October 1978
HEALTH EFFECTS RESEARCH LABORATORY
III III II I I I I I I I I
REPORT
i i i i i i i i i i
ABSTRACTS
U.S. ENVIRONMENTAL PROTECTION AGENCY
Health Effects Research Laboratory
Research Triangle Park, North Carolina 27711
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5 ^&7 8 UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
\^M|£^/ HEALTH EFFECTS RESEARCH LABORATORY
<*, ,ort Abstract:; in published quarterly a:; a current, awareness
tool for those who are interested in the activities of the laboratory.
Included are abstracts of Office of Research and Development reports
published during the quarter. If journal or symposia papers by HKKL personnel
have been published during the quarter, their abstract.'; will al.so be included.
Full reports are available (prepaid) from the:
National Technical Information Service
U.S. Department of Commerce
52B5 Port Royal Road
Springfield, Va 22151
(phone: 703/321-8543)
Journal articles or symposia papers are u.sually available from local
libraries. Should you have difficulty in obtaining them locally, you
may write to us for a copy.
If you wish to discontinue receiving these quarterly abstracts, or
nominate additional recipients, please fill in the required information
below, as well as the return address block on the reverse, and return this
sheet to us.
F. Gordon Hueter
Acting Director,
Health Effects Research Laboratory
( ) Please discontinue sending these abstracts to me.
( ) Please send your quarterly Report Abstracts to the addressees listed
below:
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PLACE
STAMP
HERE
(Please include ZIP Code)
U. S. Environmental Protection Agency
Office of Research and Development
Health Effects Research Laboratory
Research Triangle Park, N.C. 27711
Attn: Technical Information Coordinator
Mail Drop 51
(Fold on dotted line and seal before mailing)
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rrCMNICAL REPOHT DATA
icnil Imtnii'liniix tin ll/c if iv.vr /'<•/"'"' ci
I.REPOmNO. 2.
._EPA-f>00/l-78-037c
4. TITLt AND SUI17I I LK
HUMAN SCALP HAIR: AN ENVIRONMENTAL EXPOSURE INDEX FOR
TRACE ELEMENTS. III. Seventeen Trace Elements in
Birmingham, Alabama and Charlotte, North Carolina (1972)
7. AUTHOR(S)
John P. Creason, Thomas A. Hinners and Joseph E.
Bumgarner
8. PERFORMING ORGANIZATION REPORT NO
9. PERFORMING ORGANIZATION NAMT AND ADDRESS
Health Effects Research Laboratory and Environmental
Monitoring and Support Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
RTP.NC
3. RECIPItNT'S ACCESSION- NO.
5. REPORT DATE
__July_J. 978
6. PERFORMING ORGANIZATION CODE
10. PROGRAM ELEMENT NO.
1AA601
11. CONTRACT/GRANT NO.
13. TYPE OF REPORT AND PERIOD COVERED
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Dr. Creason's telephone number is (919) 541-2389
16. ABSTRACT • ~ ~~~
Seventeen trace elements - arsenic (As), barium (Ba), boron, (15), cadmium, (Cd),
chromium (Cr), copper (Cu), Iron (Fe), lead (Pb), lithium (Li), manganese (Mn),
mercury (Hg), nickle (Ni), selenium (Se) , silver (Ag), tin (Sn), vanadium (V), and
Zinc (Zn) - were measured in human scalp hair in two southeastern L'nited States
communities - Birmingham, Alabama and Charlotte, North Carolina. Of the seven for wlii
dustfall trace element measurements were available (lead, nickle, cadmium, copper,
zinc, chromium and manganese) lead showed a significant positive relationship with
male and female children's scalp hair levels, while copper was significantly related
to female childrens' and male adults' scalp hair concentrations, and cadmium levels
were significantly related to scalp hair levels in male adults. Only four out of
sixty tests of significance were significant when housedust was used as an environ-
mental exposure index for fifteen trace elements. This result is about what one
would expect by chance if no differences actually existed. Therefore it appears
that housedust is not an effective index of exposure if in fact seal]) hair levels are
indicators of body burdens of trace elements. Several personal covarintes were
assessed for influences on scalp hair trace element levels for male and female
children and adults. These covnriates are evaluated as potential confounding factors
in future use of hair as an environmental index.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
trace elements
hair
indexes (ratios)
environmental surveys
li.lDENTIFIERS/OPtN ENDCD ILRMS
Charlotte
North Carlina
Birmingham
Alabama
C. COSATI I iclll/(ilollp
06 T, F
8. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
19. St CURITY CLASS (I'his Report)
UNCLASSIFIED
21. NO. OF PAGES
20. SECURITY CLASS (Tltispag
UNCLASSIFIED
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TECHNICAL REPORT DATA
1. REPORT NO.
EPA-600/1-78-043
to. REC.
ACCESSiON-NO.
4. TITLE AND SUBT'.Ti-E
COMPARISON OF METHODS FOR THE ANALYSIS OF PANEL
STUDIES
6. PERFORM;\'G ORGANISATION CODE
7. AUTHORIS)
Victor Hasselblad
8. PERFORMING ORGANIZATION REPORT NO.
Tun: 1978
9. PERFORMING ORGANIZATION NAMt AND ADDRESS
Statistics and Data Management Office
Health Effects Research Laboratory
Research Triangle Park NC 27711
10. PROGRAM ELEMENT NO.
1AA601
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
ILS. Environmental Protection Agency
Research Triangle Park. NC 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP,NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Mr. Hasselblad's telephone number is (919) 541-2337
16. ABSTRACT
Three different methods of analysis of panels were compared using asthma panel
data from a 1970-1971 study done by EPA in Riverhead, New York. The methods were
(1) regression analysis using raw attack rates; (2) regression analysis using the
ratio of observed attacks to expected attacks; and (3) discriminant analysis where
repeated attacks were ignored. The first two methods were found to have serious
serial correlation problems. The third method eliminated this problem, but
reduced the effective sample size considerably.
