FINAL REPORT
   RECALCULATION OF SCREENING LEVEL CONCENTRATIONS
FOR NONPOLAR ORGANIC CONTAMINANTS IN MARINE SEDIMENTS
                     Prepared by


Jerry M. Neff, Jack Q.  Word,  and Thomas C.  Gulbransen



                    December  1987
                     Prepared for
         U.S. Environmental Protection Agency
           Criteria and Standards Division
       Office of Water Regulation and Standards
                   Washington, D.C.
                     Submitted by
                       BATTELLE
       Washington Environmental Program Office
                 2030 M Street, N.W.
                   Washington, D.C.

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                          INTRODUCTION

     In Work Assignment 56,  Task IV,  Battelle evaluated the
screening level concentration (SLC)  approach to developing
sediment quality criteria for nonpolar organic contaminants  in
freshwater and marine sediments.  The results of that effort
were summarized in a final report titled,  Sediment Quality
Criteria Methodology Validation: Calculation of Screening Level
Concentrations from Field Data.  The  report was submitted to  EPA
in July 1986.  In that report, Battelle identified a lack of
standardization of taxonomic nomenclature and identifications of
benthic infauna among the several large field databases used for
calculating marine SLCs.  Therefore,  it was decided to review
the marine databases to identify synonymies and inappropriate
taxonomic distinctions, and to eliminate data sets in which the
taxonomy was suspected of being inaccurate. This work was
performed as a task in Work Assignment 77.
     The objectives of this task were to

       o  Edit the database used to calculate SLC
          values for marine sediments
       o  Remove any questionable data sets  from the
          SLC database
       o  Based on the revised data base,  recalculate
          SLCs for selected nonpolar organic
          contaminants in marine sediments.
                     MATERIALS AND METHODS

     The species lists from the data sets for  southern
California and Puget Sound were reviewed. Species names  that
were synonymous were combined.  In addition, the reasonableness
and rigor of the overall taxonomic identifications were  reviewed
on the basis of knowledge of  the taxonomy of the benthic fauna
of the study areas.  Inappropriate taxonomic distinctions were
identified and corrected.

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     Any suspect species lists were noted and the data sets
containing them were eliminated from the database.  Species that
were identified as synonymous were combined,  inappropriate
taxonomic distinctions were corrected,  and this information was
entered into the database. In addition, the two southern
California data sets were reviewed and all species that occurred
in both data sets were combined if the resulting combination
produced the minimum of 20 observations required for calculating
a species screening level concentration (SSLC).
     The revised database was then used to calculate SSLCs,
SLCs, and the 95 percent confidence intervals for the SLCs for
12 nonpolar organic contaminants in marine sediments. The
methods used were those described in detail in the 1986 final
report (Neff et al., 1986). The compounds were total DDT, total
polychlorinated biphenyls (PCBs), and 10 polycyclic aromatic
hydrocarbons (acenaphthalene, naphthalene, fluorene,
phenanthrene, anthracene, -fluoranthene, benz(a)anthracene,
chrysene, pyrene, and benzo(a)pyrene).
                            RESULTS

     Several synonymies or inappropriate taxonomic distinctions
were identified in the data sets. The corrected species were
combined under the currently accepted taxonomic designation.  The
following synonymies or inappropriate taxonomic distinctions
were identified and corrected:
       o  Ampharete. arctica, A. goesi, A.  labrops, A.
          acutifrons
       o  Goniada brunnea, G. maculata
       o  Mediomastus ambiseta, M.  spp.,  M.
          californierisis
       o  Prionospio cirriferra,  Minuspio cirriferra.

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     Serious technical problems were identified in the taxonomy
of the Puget Sound "Eight Bay Study."  Therefore,  this data set
was deleted from the database.  This resulted in loss of some
data for 20 species and loss of some SSLC values for all
contaminants except DDT, pyrene, and benzo(a)pyrene.
     By combining species that were present in the two southern
California data sets, it was possible to calculate SSLC values
for five additional species. These SSLCs then were added to
those already in the database and were used in the recalculation
of SLCs.
     The SLC values derived from the revised database are
summarized in Table 1. The SSLC values and resulting frequency
distribution curves are presented in Attachments 1 through 12.
In all but one case, the SLC values calculated with the revised
database were higher than the values calculated with the old
database.fThe exception was P.CBs.  The SLC value for PCBs
                             ;*                       +£'
dropped slightly from 4.26 tor 3.66 j/g/g organic carbon'.  None of
the changes should be considered statistically significant,
given the relatively large 95 percent confidence intervals for
most values.
     Revision of the database allowed us to calculate SLCs for
three compounds for which SLCs were not calculated  as part of
the original work assignment. These were acenaphthalene,
fluorene, and anthracene. SLCs  for  these compounds  were in the
range of 4.74 to 16.3 vg/g organic  carbon.
                           DISCUSSION

