation.   The  earliest cut  stands  of  oiled Spartina displayed aSod
growback of vegetation by July 1977.  Station 2 was-^nlTsiifh reg on   ff

Sfter tte sUSi]ierP?Mh;?nH°f ihe CUt  ^*>  Wh1ch was'cut  more  ^™ two weeks
ifto%t5iS J H-  i    5 d a 16SS P°sltlve  response.  Oiled, uncut regions
like Station 5 displayed poor growth  in 1977.

                  plan! m°rtall>ty wa* observed in  any aerial photographs.
                  UnC      3    °n the r1verbank' none was  observed from the
                                     25

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     Weathered oil, presumably from other spill  events,  was observed in some
locations, but not at any sampling stations.   At the final  sampling period,
oil from a small, recent spill was observed at Station 5 and along the west
bank of the Hackensack River north of Station 5.

FAUNA

     Donald Smith (personal communication) has reported that a number of bird
species that usually nest in the Hackensack Meadowlands were rare or absent
in 1976 after the marshes were contaminated by spilled oil.  However, by the
following year, populations were apparently back to normal  and thriving.
Several pairs of glossy ibis (Plegadis falcinellus), a species never observed
nesting in the Hackensack Meadowlands before, were observed and strongly
suspected of breeding there in the Spring of 1977.

     Smith also noted that, after the spill,  marsh and red-jointed fiddler
crabs (Uca pugnax, U. minax) and diamondback terrapin (Malaclemys terrapin)
were physically handicapped by contamination with tarry residues from the
oil.  Nonetheless, these creatures continued to attempt movement.  They did
not appear to be poisoned, as they might have upon exposure to a truly toxic
oil.  One other unusual effect was the appearance of mating coloration and
display in male fiddler crabs in October 1976, well past the usual mating
season (Donald Smith, personal communication).

     The data on marsh soil invertebrates are presented in  Tables A-3 and
A-4.  Data include the habitat conditions, populations of various taxa,
number of genera, number of individuals, and Shannon-Weaver diversity index
for each sampled station.   The population levels in 1977 were generally lower
than those in 1976.  The populations of oligochaete worms were observed to be
higher than that of other taxa in most samples.   In the first and second
samplings, the lowest species diversity was found at those  stations where the
vegetation had most recently been cut in 1976, i.e., Station 2 in July, and
Station 4 in October.  Calculated diversity indices, dependent to a degree
upon population size, were lower in the final two samplings.

     No organisms were observed at the oiled mudflat station, number 6 during
the first July sampling.   It could be argued that this was  an effect of the
oil.  However, since other oiled stations (e.g., 1 and 2) displayed sub-
stantial populations, it was felt that some other effect (perhaps toxic
runoff from the New Jersey Turnpike) was being observed.  The mudflat
stations were subsequently abandoned and Stations 7 and 8 were substituted in
subsequent sampling sessions.

OIL IN THE SUBSTRATE

     Internal comparison of the thin-layer chromatograms derived from soil
cores at selected sample stations revealed several points of similarity among
the organic compounds in the substrate at each station.   However, these were
apparently of biogenic origin, because they bore no similarity to the oil in
the gravel sample which served as a reference in this analysis.  Possible
interpretations of these negative results are discussed in  Section 6.
                                      26

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 EROSION
      Observed  interim  and  net  changes  in  elevation of the marsh surface are
 shown in Table A-5.  A net loss  of  soil is  indicated by most river bank data
 and a net gain is  suggested by data from  the marsh interior.  These observed
 changes  may be due in  part to  sinking  of  marker  stakes or isostatic adjust-
 ments in the marsh surface.

      Apparent  sediment accretion is most  widely  observed between the first
 and second samplings.   The predominantly  negative changes between the second
 and third sample periods may reflect marsh  sediment compaction associated
 with heavy winter  ice  loading.   The final data suggest little change.

      Table A-6 displays distances to the  erosional face in river bank areas.
 These are presented graphically  in  Figure 9.  Note that the intervals between
 sample periods are not equal.  No substantial difference is suggested by the
 data for uncut oiled  area (Station 1) or uncut  and experimentally cut,
 unoiled  areas  (Stations 3  and  4).   In  contrast,  a radically different rate of
 erosion  between the first  and  second sampling occurred at Station 2.  This
 station  was  exposed to both  oil  and heavy manual cutting operations.  Aerial
 photographs  and ground observation  confirmed that this extreme bank erosion
 had occurred along much of the riverbank  where the oiled plants had been cut.
 Furthermore, the severe erosion  was  restricted to the cut regions.

      The mechanism of  erosion  in this  case was bank undercutting followed by
 slumping  Many private and  commercial boats and ships were observed on the
 Hackensack River during all  sampling periods.  The wakes produced by this
 traffic  were sufficient to  have  provided  the primary erosional force.  The
 surficial  mat  of Spartina  roots  remained  essentially intact and continued to
 support  growing plants throughout the  investigation.   The riverbank at
 and wavp  r!i?S       ^ at  Stations 1, 3, and 4, and consisted of a current-

 32 cTzone SrnW-stl1        " ^ ^ "^ <™ »«* °f
net 1        o> Sl!^^^
     Examination of aerial photographs and ground observation yielded the
nll^n10nf +K  e-OST ?f a different type had occurred along the later-cut
portions of the riverbank.  In these locations, soil appeared to have been

w?nter o?         *" °f ^ banL   Thl'S condiilon ^noticed after the
                                      27

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- 0
--50
--100
    Distance From
   Initial  Location
         (cm)
                                        Station 5
                                        Station 4
                                        Station 1
                                                              Station 3
                                            •	(lost marker) Station 2
 -150
                                      Time
    July  '76
Oct. '76
Mar. '77
July '77
                      Figure  9.   Position of Bank Edge Vs. Time
                                      28

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                                  SECTION 6

                                 DISCUSSION


     All oil spills are different in some respects.  The findings reported in
the present study are applicable only to spills where a similar type of
relatively viscous, heavy fraction oil is spilled in a similar estuarine
environment.  However, since such oils are frequently spilled, the results
presented herein are likely to have wide applicability.

