Bioremediation of Arsenic, Chromium,
Lead, and Mercury
August 2004
Prepared by
Adebowale Adeniji
National Network of Environmental Management Studies Fellow
for
U.S. Environmental Protection Agency
Office of Solid Waste and Emergency Response
Technology Innovation Office
Washington, DC
www.clu-in.org
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
NOTICE
This document was prepared by Adebowale Adeniji, a National Network of Environmental
Management studies grantee, under a fellowship from the U.S. Environmental Protection
Agency This report was not subject to EPA peer review or technical review. The EPA makes no
warranties, expressed or implied, including without limitation, warranty for completeness,
accuracy, or usefulness of the information, warranties as to the merchantability, or fitness for a
particular purpose Moreover, the listing of any technology, corporation, company, person, or
facility in this report does not constitute endorsement, approval, or recommendation by the EPA
This report provides a basic orientation and current status of bioremediation for contaminants
located in the subsurface. This report contains information gathered from a range of currently
available sources, including project documents, reports, periodicals, Internet searches, and
personal communication with involved parties References for each case study are provided
immediately following the case study All sources are organized in alphabetical order at the end
of the document. No attempts were made to independently confirm the resources used. It has
been reproduced to help provide federal agencies, states, consulting engineering firms, private
industries, and technology developers with information on the current status of this project.
This paper addresses the status of the application of biological treatment to clean up hazardous
metals from the earth's subsurface (i e., in situ bioremediation) The target audience includes
federal and state regulators, planners, and site managers The report is available on the Internet at
www clu-in.org/studentpapers/
About the National Network for Environmental Management Studies
The National Network for Environmental Management Studies (NNEMS) is a comprehensive
fellowship program managed by the EPA's Office of Environmental Education The purpose of
the NNEMS Program is to provide students with practical research opportunities and
expenences
Each participating headquarters or regional office develops and sponsors projects for student
research The projects are narrow in scope to allow the student to complete the research by
working full-time during the summer or part-time during the school year Research fellowships
are available in environmental policy, regulations, and law; environmental management and
administration, environmental science, public relations and communications, and computer
programming and development
NNEMS fellows receive a stipend at a level determined by the student's level of education, the
duration of the research project, and the location of the research project Fellowships are offered
to undergraduate and graduate students Students must meet certain eligibility criteria
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
CONTENTS
Introduction 5
Chemical Process of Bioremediation 7
Mobility of Metal Contaminants 8
Superfund, DoD, and DOE Sites 8
Mechanisms for Metal Remediation 12
Immobilization 12
Mobilization 13
Arsenic 14
Health and Toxicology . 14
Chemical and Physical Characteristics 15
Case Study: Mobilization of Arsenite by Dissimilatory Reduction of Absorbed Arsenate
(Zobrist, 2000) 15
Case Study A New Chemolithoautotrophic Arsemte-Oxidizing Bacterium Isolated from a
Gold Mine. Phylogenetic, Physiological, and Preliminary Biochemical Studies (Santini, Sly,
Schnagl, and Macy, 2000) 16
Summary 18
References 19
Chromium 20
Health and Toxicology 20
Chemical and Physical Charactenstics 20
Case Study: Reduction and Precipitation of Chromate by Mixed Culture Sulphate-Reducing
Bacterial Biofilms 21
Case Study. Bioremediation of chromium with Cheese Whey (Rynk, 2004) 22
Summary 23
Further Remediation Techniques for Chromium 24
References 24
Lead 26
Health and Toxicology 26
Chemical and Physical Characteristics 27
Subsurface Mobility and Immobility 27
Groundwater Bioremediation 28
Case Study "Dynamics of Lead Immobilization in Sulfate Reducing Biofilms" 28
(Beyenal and Lewandowski, 2004) 28
Case Study Metal Bioremediation Through Growing Cells (Malik, 2003) 30
Summary 32
References 33
Mercury 34
Health and Toxicology 34
Chemical and Physical Characteristics 34
Groundwater Treatment 35
Field Scale: Removal of Mercury from Chemical Wastewater by Microorganism in Technical
Scale (Wagner-Dobler, Von Canstem, Li, Timmis, and Deckwer, 2000) 35
Case Study: Microbial Reduction and Oxidation of Mercury in Freshwater (Siciliano,
O'Driscoll, and Lean, 2002) 37
Summary 38
References 39
3
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Bibliography And Additional Reading 41
Personal Contacts 43
FIGURES
Figure 1 9
Figure 2 . .... 10
Figures 11
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
INTRODUCTION
Bioremediation technology uses microorganisms to reduce, eliminate, contain, or transform to
benign products contaminants present in soils, sediments, water, and air Evidence of kitchen
middens (ancient household garbage dumps) and compost piles dates back to 6000 B C. (NABIR
Pnmer, 2003), demonstrating that some form of bioremediation was practiced by humans since
the beginning of recorded history. Bioremediation was used over 100 years ago with the opening
of the first biological sewage treatment plant in Sussex, UK, in 1891 (NABIR Pnmer, 2003)
However, the word "bioremediation" is fairly new; first appearing in a peer-reviewed scientific
literature in 1987 (NABIR Pnmer, 2003)
Interest in the application of bioremediation for environmental protection efforts in the United
States has increased within the last decade While it has been known that bioremediation can be a
cost efficient and reliable method for removing hazardous waste from sites heavily contaminated
sites with organic compounds, in the last decade researchers have also discovered that
bioremediation technology can be used at sites contaminated with solvents, explosives,
polycychc aromatic hydrocarbons (PAHs), and polychlormated biphenyls (PCBs) (NABIR
Pnmer, 2003) Even more recently, researchers have discovered that microbial processes are
beginning to be used to cleanup radioactive and metallic contaminants—two of the most
common and most recalcitrant components of hazardous waste sites (NABIR Pnmer, 2003)
Metal contaminants are commonly found in soils, sediments, and water. Metal pollutants can be
produced through industrial processes such as mining, refining, and electroplating A key factor
to the remediation of metals is that metals are non-biodegradable, but can be transformed
through sorption, methylation, and complexation, and changes in valence state. These
transformations affect the mobility and bioavailability of metals At low concentrations, metals
can serve as important components in life processes, often serving important functions in enzyme
productivity However, above certain threshold concentrations, metals can become toxic to many
species Fortunately, microorganisms can affect the reactivity and mobility of metals.
Microorganisms that affect the reactivity and mobility of metals can be used to detoxify some
metals and prevent further metal contamination.
Staphylococcus, Bacillus, Pseudomonas, Citrobacteia, Klebsilla, and Rhodococcus are
organisms that are commonly used in bioremediation mechanisms (Connor, Landm, Mellor,
O'Donovan, a. 1994 b. 1996) These mechanisms include bioaugmentation, in which microbes
and nutrients are added to the contaminated site, and biostimulation, in which nutnents and
enzymes are added to supplement the intnnsic microbes of the site (Connor, Landin, Mellor,
O'Donovan, a 1994 b. 1996) These organisms are often used in the bioremediation of cadmium
(Connor, Landin, Mellor, O'Donovan, a. 1994 b 1996). Alcaligenes and Pseudomonas have
been used in the bioremediation of chromium (Connor, Landin, Mellor, O'Donovan, a. 1994 b.
1996) Likewise, organisms like Escherichia and Pseudomonas have been used in the
bioremediation of copper (Connor, Landin, Mellor, O'Donovan, a. 1994 b 1996) Currently,
more research is being performed on the use of microbes to degrade metals
It is anticipated that federal, state, and local governments and private industries will annually
invest billions of dollars over the next several decades to clean up sites contaminated with
hazardous waste (NABIR Primer, 2003). This investment validates the need to research the
utilization of microbial processes to clean up contaminated sites.
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Superfund is a federal program managed by the EPA that enforces the Comprehensive
Environmental Response, Compensation, and Liability Act (CERCLA) created for site
identification and cleanup (USEPA Office of Solid Waste and Emergency Response, 2004)
Volatile organic compounds (VOCs) and metals are the major contaminants found at Superfund
sites (USEPA Office of Solid Waste and Emergency Response, 2004) As of May 2003, there
were 456 federal and non-federal Superfund sites listed on the National Pnonties List (NPL) that
still required at least one more additional remedial action (USEPA Office of Solid Waste and
Emergency Response, 2004) EPA reports that 77% of these sites need to be remediated for
metals, 78% for volatile organic compounds, and 71% for semivolatile organic compounds
(SVOCs). A 2001 study from the EPA predicts that within the next 10 years, an additional 23 to
49 sites will need to undergo extreme cleanup (USEPA, 2004).
Metal contaminants are not just at Superfund sites. Sites managed by the Department of Defense
(DoD) and the Department of Energy (DOE) are also contaminated with metals. The DoD is
responsible for cleaning up contamination at DoD facilities where numerous industrial,
commercial, training, and weapons testing activities took place (USEPA, 2004). The DoD
reports that approximately 9,000 of its sites require remediation (USEPA, 2004). The most
prevalent groundwater contaminant groups are volatile organic contaminants and metals, which
appear at 74 percent and 63 percent of DoD sites (USEPA, 2004) This further supports the need
for research on the bioremediation of metals. Metals were found between 63-79 percent of the
DOE sites (USEPA, 2004).
The DOE's sites contain radiation hazards, volumes of contaminated soil, and nuclear waste
(USEPA, 2004) These sites account for one third of the total U.S. cleanup market (USEPA
2004). Metals, such as lead, chromium VI, mercury, zinc, beryllium, arsenic, cadmium, and
copper are abundant throughout these sites (USEPA, 2004) Thus, it goes without saying that the
bioremediation of metal contaminants can have a wide application to site cleanup
These metal contaminants pose adverse health effects to those who live near these polluted sites
Metal waste is commonly found in soil, sediments, and water. Breathing, eating, drinking, and
skin contact are all possible exposure routes for metal contaminants Metals such as mercury,
lead, and arsenic, potentially can be toxic to the kidneys, decrease mental capabilities,-and cause
weakness, headaches, abdominal cramps, diarrhea, and anemia (USEPA, 2004) Chronic
exposure to these pollutants can cause permanent kidney and brain damage (USEPA, 2004)
In situ bioremediation is a technique that can be used to reduce the spread of metal contaminants
by applying biological treatment to hazardous chemicals in soil and groundwater (Connor,
Landin, Mellor, O'Donovan, a. 1994 b.1996). In situ bioremediation has the ability to transform
contaminants to less toxic compounds, making this a promising environmental cleanup technique
(National Research Council, 1993) It accelerates contaminant desorption and dissolution by
treating pollutants close to their source. Methods such as pump-and-treat only remove or destroy
contaminants in groundwater, but not those contaminants sorbed in soil or solids in the aquifer
(National Research Council, 1993)
Although the use of bioremediation is rapidly growing, in situ bioremediation of metals is not
widely understood (National Research Council, 1993). The many intricacies of microbial
metabolism related to metals have not been grasped fully because successful usage of microbes
to clean ip metals is a complex matter. Furthermore, the process of verifying whether or not
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Bioremediation off Arsenic, Chromium, Lead, and Mercury
complete bioremediation has actually occurred (meaning the metal contaminant changed forms
via abiotic chemical reactions) is an issue researchers are still struggling with in the labs.
Demonstrating that lab-grown microbes have the potential to degrade the contaminant is not
enough to sufficiently prove that microorganisms can completely cleanup a site (National
Research Council, 1993). Moreover, there is still a need for researchers to verify that the
bioremediation of metal contaminants is a permanent process.
Chemical Process of Bioremediation
Bioremediation involves the use of microorganisms to aid with the destruction of contaminants, a
process called microbial metabolism This process involves biochemical reactions or pathways in
an organism that result in activity, growth, and reproduction of that organism Chemical
processes involved in microbial metabolism consist of reactants, contaminants, oxygen, or other
electron acceptors, which convert metabolites to well-defined products The microbial
metabolism system enables organisms to retrieve carbon, electrons, and other vital components
to survive. In some cases, the contaminant may be transformed while the microorganisms seek
other sources of energy or carbon This reaction is described as "cometabolism," during which
the transformation of the contaminant yields little or no benefit to the cell Secondary utilization
is another way to describe a non-beneficial biotrans formation This transformation of the
contaminant is an incidental reaction catalyzed by enzymes present in the cell's metabolic
system (National Research Council, 1993)
Aerobic respiration is a process that involves microorganisms using oxygen to oxidize a carbon
source that may exist in a contaminant. Many microorganisms use aerobic respiration as a way to
destroy organic contaminants Under aerobic conditions, a drop in oxygen concentration occurs
when microbes are active, which is instrumental in the reproduction of some living organisms.
During this process, oxygen is reduced resulting in the formation of water
Microorganisms that can live without oxygen use anaerobic respiration for a metabolic process.
Unlike aerobic respiration, where oxygen serves as a main electron acceptor, anaerobic
respiration uses inorganic compounds—such as nitrate, sulfate, and iron—as electron acceptors.
Inorganic molecules, such as ammonium, nitrite, and reduced iron, can serve as electron donors
Once these molecules are accepted as electron donors, they are oxidized and their electrons are
transferred to electron acceptors (usually to oxygen) to produce energy for cell synthesis.
