WATER POLLUTION CONTROL RESEARCH SERIES
16020 GAG 02/71
             SAMPLING OF
 GLACIAL SNOW  FOR PESTICIDE ANALYSIS
U.S. ENVIRONMENTAL PROTECTION AGENCY

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The. Water Pollution Control Research Series describes the
results and progress in the control and abatement of
pollution in our Nation's waters.  They provide a central
source of information on the research, development, and
demonstration activities in the water research program
of the Environmental Protection Agency, through* inhbuce
research and grants and contracts with Federal, State,
and local agencies, research institutions , and industrial
organizations.

Inquiries pertaining to Water Pollution Control Research
Reports should be directed to the Chief, Publications
Branch (Water), Research Information Division, RSH,
Environmental Protection Agency, Washington, D.C,  20460.

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   SAMPLING OF GLACIAL SNOW FOR PESTICIDE ANALYSIS
                           by
                  Thomas  R.  Stengle
              Department  of  Chemistry
            University of Massachusetts
            Amherst, Massachusetts 01002
                 James J.  Lichtenberg
        Analytical Quality Control Laboratory
                 Water Quality Office
           Environmental Protection Agency
        1014 Broadway, Cincinnati, Ohio 45202
                  Charles  S.  Houston
           Department of Community Medicine
                  College  of  Medicine
              The University  of Vermont
              Burlington,  Vermont 05401
                        for the

           Office of Research and Monitoring

            ENVIRONMENTAL PROTECTION AGENCY



                 Project #16020 GAG

                   February 1971


For sale by the Superintendent of Documents, U.S. Government Printing Office, Washington, D.C., 20402 - Price 38 cents

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                  EPA Review Notice
This report has been revieved by the Environmental Protection
Agency and approved for publication.  Approval does not
signify that the contents necessarily reflect the views and
policies of the Environmental Protection Agency nor does
mention of trade names or commercial products constitute
endorsement or recommendation for use.
                         ii

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                          ABSTRACT
This project was intended to demonstrate the feasibility of
taking snow samples from high altitude snowfields for pesti-
cide analysis, and to develop sampling techniques which
would avoid contamination.  The sampling was to be carried
out in the field, under adverse conditions, with minimal
equipment,  Coincidentally, the DDT level in the sampling
site could be determined.

The sampling was done on the plateau glacier (elevation
17,500 ft.) of Mt. Logan, Y.T., Canada.  The area is remote
from civilization, and the sampling team had to contend
with storms, low temperatures, and the effects of high alti-
tude.  Simple techniques for obtaining samples and shipping
them to the laboratory were tested.  Samples were taken to
a depth of 15 meters to determine whether DDT concentration
varied with the age of the snow.

Analysis showed no DDT within the limit of detectability.
However, over half the samples were contaminated with PCB's
which raised the detectability limit to 10-50 ng/1.  The
limit was 5 ng/1 for the uncontaminated samples.  The PCB
contamination is believed to have come from the sampling
auger, as an analysis of rinsings from it seems to confirm.
It is recommended that more attention be paid to precleaning
the sampling equipment in future work.

 This report was submitted in fulfillment of project
# 16020 GAG under the partial sponsorship of the Water
Quality Office of the Environmental Protection Agency.
                              iii

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                     CONTENTS
Section
   I      Conclusions
   II     Recommendations
   III    Introduction
   IV     Preparations for Field Work
   V   .   Sampling
   VI     Analysis
   VII    Acknowledgments
   VIII   References
 1
 3
 5
 7
11
15
21
23

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                   TABLES
Number                                Page
  I    Collection Data and Analytical
       Results for Snow Samples         13
  II   Results of Trace Metal Analysis
                       vi

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                         SECTION I

                        CONCLUSIONS
     It is highly likely that the plateau glacier (elevation
17,500 ft.) on Mt. Logan, Y.T., Canada is free of contamina-
tion by DDT within the limit of detectability.  The results
of this study are equivocal, since some snow samples were
contaminated with polychlorinated biphenyls, which mask the
presence, if any, of DDT.