     Quality control review of the data sets used  to  calculate
SLCs resulted in the combination of several entries  for
synonymous or misidentified species in the species lists,
addition of species occurrences for species that occurred  in two
data sets, and elimination of one questionable  data  set. These
changes in the database resulted in small changes  in  the
calculated SLC values  for several nonpolar organic contaminants

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TABLE 1.  OLD  AND  RECALCULATED  SCREENING  LEVEL  CONCENTRATIONS  FOR
          NONPOLAR  ORGANIC COMPOUNDS IN MARINE  SEDIMENTS (ALL  VALUES
          ARE pg/g ORGANIC CARBON)
Compound
DDT
PCB
Acenaphthalene
Naphthalene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Benz( a) anthracene
Chrysene
Pyrene
Benzol a)pyrene
Screening
Old Value
42.8
4.26
—
36.7
—
25.9
—
43.2
26.1
38.4
43.4
39.6
Level Concentration
New value
50.5
3.66
4.74
41.4
10.1
36.8
16.3
64.4
26.1
38.4
66.5
39.7

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in marine sediments.  There was no change  for  three  compounds,  a
decrease in the SLC for one compound,  and an  increase  in  the  SLC
for five compounds. None of the changes were  sufficiently large
that the new value was outside the 95  percent confidence
interval for the old value. Therefore,  the differences were
considered statistically insignificant. The largest change was
an approximately 50 percent increase in the SLC for pyrene from
43.4 to 66.5 j/g/g organic carbon. Most of the changes  were
between 10 and 15 percent.
     These results indicate that the SLC  approach is not
particularly sensitive to minor changes in the database from
which SLCs are calculated. As the number  of observations
                               »
increases and the number of SSLCs used to calculate SLCs  grows,
the sensitivity of the calculated SLC values to small  changes in
the database will decrease further.
     These results confirm that the SLC approach to sediment
criteria development or validation has basic technical validity.
Its strengths include the use of real field data on the
cooccurence of contaminants and infaunal  animals in sediments
and the lack of any a priori assumptions  about the relationship
between the distribution of benthic fauna and the concentrations
of contaminants in sediments. These attributes of the SLC
approach make it an ideal candidate for a method to validate
sediment quality criteria developed by more theoretical
approaches, such as equilibrium partitioning.
     The SLC approach should be further developed to  improve  the
statistical rigor of the SLC calculation process and  the
technical validity of the SLC values generated. Several
variations of different aspects of the method  for calculating
the SLC should be tried and evaluated by sensitivity  analysis.
As many additional data sets as possible should be added  to  the
database. A large number of additional data  sets have been
identified and it may be possible  to increase  the number  of
stations in the database to about  1000.  All  data  sets should be
subjected to rigorous quality  assurance  review. Entry of  the

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complete database into a sophisticated computer database
management system will facilitate combination of species lists
from different data sets,  entry of new data sets as they become
available, and performance of sensitivity and statistical
analyses on the SLC calculation method and the resulting SLC
values.
     It is essential that EPA has an independent method to
validate the EP approach or other approach for derivation of
sediment quality criteria.  The SLC approach is the ideal one
for that purpose.
                        LITERATURE CITED
Neff, J.M., O.J. Bean, B.W. Cornaby, R.M. Vaga, T.C. Gulbransen,
      and J.A. Scanlon. 1986. Sediment Quality Criteria
      Methodology Validation: Calculation of Screening Level
      Concentrations from Field Data. Report to U.S.
      Environmental Protection Agency, Criteria and Standards
      Division, Office of Water Regulation and Standards,
      Washington D.C. 60 pp.

