FINAL REPORT RECALCULATION OF SCREENING LEVEL CONCENTRATIONS FOR NONPOLAR ORGANIC CONTAMINANTS IN MARINE SEDIMENTS Prepared by Jerry M. Neff, Jack Q. Word, and Thomas C. Gulbransen December 1987 Prepared for U.S. Environmental Protection Agency Criteria and Standards Division Office of Water Regulation and Standards Washington, D.C. Submitted by BATTELLE Washington Environmental Program Office 2030 M Street, N.W. Washington, D.C. ------- INTRODUCTION In Work Assignment 56, Task IV, Battelle evaluated the screening level concentration (SLC) approach to developing sediment quality criteria for nonpolar organic contaminants in freshwater and marine sediments. The results of that effort were summarized in a final report titled, Sediment Quality Criteria Methodology Validation: Calculation of Screening Level Concentrations from Field Data. The report was submitted to EPA in July 1986. In that report, Battelle identified a lack of standardization of taxonomic nomenclature and identifications of benthic infauna among the several large field databases used for calculating marine SLCs. Therefore, it was decided to review the marine databases to identify synonymies and inappropriate taxonomic distinctions, and to eliminate data sets in which the taxonomy was suspected of being inaccurate. This work was performed as a task in Work Assignment 77. The objectives of this task were to o Edit the database used to calculate SLC values for marine sediments o Remove any questionable data sets from the SLC database o Based on the revised data base, recalculate SLCs for selected nonpolar organic contaminants in marine sediments. MATERIALS AND METHODS The species lists from the data sets for southern California and Puget Sound were reviewed. Species names that were synonymous were combined. In addition, the reasonableness and rigor of the overall taxonomic identifications were reviewed on the basis of knowledge of the taxonomy of the benthic fauna of the study areas. Inappropriate taxonomic distinctions were identified and corrected. ------- Any suspect species lists were noted and the data sets containing them were eliminated from the database. Species that were identified as synonymous were combined, inappropriate taxonomic distinctions were corrected, and this information was entered into the database. In addition, the two southern California data sets were reviewed and all species that occurred in both data sets were combined if the resulting combination produced the minimum of 20 observations required for calculating a species screening level concentration (SSLC). The revised database was then used to calculate SSLCs, SLCs, and the 95 percent confidence intervals for the SLCs for 12 nonpolar organic contaminants in marine sediments. The methods used were those described in detail in the 1986 final report (Neff et al., 1986). The compounds were total DDT, total polychlorinated biphenyls (PCBs), and 