Microorganisms whose primary electron donor is an inorganic molecule must receive their
carbon source from carbon dioxide—a process called carbon dioxide fixation Nitrogen gas,
hydrogen sulfide, and reduced forms of metals are other byproducts of anaerobic respiration In
circumstances where metals are used as electron acceptors by anaerobic organisms, the metal
precipitates, which decreases its concentration and mobility in groundwater (National Research
Council, 1993) In general under anaerobic conditions, concentrations of electron acceptors (such
as nitrate and sulfate) will decrease Today research is focusing on metal contaminants that
convert into a precipitate form when exposed to microorganisms. Metals that undergo this
procedure offer promising solutions concerning the removal of persistent metal contaminants in
the environment
Fermentation is another type of metabolism mechanism that assists with the conversion of a
contaminant into a less harmful compound Fermentation is a metabolic method that occurs
without oxygen through a series of internal electron transfers catalyzed by the microorganisms.
Fermentation products, like acetate, propionate, ethanol, and hydrogen, are examples of elements
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
that can be biodegraded by bacteria (National Research Council, 1993). Some bacteria species
can convert these products to carbon dioxide, methane, and water This conversion can assist
with the cleanup of some contaminant sites.
Another method of microbial metabolism is reductive dehalogenation Reductive dehalogenation
is potentially important in the detoxification of halogenated organic products (National Research
Council, 1993) This method involves microbes catalyzing a halogen atom on the contaminant
and replacing it with a hydrogen atom. This reaction adds two electrons to the contaminant
molecule allowing the molecule to convert into a reduced form (National Research Council,
1993) In order for this method to occur, an electron donor must be taken from a different source
other than a halogenated contaminant. Lactate, acetate, methanol, and other low molecular
weight organic compounds may serve as possible electron donors. Reductive dehalogenation is
not commonly known to generate energy for microorganisms, but it provides a way to eliminate
toxic material from the microorganism's cell (National Research Council, 1993).
Mobility of Metal Contaminants
As stated above, microbes can convert contaminants to less harmful products. However, in
addition to their conversion ability, they can cause contaminants to be demobilized (National
Research Council, 1993) The immobility of metals is primarily caused by reactions that cause
metals to precipitate or chemical reactions that keep metals in a solid phase (Evanko and
Dzombak, 1997). Chemical and physical properties affect the mobility of metals in soils and
groundwater. Under acidic conditions (pH ranges between 4.0-8.5), metal cations are mobile
while anions tend to transform to oxide minerals At high pH levels, cations adsorb into mineral
surfaces and metal anions are mobilized. Hydrous metal oxides of iron, aluminum, and
manganese can affect metal concentrations because these minerals can subtract cations and
anions
"Biocurtam" is a term used to describe the process by which large amounts of biomass stop or
slow contaminant movement. The biomass can then absorb hydrophobic organic molecules
(National Research Council, 1993) A large biomass also can hinder the migration of a
contaminant.
When a microorganism oxidizes or reduces species, this reaction causes metals to precipitate
(National research Council, 1993) Mercury is an example of a metal that can be precipitated
The process begins when mercury (Hg2*) is reduced to mercuric sulfide causing mercury to
transform to a precipitated form (National research Council, 1993) Chromium is another metal
that can convert to a precipitated form with the use of microorganisms The process involves the
reduction of hexavalent chromium (Cr6+) to trivalent chromium (Cr3+), which then can
precipitate to chromium oxides, sulfides, or phosphates (National Research Council, 1993)
Research today is focusing on other metal and radioactive contaminants that can undergo
precipitation processes
Superfund, DoD, and DOE Sites
Today, federal, state, and local governments spend billions of dollars on research efforts to
discover effective and economical ways to remove contaminants from the environment
According to the EPA, metals (excluding radioactive metals) are present at three-quarters of the
nation's Superfund sites (EPA, 2004) EPA estimates that 77,000 known sites still require
remediation and new discoveries of contaminated properties are expected to continue (USEP A,
8
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
2004). It has been estimated that an additional 217,000 additional contaminated sites are likely to
be discovered during the next 30 years. Because of these numbers, extensive research on the
chemical processes of bioremediation and on how metals can be effectively remediated is being
explored.
Superfund Sites
The Superfund Program, administered by EPA, is the federal program to clean up releases at
abandoned or uncontrolled hazardous waste sites. It was established by the Comprehensive
Environmental Response, Compensation, and Liability Act (CERCLA). CERCLA was altered by
the uperfund Amendments and Reauthorization Act of 1986 (SARA) (USEPA 2004). Among
other changes, SARA supports the application of new technology that achieves permanent results
and research to "improve site investigation and cleanup methods."
As of September 2003, EPA had listed 1,244 Superfund sites on the NPL (USEP A, 2004). EPA
reports that 78% of these sites need to be remediated for volatile organics, while 77% need to be
remediated for metals, and 71% for semivolatiles or organics (USEP A, 2004). Of the 12
contaminants most frequently found at Superfund sites, half are metals (primarily arsenic,
chromium, lead, zinc, nickel, and cadmium). Arsenic, chromium, and lead are the most prevalent
metals found at Superfund sites (USEPA, 2004). EPA estimates that at 456 NPL sites with
planned remedial actions, about 66 million cubic yards of soil, sediment, and sludge are
contaminated with metals (USEP A 2004). These data support the extreme need to discover
permanent solutions to remove hazardous materials and metal contaminants from the
environment.
Figure 1 shows the frequencies of the most common contaminants at NPL sites (US EPA 2004).
Conlaminants
V S :?T
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Department of Defense
The Department of Defense (DoD) is comprised of the Air Force, Army, and Navy. This
Department holds the responsibilities for protecting the borders of the United States and also for
cleaning up waste remaining from industrial, commercial, training, and weapons activities. The
DoD estimates that as of September 2003, almost 6,400 contaminated sites were in various
stages of site investigation, and almost 2,700 sites were planning for or in various stages of
ccleanup, bring the total number of sites yet to be cleaned up to more than 9,000. Forty-four
percent of these sites are in six states: Virginia, Texas, Florida, Maryland, Alaska, and California
(USEPA, 2004).
Volatile organic compounds, semivolatile organic compounds, and metals are the most prevalent
types of contaminants at DoD sites (USEPA, 2004). Volatile organic compounds are present at
64% of DoD sites, semivolatile contaminants are present at 57% of these sites, and metals are
present at 72% of these sites (USEPA, 2004). In FY 2004, DoD allocated 20% of its cleanup
budget to evaluate and clean up properties that are to be transferred to other federal, state, or
local government agencies or private parties.
Figure 2 displays the frequency of major contaminant groups (VOCs, metals, SVOCs,
explosives, and other) for the most common DoD site types needing cleanup (USEPA, 2004).
ID vocs
JH Matsls
111 SVOCs
i@ Explosives
i|| Other
Spill Area
Lancfil! Surface Disposal Underground Storage Area
Area Storage Tanks
Site Type
B*«d oft S. 118 sires newJiflfl cleanup fct whiett nsta wwe available aa of September 20 L TOO". M«ethan can appnor &( & efts. Apperwj« Exhibit C-fi show* the nnjahdown o< thn *rfa fef an wto typeo.
DOO, Qfficw of the Deputy Und*i Secretary erf Deteiicft (InolailaiiOfii anc Environment) R«totalton Management Information
System,
Department of Energy
The Department of Energy has more than 10,000 contaminated sites for which it is responsible
for cleanup. More than 5,000 of these have been remediated, leaving about 5,000 sites on 39
DOE installations and other properties. DOE also is responsible for 19 sites currently on the
NPL. The Rocky Flats Environmental Technology site in Colorado, the Idaho National
Engineering Laboratory in Idaho, the Savannah River Site in South Carolina, the Oakridge
Reservation in Tennessee, and the Hanford Reservation in Washington are examples of DOE
installations that contain sites that require remediation (USEPA. 2004).
10
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Radionuclides, metals, and dense nonaqueous phase liquids are the major contaminants
Prevalent metal contaminants include lead, chromium VI, mercury, zinc, beryllium, arsenic,
cadmium, and copper (USEPA, 2004). One main challenge faced by DOE is the lack of
technology available for some of the main contaminants present on the sites, especially
radionuclides. DOE plans to complete active remediation of most of its sites by 2035 (USEPA,
2004). However the monitoring and groundwater treatment programs may continue beyond this
proposed cleanup date (USEPA, 2004)
Figure 3 shows the percentage of contaminated groups that need to be remediated in NPL,
RCRA, DOD, and DOE sites (USEPA, 2004). Metals are present a many hazardous waste sites.
For example, metals (not including radioactive metals) are present at 77% of NPL sites.
Figure 3. Contaminant Groups to be Remediated
Percent of Sites
Remediation Program
VOCs Metals SVOCs
NPL Sites
RCRA Corrective Action Sites
DOD Sites
DOE Sites
78%
67%
64%
38%
77%
46%
72%
55%
71%
32%
57%
38%
Notes
1 )DOE figures for VOCs and SVOCs are combined 90% of DOE sites contain radioactive elements
2)About 1 9% of DOD sites yet to be investigated and/or cleaned up may contain unexploded ordnance or waste
military munitions
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
MECHANISMS FOR METAL REMEDIATION
Today's industrial world has contaminated our soil, sediment, and water sources with hazardous
materials Metal waste is often a result of industrial activities, such as mining, refining, and
electroplating. Mercury, arsenic, lead, and chromium are often prevalent at highly contaminated
sites. This fact holds significant challenges for industries because these metals are difficult to
remove Therefore researchers and industries are researching metals that undergo methylation,
complexation, or changes in valence state. These are noteworthy processes because they aid with
the mobility and bioavailability of metals (NABIR, 2003). There is a large interest in
microorganisms that can facilitate with the transformation and the removal of the metal
contaminant
Remediation of metals often involves five general approaches' isolation, immobilization,
mobilization, physical separation, and extraction (Evanko and Dzombak, 1997) Immobilization
and mobilization involve bioremediation processes. Industries use a combination of more than
one approach to properly treat metal-contaminated sites. The combination of the approaches can
be cost-effective
Immobilization
Immobilization is a technique used to reduce the mobility of contaminants by altering the
physical or chemical characteristics of the contaminant This remediation approach can utilize
microorganisms to immobilize metal contaminants Most immobilization technologies are
performed in situ or ex situ In situ means the treatment is below the ground Ex situ means the
waste is excavated or pumped above the ground for treatment (USEPA, 2001) Ex situ processes
are effective for contaminants located in large volumes of waste
The immobilization technique is based on the characteristics of the site In situ stabilization and
solidification is most beneficial for sites with contamination located less than 8-10 feet below the
ground In situ is preferred because it requires less labor and energy than ex situ Immobilization
is usually accomplished by physically restricting contact between the contaminant or by
chemically altering the contaminant (Evanko and Dzombak, 1997) Chemical reagents and
bacterial reagents assist with the immobilization of metal contaminants
Most sites contaminated with metals use the solidification and stabilization approach to
immobilize metals Thirty percent of all soil at Superfund sites uses solidification and
stabilization as a remediation technique Solidification treatment involves mixing or injecting
chemical agents to the contaminated soil. The prominent mechanism by which metals are
immobilized is by precipitation of hydroxides. The chemical composition of the site, the amount
of water present, and the temperatures are all factors important to the successful use of the
solidification/ stabilization mechanism If these factors are not considered then they can interfere
with the solidification/stabilization process by inhibiting bonding of the waste, decreasing the
stability of the matrix, or reducing the strength of the solidified area Solidification and
stabilization is not an effective solidification mechanism for metal contaminants that exist as
anions, such as (Cr VI) or for metals that have low-solubility hydroxides, such as mercury. This
technique may not be applicable to waste sites containing organic and volatile organics Air
stripping or incineration are more effective ways to remove organics (Evanko and Dzombak,
1997).
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Cement-based binders and stabilizers can be used when implementing the solidification and
stabilization technique. Organic binders are applied to treat metals through polymer
microencapsulation Solidification/stabilization technologies using cement are used in industries
to remediate sites containing metals like chromium, lead, arsenic, mercury, and cadmium
(Evanko and Dzombak, 1997) This method also can be used for sites consisting of arsenic
wastes Bitumen, polyethylene, paraffins, waxes, and other polyolefins are materials used for the
application of organic binders Bitumen asphalt is an inexpensive and common thermoplastic
binder Polymer encapsulation involves organic materials being heated and mixed with the
contaminated matrix at temperatures between 120°C to 200°C . The organic materials polymerize
and solidify the waste and then the waste matrix is encapsulated Polymer encapsulation requires
more energy and more technical equipment than cement based solidification/stabilization
operations (Evanko and Dzombak, 1997).