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                             ACENAPHTHALENE


Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
SLC =
Cumulative
Freq. U)
4.8
9.5
14.3
19.0
23.8
28.6
33.3
38.1
42.9
47.6
52.4
57.1
61.9
66.7
71.4
76.2
81.0
85.7
90.5
95.2
100.0
4.739 ug/gTOC
SSLC
(ug/g Org.C)
4.706
5.479
5.714
5.714
6.522
6.522
6.522
7.190
7.190
7.190
7.190
7.190
7.391
7.391
7.391
9.286
9.286
13.861
13.861
13.861
15.702
[0.0 -
No. of
Obser .
24
25
21
28
23
41
49
33
53
24
51
25
47
47
49
28
28
26
27
25
. 20
                                            Organism
                                           COMPSOMYAX SUBDIAPHANA
                                           PRAXILLELLA GRACILIS
                                           PECTINARIA CALIFORNIENSIS
                                           AMPHIODIA (AMPHISPINA) URTICA
                                           EUCHONE INCOLOR
                                           CAPITELLA CAPITATA
                                           AXINOPSIDA SERICATA
                                           NEPHTYS CORNUTA FRANCISCANA
                                           HACOMA CARLOTTENSIS
                                           GONIAOA BRUNNEA
                                           EUPHILOMEDES CARCHARODONTA
                                           ARMANDIA BREVIS
                                           PRIONOSPIO STEENSTRUPI
                                           NEPHTYS FERRUGINEA
                                           GLYCERA CAPITATA
                                           PLATYNEREIS BICANALICULATA
                                           GLYCERA AMERICANA
                                           SPIOCHAETOPTERUS COSTARUM
                                           PHOLOE MINUTA
                                           PARVILUCINA- TENUISCULPTA
                                           SPIOPHANES BERKELEYORUM
(ug/gTOd
     14.0 +
     10.5 +
      7.0 +
      3.5+
           +-
           0
                 ACENAPHTHALENE vs. CUMULATIVE FREQUENCY
                       * *  *
                * *  *
• - + -
20
                                   *  *   *
                                          * *  *
           *  *   *  *   *
        60        80
Cumulative Frequency
—+
 100
                             Attachment 1

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                               ANTHRACENE
SLC = 16.316 ug/gTOC [0.0
Cumulative SSLC No. of
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Freq. ( ', )
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
(ug/g Org.C)
15.953
18.235
19.130
19.595
19.595
19.595
20.000
20.000
20.183
20.183
20.183
21.739
21.739
21.739
21.739
23.288
23.288
23.288
27.273
27.273
27.273
27.273
42.353
Obser.
25
23
20
20
35
25
21
52
26
53
43
25
50
56
52
50
21
30
26
30
27
29
30
                                        -  19.596) a-0.026
                                           Organism
                                          ARMANDIA BREVIS
                                          EUCHONE INCOLOR
                                          PRIONOSPIO  CIRRIFERA
                                          PHYLLODOCE  HARTMANAE
                                          NEPHTYS CORNUTA  FRANCISCANA
                                          COMPSOMYAX  SUBDIAPHANA
                                          SPIOPHANES  BERKELEYORUM
                                          AXINOPSIDA  SERICATA
                                          GONIAOA BRUNNEA
                                          EUPHILOMEDES  CARCHARODONTA
                                          CAPITELLA CAPITATA
                                          PRAXILLELLA GRACILIS
                                          NEPHTYS FERRUGINEA
                                          HACOMA CARLOTTENSIS
                                          GLYCERA CAPITATA
                                          PRIONOSPIO  STEENSTRUPI
                                          PECTINARIA  CALIFORNIENSIS
                                          GLYCERA AMERICANA
                                          SPIOCHAETOPTERUS COSTARUM
                                          PHOLOE MINUTA
                                          PARVILUCINA TENUISCULPTA
                                          AMPHIODIA  (AMPHISPINA) URTICP
                                          PLATYNEREIS BICANALICULATA
(ug/gTOC)

     40.0 +
     32.0 +
     24.0 +
                   ANTHRACENE vs.  CUMULATIVE FREQUENCY
                  * * * *
     16.0+   *
         I
                                                    *  * * *
                * * *
                                     * * *  *
                          * *  * * *
                    20
40        60        80
  Cumulative Frequency
100
                             Attachment 2