A more appropriate method was suggested for larger panels over shorter
periods of time. The analyses of the Riverhead data showed that any sulfate
effect on asthmatics was confounded with seasonal trends.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
statistical analysis
epidemiology
asthma
b.lDENTIFIERS.'OPEN ENDED TERMS
•:. COSATi Held'Group
06 F
12 A
3. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
13. SECURITY CLASS >rhii Repvrt]
UNCLASSIFIED
20 SECUfllT^ CLASS ;:'/•';•>•
UNCLASSIFIED
21 NO. cf; °AGES
36
\22. PRICE
EPA Form 2220-1 (9-73)
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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO.
EPA-600/1-78-051
3. RECIPIENT'S ACCESSION-NO.
4. TITLE AND SUBTITLE
Population at Risk to
Data Base "Popatrisk"
Various Air Pollution Exposures
5. REPORT DATE
June 1978
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
Sandor J. Freedman
Joseph D. Wilson
8. PERFORMING ORGANIZATION REPORT NO.
Elsa Lewis-Heise
Albert V. Hardy
9. PERFORMING ORGANIZATION NAME AND ADDRESS
System Sciences, Inc.
P. 0. Box 2345
Chapel Hill, Worth Carolina 27514'
10. PROGRAM ELEMENT NO.
1AA601
11. CONTRACT/GRANT NO.
Contract No. 68-02-2269
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory RTP,NC
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, North Carolina 27711
13..TYP£ OF REPO.RT AND PERIODJCOVEAED.
Final report covering Oct.19/5
Dnr . 1 977 , ..
14. SPONSORING AGENCY CODE
EPA finn/n
15. SUPPLEMENTARY NOTES
Project .Officer is Dr. William Nelson (919-541-2330)
16. ABSTRACT
The work reported herein was undertaken to provide the EPA with a user-
oriented data base containing recent county-based information, for all
counties in the contiguous United States, on population demographics,
population mobility, climatology, emissions, air quality, and age-adjusted
death rates.
The completed data base, called "POPATRISK," contains approximately
27.5 million characters and is in SYSTEM 2000, Version 2.80 format, facili-
tating access with minimal user computer, training. Population demographics
are as of the 1970 Census; population mobility is described spanning the
years 1965 to 1970 for 6 sex-race categories in 7 age groupings for both
"in" and "out" migrants" climatology information contains county summaries
of temperature, precipitation and hours of sunshine; county point and area
source emission estimates are provided for 5 criteria pollutants--TSP, SO^,
N02, CO, and Ozone--based on the NEDS-USER file; air quality information is
based on 1974 data contained in SAROAD; age-adjusted death rates were computed
for the combined years 1969, 1970, and 1971 for 4 sex-race categories in
50 groupings of ICDA categories (8th revision).
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS C. COSATI Field/Group
data file
air quality
demography
population
mortality
Population at risk
05A
09B
06F
18. DISTRIBUTION STATEMENT
Release to Public
19. SbCUHITY CLASS (Tltis Report)
Unclassified
21. NO. OF PAGES
140
20. SECURITY CLASS (Thispage)
Unclassified
22. PRICE
EPA Form 2220-1 (9-73)
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DAI A
(/'/.WAV mitl lutJfiti'tiiiiis on <-V ;NO.
Q. REPORT DATE
August 1978
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
8. PERFORMING ORGANIZATION REPORT NO.
Margaret A. Kelsall and Ruth E. Hunter
9. PERFORMING ORGANIZATION NAME AND ADDRESS 10. PROGRAM ELEMENT NO.
Ohio Mental Health and Mental Retardation Research Center 1AA601
1708 Aiken Avenue
Columbus, Ohio
11. CONTRACT/GRANT NO.
R-804632
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP, NC. .
14. SPONSORING AGENCY CODE
EPA 600/11
IS. SUPPLEMENTARY NOTES
Project Officer is Warren Galke (919-541-2862)
16. ABSTRACT -
An epidemiologic study of the distribution of lead absorption in the Cleveland
Metropolitan Area was carried out by analyzing the lead content of shed or extracted
deciduous teeth of 11,241 children. Mean amounts of lead in teeth of children
living in some suburbs were as high as those for children living within the city
of Cleveland. Levels of lead in teeth declined with increasing age at tooth loss.
Efforts were made to relate tooth lead levels to various environmental sources of
lead. Also teacher evaluations of children's school performance and behavior were
assessed in regard to tooth lead level and environmental exposure.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
lead
teeth
children
epidemiology
b.lDENTIFIERS/OPEN ENDED TERMS
Cleveland
c. COSATI Held/Group
06 F, T
8. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
19. SECURITY CLASS (ThisReport)
UNCLASSIFIED
21. NO. OF PAGES
169
20. SECURITY CLASS (Tillspage)
UNCLASSIFIED .
