United States
             Environmental Protection

             Agency
             Office of
             Research and Development
             Washington D C 20460
April 1979
             Water
&EPA
Caribbean
Rum  Study

Effects of
Distillery Wastes
on the Marine Environment

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            REPORT TO CONGRESS








THE RUM INDUSTRY AND RUM DISTILLERY WASTES




  IN PUERTO RICO AND THE VIRGIN ISLANDS:




  EFFECTS ON THE MARINE ENVIRONMENT AND




            TREATMENT OPTIONS
                APRIL 1979
     95th Congress Conference Report




                No. 95-830








         To Accompany H.R.  3199
             Prepared by the




    Office of Research and Development



   U.S. Environmental Protection Agency

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\
?   UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
                  WASHINGTON, D.C. 20460
                                                  THE ADMINISTRATOR




                  LETTER  OF  TRANSMITTAL


Honorable Jennings  Randolph
Chairman, Committee on
   Environment and Public Works
United States Senate
Washington, D.C.  20510

Dear Mr. Chairman:

     The Statement  of  Managers accompanying the Clean Water

Act of 1977 directed the Environmental Protection Agency  to

conduct a study  to  determine if certain Caribbean rum

distillers might safely  dispose of natural wastes untreated

into the marine  environment.

     I am pleased to submit herewith the report on our

findings.
April 26, 1979             'Douglas M. Costle

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 \
w I   UNITED STATES£NVIRONMENTAL PROTECTION AGENCY
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        ATLANTIC  OCEAN
                                   N
                                   \
                 DOM.
                 REP
JAMAICA

   CARIBBEAN  SEA
                            VIRGIN
PUERTO
  RICO
       Map of the Caribbean

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                                  DISCLAIMER








    This report has been reviewed by the U.S. Environmental Protection




Agency and approved for publication.  Mention of trade names or commercial



products does not constitute endorsement or recommendation for use.

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                                                                        ii
                                   FOREWORD








    This report is being submitted to the Chairmen of the Senate Committee




on Environment and Public Works and the House Committee on Public Works and




Transportation in compliance with a Congressional request that the U.S.



Environmental Protection Agency study certain wastewater discharges




associated with the Caribbean rum distillers.  The study request was




included in the Statement of Managers as part of the Conference Report (No.



95-830, p. 113) accompanying the 1977 amendments to the Clean Water Act.




This report summarizes the Agency's research efforts in response to the




Congressional request.  Detailed discussions of the studies are included in




appendices to this report.

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                                                                        iii
                                   ABSTRACT








    The objectives of this study, as stipulated by the Congress, are:  (1)




to determine if untreated wastes from rum distilleries in Puerto Rico and




the Virgin Islands are harmful to the receiving waters to which they are




presently discharged;  (2) to determine if these wastes can be discharged to



the marine environment for some beneficial purpose, such as aquaculture; and




(3) to examine technologies that might facilitate the use of the nutrients




in these wastes, or might reduce the discharge to the marine environment.



    Studies of the receiving waters at three Puerto Rico and Virgin Islands




rum distillery sites demonstrated that the wastes as presently discharged




are not beneficial to organisms in the receiving waters.  Organisms studied



in both the field and the laboratory were adversely affected when exposed to




a wide range of concentrations of the wastes.  A wastewater analysis showed




the effluent to be very high in biochemical oxygen demand and total solids,



low in pH, low in inorganic nutrients and to contain several compounds




identified by Congress as toxic  (priority) pollutants  in enacting the 1977




Clean Water Act.  Under Section 307(a)(1) of the Act,  EPA must  control toxic



pollutants through the application of best available technology.




    A study of technologies for the treatment  and utilization of the wastes




included land disposal, ocean discharge, evaporation,  biological treatment,




aquaculture, and process modifications. Land disposal  is believed to be  a




promising option for two of the distilleries studied,  Puerto Rico Distillers




(PRO) and Virgin Islands Rum Industries Ltd. (VIRIL).

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                                                                     iv
Evaporation of the effluent, resulting in a potentially usable by-product,



CMS (condensed molasses solubles), is a viable option although the CMS




market is uncertain.  Biological treatment, in a system comprising an




anaerobic and an aerobic phase, was found technologically feasible to



achieve pollution abatement.  Color, however, remains substantially




unaffected.  Pilot plants for evaporation at Bacardi's Catano plant and




biological treatment at PRD are now in operation and under study.




Aquaculture was found not to be a promising option.  Ocean disposal and




discharge to a POTW were determined to be unacceptable.




    An economic study provided a profile of the industry and its relation to




the economies of Puerto Rico and Virgin Islands, and an analysis of the rum




market.  The rum industry provides a sizeable and growing share of general




fund revenues in both Puerto Rico (12.7J in 1978) and the U.S. Virgin



Islands (16.MJ in 1978).  The degree to which wastewater treatment would




affect the industry as a whole and the individual plants is dependent on




many variables, including cost of treatment methods selected, continuation



of the present tax structure and continuation of sales growth.  The study




further indicated that rum sales are presently rising and are projected to




continue to do so for the next decade.  The resulting increase in rum



production is expected to cause a corresponding increase in effluent loads.




    While the report provides information on several available methods of




rum wastewater treatment, it remains the responsibility of the individual



distillers to select a treatment which they will employ to reduce pollution




to levels acceptable under the Clean Water Act.

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                              TABLE OF CONTENTS

Disclaimer	.
Foreword	   ii
Abstract	ill
List of Illustrations	   vi
List of Tables	vii
Acknowledgment	viii
    1.  Study Background and Design  	     1
    2.  Conclusions  	     5
    3.  Rum Industry Characteristics 	     6
         Introduction and Summary  	     6
         Industry Structure  	     7
         Process Description 	   14
         Wastewater Characterization 	   17
         Pollutant Reduction Through Process Modification  ....   20
    U.  Effects of Rum Wastewaters on Marine Ecosystems  	   25
         Introduction and Summary  	   25
         Puerto Rico Distillers  	   27
         Bacardi Corporation 	   39
         Virgin Islands Rum Industries Ltd	   42
         Bioassay Experiments  	   46
         Discussion of Tropical Marine Ecosystems and
           Organic Effluents 	   55
    5.  Waste Treatment and Utilization Technologies 	   58
         Introduction and Summary  	   60
         Disposal to a POTW	   60
         Aquaculture	   61
         Ocean Disposal	   62
         Disposal by Land Application	   64
         Evaporation, with CMS By-product   	   68
         Biological Treatment  	   72
    6.  Economic Assessment  	   76
         Introduction and Summary	   76
         Relationship of the Rum Industry to the Economies
               of Puerto Rico and the Virgin Islands	   77
         Economic Implications of Wastewater Treatment 	   79
References	   86

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                                                                     vi
List of Illustrations                                                 Page

    Figure
              Map of Caribbean
          1   Graph, rum production, 1960-1978  	   8
          2   Graph, rum as % of total liquor consumption 	   9
          3   Map, showing locations of distilleries  	  10
          4   Flow diagram of a typical distillery	  15
          5   Map of Arecibo, PRO site	30
          6   Photo of Arecibo, PRD Site	31
          7   Close-up of PRD discharge pipe		32
          8   Photo of stained surf at Arecibo, west of discharge
               point	32
          9   Photo of Bacardi plant at Catano, aerial  	  44
         10   Map of Catano area, Bacardi plant site	45
         11   Photo of Bacardi outfall structure, Ensenada
               de Boca Vieja	45
         12   Map of St. Croix, V.I., VIRIL site	47
         13   Photo of outfall and plume from VIRIL discharge
               site	48
         14   Map, location of tidepools studied at Arecibo 	  51
         15   Graph, survival of transplanted Echinometra lucunter
               in tidepools at Arecibo with and without effluent
               discharge	55
         16   Graph, bioassay of mosto in seawater:  survival of
               Echinometra lucunter    	  57
         17   Graph, bioassay of mosto in seawater:  survival of
               Chiton squamosus	57
         18   Graph, bioassay of mosto in seawater:  effect
               on righting behavior of Echinometra luounter 	  58
         19   Graph, bioassay of mosto in seawater:  survival of
               Brachidontes exustus 	  58
         20   Graph, bioassay of mosto in seawater:  survival of
               Callinectes sapidus  	  59
         21   Graph, bioassay of mosto in seawater:  effect on
               byssal thread production by the mussel Brachidontes
               exustus	  60
         22   Photo of Serralles plant surrounded by cane fields  .  .  72
         23   Photo of Anamet pilot plant in operation at PRD ....  81
         24   Diagram of Anamet system  	  81

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                                                                     Vll
List of Tables                                                       Page
    Table 1   Estimated operating levels of rum distilleries
               and other statistical information  	   13
          2   Priority pollutants in mosto and combined
               effluent	19
          3   Mosto from three distillers:  conventional
               parameters	21
          4   Intertidal samples taken at the discharge site at
               Arecibo before and after resumption of mosto
               discharge	32
          5   Intertidal samples at an upstream control station
               at Arecibo before and after resumption of mosto
               discharge	33
          6   Tidepool fish species observed on selected dates
               at Arecibo	34
          7   Biochemical oxygen demand at selected distances from
               effluent outfall in coastal waters at Arecibo on
               various dates  	   37
          8   Chemical oxygen demand at selected distances from
               effluent outfall in coastal waters at Arecibo on
               various dates  	   38
          9   Survival of transplanted Echinometra lucunter in
               tidepools before resumption of effluent
               discharge	48
          10   Effects of effluent of dissolved oxygen levels in
               a tidepool at the PRD, site, Arecibo	48
          11   Largest possible capital investment for waste treatment
               with model plants remaining viable (no closure)   ...   84

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                                                                       Vlll
                               ACKNOWLEDGMENTS








    The cooperation of a number of individuals and organizations in Puerto




Rico and the Virgin Islands is gratefully acknowledged.   First,  the




assistance of Mr. Weems Clevenger of the EPA San Juan office was invaluable




in many aspects of the project.  Within the Puerto Rican government, special




thanks are extended to Mr. Julio Cesar, Secretary of the Treasury,  Mr.




Bertram Finn, Director, Office of Economic Research, Economic Development




Administration; and Mr. J.M. Mitchelhill, Assistant to the Executive




Director, Sugar Corporation of Puerto Rico.  Similarly,  in the Virgin




Islands government, thanks are due Mr. Henry Millin, Lt. Governor;  Mr.  John




Tinsley, Chief Economist, Department of Commerce; Mr. William Quetel,




Assistant Director, Industrial Development; Mr. Claude Christain, Department




of Finance; and Mr. William Blum, Department of Commerce.




    Considerable help also was given by rum industry personnel.  Within the




Bacardi Corporation, thanks are due Mr. Alfred O'Hara, President, and Mr.




Richard Reiss, Vice-President and General Manager.  Dr. George Dorion,




Director of Advanced Projects, was particularly helpful in arranging for




visits to the Bacardi San Juan Plant and in providing information and




technical data.  Likewise, Mr. Felix Serralles, Jr., arranged for a most




instructive tour of the Destilleria Serralles facilities for EPA personnel.




Also helpful were Dr. Herminio Brau, President of Puerto Rico Distillers,




Inc. and Messrs. Cedric Nelthropp and Milton Seasonwein of Virgin Islands




Rum Industries, Ltd.  Mr. Carlos Yordan, Executive  Secretary of the Puerto




Rico Rum Producers Association, Inc.,  also has been most helpful.




Photographs in this report were taken  by E. Zell Steever.

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                                  SECTION 1




                         STUDY BACKGROUND AND DESIGN








    Section 304(b)(1)(A) of the Federal Water Pollution Control Act




Amendments of 1972, (P.L. 92-500) required the U.S. Environmental Protection




Agency (EPA) to establish effluent limitations guidelines for categories of




industrial point sources.  Guidelines have not been promulgated for




distillers of rum in Puerto Rico and the Virgin Islands because sufficient




information specific to Caribbean cane molasses rum production was not




available to formulate a guidelines subcategory.  In the absence of




guidelines, National Pollutant Discharge Elimination System (NPDES) permits




were issued to Caribbean distillers by EPA in 1974 and 1975 based upon "best




engineering judgment" under authority of section 402(a)(1) of the FWPCA.




One distiller, Bacardi Corporation, accepted its NPDES permit; the other




distillers, Puerto Rico Distillers, Inc. (PRO) and Schenley Industries,




{operator of Virgin Islands Rum Industries Ltd. (VIRIL)l challenged the




terms of their permits and requested adjudicatory hearings.




    A consolidated hearing was convened in July 1977 and adjourned after two




weeks of testimony.  Although the hearing has not been reconvened, EPA and




the rum distillers have continued negotiations toward a resolution of the




controversy over effluent limitations.  As part of the negotiation process,




EPA established a Technical Committee in August 1978, composed of technical




experts from EPA and the rum industry, for the purpose of reaching a




consensus on the issue of available alternatives for the treatment of rum




wastes.



    The issue of treatment requirements for the Caribbean rum industry also




was raised before the House Committee on Public Works and Transportation as

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part of deliberations on 1977 amendments to the Federal Water Pollution

Control Act. Consequently, the Statement of Managers in the Conference

Report accompanying these amendments, subsequently adopted as the Clean

Water Act of 1977 (P.L. 95-217), contained the following provision:

    It has been stated that some Virgin Islands and Puerto Rico rum
    distillers might safely dispose of certain natural  wastes
    untreated into the marine environment.  In response to these
    statements, the conferees direct the Administrator to conduct a
    study, to be completed by January 1979, to ascertain if there is
    merit in this argument and if disposal can be environmentally
    acceptable or even possibly beneficial.  In this study the
    Administrator should specifically examine geographical,
    hydrological and biological characteristics of marine waters
    receiving such wastes to determine if the discharge can be
    environmentally acceptable either for the purpose  of aquaculture  or
    some other purpose.  In addition, the study should examine
    technologies which might be used in these industries to facilitate
    the utilization of the valuable  nutrients in  thes|swastes or  the
    reduction  in discharge to  the marine  environment.


    To comply  with this  Congressional request,  EPA  organized a  research

effort in which Agency scientists and non-governmental technical  and

scientific  consultants participated.  The research  tasks were as  follows:
 •It  is  assumed  that  the  term  "organic wastes"  was  intended here.   The  term
 "natural wastes"  is  generally understood  to  designate  substances  that  enter
 the  environment as a result of  "natural"  processes,  or those that are  not
 the  result of human  industry  or commerce.  The distillery discharge is an
 industrial effluent  composed  mainly  of  organic by-products of fermentation
 and  distillation.

