United States
Environmental Protection
Agency
Office of Water
Regulations and Standards
Washington, DC 20460
Water
                June, 1985
Environmental Profiles
and Hazard Indices
for Constituents
of Municipal Sludge:
Dimethyl Nitrosamine

-------
                                PREFACE
     This document is one of a series of p eliminary assessments dealing
with  chemicals  of potential  concern  in municipal  sewage  sludge.    The
purpose of these documents is to:   (a)  summarize  the  available  data  for
the  constituents  of  potential  concern,  (b) identify  the  key  environ-
mental pathways  for  each constituent  related  to a  reuse  and  disposal
option (based on hazard  indices), and  (c) evaluate  the  conditions  under
which such a pollutant  may pose  a hazard.   Each document provides a sci-
entific basis for  making an  initial  determination  of whether a pollu-
tant, at levels currently observed in sludges,  poses a  likely hazard  to
human health  or  the  environment when  sludge  is disposed  of  by any  of
several methods.   These  methods  inclui* > landspreading on food chain  or
nonfood chain crops,  distribution  and marketing  programs,  Landf illing,
incineration and  ocean  disposal.

     These documents  are  intended to serve as a rapid screening tool  to
narrow an initial list  of pollutants  to  those of concern.   If a  signifi-
cant hazard is  indicated  by  this preliminary analysis,  a more  detailed
assessment will  be undertaken  to  better quantify  the   risk  from  this
chemical  and to derive criteria  if warranted.   If a hazard is shown  to
be unlikely, no further assessment will be conducted at  this time; how-
ever, a  reassessment  will be conducted after  initial  regulations are
finalized.  In no case, however, will criteria  be derived solely on the
basis of  information presented in this document.
                     u  „
                     Headquarters and Chemical Libraries
                         EPA West Bldg Room 334Q
                              Mailcode 3404T
                         1301 Constitution Ave NW
                           Washington DC 20004
                               202-566-0556
                          Repository Material
                        Permanent Collection

-------
                            TABLE OP CONTENTS


                                                                      Page

PREFACE  	    i

1.  INTRODUCTION	   1-1

2.  PRELIMINARY CONCLUSIONS FOR DIMETHYL NITROSAMINE
      IN MUNICIPAL SEWAGE SLUDGE	   2-1

    Landspreading and Distribution-and-Marketing 	   2-1

    Landfill ing	   2-2

    Incineration 	   2-2

    Ocean Disposal 	   2-2

3.  PRELIMINARY HAZARD INDICES FOR DIMETHYL NITROSAMINE
      IN MUNICIPAL SEWAGE SLUDGE	   3-1

    Landspreading and Distribution-and-Marketing 	   3-1

         Effect on soil concentration of dimethyl nitrosamine
           (Index 1) 	   3-1
         Effect on soil biota and predators of soil biota
           (Indices 2-3) 	   3-3
         Effect on plants and plant tissue
           concentration (Indices 4-6) 	   3-4
         Effect on herbivorous animals (Indices 7-8) 	   3-6
         Effect on humans (Indices 9-13) 	  3-9

    Landfilling 	  3-15

         Index of groundwater concentration resulting
           from landfilled sludge (Index 1) 	  3-15
         Index of human cancer risk resulting
           from groundwater contamination (Index 2) 	  3-22

    Incineration 	  3-23

    Ocean Disposal  	  3-23

4.  PRELIMINARY DATA PROFILE FOR DIMETHYL NITROSAMINE
      IN MUNICIPAL  SEWAGE SLUDGE	  4-1

    Occurrence 	  4-1
                                   11

-------
                            TABLE OP CONTENTS
                               (Continued)
                                                                     Page
         Sludge 	  4-1
         Soil - Unpolluted 	  4-1
         Water - Unpolluted	  4-2
         Air 	  4-2
         Food 	  4-3

    Human Effects 	  4-4

         Ingestion 	  4-4
         Inhalation	  4-5

    Plant Effect	  4-5

         Phytotoxicity 	  4-5
         Uptake 	  4-5

    Domestic Animal and Wildlife Effects 	  4-6

         Toxicity	  4-6
         Uptake 	  4-6

    Aquatic Life Effects 	  4-6

         Toxicity	  4-6
         Uptake 	  4-7

    Soil Biota Effects 	  4-7

    Physicochemical Data for Estimating Fate and Transport 	  4-7

5.  REFERENCES	  5-1

APPENDIX.  PRELIMINARY HAZARD INDEX CALCULATIONS FOR
    DIMETHYL NITROSAMINE IN MUNICIPAL SEWAGE SLUDGE 	  A-l
                                   111

-------
                                 SECTION 1

                               INTRODUCTION
     This  preliminary  data  profile  is  one  of  a  series  of  profiles
dealing  with  chemical  pollutants  potentially  of  concern  in  municipal
sewage sludges.   Dimethyl nitrosamine  (DMN)  was initially  identified  as
being  of   potential  concern  when   sludge   is  landspread   (including
distribution  and  marketing)  or placed  in  a  landfill.* This profile  is  a
compilation of information  that  may be useful in determining whether DMN
poses an actual hazard  to human  health or the environment when  sludge  is
di-cosed of by these methods.
     The  focus  of  this  document  is  the  calculation  of  "preliminary
hazard indices"  for  selected  potential  exposure  pathways, as  shown  in
Section  3.    Each index  illustrates  the  hazard  that  could result  from
movement  of  a  pollutant by a  given  pathway  to cause  a  given  effect
(e.g., sludge •*• soil •*  plant uptake -» animal  uptake  -*•  human  toxicity).
The  values  and  assumptions  employed  in these  calculations   tend  to
represent a  reasonable "worst  case";  analysis  of  error  or uncertainty
has  been  conducted  to  a  limited degree.   The  resulting value in  most
cases  is  indexed  to unity;  i.e.,  values >1 may  indicate a   potential
hazard, depending upon the assumptions of  the calculation.
     The data used for  index calculation have  been  selected or  estimated
based  on  information  presented  in  the  "preliminary  data   profile",
Section 4.  Information in  the profile is based on  a compilation of the
recent literature.   An attempt  has  been  made to  fill out the profile
outline to the greatest extent  possible.  However, since  this  is a  pre-
liminary analysis, the literature has not been exhaustively perused.
     The "preliminary  conclusions"  drawn  from each  index  in  Section  3
are  summarized  in Section  2.    The preliminary hazard indices  will be
used as a screening  tool  to determine which pollutants and  pathways may
pose a hazard.  Where  a potential hazard  is indicated  by interpretation
of these indices,  further analysis will  include  a  more  detailed exami-
nation of  potential  risks  as  well  as an examination of  site-specific
factors.    These  more  rigorous  evaluations  may  change the  preliminary
conclusions  presented  in Section 2,  which are  based on  a  reasonable
"worst case" analysis.
     The   preliminary   hazard  indices   for  selected  exposure  routes
pertinent    to  landspreading   and    distribution   and   marketing   and
landfilling  practices  are included  in  this  profile.   The calculation
formulae  for  these indices  are  shown in  the Appendix.   The indices  are
rounded to  two significant figures.
  Listings  were  determined  by  a  series  of  expert  workshops  convened
  during  March-May,   1984  by  the  Office  of  Water  Regulations   and
  Standards  (OWRS) to  discuss  landspreading,  landfilling,  incineration,
  and ocean disposal,  respectively, of municipal sewage sludge.
                                  1-1

-------
                                SECTION 2

             PRELIMINARY CONCLUSIONS FOR DIMETHYL NITROSAMINE
                        IN MUNICIPAL SEWAGE SLUDGE
     The  following  preliminary  conclusions  have  been derived  from the
calculation of  "preliminary hazard  indices",  which  represent conserva-
tive or  "worst  case" analyses  of hazard.   The indices  and  their basis
and  interpretation  are  explained  in   Section  3.    Their  calculation
formulae are shown in the Appendix.

  I. LANDSPREADING AND DISTRIBUTION-AND-MARKETING

     A.   Effect on Soil Concentration of Dimethyl Nitrosaoine

          Landspreading  of  sludge  of   high  DMN  concentration  may  be
          expected  to  result   in increased  concentrations  of  DMN  in
          sludge-amended soil (see Index 1).

     B.   Effect on Soil Biota and Predators of Soil Biota

          Conclusions were  not  drawn because  index  values could  not  be
          calculated due to lack of data.

     C.   Effect on Plants and Plant  Tissue Concentration

          Conclusions were  not  drawn because  index  values could  not  be
          calculated due to lack of data.

     D.   Effect on Herbivorous Animals

          The animal  toxicity  due to DMN resulting from  consumption  of
          plants grown  on  sludge-amended  soil could  not be  determined
          due to lack of  data  (see Index 7).  The  inadvertent ingestion
          of sludge-amended  soil  by  grazing animals  is  not expected  to
          pose a toxic hazard due to  DMN (see Index 8).

     E.   Effect on Humans

          Conclusions were  not  drawn  for the indices  of human  cancer
          risk  resulting  from  consumption   of  plants grown  on  sludge-
          amended  soil,  consumption of  animal  products  derived  from
          animals feeding  on  plants grown  on sludge-amended  soil,  or
          consumption of animal  products  derived  from animals  that  have
          inadvertently ingested sludge-amended soil due  to lack  of  data
          (see  Indices  9-11).    Sludge  application  is  not  expected  to
          increase   the   potential  cancer   risk   to   adults  due   to
          inadvertent ingestion  of  sludge-amended  soil  containing  DMN.
          The potential  cancer  risk to  toddlers may  increase  due  to
          inadvertent ingestion  of soil  amended  with sludge  containing
          high  concentrations  of  DMN  (see   Index  12).   The    aggregate
          human  cancer  risk due to  DMN  resulting  from  landspreading  of
          sludge could not  be determined  due to lack of data  (see Index
          13).

                                   2-1

-------
 II. LANDPILLIHG

     Landfilling o   sludge  containing high  concentrations  of DMN  may
     result  in  increc.jed  concentrations  of  DMN in  groundwater at  the
     well  (see  Index  1).    Landfill ing  of   sludge   containing  high
     concentrations of  DMN may result in  increased potential  of  cancer
     risk due to contaminated groundwater in  three  of the eight disposal
     scenarios evaluated (see Index 2).

III. INCINERATION

     Based on  the  recommendations  of  the  experts  at  the OWRS meetings
     (April-May, 1984), an assessment of  this  reuse/disposal  option  is
     not being deducted at  this  time.  The U.S. EPA reserves  the right
     to conduct such an assessment for this  option in the future.

 IV. OCEAN DISPOSAL

     Based on  the  recommendations  of  the  experts  at  the OWRS meetings
     (April-May, 1984), an assessment of  this  reuse/disposal  option  is
     not being conducted at  this  time.  The U.S. EPA reserves  the right
     to conduct such an assessment for this  option in the future.
                                   2-2

-------
                                SECTION 3

           PRELIMINARY HAZARD INDICES FOR DIMETHYL NITROSAMINE
                        IN MUNICIPAL SEWAGE  SLUDGE
I.   LAHDSPREADING AND DISTRIBUTION-AMD-MARKETING

     A.   Effect on Soil Concentration of Dimethyl Nitrosamine

          1.   Index of Soil Concentration (Index 1)

               a.   Explanation -  Calculates concentrations  in  Ug/g  DW
                    of pollutant in sludge-amended  soil.   Calculated for
                    sludges  with  typical  (median,  if  available)  and
                    worst   (95   percentile,   if   available)   pollutant
                    concentrations,  respectively,   for  each   of   four
                    applications.    Loadings (as  dry matter)  are chosen
                    and explained as follows:

                      0 mt/ha  No sludge applied.   Shown  for  all indices
                               for  purposes of  comparison,  to  distin-
                               guish hazard posed  by  sludge  from  pre-
                               existing   hazard   posed   by   background
                               levels  or other sources of the pollutant.

                      5 mt/ha  Sustainable yearly agronomic  application;
                               i.e.,  loading  typical   of   agricultural
                               practice,  supplying   •/'SO  kg   available
                               nitrogen per hectare.