22. PRICE
EPA Form 2220-1 (9-73)
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r?«:.HM!CAL REPORT DATA
1. REPORT MO. i2.
EPA-600/1-78-054 |
4. TITLE AND SUBTITLE "
Chromosomal Aberrations in Peripheral Lymphocytes of
Students Exposed to Air Pollutants
3. RECIP'ENT'S ACCESSIOf*NO.
... RET-ORT DATE
August 1978
6. PERFORMING ORGANIZATION CODE
7. AUTHORIS)
Charles D. Scott and John A. Burkart
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAMC AND ADDRESS
Utah Biomedical Test Laboratory
University of Utah Research Institute
520 Wakara Way
Salt Lake City, Utah 84108
10. PROGRAM ELEMENT NO.
1AA601
11. CONTRACT/GRANT NO.
68-02-1730
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP,NC
14. SPONSORING AGENCY CODE
EPA-600/11
15. SUPPLEMENTARY NOTES
Project Officer is Dr. Dorothy C. Calafiore (919-541-2676)
16. ABSTRACT
This research program was initiated with the overall objective of determining
whether or not photochemical air pollutants have the potential to cause chromosome
breakage in environmentally exposed individuals; if so, could chromosomal changes be
used as a biological indicator of exposure to certain environmental conditions in the
Los Angeles, CA basin.
256 incoming Freshmen students at the University of So. California were selected,
matched, and grouped by home address into in-basin males and females, and out-of-basin
males and females. Blood samples were collected from them at the following sampling
time: October 1974, February, May and October 1975, and May 1976. All slides were
analyzed in a double blind fashion, with 100 cells per student per sampling time being
scored. All 100 cells were analyzed for chromosome and chromatid aberrations; however,
only 25 cells of this 100 were counted for aneuploidy. Overall, in-basin males had
significantly more abnormal cells, breaks, and gaps than out-of-basin males. Females
showed the same trends but only for abnormal cells were the results borderline statis-
tically significant. Differences between the two groups of students were more pronoun-
ced at both October evaluations than at the February and May evaluations. Chromosome
abnormalities in general showed increases from October 1974 through May 1975 and then
decreased by October 1975. These changes followed similar trends in levels of carbon
monoxide, nitrogen oxides, and ozone.
17.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
carbon monoxide
nitrogen oxides
ozone
chromosome abnormalities
air pollution
b.lDENTIFIERS/OPEM ENDED TERMS
students
Los Angeles
California
COSATl Held/Group
06, F
13. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
\-3 -FCURiTY CLASS 'I'lth Keptirt,
\ UNCLASSIFIED
f~20. SECUfliTY CLASS .•'',: : .:•.•.-;
i UNCLASSIFIED
21. NO. O
172.
PAGES
22.
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TSTHMICAL Pi
•/V -.:»,• ;<•;..' /•:.•.••I.V/II'/IA ,:;• «/..
; i' DAY A
1. REPORT NO.
EPA-600/1-78-055
4. TITLE AND SUBTITLE
EPIDEMIOLOGIC STUDY OF THE EFFECTS OF AUTO!-
TRAFFIC ON BLOOD LEAD LEVELS
3. RtClrl.NT'S ACCLSS.ON MO.
!.. Ill I'OitV DM! I'
jE i .—August... 19.7.8___.
5. PtRrCHMiNG ORGANIZATION
7. AUTHOK(S)
D. Johnson, R. Prevost, J. Tillery, K. Kimball,
J. Hosenfeld
9. PtRPORMING OHGANIZA11GN NAML- AM.' ADUSl SS
Southwest Research Institute
3600 Yoakum Blvd.
Houston, Texas 77006
i
3. CtHrOriMING ORGANIZATION REHOI11 NO.
. HlUK,,iAM LLLMLn NO.
1AA601
;i1. CONTIiACT/GRANT NO.
! 68-02-2227
12. SPONSORING AGENCY NA.V.F AND ALOfJF.SS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, NC 27711
RTP, NC
113. TYPE OF RsTORT AND I'ERIOD COVCHED
!;l».\sijoN£;oR!N~cf At;tfviCY coot
EPA 600/11
15. SUPPLEMENTARY NOTcS
Project Officer is Warren Galke (919-541-2862)
16. ABSTRACT
This study investigated the absorption of lead by persons of. different age-sex
groups exposed to automobile emissions of lead at traffic densities from less than
1,000 cars per day to 25,000 cars per day. The relationships between traffic
density and lead in various environmental samples were also examined. A house-to-
house survey based on a strict set of selection criteria was used to recruit study
participants. At each house a series of environmental measurements were taken:
traffic volume, tap water, paint-interior and exterior, housedust and window sill
wipes. Two blood samples were taken a week apart. In the range of traffic
exposures studied no relationship with blood lead levels was observed (maximum mean
air lead < 2.0 yg/m^). A positive relationship between smoking and blood lead
levels was found for both males and females. This relationship was statistically
significant for females but not for males.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
lead
blood analysis
toxicity
automobiles
vehicular traffic
epidemiology
environmental surveys
b. IDENTIFIERS/OPEN ENDhD TERMS
Dallas
Texas
COSATI l-'iciil/Cioup
06 F, T
8. DISTRIBUTION STATEMENT
19. SECURITY CLASS (This Rrporti
___ UNCLASSIFIED..-.
2O. SECURITY CLASS I This
21. NO. OF PAGES
22. PRICE
UNCLASSIFIED
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liCMNICAI. KF.I't IT DATA
jl'i- ::• i,;t.l li;\lnti'll<>ll* I'll I/It'll MI /••(»/(• ruiii/'.'i I'D.T
1. REPORT NO.
JiPA-bOO/ J.778-Q.'J6
4. TITLE AND SUBTITLE
TERATOLOGY OF GUTHION
7. AUTHOR(S)
Robert D. Short, Jan L/ Minor, Timothy M. Unger, and
Cheng-Chun Lee
9. PERFORMING ORGANIZATION NAMt AND ADDRESS
Midwest Research Institute
425 Volker Blvd.
Kansas City, MO 64110
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
U.S. Environmental Protection Agency
Office of Research and Development
Research Triangle Park, N.C. 27711
RTP.NC
3. Ml Cll"l N I 'S ACCbGSIOI^NO.
nr"om OATH
August. . llJ7i>.