 "House Conference Report  No. 95-830 accompanying H.R.  3199, 95th Congress,
 1st  Session, p. 113, reprinted  in  A  Legislative History of The Clean Water
 Act  of  1977, Vol. 3, p.  297,  Senate  Committee  on Environment and  Public
 Work¥7  35TE Congress,  2d Session (1978).

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    1.  A study of the geographical, biological and hydrological

characteristics of the marine environment at two distilleries in Puerto Rico

and one distillery in the Virgin Islands, and an assessment of the effects

of the effluent discharge on the marine environment (Section 4 of this

Report).  The study was conducted by:
    The Marine Ecology Division at the Center for Energy and
        Environment Research (CEER), affiliated with the University of
        Puerto Rico and the U.S. Energy Research and Development Agency.
        CEER laboratory at Mayaguez, P. R.  Dr. Juan G. Gonzalez, Project
        Leader, under an interagency agreement between EPA and the U.S.
        Department of Energy.  #IAG-78-D-X0225
    Report:  "Biological Effects of Rum Slops in the Marine Environment"
        (1979)
    Short title:  Puerto Hico Report

    The Virgin Islands Environmental Research Service at the
        College of the Virgin Islands, St. Thomas and St. Johns.  Dr.
        Michael J. Canoy, Project Leader, under an EPA grant.  #806110
    Report:  "Environmental Effects and Acceptability of Effluents from Rum
        Distilleries" (1979)
    Short Title:  Virgin Islands Report

    2.  An analysis of rum slops (mosto in Spanish), other waste stream

components, and total combined waste stream at each of three distilleries

(Section 3 of this report).  This analysis was performed by:

    EPA Region IV, Surveillance and Analysis Division (SAD), Athens,
         GA. Mr. Steve Hall, Project Leader.
    Report:  "Rum Wastewater Characterization Study, Puerto Rico and Virgin
         Islands, July 17-22, 197B" (1979)
    Short Title:  SAD Wastewater Study

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    3.  An evaluation of the various waste treatment, by-product recovery

and waste utilization alternatives (Section 5 of this Report).  This

evaluation was conducted by:

    Stearns, Conrad and Schmidt, Consulting Engineers, Inc.
        (SCS), Long Beach, CA., Mr. Kenneth LaConde, Principal Investigator,
        on contract to EPA.
    Report:  "Study of Rum Distillery Waste Treatment and By-product
        Recovery Technologies"  (1979)
    Short Title:  SCS Technology Study


    4.  A study of the rum market structure and, through economic modelling,

the financial impact of effluent controls  (Section 6 of this  Report).  This

study was conducted by:
    Development Planning and Research Associates  (DPRA),
         Manhattan, KS.  Mr. Richard Seltzer, Principal Investigator, on
         contract  to EPA.
    Report:   "Economic Impact of Effluent Regulations  on  the Rum Industry
         (Puerto Rico and Virgin Islands)"  (1979)
    Short title:   DPRA Economic Study
    The research efforts were coordinated by the EPA Office of Research and

Development, Environmental Research Laboratory, Narragansett, R.I.,  E. Zell

Steever, Project Coordinator; and the EPA Office of Water Enforcement,

Washington, D.C., Harry Thron, Project Coordinator.

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                                SECTION 2

                               CONCLUSIONS



    This study was designed to address the questions posed by the

Congress.  EPA believes this report adequately responds to those

questions.

    The study was not intended to be a definitive exploration of all

issues relating to effluent discharges by the rum distilling industry.

The investigations, nonetheless, provided a great deal of information on

the nature of rum wastewaters, effects of these wastewaters on the marine

environment, and treatment, reuse and  disposal alternatives.  This

information has led to the  following conclusions:
    1.  Chemical analyses  of  rum distillery  effluents  confirmed  the
        presence of high concentrations  of organic materials  and the
        presence of heavy  metals and  some toxic (priority)  pollutants.

    2.  Direct discharge of rum distillery effluents as  presently
        practiced  is  harmful  to the marine environment.

    3.  Aquaculture/mariculture is not a feasible alternative for rum
        waste treatment  or utilization at the present  time.

    4.  Addition of mosto  to  the "nutrient-poor" marine  ecosystems of the
        Caribbean  considered  in this  study  does not  improve water quality.

    5.  Technically  feasible  alternatives are available  for waste
        treatment  or  disposal of rum distillery effluents.

    6.  There  are  technically feasible effluent treatment or disposal
        alternatives  that  are economically affordable.

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                                  SECTION 3




                         RUM INDUSTRY CHARACTERISTICS








INTRODUCTION




    This section describes the present structure of the rum industry, the




process of rum production, the composition of the three distilleries'




wastewater streams and their major components, and some possible




modifications in the rum production process that can result in pollution



reduction.








Summary:




    The U.S. Caribbean rum industry has grown markedly during the past two




decades, although the number of distillers has declined to a total of five,




operating six plants.  The growth in demand for rum, and hence of




production, continues today, as rum's share in the total U.S. liquor market




continues to expand.




    Rum manufacture is a process of fermentation and several distillation




steps, in which wastes are generated from a number of sources.   The mosto




stream from the main still,  or alcohol stripping column,  contains nearly all




(over 95$) of the pollution load.




    A wastewater analysis revealed the presence in the effluent of toxic




(priority) pollutants, which appear to be a less serious problem than the




very high levels of organic compounds and attendant high biochemical oxygen




demand (BOD ) and chemical oxygen  demand (COD).




    Certain modifications in the rum production process can reduce pollutant



levels in the effluent.

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Outline:

    Industry Structure

    Process Description

    Wastewater Characterization
        Priority Pollutants
        Conventional Pollutants

    Pollutant Reduction Through Process Modifications


INDUSTRY STRUCTURE1

    During the past two decades the U.S. Caribbean rum industry has

experienced significant expansion and change (Fig. 1).  In the early 1960s,

rum production moved from a period of stability to one of appreciable

growth.  Competition among the distilleries was strong, and the more

efficient ones increased their production markedly.  By the end of the

decade, the industry had become dominated by a small number of comparatively

large distilleries.  The demand for rum continued to grow in the 1970s,

along with a steady increase in rum's share in the U.S. liquor market (Fig.

2).

    The Puerto Rico and U.S. Virgin Islands rum industry presently consists

of five distillers.  These distillers, from largest to smallest in terms of

annual rum production are listed below and are located on the accompanying

map (Fig. 3):
Bacardi Corporation (2 plants)

Puerto Rico Distilleries, Inc. (PRO)

Destilleria Serralles, Inc.

Virgin Islands Rum Industries, Ltd.
   (VIRIL)

Brugal and Company
San Juan (Catano), Puerto Rico

Arecibo, Puerto Rico

Ponce (Mercedita), Puerto Rico

Fredericksted, St. Croix,
  U.S. Virgin Islands

Fredericksted, St. Croix,
  U.S. Virgin Islands.

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               28
               24
            CO

            O
               20
            U- ,e
            O I6
            O
            DC
            a.
               12

            O
            -I
            d  8
PUERTO RICO

RUM PRODUCTION
                        VIRGIN  ISLANDS

                        RUM  PRODUCTION
                     I  i   i
                        t   I  I   I  I  I
               I960   62   64   66   68   70

                                  YEAR
                       72   74    76
Figure 1.  Hum Production,  1960-1978
* A proof gallon of rum is defined as one gallon of rum at  100 proof (50?
alcohol).  Based on the commonly sold fifth bottle of rum at 80 proof, 6.25
bottles of rum equal 1 proof gallon.
Source:   DPRA Economic Study

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          RUM  AS % OF U.S. LIQUOR  CONSUMPTION
IU
UJ
1 8
o
_J
? 6
0
1-
u_
0 4
UJ
O r>
a: 
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                                                     10
          (PRO)
             ATLANTIC   OCEAN
                   (BACARDI, OLD SAN JUAN)
X.  /(BACARDI)
            ARECIBO

          PUERTO  RICO
                                                   A
                                                  N
                                           SAINT
                                           THOMAS
                                             VIRGIN  ISLANDS
                (SERRALLES)
        CARIBBEAN  SEA
                    (BRUGAL)
                              (VIRIL)
                                MILES
                             0        30
                               i      il
                             SAINT CROIX
 DISTILLERY  SITES
         0     35
         KILOMETERS
Figure 3. Map, showing locations of distilleries in the U.S. Caribbean.

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                                                                     11




    Bacardi operates two separate distilleries.  The main distillery in


Catano, P.R., has been operating since  194*1.  The newly acquired (July 31,


1978) Old San Juan Distillery was previously owned by Schenley Industries.


Both facilities discharge their untreated wastewaters into the Ensenada de


Boca Vieja estuary.


    PM) has operated its distillery  in  Arecibo  since the late 1800s.  At


present untreated wastewater is discharged directly to the beach and thence


to the Atlantic Ocean.


    Destilleria Serralles is adjacent to  the Serralles family's Mercedita


sugar cane fields near Ponce, P.R.   Since 1935,  Serralles has disposed of


its wastewater by applying it, diluted  with  fresh irrigation water, to the


cane fields and other crops.  A small volume of the wastewater entered the


nearby Inabon River until 1977 when  the distillery upgraded its wastewater


control program by installing a closed  loop  "no discharge" land application


system.  Presently, Serralles discharges  no  wastewater into any body of

                                        2
water and thus requires no NPDES permit.


    The VIRIL distillery on St. Croix,  V.I.  discharges its wastewater  to  a


former regional sewer duct which conveys  the untreated plant effluent  to  the


Caribbean Sea and discharges it about 600 m  offshore in about 6 m of water.


    The smallest rum distillery, Brugal,  is  also on St. Croix, and  trucks


its wastewater to the publicly owned treatment  works  (POTW) plant where it


is discharged untreated with the treated  effluent from the POTW to  the


Caribbean Sea via an outfall pipe 2700  m  long.

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                                                                     12


    In addition to these six distilleries, at least four plants rectify,

blend, bottle, and/or sell rum.  These non-distilling operations purchase

rum from the distilleries.

    Of the five distilling firms in Puerto Rico and the Virgin Islands,

three are owned by publicly traded corporations and two are privately

owned.  Serralles is owned by the Serralles family and Brugal by the Brugal

family.  (Brugal operates two other distilleries in Santo Domingo.)

    PRD is owned by the Seagram Company, the world's largest distiller,

which operates plants in the United States, Canada, and other countries.

VIRIL is owned by Schenley Industries which, in turn, is owned by

Rapid-American, a diversified corporation with interests in retail

merchandising, importation  and sale  of  alcoholic  beverages as well as  the

manufacture and sale of men's apparel and other soft goods.

    Bacardi Corporation is a publicly owned company  traded on  the

over-the-counter market.  The company distills, rectifies, and bottles  rum

in Puerto Rico.  Its wholly-owned subsidiary, Castleton Beverage

Corporation, bottles rum in Jacksonville, Florida.

    Firms in the rum industry typically  are not diversified.  While the

owning families or parent corporations may be involved in nonrelated

industries, most firms  in the rum industry are involved only  in the

manufacture and sale of alcohol and alcoholic beverages.

    Some distilleries currently operate  at less than maximum  capacity

(mostly by operating on only a  limited number of  days per year) while  others

operate at near capacity.   The estimated annual  production figures  (and

other statistics) for each distiller  are presented in Table  1.
 •Capacity  is  estimated  since  exact  data  were  not  available from the
 distillers.

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                                                                                               13
TABLE 1:   Estimated operating levels and other statistics for Puerto Rico and Virgin Islands rum
          distilleries,  1977-1978
Estimated
Run Production
Million Proof
Company 8t Location Gals/Year
Bacardi, P.R. 18. U
Bacardi, P.R.
(former Schenley) 	
Puerto Rico Distillers,
P.R. t.8
Distillerla Serralles,
P.R. 3.8
VIRH, (Sohenley), V.I. 3.1*
Brugal & Co., V.I. 0.2
Proof
Gals/ Day
60, 000
	
25,000
15,000
12,500
800
Estimated
Operating
Days/Year
300
	
200-250
250
200
	
Estimated
Plant Site
Land (Acres)
125
	
16
4,000*
25
	
Estimated Gallons
Volume of
Hosto/day
300,000
60,000
200,000
150,000
65,000
5,000
•Includes Serralles sugar cane fields surrounding distillery

Source:  Information supplied to U.S. EPA in verbal communications  from rum  industry personnel.  See also
Puerto Rican Rum Producers' Association 1977-78 Report and Alcohol  Control Board data, Government of the
Virgin Islands, 1977-78.

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                                                                      14


PROCESS DESCRIPTION3

    Rum is made by fermenting a dilute solution of cane molasses with yeast

for one to two days and then distilling the fermentation "beer" to recover

ethyl alcohol.  Molasses, also known as "Blackstrap" cane molasses, a

by-product of the cane sugar industry, is purchased by the distillers and

stored in large holding tanks prior to use.  The molasses is then pumped to

a tank or vat where phosphorus and nitrogen are added to satisfy the

nutritional requirements of yeast in fermentation.  To eliminate the

possibility of undesirable bacteria growth, the pH is adjusted to between 4

and 5 through the addition of sulfuric acid and the molasses is diluted with

water to about 22° Brix» (range 18° to 24°), a sugar concentration of

about 12$.

    The fermentation  process begins as the yeast  first  converts the sucrose

into equal parts of glucose and fructose.  These  in turn  are converted into

alcohol and carbon dioxide.  The  fermentation process yields a waste  stream

primarily made up of  yeast residues, sometimes referred to as fermenter

sludge.  The resulting fermentation product, known as "beer" or mash  (&% to

12$ alcohol), is then fed continuously through a multi-column distillation

process to separate the ethyl alcohol from the spent molasses and

fermentation by-products.  A typical distillery arrangement is shown  in

Figure 4.

    The first distillation column—the alcohol stripping  column—is operated

continuously for as long as six months, until it must be  cleansed of  the
*A measurement for sugar solutions  that  expresses,  on  the basis  of specific
gravity, the total solids content.

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                                                        15
                    FLOW DIAGRAM
             A  TYPICAL RUM DISTILLERY
  MOLASSES,

ADDITIVES.
                    WATER
                    YEAST
                  FERMENTATION
                     TANK
  ALCOHOL STRIPPING        ALDEHYDE
        COLUMN            COLUMN
              CONDENSOR
                                       WASTE
                                        RECTIFYING
                                          COLUMN
                                              PRODUCT

                                           95%  ETHYL ALCOHOL
                                           (ETOH) TO OAK BARRELS
                                           FOR AGING  1-3 YEARS
                                           j 'IUWKU.IUMU..|4*HP3W*««W^>I



                                           HEAVY
                                   SS*:<*^  ~*    KS&- /-I
                                   FUSEL OILS!
                                   ««IBife^^-'> •>. "-^IBilKMl
Figure 4.