                     SO mt/ha  Higher  single application  as  may  be  used
                               on public  lands,  reclaimed areas  or  home
                               gardens.

                    SOO mt/ha  Cumulative  loading  after  100  years  of
                               application at 5  mt/ha/year.

               b.   Assumptions/Limitations  -   Assumes   pollutant   is
                    incorporated into  the upper  15  cm of  soil  (i.e., the
                    plow  layer),   which has  an  approximate  mass  (dry
                    matter)  of  2  x 10^ mt/ha  and  is  then  dissipated
                    through first order  processes which  can  be expressed
                    as a soil half-life.

               c.   Data Used and Rationale

                      i. Sludge concentration of pollutant (SC)

                         Worst      2.55 pg/g DW

                         The  only  available information  on  DMN  concen-
                         trations   in  sludge is  from a  study by  Brewer
                         et al.   (1980)  in  which   DMN   was   quantified
                                   3-1

-------
           in   6   of  16  sludge  samples  from  a   single
           publicl/-owned  treatment  works  (POTWs).    The
           valu.'  ranged from 0.215  to 0.374 Ug/g WW,  with
           a  mean of  0.272  Ug/g  WW.   Percent  solids  was
           not  specified.  (See  Section  4,  p. 4-1.)

           To  estimate DW concentration,  4 percent  solids
           was  assumed.   If  a concentration of  0  Ug/g  is
           used   for  those   samples  where  DMM  was   not
           quantified, the  resulting mean concentration  is
           2.SS Ug/g DW.

           DMN  was  not detected in a  study of  POTWs in  40
           cit  33  (U.S.   EPA, 1982); therefore, the  Brewer
           et ai..  (1980)  value will  be considered a  worst-
           case  value.   However,  it  cannot  be determined
           from    available    information    whether    the
           detection   limits   of   these  two  studies  were
           comparable.
      ii. Background concentration of pollutant in soil
          (BS) = 0 Ug/g DW

          No  DMN was  detected  in  18  crop  soil  samples
          from  seven  states.    Detection  limit  for  the
          survey was 0.2  ng/g  (West  and Day, 1979).  (See
          Section 4, p. 4-1.)

     iii. Soil half-Life  of pollutant  
-------
B.   Effect on Soil Biota and Predators of Soil Biota

     1.   Index of Soil Biota Toxicity (Index 2)

          a.   Explanation -  Compares  pollutant concentrations  in
               sludge-amended soil with  soil  concentration shown co
               be toxic for some soil organism.

          b.   Assumptions/Limitations -  Assumes pollutant  form in
               sludge-amended  soil   ia   equally  bioavailable  and
               toxic as form used in  study  where toxic effects were
               demonstrated.

          c.   Data Used and Rationale

                 i. Concentration of pollutant in sludge-amended
                    soil (Index 1)

                    See Section 3, p. 3-2.

                ii. Soil concentration  toxic to  soil  biota  (TB)  -
                    Data not  immediately available.

          d.   Index 2  Values  - Values were  not calculated  due  to
               lack of  data.

          e.   Value Interpretation - Value equals factor  by which
               expected soil concentration  exceeds  toxic  concentra-
               tion.  Value >  1  indicates a toxic  hazard  may exist
               for soil biota.

          f.   Preliminary Conclusion -  Conclusion was  not  drawn
               because  index  values  could not be calculated.

     2.   Index of Soil Biota Predator Toxicity (Index  3)

          a.   Explanation  -   Compares   pollutant  concentrations
               expected in tissues  of organisms  inhabiting sludge-
               amended   soil  with  food  concentration  shown  co  be
               toxic to a predator on soil organisms.

          b.   Assumptions/Limitations  -  Assumes  pollutant   form
               bioconcentrated   by  soil  biota   is  equivalent   in
               toxicity to form  used to  demonstrate  toxic  effects
               in  predator.     Effect  level   in  predator  may  be
               estimated from that in a different species.

          c.   Data Used and  Rationale

               i.   Concentration of pollutant  in sludge-amended
                    aoil (Index 1)

                    See Section 3, p.  3-2.
                              3-3

-------
               ii.  Uptake factor of  pollutant  in soil biota (UB) -
                    Data not immediacaly available.

               ill. Peed  concentration  toxic  to  predator  (TR)  -
                    Data not immediately available.

                    Feed concentrations  used to  assess  toxicity to
                    six  species  were not   reported   (Haduagwu  and
                    Bassir, 1980).  (See Section 4, p. 4-9.)

          d.   Index 3  Values - Values  were not  calculated  due to
               lack of data.

          e.   Value Interpretation   Values equals factor by which
               expected  concentration in  soil  biota exceeds  that
               which is  toxic  to predator.   Value >  1  indicates  a
               toxic hazard may exist for predators of soil biota.

          f.   Preliminary  Conclusion -  Conclusion  was   not  drawn
               because index values  could not be calculated.

C.   Effect on Plants and Plant Tissue Concentration

     1.   Index of Phytotoxic Soil Concentration (Index 4)

          a.   Explanation  -  Compares pollutant  concentrations  in
               sludge-amended    soil    with    the    lowest    soil
               concentration shown to be  toxic for some plants.

          b.   Assumptions/Limitations -  Assumes pollutant form in
               sludge-amended  soil    is   equally  bioavailable   and
               toxic as form used in  study where toxic effects  were
               demonstrated.

          c.   Data Used and Rationale

                 i. Concentration of  pollutant  in  sludge-amended
                    soil (Index 1)

                    See Section 3, p.  3-2.

                ii. Soil concentration toxic to plants (TP) -  Data
                    not immediately  available.

          d.   Index 4  Values  - Values were not calculated due to
               lack of  data.

          e.   Value Interpretation - Value  equals factor  by which
               soil concentration exceeds phytotoxic  concentration.
               Value >  1 indicates a phytotoxic  hazard may exist.

          f.   Preliminary  Conclusion  -   Conclusion was  not  drawn
               because  index values  could  not be calculated.
                              3-4

-------
2.   Index of Plant Concentration Caused by Uptake (Index 5)

     a.   Explanation    -     Calculates     expected    tissue
          concentrations,  in  ug/g  DW,  in  plants  grown  in
          sludge-amended soil, using  uptake data for  the most
          responsive   plant    species   in    the    following
          categories:   (1)  plants  included  in the  U.S.  human
          diet; and  (2) plants serving  as animal  feed.  Plants
          used vary according to availability of data.

     bo   Assumptions/Limitations  - Assumes  an uptake  factor
          that is  constant  over  all soil concentrations.   The
          uptake factor  chosen for the human diet  is assumed
          to be representative of  all  crops  (except  fruits) in
          the human  diet.    The  uptake  factor chosen for  the
          animal diet  is  assumed  to  be representative of  all
          crops  in  the  animal diet.    See  also  Index  6  for
          consideration of  phytotoxicity.

     c.   Data Used and Rationale

          i.   Concentration  of  pollutant   in  sludge-amended
               soil (Index  1)

               See Section  3, p.  3-2.

          ii.  Uptake factor of pollutant in plant  tissue (UP)
               - Data not immediately available.

               Dean-Raymond and Alexander (1976)  reported that
               S.06Z of  14C-labelled DMN applied  to  soil  was
               taken  up   and  translocated  to  the   aerial
               portions of  lettuce.   (See Section 4,  p.  4-6.)
               Soil  concentration  of   DMN   was  not  reported;
               therefore,   an  uptake   factor  could  not   be
               derived.

     d.   Index 5  Values - Values  were not calculated due to
          lack of data.

     e.   Value  Interpretation -  Value  equals  the  expected
          concentration in  tissues  of  plants grown  in sludge-
          amended  soil.     However,  any  value  exceeding  the
          value of Index 6  for  the  same or  a similar  plant
          species may be unrealistically high  because  it  would
          be precluded by phytoxicity.

     f.   Preliminary  Conclusion  -  Conclusion was  not  drawn
          because index values could not be  calculated.
                         3-5

-------
     3.   Index  of Plant  Concentration Permitted  b? Phytotoxicity
          (Index 6)

          a.   Explanation  -  The index value  is  the maximum  tissue
               concentration,    in    pg/g    DW,   associated   with
               phytotoxicity  in the  same  or  similar  plant species
               used  in  Index  5.    The   purpose  is  to  determine
               whether  the plant  tissue  concentrations determined
               in  Index S  for  high applications are  realistic,  or
               whether  such  concentrations  would  be precluded  by
               phytotoxicity.   The maximum  concentration  should be
               the highest  at which some  plant  growth still  occurs
               (and  thus   consumption  of  tissi •»   by  animals  is
               possible)  but  above which  consumpc on  by animals is
               unlikely.

          b.   Assumptions/Limitations   -   Assumes   that    tissue
               concentration  will  be  a  consistent  indicator  of
               phytotoxicity.

          c.   Data Used and Rationale

               i.   Maximum  plant  tissue  concentration  associated
                    with  phytoxicity (PP) -  Data   not  immediately
                    available.

          d.   Index 6  Values - Values were not calculated  due  to
               lack of data.

          e.   Value  Interpretation  -  Value  equals  the  maximum
               plant  tissue  concentration  which  is  permitted  by
               phytotoxicity.   Value  is   compared  with  values  for
               the same or  similar plant   species given  by  Index  S.
               The lowest  of  the two indices  indicates  the maximal
               increase  that  can  occur at  any  given  application
               rate.

          f.   Preliminary  Conclusion -  Conclusion was  not  drawn
               because index values could  not be calculated.

D.   Effect on Herbivorous Animals

     1.   Index of Animal Toxicity  Resulting from Plant  Consumption
          (Index 7)

          a.   Explanation  -   Compares   pollutant   concentrations
               expected  in  plant  tissues  grown  in  sludge-amended
               soil with  feed  concentration  shown   to be  toxic  to
               wild or domestic herbivorous  animals.   Does  not con-
               sider  direct  contamination  of  forage  by  adhering
               sludge.
                              3-6

-------
     b.   Assumptions/Limitations  -   Assumes  pollutant  form
          taken up  by  plants  is  equivalent in toxicity to form
          used to demonstrate  toxic  effects in animal.  Uptake
          or  tozicity  in  specific  plants or  animals  may  be
          estimated from other species.

     c.   Data Used and Rationale

            i. Concentration  of  pollutant in  plant  grown  in
               sludge-amended  soil (Index  S)  - Values were not
               calculated due to lack of data.

           ii. Peed concentration  toxic to herbivorous animal
               (TA) = 50 Ug/g  DW

               In prolonged  feeding study, cattle were  fed  a
               diet containing 50  ppm of  DMN (Koppang, 1974).
               After  480  days  of  exposure  and  a  one-year
               depuration   period,   all   animals   exhibited
               occlusion of small  hepatic  veins.   (See Section
               4, p. 4-6.)

     d.   Index 7 Values - Values were not  calculated  due  to
          lack of data.

     e.   Value Interpretation -  Value equals  factor  by which
          expected  plant  tissue  concentration  exceeds  that
          which is  toxic to  animals.   Value > 1  indicates  a
          toxic hazard may exist  for herbivorous animals.

     f.   Preliminary  Conclusion   -  Conclusion was not  drawn
          because index values could not be calculated.