     Patterns of tidal flow that distribute spilled oil also partly determine
the ecological characteristics of the affected region.  Thus, areas isolated
from contamination may not be directly comparable to oiled regions.  For
instance, the vigorous growth of Spartina at Stations 7 and 9 in the final
sampling may result more from their locations away from open water than from
the fact that they weren't oiled.  The present study affords no purely ob-
jective way of distinguishing among these possibilities.  The author's own
impression is that the difference is locational.

     Because the sample sizes were rather small and the data quite variable,
the quantitative results reported here should be considered indicative and
supportive only of observations made in text, not as conclusive evidence.•
Nonetheless, considerable use can and should be made of these data in the
design of future studies, because they provide estimates of the variability
of the sampled material.   These estimates are extremely useful in determining
the optimal sample size,  sampling design and the probability of obtaining
meaningful results within given cost constraints.

     Finally, it should be emphasized that no detailed quantitative baseline
data were available for this study.   A similar difficulty is likely to plague
many future attempts at determining the effects of any large-scale impact
upon an ecosystem.

SOURCES OF ERROR

     The most prominent sources of error in the present study have been
uncontrolled variables.   Sampling sites were selected to be as similar as
possible, but it was apparent from the start that the stations were not
precisely equivalent.

     The vegetation data are extremely variable.  The standard deviations
(displayed as dark lines  superimposed on the histogram bars in Figures A-l
through A-6) are generally large, compared to the mean value (length of the
bar) of. any given measurement.   This variability could be a result of the
small  number of samples  used in this study;  more likely it results from the
                                      29

-------
 clumping  of  data.   Clumping  simply means  that observed characteristics are
 not  independent.   For  example,  if a  sample contains one large stem, there is
 a  greater-than-random  chance  that the  next measured stem will be large.  Data
 for  such  clumped  distributions  characteristically have a large standard
 deviation.

      In the  case  of data on  the percent cover and density, the clumping was
 caused by the physical clustering of Spartina and Phragmites plants.
 Spartina  forms clones:  small, young shoots arise from rhizomes (horizontal,
 underground  stems)  that sprout  from a  large parent stem.  Caldwell  (1957) has
 described this process in detail.  To  a lesser extent, Phragmites does the
 same.  However, Phragmites is more evenly spaced, as evidenced by the smaller
 standard  deviations in the Phragmites  density (see Figure A-2).*  Clumping
 also  reflects the  tendency of these and many other plant species to cluster
 in optimal microhabitats.

      The  clumped  distribution of stem  height and diameter results less
 directly  from the  physical clustering  of the plants.   Stems selected to be
 measured  were those lying closest to certain points on a small transect that
 bisected  each quadrat.  If this transect failed to cut through clumps, a
 disproportionate  number of small stems on the clump edges were measured.   If
 the transect did pass through clumps,  more large stems were measured.

      The  high variability in height and diameter is reflected in an even
 greater standard deviation of individual stem volume (Figure A-6).   Limited
 resources and the  rather lengthy calculations required prevented the deter-
 mination  of the standard deviation of  total plant volume per square meter
 (Figure A-3).  Nevertheless, it follows from the variability of stem height
 and diameter that the standard deviation of stem volume would have been quite
 large.

      If it is assumed that the probability of selecting a cluster of large or
 small stems is approximately equal to  the true proportion of large and small
 stems in  the sample plot, then the calculated mean will be a much better
 estimate  of the true mean stem size than the large standard deviation would
 imply.  A similar argument holds true  for percent cover and stem density.

      The  above assumption failed only  in instances where the clumps were so
 sparse that a sample point was unlikely to fall  in the center of one.   In
 these cases, there probably was some bias towards sampling small  stems.  This
 bias would have accentuated measured differences between lushly and sparsely
 vegetated plots.   However, except for Stations 8 and 10 in July 1977,  sparse
plots also had very open clumps, reducing the effects of the bias.   The data
are therefore considered reliable, though interpreted cautiously.
*The very large standard deviation of Phragmites density at Station 1 in
 July 1976, is the result of using a 1-square-decimeter quadrat for counting
 densities.  On the basis of this sample, this quadrat size was judged to be
 too small, and 1/4-square-meter quadrats were subsequently adopted.
                                      30

-------
 VEGETATION
      Most of the oiled,  uncut  sample stations have been affected in some way

 besides oiling that clouds  the interpretation of data.  Station 1 was burned-

 affliJUn f3.nd Station 10 were  characterized by irregular substrate that
 affected the distribution of the plants.  It is possible that the low produc-

 ?™ *:e! °*!?™d ™ Ju]y I977 at.all these sites are due to oil.   Thefol-
 w,-      ev/1dence that at Stations 8 and 10 the differences
 were due at least in  part to habitat.


      Explanations for the loss of substrate at Station 10 were suggested  by  a

 morP 0b?h7Sl??S- • T?6 SHtl0!! WaS heavily ticked by fishermen9  Further-
 more,  the station s location between 2 embankments might have encouraged

 mnurIVA ^ln9 t* 1Ce;  T?e ^C°rded hei'9hts of P'ants at Station 10
 re^oncihi^f^th01!9^31'  I* 1S be11eved that the bias discussed above is

 rnntHh.fi tl ^    t J°V recorded stem ^^-   This would also ultimately
 contribute to low total  volume.  Station 8 contained many poorly-drained

 sCch rPninLmnJtSh*  As ^ f al '  (1975) have discussed, the Spartina in

 Some o?9tS2 cj£i  Tf in dePauPerate stands-   Station 8 was no  exception.
 Some of the sample plots were sparsely vegetated.   Yet, even where the veqe-

 S?ai?onWISwf?arSe> T? \nd1v1dua1  clumPS of grass were quite heaUhy  Tus,

 degree         P°S          Same type °f bias as Stat1°" 10> but  to a
   receecothi-i   P??ductivity at Station 5 was  probably  a

 f  ected  in lu thl n   ] t^"'^ !he P°0f resP°nse of the vegetation  is re-
 wlr«  nnt   ?  /   Parameters that were measured.   Clumps at this  station

 r?uL2 inWmlIvdefeJKped) a?d the taller stems in thei> interiors were  S-
 cluded in many of the samples.   Furthermore, the oiled remains of  marsh

 grasses  growing prior to the spill were sti 1 present in July  1976   These

                                          than those wh?ch  ^w9in'l977 ^

                                             °f heavily °11ed  and  matted
samolina suaaPJ?^h^ntar'-ati°n  (source  unknown) observed at the final
to the same ?o itt?™ «'   JS  freclu?nt1y carried by the extant current regime
that ? hf !  co'lecting points,  causing  chronic pollution.  It is possible
that the poor growth at Station 5  partly results from continua  oilina ov
                                                                      over
     moedamainnh                     '
much more damaging than a single  incident  (Baker, 1971b).