     Samples of snow which fell many years ago can readily
be obtained from glaciers, although the sampling must often
be done in remote areas under adverse conditions.  The
samples are suitable for pesticide analysis if the sampling
equipment is well cleaned in advance, and if certain pre-
cautions are followed.  Sampling for trace metals is more
difficult, and cannot be accomplished successfully without
elaborate planning and sophisticated procedures.

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                         SECTION II

                      RECOMMENDATIONS
      The results of this study were clouded because of
contamination of some of the snow samples with PCB's.
This presumably came from the auger used for sampling.  It
is recommended that this work be repeated on a limited
scale with careful precleaning of the equipment in order to
remove this uncertainty.

      In future snow sampling the techniques developed in
this work can be recommended as reliable, inexpensive, and
simple to apply in the field.

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                         SECTION III

                         INTRODUCTION
      Since its introduction a generation ago,  enormous
 quantities of DDT have been used in a variety  of pesticidial
 formulations.  Pears have recently been aroused that much of
 the world has been polluted by residues of this non-biode-
 gradable material*  It is commonly found in surface water,
 and in many animals such as cattle,  fish,  and  even humans.
 The extent of this contamination is  not clear.   It could be
 limited to certain well-defined pathways which lead from
 the original application of the material through a known
 chain of dilutions (with concentration points  such as animal
 fat along the way) to eventual dissipation into concentra-
 tions too small to be detected.   On  the other  hand the
 material could be so widespread that it has become a signif-
 icant constituent of major portions  of the earth,  e.jg.,  the
 atmosphere or the oceans.                          ~

      Although the use of DDT as  a pesticide is  being phased
 out,  the answers to such questions are important,  for the
 DDT system can serve as  a paradigm for other forms of wide-
 spread pollution,  past and to come.   In fact, DDT  is an
 excellent substance for  a model  study.   It is  strictly a
 synthetic chemical,  and  there is  no  natural background
 level.   Since it is not  biodegradable,  its presence can  be
 detected long after it has been applied.   Several  labora-
 tories  have  developed analytical  techniques of  great sensi-
 tivity  for the material.   Finally, as  the  use of DDT is
 phased  out,  its  dissipation can be observed.  Thus,  DDT  is
 an  ideal material  to  study both for  determining the  present
 state  of world pollution and  to observe  the rate of  de-
 pollution as  its use  is  discontinued.

     All of  the  world's  fresh water  is ultimately  traceable
 to  the  atmosphere.  It is  of  considerable  importance to
 determine  if  there are contaminants  in the  atmosphere which
 can be  carried down in precipitation.  The  sampling  of
 glacial  snow  is an intriguing approach to  the problem, for
 it can yield  data  on both  the present and  the past condition
 of the atmosphere.  In many glaciers snow falls from a
 hundred  years  past  lie over one another; one need only bore
 down from  the  surface  to sample the  snow of past years.
 This project  is an attempt to ascertain the feasibility of
 sampling erlacial snow  in the field with minimal equipment,
under adverse  conditions.

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                         SECTION IV

                PREPARATIONS FOR FIELD WORK
      The success of field work is entirely dependent on
careful preparation.  This is particularly true when the
site is a glacier, for the season is short, and one is often
far from civilization.  It may be impossible to procure an
overlooked item or repair malfunctioning equipment.  In this
project careful preparation was especially important, since
the sampling site was very remote, and the prevailing condi-
tions were quite hostile.

      The site chosen for this work was the high plateau
glacier on Mt. Logan at an elevation of 17,500 ft.  For
several years the Arctic Institute of North America has
operated a high altitude research facility at this point
during the summer season.  The DDT sampling was easy to add
on to that laboratory's projects, because the A.I.N.A.'s
personnel have the equipment and experience necessary for
obtaining snow samples at depth in glaciers.

      Mt. Logan lies near the center of the St. Elias range
in the Yukon Territory of Canada.  It is about twenty miles
east of the Alaska-Yukon boundary, and forty miles north of
the Gulf of Alaska.  Mt. Logan is remote from the outposts
of civilization, as it is surrounded by one of the world's
most heavily glaciated areas.  The nearest permanent human
habitation or road is nearly ninety miles away.  Due to the
short growing season, practically no agriculture is practiced
in the Yukon, and massive applications of pesticides are
unknown there.