The stabilization and solidification technique is achieved by mixing the contaminated material
with appropriate amounts of stabilizer material and water The mixture forms a solidified matrix
with the waste. The stabilization and solidification techniques can occur both in situ or ex situ In
situ is preferred for volatile or semi volatile organics. The in situ process is useful for treating
surface or shallow contamination, Deep stabilization/solidification up to 50 ft can be achieved by
using gauged augers (up to 3 ft in diameter each) that can treat 150-400 cubic yards per day
(Evanko and Dzombak, 1997). Ex situ requires excavation, transport, and disposal of hazardous
material However it is more difficult to provide uniform and mixing through the m situ process
because in situ stabilization at some sites requires cohesive soils, oily sands, and clays
Mobilization
Microorganisms can mobilize metals through autotrophic and heterotrophic leaching, chelatoon
by microbial metabolites and siderophores, methylation, and redox transformations
Heterotrophic leaching is when microorganisms can acidify their environment by proton efflux
thus leading to the acidification resulting in the release of free metal cations. Autotrophic
leaching is when acidophilic bacteria retrieve carbon dioxide and obtain energy from the
oxidation of the ferrous iron or reduced sulfate compounds, which causes solubilization of
metals Siderophores are specific Fe (III) ligands and are able to bind to other metals, such as
magnesium, manganese, chromium (III), gallium (III), and radionuclides, such as plutomum
(IV). Methylation involves methyl groups that are enzymatically transferred to a metal, forming
a number of different metalloids. Redox transformations can allow microorganisms to mobilize
metals, metalloids, and organometallic compounds by reduction and oxidation processes There
are various metal-mobilization techniques that can occur (Gadd, 2004) The way a particular
metal becomes mobilized depends on the metal's chemical and physical characteristics
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
ARSENIC
Health and Toxicology
Exposure to arsenic can induce adverse health effects and can occur in a number of ways For
example, primary copper, lead smelters and chemical manufactures release arsenic into the
atmosphere (Klaasenand Watkins III, 2003). Drinking water sources usually contain a few
micrograms of arsenic per liter or less Large amounts of arsenic can be found in seafood
(Klaasen and Watkins III, 2003). Occupational settings also can serve as a source of arsenic
exposure. In fact, many U S. workers at factones that produce pesticides, herbicides and other
agricultural products are exposed to arsenic (Klaasen and Watkins III, 2003). Smeltermg
industries are also sources for high levels of arsenic fumes (Klaasen and Watkins III, 2003)
These industries typically account for the excessive spread of arsenic in the environment.
Arsenic is a metal that can generate multiple adverse health effects because of the many
chemical forms it takes on For example, arsenic can appear in inorganic or organic form.
Arsenic tnoxide, sodium arsenite, and arsenic trichloride are the most common inorganic
tnvalent arsenic compounds (Klaasen and Watkins III, 2003). Neurotoxiciy of both the
peripheral and central nervous systems may be due to inorganic arsenic compounds.
Neurotoxicity usually begins with sensory changes, muscle tenderness, followed by progressive
weakness from the proximal to distal muscle groups (Klaasen and Watkins III, 2003).
Organic forms of arsenic also may be in tnvalent or pentavalent forms and may even occur in
methylated forms as a result of biomethylation in soil, fresh water and sea water in humans
(Klaasen and Watkins III, 2003) Pentavalent inorganic forms of arsenic include arsenic
pentoxide, arsenic acid, and arsenates, such as lead arsenate Pentavalent arsenic forms affect
human enzyme activity (Klaasen and Watkins III, 2003).
Acute illnesses from arsenic exposure consist of fever, anorexia, melanosis, cardiac arrhythmia,
and eventually cardiovascular failure (Klaasen and Watkins III, 2003). A few days after exposure
to arsenic, anemia, and granulocyopenia will appear in the body (Klaasen and Watkins III, 2003)
Exposure to arsenic can inhibit succinic dehydrogenase activity and uncouples oxidative
phosphorylation Uncoupled oxidative phosphorylation is process that results in the stimulation
of mitochondrial ATPase activity (Klaasen and Watkins III, 2003).
Inhibition of mitochondria function also can be caused by arsenic exposure Mitochondria
functions can be inhibited two ways, arsenic can compete with a phosphate during oxidative
phosphorlation and by inhibiting the reduction of NAD (Klaasen and Watkins III, 2003)
Inhibition of mitochondria respiration results in a decrease in cellular production of ATP. The
ingestion of large doses (70-180 mg) of arsenic may cause fatal health problems Chronic
exposure to arsenic produces changes in skin epithelium. One to two weeks of exposure results
in sensory loss in the peripheral nervous system
Tnvalent compounds of arsenic are the most toxic forms of arsenic (Klaasen and Watkins III,
2003). High doses of inorganic arsenic compounds given to a pregnant experimental animal
resulted in various malformations in the fetuses and offspring However, these effects have not
been recognized in humans with excessive occupational exposures to arsenic (Klaasen and
Watkins III, 2003) Studies show a possible association between inhalation exposure to arsenic
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
and skin and lung cancer Some studies indicate that arsenic causes cancer of internal organs
from oral digestion (Klaasen and Watkins III, 2003).
Chemical and Physical Characteristics
Arsenic appears in Group V of the periodic table (ATSDR, 1999) Although it appears in Group
V it is semi metallic, in this document it will be addressed as a metal contaminant. It exists in
four oxidation states -3, 0, +3, +5 (ATSDR, 1999) The elemental form of arsenic is As (0) This
compound appears as yellow and metallic gray (ATSDR, 1999) The metallic gray form is the
stable form of arsenic
In an aerobic environment, As (V) is dominant. Arsenate (As043~) and its various profanation
states, H3 As04, HaAsO^, HAs042", As043" precipitate when metal cations are present (Evanko and
Dzombak, 1997) Metal arsenate complexes are stable under specific conditions Arsenic (As
(V)) possesses the ability to co-precipitate with or absorb into iron oxyhydroxides under acidic
and moderately reducing conditions Co-precipitates are immobile under acidic and moderately
reducing conditions. However, arsenic mobility increases as pH increases (Evanko and
Dzombak, 1997)
Many arsenic compounds absorb strongly to soils and therefore travel short distances in
groundwater and surface water Under reducing conditions, arsenic dominates when it exists as
an arsenite (AsO33") and in its protonated forms, H3As03, HaAsOs", and HAsOs2". Arsemte can
absorb or co-precipitate with metal sulfides and has a high attraction to other sulfur compounds
(Evanko and Dzombak, 1997). Under extreme reducing conditions, elemental arsenic and arsme
AsH3 may be present
Arsenic is often present in its anionic form because it does not from complexes with anions such
as CF and S042" (Evanko and Dzombak, 1997). Biotransformation through methlylation of
arsenic can result in methylated compounds of arsine, such as dimethyl arsme HAs (CHs)2 and
tnmethylarsimc acid (CHj) As02H2 and dimethylarsenic acid (CH3)2 AsOzH (Evanko and
Dzombak, 1997).
Studies suggest absorption and co-precipitation with hydrous iron oxides under most environ-
mental conditions are the most effective removal mechanisms of this complex hazardous
material Under low amounts of reactive metals m soil, arsenates can be leached easily. As (V)
can be mobilized under reducing conditions (Evanko and Dzombak, 1997). Reducing conditions
consisting of alkaline and saline, promote the formation of As (III) with the presence of organic
compounds that form compound units with arsenic (Evanko and Dzombak, 1997).
Case Study: Mobilization of Arsenite by Dissimilatory Reduction of Absorbed
Arsenate (Zobrist, 2000)
This study experiments with cell suspensions of Sulfurospirillum bamessii, a bacterium that
reduces arsenate to arsenite
Introduction
Biological processes aid with the facilitation and mobilization of arsenic This experiment
demonstrates that ferric oxide or oxyhydroxide phases are buried in sediments, and reductive
dissolution of feme oxides can release arsenic and other trace metals into the soil (Zobrist,
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
2000) Solubilization of As (V) occurred when Fe (Ill)-respinng bacterium was incubated with
contaminated lake sediments
Research is discovering anaerobic bacteria species that can achieve respiratory reduction of As
(V) to As (III) (Zobnst, 2000) Respiratory reduction of As (V) to As (III) is called dissirmlatory
reduction and involves two bacteria strains, Sulfurospirilium arsenophilum and Sulfurospmllum
barnesu (Zobnst, 2000). This experiment studies Fe (III) respiring Shewanella alga, an organism
that does not respire As (V), but can release As (V) and Fe (II) into solution (Zobnst, 2000)
Materials and Methods
The preparation of feme and aluminum hydroxides involves the use of a 2-line ferrihydrite that
was synthesized in a polyethylene vessel under a nitrogen (N2) atmosphere (Zobnst, 2000). All
chemicals in this expenment were made with a reagent grade The water in this study was
double-deionized and passed through a Milh-Q-System (Zobnst, 2000) Furthermore, gas from
all the solutions was removed, except for analytical reagents (Zobnst, 2000).
Bacteria
S. bamessu strain was grown in a batch culture with nitrate Nitrate acts as the electron acceptor,
this situation allows the culture cells to reduce As (V) (Zobrist, 2000) Nitrate was chosen as the
electron acceptor in the presence of As (V) or Fe (HI) (Zobrist, 2000) The cells were harvested
by centrifugation and washed twice with a solution consisting of a HEPES buffer at a pH of 7 3
and neutral salts (Zobrist, 2000).
Conclusion
The cell suspensions of S. Bctrnessi were able to reduce arsenate to arsenite. The reduction of Fe
(III) in ferrihydrite to soluble Fe (II) occurred with the cultured cells (Zobrist, 2000). The rate of
arsenate reduction was affected by the method that involved arsenate becoming associated with
the mineral phases and the binding with the arsenate (Zobnst, 2000) The extent of the release of
arsenite was regulated by the absorption of arsenite into the ferrihydnte or alumima phases
(Zobnst, 2000) These results help with understanding the mobilization of arsenic in large
aquifers
Case Study: A New Chemolithoautotrophic Arsenite-Oxidizing Bacterium Isolated
from a Gold Mine: Phylogenetic, Physiological, and Preliminary Biochemical
Studies (Santini, Sly, Schnagl, and Macy, 2000).
Introduction
Arsenic is a compound that is very toxic to many life forms. However, when arsenic is in its
insoluble form, it is not a harmful compound. Arsenic in its insoluble form is found in minerals,
such as arsenopyrite (FeAsS) (Santini, Sly, Schnagl, and Macy, 2000) The oxidation of the
insoluble form of arsenic results in its transformation Chemical and microbial processes often
facilitate this oxidation For instance, microbial processes can convert insoluble arsenic to
arsenite (As III) (HsAsOa), now a soluble form of arsenic (Santini, Sly, Schnagl, and Macy,
2000). Acid mines often possess high concentrations of arsenite The transformation of arsenite
could be further oxidized by other microbial and chemical reactions The oxidation of arsenite
results in the formation of arsenate (As V (H2 As04" + if) Arsenite and arsenate are both very
toxic forms of arsenic. Arsenite is more toxic than arsenate (Santini, Sly, Schnagl, and Macy,
16
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
2000) This information is valuable to the advancement and improvement of bioremediation
techniques.
History shows that arsenite-oxidizing bacteria was first identified in 1918 (Santim, Sly, Schnagl,
and Macy, 2000). Research also shows that more of these organisms have been identified since
1918. Arsenite-oxidizing bacteria are heterotrophic, meaning an organism requires organic
matter for growth. Alcaligenes faecalis is the most common arsenite-oxidizing bacteria.
However, arsenite oxidizing bacteria's heterotrophic condition is considered a detoxification
mechanism rather than a mechanism that supports growth
Pseudomonas arsemtoxidans is the only organism known to gain energy m the presence of
arsenite This organism holds its ability to grow chemolithoautotrophically with arsenite, oxygen,
and carbon dioxide. This expenment reports a new bacterium that was isolated from a gold mine
in the northern territory of Australia that can also grow chemolithoautotrophically with arsenite
In addition, this organism can serve as an electron donor, with oxygen being an electron
acceptor, and carbon dioxide (CO2) or bicarbonate (HCO3") as the carbon source (Santim, Sly,
Schnagl, and Macy, 2000)
This discovery is important because it can help those interested in the bioremediation of arsenic
understand the factors that affect remediating arsenic. Studies show that microorganisms can
contribute to the toxification of arsenic. A site filled with arsenite-oxidizing bacteria will create
more toxic forms of arsenic and increase the ineffectiveness of bioremediating arsenic (Santini,
Sly, Schnagl, and Macy, 2000). Until the discovery of an organism or procedure that can
counteract arsenic transformation, bioremediation of arsenic will remain ineffective and costly.
Materials and Methods
Samples of arsenopyrite were extracted from a gold mine in the Northern Territory of Australia
m order to isolate arsenite oxidizers. (Santini, Sly, Schnagl, and Macy, 2000) Small pieces of
rock were placed m bottles containing a minimal enrichment medium with arsenite absent The
bottles were sent to a laboratory and incubated for 7 days at 28°C (Santini, Sly, Schnagl, and
Macy, 2000). The enrichments were sub-cultured twice into an enrichment medium containing
10mm of arsenite (Santini, Sly, Schnagl, and Macy, 2000).