10 polycyclic aromatic hydrocarbons (acenaphthalene, naphthalene, fluorene, phenanthrene, anthracene, -fluoranthene, benz(a)anthracene, chrysene, pyrene, and benzo(a)pyrene). RESULTS Several synonymies or inappropriate taxonomic distinctions were identified in the data sets. The corrected species were combined under the currently accepted taxonomic designation. The following synonymies or inappropriate taxonomic distinctions were identified and corrected: o Ampharete. arctica, A. goesi, A. labrops, A. acutifrons o Goniada brunnea, G. maculata o Mediomastus ambiseta, M. spp., M. californierisis o Prionospio cirriferra, Minuspio cirriferra. ------- Serious technical problems were identified in the taxonomy of the Puget Sound "Eight Bay Study." Therefore, this data set was deleted from the database. This resulted in loss of some data for 20 species and loss of some SSLC values for all contaminants except DDT, pyrene, and benzo(a)pyrene. By combining species that were present in the two southern California data sets, it was possible to calculate SSLC values for five additional species. These SSLCs then were added to those already in the database and were used in the recalculation of SLCs. The SLC values derived from the revised database are summarized in Table 1. The SSLC values and resulting frequency distribution curves are presented in Attachments 1 through 12. In all but one case, the SLC values calculated with the revised database were higher than the values calculated with the old database.fThe exception was P.CBs. The SLC value for PCBs ;* +£' dropped slightly from 4.26 tor 3.66 j/g/g organic carbon'. None of the changes should be considered statistically significant, given the relatively large 95 percent confidence intervals for most values. Revision of the database allowed us to calculate SLCs for three compounds for which SLCs were not calculated as part of the original work assignment. These were acenaphthalene, fluorene, and anthracene. SLCs for these compounds were in the range of 4.74 to 16.3 vg/g organic carbon. DISCUSSION Quality control review of the data sets used to calculate SLCs resulted in the combination of several entries for synonymous or misidentified species in the species lists, addition of species occurrences for species that occurred in two data sets, and elimination of one questionable data set. These changes in the database resulted in small changes in the calculated SLC values for several nonpolar organic contaminants ------- TABLE 1. OLD AND RECALCULATED SCREENING LEVEL CONCENTRATIONS FOR NONPOLAR ORGANIC COMPOUNDS IN MARINE SEDIMENTS (ALL VALUES ARE pg/g ORGANIC CARBON) Compound DDT PCB Acenaphthalene Naphthalene Fluorene Phenanthrene Anthracene Fluoranthene Benz( a) anthracene Chrysene Pyrene Benzol a)pyrene Screening Old Value 42.8 4.26 — 36.7 — 25.9 — 43.2 26.1 38.4 43.4 39.6 Level Concentration New value 50.5 3.66 4.74 41.4 10.1 36.8 16.3 