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                            BENZ(A)ANTHRACENE
SLC = 26.119 ug/gTOC (0.0 - 40
Cumulative
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21-
22
23
Freq. ( °, )
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
SSLC
(ug/g Org.C)
24.348
35.477
35.477
40.952
41.322
42.466
44.118
44.118
44.118
44.118
44.118
44.118
47.647
47.647
47.647
47.647
47.647
47.647
47.945
51.765
51.765
51.802
51.802
No. of
Obser. 0
20 PR
21 SP
25 AR
26 GO
26 SP
52 AX
56 MA
52 GL
53 EU
28 EU
25 CO
49 CA
57 PF
25 PF
21 PE
27 P*
50 NE
35 NE
29 A*
38 PE
30 GI
30 PI
20 PF
                                                  a=0.026
                                           Organism
                                           PRIONOSPIO  CIRRIFERA
                                           SPIOPHANES  BERKELEYORUM
                                           ARMANDIA BREVIS
                                           GONIADA BRUNNEA
                                           SPIOCHAETOPTERUS  COSTARUM
                                           AXINOPSIDA  SERICATA
                                           MACOMA CARLOTTENSIS
                                           GLYCERA CAPITATA
                                           EUPHILOMEDES  CARCHARODONTA
                                           EUCHONE  INCOLOR
                                           COMPSOMYAX  SUBDIAPHANA
                                           CAPITELLA CAPITATA
                                           PRIONOSPIO  STEENSTRUPI
                                           PRAXILLELLA GRACILIS
                                           PECTINARIA  CALIFORNIENSIS
                                           PARVILUCINA TENUISCULPTA
                                           NEPHTYS  FERRUGINEA
                                           NEPHTYS  CORNUTA FRANCISCANA
                                           AMPHIODIA  (AMPHISPINA) URTICA
                                           PHOLOE MINUTA
                                           GLYCERA AMERICANA
                                           PLATYNEREIS BICANALICULATA
                                           PHYLLODOCE  HARTMANAE
(ug/gTOC)
     48.0 +
     40.0 +
     32.0 +
      24.0 +
           + -
           0
               BENZ(A)ANTHRACENE vs. CUMULATIVE FREQUENCY
                    * *
                                                       * * * *
                                       * *  *****
                          * *  * * * *
20
40        60        80
  Cumulative Frequency
—+
 100
                              Attachment 3

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                             BENZO(A)PYRENE
SLC =
Cumulative
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Freq. ( \)
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
55.2
59.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
39.667 ug/gTOC [0.0 -
SSLC Mo. of
(ug/g Org.C)
39.604
40.000
46.552
46.795
49.315
49.315
50.000
50.000
50.000
50.000
50.000
50.588
52.910
52.910
52.910
55.372
55.372
55.372
55.372
61.644
61^.644
66\667
137.387
Obser .
21
20
49
26
25
28
25
51
52
25
51
34
56
55
25
37
21
26
49
29
29
29
20
                                            Organism
                                           SPIOPHANES BERKELEYORUM
                                           PRIONOSPIO CIRRIFERA
                                           CAPITELLA CAPITATA
                                           SPIOCHAETOPTERUS COSTARUM
                                           GONIADA BRUNNEA
                                           EUCHONE INCOLOR
                                           PRAXILLELLA GRACILIS
                                           GLYCERA CAPITATA
                                           EUPHILOMEDES CARCHARODONTA
                                           COMPSOMYAX SUBDIAPHANA
                                           AXINOPSIDA SERICATA
                                           NEPHTYS CORNUTA FRANCISCANA
                                           PRIONOSPIO STEENSTRUPI
                                           HACOMA CARL-Q.TTENSIS
                                           ARMANDIA B&ELVIS
                                           PHOLOE MINU^A
                                           PECTINARIA CALIFORNIENSIS
                                           PARVILUCINA TENUISCULPTA
                                           NEPHTYS FERRUGINEA
                                           GLYCERA AMERICANA
                                           AMPHIODt^(AMPHISPINA)  URTICA
                                           PLATYNERE>IS BICANALICULATA
                                           PHYLLODOCE HARTMANAE
(ug/gTOC)
      120 +
       90 +
       60 +
                 BENZO(A)PYRENE vs. CUMULATIVE FREQUENCY
                                        *  *
                  ******  ****
1 * *
1
30 +
0



20



'10



60



80



10
                               Cumulative  Frequency
                              Attachment 4

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                                CHRYSENE
               SLC = 38.395 ug/gTOC [0.0 -  60.847)  a=*0.026

Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Cumulative
Freq. Co)
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
SSLC
(ug/g Org.C)
35.652
52.893
57.143
60.847
62.084
62.084
62.084
63.694
63.694
64.706
64.706
64.706
68.966
68.966
69.863
69.863
75.314
75.314
76.471
76.471
76.471
76.471
76.471
No. of
Obser .
20
21
26
25
20
48
51
57
28
50
55
52
25
51
25
21
35
25
30
38
26
29
29
                                            Organism
                                           PRIONOSPIO CIRRIFERA
                                           SPIOPHANES BERKELEYORUH
                                           GONIADA BRUNNEA
                                           ARMANDIA BREVIS
                                           PHYLLODOCE HARTMANAE
                                           CAPITELLA CAPITATA
                                           AXINOPSIDA SERICATA
                                           PRIONOSPIO STEENSTRUPI
                                           EUCHONE INCOLOR
                                           NEPHTYS FERRUGINEA
                                           MACOMA CARLOTTENSIS
                                           EUPHILOHEDES CARCHARODONTA
                                           SPIOCHAETOPTERUS COSTARUM
                                           GLYCERA CAPITATA
                                           PRAXILLELLA GRACILIS
                                           PECTINARIA CALIFORNIENSIS
                                           NEPHTYS CORNUTA FRANCISCANA
                                           COMPSOMYAX SUBDIAPHANA
                                           PLATYNEREIS BICANALICULATA
                                           PHOLOE MINUTA
                                           PARVILUCINA TENUISCULPTA
                                           GLYCERA AMERICANA
                                           AMPHIODIA (AMPHISPINA) URTICA
(ug/gTOC)