Growth of Pseudomonas arsenitoxidans
The Pseudomonas arsenitoxidans (NT-26) was grown in a growth medium consisting of 5mm of
arsenite Cultures were grown overnight under experimental medium (200 mL). Portions of the
samples were centrifuged (15,000 x g, 10 minutes) in a microfuge to remove cells and the
supernatant was frozen until analysis was performed (Santini, Sly, Schnagl, and Macy, 2000)
Analytical Methods of Arsenite
The concentration of arsenite was determined using a Vanan spectra AA20 atomic absorption
spectrophotometer. Arsenate was analyzed using a high-pressure liquid chromatography (HPLC)
(Santini, Sly, Schnagl, and Macy, 2000)
Results and Conclusion
It was discovered that Pseudomonas arsenitoxidans NT-26 grew chemolithoautrophically
(Santini, Sly, Schnagl, and Macy, 2000) Pseudomonas arsenitoxidans NT-26 is a gram-negative
motile rod with two subterminal flagella (Santini, Sly, Schnagl, and Macy, 2000). This organism
17
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
gains energy for growth from arsenite oxidation Also, this organism is able to grow in the
presence of organic matter and oxidized arsenite throughout the growth process (Santini, Sly,
Schnagl, and Macy, 2000) In addition, the growth of this organism continues to thrive in the
presence of different yeast extract It also was observed that the amount of growth was greater in
the presence of arsenite, which suggests that the organism is gaining energy from the oxidation
reaction and from the oxidation of yeast extract (Santini, Sly, Schnagl, and Macy, 2000)
From these results it can be concluded that Pseudomonas arsemtoxidans NT-26 is an arsemte-
oxidizmg bacterium that gains energy from arsenite oxidation Pseudomonas ardenitoxidans NT-
26 is a unique organism because it has the ability to grow rapidly with arsenite chemolitho-
autotrophically. This discovery is significant because many arsenic oxidizing organisms have
been discovered, such as Bacillus arsenoxydans and Alcaligenes Faecahs, but none of these
organisms have the ability to grow chemolithoautotrophically Therefore, the discovery of this
bacteria species is an advancement in the understanding of microbial-arsenic interactions. The
more knowledge acquired about microbial-arsenic interactions, the more researchers will begin
to understand the benefits and limitations of bioremediating arsenic
Summary
Arsenic is found in many parts of the environment and is toxic to the environment It is the
number one contaminant found in Superfund, Department of Defense, and Department of Energy
sites. Exposure to arsenic can inhibit mitochondria functions and decrease the cellular production
of ATP. Fever, anorexia, melanosis, and cardiac arrhythmia are caused by arsenic exposure
Acute exposure results in sensory loss in the peripheral nervous system. Chronic exposure can
produce changes in the skin epithelium. This toxic element is a public health concern and will
remain a health concern if an effective and innovative way to remove or reduce this toxic
compound in soils, sediments, and groundwater sources is not found
Arsenic is located in Group V of the periodic table It is considered a semi-metallic compound. It
exists in four oxidation states -3,0, +3, +5. Arsenic's oxidation and environmental conditions
affects its mobility and immobility conditions. Under aerobic conditions, arsenate (H3As04,
H2As04", HAs042", As043") precipitates when metal cations are present.
Arsenic (As (V) 0) possesses the ability to co-precipitate iron oxyhydroxides under acidic and
moderately reducing conditions. Arsenic mobility is increased when pH increases However,
arsenic compounds absorb in the soil and can travel short distances in ground and surface water
Arsenite co-precipitates with metal sulfides and has a high attraction to other sulfur compounds.
This fact suggests that arsenic is capable of transforming to an immobilized precipitate form As
stated previously, as pH increases, arsenic's mobility is increased This provides a limitation to
the bioremediation of arsenic because once the compound is immobilized; variations in pH can
reverse arsenic's immobility to mobility.
The case study Mobilization of Arsenite by Dissimilatory Reduction of Absorbed Arsenate
discusses microbes that can mobilize arsenite This expenment discovered that Fe (IH)-respiring
bacterium mobilizes arsenic. The bacterial strains Sulfurospinllum arsenophilum and
Sulfurospirillum barnessn both can release arsenic into the environment. This information
provides knowledge about what organisms mobilize arsenic. This suggests that microorganisms
can be responsible for the spreading of arsenic in groundwater and soil sources. This also
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B lore mediation of Arsenic, Chromium, Lead, and Mercury
confirms that microorganisms hold the ability to transform arsenic As (V) to As (III). This
transformation is not an effective approach to environmental detoxification.
The case study, A New Chemolithoautotrophic Arsenite-Oxidizmg Bacterium Isolated from a
Gold Mine. Phylogenetic, Physiological and Preliminary Biochemical Studies, describes the
discovery of arsenite-oxidizing bacteria that can gain energy in the presence of arsenite
Pseudomonas arsentioxidans is the only organism identified to gain energy in the presence of
arsenite. This organism is capable of growing chemolithoautrophically with arsenite, oxygen and
carbon dioxide. This is a unique organism because most arsenite-oxidizing bacterial are
heterotrophic, not Chemolithoautotrophic. This discovery emphasizes the need to understand
arsenic microbe-metal interactions. Microorganisms can contribute to the toxification of arsenic,
thus limiting the effectiveness of bioremediation This case study also discussed the techniques
used to extract arsenic from contaminated soil and sediments. As stated earlier, the reduction of
arsenate As (V) to As (III) is more toxic and soluble than As (V) (Lovely, 2001) Recent studies
suggest that the As (V) reducing microorganism, Sulfurospmllum barnessi, has the capability to
reduce and mobilize arsenate As (V), which was co-precipitated on fernhydrite.
In addition to metal reduction, the production of organic acids by heterotrophic organisms can
aid with the extraction of arsenic. Moreover, research is studying the ability of filamentous fungi
and yeasts to volatilize arsenic Documents show that several fungi species, such as Gliocladium,
Candida humicola, and Pemcillium species can convert monomethylarsomc acid to
trimethylarsine, different forms of arsenic (Lovely, 2001). This process is descnbed as arsenic
methylation, which involves the transfer of methyl by S-adenosylmethiomne (Lovely, 2001)
The significance of this study is the acknowledgement that fungi species can help remove or
leach metals from industrial wastes Overall, the bioremediation of arsenic involves reduction
and oxidation of arsenic with the use of organisms Based on these findings, the reduction and
oxidation of arsenic is not an effective detoxification method because all arsenic forms are toxic.
However, the use of fungi species may offer a way to leach arsenic from industrial waste sites.
The production of organic acids with the use of heterotrophic organisms and the generation of
sulphuric acid with the use of microorganism, such as Thwbacillus, offers some promising
approach to the extraction of arsenic
References
Agency for Toxic Substances and Disease Registry, ATSDR. 1999 httpV/www atsdrc.dc.gQy.
Derek R Lovely. Anaerobes to the Rescue Science 2001 Volume 293
Joanne M Santini, Lindsay I. Sly, Roger D Schnagl, and Joan M Macy. A New
Chemolithoautotrophic Arsenite-Oxidizmg Bacterium Isolated from a Gold Mine Phylogenetic,
Physiological, and Preliminary Biochemical Studies. Applied and Environmental Microbiology.
2000. p. 92-97
Juerg Zobrist Mobilization of Arsenite by Dissimilatory Reduction of Adsorbed Arsenate
Environmental Science Technology 2000 34, 4747-4753.
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
CHROMIUM
Health and Toxicology
Chromium is found in rocks, animals, plants, and soil The most common forms of chromium are
Cr (II), Cr (III), and Cr (VI) Steel is made from chromium (0) Cr (III) appears naturally in the
environment Industrial processes produce chromium (II) and chromium (VI). The hexavalent
chromium compounds (chromium VI) are industrial produced by the oxidation of chromium (II)
compounds (ATSDR, 1999). The toxicokmetics of a given chromium compound depends on the
valence state of the chromium compound (ATSDR, 1999)
Adsorption from Cr (VI) compounds is higher than Cr (III) compounds because chromate anion
(CrO4)2" enters the cells by facilitated diffusion (ATSDR, 1999) By passive diffusion and
phagocytosis, adsorption of chromium III atoms can take place (ATSDR, 1999). Oral exposure
to chromium occurs when it is inhaled; adsorption occurs in the lung and transfers across the cell
membranes and into the gastrointestinal tract (ATSDR, 1999) Adsorption that occurs through
oral exposure in humans varies because chromium particles vary in solubility (ATSDR, 1999).
Dermal adsorption depends on physical and chemical properties of the compound, the fomite,
and the skin type Solutions that are concentrated with chromium (VI) compounds, such as
potassium chromate, cause chemical bums (ATSDR, 1999).
Once chromium enters the blood stream, chromium compounds can be distributed to all organs
of the body. Cr (VI) is unstable in the body and is reduced ultimately to Cr (III) by many
substances like ascorbate and glutathione (ATSDR, 1999) Once this reduction occurs, excretion
can occur through urine, hair, and nails. However, hair and nails provide minor pathways of
excretion (ATSDR, 1999). Studies suggest that toxicity effects of Cr (VI) compounds result from
the destruction of cellular components Destruction of cells is caused by generation of free
radicals Chromium is a compound that should be dealt with caution because of its toxic effects.
Chemical and Physical Characteristics
Chromium only exists naturally on earth in its compound form, not in its elementary form
(Evanko and Dzombak, 1997) Cr (VI) is the form of chromium that is mostly found at
contaminated sites. This compound is the dominant form of chromium and is found in shallow
aquifers, an aerobic environment Cr (VI) can be reduced to Cr (HI) by organic matter and
components like S2- and Fe 2+ under anaerobic conditions Anaerobic conditions often exist in
groundwater (Evanko and Dzombak, 1997) Precipitation in the presence of metal cations, like
lead, usually exists with chromium (VI) species like chromate (Cr042") and dichromate (CrllO?2")
Adsorption on soil surfaces composed of iron and aluminum oxides usually occurs with
chromate and dichromate. At a low pH (<4) Cr (III) is the dominant form of chromium Cr (III)
forms solution complexes with Ntfc, OH", Cl", F", CM", S042" and with soluble organic ligands
(Evanko and Dzombak, 1997).
Chromium mobility depends on the sorption characteristics of the soil and the amount of organic
matter present. Cr (VI) is more toxic and more mobile than all the forms of chromium.
Chromium (III) is also mobile, but it decreases with adsorption of clays and oxide minerals
below a pH of 5 (Evanko and Dzombak, 1997). Chromium can be transported via surface runoffs
to surface waters while it is soluble or when it in its precipitated form The increase in soil pH
increases the teachability of Cr (VI) Usually when chromium is released into natural waters it is
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
deposited into the sediment where bioremediation of this metal can occur (Evanko and
Dzombak, 1997)
Case Study: Reduction and Precipitation of Chromate by Mixed Culture Sulphate-
Reducing Bacterial Biofilms
Introduction
Industries such as leather tanning, petroleum refining, and pulp production generate large
quantities of chromium in the environment The increase in chromium pollution has been
recognized and has caused a demand to produce chemical and biological remediation techniques
(Smith and Gadd, 2000).
Recent studies have shown that microorganisms are capable of altering the redox state of toxic
metals, organometals, and radionuclide contaminants. Chromium is a metal compound that
possesses a high oxidation state and high solubility. Chromium has many oxidation states, from -
2 to +6 These characteristics make the remediation process of chromium difficult (Smith and
Gadd, 2000)
It is now recognized that sulphate-reducing bacteria are obligate anaerobic heterotrophs and can
cause toxic metals to precipitate to insoluble metal sulphides. It is also known that biofilms can
affect the sorption, transportation, and decomposition of metal and radionuclide pollutant species
(Smith and Gadd, 2000). These techniques are used to remediate chromium despite their
challenges
The purpose of this experiment is to determine the ability of sulphate-reducing bacterial biofilms
to reduce hexavalent chromium and to analyze the effects of Cr (VI) on sulphate (Smith and
Gadd, 2000)
Materials and Methods
Sulphate-reducing Bacteria
The mixed culture of sulphate-reducing bacteria was used as the organism for the experiment.
The biofilm cultures were sub-cultured at 14-day intervals (Smith and Gadd, 2000).
Biocell
The biocell consisted of a 13x20 mm Perspex anaerobic system with an inflow and outflow
containing 7x14 cm Plastikard polystyrene sheeting (Smith and Gadd, 2000).
Chromium
The chromium assay was analyzed using a Pye Unicam SP9 atomic absorption
spectrophotometer (Smith and Gadd, 2000)
Sulphate
The sulphate in this experiment was assayed by a Metrohm 690 ion chromatograph with a 697 1C
pump, and a 750 auto sampler with PRP-X100 column (Smith and Gadd, 2000).
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Sulphide
The sulphide was assayed by DC polarography using a Metroham 663 VA polarograph stand,
and Polarecord recorder (Smith and Gadd, 2000).
Protein
The bacterial biofilm was analyzed by measuring the protein with the Bradford method (Smith
and Gadd, 2000).
Results and Conclusion
This experiment demonstrated that Cr (VI) is converted to an insoluble, less toxic trivalent form
with the use of mixed culture of sulphate-reducing bacterial biofilms In basic solution,
chromium forms a yellow chromate ion (CrO42~). Therefore the reduction of chromate causes a
decrease in the yellow chromate ion It was noted only a small percentage of chromium remained
in solution Cr (VI) at 48 hours (Smith and Gadd, 2000) The mass balance studies showed that
the majority of the chromate was transformed to an insoluble form, which accumulated at the
base of the biofilm The bacterial biofilm did not retain a lot of the chromium In this study, the
mixed culture sulphate reducing bacterial biofilms were capable of reducing 88% of the total
hexavalent chromium over a 48-hour time period This experiment suggests that the biological
mechanism of Cr (VI) reduction is similar to that found in other sulphate-reducing bacterial
biofilms (Smith and Gadd, 2000).