64.4 26.1 38.4 66.5 39.7 ------- in marine sediments. There was no change for three compounds, a decrease in the SLC for one compound, and an increase in the SLC for five compounds. None of the changes were sufficiently large that the new value was outside the 95 percent confidence interval for the old value. Therefore, the differences were considered statistically insignificant. The largest change was an approximately 50 percent increase in the SLC for pyrene from 43.4 to 66.5 j/g/g organic carbon. Most of the changes were between 10 and 15 percent. These results indicate that the SLC approach is not particularly sensitive to minor changes in the database from which SLCs are calculated. As the number of observations » increases and the number of SSLCs used to calculate SLCs grows, the sensitivity of the calculated SLC values to small changes in the database will decrease further. These results confirm that the SLC approach to sediment criteria development or validation has basic technical validity. Its strengths include the use of real field data on the cooccurence of contaminants and infaunal animals in sediments and the lack of any a priori assumptions about the relationship between the distribution of benthic fauna and the concentrations of contaminants in sediments. These attributes of the SLC approach make it an ideal candidate for a method to validate sediment quality criteria developed by more theoretical approaches, such as equilibrium partitioning. The SLC approach should be further developed to improve the statistical rigor of the SLC calculation process and the technical validity of the SLC values generated. Several variations of different aspects of the method for calculating the SLC should be tried and evaluated by sensitivity analysis. As many additional data sets as possible should be added to the database. A large number of additional data sets have been identified and it may be possible to increase the number of stations in the database to about 1000. All data sets should be subjected to rigorous quality assurance review. Entry of the ------- complete database into a sophisticated computer database management system will facilitate combination of species lists from different data sets, entry of new data sets as they become available, and performance of sensitivity and statistical analyses on the SLC calculation method and the resulting SLC values. It is essential that EPA has an independent method to validate the EP approach or other approach for derivation of sediment quality criteria. The SLC approach is the ideal one for that purpose. LITERATURE CITED Neff, J.M., O.J. Bean, B.W. Cornaby, R.M. Vaga, T.C. Gulbransen, and J.A. Scanlon. 