6. PERFORMING ORGANIZATION CODE
8. PERFORMING ORGANIZATION REPORT NO.
10. PROGRAM ELEMENT NO.
1EA613
11. CONTRACT.-GRANT NO.
68-02-2746
13. TYPE OF REPORT AND PERIOD COVERED
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Project Officer is Dr. Ronald L. Baron (919-541-2655)
16. ABSTRACT
The purpose of this study was to assess the effects of Guthion, a pesticide
with auticliolinesLerasc activity, on development in rats and mice. A preliminary
toxicity study with Guthion indicated that a 35 LD^ dose for virgin rats and a 10 day
LD5Q dose for virgin mice was between 4 and 8 mg/kg/day for both species. On the
basis of this data, doses of 0, 1.25, 2.5, and 5.0 mg/kg/day were selected for the
developmental study, which consisted of two phases. During the first phase, pregnant
rats and mice were treated for 10 days starting on gestational day f>. The high dose
affected maternal welfare only in rats. Guthion did not significantly increase in a
dose-related manner any of the specific anomalies observed in either ruts or mice.
During the second phase, pregnant rats were treated from gestational day 6 to post-
partum day 21. Diims in the high dose group were more sensitive to Guthion later in
gestation with the result that deaths and signs of anticholinesterase toxicity
increased during this time. Guthion also adversely affected maternal welfare in this
group. As a result of Guthion toxicity, only one litter survived until weaning. The
inability to dissociate toxicity in adult and developing animals suggests that Guthion
has little primary effect on the development of rats or mice.
DESCRIPTORS
pesticides
cholinesterase inhibitors
KEY WORDS AND DOCUMENT ANALYSIS
I>.IIM:NTIHLHS/OI'LN I NIJI D II HMS 0. COSATI I k-UI/Clrotip
Guthion
teratology
06
3. DISTRIMUTION STATEMENT
RELEASE TO PUBLIC
19 RFCURITY CLASS i I'lu* Kef"'!'
_.._
20. SI.CUmTY CLASS i'j'liii
UNCLASSIFIED
21. NO. OI- PAGES
__25
22. PRICE
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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO.,
EPA-600/1-78-059
3. RECIPIENT'S ACCESSION-NO.
4.TITLC. AND SUBTITLE
Follow-up of Patients Receiving Diagnostic Doses of
131 Iodine During Childhood
5. REPORT DATE
September 1978
5. PERFORMING ORGANIZATION CODE
7. AUTHORISI
Benjamin S. H. Harris, III, Martha L. Smith,
Mildred I. Holt
9. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Research Triangle Institute
P. 0. Box 12194
: Research Triangle Park, N. C. 27709
10. PROGRAM ELEMENT NO.
1FA628
117CONTRACT/GHANT NO.
Contract No. 68-02-1213
12. SPONSORING AGENCY NAME AND ADDRESS -
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, North Carolina
13. TYPE OF REPORT AND PERIOD COVERED
Final report covering 6/73-5/7
14. SPONSORING AGENCY CODE
27711
15. SUPPLEMENTARY NOTES
Project.Officer is Dr. William Nelson (919-541-2330)
16. ABSTRACT
This report documents the data collection methodology and procedures of a
follow-up survey conducted of persons under 16 years old who received diagnostic
Iodine 131 for evaluation of thyroid function at nine clinical centers prior to
December 31, 1960. The intent of this data collection effort is to estimate
the dose response curve for the development of thyroid neoplasms in young:adults
who received low diagnostic doses of Iodine 131 as children.
Of 2,287 potential study subjects identified, some medical record was
reviewed and abstracted for 1,999 or some 87 percent. . With study activities
incomplete, of 186 private physicians and other referral sources Contacted for
supplemental data, only three or less than two percent declined to participate;
of 1,362 patients who were determined eligible and entered the survey phase,
some final resolution (completed questionnaire, death certificate, or refusal)
was obtained for 1,065 or some 78 percent.
The statistical analysis of these data will be performed under a related
project sponsored by the U. S. Food and Drug Administration.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS
COSATI Field/Group
thyroid
iodine
diagnostic dose
fof low-up
Follow-up study
3. DISTRIBUTION STATEMENT
Release to Public
19. SECURITY CLASS (This Report)
unclassified
21. NO. Or PAGES
68
2O. SECURITY CLASS (Thispage)
unclassified
22. PRICE
EPA Form 2220-1 (9-73)
10
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TECHNICAL REPORT DATA
(Please read Inuructions on the re'.-ene before completing)
I. REPORT NO.
EPA-600/1-78-060
2.
3. RECIPIENT'S ACCESSION-NO.
4. TITLE AND SUBTITLE
5. REPORT DATE
Toxaphene Composition and Toxicology
September 1978
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
John E. Casida and Mahmoud Abbas Saleh
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Pesticide Chemistry and Toxicology Laboratory
Department of Entomological Sciences
University of California
Berkeley, CA 94720
10. PROGRAM ELEMENT NO.
1EA615
11. CONTRACT/GRANT NO.
R-803913
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP,NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Project Officer is Dr. Ronald L. Baron (919-541-2655)
16. ABSTRACT
The composition and metabolism of Toxaphene have been examined to aid in understanding
the conditions under which this insecticide can be most effectively and safely used. Each
of 8 Toxaphene samples manufactured by Hercules Chemical Co. from 1949 to 1975 shows the same
29 major peaks and in almost identical ratios. About 85% of the total peak area is accounted
for by these 29 peaks which individually vary from 1 to B\ of the total. The 8 Toxaphene
samples were easily differentiated from 12 samples of chlorinated terpenes from other manufac-
turers in the United States and abroad. There is surprisingly little variation in the acute
toxicity of any sample.