Case Study: Bioremediation of chromium with Cheese Whey (Rynk, 2004)
Introduction
The city of Emeryville is concerned about the future use of its groundwater and the health risks
of construction workers who are responsible for removing soil from a local site (Rynk, 2004)
The new owner of the site obtained a loan from Emeryville under the city's Capital Incentives
for Emeryville's Redevelopment and Remediation program to conduct an engineering
evaluation/cost analysis to determine remediation options for groundwater contaminated with
hexavalent chromium Three remediation approaches were evaluated' natural attenuation,
excavation followed by pump-and-treat, and bioremediaton using cheese whey.
Upon evaluation, it was determined that natural attenuation of hexavalent chromium would be
inefficient and timely, and excavation followed by pump-and-treat, which would involve
removing and land filling the hexavalent chromium-contaminated soil and pumping and treating
the contaminated groundwater plume, would eventually result in contamination of the waste
stream (Rynk, 2004) However, results of a pilot test involving the injection of cheese whey into
the soil and groundwater showed that this method would be the most effective and cost efficient
alternative The use of cheese whey is an attempt to use an inexpensive nutrient supply to
nourish microorganisms that possess the ability to convert hexavalent chromium to tnvalent
chromium (Rynk, 2004) The pilot test produced a 99% decrease of hexavalent chromium in
several of the wells over a 2-month penod (Rynk, 2004)
Site Description
The site is in a commercial/industrial land located in Emeryville, California, which is located
approximately ten miles northeast from San Francisco (Rynk, 2004). This site operated as a
chrome-plating facility from 1951 through 1967 (Rynk, 2004)
»
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Bioremediation Process
The full-scale remediation of the site was scheduled to begin in early 2004 and involved monthly
injection of diluted cheese in 22 injection wells Fifteen thousand gallons of cheese whey was
required to conduct the project (Rynk, 2004). Each monthly injection consisted of diluting the
cheese whey with water The dilution and injection was conducted using portable injection
trucks After the dilution process, 300 gallons of diluted whey solution was injected into each of
the 22 injection wells Each monthly injection took eight days to complete (Rynk, 2004).
Monitoring of the Chromium
In order to monitor the effectiveness of the remediation, the groundwater was monitored for total
hexavalent chromium and other chemical indicators, such as pH, oxygen-reduction, dissolved
methane, and oxygen (Rynk, 2004) The monitoring wells were placed around the plumes.
(Rynk, 2004)
Conclusion
Studies show that in situ groundwater remediation can increase growth of microorganisms and
provide effective treatment The organic matter used during the remediation process depleted the
oxygen levels and thus shifted the environment to an anaerobic atmosphere Furthermore, the
organic compounds can serve as an electron donor for a chemical reduction process (Rynk,
2004) This project demonstrated that cheese whey is an important component to the remediation
of hexavalent chromium.
Summary
Chromium is a compound that is found in nature. The common forms of chromium are Cr (II),
Cr (III), Cr (VI) This section of the paper discussed how the chemical and physical properties of
chromium contribute to health effects it causes and the techniques used to bioremediate this
compound
In regards to health effects, Cr (VI) is the main contributor to generating health problems
Inhalation exposure to Cr (VI) begins with the lungs and spreads across cell membranes. Cr (VI)
can also gain entry to the body by dermal exposure For example, solutions concentrated with
potassium chromate can causes chemical bums These bums provide a dermal pathway. Studies
support that toxicity effects of Cr (VI) result in the destruction of cellular components It is
important to study chemical and physical characteristics of chromium to understand its toxicity
effects.
This section also discussed the effects valence has on the mobility and immobility of chromium.
Chromium (VI) is more toxic and more mobile than all other forms of chromium Cr (III) is less
mobile, especially in the presence of clays and oxide minerals below a pH of 5. Studies
consistently show that organic matter, pH of sediments affect the mobility of chromium Organic
matter and pH can convert Cr (VI) to Cr (HI), causing a decrease in mobility Microorganisms
and other environmental factors, such as pH, climate, and presence of minerals, contribute to the
immobility or mobility of chromium
The case study "Reduction and Precipitation of Chromate by Mixed Culture Sulphate-Reducing
Bacterial Biojilms" further discusses the effects microorganisms have on chromate Recent
studies have shown microorganisms are capable of altering the redox state of toxic metals.
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
organometals, and radionuclide contaminants. Chromium is a compound that possesses a high
oxidation state and high solubility. Chromium has many oxidation states from -2 to +6. These
characteristics create a challenge for the bioremediation of chromium.
This experiment discovered that Cr (VI) is converted to an insoluble, less toxic Cr (III) with the
use of sulphate-reducing bacterial biofilms. In this study, the mixed culture, sulphate-reducing
bacterial biofilms, reduced 88% of the total hexavalent chromium over a 48-hour time period
Based on this study, it is rational to conclude the use of microorganisms is a way to immobilize
and detoxify compounds, such as chromium
"Bioremediation of Chromium with Cheese Whey" featured a case study in Emeryville,
California, where cheese whey was used to treat groundwater contaminated with Cr (VI) Each
month, cheese whey was injected into 22 injection wells. These wells were monitored with wells
located around the plumes to determine the effectiveness of the remediation This project
emphasizes the importance of microorganisms to the detoxification of chromium
Overall, experiments and literature suggest that biofilms and strains of Pseudomonas putida
assist with the reduction process of chromium. The application of these organisms is mostly done
in the labs This means that the bioremediation of chromium is still at lab scale because more
research needs to be done on the preventing leaching of this contaminant. The microbial and
chromium interaction is still not completely understood, until it is understood, researchers will
remain skeptical of using bioremediation to remediated chromium.
Further Remediation Techniques for Chromium
Studies show new bacterial strains extracted from contaminated sites in Pakistan ho Id the ability
to reduce toxic Cr (VI) to Cr (III) (Lloyd and Lovely, 2001). Biofilms consisting of sulfate-
reducing bacteria also hold the ability to reduce and precipitate Cr (VI) Research also shows that
microbial consortium is able to couple oxidation of phenol to Cr (VI) (Lloyd and Lovely, 2001)
Purification of chromate reductase can occur with the use of bactena strain Pseudomonas putida
(Lloyd and Lovely, 2001). Remediation of chromium is moving toward discovering more
bacteria strains that can reduce Cr (VI)
References
Agency for Toxic Substances and Disease Registry, ATSDR 1999 hitp //www alsdr cJc gov
Cynthia R Evanko, Ph.D., and David A. Dzombak, Ph.D. Remediation of Metals-Contaminated
Soil and Groundwater. GWRTAC. October 1997. www.gwrtac ore
Curtis D Klaassen and John B. Watkms III Absorption, Distribution, and Excretion of
Toxicants Karl K Rozman Essentials of Toxicology. 2003
Jonathan R. Lloyd and Derek R. Lovely. Microbial Detoxification of Metals and Radionuchdes
Current Opinion in Biotechnology. 2001 12-248-253
Robert Rynk. Bioremediation with Cheese Whey. Journal of Composting and Organics
Recycling. 2000 45, 26.
24
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
W L. Smith and G.M Gadd. Reduction and Precipitation ofChromate by Mixed Culture
Sulphate-reducing Bacterial Biofilms. Journal of Applied Microbiology. 2000.
25
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
LEAD
Health and Toxicology
Lead is commonly found in industrial settings and lead exposure has the tendency to cause
adverse health effects. The adverse health effects induced by lead exposure are dependent on two
important components* dose (how much of a contaminant) and duration (how long there has been
contact with the contaminant) (ATSDR, 1999). Exposure to lead can occur from eating and
drinking contaminated water or breathing in high levels of lead (ATSDR, 1999). Children are
exposed to lead through eating lead based paint chips or through skin absorption while playing in
lead contaminated soil This hazardous material enters the air we breathe, the water we dnnk,
and the plants and animals we consume.
Lead is a health problem for communities that reside near acidic water supplies Acidic water can
cause lead to leach into our drinking water supply from lead pipes, leaded solder, and brass
faucets. The EPA reports that more than 99% of the public's drinking water contains less than
0 05 parts per million of lead, which is the maximum contaminant level for lead (ATSDR, 1999)
Refining facilities, brass foundries, rubber refineries, and steel welding operations are settings in
which exposure to lead to optimal The Agency for Toxic Substances and Disease Registry
(ATSDR) states between 0 5 and 1 5 million workers are exposed to lead at the work place.
More than 200,000 workers in California alone are exposed to lead (ATSDR, 1999) This
suggests that many people, especially blue-collar workers, are disproportionately exposed to lead
and suffer adversely from health problems because of it.
Once lead has entered the blood stream, it flows to various parts of the body. Lead most
commonly enters the body through ingestion, and then travels to the lungs then swiftly through
the blood stream to other parts of the body Lead does not change form once it enters the body
Lead in the blood stream travels to the soft tissues of the body, such as the liver, kidneys, lungs,
brain, spleen, muscles, and heart Over time, lead particles have the ability to move into the
bones and teeth Lead impairs communication between cells and modification of neuronal
circuitry (Curtis D. Klassmen and John B Watkms 111, 2003) ATSDR reports that 94% of the
total amount of lead in the body is contained in bones and teeth For children, 73% of lead in
their body is stored in their bones (ATSDR, 1999). Lead that enters the bones can remain there
for decades. However, over time, lead that is stored in the bones can reenter the blood stream and
organs.
Excretion of lead occurs through urine and feces when it is not stored in the bones. ATSDR
reports that 99% of the amount of lead absorbed in an adult's body will leave the body through
waste within a couple of weeks In contrast, only 32% of the lead absorbed by children's bodies
leaves as waste Means of exposure determine if the lead entering the body will be removed or
accumulate in the body tissues, especially within the bone (ATSDR, 1999)
Lead toxicity usually begins with the nervous system in both adults and children Exposure to
lead can cause weakness in fingers, wrists, or ankles Studies show long-term exposure to lead at
work can lead to decreased performance. High levels of lead can cause damage to the brain,
kidney, and can cause anemia in both adults and children (ATSDR, 1999)
26
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Lead produces a range of different effects that vary in adults and children A child that ingests
large amounts of lead may develop blood anemia, kidney damage, colic, muscle weakness, and
brain damage. These combined health effects can cause death in children However, low levels
of lead contamination can also cause detrimental effects to the blood and brain of a child.
Pregnant women can experience a miscarriage if exposed to high levels of lead during there
pregnancy (ATSDR, 1999) High levels of lead can also damage organs that are responsible for
sperm production Consistent and long exposures to lead can potentially produce noxious health
effects.
Chemical and Physical Characteristics
It is essential to understand the chemical and physical characteristics and processes of a metal to
understand its full bioremediation potential. The mobility and immobility of compounds is
dependent on their physical and chemical characteristics. These two characteristics dictate a
metal's particular mechanism
Different conditions allows for lead to reside in different forms. In nature, lead exists with an
oxidation state of 0 or +11. Lead possesses a molecular weight of 207.20 and is a bluish-gray
colored solid It has a melting point of 327.4 and a boiling point of 1,740 °C. Lead (Pb2+) and
lead hydroxy compounds are the most stable form of the lead However, Pb2+ is the most reactive
and common form of lead. This form produces mononuclear and polynuclear oxides and
hydroxides (Evanko and Dzombak, 1997)
In the presence of inorganic compounds (such as Cl, COs2", SCV, PC>43~) and organic ligands
(such as hurmc, folic acids, EDTA) low soluble lead compounds form The concentration of
dissolved salts, pH, and minerals affect the amounts of lead found in surface water and
groundwater In surface water and groundwater systems, lead can appear in its precipitate form
(PbCOa, PbzO, Pb(OH)2, PbSO4) and is capable of absorbing into the surface of minerals
(Evanko and Dzombak, 1997) Adsorption, ion exchange, precipitation, and complexanon with
organic matter are mechanisms that limit the amount of lead leaching through surface water or
groundwater Tetramethyl lead, the volatile organolead compound, forms in anaerobic sediments
by an alkyllation mechanism (Evanko and Dzombak, 1997). Volatile lead creates unsafe
conditions for water supplies because leaching is prominent in this form In most cases, a
precipitated form of lead is the more desirable form because the lead is immobilized
Subsurface Mobility and Immobility
In situ bioremediation is the application of remediation, which occurs below the ground or at the
site Subsurface bioremediation refers to remediation that occurs above the groundwater source.
The subsurface of the earth is composed of a vapor phase and a capillary fringe above the ground
surface. Subsurface bioremediation usually involves the remediation of unsaturated soil or soil.
Bioventmg, a process that requires a supply of air or oxygen to soil to stimulate aerobic
biodegradation of contaminants, is an example of subsurface bioremediation Components like
adsorption, ion exchange, precipitation, and complexation with organic matter are factors that
contnbute to the high adsorption rate of lead in soil (Evanko and Dzombak, 1997). These
components also contnbute to the immobilization of lead on surface water or groundwater.
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Groundwater Bioremediation
Groundwater is water that is located below the earth's subsurface. Groundwater is usually
located near the residual saturation point. Groundwater bioremediation can either occur in situ or
ex situ
Aerobic and anaerobic reactions are also examples of in situ groundwater processes. An aerobic
process involves air or oxygen placed into the groundwater in order to promote biodegradation of
contaminants (USEPA Office of Solid Waste and Emergency Response, 2001). Anaerobic
process consists of a carbon source such as molasses, lactic acid, or hydrogen release compounds
are injected into groundwater to enhance biodegradation of contaminants (USEPA Office of
Solid Waste and Emergency Response, 2000)
Case Study "Dynamics of Lead Immobilization in Sulfate Reducing Biofilms"
(Beyenal and Lewandowski, 2004)
Introduction
The purpose of this study is to examine the effects that minerals (specifically quartz and
hematite) have on a biofilm consisting of Desulfovibro desulfuncans (Beyenal and
Lewandowski, 2004). Quartz and hematite were selected to produce mineral-metal interactions.
