United States
Environmental Protection
Agency
Volume 9
Number 2 July 1987
oEPA NEWSLETTER
Quality
Assurance
CONTENTS
Memorial to Bob Kroner 2
Scientific/Technical Highlight 3
SW-846 3
Test Method Equivalency 3
QA 3
Environmental Monitoring and Support Laboratory-
Cincinnati (EMSL-Cincinnati) 4
Mailing List Verification 4
New Telephone Changes for the Office of the Director (OD)
Staff 4
Physical and Chemical Methods Branch 5
New Methods for the Support of the National Pesticide
Survey (NPS) 5
Methods Manuals for Organics in Drinking Water 5
Intergovernmental Personnel Act (IRA) Assignment 5
Automatic Sequential Sampling of Volatile Organics in
Water 5
Announcement 6
Method 200.1: Determination of Acid Soluble Metals 6
Method 200.11: Determination of Metals in Fish Tissue by
Inductively Coupled Plasma-Atomic Emission
Spectrometry 6
Multi-Laboratory Evaluation of a Broad Spectrum Gas
Chromatograph/Mass Spectrometer (GC/MS)
Method 7
Biological Methods Branch 7
Microbiology 7
Workshop on Drinking Water Quality: Current Trends
and Emerging Problems 7
Drinking Water Laboratory Certification Courses 8
Evaluation of the Hydrophobic Grid Membrane Filter (MF)
Procedure for Coliforms to be Published 8
Screening Test for Acceptability of MFs 8
Comparison of Media for the Isolation and Enumeration
of Staphylococcus aureus in Swimming Pool Water 8
Virology 9
Revision of Chapter of the USEPA Manual of Methods for
Virology EPA/600/4-84/013 9
Aquatic Biology 9
Draft Marine Rapid Chronic Toxicity Test Manual
Distributed 9
Report on the Taxonomy of Ceriodaphnia Available 9
Correction in Project Summary (EPA/600/S4-86/032) for
Ceriodaphnia Taxonomy Study 9
Meeting of the Biological Advisory Committee 10
Environmental Monitoring Systems Laboratory - Las Vegas, Nevada
(EMSL-Las Vegas) 10
Superfund Contract Laboratory Program (CLP) Reference
Material 10
Inorganic Reference Materials 10
Organic Reference Materials 10
UNLV-QAL Reference Materials 11
Headquarters
Office of Water - Washington, DC 11
Progress Resport on the Discharge Monitoring Reports (DMR)
QA Program 11
QA Support for Water and Wastewater Analyses 13
The USEPA Repository for Toxic and Hazardous Materials 18
How to Order Publications 27
QA Reference Books 27
Where to Write 28
Regional QA Coordinators 29
OA Officer Changes 30
Response Sheet 31
Mailing List Update 33
Mailing List Request Form for QA Newsletter 35
U.S. Environmental Protection Agency
Office of Research and Development
Environmental Monitoring and Support Laboratory
Cincinnati, Ohio 45268
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MEMORIAL TO BOB KRONER
Robert C. Kroner, affectionately known to us all as
Bob, died this past April. He was truly one of our pioneer
environmental research scientists, starting his career
at the U.S. Public Health Service (USPHS) research
station at the old Kilgore Mansion, and becoming one
of the original employees of the U.S. Environmental
Protection Agency (USEPA) when it was created in
1970. Bob was part of the original team that did
methods research in the basic measurement tech-
niques for municipal and industrial wastes. He was
one of the founders of the National Water Quality
Network (NWQN), a system of surface monitoring
stations, which is still in existence, providing valuable
water quality data to the national program. Most of
you probably remember him as Chief of our Physical
and Chemical Methods Branch, the position he held
at the time of his well-deserved retirement.
During his career. Bob authored numerous publica-
tions on the analytical chemistry of water and wastes.
He received the Award of Merit in 1975, the highest
award presented by American Society for Testing and
Materials (ASTM), which also gave him the Max Hecht
award in 1984 for his outstanding contributions to the
study of water chemistry. A man of many interests,
he combined his love of Cincinnati and flair for
photography into a travelling slide show for the
Cincinnati Historical Society.
Bob was a good friend and mentor to many of us.
He made significant contributions to the field of
environmental monitoring that will serve us for years
to come. We'll miss him.
(Robert Booth, FTS: 684-7301; COML: 513-569-7301)
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Scientific/Technical Highlight
Office of Solid Waste (OSW) - Washington, DC
SW-846 Methods
The Technical Assessment Branch, Characterization
and Assessment Division, of OSW, is the focal point
for expertise in all aspects of chemistry, testing
methodology, quality assurance (QA), risk assessment
and toxicology. The Methods Section of this Branch,
headed by David Friedman, directs OSW's program to
develop and promulgate test methods for identifying
hazardous wastes and for determining the properties
of wastes. It is also responsible for implementing the
Resource Conservation and Recovery Act (RCRA) QA
program.
When the RCRA regulations were proposed in 1978,
the USEPA wanted to inform the regulated community
about test methods which were acceptable for
determining compliance with the regulations. A
methods manual was made available through the public
printing agencies of the government. The first edition:
Test Methods for Evaluating Solid Waste, Physical/
Chemical Methods (SW-846), was published in 1980.
Its purpose was to present the state of the art in routine
analytical testing, adapted for the RCRA program SW-
846 methods. To keep current, it was updated yearly.
The second edition was published in 1982 and
updated twice to keep pace with the state of the art
and the growing RCRA program. In 1984, OSW
proposed to make it mandatory for all RCRA testing
and to include a number of new methods. Comments
on this proposal indicated that the second edition
needed modifications and more supporting data before
it became mandatory.OSW responded with method
validation studies to gather supporting data .The text
of the methods was edited for clarity and a standard
format was adopted. SW-846 was modified, to the
extent possible, to make it consistent with the
Comprehensive Environmental Response Compensa-
tion and Liability Act (CERCLA) Contract Laboratory
Program (CLP) methods and methods from the Office
of Water.
The third edition was published this past April and
contains a revised Quality Control (QC) chapter. OSW
is placing emphasis on QC so the quality of data
produced by the methods is known. OSW will propose
to make this QC, contained in Chapter One, mandatory
for all RCRA testing and to sustitute the third edition
for the second edition in the regulations. OSW will
study the comments made in response to this proposal
and determine whether or not to propose to make the
third edition mandatory for all RCRA testing. OSW will
update the third edition annually. The manual will be
modified in response to public comment and RCRA
requirements.
The third edition is available from the Superintendent
of Documents, U.S. Government Printing Office,
Washington, DC 20402, on a subscription basis. The
subscription includes both the third edition of the
manual and a number of future updates. The cost is
$110.00 per subscription for domestic mailing
($137.50 if mailed to a foreign address). The document
number is 955-001-00000-1. The second edition of
SW-846 is still referenced in several sections of the
RCRA regulations, copies will be available through the
National Technical Information Service (NTIS), U.S.
Department of Commerce, 5285 Port Royal Road,
Springfield, VA 22161, COML: 703-487-4650.
Test Method Equivalency
The RCRA regulations (40 CFR 260.20) establish
procedures by which persons may petition USEPA to
approve the use of alternative or equivalent testing
procedures when conducting testing under RCRA. A
manual has been prepared to provide guidance for
persons submitting a test method petitition. The
document explains, in detail, the information a test
method equivalency petition must include. Copies are
available from the National Technical Information
Service (NTIS), U.S. Department of Commerce, 5285
Port Royal Road, Springfield, VA 22161, COML: 703-
487-4650. The cost is $18.95 per paper copy and $6.50
per microfiche. The document number is PB-87-
178349.
QA
The primary goals of the OSW Quality Assurance
Program (QAP) are two-fold. The first is to insure that
the data collected is sufficient to permit us to answer
the question for which it is being gathered. The second
is to insure that all test data will be of known quality.
Ongoing QA efforts in OSW are:
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1. Conduct Laboratory Evaluation Program (LEP)
(Supported by ORD; EMSL-Cincinnati and EMSL-
Las Vegas). This program involves about 45
Regional, State and OSW contractor laboratories.
2. Review and approve all plans for data gathering
projects in OSW prior to start of the activity.
3. Issue memoranda "Notes on RCRA Methods and
QA Activities" each 6-8 weeks. The memorandum
series is designed to help the Regions and States
keep abreast of RCRA activities in methods
development, QA, hazardous waste identification
characteristics and to solicit ideas and participa-
tion by Regional and State personnel in OSW
workgroups.
4. Perform systems audits on laboratories under
contract to OSW.
5. Prepare manuals or guidance material as needed.
6. Participate in planning and operating the annual
symposium, "Solid Waste Testing and QA". (The
most recent symposium was held July 13-14,
1987, in Washington, DC).
7. Review and rate the QA section in all proposals
for OSW contracts.
8. Procure samples and oversee operation of
repository of hazardous wastes.
9. Oversee a QA Work Group which supports the
efforts of OSW in the QA area. Any questions
on the above QA program may be directed to
Florence Richardson, OSW's QAO.
(Denise Zabinski, FTS: 202-382-7458; COML: 202-
382-7458)
Environmental Monitoring and Support Laboratory - Cincinnati
(EMSL-Cincinnnati)
Mailing List Verification
In compliance with prior notice and government
requirements to perform an annual review of mailing
list, the Mailing List Update page of this Newsletter
(next to the last page) must be returned to the writer
by August 31. 1987. A new list will be established
in September. Continue to send information by
electronic mail, through use of magnafax (FTS: 684-
7274 and 684-7276), or mail articles to: Betty Thomas,
Publications Assistant, EMSL-Cincinnati, 26 West St.
Clair Street, Cincinnati, Ohio 45268.
New Telephone Changes for the Office of the Director (OD) Staff
Effective immediately, the OD staff has made some telephone changes. The new telephone changes for the
OD staff are as follow:
Director's Office
Robert Booth
Beverly Halbe
Budget/Fiscal Office
FTS: 684-7301
COML: 513-569-7301
Terri Firestone
Debra Lang
FTS: 684-7305
COML: 513-569-7305
Deputy Director's Office
Thomas Clark
Joan Lobitz
FTS: 684-7303
COML: 513-569-7303
Equivalency Program
Terence Grady
FTS: 684-7328
COML: 513-569-7328
Publications Office
Betty Thomas
Jerry Bivens
FTS: 684-7302
COML: 513-569-7302
Science Advisor's Office
Robert Graves
FTS: 684-7315
COML: 513-569-7315
Administration Office
Kathie Fieler
Shirley Graden
FTS: 684-7304
COML: 513-569-7304
QA Management Staff Liaison
Daniel Bender
FTS: 684-7351
COML: 513-569-7351
(Betty Thomas, FTS: 684-7302; COML: 513-569-7302)
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Physical and Chemical Methods Branch
New Methods for the Support of the National Pesticide Survey (NFS)
Six new methods have been developed through
contract with Battelle-Columbus Laboratories for the
determination of a total of over 100 pesticides and
pesticide metabolites. These methods are currently
being used by Battelle and USEPA laboratories in the
NPS pilot study. A comprehensive review of these
method write-ups by USEPA, State and private
concerns will be conducted. The methods should be
finalized and available by October, 1987. They will be
used for the NPS that is scheduled to begin this fall.
Also, they may be proposed to support future regu-
lations developed by the ODW or the Office of Pesticide
Programs (OPP) of USEPA.