1986. Sediment Quality Criteria Methodology Validation: Calculation of Screening Level Concentrations from Field Data. Report to U.S. Environmental Protection Agency, Criteria and Standards Division, Office of Water Regulation and Standards, Washington D.C. 60 pp. ------- ACENAPHTHALENE Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 SLC = Cumulative Freq. U) 4.8 9.5 14.3 19.0 23.8 28.6 33.3 38.1 42.9 47.6 52.4 57.1 61.9 66.7 71.4 76.2 81.0 85.7 90.5 95.2 100.0 4.739 ug/gTOC SSLC (ug/g Org.C) 4.706 5.479 5.714 5.714 6.522 6.522 6.522 7.190 7.190 7.190 7.190 7.190 7.391 7.391 7.391 9.286 9.286 13.861 13.861 13.861 15.702 [0.0 - No. of Obser . 24 25 21 28 23 41 49 33 53 24 51 25 47 47 49 28 28 26 27 25 . 20 Organism COMPSOMYAX SUBDIAPHANA PRAXILLELLA GRACILIS PECTINARIA CALIFORNIENSIS AMPHIODIA (AMPHISPINA) URTICA EUCHONE INCOLOR CAPITELLA CAPITATA AXINOPSIDA SERICATA NEPHTYS CORNUTA FRANCISCANA HACOMA CARLOTTENSIS GONIAOA BRUNNEA EUPHILOMEDES CARCHARODONTA ARMANDIA BREVIS PRIONOSPIO STEENSTRUPI NEPHTYS FERRUGINEA GLYCERA CAPITATA PLATYNEREIS BICANALICULATA GLYCERA AMERICANA SPIOCHAETOPTERUS COSTARUM PHOLOE MINUTA PARVILUCINA- TENUISCULPTA SPIOPHANES BERKELEYORUM (ug/gTOd 14.0 + 10.5 + 7.0 + 3.5+ +- 0 ACENAPHTHALENE vs. CUMULATIVE FREQUENCY * * * * * * • - + - 20 * * * * * * * * * * * 60 80 Cumulative Frequency —+ 100 Attachment 1 ------- ANTHRACENE SLC = 16.316 ug/gTOC [0.0 Cumulative SSLC No. of Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Freq. ( ', ) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 (ug/g Org.C) 15.953 18.235 19.130 19.595 19.595 19.595 20.000 20.000 20.183 20.183 20.183 21.739 21.739 21.739 21.739 23.288 23.288 23.288 27.273 27.273 27.273 27.273 42.353 Obser. 25 23 20 20 35 25 21 52 26 53 43 25 50 56 52 50 21 30 26 30 27 29 30 - 19.596) a-0.026 Organism ARMANDIA BREVIS EUCHONE INCOLOR PRIONOSPIO CIRRIFERA PHYLLODOCE HARTMANAE NEPHTYS CORNUTA FRANCISCANA COMPSOMYAX SUBDIAPHANA SPIOPHANES BERKELEYORUM AXINOPSIDA SERICATA GONIAOA BRUNNEA EUPHILOMEDES CARCHARODONTA CAPITELLA CAPITATA PRAXILLELLA GRACILIS NEPHTYS FERRUGINEA HACOMA CARLOTTENSIS GLYCERA CAPITATA PRIONOSPIO STEENSTRUPI PECTINARIA CALIFORNIENSIS GLYCERA AMERICANA SPIOCHAETOPTERUS COSTARUM PHOLOE MINUTA PARVILUCINA TENUISCULPTA AMPHIODIA (AMPHISPINA) URTICP PLATYNEREIS BICANALICULATA (ug/gTOC) 40.0 + 32.0 + 24.0 + ANTHRACENE vs. CUMULATIVE FREQUENCY * * * * 16.0+ * I * * * * * * * * * * * * * * * * 20 40 60 80 Cumulative Frequency 100 Attachment 2 ------- BENZ(A)ANTHRACENE SLC = 26.119 ug/gTOC (0.0 - 40 Cumulative Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21- 22 23 Freq. ( °, ) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 SSLC (ug/g Org.C) 24.348 35.477 35.477 40.952 41.322 42.466 44.118 44.118 44.118 44.118 44.118 44.118 47.647 47.647 47.647 47.647 47.647 47.647 47.945 51.765 51.765 51.802 51.802 No. of Obser. 