     Very little Phragmites was present at Station 2 in July 1977   Perhaos

s?le  P      neSedmaJ.COnent °f the veget^tton at this P
se    t    ancK.
PhraamitL in !h^P   lblG that CUtting  favored  the Spartina and damaged the
Phragmites in what was once more of a  mixed  stand, as discussed in Section 4.
tion  aon!;^ of,^lna was  observed at Station 2, where the vegeta-

illl   ?h«U ? a?  cu?'  /arther  uPriver the Olled, cut grass did not grow so
etl  H977^      15antKrrSCU^1aJerthantheother   however.  Mittson
et al.  (1977) concluded that  the  oil plugged the stomata of the Spartina
                                     31

-------
plants, thus depriving their roots of air (see Section 2).   The roots of the
earliest-cut plants were saved from suffocation when the cutting operations
opened the plant stems, allowing air to diffuse downward.   Approximately
2 weeks after the initial oiling, the remaining contaminated roots of Spartina
started to die for want of oxygen; thus, later cutting was less effective in
promoting recovery.

     The results of the present study support the conclusions of Mattson et
al. (1977).   In areas that were oiled but where the plants were not com-
pletely coated (e.g., Station 1) or where tidal flushing washed plants partly
clean (e.g., Stations 8 and 10), there is no conclusive evidence of oil
damage.  It therefore seems unlikely that the oil exerted its negative ef-
fects upon the marsh grasses through any toxic mechanism.   Cutting was appar-
ently an effective way of combatting the physical blockage by oil of the
plants' gaseous exchange with the atmosphere.  It was therefore desirable in
this case.

     Unfortunately, it cannot be generalized from this study that cutting is
a panacea for oil damage to marsh plants.  Other factors may be significant
in other situations.  Seasonal effects and the biology of the affected species
 must be considered.  For example, it is possible that the apparent decrease
in the standing crop of Phragmites at Station 4 was caused by the cutting
that was performed when this study began (Section 4).  Results might also
differ for oils with higher concentration of toxic components.  If toxic oils
were trampled into the soil during cutting, they might persist indefinitely
and preclude recolonization.  Cutting might encourage migration of toxic
materials down the stems to the roots and rhizomes of affected plants.

     Possible alternatives to hand-cutting include use of aquatic vegetation
cutters and burning.  Both techniques minimize foot traffic on potentially
sensitive marsh soil.  Although ignition during the growing season may be
difficult, burning has the added advantage of promoting sprouting in
Phragmites.   This technique would have to be used in the context of air
quality and safety standards, as well as vegetational management options,
since burning can alter the composition of marsh plant populations.  None-
theless, burning is probably the available technology most compatible with
natural functioning and continued maximal productivity of the marsh.

FAUNA

     The mating coloration and behavior of male fiddler crabs in October 1976
may have resulted from harmonal interference caused by petroleum hydrocarbons
still leaching from the marsh soil at that time.  This phenomenon, which was
not observed i/i 1977, has been observed elsewhere (Charles Krebs, Saint
Mary's College, personal communication).

     It does not appear that the large vertebrates of the Hackensack Meadow-
lands, especially the birds, were adversely affected for longer than the
season of the spill itself.
                                     32

-------
      There is no clear correlation  between any properties of the sampled
 invertebrate communities and the  presence or absence of oiling.  The dense
 population of oligochaete worms  is  typical of a highly organic substrate like
 that found in the Meadowlands.  The lowered diversity index of fauna at cut
 stations may be an indication either of the direct effects of cutting and
 trampling on the marsh soil  fauna or of the reduction in habitat diversity
 that accompanied the loss of the  grasses.  The lowered index may also have
 resulted from some other locational  effect, or it may simply be an artifact
 of the small sample size.  The interpretations offered here must therefore be
 considered t.pntatiuo
      The lack of observed  organisms at Station 3 in the third and fourth
 sample periods and  the  low diversity  indices in 1977 probably reflect the
    cra    -°W numbers °f organisms present at that time.  The harsh winter of
     ~
         7 -                                            .
      nn.Ll     Possible  cause of the population reduction.  It is not likely
      "?r!covered  Ol1  "used the decrease, since the oil spilled in the Hacken-
 sack River was  apparently physically disabling but not toxic.   Moreover, the
 population reduction  is  not related to the known distribution of the oil.
 Hn,tthoi       unfortunate that benthic monitoring was not con-
 tinued  at  the mudflat Stations 6 and 11 after the first sample period.
 *nH  th2 J'       supported a variety of organisms, was only lightly oiled
 and  therefore served as a control for the more heavily oiled Station 6   Even
  f t*ic or otn    ffcts unrelat
 .                         unrelated  to  oil  contamination were present at
btat on 6,  the site might normally have supported some  invertebrates.  The
complete absence of organisms  in  samples collected at this station  however
                        Oil1n9 ^ have ^ed the  soil fauna.  A subsequent
  n™--                                                 .
 iunnnrt nintMVertel?raJes atustat1on 6 "ould have provided evidence in
 support of this conjecture, but no additional data were collected   Neverthe-
 less  samples collected at this location at any time in the futSre would be

    a
OIL IN SUBSTRATE
     No correlation was established between the organic materials in the soil
tLTJr±red at Van'°US Sam?ling Stat1ons and th* samPle of o  ed grave
thf n^v ?d    ? referen«-   There is no question that the bulk of the  oil  in
2c,,?E  • hfT16 Came from-the We11en sP1n-   Therefore,  although posit ve
results m ght have been subject to dispute in  the absence  of a true reference
JKtn2attVh reSKltS PSrted here must be Considered as reliable Llhose'
that would have been obtained from an uncontaminated sample of the spilled
            he Seve^al DOS?1b1e explanations  for the  observed  lack of cor-
   +           samPled sediments may simply not have  been oiled to a level
that would have been detectable by TLC.   Furthermore,  highly viscous oils are
unlikely to penetrate soil,  especially a marsh  soil composed of very fine
sediment.   In fact,  the contaminating oil  in  this  spill  formed only a thin,
                                     33

-------
tarry  layer on the marsh soil surface at the locations where soil contamina-
tion was still apparent as of mid-July 1976.  There was no indication of soil
penetration.