The six methods include five broad-spectrum
methods: [three capillary column gas chromatographic
(GC) methods and two high performance liquid
chromatographic (HPLC) methods] and one single-
analyte method [a GC method for measuring ethylene
thiourea]: neutral halogenated pesticides are deter-
mined by methylene chloride extraction and GC/
electron capture detector; halogenated acids are
determined by ethyl ether extraction, methylation,
column cleanup, and GC/electron capture detector;
nitrogen and/or phosphorus containing pesticides are
determined by methylene chloride extraction and GC/
nitrogen-phosphorus detector; non-volatile, nonpolar
analytes are determined by methylene chloride
extraction and HPLC/ultraviolet detector; non-volatile,
, nonpolar analytes are measured by direct liquid
injection, HPLC/post-column reaction and a fluores-
cence detector; and ethylene thiourea is measured by
concentrating on a solid adsorption column, methylene
chloride elution and GC/nitrogen-phosphorus detector.
(Robert Graves, FTS: 684-7315; COML: 513-569-7315)
Methods Manuals for Organics in Drinking Water
Six methods prepared in support of the final rule
on volatile organic compounds (VOC) in drinking water
are now available from EMSL-Cincinnati. These
methods have been edited in response to public
comments received on the proposed rule of November
13, 1985, and include the two capillary column purge
and trap methods described in the February, 1987
edition of this Newsletter.
The methods are being typeset and punched for
insertion into a three-ring binder that will contain all
of the methods related to the measurement of organics
in water to meet the requirements of the Safe Drinking
Water Act.
(James Longbottom, FTS: 684-7308; COML: 5I3-569-
7308}
Intergovernmental Personnel Act (IPA) Assignment
Dr. Fred Kawahara of the EMSL-Cincinnati has
accepted an IPA assignment with the National Bureau
of Standards (NBS) for a six-month period. The research
that is currently being conducted at NBS is compli-
mentary to the work of Dr. Kawahara's on the coating
of fiber optics with silane polymers to sensitize the
fiber optics to the presence of trace amounts of
aromatics. The NBS is also developing an analytical
method for aromatics by observing the reaction of
aromatics with antigens coated on glass beads.
(Joseph Roesler, FTS: 684-7286; COML: 513-569-
7286)
Automatic Sequential Sampling of Volatile Organics in Water
Dr. James Shou-Yien Ho of the EMSL-Cincinnati has
'successfully completed his research on the design,
construction, and evaluation of two automatic bottle
sealing mechanisms for the collection of volatile
organic compounds in water. This information is
published in the June 1987 issue of the Water
Resources Bulletin, American Water Resources
Association, Volume 23, No. 3. Dr. Ho compared
samples of volatile organics that were manually
collected in uncapped bottles and in bottles capped with
the automatic bottle sealing mechanisms. After 24
hours' storage, significant losses of volatile compounds
from uncapped bottles occurred even if the samples
were stored at 4°C, but not for the samples capped
with the automatic bottle sealing mechanisms. The
mechanism for sealing the sample bottles was
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integrated into a commercial sampler with minor
modifications to the sampler. The sampling system was
then evaluated under controlled conditions to simulate
actual field sampling. The results showed that the
system was suitable for taking sequential discrete
water samples automatically for 24 hours without
significant losses of volatile organic compounds.
(Joseph Roesler. FTS: 684-7286; COML: 513-569-
7286)
Announcement
EMSL-Cincinnati is considering a recommendation
to remove the Brucine Method (352.1) for nitrate
measurement from the list of approved drinking water
methods. It is felt that the method's extreme depend-
ence upon temperature creates considerable possibility
of error. We would like to have written comments from
any readers who may be using this method. Write to
John Pfaff, Research Chemist, Inorganic Analyses
Section. Physical and Chemical Methods Branch,
EMSL-Cincinnati, 26 West St. Clair, Cincinnati, Ohio
45268.
In addition to the recommendation for removal of
brucine, EMSL-Cincinnati is also considering recom-
mending the removal of the Atomic Absorption,
Furnace Technique for Sodium. Method 273.2, from
the list of approved drinking water methods. Any
comments should be addressed to John Pfaff at address
given above.
(John Pfaff, FTS: 684-7313; COML: 513-569-7313)
Method 200.1: Determination of Acid Soluble Metals
To measure and define a more realistic impact of
metal toxicity on the aquatic environment, the Agency
has established the concept of "acid-soluble" metal.
The term refers to the solubilized metal fraction of a
sample that will pass through a 0.45 // membrane filter
after acidification with nitric acid to a pH between 1.5
to 2.0. The "acid-soluble" metal measurement is
believed to be less rigorous and a more scientifically
correct basis upon which to establish ambient water
quality criteria than "total" or "total-recoverable" metal
analyses. Method 200.1 describes the sample prep-
aration procedure for the determination of six acid-
soluble metals (As, Cd, Cr, Cu, Pb and Hg) by atomic
spectroscopy. However, the method does not distin-
guish oxidation states or organometallic speciation. The
method has been evaluated by being subjected to
limited single laboratory ruggedness testing covering
the analyses of 10 metals. Copies of the draft report
that discusses the single laboratory evaluation along
with copies of Method 200.1 are available from EMSL-
Cincinnati by calling Ms. Louise Hoffman, FTS: 684-
7586; COML: 513-569-7586. Comments and sugges-
tions concerning the method are welcome.
(Theodore Martin, FTS: 684-7312; COML: 513-569-
7312)
Method 200.11: Determination of Metals in Fish Tissue by
Inductively Coupled Plasma-Atomic Emission Spectrometry
The present dry ashing interim method recom-
mended for whole fish analyses has been criticized as
being time consuming and subject to contamination
because of the number and length of operative steps
involved in the procedure. In response to this criticism
and for a more reliable procedure. Method 200.11 was
developed. In Method 200.11 a 1 to 2 gram aliquot
of fresh tissue is placed in labeled, pre-weighed
polysulfone Oak Ridge type centrifuge tube for
transport, storage and sample processing. At the time
of analysis, the tissue is dissociated using tetrame-
thylammonium hydroxide, mild heating and vortex
mixing. The resulting colloidal suspension is cooled,
partially oxidized with hydrogen peroxide, and the
metals are finally solubilized in nitric acid with
additional heating. After solubilization, the volume of
the acid solution is diluted with deionized, distilled
water to a uniform weight/volume ratio of 1 gram fish
tissue per 10mL of solution. The solution is then mixed,
centrifuged and analyzed directly from the centrifuge
tube by inductively coupled plasma-atomic emission
spectrometry. The analytical results are reported in
concentration of jug/gram wet tissue weight. Method
200.11 is applicable to the analyses of aluminum,
antimony, arsenic, beryllium, cadmium, calcium,
chromium, copper, iron, lead, magnesium, nickel,
phosphorus, selenium, sodium, thallium and zinc.
Included in the method description are typical method
detection limits along with single laboratory precision
and accuracy data determined using bluegiil fillet
tissue. Copies of the method are available from EMSL-
Cincinnati by calling Ms. Louise Hoffman, FTS: 684-
7586; COML: 513-569-7586. Comments and sugges-
tions concerning the method are welcome.
(Theodore Martin, FTS: 684-7312; COML: 513-569-
7312)
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Multi-Laboratory Evaluation of a Broad Spectrum
Gas Chromatograph/Mass Spectrometer (GC/MS) Method
The EMSL-Cincinnati laboratory is assisting the
Office of Emergency and Remedial Response (OERR)
with a seven-laboratory test of a method for the
identification and measurement of a broad range of
organic compounds. The purpose of the test is to
demonstrate that it is not only feasible, but also cost-
effective to identify and measure polychlorinated
biphenyls (PCBs), chlorinated hydrocarbon pesticides,
a wide range of base-neutrals, and phenols with a
single streamlined procedure. The method is designed
for real-world, highly contaminated water and soil
samples from abandoned hazardous chemical waste
sites where compounds are ordinarily present in
concentrations above one part per billion. The test
method uses an MS to detect sample components as
they elute from a fused silica capillary column in a
GC. The MS is operated in the standard full-range mass
spectrum mode.
Water and soil samples will be extracted in the test
of the method using procedures similar to those
currently used by OERR in its contract laboratory
program. Changes to these procedures for this test
were designed to improve method performance and
include a change in the pH adjustments prior to
extraction of water samples and improved gel permea-
tion chromatography procedures for soil sample
extracts. The PCBs will be measured using the approach
of Method 680; that is, the identification and measure-
ment of PCB components by level of chlorination.
Groups of PCB components at each level of chlorination
are called isomer groups, and total PCBs are measured
by summing the quantities of isomer groups.
In the seven-laboratory test, automated procedures
will be used to identify PCBs by level of chlorination,
identify pesticides, carry out the calculations required
to produce the final results, and generate reports on
magnetic media (diskettes) for submission to USEPA.
The automated procedures are contained in a set of
computer programs (software) developed by EMSL-
Cincinnati. These programs execute on the same
computer used for the GC/MS data acquisition (or a
personal computer) and their use substantially reduces
the time and effort required to obtain the final results.
Accuracy and precision of the identifications and
measurements are improved by the elimination of the
need to inspect numerous mass spectra and carry out
extensive calculations.
• Results of the seven-laboratory test will be presented
in the form of a detailed written report in November
1987.
(William Budde, FTS: 684-7309; COML: 5I3-569-7309)
Biological Methods Branch
Microbiology
Workshop on Drinking Water Quality: Current Trends and Emerging Problems
A workshop addressing recent concerns in drinking
water quality was sponsored by the Office of Continuing
Education, American Society for Microbiology (ASM),
at the Valley Forge Regional Meeting held May 8,1987
in King of Prussia, Pennsylvania. Several microbiol-
ogists from USEPA, the Centers for Disease Control
(CDC), and academic institutions participated. The
workshop was moderated by Robert Bordner and
included sessions on "Biofilm Occurrence in Water
Pipes," Edwin E. Geldreich, Water Engineering
Research Laboratory - Cincinnati; "Evaluation of
Alternative Coliform Membrane Filter Media for
Drinking Water," Robert Bordner, Environmental
Monitoring and Support Laboratory - Cincinnati;
"Potential Problems in Drinking Water: Giardia and
Cryptosporidium," Frank W. Schaefer, Toxicology and
Microbiology Division - Cincinnati, Health Effects
Research Laboratory; "Association of Heterotrophs and
Nosocomial Infections," Anita K. Highsmith, Nosocom-
ial Infections Laboratory Branch, Center for Infectious
Diseases, CDC, Atlanta, Georgia; "Application of
Conventional Coliform Data to the Frequency of
Occurrence Concept," Wesley 0. Pipes, and
"Occurrence and Significance of Fungi in Drinking
Water," William Rosenzweig, Drexel University,
Philadelphia; and "Pros and Cons of Concentration-
time (C-t) Tables in Disinfection Treatment," Vincent
Olivier!, Johns Hopkins University, Baltimore, Mary-
land. Requests for more information should be
addressed to the individual speakers.
(Robert Bordner, FTS: 684-7319; COML: 513-569-
7319)
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Drinking Water Laboratory Certification Courses
EMSL-Cincinnati held three Drinking Water Labor-
atory Certification courses in June at the Andrew W.
Breidenbach Environmental Research Center
(AWBERC), Cincinnati, for Laboratory Certification
Officers from USEPA regions and the states in support
of the Agency's QA program. A Microbiology course
was held during the week of June 8, and Chemistry
courses were held during the weeks of June 15 and
22. All three courses were filled to capacity. The course
contents included the legislative mandate and devel-
opment of the national certification program, current
analytical methodology and QA procedures, essential
background information, and procedures for on-site
laboratory visits.