0 20 PR 21 SP 25 AR 26 GO 26 SP 52 AX 56 MA 52 GL 53 EU 28 EU 25 CO 49 CA 57 PF 25 PF 21 PE 27 P* 50 NE 35 NE 29 A* 38 PE 30 GI 30 PI 20 PF a=0.026 Organism PRIONOSPIO CIRRIFERA SPIOPHANES BERKELEYORUM ARMANDIA BREVIS GONIADA BRUNNEA SPIOCHAETOPTERUS COSTARUM AXINOPSIDA SERICATA MACOMA CARLOTTENSIS GLYCERA CAPITATA EUPHILOMEDES CARCHARODONTA EUCHONE INCOLOR COMPSOMYAX SUBDIAPHANA CAPITELLA CAPITATA PRIONOSPIO STEENSTRUPI PRAXILLELLA GRACILIS PECTINARIA CALIFORNIENSIS PARVILUCINA TENUISCULPTA NEPHTYS FERRUGINEA NEPHTYS CORNUTA FRANCISCANA AMPHIODIA (AMPHISPINA) URTICA PHOLOE MINUTA GLYCERA AMERICANA PLATYNEREIS BICANALICULATA PHYLLODOCE HARTMANAE (ug/gTOC) 48.0 + 40.0 + 32.0 + 24.0 + + - 0 BENZ(A)ANTHRACENE vs. CUMULATIVE FREQUENCY * * * * * * * * ***** * * * * * * 20 40 60 80 Cumulative Frequency —+ 100 Attachment 3 ------- BENZO(A)PYRENE SLC = Cumulative Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Freq. ( \) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 55.2 59.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 39.667 ug/gTOC [0.0 - SSLC Mo. of (ug/g Org.C) 39.604 40.000 46.552 46.795 49.315 49.315 50.000 50.000 50.000 50.000 50.000 50.588 52.910 52.910 52.910 55.372 55.372 55.372 55.372 61.644 61^.644 66\667 137.387 Obser . 21 20 49 26 25 28 25 51 52 25 51 34 56 55 25 37 21 26 49 29 29 29 20 Organism SPIOPHANES BERKELEYORUM PRIONOSPIO CIRRIFERA CAPITELLA CAPITATA SPIOCHAETOPTERUS COSTARUM GONIADA BRUNNEA EUCHONE INCOLOR PRAXILLELLA GRACILIS GLYCERA CAPITATA EUPHILOMEDES CARCHARODONTA COMPSOMYAX SUBDIAPHANA AXINOPSIDA SERICATA NEPHTYS CORNUTA FRANCISCANA PRIONOSPIO STEENSTRUPI HACOMA CARL-Q.TTENSIS ARMANDIA B&ELVIS PHOLOE MINU^A PECTINARIA CALIFORNIENSIS PARVILUCINA TENUISCULPTA NEPHTYS FERRUGINEA GLYCERA AMERICANA AMPHIODt^(AMPHISPINA) URTICA PLATYNERE>IS BICANALICULATA PHYLLODOCE HARTMANAE (ug/gTOC) 120 + 90 + 60 + BENZO(A)PYRENE vs. CUMULATIVE FREQUENCY * * ****** **** 1 * * 1 30 + 0 20 '10 60 80 10 Cumulative Frequency Attachment 4 ------- CHRYSENE SLC = 38.395 ug/gTOC [0.0 - 60.847) a=*0.026 Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Cumulative Freq. Co) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 SSLC (ug/g Org.C) 35.652 52.893 57.143 60.847 62.084 62.084 62.084 63.694 63.694 64.706 64.706 64.706 68.966 68.966 69.863 69.863 75.314 75.314 76.471 76.471 76.471 76.471 76.471 No. of Obser . 20 21 26 25 20 48 51 57 28 50 55 52 25 51 25 21 35 25 30 38 26 29 29 Organism PRIONOSPIO CIRRIFERA SPIOPHANES BERKELEYORUH GONIADA BRUNNEA ARMANDIA BREVIS PHYLLODOCE HARTMANAE CAPITELLA CAPITATA AXINOPSIDA SERICATA PRIONOSPIO STEENSTRUPI EUCHONE INCOLOR NEPHTYS FERRUGINEA MACOMA CARLOTTENSIS EUPHILOHEDES CARCHARODONTA SPIOCHAETOPTERUS COSTARUM GLYCERA CAPITATA PRAXILLELLA GRACILIS PECTINARIA CALIFORNIENSIS NEPHTYS CORNUTA FRANCISCANA COMPSOMYAX SUBDIAPHANA PLATYNEREIS BICANALICULATA PHOLOE MINUTA PARVILUCINA TENUISCULPTA GLYCERA AMERICANA AMPHIODIA (AMPHISPINA) URTICA (ug/gTOC) 72 + 60 + 48 + 36 + I +• 0 CHRYSENE vs. CUMULATIVE FREQUENCY * * * * * * * * * * * * * * * * * * * 20 40 60 80 Cumulative Frequency 100 Attachment 5 ------- DDT SLC = 50.488 ug/gTOC [0.0 - 113.684) a=0.02 Cumulative SSLC Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Freq. (\) 4.8 9.5 14.3 19.0 23.8 28.6 33.3 38.1 42.9 47.6 52.4 57.1 61.9 66.7 71.4 76.2 81.0 85.7 90.5 95.2 100.0 (ug/g Org.C) 50.488 50.438 68.696 113.684 137.692 137.692 202.441 207.917 222.389 954.033 1136.319 1146.004 1186.331 1260.058 1392.128 1407.287 1511.990 1816.188 1999.961 2069.586 2069.586 No. of Obser . 