      In terms of sheer bulk, Logan is said to be the
largest mountain in the world.  The lower section is a mas-
sive rock, roughly ten miles long by four miles wide which
culminates in a plateau at 17,500 ft.  The plateau is
circled by a range of seven peaks, all over 18,000 ft.  The
principal summit reaches to 19,850 ft.  This plateau is the
most favorable spot in North America for a high altitude
laboratory.  It is sheltered from the worst of the high
mountain weather, and is readily accessible to ski-equipped
aircraft.  Less than an hour's flying time away, the Arctic
Institute maintains a permanent base and an airstrip on the
shore of Kluane Lake at 2,600 ft. elevation.  This delight-
ful spot is on the Alaska Highway just 150 miles north of
the city of Whitehorse, the capital of the Yukon Territory.

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 Supplies for the Mt.  Logan site are trucked from Whitehorse
 to Kluane and. then flown onto the mountain by ski plane.

       For collecting  the snow samples,  the Arctic Institute
 purchased a new SIPRE auger with 30 meters of extension
 rods.   In addition another auger was borrowed from the  U.S.
 Army Cold Regions Research and Engineering Laboratory at
 Hanover,  New Hampshire to serve as a backup in case the
 first  instrument was  lost or damaged.   This device was
 designed specifically for sampling of snow and ice by the
 Snow,  Ice,  and Permafrost Research Establishment.   It
 consists  of a barrel  with a bore of three  inches  and a
 length of one meter.   One end is fitted with cutting blades,
 and the other is attached to a tee-bar  handle for turning.
 It is  constructed of  stainless steel and coated with Teflon.
 The cutting blades are of tool steel.   In  use,  the auger is
 turned by hand using  the  tee-bar.   As the  bore hole becomes
 deeper,  extension rods are attached between the auger and
 the handle.   These are one meter long,  made of one inch
 aluminum  pipe,  and fitted at the ends with quick  disconnect
 couplings.   The extension rods  are added on,  one  by one, as
 the auger is  lowered  into the  hole,  and removed again as
 the apparatus  is  raised.

       By  starting with a  brand new auger,  it  seemed that any
 contamination  of  the  samples from  that  source could be  ruled
 out.   This  was  not the case  however, for when the  apparatus
 was  uncrated at Kluane Lake, quantities of  an oily material
 were found  on  both the auger and extension  rods.   This  was
 probably  cutting  oil which had  not  been removed after the
 machine operations.  It was  impossible  to effect a thorough
 cleaning  with  the  facilities at Kluane  Lake,  but most of the
 material  was removed by swabbing the auger  with paper towels
 soaked in Coleman  fuel.  This is a  highly refined  non-leaded
 gasoline  designed  for use  in camp  stoves.   Even after this
 treatment, it was  obvious  that  traces of the  oily  material
 remained.

      Procurement  of the right  sample containers was an
 important aspect of the preparations.  Two  gallon  wide-mouth
 Nalgene jugs were  chosen for use on the glacier.  The mouth
was  large enough so that the 3" snow core could be  slid into
 the  jug directly from the auger without intermediate
 handling.  So long as the core remained frozen, it was  safe
 to store  it in plastic.  However a  test had shown  that,
 given time, water would leach material  (presumably plastici-
zer) from the  jugs.  Therefore, the frozen cores were flown
 to base camp where they were allowed to melt at room temper-
ature: they were then transferred to glass bottler as soon
as possible.  These bottles have been used as water sample

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 containers  by  the  WQO Analytical  Control  Laboratory for  some
 time,  and are  satisfactory  for  shipping samples.  A special
 effort was  made  in the precleaning  of both  jugs and bottles
 because  of  the low levels of pesticide  expected.  The
 cleaning process involved washing with  soap and water.
 rinsing  with tap water followed by  distilled water,  and
 finally  with "distilled in  glass" quality acetone.   These
 preparations were  performed by  personnel  of the WQO Analyti-
 cal Control Laboratory in Cincinnati, Ohio.  Sixteen two-
 gallon jugs and  60 one-quart bottles were sent to Kluane.
 They were not  opened until  used.