     It is interesting to note that the results indicate no soil contamina-
tion at Station 2, which was subjected to heavy foot traffic shortly after
the spill.  Perhaps the soil there was firm enough to prevent mixing of oil
with any but the most unconsolidated upper layers of sediment.  The surface
sediment that was disturbed and heavily contaminated may have washed away
during subsequent high tides.

     A final possibility is that the oil which contaminated the gravel sample
weathered differently from that which contaminated the marsh surface.  However,
it is unlikely that the decomposition processes could have been so different
as to completely mask any similarity within 2 months after the spill, when
the soil cores were first collected.

     Further speculation is not warranted in the absence of more definitive
data.  Future studies should probably continue to use TLC as a screening
procedure before more sophisticated and costly forms of analysis are used.
However, such tests are more likely to be more successful if samples are
containing a high concentration of the suspected contaminating oil are
collected.  For example, in this investigation, the upper few millimeters
instead of the upper few centimeters of oiled marsh soil would have been
collected and then compared with similar samples at greater depths in the
soil.

EROSION

     It is difficult to ignore the association between cutting and acceler-
ated bank erosion at Station 2.   Because the Spartina root mat remained
undamaged, the erosion cannot be attributed to plant mortality.  The pressure
of heavy foot traffic may have been transmitted through the root mat to the
unconsolidated soil below, resulting in destabilization.  Although they may
have been contributing factors,  neither oiling nor exposure to the open river
channel can be implicated as sole causes of erosion, since Station 1 was also
both oiled and situated on the main river bank but it did not erode as much
as Station 2.

 t   The shape of the bank may have some effect on erosion potential.  The
northern portion of the cut region, where the bank slope was less steep than
at Station 2,  did not experience bank undercutting.   However, the oiled
plants on this portion of the bank were cut last.   It is possible that foot
traffic associated with cutting operations was less intensive toward the end
of the cleanup effort.

     The Phragmites at Station 4 was cut so that the effects of cutting in
the absence of oil contamination could be observed.   The lack of unoiled
river frontage necessitated locating this station on an interior channel.
The absence of erosion at this site may be attributed either to lack of
                                      34

-------
oiling or to the low wave and current energy environment.   The latter ex-
planation appears more likely, since high-energy environments are known to
cause erosion.   It is also possible that the deeper root mat of Phragmites
plants provides more bank stabilization than that of Spartina.   This would be
consistent with all observations.                       	

     The positive values observed at Station 5 probably reflect the sedi-
mentological environment.  This station is on the inside of a bend in the
river, a natural point of accumulation.

     The subjectively observed surface erosion in the later-cut regions was
probably caused by winter icing and spring runoff through bank areas where
the plant cover was no longer intact.   There is no way to predict with cer-
tainty whether and to what degree this erosion might have been reduced had
the plant cover been maintained at its original vigor.
                                     35

-------
                                  REFERENCES
 Baker, Jenifer M.,  1971a.   The  effects  of  oils  on  plant  physiology.   In
      Effects  of Oil  Pollution on  Littoral  Communities, Institute  of
      Petroleum, London.

 Baker, Jenifer M.,  1971b.   Successive spillages.   In  E.B.  Cowell,  ed., The
      Ecological Effects  of  Oil  Pollution on  Littoral  Communities,  Institute
      of  Petroleum,  London.

 Burns, Katherine  A.  and  John M. Teal, 1971.  Hydrocarbon Incorporation into
      the Salt Marsh  Ecosystem from  the  West  Falmouth  Oil  Spill, NTIS  report.
      com 73 10419.

 Buttery,  B.R., W.T.  Williams, and J.M.  Lambert, 1965.  Competition between
      Glyceria maxima and Phragmites communis in the region of Surlingham
      Broad.ITTfie fen gradient.  Journal of Ecology  -  53:  183-196.

 Caldwell, Phoebe  - Ann,  1957.   The  spatial development of  Spartina colonies
      growing  without competition, Annals of  Botany, N.S. 21(82):203-214.

 Crapp, Geoffrey B.,  1971.   The  ecological  effects  of  stranded oil.  In E.B.
      Cowell,  ed., The Ecological Effects of Oil Pollution  on Littoral
      Communities. Institute of  Petroleum,  London.

 Environmental Protection Agency, 1976.  RFP No. CI-76-0322, Edison,
      New Jersey.

 Hackensack Meadowlands Development Commission, 1975.  Wetland Bio-Zones of
      the  Hackensack  Meadowlands:  An Inventory.  Lyndhurst, New Jersey.

 Haslam,  Sylvia M., 1970.  The performance  of Phragmites  communis Trin. in
      relation to water-supply.   Annals  of  Botany 34:567-877.

 Haslam, Sylvia M., 1968.  The development  and emergence  of buds in Phragmites
      communis Trin.   Annals of Botany 33:   289-301.

Haslam, Sylvia M., 1971.  Community regulation in  Phragmites communis Trin.
      II.  Mixed stands.   Journal  of Ecology 59(1):  75-88.

Haslam, Sylvia M., 1972.  Biological flora of the  British  Isles.  Phragmites
     communis Trin.   Journal of Ecology 60:  585-610.