Studies support that under anaerobic conditions the bacterium Desulfovibrio desulfuncans is
capable of immobilizing lead In this experiment, the minerals released iron ions Iron (Fe HI)
can be reduced by sulfate-reducing bactena (Beyenal and Lewandowski, 2004)
Although the transformation of Fe (III) to Fe (II) was not quantified in this article, the chemical
process where sulfate-reducing bacterial biofilm removed lead was observed Sulfate-reducing
bactena generated energy by the oxidation of organic compounds and the reduction of sulfate
ions (Beyenal and Lewandowski, 2004). This chemical process produces H2S and then
dissociates into bisulfide Since lead is a component of this expenment, lead ions were present
during the chemical reaction. The lead ions m the presence of bisulfide caused the bisulfide to
precipitate lead ions as lead sulfide (PbS). Minerals were also in the presence in the process
described above. The minerals found near the metal ions served as nourishment for the biofilm
(Beyenal and Lewandowski, 2004). Another component of this reaction is oxygen It was
realized by the researchers that once metal sulfide is immobilized, the introduction of oxygen
would potentially cause remobilization of the metal hydroxides, oxides, and sulfates These
factors demonstrated that the stability of lead is affected by the composition of the biofilm, the
minerals present, and the presence of oxidants (Beyenal and Lewandowski, 2004). In summary,
this expenment attempted to bnng understanding to the dynamics of biosorption of lead, which
involves the passive accumulation of metals or radioactive material by biological organisms
(Lovely, 2001).
Materials and Methods
Organism
Desulfovibrio desulfuncans was used to grow the sulfate-reducing bacterial biofilm. The
experiment consisted of D. desulfuncans batch cultures placed in 100 mL serum bottles for three
days. The reactors were inoculated with 20 mL of D. desulfuncans (Beyenal and Lewandowski,
2004).
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Sulfate-Reducing Biofilms
The sul fate-reducing biofilms came from a pure culture of D. desulfiiricons (Beyenal and
Lewandowski, 2004) Batch cultures were created by growing D desulfuncans in 100 mL serum
bottles for 3 days (Beyenal and Lewandowski, 2004)
Growth of Biofilm
The biofilm was grown using a metal-toxicity medium. This metal-toxicity medium reduced the
lead complexation and precipitation Once the metal-toxicity medium was prepared, the solution
was adjusted to 7 2 using IN HCL 0 1 g ascorbic acid, and 0.1 g sodium thioglycollate was
added to a liter of growth medium (Beyenal and Lewandowski, 2004) This mixture was
designed to stimulate the growth of the sulfates-reducing bactena.
Reactors
The reactors consisted of two flat plates; one filled with hematite and the other with quartz The
reactors had a dimension of 3 5 cm deep, 2 5 cm wide, and 34 cm long with a total working
volume of 120 mL The quartz chips were 2.5x7 5x 0.1 cm3 and the hematite chips were 0 5 xO.5
cm3 (Beyenal and Lewandowski, 2004). The hematite chips were cleaned with acetone and
rinsed twice with distilled water and polished with sandpaper After the minerals were cleaned,
they were placed at the bottom of the reactors This concluded the preparation of the reactor,
analysis of the minerals proceeded after the preparation
The minerals were analyzed with the use of an X-ray diffraction tool. This x-ray diffraction tool
determined that the hematite chips were 90% hematite and 10% magnetite and contained traces
of cristobalite minerals (Beta-SiO2) (Beyenal and Lewandowski, 2004)
Quantifying Lead and Iron Ions
In order to determine the total lead and iron concentrations in the biofilms, the biofilm was
removed from the chip and placed in distilled water for two minutes. It was then treated with
three-N nitric acid Then nitric acid (HNOa) was used to dissolve the precipitated metals
(Beyenal and Lewandowski, 2004) Lastly, the samples were centnfuged for 20 minutes at 6000
rpm After centrifugation, the pellet was discarded and the supernatant was used for iron and lead
analysis
Results and Discussion
The data from this experiment demonstrate that lead was completely immobilized with the
biofilm grown on the quartz. However, the biofilm grown on hematite did not immobilize all of
the lead (Beyenal and Lewandowski, 2004). In addition, when the biofilms were exposed to
atmospheric oxygen, the rate of lead immobilization decreased.
Biofilms grown on the hematite and on the quartz were heterogeneous because they were
composed of cell clusters (Beyenal and Lewandowski, 2004) The average hydrogen sulfide
concentration was higher m the biofilms grown on quartz than in the biofilms grown on hematite.
Furthermore, during the 18 weeks of operation, iron was continuously being released from the
hematite Lead precipitated more from the biofilms grown on quartz, which may suggest that
microorganisms present on some minerals, like quartz, allow for efficient precipitation of lead.
The results also suggest that metals released from redox-sensitive minerals can precipitate in the
presence of microbial- generated hydrogen sulfide and decrease the metal-binding capacity of the
contaminant metal (Beyenal and Lewandowski, 2004)
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Conclusion
The results show that the maximum efficiency of metal immobilization in sulfate-reducing
biofilms can be accomplished when minerals are redox-insensitive and located in the earth's
subsurface (Beyenal and Lewandowski, 2004) Final results suggest that metals released from
redox-sensitive minerals can precipitate in the presence of hydrogen sulfide and then decrease
the overall metal-binding capacity of the system This process, which involves hydrogen sulfide
binding to metals released from minerals, occurs because of the biofilm's heterogeneity and
kinetic limitations
This research demonstrates that lead precipitates more in the presence of biofilm that is located
near redox-insensitive compounds, such as quartz Quartz does not release many metal ions As
previously mentioned, metal ions released from a mineral can decrease the metal-binding
capacity of the compound
Biofilm grown on hematite can cause a decrease in lead precipitation The minerals also
contnbute to the amount of hydrogen sulfide released According to the microelectrodes, the
biofilm located on quartz produced higher concentrations of hydrogen sulfide (H2S), unlike the
hematite that generated smaller concentrations of hydrogen sulfide. The concentration of
hydrogen sulfide affected the growth level of the biofilm, which affected the lead's binding
capacity. The results obtained from this experiment demonstrate the effects of sulfate-reducing
biofilms in the presence of minerals on lead immobilization
Case Study: Metal Bioremediation Through Growing Cells (Malik, 2003)
Introduction
This journal article, written by Anushree Malik, describes the beneficial use of bacterial and
fungal strains in removing metal pollution Studies show that bacteria, yeast, and fungi
specimens have the capability of extracting metal contaminants from sites that are saturated with
metals like cadmium, copper, nickel, cobalt, zinc, and lead Industries are seeking low-cost and
efficient methods of removing metals from contaminated sites
Industries use physico-chemical processes, such as oxidation and reduction, chemical
precipitation, filtration, electro-chemical treatment, evaporation, ion exchange, and reverse
osmosis, to remove lead However, these processes are limited because some sites contain
contaminants that are not affected by these processes Studies show that contaminating reagents
cause desorption, which in turn causes toxic sludge and secondary environmental pollution
(Malik, 2003) These limitations further explain the extensive search to find more cost-effective
processes to remove metal pollution
Biotechnology provides a cost-efficient way to remove hazardous material, and can be effective
at selectively removing metals. Biotechnology involves the use of organisms to respond to
pollution through techniques like biosorption, precipitation, complexation, and oxidation-
reduction reactions
Biosorption, the passive uptake of heavy metal ions, involves the use of bacterial compounds to
remove abstract metals or radioactive elements from contaminated sources Studies show that
heavy metals can be biosorbed into binding cites present in cellular structures Biosorption alone
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
is not sufficient for effective metal remediation. Growing cells have unlimited capacity to bind to
organo-metallic complexes, degrade organic compounds, and take up other inorganic ions such
as ammonium (Malik, 2003) This study shows that biosorption accompanied with active and
growing cells may provide a better way of removing metals.
Precipitation involves metal cations and anions (e.g., S042", S2", oxalate, HPO42") forming
insoluble aggregates (salts or complexes), such as sulfides, carbonates, oxides, and oxalates.
Complexation involves metabolites and siderophores that cause metal mobilization (Malik,
2003) Oxidation and reduction are chemical reactions that involve an organic contaminant that
is oxidized (loses electrons), and a chemical or an organism that is reduced (gains an electron).
The organic contaminant that is oxidized is called the electron donor and the chemical or
organism that is reduced is called the electron acceptor. The energy gained from the electron
donor is stored and used to produce more cells for the electron acceptor
Results and Conclusion
The experimenter focused on where the bioaccumulation of metals occurs in living cells. The
location and resistance mechanism is dependent on the straia This experiment discovered that
Pseudomonos marginalis, a bacterial strain, isolated from lead-contaminated soil produced a
higher resistance and extracellular lead with high extracellular polysacchande production
Bacillus megaterium, another bacteria strain, isolated from soil containing high soluble lead
demonstrated lower resistance and intracellular accumulation of lead This result confirmed that
bacteria, fungi, and any active cell can react differently to hazardous materials It also confirmed
that the bioaccumulation of lead occurs in different portions of a cell.
Plectonema boryanum, an organism, confirmed that poly phosphate bodies absorb zinc, lead,
manganese, and aluminum Polyphosphate bodies are components of living cells that participate
in the active uptake of metals The experiment on this strain suggested that lead could be found
in the cytoplasm of cells When lead entered Saccharomyces cerevisiae cells, most of the lead
was found in the cytoplasm in two hours. Observing the interaction of the lead and
Saccharomyces cerevisiae and Plectonema boryanum explained the route of lead absorption
First, lead enters the cell and binds to the cell wall Then, lead accumulates in the cell wall
Finally, it travels to the cytoplasm (Malik, 2003)
This experiment demonstrated that lipolysaccharides absorb metals like cadmium, copper, lead,
and zinc. The organism Bradyrhizobium jopomcum confirmed that a lipololysacchande mutant,
lacking o-polysacchande, binds 50%-70% less than a non-mutant lipololysacchande. This
suggested that lipololysaccharide molecules ofB. japomcum affect the precipitation of metals
(Malik, 2003) Components of living cells affect the biosorption of metals
This study also examined the bacterial stain Desulfosporosmus orientis, to identify its ability to
biosorb metals. Contaminants, such as lead, cadmium, zinc, and copper, were found in
groundwater located at a car battery recycling plant. Groundwater contamination often requires a
different remediation approach than surface water. The experimenters discovered that
Desulfosporosmus orientis assists with sulfate reduction and causes complete precipitation of
metal sulfides. This discovery helps with the advancement of bioremediation of metals, such as
lead, cadmium, zinc, and copper, located in groundwater sources
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Metal pollution endangers the vitality of the world's ecosystem These organisms used in the
experiment offer a promising resolution to preserving the ecosystem Studies show that
biosorption of metals with the use of dead biomass are not effective removal techniques for
recalcitrant metals, such as nickel (Malik, 2003). Therefore, the use of lively metal-resistant
bacterial/fungal strains may be a more effective way to remove metal contaminants. Active
bacterial/fungal strains are able to function in extreme pH conditions and high metal
concentrations, thus providing a practical and economical solution for the removal of metals
(Malik, 2003).
Summary
Lead is a metal compound that can be found in all parts of the environment Lead is a product of
industrial activities like mining and manufacturing Lead is a metal contaminant prevalent in
Superfund, Department of Energy, and Department of Defense sites (Malik, 2003) It is a metal
compound that needs to be removed as soon as possible from water and land sources around the
United States
Concern over lead's detrimental health effects contributes to research that concentrates on the
bioremediation of lead. High levels of lead can cause damage to the brain, kidney, and anemia in
both adults and children (Malik, 2003). Exposure to lead can cause premature births in pregnant
women Thus, the data show that lead is a contributor to health problems in the United States
Studies like the Dynamics of lead immobilization in sulfate reducing biofilms help researchers
understand how lead can be immobilized in groundwater This journal article showed how
minerals affect the precipitation of lead. Minerals like quartz and hematite affect the precipitation
level of lead. Sulfate-reducing biofilms are also affected by minerals (Malik, 2003)
Understanding the effects of minerals and biofilms can help others find effective ways of
removing lead from contaminated sites
The article Metal bioremediation through growing cells demonstrated that many bacterial and
fungal strains can assist with lead removal Organisms like Pseudomonas marginahs,
Plectonema boryanum, and Desulfosporiosmus orients can all assist with lead uptake (Malik,
2003). This suggests that bioremediation using growing microbes is a practical alternative to
biosorption removal of metal contaminants from industrial sites
Research today is focusing on the precipitation of metal sulfides utilizing sulfate-reducing
bacteria. The Mine Waste Technology Program of the United States Environmental Protection
Agency and Department of Energy uses sulfate-reducing bacteria for bioremediation purposes
They also use passive treatment systems like compost for remediation This is currently found
not to be an effective treatment because the precipitates are not guaranteed to stay in their
precipitated forms (Malik, 2003). Active treatment methods often use sulfate-reducing bacteria
for the production of hydrogen sulfide, which causes rapid precipitation and recovery of metal
sulfides (Beyenal and Lewandowski, 2004).