       A  number of  other items not directly  involved  in the
 sampling had to  be provided.  A SIPHE snow  kit for determin-
 ing core densities  was borrowed from U.S. Army CRREL.  De-
 contamination  was  not a problem here, since the short core
 sections  used  for  density measurement were discarded after-
 wards.   The other  items needed at the sampling site were:
 a tent for  storage  of equipment, a  shovel for digging the
 initial  pit, notebook and pencils,  labels for the Nalgene
 jugs, and rubber bands and plastic bags.  The plastic bags
were used to cover  the hands of the man handling the auger
and the rubber bands  held them on.                        '

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                         SECTION V

                          SAMPLING
      Samples could not be taken at the high altitude camp,
because of the probability of contamination from generator
and aircraft exhaust.  Therefore, a site was selected 1,000
feet WSW  (the direction of the prevailing winds) of the
camp, and at a slightly higher elevation.  As an additional
precaution to reduce contamination, all trash at the camp
was buried rather than burned.  A four-man tent was set up
at the sampling site to store the auger and other equipment.
Next to it, a pit, one meter in depth by two meters in dia-
meter, was dug to remove the loose overburden of recently
fallen snow.  The drilling took place on the floor of this
pit, and the reported drilling depths are in meters below
this surface.  This pit also gave the drilling party some
protection from the wind while working.

      Precaution was taken to avoid contamination during
sample collection, although the conditions on the mountain
precluded the use of really sophisticated techniques.  Before
collecting any samples, three short holes were drilled and
the cores discarded in the hope of removing any last traces
of foreigh material from the auger.  The drilling team was
made up of three persons.  Two men operated the drill and
attached the extension rods, while the third worked with the
auger itself.  This man wore large polyethylene bags over
his mittens attached by rubber bands.  He alone handled the
auger or touched the core.  Often surgical gloves are used
for such work, but their lack of warmth would have certainly
resulted in frostbite.  Most of the drilling was done at
temperatures between 0° and -10°, and often in the wind.

      The altitude of the drilling site presented a serious
problem.  At 17,500 ft. the barometric pressure is half that
of sea level, and the effects of hypoxia are quite marked.
Although the drilling team was well acclimatized to altitude,
they were quickly exhausted by the heavy work.  Furthermore,
the lack of oxygen seems to intensify the effect of cold.
The psychological effects are equally important*  One's
work is constantly hampered by a feeling of lassitude and an
impairment of Judgement.

      In a typical drilling operation the auger is lowered
into the hole and the extension rods are attached as it
descends.  The actual drilling takes only a few minutes and

                              11

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is not difficult.  Lifting the auger from the hole is very
hard work, and quite tiring.  If one should let the auger
slip when it is near the surface, and most extension rods
have been removed, it would fall to the bottom of the hole.
If the depth were ten meters or greater, it would be impos-
sible to retrieve the auger, and the project would have to
be terminated unless a spare auger were available.

     When the auger is lifted from the hole, the person
with hands bagged in polyethylene grasps it: while the last
extension rod is removed.  Then he tips it upside down to
allow any loose snow to fall out.  The core is removed by
tapping the auger with a piece of wood (bagged in polyethyl-
ene) while it is pointed into the mouth of a Nalgene sample
jug.  If the core is too long to fit into the jug, it can
be broken off between the jug and,auger with one man holding
each.  Once a team becomes familiar with the procedure,
cores can be transferred routinely from auger to sample jug
without touching any other surface.  With each drilling
about 1/3 meter of core was obtained, the rest being loose
snow which was discarded.

     Each sample jug held slightly less than one meter of
core.  The temperature on the high plateau never rises to
freezing, so the samples remained frozen until they were
flown off the mountain.  Thus the danger of contamination by
leaching of the plastic is minimized.  At Kluane Lake the
samples were allowed to melt at room temperature without
local heating.  The process took about 24 hours.  As soon as
it was thawed, the sample was poured into a one quart glass
wide mouth bottle, and the bottle sealed.  Only about one
half the contents of a Nalgene jug was required to fill a
one quart bottle, and the rest of the sample was discarded.
In the most favorable cases the samples touched no surfaces
other than the auger, the Nalgene jug, and the glass bottle.
Other samples had to be touched with polyethylene bagged
hands in the process of transferring it from the auger to
the jug.