Source:  SAD Wastewater Study

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                                                                      16






encrustations or "scale" that, in time, clog the passages  (or bubble  caps)




between chambers in the distilling column.  The heat applied at the bottom




of the column causes the ethyl alcohol (ETOH) and other volatile products to




rise as vapor into the condenser above the column, where on cooling they




liquefy and are collected for further refining.  This  stripping process




yields the major waste component in the rum production process, the "slops"




or mosto.




    The second still, ordinarily, is the aldehyde column.  Here ketones,




aldehydes, low boiling esters—the more volatile or lower  boiling fractions



in the alcohol, called "heads"—are collected and removed.  These heads may




be redistilled to recover whatever alcohol remains.  Since the heads  are




combustible, they may be recovered and used as boiler  fuel.   (This  is




presently the case with VIRIL and PRO).




    The product is drawn off  the bottom of the aldehyde  column and  sent on




to the third still, or rectifying column, where the  fusel  oils and  amyl




alcohols are removed.  These waste products may become part of the  waste




stream, or may be recovered and used as solvents or  fuels. Other




distillation columns may be employed to further refine the product.




    After the fermentation/distillation/rectifying process—which varies




from one distillery to another—the final product is aged  in  oak casks for




one to three years.  Finally, it is filtered, diluted, blended, bottled and



sold.

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                                                                     17


WASTEWATER CHARACTERIZATION14



    In rum production,  waste streams are generated from a number of

sources:   the fermentation tank (yeast heal), alcohol stripping column

(slops or mosto),  aldehyde column (heads), rectifying column  (fusel oils)

and other ancillary processes, such as cleaning, cooling and  boiler

blow-down (see Fig. 4,  above).  A general characterization of these waste

streams was undertaken to aid in the assessment of the various possible

waste treatment technologies and to better understand the impact of the

effluent on the marine environment.  Toxic (priority) pollutants and

conventional pollutants were considered  in this characterization.

    The Region IV EPA Surveillance and Analysis Division  (SAD) conducted the

surveys at Bacardi, PRD and VIRIL, taking samples at several  points of waste

generation at each plant on five consecutive  days.  Composite samples were

taken of the undiluted slops or mosto stream  and of the "combined  effluent"

where possible.   Single grab samples were collected from each facility's

raw process water, fermentation tank, aldehyde  column, and rectifying

column.  Flow rates of the wastewater streams also were determined.
 'Because the VIRIL total effluent  stream contains only a very small  amount
 (5% or less) of material other  than  the  slops  or mosto,  separate analyses
 were not performed on the VIRIL mosto  stream and final effluent stream.  The
 PRD final effluent contained a  significant  volume of cooling water.   The
 Bacardi waste streams were not  fully combined  at the outfall; refer  to the
 SAD Wastewater Study, figure 2, and  the  section entitled "Sampling Station
 Locations and Samples Collected,"  and  the entire document for further
 information on the wastewater analyses.

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                                                                     18






    Samples requiring immediate analysis were analyzed at the laboratory of



the University of Puerto Rico in San Juan.  All other chemical analyses,



excluding those for toxic organic (priority) pollutants, were performed by



the EPA, SAD laboratory in Athens, Georgia.  The organic compounds were



analyzed at West Coast Technical Services, Inc., laboratories in Cerritos,




California.








Toxic (Priority) Pollutants



    Several compounds included in the list of toxic materials (priority



pollutants) identified by Congress in the Clean Water Act were detected in



wastewaters at each facility.  These toxic (priority) pollutants were  found



in  the  raw process water supply, mosto  stream, fusel oil, aldehyde heads and



combined effluent  from each  facility.   The toxic  (priority)  pollutants found



in  the  mosto and combined effluent  streams are shown in Table 2.  The



effects of these substances  on the marine environment were  not directly




addressed in this  study.








Conventional Pollutants



    Of  all the waste  stream  sources  in  rum production,  the  mosto stream is



by  far  the most important; it  is  estimated  to constitute 65% of the  volume

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                                                                                                        19
                         TABLE 2:
Toxic (priority)  pollutants  in mosto and combined  effluent
                ug/1 = part/billion
                                       BACARDI
                                                                  PRO
                                                                                            VIRIL
                                Undiluted
                                  Mosto
           Combined1
           Effluent
          Undiluted
            Mosto
       Combined*
       Effluent
              Combined*
              Effluent
ORGANIC"

Phenol
Bis (2 ethylhexyl)  ph thai ate
r-BHC-Garane
1,1-di chloroethylene
Toluene
Carbon tetraohloride
1,1,1 trjchloroethene
Naphthalene
Benzene
Beptachlor epoxide
Dibenzo (a,h)  anthracene

INORGANIC
387 i 59
      10
               68
               10
             0.317

               10
              210

              252
               29
                  41
                  10
           0.07&0.36"»
                                10
             96
             10
                              10

                           0.316«««
               193
                                            110
                                             13
                                            140
                                            520
Sb
As
Be
Cd
Cr
Cu
Pb
Hg
Hi
Se
A«
Tl
Zn
Phenols (total)""
Cyanide
     12
     78
    190
   3850
   1110
    7.5
    600

     60

   2900
   1780
      3
  16
  85
 269
7200
1350
 4.6
 675

  60

2560
1080
   4
  16
  62
 470
5220
 940
   4
 830

  51

1170
 925
  14
 20

 10
 10
125
450
103
  4
 53
 10
 10
  7
  78
  77
   7
                                                            14
                                                            83
                                                           264
                                                          4080
                                                          1250
                                                           2.2
                                                          1040

                                                            58

                                                          4053
                                                          1025
                                                            15
 Source:  SAO Waatewater Study
 *  See footnote on p. 17.
 **  The analysis showed large amounts  of methylene chloride in many of the samples.  These readings, however, are
    thought to reflect laboratory use  of methylene chloride in performing the analyses rather than a feature of the rum
    effluent and thus are omitted from the table.
 »•• Hot confirmed by GC/MS.
 "••By the 4 AAP Procedure (Standard Methods).

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                                                                     20



                                                              5
of the rum effluent and to contain over 95% of the pollutants.   The exact


chemical composition of the organic fraction of mosto is uncertain, but it


is basically a mixture of sugar (estimated to be 5% to 8% by weight),


organic acids, amino acids, proteins, polysaccharides, and inorganic salt


complexes.  Analysis by gas chromotography/mass spectrometry (GC/MS)


revealed numerous oxygenated compounds, none of which is known to be highly


toxic.


    A comparison of the important characteristics of mosto for the  three


distillers under study is summarized in Table 3-  Although production


processes are quite similar, wastewater composition was  found  to vary


somewhat in composition from distillery to distillery and probably  also from


batch to batch.  Quality of molasses and  process  differences,  including


controls on Brix and distillation time, fermentation  time, additives used,


and heating and cooling methods account for  some  of this variation.


    Beyond these relatively minor differences, the analyses  reveal  very high


levels of organic material in the form of carbon  compounds and total solids


in the mosto streams of all three distilleries.  These factors contribute 'to


the high BOD /COD, which results in oxygen depletion  in  the  receiving


waters.  This characteristic appears to be the major problem associated with


rum effluents, and probably accounts for  much of the  damage  to marine


organisms observed in biological studies  discussed in Section  U.  The


amounts of nitrogen, phosphorus and other inorganic compounds  are comparable


to levels found in domestic wastes.
 POLLUTANT  REDUCTION THROUGH PROCESS MODIFICATION6


     A number  of production process modifications are  available  to  rum

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                                                                                                        21
TABLE 3:  Mosto stream from three distillers:   selected conventional pollutants:  5 day averages in mg/1.

Bacardi
PRO
TIB1L
BOD5
32,000
27,000
36,000
ODD
101,000
75,000
112,000
Total
Organic
Carbon
34,300
27,300
J»2,260
Organic
Nitrogen
859.0
550.0
952.0
Ammonia
Hitrogen
16.3
6.9
9.0
Phosphorous
138
118
119
Total
Suspended
Solids
6, « 00
4,700
9,700
Total
Solids
95,000
76,00
116,000
pH
1.8
4.7
5.1
 Source:  SAD Wastewater Study

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                                                                     22


distillers that could result in reductions of certain pollutants, improved

plant efficiency, water and energy conservation, recycling of waste

products, and recovery of other fermentation by-products.  Among the more

significant of such options are:

    1.  Pretreatment of molasses  prior to fermentation;
    2.  Removal and recovery of yeast from fermenter beer;
    3.  Removal of fermenter bottoms;
    U.  Recovery of fusel oil and "heads" as fuel supplements or other
        useful by-products;
    5.  Indirect heating of alcohol stripping column.


Pretreatment of Molasses

    Molasses pretreatment  is a well known technology in the distilling

industry.  The process clarifies and pasteurizes the raw molasses,  removing

gums, sludges, salts  and unwanted microorganisms—materials that tend to

inhibit  yeast fermentation and form  the "fermenter bottoms" that must be

removed  or become a part of the waste stream.

    Pretreatment is achieved by diluting the molasses, acidifying it with

sulfuric acid, and raising  the temperature almost to the boiling point.

Separation is generally carried out in settling tanks or by use of an

ejecting-type molasses clarifier.

    Pretreatment has important benefits.   Clarification and pasteurization

of the molasses yields a cleaner "beer" and alcohol; consequently, the

alcohol stripping column in the distillation process requires fewer

stoppages for descaling, and reduction in the build-up of sludges in this

column permits longer periods of operation.  Pretreatment does, however,

require additional energy and capital, and there is reportedly some loss of

fermentable sugars involved in the process.

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                                                                     23



    A partial pretreatment of molasses can be accomplished simply by


dilution and then centrifugation.  Without heating or acidification,


however, neither pasteurization of the molasses nor precipitation of calcium


salts is achieved.





Yeast Recovery
    Yeast can be eliminated from the fermenter beer before distillation by


centrifugation.  The centrifuged yeast may then be recycled for reuse in the


fermenters, sold for an animal feedstuff (with or without drying), or land


disposed.


    Another version of yeast recovery known as the Melle-Boinet technique


shortens fermentation time to 14 hours (from 24 to 72 hours), with up to 5%


increase in alcohol yield.  With this recycling technique, a high yeast


concentration is maintained in the fermenter and  cell reproduction is


reduced, thus allowing sugars previously used  for yeast growth to be


converted into alcohol.  With proper care, the yeast can be recycled up to

                                                       7
30 times before an appreciable loss in activity occurs.   This method can


only be used where molasses has first been pasteurized and pretreated to


remove components which inhibit the yeast.





Removal of Fermenter Bottoms


    Fermenter bottoms are the unfermentable solids and dead yeast


cells—"heal of yeast"—which settle to the bottom of a fermentation tank


and form sludge.  When fermentation ceases, the liquid or "beer"  containing


the live yeast cells, alcohol and spent molasses  is drained off for possible


yeast recovery and distillation.  Sludge must be  washed out of the tanks


before the next fermentation cycle.

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    Eliminating suspended inorganic and organic materials  (other than the



live yeast) which could become part of the rum wastewater  discharge can be




accomplished by the removal of fermenter bottoms as well as  by molasses



pretreatment.  It has been the practice at many distilleries to discharge



this sludge to the local sewer drain.  An alternative is removal by using a



 sludge pump to a holding tank and then land disposal.  The advantages of



 removal of fermenter bottoms over molasses pretreatment are  reported to be



 lower capital and  operating  (energy) costs and higher alcohol  yields.   Even



 higher yields, however, can  probably be realized when yeast  recycling is



 practiced along with molasses pretreatment.








 Recovery of Fusel  Oils and "Heads"



     Fusel oils and aldehyde  heads may be removed from the  distilling columns



 to a storage tank  and then burned in the  plant's boiler system.



     Both VIRIL and PRD already recover fusel  oil  and  heads and burn them as



fuel  on a continuous  basis.  Bacardi expects  to  install  a  recovery system by



the end of this  year.   Fusel oils may  be  recovered and marketed as solvents.








Indirect Heating of Alcohol Stripping  Column



    When heat  is supplied  internally by direct steam  injection into the



bottom of the  column,  condensed steam  (water) from this  heating method will



add 15$  to 20$ to  the  mosto volume.  This increased volume will result in



higher design  flow values  and, consequently, higher treatment costs,



particularly for evaporation.  External indirect heating through heat



exchangers avoids  adding this condensate  to the waste stream.  Indirect



heating  systems  are in operation currently at the VIRIL  plant.

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                                                                     25
                                  SECTION 4




                          EFFECTS OF RUM WASTEWATERS




                             ON MARINE ECOSYSTEMS
INTRODUCTION




    The rum distillers of Puerto Rico and the U.S. Virgin Islands have been




discharging untreated wastes directly into the nearshore marine environment




for many years.  The effluent is a spent molasses-like mixture with a high




organic content, high BOD,-, unfermented sugars, fermentation by-products,




organic acids, and various inorganic materials (Section 3).  At each site




the effluent forms a plume that follows the prevailing current and is




clearly visible for one to six kilometers downstream from the point of




outfall.




    Studies were conducted in Puerto Rico and in the Virgin Islands




(beginning in May 1978 and substantially completed in September 1978) on the




effects of this effluent on the marine environment.  In each case, one




distillery site was selected, at which the near field effect of the rum




distillery discharge was the focus of the research.  In Puerto Rico, the




main criterion for selection was the relative absence of other sources of




pollution near the site; thus the PRO plant site at Arecibo was indicated.




Abbreviated field studies also were carried out at the Bacardi outfall




site.  In the Virgin Islands, the site of the VIRIL plant, the Islands' only




sizeable distillery, was studied.




    The research teams in Puerto Rico and the Virgin Islands undertook field




studies to determine the design for a series of field and laboratory




bioassay experiments through which the effects of the effluent on the marine

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                                                                     26


community could be assessed.  The time frame did not allow for prior

gathering and analysis of baseline information or for evaluating seasonal

variations.  Study designs and methods are detailed in the separate

     ,  8,9
reports. '



Summary:

    Observations at the three distillery sites indicated that the rum

effluents as presently discharged are harmful to the marine environment.