Hubbard,  J.C.E.,  1970.   Effects  of cutting and seed production in Spartina
     anglica.   Journal  of Ecology 58(2):   329-334.
                                      36

-------
Mattson, Chester P., and others, 1977.  Hackensack estuary oil spill:
     cutting oil-soaked marsh grass as an innovative damage control
     technique.  In Proceedings, 1977 Oil Spill Conference.  American
     Petroleum Institute, Environmental Protection Agency, and United
     States Coast Guard, New Orleans.  243-246.

Mattson, Chester P., and Nicholas C. Vallario, 1976.  Water Quality in a
     Recovering Ecosystem, Hackensack Meadowlands Development Commission,
     Lyndhurst.

Patrick, Ruth, 1950.  Biological measure of stream conditions.  Sewage and
     Industrial Wastes 22(7):  926-938.	

Potera, George T., 1970.  A Preliminary Ecoloqic Study and Evaluation of
     a Section of the Hackensack Meadowlands.  Report to the Hackensack
     Meadowlands Development Commission.Lyndhurst, New Jersey.

Shea, M.L., R.S. Warren, and W.A. Nearing, 1975.  Biochemical and transplan-
     tation studies of the growth form of Spartina alterm'flora on Connecticut
     salt marshes.   Ecology 56:   461-466.

Teal, John, and Mildred Teal, 1969.  Life and Death of the Salt Marsh,
     National Audubon Society and Ballentine Books, New York.	

U.S.G.S., 1967a.  Jersey City Quadrangle 1:24000 topographic map.

U.S.G.S., 1967b.  Weehawken Quadrangle 1:24000 topographic map.

Waisel, Yoav., 1972.  Biology of Halophytes.  Academic Press, New York &
     London.                             	
                                      37

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CO
oo
                                                                              Table A-l


                                                                     Measurements  of  Phragmltes
Parameter Sample
Measured Period

1 Cover



Density .
(Steir,s/nr)


Height
(to,)


Diameter
(Cm)


Stem Volume
(Cm3)

Total Volume
(Density x
Ster, Volume)
(Cn.3)
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
Station No. 1
Standard
Mean Deviation
82
58
<.6
54
146
58.5
6.8
95.2
183'
181.7
2.75
103.3
.51
.674
.461
.486
18
24.4
.223
10.9
2,570
1.427
1.5
1.041
17.9
12.3
<.55
36
242
30.3
8.7
80.8
54.9
44.0
1.76
52.6
.24
.150
.185
.271
19
14.2
.270
15.4
__
..
--

Ho. of
Samples
5
4
5
5
15
8
10
10
36
25
15
28
36
25
15
28
36
25
15
28
__
..
--
Station No. 3
Standard
Mean Deviation
100
84
<)
96
120
49.2
31.2
107.6
205.9
234.8
6.4U
198.2
.50
.759
.810
.792
17
39.7
1.3
37.5
2,040
1.953
40.6
4,035
0
15.2
—
6.5
51.9
27.2
12.5
48.1
36.86
43.58
1.84
38.3
.18
.180
.0384
.208
18
26.1
.941
24.5
—
—
--
Station No. 4
No. of
Samples Mean
5
5
5
5
10
10
10
10
28
29
30
29
28
29
30
29
28
29
30
29
—
--
--

30
<1
56
70.4
43.6
23.2
85.6
„
67.3
10.86
127.2
.73
.442
.665
.594
__
4.41
1.63
15.5
—
192
37.8
1.327
Standard
Deviation

39
—
30
26.5
55.1
16.4
51.8
„
32.6
5.37
66.1
.24
.174
.266
.199
..
4.10
1.42
17.2
--
--
-
*
No. of
Samples

5
5
5
10
10
10
10
„
28
20
30
29
28
24
30
__
28
20
30
--
--
--
Station No. 2
Mean

1.8
..
5.2
__
0
11.2
18.8
__
5.2
4.48
86.1
._
.436
.422
.394
..
.235
.239
3.49
--
0
2.7
65.6
Standard
Deviation

2.9
--
5.6
__
0
11.4
19.8
_.
2.76
3.41
0
..
.0817
.0782
0
..
.0452
.295
0
--
--
--
llo. of
Samples

5
5
b
_.
10
10
10
__
2
4
1
_.
2
4
1
..
2
4
1
--
-•
--
       •Plot  4,  sample  period No. 2:
        only  within  plot plants.
the values for stem dimensions  include  plants not within sample plot.  These differed very little from values using
                                                                                                                                                                     -o
                                                                                                                                                                     -a
                                                                                                                                                                     ei
                                                                                                                                                                     i—i
                                                                                                                                                                     x

-------
                                                                  Table A-2


                                                           Measurements  of Spartina
to
to
Parameter
Measured

% Cover



Density _
(Stems/nT)


Height
(Cm)


Diameter
(Cm)


Stem Volume
(Cm*)

Total Volume
(Density x
Stem Volume)
(On3)
Sampl e
Period
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4

Mean
18
34
<1
86
76.
367
166
397
20.
11.
3.
55.


m

1.
t
m
5.
76.
76.
22.
2.064

Station No. 2
Standard
Deviation




7



8
2
92
7
35
202
120
500
0
207
0135
2
7
0
4

9.8
6.9
—
14.7
42.5
356
124
170
9.42
12.6
1.86
37.8
.19
.0879
.260
.235
1.4
.318
.0259
5.69
..
-~
__
~
No. of
Sampl e
5
5
5
5
15
10
10
10
30
28
26
29
30
28
26
29
30
28
26
29
_.
_..
_ _
—
Station No. 5
i Mean

2.2
<.6
20
18
27.6
34
146
10.3
15.3
3.62
23.9
.51
.285
.164
.427
1.2
.865
.0256
1.96
21.6
239
.9
286
Standard
Deviation

3.3
<.55
22
28.7
65.1
66.3
235
6.13
16.8
1.86
25
.26
.205
.0503
.213
1.8
1.69
.0150
2.73
„
__
	
«
Station No. 7
No. of
Samples Mean

5
5
5
8
10
10
10
19
30
10
20
19
30
10
20
19
30
10
20
„
__
_.