     There are several possible sources of contamination
in the sampling procedure.  Despite the attempt to clean
the auger, it is clear that quantities of the oily material
remained on it.  During the course of the drilling, bits of
the Teflon coating began to flake off the auger.  This ma-
terial may be present in some samples, although it should
have been discarded with the loose snow and not included
with the core.  In order to operate the drilling apparatus
properly, it is necessary to cover the drilling platform
with a board that has a hole large enough for the auger to
pass through.  Even though the board was covered with mask-
ing tape and polyethylene sheeting, splinters fell into the

                              12

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drill hole.  They should have been discarded with the loose
snow, but it is possible that some found their way into the
sample.

      Several of the early cores showed a dark band on the
outside about i inch from the cutting head of the auger.
This discoloration was present only on the surface of the
sample.  In all cases but one, it was broken off and dis-
carded.  As a control, it was retained on sample #5-  It
could have been present to a lesser extent in other samples,
and gone by unnoticed.  In any event it had no effect on
sample #5, as shown by analysis.

      After the samples were thawed and placed in glass
bottles, some solid material was observed in them.  If a
sample was shaken and held toward the light, one could see
a few very small pieces of fuzz which resembled bits of
wool.  Neither the source nor the nature of this material
has been ascertained.  The drilling team wore outer clothes
of nylon or nylon-cotton mix.  Although they did occasional-
ly wear wool gloves, they took great care not to touch the
samples, either directly or indirectly, except with hands
encased in plastic bags.

      At the end of the drilling, one sample was deliberate-
ly mishandled as a control.  The drilling team rubbed it
with gloved hands and against their pants.  If there is any
contamination arising from clothing, this control should
show at least 100 times as much as the other samples.
                              13

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                          SECTION VI

                           ANALYSIS
       As soon as possible after the termination of the Mt.
 Logan project, the samples contained in glass bottles were
 sent to the Analytical Control Laboratory,  WQO, Cincinnati,
 Ohio for analysis.  This was performed using vapor phase
 chromatographic techniques developed at that laboratory.

       DDT was not detected in any of the samples.   The lower
 limit of detectability for DDT was approximately 5 ng/1 for
 eight of the samples.   Due to interference,  apparently from
 polychlorinated biphenyls (PCB's), 10 to 50  ng/1 of DDT
 could have been present in the remaining twelve samples and
 not  been detected.  Efforts to remove the PCB's from  the
 extract to allow for more sensitive determination of  DDT
 were only partially  successful.   Thus the results  are in
 part inconclusive.  It seems highly likely however, that the
 DDT  concentration in all samples  was less than 5 ng/1 on the
 basis of the eight samples which  did not show PCB  interference,

       The source of  the apparent  PCB's  (Aroclors 1260 or
 1262)  is not known for certain, although a residue of the
 oily substance on the  auger is a  likely  suspect.   There is
 no pattern of occurrence of the PCB's in the  samples.   Three
 scattered samples  contained significant  quantities of what
 appears  to be Aroclor  1260  or 1262.   Samples  1  through 3 and
 5 through 10  contained varying amounts of a similar,  but not
 identical material.  The Kluane Lake  sample and  the five
 samples  received  in  the  second shipment  (#15,16,17,18,20)
 did  not  contain such material.  All possible  sources  of
 PCB1s  in the  laboratory  were  thoroughly  checked, and  there
 is no  reason  to  suspect  that  this material was introduced
 during the analyses.

       The dark band  retained  in core  sample 5 had no appar-
 ent  effect  on the  analytical  results.  Sample 20, deliber-
ately handled  so as  to contaminate it, gave a peak for
p,p'-DDT  on one gas  chromatographic column.   However,  this
was not  confirmed by analysis on a second column.

      All  samples produced a broad initial peak with 3 to 4-
distinct  early eluting- peaks which were more or less typi-
cal of the distilled water check that was made on the  plastic
 jugs.  In addition, samples 1 through l*f gave a high general
background response to the electron capture  detector.