Certain  indigenous organisms were absent or rare in the presence of the

discharge.  These observations were confirmed by field and laboratory

bioassays,  in which two selected test organisms, the sea urchin Echinometra

lucunter and the  "coat-of-mail" shellfish Chiton squamosus evidenced

mortality  and altered  behavior at the lowest mosto concentration tested,

.01$,  or a  dilution of 1:10,000.  Two other  organisms, the mussel

Brachidontes exustus and the crab Callinectes sapidus, were more resistant

to the mosto in laboratory tests, and survived in dilutions of 1:200.  In

field bioassays, however,  most of the crabs confined in the effluent plume

died, probably as a result of oxygen depletion in the water column and the

anoxic (without oxygen) condition of bottom sediments, related to the high

BOD5/COD of the effluent.


Outline:

Puerto Rico Distillers:  Arecibo and the Atlantic Ocean
    Site Description
    Field Studies
Bacardi:  Catano and the Ensenada de Boca Vieja
    Site Description
VIRIL:  St. Croix and  the Caribbean Sea
    Site Description
    Field Studies
Bioassay Experiments
    Field Bioassays
    Laboratory Bioassays
Discussion of Tropical Marine Ecosystems and Organic Effluents

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                                                                     27



PUERTO RICO DISTILLERS:  ARECIBO AND THE ATLANTIC OCEAN10


Site Description


    The PRD distillery at Arecibo, (Fig. 5), unlike the Bacardi plant at


Catano, is relatively free of other sources of pollution.  (A raw sewage


outfall and small fresh water influents east and upcurrent of the PRD plant


are exceptions).  Control or "reference" areas were established as follows:


(1) about six kilometers east of PRD at Islote, a previously well-studied


area where conditions are similar in most respects; and  (2) downstream


beyond the influence of the plume.


    The distillery is situated on an eroded cliff  (Fig.  6) overlooking a

                                                       *
rocky sand beach.  PRD discharges about 200,000 gallons  of mosto a day


during its 200 to 250 days of annual operation.  At the  beginning of the


study period, May 9, 1978, no mosto was being  discharged,  nor had any been


discharged for about 6 weeks.  Mosto discharge commenced on about May 23,


and was intermittent through June 6; from then until July  3, discharge was


sustained.  There was another interruption until July  12*,  and discharge was


again sustained after this point.  The hot, acidic effluent falls to the


beach below from a large pipe protruding from the  cliff  face (Fig. 7).  A


dark brown and white foaming "mosto river" meanders down the beach and


empties into the surf.  There is a pervasive odor  of burnt molasses with a


suggestion of hydrogen sulfide.  A strong prevailing alongshore current


generally carries the effluent plume to the west (Fig. 8).  There are


occasional reversals when the plume swings  around  180  degrees and hugs the


shore to the east.  Strong wave action mixes the effluent  with sea water,


which turns a dark reddish-brown and becomes opaque.
 Since rum and rum effluents are normally referenced in gallons, this
denomination is used in preference  to the metric equivalent.

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                                                     28
  OBRERO
             ATLANTIC   OCEAN
             PUERTO /
               RICO ^
            DISTILLERS
         I Km
          Mile
                                  PUERTO
                                DE ARECIBO /
Figure 5. Map of Arecibo, P.R.

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                                                                     29
Figure 6.  A low altitude aerial photograph of the PRD plant at Arecibo,
           P.R.  The effluent is discharged onto the beach from a pipe
           protruding from the eroded cliff.  The meandering effluent stream
           enters the surf zone to the west, creating a dark brown plume
           which flows in a westerly direction downstream with the
           prevailing alongshore currents.

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                                                                                  30
Figure 7.   A close up  of the  rum effluent  discharge from the PRD plant onto
           the beach at  Arecibo,  P.R.,  looking west.
Figure 8.  Mosto-stained surf downstream (west) from the discharge point for
           the PRD plant at Arecibo, P.R.,  looking north.

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                                                                     31






Field Studies




    An initial field reconnaissance revealed certain conditions that would




control and limit the design of the research effort at Arecibo.  These




conditions are:  (1) the plume hugs the coastline and moves generally in a




westerly direction; (2) the effluent is discharged to a prevailing heavy




surf, coupled with a constant onshore trade wind blowing from the northeast,




making the area inaccessible for study by boat; (3) the coastal waters are




shallow near shore, making the area inaccessible to divers; (4) organisms




are not abundant in the sandy intertidal surf zone; (5) the rocky intertidal



zone hosts a diversity of organisms, especially in the tidepools that form




in the rocky outcroppings.



    These conditions indicated that the rocky intertidal zone  should become



the focus for preliminary field observations to determine  the  design of the




major experiments with mosto.  Its value as a study site,  however, was




impaired by movement of sand onto the beach.  By the end of the study




period, sand had covered many of the intertidal communities under




observation.



    Examinations of intertidal samples taken before and after  resumption of




effluent discharge at the outfall site (Table U) and the control site (Table




5) revealed more consistent decreases in abundance of  organisms at the




discharge site than at the control site.  A census of  tidepool  fish revealed



obvious differences in species composition before and  after resumption of




discharge (Table 6).  Certain intertidal organisms, found  to be absent or




rare near the outfall, were suspected of being excluded or limited by the




effluent, and were selected for the field and laboratory bioassay




experiments.  These were the sea urchin Echinometra lucunter,  which was

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                                                                                                     32
Table 4.  Intertldal  samples taken at the discharge site at Arecibo before and after resumption of
mosto discharge
Station 1
Transect 1-B Transect
Before After Before
M VEHTEBH ATES
Limpets
Sea Worms"
Snails
"Beach Hoppers* (shriap)
Clams
Crabs
"Coat-of-Mail"
ALGAE
01 va
Fadlna
Chaetomorpha
Cladophora**
Hypnea
Cracilaria
Caulerpa
imphlroa
Bryocladla
Enteromorpha

10
25
28
11
170
0
0

0
27.2
12.3
Tr
0
Tr
0
0
0
0

0
0
1
3
16
0
0

Tr
0
Tr
Tr
0
Tr
0
0
0
0

4
100 .
4
33
57
2
0

0.6
4.6
29.8
19.6
1.2
1.0
19-3
Tr
Tr
0
2-B
After

0
0
0
0
9
0
0

Tr
Tr
Tr
0
0
0
Tr
0
0
0
Transect 1-W
Before After

0
58
62
52
112
0
8

1.4
0
105.6
23.6
4.8
0
0
0
1.3
0

0
0
0
0
0
0
0

0
0
0
0
0
0
0
0
0
Tr
•Includes nematodes
"•Includes Centroceras and Polysiphonia

ALGAE in grams wet weight    INVERTEBRATES  in  numbers  of Individuals

Source:  Puerto Hioo Report
                                                           Tr = Trace

-------
                                                                                                              33
            Table 5.
                      Intertidal  samples at an upstream control station at Areoibo before and after
                      resumption  of nosto discharge
                                                           STATION 3
Transect 1-B
Before After
IK VERTEBRATES
Urchin
Sea cucumber
Limpet
Segmented worms
Snails
Beach shrimp
•Coat-of-Mail"
Unsegmented worms
Clams
Crabs
ALGAE
Sargassua
Padlna
Laurencla
Cladophora
Dletyopterls
Halynenla
Spyrldla
Enantiocladia
Jania
Plctyota
Anadyonene
Champ i a
Dlgonia
Valonia
Stypopodlum
Bryothamnlon
Heterosiphonia
Caulerpa
Amphiroa
Coelothriz
Cladophoropsls

21
1
2
50
100
133
3
9
0
0

31
Tr
6.8
78
Tr
3.2
Tr
6.6
Tr
0
0
0
0
0
0
0
0
0
0
0
0

11
3
0
16
281
27
0
11
2
0

28.3
Tr
Tr
31.6
0
7.4
0
0
0
Tr
Tr
0
Tr
Tr
0
0
0
0
0
0
0
Transect 2-B
Before After

19
2
0
168
21
267
0
0
0
0

55.4
.1
97
21.9
0
0
0
0
0
0.1
Tr
Tr
0
0
0
0
0
0
0
0
0

10
2
0
9
23
10
0
0
0
0

24.6
0
70.2
0
0
0
0
0
0
Tr
0
0
0
0
0
0
0
0
0
0
Transect
Before

1
1
0
100
93
382
0
0
10
0

21.9
.4
1.4
42.6


3-6

.4
0

184.2

18.7
8
.5
.7
.1

1-W
After

0
0
H
17
910
59
1
0
2
10

Tr
Tr
Tr
Tr


0

0
Tr

170.2

0
0
0
0
0

Transect 2-H
Before After

0
0
1
84
16
150
0
0
0
5

87.5

121.2
0


0

6.7
2.0








0

17

1
34
21
67
1
0
1
1

14.6

111.'
Tr


Tr

0
Tr








Tr
ALGAE In grama wet weight   INVERTEBRATES in numbers  or Individuals   Tr = Trace

Source:  Puerto Rico Report

-------
                                                                                                              3*4
                           TABLE 6.  Tldepool fish species observed on selected dates at Areelbo.

                           Hay 3   Hay 9   May 19   May 23" Hay 29   May 30   June 2»  June  6    June 11   July lt»«»JuIy 5
Opicbthidae
  Chlorhlnua  avenaon

Carangidae
  JOT. Jack                  *

Poaadasyldae
  Juv. grunt                 +

lyphosidae
  Unld. chub

Cbaatodontidae
  Chaetodon atrlatua         +

Ponacentrtdae
  Eupenaoentrua leucoatlctua +
  E. variabilla              +
  abudefduf aaxatllla        +
  A. taurus                  +

Mi«llidae
  JUT. Bullet               +
Labridas
  Thalaaao
             blfaaciatua
  Dold. wrasse

Cllnldaa
  Paraelinus fasclatua

Blemldae
  Dnid. blenny
  Blennius eriatatua

Oobiidae
  Batfaygobius ap.

Aoantburidae
  Aeanthurua ap.
  Acanthurus coerulua

Botbidae
  Bothua lunatua

Ballatidae
  ju». flleflah

Rmber of Species, totals
12
        12
                11
  •fSpeoiea present
  •All  fish observed were dead or exhibiting abnonaal behavior.
 "Mosto discharge starts
•••Moato discharge stops

Source:  Puerto Rico Report

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                                                                     35






absent for about 2.5 km downstream from the outfall point, and the




"coat-of-mail" shellfish Chiton squamosus and mussel Brachiodontes exustus,




both rare in the impacted zone.



    The absence and reduced abundance of the sea urchin Echinometra at




various stations in the plume is one of the effluent's most striking




apparent effects.  Echinometra is believed to play an important role in



determining the structure of the rocky intertidal community by altering




surface areas and thus increasing available habitats for other organisms.




Thus the absence of Echinometra at the study site for a distance of about




2.5 km west of the outfall point could cause a significant change in the




structure and organization of the rocky intertidal community.




    An extensive physical oceanographic study was undertaken  to characterize




the general nearshore processes and water properties at Arecibo.  Near-shore




circulation is controlled by the dominant effects of a strong alongshore




current system toward the west, generated by the prevailing winds.  Only




short-term reversals occur.  The effluent generally  (about 70% of the  time




during daylight hours) is dispersed along the shoreline to the west at a




rate of about 1.8 km/hr (maximum), with northeasterly winds blowing at




average speeds of between  10 to 15 knots.  These observations confirmed




interpretations of aerial photographs of plume behavior.




    Results obtained in the chemical analysis of the receiving waters  at




Arecibo indicate that the impact of the effluent was not  confined to the




immediate area of the outfall.  Changes in BOD,, and COD were  measurable




several kilometers downcurrent from the point of discharge (Tables 7 & 8).




The effluent changes the normal turbidity, temperature, dissolved oxygen and




salinity patterns in the area around the outfall.  Measurements of trace




metals—Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn—in sandy beach sediments at

-------
                                                                     36






Arecibo were low, and no significant differences were apparent between




controls and samples.



    Characterization of the bottom sediment and beach materials indicated




that very little of the organic component of the effluent is held by these




sediments.  Consequently it appears that the effluent remains in the water




column and  is diluted progressively downstream for several kilometers.




Measurements of  turbidity  and inspection of the aerial photographs confirm




this observation.



    Phytoplankton  productivity  in  the coastal waters along the north shore




of Puerto Rico  is  low,  as  expected in tropical waters.  No increase in




productivity was observed  in  the water  column  in  the plume downstream.

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                                                                                                   37
TABLE 7. Biochemical oxygen demand (BODq) at selected distances from rum effluent outfall at PRO, Areelbo
           on various dates in 1978.
                                                           BOD
Location from outfall
                         July 7
No Discharge

 July 12      July 11
                                                                                      Discharge
(relative to normal
current direction)
-2 km
-1 km
Outfall
Ocean entry
.15 km
.3 km
1.5 km
5.0 km
Note: BODc
2
1
1500
180
1
0
1
1
readings near the outfall
1
1
1660
6
1
0
0
0
were high
2
1
1100
5
i»
3
1
1
on all sampling dates.
1
1
1680
770
70
220
6
2
When mosto was
1
1
1590
670
50
13
0
0
not being
2
1
1660
950
100
78
16
6
discharged, an
       unidentified clear liquid, smelling  strongly  of alcohol,  was  flowing  and may well account for the high
       BODg readings.

Source:  Puerto Rico Report.

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                                                                                      38
TABLE 8.   Chemical  oxygen  demand  at  selected  distances frog rum effluent outfall In coastal
          waters at PRO, Areelbo  on  various dates  in 1978.
                                  ODD, mg/1
Location from
outfall
(Relative to normal
current direction)
-2 km
-1 k
Outfall
Ocean entry
.15 km
1.5 km
5 km
July 14

104
97
-
114
110
97
53
July 21

73
53
24000
2000
1400
174
75
70
August 4

122
39
-
164
61
89
August 10

15
92
8448
3456
270
131
34
75
August 24

9
30
11155
99
147
96
134
Dash (-) indicates that measurement was not made.

Source:  Puerto Rico Report

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                                                                     39


BACARDI CORPORATION:  CATANO AND THE ENSENDA DE BOCA VIEJA*

Site Description

    The Bacardi plant (Fig. 9) is situated in the Palo Seco section of

Catano, one of the "municipios" that make up greater San Juan, on the neck

of land that separates the Bay of San Juan on the east from the Ensenada de

Boca Vieja, which receives most of the effluent, on the west.  The Ensenada

is a tidal embayment, or estuary, whose shoreline the Bacardi outfall shares

with the former Schenley plant (now owned by Bacardi), the Palo Seco

Thermoelectric plant, the mouth of the Bayamon River, and Levittown (Fig.

10).