2.   Index of  Animal  Toxicity Resulting from Sludge Ingestion
     (Index 8)

     a.   Explanation -  Calculates the amount  of  pollutant  in
          a  grazing   animal's  diet  resulting   from  sludge
          adhesion  to  forage  or  from  incidental  ingestion  of
          sludge-amended  soil  and  compares  this  with  the
          dietary toxic  threshold  concentration for  a grazing
          animal.

     b.   Assumptions/Limitations  -  Assumes   that  sludge  is
          applied over  and adheres to growing  forage,  or that
          sludge constitutes . 5 percent  of dry matter  in  the
          grazing animal's  diet,  and  that pollutant  form  in
          sludge  is equally  bioavailable  and toxic  as  form
          used to demonstrate  toxic  effects.    Where  no  sludge
          is applied  (i.e.,  0 mt/ha),  assumes  diet is  5  per-
          cent soil  as a basis for comparison.
                         3-7

-------
 Data Used and Rationale

   i. Sludge concentration of pollutant (SC)

      Worst    2.55  jig/g DW

      See  Section  3,  p.  3-1.

  ii. Fraction of  animal diet assumed to be  soil  (GS)
      = 5Z

      Studies  of  sludge  adhesion  to  growing  forage
      following  applications  of liquid or  fil:-*r-cake
      sludge  show  that  when  3  to  6 mt/ha of-  sludge
      solids   is   applied,  clipped  forage  initially
      consists  of  up to  30 percent sludge on a  dry-
      weight  basis (Chaney and  Lloyd,  1979;  Boswell,
      1975).   However,  this  contamination diminishes
      gradually  with time  and growth,  and  generally
      is not  detected in the  following year's growth.
      For  example, where  pastures  amended at  16 and
      32 mt/ha were  grazed throughout  a  growing  sea-
      son  (168 days), average  sludge  content of  for-
      age    was   only    2.14   and    4.75 percent,
      respectively (Bertrand  et  al.,  1981).  It seems
      reasonable to  assume that  animals  may receive
      Long-term  dietary  exposure to 5  percent sludge
      if maintained  on  a forage to  which  sludge is
      regularly  applied.   This estimate of 5 percent
      sludge  is  used regardless of  application  rate,
      since  the above studies  did not  show  a  clear
      relationship between application rate  and  ini-
      tial  contamination, and  since  adhesion is  not
      cumulative yearly because of die-back.

      Studies  of grazing  animals  indicate  that  soil
      ingestion, ordinarily <10  percent  of dry weight
     of diet,  may reach as  high  as  20  percent  for
     cattle and  30  percent  for  sheep during  winter
     months  when  forage  is  reduced  (Thornton  and
     Abrams,  1983).     If   the  soil   were  sludge-
     amended, it  is conceivable that up to 5 percent
     sludge may be  ingested  in this manner  as  well.
     Therefore, this value  accounts   for  either  of
     these scenarios, whether  forage is harvested or
     grazed in the field.

iii. Peed   concentration  toxic  to herbivorous animal
     (TA)  = 50 Ug/g DW

     See  Section 3,  p. 3-7.
               3-8

-------
          d.   Index 8 Values


                                  Sludge Application Rate (mt/ha)
                   Sludge
               Concentration         0       5        SO     500

                  Worst              0     0.0026   0.0026  0.0026
          e.   Value Interpretation  -  Value equals  factor  by which
               expected dietary concentration  exceeds  toxic concen-
               tration.   Value  > 1  indicates a  toxic  hazard  may
               exist for grazing animals.

          f.   Preliminary  Conclusion  -  The  inadvertent  ingestion
               of  sludge-amended   soil  by  grazing  animals  is  not
               expected to pose a toxic hazard due to DMN.

B.   Effect on Humans

     1.   Index   of   Human   Cancer   Risk   Resulting  from   Plant
          Consumption (Index 9)

          a.   Explanation -  Calculates  dietary intake expected to
               result  from  consumption  of  crops  grown on  sludge-
               amended  soil.    Compares  dietary  intake  with  the
               cancer risk-specific intake (RSI) of the pollutant.

          b.   Assumptions/Limitations  - Assumes that  all crops  are
               grown on sludge-amended soil and that all  those con-
               sidered to be  affected  take  up the pollutant at  the
               same rate.   Divides  possible  variations  in  dietary
               intake into two  categories:   toddlers  (18 months to
               3 years) and  individuals over 3 years  old.

          c.   Data Used and Rationale

                 i. Concentration   of  pollutant  in  plant  grown  in
                    sludge-amended soil (Index 5) -  Values were  not
                    calculated due to lack of data.

                ii. Daily human  dietary  intake of  affected  plant
                    tissue (DT)

                    Toddler      74.5  g/day
                    Adult       205   g/day

                    The intake value for adults is  based on  daily
                    intake  of  crop  foods  (excluding  fruit)  by
                    vegetarians (Ryan  et  al.,  1982); vegetarians
                    were  chosen to represent  the worst case.    The
                    value  for toddlers  is based  on the FDA Revised
                    Total    Diet    (Pennington,   1983)   and   food
                              3-9

-------
          groupings  Listed  by the  U.S.  EPA  (1984).   Dry
          weights   for   individual   food    groups   were
          estimated  from composition  data  given by the
          U.S.  Department of  Agriculture  (USDA)  (1975).
          These  values were  composited  to  estimate  dry-
          weight consumption of all non-fruit crops.

    iii.  Average daily  human  dietary  intake of pollutant
          (DI)

          Toddler    0.67 Ug/day
          Adult      2.0  Ug/day

          Based  on  limited  exposure  data,  the  estimated
          average daily  dietary human  intake (DI)  for DMN
          is  less  than  2  ug/day  (U.S.  EPA, 1980).   The
          value  assumes  consumption of 100  g of nitrite-
          preserved  bacon,  plus  exposure due to drinking
          water.   For the  purpose of  the  following  cal-
          culations, the DI  for DMN for  adults is assumed
          to  be  2  ug/day.   The  toddler value  assumes  a
          33Z intake  of  adult  DI estimate.   (See  Section
          4, p. 4-3.)

     iv.  Cancer potency =25.9 (mg/kg/day) ~*

          A   cancer   potency    value    for   DMN    of
          25.9 (mg/kg/day)1  was   derived  by   U.S.   EPA
          (1980) from  a  study  involving  lifetime exposure
          of  rats to a variety of  nitrosamine compounds.
          The  effect  observed  in  this   study  was  liver
          tumors.   Uncertainty factors have  not  been as-
          signed to these data.  (See Section 4,  p. 4-4.)

      v.  Cancer    risk-specific    intake     (RSI)     =
          0.0027 ug/day

          The  RSI   is  the  pollutant  intake  value  which
          results in  an  increase  in cancer  risk  of  10~°
          (1 per  1,000,000).   The RSI  is  calculated  from
          the cancer potency using the  following  formula:

          RSI =  10"6 x 70 kg x 103 ug/mg
                     Cancer potency

d.   Index 9  Values - Values  were not calculated due  to
     lack of data.

e.   Value  Interpretation   -   Value  >   1   indicates   a
     potential increase  in  cancer risk  of  > 10~6 (1  per
     1,000,000).   Comparison with  the  null  index value  at
     0 mt/ha  indicates the  degree to which any  hazard  is
     due  to  sludge   application,  as   opposed   to   pre-
     existing dietary sources.
                   3-10

-------
     f.   Preliminary  Conclusion -  Conclusion  was  not  drawn
          because index /alues could not be calculated.
2.   Index of Human  Cait^pr Risk Resulting  from Consumption of
     animal  Products Derived  from Animals  Feeding  on Plants
     (Index 10)

     a.   Explanation   -  Calculates   human   dietary   intake
          expected to result from  pollutant  uptake by domestic
          animals  given  feed  grown  on  sludge-amended  soil
          (crop or pasture land) but  not directly contaminated
          by adhering  sludge.    Compares expected  intake  with
          RSI.

     b.   Assumptions/Limitations  -  Assumes  that  all  animal
          products are  from animals  receiving  all  their  feed
          from  sludge-amended  soil.   Assumes  that  all  animal
          products  consumed  take   up  the  pollutant  at   the
          highest  rate   observed  for  muscle of  any  commonly
          consumed species  or at  the  rate  observed for  beef
          liver  or  dairy  products   (whichever   is  higher).
          Divides  possible  variations  in  dietary  intake  into
          two categories:  toddlers (18  months  to 3 years) and
          individuals over 3 years  old.

     c.   Data  Used and Rationale

           i.  Concentration of  pollutant  in  plant  grown  in
               sludge-amended soil   (Index 5) -  Values were not
               calculated due to  lack of data.

          ii.  Uptake  factor  of   pollutant  in  animal   tissue
               (UA) - Data not  immediately available.

         iii.  Daily human  dietary intake  of  affected  animal
               tissue (DA)

               Toddler    43.7  g/day
               Adult       88.5  g/day

               The fat intake values  presented, which comprise
               meat,  fish,  poultry,  eggs  and  milk  products,
               are derived  from the  FDA  Revised  Total  Diet
               (Pennington,   1983),  food  groupings  listed  by
               the U.S.  EPA  (1984) and  food composition  data
               given  by USDA (1975).  Adult intake  of meats  is
               based  on  males  25 to 30  years of  age and  that
               for milk  products  on  males  14  to  16 years  of
               age, the  age-sex groups  with the  highest daily
               intake.    Toddler intake of  milk  products  is
               actually   based  on  infants,  since  infant  milk
               consumption is the highest among that age group
               (Pennington,  1983).
                        3-11

-------
          iv.  Average  daily human dietary intake of  pollutant
               (DI)

               Toddler    0.67 ug/day
               Adult      2.0 yg/day

               See Section 3, p. 3-10.

           v.  Cancer risk-specific intake (RSI) =
               0.0027 yg/day

               See Section 3, p. 3-10.

     d.   Index  10 Values  - Values were  not  calculated due to
          lack of data.

     e.   Value Interpretation - Same as for Index 9.

     f.   Preliminary  Conclusion  -  Conclusion  was  not' drawn
          because index values could not be calculated.

3.   Index of Human  Cancer Risk Resulting from Consumption of
     Animal  Products   Derived   from   Animals  Ingesting  Soil
     (Index 11)

     a.   Explanation   -   Calculates   human    dietary   intake
          expected  to  result   from   consumption   of  animal
          products derived   from  grazing animals  incidentally
          ingesting  sludge-amended soil.    Compares  expected
          intake with RSI.

     b.   Assumptions/Limitations  -   Assumes  that  all  animal
          products  are  from  animals  grazing  sludge-amended
          soil,  and that all animal  products consumed  take up
          the  pollutant  at  the  highest  rate  observed  for
          muscle  of  any commonly  consumed  species  or at  the
          rate  observed  for  beef  liver or  dairy  products
          (whichever  is higher).   Divides possible  variations
          in  dietary  intake  into two categories:    toddlers
          (18 months  to 3 years)  and  individuals over  3  years
          old.

     c.   Data Used and Rationale

            i. Animal  tissue - Data not immediately available.

           ii. Sludge  concentration of pollutant (SC)

              Worst    2.55  Ug/g  DW

              See Section 3, p.  3-1.
                        3-12

-------
      iii. Background  concentration of  pollutant  in  soil
          (BS) = 0 Ug/g  DW

          See Section 3, p. 3-2.

      iv. Fraction of animal  diet assumed to be soil  (GS)
          = 5Z

          See Section 3, p. 3-8.

       v. Uptake  factor  of  pollutant   in  animal  tissue
          (UA) - Data not immediately available.

      vi. Daily  human dieta  7  intake of affected animal
          tissue (DA)

          Toddler    39.4 g/day
          Adult      82.4 g/day

          The affected  tissue intake  value is  assumed to
          be  from  the fat  component  of meat  only (beef,
          pork,    lamb,    veal)    and    milk    products
          (Pennington,  1983).   This  is  a  slightly  more
          limited choice than for Index  10.   Adult intake
          of  meats  is based  on males 25  to  30 years of
          age and  the intake for milk  products on males
          14  to  16  years of age,  the age-sex  groups with
          the highest  daily  intake.    Toddler  intake of
          milk  products  is  actually based  on  infants,
          since  infant   milk  consumption is  the  highest
          among that age group (Pennington,  1983).

     vii. Average daily  human dietary intake  of pollutant
          (DI)

          Toddler    0.67 Ug/day
          Adult      2.0 Ug/day

          See Section 3, p.  3-10.

    viii. Cancer risk-specific intake  (RSI)  =
          0.0027 Ug/day

          See Section 3, p.  3-10.

d.   Index 11 Values  -  Values were not  calculated  due to
     lack of data.

e.   Value Interpretation -  Same as for  Index  9.

f.   Preliminary Conclusion  -  Conclusion was  not  drawn
     because index  values could  not be calculated.
                   3-13

-------
4.   Index of Human Cancer Risk from Soil Ingestion (Index 12)

     a.   Explanation -  Calculates  the amount  of  pollutant  in
          the diet  of a  child who  ingests soil  (pica  child)
          amended with sludge.  Compares this amount with RSI.