(Robert Bordner, FTS: 684-7319; COML: 513-569-
7319 and Jack Pfaff, FTS: 684-7312; COML: 513-569-
7312)
Evaluation of the Hydrophobia Grid Membrane Filter (MF)
Procedure for Coliforms to be Published
EMSL-Cincinnati compared the hydrophobic grid
membrane filter (HGMF) to the standard MF and most
probable number (MPN) procedures for the detection
and enumeration of coliforms in nonchlorinated
wastewaters, and polluted surface and dosed drinking
waters. Recoveries of fecal coliforms were significantly
higher with the MPN method than with the filtration
methods only for the nonchlorinated domestic waste-
waters. No significant difference was observed in
recoveries of fecal and total coliforms with either MFs
or HGMFs. Total coliform results obtained with HGMFs
having greater than 100 positive grid cells were
significantly more precise than estimates obtained with
the standard MF method only for polluted surface
waters. The results of this study are scheduled to be
published in the May 1987 issue of Applied and
Environmental Microbiology.
(Audrey McDaniels, FTS 684-7332; COML: 513-569-
7332)
Screening Test for Acceptability of MFs
The accurate recovery of bacteria from water samples
using the MF method depends on the quality of the
filters used. Any defects in the filters due to changes
in materials, manufacturing processes, storage
conditions, or degree of quality control (QC) may result
in an increase or decrease in the recovery of
microorganisms. A study of MF irregularities, such as
partial or complete inhibition of microbial growth at
the gridlines, abnormal spreading of colonies, non-
wetting areas, growth in and along the gridlines, poor
colony sheen development and formation of a metallic
sheen on the MF surface on Endo agar, decreased
recovery, and various combinations of these defects,
found on several lots of MFs during routine QC
procedures has resulted in the development of a
sensitive screening test for MF acceptability. The test
was described in an oral presentation entitled "A New
Screening Test to Determine the Acceptability of 0.45
fjm Membrane Filters for the Analysis of Water,"
presented at the Annual Meeting of the American
Society for Microbiology (ASM) held in Atlanta, Georgia,
March 4, 1987.
(Kristen Brenner, FTS: 684-7317; COML: 513-569-
7317)
Comparison of Media for the Isolation and Enumeration of
Staphylococcus aureus in Swimming Pool Water
A paper was presented at the poster session of the
American Society for Microbiology (ASM) annual
meeting on the results of research comparing Baird-
Parker (BP) agar. Vogel-Johnson (VJ) agar, and M-
Staphylococcus (MS) broth for the isolation and
enumeration of S. aureus in swimming pool water
using the membrane filter method.
Alternatively, recovery of S. aureus with BP agar,
VJ agar, and MS broth ranged from 87-117%, 25-89%,
and 52-96% respectively, compared to recovery on a
nonselective medium. In swimming pool comparison
studies statistically significant higher recoveries of
staphyiococci were obtained with BP agar. VJ agar
and BP agar demonstrated poor specificity for S. aureus,
a
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but were specific and selective for staphylococci. When
isolates were speciated, the accepted description of
"typical" colony morphology with either VJ or BP agar
correlated poorly with identification as S. aureus.
Neither VJ nor BP agar was found acceptable for
enumeration of S. aureus, but both were acceptable
for total staphylococci. MS broth was not sufficiently
differential or selective for quantitative recovery of
staphylococci or S. aureus. Copies of the poster are
available upon request.
(Terry Covert, FTS: 684-7318; COML: 513-569-7318)
Virology
Revision of Chapter of the USEPA Manual of Methods for Virology,
EPA/600/4-84/013
The revised Chapter, "Cell Culture Preparation and
Maintenance," EPA/600/4-84/013(R9). is available
for distribution. This chapter and the soon to be
completed chapter, "Cell Culture Procedures for Virus
Assay," will replace the current description of cell
culture procedures in the manual. These new chapters
will be designated as 9 and 10, respectively. The
chapter on "Virus Plaque Confirmation Procedure"
which was Chapter 10, will be renumbered as Chapter
11, and the current Chapter 11 ("Identification of
Enteroviruses") will be renumbered as Chapter 12.
These chapter number changes will be instituted in
1988, when the chapters are reissued.
(Robert Safferman, FTS: 684-7334; COML: 513-569-
7334)
Aquatic Biology
Draft Marine Rapid Chronic Toxicity Test Manual Distributed
A draft of the methods manual, "Short-term Methods
for Measuring the Chronic Toxicity of Effluents and
Surface Waters to Marine and Estuarine Organisms,"
was distributed for review by the Bioassay Subcom-
mittee of the EMSL-Cincinnati Biological Advisory
Committee, USEPA Headquarters and regional staff,
other Federal agencies, state and interstate water
pollution control programs, environmental protection
groups, trade associations, major industries, consulting
firms, academic institutions engaged in aquatic
toxicology research and other interested parties in the
private sector. The manual describes short-term (one-
hour to nine-days) methods for estimating the chronic
toxicity of effluents and receiving waters to two marine
fish, two invertebrates, and an alga. Also included are
guidelines on laboratory safety, QA, facilities and
equipment, dilution water, effluent sampling and
holding, data analysis, report preparation, and
organism culturing and handling. Listings of computer
programs for Dunnett's Procedure and Probit Analysis
are provided in the Appendix.
(William Horning, FTS: 778-8350; COML: 513-527-
8350)
Report on the Taxonomy of Ceriodaphnia Available
The report, "Taxonomy of Ceriodaphnia (Crustacea:
Cladocera) in U.S. Environmental Protection Agency
Cultures," EPA/600/S4-86/032, by Dorothy B.
Berner, Temple University, can be obtained by
contacting ORD Publications, CERI, U.S. Environmental
Protection Agency, Cincinnati, Ohio, 45268. Cerio-
daphnia is an important toxicity test organism in the
Agency's new Water Quality Based Approach to the
control of toxic discharges in the Discharge Permit
Program. Correct identification of the test organisms
is important in establishing the validity of the test
results.
(William Horning, FTS: 778-8350; COML: 513-527-
8350)
Correction in Project Summary (EPA/600/S4-86/032} for Ceriodaphnia Taxonomy Study
The principal investigator (Dr. Berner) has indicated
that the last sentence in the "Conclusions" of the
recently distributed Project Summary (EPA/600/S4-
86/032) should read as follows: "It is likely, however.
-------
that other species of Ceriodaphnia exist that have a
heavy, fine-toothed central pecten on the claw similar
to that of dub/a. Therefore, that character alone should
not be used to identify animals found in natural
populations." For further information on Ceriodaphnia
taxonomy contact Dr. Berner. Biology Department,
Temple University, Philadelphia, PA 19122.
(William Horning, FTS: 778-8350; COML: 513-527-
8350)
Meeting of the Biological Advisory Committee
The Bioassay Subcommittee of the EMSL-Cincinnati
sponsored Biological Advisory Committee met in
Cincinnati, May 13-15. The meeting was attended by
approximately 25 members, representing regional
programs, ORD laboratories, and headquarters pro-
gram offices. Subjects discussed included: the
proposed revisions in the freshwater short-term
chronic toxicity test manual (EPA/600/4-85/014); the
draft marine short-term chronic toxicity test methods;
toxicity laboratory certification; fractionation of toxic
chemicals in effluents; the need for standardized
sediment toxicity test methods; biological assessment
protocols for superfund sites; revision of the (1973)
biological field and laboratory methods manual (EPA/
600/4-73/OOI); and biological assessments mandated
by the Water Quality Act of 1987.
(William Horning, FTS: 778-8350; COML: 513-527-
8350)
Environmental Monitoring Systems Laboratory - Las Vegas, Nevada
(EMSL-Las Vegas)
Superfund Contract Laboratory Program (CLP) Reference Material
The University of Nevada, Las Vegas Quality
Assurance Laboratory (UNLV-QAL) produces several
inorganic and organic reference materials (RMs) in
support of the Superfund Contract Laboratory Program.
The USEPA Regional personnel and Remedial Field
Investigation Teams (REM/FITs) may request these
materials through: Dr. Larry C. Butler, USEPA, EMSL-
Las Vegas, QA Research Branch, Post Office Box
15027, Las Vegas, NV 89II4-5027 (FTS: 545-5027;
COML: 702-798-2114).
Inorganic Reference Materials
Reference Materials suitable for inorganic analyses
presently include a natural ground rock matrix from
a local mine containing 23 elements. It is available
for use as a Lab Control Sample. Aqueous samples
include Initial Calibration Verification (ICV) Solutions
1-5, which can also be used as aqueous Lab Control
Samples, and an inductively coupled plasma (ICP)
Interference Check Sample. ICV-1 contains 18
elements; ICV-2 contains arsenic and selenium, ICV-
3 contains antimony; and ICV-4 contains lead.
cadmium, thallium, and silver; and ICV-5 contains
mercury. The ICP Interference Check Sample is a set
of two solutions containing 4 interferences and 13
analytes.
These materials have all been certified in interlab
studies. Instruction sheets for use of the materials as
well as qualitative and quantitative information are
included with each material requested. Materials
presently available are listed below.
Organic Reference Materials
The UNLV-QAL also produces several dioxin Perform-
ance Evaluation Materials (PEMs). These PEMs
presently consist of various fortified and unfortified
sands. (Fortified is defined as having analytes and/
or interfering compounds added). Dioxin PEMS are
available fortified at concentrations appropriate for low
10
-------
resolution mass spectrometry analysis parts per billion
(ppb). Blanks with and without interferences exist for
ppb and parts per trillion (ppt) levels of analysis.
Included in a dioxin PEM shipment is a listing of
nominal analytical values for each container in the
shipment and a listing of the PEM Sample Numbers.
The nominal value is an approximation of the analytical
value obtained in a single lab study. Dioxin PEMS
presently available are listed below.
The UNLV-QAL is planning to develop ppb and ppt
tetra-, penta-, hexa-, hepta-, and octachlorinated
dibenzo-p-dioxin (PCDD) and dibenzo-f urans (PCDF) soil
PEMs to support Method 8280 and a high resolution
mass spectrometry version of the method (8290). PEMS
composed of real matrices with all of their attendant
interferences are being developed now. Dioxin water
PEMs are not available; however, development is in
the planning stage.
UNLV-QAL Reference Materials
Inorganic Materials:
Interference Check Sample 0387; Laboratory Control
Sample: Initial Calibration Verification (ICV) Solutions
1-5.
Organic Materials:
Dioxin Materials: Fortified Kiln Ash; Fortified XAD
Resin; Fortified Filter Paper; Fortified Florisil; Fortified
ppb PEMs; Fortified PEM Blanks; PEM Blanks (ppb and
ppt). Requests, questions, and comments should be
addressed to Dr. Butler at the address and phone
number above.
(Amy Smiecinski, FTS: 545-3149; COML: 702-798-
3119)
Headquarters
Office of Water - Washington, DC
Progress Report on the Discharge Monitoring Reports (DMR) QA Program
Through EMSL-Cincinnati, Office of Water Enforce-
ment and Permits (OWEP) has been conducting a QA
program to assure the quality of DMR from the 7500
major National Pollutant Discharge Elimination System
(NPDES) permittees. Since 1980, six national studies
have been completed.
Major permittees are sent performance evaluation
(PE) samples containing constituents normally found
in industrial and municipal wastewaters. The samples
are to be analyzed with the method normally used to
generate the data submitted in DMRs. Responding
permittees subsequently receive an evaluation of their
data, and where necessary, are given guidance for
checking error sources.
The program has provided valuable data in assessing
the quality of self-monitoring data. Data quality has
steadily improved (as illustrated in Figures 1 and 2).
This program also enables the tracking of improve-
ments by permittee category, identifying the sources
of error, and improving the efficiency of NPDES
compliance monitoring.
Innovations for Study 6 include the following:
1. Effluent limit information from the Permit
Compliance System (PCS) has been used to
evaluate concentration ranges for the 29 analytes.
The results were used to set suitable concentra-
tion ranges for PE samples for the next study.
2. Performance can now be evaluated by method
code for each of the analytes. This capability
allows for assessing the frequency of use and also
comparing the effectiveness of different analytical
methods.