    The grounds of  the distillery do not abut the point of outfall on the

Ensenada de Boca Vieja.  A pipe carries the waste stream from the plant to

the point of discharge (Fig.  11).  The discharge is relatively constant, as

the plant operates  about 300  days a year (except in late December and

January when the plant is shut down for employee holidays and equipment

cleaning and repair).  With production at the main plant at about 60,000

proof gallons a day, and a ratio of about 6 gallons of effluent per proof

gallon of rum, the  effluent load is about 360,000 gallons a day.

    From the outfall pipe, the plume normally fans out in a westerly

direction; its configuration, however, changes  as local current patterns

change.  The water  within the plume is reddish  brown  and opaque.  Dissolved

oxygen measurements indicate  that there is  no dissolved oxygen in the plume

waters for several  kilometers from the point of discharge.  A strong odor of

hydrogen sulfide and the appearance of bubbles  on the surface indicate  that


•Bacardi's NPDES permit specifies outfall  to the Bayamon River and San  Juan
Bay.

-------

Figure 9.  A low altitude oblique aerial  photograph of the Bacardi Plant at
            San Juan.  The well-defined dark brown plume flows west from the
            discharge pipe in the foreground.

-------
                                                                           41
                         ATLANTIC    OCBAN
           ENSENADA  DE
              BOCA  VIEJA
  OLD
SAN JUAN
                                                    * BACARDI
                                                              BAHIA

                                                                DE

                                            C ATA NO  "^SAN  JUAN
Figure  10.  Map of San Juan showing location of Bacardi and the Old San Juan
           Distillery (now owned by Bacardi).

Note:   This map does not reflect recent man-made changes in the course of
       the Bayamon River.
                                  *


Figure 11.  The Bacardi outfall structure extending into the Ensenada de
           Boca Vieja *at  Catano.

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                                                                   42





 bottom sediments are anoxic  during plant discharges.   Fish kills




 were noted  on occasions  of three separate visits  to  this  site.








VIRGIN ISLANDS RUM INDUSTRIES LTD.:   ST.  CROIX AND THE CARIBBEAN SEA11




Site Description



    The VIRIL plant is located on  the Diamond Estate about 2.0 km inland



from the southern and leeward shore of St.  Croix,  V.I. (Fig. 12).




Frederiksted lies about 6.H km to  the west.   The outfall pipe—formerly a




regional sewer duct—discharges the plant's effluent 600 m offshore at a




depth of 6 m.  During the investigations,  the effluent escaped from several




breaks in the pipe as well as from the pipe end {Fig. 13); thus there were




several plumes that coalesced downstream.




    Several  miles to the east of the  outfall lies the Islands' industrial




complex:   Hess Oil Virgin Islands  Corporation, which includes a refinery and




tanker port, and the Martin-Marietta  aluminum processing plant.  The newly



constructed  publicly owned treatment  system (POTW) discharges its wastes to




the southeast of the VIRIL discharge.




    The VIRIL plant discharges about  65,000 gallons of mosto a day with




little additional plant wastewater.   Both BODj. and total solids content




per unit are high compared to PRO  and Bacardi.  VIRIL»s waste stream




contains only small amounts  of fusel  oils and aldehyde heads, since these



are burned in the boilers to generate heat for distillation.

-------
          cFREDERIKSTED
                         ST.   CROIX
                          VIRIL
                          DIAMOND
   SANDY
   POINT
             CARIBBEAN  SEA
                                           I Mile
Figure 12.  Map of St. Croix, V.I., VIRIL site.

-------
Figure 13.  A low altitude aerial photograph of the VIHIL discharge plume along  the southwest coast  of St.
            Croix.  The dark brown plumes flow in a westerly direction with the  prevailing current.
            submarine outfall pipe was broken and leaking as seen by the four distinct  inner plumes.
            five plumes tend to merge downstream.
                                                                                                                           i
                                                                                                                           .

-------
Field Studies




    While information from the studies at the VIRIL plant discharge site




tends to confirm and support the findings of the research at Arecibo and




Palo Seco, results from the Virgin Islands Report can only be considered




observational and inconclusive at the present time.  The research specified




in the protocol was not adequately addressed in the information that has, to




date, been transmitted to EPA, and thus the Agency is treating the Virgin




Islands Report as observational.




    At the VIRIL discharge site, a species census was conducted along three




transects at four substations at 100 m intervals along the plume and one




transect of four substations about 200 m upcurrent (east) from the discharge




point.  Investigators found that the bottom substrate had been denuded of




turtle grass (Thalassia testudinum) in the immediate outfall area, with




growth of turtle grass diminished to a distance of 0.8 km downstream (west)




of the outfall.  The cause of the bare patches, where turtle grass would




normally be expected, cannot definately be ascribed to the rum effluent, or




to any other factors.  Investigators believed, however,  this could be a




function of the mosto-darkened waters  (high turbidity) impeding the passage




of light to the sea  floor and other effects of the effluent.




    The sea urchin Tripneustes esculentes, a different species from the




urchin studied at Arecibo, was selected  for study  in the Virgin Islands  on




the basis of its scarcity on the sea  floor near the outfall  and up to 2  km




downcurrent.  Investigators believed  the  scarcity  of indigenous benthic




organisms, including the Tripneustes,  near the outfall might be associated




with  the denuding of the turtle grass  beds.  Subsequent  bioassays  in the




laboratory showed the effluent to be  directly  toxic to the urchins in all




concentrations tested.

-------
    Divers at St. Croix found that the waste "plume" distributes itself




vertically throughout the water column, from sea floor to surface.  The




plume is clearly visible by air to a point about 6 km west of the outfall.




Upstream from the outfall, the dissolved oxygen readings were generally over




6.0 mg/1 (normal).   Dissolved oxygen was found to be depressed downstream.








BIOASSAY EXPERIMENTS








    In Puerto Rico,  bioassay tests were conducted in the field on two marine




invertebrates, the  urchin Echinometra lucunter at Arecibo and the blue crab




Callinectes sapidus at the Bacardi site.  In the laboratory, bioassays using



mosto in varying concentrations with seawater were conducted on these two,




plus two additional invertebrates, the "coat-of-mail" shellfish Ch j ton




squamosus and the mussel Brachidontes exustus.








Field Bioassays




    A series  of tidepools within 150 m of the outfall point at Arecibo was




designated for intensive  study of the sea urchin Echinometra lucunter (Fig.
    Sea urchins were transplanted to the tidepools from nearby areas on



three occasions, under three different sets of conditions:




    First  transplant:  May  3, before resumption of plant operation  (after a




shutdown period of  nearly a month).  Twenty days after the transplant,



of the urchins survived  (Table 9).

-------
                                                                N
        ATLANTIC  OCEAN      TIDEPOOL 2
                                          TIDEPOOL 1
                           TIDEPOOL 3
                                                EFFLUENT  STREAM
                                                          X LOW TIDE
                                                    SCALE
Figure  1U.  Location of tidepool studies at Arecibo, P.R.
 Source:   Puerto Rico Report

-------
TABLE 9.  Survival of  transplanted Echinoaetra lucunter in tldepools before resumption of effluent discharge at PHD.
Arecibo.
Date
5/3/78
5/1/78
5/19/78
5/23/78"
Cumulative
t of Days
0
1
16
20
Number of Individuals
Tidepool 1 Tldepool 2
100 50
88 50
89* 19
46
Tldepool 3
100
86
90*
84
Tidepool 1
100
79
74
72
Tldepool 5
100
92
69
46
Average Per-
cent in all
Tldepools
-
891
8«(
70J
  •Counts higher than previous ones were due to exceptional water clarity resulting In improved counting efficiency.
 ••Effluent discharge resumed.
Source:  Puerto Rico Report
TABLE 10.  Effects of effluent on dissolved oxygen levels in tldepoola at PRO,  Arecibo.
Estimated*
Event/date Hosto Cone, (t)
Before Discharge
5/9/78
5/19/78
5/23/78
Initial Discharge
5/29/78
6/6/78
Sustained Discharge
without wave exchange
6/12/78
6/20/78
6/27/78
with wave exchange
6/21/78
6/23/78
Discharge Discontinued
7/3/78
7/4/78
7/10/78

0
0
0

0.1
-


0.68
0.12
0.42

0.01
0.25

0
0
0
TIDEPOOL »1
Temp. (°C) 02 (PP">

28.0
29.5
29-0

29.0
31.0


32.0
32.5
29.0

28.0
28.3

33.5
31.5
28.5

11.2
10.8
6.2

4.5
2.5


0.3
1.8
0.4

5.2
5.3

8.5
6.8
6.3
CONTROL TIDEPOOLS
Temp. (°c) 02(ppm)

-
-
-

. _
-


31.5 12.5
32.5 7.0
30.5- 7.2

28.0 6.3
28.2 6.8

— —
28.8 6.0
28.5 5.9
•Estimated by colorlnetry

Source:  Puerto Rico Report

-------
    Second transplant:  After beginning of plant operation and sustained




effluent discharge.  All urchins were dead after 7 days (Fig. 15).




    Third transplant:  During shut-down period between July 3 and July 14.




Forty percent (or more) of the urchins survived after 7 days (Fig. 15).




    Concentrations of mosto could not be exactly determined, but were




estimated, by colorimetry, for Tidepool #1 (Table 10).  In general, survival




increased slightly in tidepools located successively further from the




outfall point.




    Important differences between dissolved oxygen levels in tidepools with




and without mosto were recorded (Table 10).  Generally, the effect of the




effluent was to depress oxygen concentration so that during low tides, when




pools may be stranded, dissolved oxygen levels approached zero.  This




condition was a reversal of normal daytime low tide situations, where




photosynthetic activity by algae will normally supersaturate stranded pools




with dissolved oxygen.  This critical oxygen concentration difference at




least partly contributed to the observed mortalities of tidepool organisms




after effluent discharge resumed.









Laboratory Bioassays




    Laboratory bioassays used pure mosto—that is, distillery slops with no




other waste stream components—in concentrations from 5%  to  .01/6  (or  1:20 to




1:10,000 dilutions in seawater) in an aerated, flow-through assay system.




The four invertebrates used in the bioassays in Puerto Rico responded




differently, as might be predicted, indicating that certain invertebrates




are more sensitive to mosto (per se) than others.  The sea urchin

-------
                                                                50
    100
_J
I
>
cr
z>
CO
UJ
O
cr
UJ
Q.
Effluent stream not  flowing-
Effluent stream flowing
Tide pool   I  •

Tide pool   2  O

Tide pool   3  A
                I       234567

                EXPERIMENT  DURATION  IN  DAYS
   Figure  15.  Survival of transplanted Echinometra lucunter in  tidepools
              at Arecibo with and without effluent discharge.
   Source:  Puerto Rico Report

-------
                                                                     51






Echinometra lucunter and the "coat-of-mail" shellfish Chiton squamosus were




more sensitive than the other test organisms to mosto, as indicated by




mortality in both species (Fig. 16 &  17) and altered behavior in Echinometra




(Fig. 18) at the lowest concentrations tested.  These observations were




supported by field data from Arecibo  where both organisms were absent or




rare in impacted areas.  Concentrations of .01% or more for a distance of




about 1.5 km downcurrent from the outfall were estimated by colorimetry.




    The mussel Brachiodontes exustus  and the blue crab Callinectes sapidus




were more resistant to the rum slops.  These organisms survived in the




laboratory in seawater containing 0.5% mosto (Fig. 19 & 20).  The 96-hour




LC-., the ("lethal concentration" at  which half the organisms die and half




survive through 96 hours) was calculated to be .087$ for the urchin




Echinometra lucunter.  Allowing for an "application factor" of one order of




magnitude (the conventional means of  calculating a dilution level at which




non-persistent pollutants might be safely discharged into receiving waters),




a dilution of 87:1,000,000 or, rounding off, 1:10,000 would be required in




order for this species to avoid being adversely affected by the discharge.




    The mussel Brachidontes was present in the lower zone of the intertidal




rocks in the heavily impacted area at Arecibo (PRD).  Mortalities were




observed, however, during the first weeks of effluent discharge, and the




mussels disappeared from the upper zone of the intertidal rocks. Production




of the byssal threads by which the mussels attach to a substrate was found




altered in a laboratory bioassay (Fig. 21).  The greater number of byssal




threads seen in the lowest concentration tested is probably a reaction that




indicates the mussels have sensed a change in their environment.  It cannot




in any case be seen as an improvement in the condition of the mussels.

-------
                                                                52
       o:
       z>
       CO
       UJ
       o
       tr
       UJ
       Q.
           100
            50
         5 % Mosto

       0.5% Mosto

      0.05% Mosto

           Control

             I
                                              I
I
                        24        48        72        96

                  EXPERIMENT   DURATION  IN  HOURS
Figure 16.  Bioassay of Rum Distillery Waste (mosto) Seawater:  Survival of
           urchin, Echinometra lucunter,  (n =  60 individuals per dilution).
            100
        cr
        z>
        CO
        UJ
        o
        (T
        UJ
        Q.
 Figure 17.
            50
                      5 % Mosto

                    0.5 % Mosto  •

                   0.0 I % Mosto  A

                        Control  A
                                   I
                                           I
      I
                     24     48     72    96    I20    I44    I68

                       EXPERIMENT  DURATION   IN   HOURS
                                                        I92
Bioassay of Rum Distillery Waste (mosto) in Seawater:  Survival
of Chiton squamosus (n =  60 individuals per dilution).
 Source:  Puerto Rico Report  (Fig 16 and 17)

-------
                                                                 53
         DID NOT

          RIGHT

            •i

         120 -
                                   n*38
                                             n*0
                                            5% Mosto  O

                                           0.5 % Mosto  •

                                          0.05 % Mosto  A

                                              Control  A
                                                        35
      to
      o
      z
      o
      o
      111
      to
      UJ
     CD
     Z

     I-
     X
     CD
     or



     a
     2   30' -
                      24         48        72         96


                EXPERIMENT  DURATION  IN  HOURS



Figure 18.  Bioassay of Rum Distillery Waste (Mosto)  in  Seawater:  Effect

           on Righting Behavior  of Echinometra lucunter.
         100

     or
     z>
     CO
     LJ
     o
     cc
     UJ
     Q.
          50
                  5% Mosto O

                0.5 % Mosto •

               0.05 % Mosto A

                    Control A
                                             _L
                                                      J_
                       24        48        72         96


                    EXPERIMENT  DURATION  IN  HOURS



Figure 19.  Bioassay of Rum Distillery Waste (Mosto) in  Seawater:  Survival

           of the Mussel, Brachidontes exustus (n = 60  individuals per

           dilution).