       72 +
       60 +
       48 +
       36 +
         I
           +•
           0
                    CHRYSENE vs. CUMULATIVE FREQUENCY
                                                    *  * * * *
                            * *
                                       * *  * *
                            *  * * * *
                      * * *
20
40        60        80
 Cumulative Frequency
100
                               Attachment  5

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                                   DDT
               SLC = 50.488 ug/gTOC [0.0  -  113.684)  a=0.02
Cumulative SSLC
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Freq. (\)
4.8
9.5
14.3
19.0
23.8
28.6
33.3
38.1
42.9
47.6
52.4
57.1
61.9
66.7
71.4
76.2
81.0
85.7
90.5
95.2
100.0
(ug/g Org.C)
50.488
50.438
68.696
113.684
137.692
137.692
202.441
207.917
222.389
954.033
1136.319
1146.004
1186.331
1260.058
1392.128
1407.287
1511.990
1816.188
1999.961
2069.586
2069.586
No. of
Obser .
21
27
29
21
20
29
25
20
22
62
44
22
79
45
86
61
101
51
44
37
57
                                            Organism
                                           AMPELISCA  BREVISIMULATA
                                           AMPHIODIA  (AMPHISPINA)  URTICA
                                           EUPHILOMEDES  CARCHARODONTA
                                           HETEROPHOXUS  OCULATUS
                                           STHENELANELLA UNIFORMIS
                                           COMPSOMYAX SUBDIAPHANA
                                           GONIADA BRUNNEA
                                           CHLOEIA PINNATA
                                           GNATHIA CRENULATIFRONS
                                           PECTINARIA CALIFORNIENSIS
                                           MEDIOMASTUS AMBISETA
                                           PRIONOSPIO CIRRIFERA
                                           AXINOPSIDA SERICATA
                                           PARAPRIONOSPIO PINNATA
                                           GLYCERA CAPITATA
                                           PRIONOSPIO STEENSTRUPI
                                           PARVILUCINA TENUISCULPTA
                                           MACOMA CARLOTTENSIS
                                           CAPITELLA CAPITATA
                                           SPIOPHANES BERKELEYORUM
                                           TELLINA CARPENTERI
(ug/gTOC)


     1800 +
     1200 +
      600 +
        0+   *  *
           +•
           0
                      DDT vs. CUMULATIVE FREQUENCY
                            * * *
                  *  * * *
                                    *   * *
20
40        60        80
 Cumulative Frequency
100
                               Attachment  6

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                                FLUORENE
SLC = 10.123 ug/gTOC [0.0
Cumulative SSLC No. of
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Freq. ( 1 )
4.8
9.5
14.3
19.0
23.8
28.6
33.3
38.1
42.9
47.6
52.4
57.1
61.9
66.7
Tl.4
<&g.2
81.0
85.7
90.5
95.2
100.0
( ug/g Org.C)
10.092
10.811
12.353
13.223
13.223
13.223
13.223
13.366
13.366
13.366
13.366
13.366
13.529
13.529
13.529
13.529
13.529
18.824
18.824
18.824
18.824
Obser .
24
24
49
21
53
51
42
20
26
49
47
23
25
25
33
49
24
27
28
28
28
                                         -  13.233)  a=0.02
                                            Organism
                                           GONIADA  BRUNNEA
                                           ARMANDIA BREVIS
                                           AXINOPSIDA SERICATA
                                           PECTINARIA CALIFORNIENSIS
                                           MACOMA CARLOTTENSIS
                                           EUPHILOMEDES  CARCHARODONTA
                                           CAPITELLA  CAPITATA
                                           SPIOPHANES BERKELEYORUM
                                           SPIOCHAETOPTERUS COSTARUM
                                           PRIONOSPIO STEENSTRUPI
                                           NEPHTYS  FERRUGINEA
                                           EUCHONE  INCOLOR
                                           PRAXILLELLA GRACILIS
                                           PARVILUCINA TENUISCULPTA
                                           NEPHTYS  CORNUTA FRANCISCANA
                                           GLYCERA  CAPITATA
                                           COMPSOMYAX SUBDIAPHANA
                                           PLATYNEREIS BICANALICULATA
                                           PHOLOE MINUTA
                                           GLYCERA  AMERICANA
                                           AMPHIODIA (AMPHISPINA) URTICA
(ug/gTOC-)