95
33
<1
94
221
349
299
554
94.1
19.8
5.46
52.8
.68
.228
.0531
.488
21
.661
.0351
7.67
4,641
231
6.9
4,249
Standard
Deviation
7.1
21.1
._
13
62.4
79
131
240
75.5
20.6
2.39
65.7
.30
.120
.0564
.251
25
1.47
.0311
12.3
..
__
__
—
No. of
Samples
5
5
5
5
10
10
10
10
30
29
29
30
30
29
29
30
30
29
29
30
„
__
__
—

-------
         Table A-2
Measurements of Spartina (Cont.)
Station No.
Parameter Sample
Measured Period

2 Cover



Density «
(Stems/m)

Height
(Cm)
\*** */

Diameter
(Cm)
\**' ' /

Stem Volume
(Cm3)
\ **' * i
Total Volume
(Density x
Stem Volume)
(Cm3)
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
1
2
3
4
Standard
Mean Deviation
64
13
<]
87
62.6
116
83.1
451
84.3
36.5
5.90
36.4
.65
.390
.224
.548
13
1.94
.0894
5.3
814
225
7.4
2,390
39.8
14.7
_.
20.0
54.6
93
113
295
67.4
33.7
2.53
53
.19
.206
.0780
.260
16
6.48
.0795
10.6
_—
—
--
—
, 8
Station No. 9

No. of Standard No. of
Samples Mean Deviation Samples
5
5
5
5
10
10
10
10
28
29
30
30
28
29
30
30
28
29
30
30
_„
—
—
—
74
45
<1
63
117
115
119
264
56.9
49.8
6.45
88.8
.53
.412
.212
.611
9.2
3.40
.107
15.8
1,075
391
12.7
4,171
31.5
26.7
—
24.8
70.8
84.7
184
134
41.75
32.06
2.63
65.1
.29
.169
.0917
.321
17
3.63
.117
18.4
__
—
—
--
5
5
5
5
10
10
10
10
29
30
30
30
29
30
30
30
29
30
30
30
—
—
--
~~
Station No. 10
Standard No. of
Mean Deviation Samples
82
55
<1
57
255
167
84
321
106.2
44.7
4.93
23.7
.62
.316
.154
.369
15
1.57
.0368
1.08
3,825
265
3.09
346
32.1
20.9
—
27.0
109
109
113
335
73.75
23.7
1.89
29.7
.21
.0953
.0559
.151
14
1.55
.0308
1.62
—
--
--
•~
5
5
5
5
8
8
10
10
20
20
30
30
20
20
30
30
20
20
30
30
—
--
--
•*••

-------
Phrag    Spartina
                        J
                        A A
                        y y
                        y y
                        y y
                        y y •
                        y y '
                        y y '
                        y y '

                        1  3 4  2 5  7  B 9
                   1C
Phrag    Spartina
                          River
                             UctT Vb
Phrag  Spartina
                   10
                         River      E    W

                             Mar.  '//
                         Figure f(-l.  %  Cover

-------
ro
-800
-700
-600
-500
-400
-300
-200
-100
Station
Oiled
Cut
Don. Species
Location
Time







|
-
i
t
>
i
i
j














i
i
i 4
\ »
I t
i •
; I
< »
\ 't
1
•















li
3


4

•
Phrag







I
2
•
•






-

I
5
9




/









t
8
•



9 1





g
•

Spartina
River
E
W
July '76





















j
1
•








i
3



"





I
4 i
t
• <
Phrag



*


I
! 5 '
» •
i












t 8
•



9 1





0
•

Spartina
River I E
w
Oct. '76





















x
1
•








i
3










4

•
Phrag



2
*
•





~~


I
5 ;
•













' 8
•




9 li
•

Spartina
River | E
W
Mar. '77


3
>











'
i
•'
i
1
i








1
•








j
3


t

t _

•*•


j
4251
• •
• •
Phrag Spa
River








'8910
• •

rtlna
E I W
July '77
                                      Figure A-2. Density (stems/m2)

-------
-4500
-4000
-3500
-3000
-2500 'f
-2000 | p
-« ||
-1000
II
-500
Station 134257
Oiled • • •
Cut • •





,
8
•







3



1C
•

Dom. Species Phrag Spartina
Location River E
Time July '76
W




i
* J,
•






1 	
3425789 10 1
• • • • •
• •
Phrag Spartina Ph

River E W
Oct. '76





	 \
3425789 10 JJ
• • • • •
• •




/




\ \
\ 1
1 1
| |
3


4

•
rag Spartina Phrag
River 1 E W
Mar. '77






2
•
•
I
5
•







/









8 <
•





\
10
•

Spartina
River

July
E
W
'77
                                                 3,  2,
Figure A-3.   Total  Plant Volume Per Unit Area  (cm /m )

-------
Phraq    Spartina
                        2
                     ' '  X
                     < I  ^
                          i
                     y  X ' '
                     c^  X ' '
                     ^  v! ' '
                     y  X ' '
                     
-------
-.9
-.8
-.7
-.6
" • 5 i
i
i
i
-.4 |
•
i
t
>
»


I
I
i
1
,


i
*
__ 4
1
J
j,
>
t
i
i
i
t
t
i
i
' i
\ \


•


> /j
t /*
' rj
1 /i
1 'J
> n
1 4V
' 'J
' ft
1 '',
•3 1 P P
I 1
I i i
"
' III
Station 1
Oiled •
Cut
3 4

•
Dom. Species Phrag



2
•
•






5 •
»
















1 8
•

-






9 1
«

Spartina
Location River | E
Time
W
July '76

't
i
»
t
t
t
t
t
t
\


>
t
'.
4

—


--
,
lu
11
1 1
\\ \
i i !
i i 1 1
in
0 1
» «

3


4

•
Phrag

2 5
• •
•

.