21 27 29 21 20 29 25 20 22 62 44 22 79 45 86 61 101 51 44 37 57 Organism AMPELISCA BREVISIMULATA AMPHIODIA (AMPHISPINA) URTICA EUPHILOMEDES CARCHARODONTA HETEROPHOXUS OCULATUS STHENELANELLA UNIFORMIS COMPSOMYAX SUBDIAPHANA GONIADA BRUNNEA CHLOEIA PINNATA GNATHIA CRENULATIFRONS PECTINARIA CALIFORNIENSIS MEDIOMASTUS AMBISETA PRIONOSPIO CIRRIFERA AXINOPSIDA SERICATA PARAPRIONOSPIO PINNATA GLYCERA CAPITATA PRIONOSPIO STEENSTRUPI PARVILUCINA TENUISCULPTA MACOMA CARLOTTENSIS CAPITELLA CAPITATA SPIOPHANES BERKELEYORUM TELLINA CARPENTERI (ug/gTOC) 1800 + 1200 + 600 + 0+ * * +• 0 DDT vs. CUMULATIVE FREQUENCY * * * * * * * * * * 20 40 60 80 Cumulative Frequency 100 Attachment 6 ------- FLUORENE SLC = 10.123 ug/gTOC [0.0 Cumulative SSLC No. of Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Freq. ( 1 ) 4.8 9.5 14.3 19.0 23.8 28.6 33.3 38.1 42.9 47.6 52.4 57.1 61.9 66.7 Tl.4 <&g.2 81.0 85.7 90.5 95.2 100.0 ( ug/g Org.C) 10.092 10.811 12.353 13.223 13.223 13.223 13.223 13.366 13.366 13.366 13.366 13.366 13.529 13.529 13.529 13.529 13.529 18.824 18.824 18.824 18.824 Obser . 24 24 49 21 53 51 42 20 26 49 47 23 25 25 33 49 24 27 28 28 28 - 13.233) a=0.02 Organism GONIADA BRUNNEA ARMANDIA BREVIS AXINOPSIDA SERICATA PECTINARIA CALIFORNIENSIS MACOMA CARLOTTENSIS EUPHILOMEDES CARCHARODONTA CAPITELLA CAPITATA SPIOPHANES BERKELEYORUM SPIOCHAETOPTERUS COSTARUM PRIONOSPIO STEENSTRUPI NEPHTYS FERRUGINEA EUCHONE INCOLOR PRAXILLELLA GRACILIS PARVILUCINA TENUISCULPTA NEPHTYS CORNUTA FRANCISCANA GLYCERA CAPITATA COMPSOMYAX SUBDIAPHANA PLATYNEREIS BICANALICULATA PHOLOE MINUTA GLYCERA AMERICANA AMPHIODIA (AMPHISPINA) URTICA (ug/gTOC-) 17.5 + 15.0 + 12.5 + 10.0+ FLUORENE vs. CUMULATIVE FREQUENCY * * * * * * * * * * * * * * * * 20 40 60 80 Cumulative Frequency —+ 100 Attachment 7 ------- FLUORANTHENE Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 SLC = Cumulative Freq. CO 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 64.434 ug/gTOC [0.0 - SSLC (ug/g Org.C) 64.286 65.217 66.138 81.081 81.651 81.651 111.765 111.765 124.658 124.658 124.658 124.658 124.658 129.412 129.412 129.412 135.294 135.294 135.294 135.294 146.552 164.384 164.384 No. of Obser . 