     17.5 +
     15.0 +
     12.5 +
     10.0+
                    FLUORENE vs. CUMULATIVE FREQUENCY
                     * * *
                                                      * *  * *
                              * *  * *  * * *  * *
                    20
40        60        80
 Cumulative Frequency
—+
 100
                               Attachment 7

-------
                              FLUORANTHENE


Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
SLC =
Cumulative
Freq. CO
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
64.434 ug/gTOC [0.0 -
SSLC
(ug/g Org.C)
64.286
65.217
66.138
81.081
81.651
81.651
111.765
111.765
124.658
124.658
124.658
124.658
124.658
129.412
129.412
129.412
135.294
135.294
135.294
135.294
146.552
164.384
164.384
No. of
Obser .
22
20
25
20
26
27
48
52
58
51
55
53
28
36
52
25
25
39
21
27
31
29
29
                                            Organism
                                           SPIOPHANES  BERKELEYORUH
                                           PRIONOSPIO  CIRRIFERA
                                           ARMANDIA BREVIS
                                           PHYLLODOCE  HARTMANAE
                                           SPIOCHAETOPTERUS COSTARUM
                                           GONIADA BRUNNEA
                                           CAPITELLA CAPITATA
                                           AXINOPSIDA  SERICATA
                                           PRIONOSPIO  STEENSTRUPI
                                           NEPHTYS FERRUGINEA
                                           MACOMA CARLOTTENSIS
                                           EUPHILOMEDES CARCHARODONTA
                                           EUCHONE INCOLOR
                                           NEPHTYS CORNUTA  FRANCISCANA
                                           GLYCERA CAPITATA
                                           COMPSOMYAX  SUBDIAPHANA
                                           PRAXILLELLA GRACILIS
                                           PHOLOE MINUTA
                                           PECTINARIA  CALIFORNIENSIS
                                           PARVILUCINA TENUISCULPTA
                                           PLATYNEREIS BICANALICULATA
                                           GLYCERA AMERICANA
                                           AMPHIODIA (AMPHISPINA) URTIC<
(ug/gTOC)


      150+
      120 +
       90 +
       60 +
           +•
           0
             * *
                  FLUORANTHENE vs. CUMULATIVE FREQUENCY
                                                * * *  *
                                         *  * *
                               *****
                          * *
                    * * *
20
40        60        80
 Cumulative Frequency
—+
 100
                               Attachment 8

-------
                              NAPHTHALENE
SLC = 41.394 ug/gTOC [0.0 -
Cumulative SSLC No. of
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Freq. ( ", )
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
(ug/g Org.C)
41.394
41.394
41.394
41.765
41.765
41.765
43.333
43.333
47.436
47.436
47.436
51.980
51.980
51.980
51.980
51.980
51.980
52.055
52.055
52.055
57.059
57.059
57.059
Obser .
20
43
25
24
22
51
25
24
55
51
52
21
26
50
20
49
34
30
21
29
29
27
28
                                           Organism
                                           PRIONOSPIO CIRRIFERA
                                           CAPITELLA CAPITATA
                                           ARMANDIA BREVIS
                                           PRAXZLLELLA GRACILIS
                                           EUCHONE INCOLOR
                                           AXINOPSIDA SERICATA
                                           GONIADA BRUNNEA
                                           COMPSOMYAX SUBDIAPHANA
                                           MACOMA CARLOTTENSIS
                                           GLYCERA CAPITATA
                                           EUPHILOMEDES CARCHARODONTA
                                           SPIOPHANES BERKELEYORUM
                                           SPIOCHAETOPTERUS COSTARUM
                                           PRIONOSPIO STEENSTRUPI
                                           PHYLLODOCE HARTMANAE
                                           NEPHTYS  FERRUGINEA
                                           NEPHTYS  CORNUTA  FRANCISCANA
                                           PLATYNEREIS  BICANALICULATA
                                           PECTINARIA CALIFORNIENSIS
                                           GLYCERA  AMERICANA
                                           PHOLOE MINUTA
                                           PARVILUCINA  TENUISCULPTA
                                           AMPHIODIA (AMPHISPINA) URTICA
(ug/gTOC:
     55.0 +
     50.0 +
     45.0 +
     40.0 +
                  NAPHTHALENE vs.  CUMULATIVE FREQUENCY
               *  •*
                                                         * * *
                                     * * *
                               * * *
                    20
._ +	H	+ .
 40        60         80
 Cumulative  Frequency
100
                               Attachment 9