7 8
•


i >
i '
i i '
__ 1 1
1 1
1 1
* i
1 1
1 1
1

• •


i
\
%


*
t
t
t
1
|
|
|
3


»
'
t
i
7'


•
t
i
i
I
>
.
I
•
'
r
|
I
.
•
.
',
\
I
I
4

•
Spartina Phrag
River

E
W
Oct. '76






-
I






•

2
•
•

5
•





^
*
j
j
<
i
*
<
i
i
t
|
7 8
•

* •
r '<'
> i
1 1
1 1
1 1
1 1
> >
> '
t *
» >
4 '
/ i
* <
II
>$ '
Ifi


1
1
1
1
1
1
1
t
t
1
1
t
1
r
i y. '**
' % 2
1 Z y
1 X v
» 2 2
9 Z *
III
9 10 13
• •

4 1
t
• <
Spartina Phrag
River
Mar
E











2 5
» •
»






7 8
•

y







9 10
•

Spartloa
W River
E
W
. '77 Oct. '77
Figure A-5.Diameter (cm)

-------

-45
-35
-25


•
i


-15
i
.
l
i
i
i
-5
i
.
i
i
Station
Oiled
Cut
Don. Species
Location
Time





r
' ,

\\
!
•
I
i!
', '
]
•









r
.
•
•
,
-
|
|
'
'
•
'
"
•
.
.
•
3
















4

•
Phraq













I
2
•
•














5













T







I

[•
7


8
•











9


Spartina
River

E







!
/
X
/
1
f
/
I ;



!
i
^
>
r i
i
t
s
>
>
>
^
>
/
/
>
/
/
^
/
i
+
>
/
i
, ^
>
, ^
, i
, <
. ^
i ^
• f
, '
• f
i f
, J
i '
» J
. •
• \


i
r
|
f
'
,
,
1
,
i
,
t
,
,
t
,
.
i

,
r
,
i
|


r
.
\
; 2
1!
1 « ^
10
•

f
W
July '76
1
•

3















t
'
'
.-
*













j
t
/
/
/
4

•
3hrag
^iver














2
•
•














5
•















7






























8
•














i














9















i
10
•

Spartina

Oct.
E
W
'76


































1
•














I
3















^
4

•
Phrag














2
•
•














5
















7


Sparti
River
Mar.














8
•

na
E
77















9

















10
•


W


'

t
'
*
'
•'
t
*
*
*









•





'
*
1
t
•
'
'
1
1
1
1







•

.
i
i
i
i
i
i
i
i
i
»
i
i
i
i
•
i
i
i
t
i
i
i
»

i
M
1 !
5 ^ :
LI
1
•

3










.
,
,
•>
• ^_
p
1
I
.
1
|
f
t
•
f
t
>•
i
'
1
4

•
}hrag


?
•
•


-



_






l'
5
•







•
7






8
•












I
9 10
•

Spartina
^iver

E
W
July '77
Figure A-6.  Individual Stem Volume (on3)

-------
                                                                     Table A-3

                                                         HARSH SOIL INVERTEBRATE POPULATIONS

Habitat:
Sediment:
Flushing:*
Overflow
Throughput
Location:
Treatment:
Polychaetes

July '76


Oltgochaetes
Molluscs
Crustaceans
Insects
Polychaetes

Oct. '76

OKgochaetes
Molluscs
Crustaceans
Insects
Polychaetes

Mar. '77

OUgochaetes
Molluscs
Crustaceans
Insects
Polychoetes

Jul. '77

Oligochdctcs
Molluscs
Crustaceans
Insects
Station 1
Phragmites
Firm

Low
High
River
Oiled
64
258
0
3
0
0
65
0
6
0
4
38
0
0
0
0
n
\
0
0
Station 2
Spartlna marsh
Firm

Low
High
River
Oiled i Cut
6
533
0
1
0
0
3
0
1
0
0
75
0
0
0
0
90
0
0
0
Station 3
Phragmites
Firm

Lew
High
River
None
0
22
0
5
1
0
1
0
2
1
0
0
0
0
0
0
0
0
0
0
Station 4
Phragmites
Firm

Low
High
River
Cut
2
36
1
28
0
0
0
0
8
0
0
17
0
4
0
4
07
0
1
0
Station 6 Station 7
Mudflat Sparttna Harsh
Soft Soft

Low Low
Low Low
E E
Oiled None
0
0
0
0
0
0
31
0
32
1
3
41
0
3
0
9
no
47
10
0
Station 8 Station 11 Tide
Spartlna Harsh Mudflat
Soft Soft

Moderate Low
Low Low
E U
Oiled Oiled
809
29
1
0 1.2.3.4:Low
54 6.11:Higrt
227
448
0 Low
19
1
0
61
0 Low
2
0
49
6
1 Low
2
0
•Overflow « degree to which the plants themselves are flushed by tidal flows
 Throughput * rate at which surrounding waters are removed from the marsh system.

-------
00
                                                                                   Table  A-4

                                     HARSH SOIL FAUHA:   NUMBER OF INVERTEBRATE GENERA AIIO INDIVIDUALS.  AND INVERTEBRATE DIVERSITY  INDEX








July '76


Oct. '76


liar. '77

Jul. '77


Habitat:
Sedincnt:
Flushing:*
Overflow
Throughput
Location:
Treatment:
! Genera
Individuals
Diversity
Index**
/ Genera
1 Individuals
| Diversity
[ Index*"
1 Genera
Individuals
Diversity
Index**
(Genera
Individuals
(Diversity
Index"*
Station 1
Phragmites
Firm
Low
High
Rjver
Oiled
e
344
1.346
5
71
1.362
4
42
.800
4
73
.723
Station 2
Spartina marsh
Firm
Low
High
River
Oiled t Cut
11
542
.584
3
4
1.040
3
75
.555
2
100
.325
Station 3
Phragmites
Firm
Low
High
River
Hone
7
28
1.188
4
4
1.3B6
0
0
Undefined
0
0
Undefined
Station 4 Station 6 Station 7 Station 8 Station 11
Phragmites Mudflat Spartina Marsh Spartina Harsh lludflat
Firm Soft Soft Soft Soft
Low Low Low Moderate Low
High Low Low Low Low
River E E E »
Cut Oiled Hone Oiled Oiled
700 6
67 0 893
1.211 Undefined -443
2 6 9
8 67 692
.562 1-345 1.197
5 6 4
21 47 63
1.273 .838 .823
4 7 5
92 1U4 58
.503 1.219 .002
Tide







l,2.3,4:Low
6.11:High

Low


Low

Low

             •Overflow = degree to which the plants themselves are flushed by tidal  flows.
              Throughtput • rate at which surrounding waters are removed from the marsh system.
            '•Shannon-Weaver