22 20 25 20 26 27 48 52 58 51 55 53 28 36 52 25 25 39 21 27 31 29 29 Organism SPIOPHANES BERKELEYORUH PRIONOSPIO CIRRIFERA ARMANDIA BREVIS PHYLLODOCE HARTMANAE SPIOCHAETOPTERUS COSTARUM GONIADA BRUNNEA CAPITELLA CAPITATA AXINOPSIDA SERICATA PRIONOSPIO STEENSTRUPI NEPHTYS FERRUGINEA MACOMA CARLOTTENSIS EUPHILOMEDES CARCHARODONTA EUCHONE INCOLOR NEPHTYS CORNUTA FRANCISCANA GLYCERA CAPITATA COMPSOMYAX SUBDIAPHANA PRAXILLELLA GRACILIS PHOLOE MINUTA PECTINARIA CALIFORNIENSIS PARVILUCINA TENUISCULPTA PLATYNEREIS BICANALICULATA GLYCERA AMERICANA AMPHIODIA (AMPHISPINA) URTIC< (ug/gTOC) 150+ 120 + 90 + 60 + +• 0 * * FLUORANTHENE vs. CUMULATIVE FREQUENCY * * * * * * * ***** * * * * * 20 40 60 80 Cumulative Frequency —+ 100 Attachment 8 ------- NAPHTHALENE SLC = 41.394 ug/gTOC [0.0 - Cumulative SSLC No. of Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Freq. ( ", ) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 (ug/g Org.C) 41.394 41.394 41.394 41.765 41.765 41.765 43.333 43.333 47.436 47.436 47.436 51.980 51.980 51.980 51.980 51.980 51.980 52.055 52.055 52.055 57.059 57.059 57.059 Obser . 20 43 25 24 22 51 25 24 55 51 52 21 26 50 20 49 34 30 21 29 29 27 28 Organism PRIONOSPIO CIRRIFERA CAPITELLA CAPITATA ARMANDIA BREVIS PRAXZLLELLA GRACILIS EUCHONE INCOLOR AXINOPSIDA SERICATA GONIADA BRUNNEA COMPSOMYAX SUBDIAPHANA MACOMA CARLOTTENSIS GLYCERA CAPITATA EUPHILOMEDES CARCHARODONTA SPIOPHANES BERKELEYORUM SPIOCHAETOPTERUS COSTARUM PRIONOSPIO STEENSTRUPI PHYLLODOCE HARTMANAE NEPHTYS FERRUGINEA NEPHTYS CORNUTA FRANCISCANA PLATYNEREIS BICANALICULATA PECTINARIA CALIFORNIENSIS GLYCERA AMERICANA PHOLOE MINUTA PARVILUCINA TENUISCULPTA AMPHIODIA (AMPHISPINA) URTICA (ug/gTOC: 55.0 + 50.0 + 45.0 + 40.0 + NAPHTHALENE vs. CUMULATIVE FREQUENCY * •* * * * * * * * * * 20 ._ + H + . 40 60 80 Cumulative Frequency 100 Attachment 9 ------- PCS SLC = 3.656 ug/gTOC [0.0 - 4.583) a=0.03 Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Cumulative Freq. ( ", ) 2.0 3.9 5.9 7.8 9.8 11.8 13.7 15.7 17.6 19.6 21.6 23.5 25.5 27.5 29.4 31.4 33.3 35.3 37.3 39.2 41.2 43.1 45.1 47.1 49.0 51.0 52.9 54.9 56.9 58.8 60.8 62.7 64.7 66.7 68.6 70.6 72.5 74.5 76.5 78.4 80.4 82.4 84.3 86.3 88.2 90.2 92.2 94.1 96.1 98.0 100.0 SSLC (ug/g Org.C) 2.222 3.394 3.871 4.583 4.634 4.714 4.714 4.841 4.841 4.841 4.841 4.841 6.000 6.000 6.000 6.000 7.500 8.000 8.000 8.000 8.000 8.854 10.000 10.000 10.000 10.625 10.625 10.941 11.417 11.731 13.769 16.935 25.000 30.118 33.103 33.905 33.905 34.194 39.683 40.017 41.143 41.143 46.025 46.307 47.817 47.911 49.547 52.058 56.307 58.774 71.315 Mo. of Obser . 21 21 32 24 22 24 27 30 29 27 20 26 33 23 23 51 33 39 24 22 24 25 27 33 26 29 30 32 24 37 25 55 36 21 35 20 20 54 20 56 28 109 90 20 50 95 64 67 89 42 40 Organism SCALIBREGMA INFLATUM SPIOCHAETOPTERUS COSTARUM NEPHTYS FERRUGINEA HARMOTHOE EXTENUATA EUCHONE ELEGANS DRILONEREIS LONGA SPIOPHANES BOMBYX EUCHONE INCOLOR ANOBOTHRUS GRACILIS ARCTICA ISLANDICA PARAONIS GRACILIS NINOE NIGRIPES NUCULA PROXIMA COSSURO LONGOCIRRATA NEPHTYS INCISA PHOLOE MINUTA THARYX ACUTUS ARICIDEA CATHERINAE UNCIOLA IRRORATA CAULLERIELLA CF KILLARIENSIS GONIADELLA GRACILIS LUMBRINEREIS HEBES PHERUSA AFFINIS THARYX ANNULOSUS PHYLLODOCE HUCOSA PITAR MORRHUANUS LUMBRINEREIS ACICULARUM TELLINA AGILIS GLYCERA DIBRANCHIATA AMPHIODIA (AMPHISPINA) URTICA HETEROPHOXUS OCULATUS EUPHILOMEDES CARCHARODONTA