-------
                   PCS
SLC = 3.656 ug/gTOC [0.0  -  4.583)  a=0.03

Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
Cumulative
Freq. ( ", )
2.0
3.9
5.9
7.8
9.8
11.8
13.7
15.7
17.6
19.6
21.6
23.5
25.5
27.5
29.4
31.4
33.3
35.3
37.3
39.2
41.2
43.1
45.1
47.1
49.0
51.0
52.9
54.9
56.9
58.8
60.8
62.7
64.7
66.7
68.6
70.6
72.5
74.5
76.5
78.4
80.4
82.4
84.3
86.3
88.2
90.2
92.2
94.1
96.1
98.0
100.0
SSLC
(ug/g Org.C)
2.222
3.394
3.871
4.583
4.634
4.714
4.714
4.841
4.841
4.841
4.841
4.841
6.000
6.000
6.000
6.000
7.500
8.000
8.000
8.000
8.000
8.854
10.000
10.000
10.000
10.625
10.625
10.941
11.417
11.731
13.769
16.935
25.000
30.118
33.103
33.905
33.905
34.194
39.683
40.017
41.143
41.143
46.025
46.307
47.817
47.911
49.547
52.058
56.307
58.774
71.315
Mo. of
Obser .
21
21
32
24
22
24
27
30
29
27
20
26
33
23
23
51
33
39
24
22
24
25
27
33
26
29
30
32
24
37
25
55
36
21
35
20
20
54
20
56
28
109
90
20
50
95
64
67
89
42
40
                           Organism
                           SCALIBREGMA  INFLATUM
                           SPIOCHAETOPTERUS COSTARUM
                           NEPHTYS  FERRUGINEA
                           HARMOTHOE  EXTENUATA
                           EUCHONE  ELEGANS
                           DRILONEREIS  LONGA
                           SPIOPHANES BOMBYX
                           EUCHONE  INCOLOR
                           ANOBOTHRUS GRACILIS
                           ARCTICA  ISLANDICA
                           PARAONIS GRACILIS
                           NINOE  NIGRIPES
                           NUCULA PROXIMA
                           COSSURO  LONGOCIRRATA
                           NEPHTYS  INCISA
                           PHOLOE MINUTA
                           THARYX ACUTUS
                           ARICIDEA CATHERINAE
                           UNCIOLA  IRRORATA
                           CAULLERIELLA CF KILLARIENSIS
                           GONIADELLA GRACILIS
                           LUMBRINEREIS HEBES
                           PHERUSA  AFFINIS
                           THARYX ANNULOSUS
                           PHYLLODOCE HUCOSA
                           PITAR  MORRHUANUS
                           LUMBRINEREIS ACICULARUM
                           TELLINA  AGILIS
                           GLYCERA  DIBRANCHIATA
                           AMPHIODIA  (AMPHISPINA)  URTICA
                           HETEROPHOXUS OCULATUS
                           EUPHILOMEDES CARCHARODONTA
                           GONIADA  BRUNNEA
                           AMPELISCA  BREVISIMULATA
                           COMPSOMYAX SUBDIAPHANA
                           AMPHARETE  ARCTICA
                           STHENELANELLA UNIFORMIS
                           MEDIOMASTUS  AMBISETA
                           ARMANDIA BREVIS
                           PECTINARIA CALIFORNIENSIS
                           PRIONOSPIO CIRRIFERA
                           PRIONOSPIO STEENSTRUPI
                           AXINOPSIDA SERICATA
                           CHLOEIA  PINNATA
                           PARAPRIONOSPIO  PINNATA
                           GLYCERA  CAPITATA
                           CAPITELLA  CAPITATA
                           ilACOMA CARLOTTENSIS
                           PARVILUCINA  TENUISCULPTA
                           SPIOPHANES BERKELEYORUM
                           TELLINA  CARPENTERI

-------
                      PCB vs. CUHMIATTVE  FREQUENCY
(ug/gTOC)
       60 +
         I
         I
         I
         I
       40 +
                       *****
                   * ** *
                                             * * * *
       20 +
                         **********
             ************
        0 +
                     20
40        60        80
 Cumulative Frequency
100
                            Attachment  10 (cont.)