Five major Toxaphene components (2,2,5-endo,6-exo,8,9,10-heptachtotobornane (T) and its
3—cxo-chloro-, 8-chloro—, 9-chloro- and 10-chloro—derivatives) collectively account for up to
23% of the technical grade Toxaphene and up to 34* of those of chlorinated 2-exo,10-dichloro-
bornane. Chlorination of 2-exo, 10-dichlorobornane providts a convenient s;ource of I and other
chlorinated bornanes. The toxicity to mice, houseflies and goldfish of the octachlorobornanes
formed by introducing chlorine substituents into I, relative to I itself, generally decreases
in the order: 9-chloro > 8-chloro > no added chlorine (i.e. I) > 3-exo-chloro, 5-exo-chloro or
10-chloro.
Fat from chickens and mammals treated orally with Toxaphene contains products similar in
GLC characteristics to Toxaphene itself whereas liver and feces contain Toxaphene-derived
products of greatly altered GLC properties. Toxaphene preparations and related chlorinated
terpenes are mutagens in the histidine-requiring Saljionella typhimurium assay. The most
potent mutagenic components, which are not identified, reside in the polar fractions on
crystallization or solumn chromatography.
17.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS
c. COSATI Field/Group
insecticides
metabolism
composition(property)
toxicity
Toxaohene
07 C
06 A, T
13. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
13. SECURITY CLASS ( FMs Report)
UNCLASSIFIED
21. NO. OF PAGES
65
120. SECURITY CLASS (Tills page/
22. PRICE
UNCLASSIFIED
11
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MS-76-035
TECHNICAL REPORT DATA
(Please read Instructions on the .-crerse before completing)
1. REPORT NO.
2.
3. RECIPIENT'S ACCESSION NO.
4-
4. TITLE AND SUBTITLE
POTENTIAL EXPOSURE FROM SMOKING PARATION-CONTAMINATED
CIGARETTES
!5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
S.W. Comer, A.L. Robbins and D.C. Staiff
8. PERFORMING ORGANIZATION REPORT NO.
I
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Field Studies Section, Environmental Toxicology Div.
Health Effects Research Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
10. PROGRAM ELEMENT NO.
1EA6L5
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
Health .Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP,NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Arch. Environ. Contam. Toxicol. 6:103-110, 1977
16. ABSTRACT
Pesticide workers usually contaminate their cigarettes with less than 100 yg per
cigarette by handling them, but in some instances such contamination has been found
to be over 200 yg. To obtain values for potential exposure resulting from smoking
contaminated filter tip and non-fileter cigarettes, from 10 to 200 yg of parathion
was applied to'the cigarette surface are found to be most often contaminated by hands.
This was followed in two separate experiments by simulated smoking using a special
apparatus to determine (1) passage of toxic material through cigarettes in mainstream
smoke, (2) amount found in sidestream smoke, (3) amount trapped in the filter or
equivalent butt end, and (4) amount recovered in ashes. The amount of parathion
passing through cigarettes as a potential for inhalation exposure ranged up to 28%
of dosing levels. No paraoxon or S-ethyl parathion was detected.- Although there
was an indication that slightly more pesticide was recovered from filters than from
equivalent butt ends, the amount recovered in mainstream smoke of filter type
cigarettes was not significantly lower than for nonfilter cigarettes. Location of
contamination on a cigarette had minimal effect on the amount found in mainstream
smoke.
17.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS
c. COSATI F-'ield/Group
contamination
toxicity
chemical analysis
Parathion
cigarette smoking
06, T
07, C
18. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
19. SECURITY CLASS (This Report)
UNCLASSIFIED
21. NO. OF PAGES
20. SECURITY CLASS 'This page/
UNCLASSIFIED
22. PRICU
£PA Form 2220—1 \Rev. 4-77) PREVIOUS EDITION
12
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MS-76-069
TECHNICAL REPORT DATA
(Please read Instructions <;n :tie re:-er,c hejorc comrU t
1. REPORT NO.
! 2.
|3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
EFFECT OF OZONE ON LEUKOCYTE FUNCTION IN EXPOSED
HUMAN SUBJECTS
5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
AUTHOR(S)
M.L. Peterson, S. Harder, Nicholas Rummo, and D. House
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Clinical Studies Division
Health Effects Research Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
j 10. PROGRAM ELEMENT NO.
i 1AA601
111. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
I 13. TYPE CF REPORT AND PERIOD COVERED
RTP.NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Environ. Res. 15:485-493, 1978
16. ABSTRACT
Evidence from animal studies indicates that ozone (03), the major component of
environmental photochemical smog, depresses various intracellular hydrolytic enzymes
and increases susceptibility to microbial infections. It would appear possible that
03 induced alterations in normal leukocyte functions might underlie some of these
reported alterations in human response to infectious agents. A study was designed to
assess the effect of 03 on peripheral blood leukocytes from 21 healthy young human
males exposed to 784 yg/m3 of ozone for 4 hr. The capability of polymorphonuclear
neutrophils to phagocytize and kill microorganisms of respirable size was evaluated
and phagocytic and bactericidal rates were determined. A significant decrease in
intracellular killing was seen at 72 hr. postexposure (p < 0.001). A decrease in
phagocytic ability was also noted at 72 hr (p < 0.05). These findings indicate that
03 in low concentrations has a transient effect on leukocyte functions in humans. Such
an effect may be mediated by alterations in cell membrane, opsonization functions,
and/or interference with intracellular enzyme synthesis.
17.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lOENTIFIERS/OPEN ENDED TERMS
CCSAT: Hold/Group
ozone
immunity
lymphocytes
environmental tests
06 ?
18. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
EPA Form 2220-1 iRev. 4-77) P^EVIOOS E;I-'V
i 19. SECURITY CLASS (Tins Hiportl
21. NO. Of PAGES
'.20 SECURITY C^ASS i Hits paqti
\ UNCLASSIFIED
13
22. PRICE
-------
MS-77-014
TECHNICAL REPORT DATA
(Please read Instructions' on the reverse before completing)
1. REPORT NO.
2.
3. RECIPIENT'S ACCESSION>NO.
4. TITLE AND SUBTITLE
EXPOSURE OF FERTILIZER MIXING PLANT WORKERS TO
DISULFOTON
5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
8. PERFORMING ORGANIZATION REPORT NO.
H.R. Wolfe, D. C. Staiff, J.F. Armstrong, and J.E. Davis
9. PERFORMING ORGANIZATION NAME AND ADDRESS
ies Section,, ETD
ects Research Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
Field Studii
Health Effec
10. PROGRAM ELEMENT NO.
1EA615
11. CONTRACT/GRANT NO.
12^SPQNSPR1NG.AGENCY NAME AND ADDRESS
Health Errects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
RTP.NC
13. TYPE OF REPORT AND PERIOD COVERED
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Bull. Environ. Contain. Toxicol. 20:79-86, 1978
16. ABSTRACT
The potential hazard-to workers exposed to the organophosphorus pesticide,
disulfoton/ in fertilizer-pesticide mixing plants was studied. Potential dermal and
respiratory exposure,- as well as levels of pesticide in air near certain work
stations, ^as determined. Exposure was found to be much higher during dry mixing
of granular pesticide with pelletized fertilizer than during the wet mixing process
involving injection of liquid concentrate pesticide into the system to evenly
impregnate the fertilizer. Calculations based on the highest exposure values obtained
indicate that a worker may be subjected to as much as 4.3% of a toxic dose per hour
of exposure during dry mix operations, but only 0.4% of a toxic dose per hour of
exposure during wet mix operations. Levels of disulfoton in most of the air samples
taken in the plant during dry mix operations exceeded the 0.1 mg/M^ threshold limit
value for that compound; however, none of the samplings during wet mix operations
exceeded that level. Although none of the values obtained for blood cholinesterase
were below the low normal values for the general population, some of the workers
involved in dry mix operations did show a gradual decrease in their red cell values
with time of exposure. It is suggested that workers exposed longer than three months
be given special attention as far as medical surveillance is concerned.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.IDENTIFIERS/OPEN ENDED TERMS
c. COSAT! Field/Group
fertilizers
pesticides
environments
safetv
disulfoton 06 F, T
organophosphorus compounc s
8. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
19. SECURITY CLASS tTtiiSReport)
UNCLASSIFIED
21. NO. OF PAGES
120. SECURITY CLASS iTlliS pa^e)
UNCLASSIFIED
22. PRICE
aPA Form 2220-1 (9-73)
14
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MS-77-032
TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing/
1. REPORT NO.
2.
3. RECIPIENT'S ACCESSION-NO.
4. TITLE AND SUBTITLE
Analytical Methodology for the Determination of
Kepone(R) Residues in Fish, Shellfish, and Hi-Vol Air
Filters
5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
7. AUTHORIS)
David W. Hodgson, Edward J. Kantor, and J. Bruce Mann
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Environmental Toxicology Division
Health Effects Research Laboratory
Office of Research and Development
Triangle Park, N.fL 27711
10. PROGRAM ELEMENT NO.
EA615
.1EAJ
. CONT
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory .
Office of Research and Development
U.S. Environmental Protection Agency
Pooaarrh Tr-ianplp Park. NH 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP,NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Arch. Environ,. Contain. Toxicol. 7:99-112, 1978
16. ABSTRACT
The recent discovery of the pollution of the environment with Kepone has resulted
in a tremendous interest in the development of residue methodology for the compound.
Current multiresidue methods for the determination of the common organochlorinated
pesticides do not yield good quantitative analytical results for Kepone. The
extracting solvents are usually of insufficient polarity to extract Kepone from the
various media. This article describes some of the recently developed methodology
for Kepone in air filters, finfish, finfish livers and entrails, shellfish, and
archival oyster samples.
The finfish and archival oyster samples were Soxhlet extracted using diethyl
ether/petroleum and ether (1:1 v/v) as the extracting solvent. The finfish livers
and entrails were macerated in a Duall tissue grinder containing acetonitrile
followed by partitioning of the Kepone into benzene. Shellfish samples were analyzed
after extracting the sample with acetonitrile and partitioning the Kepone into benzene
The Hi-Vol air samples were extracted with methanol/benzene (1:1 v/v) The coextrac-
ting contaminants were removed by micro-Florisil column chromatography and/or acid
.digestion. The procedural recovery of Kepone from fortified samples averaged 82%.
17.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS C. COSATI I ieid/GrouD
pesticides
chemical analysis
biological extracts
air
Kepone
06, F
07, C
13. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
; 19. SECURITY CLASS , T/II.V .\> ^TII
I
FT ED
21. NO. OF FAGE3
- 20. -SECORITV CLASS • 7V:ii ,"a-,v
I UNCLASSIFIED
EPA Form 2220-1 (9-73)
15
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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing;
1. REPORT NO.
iJ. RECIPIENT'S ACCESSION NO.