3. A code has been added for identifying the type
of laboratories used by permittees. This code
enables more effective follow-up of permittees
using commercial laboratories.
11
-------
100
90
80-
70
60
M
-------
QA SUPPORT FOR WATER AND WASTEWATER ANALYSES
EMSL-CINCINNATI
Absolute Limit on Numbers of Quality Control (QC) and
Performance Evaluation (PE) Samples
The anticipated initiation of a user-free program in To prevent a serious loss of sample inventories until
the U.S. Environmental Protection Agency (USEPA) has the final decision is made on user-fees, and mech-
caused a significant increase in requests from regional, anisms are in place for distribution, only a single set
state, and local laboratories, for large numbers of QC of samples of a type will be distributed to requesters,
samples or PE samples (outside of the Agency's formal as of May 6, 1987. There will be no exceptions.
studies).
Quality Control (QC) Sample Program
Single Level QC Sample Series
To increase efficiency and economy in the prepa- specific QC sample concentration for analytes, the
ration and distribution of QC samples, new or re-made concentration of the QC sample will be so set. For other
series are being prepared at one concentration/ analytes, a mid-range concentration will be provided.
analyte. For USEPA methods which specify use of a
Replacement QC Series
Aromatic Purgeables (Method 602), Halogenated contain Halogenated Purgeables. VOC I through VII
Purgeables—I and II (Method 601), Volatile Organics replace all of the Purgeables, seven mentioned above.
(Method 601 and 602), and GC/MS Purgeables I Polychiorinated Biphenyls (PCBs) in Fish, PCB Aroclor
through IV (Method 624) have been depleted and are 1262, Mercury (available in WP and WS Trace Metals),
replaced by the new VOC samples. Series I through and Temik and Municipal Digested Sludge have been
III contain Aromatic Purgeables and Series IV and VII depleted.
Availability of PCBs in Sediment QC Samples
A sediment sample containing both PCB Aroclor sediment. Reference values and standard deviations
1242 and PCB Aroclor 1254 is available. Each sample were generated by the National Bureau of Standards
bottle contains 50 grams of dried homogeneous (NBS).
Notice
As an economy measure, QC samples are now sent own QC activities. They are not intended to replace
by the least expensive means which may be United the standards, check sample, blind samples or
Parcel Service (UPS). Therefore, street addresses must replicates incorporated into each analytical run as part
be provided. of the laboratory's QC program.
The QC Sample series are intended for periodic use There is no certification or other formal evaluative
(quarterly) as independent checks on each laboratory's function resulting from the use of QC samples and data
13
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return is not expected. The QC Sample Program covers
the ambient water quality, drinking water, water
pollution, priority pollutant, hazardous, and toxic waste
programs for chemical, biological, and microbiological
analytes. Most samples are prepared as concentrates
in water or organic solvent sealed in glass ampuls.
Instructions are provided for dilution of samples to
volume with water or wastewater prior to analysis. The
following samples are available now:
DEMAND ANALYSES
EPA/API STANDARD
REFERENCE OILS
QC Samples for Water Quality Analyses
BOD, COD. and TOO
Arabian Light Crude Oil, Prudhoe Bay Crude Oil, South Louisiana Crude
Oil, No. 2 Fuel Oil (high aromatics), and No. 6 Fuel Oil (high viscosity/
Bunker C (laboratory must request specific oil).
LINEAR ALKYLA TE SULFONA TE
MINERAL/PHYSICAL ANALYSES
NONIONIC SURFACTANT
(CTAS TEST) STANDARD
NUTRIENTS
OIL AND GREASE
PESTICIDES IN FISH
PHENOLS, TOTAL (4AAP Method)
POLYCHLORINATED BIPHENYLS
(PCBs) IN OILS
POLYCHLORINATED BIPHENYLS
(PCBs) IN SEDIMENTS
SUSPENDED SOLIDS
TRACE METALS - WP I
TRACE METALS - WPII
TRACE METALS - WP HI
TRACE METALS IN FISH
LAS, the anionic surfactant standard for the MB AS Test
sodium, potassium, calcium, magnesium, pH, sulfate, chloride, fluoride,
alkalinity/acidity, total hardness, total dissolved solids, and specific
conductance.
Reference Nonionic Surfactant, C,
Standard Methods Method 512 C
n
nitrate-JV, ammonia-N, Kjeldahl-N, orthophosphate, and total P
analyzable by IR and gravimetrically
toxaphene. DDD, DDE. and DDT
total phenols in water
Aroclor 1016, 1242, 1254, and 1260 in transformer, hydraulic, and
capacitor oils, (specify Aroclor and oil)
Aroclor 1242 and 1254 in sediment
non-filterable, volatile and total filterable residue
aluminum, arsenic, beryllium, cadmium, chromium, cobalt, copper,
iron. lead, manganese, mercury, nickel, selenium, vanadium, and zinc
antimony, silver, and thallium
barium, calcium, potassium, sodium, magnesium, and molybdenum
arsenic, cadmium, chromium, copper, lead, mercury, nickel, selenium,
and zinc
QC Samples for Priority Pollutants/Hazardous Wastes/Toxic Chemicals
n-ALKANES
CHLORINATED HYDROCARBONS
(Method 612)
dodecane, eicosane, heptadecane, hexacosane, tetradecane, tricosane
in acetone
hexachloroethane, hexachlorobenzene, 1,2,4-trichlorobenzene,
o-dichlorobenzene, p-dichlorobenzene, m-dichlorobenzene, hexachloro-
butadiene, 2-chloronaphthalene in acetone
14
-------
CHLORINATED HYDROCARBON
PESTICIDES- WPI
(Method 608)
CHLORINATED HYDROCARBON
PESTICIDES- WPII
(Method 608)
CHLORINATED HYDROCARBON
PESTICIDES - WP III
(Method 608)
CYANIDE. TOTAL
EP METALS
EP PESTICIDES & HERBICIDES
GC/MS ACIDS
(Method 625)
GC/MS BASE NEUTRALS -1
(Method 625)
GC/MS BASE NEUTRALS - II
(Method 625)
GC/MS BASE NEUTRALS-III
(Method 625)
GC/MS PESTICIDES -1
(Method 625)
GC/MS PESTICIDES - II
(Method 625)
HALOETHERS
(Method 611)
ICAP - 19
ICAP-7
NITROAROMA TICS AND
ISOPHORONE (Method 609)
aldrin, dieldrin, DDT, DDE. ODD. andheptachlor in acetone
chlordane in acetone
alpha-BHC, beta-BHC, heptachlor epoxide, endrin, aldehyde, and alpha
and beta endosulfan in acetone
arsenic, barium, cadmium, chromium, lead, mercury, selenium, silver
in acetic acid
lindane, endrin, methoxychlor, 2,4-D, and Silvex in acetone
2-chlorophenol. 2-nitrophenol, phenol, 2,4-dimethylphenol, 2,4-
dichlorophenol, 2,4,6-trichlorophenol, 4-chloro-3-methylphenol,
pentachlorophenol, and 4-nitrophenol in methanol
bis-2-chloroethyl ether, 1,3-dichlorobenzene. 1,2-dichlorobenzene,
nitrosodipropylamine, isophorone, bis-2-chloroethoxy methane. 1,2,4-
trichlorobenzene. hexachlorobutadiene, 2-chloronaphthalene, 2,6-
dinitrotoluene, 2.4-dinitrotoluene, diethylphthalate,
hexachlorobenzene, phenanthrene, dibutyl phthalate, pyrene,
benzo(a)anthracene, dioctyl phthalate, benzo(k)fluoranthene in
methanol
1,4-dichlorobenzene, bis-2-chloroisopropyl ether, hexachloroethane,
nitrobenzene, naphthalene, dimethyl phthalate, acenaphthene,
fluorene, 4-chlorophenyl phenyl ether, 4-bromophenyl phenyl ether.
anthracene, fluoranthene, butyl benzyl phthalate, benzofajpyrene,
benzo(b)fluoranthene, benzo(a,h)anthracene, benzo(g,h,i)perylene in
methanol
4-chlorobenzotrifluoride, m-chlorotoluene, 2,4-dichlorotoluene, 1,3,5-
trichlorobenzene, 1,2,4,5-tetrachlorobenzene, 1.2,3,4- tetrachloro-
benzene. 2,4,6-trichloroaniline, andpentachlorobenzene in acetone
heptachlor, heptachlor epoxide, dieldrin, endrin, ODD, alpha BHC and
gamma BHC
beta-BHC, delta-BHC, aldrin. alpha and beta Endosulfan, 4.4'-DDE, and
4,4'-DDT in acetone
bis(2-chloroisopropyl)ether,bis(2-chloroethoxy)methane, bis(2-chloro-
ethyljether, 4-chlorophenyl phenyl ether, 4-bromophenyl phenyl ether
in acetone
As, Be, Ca. Cd. Co, Cr, Cu, Fe, Mg, Mn. Mo, Ni, Pb. Sb, Se, Ti, Tl, V and
Zn in dilute nitric acid
Ag, Al, B, Ba, K. Na, and Si in dilute nitric acid
isophorone, nitrobenzene, 2,4-dinitrotoluene, and2,6-dinitrotoluene in
acetone
15
-------
PHENOLS (GC)
(Method 604)
PHTHALA TE ESTERS
(Method 606)
POL YCHLORINA TED BIPHENYLS
(Method 608)
POLYNUCLEAR AROMATICS -1
(Method 610)
POLYNUCLEAR AROMATICS - II
(Method 610)
phenol, 2,4-dimethylphenol, 2-chlorophenol, 4-chloro-3-methylphenol,
2,4-dichlorophenol, 2,4,6-trichlorophenol, pentachlorophenol, 2-
nitrophenol. 4-nitrophenol, and 2,4-dinitrophenol in acetone
dimethyl phthalate, diethyl phthalate, di-n-butylphthalate, butyl benzyl
phthalate. diethyl hexyl phthalate and dioctyl phthalate in acetone
separate samples available for Aroclor 1016. 1221, 1232, 1242, 1248,
1254, and 1260 in acetone (laboratory must request specific Aroclor
needed)
acenaphthene, anthracene, benzo(k)fluoranthene, chrysene,
naphthalene, and pyrene in acetone
acenaphythylene, 1,2-benzanthracene, benzo(b)fluoranthene, benzo-
(g.h,i)perylene, benzo(a)pyrene, dibenzo(a,h)anthracene, fluoranthene,
and phenanthrene in acetone
PLEASE NOTE: Distribution of limited quantities of
Standard Reference Material (SRM) 1647 is restricted
to USEPA laboratories, USEPA contractor laboratories,
and state or local government laboratories. Others may
purchase SRM 1647 directly from the National Bureau
of Standards, Office of Standard Reference Materials,
B-311 Chemistry Building, Washington, DC 20234,
(301)921-2045.