                              15

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      As a matter of curiosity, the pH (which may not
correspond to the original pH) of randomly selected samples
from the first shipment (sample 1 through 14 plus Kluane
Lake) was measured on arrival in the laboratory as was
that of the five samples that arrived in the second shipment.
Sample 20, deliberately "contaminated" was low with pH 6.1.
The Kluane Lake sample was high with pH 8.0  All others
ranged from 6.3 to 6.9.

      In an attempt to fix the source of the FOB contamina-
tion, the auger and one of the extension rods were sent to
the laboratory where they were sampled by rinsing with
hexane.  The extension rod clearly contained traces of an
oily material inside the stainless steel fitting at the end.
This appeared to be a cutting oil.  The chromatogram showed
a series of early eluting peaks, but no traces of Aroclors.
These early peaks would not have obscured the presence of
DDT.  None of the material from the rods was found in the
snow samples.  The rinsings from the auger gave a chromato-
gram which was rich in peaks, as well as a high general
background response.  A similar high background was observed
in snow samples l-l4.  This fraction also contained a quan-
tity of halogenated material, which was an Aroclor, a mix-
ture of Aroclors, or a similar substance.  Its chromatogram
resembled, but did not exactly reproduce that of Aroclors
1260 and 1262.  Even though an exact match cannot be made,
it is reasonable to conclude that the auger was responsible
for the contamination of the snow sample.  The material is
a complex mixture, and it is possible that some components
were preferentially lost from the auger as drilling proceed-
ed.  This contention is supported by the fact that the last
five snow samples showed no PCB contamination.

      One sample was obtained for trace metal analysis.  It
was taken as a snow core extending from a depth of l£ to 2&
meters.  It was stored in a Nalgene jug and acidified with
distilled nitric acid as soon as it melted.  It remained in
the plastic container until use.  Simultaneous analysis for
19 elements was performed on a direct reading emission spec-
trometer.  Pour elements were detected: boron at 4 ng/ml,
cadmium at 9 ng/ml, chromium at 2 ng/ml, and iron at 4? ng/ml,
The high levels of cadmium and especially iron suggest con-
tamination from the alloy steels of\ the auger.  This is not
surprising; other workers have notedx that it is extremely
difficult to avoid metal ion contamination when steel sam-
pling tools are used (1).
                             16

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Method of Analysis (2)
      Each sample was transferred to a two liter separatory
funnel.  The sample bottle was rinsed with 60 ml. of hexane
which was then used to extract the sample.  This operation
was repeated a second and a third time.  The combined ex-
tracts were dried by passing through a column of anhydrous
sodium sulfate and collected in a Kuderna-Danish (K-D)
flask.  They were concentrated in the K-D to- about 6 ml. on
a steam bath and finally to a minimum of 2 ml. on a warm
water bath.  Up to 10 microliters was injected for gas
chromatographic analysis on two different columns.

      Gas chromat©graphic conditions were as.follows:
1.  A Microtek Model 179 equipped with a Ni0-^ detector, an
aluminum column 6' x i" O.D. packed with Gas-Chrom Q (60/80
mesh) coated with 5% OV-17, and a nitrogen carrier flow of
100 ral./min.  Temperatures were: injection port, 250° C.;
column oven, 205° C.; and detector, 360° C.
2.  A Perkin-Elmer Model 900 equipped with a tritium
parallel plate electron capture detector, an aluminum
column 81 x 1/8" O.D. packed with Gas-Chrom Q (60/80 mesh)
coated with $% QP-1 plus 3# Dow-200, and a nitrogen carrier
flow of 40 ml./min.  Temperatures were: injection port,
250° C.; column oven, 185° C,; and detector, 205° C.
                              17

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                          TABLE I
  COLLECTION DATA AND ANALYTICAL RESULTS FOR SNOW SAMPLES
Sample No.
(Lab. No.)