     b.   Assumptions/Limitations  -  Assumes   that   the  pica
          child  consumes an  average  of  5 g/day  of  sludge-
          amended soil.   If  the  RSI specific  for a  child  is
          not available,  this index assumes the  RSI  for a  10
          kg child is the same  as  that  for a 70 kg  adult.   It
          is  thus  assumed  that  uncertainty factors  used  in
          deriving the  RSI  provide  protection  for  the  child,
          taking into  account  the  smaller  body  size and any
          other differences  in sensitivity.

     c.   Data Used  and Rationale

            i. Concentration  of  pollutant   in   sludge-amended
               soil  (Index 1)

               See Section 3,  p. 3-2.

           ii. Assumed amount  of soil  in human diet  (DS)

               Pica  child   5   g/day
              A'dult         0.02 g/day

              The  value  of  5  g/day for  a pica  child  is  a
              worst-case  estimate   employed  by  U.S.  EPA's
              Exposure  Assessment  Group  (U.S. EPA,  1983a).
              The  value  of  0.02  g/day  for an  adult  is  an
              estimate from U.S. EPA, 198A.

          iii. Average  daily human dietary intake of pollutant
              (DI)

              Toddler    0.67 ug/day
              Adult      2.0  Ug/day

              See Section 3,  p. 3-10.

           iv. Cancer risk-specific intake (RSI) =
              0.0027 Ug/day

              See Section 3, p. 3-10.
                        3-14

-------
               d.   Index  12 Values
                                                   Sludge Application
                                                      Rate  (me/ha)
Group
Toddler
Adult
Sludge
Concentration
Worst
Worst
0
250
740
5
260
740
50
363
740
500
260
740
               e.   Value Interpretation - Same as for 4. idex 9.

               f.   Preliminary  Conclusion -  Sludge application  is not
                    expected  to  increase  the  potential  cancer  risk to
                    adults  due  to   inadvertent   ingestion  of  sludge-
                    amended  soil  containing  DMN.    The  potential cancer
                    risk  to  toddlers  may  increase  due to  inadvertent
                    ingestion  of  soil  amended with sludge  containing
                    high concentrations of DMN.

          5.   Index of Aggregate Human Cancer Risk  (Index 13)

               a.   Explanation  -   Calculates  the   aggregate  amount  of
                    pollutant in the  human diet resulting  from pathways
                    described in Indices  9 to 12.   Compares  this amount
                    with RSI.

               b.   Assumptions/Limitations - As described  for  Indices  9
                    to 12.

               c.   Data Used and Rationale - As described  for  Indices  9
                    to 12.

               d.   Index 13 Values -  Values  were not calculated  due to
                    lack of data.

               e.   Value Interpretation - Same as  for Index 9.

               f.   Preliminary  Conclusion -  Conclusion was  not  drawn
                    because index values could not  be calculated.

II.  LANDPILLING

     A.   Index  of  Groundwater Concentration  Resulting  from Landfilled
          Sludge (Index 1)

          1.   Explanation -  Calculates  groundwater  contamination  which
               could occur  in  a  potable aquifer in  the  landfill  vicin-
               ity.    Uses  U.S.  EPA's  Exposure Assessment  Group  (EAG)
               model,  "Rapid Assessment of Potential  Groundwater  Contam-
                                  3-15

-------
     inacion  Under Emergency  Response Conditions"  (U.S.  EPA,
     1983b).  Treats  landfill  leachate as a pulse input,  i.e.,
     the application  of a constant  source  concentration for a
     short time  period  relative  to the time frame of the anal-
     ysis.   In  order to  predict  pollutant movement  in  soils
     and groundwater, parameters regarding  transport and  fate,
     and boundary  or  source conditions are  evaluated.   Trans-
     port  parameters   include  the  interstitial  pore  water
     velocity  and  dispersion   coefficient.    Pollutant   fate
     parameters  include the degradation/decay  coefficient and
     retardation factor.   Retardation is primarily  a function
     of  the  adsorption  process, which  is  characterized  by  a
     linear,  equilibrium  partition  coefficient  representing
     the ratio  of adsorbed  and  solution pollutant  concentra-
     tions.   This  partition coefficient, along with soil bulk
     density and volumetric  water content,  are used to calcu-
     late  the  retardation  factor.    A  computer program  (in
     FORTRAN) was  developed to  facilitate  computation  of the
     analytical solution.  The program predicts pollutant con-
     centration as a  function  of time  and location in both the
     unsaturated and  saturated  zone.     Separate  computations
     and parameter estimates are required for each  zone.   The
     prediction  requires  evaluations   of  four  dimensionless
     input  values  and  subsequent  evaluation  of the  result,
     through use of the computer program.

2.   Assumptions/Limitations - Conservatively assumes  that the
     pollutant  is  100  percent mobilized in  the   Leachate  and
     that all  leachate  leaks out  of the  landfill in a  finite
     period and  undiluted  by precipitation.  Assumes  that all
     soil and aquifer properties are homogeneous  and isotropic
     throughout each zone; steady,  uniform  flow occurs  only in
     the vertical  direction throughout  the unsaturated  zone,
     and only  in  the  horizontal  (longitudinal)  plane   in  the
     saturated zone;  pollutant movement  is  considered only in
     direction of groundwater  flow  for the  saturated zone;  all
     pollutants exist  in concentrations  that  do   not  signifi-
     cantly affect water  movement; for organic chemicals,  the
     background concentration  in the  soil  profile  or  aquifer
     prior to release  from the  source is assumed to be  zero;
     the pollutant  source is a pulse input; no  dilution  of che
     plume  occurs  by  recharge  from outside  the   source  area;
     the leachate  is  undiluted  by aquifer  flow  within  the
     saturated zone;  concentration  in  the   saturated  zone  is
     attenuated only by  dispersion.
                        3-16

-------
3.   Data Used and Rationale

     a.   Unsaturated zone

          i.   Soil type and characteristics

               (a)  Soil type

                    Typical    Sandy loam
                    Worst      Sandy

                    These  two  soil  types were  used by  Gerritse et
                    al.  (1982)  to  measure partitioning  of elf 
-------
          Values,  obtained  from  R.  Griffin  (1984)  are
          representative values for subsurface soils.

ii.  Sit4; parameters

     (a)  Landfill leaching time (LT) = 5 years

          Sikora  et  al.  (1982)  monitored  several  sludge
          entrenchment sites throughout  the United  States
          and estimated time of  landfill  leaching to be 4
          or 5 years.  Other types  of  landfills  may leach
          for longer periods of  time; however, the  use of
          a value  for  entrenchment sites  is  conservative
          because  it   results  in   a  higher   leachate
          generation rate.

     (b)  Leachate generation rate  (Q)

          Typical    0.8 in/year
          Worst       1.6 m/year

          It  is   conservatively   assumed   that   sludge
          leachate enters  the  unsaturated  zone  undiluted
          by precipitation  or  other  recharge,  that  the
          total  volume  of liquid  in the  sludge leaches
          out  of the landfill,  and that leaching is  com-
          plete  in 5 years.  Landfilled  sludge is assumed
          to be  20 percent solids by volume, and depth of
          sludge in  the landfill  is  5 m  in  the typical
          case  and  10 m  in  the worst  case.    Thus,  the
          initial   depth  of  liquid  is  4  and  8 m,   and
          average  yearly  leachate  generation  is  0.8  and
         •1.6 m,  respectively.

     (c)  Depth  to groundwater  (h)

          Typical     5  m
          Worst       0  m

          Eight  landfills  were monitored  throughout  the
          United  States and  depths to  groundwater  below
          them were listed.   A typical  depth  to ground-
          water  of  5 m was  observed  (U.S. EPA,   1977).
          For  the worst case,  a  value of 0 m  is  used to
          represent the situation where  the bottom of  the
          landfill  is  occasionally  or  regularly below  the
          water  table.   The depth  to  groundwater  must be
          estimated  in order  to evaluate  the  likelihood
          that pollutants  moving through  the  unsaturated
          soil will reach the groundwater.
                   3-18

-------
     (d)  Dispersivity coefficient (a)

          Typical    O.S m
          Worse      Not applicable

          The  dispersion process  is exceedingly complex
          and  difficult  to  quantify,  especially  for the
          unsaturated zone.   It  is  sometimes  ignored in
          the  unsaturated  zone, with  the  reasoning that
          pore water  velocities are usually  large enough
          so   that  pollutant   transport   by  convection,
          i.e., water movement,  is paramount.   As a rule
          of  thumb,  dispersivity  may  be  set  equal  to
          10 percent  of  the distance  measurement  of the
          analysis  (Gelhar  and  Axness,   1981).    Thus,
          based on depth to  groundwater  Listed above, the
          value for the  typical  case is 0.5  and that for
          the  worst  case  does  not  apply  since leachate
          moves directly to the unsaturated zone.

iii. Chemical-specific parameters

     (a)  Sludge concentration of pollutant (SC)

          Worst    2.55  mg/kg DW

          See Section  3,  p.  3-1.

     (b)  Soil half-life of pollutant (4) = SO  days

          See Section  3,  p.  3-2.

     (c)  Degradation  rate  (u) = 0.014 day'1

          The unsaturated zone  can serve  as  an  effective
          medium  for   reducing  pollutant   concentration
          through  a  variety  of  chemical  and  biological
          decay mechanisms  which  transform  or  attenuate
          the pollutant.   While these decay processes  are
          usually  complex,  they  are  approximated  here by
          a   first-order  rate constant.    The  degradation
          rate is  calculated  using  the following  formula:
      (d)  Organic  carbon  partition coefficient  (Koc) =
          0.04  mL/g

          The   organic  carbon  partition  coefficient   is
          multiplied   by   the   percent   organic  carbon
          content  of  soil  (foc)  to  derive a  partition
          coefficient  (Kj),  which  represents the ratio  of
          absorbed    pollutant    concentration    to   the
                   3-19

-------
               dissolved  (or  solution)  concentration.    The
               equation  d:oc  x   foc)   assumes  that  organic
               carbon  L.  the  soil  is  the  primary  means  of
               adsorbing organic  compounds  onto  soils.   This
               concept serves  to  reduce much of  the  variation
               in  Kj values  for  different soil  types.    The
               value of Koc is from Hassett et al. (1983).

b.   Saturated zone

     i.   Soil type and characteristics

          (a)  Soil t;pe

               Typical    Silty sand
               Worst      Sand

               A silty sand having the values of  aquifer por-
               osity and hydraulic conductivity defined  below
               represents  a typical  aquifer material.  A more
               conductive  medium  such as  sand  transports  the
               plume more  readily and with  less  dispersion and
               therefore represents a reasonable worst case.

          (b)  Aquifer porosity (0)

               Typical   0.44  (unitless)
               Worst     0.389 (unitless)

               Porosity is  that portion of  the  total  volume of
               soil that  is made  up  of voids (air) and water.
               Values corresponding  to  the above  soil  types
               are  from Pettyjohn et al.  (1982)  as  presented
               in U.S. EPA  (1983b).

          (c)  Hydraulic conductivity of the aquifer (K)

               Typical   0.86  in/day
               Worst      4.04  m/day

               The  hydraulic  conductivity (or permeability)  of
               the  aquifer  is  needed to estimate flow velocity
               based  on Darcy's Equation.   It  is a measure  of
               the  volume  of  liquid  that  can  flow  through  a
               unit area  or media  with  time; values  can  range
               over nine  orders of  magnitude depending on  the
               nature of the  media.   Heterogenous conditions
               produce large  spatial  variation  in   hydraulic
               conductivity,  making   estimation  of   a  single
               effective  value  extremely  difficult.    Values
               used  are  from  Freeze   and   Cherry  (1979)   as
               presented in U.S. EPA  (1983b).
                        3-20

-------
     (d)  Fraction of organic carbon (fOc) =
          0.0 (unitless)

          Organic  carbon  content,  and therefore  adsorp-
          tion, is assumed to be 0 in the saturated zone.

ii.  Site parameters

     (a)  Average hydraulic gradient between  Landfill and
          well (i)

          Typical    0.001 (unitless)
          Worst      0.02   (unitless)

          The  hydraulic  gradient   is  the  slope  of  the
          water  table  in  an  unconfined  aquifer,  or  the
          piezometric  surface  for  a   confined  aquifer.
          The  hydraulic   gradient  must   be  known   to
          determine  the   -magnitude   and  direction   of
          groundwater flow.   As gradient  increases,  dis-
          persion  is reduced.    Estimates of typical  and
          high gradient values  were provided by  Donigian
          (1985).