CORROSMTY/SODIUM
HERBICIDES
NITRATE/FLUORIDE
CHLORINATED HYDROCARBON
PESTICIDES - WSI
CHLORINATED HYDROCARBON
PESTICIDES - WS II
RESIDUAL FREE CHLORINE
TRACE METALS - WS
TRIHALOMETHANES
TURBIDITY
VOLATILE ORGANIC
CONTAMINANTS -1
(Methods 503, 524, 602 and 624)
VOLATILE ORGANIC
CONTAMINANTS - II
(Methods 503. 524, 602 and 624)
VOLATILE ORGANIC
CONTAMINANTS - III
(Methods 503. 524, 602 and 624)
QC Samples for Drinking Water Analyses
Langlier's Index Value and Sodium in water
2,4-D, 2,4,5-TP (Silvex) in methanol
nitrate-N and fluoride
lindane, endrin, and methoxychlor
toxaphene in acetone
solvent in water
arsenic, barium, cadmium, chromium, lead, mercury, selenium, and
silver
chloroform, bromoform, dichlorobromomethane, and
chlorodibromomethane in methanol
benzene, ethylbenzene, m-xylene, n-propylbenzene, p-chlorotoluene,
1.3,5-trimethylbenzene and p-dichlorobenzene
trichloroethane, p-xylene, o-xylene, t-butylbenzene, p-cymene
and n-dichlorobenzene
toluene, chlorobenzene. isopropylbenzene, sec-butylbenzene,
1,2.4-trimethylbenzene, n-butylbenzene,.and o-dichlorobenzene
16
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VOLATILE ORGANIC
CONTAMINANTS - IV
(Methods 502, 524, 601 and 624)
VOLATILE ORGANIC
CONTAMINANTS - V
(Methods 502. 524, 601 and 624)
VOLATILE ORGANIC
CONTAMINANTS - VI
(Methods 502, 524, 601 and 624)
VOLA TILE ORGANIC
CONTAMINANTS - VII
(Methods 502, 524, 60 J and 624)
1,1-dichloroethylene, cis-1,2-dichloroethylene, 1,1,1-trichloroethane,
1,1-dichloropropene, 1,1,2-trichloroethane, 1,1,2,2-tetrachloro-
ethylene, and bis(2-chloroethyl)ether
bromochloromethane, chloroform, carbon tetrachloride, 1,1,2-trichloro-
ethylene, 1,2-dibromoethane, 1,1,2,2-tetrachloroethane, pentachloro-
ethane, 1,2-dibromo-3-chloropropane and m-dichlorobenzene
dichloromethane, 1,1-dichloroethane, 1,2-dichloroethane,
bromodichlorornethane, 1,3-dichloropropane, 2-chloroethyl ethyl ether,
1,2,3-trichloropropane. chlorobenzene, bromobenzene and o-
dichlorobenzene
trichlorofluoromethane, trans 1,2-dichloroethane, dibromomethane,
1,2-dichloropropane, chlorodibromomethane, 1,1,2,2-
tetrachloroethane, chlorohexane. o-chlorotoluene, and p-
dichlorobenzene
ALGAE FOR IDENTIFICA TION
BACTERIA INDICA TOR STRAINS
CHLOROPHYLL
CHLOROPHYLL
REFERENCE TOXICANTS
SIMULATED PLANKTON
QC Samples for Biology/Microbiology
Sample 1 contains two taxa and Sample 2 contains three taxa of
preserved algae for microscopic identification. Instructions include the
identifications of the algae. (Laboratory must specify sample needed.)
Enterobacter aerogenes, Escherichia coli, Klebsiella peumoniae,
Pseudomonas aeruginosa and Streptococcus faecalis, lyophilized
(laboratory must request specific organisms needed). Also available are
sterile lyophilized blanks for evaluation of aseptic technique.
fluorometric analyses, three levels, in acetone
spectrophotometric analyses, one level, in acetone
sodium lauryl sulfate, one level, aqueous solution, and cadmium
chloride, one level, aqueous solution (laboratory must specify toxicant(s)
needed)
20 mL aqueous suspension of latex spheres for particle counting, and a
permanent, glass slide mount of latex spheres for particle size
distribution determinations
17
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The USEPA Repository for Toxic and Hazardous Materials
EMSL-Cincinnati -maintains the USEPA Repository
for Toxic and Hazardous Materials to provide a
continuing source of calibration materials, standards,
reference compounds, and spiking solutions for all trace
organics of interest to the Agency. The Repository
provides support for Ambient Monitoring, Drinking
Water, NPDES/Priority Pollutants, Hazardous Waste/
Solid Waste, and Toxics Programs.
Compounds are prepared individually as 1.5 ml_
solutions in water-miscible solvents sealed in all-glass
ampuls. A data sheet with each ampul contains general
chemical data, solution specifications, storage and
preservation recommendations, information on purity
and health hazards, and safe handling instructions.
Included with each data sheet is a GC or high perform-
ance liquid chromatograph (HPLC) showing relative
peak areas, retention times of the compound, and
impurities, if any. The chromatograms are obtained
using detector conditions specified in USEPA's
methods.
Three grades of materials will be distributed:
QA Standards (QAS) >99 percent purity
QA Reagents (OAR) 95-98 percent purity
QA Technical Materials (QAT) <95 percent purity
The Repository will move as many compounds as
possible from the QAT and OAR categories into the
QAS category by use of purification techniques.
Exceptions are multicomponent materials such as
PCBs. toxaphene, chiordane, and halowaxes which will
be categorized as OAR or QAT and will not be purified
further. The current list of the Repository materials
distributed is given in the following table:
Concentrations are 5000 fjg of QAS-pure compound per mL of methanol solvent unless otherwise noted.
£001
EO02
EO03
£004
£OOS
£006
£007
£008
EOO9
EO1O
£011
£012
£013
£014
£015
£016
EO17
£018
£019
£020
£021
EO22
£023
E02S
£026
£027
£028
£029
£030
£033
£034
£036
£037
£038
£039
EO4O
£041
£042
£043
£044
EO46
£047
Acenaphthene
Acrolein"
Acrylonitrile (10,000 ng/mL)
Benzene (10.000 iig/mL)
Benzidine
Chlorobenzene flO.OOO fjg/mL)
1.2.4- Trichlorobenzene
Hexachlorobenzene (100O ijg/mL)*
1,2-Dichloroethane
1.1.1'Trichloroethane f1O,OOO (ig/mL) (OAR)
Hexachloroethane
1,1-Dichloroethane (5,500 itg/mL)
1.1.2-Trichloroethane (OAR)
1.1.2.2-Tetrachloroethane flO.OOO fjg/mL) (OAR)
Chloroethane (11,OOO ng/mL)f"
bis(2-Chloroethyl) ether
2-Chloroethyl vinyl ether
2-Chloronaphthalene
2.4.6-Trichlorophenol (OAR)
p-Chloro-m-cresol
Chloroform
2-Chlorophenol
1,2-Dichlorobenzene
1,4-Dichlorobenzene
3,3'-Dichlorobenzidine
1,1-Dichloroethylene (1.00O ftg/mL)
trans-1,2-Dichloroethylene (11.SOO fjg/mLJ
2,4-Dichlorophenol
1,2-Dichloropropane (10.000 pg/mL)
2.4-Dinitrotoluene
2,6-Dinitrotoluene
Ethylbenzene flO.OOO fig/mi)
Fluoranthene
4-Chlorophenyl phenyl ether
4-Bromophenyl phenyl ether
bis(2-Chloroisopropyl) ether (OAR)
bis(2-Chloroethoxy) methane (OAR)
Methylene chloride (10.OOO itg/mL)
Methyl chloride""1
Methyl bromide (9940 iig/mLf"
Dichlorobromomethane
Fluorotrichloromethane
£050 Hexachlorobutadiene (OAR)
£051 Hexachlorocyclopentadiene
£052 Isophorone
£053 Naphthalene
£054 Nitrobenzene
£055 2-Nitrophenol
£056 4-Nitrophe'nol
£057 2.4-Dinitrophenol (OAR)
£058 4,6-Dinitro-o-cresol
£059 N-Nitrosodimethylamine
£060 N-Nitrosodiphenylamine
£061 N-Nitrosodi-n-propylamine
£062 Pentachlorophenol
£063 Phenol
£064 bis(2-Ethyl hexyll phthalate
£065 Butyl benzyl phthalate
£066 Di-n-butylphthalate
£067 Di-n-octylphthalate
£068 Diethyl phthalate
£069 Dimethyl phthalate
EO70 Benzo(a)anthracene (1OOO (jg/mL)
EO71 Benzofa)pyrene(1OOOfig/mL)(QARr
£072 Benzo(b)fluoranthene (2500 fig/mi)*
£073 Benzo(k)fluoranthene (1000 vg/mL)*
£074 ChryseneflOOOfjg/mLr
£075 Acenaphthylene (OAR)
£076 Anthracene (1000 w/mL)*
£077 Benzo(g.h.i)perylene (1000 fig/mi)"
£078 Fluorene (OAR)
£079 Phenanthrene
£081 lndeno(1,2,3-c,d]pyrene (500/ig/mLr
£082 Pyrene (10OO ftg/mL)
£083 Tetrachloroethylene (10.000 fig/mL)
£084 Toluene (10.000 ^g/mL)
£085 Trichloroethylene (1O.OOO (tg/mL)
£088 Dieldrin (1.0OO fig/mi)
£089 Chiordane (QAT)
£091 4.4'-DD£
£092 4.4'-DDD
EO93 alpha-EndosuHan 1 ,OOO fjig/mL"
£094 beta-Endosulfan I.OOOfig/mL"
£095 Endosulfan suHate 1.000 fig/mL (OAR)*11
18
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£096 Endrin (OAR)
E097 Endrin aldehyde (2,500 fig/mi)
£098 Heptachlor
£099 Heptachlor epoxide (2,500 fjg/mLI
£100 alpha-BHC (2500 w/mL)
£101 beta-BHC (2500 ug/mLJ*
£102 gamma-BHC (Lindane)
£103 delta-BHC(10OOiig/mL)
£104 PCB-Aroclor 1242(OAT)
El05 PCB-Aroclor 1254(OAT)
El07 PCB-Aroclor 1232(OAT)
£108 PCB-Aroclor 1248(OAT)
£110 PCB-Aroclor 1016(OAT)
£111 Toxaphene (OAT)
£124 4.4'-DDT(QARI
£125 PCB-Aroclor 1016(1.000(tg/mL)(QATr
El26 PCB-Aroclor 1221 (QATf*