Source:  Puerto Rico Report (Fig.  18  and 19)

-------
                                                                   54
         SOO
     cc
     u
     CO
     UJ
     o
     £E
     UJ
     OL
         50
        5 % Mosto  O
       0.5 % Mosto  •

     0.0 I % Mosto  A
          Control  A
                      I
                     I
      I
I
                     24        48        72        96

                EXPERIMENT  DURATION  IN  HOURS


Figure 20.  Bioassay of Rum Distillery Waste (Mosto)  in Seawater:  Survival
           of the Blue Crab,  Callinectes sapidus  (n  = 30  individuals per
           dilution).
     O
     O
     IT
     £L
         20
      U)
      Ul
      <
      UJ
Figure 21.
                                    it-S7
     5% Moito O
   0^ % Mosto •
  0.05% Mosto A
       Control A
n»58.
        24       48       72        96

   EXPERIMENT DURATION  IN  HOURS


Bioassay of Rum Distillation Waste (Mosto) in Seawater:   Effect
on Byssal Thread Production by the Mussel, Brachidontes  exustus.
Source:  Puerto Rico Report  (Fig. 20 and 21)

-------
                                                                     55






    Callinectes sapidus was highly resistant to mosto in the laboratory, but




did not survive in the field bioassay experiments at Palo Seco (Bacardi).




Secondary effects related to very low dissolved oxygen levels and to the




presence of hydrogen sulfide in the plume at Palo Seco probably caused the




crabs to die in the field.



    In the Virgin Islands, bioassays were conducted using the sea urchin,




Tripneustes esculentus, in mosto concentrations (VIRIL mosto) ranging from




.T\% to .025%.  Dissolved oxygen levels, despite constant aeration at the




mixing tank, dropped severely in test aquaria, and all the urchins were  dead




well before the full 96-hour exposure.  A prolific "growth" of a white slime




was observed over all substrates in the aquaria.  Increased aeration did not




succeed in bringing up the dissolved oxygen  level appreciably, but seemed




only to enhance the growth of the slime, thought to be microbial in nature.




No conclusions could be drawn from these bioassays.








DISCUSSION OF TROPICAL MARINE ECOSYSTEMS AND ORGANIC  EFFLUENTS




    Each of the three  locations under consideration—the north coast of




Puerto Rico, the coastal  embayment near San  Juan,  and the shallow, southern




shore of St. Croix—is unique, as is each marine ecosystem.   There are,




however, commonalities.   The  sites are within  a few hundred kilometers  of




each other and all are in tropical waters.   Thus it is  possible  to make




several generalizations on the differences between  tropical waters and




cooler seas.  Obviously,  the  waters  are warmer than the  northern temperate



seas.  In the warmer  tropical waters there are a greater number  of different




species than  in cooler waters, but  fewer  individuals  of each  species.

-------
                                                                      56






Most of the individual organisms in colder seas are members  of  the  plankton



community that forms the base of the food web.  It is this abundance—the



richness in microscopic plants and animals—that frequently  gives to  cold



oceans their greenish opaqueness that we see in contrast  to  the clear blue



of the tropical sea, where details may be visible to perhaps 10 fathoms  in



depth.



    The tropical marine ecosystem likewise is more stable and diverse than



the ecosyterns of cooler oceans.  The indigenous organisms survive close  to



the upper limit of  their thermal tolerance and are less able to withstand



"stress," or disruption.  The comparative scarcity of planktonic organisms



(particularly phytoplankton) has led some observers to characterize these



waters as "nutrient poor" and subject to improvement by the  addition  of



effluents.  Spokesmen for the industry have contended that since untreated



rum effluents have  been discharged at these sites for many decades, these



discharges may  already constitute an important food source in the ecology  of



Caribbean waters which cannot be removed without significant damage.  A


                                                                         12
further assertion  is  that the receiving waters are "nutrient-deficient".



     If the  receiving  waters  are "nutrient-deficient", this  is so only in



comparison  with cooler waters  in which many features  are  at  variance. The



limiting nutrients, moreover,  that  would  theoretically  enable the tropical



sea to  support  a more abundant  plankton  population  and  hence a richer flora



and fauna and greater biomass,  are  not  carbon compounds,  but inorganic



nutrients such  as  nitrogen,  phosphorus,  and  other trace materials.

-------
                                                                     57






    It is not realistic, then, to anticipate successfully mimicking the




effects of upwelling—the process by which cooler waters rich in inorganic




nutrients rise from the depths of the sea to the surface, where these




nutrients can be put to use—by addition of "rich" organic waste materials




such as mosto to the coastal waters.  The major problem with the addition of



rum effluent to seawater is its high levels of organic carbon compounds and




high BOD (./COD.  The carbon-rich effluents cause a lowering of the




dissolved oxygen level in the receiving waters and,  in some cases, result in



anoxic conditions.  Thus instead of enhancement through the addition of rum




effluent, degradation occurs to an extent that depends upon physical




conditions in the receiving waters.  The heterotrophic microorganisms—




consumers of complex organic substances—that thrive in the waste  stream




deplete the oxygen supply and thus tend to exclude other  indigenous




organisms that normally balance this ecosystem.



    The potential effects of any proposals to dilute rum  wastewater  through




an ocean outfall are at best poorly understood.  Little  is known about the




impact of a long-term discharge of a dilute  organic-rich  effluent  such as




mosto on the tropical marine environment.  Studies  and  field  observations at




existing outfall sites  revealed damage  to marine  organisms.   Based on  the




data presented in the Puerto Rico Report, dilution  could  not  reasonably  be




expected to render mosto discharges beneficial  to  the receiving waters.

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                                                                          58
                                  SECTION  5




            ¥ASTEWATER TREATMENT  AND UTILIZATION  TECHNOLOGIES13
INTRODUCTION



    A study of technologies  available  for  effluent  treatment and utilization




examined many options,  including discharging  the  wastes to public treatment




works, aquaculture,  ocean discharge, land  application,  evaporation, and




biological treatment.   These wastewater  treatment and by-product




recovery/utilization technologies were found  to be generally applicable to




rum effluents.  Numerous  other  technologies were investigated and found to




offer little promise for  treating rum  distillery wastewater.




Summary:




    Discharge to a publicly  owned treatment works (POTW), aquaculture and




ocean discharge:  Discharge  to  a POTW  was  found not to be a feasible option




for any of the distillers.   The effluent was  found unsuitable for




aquaculture systems, primarily  because of the extremely high BOD_.




Further research may yield new  possibilities  in this area.  Although the




Clean Water Act does not  permit ocean  discharge of untreated industrial




wastes, scenarios for ocean discharge  were developed for all three




distillers.




    Land application:  Agricultural utilization and land farming appear to




be practical  disposal options for  at least one distiller, PRO.  Land




reclamation  is  not  a viable disposal option for any of the  three




distillers.   The  combination of potentially available land  and  a dry climate




on St.  Croix suggests  land  farming as a disposal option for VIRIL.  Bacardi,




located in greater  San Juan, is less favorably situated than the other




distillers for  land application.




    Evaporation:  A review  of  available literature indicates that

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                                                                          59


evaporation of mosto to condensed molasses solubles (CMS) is a

technologically feasible treatment option.  The utilization or ultimate

disposal of CMS,  however, may not be economically practical.  Estimates of

Puerto Rican CMS consumption suggest that approximately 18? of the total

theoretical CMS production could be used as an animal feed supplement if

incorporated in the feed at 5% by weight.  If the CMS were incinerated,

potassium salts could be recovered from the ash.  The market for potassium,

however, is uncertain.  CMS also can be used as a raw material from which to

produce an organic nitrogen-phosphorus-potassium (N-P-K) fertilizer.

    Biological treatment:  Rum processing wastewaters can be biologically

treated and the excess sludge can be disposed of adequately.  The most

economical treatment system probably would be anaerobic digestion followed

by an aerobic system, such as an activated sludge process.  The Anamet

system, an anaerobic/aerobic system, is presently under study at PRD, and

appears promising.  The dark color of the effluent would continue to be of

concern in any conventional biological treatment system.

Outline:

    Disposal to a POTW
    Aquaculture
    Ocean Disposal
    Disposal by Land Application
         Agricultural Utilization
         Land Farming
    Evaporation, with CMS By-Product
         Production of CMS
         Utilization of CMS as a Livestock Feed Additive
         Use of CMS as an Intermediate Product
              Incineration
              Potassium recovery from incinerator ash
              Organic fertilizer production from CMS
    Biological Treatment
         Aerobic Treatment of Mosto
         Anaerobic Treatment of Mosto
         Combined Anaerobic/Aerobic Treatment of Mosto

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                                                                          60
DISPOSAL TO A PUBLICLY OWNED TREATMENT WORKS (POTW)




    Disposal to a POTW is frequently considered when specific waste




treatment options are technically or economically infeasible for a




particular industry.  It is then necessary to consider whether such a




facility has the capacity to treat the waste effectively.  Despite a number




of problems associated with high-strength wastes such as mosto,




possibilities for disposal to a POTW were examined for each of the three



distillers under study.








Bacardi;




    The Bacardi Corporation is located within the city limits of Catano,




where there is presently no regional POTW.  Plans have been developed and




approved for a wastewater collection and sewage system (the Bayamon




wastewater system) capable of accommodating population and industrial growth




through the year 2020.  This primary treatment facility will discharge its




effluent through an ocean outfall.  The Bacardi wastewater, if discharged to




the Bayamon wastewater system, would pass through only partially treated.




The BOD,, would be reduced very slightly, if at all, and thus adequate




pollution abatement would not be achieved.








PRO




    Puerto Rico Distillers is located on the western edge of Arecibo, a city




which currently discharges its domestic waste streams directly to the




ocean.  Construction has reportedly begun on a trunk line system to route




wastewaters to a primary waste treatment plant (also under construction)




located on the eastern side of Arecibo.  There are reportedly no present

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                                                                          61






plans to install a trunk line to the western extremities of the city, where




the distillery is located.  Even if collection lines were available, the




mosto discharged from PRD, virtually identical to that of Bacardi, would




pass through the POTW with minimal treatment.








VIRIL



    A small (0.5 million gallon/day) POTW with secondary treatment is




located within several kilometers of the distillery.  Secondary effluent is




discharged to the Caribbean Sea through a 2,7^0 m outfall pipe.




    VIRIL and POTW personnel conducted preliminary discussions concerning




the possibility of discharging mosto to the POTW.  Rum effluent could not be




accepted for treatment because of its high strength.








AQUACULTURE



    Aquaculture, in concept, has several advantages over other utilization




or by-product recovery technologies:  little  or no effluent pretreatment is




required; considerable potential exists for  recycling nutrients from the




waste into a harvestable  crop.



    A great deal of study would be  necessary  to determine how mosto  could




best be used in an aquaculture project and what species  or chain  of  species




would utilize the slops efficiently and constitute a harvestable  crop.




Questions as to design and  location of physical facilities and projected




capital and operating costs likewise would  require  detailed  study.

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                                                                          62






    Two noted authorities in aquaculture  were contacted for information on




the potential for using mosto in crustacean and higher organism aquaculture




systems.    Both agreed that the mosto  material is not adaptable as a feed




ingredient in such aquaculture systems.   Their evaluation was based on




several factors, including the following:




    1. The BODR and COD in mosto are  very high, indicating that the mosto




       is very high in organic carbon compounds;




    2. High carbon content is further substantiated by high carbohydrate




       content;



    3. Presence of significant amounts  of carbon compounds will enhance




       growth of heterotrophic bacteria;




    U. Levels of copper in mosto (average ^330 ppb) are toxic to most




       shellfish and acutely toxic to the larval stages.








OCEAN DISPOSAL




    While the Clean Water Act does not  provide for ocean discharge of




untreated industrial wastes in cases  where a viable treatment technology has




been identified, this option has been explored at the request of the




industry.  As noted in Section 4, possible benefit to the receiving waters




suggested by the rum industry could not realistically be expected.  Thus an




exception to the Clean Water Act on the basis of assumed water quality




enhancement could not be a valid consideration.  We have, nevertheless,




considered various factors related to ocean disposal.




    Effective  use of the ocean  to assimilate wastewater depends  on both



environmental  and engineering factors.   Important environmental  factors are




ocean  depth, currents,  bottom materials and  configuration,  and  density




stratification.  Engineering must allow for good  jet  mixing,  taking  into

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                                                                          63






account these environmental features as well as chemical and physical




characteristics of the specific waste stream.



    A typical outfall consists of a submarine pipeline roughly perpendicular




to the shoreline.  In shallower water, it is buried under rip-rap for wave




protection and at greater depths it is laid on the ocean floor with ballast




rock.  At the end there is usually a diffuser section, a manifold with many




small holes to distribute the flow over a large area of the ocean floor.




Two noted authorities in the design of submarine outfalls were contacted to




assist in evaluating the potential for continued ocean disposal.    It was




estimated that instantaneous dilution of mosto through a properly designed




diffuser would be in the range of approximately 1:50 to 1:100.  The density




of mosto, approximately that of seawater, would aid in dispersion.  Once




discharged, ultimate dispersion is a function of natural oceanic turbulence,




which could be assessed only through procedures beyond the scope of the




study.



    Bacardi has suggested that, rather than  construct its own outfall, it




would connect to the proposed Bayamon primary wastewater treatment plant




(POTW).  The Bayamon outfall would extend 1,215 m  into the Atlantic Ocean to




a depth of about 48 m.  By connecting to the Bayamon outfall, the Bacardi




effluent would mix with the primary treated  effluent from the proposed POTW;




thus, the Bacardi effluent would be "diluted" with sewage, not unpolluted




water.  Such "dilution" might add to pollution problems, not mitigate them.




Additional dilution with seawater could be expected upon discharge to the




ocean through the diffuser.




     In order to  discharge its waste at a depth approaching that proposed by




Bacardi, PRD would have to design and construct an outfall extending 530 m




into the Atlantic Ocean to reach a depth of  approximatley 46 m.  Due to the

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gentle slope of the ocean floor, VIRIL would have to construct an outfall




extending 6,690 m into the Caribbean to reach a depth of *46 m.  Both the PRD




and VIRIL hypothetical outfall designs could be expected to achieve initial




dilutions between 1:50 and 1:100.



    The current practice in outfall design for large systems is to build




long pipelines (3 km)  on the open coast to water depths of 70 m, and to




install large multiple-port diffusers.  These can achieve large initial




dilutions (1:100) and  result in the submergence of the entire wastewater




plume.  It is apparent that none of the ocean outfall scenarios described




above for the distillers meets these criteria for accepted practice for




outfall design and,  thus, may not achieve initial dilutions of 1:100.  This




study did not investigate the biological effects on the marine environment




of discharging mosto through proposed ocean outfalls because such




investigations would have required large expenditures of both time and




resources.   However,  bioassay data from the biological portion of the study




suggested that a dilution of 1:10,000 would be required to avoid adverse




effects on certain marine organisms.