   I (508)
   2 (509)
   3 (510)
   4 (511)
   5 (512)
   6 (513)
   7 (514)
   8 (515)
   9 (516)
  10 (517)
  11 (518)
  12 (519)
  13 (520)
  14 (521)
  15 (539)
  16 (5^1)
  17 (540)
  18 (542)
  20 (543)
KLAb (506)
KLAa (507)
7-13
7-13
7-13
7-13
7-13
7-13
7-16
7-16
7-16
7-16
7-20
7-20
7-20
7-20
7-20
7-20
7-21
7-21
8-5
7-20
7-20
        Depth
       (meters)
DDT

ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
          PCS    £H
P
P
P
+
P
P
P
P
P
P
ND
ND
+
ND
ND
ND
ND
ND
ND
                6.5
                 -
                 -
                6.4
                6.3
                 -
                6.4
                 -
                6.4

                6.9
                6.5
                6.6
                6.4
                6.1
                8.0
            Density
            (gm/oc)
                         0.39
 0.55
0.54
not analyzed
Notes;

Depths are approximate and refer to the average depth of
the core.

ND - Not detected.

( + ) - Aroclor 1260 or 1262

P - Similar but not identical to Aroclor 1260 or 1262.

pH values determined after arrival at Cincinnati; they may
not reflect the original pH values.

Sample 20 was deliberately contaminated by handling.

Samples KLAa and KLAb were taken from the waters of Kluane
Lake as a control.
                              18

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                          TABLE II
               RESULTS OP TRACE METAL ANALYSIS
          Metal

          Zinc
          Cadmium
          Arsenic
          Boron
          Phosphorous
          Iron
          Molybdenum
          Manganese
          Aluminum
          Beryllium
          Copper
          Silver
          Nickel
          Cobalt
          Lead
          Chromium
          Vanadium
          Barium
          Strontium
ND
9 ppb
ND
b ppb
ND
ty ppb
ND
ND
ND
ND
ND
ND
ND
ND
ND
2 ppb
ND
ND
ND
less than 2 ppb

less than 28 ppb

less than 10 ppb
less
less
less
less.
less
less
less
less
less
than
than
than
than
than
than
than
than
than
4 ppb
1 ppb
4 ppb
0.02 ppb
1 ppb
0.2 ppb
2 ppb
2 ppb
^ ppb
less than ^ ppb
less than 1 ppb
less than 1 ppb
Notes:
   —1--1                          ^

 Results are for total metals  (dissolved + suspended).

Results are given as nanograms per milliliter.
                             19

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                        SECTION VII

                      ACKNOWLEDGMENTS
     The Arctic Institute of North America provided logistic
support for the Mt. Logan Project.  Special recognition is
given to the Institute's glacier pilot, Mr. Philip P. Upton,
whose flying skill was indispensable to the existence of
the high camp.

     Thanks go to the support team of the Logan high camp
who volunteered their help with the arduous work of snow
coring.

     This project was supported by the Water Quality Office
of the Environmental Protection Agency.  James W. Eichelberger
of the Analytical Control Laboratory, Cincinnati, Ohio,
gave invaluable assistance with the DDT analyses.  The metal
analyses were performed by John P. Kopp.

     The help of Carol Harden in preparation of the manu-
script is gratefully acknowledged.
                              21

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                       SECTION VIII


                        REFERENCES
1.  Murozumi, M.,  Chow, T.J.,  and Patterson, C.,  "Chemical
    Concentrations of Pollutant Lead Aerosols,  Terrestrial
    Dusts and Sea Salts in Greenland and Antarctic Snow
    Strata," Geochimica et Cosmochimica Acta. 33.  pp 124-7-
    "PWPCA Method for Chlorinated Hydrocarbon Pesticides
    in Water and Wastewater,"  U.S. Department of the
    Interior, Federal Water Pollution Control Administra-
    tion,  Division of Water Quality Research, Analytical
    Control Laboratory,  Cincinnati, Ohio,  April, 1969.
                            23

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   SELECTED WATER
   RESOURCES ABSTRACTS
   INPUT TRANSACTION FORM
                                              1. Report No,
   4. Title
           "Sampling of Glacial Snow for  Pesticide Analysis"
   7. Author(s)    Thomas R. Stengle  (1)
                 James J. Lichtenberg  (2)
  	f                     "
                                          i 3. Accession No.

                                          ;w

                                          "jr.-^jjdrtJJatff / "/v-v";'"
                                          I-'*?"'**  '."Vv>.  £/ "< WASHINGTON, D. C. 20240
                                      institution AQC Laboratory, EPA, Cincinnati,  Ohio
WRSIC 102 (REV. JUNE 1B7l)
                                                                                 SPO 913.261

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