However, the challenge still exists to find fungal or bacteria biofilms that can work in many
different conditions and keep the metals from leaching into the environment. It is important for
researchers to discover ways to tackle this challenge; otherwise industries will continue to be
skeptical about using bioremediation at metal-contaminated sites. (Beyenal and Lewandowski,
2004)
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
References
Agency for Toxic Substances and Disease Registry, ATSDR. 1999 http //www atsdr cdc gov
Anushree Malik. Metal Bioremediation Through Growing Cells Environmental International
August 2003. Volume 30.
Curtis D. Klaassen and John B. Watkins III. Absorption, Distribution, and Excretion of
Toxicants. KarlK Rozman Essentials of Toxicology. 2003.
Cynthia R Evanko, Ph D , and David A Dzombak, Ph D. Remediation of Metals-Contaminated
Soil and Groundwater. GWRTAC October 1997 www gvvrtac org
Derek R. Lovely Anaerobes to the Rescue Science 2001. Volume 293
Haluk Beyenal and Zbigniew Lewandowski. Dynamics of Lead Immobilization in Sulfate
Reducing Biqfilms. Science Direct June 2004. Volume 38, Issue 11.
Liesbet van Cauwenberghe and Dianes Roote, P.O. In situ Bioremediation GWRTAC. 1998
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
MERCURY
Health and Toxicology
Metallic mercuiy has been found at 714 hazardous waste sites in the United States (ATSDR,
1999). The abundance of mercuiy present within these contaminated sites causes health and
environmental problems to surrounding ecosystems. Chronic exposure to mercury can cause
detrimental effects to human health and the environment Exposure to mercury can come from a
number of different routes, such as through dental amalgam fillings, household products,
fluorescent light bulbs, broken thermometers, and industrial settings Exposure to methyl
mercury by these routes can cause health problems. A major source of exposure to methyl
mercury comes from the consumption of fish Methyl mercury is the most toxic species of
mercury because it possesses a high adsorption rate to membranes. Neurotoxic health effects are
common after exposure to methyl mercury Pregnant women who consume large amounts offish
or who are exposed to high levels of mercury are at an increased risk of exposing their fetus to
methyl mercury. Therefore hospitals and clinics advise pregnant women to limit the amount of
fish they consume Physical symptoms of methyl mercury exposure can be identified by
paresthensia and numbness in the fingers and toes Difficulty swallowing and clumsiness—called
ataxia—are also symptoms of methyl mercury exposure (ATSDR, 1999) After ataxia sets in,
neurasthenia can occur, followed by fatigue and an inability to concentrate As methyl mercuiy
continues to poison the body, tremors and vision and hearing loss develop The final stage of
methyl mercury poisoning is coma followed by death (ATSDR, 1999). Methyl mercury is a
substance that should be addressed with caution and advisory because of its harmful health
effects.
Mercury in the form of mercuric chloride is an example of a mercuric salt Oral ingestion of
mercuric salts is said to cause severe abdominal cramps and bloody diarrhea (Klaasen and
Watkins HI, 2003). These symptoms are usually accompanied by corrosive ulceration and
necrosis of the gastrointestinal tract and renal failure within 24 hours of proximal tubular
epithelium necrosis. Any great exposure to mercury leads to the intake of mercuiy by various
organs of the body. Once mercury is absorbed into the body, its elemental or metallic form is
oxidized forming divalent mercury. Mercury vapor absorbed through inhalation penetrates the
body's red blood cells and is transformed to divalent mercury (Klaassen and Watkins III, 2003).
A portion of inhaled mercury vapor in the form of metallic mercury is transported to more distal
tissues where transformation may occur. Inhalation of mercury vapor at high levels may produce
an acute, corrosive bronchitis and interstitial pneumonitis With increased exposure to mercury,
the body's system begins to show physical signs of effects For example, the fingers begin to
tremor followed by memory loss; severe depression becomes apparent after long exposure to
mercury Mercury toxicity can also cause severe salivation and gingivitis (Klaasen and Watkins
IE, 2003)
Chemical and Physical Characteristics
Mercuiy exists in several forms in the environment. These forms include metallic mercury, also
known as elemental mercury, inorganic mercury, and organic mercury. Mercury is a unique and
rare metal found in nature. It is found in a sulfide ore cinnabar in Spain, Russia, Mexico, Canada,
and Algeria (Evanko and Dzombak, 1997) It is known for its high surface tension, flow
behavior, and its electrical conductivity (NABIR, 2003). It is these characteristics that make
mercury a desirable for use by industries Mercury is used in dental products, thermometers, and
neutron absorbers used by nuclear power plants (NABIR, 2003)
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Department of Energy facilities serve as a source of mercury (NABIR, 2003). Mercury as an
inorganic compound that can exist in three oxidation states (0 in its elemental state, +1 in its
mercurial state, and +2 in its mercuric state) All three of these oxidation states of the inorganic
mercury are hazardous Inorganic mercury can be combined with other compounds, such as
chlorine, sulfur, or oxygen forming white powders or crystals These compounds can be used as
fungicides or as elemental components of skin-lightening creams Most inorganic mercury comes
in the form of mercuric sulfide, which along with mercuric oxide is used as a coloring agent in
tattoos and paints. Methyl mercury is the most common form of organic mercury. This form is
most prevalent in freshwater and salt water Another form of mercury is metallic mercury, which
is used to produce chlorine gas, extract gold from ore, and make dental fillings Metallic mercury
is also used in religious practices in Latin America and Caribbean countries (ATSDR, 1999).
Mercury can possess many forms and hold many industrial uses.
Any mercury transformation or immobilization process is dependent on pH Organic or inorganic
mercury can be reduced to elemental mercury under chemical reducing conditions. At a high pH,
the absorption of mercury increases. Mercury can also participate in co-precipitation with
sulfides, which aids in the removal of mercury from a solution Mercury's (Hg2*) ability to form
strong bonds with many different inorganic and organic ligands suggests that it is very soluble in
its oxidized form
Demethylation of methyl mercury can further assist with the transformation of mercury This
process allows elemental mercury to be transformed under anaerobic conditions. Acidic
conditions (pH< 4) favor the production of methyl mercury, where as higher pH values initiate
precipitation of mercury sulfide HgS (s) (Evanko and Dzombak, 1997). Mercury transformation
processes are important to the advancement and development of bioremediation of mercury
located in groundwater and the subsurface of the earth.
Groundwater Treatment
Surface water treatment methods do not provide adequate treatment for the removal of heavy
metal ions, especially for mercury that is found in groundwater (Vilensky, Berkowitz, and
Warshawsky, 2002) Therefore the remediation of mercury contaminated groundwater requires a
different approach than subsurface remediation
Field Scale: Removal of Mercury from Chemical Wastewater by Microorganism in
Technical Scale (Wagner-Dobler, Von Canstein, Li, Timmis, and Deckwer, 2000).
Introduction
Mercury is a toxic element once it binds to sulfydryl groups of enzymes and proteins. The
combination of mercury and sulfydryl groups can halt cell functions within an organism. This
toxic combination is found in sediments. Aquatic organisms and other organisms are exposed to
mercury when methyl mercury is present in the ground. Organisms that are exposed to mercury
suffer from chronic diseases or death Mercury is harmful to many living species; therefore the
discharge of mercury needs to be prevented Mercury-resistant bacteria offer a possible cost-
effective method for removing mercury This type of bacteria reduces soluble Hg (II) to
insoluble metallic Hg (0) by means of cytoplasrmc enzyme mercuric reductase, which is encoded
by the gene, mer A Reduced mercury changes to small droplets of metallic mercury. This is
promising information for removing mercury from sites (Wagner-Dobler, Von Canstein, Li,
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Timmis, and Deckwer, 2000) This field study attempts to remove mercury from chloralkli
electrolysis wastewater and retain it within a bioreactor
Material and Methods
Bacteria
Four subspecies of Pseudomonas puttda (Spi3, Spi4, Konl2, Elb2), two subspecies of
Pseudomonas stutzeri (Ibu3, Ibu 8), and one subspecies of Pseudomonas fulva ( Spill) were
used as inoculant strains (Wagner-Dobler, Von Canstein, Li, Timmis, and Deckwer, 2000)
Single colonies were picked from plated precultures (500 mL) that were inoculated and grown
for 48 hours at 30 °C in rich liquid medium consisting of sucrose lOg/L, yeast extract 10 g/L,
NaCl 30g/L, Hg (II) 5.0mg/L, sodium phosphate buffer 0.25 M, ph 7.0 (Wagner-Dobler, Von
Canstein, Li, Timmis, and Deckwer, 2000) Pre-cultures were transferred to seven, 15-liter
fermentation vessels containing 3 L of rich liquid medium and antifoammg agent. A fed-batch
fermentation was done for 4 days at 35 °C Cultivation of the inoculant strains stopped after four
days (Wagner-Dobler, Von Canstein, Li, Timmis, and Deckwer, 2000).
Mercury Concentrations
Mercury concentrations were analyzed using two automated instruments from Mercury
Instruments, a company located in Karlsfeld, Germany. The reduction of Hg (II) to Hg (0) was
done by the chemical compound SnCfe (20 g/L) (Wagner-Dobler, Von Canstein, Li, Timmis, and
Deckwer, 2000). Metallic mercury was volatilized into the air, this volatilization was determined
by spectroscopy at 253.7 nm
Bioreactor
Mercury-resistant bacteria and wastewater was added to the bioreactor The initial mercury
outflow concentration was 900 ug/L, representing a retention efficiency of 82% at a mercury
inflow concentration of 5 mg/L This resulted in an average mercury outflow concentration of
306 ug/L for three hours, which represented a retention efficiency of 97% After inoculation, full
mercury removal was achieved without an adaptation period of the bioreactor to the on-site
wastewater (Wagner-Dobler, Von Canstein, Li, Timmis, and Deckwer, 2000)
Conclusion
Mercury-resistant bacteria were used to enzymatically reduce Hg (II) to water insoluble Hg (0)
Seven mercury resistant strains of Pseudomonas were immobilized inside a 700 L packed bed
bioreactor. A neutralized chloralkali electrolysis wastewater consisting of mercury
concentrations of 3-10 mg/L was fed into the bioreactor (0.7 nrVh up to 1.2 ir?/h) Within 10
hours of inoculation of the bioreactor, a mercury retention efficiency of 97% was obtained
Furthermore, Ihe bioreactor outflow concentrations were below 50 ug Hg/L that fulfilled the
discharge limit for industrial wastewater The retention efficiency of the bioreactor was not
affected by the inflow fluctuations, pH, or mercury concentrations (Wagner-Dobler, Von
Canstein, Li, Timmis, and Deckwer, 2000)
The mercury remediation process offers a promising way to extract mercury from polluted
wastewater (Wagner-Dobler, Von Canstein, Li, Timmis, and Deckwer, 2000). This remediation
method is applicable in ambient temperature and requires little electricity, which results in low
operating costs Moreover, this process, entitled "end-of-pipe technology," is effective on a
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
technical scale and applicable for mercury wastewater coming from chloralkali electrolysis
factories, groundwater, soil wash water, and mining waters.
Case Study: Microbial Reduction and Oxidation of Mercury in Freshwater
(Siciliano, O'Driscoll, and Lean, 2002).
Introduction
This experiment investigates the role of microbial mercury oxidation and reduction reactions in
regulating dissolved gaseous mercury in Jacks Lake and Lake Ontario, two freshwater lakes
(Siciliano, O'Dnscoll, and Lean, 2002) No other researchers have examined the role of
microbial activity in regulating freshwater patterns of dissolved gaseous mercury. This
experiment studies the role of microbial activity on dissolved gaseous mercury. The
experimenters hypothesized that microbial activity is the source of dissolved gaseous mercury
and reduces levels of dissolved gaseous mercury (Siciliano, O'Dnscoll, and Lean, 2002).
Materials and Methods
The dissolved gaseous mercury concentrations, microbial mercury reductase, and oxidase
activity was measured between 45 and 90 minutes at two lakes' Jack's Lake, located
approximately 200 km northeast of Toronto, Canada, and Lake Ontario (Siciliano, O'Dnscoll,
and Lean, 2002) Samples of water from Jack's Lake were collected from a fiber glass boat 15
cm below the surface by placing a narrow mouth Teflon bottle directly into the water. A Go-Flo
sampler was used to measure Lake Ontario water (Siciliano, O'Dnscoll, and Lean, 2002) The
dissolved gaseous mercury was analyzed by a Tekran Zero Air Generator through a 1L water
sample contained in a glass graduated cylinder (Siciliano, O'Driscoll, and Lean, 2002). The gas
was analyzed for elemental mercury using a Tekran 2537A instrument The Tekran 2537A is an
instrument that first amalgamates mercury into a pure gold cartridge and then thermo desorbs the
mercury so that a cold vapor atomic fluorescence spectrophotometry can analyze the gas every 5
minutes (Siciliano, O'Dnscoll, and Lean, 2002).