DISPOSAL BY LAND APPLICATION




    Soil, with its tremendous surface area and varied microbial population,




has been viewed as a physical, chemical, and biological filter for waste




materials.  The waste  is retained by the soil matrix, and the organic




fraction of the waste eventually is decomposed by the soil microorganisms.




    Land application is a practice whereby wastes are applied to the soil




surface and incorporated into the top 15 to 30 cm of soil.

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                                                                          65






As in farming practices, only the surface soil is utilized, and the process




of assimilation' is aerobic.  Optimal degradation of the added wastes



requires environmental conditions similar to those needed for raising a



crop—adequate amounts of sunlight, water, nutrients, and oxygen—and




similar types of equipment.



    "Land application" comprises several options.  Principal among these are



the following:




    1. Agricultural Utilization - as a soil amendment, utilizing the



       available organic and inorganic nutrients to enhance the growth of




       certain crops.



    2. Land Reclamation - as a soil amendment to add organic and inorganic



       nutrients to distressed or submarginal lands.



    3. Land Farming - as a means to dispose of wastes on a plot of land, set




       aside for the purpose of disposal, whereby the organic constituents




       are degraded by natural processes  (oxidation and reduction via



       sunlight) and the inorganics are trapped within the surface soil




       matrix.



    Land reclamation is usually employed  in situations where waste



generators are near distressed or heavily mined areas; such lands were not




known to exist within any practical distance of any of the distilleries, and




this option, therefore, was not evaluated.








Agricultural Utilization



    Destilleria Serralles, in Mercedita,  Puerto Rico, has been discharging



its mosto to approximately 160 hectares (ha) of sugar cane fields since  1935




(Fig. 22).

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                                                                          66
Figure 22.   A low altitude aerial photograph of the Serralles Plant in
            Mercedita surrounded by sugar cane fields where the effluent
            from the plant is discharged as fertilizer along with irrigation
            water.

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                                                                          67





    The mosto Is combined with other plant wastes, achieving final dilutions



ranging from 1:2 (other plant wastes/mosto) to 1:5.  This combined stream is



pumped to a central irrigation station which further dilutes the wastes



prior to final field discharge.  The combined plant wastes at this point are



diluted approximatley two-fold.  Supplemental irrigation water is supplied



by an adjacent river north of the plant.  In 1975, a system of peripheral



ditches was constructed to prevent mosto runoff during periods of heavy



rain.  Although soil and groundwater monitoring programs have not been



established, no deleterious effects have been reported for either the soil


             16
or sugarcane.



    Of the three rum distillers considered within this study, PRD, located



within about 7 to 10 kilometers of several thousand hectares of government



owned sugarcane fields, is most favorably situated to take advantage of this



disposal method.



    Neither Bacardi nor VIRIL has similar nearby access to sugarcane or



other crops amenable to irrigation.







Land Farming



    As an alternative to agricultural utilization, the feasibility of



establishing a land farming program for each of the three distillers was



investigated.  Two scenarios were developed:  transportation of mosto



overland via tanker trucks; and, transportation of mosto via pipeline.



    Based on a maximum annual application rate, land requirements for mosto



disposal for Bacardi, PRD, and VIRIL, were estimated at 111 ha, 51 ha,

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                                                                          63






and 24 ha, respectively.  This loading rate was estimated to be equivalent



to 270 dry kg/ha; site life was estimated to be a MniausD of 25 years based



on copper concentrations.  If copper were removed or reduced, the  next



limiting metal, zinc, would extend the site Life to 160 years.








EVAPORATION WITH CMS BY-PRODUCT



    Evaporation of aosto as a treatment technology Bust be  considered as a



function of utilization and disposal options for the resultant products,



CMS car? be used as a livestock feed supplement or as a source of organic



fertilizer,  or it may be incinerated.  In the latter ease,  it has  some value



as a fuel source,  Potassium salts, marketable as a fertilizer additive, can



be recovered from the ash*








Production of CMS



    The technology for evaporation of cane molasaea stillage has been



successfully demonstrated and is eonRsonplace in Europe,  With few



exceptions,  however, it is applied to citrus rather than cane molasses



residues In the U.S.  Typically, CMS has a solids content in excess of 50?,



ft large pilot plant was installed at the Bacardi distillery in Catano in



August 1977, and became fully operational in July 1978.  Currently it is



being evaluated to determine optimum operating conditions for CMS  production,

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                                                                          69






Utilization of CMS as Livestock Feed Additive



    The composition of CMS varies with type of molasses used and, to a




lesser extent, type of fermentation processes and products involved.  Beet




derived CMS has been shown to be a more desirable feed additive than cane




derived CMS because of its higher protein content C\S>% for beet vs. U>6  to 6%




for cane).



    A review of available literature indicated that cane derived CMS could




be safely incorporated into the diets of cattle and laying hens at  the  five




percent level as an additive or as a molasses substitute.  Feed conversion




inefficiencies in cattle and litter caking and staining in laying hens  are




problems that would need to be addressed.



    Additional problems are apparent in the area of marketing.  European  and




U.S. distillers have demonstrated that CMS can be successfully  marketed.




Puerto Rico and the Virgin Islands, however,  have potentially  limited




domestic CMS markets.  Starting  from the annual  quantity  of  formulated  feed




manufactured  in Puerto Rico  (St.  Croix was assumed  to produce  a negligible



amount of feed) and assuming incorporation at the  five percent  level,  it  was




estimated that an annual theoretical maximum  of  18^6 of the  CMS  produced by




all three distillers could be utilized in Puerto Rico. The  actual  maximum




may be significantly lower,  however, due  to  customer  and  manufacturer




reluctance  to  accept a new product.



    Feeding tests, moreover, suggest that  CMS is technically less  adequate



as a feed ration  than  cane molasses  and  that  to  balance this disparity, CMS




market prices  would have  to  be  about 5Q%  lower than those for  cane



          17
molasses.     CMS  costs,  beyond  production, would include  those for

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                                                                          70






movement to dockside, storage,  and carrier loading for shipment to the U.S.



A, possible additional cost would be for transport of the CMS from mainland



ports to livestock feed producers and users.








Use of CMS as an Intermediate Product



    Uses of CMS as an intermediate product for incineration, (which may be



followed by potassium recovery),  and as a raw material for organic




fertilizer were evaluated.



Incineration—



    Incineration of mosto theoretically may be accomplished either directly



in the waste stream or after concentration.  The advantage of concentration



is anticipated cost savings, on both total equipment investment and



operating costs.  Engineers and manufacturers of incinerating equipment have



observed that it would be several times more expensive to incinerate slops



directly than to pre-concentrate and then incinerate.



    Thus, any cost-effective incineration plan is likely to include an



evaporation or concentration step.



    Calculations performed on an analysis for a typical mosto stream



indicated that autogenous combustion (combustion without the need for a



continuous supply of auxiliary  fuel) can take place with a feed total solids



(TS) concentration as low as 35?.  A TS concentration significantly above



35% was determined to be desirable to increase heat recovery in a waste heat



boiler and to decrease the size of the incinerator.



    The literature reviewed contained no descriptions of pilot studies



performed under constant operating conditions.  Both Bacardi and PRD



evaluated incinerator systems,  but these differed  in major features.

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                                                                          71






Thus an economic analysis of each on a comparable basis was not possible.








Potassium Recovery from Incinerator Ash—




    Incinerator ash is basically a crude potash containing potassium salts




and impurities such as calcium and magnesium salts, silica, traces of iron




and manganese, and unburned carbonaceous particles.  Ash has been reported




to contain 37$ potassium (expressed as K_0).  Potassium salts generally




have been reported to make up 65% to 70? of the ash.



    Based on pilot plant studies in India, it was estimated that a




distillery discharging 72,000 gallons of mosto a day could recover 3.17




metric tons of potassium salts (expresed as K?0).




    The Puerto Rican market for potassium was examined.  Based on an



estimated yield of 0.024 kg ash to a liter of mosto (0.2 Ibs./gal), an




annual total of 5,000 t of potassium salts could theoretically be generated



by all three distillers.  Based on average consumption figures of potash and




potassium as direct application materials, annual usage was estimated at




1,500 t.  This is about 30$ of the estimated total quantity of such



materials that could theoretically be produced by the three distillers if




all of their mosto were used in this manner.








Organic Fertilizer Production from CMS—




    The Japanese have developed a process for creating an organic complex




N-P-K fertilizer utilizing CMS as a raw ingredient.  Through a series of



chemical reactions involving CMS, sulfuric and phosphoric acids, and




ammonia, a dried granulated product can be produced.  It was estimated that




the three distillers could produce approximately 5^,000 t annually.

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                                                                          72







BIOLOGICAL TREATMENT



    During the last twenty years,  there has bean extensive r-esearch and




development in biological treatment of wastes from the distillation and




fermentation industries, including wine and beer as well as beet and cane




molasses derivatives.  A thorough  review of the literature was conducted to




evaluate the various biological processes.








Aerobic Treatment of Mosto




    Applications of conventional aerobic systems were reportedly beset with




problems.  Both the trickling filter and activated sludge processes, when




used by themselves with undiluted  mosto, were found unsatisfactory.









Anaerobic Treatment of Mosto




    Anaerobic treatment offers several significant advantages  over aerobic




(especially for high-strength wastes such as mosto) including  the  following:




    1. Energy requirements for digesters are small compared with those of




       their aerobic counterparts.




    2. Sludge generation is 5Q% to 80? less  than  for  aerobic  treatment.




       This markedly reduces costs associated with ultimate sludge handling




       and disposal.




    3. Anaerobic  treatment generates methane gas  which  can be  recovered  and




       used as  an energy source.

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                                                                          73



                                     ifi
    In a 1971* study conducted by EPA,   anaerobic digestion, used as a


sole treatment method for rum distillery wastewaters, was reported to be


partially successful.  Other researchers similarly have experimented with


anaerobic digestion, varying loading rates, mosto dilution, retention times,


and other parameters.  Presently the effluent remaining after anaerobic


treatment of rum distillery mosto would require further treatment.






Combined Anaerobic/Aerobic Treatment of Mosto


    The effluent resulting from anaerobic digestion has been demonstrated to


contain high concentrations of BOD,.  (2,000 to 10,000 mg/1) as well as


immediate oxygen demand and thus is  not suitable for direct discharge to


surface waters.  Further treatment can be effectively accomplished through


conventional aerobic processes, specifically, activated sludge.


    If a treatment system consists of anaerobic digestion followed by an


aerobic process, such as activated sludge treatment; both systems will


produce sludge which must be handled and disposed of ultimately.  If


adequate land is available, sludge may be thickened and dried in lagoons.


If land is in short supply, sludge thickening can be followed by vacuum


filtration and incineration, with ultimate land disposal of the ash.


    Many such combined anaerobic/aerobic systems are in existence.  One such


system, known as Anamet (licensed by AB Sorigona of Sweden), has been shown


to be particularly effective on high strength fermentation, carbohydrate,


and nitrogenous wastes.  This proprietary process differs from other


biological systems in that it has all of the following characteristics:

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    1. Use of a closed tank for the aerobic stage rather than an open pond;

    2. Recovery of methane gas for fuel;

    3, Recycle of digester sludge within the anaerobic process and recycle
       of waste-activated sludge from the aerobic step back to the digester

    4. Optional nitrogen stripping with recovery of ammonium salts.

    5.  Low energy consumption. -


    According to representatives of Sorigona, the results of existing Anamet

plants operating on wastewaters similar to those of rum distilling have been

excellent,  with BODj. reductions of up to 99$ being achieved.  After

visiting the facilities of PRD and laboratory testing of the mosto,

Sorigona's  representatives expressed the belief that mosto could be treated

successfully with Anamet.  An Anamet pilot plant was set up at PRD and began

operating in July of 1978.  Preliminary results are not yet available from

this pilot  study (Figs. 23, 24).

    In addition to the treatment systems discussed, there are a number of

process modifications that can result in considerable pollutant reduction.

These are discussed in Section 3 of this report.

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                                                                           75
Figure 23.  Photograph of the Anamet
    pilot plant in operation at PRD.
    The engineering firm of the AB
    Sorigona of Staffanstorp, Sweden, is
    conducting the test.  Test data were
    not currently available.
 o
 03
 c
 z
 LJ

 •2.
 ^^





 0.
 U.'
 CJ
 03
 O
 cr
 UJ
            H SOLID WASTE
    cr
    HJLU
    1-0
    coo
   [SOLUBLE WASTE
           EXTRACELLULAR
           ENZYMES
                   ACID FORMING
                   BACTERIA
ORGANIC  ACIDS* NH
            METHANE FORMING
            BACTERIA
[METHANE-t-CARBON DIOXIDEJ
           ! [AMMONIA REDUCTION!
               REMAINING
           ORGANIC WASTE+ 02+N
 ACTIVATED
   SLUDGE
         [CLEANED  WASTE WATER
 Figure 2U.  Diagram of Anamet  system.

 Source:  Sorigona  trade  brochure.

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                                                                       76


                                  SECTION 6

                           ECONOMIC ASSESSMENT19



INTRODUCTION

    An economic assessment of the Puerto Rico and U.S. Virgin Islands rum

industries was conducted to describe their economic significance to their

respective economies and to analyze the economic implications of wastewater

treatment.



Summary:

    The rum industry is an important and growing source of government

revenues in 1978 contributing 12.7$ of general fund receipts in Puerto Rico

and 16.4J in the Virgin Islands.   Additional benefits to the economy are

seen in the industry as a source  of employment and in the support industries

related to rum production.

    In order to assess the economic impact of wastewater treatment on the

distilleries under consideration, a system of economic model plants was

developed.  Plants of high or average efficiency were found viable after

relatively large expenditures for wastewater treatment.

Outline:

    Relationship of the Rum Industry to the Economies of P.R. and V.I..
        Federal Excise and Domestic Taxes
        Employment
        Secondary Contribution

    Economic Implications of Wastewater Treatment
        Economic Model Plants
        Effluent Treatment Cost Estimates
        Required and Expected Price Increases
        Impacts on Models' Viabilities
        Other  Impacts

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                                                                       77


RELATIONSHIP OF THE RUM INDUSTRY TO THE ECONOMIES OF PUERTO RICO AND THE

VIRGIN ISLANDS



Federal Excise and Domestic Taxes

    Tax revenues from Puerto Rican bottled and bulk rum are derived from two

basic sources.  First, the U.S. Treasury returns $10.50 excise tax to the

Puerto Rico Department of the Treasury for every proof gallon of rum

produced there and shipped to the mainland.  In 1977, this amounted to

$131.7 million and increased by 36.6$ to $179.9 million in 1978.  Second,

local tax payments, including a $9.50 tax on each proof gallon sold in

Puerto Rico, amounted to $29.5 million in 1977 and $32.7 million in 1978.