     (b)  Distance from well to  landfill  (A&)

          Typical    100 m
          Worst       SO m

          This   distance   is   the   distance   between   a
          landfill and  any functioning public or  private
          water supply  or  livestock water  supply.
     (c)  Dispersivity coefficient (a)

          Typical    10 m
          Worst       5 m

          These  values  are  10 percent  of  the  distance
          from well  to landfill  (AJJ,  which  is  100  and
          50 m,  respectively,  for   typical  and   worse
          conditions.

     (d)  Minimum thickness of saturated zone (B)  = 2 m

          The  minimum aquifer  thickness  represents  the
          assumed  thickness  due  to   preexisting   flow;
          i.e., in the absence of leachate.  It is  termed
          the  minimum  thickness  because  in  the  vicinity
          of  the  site  it  may  be increased  by  leachate
          infiltration from  the  site.    A value  of  2  m
          represents   a   worst   case   assumption   that
          preexisting flow is  very  limited and therefore
                   3-21

-------
                    dilution  of  the  plume  entering  the  saturated
                    zone is negligible.

               (e)  Width of landfill (W) = 112.8 m

                    The  landfill  is  arbitrarily  assumed   to  be
                    circular with an  area of 10,000 m2.

          iii. Chemical-specific parameters

               (a)  Degradation rate  (u) = 0 day"1

                    Degradation  is   assumed  not  to  occur  in  the
                    saturated zone.

               (b)  Background   concentration   of   pollutant   in
                    groundwater (BC)  = 0  ug/L

                    It is  assumed  that  no  pollutant  exists  in the
                    soil  profile  or  aquifer prior  to release  from
                    the source.

     4.   Index Values - See Table 3-1.

     5.   Value Interpretation -  Value  equals the maximum  expected
          groundwater concentration  of  pollutant, in  Ug/L,  at  the
          well.

     6.   Preliminary Conclusion - Landfilling of  sludge  containing
          high  concentrations  of  DMN  may  result   in   increased
          concentrations  of DMN in groundwater at the  well.

B.   Index  of   Human  Cancer   Risk Resulting  from  Groundwater
     Contamination (Index 2)

     1.   Explanation  -  Calculates   human  exposure  which   could
          result from groundwater contamination.  Compares  exposure
          with cancer risk-specific intake  (RSI)  of  pollutant.

     2.   Assumptions/Limitations -  Assumes long-term  exposure  to
          maximum concentration at  well at  a rate of 2 L/day.

     3.   Data Used and Rationale

          a.    Index  of  groundwater  concentration  resulting  from
               landfilled sludge (Index  1)

               See Section  3, p.  3-24.
                             3-22

-------
               b.   Average human  consumption of  drinking  water  (AC)  =
                    2 L/day

                    The value  of 2  L/day is  a standard  value used  by
                    U.S.  EPA in most  risk assessment studies.

               c.   Average daily human dietary intake  of  pollutant (DI)
                    = 2.0 ug/day

                    See Section 3,  p.  3-10.

               d.   Cancer risk-specific intake (RSI) =
                    0.00?? Mg/day

                    See Section 3,  p.  3-10.

          4.   Index 2 Values - See Table 3-1.

          5.   Value  Interpretation  -  Value  >1   indicates  a  potential
               increase in  cancer  risk of  10~6 (1  in  1,000,000).   The
               null index value should be used as  a  basis  for comparison
               to  indicate  the  degree  to   which   any  risk  is  due  to
               landfill  disposal,  as   opposed  to  preexisting  dietary
               sources.

          6.   Preliminary Conclusion - LandfiLling  of  sludge containing
               high  concentrations  of  DMN  may   result   in  increased
               potential  of cancer risk due to contaminated  groundwater
               in three of the eight  disposal scenarios  evaluated.

III. INCINERATION

     Based on  the  recommendations  of  the experts  at  the  OWRS  meetings
     (April-May,  1984),  an  assessment  fo this  reuse/disposal option  is
     not  being conducted  at this time.   The U.S. EPA reserves  the  right
     to conduct such an assessment  for  this  option  in the future.

IV.  OCEAN DISPOSAL

     Based on  the  recommendations  of  the experts  at  the  OWRS  meetings
     (April-May,  1984),  an  assessment  fo this  reuse/disposal option  is
     not  being conducted  at this time.   The U.S. EPA reserves  the  right
     to conduct such an assessment  for  this  option  in the future.
                                  3-23

-------
                TABLE 3-1.  INDEX OF GROUNDWATER CONCENTRATION RESULTING  FROM  LANDFILLED  SLUDGE  (INDEX 1)  AND
                            INDEX OF HUMAN CANCER RISK RESULTING FROM  GROUNDWATER  CONTAMINATION
                            (INDEX 2)
V
ro
Sice Characteristics
Sludge concentration
Unsaturated Zone
Soil type and charac-
teristics'*
Site parameters6
Saturated Zone
Soil type and charac-
teristics^
Site parameters^
Index 1 Value (pg/L)
Index 2 Value
1
T

T

T

T

T
9.0x10"*
740
2
U

T

T

T

T
9.0xl»c
345
T

W

T

T

T
2.8x10-3
740
T

NA

W

T

T
6.9xlO-2
790
T

T

T

W

T
4.8xlO-3
740
6
T

T

T

T

W
3.6xlO-2
770
7 8
W N

NA N

W N

W N

W N
14.8 0
12000 740
      aT - Typical values used; W = worst-case values used; N - null condition, where no landfill exists, used as
       basis  for comparison; NA = not applicable for this condition.
      D Index values  for combinations other than those shown may be calculated using the formulae in the Appendix.
      c See Table A-l  in Appendix for parameter values used.
      d Dry bulk density (P
-------
                              SECTION t>

          PRELIMINARY DATA PROFILE PO^ DIMETHYL NITROSAMINE
                      IN MUNICIPAL SEWAGc.  SLUDGE
I. OCCURRENCE

   The principal sources of preformed nitrosamines    NAS, 1978
   appear to be diet and tobacco smoke, but urban     (p. 443)
   and industrial air may prove to contribute much
   to the exposure to preformed nitrosamines.
   Insufficient data exist to quant'fy the
   importance of in vivo nitrosatioi. to secondary
   and tertiary amines ingested from air, soil,
   water, and food, but the greatest potential for
   the formation of N-nitroso compounds and exposure
   to them appears to be in food.  In vivo formation
   of nitrosamines could be the largest contribution
   to body burden for the general population.

   A.  Sludge

       1.  Frequency of Detection

           Detected in 8 out of 16 and                Brewer et al.,
           quantified in 6 out of 16 municipal        1980 (p. 37)
           sludge samples from a single POTW.

           DMN was not identified in municipal        Naylor and
           sludge samples from 13 sites in            Loehr, 1982
           a 1980 study.  No occurrence of nitroso    (pp. 18 to 21)
           compounds was mentioned.

           DMN not found in sludges from 50 POTUs     U.S. EPA, 1982

       2.  Concentration

           0.272 Ug/g mean (WW) in 6 out of           Brewer et al.,
           16 municipal sludge samples                1980 (p. 37)

   B.  Soil - Unpolluted

       1.  Frequency of Detection

           No DMN found in 18 crop soil samples       West and Day,
           from seven states (D.L. = 0.2 ng/g)        1979 (p. 1078)

       2.  Concentration

           Data not immediately available.
                                 4-1

-------
C.  Hater - Unpolluted

    1.  Frequency of Detection

        No DMN found in Patapsco River
        downstream from contamination site

        No DMN found in six river and pond
        samples from five states (1977)

    2.  Concentration

        a.  Freshwater

            Data not immediately available.

        b.  Seawater

            Data not immediately available.

        c.  Drinking Hater

            No DMN found in Baltimore drinking
            water in 1975

            No DMN found in drinking water
            samples from Boston and Waltham,
            MA; New Orleans, Metairie and
            Mererro, LA, in 1975 at levels
            down to 10 Ug/L.

D.  Air

    1.  Frequency of Detection

        No DMN found in air samples from
        Waltham, MA; Philadelphia,  PA; and
        Wilmington, DE, at the part-pet—
        trillion level (1975) - 14  samples.

    2.  Concentration

        DMN levels in downtown Baltimore
        averaged 100 ng/m3 in November-
        December, 1975.

        DMN levels in Baltimore in  August,
        1975,  averaged 670 ng/m-*; range:
        ND to  2,908.66 ng/m3

        DMN levels in Belle, WV, in August
        1975,  averaged 59 ng/m3; range:
        trace  to ISA.5 ng/m3
Fine et  al.,
1977 (p.  582)

West and  Day,
1979 (p.  1077)
Fine et al.,
1977 (p. 582)

Fine et al.,
1975 (p. 406)
Fine et al.,
1976 (p. 1328)
Fine et al.,
1976 (p. 582)
Fine et al.,
1976 (p. 1328)
Fine et al.,
1976 (p. 1328)
                              4-2

-------
E.  Pood
    1.  Total Average Intake

        "The principal sources of preformed
        nitrosamines appear Co be diet and
        tobacco smoke."

        On the basis of one experiment, an
        average human dose rate for a 70 kg
        adult is 0.06 Ug/kg body weight
        representing an average exposure
        equivalent to approximately 2.2 ng/g
        of total nitrosamines assuming 2 kg/
-------
II. HUMAN EFFECTS

    A.  Ingestion

        1.  Carcinogenic!ty

            a.  Qualitative Assessment

                Epidemiclogical studies have failed    U.S. EPA, 1980
                to establish a direct relationship     (pp- C-43 to
                between exposure to N-nitroso          C-46)
                compounds and the development of
                human cancer.  However, the demon-
                strated ability of N-nitroso
                compounds to produce cancer in a
                wide range of experimental animals,
                combined with the capacity of
                human liver tissue to metabolize
                N-nitroso compounds to aikylating
                and mutagenic forms, strongly suggest
                that these compounds may be human
                carcinogens.  Guidelines for human
                exposure to N-nitrosamines are based
                on the assumption that these com-
                pounds are human carcinogens.

            b.  Potency

                Cancer potency of                      U.S. EPA, 1980
                25.88 (mg/kg/day)'1 has been           (C-6A)
                estimated for DMN.  The value is
                based on lifetime exposure of
                rats to N-nitrosamine compounds.

            c.  Effects

                Data not immediately available.

        2.  Chronic Toxicity

            a.  ADI

                Not applicable for this assessment.

            b.  Effects

                One man exposed to DMN contained in    U.S. EPA, 1980
                an industrial solvent exhibited        (p.  C-20)
                signs of liver damage.  Two of three
                men exposed to DMN while employed in
                an industrial research laboratory for
                10 months showed signs of liver
                injury.  The exact route of exposure
                of above individuals is not reported.
                                  4-4

-------
         3.  Absorption Factor

             Data not immediately available.

         4.  Existing Regulations

             An interim target risk level  of  10"^
             (a probability of one additional case
             of cancer for every 1,000,000 people
             exposed) for DMN has been set for
             drinking water:  0.0014 Ug/L

     B.  Inhalation

         1.  Carcinogenicity

             See Section 4, 4-4.

         2.  Chronic Tozicity

             See Section 4, 4-4.

         3.  Absorption Factor

             Data not immediately available.

III. PLANT EFFECTS

     A.  Phytotozicity

         Data not immediately available.

     B.  Uptake

         "Nitrosamines adsorbed by plants  disappear
         rapidly."