-------
(£1
                                                                          Table  A-5
                                           Observed Changes in Soil  Surface  Elevation  at Sedimentation  Stakes  (cm)
Station
1
2
3
4
5
7
8
9
10
Description
Uncut oiled
river
Cut oiled
river
Uncut unoi led
tributary
Cut unoi led
tributary
Uncut oiled
river bend
Uncut unoi led
interior marsh
Uncut oi led
interior marsh
Uncut unoi led
interior marsh
Uncut oi led
interior marsh

July 1976
to
October 1976
* 1.3
- 1.2
- 3.2
+ 0.6
- 0.6
» 1.3
+ 2.5
+12.1
* 2.6
INTERIOR
October 1976
to
March 1977
-1.3
+0.6
-1.3
-2.5
0.0
-0.6
—
—
-1.3
MARKER
March 1977
to
July 1977
0.0
--
0.0
+ 0.6
0.0
0.0
—
—
+1.3

July 1976
Net to
Change October 1976
0.0 - 2.5
—
-4.5 - 1.8
-1.9 + 1.3
-0.6 + 0.3
+0.7 + 2.5
+ 3.1
-22.5
+2.6 + 7.6
OUTER MARKER
October 1976 March 1977
to to
March 1977 July 1977
- 0.6 -0.7
..
-15.9
+ 1.9 0.0
- 2.2 0.0
- 1.9 1.9
+ 3.8 -0.6
..


Net
Change
-3.8
-.
--
-0.6
-1.9
+ 2.5
+ 6.3
--
..

-------
                                    Table A-6

           Observed Changes  in  Position  of Shoreline Break-in-Slope  (en)


                             July 1976       October 1976      March  1977
                                 to               to               to          Net
Station     Description     October 1976      March 1977        July  1977      Change
    1


    2


    3


    4


    5
Uncut oiled
river

Cut oiled
river

Uncut unoiled
tributary

Cut unoiled
tributary

Uncut oiled
river bend
-  3.8


-115.6


-  7.6


-  3.2


+  1.3
-1.9


+1.3


-4.5


-3.8


+2.6
+ 3.9






-17.1


+ 8.9


+ 1.2
-  1.8


-209.6


- 29.2


+  1.9


+  5.1
                                        50

-------
- 0
--50
--TOO
    Distance From
   Initial  Location
         (cm)
                                         Station  5
                                         Station  4
                                       *Stat1on  1
                                                              Station 3
                                           • —'	(lost marker) Station 2
-150    .
                                      Time
    July '76
Oct. '76
Mar. '77
July '77
                  Figure A-7.  Position of Bank Edge vs. Time
                                  51

-------
                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before completing)
1. REPORT NO.

 EPA-600/7-78-109
                                                           3. RECIPIENT'S ACCESSION-NO.
4. TITLE AND SUBTITLE
 RESPONSE OF A SALT MARSH  TO  OIL SPILL AND CLEANUP:
 Biotic and Erosional Effects in the Hackensack
 Meadowlands, New Jersey	.	
                                  5. REPORT DATE

                                    June 1978 issuing date
                                  6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
                                                           8. PERFORMING ORGANIZATION REPORT NO.
 Phillip C.  Dibner
9. PERFORMING ORGANIZATION NAME AND ADDRESS
                                                           10. PROGRAM ELEMENT NO.
URS  COMPANY
155  Bovet Road
San  Mateo, California
                                                             EHE 623
94402
11. CONTRACT/qOetKT NO.

  68-03-2160
12. SPONSORING AGENCV NAME AND ADDRESS
 Industrial  Environmental Research  Laboratory-Cin.  OH
 Office  of Research and Development
 U.S.  Environmental Protection Agency
 Cincinatti. Ohio  452fi8		
                                   13. TYPE OF REPORT AND PERIOD COVERED
                                    Final  5/76  -  12/7L
                                   14. SPONSORING AGENCY CODE


                                     EPA/600/12
15. SUPPLEMENTARY NOTES
16. ABSTRACT
   This  study addresses the biological  and erosional response of  portions of the
   Hackensack Meadowlands estuarine marsh to the Wellen Oil Company number 6 crude
   oil spill  of late May 1976, and the  subsequent cleanup operations.   Cleanup
   included  cutting and removal of oiled  grasses of the species Spartina alterm'flora
   from  the  bank of the Hackensack River.   Data were gathered from several loca-
   tions along the river bank and in  the  inner marsh during four  sampling sessions,
   at approximately 4 month intervals,  throughout the year following the spill.
   The productivity of the marsh plants,  the composition of marsh soil  invertebrate
   communities, the presence of oil in  the substrate, and erosional trends were
   monitored.   Results suggest that cutting heavily oiled Spartina soon after
   contamination saved the plants from  dying by root suffocation.   However, the
   foot  traffic associated with cutting is implicated as having made the river
   bank  susceptible to severe erosion by  boat wakes and other sources  of erosive
   energy.   It is concluded that cutting  is only desirable in a limited range of
   circumstances, determined by the characteristics of the contaminating oil, the
   biology of affected plants, and the  time of year.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                              b.lDENTIFIERS/OPEN ENDED TERMS  C. COSATI Field/Group
  Substrates
  Swamps
  Invertebrates
  Grasses
  Erosion
                        Cleanup
                        Hackensack Meadowlands
                        New Jersey
                        Oil  Spill
                        Pragmites  communis
                        Spartina alterniflora
                        Salt Marsh
                43F
                68D
                91A
18. DISTRIBUTION STATEMENT
  Release to public
                                              19. SECURITY CLASS (ThisReport)
                                                 21. NO. OF PAGES

                                                     62
                      20. SECURITY CLASS (Thispage)
                        Unclassified
                                                 22. PRICE
EPA Form 2220-1 (9-73)
                                            52
                                                    U.S. GOVERNMENT HINTING OFFICE: 1978-757-140/1351 Region No. 5-11

-------