I
4. TITLE AND SUBTITLE
INFLUENCE OF CADMIUM, NICKEL, AND CHROMIUM ON PRIMARY
IMMUNITY IN MICE
5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
. AUTHOR(S)
J.A. Graham, F.J. Miller, M.J. Daniels, E.A. Payne,
and D.E. Gardner
8. PERFORMING ORGANIZATION REPORT NC
. PERFORMING ORGANIZATION NAME AND ADDRESS
Clinical Studies Division
Health Effects Research Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
10. PROGRAM ELEMENT NO.
1AA601
11. CONTRACT/GRANT NO.
12, SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research?,Triangle Park, N.C. ,27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP, NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Environ. Res. 16:77-87, 1978
16. ABSTRACT
; The effects of metals on the primary humoral immune system of mice were investiga-
ted using a hemolytic plaque technique to determine the number of specific antobody-
producing spleen cells. Inhalation of NiCl2 for.2 hr resulted in a significant negativ
linear dose response, the lowest effective concentration tested being 250 yg of
Ni/m3. Following a 2 hr aerosol exposure to NiCl2, the lung cleared Ni on a first-orde
kinetics basis. A significant reduction in the number of plaques per 10*> cells was
also observed with exposure to 190ygof Cd/m-*. Analyses of the data from intra-
muscularly exposed mice indicated that concentrations greater than or equal to 3.90
yg of Ni/g body weight (as NiS04) and greater than or equal to 9.25 yg of Ni/g body
weight (as NiCl£) resulted in significant immunosuppression. Intramuscular treatments
with NiO, CdCl2, and CrCl3 had no effect at the concentrations tested.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS
c. COSATI Field/Group
cadmium
nickel
chromium
immunity
06 F
18. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
EPA Form 2220-3 (R«v. 4-775 PREVIOUS EDITION a OBSO..ETE
19. SECURITY CLASS ,This Report>
UNCLASSIFIED
21. NO. OF PAGES
20. SECURITY CLASS (Thupagei
UNCLASSIFIED
22. PRICE
16
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SS-77-049
TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing/
1. REPORT NO.
2.
3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
MASS SPECTROMETRIC ANALYSIS AND CHARACTERIZATION OF
KEPONE IN ENVIRONMENTAL AND HUMAN SAMPLES
5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
R.L. Harless, D.E. Harris, G.W. Sovocool, R.D. Zehr,
N.K. Wilson, and E.O. Oswald
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Environmental ToxicologyT Division
Health Effects Researcn Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
10. PROGRAM ELEMENT NO.
1EA615
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
RTP,NC
13. TYPE OF REPORT AND PERIOD COVERED
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Biomed. Mass Spectrometry 5(3):232-237, 1978
16. ABSTRACT
A specific portion of our environment has been contaminated with Kepone, or
chlordecone. Additionally, some specific human exposures to high concentrations
of Kepone have been confirmed. Gas chromatography mass spectrometry involving
chemical ionization and high resolution mass spectrometry were used to detect,
identify and confirm the presence of Kepone, Kepone photoproducts and a reduction
product of Kepone in environmental and human samples. Field desorption, field
ionization and electron impact mass spectrometric methods, as well as infrared
and nuclear magnetic resonance techniques were used to characterize and identify
Kepone hydrate and hemiketalin benzene and methanol solutions, respectively.
17.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS C. CCSATI Field/Group
pesticides
chemical analysis
biological extracts
Kepone
06, F
07, C
13. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
119. SECURITY CLASS i Tins Report i
TTNTPT
21. NO. OF PAGES
|20. SECURITY CLASS This pare I
i UNCLASSIFIED
122. PRICE
EPA Form 2220-1 (9-73)
17
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MS-77-053
TECHNICAL REPORT DATA
(Please read Instructions on the rtferse before wmpienng)
1. REPORT NO. 2.
4. TITLE AND SUBTITLE
EXPOSURE OF PESTICIDE FORMULATING PLANT WORKERS TO
PARATHION
7. AUTHOR(S)
H.R. Wolfe, D.C. Staiff and J.F. Armstrong
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Field Studies Section, ETD
Health Effects Research Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
12. SPONSORING AGENCY NAME AND ADDRESS
Health ., Effects, Research Laboratory RTP,NC
Office of Research and Development
Ui S . Environmental Protection Agency
Research Triangle Park, N.C. 27711
3. RECIPIENT'S ACCESSION NO.
5. REPORT DATE
6. PERFORMING ORGANIZATION CODE
1
8. PERFORMING ORGANIZATION Rcr>ORT NO
10. PROGRAM ELEMENT NC.
1EA61 5
11. CONTRACT/GRANT NO.
13. TYPE OF REPORT AND PERIOD COVERED
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: Bull. Environ. Contain. Toxieol. 20:340-343,. 1978
16. ABSTRACT ~~~ " \
- The potential hazard to workers exposed to the organophosphorus pesticide,
parathion, in formulating plants was studied. Potential dermal and respiratory
exposure.near .certain work stations was determined. Calculations were based on the
use of minimum protection (no respirator, shirt with short sleeves and open collar, no
hat, no gloves, and with the assumption that the clothing worn gave complete protection
of body areas covered). This was to arrive at values that might reflect the maximum
potential exposure that could occur in different work situations where proper
protective gear was not utilized. Mean dermal exposure for all workers was 67.3 mg/hr
and the mean respiratory value was 0.62 mg/hr of work activity. Workers at the
bagging machine were subjected to greater exposure than mixers or workers who stacked
filled bags on pallets, operated the bag closing machine, or packed filled bags in
cardboard cartons for storage or shipment. Considerable variation was found in the
range of exposure values for each work activity. Such variations were thought to be
due to several factors, including worker carelessness and bagging equipment malfunction
the results indicated that workers in formulating plants exposed under conditions
similar to those found in the study should not become lax in the use of approved
protective gear on in following other safety precautions that prevent excess exposure
to highly toxic pesticides. Relatively high values obtained during a few of the test?
exposure,periods indicated that even minor lapses in adherence to safety precautions
ttight be sufficient to allow pftlWtfflft Afe B66tfSENT ANALYSIS
»r DESCRIPTORS
pesticides
safety
protection
environment
clothing
18. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
b.lDENTIFIERS/OPEN ENDED TERMS
Parathion
19. SECURITY CLASS (Tfus Report)
TTOPT ASSTVTPTl
20. SECURITY CLASS /This page I
UNCLASSIFIED
c. COSATI I-'icid/Group
06 F, Q
21. NO. OF PAGES
22. PRICE
Form 2220—1 (Rev. 4—77) PREVIOUS SCIT-ON is OESOL.ETE
18
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MS-77-079
TECHNICAL REPORT DATA
/Please read Inuruciions on the reverse before completing)
1. REPORT NO.
2.