         No detectable radioactivity in stems,
         leaves and beans  from soybean plants
          frown in soil containing 0.1  Mg/g of
          4C DMN
 U.S.  EPA,  1980
 (C-48)
West and Day,
1979 (p. 1080)

West and Day,
1979 (p. 1080)
                                  4-5

-------
        Uptake cf DMN by spinach and lettuce:
Dean-Raymond
and Alexander,
1976 (p. 395)
Plant
Lettuce
Lettuce
Spinach
Lettuce

14C-DMN Length of
Growth Supplied Exposure
Medium (ugCi) (days)
Sand 0.057
0.57
Soil 0.57
Water 0.057
0.57
Sand 0.57

2
2
2
2
2
4
9
15
DMN Z DMN
Taken Up* Taken Up
(ug/g DW) By Plant*
1.38
14.38
106.0
0.54
5.60
7.04
1.40
0.07
3.20
3.25
5.06
0.38
0.27
1.56
0.21
0.02
        *Each figure represents the average of four replicates.
IV. DOMESTIC ANIMAL AND WILDLIFE EFFECTS

    A.  Toxicity

        See Table 4-1.

        Toxic levels of DMN in pigs and poultry
        are at least ten times greater than in
        cows, sheep, and mink.

        Cows fed a diet amended with pure DMN
        in a concentration of 50 ppm for 480
        days showed occlusion of some hepatic
        veins and neoformation of others.

    B.  Uptake

        "DMN accumulated (in cows) when the dosage
        in diet exceeded 0.1 mg/kg body weight."

 V. AQUATIC LIFE EFFECTS

    A.  Toxicity

        1.  Freshwater

            a.  Acute

                Available data is limited to acute
                values for Daphnia magna and blue
Koppang, 1974
(p. 526)
Koppang, 1974
(p. 524-525)
Koppang, 1974
(p. 523)
U.S. EPA, 1980
(p. B-l)
                                  4-6

-------
                 gill exposed to N-nitrosodiphenyla-
                 raine.  These values indicate that
                 toxicity due to N-nitroso compounds
                 may be as low as 5,850 Ug/L.

             b.  Chronic

                 Data not immediately available.

         2.  Saltwater

             a.  Acute

                 Acute 96 hour LC5Q for N-nitroso-      U.S. EPA, 1980
                 diphenylamine to the mummichog is      (p. B-l)
                 3,300,000 pg/L.

             b.  Chronic

                 Data not immediately available.

     B.  Uptake

         Bioconcentration factor for N-nitrosodi-       U.S. EPA, 1980
         phenylamine by blue gill was 217.  The         (p. B-l)
         half-life of the compound was estimated
         to be less than one day.

 VI. SOIL BIOTA EFFECTS

     Bacteria are not capable of activating N-          NAS, 1978
     nitroso compounds without supplementation          (p. 454)
     with animal-derived enzymes.

VII. PHYSICOCHEMICAL DATA FOR ESTIMATING PATE AND TRANSPORT

     DMN appears when soils and waters are amended      Mills and
     with nitrate or nitrite and either dimethyl-       Alexander,
     mine (DMNA) or trimethylamine.  Nitrosation        1976
     is enhanced by acidic conditions.                  (p. 437, 440)

     Recovery efficiencies for DMN are substan-         West and Day,
     tially lower than for the higher molecular         1979 (p. 1077)
     weight nitrosamines.

     Nitrosamines undergo rapid degradation and in      West and Day,
     lab soil studies have a half-life of 2 to 3        1979 (p. 1080)
     weeks, primarily due to rapid volatilization.

     Molecular weight:  74.08                           Weast, 1980
     Boiling point:  154°C                              (p. C-107)
     Soluble in water, alcohol and ether
                                   4-7

-------
Organic carbon partition coefficient:  0.04 mL/g   Hr.ssett et al.,
                                                   198:

Persistence:
- Nitrosamines were most stable in lake            Tate and
  water—no degradation or loss for 3.5            Alexander,
  months.                                          1975
- Slow disappearance in soil after a lag           (pp. 328 and
  of several weeks.                                329)
- Loss more rapid in sewage, but half of
  the nitrosamines remained after two
  weeks.
- Experiments with sterilized sewage
  indicate that nonbiologic factors are
  largely or entirely responsible for
  nitrosamine disappearance.
- Approximately 50 percent of initial dose
  of 25 ppm DMN persisted in Williamson
  silt loam 50 days after initial
  application.
                             4-8

-------
TABLE 4-1.  TOXICITY OP DIHETHYLNITROSANINE TO DOMESTIC AMIHALS AND WILDLIFE
Chemical Form
Species (N)a Fed
Rat (10)
Guinea pig
Li card (10)
Cat (6)
Monkey (6)
Duck (6)
Rat (10)
Guinea pig (10)
Lizard (10)
Cat (6)
Monkey (6)
Duck (6)
Rat (10)
Guinea pig (10)
Lizard (10)
DMN
DMN
DMN
DMN
DHN
DMN
DMN
DMN
DMN
DMN
DMN
DMN
DMN
DMN
DMN
Feed
Concentration
(mg/g)
NRD
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
Water
Concentration
(ng/L)
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
NR
Daily Intake Duration
(mg/kg) of Study
SO
SO
SO
SO
SO
SO
S
s
s
s
s
s
1
1
1
single dose
single dose
single dose
•ingle dose
single dose
•ingle dose
11 days
11 days
11 days
11 days
11 days
11 days
30 days
30 days
30 days
Effects
Liver damage, impaired
breathing, hemorrhage,
death at 9 days
Liver damage, impaired
breathing, hemorrhage,
death at S days
No apparent effect
Acute liver damage
Liver damage
No apparent effect
Liver damage
30Z mortality
Liver damage
40Z mortality
No apparent effect
Severe liver damage
66Z mortality
Severe liver damage
SOZ mortality
No apparent effect
Low weight gain
Low weight gain
Low weight gain
Reference -.
Haduagwu and
Bassir, 1980
(p. 213-14)















-------
                                                               TABLE 4-1.   (continued)
Chemical Form
Species (N)a Fed
Cat (10)
Honkey (10)
Duck (10)
Chinese hamster
(106)
Chinese hamster
Chinese hamster
** Hink
M
O
Bat
Bat (23)
Bat (12)
Cattle (23)
Cattle (23)
DNN
DHN
DNN
DMM
DNN
DNN
DNN
DNN
DNN
DNN
DNN
DNN
Peed
Concentration
(mg/g)
NR
NB
NB
NB
NB
NB
NB
NB
NB
NB
NR
NB
Uater
Concentration
(rag/L)
NR
NB
NB
NB
NB
NB
NR
NR
NR
NR
NR
NB
Daily Intake Duration
(mg/kg) of Study Effects
1 30 days Weight loss
SOX mortality
1 30 days No apparent effect
1 30 days No apparent effect
O.S1 6-20 months 6SZ reduction in survival
time
0.2S 6-20 months 64Z reduction in survival
time
0.13 6-20 months S7X reduction in survival
time. Liver tumor incidence
was 80-100Z at low and high
dosages
O.OSO twice per week Halignant tumors
40 twice per week LDjg
1.2 twice per week 65Z tumor incidence
6.0 twice per week 83Z tumor incidence
<0.1 7-70 weeks No clinical toxic effect
even if total intake
>40-58 mg/kg
>0.2 7-70 weeks Total intake of 12-26 mg/kg
caused serious disease and
death
References



Reznik et
1976 (p.





al..
412)


HAS, 197B
(p. 458)
U.S. EPA,
(p. C-21)
U.S. EPA,
(p. C-34)
U.S. EPA,
(p. C-34)
Koppang,
(p. 526)

1980
1980
1980
1974

• N = Number of experimental animals when reported.
b NH = Not reported.

-------
                                SECTION 5

                                REFERENCES
Abramowitz,  M.,  and  I.  A.  Stegun.    1972.    Handbook  of Mathematical
     Functions.  Dover Publications, New York, NY.

Bert rand,  J.  E.,  H.  C.  Lutrick,  G.  T.  Edds,  and R.  L.  West.   1981.
     Metal Residues  in Tissues, Animal  Performance and  Carcass Quality
     with Beef Steers  Grazing  Pensacola  Bahiagrass Pastures Treated with
     Liquid Digested Sludge.  J. Ani. Sci.  53:1.

Boswell,  P.  C.    '975.   Municipal  Sewage Sludge and  Selected Element
     Applications  to  Soil:   Effect  on Soil  and  Pescue.    J.  Environ.
     Qual.  4(2):267-273.

Brewer,  W.,  W.  S. Draper,  and S.  S. Wey.    1980.    The Detection  of
     Diraethylnitrosamine  and  Diethylnitrosamine   in   Municipal  Sewage
     Sludge  Applied  to  Agricultural Soils.   Env.  Pollut.  (Series  B).
     1:37-43.

Camp Dresser  and McKee,  Inc.   1984.   Development of  Methodologies  for
     Evaluating  Permissible  Contaminant Levels  in Municipal  Wastewater
     Sludges.  Draft.   Office of  Water  Regulations and  Standards,  U.S.
     Environmental  Protection Agency,-Washington, D.C.

Chaney,  R.  L.,  and  C.  A.   Lloyd.    1979.    Adherence of  Spray-Applied
     Liquid Digested  Sewage  Sludge  to  Tall Pescue.   J. Environ.  Qual.
     8(3):407-411.

Dean-Raymond, D., and M. Alexander.   1976.  Plant  Uptake  and  Leaching of
     Dimethylnitrosamine.  Nature.   262:394-396.

Donigian, A.  S.  1985.   Personal  Communication.  Anderson-Nichols  &  Co.,
     Inc., Palo Alto,  CA.  May.

Freeze, R. A., and J.  A.  Cherry.    1979.   Groundwater.   Prentice-Hall,
     Inc., Englewood  Cliffs,  NJ.

Fiddler,  W.,  J.  Feinberg,  J. W.  Pensabene  et  al.    1975.    Dimethyl-
     Nitrosamine  in Souse and  Similar Jellied Cured-Meat Products.   Fd.
     Cosmet.  Toxiol.   13:653-654.

Fine,  E.,  D.  P.  Rounbehler,  F.   Huffman  et  al.    1975.   Analysis  of
     Volatile N-nitroso  Compounds  in Drinking  Water  at  the  Part  Per
     Trillion Level.   Bull Env. Contam.  Tox.  14(4):404-408.

Fine, E., D.  P. Rounbehler, N. M. Belcher, and S.  S. Epstein.   1976.   N-
     nitroso  Compounds:   Detection  in  Ambient Air.   Science  192:1328-
     1330.
                                   5-1

-------
 Fine,  E.,  D. P. Rounbehler,  A. Rounbehler et  aL.   1977.   Determination
     of  Dimethylnitrosamine  in Air, Water,  and Soil  by Thermal  Energy
     Analysis:   Measurements  in Baltimore,  Maryland.    Env. Sci.  Tech.
     ll(6):581-584.

 Gelhar,  L.  W.,  and  G.   J.  Axness.    1981.    Stochastic  Analysis  of
     Macrodispersion  in 3-Dimensionally Heterogeneous  Aquifers.   Report
     No.  H-8.   Hydrologic  Research  Program,   New Mexico  Institute  of
     Mining  and Technology, Soccorro, NM.

 Gerritse,  R. G.,  R. Vriesema,  J.  W. Dalenberg  and  H.  P. DeRoos.   1982.
     Effect  of Sewage  Sludge  on  Trace Element  Mobility in  Soils.    J.
     Environ. Qual. 2:359-363.

 Griffin,  R.  A.   1984.    Personal  Communication  to U.S.   Environmental
     Protection  Agency,   ECAO  -   Cincinnati,  OH.     Illinois  State
     Geological Survey.

 Hassett, J.  J., W.  L.  Banwart,  and R.  A. Griffin.  1983.  Correlation  of
     Compound  Properties  with  Sorption  Characteristics   of   Non-polar
     Compounds  by  Soils  and  Sediments:    Concepts  and   Limitations.
     Chapter  IS.   In:   The Environment  and  Soil Waste Characterization,
     Treatment, and Disposal.   C.  W. Francis and  I.  Auerbach, eds.  Ann
     Arbor  Science  Pub.   Proc. 4th Oak Ridge  National  Laboratory Life
     Science Symposium.  October 4.  Gatlinburg, TN.