£129 PCB-Aroclor 1260 (500 vg/mL) (QA Tf*
£129 PCB-Aroclor 1260 (1.0OO ag/mLHOA Tf*
El23 PCB-Aroclor 1260(3.000 ijg/mL) (OATf*
El30 PCB-Aroclor 1262(QATr
E131 PCB-Aroclor 1268(2.500 tig/mL)* (OAT)
£132 PCB-Aroclor 1242 (500 (ig/mL) (QA Tf*
£132 PCB-Aroclor 1242(1.000iAg/mL)(OATf*
£132 PCB-Aroclor 1242 (3,000 ag/mLHQA Tf*
£135 PCB-Aroclor 1254 (500 fig/mL) (QA If*
£135 PCB-Aroclor 1254 (1.000 (tg/mLKQA Tf*
£135 PCB-Aroclor 1254 (3.0OO ug/mL)(QA Tf*
£136 Bromochloromethane (10,000 ijg/mL)
E149 2,4-Dichlorotoluene
£150 2-Chlorotoluene
£151 3-Chlorotoluene
£152 4-Chlorotoluene (OAR)
£153 4-Chlorobenzotrifluoride
£156 Pentachloronitrobenzene
£168 alpha, alpha.2,6-Tetrachlorotoluene
£169 Benzyl chloride (OAR)****
£170 2,3-Dichloro-1 -propylene (10,000 ftg/mLI
£171 1,2-Dibromoethane (EDB) (10.0OO fjg/mL)
£173 cis-1,2-Dichloroethylene (10,000 tig/mL) (OAR)
El 75 1.2.3-Trichforobenzene
£176 1,3,5-Trichlorobenzene
£177 1,2,4,5-Tetrachlorobenzene (2500 ug/mL) (QARf
El 79 2,4,5- Trichlorophenol (OAR)
£180 2.4,6-TricMoroaniline
£182 3-Chlorophenol
£183 4-Chlorophenol
£200 Chlorodibromomethane (10,000 fig/mi) (OAR)
£201 ortho-Xylene
£202 meta-Xylene
£203 para-Xytene
£212 Bromoformfl 0,000 ug/mL) (OAR)
£214 1,3-Dichlorobenzene
£218 cis- and trans-1.3-Dichloropropylene (OAR)
£219 MirexflOOOw/rnLr
£220 Aldrin
E222 2.3.5-Trichlorophenol(OAR)
£224 2,4-Dimethylphenol (OAR)
£225 1,2.3,4-Tetrachlorobenzene (25OO iig/mL)
£231 Dibenzo(a,h)anthracene (1OOO fjg/mL)**
£236 n-Decane
£237 n-Undecane
£238 n-Dodecane
£239 n-Tridecane
E240 n-Tetradecane
£241 n-Pentadecane
£242 n-Heptadecane (2500 ijg/mL)
£244 n-Nonadecane (10OO ng/mL)
£250 ortho-Cresol (OAR)
£251 meta-Cresol (QAR>
£252 para-Cresol
£255 Dibutyl ether
£257 Styrene
£258 Epichlorohydrin****
£260 Pentachlorobenzene (2500 fjg/mL}
£261 Dibenzofuran
£262 Diphenyl ether
£263 Diphenylamine
E270 Acrylamide (10.000 fjg/mL)
£271 Pyridine(10.OOO fig/mL)
E282 Diisodecyl phthalate
£284 Acetone
£285 Diethyl ether (4500 (ig/mLJ
£286 1,2-Epoxybutane****
£295 Phenacetin
£298 N-Nitrosopyrrolidine
£299 2-Fluoroacetamide
£300 Pentachloroethane
£305 4-Chloroaniline
£311 Methyl ethyl ketone (10,000 fig/mL)
£322 Methylene bis (o-chloroaniline)
£324 o-Nitroaniline
£325 m-Nitroaniline
£329 Ethylenethiourea
£330 2,4-Dichlorophenoxyacetic acid (2.4-D)**
£334 N-Nitrosodiethylamine
£335 1,1.1,2-Tetrachloroethane (OAR)
£338 Propionitrite
£342 4-Nitroaniline
£349 4-Methyl-2-pentanone
£360 Carbon tetrachloride (10,000 iig/mL)
£363 Carbon disulfide
£368 1,2.3-Trichloropropane
£455 Dinoseb****
£470 PCNHalowax 1099(OAT)
£471 PCNHalowax 1001 (OAT)
£472 PCNHalowax 1000(OAT)
£473 Acetonitrile***
£480 para-Dioxane (10,000 ijg/mL)
£536 Vinyl chloride"*
£541 Benzoic acid****
£542 Aniline
£548 N.N-Dimethylformamide
£552 2,4,5-TP (SilvexJ (QARJ****
£572 Methylparathion (1.000 fjg/mLJ****
£662 3-Nitrophenol
£669 1 -Methyl ethyl benzene (CumeneJ
£688 2-Picoline
£713 Pic/oram (1000 fig/mi)****
£715 Carbofuran
£952 p,p'-Methoxychlor
£954 Aldicarb (1000 fjg/mL)****
£993 1,2-Dibromo-3-chloropropane (QATJ
£995 Aldicarb sulfone (1OOO vg/mL)****
£996 Aldicarb sulfoxide (10OO /jg/mL}****
£1089 Alachlor(1000iig/mL)
£1090 Atrazine (1000 fig/mL)
£1097 Dibromomethane
*ln Acetone
**ln para-Dioxane
***/n 2-Propanol
*Acetonitrile
^Methylene chloride
**//» Isooctane
19
-------
Surrogates and Internal Standard for USEPA/GC/MS Methods 624 and 625
E188 Phenanthrene - H10(150 fjg/mLJ
El 89 Phenol -ds(100 ug/mL)*
E19O 2,4-Dimethytphenol-3,5.6-d3(1OO fjg/mL) (OAR)*
EJ91 Pentachlorophenol - 13C6(10O ng/mL)*
E192 Dimethyl phthalate - ds (150 pg/mL}*
£193 2-Fluorophenol (OAR) (1OO fig/mi)*
El94 2-Fluorobiphenyl(100ijg/mLP
El 95 1-Fluoronaphthalene (1OOfjg/mL)*
El 96 1,4-Dichlorobutane-dg (150 tig/mL)
£197 2-Bromo-1-chloropropane-d6 (150 vg/mL) (QA TJ
£198 Bromochloromethane-d2 (150 itg/mL)
£199 Benzo(g.h,i)perylene-/3C,2 (100 fjg/mL)*
£232 Fluorobenzene (ISO fjg/mL)
£233 4-Bromofluorobenzene (150 fig/mi)
£234 4,4-Dibromooctafluorobiphenyl (100 pg/mL}*
£776 1,2-Dichlorobenzene-d4 (150 pg/mLI
*ln Acetone
*//) para-Dioxane
*»*//! 2-Propanol
*Acetonitrile
*Methylene chloride
' In Isooctane
To obtain QC Samples or Repository Standards,
please fill out the attached request form(s) completely
and legibly and return to EMSL-Cincinnati. Due to initial
small production runs, current Repository orders will
be limited to a single ampul per compound. Allow a
minimum four to five weeks for delivery.
To insure that the QC Samples and Repository
Materials will be used to the best advantage in your
laboratory, we require that the request sheet(s) be
signed by the Laboratory Director or his designee.
Without this approval, QC sample/repository
requests will not be honored.
20
-------
Please Print or Type
Form Approved O.M.B. 2080-0016
4-30-89
Quality Control Sample Request
Name
Company
Laboratory
Street—
City.
.Telephone
. State.
. Zip Code.
Approval of Laboratory Director •:
Check Programs for which samples are requested: —Ambient Monitoring Superfund (CERCLA)
Drinking Water Wastewater Toxics (TSCA) Solid Wastes/Hazardous Wastes (RCRA)
Water Quality/Water Pollution Samples
Demand
EPA/'API Reference Oils
Arabian Light Crude
Prudhoe Bay Crude
— South Louisiana Crude
No. 2 Fuel (high arom.J
No. 6 Fuel (high vise.)
BunkerC
LAS
— Mineral
Nonionic Surfactant Std.
Nutrients
Oil & Grease
Pesticides in Fish
Phenols (4AAP Method)
Suspended So/ids
Ofher
PCBs in Oils
Aro. 1016 in
Aro. 1016 in
Aro. 1016 in
Aro. 1242 in
Aro. 1242 in
Aro. 1242 in
Aro. 1254 in
Aro. 1254 in
Aro. 1254 in
Aro. 1260 in
Aro. 1260 in
Aro. 1260 in
Trace Metals
Trace Metals
Trace Metals
Trace Metals
Other
Capac.
Hydraul.
Trans.
Capac.
Hydraul.
Trans.
Capac.
Hydraul.
Trans.
Capac.
Hydraul.
Trans.
WP-I
WP-II
WP - III
in Fish
Water Supply Samples
WS Corrosivity/Sodium
WS Herbicides
WS Nitrate/Fluoride
WS Chi. Hyd. Pest. I
WS Chi. Hyd. Pest. II
WS Res. Free Chlorine
WS Trace Metals
WS Trihalomethanes
WS Turbidity
WS Vol. Org. Cont. -1
WS Vol. Org. Cont. - II
WS Vol. Org. Cont. - III
WS Vol. Org. Cont. - IV
WS Vol. Org. Cont. • V
WS Vol. Org. Cont. - VI
WS Vol. Org. Cont. - VII
Other
Priority Pollutants/Hazardous Wastes/Toxic Chemicals
.n-Alkanes
. Chlorinated Hydrocarbons
.Chi. Hyd. Pest. WP-I
.Chi. Hyd. Pest. WP-II
.Chi. Hyd. Pest. WP-III
. Cyanide
.EPPest. &Herb.
.EP Metals
.GC/MS Acids
. GC/MS Base Neutrals -1
. GC/MS Base Neutrals - II
. GC/MS Base Neutrals - III
. GC/MS Pesticides -1
. GC/MS Pesticides - II
. Other •
Haloethers
ICAP- 19
ICAP-7
Nitroaro. & Isophorone
PCBs (specific Aroclors)
Aroclor 1016
Aroclor 1221
Aroclor 1232
Aroclor 1242
Aroclor 1248
Aroclor 1254
Aroclor 1260
Phenols (GC)
Phthalate Esters
Polynuclear Aromatics I
Polynuclear Aromatics II
Other
Biological Samples
—Algae for Ident. #1
Algae for Ident. #2
Bacteria Indicator Strains
Enter, aerogenes
E. coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
— Streptococcus faecalis
Sterile Lyophil. Blank
Chlorophyll Fluoro.
Chlorophyll Spectro.
Reference Toxicants
— Sod Lauryl Sulfate
Cadmium Chloride
Simulated Plankton
Other
Other
Date Requested:
EPA-360 (Cin)fRev. 6/83, Pt. 1)
21
. Date Shipped:
-------
Fold Here
Place Stamp
Here
Quality Assurance Branch, Room 525
Environmental Monitoring and Support Laboratory
U.S. Environmental Protection Agency
Cincinnati, Ohio 45268
Fold Here
22
-------
PLEASE COMPLETE THE FORM AND MAIL TO:
QUALITY ASSURANCE BRANCH, Room 525
EMSL-CINCINNATI
U.S. ENVIRONMENTAL PROTECTION AGENCY
CINCINNATI, OH 45268
Form Approved O.M.B. 2080-0016
4-30-89
The USEPA Repository for Toxic and Hazardous Materials
Request for Materials
Please Print or Type
Name
Company
Laboratory
Street
City
.Telephone
. State.
.Zip Code.
Approval of Laboratory Director
Check Programs for which materials are requested: Ambient Monitoring Superfund (CERCLA)
— Drinking Water Wastewater Toxics (TSCA) Solid Wastes/Hazardous Wastes (RCRA)
Concentrations are 5000 ug of QAS-pure compound per mL ofmethanol solvent unless otherwise noted.