Results and Conclusion
The microbial reduction and oxidation of elemental mercury presents an opportunity to reduce
toxic levels of mercury in aquatic ecosystems. A microbial mercury reduction reaction involving
the conversion of mercury (Hg2*) to Hg (0) was observed. It was observed that the microbial
mercury reductase activity increased when the dissolved gaseous mercury increased The
mercury oxidase activity that involves the conversion of Hg (0) to Hg (2+) increased as dissolved
gaseous mercury concentrations decreased in the mid-afternoon These results suggest that
mercury oxidase activity was linked to hydrogen peroxide Qtkfti) diurnal patterns (Siciliano,
O'Dnscoll, and Lean, 2002)
After hydrogen peroxide was placed in Lake Ontano water, the mercury oxidase activity
increased by 250% By 60 minutes, dissolved gaseous mercury decreased to 28% of its initial
concentration (Siciliano, O'Dnscoll, and Lean, 2002). However, two hours after the addition of
the hydrogen peroxide in the lake, the mercury oxidase activity declined, but the mercury
reductase and dissolved gaseous mercury increased. Four hours after the addition of hydrogen
peroxide, mercury reductase and dissolved gaseous mercury levels returned to their original
levels (Siciliano, O'Driscoll, and Lean, 2002).
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
These results suggest that in the morning, microbial activity produces dissolved gaseous mercury
and the photoreduction of Hg+2. As hydrogen peroxide increases in concentration, a biological
decrease in dissolved gaseous mercury occurred throughout the afternoon (Siciliano, O'Driscoll,
and Lean, 2002) Results of this study helps in the understanding of the effects of microbial
reduction and oxidation of mercury to dissolved gaseous mercury in lakes, which is beneficial to
the study of mercury remediation
Summary
Mercury is a metal that can exist in three forms, metallic (Hg)(0), mercurous (Hg2*), and
mercuric (Hg2+). Toxicity of mercury is due to the ability of both organomercunal compounds
and inorganic forms of mercury and their high affinity to bind to membranes (Lovely, 2001).
Organomercury compounds possess the ability to bind to membranes because they are lipid
soluble These characteristics allow mercury compounds to induce damage to membranes and
inactivate periplasmic and cytoplasmic enzymes within a celL Moreover, breathing mercury
vapor has been documented to cause respiratory and acute renal failure Organomercunal, in the
form or methyl mercury (CH3Hg+), is also problematic for living organisms This metal species
is the most toxic form of mercury because it has a high affinity to damage the central nervous
system, has high lipid solubility, and has the ability to reside in biological tissue for long periods
of time This compound can efficiently distribute throughout the body Unlike methyl mercury,
inorganic forms of mercury, such as HgCl, is less efficient at crossing biological membranes.
Inorganic forms of mercury primarily travel to visceral tissues, therefore the lower portions of
the body are generally affected by inorganic forms of mercury (Lovely, 2001) Mercuric forms
affect the distribution rate and toxicity level of this metal wilhin the body
The methods used to reduce mercury compounds from sites were also discussed in this study.
The reduction of Hg (II) to Hg (0) is a way to remove oxidized mercury compounds and to
reduce soluble mercury (Hg) from the atmosphere (Lovely, 2001) As stated previously,
mercury-resistant bacteria like Pseudomonas aergmosa can reduce Hg (II) to Hg (0). Reduction
of mercury from Hg (II) to Hg (0) allows a decrease in the mercury concentration from a
contaminated aquatic system Experts suggest that this method may serve as an effective m situ
remediation technique. Additionally, studies show that two-plasmid encoded membrane proteins
can assist with the reduction of cellular permeability to Hg (II) through a bacteria strain
Enterobacteria aerogenes (Lovely, 2001).
In the discussed case study, "Removal of Mercury from Chemical Wastewater by
Microorganisms in Technical Scale," the reduction of mercury Hg (II) to Hg (0) took place with
the use of mercury-resistant bacteria with a cytoplasmic mercuric enzyme that was encoded by a
mer A gene DNA sequences consist of operons and genes. Operons are groups of genes that
produce vital proteins for cells. There are two kinds of genes, structural and regulator genes, in
an operon Structural genes code for proteins and can form a single mRNA molecule during
transcription. Regulator genes code for proteins can regulate other genes. Today, the full or
partial DNA sequences of at least 10 mer operons are known (Lovely, 2001). The discovered
DNA sequences are derived from different gram-negative bacteria species. However, they
possess similar mer genes Studies show operons are not found in eukaryotes There are more
recognized mer operons in gram-negative bacteria than in gram-positive bacteria The presence
of mer genes have been identified by gene-probing isolated bacteria species from mercury
polluted and non-polluted environmental sample Research also shows mer gene expression can
occur in situ (Lovely, 2001) Moreover, studies are examining mer mRNA production and
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
mercury volatilization in environmental water samples. The mer mRNA is known to be affected
by microbial activity and not affected by bactena resistance Mercury resistant bacteria and mer
genes are important factors in the biocycling of mercury within the environment
The case study entitled "Microbial Reduction and Oxidation of Mercury m Fresh Water Lake"
examined the effects of microbial mercury oxidation and reduction processes to dissolved
gaseous mercury in freshwater lakes (Steven D. Siciliano, Nelson J. O'Driscoll, and DR.S,
2002). Studies show that decrease in dissolved gaseous mercury from lakes and rivers occurs
because of the invasion of elemental mercury in the environment. Bacteria that are often located
in freshwater lakes maintain an elemental level of mercury reductase activity that is able to
reduce low concentrations of mercury from Hg2+ to Hg°. It was discovered through the
experiment that microbial activity produces dissolved gaseous mercury and is affected by the
concentration of gaseous mercury.
These case studies highlight the methods used to effectively bioremediate mercury. Current
studies show that increasing the number of plasmids containing mer genes and using
permeabilized E coll cells that express mercuric reductase can facilitate the volatilization of
mercury Hg (0), which helps with the removal of mercury from aquatic systems (Lovely, 2001).
The re-engineering of the mer A. gene in the plant, Arabidopsis thaliana, created a mercury
volatizmg plant (Lovely, 2001) This method is useful from removing and immobilization of
toxic metals in plant biomass
Nevertheless, current approaches to bioremediating mercury rely upon microbial biosorption or
ion-exchange resins However, these strategies hold limitations because they lack sorption
specificity in the presence of other metal ions and can be influenced by pH and the presence of
metal ion-chelatmg agents. Biosorption of mercury (Hg2*) is enhanced with the expression of
mercuric ion transport proteins. The expression of mer T. and mer P. genes in Escherichia coh
can enhance Hg (II) accumulation (Lovely, 2001) Pseudomonas aeruginosa can further assist
with the biosorption of mercury (Lovely, 2001). The biosorption of mercury (Hg II) compounds
allow immobilization of mercury ions to occur, which is beneficial to the environment (Lovely,
2001) In addition, immobilization of mercury is better than volatilization of mercury because
studies show immobilization allows better revitalization of contaminated sites.
Implementation of bioremediation methods should be done with caution because many sites
contain variables, such as multiple metals, organic compounds, and organisms, that affect the
productivity of bioremediation approaches. Therefore remediation of contaminated sites usually
requires a combination of many different approaches Consequently bioremediation of mercury
is complex and requires great understanding of microbial mercuric reactions within different
environments
References
Agency for Toxic Substances and Disease Registry, ATSDR. 1999. hitp //www atsdr ccjcgoy.
Curtis D. Klaassen and John B. Watkins III Absorption, Distribution, and Excretion of
Toxicants. KarlK. Rozman Essentials of Toxicology. 2003.
Cynthia R Evanko, Ph.D, and David A. Dzombak, Ph D. Remediation of Metals-Contaminated
Soil and Groundwater. GWRTAC. October 1997. www awrlac ore
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Irene Wagner-Dobler, Harald von Canstein, Ymg Li, Kenneth N Timmis, and Wolf-Dieter
Deckwer. Removal of Mercury from Chemical Wastewater by Microorganisms in Technical
Scale Environmental Science. 2000 Volume 34
Marky Y. Vilensky, Brian Berkowitz, and Abraham Warshawsky: In situ Remediation of
Groundwater Contaminated by Heavy-and Transition-metal Ions by Selective Ion-Exchange
Methods Environmental Science Technology. 2002 36,1851-1855
Steven D Siciliano, Nelson J. O'Dnscoll, and D. R S Lean. Microbial Reduction and Oxidation
of Mercury in Freshwater Lakes. Environmental Science and Technology. 2002 Volume 36
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
BIBLIOGRAPHY AND ADDITIONAL READING
Agency for Toxic Substances and Disease Registry, ATSDR. 1999. hitp //www atsdr cdc gov.
Anushree Malik. Metal Bioremediation Through Growing Cells Environmental International
August 2003 Volume 30.
Anna Jensen-Spaulding, Michael L Shuler, and Leonard W Lion. Mobilization of Adsorbed
Copper and Lead From Naturally Aged Soil by Bacterial Extracellular Polymers.
Water Research. 2004. Volume 38, Issue 5.
Connor, Dave. Landm, Paul Mellor, Eric. O'Donovan, Christine. The Microbiology of In Situ
Bioremediation.
httpV/www cee.vt edu/program areas/environmental/teach/awpnmer/biorem html
Curtis D Klaassen and John B. Watkins III Absorption, Distribution, and Excretion of
Toxicants. KarlK Rozman Essentials of Toxicology. 2003
Cynthia R Evanko, Ph D, and David A Dzombak, Ph D. Remediation of Metals-Contaminated
Soil and Groundwater GWRTAC. October 1997. www gwrtac org
Derek R Lovely. Anaerobes to the Rescue. Science. 2001. Volume 293.
Haluk Beyenal and Zbigmew Lewandowski Dynamics of Lead Immobilization in Sulfate
Reducing Biofilms. Science Direct. June 2004. Volume 38, Issue 11
Irene Wagner-Dobler, Harald von Canstem, Ying Li, Kenneth N. Timmis, and Wolf-Dieter
Deckwer. Removal of Mercury from Chemical Wastewater by Microorganisms in Technical
Scale Environmental Science 2000 Volume 34.
Geoffrey M Gadd Microbial Influence on Metal Mobility and Application for Bioremediation
Science Direct. 2004.
Joanne M Santim, Lindsay I Sly, Roger D Schnagl, and Joan M. Macy A New
ChemolithoautotrophicArsenite-Oxidizmg Bacterium Isolated from a Gold Mine- Phylogenetic,
Physiological, and Preliminary Biochemical Studies. Applied and Environmental Microbiology.
2000 p. 92-97.
Jonathan R Lloyd and Derek R Lovely Microbial Detoxification of Metals and Radionuclides.
Current Opinion m Biotechnology 2001. 12.248-253.
Juerg Zobrist. Mobilization ofArsenile by Dissimilatory Reduction of Adsorbed Arsenate
Environmental Science Technology 2000. 34, 4747-4753.
Liesbet van Cauwenberghe and Dianes Roote, P G In situ Bioremediation GWRTAC. 1998
41
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
Marky Y Vilensky, Brian Berkowitz, and Abraham Warshawsky: In situ Remediation of
Groundwater Contaminated by Heavy-and Transition-metal Ions by Selective Ion-Exchange
Methods Environmental Science Technology. 2002 36, 1851-1855
Natural and Accelerated Bioremediation Research (NABIR) Program, Office of Biological and
Environmental Research, Office of Science, U S Department of Energy What is Bioremediation
2003. pp. 9.
National Research Council. Rittmann, Bruce, Alvarez-Cohen, Lisa, Bedient, B Philip, Brown,
A. Richard, Chapelle, H. Francis. In situ Bioremediation When does it work?, pg 13
Robert Rynk. Bioremediation with Cheese Whey. Journal of Composting and Organics
Recycling. 2000 45, 26
Steven D. Siciliano, Nelson J O'Dnscoll, and D R S. Lean. Microbial Reduction and Oxidation
of Mercury in Freshwater Lakes Environmental Science and Technology 2002 Volume 36.
USEPA Cleaning Up the Nation's Waste Sites. Markets and Technology Trends EPA 542-R-
04-015. 2004.
USEPA Office of Solid Waste and Emergency Response Use of Bioremediation at Superfiind
Sites. EPA542-R-01-019. 2001
USEPA Office of Solid Waste and Emergency Response About Superfiind
http //wwvv epa gov/superfund/aboui htm
W.L. Smith and G.M Gadd. Reduction and Precipitation ofChromate by Mixed Culture
Sulphate-reducing Bacterial Biofilms Journal of Applied Microbiology. 2000
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Bioremediation of Arsenic, Chromium, Lead, and Mercury
PERSONAL CONTACTS
Anne 0. Summers
Department of Microbiology
University of Georgia
527 Biological Sciences Building
Athens, GA 30602-2605
Phone 706-542-6140
Email. summers(a),ima edu
Diana A Blake
Tulane University
Xavier Center for Bioenvironmental Research
Tulane University Health Services Center
1430 Tulane Ave.
New Orleans, LA 70112
Phone 504-584-2478
Fax 504-584-2684
Email blake@tulane edu
Jonathan Istok
Department of Civil Engineering
Oregon State University
Apperson Hall 202
Corvalhs, OR 97331-4501
Phone:541-737-8547
E-mail jack istokffiprst edu
Yun A Gorby
Pacific Northwest National Laboratory
PO Box 999, MS P7-50
Richland, WA 99352
Phone-509-373-6177
Fax: 509-376-1321
Email vun gorbv@pnl gov
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