 Havery,  D.,  D. A.  Kline,  E.  M. Miletta et  al.   1976.  Survey of Food
     Products  for  Volatile  N-nitrosamines.    Journal   of   the  A.O.A.C.
     59(3):540-546.

 Koppang, N.   1974.   Toxic Effects  of  Dimethylnitrosamine   in Cows.    J.
     Nat. Cancer Inst.  52(2):523-528.

 Maduagwu,  E.,  and  0.  Bassir.    1980.   A Comparative  Assessment  of Toxic
     Effects  of  Dimethylnitrosamine in  Six  Different  Species.    Tox.
     Appl. Pharm.   53:211-219.

 Mills,    A.  L.,   and  M.   Alexander.      1976.     Factors   Affecting
     Dimethylnitrosamine Formation  in  Samples   of  Soil  and Water.   J.
     Environ. Qual.  5(4):437-440.

 National  Academy   of  Sciences.    1978.   Nitrates:    An  Environmental
     Assessment.      National    Research  Council   Panel   on   Nitrates.
     Washington,  D.C.

 Naylor,  L.,  and  R. Loehr.    1982.    Priority  Pollutants   in  Municipal
     Sewage Sludge.  BioCycle.   July/Aug, 18-22.

 Pennington, J.A.T.  1983.   Revision of  the  Total Diet  Study  Food  Lists
     and Diets. J.  Am.  Diet.  Assoc.   82:166-173.

Pettyjohn,  W. A., D.  C.  Kent,  T. A. Prickett,  H.  E. LeGrand, and  F.  E.
     Witz.     1982.     Methods   for  the  Prediction  of  Leachate   Plume
     Migration and  Mixing.    U.S.  EPA  Municipal Environmental  Research
     Laboratory, Cincinnati, OH.

                                   5-2

-------
Reznik,  G., U.  Mohr,  and  N.  Knoch.   1976.   Carcinogenic  Effects  of
     Different    Nitroso-compouna*    in   Chinese    Hamsters.        I.
     Dimethylnitrosamine  and   '-Diethylnitrosamine.     Br.  J.  Cancer.
     33:411-418.

Ryan, J.  A., H.  R. Pahren,  and J. B.  Lucas.   1982.   Controlling Cadmium
     in  the Human Food Chain:   A Review  and Rationale  Based  on Health
     Effects.  Environ. Res.  28:251-302.

Sikora,  L.  J., W. D.  Burge,  and  J.  E. Jones.   1982.   Monitoring  of a
     Municipal Sludge Entrenchment Site.   J.  Environ. Qual.  2(2):   321-
     325.

Tate, R.,  and  M.  Alexander.  -1975.   Stability of Nitrosamines  in  Lake
     Water, Soil, and Sewage.  Mat. Cancer Inst.  54(2):327-330.

Thornton, I., and  P. Abrams.   1983.   Soil  Ingestion  - A Major Pathway  of
     Heavy Metals  into  Livestock Crazing Contaminated Land.  Sci.  Total
     Environ.  28:287-294.

U.S.  Department   of  Agriculture.     1975.     Composition   of  Foods.
     Agricultural Handbook No. 8.

U.S. Environmental  Protection Agency.   1977.   Environmental  Assessment
     of  Subsurface  Disposal  of Municipal  Wastewater  Sludge:    Interim
     Report.     EPA/530/SU-547.      Municipal  Environmental   Research
     Laboratory, Cincinnati, OH.

 U.S. Environmental  Protection  Agency.   1980.    Ambient  Water  Quality
     Criteria  for  Nitrosamines.    EPA 440/5-80-064.    U.S.  Environmental
     Protection Agency, Washington, D.C.

U.S.  Environmental  Protection  Agency.    1982.     Fate   of   Priority
     Pollutants  in  Publicly-Owned   Treatment   Works.     Final  Report.
     Vol. 1.     EPA   440/1-82-303.      Effluent  Guidelines   Division,
     Washington, D.C.  September.

U.S.  Environmental  Protection  Agency.    1983a.   Assessment  of  Human
     Exposure  to  Arsenic:    Tacoma,   Washington.    Internal  Document.
     OHEA-E-075-U.    Office  of  Health  and  Environmental  Assessment,
     Washington, D.C.  July 19.

U.S.  Environmental  Protection  Agency.    1983b.   Rapid  Assessment  of
     Potential    Groundwater   Contamination   Under    Emergency   Response
     Conditions.  EPA 600/8-83-030.

U.S. Environmental Protection  Agency.   1984.   Air Quality  Criteria  for
     Lead.   External Review  Draft.    EPA  600/8-83-028B.   Environmental
     Criteria  and   Assessment   Office,  Research  Triangle  Park,   NC.
     September.

Weast,   R.    1980.   Handbook  of  Chemistry  and Physics.    CRC  Press,
     Cleveland, OH.
                                   5-3

-------
West, S., and E.  Day.   1979.  Determination of  Volatile  Nitrosamines in
     Crops and Soils Treated with Dinitroaniline Herbicides.   J. Agric.
     Food Chera.   27(5):1075-1080.
                                   5-4

-------
                                 APPENDIX

      PRELIMINARY HAZARD INDEX CALCULATIONS PO* DIMETHYL MITROSAMINE
                        IN MUNICIPAL SEWAGE SLUDGti
  I. LANDSPREADING AND DISTRIBUTION-AND-MARKETING

     A.  Effect on Soil Concentration of Dimethyl Nitrosamine

         1.  Index of Soil Concentration (Index 1)

             a.  Formula

                       (SC x AR) + (BS x MS)
                 C5S "        AR + MS

                 CSr = CSg  [1 +  0.5 + ... +

                 where:

                      CSg = Soil  concentration  of   pollutant   after  a
                            single   year's   application    of    sludge
                            (Ug/g DU)
                      CSr = Soil  concentration of  pollutant  after  the
                            yearly   application   of   sludge  has   been
                            repeated for n + 1 years (ug/g DW)
                      SC  = Sludge concentration of pollutant (ug/g DW)
                      AR  = Sludge application rate (rat/ha)
                      MS  = 2000  mt  ha/DW =  assumed  mass  of  soil  in
                            upper 15 cm
                      BS  = Background  concentration   of   pollutant   in
                            soil (Ug/g DW)
                      t|  = Soil half-life of pollutant (years)
                      n   =99 years

             b.  Sample calculation

                 CSS is calculated for AR = 0, 5,  and  SO mt/ha only


         n nn*A „ /  nu - (2.55 Ug/g DW x  5 mt/ha) + (0 Ug/g DW x 2000 mt/ha)
         0.0064 ug/g DW -           (5  mt/ha DW +  2000 mt/ha DW)	

                 CSr is calculated for AR = 5 mt/ha applied for  100 years


0.0064 ug/g DW = 0.0064 ug/g DW [1 + 0.5(1/0>14) + 0.5(2/0<14> + ...  +
                                Qt5(99/0.14)]
                                   A-l

-------
B.  Effect on Soil Biota and Predators of Soil Biota

    1.  Index of Soil Biota Toxicity (Index 2)

        a.  Formula

                      II
            Index 2 = —


            where:

                 1}  = Index 1 = Concentration of pollutant in
                       sludge-amended soil (ug/g DW)
                 TB  = Soil  concentration   toxic  to   soil   biota
                       (Ug/g DU)

        b.  Sample calculation -  Values  were not calculated  due to
            lack of data.

    2.  Index of Soil Biota Predator Toxicity (index 3)

        a.  Formula

            in,., 3 = i^JE


            where:

                 1}  = Index 1  = Concentration of pollutant in
                       sludge-amended soil  (ug/g DU)
                 UB  = Uptake  factor of  pollutant  in  soil   biota
                       (pg/g tissue DW [yg/g soil DW]"1)
                 TR  = Feed concentration  toxic  to  predator  (pg/g
                       DW)

        b.  Sample calculation -  Values  were  not calculated  due  to
            lack of data.

C.  Effect on Plants and Plant  Tissue Concentration

    1.  Index of Phytotoxic Soil  Concentration (Index 4)

        a.  Formula


            Index 4 = —
            where:
                 1}   = Index  1  = Concentration of  pollutant  in
                       sludge-amended  soil  (pg/g DW)
                 TP   = Soil concentration toxic  to plants  (pg/g  DW)
                             A-2

-------
        b.  Sample calculation  -  Values were not  calculated due to
            lack of data.

    2.  Index of Plant Concentration Caused by Uptake (Index 5)

        a.  Formula

            Index 5 = Ij x UP

            where:

               1} = Index 1 = Concentration of pollutant in
                   sludge - amended soil (ug/g DW)
               UP = Uptake factor of pollutant in piant tissue
                     (llg/g tissue DW [llg/g soil  DW]'1)

        b.  Sample Calculation  -  Values were not  calculated  due to
            lack of data.

    3.  Index  of  Plant  Concentration  Permitted  by  Phytotoxicity
        (Index 6)

        a.  Formula

            Index 6 = PP

            where:

                 PP  = Maximum  plant  tissue  concentration  associ-
                       ated with phytotoxicity (Ug/g DW)

        b.  Sample calculation  -  Values were not  calculated  due to
            lack of data.

D.  Effect on Herbivorous Animals

    1.  Index of  Animal Toxicity  Resulting  from  Plant  Consumption
        (Index 7)

        a.  Formula

            index 7 = ^

            where!

                 15  = Index  5   =  Concentration  of  pollutant  in
                       plant grown in  sludge-amended soil (pg/g DW)
                 TA  = Feed  concentration   toxic    to   herbivorous
                       animal (ug/g DW)

        b.  Sample calculation -  Values  were not calculated due  to
            lack of data.
                             A-3

-------
    2.  Index  of  Animal  Toxicity Resulting  from Sludge  Ingestion
        (Index 8)

        a*  Formula

            If AR - 0; Index 8=0


            If AR * 0; Index 8 -  SC x GS
                                   TA
            where :
                 AR  = Sludge application rate (mt DW/ha)
                 SC  = Sludge concentration of pollutant (ug/g OW)
                 GS  = Fraction of animal diet assumed to be soil
                 TA  = Feed  concentration   toxic  to   herbivorous
                       animal 
-------
2.  Index  of  Human  Cancer Risk  Resulting from  Consumption c«T
    Animal  Products  Derived  from  Animals  Feeding  on  Pit-its
    (Index 10)

    a.  Formula

                    (15  x UA x DA) -i- DI
        Index 10 =

        where:

             Is  = Index  5  =  Concentration  of  pollutant  in
                   plant grown in sludge-amended soil (ug/g  W)
             UA  - Uptake  factor  of pollutant  in  animal tis'sue
                   (yg/g  tissue DW  [ug/g feed  DW]'1)
             DA  = Daily   human   dietary  intake   of  affected
                   animal  tissue  (g/day DW) (milk products and
                   meat, poultry, eggs,  fish)
             DI  = Average daily human dietary intake of
                   pollutant (pg/day)
             RSI = Cancer risk-specific  intake (ug/day)

    b.  Sample   calculation   (toddler)   -   Values  were   not
        calculated due  to lack of data.