.£007 Acenaphthene
.£002 Acrolein**
.£003 Acrylonitrile (10,000 pg/mL)
-E004 Benzene (10,000 tig/mi)
.£005 Benzidine
. E006 Chlorobenzene (10,000 ug/mL)
. £007 1,2,4- Trichlorobenzene
. £008 Hexachlorobenzene (1000 ug/mL)*
.£009 1,2-Dichloroethane
.£010 1,1,1-Trichloroethane
(10,000 ug/mU (OAR)
.£011 Hexachloroethane
.£012 1,1-Dichloroethane (5,500 ug/mL)
. £013 1,1,2-Trichloroethane (OAR)
.£014 1,1,2,2-Tetrachloroethane
(10,000 ug/mL) (OAR)
.£015 Chloroethane (11,000 ug/mL)***
.£016 bis(2-Chloroethyl)ether
.£017 2-Chloroethyl vinyl ether (OAR)
.£018 2-Chloronaphthalene
.£019 2,4.6-Trichlorophenol(OAR)
. £020 p-Chloro-m-cresol
.£021 Chloroform
.£022 2-Chlorophenol
.£023 1,2-Dichlorobenzene
.£025 1,4-Dichlorobenzene
. £026 3,3'-Dichlorobenzidine (OAR)
. £027 1,1 -Dichloroethylene (1,000 fjg/mL)
. £028 trans-1,2-Dichloroethylene
(11,500/jg/mLJ
. £029 2,4-Dichlorophenol
. £030 1,2-Dichloropropane (10,000 ug/mL)
.£033 2,4-Dinitrotoluene
.£034 2,6-Dinitrotofuene
. £036 Ethylbenzene (10,000 /jg/mL)
.£037 Fluoranthene
.£038 4-Chlorophenylphenyl ether
.£039 4-Bromophenylphenyl ether
. £040 bis(2-Chloroisopropyl) ether (OAR)
. £041 bis(2-Chloroethoxy) methane (OAR)
.£042 Methylene chloride (10,000 ug/mL}
.£043 Methyl chloride***
. £044 Methyl bromide (9940 /jg/mL}***
.£046 Dichlorobromomethane
.£047 Fluorotrichloromethane
.£050 Hexachlorobutadiene (OAR)
. £051 Hexachlorocyclopentadiene
.£052 Isophorone
.£053 Naphthalene
.£054 Nitrobenzene
.£055 2-Nitrophenol
.£056 4-Nitrophenol
.£057 2.4-Dinitrophenol (OAR)
.£058 4,6-Dinitro-o-cresol
.£059 N-Nitrosodimethylamine
.£060 N-Nitrosodiphenylamine
. £061 N-Nitrosodi-n-propylamine
.£062 Pentachlorophenol
.£063 Phenol
. £064 bis(2-£thyl hexytj phthalate
.£065 Butyl benzyl phthalate
.£066 Di-n-butyl phthalate
.£067 Di-n-octyl phthalate
.£068 Diethyl phthalate
*ln Acetone **ln para-Dioxane
*ln 2-Propanol
Date Requested:
EPA-360 (Cin)(Rev. 6/83, Pt. 3)
*ln Acetonitriie (compounds continued on reverse)
Date Shipped:
23
-------
-E069 Dimethyl phthalate
-E070 Benzofajanthracene (1000
_ £071 Benzo(a)pyrene (1000 fig/mi) (OAR)*
.£072 Benzo(b)fluoranthene (2500/jg/mL)*
.£073 Benzofkjfluoranthene (1000 fjg/mL)*
. £074 Chrysene (1000 ug/mLJ*
.£075 Acenaphthylene (OAR)
.£076 Anthracene (1000 pg/mL)*
.£077 Benzo(g.h,i)perylene (1000 vg/mL)**
.£078 Fluorene (OAR)
.£079 Phenanthrene
.£081 Indenofl,2,3-c,d)pyrene (500 (jg/mL)*
.£082 PyreneflOOO/jg/mL)
. £083 Tetrachloroethylene (10,000 fjg/mLJ
. £084 Toluene f 10,000 vg/mL)
.£085 Trichloroethylene (10,000 /jg/mL)
.£088 Dieldrin (1,000vg/mL)
.£089 Chlordane (OAT)
.£091 4,4'-DDE
.£092 4.4'-DDD
.£093 alpha-Endosulfan**
.£094 beta-Endosulfan**
.£095 Endosulfan sulfate (OAR)
.£096 Endrin(QAR)
.£097 Endr/n aldehyde (2500 pg/mL)
.£098 Heptachlor
.£099 Heptachlor epoxide (2500 fig/mL)
. £100 alpha-BHC (2500 ug/mL}
.£101 beta-BHC (2500 vg/mLJ*
.£102 gamma-BHC (Lindane)
.£103 delta-BHCflOOOfig/mL)
.£104 PCB-Aroclor 1242 (OAT)
.£105 PCB-Aroclor 1254 (OAT)
.£107 PCB-Aroclor 1232(OAT)
.£108 PCB-Aroclor 1248(OAT)
.£110 PCB-Aroclor 1O16(QAT)
.£111 Toxaphene (OAT)
.£124 4,4'-DDT (OAR)
.£125 PCB-Aroclor 1016(1,000ng/mL)(OATf*
.£126 PCB-Aroclor 1221 (QATf*
.£129 PCB-Aroclor 1260 (500fig/mL)(OAT)"
.£129 PCB-Aroclor 1260 (1.000 ug/mL)(QATf+
.£129 PCB-Aroclor 1260(3,000w/mL)(OAT)"
.£130 PCB-Aroclor 1262(QATf+
. £131 PCB-Aroclor 1268 (2500 fig/mL)* (QA T)
.£132 PCB-Aroclor 1242 (50O fig/mL)(QATr
.£132 PCB-Aroclor 1242(1,000vg/mL)(OAT)"
.£132 PCB-Aroclor 1242 (3.000 pg/mL) (OAT)"
.El35 PCB-Aroclor 1254 (500fjg/mL)(OATf*
.£135 PCB-Aroclor 1254(1,000ug/mL)(OATf*
.£135 PCB-Aroclor 1254(3,OOOw/mL)(QATf*
.£136 Bromochloromethane f 10,000 pg/mL)
.£149 2,4-Dichlorotoluene
.£150 2-Chlorotoluene
.£151 3-Chlorotoluene
.£152 4-Chlorotoluene (OAR)
.£153 4-Chlorobenzotrifluoride
.£156 Pentachloronitrobenzene
.£168 alpha, alpha,2.6-Tetrachlorotoluene
.£169 Benzyl chloride (OAR)****
.£170 2.3-Dichloro-1 -propylene
(10.000 pg/mL)
.£171 1,2-Dibromoethane (EDB) (10,000 pg/mL)
- £173 cis-1,2-Dichloroethylene
(10,000 ijg/mL) (OAR)
.£175 1,2,3-Trichlorobenzene
-El 76 1.3,5- Trichlorobenzene
.£177 1,2,4,5- Tetrachlorobenzene
(2500 vg/mL) (QARf
.£179 2,4,5-Trichlorophenol (OAR)
.£180 2,4,6-Trichforoaniline
.£182 3-Chlorophenol
.£183 4-Chlorophenol
.£200 Chlorodibromomethane
(10,000 w/mL) (OAR)
.£201 ortho-Xylene
.£202 meta-Xylene
.£203 para-Xylene
.£212 Bromoformfl0,000 (jtg/mLJ (OAR)
.£214 1,3-Dichlorobenzene
.E218 cis- and trans-1,3-Dichloropropylene
(OAR)
.£219 Mirex (1000 fjg/mL)*
.£220 Aldrin
.£222 2,3,5- Trichlorophenol (OAR)
.£224 2,4rDimethytphenof (QARJ
.£225 1,2,3,4-Tetrachlorobenzene
(2500 fig/mi)
.£231 Dibenzo(a,h)anthracene (1000 fig/mL)**
.£236 n-Decane
.£237 n-Undecane
.E238 n-Dodecane
.£239 n-Tridecane
.£240 n-Tetradecane
.£241 n-Pentadecane
.£242 n-Heptadecane (2500 jjg/mL)
.£244 n-Nonadecane (1000 fJtg/mLJ
.£250 ortho-Cresol (OAR)
.£251 meta-Cresol(QAR)
.£252 para-Cresol
.£255 Dibutyl ether
.£257 Styrene
.£258 Epichlorohydrin****
.£260 Pentachlorobenzene (2500 ng/mL)
.£261 Dibenzofuran
.£262 Diphenyl ether
*ln Acetone **ln para-Dioxane
*ln 2-Propanol
Date Requested:
EPA-360 (CinJ (Rev. 6/83, Pt. 4)
****ln Acetonitrile (compounds continued on reverse)
Date Shipped:
24
-------
PLEASE COMPLETE THE FORM AND MAIL TO:
QUALITY ASSURANCE BRANCH. Room 525
EMSL-CINCINNATI
U.S. ENVIRONMENTAL PROTECTION AGENCY
CINCINNATI. OH 45268
Form Approved O.M.B. 2080-0016
4-30-89
The USEPA Repository for Toxic and Hazardous Materials
Request for Materials
Please Print or Type
Name
Com pa ny
Laboratory
Street
City
.Telephone.
. State.
. Zip Code-
Approval of Laboratory Director
Check Programs for which materials are requested: Ambient Monitoring Superfund (CERCLA)
Drinking Water Wastewater Toxics (TSCA) Solid Wastes/Hazardous Wastes (RCRA)
Concentrations are 5000 fug of QAS-pure compound per mL ofmethanol solvent unless otherwise noted.
.E263 Diphenylamine
-E27O Aery/amide (10,000 fJig/mL)
.£277 Pyridine (10,000 ug/mL)
-E282 Diisodecyl phthalate
-E284 Acetone
.£255 Diethyl ether
.£286 1,2-Epoxybutane****
.£235 Phenacetin
.£298 N-Nitrosopyrrolidine
.£299 2-Fluoroacetamide
.E30O Pentachloroethane
.£305 4-Chloroaniline
.£311 Methyl ethylketone (10,000 fjg/mL)
.£322 Methylene bis(o-chloroaniline)
.£324 o-Nitroaniline
.£325 m-Nitroaniline
.£329 Ethylenethiourea
.£330 2,4-Dichlorophenoxyacetic acid(2,4-D)*
. £334 N-Nitrosodiethylamine
. £335 1,1,1.2- Tetrachloroethane (OAR)
.£338 Prop/on it rile
.£342 p-Nitroaniline
.£349 4-Methyl-2-pentanone
.£360 Carbon tetrachloride
.£363 Carbon disulfide
£368 1,2,3-Trichloropropane
£455 Dinoseb****
£470 PCNHalowax 1099(OAT)
£477 PCNHalowax 1001 (OAT)
£472 PCNHalowax 1000(OAT)
£473 Acetonitrile***
£450 para-Dioxane (10,000 fjg/mL)
£535 Vinyl chloride ***
£547 Benzole acid****
£542 Aniline
£545 N,N-Dimethylformamide
£552 2,4,5-TP (Silvex) (OAR)****
£652 3-Nitrophenol
£665 7 -Methyl ethyl benzene (Cumene)
£655 2-Picoline
£773 Picloram(1000/jtg/mL)****
£775 Carbofuran
£352 p,p'-Methoxychlor
£354 Aldicarb (1000 ug/mL)****
£333 7,2-Dibromo-3-chloropropane
£335 Aldicarb sulfone
£335 Aldicarb sulfoxide (1000 /jg/mL)
£7053 AlachlorflOOOfjg/mL)
£7030 Atrazine
£7037 Dibromomethane
Surrogates and Internal Standard for USEPA GC/MS Methods 624 and 625
£755 Phenanthrene - dw(150 fjg/mL)
£753 Phenol - ds (100 ug/mL)*
£730 2,4-Dimethylphenol-3,5,6-d3 (100 fig/mL)
(OAR)*
£737 Pentachlorophenol >3C6(100 pg/mL)*
£732 Dimethyl phthalate - d6 (150 tig/mL)*
£733 2-Fluorophenol (QAFtJ (100 ug/mL)*
£734 2-Fluorobiphenyl (100 fjg/mL)*
—£735 7 -Fluoronaphthalene (100 fjg/mL)*
.£196 1,4-Dichlorobutane-da (150 fjg/mLJ
.£197 2-Bromo-1 -chloropropane-d6 (150 ug/mL)
(OAT)
.£198 Bromochloromethane-d2 (150 fjg/mL)
.£199 Benzo(g,h,i)perylene- 13C12 (100 pg/mL)*
.£232 Fluorobenzene (150 pg/mL)
.£233 4-Bromofluorobenzene (150 pg/mL)
.£234 4,4-Dibromooctafluorobiphenyl(100fjg/mL}*
. £776 1,2-Dichlorobenzene-d4 (150 pg/mL)
*ln Acetone **ln para-Dioxane ***ln 2-Propanol
Date Requested:.
****ln A cetonitrile * Methylene chloride
Date Shipped:
In Isooctane
EPA-360 (CinJ (Rev. 6/83. Pt. 5)
25
-------
How to Order USEPA Publications
For Project Summaries (denoted by EPA/600/S
number) of full reports, direct your request to CERI,
USEPA, Cincinnati, Ohio 45268. Be sure to include the
EPA/600/S number and the title for each Summary re-
quested. There is no charge for these publications, but
availability is on a limited basis.
For full reports (denoted by the National Technical
Information Service [NTIS] PB number), direct your
request to NTIS, 5285 Port Royal Road, Springfield,
Virginia 22161. Be sure to include the NTIS PB number,
the report title and a check for the publication(s)
ordered.