3. RECIPIENT'S ACCESSION-NO.
4. TITLE AND SUBTITLE
5. REPORT DATE
SUBSTITUENT EFFECTS AND ADDITIVITY IN THE CARBON-13
NUCLEAR MAGNETIC RESONANCE SPECTRA OF CHLORINATED NAPHTHA'
T T7\rtrg A\TT> THiTjR CHLORINATED NAPHTHOL METABOLITES
6. PERFORMING ORGANIZATION CODE
JES AND
iTHORfS)
7. AUTHOR
8. PERFORMING ORGANIZATION REPORT NO
Nancy K. Wilson and Robert D. Zehr
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Environmental Toxicology Division
Health Effects Research Laboratory
Office of Research and Development
Research Triangle Park, N.C. 27711
10. PROGRAM ELEMENT NO.
1EA615
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
Health Effects Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, N.C. 27711
13. TYPE OF REPORT AND PERIOD COVERED
RTP,NC
14. SPONSORING AGENCY CODE
EPA 600/11
15. SUPPLEMENTARY NOTES
Published in: J. Org. Chem. 43(9):1768-1773, 1978
16. ABSTRACT
Carbon-13 and proton nuclear magnetic resonance spectra were obtained for
12 chlorinated naphthalenes and six chlorinated naphthols, some of which are metabo-
lites of the naphthalenes. The validity of the use of additivity of chlorine and
hydroxyl substituent effects to predict 13-C chemical shifts in these compounds was
examined. Deviations from the additivity predictions resulted from per and ortho
substituent interactions, both steric and hydrogen bonding. Despite these deviations,
additive substituent parameters could be used to assign 13~c spectra correctly
and to distinguish uniquely between similar isomers.
7.
KEY WORDS AND DOCUMENT ANALYSIS
DESCRIPTORS
b.lDENTIFIERS/OPEN ENDED TERMS C. COSATI field,Group
nuclear magnetic resonance
proton magnetic resonance
carbon isotopes
naphthalenes
naphthols
chemical analysis
Carbon-13
chlorinated naphthalenes
07, C
06, F
-L
3. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
j 19. SECURITY CLASS .-Tins Reporii \ 21. MO. OF PAGES
UNCLASSIFIED i
20. SECURITY CLASS tTMspaqe)
UNCLASSIFIED
22. PRICE
EPA .Form 2220-1 (9-73)
19
-------
TECHNICAL REPORT DATA
iP'i-atc read Instructions an the Kffru before completing '
1. REPORT NO.
4. TITLE AND SUBTITLE
THE IDENTIFICATION OF POLYC
TRACE LEVELS IN HUMAN ADIPO
7. AUTHORIS)
L.H. Wright, R.G. Lewis, H.
J M SimDcon
9.Tȣ'BIJORMIN'G ORGANIZATION NAME AH
Environmental Toxicology Di
Health Effects Research Lab
Office of Research and Deve
Research Triangle Park , N . C
12. SPONSORING AGENCY NAME AND ADC
Health Effects Research Lab
Office of Research and Deve
U.S. Environmental Protect!
Research Triangle Park, N.C
Z. , J. RECIPIENT'S ACCESSION NO.
5. REPORT DATE
HLORINATED TERFHENYLS AT 6 PERFORMING ORGANIZATION CODE
SE TISSUE BY GAS CHROMATOGRAI HY/
8. PERFORMING ORGANIZATION REPORT NO.
L. Crist, G.W. Sovocool, and
D ADDRESS 1O. PROGRAM ELEMENT NO.
vision 1EA615
oratorv n. CONTRACT/GRANT MO.
lopment
. 27711
RESS 13. TYPE OF REPORT AND PERIOD COVERED
oratory RTP.NC
looment 14~ SPONSORING AGENCY CODE
on Agency EPA 600/11
. 27711
15. SUPPLEMENTARY NOTES
Published in: Journal of Analytical Toxicology 2:76-79, 1978
16. A3STRACT
Pooled samples of human adipose extracts representative of the general
population of the United States were examined by gas chroma tography-mass spec trometry.
The use of gel permeation chrdmatography for cleanup was found to offer a significant
improvement in detection capability. In addition to previously documented organo-
chlorine residues, numerous polychlorinated terphenyls and three additional components
of technical chlordane were identified.
17.
a. DESCRIPTORS
chemical analysis
bioassay
tissue extracts
18, DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
KEY WORDS AND DOCUMENT ANALYSIS
b.lDENTIFIERS/OPEN ENDED TERMS' C. COSATI Held/Group
gel permeation 07 C
chroma tography
polychlorinated terphenyl s
19. SECURITY CLASS / This Report) 21. NO. OF PAGES
UNCLASSIFIED
20. SECURITV CLASS i This page/ 22. PRICE
UNCLASSIFIED
EPA Form 2220-1 (Rev. 4-77) PBEVIOOS ECITION 'S OBSOLETE
20
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