-------
            PHENANTHRENE


Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
SLC =
Cumulative
Freq. ( °; )
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
36.775 ug/gTOC [0.0 -
SSLC
(ug/g Org.C)
36.576
37.826
38.356
38.514
39.726
39.726
40.588
40.588
40.588
40.588
40.588
52.294
52.294
52.294
52.294
54.167
55.372
55.372
55.372
75.000
75.000
75.000
75.000
No. of
Obser .
25
20
25
20
27
52
25
51
36
53
25
21
55
52
46
54
22
35
29
26
31
27
29
                          Organism
                         ARMANDIA BREVIS
                         PRIONOSPIO  CIRRIFERA
                         EUCHONE  INCOLOR
                         PHYLLODOCE  HARTMANAE
                         GONIADA  BRUNNEA
                         AXINOPSIDA  SERICATA
                         PRAXILLELLA GRACILIS
                         NEPHTYS  FERRUGINEA
                         NEPHTYS  CORNUTA  FRANCISCANA
                         EUPHILOMEDES CARCHARODONTA
                         COMPSOMYAX  SUBDIAPHANA
                         PECTINARIA  CALIFORNIENSIS
                         MACOMA CARLOTTENSIS
                         GLYCERA  CAPITATA
                         CAPITELLA  CAPITATA
                         PRIONOSPIO  STEENSTRUPI
                         SPIOPHANES  BERKELEYORUM
                         PHOLOE MINUTA
                         AMPHIODIA  (AMPHISPINA) URTICA
                         SPIOCHAETOPTERUS COSTARUM
                         PLATYNEREIS BICANALICULATA
                         PARVILUCINA TENUISCULPTA
                         GLYCERA  AMERICANA
PHENANTHRENE vs.  CUMULATIVE FREQUENCY
( ug/gTOC ) |
1
72 +
1
1
1
1
60 +
1
1
1 * * *
1
48 +
1
1
1 ****•**•*
I * -v *
36 +
1
0 20 40 60
* * * *
* * * *
80 100
             Cumulative Frequency
             Attachment 11

-------
                                 PYRENE
               SLC = 66.482 ug/gTOC  [0.0  -  75.00)  a=0.026
Cumulative
Rank
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Frea. ( % )
4.3
8.7
13.0
17.4
21.7
26.1
30.4
34.8
39.1
43.5
47.8
52.2
56.5
60.9
65.2
69.6
73.9
78.3
82.6
87.0
91.3
95.7
100.0
SSLC
(ug/g Org.C)
65.217
73.171
74.380
75.000
82.375
82.375
84.932
87.671
87.671
87.671
94.118
94.118
94.118
100.000
100.000
100.000
100.000
100.000
105.882
105.882
105.882
105.882
123.529
No. of
Obser .
20
25
22
27
26
20
52
51
55
53
58
52
48
25
21
36
28
25
31
27
29
29
39
                                            Organism
                                           PRIONOSPIO CIRRIFERA
                                           ARMANDIA BREVIS
                                           SPIOPHANES BERKELEYORUM
                                           GONIAOA BRUNNEA
                                           SPIOCHAETOPTERUS COSTARUM
                                           PHYLLODOCE HARTMANAE
                                           AXINOPSIDA SERICATA
                                           NEPHTYS FERRUGINEA
                                           MACOMA CARLOTTENSIS
                                           EUPHILOMEDES CARCHARODONTA
                                           PRIONOSPIO STEENSTRUPI
                                           GLYCERA CAPITATA
                                           CAPITELLA CAPITATA
                                           PRAXILLELLA GRACILIS
                                           PECTINARIA CALIFORNIENSIS
                                           MEPHTYS CORNUTA FRANCISCANA
                                           EUCHONE INCOLOR
                                           COMPSOMYAX SUBDIAPFANA
                                           PLATYNEREIS BICANALICULATA
                                           PARVILUCINA TENUISCULPTA
                                           GLYCERA AMERICANA
                                           AMPHIODIA  (AMPHISPINA) URTICA
                                           PHOLOE MINUTA
(ug/gTOC)

      120 +
      100 +
       80 +
       60 +
         I
                     PYRENE vs. CUMULATIVE FREQUENCY
                                   * * *
                               * *
                      * * *
                  * *
                                                    *   *  *  *
           *  * * * *
                    20
-j.	+	+ .
••10        60        80
 Cumulative Frequency
—+
 100
                               Attachment 12

-------