Some complete reports (denoted by EPA 600
numbers) are available free of charge on a limited basis
from ORD Publications. Include the EPA/600 number
and the report title with your request. If copies of the
report are no longer available, you will be notified.
These reports may also be obtained at the cost indicated
from NTIS.
Include with your request the NTIS PB number, the
report title, and a check for the publication(s) ordered.
Publications Available
The following publications are now available. The
name of the EMSL-Cincinnati staff person who
served as a project officer or who authored the report
is italicized. Please direct all requests for publications
to the appropriate organization as is indicated below.
Project officers cannot fill publication requests but
welcome technical inquiries.
Inorganic Analyses
Determination of Stable Valence States of Chromium
in Aqueous & Solid Waste Matrices—Experimental
Verification of Chemical Behavior
EPA/600/S4-86/039
NTIS: PB 87 140927/AS ($18.95 per copy)
J. D. Messman, M. E. Churchwell, D. Wong, and J.
Lathouse, and Theodore Martin
QA Reference Books:
Available from:
American Chemical Society Publications
1155-16th Street, N.W.
Washington, DC 20036
(FTS: 737-3337; COML: 202-967-1221)
The Chemical Analysis of Water, 2nd edition (1986)
D. T. E. Hunt & A. L. Wilson Royal Society of Chemistry
(London)
Available from:
AOAC
1111 North 19th Street
Suite 210
Arlington, VA 22209
(COML: 202-522-3032)
Use of Statistics to Develop and Evaluate Analytical
Methods, G. T. Wernimont, W. Spendley, editor (1985),
Association of Official Analytical Chemists (AOAC).
Available from:
National Technical Information Service (NTIS)
U.S. Department of Commerce
5285 Port Royal Road
Springfield, VA 22161
(FTS: 737-4650; COML: 703-487-4650)
Guidance Manual: Test Method Equivalency Petitions
PB 87 178349
Cost $18.00 per paper copy; $6.50 per microfiche
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WHERE TO WRITE
ORD, USEPA, has centralized its publications distribution procedures. When ordering EMSL-Cincinnati
publications cited in the Quality Assurance Newsletter, address all requests to:
ORD Publications
CERI
U.S. Environmental Protection Agency
Cincinnati, OH 45268
Include in your request the appropriate title and the EPA number.
EMSLs can be reached by writing to the following:
U.S. Environmental Protection Agency
Environmental Monitoring Systems Laboratory — Las Vegas
Las Vegas, Nevada 89114
U.S. Environmental Protection Agency
Environmental Monitoring Systems Laboratory — Research
Triangle Park (MD-75)
Research Triangle Park. NC 27711
U.S. Environmental Protection Agency
Environmental Monitoring and Support Laboratory —
Cincinnati
Cincinnati. Ohio 45268
U.S. Environmental Protection Agency (RD-680)
Quality Assurance Management Staff
Office of Acid Deposition,
Environmental Monitoring, and Quality Assurance
Washington. DC 20460
U.S. Environmental Protection Agency
Atmospheric Sciences Research Laboratory—
Research Triangle Park (MD-59)
Research Triangle Park, North Carolina 27711
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Regional QA Coordinators
Wayne Wirtanen
Quality Assurance Coordinator (Water and Air)
Central Regional Laboratory
Environmental Services Division
U.S. Environmental Protection Agency, Region 1
60 Westview Street
Lexington, MA 02173
FTS: 828-6211; COML: 617-861-6700, Ext. 205
Connecticut, Maine, Massachusetts,
New Hampshire, Rhode Island, Vermont
Lloyd Kahn
Quality Assurance Coordinator (Water)
Monitoring Management Branch
Environmental Services Division
U.S. Environmental Protection Agency, Region 2
Edison, NJ 08837
FTS: 340-6709; COML: 201-321-6709
Paul M. Brown
Quality Assurance Coordinator (Air)
Surveillance and Monitoring Branch
Environmental Services Division
U.S. Environmental Protection Agency, Region 2
Edison, NJ 08837
FTS: 340-6766; COML: 201-321-6766
New Jersey, New York,
Puerto Rico, Virgin Islands
Charles Jones, Jr. (3ESOO)
Quality Assurance Coordinator (Water)
. Environmental Services Division
U.S. Environmental Protection Agency, Region 3
841 Chestnut Street, Eighth Floor
Philadelphia, PA 19107
FTS: 597-7210; COML: 215-597-7210
David O'Brien (3ES12)
Quality Assurance Coordinator (Air)
Environmental Services Division
U.S. Environmental Protection Agency, Region 3
841 Chestnut Street, Eighth Floor
Philadelphia, PA 19107
FTS: 597-6445; COML: 215-597-6445
Delaware, Maryland, Pennsylvania, Virginia,
West Virginia, District of Columbia
Wade Knight
Quality Assurance Coordinator (Water and Air)
Laboratory Evaluation and Quality
Assurance Section
Analytical Support Branch
Environmental Services Division
U.S. Environmental Protection Agency, Region 4
College Station Road
Athens, GA 30613
FTS: 250-3390; COML: 404-546-3390
Alabama, Florida,Georgia, Kentucky, Mississippi,
North Carolina, South Carolina, Tennessee
David Payne
Quality Assurance Coordinator (Water)
Quality Assurance Office
Environmental Services Division
U.S. Environmental Protection Agency, Region 5
536 South Clark Street
Chicago, IL 60605
FTS: 353-7712; COML: 312-353-7712
Richard Edmonds
Quality Assurance Coordinator (Air)
Quality Assurance Office
Environmental Services Division
U.S. Environmental Protection Agency, Region 5
536 South Clark Street
Chicago, IL 60605
FTS: 353-9317; COML: 312-353-9317
Illinois, Indiana, Michigan,
Minnesota, Ohio, Wisconsin
Robert Forrest
Quality Assurance Officer/Chief (Water and Air)
Quality Assurance Office (6ES-Q)
Environmental Services Division
U.S. Environmental Protection Agency, Region 6
1445 Ross Avenue
Dallas, TX 75202-2733
FTS: 655-2217; COML: 214-655-2217
Arkansas, Louisiana, New Mexico,
Texas, Oklahoma
Dale Bates
Quality Assurance Officer (Water and Air)
Environmental Services Division
U.S. Environmental Protection Agency, Region 7
25 Funston Road
Kansas City, KS 66115
FTS: 757-3881; COML: 913-236-3881
Iowa, Kansas, Missouri, Nebraska
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Juanita Hillman
Quality Assurance Coordinator (Water)
Environmental Services Division
U.S. Environmental Protection Agency, Region 8
One Denver Place, Suite 1300
999 Eighteenth Street
Denver, CO 80202-2413
FTS: 776-5065; COML: 303-236-5065
William Basbagill
Acting Quality Assurance Coordinator (Air)
Environmental Services Division
U.S. Environmental Protection Agency, Region 8
Denver Federal Center
Post Office Box 25366
Denver, CO 80225
FTS: 776-5097; COML: 303-236-5097
Colorado, Montana, North Dakota,
South Dakota, Utah, Wyoming
Kent Kitchingman
Quality Assurance Officer (Water and Air)
Office of Quality Assurance and
Monitoring Staff (P3-1)
U.S. Environmental Protection Agency, Region 9
215 Fremont Street
San Francisco, CA 94105
FTS: 454-7480; COML: 415-974-0922
Arizona, California, Hawaii, Nevada,
American Samoa, Guam, Trust Territories of
Pacific Islands, Wake island
Barry Towns
Quality Assurance Office/Chief (Water and Air)
Environmental Services Division
U.S. Environmental Protection Agency, Region 10
1200 Sixth Avenue, Mail Stop 337
Seattle, WA 98101
FTS: 399-1675; COML: 206-442-1675
Alaska, Idaho, Oregon, Washington
QA Officer Changes
Region 2
Lloyd Kahn
Quality Assurance Coordinator (Water)
Monitoring Management Branch
Environmental Services Division
U.S. Environmental Protection Agency, Region 2
Edison. NJ 08837
FTS: 340-6709; COML: 201-321-6709
Region 5
Richard Edmonds
Quality Assurance Coordinator (Air)
Quality Assurance Office
Environmental Services Division
U.S. Environmental Protection Agency, Region 5
536 South Clark Street
Chicago, IL 60605
FTS: 353-9317; COML: 312-353-9317
Address and Telephone Number Change
Robert Forrest
Quality Assurance Officer/Chief (Water and Air)
Quality Assurance Office (6ES-Q)
Environmental Services Division
U.S. Environmental Protection Agency, Region 6
1445 Ross Avenue
Dallas, TX 75202-2733
FTS: 655-2217; COML: 214-655-2217
30
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RESPONSE SHEET"
Subject Matter: Water Air Solid Waste
Author:
Comments:
(Use Additional sheets if needed.
Reader's Name
Address
City State Zip Code
Professional Affiliation Phone Number Date
Mail to:
Betty J. Thomas, Publications Assistant
Environmental Monitoring and Support Laboratory-Cincinnati
U.S. Environmental Protection Agency
Cincinnati, OH 45268
(Betty Thomas, FTS: 684-7302; COML: 513-569-7302)
*This response sheet is provided for the reader's use on a voluntary basis. Your thoughts for consideration, suggestions,
and comments are welcome. All inquiries and responses received will be sent to the responsible Agency personnel who
have expertise in the applicable field/subject for review and consideration.
31
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ATTENTION
Mailing List Update
You are currently on the Environmental Monitoring and Support Laboratory's mailing list for the Quality Assurance
(QA) Newsletter.
We are required by the Office of Management and Budget (OMB) to update our mailing list. If you wish to
remain on this list, you must return this page to the address shown below:
Betty J. Thomas
Publications Assistant
EMSL-Cincinnati
26 W. St. Clair Street
Cincinnati, OH 45268
We must receive this page before August 31,1987,
or your name will be removed from this mailing list.
Before mailing this page, detach and return your mailing label, check your name and address, and make corrections
below:
Name — EMSL No.
Street ——
City State Zip Code
If you do not wish to remain on this list, disregard this page.
EMSL 1987 8/87
33
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Mailing List
Quality Assurance Newsletter
EMSL-Cincinnati receives numerous inquiries regarding the computerized mailing list maintained for this Newsletter
and various other mailings. If you are not already on the mailing list, complete the following form and mail to:
Betty J. Thomas
U.S. Environmental Protection Agency
Environmental Monitoring and Support Laboratory-Cincinnati
Cincinnati, OH 45268
If you are already on the mailing list and wish to change your address, delete your name, or change previously specified
Areas of Interest, you must submit your request along with the mailing label found on the back of this Newsletter.
Sending this information greatly speeds our processing of your request.
Cut Here
Name: _
Address:
City/State/Zip Code:
Area of Interest: Circle Applicable Subjects For Each Section
(1) Water:
(a) Chemical Analysis —
Inorganic
(b) Chemical Analysis —
Organic
(c) Aquatic Biology
(d) Microbiology
(e) Viruses
(f) Quality Assurance
(g) Sampling and Automatic
Measurements
(h) Monitoring Systems
(i) Radiochemical Analysis
jj) All Subjects
(2) Air:
(a) Chemical Analysis
(b) Ambient Monitoring
(c) Source Monitoring
(d) Monitoring Systems
(e) Quality Assurance
(f) All Subjects
(3) Solid Waste:
(a) Chemical Analysis
(b) All Subjects
(4) Affiliation: Circle One
(a) US EPA
(b) Other Federal Government
(c) State or Regional Government
(d) Local Government
(e) Industry
(f) Academia
(g) Consultant
(h) Individual
(i) Library
(j) Citizen or Conservation
Group
(k) Foreign
35
-&U. S. GOVKNMENT PRINTING OFIOJ:1987/748-121/67004
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