3.  Index  of  Human  Cancer Risk  Resulting from  Consumption of
    Animal  Products Derived from Animals  Ingesting Soil (Index
    11)

    a.  Formula
                 n                 (BS x GS x  UA x DA)  + DI
        If  AR =  0;  Index 11 = 	jjgj	'	

               ,  „   T J    „      (SC x CS x  UA x DA)  + DI
        If  AR #  0;  Index 11 =

        where:

             AR  =  Sludge application  rate (mt DW/ha)
             BS  =  Background   concentration   of  pollutant  in
                    soil (yg/g  DW)
             SC  =  Sludge concentration  of pollutant  (ug/g  DW)
             GS  =  Fraction of animal  diet assumed to be  soil
             UA  =  Uptake  factor  of pollutant  in animal tissue
                    (Ug/g tissue DW [ug/g feed  DW]"1)
             DA  -  Daily   human  dietary  intake   of  affected
                    animal  tissue  (g/day DW)  (milk products and
                    meat only)
             DI  =  Average  daily human dietary  intake of
                    pollutant  (ug/day)
             RSI »  Cancer  risk-specific  intake  (lag/day)

    b.  Sample   calculation   (toddler)   -   Values   were   not
        calculated  due  to  lack of data.
                           A-5

-------
4.  Index  of  Human Cancer  Risk Resulting  from Soil  Ingestion
    (Index
    a •  Formula

                   (Ii x DS) *  DI
        Index 12 . _i_ -


        where :

             II  = Index 1  = Concentration   of   pollutant   in
                   sludge-amended soil (ug/g DW)
             OS  = Assumed  amount of soil in human diet (g/day)
             DI  = Average  daily human dietary intake of
                   pollutant (ug/day)
             RSI = Cancer risk-specific intake dig/day )

    b.  Sample calculation  (toddler)


        ,,n _ (0.0064 ue/g  DW x 5 g/day) * 0.67 ug/day
                       0.0027 yg/day


5.  Index of Aggregate Human Cancer Risk (Index 13)

    a.  Formula
        Index 13 = I9 * I10 * In  *  J12  -  <>-

        where :

             Ig  = Index   9 =  Index  of  cancer  risk  resulting
                   from plant  consumption (unitless)
             IIQ = Index  10 =  Index  of  cancer  risk  resulting
                   from consumption  of  animal products  derived
                   from animals  feeding on  plants  (unitless)
             111 = Index 11 =  Index  of  cancer  risk  resulting
                   from consumption  of  animal products  derived
                   from animals  ingesting soil (unitless)
             Il2 = Index 12 =  Index  of  cancer  risk  resulting
                   from soil  ingestion (unitless)
             DI  = Average  daily   human  dietary   intake   of
                   pollutant  (ug/day)
             RSI = Cancer  risk-specific  intake (ug/day)

    b.  Sample    calculation   (toddler)  -   Values   were   not
        calculated due to  lack of data.
                          A-6

-------
II .  LANDPILLING

     A.  Procedure

         Using Equation  1,  several  values  of  C/C0  for  the  unsaturated
         zone are  calculated  corresponding to  increasing values  of  t
         until equilibrium  is reached.   Assuming a  5-year pulse  input
         from the landfill, Equation  3  is employed to estimate  the  con-
         centration  vs. time data at the  water  table.   The concentration
         vs.  time curve is then transformed into a square pulse  having a
         constant concentration  equal  to  the  peak  concentration,   Cu,
         from the unsaturated  zone,  and  a  duration,  t0, chosen so  that
         the   total  areas  under  the  curve  and  the pulse are equal,  as
         illustrated in Equation  3.   This  square  pulse  is then used  as
         the   input  to  the  linkage  assessment,  Equation 2, which esti-
         mates initial  dilution in the  aquifer  to give  the initial  con-
         centration, C0, for the saturated  zone assessment.   (Conditions
         for   B,  minimum thickness  of  unsaturated  zone,  have  been  set
         such that dilution is actually negligible.)  The saturated  zone
         assessment  procedure is nearly identical to that  for the  unsat-
         urated  zone except for the definition of certain parameters  and
         choice  of  parameter  values.    The  maximum concentration at  the
         well, Cmax,  is used  to  calculate the  index   values  given  in
         Equations 4 and 5.

     B.  Equation 1: Transport Assessment
      C(y.t) =i [exp(Ai)  erfc(A2) + exp^) erfc(B2)]
       Co

          Requires evaluations  of  four  dimensionless  input  values  and
          subsequent  evaluation  of . the  result.    Exp(A^)  denotes  the
          exponential    of  AI,   e  *,   where   erfc(A2)   denotes   the
          complimentary error function  of  A2.   Erfc(A2) produces  values
          between 0.0 and  2.0  (Abramowitz  and Stegun,  1972).

          where:
               Al  „ *_  [V* -  (V*2 + 4D* x u
               Al   20*

                    y - t (V*2 * 4D* x u*)*
                          (AD* x

               Bl  - X — [V* + (V*2 + 4D* x
                1
B2
                    2D*

                    y + t (V*2 * 4D* x
                          (4D* x t)i
          and  where  for  the  unsaturated zone:

               C0  =  SC x CF  =  Initial  leachate concentration  (ug/L)


                                  A-7

-------
           SC = Sludge  concentre-ion of pollutant (mg/kg DU)
           CF = 250  kg  sludge  .-olids/m3 leachate =
                PS x 1Q3
                1 -  PS

           PS = Percent  solids  (by weight)  of  landfilled  sludge  =
                20Z
            t - Time (years)
           X  = h =  Depth  to groundwater (m)
           D* = o x  V*  (m2/year)
            a - Dispersivity coefficient  (m)

           y* = —2__ (m/yea?;'>
                6 x  R
            Q = Leachate generation  rate  (m/year)
            6 = Volumetric water content  (unitless)

            R = 1 +  _dŁY. x Kd  =  Retardation factor (unitless)
                     0
        pdry = Dry  bulk density (g/mL)
           Kd = foc  x Koc (mL/g)
         foc = Fraction of organic  carbon  (unitless)
         Koc = Organic carbon partition  coefficient (mL/g)

           U* = 2SLJLJI (years)-l
            U = Degradation rate (day"1)

     and where for  the saturated zone:

           C0  = Initial  concentration  of  pollutant   in aquifer  as
                determined by Equation 2  (ug/L)
            t  = Time (years)
            X = AŁ = Distance  from well  to  landfill (m)
           D*  = a x  V*  (m2/year)
           a = Dispersivity coefficient  (m)

           V*  =  K * * (m/year)
               0 x R
           K  = Hydraulic conductivity of the  aquifer  (m/day)
           i  = Average hydraulic gradient  between landfill  and well
                (unitless)
           0 = Aquifer porosity (unitless)

           R =  i +  fdrjr x  Kd  = Retardation factor = 1  (unitless)
                     0
               since Kd =  foc x Koc  and foc is  assumed to  be zero
               for  the  saturated zone.

C.  Equation 2.  Linkage Assessment
                          Q x W	
          C0 = Cu x
                    365 [(K x i) * 0] x B
                              A-8

-------
     where:

          C0 = Initial  concentration  of pollutant  in  the saturated
               zone as determined by Equation 1 (ug/L)
          Cu = Maximum  pulse  concentration  from  the  unsaturated
               zone (pg/L)
           Q = Leachate generation rate (m/year)
           H = Width of landfill (m)
           K = Hydraulic conductivity of the aquifer (m/day)
           i = Average hydraulic gradient  between  landfill  and well
               (unitless)
           0 = Aquifer porosity (unitless)
           B = Thickness of saturated zone (m) where:

               B ^  K  xVxW36V -  and B ^ 2

D.  Equation 3.  Pulse Assessment


          C(X'° = P(Xit)  for  0  <  t < t0
             co
             *   = P(X,C) -  P(x,t  - t0)  for t > t0
             co
     where :
          C0 (for  unsaturated  zone) = LT  = Landfill leaching  time
          (years)

          to (for  saturated zone)  =  Pulse duration at  the water
          table (x = h) as determined by the following equation:

               t0 = [  Q/*  C dt] *  Cu
                   C(Y t )
          P(X»t) = — *Ł* — as determined  by  Equation  1
                     uo
B.   Equation 4.  Index of Groundwater Concentration   Resulting
     from LandfiLled Sludge  (Index  1)

     1.   Formula

          Index 1 = Cmax

          where:

               Cgigx = Maximum  concentration of pollutant  at  well =
                     maximum  of C(AH,t) calculated  in  Equation 1
                     (Mg/L)

     2.   Sample Calculation

          9.02xlO~4 Ug/L = 9.02xlO~4 ug/L
                             A-9

-------
     P.   Equation S.  Index of Human Cancer  Risk Resulting
          from Groundwater Contamination (Index

          1.   Formula

                          Ul x AC) + DI
               Ind«2=  -4s!	

               where:

                    II = Index  1  =  Index  of groundwater  concentration
                         resulting  from  landfilled sludge (ug/L)
                    AC = Average  human  consumption  of  drinking  water
                         (L/day)
                    DI = Average daily human  dietary  intake  of pollutant
                         (pg/day)
                   RSI = Cancer risk-specific intake (ug/day)

          2.    Sample Calculation

                       (9.02x10"*  ug/L x 2  L/dav) +2.0  Ue/day
                 741               0.0027 Ug/day
III. INCINERATION
     Based on the  recommendations of  the  experts  at the OWRS  meeetings
     (April-May,  1984), an assessment  of  this reuse/disposal option  is
     not being conducted at this time.  The U.S. EPA reserves  the  right
     to conduct  such an assessment for this option  in the future.
IV.  OCEAN DISPOSAL

         i
     Based  on the recommendations of  the  experts  at the OWRS meeetings
     (April-May,  1984),  an assessment  of  this  reuse/disposal option  is
     not  being conducted  at  this time.  The U.S. EPA reserves the right
     to conduct such an assessment for this option  in the  future.
                                 A-10

-------
TABLE A-l.  INPUT DATA VARYING IN LANDFILL ANALYSIS AND RESULT FOR EACH CONDITION
Condition of Analysis
Input Data
Sludge concentration of pollutant, SC (mg/g DW)
Unsaturated tone
Soil type and characteristics
Dry bulk density, Pdry (g/mL)
Volumetric water content, 8 (unitless)
Fraction of organic carbon, foc (unitless)
Site parameters
Leachate generation rate, Q (m/year)
Depth to grounduater, h (m)
•^ Dispersivity coefficient, a (m)
*"* Saturated eone
Soil type and characteristics
Aquifer porosity, t (unitless)
Hydraulic conductivity of the aquifer,
K (m/day)
Site parameters
Hydraulic gradient, i (unitless)
Distance from well to landfill, Afi (o)
Dispersivity coefficient, a (m)
1
IT)
2.55

1.53
0.195
0.005

0.8
5
0.5


0.44
0.86

0.001
100
10
2
I"!
2.55

1.53
0.195
0.005

0.8
5
0.5


0.44
0.86

0.001
100
10
3
IT]
2.55

1.925
0.133
0.0001

0.8
5
0.5


0.44
0.86

0.001
100
10
4
IT]
2.55

NAb
MA
NA

1.6
0
MA


0.44
0.86

0.001
JOO
10
5
IT]
2.55

1.53
0.195
0.005

0.8
5
0.5


0.389
4.04

0.001
100
10
6
IT]
2.55

1.53
0.195
0.005

0.8
5
0.5


0.44
0.86

0.02
SO
5
7
IK]
2.SS

NA
NA
NA

1.6
0
NA


0.389
4.04

0.02
50
S
8
N*


N
N
N

N
N
N


M
H

N
N
N

-------
                                                                  TABLE A-l.  (continued)
10
Condition of Analysis
Results
Unsaturated cone assessment (Equations 1 and 3)
Initial leachate concentration, C0 (pg/L)
Peak concentration, Cu (pg/L)
Pulse duration, to (years)
Linkage assessment (Equation 2)
Aquifer thickness, B (m)
Initial concentration in saturated cone, C0
(pg/L)
Saturated cone assessment (Equations 1 and 3)
Maximum well concentration, Ł„&* (MS/D
Index of groundwater concentration resulting
from landfilled sludge. Index 1 (pg/L)
(Equation 4)
Index of human cancer risk resulting
from groundwater contamination, Index 2
(unitless) (Equation 5)
1

638
8.29
5.00

126
8.29

9.02x10-*
9.02x10-4
741
2

638
8.29
S.OO

126
8.29

9.2x10-4
9.0x10*4
741
3

638
25.6
5.00

126
25.6

2.78x10-3
2.78x10-3
743
4

638
638
5.00

253
638

6.93x10-2
6.93x10-2
792
5

638
8.29
S.OO

23.8
8.29

4.79x10-3
4.79x10-3
744
6

638
8.29
5.00

6.32
8.29

3.61x10-2
3.61x10-2
767
7

638
638
5.00

2.38
6.38

14.8
14.8
11700
8

N
N
H

H
H

N
0
(Mil
     •N   -  Null  condition, where no landfill exists; no value  is used.
     bNA  =  Not applicable  for this condition.

-------