    Altogether tax revenues from the rum industry to the Treasury of Puerto

Rico amounted to $212.6 million in FY 1978.  This is 12.7$ of the total

general fund receipts of $1,677.0 million and represents an increase from

11.1$ of general fund receipts in FY 1977.

    This is summarized below for Puerto Rico for the past three years

(million $):


                                      FY 1978       FY  1977      FY 1976
Taxes collected on rum                $  212.6      ~$~16^1.2    $~159.3

General fund revenues                  1,677.0         1,455.9     1,327.1

Revenue from rum as percent
  of general fund                          12.7           11.1         12.0

    A similar situation exists in the Virgin Islands.   Federal excise  taxes

on rum manufactured there are  refunded to  the Virgin Islands' treasury.

These revenues have increased  by 37.1$ from $17.8 million in  1975 to $24.4

million in  1978.  This amounted to  16.4$ of general  fund receipts in  1978.

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                                                                       78
Employment



    Total direct employment in the rum industry in Puerto Rico in 1977 was




1,463 individuals.  This included 769  employees working directly in




production and 694 employees involved  in sales, management and other support




functions.  Bacardi Corporation was the largest employer with 443 employees;




Destilleria Serralles had 433;  and Puerto Rico Distillers employed a total




of 319 individuals.




    The four major bottlers in Puerto  Rico employed a total of 210




individuals.  It is estimated that there are another 400 to 600 people




employed in support industries such as trucking, construction, lawn and




grounds, and special maintenance.



    In the Virgin Islands, the total direct employment in the rum industry




is estimated at 100 individuals.  It is estimated that from 20 to 50 more




are indirectly employed by the industry.  Again this includes various groups




that contract with the two distilleries.




    Altogether, these totals amount to about 0.3? of the total labor force




in both localities.








Secondary Contributions




    The chief secondary contributions of the industry are its support of




Puerto Rican sugarcane and molasses production and bottle and carton




manufacturing.




    Puerto Rico has historically been a net exporter of molasses.  Economic




problems in the sugar cane industry have resulted in the island's becoming  a




molasses importer  in  the  1970s.  This trend will probably continue.  The




Virgin  Islands, also  a historical producer of  sugar, has become  totally




dependent on  imported molasses  for  its  rum production.

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                                                                       79






    About 90% of the bottles used in Puerto Rico by the rum industry are




manufactured on the island by two companies.  Sales to the rum industry




account for a significant portion of the bottle manufacturers' total




business.  Small specialty bottles are generally imported.




    Based upon the quantity of rum bottled in 1978, it is estimated that




from $13 million to $16 million is expended on bottles and cartons.  This




provides a significant source of revenue to the bottle and paperboard




manufacturers.  Further, it is estimated that from 300 to 350 persons are




employed in this segment of the industry.



    Bottles and cartons used by the rum industry in the Virgin Islands are




imported from outside sources, including Puerto Rico.  There is an




increasing trend in both localities, however, to ship bulk concentrated rum




to the U.S. mainland for bottling.








ECONOMIC IMPLICATIONS OF WASTEWATER TREATMENT




    As discussed in Section 3, only five distillers (operating six plants)




presently remain in the U.S. Caribbean islands.  In order to provide a




broader base from which to predict the economic implications of wastewater




treatment, a system of economic model plants was developed.  These models




are representative of the more common financial and economic characteristics




of all existing distilleries.



    A net present value (NPV) analysis was used to determine required price




increases necessary in the case of each of the nine models, under different




conditions, to offset treatment expenditures.  These calculations,




considered with other economic characteristics of the industry, were used to




assess the ability of the industry to raise prices, likelihood of plant




closures, and possible losses of government revenues and employment.

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                                                                       80
Economic Model Plants

    Model plants were developed  in three  sizes  (small,  medium and large),

each with three levels of operational  costs  and profitability, yielding nine

model plants.  Major assumptions are described  below:

(1)  The models were assumed  to  produce  only rum.

(2)  The models operated  250  days a year.

(3)  As required by law,  the  models were  assumed to age the rum at least the
     minimum period of one year.  The  gold rum  was assumed to be aged
     approximately three  years.

(U)  The production processes were assumed to include distillation, aging,
     and bottling.  The bottling operations  could be at the same or at a
     separate facility.

(5)  Since most distilleries  age their rum for  future bottling and,
     accordingly,  must produce in one  year what they expect to sell in a
     future year,  it was  assumed that  the quantity of rum sold in a given
     year would approximate 85%  of that year's  given production.

(6)  The distillery models were  assumed to be technologically modern
     although the actual  facilities, while they have been modernized over
     the years, are fairly old and well depreciated.

(7)  The models contained no  provisions for major expansionary expenditures.

(8)  As most existing distilleries have obtained exemptions from certain
     taxes (which vary by distillery), the models were analyzed using two
     tax scenarios—one with  a tax rate of 25%  and one with the standard 48$
     tax rate.


    Based on the preceding assumptions, the model plants' annual quantities

of rum produced were estimated to be as follows:

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                                                                       81
    Model Size                       Annual Product ion
                                     (proof gallons)

    Small                             2,500,000
    Medium                            7,500,000
    Large                            17,500,000

    Financial profiles of the models were developed to assess their

potential to deal with wastewater treatment expenditures.  For these

profiles, model plant sales, costs, and investment characteristics were

developed from information provided by industry personnel and published

sources.



Effluent Treatment Cost Estimates

    Costs for specific wastewater treatment options were not available for

this analysis.  Thus, incremental treatment costs (capital) were utilized to

describe the point at which each model's impacts would be significant enough

to force closure.  The cost increments varied according to model size, with

the small model's increments being in amounts of $500,000, the medium

model's increments being $1,000,000, and the large model's increments being

$2,000,000.  Annual operating and maintenance expenditures associated with

each increment (capital) were assumed to be 10? of each investment increment

in one scenario and 20% in a second scenario.  For each model's analysis a

total of twenty-five increments were considered.



Required and Expected,Price Increases

    Required price increases differed with model size and  treatment

investment increment.  As expected, the small size models  required the

greatest percentage increase and the large size, the least.  The analysis

projected the increase that would be necessary under two conditions:  that

the plants' partial tax exempt status continue and that  the  tax exemption be

removed.

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                                                                       82






Again, as expected, greater price increases are required with an assumed




loss of tax exemption.



    Economic input analyses indicated that price increases of 2% to &% would




be needed for most increments considered to maintain pre-control (treatment




of rum effluent) levels of profitability.   If distilleries increase their




prices by 2% to 45E, the annual rate of sales increase (currently 9.6$) could




slow measurably for one or two years,  but  total sales would continue to




rise.  After this time, the influences of  income and population trends would




reestablish the market momentum of rum.  If the required price increase were




5% to 8jt of its present price ($5.25 per proof gallon),  the models' rates of




sales growth would slow considerably and,  perhaps, even cease for one or two




years.  After that period, however, the growth rate would begin to increase




again and, within three or four years, the industry would recover its




original rate of growth.



    Considerable variation,  however, could be expected in these projected




price increases because of economies of scale in treatment facilities.




Smaller distilleries will probably pay a higher per unit cost for control




than larger ones.  For smaller distilleries price increases will then be




limited by the new market equilibrium price.  This new market price will




effectively be established by the firm with the largest volume in the market




place.  If this new price is lower than that required to meet the costs




incurred by the smaller distilleries, they will be forced to absorb the




remaining costs in their existing profit margin and/or reduce costs in other




areas, or to cease operations.








Impacts on Models' Viabilities




    The extent to which effluent control costs—beyond those offset by

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                                                                       83


increased prices—would impact production obviously depends upon the

interaction of complex variables.  By applying closure criteria developed

for the study, it was possible to determine the highest treatment investment

increments (capital) and associated operation and maintenance (O&M)

expenditures the models could afford.  These increments depended on the

models' size, operational efficiency level, tax exemption status, and

whether the treatment O&M expenditures were 10% or 20% of the applicable

treatment investment.

    Table 11 depicts the largest investment increment affordable for the

model plants.  As shown, the smallest affordable increments were associated

with the low efficiency models, particularly those analyzed under the

assumption of the standard ^Q% tax rates and the 20% O&M scenario.  Under

these assumptions the low efficiency small model was projected to be able to

afford expenditures of approximately $500,000 or less, the medium model,

$1.0 million or less, and the large model, $2.0 million or less.  For the

average efficiency models, the largest affordable increments were $4.5

million for the small model, $17.0 million for the medium model, and $40.0

million for the large model.  Under less stringent assumptions, some average

efficiency models were projected to be able to afford all the investment
                      *
increments considered.   For the high efficiency models, all investment

increments were projected to be affordable, except the small models which

were projected to be able to afford investment of up to $8.5 million.
•As stated previously, 25 increments of $500,000, $1 million, and $2 million
were considered for the small, medium, and large models respectively.  Thus,
the maximum increment considered was $12.5 million for the small model,
$25.0 million for the medium model, and $50 million for the large model.

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                                                                                                     84
Table 11.  Largest possible capital investment for waste treatment with model plants remaining viable (no closure)

Model                                   Ten$  O&M  Scenario                   Twenty^ O&M Scenario
Type/Size	Investment Increment	Investment Increment

                                          (million dollars)                    (million dollars)

Standard 48< Tax Bate
 Average Efficiency
      .  Small                                     7.0                                  4.5
      .  Medium                                  23.0                                 17.0
      .  Large                                     NC                                 40.0
 High Efficiency
      .  Small                                   11.5                                  8.5
      .  Medium                                    NC                                  NC
      .  Large                                     NC                                  NC
 Low Efficiency
      .  Small                                     1.0                                  0.5
      .  Medium                                    2.0                                  1.0
      .  Large                                     4.0                                  2.0
Modified 25$ Tax Rate
Average Efficiency
. Small
. Medium
. Large
High Efficiency
. Small
. Medium
. Large
Low Efficiency
. Small
. Medium
. Large


7.0
23.0
NC

11.5
NC
NC

2.5
7.0
16.0


5.0
17.0
40.0

8.5
NC
NC

1.5
4.0
8.0
NC:  No closure at any of the  investment  increments considered.
Source:  DPRA Economic Study

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                                                                         85






Other Impacts




    Whether or not government revenues would be impacted by the closure of




one or more of the presently operating rum plants would depend on whether




the market shares thereby lost were absorbed by another distillery.  A




similar situation exists in relation to potential losses in the employment




sector and secondary impacts.

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                                                                       86
                                  REFERENCES

1.  Development Planning & Research Associates,  "Economic Impact of Effluent
    Regulations on the Rum Industry (Puerto Rico and Virgin Islands)," 1979,
    a report prepared for the U.S.  EPA Office of Analysis and Evaluation,
    hereafter cited as DPRA Economic Study.   Unless otherwise noted,
    material in this subsection,  "Industry Structure," is based on
    information contained in this document.

2.  Internal communication with E.'J. Struzeski,  Jr., U.S. EPA National
    Enforcement Investigation Center (NEIC), Denver, CO., October 13, 1978.

3.  The subsection "Process Description" is based on information in personal
    communication from E.J. Struzeski,  Jr.,  U.S. EPA NEIC, Denver, CO.

4.  U.S. EPA Region IV,  Surveillance and Analysis Division (SAD), "Rum
    Industry Wastewater Characterization Study,  Puerto Rico - Virgin
    Islands, July, 1978" (1979).   Unless otherwise noted, the subsection
    "Wastewater Characterization" is based on information contained in this
    document.

5.  Stearns, Conrad & Schmidt Engineers, "Study of Rum Distillery Wastewater
    Treatment and By-Product Recovery Technologies," 1979, a report prepared
    for the U.S.  EPA Office of Research & Development, hereafter cited as
    SCS Technology Study.

6.  SCS Technology Study.  The subsection "Pollution Reduction through
    Process Modifications" is based on Chapter IX of this document.

7.  Erik Krabbe,  U.S. EPA Region  II Facilities Technology Division.
    Testimony of Erik Krabbe for  the adjudicatory hearing in the matter of
    Puerto Rico Distillers, Inc.,  Schenley Industries, Inc., and Virgin
    Island Rum Industries, Ltd.,  1977.

8.  Juan G. Gonzalez ^t al, "Biological Effects of Rum Slops in the Marine
    Environment," 1979,  a report  prepared for the U.S. EPA ORD and the U.S.
    Department of Energy, hereafter cited as Puerto Rico Report.

9.  Michael J. Canoy ^t al, "Environmental Effects and Acceptability of Rum
    Effluents," 1979, a report prepared for the U.S. EPA ORD, hereafter
    cited as Virgin Islands Report.

10. Puerto Rico Report.   Material in Section 4 relating to scientific
    studies in Puerto Rico is based on information contained in this
    document.

11. Virgin Islands Report.  Material in Section 4 relating to scientific
    studies in the Virgin Islands is based on information contained in this
    document.

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                                                                       87
12.  Paul A.  Friedman,  Esq.,  White & Case,  counsel for Seagram Company,
    letter to E.  Zell  Steever,  U.S. EPA ERL-N,  December 29,  1978.

13.  SCS Technology Study.   Unless otherwise noted,  Section 5 is based on
    information contained  in this document.

14.  Dr. Douglas Conklin, University of California,  Bodega Marine Laboratory,
    and Dr.  Michael Hartman, Savannah State College,  Savannah, Georgia,
    letters  to Michael A.  Caponigro,  SCS Engineers, included in the SCS
    Technology Study,  Appendix A.

15.  Dr. Norman H. Brooks and Dr.  R.C.Y. Koh, California Institute of
    Technology, correspondence referenced in the SCS Technology Study
    throughout Chapter VI.

16.  Internal communication with E.J.  Struzeski, Jr.,  U.S. EPA NEIC,
    Denver,  CO.,  October 13, 1978.

17.  DPRA Economic Study.

18.  T.G. Shea £t al, Rum Distillery Slops Treatment by Anaerobic Contact
    Process, a report  prepared for the U.S. EPA ORD,  1974.

19.  DPRA Economic Study.   Section 6 is based on information contained in
    this study.

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