GONIADA BRUNNEA AMPELISCA BREVISIMULATA COMPSOMYAX SUBDIAPHANA AMPHARETE ARCTICA STHENELANELLA UNIFORMIS MEDIOMASTUS AMBISETA ARMANDIA BREVIS PECTINARIA CALIFORNIENSIS PRIONOSPIO CIRRIFERA PRIONOSPIO STEENSTRUPI AXINOPSIDA SERICATA CHLOEIA PINNATA PARAPRIONOSPIO PINNATA GLYCERA CAPITATA CAPITELLA CAPITATA ilACOMA CARLOTTENSIS PARVILUCINA TENUISCULPTA SPIOPHANES BERKELEYORUM TELLINA CARPENTERI ------- PCB vs. CUHMIATTVE FREQUENCY (ug/gTOC) 60 + I I I I 40 + ***** * ** * * * * * 20 + ********** ************ 0 + 20 40 60 80 Cumulative Frequency 100 Attachment 10 (cont.) ------- PHENANTHRENE Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 SLC = Cumulative Freq. ( °; ) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 36.775 ug/gTOC [0.0 - SSLC (ug/g Org.C) 36.576 37.826 38.356 38.514 39.726 39.726 40.588 40.588 40.588 40.588 40.588 52.294 52.294 52.294 52.294 54.167 55.372 55.372 55.372 75.000 75.000 75.000 75.000 No. of Obser . 25 20 25 20 27 52 25 51 36 53 25 21 55 52 46 54 22 35 29 26 31 27 29 Organism ARMANDIA BREVIS PRIONOSPIO CIRRIFERA EUCHONE INCOLOR PHYLLODOCE HARTMANAE GONIADA BRUNNEA AXINOPSIDA SERICATA PRAXILLELLA GRACILIS NEPHTYS FERRUGINEA NEPHTYS CORNUTA FRANCISCANA EUPHILOMEDES CARCHARODONTA COMPSOMYAX SUBDIAPHANA PECTINARIA CALIFORNIENSIS MACOMA CARLOTTENSIS GLYCERA CAPITATA CAPITELLA CAPITATA PRIONOSPIO STEENSTRUPI SPIOPHANES BERKELEYORUM PHOLOE MINUTA AMPHIODIA (AMPHISPINA) URTICA SPIOCHAETOPTERUS COSTARUM PLATYNEREIS BICANALICULATA PARVILUCINA TENUISCULPTA GLYCERA AMERICANA PHENANTHRENE vs. CUMULATIVE FREQUENCY ( ug/gTOC ) | 1 72 + 1 1 1 1 60 + 1 1 1 * * * 1 48 + 1 1 1 ****•**•* I * -v * 36 + 1 0 20 40 60 * * * * * * * * 80 100 Cumulative Frequency Attachment 11 ------- PYRENE SLC = 66.482 ug/gTOC [0.0 - 75.00) a=0.026 Cumulative Rank 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Frea. ( % ) 4.3 8.7 13.0 17.4 21.7 26.1 30.4 34.8 39.1 43.5 47.8 52.2 56.5 60.9 65.2 69.6 73.9 78.3 82.6 87.0 91.3 95.7 100.0 SSLC (ug/g Org.C) 65.217 73.171 74.380 75.000 82.375 82.375 84.932 87.671 87.671 87.671 94.118 94.118 94.118 100.000 100.000 100.000 100.000 100.000 105.882 105.882 105.882 105.882 123.529 No. of Obser . 20 25 22 27 26 20 52 51 55 53 58 52 48 25 21 36 28 25 31 27 29 29 39 Organism PRIONOSPIO CIRRIFERA ARMANDIA BREVIS SPIOPHANES BERKELEYORUM GONIAOA BRUNNEA SPIOCHAETOPTERUS COSTARUM PHYLLODOCE HARTMANAE AXINOPSIDA SERICATA NEPHTYS FERRUGINEA MACOMA CARLOTTENSIS EUPHILOMEDES CARCHARODONTA PRIONOSPIO STEENSTRUPI GLYCERA CAPITATA CAPITELLA CAPITATA PRAXILLELLA GRACILIS PECTINARIA CALIFORNIENSIS MEPHTYS CORNUTA FRANCISCANA EUCHONE INCOLOR COMPSOMYAX SUBDIAPFANA PLATYNEREIS BICANALICULATA PARVILUCINA TENUISCULPTA GLYCERA AMERICANA AMPHIODIA (AMPHISPINA) URTICA PHOLOE MINUTA (ug/gTOC) 120 + 100 + 80 + 60 + I PYRENE vs. CUMULATIVE FREQUENCY * * * * * * * * * * * * * * * * * * * 20 -j. + + . ••10 60 80 Cumulative Frequency —+ 100 Attachment 12 ------- |